LOCUS       DAKTGS010000001        87110 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.001, whole genome shotgun sequence.
ACCESSION   DAKTGS010000001 DAKTGS010000000
VERSION     DAKTGS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 87110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.001"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            247..648
                     /locus_tag="O0286_000001"
     CDS             247..648
                     /locus_tag="O0286_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0168406.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(768..1331)
                     /locus_tag="O0286_000002"
     CDS             complement(768..1331)
                     /locus_tag="O0286_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0168407.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1328..2281)
                     /locus_tag="O0286_000003"
     CDS             complement(1328..2281)
                     /locus_tag="O0286_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCY0168408.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2391..3572
                     /locus_tag="O0286_000004"
     CDS             2391..3572
                     /locus_tag="O0286_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0168409.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3860..6277
                     /locus_tag="O0286_000005"
     CDS             3860..6277
                     /locus_tag="O0286_000005"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCY0168410.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6299..6610
                     /locus_tag="O0286_000006"
     CDS             6299..6610
                     /locus_tag="O0286_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0168411.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            6936..13496
                     /gene="sasC"
                     /locus_tag="O0286_000007"
     CDS             6936..13496
                     /gene="sasC"
                     /locus_tag="O0286_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCY0168412.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(13613..14881)
                     /locus_tag="O0286_000008"
     CDS             complement(13613..14881)
                     /locus_tag="O0286_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0168413.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            15288..16949
                     /locus_tag="O0286_000009"
     CDS             15288..16949
                     /locus_tag="O0286_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0168414.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            16946..17641
                     /locus_tag="O0286_000010"
     CDS             16946..17641
                     /locus_tag="O0286_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0168415.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            17658..18080
                     /locus_tag="O0286_000011"
     CDS             17658..18080
                     /locus_tag="O0286_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0168416.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            18830..20239
                     /gene="sapep"
                     /locus_tag="O0286_000012"
     CDS             18830..20239
                     /gene="sapep"
                     /locus_tag="O0286_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCY0168417.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            20243..21091
                     /gene="dat"
                     /locus_tag="O0286_000013"
     CDS             20243..21091
                     /gene="dat"
                     /locus_tag="O0286_000013"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCY0168418.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            21641..22432
                     /locus_tag="O0286_000014"
     CDS             21641..22432
                     /locus_tag="O0286_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCY0168419.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            22447..23091
                     /gene="trmB"
                     /locus_tag="O0286_000015"
     CDS             22447..23091
                     /gene="trmB"
                     /locus_tag="O0286_000015"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCY0168420.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            23561..24403
                     /locus_tag="O0286_000016"
     CDS             23561..24403
                     /locus_tag="O0286_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0168421.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(24528..24839)
                     /locus_tag="O0286_000017"
     CDS             complement(24528..24839)
                     /locus_tag="O0286_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0168422.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            24926..26002
                     /locus_tag="O0286_000018"
     CDS             24926..26002
                     /locus_tag="O0286_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0168423.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            26067..26378
                     /locus_tag="O0286_000019"
     CDS             26067..26378
                     /locus_tag="O0286_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0168424.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            26478..27335
                     /locus_tag="O0286_000020"
     CDS             26478..27335
                     /locus_tag="O0286_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCY0168425.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            27364..27960
                     /locus_tag="O0286_000021"
     CDS             27364..27960
                     /locus_tag="O0286_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCY0168426.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            27981..31805
                     /locus_tag="O0286_000022"
     CDS             27981..31805
                     /locus_tag="O0286_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0168427.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            31829..33142
                     /locus_tag="O0286_000023"
     CDS             31829..33142
                     /locus_tag="O0286_000023"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCY0168428.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            33216..33707
                     /locus_tag="O0286_000024"
     CDS             33216..33707
                     /locus_tag="O0286_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCY0168429.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            33781..35139
                     /locus_tag="O0286_000025"
     CDS             33781..35139
                     /locus_tag="O0286_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168430.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTF
                     ND"
     gene            35274..35378
                     /locus_tag="O0286_000026"
     CDS             35274..35378
                     /locus_tag="O0286_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168431.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
     gene            35839..36930
                     /locus_tag="O0286_000027"
     CDS             35839..36930
                     /locus_tag="O0286_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCY0168432.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
     gene            37284..37397
                     /locus_tag="O0286_000028"
     CDS             37284..37397
                     /locus_tag="O0286_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168433.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            37470..38459
                     /gene="ccpA"
                     /locus_tag="O0286_000029"
     CDS             37470..38459
                     /gene="ccpA"
                     /locus_tag="O0286_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCY0168434.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(38557..39726)
                     /locus_tag="O0286_000030"
     CDS             complement(38557..39726)
                     /locus_tag="O0286_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCY0168435.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(39751..40383)
                     /locus_tag="O0286_000031"
     CDS             complement(39751..40383)
                     /locus_tag="O0286_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0168436.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            40552..42258
                     /gene="acsA"
                     /locus_tag="O0286_000032"
     CDS             40552..42258
                     /gene="acsA"
                     /locus_tag="O0286_000032"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCY0168437.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            42698..44365
                     /locus_tag="O0286_000033"
     CDS             42698..44365
                     /locus_tag="O0286_000033"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCY0168438.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            44661..47348
                     /gene="harA"
                     /locus_tag="O0286_000034"
     CDS             44661..47348
                     /gene="harA"
                     /locus_tag="O0286_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCY0168439.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            47479..47571
                     /locus_tag="O0286_000035"
     CDS             47479..47571
                     /locus_tag="O0286_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168440.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(47551..48456)
                     /locus_tag="O0286_000036"
     CDS             complement(47551..48456)
                     /locus_tag="O0286_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0168441.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            48838..50100
                     /locus_tag="O0286_000037"
     CDS             48838..50100
                     /locus_tag="O0286_000037"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCY0168442.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(50194..51468)
                     /locus_tag="O0286_000038"
     CDS             complement(50194..51468)
                     /locus_tag="O0286_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0168443.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            51640..52257
                     /locus_tag="O0286_000039"
     CDS             51640..52257
                     /locus_tag="O0286_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCY0168444.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            52385..53851
                     /locus_tag="O0286_000040"
     CDS             52385..53851
                     /locus_tag="O0286_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCY0168445.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            53958..55088
                     /locus_tag="O0286_000041"
     CDS             53958..55088
                     /locus_tag="O0286_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0168446.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(55049..55144)
                     /locus_tag="O0286_000042"
     CDS             complement(55049..55144)
                     /locus_tag="O0286_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168447.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(55238..56842)
                     /locus_tag="O0286_000043"
     CDS             complement(55238..56842)
                     /locus_tag="O0286_000043"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0168448.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(56829..57989)
                     /locus_tag="O0286_000044"
     CDS             complement(56829..57989)
                     /locus_tag="O0286_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCY0168449.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(58103..58549)
                     /locus_tag="O0286_000045"
     CDS             complement(58103..58549)
                     /locus_tag="O0286_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCY0168450.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            58629..59372
                     /locus_tag="O0286_000046"
     CDS             58629..59372
                     /locus_tag="O0286_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0168451.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(59588..60190)
                     /gene="rpsD"
                     /locus_tag="O0286_000047"
     CDS             complement(59588..60190)
                     /gene="rpsD"
                     /locus_tag="O0286_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCY0168452.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(60434..60898)
                     /locus_tag="O0286_000048"
     CDS             complement(60434..60898)
                     /locus_tag="O0286_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCY0168453.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            61035..62729
                     /gene="ezrA"
                     /locus_tag="O0286_000049"
     CDS             61035..62729
                     /gene="ezrA"
                     /locus_tag="O0286_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCY0168454.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(62803..62943)
                     /locus_tag="O0286_000050"
     CDS             complement(62803..62943)
                     /locus_tag="O0286_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168455.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            63092..64240
                     /locus_tag="O0286_000051"
     CDS             63092..64240
                     /locus_tag="O0286_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0168456.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            64240..65463
                     /gene="thiI"
                     /locus_tag="O0286_000052"
     CDS             64240..65463
                     /gene="thiI"
                     /locus_tag="O0286_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCY0168457.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            65507..66277
                     /locus_tag="O0286_000053"
     CDS             65507..66277
                     /locus_tag="O0286_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCY0168458.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            66375..66869
                     /gene="tpx"
                     /locus_tag="O0286_000054"
     CDS             66375..66869
                     /gene="tpx"
                     /locus_tag="O0286_000054"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCY0168459.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            66992..67939
                     /locus_tag="O0286_000055"
     CDS             66992..67939
                     /locus_tag="O0286_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0168460.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            68027..69229
                     /locus_tag="O0286_000056"
     CDS             68027..69229
                     /locus_tag="O0286_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCY0168461.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(69475..69975)
                     /locus_tag="O0286_000057"
     CDS             complement(69475..69975)
                     /locus_tag="O0286_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0168462.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            70116..71234
                     /gene="ald"
                     /locus_tag="O0286_000058"
     CDS             70116..71234
                     /gene="ald"
                     /locus_tag="O0286_000058"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0168463.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            71358..71456
                     /locus_tag="O0286_000059"
     CDS             71358..71456
                     /locus_tag="O0286_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168464.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(71550..72605)
                     /locus_tag="O0286_000060"
     CDS             complement(71550..72605)
                     /locus_tag="O0286_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0168465.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            72780..73469
                     /locus_tag="O0286_000061"
     CDS             72780..73469
                     /locus_tag="O0286_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0168466.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(73774..74187)
                     /locus_tag="O0286_000062"
     CDS             complement(73774..74187)
                     /locus_tag="O0286_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0168467.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            74392..75690
                     /locus_tag="O0286_000063"
     CDS             74392..75690
                     /locus_tag="O0286_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCY0168468.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            75951..76892
                     /gene="pde2"
                     /locus_tag="O0286_000064"
     CDS             75951..76892
                     /gene="pde2"
                     /locus_tag="O0286_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCY0168469.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            76913..80110
                     /locus_tag="O0286_000065"
     CDS             76913..80110
                     /locus_tag="O0286_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0168470.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
     gene            80560..81789
                     /locus_tag="O0286_000066"
     CDS             80560..81789
                     /locus_tag="O0286_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCY0168471.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            81984..82841
                     /locus_tag="O0286_000067"
     CDS             81984..82841
                     /locus_tag="O0286_000067"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCY0168472.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            82841..83785
                     /locus_tag="O0286_000068"
     CDS             82841..83785
                     /locus_tag="O0286_000068"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCY0168473.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            84054..85022
                     /gene="pfkA"
                     /locus_tag="O0286_000069"
     CDS             84054..85022
                     /gene="pfkA"
                     /locus_tag="O0286_000069"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCY0168474.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            85044..86801
                     /gene="pyk"
                     /locus_tag="O0286_000070"
     CDS             85044..86801
                     /gene="pyk"
                     /locus_tag="O0286_000070"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCY0168475.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT        31226 a        12521 c        16114 g        27249 t
ORIGIN      
        1 attaatatgt aatagagtga tttgttttat gtactattat cttatttcta aatattaact
       61 ctattgatta ttggttttta tacttattta attttattca actttgacaa ttgaatagaa
      121 agcaagttta tttacacttg tagttttatg cataagttag cacatacaag ttttgggatt
      181 gttgggatgt ttgttaatac ttgtatagta gctaaatatg tgattattaa ttgggagatg
      241 tttagcatga aaaaagtaaa taacgacact gtatttggaa ttttgcaatt agaaacactt
      301 ttgggtgaca ttaactcaat tttcagcgag attgaaagcg aatacaaaat gtctagagaa
      361 gaaattttaa ttttactaac tttatggcaa aaaggttcta tgacgcttaa agaaatggac
      421 agatttgttg aagttaaacc gtataagcgt acgagaacgt ataataattt agttgaatta
      481 gaatggattt acaaagagcg tcctgttgac gatgaaagaa cagttattat tcatttcaat
      541 gaaaagttac aacaagagaa agtagagttg ttgaatttca tcagtgatgc gattgcaagt
      601 agagcaacag caatgcaaaa tagtttaaac gcaattattg ctgtgtaagt ttaatagcat
      661 aaaaagaggt tttcattaag ttgaaaacct ctttttgttg ttggcattaa tttttcaaat
      721 gttgactact caatcctaaa ttataaatag tatagcgcag caaatgctta agaaattttt
      781 tctatggcac aaatgaatgg agcatgatta cgttggttta aaaattgata ttgcaaaact
      841 tgcgcatgct tttgatccaa agtactcaag taatcaagca atgcatgctt ctcaatttgt
      901 ccttcgctat gaccatgata tataacaagt acaataatac cttcaattga cattaatgat
      961 agcaatgaat taatagcttg gattgtcgtg tcaggctttg tcacgataga tttatcacct
     1021 ttaggcaaat aacctaggtt aaagatggct gcatcaatat gacctttatg tgcatcattt
     1081 atatgatgtt caatattttc atgtccatct tttattaaag aaacatgatt gaaatcctta
     1141 actttatcac gtgtattttc caaagctaaa tcttgaatgt cgaaaccata aacatgtcct
     1201 tctggtactt gttcggctaa aaataaagtg tcattgccgt taccgcaagt tgcgtctaca
     1261 acaatacttt ctggtgttat atgttgttta ataagtgttt ttgaaaaagg gagtatacgt
     1321 tctaatttca ttgcttcacc ttagacttgt aacgcaagcc ttgataagaa tttctacgtg
     1381 ctaattcagc atcgatgcca tttaatactt cccatttatt aacactccac attggaccta
     1441 ccatgatatc tattggacca tcaccggtaa ttcggtgaac gatcatttca gggggaatca
     1501 cttctaattg gtccacaact aggtttgtgt actcttcttg agtcataaaa gttaataaac
     1561 ctttatcgta ttgttttacc atcggtgtac cttttaacaa atgaagtaaa tgaattttaa
     1621 taccttgtac atccatttgt gcaacttctt tggcagtagc catcatcatg tcatagtctt
     1681 cgccaggtaa gccattaatg atgtgtgtac atacattgat attatgctta cgtaattttg
     1741 ccacaccatc ataataagtt ttcatatcat gggcacgatt gattaaatca gatgttgact
     1801 gatggattgt ttgtagtcct aattcaaccc ataagtatgt tcgttgattc aaatctgcta
     1861 aatattcgac aacatcgtct ggtagacagt caggacgcgt accaatagat aatcccacaa
     1921 cacccggttc tttaagtaca ggttcgaatt tttcttttaa tacttcaacc ggtgcatgtg
     1981 tatttgtaaa tgcctgaaaa taagcaatat attttccttc gtgccatttc tcatgcatct
     2041 tttctttaat ttctttaaat tgtactgcga ttgaatctgc acgattacct gcaaagtctc
     2101 cgctacctgc agcagaacaa aatgtacatc caccatgtgc tacagtgcca tcgcggttag
     2161 gacagtcaaa cccgccatcc aatgcaactt taaatatttt ttgtccaaat ttatttttta
     2221 aatggtaatt ccatgtgtga taacgtttgt tttcaaaagc gtattggaaa tgattgccca
     2281 tatgtcattt tcctttctat aaaaaaagag ttctaagtac agattttaac atattttaat
     2341 gttatagtgt ttattatagt ttgacaaaaa agagagagga actatgaaat atgaatatac
     2401 ctaaatcagt ctggtggcta gtaattggca tggcgttaaa tattactggt tccagttttt
     2461 tgtggccttt aaatacaatt tatatgaaac aagaacttgg aaaaagttta actgttgctg
     2521 gtttagtgct aatgataaat tcatttggca tggttattgg aaacttatta ggtggttcac
     2581 tatttgataa attaggtgga tacaagacga ttttaattgg aactttcact tgtctttgta
     2641 gtacaacgct acttaatttc tttcacgggt ggccttggta tgctgtatgg cttgtaatgt
     2701 tagggtttgg tggcggaatg attattcctg cgatatacgc tatggctgga gcagtgtggc
     2761 caaatggcgg aagacaaacg tttaatgcga tatacttagc gcaaaatatt ggtgtggctg
     2821 tcggtgctgc aatgggcggc tttgtcgcag aatttagctt taactatatc tttttagcca
     2881 atcttattat gtatgttgtg tttgcgcttg tcgcggtaac gcaatttaat attgaaatta
     2941 atgcgaaagt taaatatcca actcatttag atattactgg taaaaagaat aaagcaagat
     3001 ttatttcatt agtactaatt tgtgcaatgt ttgcaatttg ttgggttgca tatattcaat
     3061 gggagtctac aatcgcttca tttacacaat ctattaatat ttcaatggca caatatagtg
     3121 ttttatggac aattaacgga ataatgattt tagtagcaca accattaatt aaaccgattc
     3181 tctatctgtt aaaaggaaac ttaaagaagc aaatgtttgt cggcatcatc atttttatgt
     3241 tgtcgttctt tgtcacgagt tttgccgaaa actttacaat atttgttgtc ggtatgatta
     3301 ttttaacttt tggagaaatg tttgtatggc cagcagttcc aactatagcc aatcagttag
     3361 cgccagatgg taagcaagga cagtaccaag gttttgtgaa ttcagctgct acagtaggaa
     3421 aagcatttgg tccatttctt ggtggtgtat tagttgatgc gtttaatatg cgcatgatgt
     3481 ttatcggtat gatgctacta cttgtatttg cattaatatt attaatggtt ttcaaggaga
     3541 ataatacgca acctaaaaaa atagatgcat aatgagtaaa tagaattaac gttatagact
     3601 tgaaataaat gtcgttataa cataatatta atttgtataa tttaatttcg tttggagctt
     3661 ttctacagaa agctagtgat gctgagagct agtgttaagg actaaatgta aatcgtatta
     3721 attttaaatt gaatgaatga catctcttac tattaaaatg agtgcacaat ttttgtgaaa
     3781 tagggtggta acgcggcaaa tgtcgtccct atgtaaatag aatagttaga ggtgtctttt
     3841 ttattgaata ggaggaaatg tgttgaatta taaccacaat caaattgaaa agaaatggca
     3901 agactattgg gacgaaaata aaacatttaa aacaaatgat aacttaggtc aaaagaaatt
     3961 ttatgcttta gacatgtttc catatccatc aggtgctggt ttacatgttg gacatcctga
     4021 gggctataca gcaacagata tcatttcaag atataaaaga atgcaaggat ataatgtatt
     4081 acatccgatg gggtgggatg cattcggatt accagcagag caatatgctt tagacactgg
     4141 caacgaccca cgtgaattta caaagaaaaa tatccaaact tttaaacgac aaattaaaga
     4201 attagggttc agttatgatt gggatcgtga agttaataca acagatccag aatactataa
     4261 atggacacag tggattttca tacagttata taacaaaggt ttagcatacg ttgatgaagt
     4321 tgcagttaac tggtgtccag cattaggcac tgttttatct aacgaagaag tgattgatgg
     4381 tgtctctgaa cgtggtggac atccagttta tcgtaagccg atgaaacaat gggtacttaa
     4441 aatcacagaa tatgcagatc aattattagc agatttagat gatttagatt ggcctgagtc
     4501 tttaaaagat atgcagcgca attggattgg acgttctgaa ggggccaaag tttcatttga
     4561 tgtagataat acggaaggaa aagtagaagt atttacgact agaccagata caatctatgg
     4621 tgcatcattc ttagtcttaa gtcctgaaca tgcattagtt aattcaatta caacagatga
     4681 atataaagaa aaagtaaaag cttatcaaac agaagcttct aaaaagtcag atttagaacg
     4741 tacagattta gcaaaagata aatcaggtgt atttactggt gcatatgcaa ctaatccttt
     4801 atctggtgaa aaagtacaaa tttggattgc tgattatgta ttatcaacat atggtactgg
     4861 agcaattatg gcagtaccag cgcatgatga cagagattat gaatttgcta aaaagtttga
     4921 tttgccaatc attgaagtca tcgaaggtgg aaatgttgaa gaagcagcat acactggtga
     4981 aggtaaacat attaattctg gtgaacttga tggtttagaa aatgaagcgg caattactaa
     5041 agctattcaa ttattagagc aaaaaggtgc tggcgaaaag aaagttaatt acaaattaag
     5101 agattggtta ttcagtcgtc agcgttattg gggcgaacca attcctgtca ttcattggga
     5161 agatggaaca atgacaactg ttcctgaaga agagctacca ttgttgttac ctgaaacaga
     5221 tgaaatcaag ccatcaggga ctggtgagtc tccactagct aatattgatt catttgtaaa
     5281 tgttgtagat gaaaaaacag gtatgaaagg acgtcgtgaa acaaatacaa tgccacaatg
     5341 ggcaggtagt tgttggtatt atttacgtta catcgatcct aaaaatgaaa atatgttagc
     5401 agatcctgaa aaattaaaac attggttacc tgttgattta tatatcggtg gagtagaaca
     5461 tgcggttctt cacttattat atgcaagatt ttggcataaa gtcctttatg atttggctat
     5521 cgtacctact aaagaacctt tccaaaaatt atttaaccaa ggtatgattt taggagaagg
     5581 taatgagaag atgagtaaat ctaaaggaaa tgtaatcaat cctgatgata tagtacagtc
     5641 tcatggtgca gatactttgc gtctttacga aatgtttatg ggacctttag atgctgcaat
     5701 tgcatggagt gaaaaaggat tagatgggtc tcgtcgattc ttagatcgcg tatggcgttt
     5761 aatggtaaat gaagatggga cattgagttc aaaaattgta actacaaata ataaatcttt
     5821 agataaagtt tataaccaaa ctgttaaaaa ggtaacagaa gactttgaaa cattaggatt
     5881 taatactgct attagtcaat taatggtatt tattaatgag tgttataaag ttgatgaagt
     5941 ttataaacct tacattgaag gcttcgttaa aatgttagca cctattgcac cacatatcgg
     6001 tgaagaatta tggtcaaaat taggacatga agagtctatt acgtaccaac cttggccaac
     6061 ttatgacgaa gcactacttg tagatgatga agtagaaatc gttgttcaag tgaatggtaa
     6121 attgagagct aaaattaaaa ttgctaaaga tacatcaaaa gaagaaatgc aagaaattgc
     6181 cttatctaat gacaatgtta aagcgagtat tgaaggtaaa gacatcatga aagtcatcgc
     6241 tgttcctcaa aaattagtca atattgtagc taaataatgt tttaaggagg actttgaaat
     6301 gaagtcaatt actacagatg aattaaaaaa taaactttta gaatctaaac cagttcaaat
     6361 tgttgatgtt cgtactgatg aagaaacagc aatgggatat attcctaatg caaagttaat
     6421 tccaatggat accattccgg ataatttaaa ttcatttaat aaaaatgaaa tatattatat
     6481 tgtatgtgct ggtggagttc gaagcgctaa agttgtagaa tatttagagg caaatggcat
     6541 tgatgccgta aatgtcgaag gcggcatgca cgcatggggc gatgaaggtt tggaaataaa
     6601 aagtatttaa agtagtgaca taatttaaaa taatattaca tttgtaatga caccaagtaa
     6661 cgtttcggtt gcttggtgtt ttttggtatg aattactttc tgttacaaaa caatctaaag
     6721 cgttcttgtt atgttttatt aagattttaa ttacaaaacg gaaactaaat tgtaataaaa
     6781 taaaacttta ttttataaaa tgatgatgat aaaattgagt gaacttaaaa tattgtacaa
     6841 aataatatag ctataaatat aatatgaggg agcgtatatt tttagcataa ttcttaacaa
     6901 cacagcagag aacagacaac caggaggaaa atgaaatgaa tttgttaaag aaaaataaat
     6961 atagtattag gaagtataaa gtaggcatat tctctacttt aatcggaaca gttttattac
     7021 tttcaaaccc aaatggtgca caagccttaa ctacggataa taatgtacaa agcgatacta
     7081 atcaagcaac acctgtaaat tcacaagata aagatgttgc taataataga ggtttagcaa
     7141 atagtgcgca gaatacacct aatcaatctg caacaaccaa tcaagcaacg aatcaagcat
     7201 tggttaatca taataatggt agtatagtaa atcaagctac gccaacatca gtgcaatcaa
     7261 gtacgccttc agcacaaaac aataatcata cagatggcaa tacaacagca actgagacag
     7321 tgtcaaacgc taataataat gatgtagtgt cgaataatac cgcattaaat gtaccaacta
     7381 aaacaaatga aaatggttca ggaggacatc taactttaaa ggaaattcaa gaagatgttc
     7441 gtcattcttc aaataaacca gagctagttg caattgctga accagcatct aatagaccga
     7501 aaaagagaag tagacgtgcg gcaccggcag atcctaatgc aactccagca gatccagcgg
     7561 ctgcagcggt aggaaacggt ggtgcaccag ttgcaattac agcgccatat acgccaacaa
     7621 ctgatcctaa tgccaataat gcaggacaaa atgcacctaa cgaagtgctg tcatttgatg
     7681 acaatggtat tagaccaagt accaaccgtt ctgtgccaac agtaaacgtt gttaataact
     7741 tgccgggctt cacactaatc aatggtggca aagtaggggt gtttagtcat gcaatggtaa
     7801 gaacgagcat gtttgattca ggagataata agaactatca agcacaagga aatgtaattg
     7861 cattaggtcg tatacatgga actgatacga atgaccatgg cgattttaat ggtatcgaga
     7921 aagcattaac agtaaatccg aattctgaat taatctttga atttaataca atgactacta
     7981 aaaacggtca aggcgcaaca aatgttatta tcaaaaatgc tgatactaat gatacgattg
     8041 ctgaaaagac tgttgaaggc ggtccaactt tgcgtttatt taaagtacct gataatgtga
     8101 gaaatctcaa aattcaattt gtacctaaaa atgacgcaat aacagatgcg cgtggcattt
     8161 atcaactaaa agatggttac aaatactata gctttgttga ctctatcgga cttcattctg
     8221 ggtcacatgt ttttgttgaa agacgaacaa tggatccaac agcaacaaat aataaagagt
     8281 ttactgtaac aacatcatta aagaataatg gtaattctgg tgcttctcta gatacaaatg
     8341 actttgtata tcaagttcaa ttacctgaag gtgttgaata tgtgaacaat tcattgacta
     8401 aagattttcc aagtaacaat tcaggcgttg atgttaatga tatgaatgtt acatatgatg
     8461 cagcaaatcg tgtgataaca attaaaagta ctggaggagg tacagcaaac tctccggcac
     8521 gacttatgcc tgataaaata ctcgatttaa gatataaatt acgtgtaaat aatgtgccga
     8581 caccaagaac agtaacattt aacgagacat taacgtataa aacatataca caagatttca
     8641 ttaattcagc tgcagaaagt catactgtaa gtacaaatcc atatactatc gatatcatca
     8701 tgaataaaga tgcattacaa gccgaagttg acagacgtat tcaacaagct gattatacat
     8761 ttgcgtcatt agatatcttt aatggtctga aacgacgcgc acaaacgatt ttagatgaaa
     8821 atcgtaacaa tgtaccatta aataaaagag tttctcaagc atatattgat tcattaacta
     8881 atcaaatgca acatacgtta attcgaagtg ttgatgctga aaatgcagtt aataaaaaag
     8941 ttgaccaaat ggaagattta gttaatcaaa atgatgaatt gacagatgaa gaaaaacaag
     9001 cagcaataca agttatcgag gaacataaaa atgaaataat tggtaatatt ggtgaccaaa
     9061 cgactgatga tggcgttact agaatcaaag atcaaggtat acagacctta agtggggata
     9121 ctgcaacacc ggttgttaaa ccaaatgcta aaaaagcaat acgtgataaa gcaacgaaac
     9181 aaagggaaat tatcaatgca acaccagatg ttactgaaga cgagattcaa gatgcactaa
     9241 atcaattagc tacggatgaa acagatgcta ttgataatgt tacgaatgct actacaaatg
     9301 ctgacgttga aacagctaaa aataatggca tcaatactat tggagcagtt gttcctcaag
     9361 taactcataa aaaagctgca agagatgcaa ttaaccaagc aacagcaacg aaaagacaac
     9421 aaataaatag taatagagaa gcaactcagg aagagaaaaa tgcagcattg aacgaattaa
     9481 ctcaagcaac caaccatgct ttagaacaaa tcaatcaagc aacaacaaat gctgatgttg
     9541 ataacgccaa aggagatggt ctaaatgcca ttaatccaat tgctcctgta actgttgtta
     9601 agcaagctgc aagggatgcc gtatcacatg atgcacaaca acatatcgca gagatcaatg
     9661 ctaatcctga tgcgactcaa gaagaaagac aagcagcaat tgacaaagtg aatgctgctg
     9721 taactgcagc aaacacaaac attttaaacg ctaataccaa tgctgatgtt gaacaagtaa
     9781 agacaaatgc gattcaagga atacaagcaa ttacaccagc tacaaaagta aaaacagatg
     9841 caaaaaatgc catcgataaa agtgcggaaa cgcaacataa tacgatattt aataataatg
     9901 atgcgacgct cgaagaacaa caagcagcac aacaattact tgatcaagct gtagccacag
     9961 cgaagcaaaa tattaatgca gcagatacga atcaagaagt tgcacaagca aaagatcagg
    10021 gcacacaaaa tatagtagtg attcaaccgg caacacaagt taaaacggat actcgcaatg
    10081 ttgtaaatga taaagcgcga gaggcgataa caaatatcaa tgctacaact ggcgcgactc
    10141 gagaagagaa acaagaagcg ataaatcgtg tcaatacact taaaaataga gcattaactg
    10201 atattggtgt gacgtctact actgcgatgg tcaatagtat tagagacgat gcagtcaatc
    10261 aaatcggcgc agttcaaccg catgtaacga agaaacaaac tgctacaggt gtattaaatg
    10321 atttagcaac tgctaaaaag caagaaatta atcaaaacac aaatgcaaca actgaagaaa
    10381 agcaagtggc tttaaatcaa gtggatcaag agttagcaac ggcaattaat aatataaatc
    10441 aagctgatac aaatgcggaa gtagatcaag cgcaacaatt aggtacaaaa gcaattaatg
    10501 cgattcagcc aaatattgtt aaaaaacctg cagcattagc acaaatcaat cagcattata
    10561 atgctaaatt agctgaaatc aatgctacac cagatgcaac gaatgatgag aaaaatgctg
    10621 cgatcaatac tttaaatcaa gacagacaac aagctattga aagtattaaa caagctaaca
    10681 caaatgcaga agtagaccaa gctgcgacag tagcagagaa taatatcgat gctgttcaag
    10741 ttgatgtagt aaaaaaacaa gcagcgcgag ataaaatcac tgctgaagtg gcgaagcgta
    10801 ttgaagcggt taaacaaaca cctaatgcaa ctgacgaaga aaagcaggct gctgttaatc
    10861 aaatcaatca acttaaagat caagcaatta atcaaattaa tcaaaaccaa acaaatgatc
    10921 aggtagacac aactacaaat caagcggtaa atgctataga taatgttgaa gctgaagtag
    10981 taattaaacc aaaggcaatt gcagatattg aaaaagctgt taaagaaaag caacagcaaa
    11041 ttgataatag tcttgattca acagataatg agaaagaagt tgcttcacaa gcattagcta
    11101 aagaaaaaga aaaagcactt gcagctattg accaagctca aacgaatagt caggtgaatc
    11161 aagcagcaac aaatggtgta tcagcgatta aaattattca acctgaaaca aaagttaaac
    11221 cagctgcacg tgaaaaaatc aatcaaaaag cgaatgaatt acgtgctaag attaatcagg
    11281 ataaagaagc aacagcagaa gaaagacaag tagcactaga taaaatcaat gaatttgtaa
    11341 atcaagccat gacagatatt acgaataata gaacaaatca acaagttgat gatacaacaa
    11401 gtcaagcgct tgatagcatt gctttagtga cgcctgacca tattgttaga gcagctgcta
    11461 gagatgcagt taagcaacaa tatgaagcta aaaagcgcga aattgagcaa gcggaacatg
    11521 cgactgatga agaaaaacaa gttgctttaa atcaattagc gaataatgaa aaacgtgcat
    11581 tacaaaacat cgatcaagca atagcgaata atgatgtgaa acgtgttgaa acaaatggca
    11641 ttgctacact aaaaggtgta caacctcata ttgtaattaa gcctgaagca caacaagcaa
    11701 taaaagcaag tgcagaaaat caagtagaat caataaaaga tacaccacat gcaacagttg
    11761 atgaattaga tgaagcgaat caattaatta gcgacacact caaacaagcg caacaagaaa
    11821 tagaaaatac aaatcaagat gctgctgtta ctgatgttag aaatcaaaca atcaaggcaa
    11881 tagagcaaat aaaacctaaa gtaagacgta aacgagctgc gcttgatagc attgaagaaa
    11941 ataataaaaa tcaactcgat gcaatccgaa atacgttgga tactactcaa gatgaaagag
    12001 atgttgctat tgatacttta aataaaattg taaatacaat taaaaatgac attgcacaaa
    12061 acaaaacgaa tgcagaagtg gatcgaactg agactgatgg caacgacaac atcaaagtga
    12121 ttttacctaa agttcaagtt aaaccagcag cgcgtcaatc tgttggtgta aaagccgaag
    12181 ctcaaaatgc actaatcgat caaagcgatt tatcaactga agaagaaaga ctagctgcta
    12241 aacatttagt agaacaagca cttaatcagg ctattgatca gatcaatcat gcagataaga
    12301 ctgcccaagt taatcaagat agtataaatg ctcaaaatat tatttcaaaa attaaaccag
    12361 cgacaacagt taaagcaaca gcattacaac aaattcaaaa tatcgctaca aataaaatta
    12421 atttaattaa agcaaataac gaagcgacag atgaagaaca aaatattgca atagcacaag
    12481 ttgaaaaaga gttaattaaa gctaaacaac aaattgctag tgcagtgact aatgcagatg
    12541 tggcatattt attgcatgat gagaaaaacg aaattcgtga aatcgaacct gttattaaca
    12601 gaaaggcgtc tgctcgagaa caattgacaa cattattcaa cgataaaaaa caagcaattg
    12661 aagcgaatat tcaagcaacg gtagaagaaa gaaatagtat attagcacag ttacaaaata
    12721 tttatgacac tgctattgga caaattgatc aagatcgtag caatgcacaa gttgataaaa
    12781 cagcatcatt aaatctacaa acaatacatg atttagatgt acatcctatt aaaaagccag
    12841 atgctgaaaa aacgattaat gatgatcttg cacgcgtcac tgctttagtg caaaattatc
    12901 gaaaagtaag taatcgtaat aaggctgatg cattaaaagc tataactgct ttaaaattac
    12961 aaatggatga agaattaaaa acagcacgca ctaatgctga tgttgatgca gttttaaaac
    13021 gatttaatgt tgcattaagc gatatagaag cagtaattac tgaaaaagaa aatagcttac
    13081 tgcgaattga taacattgct caacaaacat atgcgaaatt caaagcgatc gcaacaccag
    13141 aacaattagc taaagtaaaa gtattaattg atcaatatgt tgcagatggc aatagaatga
    13201 ttgatgaaga tgcgacatta aatgacatca aacaacacac gcaattcatt gttgatgaaa
    13261 ttttagcaat taaattacca gctgaagcga cgaaagtatc accaaaagaa attcagccag
    13321 ctccaaaagt ttgtacgcct attaaaaaag aagagacaca tgaatcgcgc aaagttgaaa
    13381 aagaacttcc aaatacaggt tctgaaggaa tggatttacc attgaaagaa tttgcactga
    13441 ttacaggtgc ggctttgtta gctagaagac gtactaaaaa cgaaaaagaa tcataattaa
    13501 cgaaatgacc ttaatataac gacacttttt agtgactcag ttatattaag gtcattttta
    13561 tttgaatgaa aaagttgatc tactaaagag aatcgaacca aattttatac tattattcca
    13621 ttgatgcatg tgagtaatgt ccggcatata atccagcgac atgtcctgtt acgagtgcac
    13681 ttgtaatatt ataaccacca gtataaccat gtatatctaa tacttcacca cataaaaata
    13741 atcccggaac taatttagac atcattgttt taggttgaat ttctttaagt gacacaccac
    13801 cacctgtgac aaatgcctta tctataggta atgtcccgtt caccttaaat acaaaccctt
    13861 taaacatatt tactaagtcg ttcaattgtt gatttgataa gtgatgtgat gtggtatttt
    13921 catcgattcc tgcttgttcc agcatgaaca gtaagtagcg ctcttcaatt agaccatgca
    13981 aactgttttt aatgatttta tctggtgtgt ccgataataa tgatgtgatg tgttgtttta
    14041 attgttcatg gtttaattca ggaaatgcat cgattgccat agaaatgtgc tgtgtctttt
    14101 gatttttttg ttctttataa acaaactgac tacatcttaa tgcagctgga ccactgatac
    14161 caaaatgagt aaataacata tccatttgat gactgatgtg ttttttacca tttttcttaa
    14221 gtactgacaa ttcaacatct tttaaactta aaccttttag acgattggat ttgatgaaag
    14281 gttcagctga tgtaattgga acttcggtcg ggaataactc agtaatggta tgacctaaat
    14341 cttgtgcgaa cttataacca tcaccagttg aaccagtttg agggacactt gtaccacctg
    14401 tagcaatcac tagcgaatgg ctttcataac tattattttg agtatgtaca gtgaaagttt
    14461 ggtctgtatt aacttcgatt ctactaacag cttcttcttc tttaatcgtt acatgttggc
    14521 gttcgatagt tgtcactaat gtatcaacca cgtcttgtgc tttgttggaa actggaaaca
    14581 tacgcccgtg atcttcttct tttaatttaa cacccctaga ctcaaaaaaa tctatgatgg
    14641 attcattatc aaaaattgaa aagggactat ataaaaattt cccatttcca ggaatgttct
    14701 taataatttc agcatatggt aatcgattag ttacgttaca tctaccgcca ccagatattt
    14761 tgagtttacg acctagacct ttcttttttt caatgagtaa cacactgcta ctttgttcgc
    14821 ttgcagctac tgccgccatt aagccgctag gtccgcctcc gataataatt gtttgataca
    14881 tatacgcttc ctccaaattt gttactggac attcaattgc tattttatag tactttttta
    14941 aactttgcta tatctcttgt gttatgagat gacaattatg ttgaatatat ttataatgtg
    15001 aacttcataa aaaatgaaga tgtatgtgta tgataagaat gatttttcta ctgttatgcg
    15061 atgttcatct caaatagtag tataatgttt atatgaaatt tcgtaatatt ctaatcaaaa
    15121 tctagtattc aatgacaatg tgttgataat tttgttatga taattcattg aatatacgac
    15181 aatgaaataa agttttgtga tatattcata ataatgaaaa atgattttac agagaaacag
    15241 cttttagttt acaaaacttt aataaacatg agatagggag attcgtaatg agtgaaagta
    15301 aagaaatggt gcgtggaacc tttttaatta ccattagtat attaattacc aaggtattgg
    15361 gcgtactttt tatcattcca ttcaactatt taattggtgg acaagaaaat atggcgccgt
    15421 tcacatacgc ttatgcacca tataatattg caattgcagt tgcgacagca ggtgtaccat
    15481 tagcagcttc gaaatatgtt gcgaaatata atgctattgg ggcatataaa gtaagtcaga
    15541 aattttataa atcgagtttt attgtaatga gtattaccgg tgttttaggt ttcttggtac
    15601 tgtatttctt agcaccttat atttctgaat taacattagc tagaaacatt catgataaaa
    15661 acggttggtc agtcgatgat attacttgga ttataagaat cattagtatg gttgttatct
    15721 ttattcctgt attagcgaca tggagaggga tattccaagg ttataaatct atggggccaa
    15781 cagcagtgtc agaagtaact gagcaaattg cacgcgtgat tttcatatta atcggaagtt
    15841 atttagtact aaatgtcttt gatggctcta ttttattagc caatggtatt gctacttttg
    15901 cagcagcagt tggtgcgatt attggtatat tcacactctg gtattactgg agaaagcgta
    15961 agcataatat tgatcgtatg gtcgaatctg attacacaga tatagatgtg tcatatggca
    16021 agatgtataa agaaattatt gcttatagta taccgttcgt tattgtaagt ttgaattatc
    16081 cattatttaa cttagtagat caatttacac ataacggtgc tttatcttta gttggtatac
    16141 cttcacagtt acaagatata ttctttaata tgctaaatat gtcaactaat aaaattgtaa
    16201 tgattccgac atcattaagt gctggttttg cagtgagttt aattccttat attacaaaaa
    16261 catttgcaga aggtcgctta catgaaatgc atcatcaaat tagaacatct attggtgtat
    16321 taatgtttat aactgttcca gctagtattg gaattatggc cttggcacaa ccattattca
    16381 cagttttcta tggttacgat ccaattgttt taggtcatga ccctaaccat gatggtagtc
    16441 gcttattatt ctattatgcg cctgtagcaa tactaatttc actattaagt gtaacggcat
    16501 caatgttaca aggtatagat aaacagaaat taacagttta tgttatttta gcttctgtag
    16561 ttatcaagtt agcgttaaac tatccattaa ttatgttatt ccatacacct ggtgcaattt
    16621 taagtactag tatcgcatta ttatttgcaa ttggatgtaa tttctatatt cttaaaaaat
    16681 atgcgaagtt taaattcagc tatagttgga ttcattttgc aaaaatattc ttgtattcat
    16741 ttattatgat gctaggtgta gaattagtat ttttccttgc aaatctattc ttagaaccta
    16801 ctaaacttgg ttacttaatc attattattt taggtgttac agtaggtatt ctaatttacg
    16861 gtacaatcac aattaaaacg cgtcttgcgg acgaattctt aggtgaaatt cctgaaaaat
    16921 taagacgtag agttaggttt ttacgatgag aatagataaa tttttagcaa atatgggcgt
    16981 cggtacacgt aatgaggtca aacagttact taaaaaaggc cttgtaaatg tcaacgaaca
    17041 agtaattaaa tcaccaaaaa cacatataga accagaaaac gataagataa cagttcgtgg
    17101 agaattaata gaatacattg aaaatgtcta tattatgtta aataagccaa aaggttatat
    17161 atcagcaact gaagatcatc attcaaaaac cgttattgat ttaatacctg aataccaaca
    17221 tctaaatatt tttccggttg gtcgtcttga taaagataca gaagggcttt tattgataac
    17281 gaatgatggt gattttaatc atgaattaat gagtccaaac aaacatgttt ctaaaaaata
    17341 tgaagttatt tcagcaaatc ctatcactga agacgacatt caagctttta aagagggtgt
    17401 aacactgact gatggcaagg ttaagcctgc aatattaact tatattgata atcaaacgtc
    17461 acacgtaaca atttatgaag gaaaatatca tcaagttaaa cgaatgtttc atagcattca
    17521 aaatgaggta ttacatttaa gacgtatcaa aattgctgat ttagaattgg acagcaattt
    17581 agattcagga gaatatcgtt tattaactga aaatgatttt gacaaattaa attataaata
    17641 aaagggagac gattgatatg gcaaaaggta atttatttaa agcgatttta ggtataggtg
    17701 gcgctgtagc agctgtactt gttacacgta aagatagtcg tgacaagctg aaagcagaat
    17761 ataataaata taaacaagat cctcaaagct ataaagataa tgctaaggat aaagcgacgc
    17821 aattaggaac aattgctaat gaaacaatta aagaagtaaa aacaaatccg aaagaatatg
    17881 ctaatagatt aaaaaataat ccaaaagcat ttttcaaaga agaaaaatca aaatttaccg
    17941 aatatgacaa taagactgac gaaagtattg aaaaaggtaa atttgatgat gaaggtggcg
    18001 cagcaccaaa taataattta cgtatcgtca ctgaagaaga tttaaaaaag aataaaaatg
    18061 cattatctga taaagaataa gatgtgagta ggacagaaat gataagggag tgggacagaa
    18121 atcatatttt agcaaaattt atttcgtcgt cccaccccgg caaggttgac tagaattgaa
    18181 aaaagcttgt tacaagcgta ttttcattca gtcaactact gccaatataa tattgtaaac
    18241 tataggacat ttattagtgt ttcaggttct ttatcgttag tcatagctaa tatgcatata
    18301 gaaagaagaa tacatgcgtg aaactcattc ataagaaata ctaatatcta aagaaaacgt
    18361 atttctttat cttttcgaaa ttctctgtgt tggggccctt accccaactt gcacattatt
    18421 gaaagctgac tattggccag cttctgtgtt ggggccctta ccccaacttg cacattattg
    18481 aaagctaact attggccagc ttctatgttg gggccccgcc aacttgcatt gtctgtagaa
    18541 tttcttttcg aaattctcta tgttggggcc ccggggcgca ttttcgttcg gttaattact
    18601 gtcaatatta aattgtgtgc ctagtacatt gacttatatc tcgagctctt gaatatgtgt
    18661 attttaaata tataaattaa aaaaggaaca aagcagctat ttatgatttg tgtcatatca
    18721 atagcatcgt tttgttcctt tttattattt tctgacatct tattgtagtt tttatatctt
    18781 ttcatgtaaa atagaattta acttatagaa aaaaggaagt cgatttcgaa tgtggaaaga
    18841 aaaagttcaa caatacgaag atcaaatcat taatgactta aaaggattat tagcaattga
    18901 aagtgtgaga gatgatgcaa aagcatcaga agacgcacca gttggtccag gtcctcgtaa
    18961 ggcattagac tacatgtatg aaattgcaca tagagatgga tttacaacac atgatgtgga
    19021 tcatattgca ggaagaattg aggcaggtaa aggaaatgac gtattaggta tcttatgtca
    19081 tgttgacgtt gttcctgctg gtgatggatg ggatagtaat ccgttcgagc cggttgtaac
    19141 agaagatgct atcatagcta gaggtacact tgatgacaaa ggtccaacaa ttgctgctta
    19201 ttatgcaatt aagatattag aagatatgaa tgtggattgg aagaaacgta ttcatatgat
    19261 tattggtacg gatgaagaat ctgattggaa atgtacggat cgctatttta aaacagaaga
    19321 aatgccaaca ttaggttttg caccagatgc agaatttcca tgtattcatg gtgaaaaagg
    19381 cattacaaca tttgatttag ttcaaaataa acttactgaa gatcaagatg aacctgatta
    19441 tgaattaata acttttaaat ctggtgaacg ttacaacatg gtacctgatc atgcagaagc
    19501 aagagtgctt gttaaagaaa atatgacaga tgttattcaa gactttgagt actttttaga
    19561 acaaaatcat ttacaaggtg atagtactgt tgatagtggc attctagttt taacagttga
    19621 aggtaaagcg gttcatggta tggatccatc tatcggtgtg aatgcgggtc tttacttact
    19681 aaaattctta gcatcattaa atcttgataa taatgcacaa gcgtttgtag catttagtaa
    19741 tcgctactta tttaattcag attttggtga aaagatggga atgaaattcc atacagatgt
    19801 catgggtgac gtgacaacta acattggtgt tattacatat gataatgaaa acgcaggtct
    19861 tttcggtatc aacttacgct acccagaagg atttgaattt gaaaaagcta tggatcgttt
    19921 tgcaaatgag attcaacaat atggctttga agtgaaatta ggtaaagtcc aaccaccaca
    19981 ttatgttgat aaaaatgatc cttttgtaca aaagttagtt actgcatata gaaatcaaac
    20041 aaatgatatg actgaacctt atactatagg tggcggtact tatgcgagaa acttagacaa
    20101 gggtgtagca tttggcgcaa tgtttagtga ttctgaagat ttaatgcatc agaaaaatga
    20161 atatatcact aaaaaacagt tatttaacgc aactagtatt tacttagaag caatttattc
    20221 attatgcgtg gaggaataat atatggaaaa aattttttta aatggtgagt ttgtaagtcc
    20281 aagtgaagca aaggtttcat acaacgacag aggatacgta tttggcgatg gtatttatga
    20341 atacattcga gtatataatg gtaagttatt tacagtaaca gaacattatg aaagattttt
    20401 acgtagtgcc aatgagattg gtttagattt aaattattct gtagaagaat taattgaact
    20461 atctcgtaaa ttagttgata tgaatcaaat tgaaactggg gcaatttata ttcaagcaac
    20521 gcgtggtgta gctgaaagga atcatagctt cccgacacct gaagtagaac cagcaattgt
    20581 tgcttataca aagagttatg atcgtcctta tgatcattta gaaaatggtg tgaatggtgt
    20641 taccgttgaa gatatccgat ggttacgttg cgacattaaa agcttgaact tattaggaaa
    20701 tgtattagca aaagaatatg ctgtgaaata taatgcagtt gaagcaattc aacatcgagg
    20761 tgaaactgta actgaaggat cttcaagtaa tgcttatgca attaaagacg gtgtgattta
    20821 tacacatccg attaacaact atattcttaa tggtattaca cgaattgtaa ttaaaaaaat
    20881 tgccgaagac tataacatcc catttaaaga agaaacgttt actgtagatt tcttgaaaaa
    20941 cgcagatgaa gttattgttt caagtacttc agctgaggtt acacctgtta ttaaattaga
    21001 tggtgaacca gttaatgatg gtaaagttgg cccaattaca cgtcaactac aagaaggatt
    21061 tgaaaagtat atagagtcac acagtattta aaattctttc atcatatttt tagattaaat
    21121 tacttaaatt tatcaacttt cgtaagtgaa ttatgttata atttaaaacg aagcttaaaa
    21181 gtgttgttta gaaaacacat attagtaaaa agtgccattt tgtttctcat tttcagttga
    21241 aatataatga taaaaattat aaaattttct atgagttaca aatatgatgt gcatcatatt
    21301 ttactaagta cgtaaatgtt acagttcata aatttatgct aaccatgaaa atgggatagc
    21361 aattattttg aaaactagag tttatcgcct acagagattc aaaaacatgt agttttagac
    21421 acggtaacat tatatgtcaa gtaagtgact atagaatgta tggtgtcaat gacagacatt
    21481 acgatttaaa tttagtcgtg taatgtaggc attggttgag gatttacaat aattatctgg
    21541 acataatatt tgtaccagta tggtgttata aattagaata tatggctctt gaaaaaccta
    21601 tttctagagc cattttctaa ttgaaagtga ggtggacgag ttggagcagt tttatcaatt
    21661 agggtggaca cttgattcag caggtggtgc atctggtgaa gcatatatgg ctgaacaaga
    21721 tggacaaaag ttgtttttaa aacgaaattc aaatccattt attgcggcat tatcagcaga
    21781 aggtattgtg cccaaattag tatggacgaa acgcatagaa acaggcgagg ttgttacagc
    21841 acaacattgg aaaaatgggc gtgaactatc ttcaaacgaa atgaagcaaa caagagttgc
    21901 acatttatta aagaagatac acaattctag acctttatta agtatgttaa agcgtatgga
    21961 aatggaacct attactcctg agattatgct taataaaatt aatgcctctt tatcaagaga
    22021 agttttaaca catcatattg tgagaaaatc attaacctat ttagaagagc atataccgag
    22081 tttagattcg cgttttttca ctgttgtaca tggtgatgtg aatcataata attggttatt
    22141 atccgatcga gatgaactat ttttagttga ttgggaaggt gcaatgattg cagacccggc
    22201 aattgatatc ggaatgctgc tttacaacta tgttccacaa caacaatggt ctgaatggct
    22261 tgaaacatat ggtgttcaag agagcttgaa tttaaataaa cgtatgaaat ggtatacggt
    22321 aattcaatct ataggtcttg ttcaatggta tgaagagcaa aaaagatata aggatatgaa
    22381 cacttggctt aaattcctaa atgaagttat gaatagtaac atgtttattt aagaggagaa
    22441 taaaatatga gagttcgata caaaccatgg gctgaagatt atttaaaaga tcatcctgaa
    22501 ttggttgaca tggacggtca acatgcaggg aaaatgactg aatggtttga taaaacgcaa
    22561 ccgatacaca ttgaaattgg ttcgggtatg gggcagttta ttacaacatt agctgctcaa
    22621 aatcctcata ttaactatat ttcaatggaa cgtgagaaaa gtatcgttta taaagtattg
    22681 gataaagtta aagagatggg tttaacaaat ttaaagatta tttgtaatga cgccattgaa
    22741 ttaaatgaat attttaaaga cggcgaagtt tcgcgtattt atttgaactt ttcagatcca
    22801 tggccaaaaa atcgtcatgc aaaacgacgt ttaacgtatc atacgttttt agcgttgtat
    22861 caacaaatct taaatgatga aggagattta cattttaaaa ctgataacag aggactattt
    22921 gcctacagtt tagaaagtat gtctcaattt ggaatgtatt ttacaaaaat aaatctaaat
    22981 ttacatcaag aagacgatgg cagtaatatt ttaacagaat atgaaaagaa attttcggat
    23041 aaagggtcac gtatttatcg tatggaagcg aaattccatt cacaaaaata gagcacttat
    23101 gagggtatgt acacatacaa tcataagtgt tttttcgtac tataatggtt ctatagataa
    23161 aggttctata ataaatcgga ctgatggaaa aagtttttaa cttttcatct gtccgatttt
    23221 ttgattttga atataaaaaa agcgcaatta tctctataat taaaagtgac taaacaaata
    23281 actaaaggag ataatgcgcc tatgaacaat gttatactaa atacctttga tttaaaagaa
    23341 taaagcttgc attttacttg taaattttaa ttattaattt aaaaatacag gccgatggtt
    23401 atgatgcatt tacatataat atgattgcat taatctttat aagtagttag aagtgtatta
    23461 gtgtggggcg tttcgttttt gttcgcattt atgaatagtg atatgatgta ataaacacat
    23521 gctaattgta ataacgaata cagaaagaat aggtgatgtt atgaaaatcg gggatatatc
    23581 tattcattat ctaaatggtg gcaatacaaa aatggatggc ggtgcaatgt ttggtgttgt
    23641 tccgaagccg ttgtggtcaa agcaatacaa tgcaaatgaa cgaaatcaaa tcaatttacc
    23701 gacacatcca attttgattc aaacggctca atataatttg attatagatg caggtattgg
    23761 taatggtaaa ttatctgaaa agcaattacg taattttgga gtagatgaag aaagtcatat
    23821 aattgctgat ttggcaaatt ataatttaac gccaaaggat attgattatg tgctaatgac
    23881 acatatgcat tttgatcatg ctgccggttt gactgatcaa gcaggacatg caatttttga
    23941 aaatgcgatt catgttgtgc aacaagatga gtggcatgag tttattgcac ctaatataag
    24001 aagtaaatca acttactggg ataagaataa aggcgattat agtaacaagt tgattttatt
    24061 cgaaaaacat tttgaaccgg ttctgggtat caagatgcaa catagtggag gtcatagctt
    24121 tggccacacg attattacga ttgaaagtca aggagataaa gcagttcata tgggtgatat
    24181 attcccaact actgcacata aaaatcctct atgggtaacg gcatatgatg attatcctat
    24241 gcaatcgatt cgtgaaaaag aacgcatgat accatatttt attcagcaac aatattggtt
    24301 cttgttttat catgatgaaa actactttgc tgtaaaatac agcgataatg gtgaaaacat
    24361 agatgcatat attttacgtg aaacattagt tgataataac taaaataaag atgtattact
    24421 aaacaaattt tcaaaaataa aaaattgagc cacatccaat cttactaatt agggtgtggc
    24481 tcatttttaa gttttacgat ccaaatcaaa tatggataaa attcgtatta acgctctaca
    24541 atgttaatga cttcaccagt atatgcatct gcataaaaat cataatgaat attttgacca
    24601 tttttaatag ttgtaattcc accttgataa actaaacggt atttatcagt ttcaggatga
    24661 acgaatggtt cataaacgat atatgagcct ttgacattca taaaatatga ttttacttca
    24721 ttaagtactt taacgggatt atagcgttta cgattaataa attggatgat tgaaatggta
    24781 gaaataatta caatggctac tgcaattatt gttggaatta tatatttcag tttagtcatc
    24841 atttatgctc ccttgatttc aaattcatat cattagttta ccatattgaa gatgatataa
    24901 taacttataa ggagtgagaa cttttatgaa cataaataaa aaagtaacat tacaacgaat
    24961 tcaaacttta actgagcttc atggggcacc aggttttgaa gaagaagtaa aaaattatat
    25021 gactcagcaa atggcgccgt acgtagatga atttattgaa aatcgtatgg gtggattttt
    25081 tggtgtgaaa aaatctaaaa atccaaatgc aaaacgtgta atgattgcag cacatatgga
    25141 tgaaatcgga tttatgatta caaatatcac taaaaatgga atgattcaat tcacaaattt
    25201 aggtggtgtt gcaaatgata tttggcaagg acaacgctta gtaattaaaa atagaaatgg
    25261 cgataaaatt atcggtgttg tttctaatat acctaaacat tttcgtactg gtagtgaagg
    25321 tgcaccggaa attaaagatt taacattaga tataggtgct caaaatgaag atgaggtgcg
    25381 tgagcgcgga atagatatag gagatacaat tgtacctcac acgccattca cacagttatc
    25441 tgaacatcga tatagtgcta aagcatggga taatcgttat ggttgtgtct tggcaattga
    25501 aatactagaa ttattaaaag atatagaatt agatgtagac ttgtatgttg gcgcaaatgt
    25561 tcaagaagag gttggattac gaggtgcgaa agcatctgca gagatgatag acccagacgt
    25621 tgcatttgta gttgattgtt cacctgccaa tgacgttaaa ggaaaccaac cattatctgg
    25681 tgaacttggt aaagggacgt taattcgcat aaaagacggt acaatgattt taaagcctgt
    25741 atttagagac tatttattaa agttagtaga agcacatgac attgaacatc aatactatat
    25801 gtcaccaggt ggaacagatg gtggagaaat tcataaagct aatattggta ttccgactgc
    25861 agttattggt gtatgtgcac gatatattca tagtacagac tcagtatttg atataagaga
    25921 ctattttgca gctagatctt tactttcaga agccatttgt aatttagata ataatcaaat
    25981 agaaacatta caatataaat aatcgggtaa taacaactat tatctctaaa tagttatata
    26041 taatcattaa ttaaggagac ataaaaatga aacaacttga atcagaacaa caatttgaat
    26101 ctttaaaaca aggtgctaca gtatttgaat tcactgcagg ctggtgtcca gattgtagag
    26161 tgatagaacc agatttaccg gaattagaag cgagatatcc tatgtttgac ttcgtatcag
    26221 tagaccgtga taaatttatg gatatttgta ttgaaaatgg tattatgggt attccaagtt
    26281 ttctagtata taaaaatgga gaactgcttg gaagttatat tggaaaagaa cgaaaatcaa
    26341 ttgaacagat agatgcattt ttagctcaat acgtgtaatt tagactagag aaaaacgggg
    26401 taatactcgt ttttctctgt tactatgtgt tgatttattg taaactatta taaggtgcga
    26461 aattaggagt gttacatatg aatacctttc aaatgagaga taaattaaag gaacgtttaa
    26521 gccatttaga cgttgatttt aaatttaatc gtgaagaaga aactttgcgt atttatcgaa
    26581 cagataataa caaaggtatc acgattaaac ttaacgctat agtcgcaaaa tatgaagata
    26641 aaaaagaaaa aattgtagat gaaattgttt attacgttga tgaagctatt gcacaaatgg
    26701 cagataaaac ccttgagagt atatcatcta gtcaaattat gcctgtcatt agagcgacta
    26761 gctttgataa aaaaactaaa caaggtgttc cttttatcta tgatgagcat actgcagaaa
    26821 cagcagttta ttatgcagtc gatttaggga aatcatatcg tctaattgac gaaagcatgt
    26881 tagaggattt gaagttaact gaacaacaaa taagagaaat gtctctgttt aatgttagaa
    26941 aattgtcaaa ttcatatacg actgatgaag taaaaggtaa tattttttat tttattaact
    27001 caaatgacgg gtatgatgca agtaggatac taaatactgc atttttaaat gaaattgagg
    27061 cacaatgtca aggcgaaatg ctcgtagcag tgccacacca agatgtgtta attattgcag
    27121 atatacgcaa taaaacagga tatgatgtga tggcacattt aacaatggaa tttttcacta
    27181 aaggtctagt tccaattaca tcattatcct ttggatataa acagggtcat cttgaaccga
    27241 tatttatttt aggtaaaaat aataaacaaa aaagagatcc aaacgtgatt cagcgtttag
    27301 aagcaaatcg tcgtaaattt aataaagata aatagaaata attggataag gagttttgtc
    27361 ataatgaatt tattttacaa tcctaaatat gtaggagatg tcgcattttt acaaattgaa
    27421 ccagttgaag gtgaattaaa ctacaataaa aaaggtaatg ttgttgaaat tactaatgaa
    27481 ggtaatgttg taggttataa tatttttgaa atttcaaaag atataacaat tgaagaaaaa
    27541 ggtcatatta aattaactga tgaacttgta aatgtattcc aaaagcgtat ttcagaagct
    27601 ggttttgatt ataaattaaa tgctgatcta tcaccgaaat ttgtagttgg ctacgttgaa
    27661 actaaagaca aacatcctga tgcagataaa ttaagtgtac taaatgtaaa cgttggaaat
    27721 gacacattac aaattgtatg tggcgcgcct aacgttgaag ctggacagaa agttgttgtt
    27781 gctaaagtag gtgcagtgat gcctagcggt atggtaatta aagatgctga attacgtggt
    27841 gttgcctcaa gcggtatgat ttgttcaatg aaagaattga atttacctaa tgcacctgaa
    27901 gaaaaaggta ttatggtatt aaatgacagc tatgaaattg gacaagcatt ttttgaataa
    27961 ttaaggaagg tagtgaaaat atgagctggt ttgataaatt attcggcgaa gataatgatt
    28021 caaatgatga cttgattcat agaaagaaaa aaagacgtca agaatcacaa aatatagata
    28081 acgatcatga ctcattactg cctcaaaata atgatattta tagtcgtccg aggggaaaat
    28141 tccgttttcc tatgagcgta gcttatgaaa atgaaaatgt tgaacaatct gcagatacta
    28201 tttcagatga aaaagaacaa taccatcgag actatcgcaa acaaagccac gattctcgtt
    28261 cacaaaaacg acatcgccgt agaagaaatc aaacaactga agaacaaaat tatagtgaaa
    28321 aacgtgggaa ttctaaaata tcacagcaaa gtataaaata taaagatcat tcacattacc
    28381 atacgaataa gccaggtaca tatgtttctg caattaatgg tattgagaag gaaacgcaca
    28441 agccaaaaac acataatatg tattctaata atacaaatca tcgtgctaaa gattcaactc
    28501 cagattatca caaagaaagt ttcaagactt cagaggtacc gtcagctatt tttggcacaa
    28561 tgaaacctaa aaagttagaa aatggtcgta tccctgtaag taaaccttca gaaaaagttg
    28621 agtcagataa acaaaaatat gataaatatg tagctaagac gcaaacgtct caaaataaac
    28681 aattagaaca agaaaaacaa aatgatagtg ttgtcaaaca aggaactgca tctaaatcat
    28741 ctgatgaaaa tgtatcatca acaacaaaat caatgcctaa ttattcaaaa gttgataata
    28801 ctatcaaaat tgaaaatatt tatgcttcac aaattgttga agaaattaga cgtgaacgag
    28861 aacgtaaagt gcttcaaaag cgtcgattta aaaaagcgtt gcaacaaaag cgtgaagaac
    28921 ataaaaacga agagcaagat gcaatacaac gtgcaattga tgaaatgtat gctaaacaag
    28981 cggaacgcta tgttggtgat agttcattaa atgatgatag tgacttaaca gataatagta
    29041 cagatgctag tcagcttcat acaaatggca tagagaatga aactgtatca aatgatgaaa
    29101 ataaacaagc gtcaatacaa aatgaagaca ctaatgacac tcatgtagat gaaagtccat
    29161 acaattatga ggaagttagt ttgaatcaag tatcgacaac aaaacaattg tcagatgatg
    29221 aagttacggt ttcgaatgta acgtctcaac atcaatcagc actacaacat aacgttgaag
    29281 taaatgataa agatgaacta aaaaatcaat ccagattaat tgctgattca gaagaagatg
    29341 gagcaacgaa taaagaagaa tattcaggaa gtcaaatcga tgatgcagaa ttttatgaat
    29401 taaatgatac agaagtagat gaggatacta cttcaaatat cgaagataat accaatagaa
    29461 acgcgtctga aatgcatgta gacgctccta aaacgcaaga gtacgcagta actgaatctc
    29521 aagtaaataa tatcgataaa acggttgata atgaaattga attagcaccg cgtcataaaa
    29581 aagatgacca aacaaactta agtgtcaact cattgaaaac gaatgatgtg aatgataatc
    29641 atgttgtgga agattcaagc atgaatgaaa tagaaaagaa taacgcagaa attacagaaa
    29701 atgtgcaaaa cgaagcagct gaaagtgaac aaaatgtcga agagaaaact attgaaaacg
    29761 taaatccaaa gaaacagact gaaaaggttt caactttaag taaaagacca tttaatgttg
    29821 tcatgacgcc atctgataaa aagcgtatga tggatcgtaa aaagcattca aaagtcaatg
    29881 tgcctgaatt aaagcctgta caaagtaagc aagctgtgag tgaaagaatg cctgcgagtc
    29941 aagccacacc atcatcaaga tctgattcac aagagtcaaa tacaaatgca tataaaacaa
    30001 ataatatgac atcaaacaat gttgagaaca atcaacttat tggtcatgca gaaacagaaa
    30061 atgattatca aaatgcacaa caatattcag agcagaaacc ttctgttgat tcaactcaaa
    30121 cggaaatatt tgaagaaagt caagatgata atcaattgga aaatgagcaa gttgatcaat
    30181 caacttcgtc ttcagtttca gaagtaagcg acataactga agaaagcgaa gaaacaacac
    30241 atccaaacaa tactagtgga caacaagata atgatgatca acaaaaagat ttacagtcat
    30301 cattttcaaa taaaaatgaa gatacagcta atgaaaatag acctcggacg aaccaacaag
    30361 atgttgcaac aaatcaagct gtacaaacat ctaagccgat gattcgtaaa ggcccaaata
    30421 ttaaattgcc aagtgtttca ttactagaag aaccacaagt tattgagtcg gacgaggact
    30481 ggattacaga taaaaagaaa gaactgaatg acgcattatt ttactttaat gtacctgcag
    30541 aagtacaaga tgtaactgaa ggtccaagtg ttacaagatt tgaattatca gttgaaaaag
    30601 gtgttaaagt ttcaagaatt acggcattac aagatgacat taaaatggca ttggcagcga
    30661 aagatattcg tatagaagcg cctattccag gaactagtcg tgttggtatt gaagttccga
    30721 accaaaatcc aacgacagtc aacttacgtt ctattattga atctccaagt tttaaaaatg
    30781 ctgaatctaa attaacagtt gcgatggggt atagaattaa taatgaacca ttacttatgg
    30841 atattgctaa aacgccacac gcactaattg caggtgcaac tggatcaggg aaatcagttt
    30901 gtatcaatag tattttgatg tctttactat ataaaaatca tcctgaggaa ttaagattat
    30961 tacttatcga tccaaaaatg gttgaattag ctccttataa tggtttgcca catttagttg
    31021 caccggtaat tacagatgtc aaagcagcta cacagagttt aaaatgggcc gtagaagaaa
    31081 tggaacgacg ttataagtta tttgcacatt accatgtacg taatataaca gcatttaaca
    31141 aaaaagcacc atatgatgaa agaatgccaa aaattgtcat tgtaattgat gagttggctg
    31201 atttaatgat gatggctccg caagaagttg aacagtctat tgctagaatt gctcaaaaag
    31261 cgagagcatg tggtattcat atgttagtag ctacgcaaag accatctgtc aatgtaatta
    31321 caggtttaat taaagccaac ataccaacaa gaattgcatt tatggtatca tcaagtgtag
    31381 attcgagaac gatattagac agtggtggag cagaacgctt gttaggatat ggcgatatgt
    31441 tatatcttgg tagcggtatg aataaaccga ttagagttca aggtacattt gtttctgatg
    31501 acgaaattga tgatgttgtt gattttatca aacaacaaag agaaccggac tatctatttg
    31561 aagaaaaaga attgttgaaa aaaacacaaa cacaatcaca agatgaatta tttgatgatg
    31621 tttgtgcatt tatggttaat gaaggacata tttcaacatc attaatccaa agacatttcc
    31681 aaattggcta taatagagca gcaagaatta tcgatcaatt agagcaactc ggttatgttt
    31741 cgagtgctaa tggttcaaaa ccaagggatg tttatgttac ggaagcagat ttaaataaag
    31801 aataattatg agtaaggagt tttatataat gacacactat cattttgtcg gaattaaagg
    31861 ttctggcatg agttcattag cacaaatcat gcatgattta ggacatgaag ttcaaggatc
    31921 ggatattgag aactacgtat ttacagaagt tgctcttaga aataagggga taaaaatatt
    31981 accatttgat gctaataaca taaaagaaga tatggtagtt atacaaggta atgcattcgc
    32041 gagtagccat gaagaaatag tacgtgcaca tcaattgaaa ttagatgttg taagttataa
    32101 tgatttttta ggacagatta ttgatcaata tacttcagta gctgtaactg gtgcacatgg
    32161 taaaacttct acaacaggtt tattatcaca tgttatgaat ggtgataaaa agacttcatt
    32221 tttaattggt gatggcacag gtatgggatt gcctgaaagt gattatttcg cttttgaggc
    32281 atgtgaatat agacgtcact ttttaagtta taaacctgat tacgcaatta tgacaaatat
    32341 tgatttcgat catcctgatt attttaaaga tattaatgat gtttttgatg cattccaaga
    32401 aatggcacat aatgttaaaa aaggtattat tgcttggggt gatgatgaac atctacgtaa
    32461 aattgaagca gatgttccaa tttattatta tggatttaaa gattcggatg acatttatgc
    32521 tcaaaatatt caaattacgg ataaaggtac tgcttttgat gtgtatgtgg atggtgagtt
    32581 ttatgatcac ttcctgtctc cacaatatgg tgaccataca gttttaaatg cattagctgt
    32641 aattgcgatt agttatttag agaagctaga tgttacaaat attaaagaag cattagaaac
    32701 gtttggtggt gttaaacgtc gtttcaatga aactacaatt gcaaatcaag ttattgtaga
    32761 tgattatgca caccatccaa gagaaattag tgctacaatt gaaacagcac gaaagaaata
    32821 tccacataaa gaagttgttg cagtatttca accacacact ttctctagaa cacaggcatt
    32881 tttaaatgaa tttgcagaaa gtttaagtaa agcagatcgt gtattcttat gtgaaatttt
    32941 tggatcaatt agagaaaata ctggcgcatt aacgatacaa gatttaattg ataaaattga
    33001 aggtgcatcg ttaattaatg aagattctat taatgtatta gaacaatttg ataatgctgt
    33061 tattttattt atgggtgcag gtgatattca aaaattacaa aatgcatatt tagataaatt
    33121 aggcatgaaa aatgcgtttt aatatgttta taatagagta gtatgggtat ttattattaa
    33181 tgacattatt acatgttaat taggaggcgt ttttaatgga ttggatttta ccaattgctg
    33241 gaattatcgc tgcgattgca ttcttaattt tatgtatcgg tatcgtagct gtattaaatt
    33301 ctgttaagaa aaacttagat tatgttgcaa aaacacttga cggtgtagaa ggtcaagttc
    33361 aaggtattac tcgtgaaaca acagatttac ttcataaagt aaaccgttta actgaggata
    33421 tccaaggtaa agtagatcgt ttaaactcag ttgtagatgc tgttaaaggt atcggtgact
    33481 cagtacaaac gttaaacagc tctgtagatc gtgtaacaaa ttcaattaca cataatattt
    33541 ctcaaaatga agataaaatc tcacaagttg ttcaatggtc aaatgttgca atggaaattg
    33601 cagacaaatg gcaaaataga cactaccgtc gtggaagtgc aaattacaaa gctaataatg
    33661 tagcaactga tgcaaatcat agctatactt ctagagtaga taaataatta ataattaaac
    33721 aaatgcactt aaatgaggtt gttacttatt taagtgcatt tgttatagga ggatatacaa
    33781 atgacgcagc aacaaaatga taaaagaaca ttaaaaaata aacacactta tcaaaatgaa
    33841 ccactaccaa accgtaaaga ttttgttgtt agttttataa ctggcgcgct tgttggttca
    33901 gctttaggtt tgtattttaa aaataaagtt tatcaaaaag cagatgattt aaaagtcaaa
    33961 gaacaagaac tgtcgcaaaa gtttgaagaa agaaaaacgc aacttgaaga aacggttgcc
    34021 tatacaaaag aacgtgttga aggattttta aacaaatcta aaaatgagca agcggcatta
    34081 aaggcacaac aagcagcgat aaaagaagaa gcaagtgcaa ataatttaag tgatacatca
    34141 caagaagcac aagagattca agaagctaaa agagaagcac aagcagaagc ggataaaagt
    34201 gtggctgtat caaataaaga atcaaaagca gtggcattga aagcacaaca agcagcgata
    34261 aaagaagaag caagtgcaaa taatttgagt gatacatcac aagaggcaca agagattcaa
    34321 gaagctaaaa aagaagcaca agcagaaaca gataaaagtg cagctgtatc aaatgaagaa
    34381 ccaaaagcag tggcattgaa agcacaacaa gcagcgataa aagaagaagc aagtgcaaat
    34441 aatttaagtg atatatcaca agaggcacaa gaggttcaag aagctaaaaa agaagcacaa
    34501 gcagagaaag acagtgacac attaactaaa gatgcaagtg cagcaaaggt agaagtatca
    34561 aaaccagagt cacaagctga aagattagca aacgctgcaa aacagaagca agctaaatta
    34621 acaccaggtt caaaagagag tcaattaact gaagcgttat ttgcagaaaa accagttgct
    34681 aaaaatgact tgaaagaaat tcctcaatta gttactaaaa agaatgatgt atcagagaca
    34741 gagacggtta atatagataa taaagacact gttaaacaaa aagaagctaa atttgaaaat
    34801 ggtgttatta cacgtaaagc tgatgaaaaa acaactaata atacagctgt tgacaagaaa
    34861 tcaggtaaac aatctaaaaa aacaacacct tcaaataaac gaaatgcatc aaaagcatct
    34921 acaaataaaa cttcaggtca gaaaaagcaa cataataaga aatcatcaca aggtgcaaag
    34981 aaacaaagta gttcaagtaa gtcaactcaa aagaataatc aaactagtaa taagaattca
    35041 aaaacaacaa atgctaaatc atccaatgca tcaaaaacgc caaatgctaa agttgagaaa
    35101 gctaaaagta aaatagagaa acgtacattc aatgactaag attactaaaa tcgttgaata
    35161 atcaatcata gcagtaagct atgtaatata tttttaaaaa ttgaatttca aaataaaagg
    35221 taggcattca ggtgcttacc ttttttgata tcatcagttt attttgaaag gcaatgcgat
    35281 catttcatgt atttatgttg tttgaaacat cgttagataa caatagtgat attgcacatt
    35341 ttaagctgaa gatggaaaaa tattcgataa tcaaataaac aatgaatttt agaaggtaca
    35401 atgacgttca ctaatttaaa tatagctgaa tgtgttggtg agtgatgttc actatagatt
    35461 tatattaata tacaaagaca aaggttgtta atttttatta agcgttaggt tgaatgtatg
    35521 agaattttag atttataata gaagatagaa acgaaaattt ttcttaaaag cagtaatgtt
    35581 gactcaaaat aagctataat aatgacactt atttaattga ttaacatttg ctaataaata
    35641 tcaatataga atataacttt ccaataatga ctgagaaaat cgaaatgtca gtctcgaatc
    35701 atataattag aaaattgatt attttctgtc aatttagggt tgaactatac atatgatatt
    35761 gttagaatat tttttaacat tatattttat tgctttaaag tggaatatac ttgaaataat
    35821 tagtagaggt gagtaaggat gagtaataaa ttagaatcat acagaagtga gattgtatca
    35881 ctgaatcatc aaattttaga cttattatct aaacgtggtg aactagcaca aaaaattggg
    35941 gaagaaaaat taaaacaagg tacacgtatt tatgatccac aacgtgaaaa agaaatgctt
    36001 aacgacttaa tcgatagtaa caaaggacca ttcaacgata atactattaa gcaattattt
    36061 aaagaaattt tcaaagcctc tacagattta caaaaatctg aaaatgaaaa acatttatat
    36121 gtatcacgta agttgaaacc tgaagatacg attgtaacat ttgataatgg gggcattatt
    36181 ggagacggca ataaatcatt tgtatttggg ccatgttcag ttgaatcatt tgaacaagtt
    36241 gaagctgttg ctaaaaactt acatgctaaa ggtgaaaaat ttattcgtgg cggtgcattt
    36301 aaaccacgta catcaccata tgatttccaa ggcctaggtg ttgaaggact taaaatactt
    36361 aaacagatta aagataaata tgatttaaat gttgtcagcg aaatcgtaaa tccaaatgat
    36421 tttgaagtgg ctgatgagta tttagacgta ttccaaattg gtgcacgtaa tatgcaaaac
    36481 ttcgagttat taaaagaagc tggccgtacg aaaaagccta ttctattaaa acgtggttta
    36541 tctgctacaa tcgaagagtt tgtttatgca gctgaataca ttgcttcaca aggtaatcaa
    36601 aacattattt tatgtgaacg tggaatccga acttatgaaa aggcgacacg taacacttta
    36661 gatatttcag cagtaccaat tttaaaacaa ggtacacact taccagtcat ggtagatgtt
    36721 acgcatagta caggtcgtaa agatatcatg ttaccaactg cgaaagcagc attagcagtt
    36781 ggtgctgatg gagttatggc tgaggtgcat ccagatccat ctgttgcact tagtgatgcg
    36841 ggtcaacaaa tggatttaga tgaattccaa gcattttatg atgaattaaa gcctttagct
    36901 gatttatata acgctaaaaa gttaaaataa tattccaagg aaactataga ctacttaact
    36961 aatatgtcat gttgaagtag aatattatct ttgaatcgac aattttaaac ttacagccat
    37021 tctaagagta tattactttt agagtggcta ttattttttg tatagaaata aaggtatact
    37081 gcacttaacg attgttataa tacttcgaca cttgttcaat ttcacaatta ttaaagatta
    37141 tgactgatag cagtaattaa aattataact atgaattatc tgtaaaatat aatagattca
    37201 cacatttgtt gctgaaatgt gaacattttt caacaaatgc aattgatatt tgaaaaggct
    37261 ttctcaaaac attacaatta aaaatgaaaa aagtttatat aaaattaaaa tatatcgttc
    37321 gttatcattt agcgtttgtt tttatttcaa gcttttcgct aaatttttcc aaacaaaaat
    37381 atgttactgt aaattaaaat atggtaaact atgaaaatga aatgaaaaca tgttattata
    37441 atgaataaaa cgtttacaag gaggaaatta tgacagttac tatatatgat gtagcaagag
    37501 aagcgcgtgt ctctatggcc acagtgtcgc gtgttgttaa tgggaaccaa aatgttaaag
    37561 cagaaactaa aaataaagtt aacgaagtca ttaagcgttt gaattatcgt ccaaatgctg
    37621 ttgctagagg tttagctagt aaaaagacaa caacagtagg tgtgatcatt ccagatatat
    37681 ctaatatcta ttattcacaa cttgctcgtg gacttgaaga tattgcaaca atgtataaat
    37741 atcactcaat tatttcaaat tcagataacg atcctgaaaa ggaaaaagaa atttttaata
    37801 acttattaag taaacaggtt gatggtatta ttttccttgg tggtacaatt actgaagaaa
    37861 tgaaagaatt gataaatcaa tcatctgtac ctgtagtagt atcaggaaca aatggtaagg
    37921 atgcacatat agcatcagtt aatattgatt ttactgaagc tgcgaaagaa attacgggag
    37981 aattaattga aaaaggcgct aaatcatttg ctttagtagg tggagaacat tctaaaaaag
    38041 ctcaagaaga tgttttagaa ggtttaactg aagtgttaaa taaaaatggc cttcaattag
    38101 gtgatacatt gaattgttct ggtgctgaaa gttataaaga aggcgtaaaa gcttttgcca
    38161 aaatgaaagg caatttgcca gatgccattt tatgtatcag cgacgaagaa gcaattggta
    38221 ttatgcatag tgcaatggat gctggtatta aagttccaga ggaattacaa attattagtt
    38281 tcaataatac acgattagtt gagatggtta gaccacaact ttctagtgtt attcaaccat
    38341 tatatgatat cggtgcagta gggatgcgct tattaacaaa atatatgaac gatgaaaaga
    38401 tagaagaacc aaatgtagtt ttacctcaca gaattgaata ccgaggaact acaaaataaa
    38461 ttcacaaaat taggcattca tctaacgacc caaattatat gggtgttgga agaatgcctt
    38521 ttatttatct tttaaaatcg ttgcagatta ggttacttat tgacgagtag attcgtacca
    38581 actcgctata tgtaaagcta attttttatt tttttcacta atttcttttg tgcgggggac
    38641 ataggtataa tcatttaaac gatcttccca tcttttaggt aataattcag atgaataatg
    38701 tttccattta ttaatccatt ctaacggtaa ataaccactt tgaattggtt gatcaattaa
    38761 acttaagaat acatgactcc atgcacgtgg tacgactctc caaatattgt agcctccgcc
    38821 accaaacata attacctttc cattcgtata agaatcagct aaatatttta caaaatatgg
    38881 aatttcatat aatgaatgta acgtacaatt tagatgagtt agtggatcac gataatgtat
    38941 atcgacacca tttacgctta gaataatatc aggtttaaaa ctctttacga caggctcaac
    39001 tgttaattta aaacactcca aaaatgatgc atcttctgta tacggttcaa gtgggacatt
    39061 tacagtgtgt ccatagccga tatcttcacc gcgctcagta tagtgaccag agcctgggaa
    39121 aagaaatttt ccggtttcat ggatagaata agtagtaaca tggttatcgg catagaaact
    39181 ccattgtgta ccatctccat gatgtgcatc ggtatctatg attaaaacgc gttgattgta
    39241 ttctttagct aagtattgtg cggtaattgc aatatcattg tatatacaaa aaccacttgc
    39301 tcgaccaggt tgagcgtgat gcaaaccacc acctaagtga caaccattta atactttgcc
    39361 tgacataata agatctgcta aagttaaagc gcctccaaca attgtggcac tatggcggtg
    39421 catatgctta aattgaccat tctcttcatc atttaatcca tatttcttag cctcatcttc
    39481 actgataatg ccatgtgaag catgcttaat agcttcgacg taatcatatt tatgaattaa
    39541 cattaattcg tcatctgttg caattctagg ttgtactatt tgttctggag acaataaatt
    39601 tgcattcaaa agtagctctg ttgttaattt taaacgcatt tgattgaagg gatgttggtc
    39661 atgaaatcga tattgtaata acttatctga ataaacatat gcagtttttg atgaatgttg
    39721 ttgcatataa tccctccgat attccaaaaa ttaaaagaaa aaccgattca tataacgaat
    39781 atcatcaaac gcttgttgct gttctaatgt aatgtttttg ccaattcttg ccattaaaca
    39841 attagctgga tgacttgtta tttctggatc atctgtagcg aatatttcaa gtccaccagt
    39901 tgccattaac cgctgcatta attttttata gtcaaataca tctaactttg aattttttaa
    39961 atcccaatgc cagtaatatt ctgtagttat aacgatataa ttctcgaatt ctggtgtaga
    40021 aaggctaagt tgtatcagct tttctgcaag ttgtaattgc ctaaaattga tgctgacttc
    40081 aattgccccc aattcgatta aatatggaag cttacctgta gaccagcgct caatttcatc
    40141 aggatagtgg aaagtgacat aacccacaat gagttgatct tggcgagcaa cataaattct
    40201 accttctggt aatgttgtaa tttctaacaa agctttatac tgatcttcag catctctaaa
    40261 tgcggttaaa tgcgcatcga aagtaagcgc tttcaaatct tcgtgtgtta aaggaccttc
    40321 aataacaaat tgcttgtcat gaatgtaata atcttcggat tgatacgtct ttaaatgatt
    40381 catattttca actcctcaat cgacgtcgtg attgtattaa attcattata tagaaaattt
    40441 acaataatta atactagaaa aaggataaaa gtaaaaattt tgaataatta gaaatgttat
    40501 gtataatatt gagaaagaaa gcgttttcac ataacaaagg gggagtttca aatgaaagtc
    40561 gaagtttata aaggagcgca aggtaaacat aaccttaaag attatgaaga aacatataat
    40621 acttttgatt ggaaagacgt agaacaagca ttttcttgga gtgaaactgg aaaaatgaac
    40681 atggcatatg aatgcataga tcgccatgta gatcaaggat taggggataa aatagcgtta
    40741 aattacaaag atgagcacag aaaagaatcg tatacttata aagatatgca acggttatct
    40801 aataaagcag cgaatgtttt gtctgaacat gcagaagttg acaaaggtga cagagtattt
    40861 atatttatgt cgcgtacacc tgaactatat tttgcgttgt taggtgtttt aaaaattggt
    40921 gcaattgttg ggccgttatt tgaagcattt atggaaaagg cagttgcgga tagattagag
    40981 aacagtgaag ctaaagtgtt aattactaat aaggcattgt tacctcgagt acctgtagat
    41041 aaattaccaa acttgaaaaa aattgttgtc gtagatgagg atgtagaaga caattacata
    41101 gacttcatta gtttgatgga aactgctagc gatgaatttg acattgaatg gttaaagtcg
    41161 gatgatggtt tgattttaca ttatacatca ggttctactg ggcaacctaa aggtgtattg
    41221 catgttcaac aagcaatgtt agtgcactat atttctggaa aatatgtatt agatttacaa
    41281 gaagatgatg tttattggtg tacagcagat ccaggttggg ttacaggaac atcttatggt
    41341 atttttgcac catggttaaa tggcgctaca aattgtatag ctggtggtcg cttttcgcca
    41401 gaacagtggt atagtatgat tgaagatttt aaagtgacga tttggtatac ggcaccaaca
    41461 gctttaagaa tgttaatgag tgctggtgac gatattgttg agaaatatga cttgtcatcg
    41521 ttacgttcga ttctatcagt aggtgagcct ttaaatcctg aagttataaa atgggcgaaa
    41581 aaagtatacg gtttaacggt gttagatact tggtggatga cagaaacagg tggacatatg
    41641 attgttaact atccaacgat ggacgtcaag cttggctcaa tgggcaaacc attacctggt
    41701 attcaagctg caattatcga tgatgcaggg aatgaattac caccaaatcg aatgggcaac
    41761 cttgctataa aaaaaggctg gccatcaatg atgtatcgta tctggaagaa tccagaaaaa
    41821 tataaatcat attttattgg agactggtat gtatctggtg attcggcata taaagatgaa
    41881 gatggttact tctggttcca aggacgtgtt gatgatgtaa ttatgacagc tggtgaacga
    41941 gttggaccat ttgaggttga gtctaaattg gttgaacacg aagcagttgc cgaagcagga
    42001 attattggta aacctgatcc ggttcgcggt gaaataatta aggcgtttgt tgcactgaga
    42061 aaaggatatg aaccaacaga cgaattaaaa gaagaaattc gtatatttgt taaagaaggt
    42121 ttgtcggcac atgcagcacc acgtgaaatc gaatttaaag ataaattacc taaaacacgg
    42181 tcaggtaaaa ttatgagacg tgtattaaaa gcttgggaat taaatttaga tgctggggat
    42241 ttaagtacaa tggaataatg acatgaatgt tattgaagat ttttttcgaa gaataaaggg
    42301 tgacaacata tttcatgtca atgtttaaat aatcgtttac tttacgataa gcaatataaa
    42361 gaactgttaa cttgtgtcat atcatttcgt agaaagcatt tgaaaatgat gacataacaa
    42421 taatggcata tctttatatt gctttttatt tttaatatga tctttggaag atgattattt
    42481 taaataatag aaaaatatag ttatcaatag tatcaagcgc taaaagttgt ataatacaaa
    42541 actttaataa gtgaatttat tgcaaaaatg aaagcgctaa cccgatttag tcgacaagtt
    42601 tttaacagtt cgttattata tgaatgtaag taaaaatttc ttagctacaa cttacatatt
    42661 ataaatgcat aaattaaaca aaaaggggcg aaaaaagttg actcatttat cagatttaga
    42721 tattgcgaat caatcaacac tacaaccaat taaggatatt gctgcatcag taggtatttc
    42781 agaggatgca ttagaacctt atggtcatta caaagctaaa atcgacatta ataaaattac
    42841 gccaagagaa aacaaaggga aagttgtttt agtaactgcg atgagcccaa caccagctgg
    42901 tgaaggtaaa tcaacggtta cagttggttt agctgatgca ttccatgagt taaataaaaa
    42961 cgttatggtt gcattaagag agcctgcttt aggaccaaca tttggtatca aaggtggtgc
    43021 gactggtggt ggttatgcgc aagtcttacc tatggaagat atcaacttac atttcaacgg
    43081 agatttccat gcgattacaa ctgcaaataa tgcattgtct gcgtttatcg ataatcatat
    43141 tcaccaaggt aacgaattag gaatcgatca aagacgtatt gagtggaaac gtgtattaga
    43201 tatgaatgat cgtgcactta gacatgtaaa cgttgggtta ggtggaccta caaatggtgt
    43261 accacgtgaa gatggcttta atattacagt agcgtctgaa attatggcga ttttatgttt
    43321 aagtagaagt attaaagact taaaagataa aattagtcgt attactattg gttacactag
    43381 agatcgcaag ccagttacag ttgcagattt aaaagtggaa ggtgcacttg caatgatttt
    43441 aaaagatgca ataaaaccaa acttagtaca atcaattgaa gggacacctg cattagttca
    43501 tggtggacca tttgcgaata tcgcacacgg ttgtaactca attttagcaa ctgaaacagc
    43561 acgtgattta gctgatatcg ttgtaacgga agctggattt ggttcagact taggcgctga
    43621 aaaattcatg gacattaaag tgcgtgaagc aggatttgat ccggcagctg tcgttgttgt
    43681 tgcgacaatt cgtgcgttaa aaatgcatgg tggtgtagcg aaagataatt taaaagaaga
    43741 aaatgtagaa gcagtaaaag caggaattgt taatttagag cgtcatgtta ataatattaa
    43801 aaaattcggt gtagaaccgg ttgttgcaat taatgcattt atacatgata ccgatgcaga
    43861 agtagaatat gtaaaatctt gggctaaaga aaataacgta cgaattgcct taactgaagt
    43921 ttggaaaaaa ggtggtaaag gtggcgttga cttagcaaat gaagtattag aagtcattga
    43981 tcaacctaat tcatttaaac ctttatatga attagaatta ccattagagc aaaagattga
    44041 aaagattgtg actgaaatct atggcggttc aaaagtaacg tttagcagta aagcgcaaaa
    44101 acaattaaaa caatttaaag aaaatggttg ggataattac ccagtatgta tggcgaaaac
    44161 acaatattca ttctcagatg atcaaacgtt gttaggtgca ccatcaggat ttgaaattac
    44221 aattcgtgaa ttagaagcga aaacaggtgc aggatttatc gtagcgttga caggtgcaat
    44281 catgactatg cctggtttac ctaaaaaacc agcagcatta aacatggatg ttactgatga
    44341 tggtcatgca attgggttat tctaataaat catgtcaatt gtttaataaa gataagtaaa
    44401 tagtttaata gaccggactg ttggagatgc attatttcag cagttcggtt ttttgctgtg
    44461 ctaaaaatag attcaatttg gcgaatctaa cgacaatgtt tgaaggtggt taattaatgt
    44521 atatgaagat aaaaagtggg cttgaagaat aggaaagcga tgcaatgaat attccatatt
    44581 aaaaaaaaat aataaaatag gttgcaatat ttaattggga tgcgctacaa ttaacactaa
    44641 taataggagt tgttaacaac atgaacaaac atcacccaaa attaaggtct ttctattcta
    44701 ttagaaaatc aactctaggc gttgcatcgg tcattgtcag tacactattt ttaattactt
    44761 ctcaacatca agcacaagca gcagaaaata caaatacttc agataaaatc tcggaaaatc
    44821 aaaataataa tgcaactaca actcagccac ctaaggatac aaatcaaaca caacctgcta
    44881 cgcaaccagc aaacactgcg aaaaactatc ctgcagcgga tgaatcactt aaagatgcaa
    44941 ttaaagatcc tgcattagaa aataaagaac atgatatagg tccaagagaa caagtcaatt
    45001 tccagttatt agataaaaac aatgaaacgc agtactatca ctttttcagc atcaaagatc
    45061 cagcagatgt gtattacact aaaaagaaag cagaagttga attagacatc aatactgctt
    45121 caacatggaa gaagtttgaa gtctatgaaa acaatcaaaa attgccagtg agacttgtat
    45181 catatagtcc tgtaccagaa gaccatgcct atattcgttt cccagtttca gatggcacac
    45241 aagaattgaa aattgtttct tcgactcaaa ttgatgatgg agaagaaaca aattatgatt
    45301 atactaaatt agtatttgct aaacctattt ataacgatcc ttcacttgta aaatcagata
    45361 caaatgatgc agtagtaacg aatgatcaat caagttcagt cgcaagtaat caaacaaaca
    45421 cgaatacatc taatcaaaat acatcaacga tcaacaatgc taataatcaa ccgcaggcaa
    45481 cgaccaatat gagtcaacct gcacaaccaa aatcgtcaac gaatgcagat caagcgtcaa
    45541 gccaaccagc tcatgaaaca aattctaatg gtaatactaa cgataaaacg aatgagtcaa
    45601 gtaatcagtc ggatgttaat caacagtatc caccagcaga tgaatcacta caagatgcaa
    45661 ttaaaaaccc ggctatcatc gataaagaac atacagctga taattggcga ccaattgatt
    45721 ttcaaatgaa aaatgataaa ggtgaaagac agttctatca ttatgctagt actgttgaac
    45781 cagcaactgt catttttaca aaaacaggac caataattga attaggttta aagacagctt
    45841 caacatggaa gaaatttgaa gtttatgaag gtgacaaaaa gttaccagtc gaattagtat
    45901 catatgattc tgataaagat tatgcctata ttcgtttccc agtatctaat ggtacgagag
    45961 aagttaaaat tgtgtcatct attgaatatg gtgagaacat ccatgaagac tatgattata
    46021 cgctaatggt ctttgcacag cctattacta ataacccaga cgactatgtg gatgaagaaa
    46081 catacaattt acaaaaatta ttagctccgt atcacaaagc taaaacgtta gaaagacaag
    46141 tttatgaatt agaaaaatta caagagaaat tgccagaaaa atataaggcg gaatataaaa
    46201 agaaattaga tcaaactaga gtagagttag ctgatcaagt taaatcagca gtgacggaat
    46261 ttgaaaatgt tacacctaca aatgatcaat taacagattt acaagaagcg cattttgttg
    46321 tttttgaaag tgaagaaaat agtgagtcag ttatggacgg ctttgttgaa catccattct
    46381 atacagcaac tttaaatggt caaaaatatg tagtgatgaa aacaaaggat gacagttact
    46441 ggaaagattt aattgtagaa ggtaaacgtg tcactactgt ttctaaagat cctaaaaata
    46501 attctagaac gctgattttc ccatatatac ctgacaaagc agtttacaat gcgattgtta
    46561 aagtcgttgt ggcaaacatt ggttatgaag gtcaatatca tgtcagaatt ataaatcagg
    46621 atatcaatac aaaagatgat gatacatcac aaaataacac gagtgaaccg ctaaatgtac
    46681 aaacaggaca agaaggtaag gttgctgata cagatgtagc tgaaaatagc agcactgcaa
    46741 caaatcctaa agatgcgtct gataaagcag atgtgataga accagagtct gacgtggtta
    46801 aagatgctga taataatatt gataaagatg tgcaacatga tgttgatcat ttatccgata
    46861 tgtcggataa taatcacttc gataaatatg atttaaaaga aatggatact caaattgcca
    46921 aagatactga tagaaatgtg gataaagatg ccgataatag cgttggtatg tcatctaatg
    46981 tcgatactga taaagactct aataaaaata aagacaaagt catacagctg aatcatattg
    47041 ccgataaaaa taatcatact ggaaaagcag caaagcttga cgtagtgaaa caaaattata
    47101 ataatacaga caaagttact gacaaaaaaa caactgaaca tctgccgagt gatattcata
    47161 aaactgtaga taaaacagtg aaaacaaaag aaaaagccgg cacaccatcg aaagaaaaca
    47221 aacttagtca atctaaaatg ctaccaaaaa ctggagaaac aacttcaagc caatcatggt
    47281 ggggcttata tgcgttatta ggtatgttag ctttattcat tcctaaattc agaaaagaat
    47341 ctaaataatt aactaaatat agcatatgta tgattaactt tgtagacaat gtgaaagcaa
    47401 ttaatttata aactattgat tggtttaatg gctttccttt agagtaaata aaaagaacag
    47461 cagtgagaaa ttttctaatt gaaaataatc ttactgctgt ttttaatatt tggattcatt
    47521 gttgtggtta ctttaaaaag tgagcatcaa ttaacgcttt tttcgattta acaaatgtga
    47581 tttaatatca tattttaatg cgtcgttgta ttctttttca gtgatttgat cttcgattaa
    47641 catacgcttt aatacataat gttgtctttg aatactatat ttcaaatctt tatccgattt
    47701 taacgttcca tctttttcgt agggtgtata gccatatggg ctttgcaaca aaccgataag
    47761 gtatgcagat tgtgcaattg ataaatcttt tggtggaata ccaaatagac tatatgaagc
    47821 ggatgcaatt ccggaaatat tagcgccatt ataatctcta ccgaagggaa ctatatttaa
    47881 atatgtatat ataatttcat cttttgagag taggtgttct aatctaattg ctaggcgaag
    47941 ttcatttgct tttctactat atgttttttc gttggtaaga acttgatttt taacaagttg
    48001 ttgtgtaatt gtgctaccac ctgaactttg atcagtatta aaaatatctt gtatcattgc
    48061 tcttaaaatc gcctttggta agatgccatc atgtttataa aataaagtgt cttcagatga
    48121 cgttaatgct ttaatgacat ttggacttga tgttttaggg cctataatga gtgagttttg
    48181 agaatggtta tactcatata ataaattttt gttattatga tctaataatt catcgccagg
    48241 tatttgtcga acttttttta ttaaagcatc atctgataat gagtcggacg ttttagttaa
    48301 atgatgaaaa tataaagaca tcgcaatcac agcgatagca acgatggcaa tgaaaatcat
    48361 aaatataaaa acaatgacat gtttgatagc tttatatatc ttttcaaaat gatatatacg
    48421 atgattcaat tgatgattgt tgtcttgatt cgtcatcttt aacctcctaa attacgtata
    48481 gtataccata tttaagcaat ttgactttta ttttaacgtt aattataatt agggaaaatg
    48541 cataaagaat aggataaaag gacaagtaat attatttgaa atcatcagag agctagtggt
    48601 cgctgtgaac tagtgttttt aataatatga atacaccttt ataactaaca tgtcctacgt
    48661 ttattaatga acgtatcatt aatagagtga acattatctt atgattatgt ttaagtttgg
    48721 tggtaacgtg caatcgcgcc cttacatatt tgttgtaagg gtttttttta cgttcaaaaa
    48781 actttaaagt attttaacaa gtattcaata aataattata aataggagga aataataatg
    48841 acgaatgtat taattgaaga tttaaaatgg agaggtctta tttatcaaca aactgatgaa
    48901 caaggtattg aagatttatt aaataaagaa caagtgacgt tatactgcgg tgccgatcca
    48961 acggcagata gtttacatat tggtcactta ctaccattct taacattaag acgttttcaa
    49021 gaacatggac atcgtcctat cgttttaatt ggcggtggta caggtatgat tggtgatcca
    49081 tcaggtaaat cagaagaacg tgtgctacaa acagaagaac aagtagataa aaatatcgaa
    49141 ggtattagta agcaaatgca caatattttt gaatttggaa cagaccatgg tgcagtgctt
    49201 gttaataata gagactggtt aggacaaatc tcattaatta gttttttacg tgactatggt
    49261 aaacacgtcg gcgttaatta catgttaggt aaagattcaa tccaaagtcg tttagaacat
    49321 ggtatttcat atacagaatt cacatacacg attttacaag ctattgattt cggtcatttg
    49381 aatagagaat tgaattgtaa gattcaagta ggtggatcag atcaatgggg taatatcaca
    49441 agtggtattg aattaatgcg tcgtatgtat ggtcaaacag acgcatacgg tttaactatt
    49501 ccgcttgtaa ctaaatcaga tggtaagaaa tttggtaagt ctgagtcagg tgctgtttgg
    49561 ttagatgctg aaaaaacaag tccttatgaa ttttatcaat tctggattaa tcaatcagac
    49621 gaagatgtaa ttaaattctt aaaatacttt actttcttag gaaaagaaga aattgatcgc
    49681 ttagaacaat ctaaaaatga agcaccgcat ttacgtgaag ctcaaaaaac attagctgaa
    49741 gaagtaacta aatttattca tggtgaagat gcattaaatg atgcaatccg tatttcacaa
    49801 gcattattta gtggtgattt aaaatcatta tcagcgaaag aattaaaaga tggatttaaa
    49861 gatgtgcctc aagtgacatt atcaaatgac acaacaaata tcgttgaagt ccttattgaa
    49921 acaggcattt ctccttctaa acgacaagca cgtgaagatg ttaacaatgg tgcgatttat
    49981 attaatggtg agagacaaca agatgttaat tatgctttag caccagaaga taaaattgat
    50041 ggcgaattta cgattattcg tcgcggtaag aaaaaatact tcatggttaa ctatcaataa
    50101 atataattgc atagctaaat aaattagagc ctactcatat tcattcctaa gaatgtaatg
    50161 agtaggctct taatgtactt ttctgtctgt aaattatcta aagaaatctc tatcgccttg
    50221 gccaggtgat tgacgttctg atcggctttg acgttttggt tgttcttttt gttgttttaa
    50281 tttcacttta acttcttttg ttttaccatc acggataacg gtaacagtga ctgattcacc
    50341 aggtttttta ttttcatata aatagcttct taaatcaaca tcatctttaa ttttcttgcc
    50401 atcaatttct gtaataatat cacctttttt aagatcaata tcactatcag ctttggcgac
    50461 ataaataccg tcttctctat cagtatgaag ttgctcgcgc tcttcttcag gaatatcttt
    50521 caaattaatt aaaccaatac caatcgaagg gcggtcaatt ttaccatgtt ttacaagttg
    50581 ttcaattgtt actttaactt cattacttgg aatagcaaac ccgatacctt caacttgtgt
    50641 cgcagcaatt ttcattgagt taataccaac taaattacca ttaatatcta ctaatgcgcc
    50701 acctgagtta cctgggttaa tagcagcatc tgtttgaaga acgctaactt ttgtattgcc
    50761 accagttgtc tcagcgtcaa tcgtacgttc gcttgctgaa atgataccag atgttacaga
    50821 gttagcaaat tgtaatccta atgggttacc cattgcgaat acgctatcgc cagtttgtac
    50881 ttttgaagag ttggcaaatt gaatcgcttt aatacctttt gtattttcaa ttttaagtac
    50941 agcaatatca gttactgcat ctttaccaac taatttcgct ttaacttgtt ttttattatg
    51001 taattggact ctaatttcat ttgcgccatc aataacatga ttgtttgtaa cgatataagc
    51061 tgaattgttg tttatttgat agataacacc tgaacctact ccagcttcag atggtttaga
    51121 tgatttgcct tttaataagt cgtctacact tgatgctttt tgcatgttaa taactccaac
    51181 aattgtaggg gagacagatt ttatcatttc atgaacggta ccgaatttct tgctttgacc
    51241 gtctaattga ttgccacctt tattatttgt tgtctgaaca gttgaaccat ctttatttaa
    51301 aattgtacta tttaatactt tgcctatacc aagtactaga agtgcaccaa taattccagc
    51361 aatcaatgca acgatgactg ttttaaacca tggaaattta ggtcttctgt atctaggtgt
    51421 ttggctatgg tttgttgtag aatgatctgt atgattaaaa tctgacatac ttaacctcca
    51481 ttatatgatt tatatatgct ttaattatgt ctttttttta taattattgc tagtaataca
    51541 ctatacaact ttagatgtat tttcagacta tcttgttcta gtaaaaatat agtgataaaa
    51601 tctagttgtt ataaacaaat aagttaagta ggtggcacaa tgtattcagt gattagtaag
    51661 attttgaatt tcattttagt taaaatgtca aaatcattat acgtaatcgg taaagataat
    51721 attcctaagg atagtaaata tgtcgtaact tgtacgcatg aaagttataa cgaagtcatt
    51781 atgctaggta tggcattata tcctaatcaa attcattata tggctaaaaa agaattattc
    51841 aaaaataaat ggattggtaa atttttaacg tctttaaatg catttcctgt agatcgtgaa
    51901 aatccaggac ctagtacttt aaaacgccca atcaatttgt tgaaagataa taagacagtt
    51961 ggtatcttcc caacaggttc tcgcacatcg caagaaggtg cacctttgaa acgtggtgct
    52021 tcaacaattg caatgttaag taagtcacca atattacctg ttgcttatgt tggtcctact
    52081 aagattcacg gtcttttaac aggacaagca tacataaata ttggcaaacc aatcgatatt
    52141 aacgatttac ctaaagattt aaaacgaaac gagcgtatcg attatattac aaaagagatt
    52201 gaaacacgaa cagcaaagtt acaacaagaa ttgcatgaaa ttgtaaaaag tttataaaat
    52261 aacttgaggt agaaatatag ctggttagta agcctaggtg actataacgg agtatcgaag
    52321 tagttattct agttacatta gtaatcttta tatttctaca ttattttaag aaggatgaca
    52381 ttcaatgtat aaatttttcc aaaatttagg gcgttcgtta atgttaccag ttgccatttt
    52441 accggctgca gcaattattg ccggaattgg taatacgtta aatgcacttc atgcgacgcc
    52501 gaaaatcgca atgttcttta ctacggtagg tactacgatt ttagagcaat taggtatttt
    52561 atttgcaatt ggtgtagcta taggaatggc aaagaaaaat gatggtgtag tcgcgcttgc
    52621 agctacgtta ggatattttt tagttacagt tgtattatca ccaatgaaat tagcgccttt
    52681 attaggaatg aaagcttcgg aaataaattc agcttttgaa aagatgaata atggcaatgt
    52741 ctttgttggt attgtaatag gtttaatagc agcttatgca tacaataagt ttagtgaaac
    52801 agaattacca ttagcattat cattttttag tggtaaacgt ctagttccaa ttatgactgc
    52861 attttactgt acatttttag ttgtcatatt gttattctta tggccactac tttattcatg
    52921 gattgtaaaa tttggtgaat ctatagtcgg tttaggatcg tttggagctt tcatatatgg
    52981 tgttgccaat agattattaa ttcctacagg attacatcat gcactgaata gtgtgttctg
    53041 gtttgatacg attggtatta atgatattgg taaattccaa agtggcaaag atgctataaa
    53101 aggtattaca ggacgctatc aagcgggatt cttcccaatc atgatgttcg gtattccagc
    53161 ggctgcgtta gcaatgtatc atacagctaa aactactcag aaaaaacaag tttacggttg
    53221 gttcctagcg agttcagttg cagcattttt cgttggtgtt acagagccga ttgagtttgc
    53281 atttatgttt gttgcgccaa tcttatatgt tgttcatgcg ttattaacag gattatcttt
    53341 atttatcgca gctacattcc attggacagc tggattttca tttagtgcag gtttgataga
    53401 ttatgtgtta tcactaatta atccagtatc aaatcatcca ttgatgttgc ttgttcaagg
    53461 tgttgtattt ttcatcttat actatgtgat tttccgtgta gtaatccaag tatttaactt
    53521 gaatacgatt ggtagaggtg aaaatgaatt agttgaccca acagttgtaa aagataatat
    53581 tgctcctggt gaaaatgata ttaaacaaag taaatatcat caacatgcta tacaaatatt
    53641 agaaggttta ggcggtcaag agaatattgt taatttaacc aattgtgcaa caaggttgcg
    53701 tctagagtta aaagacacat ctattattga taaacagaag attaaaaacg ccggtgcagt
    53761 tggtgttact gttaatggca aacattcaac tcaagttatt gtcggtacac acgtacagca
    53821 ggttgctgat gaaattgaaa aacatttata aaatttaaac agctaaagcg caaatctaac
    53881 ttcaataggg tttgcgcttt ttttagtcaa atagtagaat gatttatatc tgaattttca
    53941 gaaatggagt ggggattgtg aagaaaattt tatttgatgt tgatggcgtt tttttaagtg
    54001 aagaacgttg tttcgatgtc tctgcattaa cggtatatga attattaatg gataagtgtt
    54061 atttaggtct tcattcacat atagattggg aaactttgac tgataacgat atacaagaca
    54121 ttaggaatcg aatttttcaa aaagataaga ttttaaacaa attaaaatcg ctagggctga
    54181 attcaaattg ggatatgtta tttatcgttt ttagtattca tttaattgat attttgaaaa
    54241 agttatcaca tgatgaaatt gaggcattca tgtatcaaga tgagcctgtc gaattgaagt
    54301 tgcaaaatat aagcacaaac ttagcagact gttttaattt aaatgaacaa ctacctttac
    54361 aatttttgga taatgtaaaa gttggtaaaa ataatattta tgctgctttg gaagagtttg
    54421 caacaacaga gttacatgtt tcggatgcta ctttatttag tttgaagggt gcattatgga
    54481 cgttagcgca agaagtttat caagaatggt atttaggatc gaagttgtat gaagatgttg
    54541 aaaagaaaat agcacgaact acttttaaga caggttatat ttatcaagaa attattttga
    54601 gaccagtaga tgaagttaag gtacttctga atgatttaaa aggtgctggt ttcgaattag
    54661 gtattgcaac aggtcgtcct tatactgaga ctgttgtgcc atttgaaaat ttaggattgt
    54721 taccatattt tgaagctgat tttattgcaa cagcaagtga tgttttagaa gcagagaata
    54781 tgtatccgca agcacgacca ttaggaaagc cgaatccttt tagttatatc gcagctttat
    54841 atggtaataa tcgcgataaa tatgaatctt atatcaataa gcaagataac attgtaaata
    54901 aagatgacgt atttatagta ggcgattcgt tagctgactt attaagtgct caaaaaatag
    54961 gtgcaacgtt tattggaaca ttaacaggtt taaaaggtaa ggatgctgca ggtgagttag
    55021 aagcgcatca tgccgactat gttattaatc atttaggtga acttagaggt gtactagata
    55081 atttgtaatt tgattgttgt ttgacagcat aacttgtagt gaatgattga accaaaggtt
    55141 tcatattgag ttacaatgaa attaataatg aaaaaatgcc aagaagcaat ggaagtaatc
    55201 caatgtcttc ttggcatttt gaatttacat aaattgttta tgactgtacc gtcaattcag
    55261 ttgtgaaaat ttgattgtat tcaccaactt gtttaagttc atcaattata ttgtttgaaa
    55321 caggttgatc aacggataaa atcattagcg catctccgcc cgcttcagtt ctacctaaag
    55381 tcatagatgc aatgttgata ttgtatttac ctaacaatgc gccagttttt cctaccatac
    55441 ctggagtatc attatgatat gacacaattt gatattgatt tggctttaag tctacagaaa
    55501 aattattaat tctaacaatt cttggaccga aacctgtaaa gacagaagcg ccaactttaa
    55561 cggaatcgct atcgtttgat agttctacct ctaagtagtt actaaaacct gtctctgctt
    55621 tattattttc aatatttaat gtcacttgtt gttgatttaa caacattaga gcattgataa
    55681 tattgacttc aggaccaaga tcatctttta aaatatgagt aataattgta cgtgttaata
    55741 aactactatc aatagaggct aagtcgccac cgtacgtaat tttaatagag cttggtgcat
    55801 tgtacattaa ttgaatagct aattcaccaa ctgtttggct taaattgatg aatgatttta
    55861 cagtatcatc tatattgctt aagtccattt taggtgcatt cactgcatgc gttacagtac
    55921 catcaattaa aatttcgatg atttcatttg aaacagaaat tgccactttt tcttgagctt
    55981 cgactgttga agcacccaaa tgaggtgtaa caataatttt atcatgtgca acaagaggcg
    56041 agtcagttgc aggttcatgt tcaaacacat cgatagctgc ccgactaatt tgtccttcgt
    56101 ctaatgcttt tattagcgcc ttttcatcaa taataccacc acgtgccaca ttgattattt
    56161 gcaaactagg ttttgctttg gcaaaaaaga cagcattaat taagcctttt gttttaggtg
    56221 ttagtggtgt atgtaatgta acgaaatcag aatgttgggc aatctcatca actgttgcct
    56281 tcgtaatgct taaagatttt gctttttcat ccgttaagta agggtcaaaa gctagtattt
    56341 tcattccgaa actttgcgca cgtttagcaa cacctaaacc aattctacca gcaccaatga
    56401 cacctaatgt tttatgataa agctcagtac ctttaaatgc atttcgattc cattctttat
    56461 ttgtaagtga ttggtgtgct tgcggaatat ttcgtgccat tgataataac attgccagtg
    56521 tatgttcagt agctgaaatc gtattaccat ctggggcatt aataactaaa atacctttta
    56581 atgttgcagc atcaatatta atattatcta caccaacacc ggcgcgtgcg attactttta
    56641 aagaatcagc agcatttatg atattttcag taaccgtagt ttgactacga acgattaaag
    56701 catggtatga aggtataatt ttgattaatg cttcttcgga caagccagtt tgaatatcta
    56761 cattgaattg ttcgtgatct aataatgctt tgataccatc ttttgatatg ggatctgcaa
    56821 cgagtacatt aaattgcttc atgaataacc tccatatatt ttgatatacc tttaccgata
    56881 tagttaactt tacggtgttc agttaaaata atttctaaag cagatactac cgataaaata
    56941 tcgaaaggac taattttccc catatgacca attcttaaaa tttgaccttt aagatggcct
    57001 tgaccaccag caattgttat tttaaagcga tttttaagtt catctttgat tattttcact
    57061 tcatcatttg tattaggttt gaatgctgta accgttggag atgcatcttt atcattgact
    57121 aataaagtta aatctaatgc ttttaaggcg cttcttaatg catttctaat tgcatagtgt
    57181 cgtgctatta cgtgattgaa accttctgct tttacggttt caacgtatgc atttacacct
    57241 ctaaataaag acacatttgg tgtgaacggt gtagaattgt cagcttgtga cgaaatgtat
    57301 ttatttaagt ctagataaaa ttttggcgta gttacttctt tgaaatgttc ttttgcacgg
    57361 tggctataag ctacaaaagc taatcctgga ggtaacataa ttgctttttg actaccagaa
    57421 acaagtacat caattttatc tttgttaatg tcaacatcaa cagcaccaat gcaacttacg
    57481 ccgtcaacta caaaataaat attactatta aattgattaa tggcatttcc taactcgtga
    57541 atagggtgta gcactgtcgt agatgtttcg caatattgac taaatactgc tttaacttca
    57601 acatttaaag ttgaaaggaa attgatgaaa tctttgacat ctacagcttc tccccatgtt
    57661 acgtcataaa tatgcacatt tttgtaataa gtttgtgcaa tttgtttaaa tcggttacca
    57721 aaggcacctg aaacaatgac aacgaagtga tcttcagggt ttacaatgtt caacatacta
    57781 gcctccaaga cgcttgtacc gctagatgtt aaaataagta catcattttg actcccaaat
    57841 attggcttta gaccttgaaa tgcttgttgt gcgatgtctt caaaatcttt agaacgatga
    57901 ccaaccatag gtgcttgaat ttctctcata atggcatcag gtacaggggt tgggccaggt
    57961 gttaataaca acggttgatg ataatacatt tatatgcctc ctataaataa gatttcacta
    58021 ttttagcaaa ttttctgaaa atttaaaagc ctaaaaaagt gataacgcta tattgtcgaa
    58081 caaacgttat cacttaacga ttttatttgg cctgaatgat gggatagatt tttatgtcca
    58141 cattatttct tacagcattt gaaatcatgc aattattatc tgcaattgtt atcaattttg
    58201 gtaatcgctt ttctaattgt gctatttgat cacttggaat ttgaatttga ggatggtgca
    58261 caatttttga catactgaat tttccgttat ttaaacaagc tgttccaatc gattgttgtt
    58321 caattgaaat atctgtgaac tttgcacgtt caagagtagc tgctaatgag atgatataac
    58381 atgatgaagc ggctgatact aacatttcat cgggatttgt tcctatacca acaccaccta
    58441 aagaagcagg tatagaaata ttctctgaaa gtatgtcgcc ttgaacgttt ccgacattgt
    58501 tacgaccacc ttgccaagaa gtttggactt taaagtcatg ttgatgcaat tgctttaacc
    58561 tccaatataa ttgtgatagt ttaattttag aatagtttat caaagtttaa cagaaaggtg
    58621 acttatcaat gactctgaat aaactgaaag atgaattaca aattgtttcg caccgtggat
    58681 tgccgagtga ttttcctgaa aatacaatgg tcggttatcg agaggtaatg gggctcaatg
    58741 ttgctatgtt agaaatagat gttcatttga ccaaagacca acattttgtt gtgatacatg
    58801 atgaaacaat tgatagaaca tcggatggta aggggcgtat tgctgattac acattatcgc
    58861 aattaaaatc atttgatttt ggtagttata aagatgttgc ttttaaggga gaacgaatac
    58921 ccacgttaga tgaagtactt tcattatgct taaagtatga taaaaagcta ttgattgaat
    58981 tgaaaagtcc gaatttatat ccagaaatag aatgtaaatt gctggcattt ttggaagaaa
    59041 agaaagtcga tgcgacacaa gtagttatac aatcttttga tatcgaatgc atcgaaaagt
    59101 taaacacatt gggcagtata tatgaattag gtgtgctttg tagtaaacgt aattattggt
    59161 ataaaaagcc aaacttttca agaattgctc aaattgctag ttatgttaat cctaattatg
    59221 cattagtgac cagaaaattt gttgataaag cacatcatca tcaattacag gttatgccat
    59281 acacagttaa taaattgaaa actggagaaa aattaagaca aatgggtgta gatggattaa
    59341 ttacagataa tccgaaatta tttattaagt gagacgatga atggtaattt gatagaggga
    59401 tgaggattcg tatatcgcaa ttgataattt taaaaactaa tgatgaaata ctaaagataa
    59461 ttaaacaaat aagacttgta aattctaagt tgattgtaaa atagggtagc tccatataat
    59521 tttgaatcta tcatttttaa attaataaaa aaaacaccca caattgtggg tgtttgtatt
    59581 gaacgtatta tcttgagtag tactcaacga ttaattgttc gttaatttca gcaggtaatt
    59641 cgctacgttc tggtaaacgt acgaaagtac cagttaagct gtcagcatca aagtttaagt
    59701 actcaggtac gaaattgttg atttcaactg attcaacgat gatgtttaat ttttgagatt
    59761 tttcacgaac tgaaattgtt tgaccaggtt taacagaata agatggaata tcaacacgtt
    59821 taccatctac taagatatga ccgtggttaa ctaattgacg tgcttgacga cgagtacgag
    59881 ctaaacctaa tgaataaaca acagcgtcta aacgacttgc taataagatc atgaagtttt
    59941 caccgtgtac accgaatttt ttaccagcga tgtcaaatgt gttacggaat tgtctttcag
    60001 tcattccata taagtaacgt aatttttgtt tttcacgtaa ttgtaaacca tattctgata
    60061 attttttacg ttggtttgga ccatgttgtc ctggtgcgta aggacgtttt tctaattctt
    60121 taccagtacc gcttaaagag atacctaaac gacgagattt tttccagttt gaacctctga
    60181 atcgagccat aataagactc ctcctttaat tcttttttgt tgttatgaat aaacaaaaaa
    60241 gagtgttgca tgcttactaa gatatattgt tttatgtgtc ttcaccttat agcgacagtt
    60301 acgtgacacg tccgcattgg gaacaacata gcgctcaata atatacaact gctactttcg
    60361 ttaattcaca caaagattat tgtaacattt tcctgctaac tgtcaacact ttattgaaaa
    60421 tgctgatgtc cttttatgcg agttgctttt caataatttt aacaattgct tcaagatgtt
    60481 ctttgtcatt gtcatcaaat cgatccgtta taggggcatc gatatctaag acgccgataa
    60541 ttttatcatc tttgaaaatt ggaacgacaa tctcagactt actattagca tcacaagcga
    60601 tatgtccttc gaattgatga acatcagcta caatttgtgt acgacgttct gaaacggctg
    60661 taccacatac accttttcca attggaatgt ggacacaagc ggggtgtcct tggaaaggtc
    60721 caagtataag ttcattttgt tccaataagt aaaagccaac ccaattaatt tgatctagat
    60781 tatcatttaa taaggcagac atgttactta aaatagcaat catgtgatgt tcatcttcaa
    60841 taaggcttgc tgcttgtttc tttaataatg tgtagtttgt tgggttaatt gttgtcatat
    60901 ttaaaacctc ttattttcat agattttata atagtataga atttcgaaaa tattatggca
    60961 ataaattcat tttataattt atccttaagc aaaattacgt tataatagta atgataaatt
    61021 aggaggagaa gcatatggtg ttatatatca ttttggcaat aattgtgatt atattgattg
    61081 ctgtaggtgt attattctat ttacgttcaa ataaacgaca aattattgaa aaagcaatcg
    61141 aacgtaaaaa tgaaattgag acgttacctt ttgatcaaaa ccttgcacaa ttatctaagt
    61201 tgaatttaaa aggtgaaaca aaaacgaaat acgatgcaat gaaaaaggac aacgtagaaa
    61261 gtacaaataa gtatctagct cctgtggaag aaaaaatcca taatgctgag gctttattag
    61321 ataaatttag tttcaacgca tctcaaagtg aaattgatga tgcaaatgag ttgatggata
    61381 gttacgaaca aagctatcag caacaattag aagatgtaaa tgaaattatt gcgttataca
    61441 aagataatga tgaattatat gacaaatgta aggttgatta tcgtgaaatg aaacgtgatg
    61501 ttttagcaaa tcgtcatcaa tttggtgagg cagcaagtct acttgaaact gaaattgaaa
    61561 aatttgagcc aaggttagag caatatgaag tactaaaagc tgatggtaat tatgtacaag
    61621 cgcacaacca tatagctgcc ttgaatgaac aaatgaaaca gctaagatct tatatggaag
    61681 aaataccaga attaattaga gaaactcaaa aagaattacc tggtcaattc caagatttaa
    61741 aatatggttg ccgtgatctt aaagttgaag ggtatgatct ggatcacgtg aaagtagaca
    61801 gtacattaca aagcttaaaa acagagctta gtttcgttga accattaatt agccgcttag
    61861 aattagaaga agctaatgat aaactagcta atatcaatga taagttagat gacatgtatg
    61921 atttaattga acatgaagtt aaagctaaaa atgatgtcga agaaacaaaa gatatcatta
    61981 cggataactt attcaaagct aaagacatga attatacatt gcaaacagaa attgaatatg
    62041 tacgtgaaaa ctactatata aatgaatctg atgctcagag tgttcgtcaa tttgaaaatg
    62101 aaattcaaag tttaatttct gtatatgatg atattttaaa agaaatgtct aaatctgctg
    62161 tgcgatatag cgaggttcag gataatttac aatatttaga agatcatgtc acagttatta
    62221 atgacaaaca agaaaagcta caaaatcatc tgattcaatt gcgtgaagat gaagcagaag
    62281 cagaagacaa tctgctacga gtacaatcga agaaagaaga agtgtatcgt cgattacttg
    62341 cttctaactt aacaagcgtt cctgaaaggt ttatcatcat gaaaaatgaa attgatcatg
    62401 aagttcgtga tgttaacgaa caatttagtg aacgtccaat acacgttaaa cagttaaaag
    62461 ataaagtgtc taaaattgtg attcaaatga atacatttga agatgaagca aatgatgttc
    62521 ttgttaatgc tgtttatgca gagaaattaa ttcaatatgg aaatagatat cgtaaggact
    62581 atagcaatgt tgataagagc ttaaatgaag ctgaacgatt atttaaaaat aatcgctata
    62641 agcgtgcgat tgaaattgca gagcaagctc ttgaaagtgt tgagccaggt gttactaaac
    62701 atattgaaga agaagttatt aagcaataga aactagtatg tagttatact taaataatat
    62761 gagcactctg tcaaattgga ctgatgagtt taataattga agttagccaa cgatacgttg
    62821 tctagcttct tttttatatg gataaatgaa agggacaata aatataaata gcaattgttt
    62881 aaagataaac gtaatcaaat gtgttgtttt aattaatata agtagtgaaa aaagcataat
    62941 cacacagctg tttaaataga gtgaaatagt ctaattctta tttaataagt agaaataaga
    63001 ttattttgcg ctataacctt tattttttgt cggataatag tataatgctt gagtaaaaca
    63061 attttaacga ttgatgatta atgaaagaga agtgtttatt ttgatatatc tagataatgc
    63121 ggcaacgacg aaagcatttg aagaagtgtt agatacttat ttaaaagtaa atcaatcaat
    63181 gtattataat ccgaatagtc cgcataaagc tggtttgcag gcaaatcaat tactacaaca
    63241 agcaaaaacc caaattaatg caatgattaa ttcaaaaaca aattatgatg ttgtattcac
    63301 tagtggtgca actgaatcca ataatcttgc tttaaaaggt attgcctatc gtaaatttga
    63361 tacagcgaag gaaataatta catccgtgtt agagcatccg tccgtattag aggttgtaag
    63421 atatttggaa gcacacgaag gatttaaagt taaatatgtt gatgtaaaga aagatggcag
    63481 tattaactta gaacacttca aagaattaat gtcagacaaa gtcggtttag taacatgtat
    63541 gtatgtaaat aatgtaactg gacaaataca gcctattcca caaatggcta aagttataaa
    63601 aaattatcct aaggcacatt ttcatgtaga tgcggttcaa gcattcggca aaatttcaat
    63661 ggatctcaat aacatagata gtattagttt aagtggacac aagtttaatg gtttaaaagg
    63721 acaaggcgtc ttacttgtaa atcacattca aaatgttgaa ccaactgtcc atggtggtgg
    63781 tcaagaatat ggtgttagaa gtggaacagt taatttgcca aatgatattg caatggttaa
    63841 agcgatgaag atagctaatg aaaactttga agcattgaat gcatttgtta ctgagttaaa
    63901 taatgacgtc cgtcaatttt taaataaata tcatggagtt tatattaatt cttcaacttc
    63961 aggttcacca ttcgttttaa atattagttt tcctggcgta aaaggtgaag tattagttaa
    64021 tgctttttca aaatatgaca ttatgatatc tacgacaagt gcttgttcat ctaaacgtaa
    64081 taaattaaat gaagtattgg ctgcaatggg attatcagac aaatctattg aaggtagtat
    64141 aagattatca tttggggcta ctacaactaa agaagatata gcgaggttta aagaaatatt
    64201 tatcatcatt tatgaggaaa ttaaggagtt gctaaaataa tgaagtatga tcacttgctt
    64261 gttagatacg gggagttaac attaaagggt tcaaatagaa agaaatttgt aaatcaatta
    64321 agaaataatg taaataagtc attaaaagga cttgatgggt ttgtcgttaa aggcaaacga
    64381 gatcgtatgt atattgaact tgaagaccat gcagatataa atgaaataac atatcgatta
    64441 tcaaaaattt tcggtattaa atctattagt ccagtattaa aagtagaaaa aacaatagag
    64501 gcaataagtg cagcggcaat taaatttgcg cagcaatttg aagaaaacag cacatttaaa
    64561 attgatgtga agcgtgccga taaaaatttc ccaatggata cgtatgaatt acagcgtgaa
    64621 ttgggtggtg cagtattgaa gcacttcgac aatatttcag tgaatgtcaa acgtccagat
    64681 catgaaattc gagtggaagt tagattagat gcaatttata tgtatgaaga agttgttccg
    64741 ggttcaggtg gattaccagt tggtactggt ggtaagacgt tactaatgct ttcaggcggt
    64801 atagactcac cagttgctgg gatggaagtg atgagacgtg gcgtaacaat tgaagcgatt
    64861 catttccata gtccaccatt tacaagtgat caagcaaaag aaaaagttat tgaattgaca
    64921 cgtattttag ctgaacgtgt tggaccaatt aaattgcata ttgtaccatt tacagaattg
    64981 caaaaacagg taaataaagt tgtacatcca agatatacaa tgacttcaac gagacgtatg
    65041 atgatgcgtg ttgctgataa attagtacat caaatagggg ctttagctat tgtaaatggt
    65101 gaaaacctag ggcaggtagc cagtcaaaca cttcatagca tgtatgcaat taataatgta
    65161 acttctactc ctgtattacg tcctttatta acttacgata aagaagaaat tattattaaa
    65221 tcgaaagaaa ttggtacatt tgaaacatct attcaaccat ttgaagattg ttgtacaatt
    65281 ttcaccccta aaaatccagt aaccgaacca aactttgata aggtagtcca atatgaaagt
    65341 gtctttgatt ttgaagagat gattaatcgt gctgttgaaa atattgaaac acttgaaata
    65401 actagtgatt ataaaactat taaagaacag caaacaaacc aattaataaa cgacttttta
    65461 taaataaaat cctagagtaa atttaaacat aaggggatgt taaactatgg atttgaactt
    65521 aacgatgatt ataatcataa ttttatttgg ttttatcgcg gcgtttatag attcggttgt
    65581 agggggtggc ggtttaattt ctacgccagc attattagca atcggtctac caccatctgt
    65641 ggctttaggt acaaataaat tggcaagttc gtttggttct ttaactagta cgataaagtt
    65701 tataaggtcc ggtaaagtgg acttatatgt tgttgccaaa ttatttggtt ttgtattttt
    65761 ggcatctgca tgtggcgcat atattgcaac gatggttccg tcacaaatat tgaaaccttt
    65821 aatcatcatt gcactttcgt cggtgtttat attcacatta cttaaaaaag attggggcaa
    65881 tacacgcacg tttactcaat ttacatttaa gaaagccata atatttgcag cactttttat
    65941 attaatcggc ttttatgatg gatttgtagg tgggggaaca ggttcattta tgctttttgt
    66001 attgttagtc tttggttttg attttttaag tgcagcagga aatgctaaag ttttgaactt
    66061 tgcttctaat ataggtgcgc ttgtattatt tatggtatta ggacaagtag attatgtaat
    66121 aggtttaatt atggctataa gtatgattgc tgggtcatat gctggggctc attttgctat
    66181 taaacaaggt gttggttatg taaaagtatt atttattatt attactgcta ttttaatttt
    66241 aaaaaatgct tttgattata ttcaacaatt tgtataaaat taaatttgct tttgattaaa
    66301 gcatgaaaat tgtaatcaaa ccataaattg tcgtatgatg tagttagaat tttaaaatgc
    66361 aggaggtcaa gtatatgact gaaataacat tcaaaggtgg accaatccac ttaaaaggtc
    66421 aacaaattaa tgaaggtgat tttgcacctg attttacagt gttagataat gacttaaatc
    66481 aagtaacatt agcagattat gctggtaaaa agaaattaat tagtgtggta ccatcaattg
    66541 atacaggtgt ttgtgatcag cagactcgca aattcaactc tgatgcttct aaagaagagg
    66601 ggattgtgct tacaatttca gcagacttac cattcgcaca aaaaagatgg tgcgcttcag
    66661 caggtttaga caatgtcatt acattaagtg accaccgtga cttatcattt ggtgaaaact
    66721 atggcgttgt tatggaagaa cttcgcttat tagctcgtgc agtatttgta ttagatgcag
    66781 ataataaagt tgtttataaa gaaatcgtta gtgaaggtac tgatttccca gattttgatg
    66841 ctgctttagc tgcatacaaa aatatttaat cattaaagag ataaatctta aaatgtatac
    66901 atcgtgtcca tcgttgtcaa cagcattaaa atagaattgt tttctatgat tgctaagacc
    66961 tatgggcact ttttattgga gagggacgaa tatggcagaa caacaaacaa ttatggaacg
    67021 cttgtttcat acattagatg aaaaagctaa aacattaaat aatgaaaatg gccaaagttt
    67081 tattgaaaat cttgggctag caatggaaca agtatatacc aatgaaagag gattgttaga
    67141 acaatcgacg ttacaagatc gacgtaaagc attccaattt gcatatttaa gtttaatgca
    67201 ggaagaaaag atacaagcaa atcatcaaat tacaccagat tcaattggat tgatactagg
    67261 atttttagtt gagcgtttta tgaacaacca agaagaatta catattgttg atattgcaag
    67321 tggtgccggt catttaagtg ctactgtaaa agaagtgtta cctgaaattg cggttatgca
    67381 tcatttaatt gaagttgatc cagttttatc acgtgttagt gtacatttag caaacttctt
    67441 agaaattcct ttcgatgtgt atcctcaaga tgccatcatg ccactaccat tagaagaagc
    67501 agatatcgtt attggtgatt ttccagtagg ctattatcca attgatgaaa gaagtaagga
    67561 gtttaagcta ggttttgaag aaggacatag ttattcacat tatttattaa tagaacaagc
    67621 aataaatgca ttaaaagatg ctggatatgc ctttctagtg gtaccaagta atatttttac
    67681 aggtgaacat gtaaaacagc ttgaaaaata tattgcaaca gagacagaga tgcaagcatt
    67741 tttaaattta ccaccaactt tatttaaaaa tgaaaaagcg cgaaaatcta tattaatttt
    67801 acaaaagaaa aaatcgggtg aaacaaagcc agttgaagta ttattggcaa atattcctga
    67861 tttcaaaatt ccttcacaat ttcaaggatt tatgacagag ttaaatcagt ggatggacac
    67921 aaatcgtcct aaaaaataat ctgtaatagt atagtcataa aactgtatga atgataaaat
    67981 gaaaaatgaa atagtataaa aagcaaacga tggaggatca ttacttatgt caaaattaat
    68041 cttggctatc aatgctggta gttcatcatt aaaatttcaa ttaattagaa tgcctgaaga
    68101 ggaattagta acaaaaggtt tagtagaaag aatcggattg aaggattcaa tttttacaat
    68161 tgaagtcaac ggggaaaaag ttaaaacagt acaagatatc aaggatcacg ttgaggcagt
    68221 tgatatcatg ttggatgctt ttaaagcgca caatattatt aatgatatta acgatatcga
    68281 tggaacaggt caccgtgttg tacatggtgg tgaaaaattc cctgaatcag tagctattac
    68341 tgatgaggtt gaaaaggaaa ttgaagaatt aagcgaatta gcaccgcttc acaacccagc
    68401 taacttgatg ggtattcgtg ctttccgtaa attattacca aatatccctc atgtggcaat
    68461 ttttgataca gcattccatc aaacaatgcc tgaaaaagca tatctatata gcttgccata
    68521 tcattattat aaagattatg gcattcgtaa gtatggtttc catggtacaa gccataaatt
    68581 tgtatcacaa agagcggcag aaatgttaga taagccaatt gaagatttac gtattatttc
    68641 atgtcacatt ggtaatggag catcaattgc agctatcgat ggcggtaaat ctattgatac
    68701 atcaatgggc ttcacaccac ttgctggtgt aacaatgggt actcgttctg gtaatattga
    68761 ccctgcttta attccattta ttatggaaaa aacaggtaaa actgctgaac aagtattgga
    68821 aatcttaaac aaagaatcag gtttattagg attatcaggt acatctagtg atttacgtga
    68881 cttatcagaa gaagctgaat caggtaaagc acgttcacaa atggctttag atgtatttgc
    68941 ttctaaaatt cacaaatata ttggatcata tgctgcgaga atgcatggtg ttgatgttat
    69001 cgtatttaca gcaggtatcg gtgaaaactc agtagaaatt cgtgcgaaag ttcttgaagg
    69061 cttagaattt atgggtgttt actgggatcc taagaaaaat gaaaacttac tacgtggtaa
    69121 agaaggattt attaactatc ctcattcacc agttaaagtt gtcgttattc caacagatga
    69181 agaaagtatg attgcccgtg atgttatgac attcggtggt ttaaaataat ttttaaataa
    69241 atattaagcg tctatctccg ctacaattat atgtaggagg tagacgcttt ttgtattgtt
    69301 tgaaaattaa ctagtgtaat gtctcttttc acgaatatct taaagaatta agtagtcgaa
    69361 gaagattatg aaaacattat taagtgagtt ttatttataa acaataaaaa accaagtgca
    69421 gaaagttgca cttggttcat aatgatcaag tagttatcta atgatcatct atatttaatt
    69481 ttgatatttt tcacgtaatt gagttgttgc aacttgtggt tggaagtctg ctggtaactc
    69541 ttcagtacga acaactaaca cgtcacatgg cgcatgacga acgatagatt ctgatacaga
    69601 accaacaata aatctttcca cggcatttaa gcctgatgta ccactcataa tcaagtctgc
    69661 attaatttca tgtgcaagct ttttaggaat gatagattta ggagagccaa actctagacg
    69721 cgtttctaca tctttaacac cagcgttagt agctacttct ttataaccat ttaataattc
    69781 ttctgcaaaa tgcttagatt tttcagtaaa ttgagcatca taaacttcat aagaagaata
    69841 cgttcttgaa tcaattacat tcacaattgt taacttcgca tcgttacgtt tagcaacacc
    69901 aactgctctg ttaaatgccc attccgcttc atgtgaaccg tcaactgcga ttaaaatatt
    69961 tttgtaagta atcatagtaa gtgcctcctt gttctttctt aattatattt taccacaatt
    70021 ttcggaaaag tcagtgtaat aacctttaca attttgtgga tttttgattg cgctttcaaa
    70081 atgttcgcgt taagatgatt atggaggtgg atggtatgaa aattggtata ccaagggaga
    70141 ttaaaaataa tgaaaatcgt gttggtttat caccaagtgg tgtgcacgct ttagtagaaa
    70201 gtgggcatac tgttttagtg gaaacaaatg cgggttcagg atcattcttt gaagatgtag
    70261 attacaaaga agcaggtgct gagattgttg ctgaacaagc aaaagtttgg gatgtggata
    70321 tggttattaa agttaaagaa ccacttgaat ctgaatatcc atattttaaa gaagggcttg
    70381 tattattcac ttatcttcat ttagcaaatg aagaaaaatt aacacaagct ttgatagata
    70441 gaaaagtaat tagtattgca tatgagactg tgcagttacc agaccgatct ttaccattgt
    70501 tatcaccaat gagtgaggta gcaggaagaa tgtcagctca agttggcgca gagttcctac
    70561 aaaaacttaa tggtggtatg ggaattctac taggtggtgt cccaggagta cctaagggta
    70621 aagtaactat tatcggtggt ggtcaagcag gaacaaatgc agctaaaatt gcactaggac
    70681 taggtgcaga tgttacgatt ttagatgtta atccaaagcg tttacaacaa ttagatgatt
    70741 tattcggtgg acgtgtacat acaattatgt caaatccgtt gaatattgag ttgtatgtta
    70801 aacaaagtga tttagtaata ggtgcagttt taattccagg tgctaaagcg ccaagacttg
    70861 taacagaaga catgattaaa caaatgaaaa atgggtcagt tattattgac attgctattg
    70921 atcaaggcgg tatttttgaa acaactgata aaattacgac acatgatgat cctacatata
    70981 ttaagcatgg tgtggttcat tatgcagttg caaatatgcc aggtgcagta ccgcgtactt
    71041 cgacgttagc tttaaataat gctacgctac cttatgcgct catgctagct aataaagggt
    71101 atagagaagc atttaaatca aatcaaccat tatcattagg tttaaatact tacaaaggtc
    71161 acgtaaccaa taaaggcgtt gcagaggcat ttgaaatgga atataaatct gtagaagaag
    71221 cattacaatt ataatttgaa tttgatgaaa agggaatgga ttacagcgta ctaaagaatg
    71281 acgttatcat ctccttggtt attaaaatta aataatgata aattaaagct agaaacaatg
    71341 cgaagaaatc atcgaacatg aatgatattg atttgattca ttacatgtta ctgatgaaca
    71401 tacctgcata tgtgtttcta gcttgtttgt tacatattgc atttagactt ggataaaaat
    71461 ttatgtctta ggcgctacaa tgttatattg gcagtgtttg actgaatgag aatgcgcttg
    71521 taacaagctt ttcattttta tcccactcct tatttttcgt aatgtgttaa tacttcatat
    71581 ccttcattag tgacaagtat gtcatcttca attcttacac ctgcaacacc aggtacataa
    71641 atacctggtt cgattgtaat aaccatgcca gcttctaaca aattagaatt agtacttgaa
    71701 acatcttgat attcatgttc ttgtaatcct aggccatgac ctaagcgatg agggaaatat
    71761 tcaccataac ctttttctga aataatattt ctagcgatat gatcgatatc ttttaatggt
    71821 attccaggtt taattgcttg gattgcagat gtttctgctt ctaatacaat attataaatt
    71881 tcttgtgctt ctttgctagg ttcaccaaat ttaatagtac gtgtcatatc gctacaataa
    71941 tgctcataaa ttacacctaa atcaaatagt acatattcat tgctttttaa tctgcgatct
    72001 cctggtgtgc catgaggtga tgcggcatga tctccaaata aaaccatcgt atcaaaactc
    72061 atttcattga cgccatattg tttgatagtt tgctcaatat ggttgactac ttcacattca
    72121 gtcacacctt cttttaaata agaaacacct atttcgatac acttatctgc taactcagca
    72181 gctttacgta ttttgctaat ttcatcttcg gatttaatat ttctcaattg tttgattgtt
    72241 aaatcaacat ctccgaatga attgacattg aaaccagaga ttaattgttt ttggcgtgct
    72301 actgttaagt gctcgctttc aattagtaat ttattgattg tttgaggata aagtgaaaaa
    72361 gggttttcag tgtctaaata tccaacgatt tcacctgtga aaggtgatgc tttgacttct
    72421 tcgacttcca tttttggaca aaatagtact tgtttaccat ctttcttaat caataatgca
    72481 aataatcttt catggggttc gctacggtat ccagtaaaat aatatacatt caacggtgtt
    72541 gtaatccatg ctgcatcagc ttgttgattg ttcaattcgt ctattatttt tgatattttt
    72601 gtcattttat aaacactcct caacataata ttacttccat tttatgccaa ttattattag
    72661 atttcaaaat cgagctaata cattaactag attttcaatt tttaaaattt gagaatttgt
    72721 tataattaag tggcaaaagg gtactaaatt tatatagtaa ttgataggag gatgctcaaa
    72781 tgaagttatc atttcatggt caatcaacga tttatttaga agggaacaac aaaaaggtta
    72841 tcgtagatcc gtttatttca aataacccta agtgtgattt gaatattgaa acagtgcagg
    72901 tagactatat cgttttaacg cacggtcatt ttgatcattt tggtgatgtt gtggaactag
    72961 caaagaaaac aggagcaaca gtaattggaa gtgcagaaat ggctgactat ctttcttcat
    73021 atcacggtgt tgaaaatgtt catggtatga atattggtgg taaagcaaac tttgatttcg
    73081 gaagcgttaa atttgttcaa gcatttcata gttcaagttt tacacatgaa aatggtattc
    73141 cagtttattt aggtatgcca atgggtatcg tgtttgaagt tgaaggaaaa acaatttatc
    73201 atactggtga tacaggtctg tttagtgaca tgtcacttat tgcgaaacgt catccagttg
    73261 atgtatgttt tgtaccaatt ggagataatt ttacgatggg tattgatgat gcaagttatg
    73321 ctattaatga atttattaaa ccaaaaattt ctgtaccgat tcattatgat acattcccat
    73381 taatcgaaca agatccacaa caatttaaag atgctgttaa tgtaggggac gtgcaaattt
    73441 taaaaccagg tgaatctgtt cagttttaaa tagaatatag attttacact aaaaaacacc
    73501 tcgctaatct atgtggaagt tatcgtctgc atatttttag tgggtgtttt tatattctac
    73561 tgattcagtt acgacgtgga cgggggacat cagtggttct ttatcatttc tgacctactc
    73621 tcgtaatttt agttttatac aattagacat tgattataaa tttactacga tataaaaaat
    73681 ccagaaccac agtcataatc gtttcttgta tttactcagt cgtttgagac tgcatcatga
    73741 ttgaaagatt atttgtgatt ctggataaaa attttattta acgattaata cagggatagt
    73801 cgcacgtttt gctactttgt ggctgacact tccaagtaca aatttctttt tgtcttctgc
    73861 tttacggtta cttaaaacaa caatttcgta tttaccgcta ttagcatgtt ttaatagttc
    73921 ttcttttgca ttaccacgaa caattatttg gtcataatca ataccataat cttctaaagc
    73981 tttgcgtgtt ttttccaatc gcttgcttcg ttcttcagta agtttgttta aatgaacacc
    74041 tgctttaatt gatgcttgag catcttgttc gctgattgcg tttaaaactg ttacgactgt
    74101 accttcgcca gctaatttag acacttcttt tagtgctttt tcatttttta actgagtgtc
    74161 tacaccaagt aatatatttt tatacattag tacatccctc ctcacttaca ttctaataaa
    74221 taataatgga tttttccatt taaaattaaa gattttgtga tctttgtgtt tgttgtatat
    74281 tttcaaaaaa taaaataggt gaaacgatca ttatttcatt tcacttcaat gctttttatc
    74341 caaaataaag ttataatgta gttaatactg ttcgaaaaga agaggtaaag aatgacaaaa
    74401 catgaacaaa ttttagatta tattgaatcg ttatctatag gctctaaaat ttcggtgcgt
    74461 aaaatcgcta aatttttaaa cgtatctgaa gggacagcat atcgtgcaat taaagatgct
    74521 gataaaatgg gcatggtcgc aacgatagat cgtgttggta cggttcgaat cgaaaaacgt
    74581 aatcgtaatg aaattgaaca tttaactttt aatgaaattg tcaatattat tgatggccaa
    74641 gtgttaggtg gcaacaaagg tatcactaag atggtatcta aatttgcaat tggagcaatg
    74701 gaactcaaag atattttaaa gtatattggt ccaaagacat tattgattgt cggaaatcgt
    74761 gaagatgtgc agattgaagc tttaaaaaga ggtacggcta tattaattac tggtggattt
    74821 aagccatcta ataaagttat taattttgcg aatgaacatg atttacctgt tctatcatca
    74881 agttatgata cattcctagt tgcaaatatt atcaacaaag cactttttaa tcagaaaata
    74941 cgaaaagata tcttaatagt tcaagatata atgacgccat tagatgattt atctgtgctg
    75001 tttgatacga tgaaaatagc agattataag cgtatggcga atcgaactgg ccatacaaga
    75061 tttccggtag tcaatgagtc gtataaatta gtaggtattg taacaagtcg agagatgatt
    75121 aatacaaagg acgacgacga aatagataaa gtgatgacgc gaaatcctat ttatgtaaat
    75181 gctatgagta cagtagcaag ttgtgcgcat atgatgattt gggaaggtat tgaattgatt
    75241 cctgtcgttt cgagtaataa aaagacagtg ggtgtgatta atagacaaga tgttttaaag
    75301 tctatgcaac ttttaggaag acagcctcag atgggtgaaa caattaacga tcaaatcgcc
    75361 aaatatataa caatgaatca agatggaatt acagtagaag tttcaccact tttaatcaat
    75421 cattacggaa cagtaagtaa agcggcattt gtaagtatta tagaagaaac aatacaatat
    75481 gaaatgcgta aatttaaaaa aggtaatgtc atgatagaga acttaaatat cgtgtatatt
    75541 aaaacagtac ctattgaatc acatataaca gtgcgctttg gcatactaga cgtcggaaga
    75601 aacttcgcca aaatagaagt gaacatgcat agtcaaaacg acaaagtagc gagcgctctc
    75661 gtgatatgcc aaatgtttga cgaagtatag gaacttagca gagaaaatag gcataagata
    75721 gatttcgcac aaacgttggg gccccacccc aacttgcatt gtttgtagaa attaggggtc
    75781 caatttctct gtgttggggc ccctgaacta aatagtatga aaatatatat atctaaataa
    75841 ttaaaacggg caaatagttt aatatggtag taacaatttt tataatttag atattttgat
    75901 atagactaat gcttaaatta agcaactttt ctaaaaaagg gagtatgaaa atgattagta
    75961 ctttgaatga aattatgaaa tgtatcgaag acaacgatac aattattata cacagacatg
    76021 ttagaccaga tcctgatgca tatggttcac aacttggttt aaaatactat attcagcaga
    76081 aatttccgca aaagcaagta tttgcagtgg gtgaagcgga atcatcatta agttttattg
    76141 gagagttaga taacattgat gataaaacat atcaagatgc gcttgtaatt gtatgtgata
    76201 ctgccaatgc tccacgaatt gacgatgaac gttatagtac aggtcgtaaa cttattaaaa
    76261 tagatcatca tcctgcagtt gatcagtatg gtgatattaa tttagttaat acgaacgcgt
    76321 catctacaag tgaaatcatt tacgatttaa tctcacattt taatgatgaa gcaattgtta
    76381 ataaagatat cgcgagcgtt ttatatcttg gtatcgtcgg tgatactggg cgattccttt
    76441 ttaacaatac ctcagaacat actatggaaa ttgctggaaa gttaattggg catgatattg
    76501 atcataatgc gcttttaaat aaaatgatgg agaaggaccc aaaaatgttg ccgtttcaag
    76561 gttatgtttt acaacatttc gaacttatgg atgatggatt ctgccaagtt aaaataactg
    76621 aagatgtatt ggagcaattc ggtattcagc caaatgaagc atctcagttt gttaatacaa
    76681 ttgctgacat caaaggcttg aaaatatggg tatttgcagt cgatgaaggt aatgaaatca
    76741 gatgtcgatt acgttctaaa gggcaattga ttattaatga tattgcgcaa gattttggtg
    76801 gcggtggtca tccgaatgcg tcaggagttt cagtggacag ctgggatgaa tttgagcaac
    76861 ttgctacagc tttacgcaca aaacttaact aatagaaagg ggccattcaa tcatggtggc
    76921 atatttaaat attcatacgg cttatgattt gttaaattca agcttaaaaa tagaagatgc
    76981 cgtaagactt gctgtgtctg aaaatgttga tgcacttgcc ataactgaca ccaatgtatt
    77041 gtatggtttt cctaaatttt atgatgcatg tatagcaaat aacattaaac cgatttttgg
    77101 tatgacaata tatgtgacaa atggattaaa tacagtcgaa acagttgttc tagctaaaaa
    77161 taatgatgga ttaaaagatt tgtatcaact atcatcggaa ataaaaatga atgcattaga
    77221 acatgtgtcg tttgaattat taaaacgatt ttctaacaat atgattatca tttttaaaaa
    77281 agtcggtgat caacatcgtg atattgtaca agtgtttgaa acccataatg acacatatat
    77341 ggaccacctt agtatttcga ttcaaggtag aaaacatgtt tggattcaaa atgtttgtta
    77401 ccaaacacgt caagatgccg atacgatttc tgcattagca gctattagag acaatacaaa
    77461 attagactta attcatgatc aagaagattt tggtgcacat tttttaactg aaaaggaaat
    77521 taatcaatta gatattaacc aagaatattt aacgcaggtt gatgttatag ctcaaaagtg
    77581 tgatgcagaa ttaaaatatc atcaatctct acttcctcaa tatgagacac ctaatgatga
    77641 atcagctaaa aaatatttgt ggcgtgtctt agttacacaa ttgaaaaaat tagaacttaa
    77701 ttatgacgtc tatttagagc gattgaaata tgagtataaa gttattacta atatgggttt
    77761 tgaagattat ttcttaatag taagtgattt aatccattat gcgaaaacga atgatgtgat
    77821 ggtaggtcct ggtcgtggtt cttcagctgg ctcactggtc agttatttat tgggaattac
    77881 aacgattgat cctattaaat tcaatctatt atttgaacgt tttttaaacc cagaacgtgt
    77941 aacaatgcct gatattgata ttgactttga agatacacgc cgagaaaggg tcattcagta
    78001 cgtccaagaa aaatatggcg agctacatgt atctggaatt gtgactttcg gtcatctgct
    78061 tgcaagagca gttgctagag atgttggaag aattatgggg tttgatgaag ttacattaaa
    78121 tgaaatttca agtttaatcc cacataaatt aggaattaca cttgatgaag catatcaaat
    78181 tgacgatttt aaaaagtttg tacatcgaaa ccatcgacat gaacgctggt tcagtatttg
    78241 taaaaagtta gaaggtttac caagacatac atctacacat gcggcaggaa ttattattaa
    78301 tgaccatcca ttatatgaat atgccccttt aacgaaaggg gatacaggat tattaacgca
    78361 atggacaatg actgaagccg aacgtattgg gttattaaaa atagattttc tagggttgag
    78421 aaacttatcg attattcatc aaatcttaac acaagtcaaa aaagatttag gtattaatat
    78481 tgatatcgaa aagattccgt ttgatgatca aaaagtgttt gaattgttgt cgcaaggaga
    78541 tacgactggc atattccaat tagagtctga cggtgtaaga agtgtattaa aaaaattaaa
    78601 gccggaacac tttgaagata ttgttgctgt aacttctttg tatagaccag gtccaatgga
    78661 agaaattcca acttacatta caagaagaca tgatccaagc aaagttcaat atttacatcc
    78721 gcatttagaa cctatattaa aaaatactta cggtgttatt atttatcaag agcaaattat
    78781 gcaaatagcg agcacatttg caaacttcag ttatggtgaa gcggatattt taagaagagc
    78841 aatgagtaaa aaaaatagag ctgttcttga aagtgagcgt caacatttta tagaaggtgc
    78901 aaagcaaaat ggttatcacg aagacattag taagcaaata tttgatttga ttctgaaatt
    78961 tgctgattat ggttttccta gagcacatgc tgtcagctat tctaaaattg catacattat
    79021 gagcttttta aaagtccatt atccaaatta tttttacgca aatattttaa gtaatgttat
    79081 tggaagtgag aagaaaactg ctcaaatgat agaagaagca aaaaaacaag gtatcactat
    79141 attgccaccg aacattaacg aaagtcattg gttttataaa ccttcccaag aaggcattta
    79201 tttatcaatt ggtacaatta aaggtgttgg ttatcaaagt gtgaaagtga ttgttgatga
    79261 acgttatcag aacggcaaat ttaaagattt ctttgatttt gctagacgta taccgaagag
    79321 agtcaaaacg agaaagttac ttgaagcact gattttagtg ggagcgtttg atgcttttgg
    79381 taaaacacgt tcaacgttgt tgcaagctat tgatcaagtg ttggatggcg atttaaacat
    79441 tgaacaagat ggttttttat ttgatatttt aacgccaaaa cagatgtatg aagataaaga
    79501 agaattgcct gatgcactta ttagtcagta cgaaaaagaa tatttaggat tttatgtttc
    79561 gcaacaccca gtagataaaa agtttgttgc caaacaatat ttaacgatat ttaaattgag
    79621 taacgcgcag aattataaac ctatattagt acagtttgat aaagttaaac aaattcgaac
    79681 taaaaatggt caaaatatgg cattcgtcac attaaatgat ggcattgaaa ctttagatgg
    79741 tgtgattttc cctaatcagt ttaaaaagta cgaagagttg ttatcacata atgacttgtt
    79801 tatagttagc gggaaatttg accatagaaa gcaacaacgt caactaatta taaatgagat
    79861 tcagacatta gccacttttg aagaacaaaa attagcattt gccaaacaaa ttataattag
    79921 aaataaatca caaatagata tgtttgaaga gatgattaaa gctacgaaag agaatgctaa
    79981 tgatgttgtg ttatcctttt atgatgaaac gattaaacaa atgactactt taggctatat
    80041 taatcaaaaa gatagtatgt ttaataattt tatacaatcc tttaacccta gtgatattag
    80101 gcttatataa cttttatcaa ctaataaatt atgatatagt aaactgatgg ttagatattt
    80161 tttaaccatc agtttgcgtt tatattagtt ttttatgctt attattttta tgagtttcac
    80221 tttacattat tgattaatcc ataagaataa ttagcacaaa aagcagtata cataaattga
    80281 gtaaagaatt ttgtcgatat atcgtgcgga aaaataataa ctttttgata tattggtttt
    80341 tgctatccat tataataatt aaaggtatat ctttagatag tatggtttaa tgtatcttta
    80401 taaatattat tttattatcg tttatcgcta tttgtgttac ataatcaaaa ccataaattc
    80461 ttactcattc agatttaccc aatattttta cttttataat gtaatgcgtt ttatccaagt
    80521 tattttttaa aaataaatat tgaattaggg gctgatttca tgtcattaag agatgaagca
    80581 ttggaaatgc acaaacgtaa tcaaggtaaa ttagaagtta aaccaaatgt aaaagttact
    80641 aataaagagg aattaagttt agcatactca cctggcgttg ctgaaccgtg taaagatatt
    80701 tatgaagata aaagaaaagt atatgattac acaattaaag gaaatacagt tgcagttatt
    80761 actgatggaa cagcggtatt aggtttaggt aacattggac ctgaagcaag tattcctgta
    80821 atggaaggta aagcagtatt attcaaaagc ttcgctggta tcaatggggt gcctattgcg
    80881 ttaaatacaa ctgataccga agaaatcatt aaaacagtta agttgttaga acctaattat
    80941 ggtggtatta atttagagga tatttcggca ccacgttgtt ttgaaattga agaacgattg
    81001 aaaaaagaaa ctaatattcc ggtattccat gacgatcaac atggtacagc aattgtaaca
    81061 ttggcaggtt tggtaaatgc attgagagtt gttaacaaag atattgctaa aataaaagtt
    81121 gtactaaatg gtgctggtgc agcaggaata gccattgtta aattactata cgcgtatggt
    81181 gtaagaaata tggttatgtg tgactcaaga ggcgcaattt ttgaaggacg ttcatatggt
    81241 atgaatccta cgaaagatgt tgtagcaaaa tggacaaata aagataagat tgaagggtct
    81301 ttagaagaag tcgtaaaaga tgcagatgta tttatcgggg tttctgtagc taatgcgctg
    81361 tcacaagata tggttaagag tatggcagat aatccaatta tatttgcaat ggctaatcca
    81421 aatcctgaaa taatacctga tgatgccaaa gcggcaggtg cacgagttgt tggtacagga
    81481 cgttcagact atcctaacca aattaataat gtattagctt tccctggtat ttttagaggt
    81541 gcattagagg ttgaagctac acatataaat gaagaaatga aaaaggcagc tgtagaagcg
    81601 attgctgatt taatcgatag ttctgaatta aatgaagact actgtatccc aggaccgttt
    81661 gataaacgtg tagcgccatc agttgctcgt aatgttgcta aagcggcaat ggaatctgga
    81721 gtagctagga ttgaagttga tccgcaagat gtgtatgata aaacaatgaa acttacagat
    81781 ttacaataaa aataattaac gtttaattaa attgcttaaa agttagcaat aaagagtcat
    81841 taaaatgatg aaaatgaaag tttatgataa acattcaaca gtcaaacgaa tataaatcaa
    81901 ataaatttaa acccgttttt aactggtcaa gttcagttta aggctctaaa tggttagaac
    81961 agaggttatt tggaggtttt cctatgttta aagatttttt taatcgaaca aagaaaaaga
    82021 aatatcttac agtacaagac tctaaaaata atgatgtgcc tgcaggtatt atgactaagt
    82081 gtccaaagtg taagaaaatt atgtacacaa aagaattagc tgaaaattta aatgtgtgct
    82141 ttaattgtga tcatcatatt gctttaactg cgtataaacg tatagaagca atttctgatg
    82201 aaggatcatt tacagaattc gataagggaa tgacctctgc gaatccatta gattttccaa
    82261 gttatttaga aaaaattgaa aaggaccaac aaaagacagg tcttaaagaa gcagttgtga
    82321 ctggtacagc acaactagat ggtatgaaat ttggcgttgc tgtcatggat tcacgtttta
    82381 gaatgggaag tatgggatcg gttatcggtg aaaagatatg tcgcatcatt gattactgca
    82441 ctgagaaccg tttaccattt attcttttct ctgcaagtgg tggtgcacgt atgcaagaag
    82501 gtattatttc cttgatgcaa atgggtaaaa ccagtgtatc tttaaaacgt cattctgacg
    82561 ctggactatt atatatatca tatttaacac atccaactac tggtggtgta tctgcaagtt
    82621 ttgcatcagt tggtgatata aatttaagtg agccaaaagc gttgataggt tttgcaggtc
    82681 gtcgagttat tgaacagaca ataaacgaaa aattgccaga tgatttccaa actgcagaat
    82741 ttttattaga gcatggacaa ttggataaag ttgtacatcg taatgatatg cgtcaaacat
    82801 tgtctgaaat tctaaaaatc catcaagagg tgactaaata atgttagatt ttgaaaaacc
    82861 actttttgaa attcgaaata aaattgaatc tttaaaagaa tctcaagata aaaatgatgt
    82921 ggatttacaa gaagaaattg acatgcttga agcgtcattg gaacgagaaa ctaaaaaaat
    82981 atatacaaat ctaaaaccat gggatcgtgt gcaaattgcg cgtttgcaag aaagacctac
    83041 gaccctagat tatattccat atatctttga ttcgtttatg gaactacatg gtgatcgtaa
    83101 ttttagagat gatccagcaa tgattggtgg tattggcttt ttaaatggtc gtgctgttac
    83161 agttattgga caacaacgtg gaaaagatac aaaagataat atttatcgaa attttggtat
    83221 ggcgcatcca gaaggttatc gaaaagcatt acgtttaatg aaacaagctg aaaaattcaa
    83281 tcgtcctatc tttacattta tagatacaaa aggtgcatat cctggtaaag ctgctgaaga
    83341 acgtggacaa agtgaatcta tcgcaacaaa tttgattgag atggcttcat taaaagtacc
    83401 agttattgcg attgtcattg gtgaaggtgg cagtggaggt gctctaggta ttggtattgc
    83461 caataaagta ttgatgttag agaatagtac ttactctgtt atatctcctg aaggtgcagc
    83521 ggcattatta tggaaagaca gtaatttggc taaaattgca gctgaaacaa tgaaaattac
    83581 tgcccatgat attaagcaat taggtattat agatgatgtc atttctgaac cacttggcgg
    83641 tgcacataaa gatattgaac agcaagcttt agctattaaa tcagcgtttg ttgcacagtt
    83701 agattcactt gagtcattat cacgtgatga aattgctaat gatcgctttg aaaaattcag
    83761 aaatatcggt tcttatatag aataatcaac ttgagcattt ttatgttaaa tcgatactgg
    83821 gttttaccat aaattgaagt acattaaaac aataatttaa tatttagata ctgaatttta
    83881 actaagatta gtagtcaaaa ttgtggctac taatcttttt ttaattaagt taaaataaaa
    83941 ttcaatattt aaaacgttta catcaattca atacattagt tttgatggaa tgacatatca
    84001 atttgtggta atttagagtt aaagataaat cagttataga aaggtatgtc gtcatgaaga
    84061 aaattgcagt tttaactagt ggtggagatt cacctggaat gaatgctgcc gtaagagcag
    84121 ttgttcgtac agcaatttac aatgaaattg aagtttatgg tgtgtatcat ggttaccaag
    84181 gattgttaaa tgatgatatt cataaacttg aattaggatc agttggggat acgattcagc
    84241 gtggaggtac attcttgtat tcagcaagat gtccagagtt taaggagcaa gaagtacgta
    84301 aagttgcaat cgaaaactta cgtaaaagag ggattgaggg ccttgtagtt attggtggtg
    84361 acggtagtta tcgcggtgca caacgcatca gtgaggaatg taaagaaatt caaactatcg
    84421 gtattcctgg tacgattgac aatgatatca atggtactga ttttacaatt ggatttgaca
    84481 cagcattaaa tacgattatt ggcttagtcg acaaaattag agatactgcg tcaagtcacg
    84541 cacgaacatt tatcattgaa gcaatgggcc gtgattgtgg agatctagca ttatgggctg
    84601 gattatcagt tggtgctgag acaattgtag ttccagaagt gaaaacagat attaaagaaa
    84661 tagctgataa aattgaacaa ggtattaaac gtggtaagaa acactcaatc gttcttgtag
    84721 cagaaggttg tatgactgcg caagattgtc aaaaagaatt atcacaatac atcaatgttg
    84781 ataatagagt gtctgtgtta ggtcacgttc aacgtggtgg tagcccaaca ggtgcggata
    84841 gagttttagc atcacgttta ggtggatatg cggtagactt attaatgcaa ggtgaaacag
    84901 ctaagggtgt tggaattaag aacaataaaa ttgtagcaac atcttttgat gaaatttttg
    84961 atggtaaaga tcataaattt gattatagtc tatatgaact tgctaacaag ttatctatat
    85021 aagatttcag gaggaattat aaaatgagaa aaactaaaat tgtatgtaca attggaccag
    85081 cttcagaatc agaagaaatg attgagaaat taatcaatgc tggtatgaac gttgcacgat
    85141 taaacttttc acatggtagt catgaagagc ataaaggtag aattgataca attcgtaaag
    85201 tagctaaaag attagacaaa attgtagcaa ttttattaga tacaaaaggt ccagaaattc
    85261 gtacgcataa tatgaaagac ggtatcattg aacttgaacg tggcaacgaa gttattgtta
    85321 gcatgaatga agttgaagga acacctgaaa agttctcagt aacatatgaa aacttaatta
    85381 acgatgttca agtaggttca tacattttac ttgatgatgg cttaattgaa ttacaagtta
    85441 aagatattga ccatgctaaa aaagaagtta aatgtgatat tttaaactct ggtgagctta
    85501 aaaacaaaaa aggtgttaac ttacctggcg taagagtaag tttacctggt attacagaaa
    85561 aagatgctga agatatccgt ttcggtatta aagaaaatgt tgacttcatt gcagcaagtt
    85621 tcgtacgtcg tcctagtgat gttttagaaa ttcgtgaaat tttagaagaa caaaaagcta
    85681 acatttcagt attccctaaa attgaaaacc aagaaggtat tgataatatt gcggaaattc
    85741 ttgaagtgtc tgatggttta atggttgcac gtggtgacat gggtgttgaa attccacctg
    85801 aaaaagtacc aatggttcaa aaagatttaa tcagacaatg taacaaatta ggtaaaccag
    85861 ttattacagc tacacaaatg ttagattcta tgcaacgtaa cccacgtgct acacgtgcag
    85921 aagctagtga cgttgccaac gcaatctatg atggtacaga tgcagtaatg ttatctggtg
    85981 aaactgctgc tggtttatat cctgaagaag ctgttaaaac aatgagaaat attgctgtat
    86041 cagctgaagc agcccaagat tacaaaaagt tattgtcaga tcgtactaaa ttagttgaaa
    86101 cttcattagt gaatgctatc ggtatttcgg ttgcacatac agctttaaac ttaaatgtta
    86161 aagcaattgt agctgctact gaaagtggtt caacggcacg tactatctcc aaatatcgtc
    86221 cacattcaga cattattgcg gtgactccaa gtgaagaaac tgcacgtcaa tgttcaattg
    86281 tttggggagt tcaacctgta gttaaaaaag gacgtaagag tacagatgca ttgttaaaca
    86341 atgcagttgc aacagctgtt gaaactggta gagtatctaa tggtgattta atcattatta
    86401 ctgctggtgt accaactggt gaaactggaa ctactaatat gatgaaaatc cacctagttg
    86461 gtgacgaaat tgctaatggt caaggtattg gacgtggatc agttgttggt actacgttag
    86521 ttgctgaaac tgttaaagat ttagaaggta aagatttatc tgacaaagtt atcgttacta
    86581 actcaatcga tgaaacgttt gtaccttatg tagaaaaagc tttaggctta attacagaag
    86641 aaaatggtat tacatcacca agtgcaattg ttggtttaga aaaaggtatt ccaacagttg
    86701 taggtgtaga aaaagctgtt aaaaacataa gcaataacat gttagttacg attgatgctg
    86761 ctcaaggtaa aatctttgaa ggatatgcaa acgtactata atttataaaa aaacgtcttt
    86821 ccatttatca acaatggaaa ggcgtttttt ggttcatctg gtattttatg acgtaattaa
    86881 taggttattt gataatgata gtgtatgaat ggcaatctat ataaatgttt atatctttta
    86941 tacatgtaca ttatcacctt caaactttca ctcatattac tttggaaatt tattataaaa
    87001 tagaagtatg gatgtatttc tgaaatgata cattattaaa tagatgagaa agtaaaagtt
    87061 ttgagccaag tacgcaattt aatattataa gttgcatata aaacaggatg
//
LOCUS       DAKTGS010000002        69461 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.002, whole genome shotgun sequence.
ACCESSION   DAKTGS010000002 DAKTGS010000000
VERSION     DAKTGS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 69461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.002"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(95..559)
                     /gene="bstA"
                     /locus_tag="O0286_000071"
     CDS             complement(95..559)
                     /gene="bstA"
                     /locus_tag="O0286_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCY0168476.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            726..1064
                     /locus_tag="O0286_000072"
     CDS             726..1064
                     /locus_tag="O0286_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCY0168477.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            1054..1821
                     /locus_tag="O0286_000073"
     CDS             1054..1821
                     /locus_tag="O0286_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168478.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(1940..2578)
                     /gene="pcp"
                     /locus_tag="O0286_000074"
     CDS             complement(1940..2578)
                     /gene="pcp"
                     /locus_tag="O0286_000074"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCY0168479.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(2730..3686)
                     /locus_tag="O0286_000075"
     CDS             complement(2730..3686)
                     /locus_tag="O0286_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCY0168480.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            3930..4901
                     /locus_tag="O0286_000076"
     CDS             3930..4901
                     /locus_tag="O0286_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCY0168481.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(5050..5565)
                     /locus_tag="O0286_000077"
     CDS             complement(5050..5565)
                     /locus_tag="O0286_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCY0168482.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            5945..6793
                     /locus_tag="O0286_000078"
     CDS             5945..6793
                     /locus_tag="O0286_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCY0168483.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            6822..7376
                     /locus_tag="O0286_000079"
     CDS             6822..7376
                     /locus_tag="O0286_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCY0168484.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            7429..9141
                     /locus_tag="O0286_000080"
     CDS             7429..9141
                     /locus_tag="O0286_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0168485.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            9138..9971
                     /locus_tag="O0286_000081"
     CDS             9138..9971
                     /locus_tag="O0286_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0168486.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            10018..10515
                     /locus_tag="O0286_000082"
     CDS             10018..10515
                     /locus_tag="O0286_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0168487.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            10633..11757
                     /locus_tag="O0286_000083"
     CDS             10633..11757
                     /locus_tag="O0286_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCY0168488.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            12062..12880
                     /locus_tag="O0286_000084"
     CDS             12062..12880
                     /locus_tag="O0286_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCY0168489.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            12898..13512
                     /locus_tag="O0286_000085"
     CDS             12898..13512
                     /locus_tag="O0286_000085"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCY0168490.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            13505..14755
                     /locus_tag="O0286_000086"
     CDS             13505..14755
                     /locus_tag="O0286_000086"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCY0168491.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            14771..15784
                     /locus_tag="O0286_000087"
     CDS             14771..15784
                     /locus_tag="O0286_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCY0168492.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            15753..16331
                     /gene="hisB"
                     /locus_tag="O0286_000088"
     CDS             15753..16331
                     /gene="hisB"
                     /locus_tag="O0286_000088"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCY0168493.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            16328..16906
                     /gene="hisH"
                     /locus_tag="O0286_000089"
     CDS             16328..16906
                     /gene="hisH"
                     /locus_tag="O0286_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCY0168494.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            16899..17603
                     /locus_tag="O0286_000090"
     CDS             16899..17603
                     /locus_tag="O0286_000090"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCY0168495.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            17600..18358
                     /gene="hisF"
                     /locus_tag="O0286_000091"
     CDS             17600..18358
                     /gene="hisF"
                     /locus_tag="O0286_000091"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCY0168496.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            18355..18987
                     /locus_tag="O0286_000092"
     CDS             18355..18987
                     /locus_tag="O0286_000092"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCY0168497.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            19180..19359
                     /locus_tag="O0286_000093"
     CDS             19180..19359
                     /locus_tag="O0286_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168498.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
     gene            20080..22122
                     /gene="lip1"
                     /locus_tag="O0286_000094"
     CDS             20080..22122
                     /gene="lip1"
                     /locus_tag="O0286_000094"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCY0168499.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
     gene            complement(22569..23621)
                     /gene="icaC"
                     /locus_tag="O0286_000095"
     CDS             complement(22569..23621)
                     /gene="icaC"
                     /locus_tag="O0286_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCY0168500.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(23608..24480)
                     /gene="icaB"
                     /locus_tag="O0286_000096"
     CDS             complement(23608..24480)
                     /gene="icaB"
                     /locus_tag="O0286_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCY0168501.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            complement(24477..24782)
                     /gene="icaD"
                     /locus_tag="O0286_000097"
     CDS             complement(24477..24782)
                     /gene="icaD"
                     /locus_tag="O0286_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCY0168502.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            complement(24746..25984)
                     /gene="pgaC"
                     /locus_tag="O0286_000098"
     CDS             complement(24746..25984)
                     /gene="pgaC"
                     /locus_tag="O0286_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCY0168503.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            26148..26708
                     /gene="icaR"
                     /locus_tag="O0286_000099"
     CDS             26148..26708
                     /gene="icaR"
                     /locus_tag="O0286_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCY0168504.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            27556..28218
                     /locus_tag="O0286_000100"
     CDS             27556..28218
                     /locus_tag="O0286_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCY0168505.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            28235..28927
                     /locus_tag="O0286_000101"
     CDS             28235..28927
                     /locus_tag="O0286_000101"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCY0168506.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            28924..29691
                     /locus_tag="O0286_000102"
     CDS             28924..29691
                     /locus_tag="O0286_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCY0168507.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            29926..30426
                     /locus_tag="O0286_000103"
     CDS             29926..30426
                     /locus_tag="O0286_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0168508.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            30429..30905
                     /locus_tag="O0286_000104"
     CDS             30429..30905
                     /locus_tag="O0286_000104"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCY0168509.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            30984..31595
                     /locus_tag="O0286_000105"
     CDS             30984..31595
                     /locus_tag="O0286_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0168510.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(31680..32039)
                     /locus_tag="O0286_000106"
     CDS             complement(31680..32039)
                     /locus_tag="O0286_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168511.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(32193..32495)
                     /locus_tag="O0286_000107"
     CDS             complement(32193..32495)
                     /locus_tag="O0286_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168512.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            complement(32482..32940)
                     /locus_tag="O0286_000108"
                     /pseudo
     CDS             complement(32482..32940)
                     /locus_tag="O0286_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33176..33868
                     /locus_tag="O0286_000109"
     CDS             33176..33868
                     /locus_tag="O0286_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0168513.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            34413..41228
                     /gene="sasA"
                     /locus_tag="O0286_000110"
     CDS             34413..41228
                     /gene="sasA"
                     /locus_tag="O0286_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCY0168514.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
     gene            41358..42569
                     /gene="secY2"
                     /locus_tag="O0286_000111"
     CDS             41358..42569
                     /gene="secY2"
                     /locus_tag="O0286_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCY0168515.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            42580..44133
                     /gene="asp1"
                     /locus_tag="O0286_000112"
     CDS             42580..44133
                     /gene="asp1"
                     /locus_tag="O0286_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCY0168516.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            44123..45691
                     /gene="asp2"
                     /locus_tag="O0286_000113"
     CDS             44123..45691
                     /gene="asp2"
                     /locus_tag="O0286_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCY0168517.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            45669..46628
                     /gene="asp3"
                     /locus_tag="O0286_000114"
     CDS             45669..46628
                     /gene="asp3"
                     /locus_tag="O0286_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCY0168518.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            46618..49008
                     /gene="secA2"
                     /locus_tag="O0286_000115"
     CDS             46618..49008
                     /gene="secA2"
                     /locus_tag="O0286_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCY0168519.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            49026..50534
                     /gene="gtfA"
                     /locus_tag="O0286_000116"
     CDS             49026..50534
                     /gene="gtfA"
                     /locus_tag="O0286_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCY0168520.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            50527..51885
                     /gene="gtfB"
                     /locus_tag="O0286_000117"
     CDS             50527..51885
                     /gene="gtfB"
                     /locus_tag="O0286_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCY0168521.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            52127..54034
                     /gene="sasF"
                     /locus_tag="O0286_000118"
     CDS             52127..54034
                     /gene="sasF"
                     /locus_tag="O0286_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCY0168522.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            54202..54762
                     /locus_tag="O0286_000119"
     CDS             54202..54762
                     /locus_tag="O0286_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0168523.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(55029..56888)
                     /locus_tag="O0286_000120"
     CDS             complement(55029..56888)
                     /locus_tag="O0286_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCY0168524.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            57099..60080
                     /locus_tag="O0286_000121"
     CDS             57099..60080
                     /locus_tag="O0286_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0168525.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(60192..61130)
                     /gene="manA"
                     /locus_tag="O0286_000122"
     CDS             complement(60192..61130)
                     /gene="manA"
                     /locus_tag="O0286_000122"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCY0168526.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(61144..63096)
                     /locus_tag="O0286_000123"
     CDS             complement(61144..63096)
                     /locus_tag="O0286_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCY0168527.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(63173..65047)
                     /locus_tag="O0286_000124"
     CDS             complement(63173..65047)
                     /locus_tag="O0286_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0168528.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(65287..65745)
                     /locus_tag="O0286_000125"
     CDS             complement(65287..65745)
                     /locus_tag="O0286_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168529.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            66003..66530
                     /gene="isaB"
                     /locus_tag="O0286_000126"
     CDS             66003..66530
                     /gene="isaB"
                     /locus_tag="O0286_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCY0168530.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            66954..68483
                     /gene="aur"
                     /locus_tag="O0286_000127"
     CDS             66954..68483
                     /gene="aur"
                     /locus_tag="O0286_000127"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCY0168531.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            68850..69299
                     /locus_tag="O0286_000128"
     CDS             68850..69299
                     /locus_tag="O0286_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCY0168532.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            69302..>69461
                     /locus_tag="O0286_000129"
     CDS             69302..>69461
                     /locus_tag="O0286_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168533.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGV"
BASE COUNT        24550 a        10474 c        12678 g        21759 t
ORIGIN      
        1 ctggtataga tggtgtttat taaattcaaa aaagtgacct tttaaacaaa taaaaaaccc
       61 gttaacgtgt tgttaacggt taataagcac atatttattt taaatttttt aatatacgag
      121 acatctcttc aattttacca gcatgaagtg ggatgtgaat ggcatttaat actaataact
      181 cttctaagtt attacaaccg acaattggct gtttcaattc aattgctaaa tcatcttcag
      241 ttagatttcg tgcacgttca ggtaaagttt ggagaccttc taaaatcctt tcaatactcg
      301 gtacttcatc cttccattca tctggagacg aaccatttcc gaataaaggt ctatagatat
      361 ttaaatcaat attctcttta ccagcaacag ctaaggccga ttcaaaaatt gttaatacat
      421 gaccaaattg ccaatgaatg gtattagaaa aaggcttatg ataatcatca agtacctttt
      481 caactttcca agcatcatac acatttacta aatatcctaa ccccatatca atgacatcaa
      541 atactgtttt tgtagtcatt attaattcct cccccataaa taaaatatac ttccgtaagt
      601 attaccctat taacaaagtt gttagtcatt taaggaatgt gtacaacaac taccttacaa
      661 taaaagatag ttgacgaaaa gttctcaatg tactatctta ttatgtaaga tagtaggtga
      721 ttaaaatgtc agtgtcagat cagattatga aaggtctctt agatggggcg atattaggtc
      781 tcattgccca aggcgaaacg tatggatatg aaattatgac gaaacttaag aatcaagagt
      841 ttccagagat aagtgagggt agtatttatc cagttttaat gcgtttaaac aaaaaaggat
      901 acgttgatac gacaataaaa aaatctagta atggcggccc gaggcggaaa tactatacga
      961 ttaccgattc aggtttaaag gaactaaaag tatttaaatc gaaatggcaa gttttgaata
     1021 aagggatgat taacttgttt ggaggggaaa acgatgttaa gtaaacaatc tcaagatttt
     1081 ttagaaaaat tacgtgttga attgttattt agaggtaaga ctgaagcaga agtagaagag
     1141 ttattggaag agctcgaaga tcatttaact atggctgaaa aaaataacga agatacaagt
     1201 tcaattatta atacaccgat taagtcaatg gctgatcaat tatcacctga gataagtctt
     1261 actaccggtt tgtataaata tattactttg tacttcgctt ttattttagc agtcattata
     1321 attcctaggt ttttagattt gggtacattt gatgtgacaa ttgcattttt actttatata
     1381 gctagtatcg ttattttagg tgttgtcatt ggtatgctga ttctacgaaa tacattagtc
     1441 cgatttggtg accaaaaaat aacgtatgtt ataaatgtga cttatggaat tatcgtattt
     1501 ttatggatga ttttaggtgg cttgattatt aaaaaatatc ctatctatag tttctttgag
     1561 ttaagtgaca aacagagttt catcgttggg ttagtacttt taattgcgct cattggaact
     1621 tgtattttaa ttaaacagaa aatttatgcg ctcatcgtgt tggtaatttc attgccaagt
     1681 ttgatagcaa ggatagcagc tatctttagt aaagaaccag acacttttaa tttagtttca
     1741 gtaagtatac tcatcattct aaatatagca tttattattt atacatttgt aaattttagg
     1801 aagttaaaaa aagaagacta aacaatgtcg tttagctaaa atacatcatt atagtaaaac
     1861 tgtataccaa tcatccaaaa gcaccctaaa gttagactta aactaacaaa ttagggtgct
     1921 tttgtttgtg cacttataac tattccgttg tgcccagggc taggtgtaaa tcctcatcat
     1981 tagaaatagc ttcaatggct gcagtcaaac ctgcaactat cttttctaat ggcatagatg
     2041 gtgtatcggg tttaccaatg acctgctctg gtatgtatgg cacatgaata aatccgaatc
     2101 gtaggtgagg gtaatgctta tcttgtaaat aacctaagtg ataaagtgtg tgattacaaa
     2161 caaatgtacc tgcgctattt gaaagtgctc caggaagtcc ttgattaata atactttgag
     2221 tcattgcttt aactggtaaa tttgaaaaat aagctggcgc accgtctaag tgaatggctt
     2281 gatcaatagg ttgaaaatca tcattatctg gaatacgtgc atcatcaata ttaatggcga
     2341 cacgttctgg ggtaatggca tttctaccac cagcttgtcc tatagctagt acaacatcat
     2401 aatgattaga tgccaacgtt ttatttataa tattatctac tttcttaaaa gaggttggta
     2461 gttttaattt atcgattgta tgtgtgccaa taatatcttc tagttgagtc acagcttccc
     2521 atgagggatt gatattttga ttgtcaaaag gcgcaaaccc tgttactaaa atgtgcattt
     2581 taagacaccc cgcagtatta aaatttaatt tagtataaca cgcgcataat taaaaacata
     2641 tcaggttatg tatgcgcata aaaaacagcc ttgaaaaacc tttataaatc ggattttcaa
     2701 agcgtgtttt gaaaggtatt taattaaaac taagcatgtt gatgtaaatc atcaaagttt
     2761 gttaaacgtt gttgccactc attatcacta atattatttg cttgaacata acgattacgc
     2821 tcatgtttag cacattcata agagcatgca cctaaatatt tagtttcgtt ttcttcagaa
     2881 actaatattt gtttattaca ttctgggtta gcgcaattaa tataacgttc acatggtttg
     2941 ccatcaaacc aatccttacc aataattgtt ttttcaactt ggttgatatc aacactgata
     3001 cggtcatcaa atacgtacat tttaccgtcc caatattcac cttttgtttc aggatcttta
     3061 ccatatgtag caataccgcc atgaagttga gctacatctt cgaaaccttc ttttaaaagc
     3121 catccagaaa atttttcgca tcgaatgcca ccagtacagt acgtaaccac ttttttatct
     3181 gcaaataacg ctttattctc tttaatccag tcaggcaaat ctctaaaacg tgtgatattt
     3241 ggacgaattg cacctcggaa atgacctaaa tcaaattcat aatcattacg tgcatcaata
     3301 atgactgtgt catcatcttc aagagctttt ctaaattcta caggtgataa atattggcca
     3361 gttgtgtgtc ttggatcgac gtcatcttct aaatctaaag caacgatttc ttttcgagga
     3421 cgtacatgca ttttcttaaa agcatgtcct tcagcttcat caattttaaa caccatatct
     3481 ttgaatcgtt catcggcatg catatgtgcc atatattgtt cggtttcttc ttttgtacca
     3541 gataatgtgc cgttaatacc ttctgtagaa acaagaattc taccttttaa atggtgtgct
     3601 ttacaaaagg ctaagtgatc ctgagcaaac tgttcagggt catcaatcgt catatattta
     3661 taatataaaa gaacttgata gttcataggt taatctcctt ctataaacat gttataagca
     3721 tgttaatttt taaaataaag cgtcaatacg catatttgtc tacgtccatc attttaacag
     3781 agttgtattg tcattcaata tttctagctt gaaatcagcc actagaccga taaaatagat
     3841 agatgaaacc gttatcattt tatttgatag ttatcttgtt taaaataaaa ttgatagtta
     3901 aaacgaatga aatgaaagga atgatcatga tgtcacaaca agatttacct acattatttt
     3961 atagcgggaa gtccaatagt gctgttccaa ttatatctga aagtgaatta caaacaatta
     4021 cagctgaacc atggcttgaa atttccaaaa aaggattgca actagaagga ttgaactttg
     4081 atcggcaggg acaactcttt ttattggatg tattcgaagg caatattttc aaaatcaatc
     4141 ctgaaacgaa ggaaatcaaa cgaccttttg taagtcacaa agcgaatcct gcagcaatca
     4201 aaatacataa agatggccga ttattcgttt gttatttagg agattttaaa tctacaggag
     4261 gcatttttgc agctacagaa aatggtgaca acttacaaga tattattgaa gatctttcaa
     4321 cagcatattg tattgatgac atggtatttg attctaaagg tggattttat tttacagatt
     4381 ttagaggata ctctaccaat ccactaggag gcgtttatta tgtttcgccg gactttagaa
     4441 cagtgacgcc tatcattcaa aatattagcg tagcaaatgg tattgcttta agtacagatg
     4501 aaaaagtact atgggtaaca gaaacaacag ccaatcgatt acatcgcatt gcacttgaag
     4561 atgatggtgt gacgatacaa ccatttggag ctaccatacc gtactatttt acaggtcatg
     4621 aaggaccaga ctcatgttgt attgatagtg acgataattt atacgtagca atgtatggtc
     4681 aaggtcgagt gttagttttt aataaaaggg gttatccaat aggacaaata ttgataccag
     4741 gccgagatga agggcatatg ttacgttcta ctcatccgca atttatacct ggaacaaatc
     4801 aactcatcat ttgttccaat gatatagaaa tgggcggagg atctatgctt tatacagtta
     4861 atggctttgc aaaaggtcat caaagttttc agtttcaata agacctacct gaatgaaagg
     4921 cgtctaaaat tagggtgaac agttagtgct tgttacacat gagttaaacg aaaaatgcca
     4981 actataccta gggatgatat agttggcatt tcgtatatat attaaaacaa tgaggattgt
     5041 gtaatgagat tattctgaga ttgagaattc agctgatgct tcgaatttaa catctttgcc
     5101 tagcattacg ccaccagttt caagtgcttg gttaaagtta atgccatatt tttctctatt
     5161 gattgtacca gtaacaataa tacctgttac ttgagaacca tccataggat tcttacttac
     5221 tccgttgaat tcaacatcga atgtttcttc gttagtgatg cctttaattg ttaaatcacc
     5281 aacaactttg ctttcagata ctgattttgt cacaaatgta attttatcaa attcgtcagt
     5341 accaaagaaa tcaccagatt ttaagtggtt atcacgtgct tcgtttttag tgttaattga
     5401 gcttggaata attgttgcag tagctttcaa agtactgaag tcattaatat ctccttcaac
     5461 agctacatca aattgatcaa atgaaccttt cactttagaa accattaaat gtttaatttg
     5521 gaattctaaa ctactgtgtg caccatcaaa agtaaagtta gtcataatat aaaacctcca
     5581 tatttttatc tcttagatat attttttata cttgatatac atacaatacc acctaaaaaa
     5641 caaagatgca agcattttat ttcgaattcg aatattttct gtgatgtatt taaagaatga
     5701 cctataaaac accacactat tgttataccc tagtcaatta attcaaacat aaagaagtga
     5761 ttacaagtcc gtgaaattta gttaatgaga taaatctgac tattattatc atttttatag
     5821 tcactactat tgactttgca tcaagtgact cgtactattt taatataatt ctgaaaattc
     5881 aaaatataga acattaaata tgtagataat atttttttga agttttaaaa aaaggaggca
     5941 ttttatggga aattatttgg tattgcaatc agattttgga cttagtgatg gcgctgtaag
     6001 tgcaatgtat ggagttgcat atactgtaag tgatgatata cgtgttgaaa acttaacgca
     6061 tgatatacca ccgtatgaca tttgggtagc gtcatatcgt ttatatcaaa ctgtaaaata
     6121 ctggcctaaa ggcacagttt ttgtatcagt ggtagacccg ggtgtaggta gtgataggcg
     6181 gagcattgct tgcttaacat attcaggtca ttacattatt acacctgata atggttcttt
     6241 aagtcatatt aaacattacg aaggtattaa aaaagttatc gaaattgatg aagtgaaaag
     6301 ccgattgcct cattcggaag aaagtcatac ttttcatggt agagatgttt atgcatacaa
     6361 tggtgcgaga ttagctgcaa ataagatagc gtttgaacgt ctcggtcagg caattaatgt
     6421 tgatagtatt gaggcattag aaataagaga agtgacaaaa aatgaggatt ctgttacagg
     6481 aagcattgat attttagata tcagatttgg gtcattatgg acaaatattc cattggcatt
     6541 cttaaaatcc ttggaaattg ttcatggtaa taatttggtg gtaacaattt atcatcaaga
     6601 taagaaagtc tatcaaaata ttattaaatt tgcgcgatcg tttgcagatg ttaatgtggg
     6661 tgaacctctt gtgtatgtta actcgctagt aaatattggt gtagcagtca atcaagactc
     6721 attttcagat ttatatcata ttggtactgg aaatgattgg acgattcatt tatctaaagc
     6781 accatcaata taatgaattt tttatgtaag gagtaatgaa gatgaaaaaa caagatattt
     6841 cagttaaaac tgttgttgca attggtatag gagcagcggt atttgtaatt ttagggcgtt
     6901 ttgttgtaat accaacaggt tttcctaata cgaatataga aacatcatat gcatttttag
     6961 cattaatatc tgcaatattt ggaccttttg ctggtttaat gactggatta gttggacatg
     7021 ctattaaaga ttttacgaca tacggtagtg cctggtggag ttgggttatt tgctcgggaa
     7081 ttataggctg tttatatggc tggattggac taaaattaaa tctttcttca ggccgatttt
     7141 ctaggaaatc gatggtttat tttaatatcg gtcaaattat tgcgaatatt atttgctggg
     7201 cacttattgc accaacatta gatattttga tttataacga accagctaac aaggtttata
     7261 cacaaggtgt tatctctgca gtattaaata ttatttcagt tggcattatt gggacaatat
     7321 tattaaaagc atatgcttca tctcaaatta aaaaaggtag tttacgtaag gaatagttat
     7381 tttgttgaat cagatatgta aatgaatata gaaaggtaat gatatatcat gactgaacca
     7441 attatctcgt tcaaagactt tagttttcaa tatcatagtc aagcaacacc tacattacag
     7501 aatataaatg ttgatattta tccaggagaa aaagtattag tagttggtgc ttcgggtagt
     7561 ggtaaatcga cttttgcaaa ttgcataaac ggattaattc catttaaaac taaaggtaac
     7621 ataactgggg aactatatat aaataatcaa gatgcaaccg ttagttgttt acatgataga
     7681 tctaatgttg ttggtacagt tttaaaagat acagatggac agttcatagg cttaacagca
     7741 gctgaagata tggccttttt attagaaaat aattgtgttg aacaagatga tatgaagaaa
     7801 aatgtaagtt attgggctga aaaagttggc atgatagaac atttaaatca ccgaccgcaa
     7861 gatttatctg gaggtcaaaa acaacgcgtt tcattaggtg gtatattaat ccatcgtacg
     7921 cctattttaa tattggatga gccactggcc aatttagatc ctgcgacagg acatgaaacg
     7981 ctgagattgt taaacaatat tcatgaagaa acaaagtcaa cgatgattat tgtcgaacat
     8041 cgattagaag aatcattaga tgatacgttt gatagagttc ttctttttaa agatggaaaa
     8101 atcatcgcta atacaacgcc tagtgatttg ctaaaatcat ctaagcttaa agaagcaggc
     8161 atacgtgaac cattatactg tacagcgctt aaatatgcag aagtagatgt cgaatccatt
     8221 gataacttag cgaatttacg tgacgtttgt atgagtgaac atgttaaatt taaagtgaaa
     8281 aaatggatag atgaaacatc tgcaaataat gataataagt acaaatctga accgctatta
     8341 gaattaaatg aggtatgtgt tcaatatagt gactatagta atagtgtact taataatgtt
     8401 caattaaatg tttatagaag agaaatgctt agtatagttg gtcacaatgg tgccggcaaa
     8461 tcaacacttg ctaaagcaat ttgtggattt ttagatataa caggtaatat ccaattttgt
     8521 aataggggat ttaatcaatt gtcaattagc gaacgatctg aattcgtagg ttatgtgatg
     8581 caaaatccaa atcatatgat ttctgaaaaa atgatttacg atgaagtagc attagggtta
     8641 agagcacgag gtatgaaaga atcagatata aagatacgag tagagaatgt gctcaaaata
     8701 tgcggacttt atgcatttcg gaattggcca attgcagctt taagctatgg tcaaaaaaag
     8761 agggtcacta tagcatctgt tttagtctta aatccggaaa taatcatatt ggatgaaccg
     8821 actgctggtc aagatttcta tcattataat gagataatgt catttttaat tgaactaaac
     8881 agacagggga agacgattat tatgattacg catgatatgc atttattgtc tgagtatagt
     8941 tcaagaacag ttgtattatc aaaaggtcaa gtcgttgctg ataccacgcc agtattggtt
     9001 ttaaatgata aaaaaatctg tgagattgca tcattgagac aaacatcgct atttgaaatg
     9061 gccgaatata tagggattag cgagccacag aaattagtac aattatttat taaccatgat
     9121 aggaaggtga gacgccaatg aatcaatata atactatagg ttttcacccg ggaaatagtc
     9181 gtattcatca attaaatgcg actgttaaac ttttattctt attagttgtt tctatttctg
     9241 caatggtgac ttatgacaca agatatttaa ttttaattag tgcttcatct attttattgg
     9301 tcaaatatgc tcatattgaa tggaaacaag ttcgctttgt tgttaaattc attctgtttt
     9361 tcacaatatt aaatattatt gccgtgtaca tatttgaccc tgaatatggt gtgaagattt
     9421 ataatcagcg tacagagtta gtcaatggta ttggtcgatt tacgctaaca tcacaggaat
     9481 tattctatct ttttaatcta atattaaaat atattagtac agttccttta gcgttaatat
     9541 ttttattcac aacgaatccg agtcattttg ctgcaagttt aaatcagcta ggtgtgaatt
     9601 ataaaatcag ttacgcagtc tcactagcat taaggtatat tccagatatt caagaaacat
     9661 attttaatat ttcacaagcg caacaagcaa gaggatatga taattcgaaa aaagcaaaat
     9721 ttactagccg tgttaaaggt atcaaacgca ttgtgttacc tttaatattt tctagtatcg
     9781 aaagaattga cactattagt actgctatgg agttaagaca attcggacag tataaaagga
     9841 gaacctggta cgtcaaaaaa caattaaaaa aagatgatta tgttgttttg tgtttgacgt
     9901 taatacttct gatgttagta gttacattat tctttttaaa taatagtcga tatttcaacc
     9961 cgtggcatta gtattcatat aaatagtctt taaatagaaa taggagggag acatttaatg
    10021 ataaatactg aaagattaaa tttaatgatt ccaagttcct cgcatttaat tgaactttat
    10081 aatatttgta gtcatccaca agcaaatata tacactccca aaggtttaca taattccaaa
    10141 ttagacacac aacggtggat tgaaaaatgg cgaaaccatt ggcaacaata tcaatttggt
    10201 tactttgtat tggtaaaaaa aatagattgt agtgttattg gtatttgtgg atatgaatat
    10261 cgacaattaa agcaagaaac agtattaaat ttattttata aattacatcc aagttttgaa
    10321 ggacaagggt acgcatgtga ggctattaca gcaatcacaa attttgtgaa ttatatcgat
    10381 caagaaacag taaaagttat caggacaaat aagtgtaacc aacgttcaat aaatttagca
    10441 gaaaggctta aattcaagcg agacgatact atggacgaca ttatcaatca aggagatatt
    10501 gtgttttata aataaaatac tatgacatta tctaaaaaat aaaattaaaa tcccaacagt
    10561 ttcttaaaaa gttatgagcc acataataaa aatgttataa ttgtgcataa taaaacatag
    10621 ggagaatgtt taatgcataa aaaatattta ttgctatttt ttctgttatt atcagttgca
    10681 ttgttatata gctgttccaa gcaaaatgag aaagctcaaa ataaatctgt taatccaaat
    10741 gagtatgcaa agcataaatc gaaaatggat gaacattggg aaacatatca cggccaagtt
    10801 tatcatgtgt tttatcaccc attaattaca gacccaaaag ttgcgtttac aggagataca
    10861 caccaagcta agggcaacaa tgattggatg attactgtaa gtgaatttaa aaaatcattg
    10921 aacgaattat ataaaaataa ttacatcatc gttaatccac atgatgtata cgacttatca
    10981 tctcatcatg taaaagccaa aaaaataaaa ttgccgaaag gtaaaaagcc actaatcatc
    11041 tcaattgatg atatgaatta ctatagttac atgagacacc atggttatgc tgaccgttta
    11101 gtactcgata aaaataaaca tgttgtttct gaaactacag acaaaaaagg ccatgctact
    11161 tattctgatg acaatgatat cgtgccaatt ttaaataaat ttgtaaaaga gcatccagat
    11221 ttttctttaa atggtgaaaa aggtgttgtt gctttaaccg gttatgaggg cgtattaggt
    11281 tatcgcacga atgaattaac gagtaaagat tatacaaaga ataaaaaggc agctgaggaa
    11341 gttgttcgcg caatgaaacg ggatggatgg tcatttgcga gtcattcata tggtcacatt
    11401 aattttgaaa aaacatcttt agaaggtatt aaacgtgata ctaaaagatg gaaagatgaa
    11461 gttgaaccaa ttgtaggtaa aacagatatg tttgtatttc cacatggcgc acaggataga
    11521 catacacaag cgtatgatta tttggtagat gaagcagagt ttaagtttat tgcaggtgtc
    11581 ggtccaaaca attttacaga tatatcagca acgaacgttt accaagatag ggttgcgatt
    11641 gatggtctaa acttatttga atttaaatat aaactcaaac cgttttttaa tcctgaaaat
    11701 gtatatagta aacaagatag acgttacttt aaagggaatc gggattatga agaatagtta
    11761 aatttaataa aaagatagaa agaaaataac tttacataat aaggcaagga caatatgtgc
    11821 aagaagtaca acttcaatat aaaaatcttg gttaagcatt tttataggca caaatgtctt
    11881 gcctttttta ttttaaaaaa ttttaaaatc aatacaccga attttctgaa aaaggttgta
    11941 atgtattgct aaaaatgata agttattaat catattacac tttagcgtgg tagagaagta
    12001 aagcaaacaa cgaaaatacc aagtgatttt aaatacagta ttgaaagtaa agggtggtcg
    12061 agtgaataat tcagaacaat taattgcgtt aaaagaatcg gaaacagcat ttttaaaata
    12121 ttttaataaa gcggattatg agttagttga ttttagtgtg gttgaaaagt tggactggaa
    12181 acaattgaac catgaggatt tgcagcagat gggtgagcga aatttttggc aacatgagca
    12241 tcaaatttat gcacttcgaa atgattttac agatcaatta ttgcgttatt actcaatgta
    12301 tccaactgca gcaacaaaag tggcatacac aggtttaatc attcggaata atgaagcggc
    12361 tgtacaagtt ggtttggaaa actatgcacc atcattagcg aatgtgcaac agagcttaaa
    12421 gctctttatt cagtttattc aacagcagtt gagagacaac gtgcattttg ttgtattggg
    12481 acattatcaa ttgcttgatg ccttactcga taaatcgtta caaacaccag acatactatc
    12541 aatgattgag gaacgtaatt tatcgggact ggttacatat ttgtcgactg aacatccaat
    12601 cgtgcaaatc ttgaaagaga atacgcaaca acaattaaat gtattggaac actatattcc
    12661 aaatgaccat ccagcactcg ttgaattaaa aatatgggaa cgttggttac ataaacaagg
    12721 ttacaaagac atccatttag atattactgc gcagccacct agatcctatt acacaggttt
    12781 atttattcaa tgtcattttg ctgaaaatga atctcgagtt ttaacaggtg gttattacaa
    12841 aggaagcatc gaagggtttg gattaggatt aacactttaa gtaagggagt atgcacaatg
    12901 ttaagaatcg ccatagccaa aggacgtcta atggatagtt taattaacta tttagatgta
    12961 attgaatata cgacattatc agaaacatta aaaaatagag aacgccaatt attattaagt
    13021 gtagataata ttgaatgcat tttagtaaaa ggaagtgacg tgccaatcta tgtggaacaa
    13081 ggaatggcag acataggcat tgttggtagc gacatattag atgagcgcca atataatgtt
    13141 aataatttgt tgaatatgcc ttttggagca tgtcattttg cggttgcagc gaaacctgaa
    13201 acgaccaatt atcgtaaaat cgcaacgagt tatgttcata ctgctgaaac atattttaaa
    13261 tcaaaaggta ttgatgtcga attgattaaa ttgaatggct ctgttgaatt ggccggtgtt
    13321 gtagatatgg tagacggaat tgtcgacatc gttcaaacag gtactacgct aaaagcgaac
    13381 ggactggttg aaaagcaaca tattagtgat atcaatgcaa gattaataac taataaagca
    13441 gcttatttta aaaaatcaca attaatagag caatttattc gctctttgga ggtgtctatt
    13501 gccaatgctt aatgcacaac aatttttaaa tcaattttca ttagaagcac cattagatga
    13561 gtcattgtat ccaattattc gcgatatttg tcaggaagtt aaagttcatg gagataaggc
    13621 tttgaaaatg tataatctaa cattcgatca tacgaaaaca gatcatttag aaattagtca
    13681 tgaacaaatt aaagcagcat ttgacacatt agatgaaaaa acaaaacaag cattacaaca
    13741 aagttatgaa agaattaaag catatcaaga aagtattaaa cagacgaatc aacagttaga
    13801 agaatcagtg gagtgttatg aaatatacca tccactagaa agtgtcggta tttatgtgcc
    13861 tggtggtaaa gcaagttatc cttcaacggt attaatgaca gcgacgttag cacaagtagc
    13921 aggtgtggaa aatattgtcg ttgtgacacc acctcaacct aatggtgtgt ctcaagaagt
    13981 attagcagca tgctatatta cgcaagttga tcaagtattt caagttggtg gtgctcaaag
    14041 tattgctgcg ttaacttatg gcacagaaac gatacctaaa gttgataaga ttgtaggtcc
    14101 aggcaatcaa tttgttgcat atgccaagaa atatttattt ggacaagtag gtattgacca
    14161 aatagctgga ccaacagaaa tagcacttat tattgatgaa acagcagatt tagatgccat
    14221 cgtatatgat gtttttgcac aagcagaaca tgatgaatta gcacgtacat atgccattag
    14281 tgaagatgcg caagtcctta aagatttaga atcacgtatt gctaaagcat tgcctaatgt
    14341 ggacagatac gacattgttt ctaaaagtat cgctaatcaa cactacctta tccatgctag
    14401 taattttgat gaagcatgcc atgtcatgaa tacaatcgcg cctgaacatg cgtcgattca
    14461 aacagtaaat cctcaaccat atattgaaaa agtgaaatat gtgggtgcat tgtttattgg
    14521 acattattcg ccagaggtca taggagatta cgttgcaggt ccaagtcatg tattacctac
    14581 aaatagaaca gctagattta ccaatgggtt atcggtcaat gatttcttaa cacggaacac
    14641 ggtcatccat ttatcaaaag atacgtttga acaaattgct gattcagcac aacatattgc
    14701 tcatgttgaa gcattataca atcaccagca gtctatttta atacgtcagt cttaggggag
    14761 tgtaattgaa atgatttata ttgataaaaa tgaaagtcca gttacgccgt tggatgaaaa
    14821 aacaatgacg tctattatta gtgcaacgcc atataattta tatcctgatg cagcatatga
    14881 acaattcaag gaagcttatg ctaagtttta cggattatcg cctgaacaaa ttattgcagg
    14941 aaatggatct gatgaattga ttcaaaagtt aatgctgatc atgccagaag gtccggcatt
    15001 aacgctaaat cctgattttt ttatgtatca agcatatgcg gcacaagtaa atcgtgaaat
    15061 tgcatttgta gatgcaggat cagatttaac gtttgatttg gaaaccattt taacgaaaat
    15121 cgatgaagta caaccatcat tttttattat gagtaatcca cataaccctt caggcaagca
    15181 atttgatacg gcatttttaa cagctattgc agataagatg aaagcattaa acggatactt
    15241 tgtcattgat gaagcatatt tagattatgg tacggcatat gacgtggaac tggcaccaca
    15301 catcttaaga atgcgtacat tatcaaaggc gtttggaatt gccggcttaa gattaggtgt
    15361 cttaattagt actgctggaa cgataaagca tattcaaaaa atagaacatc catatccatt
    15421 aaatgtattt acgctaaata ttgcgactta tatttttaga catagagaag agacaagaca
    15481 atttttaacg atgcaacgac agttagctga gcagttaaaa caaatatttg atacacatgt
    15541 tgcagataaa atgtcagtgt tcccatcaaa tgctaatttt gtacttacta aaggctcagc
    15601 agcgcaacaa ttaggacaat acgtatatga acaaggattt aaacctcgct tttatgatga
    15661 gccggtgatg aagggctatg taagatactc aattgcaaca gcatcacagt taaagcaatt
    15721 agaagaaatt gttaaagaat ggagtgcaaa atatgattta tcaaaaacaa cgaaacacag
    15781 ctgaaacgca actaaatatt tcaatatctg atgatcagtc accatcgcat attaatacag
    15841 gtgtgggctt tttaaatcat atgttaacct tgtttacatt tcatagcggt ctgtcattaa
    15901 acattgaggc acaaggtgat attgacgtag atgatcacca cgtaactgaa gatatcggca
    15961 ttgtcattgg ccaattgtta cttgaaatga ttaaagataa aaagcatttc gttcgttatg
    16021 gaacgatgta cattccaatg gatgaaacat tagcacgtgt cgttgtggat ataagtgggc
    16081 gcccatacct atcattcaat gcatcattaa gtaaagaaaa agttggtacg tttgatacgg
    16141 agttagtaga agaatttttt agagcggtcg taatcaatgc aagattaaca acgcatattg
    16201 atttaattcg tggaggtaat acacaccatg aaattgaagc tatattcaaa gcgttttccc
    16261 gtgcattagg catagcgcta actgcaactg atgatcagcg tgtgccgtca tcgaaaggtg
    16321 tgattgaatg attgtcatcg ttgattatgg attagggaat attagtaatg taaaacgcgc
    16381 tattgaacat ttagggtatg aggtggttgt ctcaaatacc tcaaaaataa tcgatcaagc
    16441 agaaacaatc atattgcccg gtgtcggcca ttttaaagat gcgatgtcag agataaaacg
    16501 attaaatctc aatgcaatat tggctaagaa tactgataag aagatgattg gtatttgttt
    16561 aggcatgcaa ttaatgtatg agcatagtga tgaaggcgat gcatctggat tagggtttat
    16621 cccaggaaat atttcgcgta tccaaacaga atacccagtg ccacacttag gctggaataa
    16681 tttagtgagt aagcacccta tgttaaatca agatgtttat ttcgtacatt cttatcaagc
    16741 gccgatgtca gaaaatgtaa ttgcatatgc gcagtatggg gctgatattc cggcaattgt
    16801 tcaatttaac aattatattg gtattcaatt ccatcctgaa aaaagcggta catatgggtt
    16861 acaaattttg cgtcaggcaa tacaaggggg atttataaat gattgaatta tggccagcga
    16921 ttgatttgat tgggtcaaca agtgtgaggt taacagaggg taaatatgat agtgaagaaa
    16981 aaatgtcacg ctcggctgaa gaaagtattg cttactatag tcaatttgaa tgtgtgaatc
    17041 gtattcatat cgtcgacttg ataggtgcta aggcacagca tgcccgagag tttgattata
    17101 ttaagtcatt gaggagatta acaaccaaag atattgaagt aggtggtggc attcgtacga
    17161 agtcacaaat catggactac tttgccgcag ggattaatta ttgcatagtt ggaacgaaag
    17221 gtattcaaga tactgattgg cttaaagaga tggcacatac atttccaggt cgcatttatt
    17281 tatctgttga tgcctatgga gaagatatta aagtgaacgg atgggaagag gacacagagt
    17341 taaatttatt tagttttgtg agacggttat cggatatacc tcttggcggc attatatata
    17401 ctgatattgc taaagatggc aaaatgtccg gacctaactt tgaattaact ggtcaattag
    17461 taaaggcaac gacgattcct gtcattgctt ccggtggtat tagacatcag caagatattc
    17521 aacgattagc gtcgctaaat gttcacgctg ctattatagg aaaggctgca catcaagcat
    17581 ctttttggga ggggctaaaa tgattaaaaa acgtatcatt ccatgtttag atgtcaaaga
    17641 tggtcgtgtc gttaaaggga ttcaatttaa aggattaagg gatattggga atcctgttga
    17701 tttagcaatg tattacaatg aagcgggtgc tgatgaatta gtatttttag acatctctaa
    17761 gacggaagag ggtcatagct taatgctaga agtgattgaa cagacagcgt cacgcttgtt
    17821 tatccctctt actgtagggg gtgggattca aagtctcgat gatattaccc aattgctaaa
    17881 tcatggtgca gataaagtat cattaaattc aagtgcttta aaaaatccac agctcattaa
    17941 acaagcgagt gataaattcg gtagacaatg catctgcata gcaattgata gctattatga
    18001 tcctgaaaga aaagcacatt attgttgtac gactggtggt aaaaaaatga caaatattaa
    18061 agtatatgac tgggtacagc aagtagaaca gttaggtgca ggtgagctcc tcgttacaag
    18121 tatgggacat gatggtatga aacaaggctt tgatattgaa cacctagcaa atattaagtc
    18181 tcttgtaaat attccaatca ttgcttctgg tggtggtggc aatgcacaac actttgtaga
    18241 attatttgat cagacggatg tttctgcagg tttagctgca agtatattac atgatcgaga
    18301 aacgacggtt caatctatta aagaagtgat acggcaaggg ggtatagcag taagatgacc
    18361 aaatataaaa ttgattttag caaaggttta gtgccagcaa ttttacaaga taatcaaaca
    18421 aaacaagtat tgatgttggg ttatatgaac caagaagctt ttgataaaac gatagaagat
    18481 ggtgtggtat gtttctattc gcggtcgaaa caacggctat ggacaaaagg tgaaacatct
    18541 ggtcatacgc aacgtgttaa agatattcat gtagattgcg acaatgacac tattttaatt
    18601 gatgtcatac caaatggacc aacatgtcat acaggcagtc aaagttgttt caacacagaa
    18661 gttccatttt cagtgcaaac attagcgcag acagttcaag atagtgccca atccaataat
    18721 gaaaagtcat atacaaaata tttattaaca gaaggtatag aaaagattac aaaaaaatac
    18781 ggtgaagaag cttttgaagt cgtaattgaa gcaattaaag gtgacaaaaa agcatttgta
    18841 agtgaagtag cagatgaact ttatcattta tttgtcttga tgcatgcgct tggcgtcgat
    18901 ttttcagaaa ttgaggcgga attagcgcgt agacatcata agcgcaataa ctttaaaggt
    18961 gaacgacaaa atatcgaaca gtggtaaagc aagtatggac taagatataa ggaaaaggat
    19021 catggcttat acacttacaa atattgtgga aaacgtgaca ttttcaagtt taaaatacga
    19081 caccaacata ttttaactat gaatgctgtg atggtactaa agttgcgaac tcgttataga
    19141 taagtagtgg ataatcacaa tacgaaatca aaaataatta taaaaagtaa attgagcaac
    19201 tcaggaatag atgtcactgt taaagatgtc gaaaagtata tgaatcgata taatgaagtt
    19261 atgaagggaa aaaatggcga aaaagctaaa gagttatgtt tgtcgttact acctattaat
    19321 atcatagttg tctttacatt ctttgtattt atactataaa tacaaatata tctagcctga
    19381 aatagaaatg tcatagccta tttaaaagac aatctccatt agaactaaga tatgcatccc
    19441 gaaagttaga ctaaaaaact aactttatgg gatgtatttt tatgctaatc atcataaatt
    19501 cgagattaag ttaaaggtag ttcaagagta attaaacaat aaattaaaaa tagtaggata
    19561 cttactttga gggaagaaaa ttaactgtat atatttagtt taggaacaag tattacggtt
    19621 tatcctgata caattattgt ggatgggatg atatttttag gtttaaaata cgacaccagc
    19681 aaacataata actgtaatag ctcataaatc tccccatata gctaatctaa aaaaataata
    19741 catcattgga attaagcccc aagcatgtaa atattaaaaa tcaaaaaaga tatatgtaaa
    19801 aaagttacaa tttgcataat taaattgtgt ctaattattg actaattaaa ttttgccaaa
    19861 tataatatta attaataatt tgaaatgatt agcgtataca ctttaaattc tctttggaga
    19921 atatattttt taaatacaaa tgtaaacgct ttctcgtcaa attaaacaat agaaaggatg
    19981 gtcattatga gtgcttggtt aagtaaatta tttgagttta ttcctcgaat aattatcaat
    20041 ttgtttatct aaaataaaaa aatagaggtg ctgacaatga tgaaaagtca aaataagtat
    20101 agtattcgta aatttagtgt aggtgcatct tccattttaa tagctacatt actattttta
    20161 agtggtggac aagcacaagc agctgagaag caagtgaata tgggaaattc acaggaggat
    20221 acagttacag cacaatctat tggggatcaa caaactaggg aaaatgctaa ttatcaacgt
    20281 gaaaacggtg ttgacgaaca gcaacatact gaaaatttaa ctaagaactt gcataatgat
    20341 aaaacaatat cagaagaaaa tcatcgtaaa acagatgatt tgaataaaga tcaactaaag
    20401 gatgataaaa aatcatcgct taataataaa aatattcaac gtgatacaac aaaaaataac
    20461 aatgctaatc ctagcgatgt aaatcaaggg ttagaacagg ctattaatga tggtaaacaa
    20521 agtaaagtgg cgtcacagca acagtcaaaa gaggcagata atagtcaaga ttcaaacgct
    20581 aataacaatc taccttcaca aagtcgaata aaggaagcac catcattaaa taagttagat
    20641 caaacaagtc aacgagaaat tgttaatgag acagaaatag agaaagtaca accacaacaa
    20701 aataatcaag cgaatgataa aattactaac tacaatttta acaatgaaca agaagtgaaa
    20761 cctcaaaaag acgaaaaaac actatcagtt tcagatttaa aaaacaatca aaaatcacca
    20821 gtagaaccaa caaaggacaa tgacaagaaa aatggattaa atttattaaa aagtagtgca
    20881 gtagcaacgt taccaaacaa agggacaaag gaacttactg caaaagcgaa agatgatcaa
    20941 acgaataaag ttgccaaaca agggcagtat aaaaatcagg atcctatcgt tttagtgcat
    21001 ggtttcaatg ggtttacaga tgatattaat ccttcagtgt tagctcatta ttggggcggt
    21061 aataaaatga acattcgcca agatttagaa gaaaatggtt acaaagctta tgaagcaagt
    21121 ataagtgctt ttggaagtaa ctatgaccgc gcagttgaac tttattatta tatcaaaggc
    21181 ggtcgtgtag attatggtgc agcacatgca gcaaaatatg gacatgaacg ttatggaaaa
    21241 acatacgaag gaatttacaa agactggaaa ccaggacaga aggtacacct agttggacat
    21301 agtatgggcg gtcaaacgat acgtcaacta gaagaattac tgcgtaatgg taatcgtgaa
    21361 gaaatagagt atcaaaagaa acatggtggc gaaatttctc cactattcaa aggtaatcat
    21421 gacaatatga tttcatcaat tactacttta ggaacaccac ataatggtac acacgcatca
    21481 gatttagctg gtaatgaagc tttagtgaga caaatcgtat ttgatatcgg taaaatgttt
    21541 ggtaataaaa attcaagagt agacttcggg ttggctcaat ggggtctaaa acagaagcca
    21601 aatgaatcat atattgatta tgtcaaacgc gttaaacaat ctaatttatg gaaatcaaaa
    21661 gataatggat tttacgatct gacgcgtgag ggtgcaacag atttaaatcg taaaacgtcg
    21721 ttgaacccta acattgtgta taaaacatac actggtgaag caacgcacaa agcattaaat
    21781 agcgatagac aaaaagcaga cttaaatatg tttttcccat ttgtgattac tggtaactta
    21841 atcggtaaag ctactgaaaa agaatggcga gaaaacgatg gtttagtatc cgttatttct
    21901 tctcaacatc catttaatca agcttataca aaagcgacag ataaaattca aaaaggcatt
    21961 tggcaagtga cgcctacaaa acatgattgg gatcatgttg actttgtagg acaagacagt
    22021 tctgatacag tgcgcacaag agaagaatta caagattttt ggcatcattt agcagacgat
    22081 ttagtgaaaa ctgaaaagct gactgatact aagcaagcat aatttataaa gtaaagggag
    22141 gaattaataa tgactgcaga cttctttcaa ttaatcggat cattatttag aattctaaaa
    22201 gaattattca agtaaaacat tggcgaggcc ccaacataaa gaatttcgaa aagaaattct
    22261 acaaacaatg caagttggcg gggccccaac aaagaagctg gcggaaagtc agcttacaat
    22321 aatgtgcaag ttggcggggc cccaacatag aagctggcgg aaagtcaggt tacaataatg
    22381 tgcaagttgg ggtgggacga cgaaataaat tttgcgaaaa tatcatttct gtcccactcc
    22441 cattggcatt tacgaagttt aaatgtgcaa ttagaatata tgtataacaa tattaaacac
    22501 gcggtaaaac gaagtcagtc aattcaaact gatttcgccc accgcgtgtt tttaacatag
    22561 cttaataatt aataagcatt aatgttcaat ttatatggtt gttttccaat aataaaccta
    22621 aagatataga attcacgcaa tatcatgccg acacctatac ataatcctaa aatgaatagt
    22681 agtgatatcg ctagaaagac cattgtatta tcctcaaata tatttgtata tgcaaacaat
    22741 gagtctagaa tgattggatg taataaataa ataaagaatg agaaagcact aatcatttga
    22801 atcgtattaa ataacattgt tttaaaatgc gtgcaaatac ccaagataac aataaacata
    22861 atactattat atggtgttaa tgaatatgaa aagctggtaa cgttccaata gtctccattt
    22921 gctaacgcaa taaacacaaa ataagtagct acagctaata caatcataat aactaaataa
    22981 cgttctaaga aatttaatac acgttcgtag ttataaccca tatatgcacc taagaagaaa
    23041 taaaaaatcc atccgaatat tatagtattt tcacttaatg gataatagtg tagcacggta
    23101 tcgtgaaacg ctgtgttgtt cgtaaagtaa tataaaaatg attgctgtaa aataaaagat
    23161 aacaataata atattttact gttgaatagg ttatagttaa ttttaaaaat gatataactc
    23221 aaaataaaga attgcatgat aacaacgata aaatagccat accattgacc taataggaca
    23281 ttttcaatga attgtttatt gaaacttgaa tctgttaata atgattcact ataactgtaa
    23341 aacaatccca ttaatatgta aggaataagt atatatttta cgcgtgtagt taagtatcta
    23401 taggtgactt tttggtaatt caaggttgtc agtaactgtg acaagataat aaagcaaggt
    23461 gtaccaaaaa tcacaatatt acgaatgtaa aattgtaaca ctaaggatcc accctccata
    23521 ttttcatgtt ttaaagtaat ttgtgtaagt aaatgtgtga taataataat tgcacatata
    23581 atagcacgta aatatacgag ttcaagtcta atctttttca tggaatccgt cccatctctt
    23641 aattaaatgc tcaaaagcat catcactaat taatattcta gggatgtaat aatcattgga
    23701 gttcggagtg actgcttttt cctctaatga aaaaccgtat tttaacccag cttttttgat
    23761 taccggtaat ttatcgtcat tcatcaagcc ataaggatag gctatagttt tctgcgactt
    23821 tttaaagttt ttagttagat atttttcact tttgtttaaa tcttttatga ttgtagcttc
    23881 agaagctttc attaattttg acttattatt tttagataag ttatgcaaat cgtgggtatg
    23941 tgtttcaaat tcccataacc cagttttata catttctttt agttcttttt tactaatcat
    24001 atcgaggttg tgaaagtttt cttccccaac atgacctgtg ataataaacc cagttgccgg
    24061 tattttatat ttttttaaga ttggataagc attttcataa atagtttcat ccatatcatc
    24121 aaagttaatc catacacttc gttttggaaa cttacctttt ttcttgtaat ataaaaattc
    24181 tttcaaggtt aaaaatttag catcatgtga ttttagccat tttatttgag attcaaattg
    24241 tgattgacta acactataat ttttaatttc tttactacta gaaaagaagt aaataaaatt
    24301 attcagaaaa ttcgcttttc ttacacggtg ataatttaat gccagagcac tattttcttt
    24361 atatttcagt tttttaggtg aatcgtcatc tgcatttgca atatgatgac catccagtgt
    24421 gcttacaggc aatatgatca agatactcaa cactaaaatt ataaattttc tatacttcac
    24481 gattctcttc ctctctgcca tttttgaatc aatatgctaa ttgtaaaaaa tacaaaaatg
    24541 ataatcgcga aaatgcccat agtttcaaat atatctaaaa tttcagtatt ttcaatgttt
    24601 aaagcaacac gtattgtatt gatactttcg tcatgaattt caaatatagt accaatataa
    24661 acgagtagaa caactaaaca atatatccaa aagacacaag atatagcgat aagtgctgtt
    24721 tctcttacaa tatttagcga tgattttagc gttgggtatt ccctctgtct gggcttgacc
    24781 atgttgcgta accacctttc ttacgtttta atgcttttgg aaatgcgaca agaactactg
    24841 ctgcgttaat aatccagtat actgtcggat accaacttac aaatatgagt ccagccatat
    24901 tctttttctc gtagcgacta tcaataaaga gtgcgactgt aaattgaata acgtttataa
    24961 aagtcatagt aaatgatgat agtagaaata ttgaaaaact atatgtcata aatgtatagt
    25021 ctaagaagtt tgctgttatg aacaaatagc ctaaatatag aagcactata tatacccata
    25081 aaatcgagat gatttgctca aacatcaaaa tatataaagg aaaccttttc gttttcattg
    25141 tgctaaaaaa gtctcgtagt aatacttcgt gtcccccttg agcccatctc acgcgttgct
    25201 tccaaagacc tcccaatgtt tctggaacca acatccaaca catggcaagc ggttcatact
    25261 taatacgata tccacgtaaa tgcaatttcc aagaaactgc aatatcttcg gtaatcatat
    25321 cagtatccca gtagccaacg tcgacaactg cacttttttt aaatagagtg aagacacccg
    25381 aaatagtatt gactgcgcca gcaagtgtct gacttcgctt aatacagcca attaaacttg
    25441 catattctat cgtttgaatt ttacctaaaa tagaactctt atttcgaatt ctaggattac
    25501 ctgtaactgc accaagtttt ggatcatgtt tgaaattctc aatcatataa tatggtgcat
    25561 cttgatcaac gatagtatct gcatccaagc acattacata atcatatgaa gcctgtttaa
    25621 tgccttgatt gagtgcgttg gctttacctc tgttttcttg taaatcgacg aaaataaagt
    25681 cattattttc tttgatttta tagatgagtt ctgctgtatt atctgaactt ccatcattaa
    25741 tgataataat ttctttcttc tcgtatttga gtgcaagaac attagacaac gtatcttcaa
    25801 tcgtttcact ttcgttataa caggcaagta aaaatgtaat gccttctaat tcatccacat
    25861 ttatgtcagg cttcttgttc aatgaatatc taatttctct ggtaaaatag aaataaattg
    25921 aaccgacaat ccagtaaata gacataaata caggataaaa aagcaaaaag ttaaaaaatt
    25981 gcaatttctt tacctacctt tcgttagtta ggttgtaagc catatggtaa ttgatagtat
    26041 tttaatttgc aatagattgt tgttataatt aaacggaaat atttgtaatt gcaacttaat
    26101 tttcctgtaa catagtgtga ttaattttca gtagggggtt ataaaaattg aaggataaga
    26161 ttattgataa cgcaataacc ttattttcag agaaggggta tgacggtaca acacttgatg
    26221 atatagctaa aagtgtaaat ataaagaaag cgagtttata ttaccatttt gactcgaaaa
    26281 aaagtattta cgaacaaagt gttaaatgtt gttttgatta ccttaataat attattatga
    26341 tgaatcaaaa taaatcgaac tattcaattg atgctttata tcaattctta tttgagttta
    26401 ttttcgacat cgaagaaagg tatattagaa tgtacgttca attatctaat acgcctgagg
    26461 aattttctgg aaatatttac ggacaaatac aagatttaaa tcaatcatta agtaaagaga
    26521 tagcaaaatt ctatgatgaa tcaaagataa agatgacaaa agaagacttt cagaatttaa
    26581 tattgctgtt tcttgaaagt tggtatttga aagcatcctt ttcgcaaaaa tttggagcag
    26641 tggaagaaag taaaagtcaa ttcaaagatg aagtgtattc gctactaaat atatttttga
    26701 agaaataatt tttgttacta gtttgtaata attaacttac ttttgtaaaa aaagacatga
    26761 gattattttt ttaaatctat ataaagttga caatacaaat cgatattgag aatattaaga
    26821 tgtatatgaa ttttataaat taaatgcaat acattaatat aaatatcaat tgttgcaaaa
    26881 tacgatttgt tcaatgattt gataatatta ttctttatat ttgtgaatgg ttaagtttgt
    26941 ctttgaacat attataaaag tgtaatgttc ccctgaaaag aataagttgt catctaatta
    27001 caggaaatcc gcataaatta gatgaaatgg aaagtaataa gtaataattt attgataagc
    27061 gcctatgtga tggtaaatca tgacataggc gcttttttta taagttaaaa atgtaaataa
    27121 aaattatata aattacccac atctttttaa aaggtgtggg ctttattatc attaacccaa
    27181 ctcacagtga cgggttacgc aaggtattga attaccgagt acgggcacgc tcggtgttgt
    27241 aaagagcaaa taatcaagta atgatgatgc ttctactcga ttataagaaa gccatgatag
    27301 agtacgatgg tatctagttt tattattaat aggtttggat atttaaagtt ggacaatatt
    27361 atatcttgtg caaaaatata aataagttat acataatggt agagaatcat gatataattt
    27421 taaacgataa aatatttata taaataatta gagaaaatgt agttgtgtat gttttgtggt
    27481 cgttaaacta gatataattg tccgatttat aaaacataca taatgaatac aatgattgat
    27541 tatgtggagg aaaccatgaa agaaaagttt gatttagtaa aactattaaa tattctaaag
    27601 aagaatatta aattattgct tattttaccg gcaatatgtc ttgtagtaag tgcggcatta
    27661 accttttttg ttatgcctga caaatatact gcttctactc aaatattagt gaacatgaaa
    27721 aagtcctcaa gtgatttagc tttccaaaat gttcaaagta gtttgcagtc tgttaataca
    27781 tatacggaaa ttatcaaaag tcctagaatt ctagataaag tatctagaga atttgatggt
    27841 cagtattcaa cagctgagtt gaattcattt ttaaaagtaa ccaatcaaac aaactctcaa
    27901 attattactg tatcagttac cactgggaat aaatctgaat ctgacaaaat tgttaataaa
    27961 atatctaaag tttttgctca tgacatgcct aaaattatga gtgttgataa tgttacgata
    28021 ctttcctcag cacatgataa tgctgtaaaa gtatctccaa tagtatctgt caacttagtt
    28081 atcagcatta ttgttggtat tgttttagca atattgatta ttttcttaaa agaattatta
    28141 gataagcgta ttaagacaga agaagatgtt gaatcgcaac taggattacc tattttaggt
    28201 tcaatacaaa aattttaatt tacgaggaat taccatgacg aatacacgaa gaagtacatc
    28261 aagtttaatt gtccatgaac aaccaaagtc acctattagc gagaaatttc gaggcataag
    28321 atcaaatatt atgtttgcaa atcctgacag tgcagttcaa agcattgtaa tcacttcaga
    28381 ggcaccaggc gcaggtaagt ctacaattgc agcaaattta gcagttgcat atgcgcaagc
    28441 aggttataaa acactaatcg tagacgggga tatgcgtaaa cctacgcagc attatatttt
    28501 taatttgccc aacaatgaag gcctatcaag tttattgcta aattggtcaa cttatcaaga
    28561 cagtattatc tcaactgaaa ttcaagattt agacgtcttg acgtctgggc caatcccacc
    28621 gaatccgtca gagttaatta catcaagggc atttgcaaat ttgtatgaca cattattgat
    28681 gaattataac tttgtaatta tcgatacgcc accagtgaac acagttacag atgcgcaatt
    28741 attttcaaag tttaccggca atgttgtcta cgtagttaat tcggaaaata ataatagaga
    28801 tgaagttaaa aaaggaaaag aacttattga agcaacaggt gctaaattat taggtgtagt
    28861 cttaaataga atgcctaaag ataaaagtgc tagttactat gcatattatg ggactgatga
    28921 atcatgattg atattcataa ccatatatta gttgatatag atgatggtcc taaaacaatt
    28981 gaaaagagta ttgcactatt aaagcaagct aaggatgaag gtgtaacaag tattgtagcg
    29041 acacctcatc atttgcatcc gagatatgat aatacatttc aacaagtgct tgtgaaattg
    29101 gcggagttaa gaacgcatcc agaagttcaa gcattggata ttaaattatt tcccgggcaa
    29161 gaaattagaa ttacagattc gattctgaaa ggcttagata atggtagtat tcaaggtatt
    29221 aatcgttcga aatatttatt aattgaattt ccaacgggcg aagtaccaca ttatacaaaa
    29281 caattgtttt ttgaaataca atcgagaggt tacataccaa tcattgcaca tcctgaaaga
    29341 aatagaagta tcgccaaaaa tccggaaata ttatatgaat tggttgcaaa tggggcactg
    29401 agtcaattaa catcgagttc tttagttggt ggatttggta aaaacattca aaagttatca
    29461 ttacaattta tagaatgtaa tttggctcat tttgtagctt cagatgcaca ttcatgtgac
    29521 caaagaccat ttttgatgca agaattattt cacaatcata aattgaagaa atattcgaat
    29581 gatattgaag cattacttcg aaatgccagt tcagtaatca atgataattt tgtttattta
    29641 gacagaccga ctaagccagg aaaagtgaag tcgtttttga aatggtttta actaaatata
    29701 ttttttcgaa ttagactggt atacatgtac cagtcttttt gagtatgctt aaaatgaatt
    29761 ataatcatga ttatttacta attcgtataa tacaacttgt ctaattgatt aaggcgaatg
    29821 gctttcaata aaaagactcg ttttttatag tgaaggtagt ttgaaaataa tgaatataaa
    29881 gatgaaggta ttacttttga tatatagggc taaggaagtg tttttatggt attaagaaaa
    29941 gtaatcatgc aggacttaga tcaaattatt gcacttgaaa atattggttt ttcaccggaa
    30001 gaagctgcaa cacgagaggc attgaaattg agaattgaac aaattcaaga aacttttatt
    30061 gtagctgaaa agaatgatga agtaattgga tacatcaacg gtccagtcat aaaagagcgt
    30121 tacattagcg atgatttatt taaaaatgtc tccacaaata atagtgaagg tggctatatt
    30181 agtgtgttag gtcttgttgt agcaccaaat tatcaaggtc agggtattgc aggtcgctta
    30241 ctaaactatt ttgaaactct tgctaagaat catcatcgac atggtgtaac gttaacatgt
    30301 cgagaatcat taatttcatt ttatgaaaaa tatgggtatc gtaatgaagg tgtatcagaa
    30361 tcatgtcatg gcggtattaa atggtataac cttgttaaaa atataaaaaa ggatgatgag
    30421 gtgtaaatat ggcagttgtt tatgtagcag gaggatgttt atggggtgtt gaagcatttt
    30481 ttgcaacaat acctggaatt atacatacag aagcaggaag agcaaatgga agaagctcta
    30541 aattagacgg tccgtatgat ggttatgctg aatgtgtcaa acttcatttc gatgatcgta
    30601 tgttaacaat tacagacatt atgaattatt tatttgaaat cattgatcct tacagtgtga
    30661 atcaacaagg aaatgatatt ggacaaaagt atcgaacggg tttatatagt tgtgtagatg
    30721 accacttaat tgaagcgcgt cagttcattg aacgacgtaa agacagagat caaattgcag
    30781 tggaagtctt accgctgtct aactacatta aaagtgctga agaacatcag caacatttag
    30841 aaaaatatcc agaagatatg catatgtgtc atatttcaaa agatttgcta aataaataca
    30901 agtgattttc agaggagaat agatgtttaa attaaccaaa atcggagaaa tacctagata
    30961 tacttagaaa agaggtaggt attatgtata cgtttgatgc acaatcatta actgctagag
    31021 aaaactataa attattaatt ggttcaatta ttccacgacc tatagctttt gttacaacat
    31081 taaatcaaga tgcatcagta aatgcagcac catttagctt ttttaatatt gttaataatc
    31141 atccgccaat gattgctatt gcagttcaac gtgcaggagg taagagaaaa gatactgcat
    31201 taaatataga acggactgga gactttgttg tgcatattac agacgaagat aatgttcaag
    31261 atattaatga gactgcagcg ccgttagcat atggtgacag tgagttgagt cgtacagaat
    31321 tatcactttt aacatctaca acgattaaga cgccaggaat taaagatgcg aaaatgagat
    31381 tcgaatgtaa actatcgcaa atgatattgc ttggagatgt attagatggg gctgatttaa
    31441 taataggtga aattgtaact tatcatattg atgacagtat ttatgagggt gattttaaaa
    31501 tcaatccaca cgcattacag gctgtttctc gtttagccgg aaatgattat gctaaattag
    31561 ggggaatatt tacgataaag agaccagaaa aataataaaa cacatgctat agcggtatac
    31621 cgtttatgaa ttgaatcaaa agtacacagg tgctaaagta tgtgcttcta aaatgaagac
    31681 tattttttat tacaagaaaa tgtatctagt aaacttaaag tagcaagacc taataaattt
    31741 aatgcatgtt gtgcaccttt tttaccttgg ccagcttcga aatgtttgta agcagctaca
    31801 cttaaaatgc ctatcgttga tagtgatgca aggcgagaaa tgtttttatt gataaagcta
    31861 gctgagtata aagcagcagt agttgcttct gcaatgccga cgtattttac aagttctttt
    31921 tgcaagccaa aagtatgttc aaacagttca atcataccct tatcttcttg caatttaggt
    31981 ttactggctt ggtatagctc tttcgcaagt tttaaattcg ttgcgtaacg caaaatcata
    32041 tttaattcct cccaatattt gattttttgt gaaagatgat tactttatca tttttacccg
    32101 tttctataaa aatgaatcaa ttatgtaacg tatgtgtagt ttaggaatgt ttgctatgga
    32161 aatataattc tgttcactca aaatgtatga aattaatgtg tagttttgtc gagttgctct
    32221 tttaatttgg ttagattgtt ttttagagaa gcggtactat ttttaagtgc atcaacagat
    32281 ttaccttcgt tttgagacat tgagtttatt acagcacgaa gttctgtttc tagtatgtca
    32341 gcgtcgcctt tagcattaga acttaatact ttaatatctc ttaaaaggct atctgatagt
    32401 agtgcatttt tgccagtctc caatttcgaa ttgtaattcc aaccgctact taactcaata
    32461 gcatctaaat cggttatatg atcatttgtt ctcatcatac tttcctccct cgatggaaat
    32521 tacattagta tccgatttta aacggccatt tccatctacc attgacgggg aaacatcatc
    32581 tttataaaac tgtacaaatt ttgtgctagg atatgattgc ataatgtcat ttatagattt
    32641 ttgaaggttc caagcatagt tttttactgt ttctctattt ttttctttat cataataagc
    32701 aatgaactct gatttcatat ttttttgtat ttgtggatat ttgttaatca aagattgtaa
    32761 ctgttgattg aatgcatcac cttttaagtg cataacaggt ttgaattctt ttttgaactt
    32821 atccagatca tcgatactat ctgttctgaa attcaagtat tgccttgtaa cgccgttaaa
    32881 ttcttgtttt acttgaagtt tttcgctcat cgcataatag ctgtcttttt caatttcact
    32941 ttattattat aaactaatca tgattgaagt aactataatt aattaaattt acaaaagata
    33001 tcataaatat taattgtata aatccttatc tgaatttttc gaatcaagaa cgcaaattat
    33061 aagcaaccac gtcatatata gtttctactt ataaataaat tgtttattta tattagacgc
    33121 ttatgtaaag agggcgtaac cttaaatgta attacttata aggggtgaag atagtatgaa
    33181 ttattcgcca aaacaaggtg taagaagtca tggtttaccg cacgacccat ttaaaagtag
    33241 tacagtacca cgtccaatag ggtggatctc tactgtatcg aaagatggga aagataattt
    33301 agcgccttat agtcagtatc aaaacttaac ttgggatccg cctatggtta tgttcgcagc
    33361 aaatcaatcc gttcttggtg atcacgaacg taaagataca gtgaaaaatg ctgaagagac
    33421 aggttggttt gtgtggaata tggcaacgta tgatttaaga gaagcagtta atttatcatc
    33481 gaaagcattg ccaccagaag aggatgaatt taattttgca gatgtaacaa aggaaaaatg
    33541 tatagaagct ccaggatatc gtgtaaaaga atcgccagtt cattttgaat gtgagtacgt
    33601 tcaaacgatt cgtattccaa ctggagatcc agtatctacc gtagatattg ttataggaag
    33661 agttgcccaa gttcatattg acgataaggt tattcttgat aatggtaaat tggacattaa
    33721 gtcgattaaa cctattgcca gattaggata ttacgattac acagtagtga atgagatatt
    33781 tgaaatgaaa gcacctgcag cttctaaaga agagttagca ggtttagaag gaagaaattt
    33841 cgataatcaa tcagatgaaa agatataagt aatttaatca ttttagtgaa ttaaatgcat
    33901 ccttactaaa cattcaactt agtagcgatg cattttttcg ctttataaat gaaaatgtta
    33961 aagcaatatg tcgtggtatt tatattttta aagaagaatc ttttagaatt acacatatat
    34021 tacactaggt ttattgaaaa aatattggta ttactttaat attaaagatg tattttgcat
    34081 ataatattta tcgtttagtt gtaaattaac tatttataaa taaaaggtgg atggtataga
    34141 tatattaata caataattat tttaaaaacg aaacaattat aatgattaaa cataaagcca
    34201 attagccaaa tttatataaa aaaagattat acgcataaac gagaaaaagt aattttaaaa
    34261 atatgaaaac gattacattt agacgaataa aaatgaattt ttacaaattt taacaaaatg
    34321 agtatttttg tatcaaaaaa gagttgtatt aaattgaata gtggcatata ttataatcta
    34381 atgaatgtaa agataataca aggagttatt acatgagtaa aagacagaaa gcatttcatg
    34441 acagcttagc aaacgaaaaa acaagagtaa gactttataa atctggaaaa aattgggtaa
    34501 aatccggaat taaagaaata gaaatgttca aaattatggg gctaccattt attagtcata
    34561 gtttagtgag tcaagataat caaagcatta gtaaaaaaat gacgggatac ggactgaaaa
    34621 ctacggcggt tattggtggt gcattcacgg taaatatgtt gcatgaccag caagcttttg
    34681 cggcttctga tgcaccatta acttctgaat taaacacaca aagtgaaaca gtaggtaatc
    34741 aaaactcaac gacaatcgaa gcatcaacat caacagccga ttccacaagt gtaacgaaaa
    34801 atagtagttc ggtacaaaca tcaaatagtg acacagtctc aagtgaaaag tctgaaaagg
    34861 tcacttcgac aactaatagt acaagcaatc aacaagagaa attgacatct acatcagaat
    34921 caacatcctc aaagaatact acatcaagtt ctgatactaa atctgtagct tcaacttcaa
    34981 gtacagaaca accaattaat acatcaacaa atcaaagtac tgcatcaaat aacacttcac
    35041 aaagcacaac gccatcttcg gtcaacttaa acaaaactag cacaacgtca actagcaccg
    35101 caccagtaaa acttcgaact ttcagtcgct tagctatgtc aacatttgcg tcagcagcga
    35161 cgacaaccgc agtaactgct aatacaatta cagttaataa agataactta aaacaatata
    35221 tgacaacgtc aggtaatgct acctatgatc aaagtaccgg tattgtgacg ttaacacagg
    35281 atgcatacag ccaaaaaggt gctattacat taggaacacg tattgactct aataagagtt
    35341 ttcatttttc tggaaaagta aatttaggta acaaatatga agggcatgga aatggtggag
    35401 atggtatcgg ttttgccttt tcaccaggtg tattaggtga aacagggtta aacggtgccg
    35461 cagtaggtat tggtggctta agtaacgcat ttggcttcaa attggatacg tatcacaata
    35521 catctaaacc aaattcagct gcaaaggcga atgctgaccc atctaatgta gctggtggag
    35581 gtgcgtttgg tgcatttgta acaacagata gttatggtgt tgcgacaacg tatacatcaa
    35641 gttcaacagc tgataatgct gcgaagttaa atgttcaacc tacaaataac acgttccaag
    35701 attttgatat taactataat ggtgatacaa aggttatgac tgtcaaatat gcaggtcaaa
    35761 catggacacg taatatttca gattggattg cgaaaagtgg tacgaccaac ttttcattat
    35821 caatgacagc ctcaacaggt ggcgcgacaa atttacaaca agtacaattt ggaacattcg
    35881 aatatacaga gtctgctgtt acacaagtga gatacgttga tgtaacaaca ggtaaagata
    35941 ttattccacc aaaaacatat tcaggaaatg ttgatcaagt cgtgacaatc gataatcagc
    36001 aatctgcatt gactgctaaa ggatataact acacgtccgt cgatagttca tatgcgtcaa
    36061 cttataatga tacaaataaa actgtaaaaa tgacgaatgc tggacaatca gtgacatatt
    36121 attttactga tgtaaaagca ccaactgtaa ctgtaggcaa tcaaaccata gaagtgggta
    36181 aaacaatgaa tcctattgta ttgactacaa cggataatgg tactgggact gtgacaaata
    36241 cagttacagg attaccaagc ggattaagtt acgatagtgc aacgaattca atcattggga
    36301 caccaacaaa aattggtcaa tcaacagtga cagttgtgtc tactgaccaa gcaaataaca
    36361 aatcgacgac aacttttaca ataaatgttg tggatacgac agcaccaaca gtgacaccaa
    36421 taggagatca atcatcagaa gtgtattcac caatatcccc gattaaaatt gctacgcaag
    36481 ataacagtgg aaatgcggtg acgaatacag tgactggatt gccatccgga ctaacatttg
    36541 atagtacaaa taatactatt agtggtacac caacaaacat tggtacaagt actatatcaa
    36601 tcgtttctac agatgcgagc ggtaacaaaa cgacgacaac ttttaaatat gaagtaacaa
    36661 gaaatagcat gagtgattcc gtatcaacat caggaagtac acaacaatct caaagtgtgt
    36721 caacaagtaa agctgactca caaagtgcat caacgagtac atcaggatcg attgtggtat
    36781 ctacatcagc tagtacctcg aaatcgacaa gtgtaagcct atctgattct gtgagtgcat
    36841 ctaagtcatt aagcacatct gaaagtaata gtgtatcaag ctcaacaagc acaagtttag
    36901 tgaattcaca aagtgtatca tcaagcatgt cggattcagc tagtaaatca acatcattaa
    36961 gcgattctat ttcaaactct agcagtactg aaaaatccga aagtctatca acaagtacat
    37021 ctgattcatt gcgtacatca acatcactca gtgactcatt aagtatgagt acatcaggaa
    37081 gcttgtctaa gtcacaaagc ttatcaacga gtatatcagg gtcgtctagt acatcagcat
    37141 cattaagtga cagtacatcg aatgcaatta gtacatcaac atcattgagc gagtcagcta
    37201 gcacctcgga ctctatcagt atttcaaata gcatagccaa ctctcaaagt gcgtcaacaa
    37261 gcaaatcaga ttcacaaagt acatcaatat cattaagtac aagtgattca aaatcgatga
    37321 gtacatcaga atcattgagc gattcgacga gcacaagtgg ttctgtttct ggatcactaa
    37381 gcatagcagc atcacaaagt gtctcaacaa gtacatcaga ctcgatgagt acttcagaga
    37441 tagtaagtga ctctatcagt acaagtgggt cattatctgc atcagacagt aaatcaatgt
    37501 ccgtaagtag ttcaatgagc acgtctcagt caggtagtac atcagaatca ttaagtgatt
    37561 cacaaagtac atctgattct gatagtaagt cattatcaca aagtactagt caatcaggtt
    37621 caacaagtac atcaacgtcg acaagtgctt cagtacgtac ttcggaatca caaagtacgt
    37681 ctggttcaat gagtgcaagt caatccgatt caatgagcat atcaacgtcg tttagtgatt
    37741 caacgagtga tagcaaatca gcatcaactg catcaagtga atcaatatca caaagtgctt
    37801 ttacgagcac atctggttcg gtaagtactt cgacatcgtt aagtacaagt aattcagaac
    37861 gtacatcaac atctatgagt gattccacaa gcttaagtac atcagagtct gattcaataa
    37921 gtgaatcaac gtcaacgagc gactctataa gtgaagcaat atctgcttca gagagcacgt
    37981 ttatatcatt aagtgaatca aatagtacta gcgattcaga atcacaaagt gcatctgcct
    38041 ttttaagtga atcattaagt gaaagtacgt ctgaatcaac atcagagtca gtgagtagtt
    38101 cgacaagtga gagtacgtca ttatcagaca gtacatcaga atctggtagc acatcaacat
    38161 cattaagtaa ttcaacaagt ggtagtacgt ccatttcaac atcgacaagt atcagtgaat
    38221 caacgtcaac gtttaagagc gagagtgttt caacatcact gagtatgtca acgagtacaa
    38281 gtttgtctga ctctacaagt ttgtcaacat cattaagtga ttccacaagt gatagtaagt
    38341 ctgattcatt aagtacatca atgtcgacaa gtgattcaat cagtacaagt aaatctgatt
    38401 ccattagtac atccacatca ttaagtggtt ctacaagtga aagtgaatcc gactcaacat
    38461 catcaagtga aagtaaatcc gattcaacat caatgagcat aagtatgtct caatcaacat
    38521 caggaagtac aagtacgtca acgagtacaa gtttgtctga ctcaacgagt acatcattgt
    38581 cactaagtgc ctcaatgaat caaagcggag tagactcaaa ctcagcaagc caaagtgcct
    38641 caaactcaac aagtacaagc acgagcgaat ccgattcaca aagcacatca tcatatacaa
    38701 gtcagtcaac aagccaaagt gaatccacat cgacatcaac gtcactaagc gattcaacaa
    38761 gtatatctaa aagtacgagt caatcaggtt cggtaagcac atcagcgtca ttaagtggtt
    38821 cagagagtga atctgattca caaagtatct caacaagtgc aagtgagtca acatcagaaa
    38881 gtgcgtcaac atcactcagt gactcaacaa gtacaagtaa ctcaggatca gcaagtacgt
    38941 caacatcgct cagtaactca gcaagcgcaa gtgaatccga tttgtcgtca acatctttaa
    39001 gtgattcaac atctgcgtca atgcaaagca gtgaatccga ttcacaaagc acatcagcat
    39061 cattaagtga ttcgctaagt acatcaactt caaaccgcat gtcgaccatt gcaagtttat
    39121 ctacatcggt aagtacatca gagtctggct caacatcaga aagtacaagt gaatccgatt
    39181 caacatcaac atcattaagc gattcacaaa gcacatcaag aagtacaagt gcatcaggat
    39241 cagcaagtac atcaacatca acaagtgact ctcgtagtac atcagcttca actagtactt
    39301 cgatgcgtac aagtactagt gattcacaaa gtatgtcgct ttcgacaagt acatcaacaa
    39361 gtatgagtga ttcaacgtca ttatctgata gtgttagtga ttcaacatca gactcaacaa
    39421 gtgcgagtac atctggttcg atgagtgtgt ctatatcgtt aagtgattcg acaagtacat
    39481 caacatcggc tagtgaagta atgagcgcaa gcatatctga ttcacaaagt atgtcagaat
    39541 ctgtaaatga ttcagaaagt gtaagtgaat ctaattctga aagtgactct aaatcgatga
    39601 gtggctcaac aagtgtcagt gattctggct cattgagcgt ctcaacgtca ttaagaaaat
    39661 cagaaagtgt aagcgagtca agttcattga gttgctcaca atcgatgagc gattcagtaa
    39721 gcacaagcga ttcgtcatca ttaagtgtat cgacgtcact aagaagttca gaaagcgtga
    39781 gtgaatctga ttcattaagt gattcaaaat caacaagtgg ttcgacttca acaagtacat
    39841 ctggttcatt gagtacctca acatcattaa gtggttcaga aagcgtaagc gagtctacct
    39901 cgctaagtga ttcaatatca atgagtgatt ctactagtac aagtgactcc gactcattaa
    39961 gtggatcaat atctttaagt ggttccacaa gtcttagcac ttcggattca ttaagtgatt
    40021 caaaatcatt gagtagctcg caaagtatga gtggatcaga atcaacgtca acaagtgtga
    40081 gcgattcgca gtcaagctca acaagtaata gtcaatttga ctctatgagc atcagtgcat
    40141 cagaaagcga ctcaatgtct acaagtgatt cgtctagcat cagtggatca aattcaacga
    40201 gtacatcact ttcaacatct gactcaatga gcggaagcgt atcagtttca acatcgacaa
    40261 gtttaagtga ctcaatatca ggttcaacaa gtgtaagtga ctcgagctca acaagcacat
    40321 ctacatcatt aagtgattca atgtcacaaa gccagtcaac aagtacaagt gcatctggtt
    40381 ccttaagtac atcgatatca acatcaatgt caatgagtgc tagtacatcg tcatcacaaa
    40441 gcacatcggt gtcgacatca ttatcaacat cagacagtat cagtgattct acttcaataa
    40501 gtatcagtgg ttcacaaagt acagtagaat cagaatctac aagtgattca acttctatca
    40561 gtgactcaga atcattgagt acatcagatt cagactcgac atcgacaagt acatcggact
    40621 caacaagtgg ttcaacttca acaagcatat ctgaatcatt aagtacgtct ggttcaggtt
    40681 caacgagcgt atctgactca acatcaatga gtgaatctaa ttcatcgagt gtttcaatgt
    40741 cacaagacaa atccgactca acatcaatta gtgactcaga atcagtgtca acaagcacat
    40801 caacgtcatt gagcacatcc gattcgacaa gcacatccga atcactgagt acatctatgt
    40861 ctggttcaca aagcatttct gactcaacat caacaagtat gtccggctca acaagtacat
    40921 ccgaatctaa ctcaatgcat ccgtcagact caatgagtat gcatcatact cacagcacga
    40981 gcacatctcg cttatcaagt gaagcaacaa cgagcacgag tgaatctcag tctacattaa
    41041 gtgcaacatc tgaagtgact aaacataatg gcacaccagc acaaagtgaa aaaagattgc
    41101 cagatacagg tgactcaata aaacaaaatg gattactagg tggcgttatg acattattag
    41161 ttggtttagg tttaatgaag agaaagaaaa agaaagatga aaatgatcaa gatgattctc
    41221 aagcataaat tgaatttata gctggctaaa attaaaaaga ctgaggcacc aatcactaga
    41281 aaaatcgata taggatttta ataattagtg atgaaccctt agtcttttta atgtttttga
    41341 tataaaagag gtgcaacatg ttaaaacttt tacaacaata cgaatataaa attatatata
    41401 aacgtatgct atacacatgt tttatcttgt ttatttatat attaggcaca aacatttcca
    41461 tcgtttccta taacgatatg caagtaaaac atgagtcatt ttttaaaata gctatttcta
    41521 atatgggtgg agacgttaat actttaaaca ttttcacttt aggattaggt ccgtggttaa
    41581 catcaatgat tatcttgatg ctgatttctt atcggaatat ggataaatac atgaaacaaa
    41641 caagtcttga gaagcattat aaagagcgca tcttaacatt aattttaagt gttattcaaa
    41701 gttattttgt gattcatgaa tatgtttcga aagaacgtgt tcatcaagac aatatttact
    41761 taacaatatt gattttggtt actggaacga tgttattagt ttggttagct gacaaaaata
    41821 gtcgctacgg catcgcagga ccaatgccga ttgtaatggt tagtattatt aaatcaatga
    41881 tgcatcaaaa aatggaatat atagatgcaa gtcatattgt gattgcgttg ttaatcatcc
    41941 ttgtgataat cacattgttt atcttattat ttattgaatt agtagaagtg agaataccat
    42001 atatcgattt aatgaacgtt tccgcaacaa atatgaaatc ttatttatct tggaaagtta
    42061 accctgcagg cagtattact ttaatgatga gtatttcagc ttttgttttc ttaaaaagtg
    42121 gcattcattt tattttatct atgtttaata aaagcatatc agatgacatg ccaatgctga
    42181 catttgatag tccagtaggt atttcagtat atttagtgat tcaaatgtta ttgggttatt
    42241 ttttatcgag atttttaatc aatactaaac aaaaatccaa agatttctta aagagtggca
    42301 attatttttc aggagttaaa cctggtaagg atacagaacg ttatttaaat tatcaagcac
    42361 gacgcgtatg ttggtttgga ttggcattag ttacagtcat tattggtata ccgctttatt
    42421 tcacattgtt tgtaccgcat ttatctactg aaatttattt ctcagtacaa ctgattgtat
    42481 tagtttacat cagtattaat attgcagaaa caattcgtac atatttatat tttgataaat
    42541 ataagccatt tttaaaccag tattggtaag gaggtaatta tgaaatactt tattccagct
    42601 tggtacgatg accaacgatg gtggcaagac acgactgtgc cgtattatca actacaaaat
    42661 aagacggaat ttgacgatat gattagttta atgggaatgc accttgaaaa tgacttagat
    42721 tatcaactga ttgttctcaa tcatgcacca aatttaagaa catttttaca tcgatatgac
    42781 ttatatgaga caaagtattc gtctgtgttt gatgaaattc aaggattcag tcaccatgcg
    42841 ccacaagcga ttaattatca tcacttaaaa tggccggatg atgttgagtt tgtgtacaca
    42901 ccgtatttat taaaatgtgt gacgagtgaa cagacctata caaatattta ttttagtcaa
    42961 gaagggtatt caatttggtt tgaagaattt gaaagagatc agttacaacg gcgttatatt
    43021 tttgatgaca gaggttattt atcagcgata cgttattttg atgatcaggg agaggcttct
    43081 taccaagaat atttaacgat taatggagat tgtgtacttc atgaagattt gaaaaatggc
    43141 agagtcactg tttcaaaaag atatcaacat cactatcaac aaacagaata taacaatatg
    43201 gctcaactaa ttgaagaaaa atttcaagca atgattgcac aacaaataca tgaagatgat
    43261 catgtgattg tggcttcaga tgctaggcac aatcgacaaa tagccaatca tattccagcg
    43321 aaatcattaa gttattcatt ttttaaaaat agaaatgaaa ctgtgtcaga tgaggaatat
    43381 caatctatcg taaagaatgc ccatttaatt gttgatagtg tgcaactaga acgtgattta
    43441 attagtcatc aagagaagta tcagcgggag aatacaatga ttcgaatcac accatttgaa
    43501 acgagacaat cacctaatat aagtagtcaa ttgatggaaa catttatagg tgtatggata
    43561 gatggtatga gtgacgctga tttgcaacaa atgatgcaac gacttgtgga ttatatcgca
    43621 caggaagatt attaccgttt aattttatta tcgcgccatc aaaatgacat accgatgtgg
    43681 cttcgtgaat gtattacgtc ggtaaatgag gaataccaag ctaaacagaa tgcggatgtt
    43741 aatgtttcag cattaatgac acctgaagat caagatgaca tcattgctgt taagacgata
    43801 catgctgaac atgatgttgt agaagcattg cggacgttgc gacttgtgat agatatgtca
    43861 aaagaacctg acttgtattt acaaattagt gcaattagcg ctgggattcc acaaattaat
    43921 ggtcaacaaa cagattacgt ctctgattat gacaatggcc gtattataaa tacagttgat
    43981 gaattagatg atgcgttaaa ttattattta ttttatttga aaaattggaa ttatgcgtac
    44041 gcctattctt taaaattaat agatgcatat gcttctaaga atattattaa tcagctcgat
    44101 gagttaatag aaggtgaaaa tgatgccacg taaatttaga gttttgcaaa ttggaggaga
    44161 cgatttagaa cctatttttc aacacaaaaa aggtgtgagt tgggattact tcgatattgg
    44221 attgtttgaa tttgatagtg gttatgtaga ggctattgaa gcgattgttg aagcagaagg
    44281 gcgctttgat tttatctata ttcaagcacc atactcggag acattaacga atttattaca
    44341 aatgataagc gaaccataca atacgtatgt tgatgaatca ttttggtcag ttgaatatga
    44401 acaagacgaa aatgtccaaa aatacgttgt tcaaccatta cattaccgga atattgaaga
    44461 acgtaataat aaattagagg cagttagctt ctcaggacag tatggagata aggtttctcc
    44521 taagttagca cttgtgcatc cgaattttaa aggagatgtc gtttaccaag gtaattcaga
    44581 gctcacattg agtggagaat ttgggaaaga atttaaacct atcgcatctt ggcaaaataa
    44641 tctcgtttac gataaagata aagtcattca aatatggcca gaatttgata ttgatggtgc
    44701 ggtcgagttg caatatacat ttagattgat tcagactggc gctgatggtg cattaattga
    44761 acaaatcgta ttgactgatg atatgttaga cagtccttta gagatacctg cgaaaccatt
    44821 tgatgcttat ataagtgtaa ctgttaaggc gcgtgggaac gggacggtac atttaggacc
    44881 tatacacaaa cgatggtcta gattagacat gggtcaattt ttacttggtg gtagtcgttt
    44941 cgtagatagt caacgacagg aatttattta ttatttccac cctggtgata tgaaaccgcc
    45001 actaaacgta tattttagtg gttatcgaac agcggaaggt ttcgaaggat attatatgat
    45061 gaaacgtatg aatgcaccgt ttttacttat cggcgatcct cgtgttgaag gtggtagttt
    45121 ttatatcggt tcatctgaat atgaacaagg tattatcaat gttattgacg agacattaga
    45181 aaagctgaat tttaaatcac atgaattaat attatctggt ttatctatgg gttcatttgg
    45241 tgccttgtat tatggtgctc agttaaatcc tcaggcaatt attgttggta aaccacttgt
    45301 taatattgga acgattgctg aacatatgcg attacttaga ccagaagagt tcggtacagc
    45361 attagacgtc ctggtgtcta atgaaggtga tacatctcaa gcgtcgatac aggcattaaa
    45421 tcaaaaattt tggcaaacat ttcaaaagaa atcattgtca caaactgttt ttgccatagc
    45481 atatatgcaa catgatgatt atgatccaca tgcatttcaa gagctattac ctgtattgac
    45541 agcacaccaa gcaagagtga tgaatcgcag tattcctggg cgacataatg atgattcacc
    45601 taccattgca agttggtttg ttaattttta caacattatt ttggaagaca agtttgggag
    45661 agttcaacat gctgaaaaac aaaacattta aagttacttg gaattatatt tcacaaacta
    45721 catttatgta tggctctaaa gtatcatttt caaatggtga agtgactttt ataaatcctt
    45781 tgatgccctc tggattaccg attcatgagt ggttgatgtt aaagcaattt tcaaaatata
    45841 aaagtgcgcc aagtttacca attttgcgta gaggacagca ttataaattg cattttgatt
    45901 ttgatgcaac gcctgctggt tcagtgtatt ttatcatcat tttttataat aaaaatggca
    45961 caaaactaag tactgagatt gtaaagtcaa atagtattac gattcaatat cctgatgaag
    46021 cctatgctta taaaataaaa atgatgaatg cagcatcgac gagtcttata tttagatgtt
    46081 taacaatcac agagatgacg catcaatatg atttagagta taaaagcatg cgggtgacga
    46141 agataggcga tgaccaatat ggaaatgata tgatcaatgt cattattgca gaaccatctg
    46201 acacctatcc aactatttcg aatgattttt tgaaactgtt tggtcatgtt tggctcgttg
    46261 aacgttggat ggacgacaat atcgagggaa atattaagca actaaagaat gatttgcaaa
    46321 gtcaagatac cctaaaggct attaatttga ttagttatgg gtcaaagtca aatgtttttg
    46381 caacttatgt cgctcagcat ttgggttgca aagtgtatcg tacaagccat gaagatgatg
    46441 atttaaaagg atggcttaca gaacatgtcc caggaaatca tgaactaaaa gatacaaatg
    46501 tcgaggttta ttttaaagaa gagcaagaca aacatctaaa ttatatgtca cgtttaatga
    46561 atccagtacg atttttagat tacttagatg tatcgaaact gaatggtggg gaagtaaatg
    46621 aaacataaac tagatgttac gataaacgaa ttaagactaa agtcgattag aaaaatagtg
    46681 aaacgcatca atacatggag cgatgaagtt aaatcatatt ctgatgatgc gttaaaacaa
    46741 aagacaatag aatttaaaga acgtttagca tcaggagttg atacactaga tacattgtta
    46801 cctgaagctt atgcagtggc acgtgaagcg agctggagag tattaggtat gtatcctaaa
    46861 gaagttcagt taatcggagc gattgtattg catgaaggta acattgcgga gatgcaaaca
    46921 ggcgaaggta aaacattaac ggcaacgatg cctttatatt taaatgctct ttcgggaaaa
    46981 ggaacatatt taattacaac gaatgattat ttagcgaagc gagattttga agagatgcaa
    47041 ccattatatg aatggttagg tttaactgct tcattaggtt ttgttgatat tgtggattat
    47101 gagtatcaaa aaggggaaaa acgtaatata tatgaacatg atattatata tacaactaat
    47161 ggacgtctcg gttttgatta tttaattgat aatttggctg atagtgcgga agggaaattt
    47221 ttaccacaat taaattacgg tattattgat gaagtggatt caatcatttt agatgctgct
    47281 caaacaccat tagttatttc gggtgcacca agattacaat caaatctatt tcatattgtg
    47341 aaagagtttg tagatacatt gattgaagac gtgcatttta aaatgaagaa gaccaaaaaa
    47401 gaaatatggc tgttaaatca aggtattgaa gcggcacaat catactttaa tgttgaagat
    47461 ttatatagcg aacaagcgat ggtcctagtg cgtaatatta atttagcact gcgcgcacaa
    47521 tatttgtttg aatctaatgt cgattacttt gtatataatg gtgatattgt tttaattgac
    47581 cgtattacag gtcgtatgtt accgggaact aagttgcaag ctggacttca ccaagctatt
    47641 gaagcgaaag aaggtatgga ggtttcaaca gataaaagtg ttatggcaac aattaccttc
    47701 cagaatttat ttaaactttt tgaatcattt tcaggtatga cagctacagg aaaattaggc
    47761 gaatcagagt tctttgattt gtattcaaaa atagtcgtac aagtaccaac tgataaagcg
    47821 attcaacgta tcgatgaacc agataaagtg tttcgttcag ttgatgagaa aaacatcgcg
    47881 atgattcatg atatagttga acttcatgaa acggggcgac cggttttact cataacgaga
    47941 actgctgaag cggctgaata cttttcgaaa gtattattcc aaatggatat tcctaataat
    48001 ttactcattg cgcaaaatgt tgcaaaagaa gcgcagatga tagctgaagc agggcaaatt
    48061 ggttccatga ctgttgcgac tagtatggca ggtcgaggca cagatattaa acttggtgaa
    48121 ggtgtcgaag cattaggtgg attagctgtt attattcatg aacatatgga aaatagccgt
    48181 gtagacaggc aattacgtgg tcgttctggt agacaagggg atccgggatc atcttgtata
    48241 tatatttcac tagatgatta tttagttaag cgatggagcg atagtaattt agcggaaaat
    48301 aatcaattat attcattaga tgcacaacga ttatcgcaaa gtaatttgtt taatcgcaaa
    48361 gttaagcaaa ttgtagttaa agcgcagcgt atctcggaag aacaaggggt taaagctcgt
    48421 gaaatggcta atgaatttga aaaaagcatt agtatacagc gagatcttgt atacgaggaa
    48481 cgcaatcgag ttttagaaat agatgatgct gagaatcaag attttaaagc gttagctaaa
    48541 gatgtatttg aaatgtttgt aaatgaggaa aaggtgctaa caaaatcgcg tgttgtcgag
    48601 tatatttatc aaaatttaag tttccaattt aataaagatg tggcttgtgt taattttaaa
    48661 gataagcaag cagtagtgac atttttatta gagcaatttg aaaagcaatt agctttgaat
    48721 cgtaaaaaca tgcaaagtgc atattattat aatattttcg ttcaaaaagt ctttttgaaa
    48781 gcaattgatt catgttggtt agaacaagtc gactatttac aacaattaaa agcaagcgtt
    48841 aatcaacgac aaaatgggca acgtaacgct atttttgaat accatagagt tgccttagat
    48901 tcatttgaag ttatgacacg aaatatcaaa aaaagaatgg tgaaaaatat ttgtcaaagc
    48961 atgattacat ttgataaaga gggtatgcca gttatacatt ttccataaat gaaaaggggt
    49021 agcgcatgac aatatataat ataaactttg gtataggctg ggcgagtagc ggtgttgaat
    49081 atgcacaagc ttaccgtgct aaattattga gacgtacaaa tcaagacact aaatttgtgt
    49141 ttttagattt tatacagtca gagaacatac agacattaac gcaaaacatt gggtttaaag
    49201 atcaagaagt tatttggttg tatcagtatt ttagtgatat ttctttagca ccaacaacgt
    49261 acacgttaga tgacattgaa cgtgagcttg gtcatgacat tagttttaga gaaagaaatg
    49321 gtaagatagt ccgcctatat tttaatggga aatcatcatt tgtaacatgt tatctgcaaa
    49381 atgaacaaaa agatattgtg gatcgtgcag aatttgtaat taatagtatg ttagtacgta
    49441 aagattttta tagttatacg cgcatatttt ctgagtatta tgcaccagct gataataaag
    49501 cgaaattata tatgcgacaa ttttataatc aagatggttc catcgcttat aacgaatata
    49561 ttgatggaga cagctctata tatgtgtttg aagatgcggt gttgtataac aaaacggaat
    49621 ttattgcata ctttttacaa cgattgaatt taacccgaga cgatattgtc atattagacc
    49681 gcgcttctga tattggtcag gctgttttgc agcataaagg tgatagtaaa gttggtgttg
    49741 tgattcatgc tgatcattat agtaataata tgatgagtga gcagcatata ctatggaaca
    49801 attattatga atatcaattt tcaaaagcta aatacattga tttctttatt acagcaacag
    49861 atattcaaaa tcatatggtt tgtcgacaat ttgagcaata tcaaggttat cgcccacgtg
    49921 tttatactat tcctgtggga agcattgatg cattatcata tccaacatta tcaagaaagc
    49981 catatgcgat gattagtgca tctcgtttag cgaatgagaa acatattgat tggcttgtca
    50041 aagcggttat cgtggctaaa cgacaagtgc cagaattaac atttgatatt tatggagaag
    50101 gtagcgagaa gacgcgactg cgtaaaatta ttgatacaca ccgcgcacaa gattatattc
    50161 gtttactagg gcatgttaaa cttgatgaaa tttataatga ttatgagtta tttctttctg
    50221 catcaactag cgaaggtttt gggttaacac taatggaagc ggtaggctca ggactaggta
    50281 tgattggatt aaatgtgaat tatggtaacc caacctttat tcgagatggg gaaaatggct
    50341 acttggtacc ttttgatacc gacgaagata gtgtggatga tgttatcgca aaactagctc
    50401 atgccattgt aatgtatttt aacaatggtc cccagacacc acatgacatc tcttatgaag
    50461 tggcacagca atttatgact caagatatta ttttaaaatg ggaaacatta gttcaagagg
    50521 tgctacatga ttaaattatt tgattacttt aacgatcatt cacgaaagtt atatgagtct
    50581 tttaaagcca gtaagttgga agaagattta acaattgtac tcaatgacaa tggcttttta
    50641 ccggacgatg ttatatcacc gtatcaattt tttgcagata atcataatac agaaaatatg
    50701 aaaccacgat tttttaatca ggttactgta ccagcttttt gggaaattaa aggtaacaac
    50761 aattcggcta caattaatga tatgggacgt ctacgaggta aaatatttta tcaaagtgga
    50821 gagcgcccta gaattgttag tcgtgtcgaa tggtttgatg accaacaacg tgtccgattt
    50881 gtcgattatt attctaagaa tggtattaaa tttgcacaaa ctgtttacga tttaaatcgt
    50941 aaagcgatct tgaagaaata tatgacagca gaaggtaaag aagtgattta tgaaaatttt
    51001 gtaacatctg atgtcatatt ggattggcaa gggaagtctt actttttccc ttcaaagctc
    51061 gcattcgcat tattttttat caagcaactt gaaattacgg aacatcattt cgttattaac
    51121 tcactagccc taccattttc agtgttatat aatttgccat caaaaggtag tgatgttttg
    51181 gtatggcaag aacactgtaa tggtaatgtc ccgggaaata tgcaattgat gtgtaaaggt
    51241 gatatgaaac gacattgtaa tattattatt ccagataaaa acgaatatga aacgatgctc
    51301 aatatagcag acgcaaaggt tcaatctcgt attttacaag gcggatatct atataactat
    51361 cgtagtcgta atagatatac taaagaaatt gtaatattaa ctaactcgga tcaacttcgt
    51421 aacatcaagg tattggttga aactttacca gattttaagt ttcacatcgc tgctattaca
    51481 gagatgtctg ataagctaat gcaacttgat caatatgcta atgtgcattt atacccatct
    51541 attaatattg acagggtaaa tgaactatac cagttatgtg atatttatct tgatatcaat
    51601 gagggtaatg aaattttaaa tgcggtggaa caagcgtttg actatgagtt gttgatttta
    51661 ggttatcgtc aaaccgcaca ccatgctaaa gtaacattat cagagcactt atttgagcat
    51721 aatgatgaga taacaatgga aagtaaagac caattaatac aaatgttaga aagtttgaaa
    51781 gatcaacaac agtttcgaga tgctttatta gcacaaaaag cacatgcaca cgaaatctcg
    51841 cgtgaacaat tcgagcaagt ttttaaacag gcgctcgaaa gctaaaagga aaaagcagtc
    51901 taaaataaaa catcatttaa tataaaagat ttgacgtaat atgatgttag cgacatggac
    51961 aatatgatat tacgtcatgc ttgttttgtt tacttaaata taagctaagt aataagtaga
    52021 taattactaa caacaataac tagatagata agtgtaaatt tcttgtaaac aggtatataa
    52081 tagtatgtaa ttctttgaag gaagtaagtg aggaaggagt atgttgatgg ctaaatatcg
    52141 agggaaaccg tttcaattat atgtaaagtt atcgtgttcg acaatgatgg cgacaagtat
    52201 cattttaacg aatatcttgc cgtacgatgc ccaagctgca tctgaaaagg atactgaaat
    52261 tacaaaagag atattatcta agcaagattt attagacaaa gttgacaagg caattcgtca
    52321 aattgagcaa ttaaaacagt tatcggcttc atctaaagaa cattataaag cacaactaaa
    52381 tgaagcgaaa acagcatcgc aaatagatga aatcataaaa cgagctaatg agttggatag
    52441 caaagacaat aaaagttctc acactgaaat gaacggtcaa agtgatatag acagtaaatt
    52501 agatcaattg cttaaagatt taaatgaggt ttcttcaaat gttgataggg gtcaacaaag
    52561 tggcgaggac gatcttaatg caatgaaaaa tgatatgtca caaacggcta caacaaaaca
    52621 tggagaaaaa gatgataaaa atgatgaagc aatggtaaat aaggcgttag aagacctaga
    52681 ccatttgaat cagcaaatac acaaatcgaa agatgcatcg aaagatacat cggaagatcc
    52741 agcagtgtct acaacagata ataatcatga agtagctaaa acgccaaata atgatggttc
    52801 tggacatgtt gtgttaaata aattcctttc aaatgaagag aatcaaagcc atagtaatcg
    52861 actcactgat aaattacaag gaagcgataa aattaatcat gctatgattg aaaaattagc
    52921 taaaagtaat gcctcaacgc aacattacac atatcataaa ctgaatacgt tacaatcttt
    52981 agatcaacgt attgcaaata cgcaacttcc taaaaatcaa aaatcagact taatgagcga
    53041 agtaaataag acgaaagagc gtataaaaag tcaacgaaat attattttgg aagaacttgc
    53101 acgtactgat gataaaaagt atgctacaca aagcatttta gaaagtatat ttaataaaga
    53161 cgaggcagtt aaaattctaa aagatatacg tgttgatggt aaaacagatc aacaaattgc
    53221 agatcaaatt actcgtcata ttgatcaatt atctctgaca acgagtgatg atttattaac
    53281 gtcattgatt gatcaatcac aagataagtc gctattgatt tctcaaattt tacaaacgaa
    53341 attaggaaaa gctgaagcag ataaattggc taaagattgg acgaataaag gattatcaaa
    53401 tcgccaaatc gttgaccaat tgaagaaaca ttttgcatca actggcgaca cgtcttcaga
    53461 tgatatatta aaagcaattt tgaataatgc caaagataaa aaacaagcaa ttgaaacgat
    53521 tttagcaaca cgtatagaaa gacaaaaggc aaaattactg gcagatttaa ttactaaaat
    53581 agaaacagat caaaataaaa tttttaattt agttaaatcg gcattgaatg gtaaagcgga
    53641 tgatttattg aatttacaaa agagactcaa tcaaacgaaa aaagatatag attatatttt
    53701 atcaccaata gtaaatcgtc caagtttact agatcgattg aataaaaatg ggaaaacgac
    53761 agatttaaat aagttagcaa atttaatgaa tcaaggatca gatttattag acagtattcc
    53821 agatataccc acaccaaagc cagaaaagac gttaacactt ggtaaaggta atggattgtt
    53881 aagtggatta ttaaatgctg atggtaatgt atctttgcct aaagcggggg aaacgataaa
    53941 agaacattgg ttgccgatat ctgtaattgt tggtgcaatg ggtgtactaa tgatttggtt
    54001 atcacgacgc aataagttga aaaataaagc ataattatat tgggggaaga gcatctatat
    54061 atttttttaa gtatataaga cgtcttattt ccccttaatt tattgtgaag tatatgcaaa
    54121 atgcaatgaa tagattgtcc atcattttaa cgttataatg aatttaacga cttagaacta
    54181 cacaagtaaa ggagaatgaa gatgtctcga aaaacggcgc tattagtttt ggatatgcaa
    54241 gaaggtatag cgagtagtgt acctagaata aaaaatatta ttaaagcgaa tcagagagca
    54301 attgaagcag caagacaaca tcgaatacca gtcattttca tacgtttagt gttagataag
    54361 cattttaatg atgtctcctc gagtaataaa gtgttttcaa caattaaagc tcaaggatat
    54421 gcgattactg aagcagatgc atctacacga atacttgaag atttagcacc actagaagat
    54481 gagccgatta tttctaagcg acgctttagc gcatttacag gtagttactt ggaagtttat
    54541 ttacgtgcaa atgatattaa tcatttagta ttaacgggtg tctctacaag tggagctgta
    54601 ttgagcacgg cattagaaag tgtagataaa gactattata ttactgtttt agaagatgct
    54661 gttggtgata gatcagatga taaacatgac tttattattg aacaaatttt atcacgctca
    54721 tgtgacattg aatccgtaga gtcatggaaa agtagtttat agttaatata acgtcaatta
    54781 aagctcggca gtaatgtttg agaataagta catttgctca tatttataaa atgtgtgaga
    54841 tggcaattga aacggatatg atgaggaaca tttgaacata aaataatata tttatataaa
    54901 acgacccgag gcgttcgaac tgaatgcctc gggtttaatt gaataagaaa tcagacttat
    54961 gaacagaaat atgtttaagt ccgaactcct tgtttatact tataaatttt acgggtttaa
    55021 tataatactt atttacctgt aatatatgat aattcttcag cggcagctgc gttgatagtt
    55081 ctatgagaaa tgatacctaa tcctttaaca ttggattctg aaataacgat agaaccatca
    55141 ctgttaactt tttcaacaaa tgctacatga ccgtaatgtt gatctgcacc aaattgtcca
    55201 gcctcaaata caacagcagc atgacgtttt ggtgtatgac ttacttgata atcacggtat
    55261 tgagctcgat tattccaatt atgtgcatca cctaaatcac ctgagataga tgtaccaaat
    55321 tgtttcatac ggttatatac gtaccaagta cattggccat gtggatatgg catactatca
    55381 gatacctcac ggaaaggttt gaattcatct gatgaatcat cataatcctt gatagaacgt
    55441 tcatatttat ctaaatctgg catgcgttca tcgtcaaact gagttaattg atagtgttta
    55501 ataatactgt ttaatttctt agcatagttt ggatctgtag catatgtttt agataagtgt
    55561 gatgttgcat ctttataaga atcggcttcc gatttccatg ttggtttata aattgttcga
    55621 ttgccatcaa taccattttt aataaggtca gagtaatctt ttagtgattc tttcgtgctt
    55681 ggatattttc ggaatccagc attaatacta tacaattgat taccatcagc ttctaatgtg
    55741 ttaaaaggaa cagaattccc ttcaaaagca cctttgatac cgaataaatt atggtttggt
    55801 gacttagcta aagcactacg acctgagtca gattctaaga ttgcttgggc aatcatgaca
    55861 gacgcataaa tatcgttatc ttgaccaatg cgatgtgcat ctttagcaat tgatttgaca
    55921 aattgacgtg tatcttttga gtcaacaacg ttaaattgtc cgctatcatc attgttagat
    55981 atactaggat ctgtttcgaa taatgatgtt gcacgtgtat ccttttgatt aacatcgtta
    56041 ttgaatgatt gagcaggttt agatttatgt ttcaattcat cttgtgttgg taactgtgga
    56101 ttctttgtat tagatttttc atttttgtct tttttagatt gagatgcata atctttttgt
    56161 gctttctttg catcttcact gtattgatcc aaaatagagt ctaaagccga atctgacatt
    56221 gattgattat ctttcgatga agatttttga tttgctttat cgtcacttgc tggttgacta
    56281 tttgattgat taggttgtgt tggctttggc gaatttggtt gcttattaga tgtacttggt
    56341 tttgtattgt ttgatttagg tgctttttga ttgtctgctt tatcttgttt agatgattgc
    56401 gtatcagtgt catttttgat gctattgtca ctgtttttat tcgaatcatt tgttgacttt
    56461 tcgccattac gaggttgttc gtaatcagaa atatccgaat ttaaattgaa taagttttgg
    56521 attaaagttg ttaatgagta attatcatcg tatttatttt tggttagcaa ttggtttata
    56581 ttggtttgtg gtaaattctt ataaataaaa tcaatgatat tgttagagtc tgaagtgctg
    56641 tcgtctatag ttttaaattt tttgtcgtta ttgtcttggt tacttgtatt atttttgtct
    56701 gctttatcaa tatctttact tgtagtatcc ttagaagttt catcgtcatt agattttttt
    56761 gaatcatgag atgttgtctt agctgtagta tctttttgag gtgtatcagc ataagcggta
    56821 ggtgaaacta aagtaggtaa tacgagcgta gttgatagca aataaattaa aattttattt
    56881 ttaggcatat ttcgtattct cccttgaaaa atataataat taagtgtgat aataaactat
    56941 gatttgttat aatttatcgt atgctgaaaa tagttgatag gtatcaatcg actaaatatc
    57001 ttccagtaaa ttgattatac taattcacaa cgcaaaaata aattaattta caaaaaatat
    57061 ataaaaaata tgaataattc ctacatagga gtgtgacaat gaagaacgca tttaaattat
    57121 ttaaaatgga tctgaagaaa gtagctaaga cgccagctgt gtggattatc ttagcaggct
    57181 tagctatttt gccatcgttc tacgcttggt ttaacttatg ggcaatgtgg gatccatatg
    57241 gcaacacggg acacatcaag gtcgcagtcg ttaatgaaga taaaggcgac acaatcagag
    57301 ggaaaaaagt taatgtcggt aatacgatgg ttaatacact caagaaaaat aaaagttttg
    57361 attggcagtt tgtaagtaga gagaaagctg atcatgagat aaaaatgggt aaatattttg
    57421 caggtattta catcccatct aagtttacac atgaaattac agggacacta cgtaagcagc
    57481 ctcaaaaagc agatgtagaa tttaaggtga atcagaagat taacgctgtt gcgtctaagc
    57541 taacagatac tggttcgtca gttgtcgttg aaaaagcgaa tgaacaattt aataaaacag
    57601 taactcgagc attattagaa gaagctaaca aagcaggttt aactattgaa gaaaatgtgc
    57661 cgacaattaa caagataaaa aatgcggtat attcagcaga taaagcttta cctaagatta
    57721 atgactttgc gaataaaatt gtatatttga ataaccacca agcggattta gataaatatg
    57781 ccaatgattt tagaaaacta ggaaattata aaggtgatat tttagatgct cagaaaaaat
    57841 taaacgaagt caatggtgct attccgcaac ttaatgaaaa ggctaagttg atattagctt
    57901 taaataatta tatgccgaaa attgaaaaag cgttaaattt tgcagctgat gacgtgccag
    57961 cgcagttccc taaaattaat caaggactta acattgcgag tcaaggtatt gatcaagcta
    58021 atggacagtt aaatgatgcc aaaggcttcg tcacacaagt tagaagtaga gtcggtgatt
    58081 atcaagatgc aattcgacgc gcgcaagatt taaatcgaag aaaccagcaa cagattcctc
    58141 aaaatagcgc ggcgaacaac gaaacatcaa atagtgcacc tgcagctggt aatggtgtag
    58201 catcaacgcc accaagtgca ccaagtggcg atactgcacc aaataataat gttacgcaaa
    58261 ataccgcacc aaatagtaat aatgcgcctg tatcgactac accacaaagt acaagcggga
    58321 aaaaagatgg tcaaagtttt gtagatataa caacaacaca agtcagcaca gctaacgaga
    58381 acacacaaaa cattacagat aaagatgtta aatcaatgga agcggcatta acgggctctt
    58441 tattatcatt atcaaataat ttagataccc aagcgaaagc cgcacaaaaa gatagtcagg
    58501 cattacgtaa tatttcgtat gggattttag catcggacaa gccttctgat tttagagagt
    58561 ctttagataa tgttaagtcc ggtttagaat acacaacgca atataatcaa caatttatcg
    58621 atacattaaa agagattgag aagaatgaaa atgttgattt atcaaaagaa attgataagg
    58681 taaaagcagc taataatcga attaatgaat cattaaggtt agttaatcaa ttaagcaatg
    58741 cattaaagaa tggtagttca ggaactgctg aagctactaa attactagat caactttcaa
    58801 aactagattc atcattatca tcatttagag attatgttaa aaaagatctt aacagctctt
    58861 tagtatcaat atcacaacgt attatggatg aattgaacaa agggcaaact gcattatcca
    58921 atgttcagtc taaattaaat acaattgatc aagtcattaa cagtggacaa gctattttaa
    58981 aaaatggtaa aacacgtatt gatcgcttac aaacagtatt accaagtatt gaacaacaat
    59041 acattagtgc tgttaaaaat gctcaagcaa acttctcgaa agtgaaaagt gatgtagcta
    59101 aagctgctaa ctttgtgcgc aatgacttac cacagttaga acagcgatta actaatgcga
    59161 cagcaagtgt gaataaaaat ttaccaacgt tattaaatgg ttatgatcaa gcggtaggat
    59221 tactaaataa aaatcagcca caagcgaaaa aggctttatc agatttagct gatttttctc
    59281 aaaataaatt gcctgatgtt gaaaaagatt tgaaaaaagc gaataaaatt ttcaagaaat
    59341 tagacaaaga tgatgcagtc gacaaattaa tcgacacact taagaatgat ttgaaaaagc
    59401 aagcgggtat tattgcaaat cctattaata agaagactgt tgatgttttc ccagttaagg
    59461 attatggttc aggtatgaca ccattctata ctgcactgtc agtatgggta ggtgcactct
    59521 tgatggtaag tttattaacg gttgataata aacataagag tctagagtca gtcttaacga
    59581 caagacaagt gttcttaggt aaggcaggat tctttataat gcttggtatg ttgcaagcac
    59641 tcattgtatc ggttggagat ttgttaatcc taaaagcagg agttgagtca cctgtattat
    59701 ttgtacttat aacgattttc tgttcgatta ttttcaactc aatcgtatat acgtgcgtat
    59761 cattacttgg taacccaggt aaagccattg caatcgtatt gcttgtatta caaattgcag
    59821 gtggtggggg aacattccca attcaaacta cgccacaatt tttccaaaac atttcgccat
    59881 acttaccatt tacgtatgca attgattcat tacgtgaaac agtaggcggt attgttccgg
    59941 aaatcctaat tacaaaatta attatattaa cgttatttgg tataggattc ttcgttgtag
    60001 gtttaatttt aaaacctgta acagatccat tgatgaagcg cgtatctgaa aaagttgacc
    60061 aaagtaacgt tacagaataa aaattaaatc cacacattag ggttatagct ccttaatgtg
    60121 tggattttta tgtttttaga cagaagagat agtaatttct gtcttttatg ggacggttgt
    60181 tatcattgct attatccagg atgacttact atagcactaa tattaccgac aaagtgaata
    60241 tcctcgtctt ccgtagttaa aataaagcta gaaccttttt ggatgtcata gtgcttatcg
    60301 tttactgtta aagtaccagt accatcgata attgtaacta agcaataagc atgtggttta
    60361 ttgaatttta aatctccatg aatatcccat ttatatactg caaaatattg attatctaca
    60421 aattgagtta cagtgtgtgt gtcgatgtga gttgttatag gagtagtatt tggttcatga
    60481 ttgcctaatt caatcacatc tttactttgc tctaagtgca aatcacgcaa ttgaccattt
    60541 tgatctcgtc tatcatagtc ataaatacgg tatgtcgtat cggaggattg ttgtgtctct
    60601 aaaattaaaa tacccgaacc aatggcatgg acagtgccag caggaacata ataaaagtca
    60661 ccgggcttaa caggtatacg tttgaaaaga ctgtcaaatt catgattatc aatcatgtct
    60721 attaacgtct gtttattatg tgcatgtacg ccatatataa tttcagcacc tggggctgca
    60781 tctaaaatat accaacattc tgttttacct agttcgcctt cgtgttttaa agcgtagtca
    60841 tcatctgggt gaacttgaac agataattta tcattggcat ctaatacttt agttagcaga
    60901 gggaaactat ctcgtgaatc attatcgaat aattcacgat gttgtgacca aagttgatct
    60961 agggtcatat ccttgtatgg accattgata attgtattag gaccatttgg atgtgcagaa
    61021 attgcccagc attcaccagt tgtttcatta gggatatcat agttaaatgc ttttaatgca
    61081 tgaccgcccc aaattctgtc tttaaaaacg ggttgtaaaa ataatgccat agttaaaact
    61141 cctctatatt ttcattaata agttataaat ttctgtagta ctgtttgcat taattagtga
    61201 ttggcgtgtc tcatcattca ttaacgcttt agataagcgc tgaagtattt ttaaatgtgt
    61261 atcctgactg ttgtttggta cggcaattaa gaatatcaat tgaggtagac taccatctag
    61321 actgtcccat ttaacaccat gattattttt cataacagct acaatcggtt gttttacaac
    61381 atcagacttt gcatgtggaa tggccacgtt catgccaata gctgtcgtag accccatttc
    61441 acgttctagt attgcatttt ttaaatgcga tgtgtgctct acataacggc aaattttaag
    61501 tttatgaatc aacatatcaa ttgcttcgtt tcgagacatg tcgtgatcag taattatcat
    61561 agtttgttga tcaaaaacat gagaaggttt attgagatgt gaatgtttcg cggtgttatc
    61621 tacattgtca acctctgtat catgttgtgt aatatctgta tcatgaagtt gcgtgtgttg
    61681 cgctggtgca tctactgcta taactggtgt attgcgtttt aataatagta cagtagtcat
    61741 tgtgacaaga ctacctacta tcactgcaaa gataaaccat aatacatgat caataccacc
    61801 taatacagcc acgattggac ctccatgtgc gactctatcg ccgacaccac caatggctgc
    61861 aatgactgat gcaatcattg caccaatgat gtttgcaggt ataatgcgca atggatcttg
    61921 ggctgcgaaa ggaatagcac cttcagtaat accaaatagt cccatagtga aggaagcctt
    61981 acccatttct ctttcggaat gattgaattt atacttttga acaaacgttg ctaaacctaa
    62041 accgattggt ggtgtacata cagcaactgc gaccataccc ataacggcgt aattaccttc
    62101 agcaataagt gctgagccaa ataaaaatgc taccttgttt actggaccgc ccatatcgaa
    62161 ggcaatcatc gcacctataa tcatcgcaag tataataata ttagcacctt gcatactttt
    62221 taaccaggtt gttaatgcct caaaaatatt agaaattggt gcaccgatta aaaatataaa
    62281 tatcaatcct acaacgaccg atgaaataat gggaataata atgataggca taattggtgc
    62341 cattgctttt ggaactttaa tatctttaat ccactttgcg atataacctg ctaagaaacc
    62401 agcaacaata ccacctaaaa atcctgcgcc tgcatcactg ccataaaaac taccgtcagc
    62461 agcgatagcg ccgccaatca taccaggaac aagaccgggc ttgtcagcga tactaacagc
    62521 gatatatcca gctagtattg gaaccataaa tttaaaggct aaactaccaa tgttttcaat
    62581 ggatttccaa aatgaatcat ctgggatgac taatcctttt gatgtcgttt caccgcctag
    62641 agtcagcgcg atggcgataa ggagtccacc aactacgata aaaggaacca taaacgatac
    62701 accgttcatt aaatgttgat acaccatttg aataccactt ttagacttac cgcgatcttt
    62761 cgaatgataa tttgtttcag attgataaat aggcgcatct tgattaatga tacgttgaat
    62821 tagacctctc ggattatgaa tcccttcgcg aacattttca ttaatcaacc gtttaccaac
    62881 aaatcgggac agatcaactt gtttatcagc tgcaattatg acaccgtcag cttcttcgat
    62941 gtcttgcgta gttaaaacat tttcagcacc aacaccgccc tgtgtctcta ctttaatatc
    63001 cacacccatt tcttttgcta cctgctcaag cttttcttga gccatatatg tatgtgcaat
    63061 gccatttggg catgaggtaa tagctacaat tttcataaaa tcatctcctt ttctatattg
    63121 taagcgtatt ctcgatacta aaaaaaagaa taattaccgt tactagtggc aattattctt
    63181 gtaagtattc aaataactgt tgctttaaac tatgatcatc taaactacat aaatggttca
    63241 ctgaatcatc atccaagtta gcaattaatt gcatcatttg ttttgtaaaa gctttgtctt
    63301 tatgcgaaat cgctaagaaa aagacaagtt tgacatcgtg ttgtcgccaa ggaaaaacat
    63361 cttttgtgcg aaaaataagc acatgtgatt gtaaaacttt ttcaggatct ccatgaggaa
    63421 tcgccataaa attacctatg tatgtagaag atgatttctc acgctctaaa gctgattcga
    63481 tatatccttc tacaatcgca tgatgtgctt gtaatatttt ttgagcttct tcaaaaattt
    63541 gcacagtatg ccgtgatttt tgttcagtat ttacgacaag gaaattgaca gtgtccatat
    63601 gatgatgtgc ttgaaccgga ttttgctttt gcttcacaac gtgtctgatt ttgtgacgat
    63661 catcttcaga aaataatggt gcaaccttga tagtcgtcag gtgcttagga agtatgttta
    63721 gcgtttgttt aggaatatca tgggtcgtta ttaataaatc tacattgtca aagtgatagt
    63781 gtgttatatt ttctagttta atcgtattta tcactgacaa ctcttcggat aagttattta
    63841 ttttagtttc taaaaaattc gacacaccta gaccataata acaagcaatg actacattta
    63901 attgtgtttt ggtacgacgc tcgatggcag cttgaaaatg aattgttaaa aatgcaattt
    63961 catcttcgct catctctata tcagtatcaa ttgctaattt atcaatcgct tcaaaaagtg
    64021 tgttaaacac aaagggatag agttttttaa tctctataac taaaggattg tttaaataaa
    64081 tgttttgagt gatacgtaaa tatgctttac taaaatgatt atataaattt tgttgtaaaa
    64141 tcgaatcttc attgaaaggt acatgaatac gttgctgcat caattcgatt aagcgatcaa
    64201 tataactttg tataaatata cgttctatgc caatatcgag tttattaaaa tgataagcaa
    64261 taaagaatga aaacatattg attacttttt cgttcaagtc ataacctaat ctttcgttga
    64321 tttgcttaat gcaagattga gatatcaatt ttgaatcgtg gtttaacgtg tctagctcac
    64381 ttaaagattg cttacgttta atgattaaaa tgagatgaat taaaagctgt tgtatttgaa
    64441 tatcagttgt ttcaatacta tgttgttgaa gtgtctcttg tataatatgc gaaatcatcc
    64501 tttggtgtga atcaggtaat tcatttaaaa ttaggtcttc aacatgtaca tgccctgatg
    64561 ataattgatt taaatggatg atggcattag tgatatcatt atctgttcca tcgaccacaa
    64621 taccttgttt tttcttgatt gttaaagcga tattaaattt ttgacaccat tgttgaattc
    64681 gaacgaaata gtcgttgagc acagttttag ataatagata atcttgctct agttgttgca
    64741 atgtctgcga tttattgtgc attagcagtt gatacgccat agataataat agtttttctt
    64801 tatatgattg aatatgcgtg taacgttcag ttatcgtttc taaggtatac tgagatgtat
    64861 ttaattgata tcctttagat ttaatactta cgatgatatc atccataaaa ttgctattga
    64921 taacatggat atcattgcga actgtgcggt ttgaaacatt gacatgttta gcaatttcat
    64981 tagaactaat gtgctttgat ggatttttaa taaaaaactg gagtagtttt aagtgtctat
    65041 caagcattta acatgtacct cctttctaaa tttttcgtgt aagcgttttt aagggtgtat
    65101 taatattatt aaacatgaga gcttatacat acgtcaatga cattaaagcg aacttttata
    65161 tgattttaac agagtgcgaa ttatgcaaat aaagaacagc agtaagatat ttcaaataga
    65221 aaaatatctc actgctgttt ttctgaattt atgcatctgg tacttgtgga cgtatcaggc
    65281 aaatgattaa tttttaggtg attgtgcttg aggtgtttgt ttagaaggcg tattggtatt
    65341 atttgattta ttagcaggtg gtgtgttctg ttgttgatta ttttggttgt ttgttgacga
    65401 ttgactgcca ccaccgtgag tattattttt atttgaattt gagtcttttg gctgagctgg
    65461 ttgcgtattc gatggtactg ttgaatggtt gttaggaaca tttgacggtg aagatggttg
    65521 attgtcatta gtaacgttat tgccattatt ttgattttta tctgttttgt ctgcactatc
    65581 atcttgttga tcattttctt cggtttctgt ctttttatgc gtagatttat tttcttgttc
    65641 ttggttatta cttgatgatg tagaatgatt tattgcagca taaattgcga acccaagtgc
    65701 tccgattaat acgataatta cgatggtaat aagaagtttt ttcaaatata acgcctccat
    65761 gttaatgaaa agtagttgat acgtgaagct aaataattta tttcaatatt gccataatca
    65821 ttttaaaaaa taaatattag aaaagtaggc gatattaata ttttcattgt tttttgtgta
    65881 cttcaattag tataacaaaa ctgttatgta tttataaata taatcattag tatttttatg
    65941 gctgaaaaag ttataataaa agtgtaagga ataaaatatt tgtatggaaa agagagataa
    66001 ttatgaataa aaccagtaaa gtttgtgtag cagcaacatt agcattgggc acactgattg
    66061 gagtaacagt tgttgaaaat tcggcgccaa caagtaaaca ggcacaggca gcaataaccc
    66121 catattatac ttataatggt tatattggta ataatgctaa ttttattctg gataagaatt
    66181 ttattaacgc gattaagtat gataatgtga aattcaatgg tattaaatta gctaaaacga
    66241 atacgataaa aaaagtagag aaatatgatc aaacttttaa aggtgtttct gcaaaaggaa
    66301 acgaagcaag tcaattgcaa tttgtagtta aaaataatat ttcattaaaa gatatccaaa
    66361 aagcttatgg caaggacttg aaaaaagaaa atggtaaaac aaaggaagct gatagcggta
    66421 ttttttacta tcaaaatgct aaaaagacat taggcatctg gtttgtcgtt gatcataata
    66481 gagttgtcga agtaacagtt ggacatacac catacaaaac aagtaaataa aataatggca
    66541 tattaaggct agagtgtgag gagtgatacc gcattctagt cttttttatt aaataataac
    66601 gattattgcg tcttacatag ttgtttgaaa ttgataaaaa tgtttatttg attatgatta
    66661 tttaaatagt tatctcacat atttcaagca ttgattaagt atatagagca ctattttgta
    66721 tttgttaata ttttcacaaa aataaagcct tgataaattt taaaatataa ttaagctcaa
    66781 tttttaaaat tttatttagc tacagataac atttttaaaa aagaaaagaa tcaataaata
    66841 aaatcaacga acaaaaagta tagaaataaa tagaaataat catttacttt tctgaaaaat
    66901 taaattaata ttttatttat aagctgtttt taagatttca ggaggaatga aatgtgagga
    66961 aattttcaag atatgcattt acaagtatgg cagcattaac cttgttgagc actttatcac
    67021 cagcagcatt agcgattgat tcaaaaaata aaccagctaa ttctgatatt aaatttgagg
    67081 tgactcaaaa gagtgatgcg gtcaaagcat taaaagaatt gcctaaatcc gaaaatgtaa
    67141 aaaatattta tcaagattac gctgttactg atgtaaaaac tgataaaaaa ggatttacgc
    67201 attatacatt gcaaccgagt gttgatggtg ttcatgcacc tgacaaagaa gtgaaagtac
    67261 acgcagacaa atcaggaaaa gtcgttttaa tcaatgggga tactgatgcg aagaaagtaa
    67321 agccaacgaa taaagtgaca ttaagtaaag atgacgcagc cgacaaagca tttaaagcag
    67381 ttaagattga taagaataaa gcgaaaaatc ttaaagataa agtcattaaa gaaaacaaag
    67441 ttgaaatcga tggtgacagt aataaatacg tttataatgt tgagttaatt acagtgacac
    67501 cagaaatttc acattggaaa gttaaaattg atgctcaaac tggcgaaatt ttagaaaaaa
    67561 tgaacttagt taaagaagct gcagaaactg gtaaaggaaa aggtgtactt ggcgatacaa
    67621 aagatatcaa tatcaatagt attgacggtg gatttagcct agaagattta acgcatcaag
    67681 gtaaattatc agcatttagc tttaatgatc aaacaggtca agcaacattg attactaatg
    67741 aagatgaaaa cttcgtaaaa gatgagcaac gtgctggcgt agatgcaaat tattacgcta
    67801 aacaaacata tgattattac aaagacacat ttggtcgtga atcatatgac aaccaaggta
    67861 gtccaattgt ttcattaacg catgttaata actacggtgg tcaagataac agaaataatg
    67921 ccgcatggat cggtgacaaa atgatctatg gtgatggtga tggtcgcaca ttcacaagtt
    67981 tatcgggtgc aaatgacgta gtagcacacg aattaacaca cggtgtgaca caagagacag
    68041 cgaacttaga atataaggac cagtcaggcg ctctaaatga aagcttttca gatgtttttg
    68101 gatactttgt agatgacgag gatttcttaa tgggtgaaga tgtctacaca cctggaaaag
    68161 agggagacgc tttacgcagc atgtcaaacc cagaacaatt tggtcaacca gctcatatga
    68221 aagactatgt attcactgaa aaagataatg gtggcgtaca tacgaattct ggaattccaa
    68281 ataaagcagc ttataacgtg attcaagcaa tagggaaatc taaatcagaa caaatttact
    68341 accgagcatt aacggaatac ttaacaagta attcaaactt caaagattgt aaagatgcat
    68401 tataccaagc ggctaaagat ttatatgacg agcaaacagc tgaacaggtg tatgaagcat
    68461 ggaatgaagt aggcgtggag taaaaatata taaacaagaa gaagtaatgt taaacactta
    68521 taaataatta aattttaaat acagcttata tatggggtat acctacattg ataattgact
    68581 ttgtaggtat accctctctt tgtaattaga tgagaggagt gtgagggttg tctgccgaaa
    68641 gactactcgg cagtctaaaa tcattacaag tagtagatat gtgataatta aatgctgact
    68701 tagaatacaa aattcatttt aaaagttgtc acaaaaaatt tacatgtatt tttattatat
    68761 tttgcaaaac aaagtgttaa attataaatg aaacatgcat gaatttattt tttaatacaa
    68821 gaaacgtaac taccaaagga gtttacaata tgaagaaaag taaacgatta gaaattgttt
    68881 ctacaatagt taaaaagcat aagatttata aaaaagaaca aatcatttca tatattgaag
    68941 aatattttgg tgtaagatat agtgcaacaa caattgctaa agacttgaag gaactaaata
    69001 tatatcgtgt ccctatcgat tgtgagacat ggatttataa agctattaat aatcaaacag
    69061 aacaagagat gagagaaaag tttagacact attgtgaaca tgaagttcta agttcaatca
    69121 tcaatggttc atacattatc gtcaaaacct cacctggttt cgcccaaggc ataaactatt
    69181 ttatcgatca gctaaatata gaagagatat taggtacggt gagtggaaat gacactacat
    69241 taatcttaac tgcctcaaat gatatggcag aatacgtata tgcaaaatta tttaaataga
    69301 catgtatcaa atgaataata aaaatttgtt tcgtatcacg tgtactcaag ttagttacca
    69361 aatattaact tgtgtacgcg tttttttatg gaaagaaaga attcatagtc attcaattga
    69421 ctgtataaaa aactttatac aacatgtttt tatgggtgtt t
//
LOCUS       DAKTGS010000003        65764 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.003, whole genome shotgun sequence.
ACCESSION   DAKTGS010000003 DAKTGS010000000
VERSION     DAKTGS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 65764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65764
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.003"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(96..1265)
                     /locus_tag="O0286_000130"
     CDS             complement(96..1265)
                     /locus_tag="O0286_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HCY0168534.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(1693..3387)
                     /locus_tag="O0286_000131"
     CDS             complement(1693..3387)
                     /locus_tag="O0286_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0168535.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(3409..4434)
                     /locus_tag="O0286_000132"
     CDS             complement(3409..4434)
                     /locus_tag="O0286_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0168536.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(4427..5143)
                     /locus_tag="O0286_000133"
     CDS             complement(4427..5143)
                     /locus_tag="O0286_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCY0168537.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(5671..6714)
                     /locus_tag="O0286_000134"
     CDS             complement(5671..6714)
                     /locus_tag="O0286_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0168538.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(6738..6884)
                     /locus_tag="O0286_000135"
     CDS             complement(6738..6884)
                     /locus_tag="O0286_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168539.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(6886..7941)
                     /locus_tag="O0286_000136"
     CDS             complement(6886..7941)
                     /locus_tag="O0286_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCY0168540.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            complement(7959..9218)
                     /locus_tag="O0286_000137"
     CDS             complement(7959..9218)
                     /locus_tag="O0286_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCY0168541.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(9445..9723)
                     /locus_tag="O0286_000138"
     CDS             complement(9445..9723)
                     /locus_tag="O0286_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0168542.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(9746..10213)
                     /locus_tag="O0286_000139"
     CDS             complement(9746..10213)
                     /locus_tag="O0286_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0168543.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(10198..12294)
                     /locus_tag="O0286_000140"
     CDS             complement(10198..12294)
                     /locus_tag="O0286_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0168544.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(12425..12523)
                     /locus_tag="O0286_000141"
     CDS             complement(12425..12523)
                     /locus_tag="O0286_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168545.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            complement(12633..13568)
                     /locus_tag="O0286_000142"
     CDS             complement(12633..13568)
                     /locus_tag="O0286_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0168546.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            13930..15459
                     /locus_tag="O0286_000143"
     CDS             13930..15459
                     /locus_tag="O0286_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0168547.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(15779..16732)
                     /locus_tag="O0286_000144"
     CDS             complement(15779..16732)
                     /locus_tag="O0286_000144"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0168548.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(17043..17150)
                     /locus_tag="O0286_000145"
     CDS             complement(17043..17150)
                     /locus_tag="O0286_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168549.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            17305..18450
                     /locus_tag="O0286_000146"
     CDS             17305..18450
                     /locus_tag="O0286_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCY0168550.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            18476..18649
                     /locus_tag="O0286_000147"
     CDS             18476..18649
                     /locus_tag="O0286_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168551.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            18806..19162
                     /locus_tag="O0286_000148"
     CDS             18806..19162
                     /locus_tag="O0286_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCY0168552.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            19360..20835
                     /locus_tag="O0286_000149"
     CDS             19360..20835
                     /locus_tag="O0286_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0168553.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(21149..22291)
                     /locus_tag="O0286_000150"
     CDS             complement(21149..22291)
                     /locus_tag="O0286_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCY0168554.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            22732..24309
                     /locus_tag="O0286_000151"
     CDS             22732..24309
                     /locus_tag="O0286_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCY0168555.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            24335..25840
                     /locus_tag="O0286_000152"
     CDS             24335..25840
                     /locus_tag="O0286_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCY0168556.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            25952..27163
                     /locus_tag="O0286_000153"
     CDS             25952..27163
                     /locus_tag="O0286_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCY0168557.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            27349..29610
                     /locus_tag="O0286_000154"
     CDS             27349..29610
                     /locus_tag="O0286_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCY0168558.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            29640..30824
                     /locus_tag="O0286_000155"
     CDS             29640..30824
                     /locus_tag="O0286_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0168559.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            31138..31245
                     /locus_tag="O0286_000156"
     CDS             31138..31245
                     /locus_tag="O0286_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168560.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(31427..33256)
                     /gene="coa"
                     /locus_tag="O0286_000157"
     CDS             complement(31427..33256)
                     /gene="coa"
                     /locus_tag="O0286_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCY0168561.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNV
                     TTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            33446..33790
                     /locus_tag="O0286_000158"
     CDS             33446..33790
                     /locus_tag="O0286_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCY0168562.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            complement(33954..35717)
                     /locus_tag="O0286_000159"
     CDS             complement(33954..35717)
                     /locus_tag="O0286_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCY0168563.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(36053..36808)
                     /gene="pflA"
                     /locus_tag="O0286_000160"
     CDS             complement(36053..36808)
                     /gene="pflA"
                     /locus_tag="O0286_000160"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCY0168564.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(36831..39080)
                     /gene="pflB"
                     /locus_tag="O0286_000161"
     CDS             complement(36831..39080)
                     /gene="pflB"
                     /locus_tag="O0286_000161"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCY0168565.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            39668..40636
                     /locus_tag="O0286_000162"
     CDS             39668..40636
                     /locus_tag="O0286_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0168566.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            40633..42189
                     /locus_tag="O0286_000163"
     CDS             40633..42189
                     /locus_tag="O0286_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0168567.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            42182..42940
                     /locus_tag="O0286_000164"
     CDS             42182..42940
                     /locus_tag="O0286_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0168568.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(43299..44678)
                     /gene="uhpT"
                     /locus_tag="O0286_000165"
     CDS             complement(43299..44678)
                     /gene="uhpT"
                     /locus_tag="O0286_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCY0168569.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            44911..45405
                     /locus_tag="O0286_000166"
     CDS             44911..45405
                     /locus_tag="O0286_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCY0168570.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(45767..46735)
                     /locus_tag="O0286_000167"
     CDS             complement(45767..46735)
                     /locus_tag="O0286_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCY0168571.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(46790..47830)
                     /locus_tag="O0286_000168"
     CDS             complement(46790..47830)
                     /locus_tag="O0286_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0168572.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(47855..48931)
                     /locus_tag="O0286_000169"
     CDS             complement(47855..48931)
                     /locus_tag="O0286_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0168573.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(49005..49844)
                     /locus_tag="O0286_000170"
     CDS             complement(49005..49844)
                     /locus_tag="O0286_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0168574.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(49846..51114)
                     /locus_tag="O0286_000171"
     CDS             complement(49846..51114)
                     /locus_tag="O0286_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0168575.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(51117..52388)
                     /locus_tag="O0286_000172"
     CDS             complement(51117..52388)
                     /locus_tag="O0286_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0168576.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(52401..53498)
                     /gene="ugpC"
                     /locus_tag="O0286_000173"
     CDS             complement(52401..53498)
                     /gene="ugpC"
                     /locus_tag="O0286_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCY0168577.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(53881..54459)
                     /locus_tag="O0286_000174"
     CDS             complement(53881..54459)
                     /locus_tag="O0286_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0168578.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            54668..55294
                     /locus_tag="O0286_000175"
     CDS             54668..55294
                     /locus_tag="O0286_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCY0168579.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            55484..56257
                     /locus_tag="O0286_000176"
     CDS             55484..56257
                     /locus_tag="O0286_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0168580.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(56555..58561)
                     /gene="ggt"
                     /locus_tag="O0286_000177"
     CDS             complement(56555..58561)
                     /gene="ggt"
                     /locus_tag="O0286_000177"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCY0168581.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(58599..60374)
                     /locus_tag="O0286_000178"
     CDS             complement(58599..60374)
                     /locus_tag="O0286_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0168582.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(60391..61554)
                     /locus_tag="O0286_000179"
     CDS             complement(60391..61554)
                     /locus_tag="O0286_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0168583.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(61560..63038)
                     /locus_tag="O0286_000180"
     CDS             complement(61560..63038)
                     /locus_tag="O0286_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0168584.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            62995..64587
                     /locus_tag="O0286_000181"
     CDS             62995..64587
                     /locus_tag="O0286_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0168585.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(64852..>65764)
                     /locus_tag="O0286_000182"
     CDS             complement(64852..>65764)
                     /locus_tag="O0286_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCY0168586.1"
                     /translation="LPDTFSPKEGKKIEIALKDDWSIFNVEEAQISCILNELFKDDEN
                     LDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNMINLENLAFF
                     LDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLNSKWISVLYL
                     STKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIKAGDDGFRKY
                     FVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESILQDGTFNFVF
                     FDQSLFECVNYERKRVSDVKYTLTKLS"
BASE COUNT        21086 a        12214 c        10417 g        22047 t
ORIGIN      
        1 tttcatacac aaataataac atacaagcaa acatttcgtt tattatttat atcacttaac
       61 taattaattt ataatttttt attgttttta agttatcact taaaaatcgt ttggcaaatt
      121 cgttgtgacg cttgtccatc ttctaatgaa cagaattttt gataaaatac cgttcgtgct
      181 tcaatatact catttgcagt ctcatcgatt tgttttaatg catcaatgag tgctgtttga
      241 ttttcaacaa ttggacctgg caactctttt ttataatcca tgtaaaaacc tctaagctca
      301 tcgccatatt tatctaagtc atatgcatag aaaatttgcg gacgctttaa tacaccgaag
      361 tcgaacatga cagatgagta gtcggtaact aacgcatcgc tgattaagta taaatccgaa
      421 atgtcttcat aatctgaaac gtctttcaca aaatcatcat gttcatcaat acgtgtcaca
      481 actaaataat gcatgcgtaa taaaataaca taatcatcat ccagcgcttg acgcaaagct
      541 tctatatcaa agttaacatt aaattgatat gaaccttctc gaatcgcttc atcgtcacgc
      601 caagttggcg cgtacataat cactttttta tctaatggaa tatttaatct tgtcttaata
      661 ccattaatat attcagtatc attgcgttta tgtgataatt tatcatttct tggataacct
      721 gtttccaaaa tcttatctcg actaacatga aatgcatttt gaaatatcga tgtcgaatat
      781 ggattaggtg acactagata atcccaccgt tggctttctt ttttaaagcc atcttggtaa
      841 ttttgagtat ttgttcctag cattttaacg ttactaatat ccaaaccaat cttttttaat
      901 ggcgtgccat gccatgtttg taagtacgtc gttcgcggtg atttatataa ccaatctggt
      961 gtacgtgtgt taatcatcca cgctttcgct cttggcatcg ctaaaaacca tttcattgaa
     1021 aactttgtaa catatggtac attgtgctgt tggaatatgt gttcatatcc ttttttcaca
     1081 ccccatatta attgggcatc gctatgttca gttaagtatt catataatgc tttggggttg
     1141 tcgctgtatt gtttaccatg aaagctttca aaataaatta gattcttgtt tggcaatttt
     1201 gatagtaatt taaaagtcgt atatatacta tgttctatca attttttaat tgtattttta
     1261 atcatgtcgt acctccgacg tgtttttgta attatattaa tatgtatgag caagctcatt
     1321 gtaaccatgc ctattatagc atttcatcat aaaatacatt taaccattac acttgtcgtt
     1381 aattatcata cgaaatacat gattaatgta ccactttaac ataacaaaaa atcgttatcc
     1441 attcataacg tatgtgttta cacatttatg aattagataa cgattggatc gattatttta
     1501 ttttacaaaa tgacaattca gttggaaggt gattgctttt gattgaatcg ccttatgcat
     1561 gaaaaatcaa aaggttattc tcattgtata gtcctgcttc tcatcatgac atgttgctca
     1621 cttcattgtc agaacccttc ttgaaaacta tgccttatga ctcatttgca tggcaagtaa
     1681 tatatgccaa cattagcgtc taaacaaatc tttgactaaa cgttcacttg agcgaccatc
     1741 ttgatattta aaatgtttat ctaagaatgg cacaactttt tcaacctcat aatcttcatt
     1801 gtccaaagca tccattaatg catcaaagga ctgtacaatt ttacctggaa caaatgattc
     1861 aaatggttca tagaaatcac gcgtcgtaat gtaatcttct aagtcaaatg catagaaaat
     1921 catcggcttt ttaaatactg catattcata tattaaagat gaataatcac taatcaacaa
     1981 gtctgtaaca aagagaatat cgttaacttc acgatgatct gacacatcga taaagtattg
     2041 tctatgttca cgtgaaatat taagtctatt ttttacgaac ggatgcattt tgaataacac
     2101 aactgcatta tgcttctcgc agtatcttgc taaacgttca aaatcaattt taaaaaatgg
     2161 ataatgtgcc gtaccgtgac cattacctct aaatgtcggt gcgaatagaa taactttctt
     2221 accttttata attggcaatt catcttccat ctcttgttta atttgtgttg cataagcttc
     2281 atcaaatagt acatcagtac gtggtacacc tgttggtaca acattttctt ctctaattcc
     2341 aaatgcttca gcataaaatg gaatatcggt ttctgatgaa acatatgctt tcgtgtaact
     2401 acgatggttt aatgaatcga taaacggacc acctttttta cctgtacgac taaagccaac
     2461 tgttttaaag gcaccaacag catgccacac ttgaataatt tcttgtgatg gtctaaagcg
     2521 aaccgtataa attaatggat ggaaatcatc aacaaaaata taatcggcct taccaagtaa
     2581 atatggcaat ctaaacttgt ctctccattt gcgtctatcc gtaatattct ccttaaatac
     2641 cgttttaata tcataatcaa aatctacttt ttggcgtagt aactcatcat atacatactt
     2701 gaaattccct gataaattcg gacgcgaatc tgatgtgaat aatattgttt tgcttctttt
     2761 aatatgtagt aactttgtaa tattaaaaat agctttaaat aagaaacttc tactttcaaa
     2821 tgaagcttta tgaccttgtt tatgaagcca gtgtgcactt gtcgcaatga cccctgattt
     2881 ctcttgaggt aaggtgattt caatatcaaa tacaaattcg ttaacgtcac ttgctatttc
     2941 cggagtaatg gtatagaccg tatgttgtga tacgccacct ttacggaaaa ctttagcatc
     3001 atacgctaat aaatagttca cttttccatt ttgtgtagtc agctcattgt attcttcaat
     3061 ttgctcttca tttaatgccg cataagcttg atcaataata tttggattaa gttgtccaat
     3121 atactctaac tggtcatgat aaacaaataa atatttatcc attggtaaat aatacccatc
     3181 gtctaatatt gcgacgttga atctacatat aaatgtatta ccgtctattt tcatttcatt
     3241 tgcttctaat gtttttgttt cggttaaatt cctcagaaca aattgtcctg atgttaaatc
     3301 gacaccttca ctatgtcctt caatatataa ctgaacacgt tcccagtata tgttatcaat
     3361 atgtattgct tgtttcgttt ttgtcattgt tattccccca tcggtttatt aaatcgtcca
     3421 tttcaatact gtttttcccc aagatgtcga taaatccatt tcaaacgctt ggacgatatc
     3481 ttgcatcgta catacattaa tttcatgtcc ttttaataat gctaactttt caactatgtc
     3541 tgggtactta cgatataaat caacaacttg ctcaaaatct ttagagccgc ttcgactact
     3601 accaatcaac gttaatcctt tttcaagtac taatcgtgta ttcacttcca cgggtaattc
     3661 acttacgcct aacaaagcaa tactgccttc tggtgaaata tgttcaacta tttgttgaag
     3721 tgcaacttga cttcctttac ctccaacaca ttcaaatgca tgatcaattt taagatcatc
     3781 tggtatttga tttactgtaa agatgtcatc tacaaatgaa aaatgactta atttatagtc
     3841 tgtcttacca aatacataag ttttagcttc tgggtacaac ttacgtagca aaatagcagt
     3901 aatataacct aagttaccat caccccaaat accaaagctg gttttcaaag gtatagattt
     3961 acgttcaaat cgttgtatag catgataact tactgacact aactctgtgt atgaaatcgt
     4021 actcaaatca atgtcattag gcagcggaac gatacgatca tgtgccatca caacgtagtc
     4081 ttgcataaaa ccatcataac cactagatct aaaataacta gaggctaagt aattctccgc
     4141 aataatatga tgttgctctg taggtgtatt cggtaccatt actactttcg tacctttttc
     4201 aaatacccct ttactatcaa atacaacttc accaacagct tcatgaacta atgacattgg
     4261 taattttttg cgtagtacat tttcatctct tcgacctgtg taataccttt gatcagctgc
     4321 acaaatagac aagtataaag gtcttacgat gacatgatta ccataaatat caacattatt
     4381 atatgtgacg tcgaactgtc tcggtgcaac gagttgatat acttgattaa tcatcggcaa
     4441 tatcaccttg aataatggca tttgctactt ttaaatcata cggtgttgtc actttaatgt
     4501 tgtatagttc tccacgtacc aatttaactg catgtccaga ttcgacaatg attttacatg
     4561 catctgataa gatttctttt tgttcactac ttaaggcgcg ataactatct tgtaataatt
     4621 taatattaaa tgattgtggt gtttggcctt gatacatttc attccttaca gggatactgt
     4681 gtatgttctg tttatcttta gacattacaa tcgtatcaat tgcttcaatg actgtatcta
     4741 ctgcaccata ttttgctgct acttcaatgt tctctttaat aatacgttga gttaaaaatg
     4801 gtcttacggc atcatgagtt acaatcacat catcattatt aattccattt acattgcgaa
     4861 tatggtcgat aatgttcata attgtttcat ttcgatccgt accacctgca actactttga
     4921 cacgttgatc tgtaatgtta tattttttta aaatatcctg tgtatgggaa atccactgtg
     4981 ctggcgttgc gataataatc tcattaaatt cactcactaa aatgaacttc tcaattgtat
     5041 ggattaaaat cggtttatta tcaatatcta aaaattgttt tggtaatggc acgttcccca
     5101 ttctcgaacc aatacctcct gctaaaatac ctgcataaat catgttgtcc tccattctgt
     5161 cattacatca tttccattta tacattactg acctatgccc gcacataagc ctaacctatt
     5221 gctcacttgc ctcttttatt aatccaaaga tagttgtcac aatagtgtga taatttttta
     5281 taaaaatgta tttttgtaac tgaccattct aagttgtttt gccatgcagt taatcattaa
     5341 ctctgacgat attaaattgt taaaggtatt aatgtttact ctttttcaaa ttcattatta
     5401 ctgccatcat tttaccatat attataataa atttatctta ttaagtggct gtacttgatt
     5461 ttcactttaa aaattatcaa atattgccat ctcattttaa gtatacaaaa tgcaaaacaa
     5521 ccgattcaca agcatatttc acacaagtaa accggctatt tatcaacgta tattcgaaga
     5581 tgaattattt cgatagtatc tatagaccag acggcattcg cactttcata gctataacta
     5641 taccagcgtt ttcgtcctca aaggtgcata ctaataaatc gtaaacatga ctttatcaaa
     5701 tcgttctttc ttgttaacta atttatcaaa tgtctccggg cctttttcta acggtaaaaa
     5761 atgagaaata ataggcttta cattaatatc tttcgtcttc atataatgta aggttgccgt
     5821 ccactctttg cccggaaaat tactggacaa acagttccaa gagccacata ctgtcaactc
     5881 gttacgcaga attttttcaa aatgaacgcg atcaatctca atatcatcat atggtattcc
     5941 gagtaatacc acctcgccac cttttttagg tagcgtcaat atttgaccaa tcgtaacttt
     6001 agcacctgat gattctatag ctaaatcgat ttgattggcg taatgatttt cgatgaattt
     6061 ctcaagattt tcttcttttg aattgattgt ttgatgtgcg cccaatgatg ttgcaatatc
     6121 tagtttatgc gcatctatat ctatagcgat gatatgtgca gcaccaaata ttcgtgccca
     6181 ttgaatagct aacaaaccta tactgccaca ccccattact gcaacagtca taccaggttg
     6241 tatattcgat ttataaaacc catgcgcaac aacggctgat ggctcaacca ttgctgcttc
     6301 aatgtaatca acattgtctg gaacctttaa aacattttgc gctggcaatt tgacatattc
     6361 cgcgaacgat ccaggttcat atgagccaat gacgaataac ttttcacatc gtgcatattc
     6421 accttttaaa caatactcgc attgataaca aggtattgct gggcaacctg tcactttgtc
     6481 gcccacatta acatgcgtaa catcacttcc aatggcatct actacacctg aaaattcatg
     6541 accaaatggc atacctttaa tgtatggccc catttttttg tatcgtgacg tgtctgaacc
     6601 acatatgcca gtcgctcgta ctttaataat aacgtcattc gcactttcaa tgactggctt
     6661 ttcattatcc tcataccgta aatcttccac gccatataat ttcaatgctt tcacttgtaa
     6721 atcacctcaa atttgattta attcacaact tttttctttt taaaaatacc tgtcgcaaaa
     6781 taacctgcaa tgacaatgga attacttacg agtaaatgtt ccatataaaa atcagtgatt
     6841 tgtcttaatg gcccaagcat aaaagttagc attgattcta ccatattaat tctctccttc
     6901 attttcatct aaatcgagcg gcttaagtaa tactttaata ccttcgccgg attttaaatg
     6961 ttgatacgcc tcgtcccatt tcgaaatatc atatattttt gtcaccaaag cttcagcatt
     7021 tactaaacca tccgccataa gttgcaatga aggttcccaa tctgctggct tttgacttct
     7081 actaccaaca actgttattt ctttttgaat cactttttcc atatcaaatg gaatttcagc
     7141 atccttaaaa atacctattt gactgtagaa accttttttg cgtaaaatat ccaaaccttg
     7201 tcgtgctgct ggaactgcac ctgaacattc aacaacaaca tctgcaccgt aaccgtctgt
     7261 aattccattg atatacgttt ttaagtctgt ttgttgtaaa ttgactacat aatccatgtg
     7321 caatgcttct gctttatcta atctgacttt gtcattgtcc aatccagtta ccacaacagt
     7381 tgcgccttta ctttttaaca cttgtgctac aagtaatccg attggcccag gtcccattac
     7441 aactgctaca tcgcctgaat tgacttgaat cttagaaacg ccatgatatg cacatgctaa
     7501 tggttctgtc atagctgcag actgatacga tacttcgtct ggaatatgat gcaaactttc
     7561 ttcacgtgca atgacataat tagtaaatgc gccatcaact tgtgttccaa taccttttcg
     7621 atggttgcat aaattatagt cttttgattt acagtattca cactcattac aaacatagaa
     7681 tgtcgtttca gatgtgacac ggtcaccaac tttaaaatct ttaacgtctg ctccaacttc
     7741 aacgatttca ccagaaaatt catgacctaa tgtcactgga aaattaactt tataatgacc
     7801 ttcataagta tgaatatctg tgccacaaat tcctgcataa tgtactttaa tctttacttt
     7861 atcatctagc ggtgttgcaa cttctttatc aagaagttct aagttgccat gtccttctct
     7921 tgtttttact aaagctttca ccacaaacac ctcgattttt aattgaatag actaaatagt
     7981 ttaaagataa gatagttaac gatattacca ccttgatcaa tacttgaaat ttcagatgaa
     8041 ccttttggca tttgtacatt cgtacctttc gccatatctg tgaaaatggg tgctacgtct
     8101 gttgcaatat atagtgaaat tgcaatcata atcgtaccca caatgacaga atgaataatg
     8161 tttcctcttg ctgcaccaac aataaacgcg acaacaaatg gtatcgttgc taagtcacca
     8221 aaaggtagta cttggtttcc tggtaaaata acggctaata aaacagtgat aggtactaaa
     8281 attaatgctg tcgaaataac tgctggatga cctaatgcta cagccgcatc caatccaata
     8341 taaatttcac gttcgccaaa acgtttattt agccatgttc ttgcagactc tgaaactggc
     8401 attaaacctt ccattaagat ttttaccatt ctaggcatta ataccattac tgcagccatt
     8461 gacattccta aattaatgat gtctccaggt ttgtaacctg ctaacacacc aatacctaaa
     8521 cctaaaatca agccgacaaa tatagactct ccaaatgcgc caaaacgttt ttgaattgtt
     8581 tcaggatcag catctaactt attcagaccg ggtacttttt gtaacaattt aactaagtaa
     8641 atacctggtg cataagaaat tgtacttcct gtagcaatcg atacacctgg taaatcaaag
     8701 aactcactca tcatcggtgc tgtccaatct gctactttca aacagataat ttggaaaata
     8761 actgctgcta ataacgcttg ccaaatactg cctgatacgg cataaaccat tgctgctgta
     8821 aacgtataat gccaaaaatt ccaaatatct acattcatcg tctttgtcac tttagttact
     8881 agcaatacaa cgttaactat gattccgagt ggaataataa atgctgcgac agatgatgcc
     8941 caagcgatag atgatgttgc tggccaacct acatcaatca cattcagact gacgcctaaa
     9001 tttttaacca tcgcttgtgc tgctggccct aaatttttaa ctaataaatc gatgactaag
     9061 aaaatcccta caaaagccac acctattgtt aaaccagacc taaatgccgc tccaattttc
     9121 tgcctaaaga ataggccaag caagaatatg acaaccggta aaataacagt tgcacctaaa
     9181 tctaaaaatc cccttacaaa atcagtgaag taactcatat ttaaaccctc cctgttatat
     9241 atgcattgtc acgatacttt ccgattgtga ttacatttga cgttacagtc atttcaacga
     9301 caacccttgc taaattcgac tgcagtcctt ttgaattaca gtcactgcgt ttctatgtca
     9361 tcaacaatca tttgtcgtga tagtcattta tatgcaattt gcatatatta atatgttatc
     9421 gacccacgtt acatatcaat tccgttattt ttgtaactct gttaagattt gttgttttgt
     9481 ttcttcaata ccaataccag ttaagaaatt acgtgcgttg ataactggga atttatattc
     9541 tttttttgtc attgcagttg taactaataa atctgcagtg tcttcataag gtccaacttc
     9601 tgtaattttg atttgtttaa tatctacttt aatattgtgt tcctttgcca tttcttcaat
     9661 tgcattattt actactgttg acgttgcaat acctgcacca cacgctacta atacttgttt
     9721 cattttcaat tcctccaatt aatttttagt tatattccaa ataatcattg attagtgttg
     9781 ctaaaattgt ttcatctttc gttcgtagaa tctgctccaa tttttcttca ctttgaaaaa
     9841 tttgcatcaa ctgttgtaac agcttaagtt gatcatctac tttatccatt gctaacataa
     9901 aaacgatttt cacttctgtc tgttgatcaa gtgttcccat ttcaataaac ggcacttctt
     9961 tttctagaac agccacacct atcgttctat ggttaatatg ttcgacatct gtatgcggta
    10021 tagcgaccga acatagatgc gttggtaaac cagtagcaaa ttctttttct ctgtcgatga
    10081 ctgcatcttt aaacgttgac ttcacgaacc cattttgaaa taacacatct gacatttgtg
    10141 acaatacgga ttctttatca gttgccgaca aattgagcat tatattttct ttatgcacta
    10201 attgctgtcc catccatttt ccctcgcttc tttatttgaa taatttttta aaatctcatt
    10261 tacatcagaa tttttgcgac tttgtatgat gcgcttaatt gcgtcattgt cttgcgccac
    10321 atctctcaat tgtagtaacg ctcttaagtg tgtcacttta tcaacagcag caataggtac
    10381 aataatatgg attgctgtgc catctgacat gtatattggt tcttgtaata tcaacatact
    10441 catcgctgtt ttatgtacat gcttttcaga gtatgcatgt ggaatagcaa tattttgcat
    10501 gataaccata tacgaatcat caaatacctg ttgcatctca tgaatatatt taatatcaat
    10561 aaaatgatta gcaactaaca catcacttgc tttagcaata gcttcatcaa tattttcaac
    10621 atgatgcatt cttttcacgt gccttgccgg tatcaagtca gctaaatcta atgtcttatt
    10681 ttgtgtgaca atcgatccat taatggttga aattgaatta taattggcaa taaaatcttc
    10741 taaaccatca cgtagttctg taatgtcatt aactgtcgtt gtgcgttcaa ttaatgccat
    10801 taacttgttt atttccttat caatgtcagc cgattcctta ttaatgtact tcatcacttc
    10861 tttacgtaac tttcgttgct cattttcaga taaagctact tttgtgataa ataatttttt
    10921 atgtgttagg acaaacattg gtgaaaagac gatgtcataa tctaatgtgt aattttcaaa
    10981 tgttctaagt gaaatcgcat ctaagaaaat aatttctgga aataagtttc gcaactcgta
    11041 taacatcatt tgtgatactg acgtgccttg tgtacacacg ataatagctt ttatcttgcc
    11101 atcgaagttt tcatcttgac gtctcaaact acctccgaac aacatggtta aatatgctat
    11161 ttcattatca ggcaacgatt ttccgaaata ttcagttaac gattgacatg attgtttcac
    11221 catatgaaat aaggattgat aatttccttg taaaggattt attaattcat cacgatccgt
    11281 taagttatat ttaatcctat aaaaagcagg cgttaaatgt aacaagagtt gctgtgataa
    11341 tttctcctta tcttcaatgt taataaaagt gatttgttca aaatggtgaa tcatttgagc
    11401 gatggccatc gttaaattcg atatgctatc tgattcttgc aaatcagtcc attgcacact
    11461 tgttgaaagt aagtgtaatg tcaaatataa cttttccgct tctggcaaat ccggctcatg
    11521 ttgcgtcata atctccgttg cttgatattc tttcgtatcc ctcaaatact gataattaat
    11581 atttaatgga ttcatcacat gaccactttg aattcgtcta cgaatcacac aaaggacata
    11641 aggcaatgaa ctaagtgatt tgtctataaa gcgactcttc aaaaattgtt ctacctgttt
    11701 gatcttgtct ttttgatatg cgatatcttc gaatgttaag ttgagcgcct ttaaaacttc
    11761 acttttagta atatcatgat tcaacctttg atcaatcaac ttaatgaaga aacggcgaac
    11821 ttcaaattca tcaccaacaa tttcataacc atgttttcga gaatacttaa gtgacaaacc
    11881 atgattttcc aattgctctt tcacatgatt tatatcgtga atgacagtat ttttactgac
    11941 ttgtaaatca attgaaaaat ggtttagaga cattgcgttt tccttactaa aaagcatgag
    12001 cattaaataa taacgacgtg tttctatgct aaaaatgaca ttgttgccgt ttaacatttg
    12061 ctgctccgat acatctcgct tgaataacgt catgatttca gaacttacaa taaaatttcc
    12121 ttggcttgtt ctttcaagtt ttggataacc ctcttgttca agccacaaat tgattttttg
    12181 aatgcgatat cctagttgtc tacgagacaa accaaatatc gattcaagtt ctttaccatg
    12241 aatagtagga ttcaatacaa tttctctgag tatttgctga ttatccccgt tcatgttcga
    12301 ttctcctttg agcatctgag ctcaatttat catcaatcgt cacaccgatg tacacacttt
    12361 gaacacatat tttcaaaatg agcatgtaca tcattgtgat gttttaacaa catttcaatt
    12421 atatctatat tttttgtgat tttaatcttt taaaataaag caattgaaat ttttgcatat
    12481 atttttgtgt tttgtgtttt tttgaagcat ttttaacata catatctcaa tcattatcaa
    12541 attgtcatga ccattgtaac ccaatacaaa aaccctaagg acgcttatat caggcgcctt
    12601 agggttaact gtatctattt aattaagtat tattattcgt atgtacgtaa cttatggtct
    12661 atcaagttcc acacttcttc aacatcaact gctgtagcaa aataagcatt ggcaggctta
    12721 cctgtaacat gatttaaatc gacagccata gtgccataag ttagtggact ttgatgttca
    12781 atgtcgatat taacgggtac cattgtaaac aattctggtt gtaacaaata caaaattgta
    12841 caagcatcat gtattggacc accatccata ttaaagtgag tcttgtatgt cttcttaaag
    12901 aattgcaata attctacgac gaactgtgca acaggattat tgatactttc aaagcgttca
    12961 atcacgtgat cgtcggctaa aacttgatgt gttacatcta aaccaaacac atttatagta
    13021 atcccacttt caaaaacacg cttcgctgct tcagcatcta cccaaatatt gaattctgct
    13081 gtaggcgtcc aatttccaaa tgtaccacca cccatcaaag taatagattc aatatgctca
    13141 gcgattcttg gctcacgaat caatgccgtt gctacattcg taagaggacc tgtcgctaca
    13201 attgttacag gtgtatcact cgtcatcact ttgtttataa tcacatctga tgctggcatt
    13261 gcaactgctt gacgtgatgg tgtcgacggt agtttcggac catctaatcc agattcccca
    13321 tgtatttcag aagcaaaggc agctggttta attaacggcc tatccgcacc tttcgctact
    13381 gctatatctt ggcgtcccat aatatccaat acgttcaagg cgtttgtcgt attcttgtca
    13441 actgattgat tacctgcgac tgttgttaca gctaatatct ctagtggact gtcaattgcc
    13501 cccgctaaaa ttaatgctat tgcatcatcg tgtcctggat cacaatccat aataatcttt
    13561 cttttcattt atatatccac ctttcttaag ttgttatcga tagcttatgt atatttattt
    13621 atgtggtgaa tcatgtttat tttgaaaaat agttttaact ttctcatatt tttggataca
    13681 aacactattt atctatttta tggcttataa atttatccga tatgccttat caacctacct
    13741 cgctaaaaat aggatgtcta catatctata ccgacttttg tcaactcatt ttcacaacaa
    13801 tataaacagc aatttatatg attgttacat gattcaaaca atttttatga aaaatatttt
    13861 catacacaga atatatattg atattaaatt tctcaaaagc tatattgaga ataattagga
    13921 gggatgttga tgaaatcttt atttgaaaaa gcacagcagt tcggcaagtc ctttatgtta
    13981 cctatcgcaa tcttaccagc tgcaggtcta ttgttgggta tcggtggtgc attaagtaat
    14041 ccaaacaccg ttaaagcata ccctatttta gatattacct tattacaaaa tatttttaca
    14101 ttaatgtcag ctgcaggtag tattgttttc caaaatttac cggtcatctt tgcaattggt
    14161 gtcgcaatcg gattatctag aagcgataaa ggtactgcag gtttagctgc gctgctcggt
    14221 ttcttaatta tgaacgcaac tatgaatggc ttattaacta tcacgggcac attggcaaaa
    14281 gatcagcttg cacaaaatgg acaaggcatg gtgctcggta tacaaacggt tgaaaccggt
    14341 gtttttggcg ggattatcac aggtattatg accgcaatac ttcacaacaa atatcacaaa
    14401 gtggtattac caccgtattt aggtttcttt ggtggctcta gatttgtccc tattgtcaca
    14461 gcatttgccg caatcttttt aggtgtattg atgtttttca tttggccaag catacaagcc
    14521 ggcatttatc atgttggtgg atttgtaacg aaaacaggtg ccatcggtac ttttgtttat
    14581 ggcttcatct taagattgtt aggtccactc ggtttacacc atatttttta cttaccgttt
    14641 tggcagacgg cacttggtgg tactttagaa gtcaaagggc acttagttca aggtacgcag
    14701 aacatcttct ttgctcaact tggtgatcca gatgtgacga agtattattc aggtgtgtca
    14761 cgctttatgt caggccgttt tattacgatg atgttcggct tatgtggtgc cgcacttgca
    14821 atttatcaca cagctaaacc tgaacataaa aaagttgtcg gcggtttaat gttatccgct
    14881 gcactcactt catttttaac aggtattacc gaacctttag agtttagttt cttgtttgtc
    14941 gcacctattc tttatgtaat ccatgccttc tttgatggat tagcatttat gatggcagac
    15001 attttcaaca ttacaattgg tcaaaccttc agtggaggct ttatcgattt cttactcttt
    15061 ggtgtgctac aaggtaatag taaaacaaac tacctatacg tcatacctat tggaattgtg
    15121 tggttctgtt tgtattacat cgttttcaga ttcttaatta cgaaatttaa tttcaaaaca
    15181 cctggtcgag aagataaagc tgcagcacaa caagttgagg ctactgaaag agcacaaact
    15241 attgttgctg gtttgggagg caaagataac attgaaatcg ttgactgttg tgcaacgaga
    15301 ctacgcgtca cacttcatca aaatgacaaa gtcgataaag tattactcga aagtactggt
    15361 gccaaaggtg taatccagca aggcactggt gtgcaagtaa tttatgggcc tcacgttaca
    15421 gttatcaaaa atgaaattga agaattgctc ggggattaag actaaccgaa atatcaacag
    15481 aactaatggc aacgatgtac gaagtaagaa gtgacatcgt tgcttttatt tttaatgtta
    15541 catttgaagc attaagttca tcatgcactg tagtgagccc gcaaatcgcc tctgctagac
    15601 aatcatctta atgctatgat taaagcttaa gtgccagatt tgaatttaat ttcaacaacg
    15661 actttcacta cattaaaaat agggctactc gacacatata gttgtatcaa atagcccttt
    15721 atacaatttt ttgggtaagg ttttacaatt tttgggatgg tatagatttt ataaaaagtt
    15781 atttaagttc ttctgcttca gccataatat cttttaatgt tttagctgaa tgtgcgaact
    15841 tgctttgttc ttcgtcgttt aatgggattt ctacgacgtt gcgaataccg tttctattga
    15901 tgactgctgg aacaccaata taaacatctt cttcctcata ttcgccttct aataatgctg
    15961 atacagtcaa tacggcatct tcatttctga aaatcgcttc agtaattcta gctaatccca
    16021 ttgcaacacc ataataagtg gcacctttag cttgaataat gtcatatgct gcatcacgtg
    16081 tttgaacaaa aatttgttca atttgcgctt tgccctcagg acgttgttca agtaatgtct
    16141 tcaaaggttg acccgcaata ttagcgtgtg accatactgg taattcagtg tcaccatgtt
    16201 caccaataat ttgagcatcg acgctacgtg gcgcaacatc gaacgcttcg cttaacaata
    16261 atctaaagcg tgcagagtct aaaattgtac cagaacctat aacacgttct ttaggtaaac
    16321 cagagaattt ccatgttgca tacgctaaaa tatcaacagg atttgtagct accaagaaaa
    16381 taccatcaaa ttttgatgcc attacttcac caacaattga tttgaatatt ttcaagtttt
    16441 tagatactaa atctaaacgt gtttctccag gtttttgtgc agcaccagca cagatgacaa
    16501 ctagatccgc atcatgacaa tcactgtatt cgccagcttt cacacgaact gttgttggag
    16561 aatatggtgt ggcatgtttt aaatccataa catctcctcg aactttttca gtgtctaaat
    16621 caatgatgac taattcatca acaatgcttt ggttcactaa tgaaaatgcg tagcttgaac
    16681 ctactgcacc attacctatt aatacaactt tgttcccttt aaatttgttc attacaaaaa
    16741 ctcccttatg attaattcac taacatacat gtagcttcaa atatgttagt ttaatgctgc
    16801 ttattgacga tacaaaagca aataaacatc tcttttattt tcaacgcata acttaaaagg
    16861 tcatgtgtca tccgctttta agtttgtgat ttatttcaca tataaaatgt aacatgcatt
    16921 aagtactggg tcaatattaa attgtgattt atttcacatt ttattttaat ttttacacct
    16981 ttttaatttg tatacgatta catcttagat gtctttagtc ttcgtacttc gccagtgatt
    17041 atttacactt tcacattttt attatcatgt ttactttttt ctaggaaaac aacaatgttt
    17101 tttgaattag tcaaataaat gcgctcaatc gtcggtgtgc aaacagacaa ttgtacacaa
    17161 tgcttattga taagtattta aaaaattaaa aatgtcatac aattatcaaa tttgccattt
    17221 tatttatatt ttctcaaacc aattaattga atatcgaaat ttttagtaga ataatcaaaa
    17281 tatacagatt aaaggaggag tatcatgctt acagaacaag agaaagacat tatcaaacaa
    17341 acggtgcctt tacttaaaga gaaagggaca gaaattacgt caatctttta tccaaaaatg
    17401 tttaaagcgc atcctgaact tttaaacatg tttaatcaaa cgaaccaaaa acgaggcatg
    17461 caatcttcag cattagcaca agctgtaatg gccgcagcgg ttaatatcga taacttaagt
    17521 gttattaaac cagtcattat gccagtcgca tataaacact gcgcactaca agtttatgct
    17581 gaacattatc caattgtggg gaaaaattta ttaaaagcca ttcaagacgt gacaggatta
    17641 gaagaaaatg accctgtcat tcaagcttgg gcaaaagcat atggcgttat tgcggatgtg
    17701 ttcatccaaa ttgaaaaaga aatttatgat caaatgatgt ggattggttt taaaccattc
    17761 aaaattacca atattaaaca agaatctgaa gacattaaat catttacagt tgaaactgaa
    17821 gaatatgact ttagtgaatt tacaccaggc caatacatca cagttgatgt ttctagtgat
    17881 aaacttccat atagagctaa acgtcactat tctatcgtat caggtgaaaa aaaccattta
    17941 acttttggcg ttaaacgtga tgtcacaaca gaacatgaag gcgaagtttc aacaatttta
    18001 cacgatgaga ttaaagaagg cgatatgatt aatttagctg cgcctgtagg tggattcgta
    18061 ttagagaata cgactgaacc acaacttttc ttaggttcag gtattggtgt tacaccttta
    18121 gtagctatgt atgaagctgc ctctgccaaa ggtttagata cacagatggt tcaagttgct
    18181 gaaaatgaac aacatttacc tttcaaagac aacttcaaca gtatcgcaag ccatcatgac
    18241 aacgctaaat tatatacaca cttaaaagat aaacaaggct atattggtgc tgaagaatta
    18301 caagtatttt tagcaaataa acctgaaatt tatatctgtg gtggtacaaa attcttacaa
    18361 tctatgatcg aagcacttaa atctttaaat tacgatatgg atcgcgtaca ctacgaaaca
    18421 tttattccaa gactaagcgt tgcagtataa ctgagaaaag aggtttagtg ctcatatgaa
    18481 acttaatcaa cgttacgtaa aagtatttgc attatatttc gtaagtattg ttactgcaaa
    18541 tattattgtt aaaaataata atttaattaa aacattgata caaaccatag ccgggtacac
    18601 ggtctttgca gttggtttga agtatttaac taaacgtaaa aataaatgac atctatacca
    18661 aaacagctat gactttactt tgtcatggct gttttttaat tgggagtagg acagagatga
    18721 tattttcgca aaatttattt ggtcgtccca ccacaacatg cattgatgta tgctcactga
    18781 atttcataag aaaggagttc acaagatgac cgtagatatt ggacggattt atgacaataa
    18841 agataatacc gacgctattc gtatcctagt cgatagagtc tggccgagag gtatttcgaa
    18901 aagaactgct aacctagatt attggttaaa agacattgcc ccttctactg agttgcgaca
    18961 atggttccaa catgatccta aactttttgg agcttttaaa gaaaaatatg aaaaagaatt
    19021 acgtgatcag gatgcgcaaa aagatgcttt tgaaaaatta aaggatattg taaatcagca
    19081 taatcatgtt ctattgttat atgcagcaaa agatactaaa cataaccaag ctgtagtact
    19141 acagcagttg ctcaatactt agttatatat gtttatcgtg aatcactata aatgtcgcta
    19201 acttcatttc taatatttat ttttaaaaag cataattact acaattaatt gaactttaat
    19261 aattactaaa cttgaacaac attttacttt aacaaaataa agttttaaaa tattaattgt
    19321 tgggttttac atagtaatta gaaagagagt gttaatgaaa tgaagtttaa aagactagca
    19381 actatatttt cagcagtatt agtgttatca ggatgtggct caatgcattc atctggcaaa
    19441 gacttaaata tttcgttacc gttaaaaaca aagtctattg caccttatga aacagatgta
    19501 ccagttaaga ttggtgctgc agaatcatta tttaaaacaa atgatcaagg aaaaatagaa
    19561 aaagcattag tgaaatcgta tcatcaacca aatgatacaa cgttagatat agaattaaaa
    19621 gataatatta aatttcaaaa cggtcaaaaa ttgactgcag aaaaagtgaa atctagcctt
    19681 gaaaatagca tgaaaaaaag cgacttggtc aaatattcat taccaatatc atcaattacc
    19741 gctaaaggtc aaaaactgac aattaaaacc aactccgctt accctgaact tgtatctgaa
    19801 ttagctaatc cttttatggc aatttatgat acagatgcta aatcagatgt taatcaaact
    19861 cctgttggta caggccctta ccaaataaaa gattataagc aatctcgaaa aatatcattg
    19921 tcgaatttta aggactattg gcaaggtaaa ccgaaacttg atcatattac tgtgacgtac
    19981 caagaagacg gcaataatcg cgtcagaaat ttagaatctc aaaaagatga tttaataact
    20041 gatgtcccag ttaataaagt tcaagacata gaaaataatc aaaatttaaa agtgtcaaaa
    20101 gaatctggat ttagaacttc tttacttatg tataatcata ctaataaaaa aatgactaaa
    20161 tccgttcgtg aagcattaga tcatatcatt gatagacaag gtattgcaga tcatatttat
    20221 caaggttatg cgaaacctgc aacgagtcca tttaatgata aaattccata tattaaagaa
    20281 cctaagttaa ccaaacaaaa tatcgaacaa gcaaaaatgt tattagctaa agatggttat
    20341 acaaaagaac accctttaaa aattaagtta attacgtatg acggtcgtcc agagctatcg
    20401 aaaattgcgc aagtattaca atcagatgct aaaaaagcaa atattgaaat cgacattaaa
    20461 agtgttgatg acatagaagg ttacttaaaa gaccgttctg catgggatgc aacgatgtat
    20521 agtttcggaa caattcctcg tggagataca ggttatttct ttaatcaagc atataaaaaa
    20581 gatggtgcta ttaataaagg agactacaat aatagcaacg tcgatgattt aattaatcaa
    20641 ttgaatcata ctgtagatgt taaggaacgt cacaatattt ctaatgatat tattaaatta
    20701 tcaagtcgag atgtaccaaa cagttatatt gcttacaacg atcaaatagt cgctgctaac
    20761 tctaaggtta aaaattataa agtaacacct gaaggtatct atttaatcga ttatcgaaca
    20821 acgattgaac gataagggag tgtgggacat tgatttatgt ctcagccctg cttcaactat
    20881 aaaaaccacg ccgaagtcat ccaatatgat tatggcgtgg tttaatttgc ttatatgatt
    20941 gtattaattt ctgtttgatg aatttcaata aaatgattat tacatacttt cagtgaatca
    21001 tgttcatttt atataaatct aattgtcact atttgtttaa cgtgaaacac ttttttaact
    21061 ccaaaaagta ttcctattcc actcttttaa agacaaatta taaaaatagc ttatgtcaca
    21121 aacatatgag caacataagc tttaattatt attcgtctac ttttttctgt tgttctttaa
    21181 attctttctg cagtaaattc acttgtttta aacctgcccc cattaaaatg aatacaagta
    21241 tccacacaat aacgattaaa ataaatattt tcaacgtatc actgccaagt acagttaaag
    21301 atgtatccca aatgccatgt aaaacaacgg ctgataaaaa gaatattaaa aagcgtttat
    21361 caaaaatatc tttgaattca aagccatgct gttctttcgc aataactatt gcagcaccaa
    21421 caatcgctga ccaaactaaa tgaccaccaa tcgcagtcca cgcacgtgtg aagacaatat
    21481 ctaataatgg gacattttct cctaaagcga aattcaaaat ataacctgct gattcaaaaa
    21541 ctgcgaaccc tgcaccaata gcagcaccga ttaataatcc attcaaaatc ttatttgttt
    21601 tcaatttatt gacgaaataa acaataatga gtgcttttcc agtttcttca actaatccta
    21661 ctaaaaatgc atcgaaaaat gttaaagaac caaacctttc gaattgatca ctaaaaacga
    21721 caaatctata taataccatc gtacttagta atgaaaatac gccgccaata aagaacatga
    21781 taataacttc aaaaatgcta atatttttaa acgcatttga ttcataaaag aagaacaaac
    21841 ccgataatgg tactgttaaa gcccctataa aaatgagacc cggaatcgcg ttagtgttat
    21901 taaaaattgc tgccatgacc cataatccaa taaatgttac tgtgaaagcg atgaatactc
    21961 gagaaaagag ccatggcttc ccccattctt ctgatatgtc acaaattgct ggcgtagttt
    22021 tagcagtacc cgctataaat atttcatctg cttcgttttt agtatgcggc ttaaatactt
    22081 cagaaagcat atcttttaaa ttaagattta agctttcatc attgcccgct agtctaccga
    22141 ttgatttggt tgcattatca aataaacttc ttgcttcgtt ttttaaatct gtattttgcg
    22201 ctgtttcatg cgctttagct tccatttgtt tatcgataga ttcattatgt atgtcggacg
    22261 tcttagttga ttgtgtactt gctgatgaca tcccctcttc acctgtatta ggtgcaactg
    22321 attgcgtttg tgttgtctct tctttttctg tatttcttcg atgattaaga ccttccatag
    22381 acttacctac tttcactttt cataataaaa ctattttaat ttaatattga tggattttcc
    22441 atattttaat ataaatactt tgttcgattg ggcactttgt atttcatatt taaaaatcaa
    22501 aacctattta actttttata ttccgaacaa tatttttata taagtatttt aatgatattc
    22561 aattcattac aaacccttga tattacaatt gttatcgacc gtcaaaatgt gtctatactg
    22621 tgtctattat cagaataaaa agatatttct aattgatttt aaacgtcgtt atgttatatt
    22681 tcttgtaagg gtttacacaa agtgtaaaaa cgctatattt tatcaaatgt gatggaggtg
    22741 acttgcttga aacaaatcac atggcacgac ttacaacata tcattaaaga tggtgatgtg
    22801 attggtttac cagcattagc tgtagccaac ttacccgccg aagttctacg tgctgtgtta
    22861 gcgcaacatg acacatatca tacgcccaat gatttaacgt ttatattagc gaatgatatt
    22921 catagtttag gtgccgcacc ggatttagat gattttatag aacgtcgcat gattaaacgt
    22981 gtcattatga gtattttaac ggcttcttcc aaaacggcac aagcaatgaa aaataatgac
    23041 attgaagctt attttttacc acaaggtatc attgcaactc attatcgtca gagtaatcaa
    23101 ttattacctg gagttattac taaaatcgga ttaaacacag ctgttgatcc tagatacggt
    23161 ggcggtaaag taaatacacg aacaacggat gatttagttt cattagtaac catcaacgat
    23221 gaaacatact tacattacac attccctagc gttgatgtgg cactactgag aggaacatac
    23281 gcagatcaac aaggtaacat ttatttaact caagaagcgt acttgagcga gtgttatcat
    23341 gtcgcattaa acgcgaaagc caatcatggg aaagttattg tacaagttaa agctttagtt
    23401 gacgactatc aactaaaacc gaatgaagtt gttatcccag gaaatcttgt cgattatgta
    23461 tacgtcacag aagatgaaaa gaatcaccgc caagtaattc aaagtcatta tttaccagcc
    23521 ttgtctggag aagaacgaat tgatggaata cctgaacccg cattaccttt taatagtcgc
    23581 aaattgattc tccgacgtgc tgctcagttt ttaacttatg gcgatacaat tagcatcggt
    23641 tatggcatca ataatgaact ctctaattta ttgcatgaag aatgtgttga acatgatgtg
    23701 caaccgattt tagatgttgg cattttcggt ggattcgttg ggagtcgtga acattttggt
    23761 atgaattaca atgcagatgt gcgcatgcct catgatcgag catgggattt tatttataac
    23821 aatggtgtat cagttgccta tcttagcttt gctgaggttg atcaatacgg caatgtcaac
    23881 gtgtcttact tcaatgaccg actaaatgga tgtggtggct ttatagacat tacgcaatct
    23941 gtaaataaaa ttatcttttc aggtactttt gtagctggca gtcatgtctc atgccataat
    24001 caacgattaa acattgaaac tgaaggacaa aaccagaaat ttgtatcaga tgtgagccat
    24061 atcgacttta atgcacaata ttcacaatca ctcgagcaag aaatctattt tgttactgat
    24121 cgtgcagtat tcgaactcac caatcaaggc ttgaaactaa ttgaaattgc accaggtctt
    24181 gatttgcata aagatatttt gaatcaaatg gcttttaaac caattattgc tgatcattta
    24241 aaattaattg ataccagcat ttacaaagaa aaatggggac aacttaaaca atcaattcat
    24301 aaagtatgaa agtgaagcta aagggggtat actaatgaat ttcgattgga taaaaacgcg
    24361 ttcagacttc gatgatgaca agcctgccgt tattgatcac gcaaaacaaa catcttggac
    24421 ataccaacaa ctcaatgcac gcgctgataa tatggcacat tatttaacat ctcaaggtgt
    24481 taaaaaaggc gatgttatcg gtatttttgc gccaaatgat attgcaatat tagatttatt
    24541 gtttgcttgt tttaaaacag gtgcggtttt tttaccattg aattggcggc ttaacccaaa
    24601 agaaattgca gccattgtgg aagatgcaca attaaaactg ctcttctatg ctgaaaaaca
    24661 tttaagttca ctcaccgata ttgaccaaaa cttattgcat atggatattg atgtggcgca
    24721 atatgatgaa atcgtaaatc cagattatca ccaacctttt caagcaacac ctgttgaacc
    24781 acaagatctt gcagcattaa tttatacaag tggtacaacc ggatcaccta aaggtgtgat
    24841 gttctcatat gaatcatttg ttcataacgg tgcaaactta gagctgacgt ataagttcaa
    24901 ttcaaactat attacgattg tatcaacacc aatgttccat gttttagggt ttaacgatac
    24961 tgtattacca gtattaatgt caggtggcac acttattctt caacgttact ttaacggcga
    25021 agaattgaat gacatgattg cacaatatca ccctacattt attatcatga ttccgacaat
    25081 gtattatagt acgctacgtg ctagtaattt taatcctgaa aattttagag ctatggatta
    25141 tatcatccaa ggtggttcac aaccattacc aagtatccaa gcagctttta aacaatatgg
    25201 cataaacatt attaacggct atggtttaac tgaagcacct cttgtacttg ttaatacacc
    25261 ggaaaattca aaacgtaagc caatgagtat tggtaaagca gtcatgttcg ttgatgcacg
    25321 tatccttgat gataacggtg aggaagtacc tactggtgag attggcgaac ttgcaattaa
    25381 ggctaaaaat gtcacgccag gatattggaa taaaccagca gagactgcca aagcatttca
    25441 tggtcgatat ttattaactg gtgacttagc gaagatggac aacgatggcg atatatttat
    25501 tattgaccgc aaaaaagaat taatcataac tggtggcgaa aatgtcttac catccgaagt
    25561 cgaaaatgct ttagctgagc atccactagt agaccggtgt gtggtcgttg gctatgatca
    25621 tccaaaatat ggtgaatcaa ttgctgcagc cattatactt cgcgaagatg aacctcatta
    25681 cgctgaaatt ttaaatcaac atatgcgaag tcgtttagca ggttataaag tcccaagaat
    25741 gtatgtacca gtgacacata tgccgttaaa cagtacgcag aaaccagata aacttgcgat
    25801 tcgacaaatg atgaatgaca aagtctcgca aacactttaa aggtgataaa aatttttgac
    25861 atttagtgta agcgtttaca aataaagcgt gttgtttttg aattaaatgc atttcacatt
    25921 agtattcata ttatttttag gaggaattta tatgacattt gaaaaagaaa cggtcttaaa
    25981 aacattattt cctgaagatg tacttagtat tgctaaaggt ttaacagacg gtgaagtcga
    26041 atttttacaa caagtagatt cattgctaga aagtaagtac cgtgaaaata ttaatcaaca
    26101 ttggatagac gctactgtac ccgaggacta ttttaaagat ctgggagaat taaattattt
    26161 taacaatcca ttactttaca aggatcgtcc aaacgccaaa atgcctagtc aactatttca
    26221 gtttttcatg tcttacctac tcgcgcgatt tgatatttcc ttagctaccc tactcggtgt
    26281 tcaccaaggt ttagggcata acactttcta tttcggaggt agcaaagaac aaattgcgaa
    26341 atatgtacct aaattacagt cacatgaact gcgtacatgc tttgctttaa ctgaaccaga
    26401 acacggttcg gacgttgcgg gaggtcttga aacagtcgct gaacgccaag gcgatacttg
    26461 ggttatcaat ggtgaaaaga aatggattgg tggtgcacat gtatctgatg tcattccagt
    26521 attcgcagta aataaagaaa ctggcaaacc ccattgcttt gtagtcagac cagaacaaga
    26581 tggcgtcgat attgaagtca ttgataataa aatcgcactt cgcattgttc ctaacgccct
    26641 aattaaatta actaatgtca aagtagatga agcggatcgc ttacaaaaca taacaagctt
    26701 taaagatatt gccaaaattc tttattcaac gagagcaggc gttgcttata tggctacagg
    26761 tggtatggct ggcgctttac gtgccacatt agattatgtc actgagcgta agcaattcgg
    26821 caaaccaatt agtaaatatc agttaataca agaaaagcta gcaatgatgc aaggtaattt
    26881 agctcaagca atggcaacat gtgctcaatt agctaatatg caagcacatg gtgaatatga
    26941 cgaggttgca acttcaacgg cgaagatgat gaatgcctta cgtttgcgtg agacagtagc
    27001 tatgggccgc ggtattacag gtggtaatgg catactagct gacgattatg atattgcacg
    27061 tttcttctct gatgcagaag cgatttacac gtacgaaggt acacatgaaa ttaatgcctt
    27121 agtaattgga cgcgctttga ctggagattc tgctttcgta taaatagcaa ataattatat
    27181 gagatgcatt aatttcacta aaaaagactt attttaagca taaagctttt tccttaaata
    27241 agaggctaag atgactgtca aagatactta attaatttta taaaatagca acgttattcc
    27301 aattatctta atggttatct tatcctcaac taaattggag gaatcactat gacaattaat
    27361 aaagtaaccg ttcttggcgc aggcacaatg ggcgctcaac tggcagcact ttttgtgaat
    27421 gctggactta aagtaaaact attagatatt gtagtggaca aaaacgatcc aaatctcatt
    27481 gcgaaaaaat cttacgataa aattacagat aagaaacggc cgctactatt cgacttaaat
    27541 ctagcgagtc atttaacata tggtaatttt gatgatgact tggtaaatga tgatgctgat
    27601 ttatatatcg aagcagtcaa agaagatatt gaaattaagc atgctgtttg gcaacaagtt
    27661 ctacaacatg ctaaagaaga tgctttattc gctacaaata catcaggtat tccaattaat
    27721 gcgattgctc aagcatttaa cgagaaggat caagaacgat tctttggtct acatttcttt
    27781 aacccaccac gtattatgaa attagtggag ttaataccta cgtcacacac gaaggaatct
    27841 attatattag atgtaaaaaa tttcgcgcaa aatgtgttag gtaaaggtgt cattgtcgtc
    27901 aatgatgtgc ctggctttgt cgcaaataga gtcggcacgc aaacaatgaa tgatattatg
    27961 tatcgcgccg agcaacacaa gataagcatt gtagatgtgg atgctttaac tgggcaagcg
    28021 attggtcgtc ctaaaacagg tacatatgcg ctatctgacc tagtcggttt agatattgca
    28081 gtgtctgtaa ttaaaggcat gcaacaagta cctgaagaaa caccttattt tcatgatgtc
    28141 aaaattgtaa atacgttgtt tgacaatggc gcactcggac gtaaaacgaa acaaggattt
    28201 tacaaaaagg ataaagaaac taaagctcga cttgtttacg atgttgaaaa acaagattat
    28261 gtacctgtat cgcaaccaca attaccaatt ttaaatgaat ttaataaaga cttagtgcat
    28321 aaccttgata ccatattcaa tgcgcaagac gaagcgggac tatttttatg ggagacatta
    28381 cgtaataatt tctattactc tgctatcaat gtacctaaag ctaccgatga tttccgagac
    28441 atagaccgtg cgcttgtctg ggggttcaac tggaaacttg gtccattcca attatgggat
    28501 gcaatgggat acgaacgtgt taaaacacgt atggaagacg aacttggaga cttaccacaa
    28561 tggattagtg atttagatgg tggcttttat aaacaagatg agaccattga atatgcaaca
    28621 cctatttctc acttcgtaaa agatgaactt tgggataaag gtgatgccaa actttccgta
    28681 actcatgatg atcaactgtt actgaaatta caaagtaaaa ataatgtcat taccgatgaa
    28741 ttcaacgatg cgttagttga tgcgattgat ttactggaaa atgaccatta cacaagtatg
    28801 gttatttatg cagatggtaa caatttcagt gtgggtgcta accttttctt aatgaaaaag
    28861 gcgcatgaag acggtcttgt agatgatgtc gttgcacaat caattgataa attacattat
    28921 agctttaatc gtttgaagta tagtttgaaa ccagtagtca cagctgttca aggtcgtgcc
    28981 ttaggcggtg gctgtgagct tgtactttac tcacctattg ttgtcgctgc aagtgaaaca
    29041 tatatcggtc ttgttgaagc aggtgttggc ttattaccga gtggcggtgg ccttgcagaa
    29101 atggctgatc gcatattacg cacatcgcat aagtttgatg acaaacaagc ttccatgaca
    29161 aaagtactga cgaatatcgc atttgcgaaa gtctctacaa atgcctttga ggcacgtcgt
    29221 tatggttatt tacgtgatac agatacgatt attttcaata cagcacaacg tgtcgaagtt
    29281 gcgctcaaac gtgcgaaata tgaagcagaa acaaactata ttccgaatcc tagacatcaa
    29341 tatatcgctt taggtgaaga cttcaaagca ttgatccaag gacaattaga tgcgcaaaga
    29401 cggggtcatt ttattagcga ccatgattat catattgcct taaatatcgc cacaatttta
    29461 gcgggtggtg atttaccaag aaatacattt atcaatcaac gttacattca atcgttggag
    29521 aaaattggct ttattgactt actaaaatct aaaaaatcat atgaaagaat tgcacatatg
    29581 ttaaaaactg gtaagccatt acgtaattaa aagatagtca ttaagagagg atgataacca
    29641 tgcaagaagc atacattgta gcttatgggc gttcagccgc agcgaaagca aagcaaggcg
    29701 cattattcca cgaaagacct gatgatgtcg cagccaaagt attacaaggc gtattgaaac
    29761 gtattgacgg aaaattcaat aagaatatga ttgaagatgt cattgttggt acggcttttc
    29821 cagaaggatt acaaggccaa aacattgcac gaacgattgc attgcgtgcg ggattatctg
    29881 acacggtacc gggtcaaaca gtgaatcgct actgctcatc aggattacaa accatcgcga
    29941 ttgcagccaa tcaaattatg gctggtcaag gagatatact tgtagctggt ggcgttgaat
    30001 tgatgagtgc cgtaccaatg ggtggcaacg agcccacaaa caatccaacc ttacaatatg
    30061 atgatatagg tgcgtcatat cctatgggtt taactgctga aaatgtagca tcccaatttg
    30121 acgtatcacg tgaagatcaa gatgcttatg ctgtcagaag tcatcaacgt gcctatgacg
    30181 cacaacgtga tggtcggttc aaagatgaaa ttattccaat acaagtaaac tcagttgaat
    30241 atacaaacgc aggaccaaaa gtacacacaa atatctttga ccaagatgaa tttatacgcc
    30301 ctgacaccac gatggaggca ttagccaaat tacgtacagt atttaaagct gacggcactg
    30361 tgactgcagg aacatctgcc ccactttctg atggtgcagg atttgtagtt ttaatgtctg
    30421 gagataaagt gaaagaactc ggcgtgacac ctattgcacg attcgttggt tttaaggcag
    30481 taggcgttga cccgaaaatt atgggtattg ggcctgcata tgcgattcct gaagtattgt
    30541 cactcagcaa tctatctgtt gaagacattg atttgatcga attgaacgaa gcatttgctt
    30601 ctcaaacgat tgcatctatt aaagaagtag gtctagatat atcacgtacg aatgtgaatg
    30661 gtggcgctat tgctttaggt catccattag gtgctacagg cgcaatgtta accgcgcgtt
    30721 tacttaatga aatgggtaga cgtcccgata gccgttacgg catggttacg atgtgtattg
    30781 gtgtcggcat gggtgcagct gctatatttg aatatgtgcg ttagaatggt tgattttgga
    30841 tgaagcggat tcgttttgtt attgaatgaa gtaggctgaa gttgaagcca gttgaagttg
    30901 aagcgggttg aagcaatttc gttttattaa tgaagctgtg tgaaatatag tgattgaaca
    30961 aaaaagtggt ttaatgggat ggtggttatt tccgttttag aatttaacat ttacacgtct
    31021 aattttaatc attgttttaa attttatgaa tcgaagccct ttgatttaat aatatttgct
    31081 aatgctagta acttatctga ttgttcatgt ttaaaataaa gaaaaccact cacatcagtg
    31141 tgtgttcgaa ctagacttgt aagttccagt tcggcacgac tttctaaagc aattattatt
    31201 gctgtgattg tcgtatatca cttagatgtg cgtggtttat tttaataggt tagtaatata
    31261 ttaggtcatg ttatgtttaa gactataatg aataaataat ttagaaatat gcttccgatt
    31321 gttcgatgct ttaattcagt tagaagcatc atagaatgca tgattactgt tgtaaagata
    31381 cgtaatgttt tgtattgact gtatgtcttt ggatagagtt acaaacttat tttgttactc
    31441 taggcccata tgtcgcagta ccatctgcat gtgttgttac attgtatgca tttgttttac
    31501 ttggcttctt gtatgtcggg cgagctccgt atgacacttg accgtttgca tgtgttgtta
    31561 cgttgtatgc atttgttttg cttggcttgt tttgtgttgg gcgagcgcca tatgatactt
    31621 ggccgtttcc atgtgttgtt acgttatatg cgtttgtttt gcttggcttg ttttgtgtcg
    31681 gacgagctcc gtatgatact tgaccatttg catgtgttgt tacgttatat gcatttgttt
    31741 ctgatggctt attgaatctt ggtctcgctt catatccaaa tgttccatcg ttgtattcac
    31801 ggatacctgt accagcatct ctatatttaa catatttagg tgttttgtta aattgcggtc
    31861 tcggaccata ttgagaagct tctgttgttt cagttgcttg aggtttaact tcaatatcac
    31921 ttgattctcc ttgagtacct tttaacgttg attcagtacc ttgtggtttt atttcaagtt
    31981 tagatgagct accttcaaga ccttctaaaa tagggttcgt taacggtggg tttgtataat
    32041 tattgcttaa tgatgggccg ctttgttcca ttgttagaaa atcgggacct tgaacgattt
    32101 caccttgtac cgttttattt tccatcgttg gatattccgg accttttaca atttcacctg
    32161 taattgtgcc ctgtggaatt ttaactaatg gttgtgcaac tggttgtgtt gtttcttcag
    32221 ctttaccagc cgtagtttta acctcttgtt ggttatcaac tttaggtgct tgaggttctt
    32281 caactttctt ctcttctttt actactggcg attttgtttc agtttctccg tattttttga
    32341 cagttttctt tttccaagaa tcatctgctt ctttaactgc ttttttcgtt tcttcaacta
    32401 atttatcaaa attaggttta ttatcactat ttgttttata gttatgtgtt gtaggattat
    32461 atttcgttat agatttcggt ctattttgtt tagtttccat aaagaaatca tcaataattg
    32521 aatttaagtc atcaatcatt tcttttttaa tacgttcatt tgtaatttta tgtggattgt
    32581 ctgtatctcc aaggattaag tccagttttg ctcgtaactc tttcgcgtgc tccccataat
    32641 ccttatcacc ataatatgat acaactaatg tatcaatttc agatacgaga tcgtatactt
    32701 ccttagttgc tttatcttct tctgctgcat taaaagtttt caagtctgaa ttcttatcct
    32761 taatatcttt aacttctctg tgaaaatcat ccagtgctct ctttaatgca tcctgtagtt
    32821 cattgtattc tttcatcgaa agttcttcta aattatattt atgaaaatta gccattttta
    32881 aatctgtacg aggattttct tttttataat ttgcatacca ttgtttataa tcttcatatt
    32941 gagatttctt tctctccaaa agatattgat cttcccttaa taccttttcc aacaacctat
    33001 ctttagcttc tttataaata ttatctccat attcatattt attagttaat cctatagcat
    33061 aaattatata gtcttccaaa taatatagag ctgaatttaa atatctgcta tctattaatg
    33121 tcccattttt actcccagca ttaacttgtg atttcccact ataatccttt gttactatcg
    33181 catctgcttt gttatcccat gtaaataagc tagatgcaac tgctaatgcg cctagcgaaa
    33241 ttatttgctt tttcataatt ttttaattcc tccaaaatgt aattgcccaa tctacattaa
    33301 agaaacaaaa tattaaaaga cattaactat atattaacta gaataaacaa agcattaact
    33361 atctttttgt tatagttaat tagctttgca atatacaata gtgtataatt tttttgatta
    33421 atgtacatca aaggagtaac aaagcatgac aacacaaatg aaaatcaaaa catatttagt
    33481 tgctggtatt aaagcggcgc tccttgatac gactggtatt aaattagcaa gcaaatctga
    33541 aactacatca catacgtatc aacatcaagc gcttgtagat caattacatg aattaatagc
    33601 aaacactgac ttaaataaat tatcgtacct aaatttagat gcgtttcaaa aacgcgatat
    33661 tttagctgcg cactatattg caaaatccgc tatacgcact aaaaatttgg atcaaatgac
    33721 taaagcgaaa caaagattag aaagtattta caattcaatt tctaaccctt tgcattcaca
    33781 aaacaattaa taattcacca ataaatcatg taagtgtttg tgacgccaaa ttgccataca
    33841 aatacttgtc atatgaatat aaacgaatga aacgattgcc tatcccatag atggcaacat
    33901 taaaaagacc tctgaaggta tcatcttgat aactttagag gtctttgttt atattattca
    33961 aacaaaattc ttatagaacg atcgaagtat gtttcgtctt tcttttcttc tttaatctga
    34021 tcagctaatg ctgggtcatc tgtgataata ccatcaacat tggtttgtaa gtatttcgtt
    34081 aaatcttctt cgccgttaat agtccaagta tagacttctt tattttccaa gtgcgcttga
    34141 ttaacaagtc ttggcgaata agaaaaatct tcgatgacaa agaaatctaa tgatgtttct
    34201 ttaaaatgac caaactgcaa cggaatgata taaccacact tgagatatgg cgcttctttt
    34261 ttcaacttag tcatcacatc ataatccaaa gacatcacac gatattgatg ttcaacacca
    34321 tgctttttca aaatatcaat aacacgttgt gtataatctg ctggttcttt accatgtggc
    34381 tttaactcta ctagtagctt cacatttgat tgtttagccg tttcaataaa ttcgtctaag
    34441 gatacaaatt ttgcttcatg tccattttga cgcattttca aaccgacgat atctttgaaa
    34501 ttagattcag aaatattttt attaacacct gttaaacgtt tcaaattgtt atcatgacta
    34561 acaacaaatt gtttatcttt cgtcataatt gtatctaact caacgtattc gacattcgct
    34621 tttgcagcag ctttcaatga cggaatagaa ttttcaacgc ctttatcttc gaaaccacga
    34681 tggccaataa tggagatatt tgtattgata gtattattgt aaagtaagta catgttataa
    34741 ccgataaaac atgtcactgc aagcaccatt gaaattataa agaacctaga cttccgtttc
    34801 ggttttggat atttaaattc taagcccggt tggtctaata cattctcttg ttttaagtgc
    34861 agtactaaca cactgattaa tgataatttc gtaaataaat aatagaagaa caatgcgctt
    34921 ttcaatacaa caaataaaat tgatgagact aaaaacttat ctccttcttc atctacacaa
    34981 atagcaagat atgttgctcc tgaaataatt aatgttaaaa tcgcaccaat gatgagttct
    35041 aatataacta tttctataac aagccgaaac ttatttcgct tcgtaatttg ccaacttagt
    35101 ctcatatttt taaataacga ctggcggttt aaaatcgtta acggtagagt aaatattaat
    35161 ttaaaattta atataaatac agcaatcata aaggtaccgt aaatgattat acctttcgtc
    35221 gttttcataa gttcttccgt taaaaattta ggtatgtaaa tattttttgt taatactgaa
    35281 cttagtccta ggttggcaat gggtatcatt aacattaaat aaatgacaaa gaaaataact
    35341 ggtacaccta tgagtttacg cacatttaca aaggcatttt taaaaatgga tttaaatgta
    35401 ataatctgtc gatcaaagcc ggcataaacc atataaacta acaatgaaaa ctctacataa
    35461 atcagaaagg caacacttaa tatgaatata ataagaagta tcacactggc gggatgacta
    35521 acgatttccg tccaattgtt aatcgtaagt tggctttgcc cagctacttt taacatcata
    35581 ttaaatagta aaattaagta tgtactacta ataaaaatca tgattaactg caatagtaag
    35641 gcattaatgc taaaacgccc tttattttga tacagtaatt taaatactgc ccatatatct
    35701 ttactaattc tcttcataat cacgctccgc attgctttaa tattaagttt catcttaata
    35761 tttttcatta ctcagggtca ataaaaattt gaaaagactc atattcatat gcaagtagca
    35821 aataataacc cattcaacat cagctaaatg atgatattgg aaccccacct ttaacaagac
    35881 atcacattct ttatcagcat agctacttac aaaaacgtct tccttcaacc attgtagaag
    35941 ttgtctcata tacttactac gtcttgctaa tactcaatac tcaattgaaa aagaagcata
    36001 tgccccttca ctcttgaagt tgcatatgct ttcttttcgg tctgaattgt atttataatt
    36061 caacgggaat tttccctttg aagttaacat aacggtaggc tgctttaaca gcttcatcat
    36121 cgggcgcttc gacatcttct aattcatatg caatgcccaa tgttttccac ttatgaacac
    36181 ctaactgatg atatggcaga atttcaaact tttcgacgtt atcaagagaa ttaataaatt
    36241 cccctagttt aattaaatcg tctttatcat cagaataacc aggcacaagg acatgtcgaa
    36301 tccatacagg ttgtttcata tctgacagtt tgcgcgcgaa gttaaggatg tgtgtattag
    36361 gctttcctgt caatctaata tgtttgtcat tatcaatatg ttttatatct aataatatca
    36421 agtctgtatg tttttgtaat tcttcaaaat gcctttgaaa tgcttttgta tcattagcac
    36481 atccagccga tgtgtctaag caagtgtgca caccattttc ttttaattct gcaaataatt
    36541 tttctaagaa tggcatttgt aacaatggtt cgccaccact gactgttaca ccgccacccg
    36601 atgcatcaaa gtatggtttg tatggtaata tttcattcac catttcatca actgtgactt
    36661 ctcttgatgg ctcactaatt ttccaagtat ctggattgtg gcaatacaag catctaagta
    36721 agcatccttg tgtaaataat atatatctta atcccggtcc atcgacagta cctaaacttt
    36781 cgacagaatg taagtgtccc ttaagcatag tgctcccacc ttaaattttg ttacatactt
    36841 tcatggaatg tacgagaaat tacatctaat tgttgttcac gtgttaattt aatgaagtta
    36901 acagcgtaac cagatacacg gattgttaac tgtggatatt cttctggatg ttccattgca
    36961 tctattaatg tttcacggtt aaatacgtta atatttaagt ggtgaccaca ttgcattgcg
    37021 taaccatcta acatactagt taagttacgg ttttgatctt ctggttcttt acctaatgat
    37081 tttggtacga tactgaatgt atttgaaata ccatctttac agcaatcgta agggatctta
    37141 gctacagaac ttaatgaaga taatgcacct ttttggtcac ggccatgcat tgggtttgca
    37201 cctggagcaa atggttcgcc agctttacgt ccgtctggtg tgttaccagt tttcttaccg
    37261 tatacaacgt ttgaagtaat tgttaataca ctcattgtat gttctgaatc acgatatgtt
    37321 ttatgactac gtaatttagt catgaagcgt tctactaaat caactgcaat atcatctaca
    37381 cggtcgtcat tgttaccgta tttagggaag tcgccttcga tttcaaagtc tactacaaga
    37441 ccttcttcgt tacgaattgg tttaacttgt gcatatttaa ttgcagataa tgagtcagct
    37501 gctactgata aaccagcgat acctgttgcc attgtacgta caatttctgt atcatgtaat
    37561 gccatttcaa tacgttcata gctgtattta tcgtgcatgt agtgaataac atttaatgag
    37621 ttaatgtaaa cacctgctag ccaatccatc atttgatcaa atttcttgaa tacttcgtca
    37681 tattctaata cttcgctgtt aataccttcg aagtttggac caacttgtgc accagatttt
    37741 tcatctttac caccattgat agcgtaaagt aatgttttag ctaagttcgc acgtgcaccg
    37801 aagaattgca tttgtttacc aattgtcatc gctgatacac aacatgcgat accatagtca
    37861 tcgccatagc tttcacgcat aatgtcatca ttttcatatt ggatagaact tgttttaata
    37921 ctcatttttg cacagtatgt tttgaagttg tcaggtaaac gtactgacca taatactgtt
    37981 aagtttggtt ctggagctgg acctaagtta tctaatgagt gtaagaaacg gaatgagttt
    38041 ttcgtaacaa gtggacgtcc gtcaatacct acaccaccga tagattcagt tacccaagtt
    38101 gggtctccag agaataattc attgtaatca ggtgtacgag caaatttaac aatacgtaat
    38161 ttcatgatga agtggtcaat aatttcttga acttcgcttt cagtaataac gcctgcttta
    38221 aggtcacgtt cagcatagat atctaagaat gttgatgtac gacctaaact cattgctgca
    38281 ccgttttgtt ctttaattgc agcaaggtat gctaagtata accattgaac tgcttctttg
    38341 aagttttctg ctggacggct taaatcgaaa ccatattttt gtccaagttc ttttaattct
    38401 tttaatgcac gatattgttc tgataattct tcacgtaaac gaattacatc ttctgacatt
    38461 tctgtagaca tcgtgttgaa gtcgtgcatt ttttcttcca ttaagaaatc tacaccatat
    38521 aaagctacac gacgatagtc accgataata cgtccacgtc cgtatgcatc aggtaaacca
    38581 gtgattacac ctgctttacg gcagttcaac atttctctag aatatgcatc gaatacacct
    38641 tggttatgtg ttttacgata atctgtaaag attttttcag tttcttcgtc taattcgtaa
    38701 ccgtaagctt cacaagctgc tttcgccata cgaataccac cgaatggttg cattgaacgt
    38761 ttgaatggct tttcagtttg tacacctaca attgtttcta aatctttgtc taaataacca
    38821 gcatcatgag atgtgattgt tgaagctact ttcgtgtcca tatcccacat gccgccacgt
    38881 tcacgttctt ctttcgataa ctgcattact tgttcccaaa gtttagaagt tgcttctgtt
    38941 ggtcctgcta aaaatgaatc attaccttca taaagagtgt agtttaattg gataaactct
    39001 cttacatcta cgtgtctgtt ccatcttcca tttttaaatc cttgccaagc tgttgcatga
    39061 tttttatttg tttctaacat actaaacgcc tccatctgta taatgttgtg aatttgaaat
    39121 gctatgttaa cgttttcata gtcattgtag catgtttgtt gtgatttttt tcacataaat
    39181 tatgtacaat tttcatcaaa tgtatgtcaa aacatagaaa ctgtactaat ttgtgtaaca
    39241 gtaatatgct aattgcattc tgttacacaa cagttataat gttcactatt ttcattgctt
    39301 taaaagttaa tttaggagta tatatttgtt tataaaaaat gattgttgat aagcgttaaa
    39361 tcaacactta gaaagattta cttttatata tgaagagact ggattaaata cttttattga
    39421 cgtaaaaatt cacttttgaa ccgttcaata tcttgccgat ttttatataa cagctacaaa
    39481 taaaatataa cagtttgatt ttacagcctc ggtaaatcgt cttgacaaac aaaaattttg
    39541 tgctatcaca acatttgcaa cgtcttaaca agtcatctat aaacatttct aaatatttaa
    39601 cattacttat gcgtcattta ttgctaaaat tattgtatta aaatatacat agaattgatg
    39661 ggatatcatg aaatcaaaaa tttatatctt gctattattt ctcatttttt tatcagcatg
    39721 cgctaatacg cgtcactctg aatccgataa aaatgtatta acagtttatt ctccgtatca
    39781 atcaaacttg attcgtccaa ttttaaatga atttgaaaaa caagagcatg tcaaaattga
    39841 aattaaacac ggatctactc aagtactgct ttcaaacttg cataacgaag atttttcgga
    39901 gcgtggtgat gtctttatgg gtggtgtgtt gtcagaaaca attgatcatc cagaagattt
    39961 tgttccctat caagatacat ctgtaacaca gcaattagag gattatcgct cgaacaataa
    40021 atatgttact agttttctat taatgccaac agttatagta gtgaattcag atttacaagg
    40081 agatattaag attcgaggtt atcaagattt attacaacct atacttaaag gtaaaattgc
    40141 gtactcaaat ccaaatacaa caacgacagg ctatcaacat atgcgtgcta tttatagcat
    40201 gcatcatcga gtaagtgatg tgcatcaatt ccaaaaccat gcgatgcaac tgtcaaagac
    40261 gtctaaagtc attgaagatg ttgcaaaagg taaatattac gcaggtctaa gctacgaaca
    40321 agatgcacgc acatggaaaa acaaaggtta tcctgtatca atcgtttatc caattgaagg
    40381 aacaatgtta aatgttgatg gtattgcttt agttaaaaat gcacaccctc atcctaagcg
    40441 taaaaagtta gtgcaatatt taacaagccg ctcagtacaa caacgattag ttgcagagtt
    40501 cgatgccaag tcaattcgaa aggatgttag tgaacaaagc gatcagtcta tcgaaaatct
    40561 taagaacata cctttaatac cgaaatctaa attaccggat attccacatc ataaattttt
    40621 ggagatgatt caatgacggc atacaaacct tatagacatc aattaagacg atcgttattt
    40681 gcgtctacta tttttccagt atttttagtc attattattg gactagtaag cttctatgcc
    40741 atctatattt ggattgagca tcgtacgatt catcaacatg ttgatgaaag tcagtcatct
    40801 ttacatcata ctgaaaagca gattcagaca tttattacac agcataataa ttcgtttcaa
    40861 gagctcgatt taacaaatca tcatgatgtg accgctacaa aacgtgaatt attaaaatta
    40921 atacaccaac agcccgcaac actttattat gaattatctg gaccaaatca gtttataacc
    40981 aataattatg aacatttaaa cactaaaaat atgtatttat tttcaacgca tcagcttaaa
    41041 ttcaaaaata gtacatatat gcttaaaatt tatatggcta acaccccacg tttaagtgaa
    41101 attaagaaag ataaccgaca attcgcactt atcgttgatc aatatgacaa tattttatac
    41161 gccaacgatg accgttttac tataggtgaa aagtatagac cacaacaatt cggatttatg
    41221 aatgaatctg taaaattaaa ccatgctgac catcgtctca ttatttataa agatatacat
    41281 gaaaacattg aagatggtat cacgttgtta attgttatgg ccgtagttct tgttttacta
    41341 gtaatatttg gtttcattag cgcagataat atggctaaac gacaaacaaa agatatcgaa
    41401 acgattattc aaaaaattta ctatgccaaa aatcgtcatc taggtacata tacgccttta
    41461 aaaaacaaca gtgaactaga agaaattaat aattatattt acgatttatt tgaatcaaat
    41521 gagcaactga tccattctat cgagcataca gaacgtcgct tgcgtgatat tcagttaaag
    41581 gaaatagaac gccaattcca gcctcatttc ctattcaata cgatgcaaac aatacaatat
    41641 ttaattacgt tatctccgaa attagcacaa actgtggtcc aacaattatc gcaaatgtta
    41701 cgttactcat tacgtacaaa ctcacataca gtggaattaa acgaagagtt gaactacatt
    41761 gaacagtatg ttgcaataca aaacatccgt tttgatgaca tgattaagct ccatattgaa
    41821 agttctgaag aagcgcgtca ccagacaatt ggtaaaatga tgcttcagcc actcatagaa
    41881 aatgctatta aacatggtag agatactgag tcattggaca ttactatacg cctgacattg
    41941 gcacgtcaga atttacatgt tttagtttgc gacaatggta tcgggatgtc gtcatctcgc
    42001 ctgcaatatg tacgccaatc tcttaataat gatgtatttg atactaagca tttgggatta
    42061 aatcatttac acaataaagc aatgattcaa tatggttcac atgcacgact ccatattttt
    42121 tcaaagcgta atcaaggtac gttaatttgt tataaaatac cactttcaag ggggaatgta
    42181 gatgtttaag gtagttattt gtgatgatga acgaattatt cgagaaggat taaagcaaat
    42241 cattccttgg ggagactatc atttcaatac aatatacact gctaaagatg gtgttgaagc
    42301 attatcacta attcaacaac atcagccaga attagtaatt actgatattc gtatgccacg
    42361 taaaaatggc gtcgatttac tcaatgatat tgcgcatcta gattgtaatg tcatcatatt
    42421 atcgagttac gatgactttg aatatatgaa agccggcatt caacatcatg ttcttgatta
    42481 tttattaaaa ccagtagacc atgcacaatt agaagttatt ttaggaagac ttgtccgtac
    42541 cttattagag caacaatcac agaacggccg tagcttagca tcttgtcatg atgccttcca
    42601 accactatta aaagtagaat acgatgatta ttatgtaaac cagattgtgg atcaaattaa
    42661 gcaatcttat caaacgaaag tgactgtttc agatttaatt caacatatcg atgttagtga
    42721 atcgtatgcg atgcgaacat ttaaagatca tgttggtatt acaattgtcg attatttaaa
    42781 tcgctatcgt attttacaat ctctgcaatt gttagatcga cattacaaac actatgaaat
    42841 tgcagacaaa gtaggctttt cagagtataa aatgtttagc tatcatttta aaaaatattt
    42901 acaaatgtcg ccaagtgatt attgtaagca agcaaaatag attagcacat aactaatgat
    42961 tatggcgcct ccattttgtt ctatgaaaaa gcggactgat agagaagttt tcacttttct
    43021 ttcagtccgc tttttagaga gtttaaatat aaaacaatac tccctacaat attgacaccg
    43081 ctatatgtct ttattgtaag gagtaaattc attgagtcac cctaagtcgg gcaaataagg
    43141 atacttcata acagggaatt atgatctgcc ccgagacata gagatgatat atttgcaagg
    43201 gtagaagcag tatctttcat ctcttcacgc acatgccgtc ggggcaggat tgccaactat
    43261 attgcaatag attggcgata cttttaatcc gatttatatt aaatttttaa acttctaatt
    43321 ttcttttctt catagaaagc aacgattcct aatagaatca tgcctaggaa taatgcaaca
    43381 tagaagacga tgaaaacatc tgtccatcca cttaatgtat atccaaagat gtttaaaccg
    43441 ttacgtgttg gatcagcaat agccgccaaa ccaactttcg ccattgagtc accgaataga
    43501 tacgcgaatg aacctgtcat tccgtttgct acactgatgg catttttagg aacaaaacca
    43561 gtcaatgata caccaattaa taattgcgga ccaaagatta acgcacctaa tgcaaacaat
    43621 gaaatgttaa ccatcatgac acttgtagca tttgtgtaga ataagacaac aaatgtaatc
    43681 ataaacatac agccaatagc tacaattgca cgacgacctt ttaataagtc tgatacgtag
    43741 ccccataata aacttgcaac taatgcacca atttcaaagt agaatatcgt attaactgca
    43801 tcgcctttac taaagtgtaa atgctctgac acatataacg gtgcccagtt atcaataccg
    43861 attcgtacaa tgtatacaaa gacgtttgaa acacatagaa tccatataac aggatttccc
    43921 aggatatatt ttttaaagat ctcccatttc gtcatacctt gagaatcaat attttcttta
    43981 tcgaccggct cttcccaaat ttcttcagca cgattccatc ctaattcttc cggatcatct
    44041 tttccgataa ataatgttgc gataccaata agtaatgcaa tcaccgatgg gaaaatgaac
    44101 atccctataa catttccatg gaagaataca ttagcacccc aaagtgcaac accacctgct
    44161 atggcaccac cgatattatg tgatgtattc cagaatccta agtatcggcc acgtttcgtt
    44221 cttggcgccc atcttgaaat cgttgaataa cttgcaggtc caccaactga ttggaacacc
    44281 ccgttaagtc cccaaagtac aattaataat cccattacag aaccaaagta acttaaaaca
    44341 aatcccataa ttaaaactgt aatcgcagat aagataagta agaacgagat aatacgtttt
    44401 gtgttacgtc catcgacaaa atatccaagt aatgtttttc ctaaaccgta cgtgatacta
    44461 aatgctaatc cgatataacc aagttctaat gtagataatc caatttcctc ttttaaaaac
    44521 ggttgtgccg ccttaaagtt gtttcgaatt aaatacatag ccatataaac aaagaacact
    44581 acgaagaaag cttgcatgaa gtttctaagc cataatttac gttgtaccga taatggaatg
    44641 cctttgttcg gaatcttatg gatatcaaaa aagttcatct ctgtcacctc aatcattttc
    44701 gttaagccaa atatatcacg tcggtgtaag cgcttaatat ccttaaaatt attaaattat
    44761 ccaaaatact gaacataaac acatacttag aaacatattt atgtactaaa attaataaat
    44821 agttaatagt atttgacaag ctttatctat aaaatacttt atgttgtcaa aatcaacgct
    44881 tatacatatg acataatgac ggaggacatc atgattctat ctatattact tatctttttc
    44941 tgtatcagac ttgtcagctt aaagatatct attaatcact caaaacaatt aaaagcagac
    45001 ggtgcagttg aatatggcgt taaaaattcg aagtttttag caataacaca cgttttaatt
    45061 tatgtattgg ctggtgtaga ggcatttatt aataaagata catttagctt tgcaaatggt
    45121 attggcttag ttatattaat ctttgcttat atcatgttat ttatggttat taaaacttta
    45181 ggtggtattt ggacattaaa attattcatt ttacctaacc atcctattat taaatctgga
    45241 ttatataaaa ttacaaaaca cccaaattac ttcttaaaca tcattcctga attaatcggt
    45301 gtattattat taacacacgc aacatataca actattttat tagtaccgta tgcgtatttc
    45361 ttatatgtac gtattaaaca agaagagaaa ttaatgaata tttaaaaaga tgaaagcctg
    45421 tcatcacttt agtctacatc gacgtccatc tttaaagtgc attctgaaat attatattca
    45481 gaaaccttta aagtatgtcg tgtctatgac cattgtgaca acaggctttt ttttattatc
    45541 atatacctca ctattgaaaa ggttatatac tcttttcaat ggtgaggatt gttactgtga
    45601 atgggtatga ccatcgctat atacttatat aaagtacacc ttctttacaa acaatgactt
    45661 gatttaaggt gtgaaatttt tggcaatggc ttggcgacat atgcatttat tcaacactgc
    45721 caccagtctt acttcagaca aacttcagac taacctcgag ttcgtattat gcccaccaca
    45781 tgtctgctgg cttgtcgtaa atattaacag atttcaaagt ttgacaagct ttttggaaac
    45841 cttcttctac tgacataata ggatcttcat gttcaatact taatacataa tcatatccat
    45901 taataataag ttgacttatg atatctgccc atacatatgg actatgtcca taaccaactg
    45961 tacggaatgt ccatgctctt gtcgcaacgt taccatatgg ttgcatatca gttagaccat
    46021 acatatttac attttcttga ttaatatacg tatctttagc atggaagtga tgaattgcat
    46081 ttgcttggcc taatatgcga atcgcagcaa ttgggtcaat accttgccac catagatgac
    46141 taggatctaa gttagcaccg atatattcat ttgtagcctc acgtaacttc aacattgtat
    46201 atggtgtatg cactaaaaat cctgcatgca actctatggc aatttttaca tcttgctctt
    46261 ttgcaaactc agctaaatct tgccaatatg gtatcaactt ttcattccac tgataatcat
    46321 aaatttcaga gtaggctgtt ggccaaggtg taacaggcca attaggcttt ttagcggtat
    46381 catctgatcc tgcaatgcca gaaaatgtat taacaactgg cacgtctaat agatttgcta
    46441 aacggattgt tttacgtaac gtttcatcgg cttctttcgc ttctatcgga tctggagaaa
    46501 ttggattgtt atgacaactg aaaccactta tttgtaagcc tctgtctgtg attgacttca
    46561 taaatgcttg gcgcttgtct tcattttcta acaactcatc taacttacaa aatttatctc
    46621 ctgggttacc acctgttcca acttcaatca tatccaatcc agattctgag acataatcta
    46681 acatatcttc aaaattttta tcgtaaaata atactgaaaa tacacctatt ttcattgaaa
    46741 agcactcctt ctttttatat tgagaatttt gtatcatata tgataatcat taaagttgta
    46801 tgctcttgtt atcaagatcg ctacgataaa tcgcttctat aagggcattt acattgcgtg
    46861 cttcttccgg tttcaccaca atctcttcta tccctaaaca cgcattgaca aagttacgcg
    46921 cctgtctctc accagcaatg tcttcgttat gctcaacatt agctttgctt tcaaaaatag
    46981 ttccaaagcg gggctcatat atttcaaatg gaaataaatt gataccgcca tcttctcctg
    47041 ataaactaac gtgaacctta tcttctttga tatttgcaga ccacgaacat tcaaactgca
    47101 tgcttgctcg attggcaaat gtcatataac tagtaacatg atcatcgaca tcaaatttag
    47161 tatgatcaaa tgttccccaa tcattaattt gattcggttg tttgctcaat tgattatatg
    47221 tttttcctag cacttcatgc ggcaccatat ctttacctag tagccacaaa gataagtcta
    47281 acaagtggca accataatcg attaaactac cgccaccttg caacgcttta ttggtaaaaa
    47341 caccccagcc aggcacttta cgcctacgca tcgcttgtac acgtgctact aaaggtttac
    47401 caaccacacc tgattcaatt gcttttttag cagtaattgc cacatctgtg tgacgataat
    47461 gatatgcgac agttaataat ttgtgatttt tattagccgc ttcaatcatg cgatcacact
    47521 cttccgtcgt catcgccatt ggcttttcac acaatacatg gacaccatgg ttcaatgctt
    47581 ctatagaaag atcagcatgg aatttattag gtgtacaaat gaccaccgca tcaacaagtt
    47641 taaacagctc gctaggtgtc tcaactgcat gaggtatatt aaagcgcttc gcaacatcaa
    47701 tcatctgcac tgtattaata tcttgtactg caactaatga gactgtgtct ttgagtttca
    47761 gcaatgctgg aatatgacgg tcttgtgcaa taccaccaac acctatcaca ccaactttta
    47821 attttgtcat gatgtgcctc cttaccgtat gatgttattc aaagtaaatt gctttgcctg
    47881 attttgcaga ctgataaatc gcttcaagaa tttttgtaac taccattgct tgttccggtt
    47941 tcacaactgg ttcagtatca tttacaactg catcaatcca agcttttgct tcttcttcag
    48001 cttcatccac ttcattacct tcataaaagt cgacgccttt gttttccaat tcaacgtgtt
    48061 tggtataaag tgtacccatg tcttcaccat gaatacgtag accatctttc atatcagcac
    48121 ctgctttagt tcctgataat gaacattttg cctcatccac ttctaaagaa ttaatcgccc
    48181 aagcggattc taaaatgatc gtcgctccat tcttcatttt aataaatcca aacgcagaat
    48241 cttcaactgt aaattcatct ggattccatg aaccccaagc gtttgccgca tgatgctgtt
    48301 tatttaattt atggaatgtt gaacccatca ctgattctgg ttcataatta tccatcatcc
    48361 ataacgttaa atctaaagcg tgtgtaccga tatcgattaa tggtcctcca ccttgagctt
    48421 cttcgtctag aaagacaccc catgttggta ctgctcgacg acgaatggca tgtgcctttc
    48481 cgaagtaaat gtctcctaag tcgccacgtt gcgctgcttg atgtaaaaat tgactatctg
    48541 ctcggaaacg attttgataa cctattgtta attttttacc tgttgattta gctgtatcta
    48601 tcattttttg agcttctgct gtcgttttag ccattggttt ttcacacatc acatgtttac
    48661 cagcatgcaa ccctgctaca gtaatttcac aatgcgagtc atttggcgta caaacatgga
    48721 taacatcaat cgtgtcatct tttaacaatg ctttgtaatc atcataaacc tttgcattgt
    48781 cagttccgta tgcttctgcc gcactcgctg ctttcgaaat gtctacgtca caaaatgcga
    48841 tcatttcaac attttcaact ttttgtaaac ttggcatgtg cttgccattc gcaataccac
    48901 cacacccaat gattccaact ttaatcgtca tcataacgtc ctccttttaa tctttatcgt
    48961 cattagtggt tctttatcaa ttctgcccca ctcctaattt caaactaacc ttttgtcgca
    49021 cctgttgtta aacctgatac taaatagcgt tgcaagaaca agaatacgat tgctataggt
    49081 actgcaatca taattgcccc tgctgcaaac actgtgaaat tatttgcata cttatcatta
    49141 ataaagttga acaatccaac tgctaatgtg aatttttcag gacttcttaa tagtatttta
    49201 ggtaatataa agtccataaa tggccccata aaattgaaca aagcaacaac tgctaaaatc
    49261 ggcttagcta atggaagcat aatttgtaag aaaatacgca tatgccctgc accatcaatt
    49321 ttggcagatt catcaagttc ttttggaatc gtatcgaagt aacctttcac taaaaaggca
    49381 ttcatcggta ttgatccacc aatatatacc agtgttagtc caaataaaga atctaataat
    49441 ccaattgtat ttagcaaaat atagattgcg accattgcca ttaatacagg gaacatttgt
    49501 aaaatcaaaa atgtaatcag cccgtattta cgaccaacaa agcgatatct agaaaaagca
    49561 tatgctgtta acgtgacaaa tatcacacta aacagtgcat ttgcagatgc tacgataagc
    49621 gtatttttat accaagtcag gtattgacta ctgtcatcga atagtaagaa tgcataattt
    49681 ttaaatgttg cattgtctgg tatcattttg gcaccataca agttcgtacc tggattaagg
    49741 gaaatgccaa atgtccacag tagtggatat aaaatgatga caaacatcat cgctataaaa
    49801 ctgtaaatac cgattgcttt taatatgttt ttcttctttg tcatcttata aacctccctc
    49861 atctttaaac gtacttgtac gtctgaattg aataaatgcg acaatagcaa caataaatcc
    49921 aataattaat gacacaactg cacccatgtt gaagttgtta aactcaaatg tcagattata
    49981 cacccaagat atcaagatat ctgtactacc agcattctgc cctgacactg gtggaccgcc
    50041 tttattaaat agataaataa gattaaaatt attgaaatta cctgcatatt gcataattaa
    50101 caatggcgct gtggcgtaaa tgacatgcgg gaatgtgatg tttctaaact tttgccaact
    50161 agacgcacca tccatatctg ctgcttcgta ccaatctgat gaaatacttt gcagtactcc
    50221 agtgaacagt gcaaagacaa atgggaatcc aagccatact tgaatgccga ttaatgccac
    50281 ttttgcccaa aacggatcac ttaaccatgc tggtgctaca cctaataaag gttgcaaaat
    50341 atcattattt atcgcaccaa attcatcatt aaataacgct acaaatatta aaattgtcac
    50401 aaatgatggt acagcccaag gtaggattaa cacagtacgg ataaatttct tacctttgac
    50461 gacagggtga tttacaataa ttgccaaaaa cagccctaat gcaatttgaa gtgtcgttgc
    50521 aacaagcgtc catactaatg tccaagtaat aacactgaaa aatgttttac gccaaacgcc
    50581 aattgtgaat aacgttttaa agttatctaa accaacccat tctaatgtgt gtctcggagg
    50641 cgcgttgtat aaattgtaat ttgtaaatgc tactccaaac ataaatatta atggaaatac
    50701 aactacaaat atcaataaga atgtacctgg tgagattagt aagtatggga acgtcttgtc
    50761 ccatgttgcc accatacgcg ccttcggatc ctttatttcc tcattgcgat taaatcgttc
    50821 agcattacga tatgcatcta aaatattaat gatatatagc atgattgcga aagcaacgag
    50881 taagatagaa ataatacctt gtgcaagtaa gacacgagaa tcgtctaact taggtactgt
    50941 ccctaatgtg aacaatcccc aaaaaccaat atttaaaaag ctataaaaaa cagaaataaa
    51001 actgatgaaa aagataaaaa atatcgtccc tttaatgggt cttttattat aaaactgtcc
    51061 caaaccaggt ataacagata ataatgccgc taatttaggg ttacgtttcg tcataactaa
    51121 tctcctttct tatcattttg tgatggatga agaatcttaa tattttgcgt tatatcattc
    51181 gtcgcctcat ctaacgcttg tttaggattc ttaccatttg aaataaatat gcttgcattg
    51241 cccatcggtt cccaaacttg tcgcatttca ggaatattag gcatcggttc agcatgacgt
    51301 gcttgctttt caaacacttt taaatttgga ttagatgatt tcacgtcaac acgtccagta
    51361 atttcgctca tttcatctgt atatttttgt aatgtatctt tactagtgat atacagcatt
    51421 aaatctttag cccaatactt atgtttacta tattcagata aataccaacc acgtacacct
    51481 agaaatggtt tcataggttt gccaccatct gtaggtaatg ttgttactcc taaatcttta
    51541 ccaaacgttt cttgatattc attaatgttc cacggtccag tgacaaattg tcctactttt
    51601 ccttctttaa aaagaccaat catgacatca tgtgttgctg ccttaggaag ataccctttg
    51661 tcgtaccatt tttgtaatcg ttcagcattc ttgacgacat gttttgaatt tagtcctagc
    51721 tgatgaatat catattcact gccatttttc ttgaaaatat aatcatcatt gccgaataaa
    51781 aacggataat taaaatagaa atttttagca tcaaataaca taccgtattg tttcttttta
    51841 ctatcagtta gtttggcagc attagcttca acttcttcta acgtttgcgg tgcatttttc
    51901 actaattttt tattataaaa aagtgcggtt gtttcaacga tagctggcaa tgctagttgc
    51961 ttattgtcat aattcatcgc tttaagtgct tgcttattga aacctttcaa ctcatctttt
    52021 gataatttga tttcagcagc taagccttgt agataggcac ttccagtatt atcatgtgct
    52081 aagaaaaaga tatctggacc ttttcctgca ggagcgtcta gcgaaatatt ttctagttga
    52141 tcattttgac caatatttac aagctttact ttgatgccag tttttttagt atattgatcc
    52201 gtaatttttt tataaaacgc catttgcttg tcgccatcca cccacatcgt aagttggtta
    52261 ggcttgtctt tagaattatc tttattcaat gctttatcaa tatcttcttt cgaacgatta
    52321 gggccacatg cagttacgat taataacatt accacggcta acgtgataca ttttaaaatt
    52381 ttagacatga atactccccc ttagacgata cgatttcctg ttttttcatc aaaaaagtga
    52441 cacttattca tatcaaatgc tagtgtaatc ttgtcgttcg ccatcacttg agttcttgaa
    52501 tctaatttag aaatcaattc cattccttgg aatgtgctat gtaccataat ttctgaacct
    52561 aacagttcgg atacaactac ttcagattca aattgtgtct ctggagaagt ttgaataaat
    52621 attggttctt catgaatgtc ttcagctcga atacctaaaa taatttcctt gtctaagtag
    52681 ccagcagctt ttaacttttc aaatttttta ttatgtaatt taaaatggtg tgttcctacc
    52741 ttcaatccgt ccatttcaac tgtagcattc aacatattca ttgctggtga gccgataaat
    52801 tgcgccacaa atatgcaatt aggggcatca tatatttctc taggtgtgcc gacttgcata
    52861 atgtcgccat ctttcaaaac aacaattcga ctagccatcg tcaatgcttc agtttgatca
    52921 tgtgtaacat aaattgtcgt agtattaagt cgcttatgta atttcaatat ttctgtgcgc
    52981 atttgtactc gaagcttcgc atcaagattc gataatggtt catccattaa aaagactttc
    53041 gcatccctaa caatagctct gcccaaagca acacgttgac gctgtccgcc agataacgct
    53101 tttggtttac gaccaagata ctcagttaat cctaatattt cagctgcttc attaactttt
    53161 tgttcaatct cttttttatt tactttacgt agctttagcc caaatgccat attttcaaaa
    53221 acagtcatat gtggatataa tgcatagttt tgaaatacca tcgcaatatc tctattcttt
    53281 ggttcaacat cgttcatgcg ttccccatca atataaaaat ctccagatgt gatagactct
    53341 agtccagcaa ccattcgtaa tgttgttgat ttaccacatc ccgatggtcc aacaaataca
    53401 atgaattctt tgtcagtaat atgtagatta aaatctttca ctacagtatt gttgttatca
    53461 tacgtctttt taatatgctc taactttagt tctgccatgt tatgcctccc atactttgtt
    53521 tacagtttga ttgtgcaata cacttcgtga aaacgtttgc acaaatactt acataaatca
    53581 tatattcagt cattattaat gtcaatcata aattataaac gtttacatta ctgtcagaat
    53641 taaactttta tacagaaaat tgatatagtg cacaactatt ttctagtgtg acaataatgt
    53701 tttttatgtg cgatatttca ctgccaatgc taacaacctt gtcttacatt ttaagtaata
    53761 tctaaatcaa acaagtatga cttagcatta cgattttcat tgaaaaaagc aaccattatg
    53821 taagtatcat actcacacaa tgattgctta attttttaga ctgcgggcac ccttctataa
    53881 ctacaaatca tataggctac gttccccatc aggtaactga tcgataaacg gttttggatc
    53941 ttctgcatat gcattaccta cgccaccctt cattctttga aaatgtaaat gtgcgcctgt
    54001 cgtttgtata cctgtattgc ctgaatatgc aataatatca cctgctttga ctcgatcacc
    54061 tactttgaca ttatatttgt ctaagtgtag ataccactgg tgatattctc cattgtcttc
    54121 ggcaatctgt aataccttgc cgcccaattc attattaaag atacgtgtta ctttaccatt
    54181 cgtcggcgct ttaattggtg taccttttgg caatgcaaaa tcaatgccat aatgcttacc
    54241 atcaaatggt gaatgttgat atttaccaaa agtctcagta atccgactcc ctctaaaaaa
    54301 gtcttcccat tttgagtgtc cttgtccttt atgattttgt gtcaatttat cggtatattg
    54361 gttaaaccat ttttgaatgt catctatata aataatcgta aataatagtg aaagaaaaat
    54421 gataattgtt gccaaaatac gttttggtcg ctttgtcata gtggcatcca tccttcatat
    54481 attttgggta ttaagtatat attctaaatc aactatttca taattaaatg cactcatact
    54541 ttagtagtac tgccaaatat agatgaagaa aatgatggta atttgaatat tttgattgat
    54601 attaaatttt atatcgagta taataattat acttaagaaa ataacagaaa cagaggagaa
    54661 ttttcaaatg gcaaaagtat tatatattac agcacaccct tttaatgaac tggtatcaaa
    54721 ttctatggcg gctggtaaag catttatcga aacatatcaa caacaacatc ctgatgatga
    54781 agtgaaacat attgatttat ttgaaactta tattccagtt attgacaaag atgtattaac
    54841 tggttgggga aaaatgagta atggcgaaac gttaactgat gatgaacaaa tgaaagtttc
    54901 aagattgagt gacattttag aagaattttt aagtgcagat aaatatgtat ttgtaacgcc
    54961 tatgtggaac ctttctttcc cacctgtagt taaagcatat attgatgcta tttcaattgc
    55021 aggtaaaact tttaaatatt ctgctgaagg accacaaggt ttactaactg acaaaaaagt
    55081 attacacatt caatcacgtg gtggatatta tactgaagga cctgctgctg attttgaaat
    55141 gggtgaccgt tatttaagaa caattatgac attcttaggt gtgccatcat atgaaacaat
    55201 cattattgaa ggacacaatg ctgaacctca taagactgaa gaaattaaag caactagtat
    55261 taataatgct gaaaaattag caacaacttt ttaaattttt agttacactg tgtgacgtct
    55321 caaacgtcat gcagtttttt attgtatctc ttttccgctt tttataaata tctattattt
    55381 tcataaattt aaaatttgat attcacttta atactgatgt aaatattttt caaaatatat
    55441 tattatagtg ttaatgaagt attttgagaa acggtgggaa tgtatgaaag cacataaaat
    55501 tttctggtta aacttagcag caattattat tatttcaatt gtagtcagtg gcgatatgtt
    55561 tttggcaatg aagtgggaac aaattcattt gaaagatggt cttaaaaaag tactaagtac
    55621 gtatcctatt aaaaacttag aaacgcttta tgaaattgat ggtcacgata atccccatta
    55681 tgaaaataat gatcaagaca catggtatat agaatcctct tattcagttg ttggatcaga
    55741 tgagctttta aaagaagacc gtatgttgtt aaaggtagat aagaacacac ataaaataac
    55801 tggtgaatat gacacaacta caaacgataa aaaaaatgct actgactcga catacaaaag
    55861 ttatccagta aaagtagtta ataataaaat cgttttcaca aaagacgtaa aagatcccgc
    55921 gctgaaacaa aaaatcgaaa acaatcaatt tttgattcaa agtggtgatt taacaagcat
    55981 tttaaatagc aatgatttga aagtcacaca tgatcctacc actgattatt ataatttatc
    56041 tggtaagttg tcgaacgata atccaaacgt taaacaatta aaacgtagat ataatattcc
    56101 taaaaacgca tcaacaaagg tggaattaaa gggaatgagt gatttaaaag gcaataatca
    56161 tcaagatcag aaactttatt tttatttttc aagtcctgga aaagaccaaa tcatttataa
    56221 agaaagcctt acttataata aaataagtga acattaatac ttatgctgta attatagaaa
    56281 catccaaatc atctattaaa atcctatatt ataaaaacac ctcacataac tcgttcaact
    56341 gtaccaaacc acattacatt agattttagg ctaactattg tgatgtacat caaaaacgaa
    56401 tttgtgaggc gttgtatatt ttacaaaggt gactagcgtt tcgtatagca tttccaacat
    56461 tactacactc aagcgtcacg ctaaagttcg aaatcgaatc ctttcattca acaaaagctc
    56521 atatccacta caaacttcat atcaagcgta taaactatct tgtgatacta tctcgatcat
    56581 atctatagta tgcatttgtg ttccgtttca ctgaagtata tgtatcatca gttaagtata
    56641 aaccgtcatc cttcaatgtt acttgataag catatttccg tgctaaccag gcaatatcta
    56701 tataattttc tcctgcgttt tcataacttc ttaaatcttc aatatgtgca ctaacttcag
    56761 ggaaaatgat tctaacaaca ctttcatcaa cccaatattt gtcatgcatc catcgcactt
    56821 gatctgccaa taaaggtaac tgcacatcat tgaaatatag acgaaagccg tcactatcat
    56881 acatttgccg atatggtaat ggctgttttc taatcactaa cacctcgcca cccattacgg
    56941 tgccttctct agtatcatca cttccacccg aagcttcata cgttgttggg tcaacctgta
    57001 gtccatgtac atctccaata taagcatctg gtttatgttc cattgcatgt ccatgtgcaa
    57061 tcaatgctaa tattgtagat tgtgaaaatt gaggctccca ttcaatgcga ttaggatggc
    57121 tactataaat tctaggttca tctatagcct gctgaatatc catgccaaac actaatacat
    57181 tgattaatgt ttgcgcaaca ctagcaatga tacttatggc accaggtgca cctactgtta
    57241 atattggctt cccgtgatac atcacaatcg ttggagccat gttacttagt ggtcgtttat
    57301 atggtgcaat ttcgttaata ccaccatcta ctacatcaaa gccatccatt gtcgtattca
    57361 ataacacacc gtagcctgga atcgtgatac ctgaaccata aatcatacca attgatgtcg
    57421 taaatgaagc aatattacct tccttatcaa tcacacaaaa atgagttgtt tcggtatgat
    57481 tttcttcaac atctgtatga ctaatgcaat cagacacaac accatgctct atatcaatat
    57541 ttgctttatt gctatcaatg agcgtactgc gtgcttttaa ataatcatca tcaattaatg
    57601 actgtacagg cacctcatga aaattatcat ccgccaagta ttgcgcacga tcactatatg
    57661 ctaaatgcat cgcttgtatc aaatgatgca agtaatcaac agatcttgga cccatagatg
    57721 gtaaatcgac atgttctaat aacttcaata tttgaattac cgtgataccg ccagaactag
    57781 atggtcccat tgaataaatg tcatagtctt taaatgttgc actgattggc gctttaatct
    57841 gaatgtcata tttggctaga tcctctaaag tgattgtccc accacatgct ttgacaacat
    57901 tgactaattg tttcgcaatg tcacctttat aaaatgcatt aaacccttgt tctcttaata
    57961 tttgaaatgt cttacctaat tcgggttgta caatccaatc accttcacgc caatattgat
    58021 tttcatgcgt aaatacttgt gccgtttcat gatactttgt caatcgtgcg tgttgctggc
    58081 gcgaatattt ttcagtagcc caattggctg catgaccttc aatggctagt tcaattgcag
    58141 gattaattaa atcttccaat gacaatttag cataacgctt gtgaatataa tcaaacagct
    58201 ttggaattgc tggcacagcg acagttttac catgtgtagt catatcaaaa aatgatttat
    58261 attcgcctga atcatctaga taaaattgtt tgtctacatg ttcaggtgct gtctcacgtg
    58321 catcaaacgc agttatactg ccagtacttt gctcataata tagcaaatac ccgccaccac
    58381 caatacctga tgcaaatggt tctaacacat tcaatgccag ttgaattgca atcactgcat
    58441 ccatggcgtt gccaccttga tctaatacat ccttaccaat tttagccgca agaggatgtg
    58501 atacggaaat taacccttct ttagatgttt ttgtctgttt gtcatttaag ttaatgacca
    58561 tactatatcc tcctactttc tgttaaatat ttaaaacatt attgattaat ggctttttct
    58621 actttttcta aatcttgacg ttgctcgtta ccagtatcga caagtggtgt aatcggtgat
    58681 gcaattttaa atttatcgcc acgataaaac ttaataaatt gatcctgatc tatcgcatta
    58741 actactgctt gtctcaagtt tggatgcgtc ttaaatatac cttttttaat atttagcatt
    58801 aaaaagactg acttgcgtcc atttttgcga ataatgctta aatttttatc cgacttaatt
    58861 aaatcaaaat gtttttgatt cacatctgcc aacatatcaa ttgaatgatt tctaagttct
    58921 gacaatgcat tattcgggtc accattaaac ttcaatgtaa tatttttaat tttagctggt
    58981 ccataactac ctttttctgt ttcgttgaat cctggattac gttgaaacgt tgcttgatat
    59041 gcatttttct gtgtcataat gtatgcgcca cttgcataca gcgcattttt cccatctgaa
    59101 tttgcaggaa ttgtactgct atccccatat ccttttggat attcttgatt tacttgatta
    59161 acaaattttt tagataaaat gcctgccgaa gagtgtgtta agtaatttac ctctcgaggc
    59221 atcgattgat ctgtcgtaat tttaacaatt tgataaatac cgtctttatt atttactttt
    59281 tgaccatctg tcgttaacga tttgacgtta taagctttaa tcaacttatc atagattgat
    59341 ttatcgtcct tgtctttctc tttacgcaac tgatcgatgt cctcatcttt taatatcttg
    59401 atgtcattta tatgtttgtg catattgtaa gtattattgt taggcacaga ctttttatca
    59461 cgtgctctat ctaaagaaaa cttaacatct tcagccgata cacgctctcc agtattacgt
    59521 gcttgtccat tgaccacttt cgcaaaataa tcatcatctc ttaacaagaa ataaaatgct
    59581 ttattgtcct tattcacagc ataatcatga cttaacgaac ctttcgttgt taaatgatca
    59641 ttttcatcta ataataataa ccttgtgtac atattcatat taattgaata tactgacggc
    59701 gcaattgaac gtattggatc caatgtagga atttcaccat cttgttgtgt catcacaagt
    59761 ggccgcgtat ctcgttctct actattgttg taatcaaatt gttgccatat taatgcacgt
    59821 gaatttggca atccaacact atttttatct aacactttat tgtcatatac taaattcttt
    59881 tttgatccat ataaaggcgc catataccct ttatcaaata caacttcatc ttcaatttgc
    59941 ttatatgttt gtttaacatc tgcttcattt tgagtagaag ctttatttaa caactggtct
    60001 acatgtttat ctttcaataa actatttgat cctgtagaac taaataatgc cgtcatagca
    60061 tagttcgggt caccaaacac tgtcatccag tcatcaattt ggatatcata attgccggct
    60121 tgacgttgtg tacgatagct accataatct ggttggatat tcatcttcac gttaaatcct
    60181 gcattttcca attgatcttt aacgatattc atatcatttt cataacttgc ttgtcctagg
    60241 aaatgtattg ttggtcgctc gcctttcact tcaactttcg atgacttttg agccacttct
    60301 gatttcgtag ggacaccaca accacttaat accaacgcta aaactataat tgcgatacta
    60361 atgattttct tcacatctat ccctaccttt ttaatgaatt cttggatcta gtgcatcacg
    60421 cactgcatca cctataaaat taaatgctaa aacgacgaac ataatacaaa caccaggtac
    60481 aatagctaaa ttactgtgcg tttccaagta gttactaccg gtacgtaaaa tgttgcccca
    60541 ttcagctaca tcaggtgcaa caccaagtcc taggaaactt aaactacttg ttgttaatac
    60601 aaccacacct atatttaatg aaaaacgtac aatcataggc gcaatcgcat tcggtaaaat
    60661 ataacgccat atgatattcc aagtgttttc accagtgata cgtgctgcat ctacatattc
    60721 catgcgttta atttctaaaa cactggcacg cattgtccgt gcaaatgatg gtatattacc
    60781 gatacttaaa gcaataatta aatttggaat acttgctcca aatgatgcaa taattgccac
    60841 cgctaacaat aatgatggaa ttgcaaacac tacatctaaa attcgcatta ttaaattatc
    60901 aatatgatta aaataacctg cgatagtgcc tagtaacaca ccaaaaataa ctgcaataac
    60961 tactgaaata attgaaattg aaaatgtcag cttcgttcct acaactacgc gtgtaaataa
    61021 gtctctaccg aaatcatcag taccaaacgg ataggctaga ctcggtccat gtaacagtgc
    61081 attgaactga tttttagtag ccaatgtcgt atcaaatgta aattgtgaca caattgataa
    61141 tgtcagcatg tagactaaaa taagtaaccc gataatcgca atacgatgtc tagtagtttt
    61201 tcgtataaac gattcccacc cgttataact atgtatttgc gatgtacgtt ggtaacgtct
    61261 aatacttaca aacattaata atgtaaatac gttgcctgtt aatgtcatca acaataacaa
    61321 cacttcgacg atacgtcgcc ataggtcatg atgcttccat gtttgttccg ttgttaaaat
    61381 aataattaaa atgatggtta aaacgattag caatgtttca gcaatataga acgtatcggc
    61441 cacataacct ttaaaaagat ttaatgcact cgttaatata actaaaatat aagttgctat
    61501 ggcgtaactt gcgaataatt ttaaggaagc tatctttgaa ttaagttgtg ccatatgcct
    61561 cacttccttt cgttgatttc actacgtaat tttggatcga ttaaagcata aaatatatca
    61621 ataattaagt ttgctaaaga tattacaatt gatatatata cgaccccacc catgactgct
    61681 ggaatatcag gtattagttg tttttggacg atataacgcc cgataccatt aatgttaaat
    61741 acttgttccg tcactgctga accgcctagt aactctgcca ctagaagacc aactaacgtt
    61801 acaattggaa taatggcatt tttcaaaata tgtttaataa caacttgtgt cgtcgataat
    61861 ccttttgcat aagcagttaa aacataatcg ctgcgcatta cttcaagtac agaagacctt
    61921 gtcatacgcg tgatagaagc agcaatactt gttccaatga caagtacagg taaaatcaac
    61981 gatattggat gttctggcat ataagatggt ggcaaaatat ccaatttcaa tgagaacgct
    62041 aaaatgaata atagcccttg ccagaaactt ggaatagata aaccaattaa tgcaattatc
    62101 attaacgtga tatcaagcca actatttcgc ttcatcgcac tgataatacc aattggtatt
    62161 gcaataatta atgccaccat tagcgctaat actgcgacaa ttattgtaat tggaattctt
    62221 tcgccaactg ctttagtcac aacctcattc cctttgtaag tcgtacctaa gtcaaaggta
    62281 aaaacaccct tgatggtatc ccacaattga ataaaataag gttcgttaag atgatgtaat
    62341 acattgaatt gatgtatctg tgcctttgtt gcattttgtc ccagtatgct ataagccgca
    62401 tcaagcggtg aaaaatacag aatggtaaac acactgacaa taacaccaat gatgacaatc
    62461 acagccatga caattcgttc aaaaatatat ctaactaatg gctgtaaata aaaagtcaat
    62521 aagatgaaca tcggcaaggc caatatcact ttgatcatga tgaacttatg aaataataca
    62581 ttttcaaagt atgttgaaaa atgtgcttgt tcaatattct ttgaactcgt attagaactt
    62641 tgtgccttga atatttttaa tgcttcttta tgtatttgtg tggatgactt ttgctgcgat
    62701 aaatatttat atttttgatg taacgcctgt tcaatttctg aaatttcaga attattagcg
    62761 taaaaatttt tcctcttagc agaaaagaaa aactttatca ctgcatataa aaatattggc
    62821 aagcttaata ccgataatac aaacttgtaa aatgccttgt gatacaacga cttaaaagat
    62881 tctaaataag taattatgat aatcactcct tgtaaaataa tcttgagtag attactatga
    62941 tatacaaaag tatagaataa atttacacat ttgtgaatag ggaggcacaa catcatgtca
    63001 aatttattag aagtcaacag tctgaatgta caattcaatt atgatgaaac tacagttcaa
    63061 gcggtaaaaa acgtctcttt cgaattacga aaaaaacata tcctaggtat tgttggtgaa
    63121 tcaggatcag gaaaaagtat taccgctaaa tctattttag ggctactacc agattatcca
    63181 gatcacacat taacaggaga aattattttt aatgggcaat cgttaaataa tttatcaact
    63241 tcagcgttac aacaaattcg aggtaaggat atttcaatga tttttcaaga tccactctct
    63301 tcgttgaatc caagattaac gattggcaaa caaattacag aagtaatatt tcaacataaa
    63361 cgtgtatcta aatctgaagc aaagtcgatg acaatagaca ttttagaaaa agtaggtata
    63421 aaacatgcaa ctcgacaatt tgatgcttat ccacatgaac tttctggtgg tatgcgtcaa
    63481 cgtgtcatga tagcaatggc attgatttta aagccacaaa ttttaatcgc agatgaacca
    63541 acaacggcat tagatgccag tacacaaaat caattactgc agttaatgaa gtccctttat
    63601 gagtacacag aaacatctat tatttttatc actcacgatt taggcgctgt gtatcaattt
    63661 tgcgacgatg tgattgtaat gaaagatgga agtgtcgttg aaagtggcac ggttgaaagt
    63721 atttttaaat cgccacaaca tacctataca aaacgcttaa tagatgcgat tcctgatatt
    63781 catcaaacgc gtccgccaag accgttaaac aatgatattt tattaaaatt cgatcgcgtg
    63841 agcgtggatt acacatcacc gagtggcagc ctataccgag cagttaatga tattaacttg
    63901 gctattagaa aaggcgaaac attaggcatt gtcggtgaat cagggtcagg gaaatcgaca
    63961 ttagctaaga cggtcgtcgg tctaaaggaa gtgtcagaag gctttatttg gtataacgaa
    64021 ttaccattaa gtttatttaa agatgatgaa ttgaaatctt tacgacaaga gatacaaatg
    64081 atttttcaag atccattcgc atctattaat ccaagattta aagtcattga tgtgattaaa
    64141 cgaccactaa tcattcatgg gaaagtcaaa gataatgatg acattattaa aactgtcgta
    64201 tcgttgttag aaaaggttgg cctagatcaa actttcttat atcgctatcc acacgaatta
    64261 tctggtgggc aacgtcagcg tgtaagtatc gcgagagcac ttgctgttga acctaaagtg
    64321 attgtttgcg acgaggcagt gtccgcttta gacgtttcaa ttcaaaaaga tatcatcgag
    64381 ttattaaaac aattacagtt agacttcggc atcacttatt tattcatcac acatgacatg
    64441 ggtgttatca atgaaatatg tgatcgcgtt gcagttatga aaaatggcga aatcgttgaa
    64501 ctgaataaca cagaagatat tatcaaacat ccgcagtcag actatgcaaa gcaacttatt
    64561 tcagaagtag cagttattgc taaataaaag tcatgcgttg tgcaacttta tcactgtatg
    64621 gtctgaaata aattgcgcga cttctgatgt gttatgttag atgactttgt catactaact
    64681 atattgtgct aagttagggc tcggcaccat atcaagtttt aggtgctttg ccatgattta
    64741 agagtcaccc ccatactttg ggcattttaa cgccagaata aatcccccgc cactatgtga
    64801 agtgcggggg attatttata ttttattaga atattcagat ttttgagtgt gtcaacttag
    64861 cttagtcaat gtatatttaa cgtcacttac tctttttctt tcataattaa cacattcaaa
    64921 taaactttga tcaaaaaaca caaagttaaa agtaccatct tgtaatatgc tctcatacat
    64981 tatcccgtca tatttaaggc ttcgaatata atcagctaaa tattgaaatg gcaaataatc
    65041 tattccttgt tcatcgcttg gatttgttat tcctttatga atctttttta atgtttggta
    65101 atttacaaaa tactttctaa atccatcatc gccagctttg attgcattac tagttaaatt
    65161 agttaaattc gcaattttca atttctcttt tgtcacgttt ttttgtaact taaccttacc
    65221 tatataaata atgtcattat gcttaggttt aacttcttct atactgacct gttcttttgt
    65281 actaaggtat aatacgctta tccatttaga attcaatctt cctgccgttg caaatccctt
    65341 tggtggtgac attagttcac ttttctctgt aatgaactta actattctag atctatataa
    65401 tggttcaaat ctttctctaa attcctcaat actatagtaa ttagtagtga tatcgagaaa
    65461 gaacgctaaa ttctctaaat tgatcatatt attatgaaat ctatttttat acttcaagct
    65521 ctcacaaaat ccatcccagt cattatttgc tacaattaga tttttatttg tatatttttt
    65581 atcgtttatg attttagcgc ctactaaatc ttccaacact cgtctatcta aattttcatc
    65641 atctttaaaa agttcattta aaatacaact tatttgagct tcctcaacat taaatatact
    65701 ccagtcgtct tttaatgcta tttcaatctt tttaccttct tttgggctaa aagtatctgg
    65761 taaa
//
LOCUS       DAKTGS010000004        51537 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.004, whole genome shotgun sequence.
ACCESSION   DAKTGS010000004 DAKTGS010000000
VERSION     DAKTGS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 51537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.004"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..303
                     /gene="deoC"
                     /locus_tag="O0286_000183"
     CDS             <1..303
                     /gene="deoC"
                     /locus_tag="O0286_000183"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0168587.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
     gene            582..1883
                     /locus_tag="O0286_000184"
     CDS             582..1883
                     /locus_tag="O0286_000184"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCY0168588.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1897..2247
                     /locus_tag="O0286_000185"
     CDS             1897..2247
                     /locus_tag="O0286_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168589.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2399..2869)
                     /locus_tag="O0286_000186"
     CDS             complement(2399..2869)
                     /locus_tag="O0286_000186"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCY0168590.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            3180..4364
                     /locus_tag="O0286_000187"
     CDS             3180..4364
                     /locus_tag="O0286_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCY0168591.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4364..5557
                     /locus_tag="O0286_000188"
     CDS             4364..5557
                     /locus_tag="O0286_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCY0168592.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            5956..6627
                     /locus_tag="O0286_000189"
     CDS             5956..6627
                     /locus_tag="O0286_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCY0168593.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(6757..7560)
                     /gene="coaW"
                     /locus_tag="O0286_000190"
     CDS             complement(6757..7560)
                     /gene="coaW"
                     /locus_tag="O0286_000190"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCY0168594.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            7984..8844
                     /locus_tag="O0286_000191"
     CDS             7984..8844
                     /locus_tag="O0286_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0168595.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            8956..9486
                     /locus_tag="O0286_000192"
     CDS             8956..9486
                     /locus_tag="O0286_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCY0168596.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            9822..11432
                     /locus_tag="O0286_000193"
     CDS             9822..11432
                     /locus_tag="O0286_000193"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCY0168597.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(11541..12062)
                     /locus_tag="O0286_000194"
     CDS             complement(11541..12062)
                     /locus_tag="O0286_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCY0168598.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            12280..13140
                     /locus_tag="O0286_000195"
     CDS             12280..13140
                     /locus_tag="O0286_000195"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCY0168599.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            13606..14865
                     /locus_tag="O0286_000196"
     CDS             13606..14865
                     /locus_tag="O0286_000196"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0168600.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            14954..15289
                     /locus_tag="O0286_000197"
     CDS             14954..15289
                     /locus_tag="O0286_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0168601.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            15527..16954
                     /locus_tag="O0286_000198"
     CDS             15527..16954
                     /locus_tag="O0286_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0168602.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            17201..18517
                     /gene="rho"
                     /locus_tag="O0286_000199"
     CDS             17201..18517
                     /gene="rho"
                     /locus_tag="O0286_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCY0168603.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            18635..18889
                     /locus_tag="O0286_000200"
     CDS             18635..18889
                     /locus_tag="O0286_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCY0168604.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            19236..19835
                     /locus_tag="O0286_000201"
     CDS             19236..19835
                     /locus_tag="O0286_000201"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCY0168605.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            19836..20912
                     /gene="prfA"
                     /locus_tag="O0286_000202"
     CDS             19836..20912
                     /gene="prfA"
                     /locus_tag="O0286_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCY0168606.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            20899..21735
                     /gene="prmC"
                     /locus_tag="O0286_000203"
     CDS             20899..21735
                     /gene="prmC"
                     /locus_tag="O0286_000203"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCY0168607.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            21819..22865
                     /locus_tag="O0286_000204"
     CDS             21819..22865
                     /locus_tag="O0286_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCY0168608.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            22862..23281
                     /locus_tag="O0286_000205"
     CDS             22862..23281
                     /locus_tag="O0286_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCY0168609.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            23388..23912
                     /locus_tag="O0286_000206"
     CDS             23388..23912
                     /locus_tag="O0286_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCY0168610.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            23939..25177
                     /locus_tag="O0286_000207"
     CDS             23939..25177
                     /locus_tag="O0286_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCY0168611.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            25205..25834
                     /gene="upp"
                     /locus_tag="O0286_000208"
     CDS             25205..25834
                     /gene="upp"
                     /locus_tag="O0286_000208"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCY0168612.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            25858..26985
                     /gene="wecB"
                     /locus_tag="O0286_000209"
     CDS             25858..26985
                     /gene="wecB"
                     /locus_tag="O0286_000209"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCY0168613.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            27053..27505
                     /locus_tag="O0286_000210"
     CDS             27053..27505
                     /locus_tag="O0286_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCY0168614.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            27526..28254
                     /gene="atpB"
                     /locus_tag="O0286_000211"
     CDS             27526..28254
                     /gene="atpB"
                     /locus_tag="O0286_000211"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCY0168615.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            28297..28509
                     /gene="atpE"
                     /locus_tag="O0286_000212"
     CDS             28297..28509
                     /gene="atpE"
                     /locus_tag="O0286_000212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCY0168616.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            28707..29228
                     /locus_tag="O0286_000213"
     CDS             28707..29228
                     /locus_tag="O0286_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCY0168617.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            29228..29767
                     /locus_tag="O0286_000214"
     CDS             29228..29767
                     /locus_tag="O0286_000214"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCY0168618.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            29789..31297
                     /locus_tag="O0286_000215"
     CDS             29789..31297
                     /locus_tag="O0286_000215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCY0168619.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            31328..32194
                     /locus_tag="O0286_000216"
     CDS             31328..32194
                     /locus_tag="O0286_000216"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCY0168620.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            32216..33628
                     /gene="atpD"
                     /locus_tag="O0286_000217"
     CDS             32216..33628
                     /gene="atpD"
                     /locus_tag="O0286_000217"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCY0168621.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            33648..34052
                     /locus_tag="O0286_000218"
     CDS             33648..34052
                     /locus_tag="O0286_000218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCY0168622.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            34390..34482
                     /locus_tag="O0286_000219"
     CDS             34390..34482
                     /locus_tag="O0286_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168623.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            34614..34847
                     /locus_tag="O0286_000220"
     CDS             34614..34847
                     /locus_tag="O0286_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCY0168624.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            34958..36223
                     /gene="murA"
                     /locus_tag="O0286_000221"
     CDS             34958..36223
                     /gene="murA"
                     /locus_tag="O0286_000221"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0168625.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            36257..36697
                     /gene="fabZ"
                     /locus_tag="O0286_000222"
     CDS             36257..36697
                     /gene="fabZ"
                     /locus_tag="O0286_000222"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCY0168626.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(36756..37196)
                     /locus_tag="O0286_000223"
     CDS             complement(36756..37196)
                     /locus_tag="O0286_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCY0168627.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            37390..37737
                     /locus_tag="O0286_000224"
     CDS             37390..37737
                     /locus_tag="O0286_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0168628.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            38174..38869
                     /gene="sceD"
                     /locus_tag="O0286_000225"
     CDS             38174..38869
                     /gene="sceD"
                     /locus_tag="O0286_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCY0168629.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      39073..39175
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            39255..39944
                     /gene="tenA"
                     /locus_tag="O0286_000226"
     CDS             39255..39944
                     /gene="tenA"
                     /locus_tag="O0286_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCY0168630.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            39937..40767
                     /gene="thiD"
                     /locus_tag="O0286_000227"
     CDS             39937..40767
                     /gene="thiD"
                     /locus_tag="O0286_000227"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0168631.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            40751..41542
                     /gene="thiM"
                     /locus_tag="O0286_000228"
     CDS             40751..41542
                     /gene="thiM"
                     /locus_tag="O0286_000228"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCY0168632.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            41544..42185
                     /locus_tag="O0286_000229"
     CDS             41544..42185
                     /locus_tag="O0286_000229"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCY0168633.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            42271..43143
                     /gene="yidC"
                     /locus_tag="O0286_000230"
     CDS             42271..43143
                     /gene="yidC"
                     /locus_tag="O0286_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCY0168634.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(43956..44603)
                     /locus_tag="O0286_000231"
     CDS             complement(43956..44603)
                     /locus_tag="O0286_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0168635.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(44631..46115)
                     /gene="cls"
                     /locus_tag="O0286_000232"
     CDS             complement(44631..46115)
                     /gene="cls"
                     /locus_tag="O0286_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0168636.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            46281..46574
                     /gene="csoR"
                     /locus_tag="O0286_000233"
     CDS             46281..46574
                     /gene="csoR"
                     /locus_tag="O0286_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCY0168637.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            46586..46795
                     /locus_tag="O0286_000234"
     CDS             46586..46795
                     /locus_tag="O0286_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCY0168638.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            46923..47060
                     /locus_tag="O0286_000235"
     CDS             46923..47060
                     /locus_tag="O0286_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168639.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(47324..48526)
                     /locus_tag="O0286_000236"
     CDS             complement(47324..48526)
                     /locus_tag="O0286_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCY0168640.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            48844..49914
                     /locus_tag="O0286_000237"
     CDS             48844..49914
                     /locus_tag="O0286_000237"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCY0168641.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            49929..51287
                     /locus_tag="O0286_000238"
     CDS             49929..51287
                     /locus_tag="O0286_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCY0168642.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
BASE COUNT        18345 a         7553 c         9893 g        15746 t
ORIGIN      
        1 gtaaaagtga ttattgagac ggtattgttg gaccatgacg aaattgtaaa agcgagtgaa
       61 ttaacaaaaa cggctggtgc ggacttcgtt aaaacttcaa caggttttgc aggtggcggt
      121 gcgactgcag aagacgttaa attaatgaaa gatacagtag gtgctgatgt agaagtaaaa
      181 gcatcaggtg gcgtacgtaa tttagaagat ttcaataaaa tggttgaagc aggtgcgaca
      241 cgtattggtg cgagcgcagg cgttcaaatt atgcaaggtt tagaagcaga ttcagattac
      301 taattttata aaaatatgta agcatgggac atcgtaacca tatataagta tgtcgatatc
      361 agctttaaga catcataaat gccatgatga ttctaagata atttaatagg gactttaacg
      421 ttataaccaa tctatttaag aataataaca tcgcattgaa attgtgatat taaaaatgaa
      481 atcgatagct tgctataata attttcgctt gtgcttttaa aaatggacta gagaaagcgc
      541 agatgctttg tcctaagctt tttaagaaaa ggatgataaa aatgagaatg atagacatta
      601 ttgagaaaaa gcgtgacggt catacactaa caacggaaga aattaatttc tttattggcg
      661 gctatgttaa aggggatatt cctgattacc aagcatcaag tttagcaatg gcgatttatt
      721 tccaagatat gaatgatgat gagcgtgcag cattaacgat ggctatggtt aattctggtg
      781 atatgataga tttgagtgat attaaaggtg tcaaagtaga taagcactca acaggtggtg
      841 taggagatac aactacattg gttttagcac cattagtagc agctgtagat gttcctgttg
      901 caaaaatgag tgggcgtgga ttaggtcata caggtggtac gattgataaa ttagaagcaa
      961 ttgatggttt tcatgttgaa atagatgaag caacatttgt gaaattggtt aatgaaaata
     1021 aagtggcagt tgtaggacaa tcaggaaatt taactcctgc agacaaaaaa ttatatgcct
     1081 taagggatgt tactggtact gtcaattcaa taccattaat tgcctcttca attatgagta
     1141 aaaagattgc tgctggtgca gatgcaattg tattagatgt aaaaactggt agcggtgcat
     1201 ttatgaaaac attagaagat gctgaagcat tagcgcacgc aatggtacgt attggtaata
     1261 atgtgggacg taatacgatg gcgattattt ctgatatgaa tcagccactt ggacgtgcga
     1321 ttggcaatgc gcttgagtta caagaagcaa ttgatacgtt gaaaggacaa ggtccgaaag
     1381 atttaacaga acttgtatta acattaggtt ctcaaatggt tgtacttgca aacaaagctg
     1441 aaacgcttga agaagcgaga gcgttgctaa ttgaggcaat taattcaggt gcagcattag
     1501 aaaaattcaa aacatttatt aaaaaccaag gtggcgacga aactgttatt gaccatccag
     1561 agcgtttgcc acaagctcaa tatcaaattg aatataaagc taaaaaatca ggttatgtga
     1621 ctgaattagt ctctaacgat ataggtgtcg cttcgatgat gttaggagcg ggacgtttaa
     1681 caaaagagga tgatattgat ttagcggttg gtattgtttt aaataaaaaa attggtgata
     1741 aagtagaaga aggagaatca ttattaacga ttcacagtaa tcgccaagat gtagacgatg
     1801 ttgttaaaaa attagattca agcataacaa ttgctgatca tgttgtatcg cctacattaa
     1861 ttcataaaat cattacagaa taggagttga agctgattga acatactaag atatttatta
     1921 ggaataggtt ttagtgtaat aggtgtattg cattttacac gagaacgaca atttagaaat
     1981 atcataccga aatgtttgcc acttcgaaaa acagcggttc ttgttacagg aatctttgag
     2041 atattatttg gacttgcact tttaattaaa agaccatctc aatgcttgaa aaatacaatc
     2101 aatctattct tgctagctgt attcccagct aatatctata tggcagttaa aaagttacct
     2161 ttaggagata aacaattgcc aacatggcaa ctatatttaa ggttacctat gcagttcgtg
     2221 ttaatgggac taattaagaa attatagtgg ttctcaaata aatcggacaa atgttaaaga
     2281 ttttactttt tcatttgttc gattttttga ctttgaagca taataaaaag cagttggaca
     2341 atggcgattg taaaatcatt gttcgactgc ttttctgatt aacttgattg actaagattt
     2401 attttcctgt accgaaaaca tcatgccatt cgtttcgttt gtctagaaat gcttgagcga
     2461 tagttttagc accttctaat gaatgacttg ctgcccagcc acattgtact tcattacaag
     2521 caggcacttc agtagcattt agcacatcat ttaaagttgc ttcaacaata tttaatacat
     2581 catcataatt atcatgatta ataaatgata catagaaacc agtttggcaa cccataggac
     2641 ttaaatcaac aactttgtca ctatgatttc taatattttc agccattaaa tgttctaatg
     2701 aatgtagtcc gggcatatcc atatgttctt tgtttggttg tttgaaacga atgtcgtatt
     2761 tgtgaatgac atctccgttt aatccttcca tcgttcccgc taatctaata aatggggcaa
     2821 ccaccttagt atgatctaaa ttgaaacttt caacattcat ttttgtcatt ttgaatttcc
     2881 tcctattaac tactcaaaac tataaaacgc ttatgatttg ttgcattata aaacttaagt
     2941 ttgttgtaag ttgtgtttct aacaatactt aaattgtaac aatagggctt atgttttaca
     3001 atttgaaact gttttataaa tttatgaaag gcttttagtt attagaaaat tttgtaaata
     3061 aagaaaaact agatgacatc atgagataaa accgatagaa taagtaataa ttcattttga
     3121 aagaacaaaa tattgtggat gattaaaatg agtgtagggt aaaagggaga tagtaaaaaa
     3181 tgagtgaaaa acaacaaatt ctcgattata tagaaacaaa taaatatagt tatatcgaaa
     3241 tcagtcatag aattcatgaa cgtcctgaac ttggtaatga agaaatattt gcgtctcgaa
     3301 ctttaataga tcgtttgaaa gagcatgatt ttgaaattga aaccgagatt gctgggcatg
     3361 caactgggtt tatagcgaca tatgattcag gacttgacgg gccagctata ggttttttag
     3421 cagaatacga tgctttgcca ggattgggtc atgcttgtgg tcataatatc attggaactg
     3481 caagtgttct tggtgcaatt ggtttgaagc aagtgattga ccaaattggt ggtaaagtag
     3541 tcgttcttgg atgtccagct gaagaaggtg gggaaaatgg tagcgctaaa gcttcttatg
     3601 tcaaggctgg tgtgattgat caaatagaca ttgccttaat gattcatccg ggaaatgaaa
     3661 cttataaaac gattgatact ttggcagtgg atgttttaga tgttaaattt tacggaaaaa
     3721 gtgctcatgc ctctgaaaat gcagatgaag cgttaaatgc attagacgct atgattagtt
     3781 attttaatgg tgtagcacaa ctacgacaac atattaaaaa agatcaacgt gtgcatggtg
     3841 tgattttaga tggcgggaaa gcagctaata ttattccaga ctatacacat gctcgttttt
     3901 atactagagc aatgacgcgt aaagaattgg atatattaac agaaaaagta aatcaaatcg
     3961 cacgtggagc tgcgatacag actggttgtg attatgaatt tggtcgaatt caaaacggtg
     4021 tgaatgaatt cattaaaacg ccgaaattag atgatttatt tgctaaatat gctgaagaag
     4081 ttggtgaagc agttattgat gatgattttg gttatggctc tacggataca gggaacgtaa
     4141 gtcatgttgt gccaacaata catcctcata ttaaaatagg atcacgtaat ttagtaggac
     4201 atacgcatag atttagagaa gcggctgcga gtgtacatgg tgatgaagca ttaattaaag
     4261 gcgctaaaat aatggcgttg atggggttag agttaattac aaatcaagac gtttatcaag
     4321 acattattga agagcatgcg catttgaaag ggaatgggaa gtaatgacaa ataatggtaa
     4381 tgagccgaac ttaacgttaa gtgatttata tgacaaagat gttgtctata cgtcaagacc
     4441 ttcgtatata tcgaatccat ggttgaaacc ggacgaacat caatctaatt ttttaactgg
     4501 tagggagtta ttaatagcaa atcaattacc tgttattgtt cacgaagcta gtgcgacaga
     4561 caaattgcat cagctatttc aagttattgg taaggaagta ccgaactcaa tttatacatt
     4621 taacaatcag caatcttacg aaaacttgat caaacaattg gcacataagg aaaataaaaa
     4681 gatttatttt caatatattc atgatgaaac aatcttgaat cagcaatatt atgcattaga
     4741 taaaacgtta tttgttgctt taaataataa agcaagaata cctgaatgga caaatgggaa
     4801 gtttttacca aaaagaaaag ttgtaaaaat tgagcaattc gaaaacgaga ttaagaattg
     4861 ggaattcccg ttagtaatta agccgggaga tgatcttccg acagcaggtg gctatggcgt
     4921 tatgatttgt tatcatgatg cagatttaca aaaagcgata acgcgtatta aagaggcaac
     4981 tgctgaaacg aattcgctta ttatcgaaca gaagattgaa gaaaaagcga attattgcgt
     5041 tcagtttgca tactcggaat cgctaggcat tcaatatctt ggtgcggcaa cgcaattaac
     5101 ggataagtac ggattttata acggaaatga aaatactact aatgtaccag agcacgttat
     5161 agaagcaggt agacaaatta tggaaaacgg tgtgaatcaa ggattctttg gtgtagctgg
     5221 ttttgaccta ctcgtcgatg aggatgataa cgtttatgcg attgatttaa actttagaca
     5281 aaatggttca acgagcatgt tattacttgc taacgagttg aattcaggat atcaaaagtt
     5341 ttatagttat cattcaaaag gtgataacac acatttcttc aatacgattt tgaaatatgt
     5401 caaagaaggt agtttatacc cgttatctta ttatgatggt gattggtacg gtgaagataa
     5461 agttaaatca aggtttggct gtatttggca tggtgattca aaagaaacag tactggagaa
     5521 tgaacgcgca tttttagctg aacttgaaca ctattagagt tcggaacata aggcgctaca
     5581 atgttgtgtt gccagtagtt gactgaatat gcgtttgtaa caagcttttt tcgattctag
     5641 tcaacagtaa ttaaatttat gatatggcaa tactttgtaa tactaatatt aaatggcgac
     5701 ttttatttca ctatgttata agagttgcca ttttgttgat aaaggtatac taaaggttat
     5761 cgttttgaaa tttttagtaa ctagatatgt ttcgtgttat agaccgaatt tgtgtatacg
     5821 taaaatttaa tgctattgaa tttttaaaat gaaaaacatg acattaaatt gaattcataa
     5881 tatgtctaat tgactaactt gttggagtca tttactattt tatgtatgac atattttaaa
     5941 aagtgagggt caagcatgtc ttataaagca tatccattct ttagagatat attaataaat
     6001 gaatgtattt atttcgcctc taaaaataaa aaactagtac gcctaaatta taaaagtgaa
     6061 gcgtatgtag gcgtttggac agaagaaagt gtggccgtat catttttaac aagtcgtgat
     6121 attccatttg ataaagttgt aaaaatggac gttgatcgtt ttgctactta tgaattagat
     6181 gaattgtttg atgaacaaga ccatattatt atgaatcaaa caatggaaga agaagggcat
     6241 ctactaaacg ttgtagctgt tacacaagaa gtgatgacgg aattagataa aattagaatc
     6301 aaagaatttg tccaagatgt agcgaaatat gatgaagtat acggcttaac taaaaaaggt
     6361 agtaagcagt ttattctcat tagtgaaaat gatagcgacg aaaaaaagcc gcatattatg
     6421 cctgtatgga gtattaaaaa cagagcgtta aaagttcgag atgaagattt tgaagagtgt
     6481 gatttaatta cgattgaagg ttctgttttc ggagaatggc tagatgaact tagagatgat
     6541 cataaagccg ttgcgataga tttaaaaact ggcgtggttg gtacaattgt ttcagcgcaa
     6601 aaattgtcta atgaacttac attttaattg aattgagttg taagtaatta agtgataata
     6661 aatggaacaa tacgtattca aaacacttag accataaaat aaaaggccat ttatatagcg
     6721 tttatttaaa acaacgcgca tataaatggt ctttttctat ttttctaaat ataatgcacc
     6781 aatagcacct gaaaatgcgc cgttttcaac atagtacggt ttgcaaccgc gtaacacagt
     6841 ataatcttcc acaactttgc gtaataaagc gttattatga aatgaagaac cgatataaac
     6901 gatattttca gttttaaatt cacgtgcaac agtaatggcc attgtcgtaa caacttcgcc
     6961 aacgacacca ataacggctg ctaatttatt gctaggtgta aaatcagcat ctaaatgatg
     7021 tagtacatga ccaaaattag ctgctgttaa atcaccggga atgggtggtt cggtatcttt
     7081 ataaatatgt ctaaccttta aatcgatagt gttacgatca ccgtgttgtg ccatgtcagt
     7141 taactgttta taatcagtga tttgacttag taaataaccg agtccttgaa tcatgcctcc
     7201 acctgtaccg ataccgccta cacgacgttg tgattggccg tcgaaataat gtagtgacgt
     7261 accggtacca acatttgcaa aaatataatc tgctaagtca tggccttgct cttttaacaa
     7321 aatacctagt ccttgagatg cagcatcaaa ctctacaaaa atttgtgcag gaatgttgat
     7381 gttttcagca atgacacctg cattacctcc agttaagcat aatttttcaa tttgctgttg
     7441 gtttaaccat tccacaactt gatcaatatt tttagttaat tcagttttaa aagtacgttg
     7501 gttatcttgc tcttgaacga ttttaattag tgtaccgcca gcgtcaatgc caactttcat
     7561 aagattccca cctcattatt aatgtctatc cttaaataat agtatagtaa aatgactaaa
     7621 aaacaagtaa taatagtaat tattaacaaa tttgatgcca ttgcatttca acattgtaag
     7681 cgtatcgcaa ttaatgtttt acaaacgtgg acgttaagtt atatatatta ttttctagga
     7741 attttgaagt tgtataggat tgttagttag tgacgcaata ttaaaagtag ttcgtacgca
     7801 gtgtatttgt aagtctctga ttaaaatgat aagtaatgag gaatagtaca ttaattttga
     7861 aatttaaaaa atataaataa gtaatttatt taacttagag caaataatgg tatcgtagtg
     7921 aaataatagg taaaataata tggggattca tgcttcatat ataaaaagat aggggttaaa
     7981 tatatggcta aagaactttg ttttgaaggt atcactttaa aagcatttga tgaacaatat
     8041 cgttcagcaa ttaatgattt tgacttgaat gaaagacaac aaatatattc atctttacct
     8101 aaagaagtta ttgatgatgc aattaatgat gctgatagga ttgctaacgt agcaataaac
     8161 gataaaaatg aagtggtggg cttttttgta ttacatcgtt actatcagca tgaaggttat
     8221 gatacacctg aaaatgtcgt ttatattcgt tcattatcga ttaatgaaaa atatcaaggt
     8281 tttggatatg gcacgaaaat aatgatgtca ttgccgcaat atgttcaagg tgtatttcct
     8341 gattttaatc atctatatct agtagtagat gcggaaaatg acaatgcttg gaacctatac
     8401 gaacgtgctg gattcatgca tacagcgact aaagaagaag gaccaattgg caaagaacga
     8461 ctatattact tggacttaga ttcaaaacat gtttcatcat taaagcttga agaagaaagt
     8521 cgttcagaag tgaccaatgt acatatcatt aatttaatga ttgatggcca aaaggttggc
     8581 tttatcgcat tggagcagat tggtgaacgc atgaacattg ctgctattga agtggataaa
     8641 tcatatcgct ttaatggtat tggttcaagt gctctgcgac aattgccaac ttacttaaga
     8701 aaaaactatg acaaccttaa tgtgattacg atgattctgt ttggagagaa taatgatttt
     8761 aaaccattat gtttaaatag taatttcgtt gaaatcgaac aaactgatga ttatgtcgtt
     8821 ttcgaaaaat atttaaatta ctaacagtga ttgcgaaata tgatattgtc atttataatt
     8881 tagttttgtt actatatata aatgaattca gacgtataaa tttagattat atccttcgaa
     8941 aggaagtatt gggcaatgaa aattcaagat tatacaaaac aaatggttga tgaaaaatca
     9001 tttattgata tggcttatac attattgaat gataaaggcg aaacaatgaa cttatatgat
     9061 atcatcgatg aatttagagc gttaggtgat tatgagtacg aagaaattga aaatcgtgtt
     9121 gtacaatttt acacggattt aaacacagat ggtcgttttt taaatgttgg agaaaattta
     9181 tggggattac gtgattggta ttcggtagat gatattgaag agaaaatcgc accaactatt
     9241 caaaaattcg atattctgga tgcagatgat gaagaagatc aaaacttaaa attattgggc
     9301 gaagatgaaa tggatgacga cgatgatatt ccagctcaaa cagatgatca agaagaacta
     9361 aatgatccag aagatgagca ggttgaagaa gaaatcaatc attcggatat agtcattgaa
     9421 gaagatgaag atgaactaga cgaagacgaa gaagtgtttg aagacgaaga agacttcaac
     9481 gattaatttt ttgtttgact tttagttgaa agatgataaa attttattcg ggctccttta
     9541 aataggacac gtgtataaaa tttatacgct ccccttacag aatttgtgag agggagcgtt
     9601 tttttattta attgagtaaa tcaagaaatg ataacgcaaa aatcaaagtt gtaaatgata
     9661 tacatagtga catagcagta tggaaacggt aagtaaacag aatttaattt tgtcgaattc
     9721 gacaaataaa caacttgaat gagcttgctt taatgttatg ttttacgtaa tttttacaat
     9781 tgatgaggaa gcattccctt taataattag gaggtcaaga catgacaaaa tttatttttg
     9841 taacaggtgg cgtagtttca tcattaggga agggtattac agcatcttct ctaggtagat
     9901 tattaaaaga tagaggtcta aatgtaacaa ttcaaaaatt cgatccatac ttaaatgttg
     9961 acccaggtac aatgagtcct tatcaacatg gtgaagtatt cgtaacggat gatggtgcag
    10021 aaactgacct agacttagga cattacgaaa gatttattga tattaattta aacaagtttt
    10081 caaatgtgac agccggtaaa gtgtattcac acgtattgaa aaaagaacgt cgtggtgatt
    10141 acttaggcgg aacagttcaa gttattccgc atattacaaa tgaaattaaa gaacgtttat
    10201 tacttgcagg ggaaagtacg aatgcagacg ttgttatcac tgaaattggc ggtacaacag
    10261 gtgatattga gtcattaccg tttattgaag cgattcgtca aattcgtagc gatttaggta
    10321 gagaaaatgt tatgtatgtt cactgtacat tactgcctta tattaaagct gctggagaaa
    10381 tgaaaacgaa gccaacacaa catagtgtta aagaattacg aggcttaggt attcaaccag
    10441 acttaatcgt tgtaagaact gaatatgaaa tgacacaaga tttaaaagat aaaattgcat
    10501 tattctgtga cattaataaa gaaagtgtta ttgaatgtcg tgatgcagac tctttatacg
    10561 aaattccatt acaattaagc caacaaaata tggatgatat cgttattaaa cgtttacaat
    10621 taaacgcgaa atatgaaaca cagcttgatg aatggaaaca gttgttagat atcgttaata
    10681 atttagatgg taaaattaca attggtttag taggtaaata tgttagctta caagatgcat
    10741 atttatcagt tgttgaatca ttgaaacatg ctggatatcc ttttgccaaa gatattgaca
    10801 ttagatggat tgattcaagt gaagtaacag atgaaaatgc agccgaatac cttgcagatg
    10861 tcgacggtat tttagtacca ggtggatttg gtttccgtgc aagtgaaggt aaaattagtg
    10921 caattaagta tgctagagaa aacaatgtac cattctttgg tatttgttta ggaatgcaac
    10981 ttgcaacagt tgaattttca agaaacgtat taggccttga aggcgcacat tcagctgaat
    11041 tagacccagc aacaccatac ccaattatag atttattacc agaacaaaaa gatatcgaag
    11101 atttaggtgg tacattacgc ttaggcttat atccatgttc aattaaagaa ggcacattgg
    11161 cacaagatgt ttatggtaaa gcggaaattg aagaaagaca tcgtcatcgt tatgaattta
    11221 ataatgacta tagagaacaa ttagaagcaa atggtatggt gatttctggt acaagtccag
    11281 atggacgttt agtagaaatg gtagagattc cgacaaatga tttctttatt gcttgtcaat
    11341 tccacccaga attcttatct agaccaaatc gtccgcaccc gatttttaaa tcatttattg
    11401 aagcttcatt aaaatatcaa caaaataaat aaatttgcta ataaaaccgg tactttcatt
    11461 gttaaacatt gaaagtaccg gtttttcgta taattttaat attatgttag tgacaaggta
    11521 tgaaataaca atagtgactt ttataattct aagtctcttg tcatttcaat catttgtgta
    11581 taaatgtcat agtatacata attcaatgcc atcgcatgtg gttggacaat cttatcgtaa
    11641 tcttcagtgt agactatagg tcttggtgta gataaatcga taaaatgtac gagatgatca
    11701 gggaaatcat ctgttttagg tttgttgctt attaagacca catcgatatc taagtcgata
    11761 agtttttgaa tatctaatgc aacttgatca ttataaaatg gtgcgaataa taatacacga
    11821 tcagttgagt caatttcttt aaagtcttta atagcgtcaa gttttcggct agattttaaa
    11881 cgctcatcac tatgtagaat gaaactttcg aaaaactgta aatcatcata accttttaca
    11941 taaacataac cttcgccacc aattgcttga attaaacatt gggcggccat ttgaatttct
    12001 aaagattgtt tttctagcct attaaagata cctattagtt gtgtgtttaa gatttttgac
    12061 atctttatcc tccaatctac ttataaaata ttgtaattaa tgactacata ttatgcaacg
    12121 gcttaaattg tataaaaatg tatacgtttg catttagtat aactatcgca tttttcaaaa
    12181 aatacacatt taatctgcag tatttcaatg cattgacgct atttttttga tataattact
    12241 ttgaaaaata cgtgcgtaag cactcaagga ggaactttca tgcctttagt ttcaatgaaa
    12301 gaaatgttaa ttgatgcaaa agaaaatggt tatgcggtag gtcaatacaa tattaataac
    12361 ctagaattca ctcaagcaat tttagaagcg tcacaagaag aaaatgcacc tgtaatttta
    12421 ggtgtttctg aaggtgctgc tcgttacatg agcggtttct acacaattgt taaaatggtt
    12481 gaagggttaa tgcatgactt aaacatcact attcctgtag caatccattt agaccatggt
    12541 tcaagctttg aaaaatgtaa agaagctatc gatgctggtt tcacatcagt aatgatcgat
    12601 gcttcacaca gcccattcga agaaaacgta gcaacaacta aaaaagttgt tgaatacgct
    12661 catgaaaaag gtgtttctgt agaagctgaa ttaggtactg ttggtggaca agaagatgat
    12721 gttgtagcag acggcatcat ttatgctgat cctaaagaat gtcaagaact agttgaaaaa
    12781 actggtattg atgcattagc gccagcatta ggttcagttc atggtccata caaaggtgaa
    12841 ccaaaattag gatttaaaga aatggaagaa atcggtttat ctacaggttt accattagta
    12901 ttacacggtg gtactggtat cccgactaaa gatatccaaa aagcaattcc atttggtaca
    12961 gctaaaatta acgtaaacac tgaaaaccaa atcgcttcag caaaagcagt tcgtgacgtt
    13021 ttaaataacg acaaagaagt ttacgatcct cgtaaatact taggacctgc acgtgaagcc
    13081 atcaaagaaa cagttaaagg taaaattaaa gagttcggta cttctaaccg cgctaaataa
    13141 ttaatattta gtctttaagt tattaataac gtagggatat taattttaaa agaagcagac
    13201 aaaatggtgt ttgcttcttt tttatgtcgt ataagtaata aataaaacag tttgatttta
    13261 aaatgaaagc gtaaaaatgg taaaatatat caaaattgat tgtgatataa ttataaggaa
    13321 aatgagcaat ttatgaaaaa agtttacgtg aaaaccgtag aattaaaact aaataattgt
    13381 caaaacaacg tcaatattta gttgaataat cagactttag accatggtca agtggggaag
    13441 aacagcatat attagtaaag gtgaatgatt tgttattaat caatcgaaaa tagaaagaca
    13501 agattttaac gattaaaata aactatttta caaataaagt aaaattaatt tataatgcta
    13561 ataatgcaaa aaattaaaaa gtaatggaca aaggagataa atgatatggc tcaagaggta
    13621 ataaaaataa gaggtggacg cacactaaat ggggaagtta atatcagtgg cgcgaaaaac
    13681 agtgctgtag ctatcattcc tgcaacatta ttagctcaag gacatgtgaa attagaaggg
    13741 ttaccgcaaa tctctgatgt taaaacttta gtgagtttat tagaagattt aaatattaag
    13801 gcatcactaa atggtacgga attagaagtt gatacaactg aaatacaaaa tgctgcatta
    13861 ccaaataata aagttgaatc attacgtgct tcatactata tgatgggtgc catgttaggt
    13921 agatttaaaa aatgtgtgat tggtttaccg ggaggatgtc cacttgggcc acgtccaatt
    13981 gatcaacata ttaaaggatt taaagcttta ggtgctgaaa ttgatgaatc aagtacgaca
    14041 tcaatgaaaa tcgaagctaa agagttaaaa ggtgcacata tttttctaga tatggttagc
    14101 gtaggtgcaa caattaacat tatgctagct gcagtttatg caacaggaca aactgtaatt
    14161 gaaaatgctg ctaaagaacc ggaagtagtt gatgttgcta acttcttaac aagtatggga
    14221 gctaatatta agggagcggg tacatcaaca attaaaatca atggcgtcaa agaattacat
    14281 ggttctgagt atcaagttat acctgataga attgaagcag gcacatatat gtgtatcgct
    14341 gcagcatgtg gagaaaatgt tatattaaat aatattgttc cgaaacatgt agaaacatta
    14401 actgctaaat ttagtgaatt aggtgtgaat gttgatgtaa gagacgaaag aattcgcatc
    14461 aataataatg cgccatatca atttgttgat attaaaacac tagtatatcc aggttttgct
    14521 actgatttgc aacagcctat tacaccatta ttatttatgg caaatggtcc ttcatttgta
    14581 actgatacga tttatccgga gcgttttaaa catgttgaag aattaaagcg catgggtgca
    14641 aatattgaag ttgacgaagg cacagcaacg ataaaaccat caacattaca tggtgctgaa
    14701 gtttatgcta gcgatttaag agcaggagct tgtttaatta ttgctggttt aattgctgag
    14761 ggtgtaacta caatttacaa tgttaaacat atttatagag gttatacaga tattgtagaa
    14821 cacttaaaag ctttaggtgc agatatttgg acggaaactg tatagattta tcttgttttg
    14881 tggtataata attatactga ttaaaaaatg gtgttcgttt acattctaaa tttattgatg
    14941 agaggtgaag atgatggaag tatgtccgta tctcgaagaa acttttaaaa tacttggtag
    15001 aagttggaat ggattaatca ttaattatct ctcaagatgt aatgactgtt cagcacactt
    15061 ttccgatatg aaaagagatt tgaaaacaat aacaccacgt gctttaagtc ttaagttgtc
    15121 agagcttgca caatgggagt tagttgagaa gcaaatcatt tctacgagtc cagtacaaat
    15181 tatttatgtg ctaactgaaa aaggtaaagc gttagcagag gctttacatc caattgaagc
    15241 atgggcgcaa tcatatgtcg atttaacaga tcaacgtact gctaaataat tacatgacgt
    15301 ttaaacatag cattgattat aactatttct aagtcttcgc attatttgcg atgatgtggg
    15361 aatagttatt tttatttaaa aatataaaaa aatagatgca gcaaaatttt aaagcatttt
    15421 attttgaaca tattaaaagg gagcgtatca taatggaatg taatgtttat atcgtatgca
    15481 ttacggataa ataatatata aatcattctt gaggagtgaa agaataatga gagactacac
    15541 aaagcaatac attaatggcg aatgggtaga aagtaatagt aatgaaacga tagaagttat
    15601 aaatccagca accgaagaag taatcgggaa agttgctaaa ggtaataaag ctgatgttga
    15661 taaagccgtc gaggcggcag acgatgttta tttagagttc cgtcatacat ctgtgaaaga
    15721 aagacaagcg ttattagata aaattgtaaa agaatatgaa aacagaaaag acgatattgt
    15781 acaagctatt acggatgaat taggtgctcc tttatcatta tctgagcgtg tccattatca
    15841 aatgggacta aaccattttg ttgcagcgag agacgcatta gataactacg aatttgaaga
    15901 acgccgcgga gatgatttag ttgttaaaga agcaatcggt gtatctggat taattacacc
    15961 gtggaacttc cctacaaacc aaacatcatt aaaattagca gcagcatttg cggctggtag
    16021 tccagttgta cttaaaccat ctgaagaaac accatttgca gctgttattt tagctgagat
    16081 ttttgataaa gtcggtgttc ctaaaggtgt atttaacctt gttaatggtg atggtgctgg
    16141 tgttgggaat cctttatctg aacatcctaa agtacgcatg atgtcattta caggatcagg
    16201 ccctactggt tctaaaatta tggaaaaagc cgctaaagat tttaaaaagg tatcattaga
    16261 gcttggtggc aaatcaccat atatcgtcct agatgacgta gatattaaag aagcggctaa
    16321 agcaacaaca ggcaaagttg ttaataatac tggtcaagta tgtacagctg gtacacgtgt
    16381 tttagtgcct aacaaaatta aagatgcatt cttagctgaa ttaaaagaac aatttagcca
    16441 agtgcgtgtc ggtaatccaa gagaagatgg tacacaagta ggccctatca ttagtaaaaa
    16501 acaatttgat caagtacaaa attatattaa taaaggtatt gaagaaggtg ctgaattatt
    16561 ttatggtggt cctggtaaac cagaaggact tgaaaaagga tactttgcac gtccgacaat
    16621 ttttattaat gtagataatc aaatgacgat agcacaagaa gaaatttttg ggccagtaat
    16681 gtcagttatc acttataacg atttagatga agcgattcaa attgcaaatg atacaaaata
    16741 tggtttggca ggatatgtta ttggtaagga caaagaaaca ttgcataaag tagctcgttc
    16801 tattgaagca ggtacagtag aaataaacga agcaggtaga aagccagatt taccatttgg
    16861 tggctataaa caatctggtt taggtcgtga atggggcgat tatggtattg aagagttctt
    16921 agaagtgaaa tctatagctg gatattttaa ataaatttga tttttgaatt aaaaatcgca
    16981 ataaaacagt gcacatgact aattaagttt tgtgtactgt tttaattttg caatttttat
    17041 aaatagattt tgtaattaaa ataaaaattt gctatagtta ttcatgtatt taaaaggttg
    17101 gggattagca taatgggatt gtgctagcac agttatttat gcattgtcat gcctatctat
    17161 tacttactaa ctaaaaaata atgaaatggg tgtaaactat atgcctgaaa gagaacgtac
    17221 atctcctcag tatgaatcat tccacgaatt gtacaagaac tatactacca aggaactcac
    17281 tcaaaaagct aaaactctta agttgacgaa ctatagtaaa ttaaataaaa aagaacttgt
    17341 tctagctatt atggaagcac aaatggaaaa agatggtaac tattatatgg aaggtatctt
    17401 agatgatata caaccagatg gttatggttt tttaagaaca gtgaactatt ctaaagggga
    17461 aaaagatatt tatatatctg ctagccaaat tcgtcgtttt gaaattaaac gtggggataa
    17521 agtaactggg aaagttagaa aacctaaaga taacgaaaaa tattatggct tattacaagt
    17581 tgactttgtc aatgaccata acgcagaaga agtgaagaaa cgtccgcatt tccaagcttt
    17641 gacaccactt tatccagatg agcgtattaa attagagaca gaaacacaaa attattcaac
    17701 gcgcatcatg gatttagtaa caccgattgg tttaggtcaa cgtggtttaa tagtggcgcc
    17761 acctaaagca ggtaaaacat cgttattaaa agaaatagcg aatgcaatca gtacgaacaa
    17821 accagatgca aagctattta ttttgttagt tggcgagcgt cctgaagagg taacagattt
    17881 agaacgctca gtagaagctg ctgaagtcgt tcattcaacg tttgacgaac caccagaaca
    17941 ccatgttaaa gtagctgaat tattacttga acgtgcaaag cgtttagtag aaattgggga
    18001 agatgtcatt attttaatgg attctataac gagattagca cgcgcttata acttagttat
    18061 tccaccaagt ggtcgtacat tatcaggtgg tttagatcct gcatctttac acaaaccaaa
    18121 agcattcttc ggtgcagcga gaaatattga agcgggtgga agtttaacaa tacttgcaac
    18181 tgcattagtt gatacgggtt cacgtatgga cgatatgatt tacgaagaat ttaaaggaac
    18241 aggtaacatg gagttacatt tagatcgtaa attgtctgaa cgtcgtatct tccctgcaat
    18301 tgatattggc agaagttcaa cgcgtaaaga agaattgttg ataagtaaat ctgaattaga
    18361 cacattatgg caattaagaa atctattcac tgactcaact gactttactg aaagatttat
    18421 tcgcaaactt aaaaggtcta agaataatga agatttcttc aagcagctac aaaagtctgc
    18481 agaagaaagt actaaaacgg gtcgacctat aatttaataa acattatata ggggcttgcg
    18541 ttttgaatta attaccttta taattacaca gtattgggta aaaactcaca aataactctg
    18601 ttccagatgg ttcagggcaa aggagctgaa aataatgaaa caaggaattc atcctgagta
    18661 tcaccaagta atctttttag atactactac aaacttcaaa ttcttaagcg gttctacaaa
    18721 aacatcttca gaaatgatgg agtgggaaga tggaaaagaa tacccagtta ttcgtttaga
    18781 tatttcatct gattcacatc cattctacac tggacgtcaa aaattcgctg ctgcggatgg
    18841 acgtgtggaa cgtttcaaca aaaagtttgg tcttaaatca aacaactaat atagtaatcg
    18901 ttaagctgtc tgcatcctat aatggaattg tagacagctt ttttatttat aagcgtagta
    18961 agggtatata cttacgactg tgttcaaaga agtgactcaa taccatgttg ttaatacata
    19021 atgataaatt tcccccttta ttaaagtttg tattgagtag aaacatcgca cgaataagaa
    19081 gcaacaaatt agaatagctg tatataaata tgcaagacaa atttataaaa taattgttca
    19141 tgtacaatat cgatataatt atgaaaaaga catttatgaa tatttgaaat atgatataat
    19201 aaagcgatta tgttttgaaa aaggtggaac tctcaatgta tgaaacttac cattccggtt
    19261 ggatagaatg tattacaggt agtatgttta gcggtaaatc cgaagaacta ataagacgtc
    19321 taagaagggg tatatacgct aaacaaaaag tcgttgtatt taaaccggcg attgatgatc
    19381 gatatcataa agaaaaagtg gtctctcata atggtaatgc cattgaagcg attaatattt
    19441 ctaaagcaag tgaaattatg acgcacgact taactaatgt tgatgttata ggaatagacg
    19501 aagtgcaatt ttttgacgat gaaattgtaa gtatagtaga aaaactatca gcagatggac
    19561 accgtgttat tgttgctgga ttagatatgg attttagggg cgaaccgttc gaaccaatgc
    19621 ctaaattgat ggctgtcagt gaacaagtaa cgaaacttca agcagtttgt gctgtttgtg
    19681 gttcttcatc tagtagaacg caacgattaa taaatggtaa accagctaaa atcgatgatc
    19741 cgattatctt agtaggtgcc aatgaaagtt atgagccacg ttgtcgcgcg caccatatcg
    19801 tagcacctag tgataataat aaggaggaat tataagtgtt tgatcaatta gatattgtag
    19861 aagaaagata cgaacagtta aatgaactgt taagtgaccc agatgttgta aatgattcag
    19921 ataaattacg taaatattct aaagagcaag ctgatttaca aaaaactgta gatgtttatc
    19981 gtaactataa agctaaaaaa gaagaattag ctgatattga agaaatgtta agtgagactg
    20041 atgataaaga agaagtagaa atgttaaaag aggagagtaa tggtattaaa gctgaacttc
    20101 caaatcttga agaagagctt aaaatattat tgattcctaa agatcctaat gatgacaaag
    20161 acgttattgt agaaataaga gcagcagcag gtggtgatga ggctgcgatt tttgctggtg
    20221 atttaatgcg tatgtattca aagtatgctg aatcacaagg attcaaaact gaaatagtag
    20281 aagcgtctga aagtgaccat ggtggttaca aagaaattag tttctcagtt tctggtaatg
    20341 gcgcgtatag taaattgaaa tttgaaaatg gtgcgcaccg cgttcaacgt gtgcctgaaa
    20401 cagaatcagg tggacgtatt catacttcaa cagctacagt ggcagtttta ccagaagttg
    20461 aagatgtaga aattgaaatt agaaatgaag atttaaaaat cgacacgtat cgttcaagtg
    20521 gtgcaggtgg tcagcacgta aacacaactg actctgcagt acgtattacc catttaccaa
    20581 ctggtgtcat tgcaacatct tctgagaagt ctcaaattca aaaccgtgaa aaagcaatga
    20641 aagtgttaaa agcacgttta tacgatatga aagttcaaga agaacaacaa aagtatgcgt
    20701 cacaacgtaa atcagcagtc ggtactggtg atcgttcaga acgtattcga acttataatt
    20761 atccacaaag ccgtgtaaca gaccatcgta taggtctaac gcttcaaaaa ttagggcaaa
    20821 ttatggaagg ccatttagaa gaaattatag atgcactgac tttatcagag cagacagata
    20881 aattgaaaga acttaataat ggtgaattat aaagaaaagt tagatgaagc aattcattta
    20941 acacaacaaa aagggtttga acaaacacga gctgaatggt taatgttaga tgtatttcaa
    21001 tggacgcgta cggactttgt agtccacatg catgatgata tgccgaaagc gatgattatg
    21061 aagttcgact tagcattaca acgtatgtta ttaggagagc ctatacagta tatagttggc
    21121 tttgcctcat tttatggtag aacgtttgat gtaaactcaa attgtttgat accaagacct
    21181 gaaactgaag aagtaatgtt gcatttctta caacagttag aagatgatgc aacaatcgta
    21241 gatatcggaa cgggtagtgg tgtacttgca attactttga aatgtgaaaa gccggattta
    21301 aatgttattg ctactgatat ttcacttgaa gcaatgaata tggctcgtaa taatgctgag
    21361 aagcatcaat cacaaataca atttttaaca ggggatgcat taaagccctt aattaatgaa
    21421 ggtatcaagt tgaacggctt gatatctaat ccaccatata tagatgaaaa agatatggtt
    21481 acgatgtctc caacggttac gagattcgaa ccacatcagg cattgtttgc agataaccat
    21541 ggatatgcta tttatgaatc aatcattgaa gatttacctc acgttatgga aaaaggcagc
    21601 ccagttgttt ttgaaattgg ttacaatcaa ggtgaggcac ttaaatcaat aattttaaat
    21661 aaatttcctg acaaaaaaat cgacattatt aaagatataa atggccacga tcgaatcgtc
    21721 tcatttaaat ggtaattaga agttatgcct ttgctatgat tagttaagtg catagctttt
    21781 tgctttatat tatgataaat aagaaaggcg tgattaagtt ggatactaaa atttgggatg
    21841 ttagagaata taatgaagat ttacagcaat atcctaaaat taatgaaata aaagacattg
    21901 ttttaaacgg tggtttaata ggtttaccaa ctgaaacagt ttatggactt gcagcaaatg
    21961 cgacagatga agaagctgta gctaaaatat atgaagctaa aggccgtcca tctgacaatc
    22021 cgcttattgt tcatatacac agtaaaggtc aattaaaaga ttttacatat actttggatc
    22081 cacgcgtaga aaagttaatg caggcattct ggccgggccc tatttcgttt atattgccgt
    22141 taaagctagg ctatctatgt cgaaaagttt ctggaggttt atcatcagtt gctgttagaa
    22201 tgccaagcca ttctgtaggt agacaattat tacaaatcat aaatgaacct ctagctgctc
    22261 caagtgctaa tttaagtggt agaccttcac caacaacttt caatcatgta tatcaagatt
    22321 tgaatggccg tatcgatggt attgttcaag ctgaacaaag tgaagaagga ttagaaagta
    22381 cggttttaga ttgcacatct tttccttata aaattgcaag acctggttct ataacagcag
    22441 caatgattac agaaatactt ccgaatagta tcgcccatgc tgattataat gatactgaac
    22501 agccaattgc accaggtatg aagtataagc attactcacc caatacacca cttacaatta
    22561 ttacagatat tgagagcaaa attggaaatg acggtaaaga ttggtcttct atagctttta
    22621 ttgtgccgag taataaggtg gcgtttatac caagtgaagc gcaattcatt caattatgtc
    22681 aggatgacaa tgatgttaaa caagcaagtc ataatcttta tgatgtgtta cattcacttg
    22741 atgaaaatga aaatatttca gcggcgtata tatacggctt tgagctgaat gataatacag
    22801 aagcaattat gaatcgcatg ttaaaagctg caggtaatca cattattaaa ggatgtgaac
    22861 tatgaagatt ttattcgttt gtacaggtaa cacatgtcgt agcccattag cggaaagtat
    22921 tgcaaaagag gttatgccaa atcatcaatt tgaatcaaga ggtatattcg ctgtgaacaa
    22981 tcaaggtgtt tcgaattatg ttgaagactt agttgaagaa catcatttag ctgaaacgac
    23041 cttatcgcaa caatttactg aagcagattt gaaagcagat attattttga cgatgtcgta
    23101 ttcgcacaaa gaattaatag aggcacactt tggtttgcaa aatcatgttt tcacattgca
    23161 tgaatatgta aaagaagcag gagaagttat agatccatac ggtggaacaa aagaaatgta
    23221 tgtacatacc tatgaagaac ttgtaagttt aattttaaaa ttaaaagata ttatttgcta
    23281 gtaggaattc tgtataatta gtatatgtta acagttaaat aagttataat agaggagtgt
    23341 agcactttgc tatattcctt ttttattttt aggaagggtg ggcttttatg aaagatttga
    23401 caatgttatt agacgaatta aaagatatgt ccttttttaa taaaggagat atatgtttaa
    23461 ttggatgttc aacatcggaa gtcattggcg aaaaaatagg gactgtcggt tcgatggaag
    23521 ttgcagaaac tatttttaat gcattagatg ttgtgagtaa agagacaggc gttacttttg
    23581 cttttcaagg atgtgaacat atcaacagag cgataacaat agaaaaatct cagtataatc
    23641 cattaacaat ggaagaagtt tccgtcgttc cagatgtaca tgctggaggg agtttggcaa
    23701 catacgcttt tcagcatatg aaggatccga tcgtggtcga acatattact gttccttgtg
    23761 gaattgatat tggtcaaacg ttaataggca tgcatattaa gcatgtatgt gtacctgtaa
    23821 ggacctcagt aaagcaagta ggacaggcaa ttgtaactat tgccacatct aggccgaaaa
    23881 agattggtgg cgaacgtgct aaataccaat aagttttttt gaaagaaggg gagattttat
    23941 gtcttatatc accaagcaag ataaagttat cgcagaagca atcgagagag aatttcagag
    24001 acaaaatagc aacatagagt taatcgcatc ggaaaatttt gtatcggaag cggttatgga
    24061 agcacaaggt tcagtgttga ctaataagta tgctgaaggc tatccaggac gccgatatta
    24121 tggtggctgt gagtttgtag atgttactga aagcatcgca attgatcgtg ctaaagcatt
    24181 gtttggagct gaacatgtca atgttcaacc acattcaggt tcacaagcga acatggctgt
    24241 ttacttagtt gcattaggaa tgggcgacac agttttaggt atgaatttga gtcatggtgg
    24301 tcacttgaca catggagcgc ctgttaattt tagtggtaaa ttctacaatt tcgttgaata
    24361 tggagtagat aaagacacag aacgaatcaa ttatgatgaa gttcgtaaat tagcgttaga
    24421 gcataagcct aagcttattg tggcaggagc atcagcatat tcaagaacaa ttgacttcaa
    24481 aaagtttaaa gaaatcgcag atgaagtaaa cgctaagtta atggtagaca tggcacatat
    24541 tgcaggatta gtagcggcag gtttacatcc aaatccagta gaatatgctg attttgtaac
    24601 aactacaaca cacaaaacat tacgcggacc acgtggtggt atgattttat gtaaggaaga
    24661 atataaaaaa gacatagata aaacaatttt ccctggtatt caaggtggac ctcttgagca
    24721 tgttattgca gcaaaagcag ttgcttttgg agaagcgtta gaaaataatt tcaaaacgta
    24781 tcaacaacaa gtggttaaaa acgcaaaagt tcttgcagaa gcattaatta atgaaggatt
    24841 tagaattgtt tctggcggta cagataatca cttagtagct gttgatgtaa aagggtctat
    24901 aggacttact ggtaaagaag ctgaagagac tttagattca gttggtatca catgtaacaa
    24961 aaataccatt ccgttcgatc aagaaaaacc ttttgtaacg agtggtatac gtttaggtac
    25021 acctgctgca acaacgcgtg gatttgatga aaaagctttt gaggaagttg caaaaatcat
    25081 cagtttagca ttgaaaaata gtaaagatga agaaaaatta caacaagcta aagaacgcgt
    25141 tgcgaaatta acagctgaat atcctctata tcaataaaca tacacaaaga taggagtaat
    25201 ttttatgagt aaagtacacg ttttcgatca tccattaatt caacacaaac taagctatat
    25261 tagagatgta aatacaggta ctaaagaatt tagagaactt gtagacgaag ttggtatgtt
    25321 gatggcatat gaagtaacta gagacttaga gcttcaggat gttgatattg aaacgcctgt
    25381 aactaaaatg acggctaaac gattagctgg taaaaaatta gctatcgttc caattttaag
    25441 agctggttta ggtatgactg atggtatttt aagtttagta cctgcagcca gagttggaca
    25501 tatcggttta tatcgagatc ccgaaacatt aaaggctgta gagtattttg ctaagttacc
    25561 tcaagatatt actgaaagac aaatcattgt tgttgatcct atgttagcaa caggcgcatc
    25621 agcaattgaa gctattactt cattgaagaa acgtggcgct aaaaatattc gcttcatgtg
    25681 tttaattgca gcaccagaag gcgtagaaaa gatgcatgaa gcacatccag atgtagatat
    25741 ttatattgct gcacttgatg aaaagctaaa tgacaaagca tatatcacac cagggttagg
    25801 cgacgctgga gatagattgt ttggtactaa atagaagaat cggagataag tagtttgatg
    25861 aaaaagatta tgaccatatt tggaacaaga cccgaagcca taaagatggc tcctttagtt
    25921 aaagctttgg aacaagaaaa aatgctcgag ccaattgttg ttgttacagc acagcataga
    25981 gaaatgcttg attcagtatt aagtaccttt gaaatcaaac caaaatacga tttgaatatt
    26041 atgaaatcag gacagacact ttctgagata acttctaaat caatcactca attagaacaa
    26101 gtgattcaat tagaaaaacc agacatggta cttgtacatg gtgatacgat gacgacattt
    26161 gcaggtggtt tagcggcatt ttataaccaa gtgccgattg gacacgttga agcagggctt
    26221 agaagttatg ataaatattc accttttcca gaagaggtga atagacaatt agttggtgta
    26281 ttagctgact tacattttgc acctactaaa aatgctgcat cgcatttgtt aagtgaaggg
    26341 aaatattcag aaagtgttgt tgtcactggt aatactgcga ttgatgctat gaaatataca
    26401 gttgatgaca attataaatc taacatcatg gataaatacc atgacaaaaa attcattctt
    26461 atgacagcac accgacgaga aaatatcggg aaacccatgg aaaatatatt taaagcggtt
    26521 agacgcttaa tagatgagta tactgattta gcactggtat atccaatgca taaaaacccg
    26581 aaagttcgag aggtggctca aaagatttta ggtagtcatg acagaattga attgattgaa
    26641 cctttggacg tagtagattt ccataatttt gctaaaaaat cttattttat tttgacagat
    26701 tcaggtggaa ttcaggaaga agcgccatca tttaataagc cagttttagt tttgcgaagt
    26761 gttacagaac gtcctgaagg tgttgaagct ggcacattaa aagtcattgg aacaaataag
    26821 caaaatgtgt atcaagctgc aaaagaatta atcgatgatg agagattata tcatcaaatg
    26881 tctgaagcat ctaatcctta cggtgatgga tttgcttcag aaagaatagt taatcatatt
    26941 aagtattatt tgaatttaat cactgaaaaa ccaagcgatt tctgaggaaa atagactttt
    27001 tgtctaattg tgtctaaatt gtgtctaact atgcttattt ttttacaaca atttgaatag
    27061 aattgacacc acaaatcgcc tatattatca tttagtttgt atgagtggaa cggttttcat
    27121 gattgatatc agtcacacgg aggcatataa aatgagtcgt ttctacaaca tttttaaaca
    27181 gttcattcaa tattattttt atctaataat aatattggga ggattatacc tttatacaca
    27241 ccatgcattt atcttaggat taatcattgg tgttactggt tctgcaatca acacatgtat
    27301 ttttgaatgc tatttagcta aagctaaaag accagacact atgcatattt caactggaaa
    27361 tatgtggcga tacttagttg caattattgc ctgtatgatt tggtacctta ataaagcgca
    27421 tgtaagtatc atcggtataa ttattggttt aatgatttca tatgttgtag ttatcatacg
    27481 tcctttacta aaggtgagca aataaattaa gaaagaggtg agattatgga tcacaaatcc
    27541 ccgctcgtga gttggaattt attcggtttt gatatcgttt tcaatttatc aagtatattg
    27601 atgatacttg ttacggcgtt tcttgttttt ctacttgcta tcatttgtac gcgtaatttg
    27661 aaaaaaagac caactggcaa acaaaatttc gttgaatgga tttttgattt cgtgagggga
    27721 atcattgaag gtaacatggc ttggaaaaaa ggtggtcaat tccacttctt agcagtaacg
    27781 ctgattctgt acatttttat agctaatatg ttaggtcttc cgttttctat agtaacgaaa
    27841 gatcacacat tgtggtggaa atcaccgaca gctgatgcaa cagtgacttt aacgttgtct
    27901 acaacgataa tactgttaac tcacttttat ggaattaaaa tgcgtggtac gaaacaatat
    27961 cttaaaggtt atgtacagcc gttttggcca ttggcaatta ttaatgtttt tgaagagttc
    28021 acttcaacat taacgcttgg tctgcgtttg tacggtaaca tatttgcagg tgagatacta
    28081 ttaacattac ttgctggctt attctttaac gaaccagcat ggggttggat tattagtatc
    28141 ccaggattaa ttgtttggca agcattttca atatttgtag gaacaatcca agcatatatc
    28201 tttattatgc tttcgatggt ttatatgtca cataaagtgg cagatgaaca ctaaaaattt
    28261 caataattat atacaatcac aggaggaaat taaattatga atttaatcgc agcagcaatc
    28321 gcaattggtt tatcagcatt aggagcaggt atcggtaacg gtttaatcgt ttcaagaaca
    28381 gttgaaggtg tagcacgtca accagaagca cgtggtcaat taatgggtat catgttcatt
    28441 ggtgtaggtt tagttgaggc attacctatc atcggtgtag taattgcatt catgacattt
    28501 gctggataat taacagataa aagaggtcgg gacaaagcgc ataggacata attcatgatg
    28561 catatatagt aatatctttg aactttatta aatagttgag atatgaacgc accatgccta
    28621 tcgcataaat tcagtaggtc ctaacctcgt cgtttttttc tatataacac tagcgattat
    28681 tttaatgaaa ggagtgtcat gaacccgtga ctgaaacagc taacttattc gttcttggtg
    28741 cagctggagg cgttgagtgg ggtactgtga ttgtacaggt cctaactttc atcgtgttac
    28801 ttgcgttact taaaaagttc gcatggggtc cattgaaaga tgtaatggat aaacgtgaaa
    28861 gagatattaa cagagatatc gatgacgcag aacaagctaa gttaaatgca cagaaacttg
    28921 aagaagaaaa taaacaaaaa cttaaagaaa cacaagaaga agttcaaaag attttagaag
    28981 atgctaaggt tcaagcacgt caacagcaag aacaaattat tcatgaagca aacgtacgtg
    29041 caaacggtat gattgaaaca gcacaaagtg aaatcaatag ccaaaaagaa cgtgccattg
    29101 cagatattaa taatcaagta tctgaactat cagtgttaat tgcttctaaa gttcttagaa
    29161 aagaaatttc tgaacaagac caaaaagcat tggttgacaa gtatctaaaa gaggcaggcg
    29221 ataaataatg gtaaaagtag ctaacaagta tgctaaagca ttatttgacg tgtcattaga
    29281 tacaaataat ttagagacta ttaatgaaga attaacagtt ataaatgaag cagtaaaaga
    29341 taaaattgag caattgagaa tggttgacag taatccaact caaacagcag aacaacgtcg
    29401 tgagttgatt aatggtgtat ttactgacat taatccatac attaaaaata tgatgtacgt
    29461 gttagcagat aatagacata tctcattaat agctgacgta ttcaaggcgt tccaaagctt
    29521 atataacgga cactacaatc aagattttgc aacaattgag tcaacatatg aattgagtca
    29581 agaagagtta gataagattg tcaaactagt aactcaacaa acgaagttat ctaaagttat
    29641 tgtagataca aaaattaatc cagatttaat tggtggattt agagttaaag tcggcacaac
    29701 tgtattagat ggtagtgtta gaaatgatct tgtccaatta caaagaaaat ttagaagagt
    29761 taattaatta taaagaggag tgacatagat ggccataaaa gctgaagaaa tcagtgcatt
    29821 acttcgctca caaattgaaa attatgagtc agaaatgtcc gtaactgatg taggtactgt
    29881 attacaaatt ggtgatggta ttgcattaat tcacggatta aatgacgtta tggctggtga
    29941 gctagtagaa ttccataacg gcgtacttgg tttagcccaa aaccttgaag agtcaaacgt
    30001 gggtgtggtt attttaggac catacacagg tattactgaa ggtgacgaag ttaaacgtac
    30061 tggtcgtatc atggaagtac cagtaggtga agaactaatc ggaagagttg ttaatccatt
    30121 aggacaacct attgatggac aaggaccgat taacacaact aaaacacgtc cagtagagaa
    30181 aaaagctact ggtgtaatgg atcgtaaatc agtagatgag ccattacaaa caggtatcaa
    30241 agcaattgat gctttagtac caattggtag aggtcaacgt gagttaatca tcggtgaccg
    30301 tcaaacaggt aaaacaacaa ttgcaattga cacaattttg aaccaaaaag atcaaggtac
    30361 gatttgtatc tatgttgcta ttggtcaaaa agattcaaca gtaagagcaa atgttgaaaa
    30421 gttaagacaa gcaggcgctt tagactacac tattgttgta gcagcatcag cttctgaacc
    30481 ttctccatta ttatatattg caccatattc aggtgtaaca atgggtgaag aattcatgtt
    30541 taacggtaaa catgttttaa tcgtttatga tgatttaact aaacaagcag cagcttatcg
    30601 tgagttatca ttattattac gtagacctcc aggtcgtgaa gcatacccag gtgacgtatt
    30661 ctacttacat agtagattat tagaaagagc agcaaaatta aacgatgact taggtggcgg
    30721 ttcaattact gcattaccaa ttatcgaaac acaagctggt gatatttcag cttatgtacc
    30781 aacaaacgtt atttcaatta cagatggaca aatcttctta caatctgatt tattcttctc
    30841 aggtgtaaga ccagcgatta atgccggaca atctgtatct cgtgttggtg gatctgcaca
    30901 aattaaagca atgaagaaag ttgctggtac gttacgtctt gacttagcgt catacagaga
    30961 acttgaatca tttgcacaat tcggttcaga ccttgatgaa tttactgcaa gtaaattaga
    31021 acgtggtaaa cgtactgttg aagtcttaaa acaagatcaa aacaaaccat taccagtcga
    31081 acaccaagtg ttgattattt atgcattaac aaaaggatat ttagatgata ttccagttgt
    31141 agatatcaca cgttttgaag acgagttaaa ccactgggca gaatcaaatg ctactgaact
    31201 gttaaatgaa atcagagaaa ctggtggctt accagatgct gagaagtttg acacagcaat
    31261 taacgaattc aaaaaaagct ttagcaaatc tgaataataa acaagtttag tataaggtgg
    31321 tgagatagtg gcttctctta aagaaataga tactcgaata aaatcaacca aaaaaatgaa
    31381 gcagattacg aaagcgatga acatggtatc aagttcaaaa cttcgtagag ctgaaaaaaa
    31441 tacaaaacaa ttcacaccat atatggataa aatgcaagat gcaattactg cagttgcagg
    31501 ggcaagtagc aatacaaacc atccaatgct aagacctcgt aaaattacta gaagtggata
    31561 tttagttatc acgagtgata aaggtttagc aggtgcatat agtgcaaacg tgcttaaaaa
    31621 attgattact gatattgaag cgaaacatca agatagtagc gaatacagta ttgtagtttt
    31681 agggcaacaa ggtgttgatt tcctaaaaaa tagaggttat gacattgagt attctcaagt
    31741 agacgtacct gatcaacctt ctttcaaatc tgttcaagca ctagctaacc atgctataga
    31801 cttatacagt gaagaagaaa ttgatgaatt aaatatatac tatagtcatt atgtcagcgt
    31861 tcttgaaaac aagcctacat ctagacaagt attaccatta tctcaagagg attctagtaa
    31921 ggggcatggt catttgtctt cttatgaatt tgagccagat aaagaatcta tcttaagtgt
    31981 aatcttgcct caatatgttg agagtttgat ttacggaaca atattagacg caaaagcaag
    32041 tgagcatgca acacgtatga ctgcgatgaa aaatgccact gataatgcaa ctgaacttat
    32101 tgatgactta tcattagaat ataacagagc gagacaagca gaaattacgc aacaaattac
    32161 tgaaattgtt ggtggttccg cagcgcttga ataatattta aaggaggaaa atagcatggg
    32221 aattggccgt gtaactcaag ttatgggtcc tgtaattgat gttcgatttg aacataacga
    32281 agttcctaaa attaataacg ccttggttat tgatgtgcct aaagaagaag gtacaataca
    32341 actaacatta gaagttgcgc tgcaattagg tgacgacgtt gttcgtacaa ttgcgatgga
    32401 ttcaactgat ggtgtccaaa gaggcatgga tgtaaaagat acaggcaaag aaattagtgt
    32461 acctgttggt gacgaaacat taggtcgtgt atttaatgta ctaggtgaaa caattgacct
    32521 taaagaagaa attagtgatt ctgttcgccg cgatcctatc catcgtcaag caccagcatt
    32581 cgatgaactt tcaacagaag ttcaaatttt agaaacaggt attaaagtag tagatttact
    32641 agcaccttat attaaaggtg gtaaaatcgg attgttcggt ggtgccggtg taggtaaaac
    32701 agtattaatc caagaattaa ttaacaacat cgctcaagag cacggtggta tttctgtatt
    32761 cgccggtgta ggtgaacgta ctcgtgaagg taacgattta tacttcgaaa tgagtgacag
    32821 tggtgtaatt aagaaaacag ccatggtatt cgggcaaatg aatgagccac ctggtgcacg
    32881 tatgcgtgtt gcattatctg gtttaacaat ggctgaatat ttccgtgacg aacaaggtca
    32941 agacgtatta ttattcatcg ataacatttt cagatttaca caagctggtt ctgaggtatc
    33001 tgcattatta ggtcgtatgc cttctgcagt aggttaccaa ccaacacttg ctactgaaat
    33061 gggacaatta caagaacgta ttacgtctac aacaaaagga tcagttactt ctattcaagc
    33121 ggtattcgta cctgccgatg actatactga cccagcgcct gcgacagcgt ttgcccattt
    33181 agatgcaact acaaacttag aacgtaaatt aactgaaatg ggtatttatc cagccgtgga
    33241 tccattagcg tctacatcaa gagcattgga accatcaatt gtaggtcaag aacattatga
    33301 agtagctcgt gatgtacaat caacacttca aaaataccgt gaattacaag atatcattgc
    33361 tatcttaggt atggacgaat tatctgatga agataaacaa acagttgaac gcgcacgtag
    33421 aattcaattc ttcttatctc aaaacttcca cgtagcggaa caatttactg gtcaaaaagg
    33481 ttcttatgta cctgttaaga caacagttgc aaactttaaa gatatcttag atggtaaata
    33541 tgaccatatt ccagaagatg cattccgttt agttggtagc atggatgatg ttattgcaaa
    33601 agctaaagat atgggtgttg aagtataaca attaggagga atggataatg aatacattaa
    33661 acctagatat tgtcactcct aatggttctg tttacaatcg tgataatgtt gaactcgttg
    33721 ttatgcaaac aacagctggt gagataggtg tcatgagtgg acatattcca actgtagctg
    33781 ctttaaaaac aggctttgta aaagtgaaat ttcacgatgg aactgaatat attgctgtaa
    33841 gcgatggctt tgttgaagtt agaaaagata aagtttcaat cattgttcag actgcagaaa
    33901 ctgcaagaga aattgatgtt gaaagagcta aattagccaa agcaagagca gagtctcact
    33961 tggaaaatga tgacgacaat actgatattc atagagccga aagagcttta gagagagcaa
    34021 ataaccgttt gcgtgtggct gaattaaaat agtaaataaa gggtcgaaga tgtgatttca
    34081 tatcttcgac ccttttttga attatattga tttaaagata caaaacatga gaggggggaa
    34141 ggaattgata aagaaccatt aaagatttat gatgtagtgg ttctttatca ttaaacacag
    34201 ctaatgtgta tttaaaaata ggaatacatg agtaaaactc atgtataaga aatactaatt
    34261 tctaaagaaa aagtatttct ttatgttggg gccccgtcaa ctactgccaa atacaacact
    34321 atagagtcta gacattgatt tatgtccgac tcccaagaat agttttactt ttttacaatc
    34381 actaatagat tgctaaaatc aaaatttcct tcaccactat ctacagtcga catttcattt
    34441 tttgaaatta tctacatttt ttcataccaa gatattttat agttatgata tttatgtaaa
    34501 aagaattata tagtaagtta gcttaaactt tactaaaaac gggtattaaa ctttgtatca
    34561 ttatttaaat ttttcatgta caatgtaata cagtaatctt atgaggtgat aaaatggatt
    34621 atatcggaca atatgcagtt atccatttag tgttacatgt tgtatgtatt tgtattgcct
    34681 attgggcttt acaatcaatt agattagatc aattttttaa aaaaggatac gccactcaat
    34741 tacaagtgtg tatgatattt gttgctattt tattaggcac tgcagtaagc aattttattg
    34801 tagatttgtt acaatactcg acgcaggtaa aatatttaat aaaataagtc taactctatg
    34861 atttgtaatc aaaactagat ataattaaat aatgacttaa aataatttta aaatagggaa
    34921 atgtaaagta ataggagttc taagtggagg atttacgatg gataaaatag taatcaaagg
    34981 tggaaataaa ttaacgggtg aagttaaagt agaaggtgct aaaaatgcag tattaccaat
    35041 attgacagca tctttattag cttctgataa accgagcaaa ttagttaatg ttccagcttt
    35101 aagtgatgta gaaacaataa ataatgtatt aacaacttta aatgctgacg ttacatacaa
    35161 aaaggacgaa aatgctgttg tcgttgatgc aacaaagact ctaaatgaag aggcaccata
    35221 tgaatatgtt agtaaaatgc gtgcaagtat tttagttatg ggacctcttt tagcaagact
    35281 aggacatgct attgttgcat tgcctggtgg ttgtgcaatt ggaagtagac cgattgagca
    35341 acacattaaa ggttttgaag ctttaggcgc agaaattcat cttgaaaatg gtaatattta
    35401 tgctaatgct aaagatggat taaaaggtac atcaattcat ttagattttc caagtgtagg
    35461 agcaacacaa aatattatta tggcagcatc attagctaag ggtaagactt taattgaaaa
    35521 tgcagctaaa gaacctgaaa ttgtcgattt agcaaactac attaatgaaa tgggtggtag
    35581 aattactggt gctggtacag acacaattac aatcaatggt gtagaatcat tacatggtgt
    35641 agaacatgct atcattccag atagaattga agcaggcaca ttactaatcg ctggtgctat
    35701 aacgcgtggt gatatttttg tacgtgatgc aatcaaagaa catatggcga gtttagtcta
    35761 taaactagaa gaaatgggcg ttgaattgga ctatcaagaa gatggtattc gtgtacgtgc
    35821 tgaaggggaa ttacaacctg tagacatcaa aactctacca catcctggat tcccgactga
    35881 tatgcaatca caaatgatgg cattgttatt aacggcaaat ggtcataaag tcgtaaccga
    35941 aactgttttt gaaaaccgtt ttatgcatgt tgcagagttc aaacgtatga atgctaatat
    36001 caatgtagaa ggtcgtagtg ctaaacttga aggtaaaagt caattgcaag gtgcacaagt
    36061 taaagcgact gatttaagag cagcagccgc cttaatttta gctggattag ttgctgatgg
    36121 taaaacaagc gttactgaat taacgcacct agatagaggc tatgttgact tacacggtaa
    36181 attgaagcaa ttaggtgcag acattgaacg tattaacgat taattcagta aattaatata
    36241 atggaggatt tcaaccatgg aaacaatttt tgattataac caaattaaac aaattatacc
    36301 tcacagacag ccatttttat taattgataa agtagttgaa tatgaagaag gtcaacgttg
    36361 tgtggctatt aaacaagtat caggaaacga accattcttt caagggcatt ttcctgagta
    36421 tgcggtaatg ccaggcgtat taattactga agcgttagct caaacaggtg cggtagctat
    36481 tttaaatagt gaagaaaata aaggtaaaat cgctttattt gctggtattg ataaatgtcg
    36541 ttttaaacgt caagtagtac ctggtgatac tttaacgttg gaagtagaaa tcactaaaat
    36601 taaaggacca ataggtaaag gtaatgctaa agctactgtc gatggtcaac ttgcttgtag
    36661 ttgtgaactt acatttgcaa ttcaagatgt aaaataaaac aaaaaaaaca ttcaaagatt
    36721 taatgtgttg gcataatctt tgaatgtttt ttattttact cttctaattt ttcatccttt
    36781 aactttggtt tagactgcat cattcgatta aatgattttt ttaattcttc accagataat
    36841 ccatcatcaa taagttgttc taataaactt tcagcatact gttgacgtaa agtgtaaata
    36901 tgacattcaa atacgataga cattgttgtt ttaaaatttt taatactttt cacagtagat
    36961 tcaaataaag ctggatcatt ggctggacgc gtgatgacga cacgaatatc caataactgt
    37021 tcattgtctt gatattcctt aaatgtttca taatgctcat tagaaatgac aatttcaagc
    37081 aaccaacccg tgccactttc ctctttatta attattacgc catcttctaa ttcatattct
    37141 ataattctgc cgtgttcatt cacaatttga aaccttactg ctttaaaagt tttcatattc
    37201 ccacatccca ttagtttaat atttatgatt tttgaattat ttagaagatt taaggtcatt
    37261 tcatgactaa tttctttaaa tataacaaat aacgagatgc tatagctatt aattatataa
    37321 aatttgccat tttgaccatt tttaagctag taattaagat ataggcatag gaggtgaatc
    37381 acacactaca tgctaaataa aatcgtaatt gtcgggagac tgacgaaaga cgcacaaata
    37441 tttgaaaagg aggatagaaa aattgcaacg ttttgtgttg caacgcaccg aaattataaa
    37501 gatgaaaatg gagaaatcgt ctgtgattac ttattctgta aagcatttgg caagttagct
    37561 tctaatatag aaaaatatac taatcaaggt acattggttg gtataactgg tcaaatgaga
    37621 tcaagaaagt atgataaaga cggacaaaca cactttgtca ctgaattata tgttgaaaca
    37681 ataaaattta tgtcccctaa atcccaaaat aatgaaattc tctcagatag tatttagata
    37741 ttgactctca aaatatagat aatcatgact tattagaaat ttaaatccaa ttatcctaaa
    37801 catccttaat atacatttaa tccatcctaa attttttata gatctaatta acttgaaata
    37861 tactcactta aaaacgactt acatgactag tactgttgtg taggtcgtct ttttgtgtag
    37921 tttttctaat agaaaataga catagattgc aaaacgatta cataaaaaat tgcaaataaa
    37981 aaaacatttt tactgtcatg acaattctcg atagaatcat atctgaaatt atgggggatt
    38041 tcgttgtaat cactgtgtaa aaaacaaggc tattttttta ttttgttgta acctgcacgg
    38101 attttataga tgttacagta gtctctgtaa aacttttaga gaaacaaatt acttgtagga
    38161 ggattttaag attatgaaga aaacattact cgcatcatca ttagcagtag gtttaggaat
    38221 cgtagcagga aatgcaggtc acgaagccca tgcaagtgaa gcggacttaa ataaagcatc
    38281 tttagcgcaa atggcgcaat caaatgatca aacattaaat caaaaaccaa ttgaagctgg
    38341 ggcttataat tatacatttg actatgaagg gtttacttat cactttgaat cagatggtac
    38401 acactttgct tggaattacc atgcaacagg tactaatgga gcagacatga gtgcacaagc
    38461 acctgcaact aataatgttg caccatcagc tgttcaagct aatcaagtac aatcacaaga
    38521 agttgaagca ccacaaaatg ctcaaactca acaaccacaa gcatcaacat caaacaattc
    38581 acaagttact gcaacaccaa ctgaatcaaa atcatcagaa ggttcatcag taaatgtgaa
    38641 tgctcatcta aaacaaattg ctcaacgtga atcaggtggc aatattcatg ctgtaaatcc
    38701 aacatcaggt gcagctggta agtatcaatt cttacaatca acttgggatt cagtagcacc
    38761 tgctaaatat aaaggtgtat caccagcaaa tgctcctgaa agtgttcaag atgccgcagc
    38821 agtaaaatta tataacactg gtggcgctgg acattgggtt actgcataag ccatttatgc
    38881 atagctaatt aatagttata taagtaactt ttagattgga atatatcggg gaattctcga
    38941 tatgttccgg tttttttatt tcgcaaatat atgtcattat agaattatta tttaaagata
    39001 ataatacaac tgagcaacaa taattgaaaa tgttgaaacc gtttgctaag attaatttgc
    39061 aattgaatac taaggaacta ctaggggagc ctaatgatat ggctgagatg aattgttcag
    39121 acccttatga cctgatttgg ttagtaccaa cgtaggaaag tagttattga agtgatgggg
    39181 ctagtatagc aacattgcca tcaaactact ttcatgttgg aagtagtttt tttatttagg
    39241 gaggattttt tacaatggaa ttttcacaaa aattgtacca agctgcaaag ccaatcatta
    39301 atgatattta tgaggatgac tttattcaga agatgttatc cggagatata ggagctgacg
    39361 cattacgcca ttacttaaaa gctgatgctg cgtatttaaa agaatttaca aacttatatg
    39421 ctttgttaat tccaaaaatg aatagcatga atgacgtaaa atttttagta gagcaaatag
    39481 aatttatggt tgaaggtgaa gtacttgcgc atgatatttt agcgcaaatt gttggtgaat
    39541 catacgaaga aatcattaaa acaaaagttt ggcctccaag tggcgaccat tacataaaac
    39601 atatgtattt ccaagcgcat agtcgtgaaa atgctattta tacaattgct gcaatggcac
    39661 cttgtccata tatttatgca gaattagcta agcgctctca aagtgatcat aagcttaata
    39721 gagaaaaaga tacggcaaaa tggtttgatt tttatagtac tgaaatggat gacataatta
    39781 acgtatttga ggcattaatg aataaattag ccgaatcaat gtctgacaag gaattggaac
    39841 aagtaaagca agtattttta gaaagttgca tacatgagcg tcgatttttc aatatggcta
    39901 tgacactaga acaatgggaa tttggaggaa aagtaaatga ttaaacctaa aatagcatta
    39961 accattgcag gtactgatcc aacaggtggt gccggcgtaa tggctgattt aaaatcattt
    40021 cattcatgtg gtgtatatgg tatgggcgtc gttacaagta ttgttgctca aaatacattg
    40081 ggcgtacaac atattcataa tttaaatcat caatgggtag atgaacaact tgatagtgtc
    40141 ttcaatgata ccttacctca tgctattaaa acggggatga ttgctacagc agatactatg
    40201 gaaacgattc gtcattattt aatgcaacat gaatctattc catatgtaat tgatcctgtt
    40261 atgttggcga aaagcggtga ttcattaatg gataatgaca caaagcaaaa cttgcagcat
    40321 acgttattgc cattagctga cgtagtaaca ccgaatttac cagaagctga agaaataacg
    40381 ggactaacca ttgatagtga agaaaaaatt atgcaggctg gccgcatctt tattaatgag
    40441 attggtagta aaggtgtcat cattaaaggc ggtcattcaa atgatactga tatagcaaaa
    40501 gattatttat ttactaacga aggtgttcaa acatttgaaa atgaacgatt taaaacaaaa
    40561 catacgcatg gaacagggtg tacattttca gcagttataa cggcagaact tgcaaaaggt
    40621 agaccattat ttgaggctgt acacaaggct aaaaagttta tttcaatgag tatacaatat
    40681 acgcctgaaa tcggccgtgg tagaggtcca gtgaatcatt ttgcatattt aaagaaagag
    40741 ggattagacg atgaattatc taaataaaat acgtattgaa aacccattaa caatttgtta
    40801 tacaaacgat gtagttaaaa attttacagc gaatggttta ttaagtattg gtgctagccc
    40861 tgcaatgagt gaagctcccg aagaagctga agaattttac aaagttgcac aagcgctatt
    40921 aatcaatatc ggtactttaa cagcagaaaa tgaacaagat attattgcga ttgctcaaac
    40981 ggcaaatgag gcaggcttac ctattgtatt tgaccctgta gctgttggtg cttctacata
    41041 tcgaaagcaa ttttgtaaat tattattgaa atcagcgaaa gtatcagtaa ttaaaggcaa
    41101 tgcatctgaa atattagcgt tgattgatga tacagcaact atgaaaggta cagatagtga
    41161 tgctaatctt gatgcggttg caatagcgaa aaaggcttac gcaacatata aaactgcaat
    41221 agtaatcaca ggtaaagagg acgttattgt tcaagataat aaagccttcg tattagctaa
    41281 tggatctcca ttattagcac gagtaactgg agctggttgt ttattaggag gcgttattgc
    41341 tggattttta tttagagaaa cagaaccaga catagaagcg ttaattgaag cggtaagcgt
    41401 atttaatatt gctgctgagg tagctgctga aaatgaaaat tgtggtggtc ctggtacgtt
    41461 ttcaccattg ttgcttgata cgttatatca tttaaatgaa acaacctatc aacaacggat
    41521 tcgtattcaa gaggtggaat aatatgttta accaatcgta tctaaatgtg tattttattt
    41581 gtgggacctc cgatgttccg agtcatcgaa ctattcatga agtattagaa gcagcattaa
    41641 aggcagggat tacacttttc caatttcgag aaaagggtga atcagcttta aaaggaaacg
    41701 ataaacttgt attagctaaa gaattacagc atctctgtca tcaatatgat gtgcctttta
    41761 tcgttaatga tgatgtatcc ttggcaaaag aaattaacgc ggatggtata catgtcggtc
    41821 aagatgatgc gaaagtcaaa gagatagcac aatatttcac tgataaaatt attggactta
    41881 gtattagtga tttagatgag tatgcaaaat ctgatttaac tcatgttgat tatataggtg
    41941 ttggaccaat ctatcctacg ccgtcgaagc atgacgcgca tataccagta ggtccagaaa
    42001 tgattgcaac gtttaaagaa atgaatccgc aactaccgat tgtagctatt ggcggtatca
    42061 atactaataa tgtggcgccg attgtagaag caggtgccaa tggaatttca gtcatttcag
    42121 ccatttctaa aagtgaaaat attgaaaaga ctgttaatcg attcaaagat ttttttaata
    42181 attagttaaa tttttccact tattgtttca tacgtactca ttaatgtgat agaataaatc
    42241 ctatgtgaat ataatgatag aggtggaaaa atgaagaaaa aagcgttact accattattt
    42301 ttaggtatta tggtcttttt ggctggttgt gactattcta aacctgaaaa acgtagtggg
    42361 tttttctaca atacattcgt agatccaatg aaaaatgtat tggattggtt gggaaataac
    42421 ttattaaacg acaattatgg tttagctatt attatccttg tattggtaat tcgtattatt
    42481 ttattaccat tcatgttgtc aaactataaa aatagtcata tgatgcgtca aaaaatgaaa
    42541 gttgcaaagc cagaagttga aaaaattcaa gaaaaagtga aacgtgcgcg tacacaagaa
    42601 gaaaaaatgg ctgcaaacca agaattaatg caagtatata aaaagtatga catgaacccg
    42661 attaagagta tgttgggttg tttaccaatg ctaatccaat taccaatcat catgggatta
    42721 tactttgtac ttaaagacca acttgtagat ggtttgttta aatatccaca cttcttatgg
    42781 ttcgatttag gacgtcctga tatttggatt acaattattg ccggtgtttt atactttatc
    42841 caagcatatg tatcaagtaa aacgatgcca gacgaacaac gtcaaatggg ttacatgatg
    42901 atggtcattt caccaattat gattatctgg atttcattaa gctcagcatc agcacttggt
    42961 ttgtactggt cagtcagtgc ggcgttcctt gtagttcaaa cacactttgc gaacatttat
    43021 tatgaaaaag tcgctaaaaa agaagtacaa cctttcattg aagcgtatga aagagagcac
    43081 aacggcggca gcaataaaaa aggcaaaaac acacaagttg tgtctaaaaa gaaaaagaaa
    43141 taaccttagc aagtccaaat aatgtgcacc ctaaaagttg aagataattt taactttata
    43201 gggcgcacat tatttttcgc ttcaatagcg aaacttagtg tcacattata caatatgtga
    43261 taaatagaaa cagcagtaag atgatttcaa atgtgaaagt catcctactg ctgtttttat
    43321 ttgaaataat attaacgagt tttcagtatg taaaaagtgg ttaataacag agttgtcatt
    43381 cttcatcgca atgtcatctt gacacaatga ttcatcagtg tagcagtata tgtcgcaaat
    43441 ttccacagca attcacttat acatgtaaca gtgtgaaata ataacgtcac aacggcataa
    43501 tacacattgc aacgtcccaa caacatatca tagaatcgta acggcatgac atcaacacca
    43561 aacctggcta taacaccata acagcgcaac gccacatcat agcactacat caagtcgcat
    43621 cgtagcacca gcacatcaaa gtaacatggc atcaacatca aaccggtcta taacaccata
    43681 acaccgcaat gccacatcat agcactacat caagtcgcat cgtggtacca gcacatcaaa
    43741 gtaacatggc atcaacgcca aacctgtcta taacaccata acaccgcaat gccacatcat
    43801 agcactacat caagacgcat cgtggtacca gcacatcaaa gtagcatgac atgtcaccaa
    43861 gctcccacaa caacacatca aagtaacatg gcatgacacc aagcttccac aacaacacgc
    43921 cattacatcg catcatcata ctacaacttc aatgtctagt cgtgacaatt ccattccgta
    43981 aaaaactgtt tcaagtacat catcataaag tcgtgacgtt ctttagcaat catcttcgcc
    44041 gtatctgtat gcattaaaga ttctaactta agtaattttt caaagaaatg tttaattgca
    44101 gatggtggca actgttcaac taaatcatca ttaatcttat ctagtgacat atgttctgtc
    44161 cacataggtt caccaaagtg tcctgcaaat tgaaatgttc gtgcaacacc tatagcgcct
    44221 atagcatcaa gacgatctgc atccctgaca atttgacctt ctaaagataa agtgacatga
    44281 tcatttttgc cattgcgata gctcatatta ttaataataa ataaaatgtg ctcttgatct
    44341 tcggttgata gtgataaaga agataaaaat gacttcaatt caacatattg tttgttagca
    44401 tctacaactt tttcgtcaac ggtatcatga agcaaacatg cgagttcaat gactaaagta
    44461 tcattaacac cttcattttc agcgattgat ttagctaaag cagtgacacg atatacatgc
    44521 gcaatatcat ggcctgtata atcattttga tgaatagatt ccatatattt tttggcagca
    44581 ctaatgacgt ctgattgttg cattaaagtt acactcctca tatttctatt ttataagata
    44641 ggtgacaata attgtgaaat accttcttta aatttaatcc aaagacttcg cttagcataa
    44701 cgtgcttttg ttaattcaga agatactgct aaatcatcta taaaagcttg ttttaatttt
    44761 ttggcaattt gttggtcata aataaaagcg ttgacttcga aattcaatgt gaaactgcga
    44821 tggtccatat tagctgttcc cacacttgca atttcatcat ctataacaag tgtttttgag
    44881 tgtaaaaagc cattgtcgta gtgaaatact ttaacaccgg catctaataa ggatgctgca
    44941 ttttttaaag tagcccaaaa aacaaacgga tggtcaggtt tattaggaat catgatattg
    45001 acatcaacac cacctaatgc cgcaatttta atagaatcta aaaaggcttg atcaggtatg
    45061 aaatagggag attgaatata aatcgatttt ttagcagatg aaatcatttt caaatagccg
    45121 tatttaatct gttcccattc ttcgtcagga ccactagaag ctatttgaac gccaattgtt
    45181 ccaccagaat ttacatctgg gaaataacga tcatcatagg agatgtggtc acgtgtggct
    45241 tgtgaattcc aatctagaat aaatcgtaat tgcaatgcat tcactgcatc cccgacaatt
    45301 cgtaaatgcg tatctcgcca atagccgaat ttttttgatt tacctaagta ctcatcacca
    45361 acattaaaac caccaacata tccaatttgc ccatctatta caacaatttt tcgatggttt
    45421 cgattgttca tacgcaagtt aattaaaggt aattttgatg ggaaaaatgc ttcagcatgt
    45481 ccacctttat tgcgaaacgg gcgtaagcct tttttacgca gtccacgaga acccatgtca
    45541 tcataaagaa ttttaacttc tacaccttgt tccgcttttt taccaagttc atttaaaatg
    45601 gtacgaccta attcatcatt ttgaataata tagtactgaa aatgaatata atcagtagca
    45661 ttacggatgt cttgtattag gtcatcaaat ttttcttggc cgtctgtgta tatttttaaa
    45721 tcgttgtctg ttgttaaaaa tgctgcgtta ttatataaca acatttgaat catttcttta
    45781 aattttacaa tttgataatt ggaatttgaa aagttttcat tttttaaagc agctaattgc
    45841 tcatcaacga ttaactctaa tcctttttta tcttccttat caattttgaa aatttggtca
    45901 cgttgaattt gtcgtcctaa tagtaagtat aaaatgaagc cgaataaagg caagaaaact
    45961 aagactagta accaagccca gatagaattg gcagaacgtc tttccatgaa aataatggta
    46021 aaggcgaata ataagtttaa aataaatgca ccaataaata ttgaatttaa aataatatta
    46081 gaatgcttga gtgcaatgga taataactct atcatttgaa acctcccatc gaaaatctta
    46141 aacgagtagt atatcttatt aattgtatca tgaagcaatt taaatatcat gacaatttag
    46201 tttgaaaaac acactttgtg ttgacgttgt gttttatggg atatagtata gtaaggaatg
    46261 taaatgaagg agtgaatgct atgactgaac aagataatgc acatcattct gaacaaataa
    46321 aaacgaatct taaatcacgt ttaaatcgaa ttgaaggaca agtgagagcg attaatcgca
    46381 tgattgaaga agatgtctat tgtgatgatg tccttacgca aataagagcg acacgttcgg
    46441 cgttaaacag tgttgcgata aagttattag aacaacatat gaaaagttgt attatgaata
    46501 aagttaatca aggtgctcag gaagaggcaa tggaagagtt attagtgact tttcaaaaat
    46561 tgattaaaga ctaaaggagt ttaagatgat acatcaaaat acgatttaca cagcgggaat
    46621 tgaaacagaa gaacaagtaa gtcaattgac agaacgcatt tcaaatatga taggtgttca
    46681 tcaagtgaat attaatataa tagatggtca agtaactgta tcgtatgaga caccagcaaa
    46741 tttgaatagt attgaaaaag aaatctatga tgaaggatac aaaattgtat tttagggtat
    46801 aatgtagagt gcgctataga ttttaatttt gaaaataaat taaaaatttt gtaaatgatg
    46861 tagtaaaggt atgtcgaata gggtaagata atattgaaag cgatccaaac gaggtgataa
    46921 taatgaaacg tcctgaaaag attcaaaatg tagtcaaact attgtcatca ttaggtgtga
    46981 atattaaaaa aactaaatct cgtttagaca ttattaatac tttgccagca tctaataaag
    47041 taagtcacga attaaaataa ttgactgtga ttgtgaatca cacaccgtta aagctatcgt
    47101 cggactattc gatgatagct tttttgactg gatttactaa aatgaaagca agtactatat
    47161 aacaaaatcc ctctttagag tgtacatttt acaatgtgta cttcaaagag ggattgttat
    47221 tatcgagtaa tatctatata tgacatttta aatcgacgac gtaaatagac tatataacgt
    47281 catcttgaaa agttatgata tttgaaactc aaaatagttt aaattaatta ctttttggat
    47341 ggtataaatc gacatatcgt tttggttcat gataataaat tgataagact atacctattc
    47401 cagtcatcat actccatagc gcactaccac cataactaat aaatggtaat ggaataccag
    47461 tgattggtaa caactgaatt gtcataccaa tattttgtaa aatatggaac acaagtaaag
    47521 tgacgaaacc aacgataaag attttgttaa attgatcttc aattttcgca gctaatctta
    47581 ttagatggaa gattaaaaat aaaaatatta agatcaatat gacagaaccg ataaagccaa
    47641 gttcctctcc aatcactgaa aagataaagt cagtatgatt ttcaggtata taaacttcac
    47701 cgtgattgta tcctttacct agtaactgtc cagaaccgat agctttaagt gattcagtta
    47761 aatgatagcc atcaccacta ctatatgtat aggggtcaag ccatgaattg attcgtccca
    47821 tttgatacag ttggacacct aataaatttt caattaatgc gggtgcatat agaataccta
    47881 aaatgactgt cattgcacca acaatacctg taataaagat aggtgctaag atacgccatg
    47941 ttataccact tactaacatc acacctgcaa taatagcagc taatactaat gtagttccta
    48001 ggtcattttg cagtaatatt aaaatacttg gtactaacga gacaccaata attttgaaaa
    48061 ataataacaa atcactttgg aatgatttat tgaatgtgaa ttgattatgt ctagaaacga
    48121 cacgcgctaa tgctaaaatt aaaataattt tcatgaattc agatggctga atactgatag
    48181 ggccaaacgt gtaccaactt ttggcaccat tgataatagg tgtaataggt gactcaggaa
    48241 taacgagcaa gcctattaat aatagacaga ttaagaaata caataaatat gtataatgtt
    48301 taatcttttt aggtgaaata aacatgatga tacctgcaaa aattgcacct aaaatgtaat
    48361 aaaaaatttg tctgataccg aaattagcac tgtattgacc accgcccatt gccgagttaa
    48421 taagcagaac actgaaaatt gctaaaacag ctatagtggc tactaatacc cagtctactt
    48481 tgcgaagcca atgcttatcc ggctgttgac gagatgaata attcattgca aactcctttt
    48541 atactcacta atgtttatat caattttaca tgacttttta aaaattagct agaatatcac
    48601 agtgatatca gctatagatt tcaatttgaa ttaggaataa aatagaaggg aatattgttc
    48661 tgattataaa tgaatcaaca tagatacaga cacataagtc ctcgttttta aaatgcaaaa
    48721 tagcattaaa atgtgatact attaagattc aaagatgcga ataaatcaat taacaatagg
    48781 actaaatcaa tattaattta tattaaggta gcaaaccctg atatatcatt ggaggaaaac
    48841 gaaatgacaa aagaaaatat ttgtatcgtt tttggaggga aaagtgcaga acacgaagta
    48901 tcgattctga cagcacaaaa tgtattaaat gcaatagata aagacaaata tcatgttgat
    48961 atcatttata ttaccaatga tggtgattgg agaaagcaaa ataatattac agctgaaatt
    49021 aaatctactg atgagcttca tttagaaaat ggagaggcgc ttgagatttc acagctattg
    49081 aaagaaagta gttcaggaca accatacgat gcagtattcc cattattaca tggtcctaat
    49141 ggtgaagatg gcacgattca agggcttttt gaagttttgg atgtaccata tgtaggaaat
    49201 ggtgtattgt cagctgcaag ttctatggac aaacttgtaa tgaaacaatt atttgaacat
    49261 cgagggttac cacagttacc ttatattagt ttcttacgtt ctgaatatga aaaatatgaa
    49321 cataacattt taaaattagt aaatgataaa ttaaattacc cagtctttgt taaacctgct
    49381 aacttagggt caagtgtagg tatcagtaaa tgtaataatg aagcggaact taaagaaggt
    49441 attaaagaag cattccaatt tgaccgtaag cttgttatag aacaaggcgt taacgcacgt
    49501 gaaattgaag tagcagtttt aggaaatgac tatcctgaag cgacatggcc aggtgaagtc
    49561 gtaaaagatg tcgcgtttta cgattacaaa tcaaaatata aagatggtaa ggttcaatta
    49621 caaattccag ctgacttaga cgaagatgtt caattaacgc ttagaaatat ggcattagag
    49681 gcattcaaag cgacagattg ttctggttta gtccgtgctg atttctttgt aacagaagac
    49741 aaccaaatat atattaatga aacaaatgca atgcctggat ttacggcttt cagtatgtat
    49801 ccaaagttat gggaaaatat gggcttatct tatccagaat tgattacaaa acttatcgag
    49861 cttgctaaag aacgtcacca ggataaacag aaaaataaat acaaaattga ctaactgagg
    49921 ttgttattat gattaatgtt acattaaagc aaattcaatc atggattcct tgtgaaattg
    49981 aagatcaatt tttaaatcaa gagataaatg gagtcacaat tgattcacga gcaatttcta
    50041 aaaatatgtt atttatacca tttaaaggtg aaaatgttga cggtcatcgc tttgtctcta
    50101 aagcattaca agatggtgct ggggctgctt tttatcaaag agggacacct atagatgaaa
    50161 atgtaagcgg gcctattata tgggttgaag acacattaac ggcattacaa caattggcac
    50221 aagcttactt gagacatgta aaccctaaag taattgccgt cacagggtct aatggtaaaa
    50281 caacgactaa agatatgatt gaaagtgtat tgcataccga atttaaagtt aagaaaacgc
    50341 aaggtaatta caataatgaa attggtttac ctttaactat tttggaatta gataatgata
    50401 ctgaaatatc aatattggag atggggatgt caggtttcca tgaaattgaa tttctgtcaa
    50461 acctcgctca accagatatt gcagttataa ctaatattgg tgagtcacat atgcaagatt
    50521 taggttcgcg cgaggggatt gctaaagcta aatctgaaat tacaataggt ctaaaagata
    50581 atggtacgtt tatatatgat ggcgatgaac cattattgaa accacatgtt aaagaagttg
    50641 aaaatgcaaa atgtattagt attggtgttg ctactgataa tgcattagtt tgttctgttg
    50701 atgatagaga tactacaggt atttcattta cgattaataa taaagaacat tacgatctgc
    50761 caatattagg aaagcataat atgaaaaatg cgacgattgc cattgcggtt ggtcatgaat
    50821 taggtttgat atataacaca atctatcaaa atttaaaaaa tgtcagctta actggtatgc
    50881 gtatggaaca acatacatta gaaaatgata ttactgtgat aaatgatgcc tataatgcaa
    50941 gtcctacaag tatgagagca gctattgata cactgagtac tttgacaggg cgtcgcattc
    51001 taattttagg agatgtttta gaattaggtg aaaatagcaa agaaatgcat atcggtgtag
    51061 gtaattattt agaagaaaag catatagatg tgttgtatac gtttggtaat gaagcgaagt
    51121 atatttatga ttcgggccag caacatgtcg aaaaagcaca acacttcaat tctaaagacg
    51181 atatgataga agttttaata aacgatttaa aagcgcatga ccgtgtatta gttaaaggat
    51241 cacgtggtat gaaattagaa gaagtggtaa atgctttaat ttcatagaga ttagtcgagg
    51301 gaccttttac ttataaaaat gatttgaatt aatactaaaa gattacaaag aagaggtggt
    51361 tttgtgtgta aatacaaaat tgcctttttc tttttatgtt aaatctataa atttgaaact
    51421 aaatcaaggt taattctatg tacacacttt atataggaag tagtttgaat gtttatataa
    51481 tgttttacaa aaagatgtag tattataatg tctaatttca catgtgtttc agtaaaa
//
LOCUS       DAKTGS010000005        51454 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.005, whole genome shotgun sequence.
ACCESSION   DAKTGS010000005 DAKTGS010000000
VERSION     DAKTGS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 51454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.005"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..295
                     /locus_tag="O0286_000239"
     CDS             <1..295
                     /locus_tag="O0286_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0168643.1"
                     /translation="QERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENK
                     EDDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
     gene            562..981
                     /locus_tag="O0286_000240"
     CDS             562..981
                     /locus_tag="O0286_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0168644.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            999..1418
                     /locus_tag="O0286_000241"
     CDS             999..1418
                     /locus_tag="O0286_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0168645.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            1870..2292
                     /locus_tag="O0286_000242"
     CDS             1870..2292
                     /locus_tag="O0286_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCY0168646.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(2404..3942)
                     /locus_tag="O0286_000243"
     CDS             complement(2404..3942)
                     /locus_tag="O0286_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCY0168647.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            4175..4993
                     /locus_tag="O0286_000244"
                     /pseudo
     CDS             4175..4993
                     /locus_tag="O0286_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            5256..5621
                     /locus_tag="O0286_000245"
     CDS             5256..5621
                     /locus_tag="O0286_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168648.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            6488..7309
                     /gene="cntK"
                     /locus_tag="O0286_000246"
     CDS             6488..7309
                     /gene="cntK"
                     /locus_tag="O0286_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCY0168649.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            7320..8138
                     /gene="cntL"
                     /locus_tag="O0286_000247"
     CDS             7320..8138
                     /gene="cntL"
                     /locus_tag="O0286_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCY0168650.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            8131..9432
                     /gene="cntM"
                     /locus_tag="O0286_000248"
     CDS             8131..9432
                     /gene="cntM"
                     /locus_tag="O0286_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCY0168651.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            9575..11173
                     /gene="cntA"
                     /locus_tag="O0286_000249"
     CDS             9575..11173
                     /gene="cntA"
                     /locus_tag="O0286_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCY0168652.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            11186..12121
                     /locus_tag="O0286_000250"
     CDS             11186..12121
                     /locus_tag="O0286_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0168653.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            12118..12987
                     /locus_tag="O0286_000251"
     CDS             12118..12987
                     /locus_tag="O0286_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0168654.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            12984..13799
                     /locus_tag="O0286_000252"
     CDS             12984..13799
                     /locus_tag="O0286_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0168655.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            13792..14541
                     /locus_tag="O0286_000253"
     CDS             13792..14541
                     /locus_tag="O0286_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0168656.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            14553..15746
                     /locus_tag="O0286_000254"
     CDS             14553..15746
                     /locus_tag="O0286_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0168657.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(15970..16719)
                     /locus_tag="O0286_000255"
     CDS             complement(15970..16719)
                     /locus_tag="O0286_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168658.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            16914..18491
                     /locus_tag="O0286_000256"
     CDS             16914..18491
                     /locus_tag="O0286_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCY0168659.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            complement(18659..19114)
                     /locus_tag="O0286_000257"
     CDS             complement(18659..19114)
                     /locus_tag="O0286_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCY0168660.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            19473..19730
                     /locus_tag="O0286_000258"
     CDS             19473..19730
                     /locus_tag="O0286_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0168661.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIRETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            19730..19996
                     /locus_tag="O0286_000259"
     CDS             19730..19996
                     /locus_tag="O0286_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0168662.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            20351..21427
                     /locus_tag="O0286_000260"
     CDS             20351..21427
                     /locus_tag="O0286_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0168663.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            21675..22337
                     /locus_tag="O0286_000261"
     CDS             21675..22337
                     /locus_tag="O0286_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0168664.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            22330..23106
                     /gene="fetB"
                     /locus_tag="O0286_000262"
     CDS             22330..23106
                     /gene="fetB"
                     /locus_tag="O0286_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCY0168665.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            23502..24689
                     /locus_tag="O0286_000263"
     CDS             23502..24689
                     /locus_tag="O0286_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0168666.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(24751..26103)
                     /locus_tag="O0286_000264"
     CDS             complement(24751..26103)
                     /locus_tag="O0286_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCY0168667.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(26711..28348)
                     /locus_tag="O0286_000265"
     CDS             complement(26711..28348)
                     /locus_tag="O0286_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0168668.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            28528..29127
                     /locus_tag="O0286_000266"
     CDS             28528..29127
                     /locus_tag="O0286_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCY0168669.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            29131..29271
                     /locus_tag="O0286_000267"
     CDS             29131..29271
                     /locus_tag="O0286_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168670.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            29351..29470
                     /locus_tag="O0286_000268"
     CDS             29351..29470
                     /locus_tag="O0286_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168671.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            29797..31023
                     /locus_tag="O0286_000269"
     CDS             29797..31023
                     /locus_tag="O0286_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0168672.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            31020..31655
                     /locus_tag="O0286_000270"
     CDS             31020..31655
                     /locus_tag="O0286_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0168673.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            31672..32613
                     /locus_tag="O0286_000271"
     CDS             31672..32613
                     /locus_tag="O0286_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0168674.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            32613..33308
                     /locus_tag="O0286_000272"
     CDS             32613..33308
                     /locus_tag="O0286_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0168675.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            33602..35002
                     /locus_tag="O0286_000273"
     CDS             33602..35002
                     /locus_tag="O0286_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0168676.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            35228..36163
                     /locus_tag="O0286_000274"
     CDS             35228..36163
                     /locus_tag="O0286_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCY0168677.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            36485..37336
                     /locus_tag="O0286_000275"
     CDS             36485..37336
                     /locus_tag="O0286_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0168678.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(37428..38924)
                     /locus_tag="O0286_000276"
     CDS             complement(37428..38924)
                     /locus_tag="O0286_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCY0168679.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            39156..40985
                     /locus_tag="O0286_000277"
     CDS             39156..40985
                     /locus_tag="O0286_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0168680.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(41125..43203)
                     /locus_tag="O0286_000278"
     CDS             complement(41125..43203)
                     /locus_tag="O0286_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0168681.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(43341..44750)
                     /locus_tag="O0286_000279"
     CDS             complement(43341..44750)
                     /locus_tag="O0286_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0168682.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            45036..45841
                     /locus_tag="O0286_000280"
                     /pseudo
     CDS             45036..45841
                     /locus_tag="O0286_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(45904..46560)
                     /locus_tag="O0286_000281"
     CDS             complement(45904..46560)
                     /locus_tag="O0286_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCY0168683.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            46673..47146
                     /locus_tag="O0286_000282"
     CDS             46673..47146
                     /locus_tag="O0286_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0168684.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(47269..48480)
                     /locus_tag="O0286_000283"
     CDS             complement(47269..48480)
                     /locus_tag="O0286_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0168685.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(48662..49321)
                     /locus_tag="O0286_000284"
     CDS             complement(48662..49321)
                     /locus_tag="O0286_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168686.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(49381..50523)
                     /locus_tag="O0286_000285"
     CDS             complement(49381..50523)
                     /locus_tag="O0286_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0168687.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            50791..51177
                     /gene="gtxA"
                     /locus_tag="O0286_000286"
     CDS             50791..51177
                     /gene="gtxA"
                     /locus_tag="O0286_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCY0168688.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            51311..51418
                     /locus_tag="O0286_000287"
     CDS             51311..51418
                     /locus_tag="O0286_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0168689.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT        17447 a         7626 c         9750 g        16631 t
ORIGIN      
        1 acaggagcgt attacactct atcttaagca taatactaaa gaacccaata ccatcaaaac
       61 tgtacatttc acaagtttaa aaagaggacc tatgggcgat gcagtaattg aaggttacat
      121 aaatgaaaat aaagaagatg attttgttgc atatgggtct ccagaacata attatcaatt
      181 tggtggaagt ttaatcaaaa gtaaaaattt aagcacgtta ttaaaaccag tacatcaaac
      241 caaatcacct gatgagataa aaaaggaatt agagtctaaa aagaacgacc gctaaaacta
      301 tgcggccgtt ttaattttgt tttcaagtta atcatactta catataaaaa tgaaaagttg
      361 aatttttagt acaatttcca ctgtatttat ctgttaacca ctttataccc gcaatatttc
      421 gttgatctta tataaacact tttggctgct tttccttgac ctagattaaa attatagatt
      481 ttctttgtaa gagtgtgttt tgtagtatca taaatatgta aactgtataa aattccacca
      541 cattttagga gctggtgata gatgtcgaaa aagaaaattt taatattaac tagtattatg
      601 ttaatcatat taattagtgt tgcaggtatc tatttaaaag tgaaatatga cgaaaaagaa
      661 aaacaaaaag caatatacta taaagaacaa caagcgcgca ttacacttta tctcaagcat
      721 aacactaaag aaccgaatac aatcaaatct gtacatttca caaactcgga aacaagtcct
      781 atgggaagtg ctgtgattga aggttacata aatgaaaaca aaaaagataa ttttactgct
      841 tatgctacac cagaacataa ttatcaattt ggtggtgcta tgatagaaag tgaaaaatta
      901 agcgagttac taaagccagc caatcagtta aaatcaccag atgatataaa aaaagaacta
      961 aataaaaaga agagtcacta aagttaggag ttactttaat gtccaaaaaa catgttttta
     1021 taattattgg tgtcatattg tgtatatgta cagtttctac ggtcatgcat tttaaaatga
     1081 aatatgatga aaaagaaaaa caaaaagcga tttactacaa agaacaacaa gaacgtatta
     1141 cactctatct taagcataat actaaagaaa cgaacacgat taaatctgta catttcacaa
     1201 acttggaaac aagtcctatg ggaagtgctg tgattgaagg atacatcaat gaaaataaag
     1261 aagatgattt tactgcttat gcatcgcctg aacataatta tcaatttggt ggcgctatga
     1321 taaaaagtga aggagtagat aaattattaa aaccagcaca tgaaagaaaa tcaccagaaa
     1381 aaatcaaaga agaattagat aaaaaagaag gccactaggg tcttctttat ttttgattta
     1441 atcttccaat aatctatgtc attgctatcg aaggtgtttc gcaattaata taaatcactt
     1501 catcatcacc aatacttccc cagttttgta cagtacatta acacaaacga accacgttaa
     1561 tttaaatgga ataatagttt ggccattata agaacaatat atatcgaata acaatgtcga
     1621 tgacgtgatt tattgacatt atcaaatttt ctcaaatgtg tgtgattttt tataaaaaag
     1681 ggtagagcaa ggtataatat cctttcaatc tgttttcata ttttatatat tttttaaata
     1741 taagtgctaa atgttttaac taaagcatag attgacaaga tgttatacag aatttcaaat
     1801 tctatccaat attgttcgaa gtgtagtatc actggattgg tattaaacaa tgtaaaggag
     1861 agattgcaaa tgccgtataa ttacaagaaa caaaatggag agttaatgtc tgtaatgagc
     1921 caaggtgaaa agtttattca tcaatcaccc gttaatgatg aacttagtgc attgattaag
     1981 ttattaattt ctaaaattaa cggttgtcat tattgtgttg atatccataa aaaagaatta
     2041 aaggaattgg gtgtaacaca aatgaaaatt gatgaagtct tgagttttag acatttagat
     2101 ttatttactg atcaagaaaa agtgacgctt gaatttgcag aaatgttaaa ttcaatcaaa
     2161 gactttaaga agtttgaaat tattgaccgg ctaaaatcat tttatgatga agaacaaatt
     2221 attgatcttg tctttgttgt aaaccaaatt aacggttgga acagattaaa tattattagt
     2281 gatagactat aattgttcat ataaatgcag agtttcatct cgaacgctat atcataacaa
     2341 atcatgccac tatacaggtc aaatcttgta tagtggcatt ttaatttatc cctttgaata
     2401 ctgttattta acgaatatcg gtccacctgg tccaacaggg atacctaata ggaaccaaat
     2461 gatgacaaat actgtccata caatacttag cgcgattgaa tacggcatta aactagaaag
     2521 taaggctccg agtttcatgc gtttatcgta tttttgtgca taagttaata ataaaggtaa
     2581 gtacggcatc atcggtgtaa ttggattggt aattgaatcg cctacacggt aaatgacttg
     2641 tgtgaatgcg ggatgaaagc cgataaggat taacattggt acgaatatcg gtcctaaaat
     2701 accccattta gccgatgcgc ttccgattaa catgttgacc attgcactca gtacaataat
     2761 acctagtatc aatacaatac cgttttgatg ttctaataat ttggcacctt taacagcagc
     2821 gataattcct aaattactcc actttaaata cgcaagtagc tgtgctgcaa aaaacacaat
     2881 aacgataaat gttcccattg atcctacagc atcgccgaac attttaccta agtcttttgt
     2941 atttttaatt tctttgctta aaatcccata aactaatcca ggtactaaaa atacgacaag
     3001 aataattaat ccgacaccgt taattaatgg cgcatcgtct agtaagctgc ctgttttagc
     3061 atttcttaaa aagctatgtt caggaatggc tgtaataatt aataaaataa ttgtgactat
     3121 gaaactgata tttgcccatt ttaaagcatg tgcttctttg tcagtaatat gtgaagatgt
     3181 ttcttcatgg tcatcatgca ttaaactgtc atcatatttt cctaatctag gaataatgag
     3241 cttagttgta actagcaata tcgtaggaag taatacaacg acactcgctg cgataaagta
     3301 ccagttcata gcaacgtttg ttttaataga atctgaaacg atacgtgttg ccggttctgt
     3361 aaatgaatag accaaagcat cttgcatacc aacaactata tttgctgcaa atcctccaac
     3421 agcggaagca tatgccatcg ttagtccagc gatagggtga tagccaattt taataaaaag
     3481 cattgctgca agcggcggca agataattgt cgcagcatcg ccggctgtac tacctaaaat
     3541 accaattaat ataatagtcg gtaaaattaa gaagcgtggt gcgcgattca caacagaaat
     3601 cattaactta tcgaagtatc ctgttttctc tgcaacacca ataccaatca tcactgctag
     3661 tactaagcct aatgctggga actctgagaa atttttaatc gtatcattca ttatcatcgt
     3721 aaatccatca tggctaatta tatttttaat ataaatggtt tgatgcgtac ctggatgctt
     3781 aacagataca ttaaataatg agataaccca tgtcataatg gctaagccca cacacattaa
     3841 aaagaataag acgctaggat ctggcaattt atttccgatt ttttcaacac tattcaagaa
     3901 acgattgacg atagacccct tttgttgatg ttttgatgtc atcaattatt cccccctttg
     3961 ttaaatattt aaagtgtaac aaaaaatact ctcaaaagta acaattttca gaaaataaaa
     4021 aaactaatat tgttaaatat tttgagttat tcaatagaaa gtgtatagca gagtagttaa
     4081 gactgcctga agacttatct attaggttta tgaagcatcg aacagtggaa aataaggact
     4141 gtaagtttaa gatatgttgt ataggagtga ctgaatgaaa cgtttggaaa ataaagtagc
     4201 agtcgtaaca ggagcaagta caggtatcgg tcaagcttct gcaatcgctt tagctcaata
     4261 aggtgcgtat gtattggcgg tagacatagc tgaagcggta tcggagactg tcgataaaat
     4321 taaaagtaat ggtgacaatg cgaaggcgta taatgtggat aatgcaagcg aacaacaagt
     4381 ggtagacttt gtgtctgaca taaaggaaca gtttggaaga atcgatgtgt tgtttaataa
     4441 tgccggtgtg gataatgcgg ctgatagaat tcatgagtat ccaatagatg tgtatgacaa
     4501 gattatgaat gtagatatgc gtgggacatt tttaatgacg aaaatgatgt tacctttaat
     4561 gatgaatcaa ggtggctcta ttgttaatac gtcttcattt tccggacaag cagcagactt
     4621 gtatcgctct ggatataatg ctgcgaaagg tgcagtgatt aattttacaa aatcaatcgc
     4681 aattgagtat ggccgtgata gcattcgagc caatgcgatt gcaccaggta caattgaaac
     4741 cccgttagta gataaactga caggtacgag tgaggatgat gcaggtaaaa catttagaga
     4801 aaatcaaaaa tggatgactc cgctgggacg tttaggtaaa ccagaagaag ttgctaaatt
     4861 agtagtcttc ttagcatctg acgacagttc attcataact ggagagacga ttcgaattga
     4921 tggtggtgtg atggcttaca catggcctgg cgagatgtta agtgatgatt cgtggaagcg
     4981 gacattggaa taattattta aaatcgatat ccagtggaaa agaatttggc atgaagtagg
     5041 caatgtatgc atattgtcgc aatgaaagac atttaaagcg gagattaatt aatctttgaa
     5101 aatcacatat tgttcatttg aagtgtattg cttaaagctt gttttaacgt attgtaggtc
     5161 gtgcatctaa tttatcagaa tctcgagttc aaacttttgg agtatcttta aaataagctg
     5221 tattttatct attctaataa attaaggaga attttatgtt aaaagaaaaa gaaagtttta
     5281 gattgctata tcaagctata agagagattg cagataaaat tggagataat cagttagaaa
     5341 ctaattccgt tagtttatta ttattggact ttgattttga acatgaagta tttgatgaat
     5401 tgtatcttgt gattttaaaa tatttaaata cagtaagtat agagaacata agtcatagtg
     5461 agcttttgaa tttaatagaa aatacgattc cagaagatcg agagattaac acgtttgtaa
     5521 aaaataaaat aataattggc tttgctaata attacttccc tgaattacaa gtattagcaa
     5581 acgaaataaa atctgatatg gctagttcat taaaacaatg atatttttat ttaaattttt
     5641 aaagctttgt acgaaattgt acaaagcttt tttggtgcgt attgtatggg caacaacttg
     5701 acgatgaaaa tccgttacag gattggtaat aggaaatgtt agcgaaagac aagggtatcc
     5761 attgtagatt aacaaaagga cgtttccaca agtgtgggtt attctcacta aagcaatacg
     5821 cagagacaac ttacgtaaaa ttttgaactg actagaacgg aacttctact caattattga
     5881 taaaaatttt caaaaagact tgaatgtgct gagaatacga agtttatgga aggattatca
     5941 aaatataaat gtgcattcat ttacaacctt tattgacaat gattctcaac taatatagta
     6001 tataatcaaa tcgtaatagt tacgatttgt tttctgcaac ttttttgaag ttttagttga
     6061 ggtgaaaaca gtaaaagcat ctaagtgaat gtagttaacg gacaactgca ttcgcttgta
     6121 gagccacaag aagcaacttt aaataaggtt tacggttgca ttttgataca acaaccgatt
     6181 actaagtcat gctttccact ttgcgggtta gcatgactta cctaatagat agagctatta
     6241 ggttcagctt ctaaaaaatt acagttttag aggaatacag ttgcttgctt cgcaacaact
     6301 gcataagagc catggttttc gcttttgcga attagcatga cttacctact agatagagct
     6361 attaggttca tcttctaaaa aattacaggt ttagaggaat acagttgttt gcttcgcaac
     6421 aactgcataa gagcctctag taattaaaat tacagaggct ctaaaaatac atctaaagga
     6481 gtgtcgtatg aatcggcagg ttatagaatt ttctaagtat aatccttcgg ggaatatgac
     6541 gatacttgtt cattcaaaac atgatgctag tgaatatgca tctatcgcca atcagttgat
     6601 ggccgcaaca catgtatgct gtgaacaggt aggctttata gaatcaacac aaaatgatga
     6661 tggtaatgat tttcacttag ttatgagcgg taatgaattt tgcggtaatg cgacgatgtc
     6721 atatatacat catttgcagg aaagtcattt gcttaaagac caacagttta aggtgaaggt
     6781 gtctggctgt tcggatttag tgcaatgcgc aattcatgat tgccaatact atgaagttca
     6841 aatgccacaa gcccatcgtg ttgtgccaac aacaattaat atgggtaatc attcatggaa
     6901 agcaatagaa attatttatg aaacatatgt acattatgtg attccagtta aacaagtaac
     6961 aacagaaatt caacatttgg ttgaagcgtt tgtgcgtgag caacaatgga gtcacaaata
     7021 taaaacagta ggtatgatgc tttttgatga acaacgtcaa tttttacagc cattaatcta
     7081 tataccagaa attcaaagtt taatttggga aaatagctgt ggttctggta cagcatcaat
     7141 tggggttttt aataattatc aacgtaatga cgcatgcaaa gattttacag tacatcagcc
     7201 agggggcagt attttagtga catcaaagcg atgtcatcaa ttgggatatc aaacttcaat
     7261 taaaggacag gttacaactg tagctacagg aaaagcatat atagaataag gagcctacaa
     7321 tgaataactt taataatgaa atcaaattga tattacaaca atatttagaa aagtttgaag
     7381 cgcattacga gcgtgtatta caagacgatc aatatatcga agcattagaa acattgatgg
     7441 atgactatag tgaatttatt ttaaatccta tttatgaaca acaatttaat gcttggcgtg
     7501 acgttgaaga aaaagcacaa ttaataaaat cactgcaata tattacagcg cagtgtgtta
     7561 aacaagtgga agtcattaga gcgagacgtc tattagacgg acaggcgtct accacaggtt
     7621 actttgacaa tatagaacat tgtattgatg aagagtttgg acaatgtagt atagctagca
     7681 atgacaaatt attgttagtt ggttcaggtg catatccaat gacgttaatt caagtagcaa
     7741 aagaaacagg tgcttcagtt atcggtattg atattgatcc acaagccgtt gacctagggc
     7801 gcagaatcgt taacgtctta gcaccaaatg aagatataac aattacggat caaaaggtat
     7861 ctgaacttaa agatatcaaa gatgtgacgc atatcatatt cagctcgaca attcctttaa
     7921 agtacagcat tttagaagaa ttatatgatt taacaaatga aaatgtcgta gttgcaatgc
     7981 gctttggtga tggcatcaaa gcaatattta attatccgtc acaagaaaca gcggaagata
     8041 agtggcaatg tgtgaataaa catatgagac cacagcaaat ttttgatata gcactttata
     8101 aaaaagcagc tataaaggta ggtattacgg atgtctaaat tattaatgat aggcactggt
     8161 ccggtcgcaa tccaattagc gaatatttgc tatttaaaat cagattatga gattgatatg
     8221 gttggacgtg cctcaacatc agaaaaatca aaacgcttat atcaagcgta taaaaaagcg
     8281 aaacaatttg aagtcaaaat acaaaacgag gcgcatcaac atctggaagg taagtttgaa
     8341 attaatcgtt tgtataaaga tgttaaaaac gttaagggtg aatacgaaac ggttgtcatg
     8401 gcatgcacag cagatgctta ttatgacaca ctacagcaat tgtcgttaga aactttgcaa
     8461 agtgtcaaac atgtcatttt aatatcaccg acatttggtt cgcaaatgat tgtcgaacaa
     8521 tttatgtcta aatttaataa agatatcgaa gtgatttcat tctcaactta tcttggcgat
     8581 acacgtattg ttgataaaga agcgcctaat catgtgttga caacaggtgt aaaaaagaaa
     8641 ttgtacatgg gatcgacaca ttcaaactca acaatgtgtc aacgaatctc tgctttagct
     8701 gagcaattga agattcaatt agaagtcgtt gaatcgccac tgcatgctga aacgcgaaac
     8761 agttcgcttt atgtgcaccc accactattt atgaatgact tttcattgaa agccattttc
     8821 gaaggaacag atgtaccggt ttatgtgtat aagttatttc ctgaaggacc gataacgatg
     8881 acactaatcc gtgaaatgcg tttaatgtgg aaggaaatga tggttatttt acaagcattt
     8941 agagtgccgt cagtcaacct gcttcaattt atggtgaagg aaaattatcc agtacgtcct
     9001 gaaactttgg atgaaggtga tattgagcat ttcgaaatct tgccagatat cttacaagaa
     9061 tatctgcttt atgtaagata taccgcaatc ctcattgatc cattttcaca gccagacgaa
     9121 aacggacatt actttgattt ttcagctgta ccatttaagc aagtctataa aaatgaacag
     9181 gatgttgttc aaattccaag aatgccaagt gaagattatt acagaacggc gatgattcag
     9241 catattggga aaatgctagg tatcaaaacg ccaatgattg atcagttcct aactcgctat
     9301 gaagcaagtt gccaggcgta caaggatatg catcaagatc aacacttatc ttctcaattt
     9361 aatacaaatc tatttgaagg agataaagca ctcgtcacaa aatttttgga aatcaataga
     9421 acgctttcat aataagggtt tgaagtttta taatagaaaa aaattattga attatgtttg
     9481 acatttacat aaaaataagc aaataattga gaaaaataat cattacgatt tgattaagta
     9541 atgcaactta tcaatttaga aagaggaaaa gcaaatgaga aaactaacta aaatgagtgc
     9601 aatgttactt gcatcagggc taattttaac tggttgtggc ggtaataaag gtttagagga
     9661 gaaaaaagaa aacaagcaat taacgtatac gacggttaaa gatatcggtg atatgaatcc
     9721 gcatgtttac ggtggatcaa tgtctgctga aagtatgata tacgagccgc ttgtacgtaa
     9781 cacgaaagat ggtattaagc ctttactagc taaaaagtgg gatgtgtctg aagatgggaa
     9841 gacatacacg ttccatttga gagatgacgt taaattccat gatggtacgc catttgatgc
     9901 tgacgcagtt aagaaaaata ttgacgcagt tcaagaaaac aaaaaattgc attcttggtt
     9961 aaagatttcg acattaattg acaatgttaa agttaaagat aagtacacgg ttgaattgaa
    10021 tttgaaagaa gcatatcaac ctgcattggc tgaattagcg atgcctcgtc catatgtatt
    10081 tgtgtctcca aaagacttta aaaacggtac aacaaaagat ggcgttaaaa agttcgatgg
    10141 tactggtcca tttaaattag gtgaacacaa aaaagatgag tctgcagact ttaacaaaaa
    10201 tgatcaatac tggggcgaaa agtctaaact taacaaagta caagcaaaag taatgcctgc
    10261 tggtgaaaca gcattcctat caatgaaaaa aggtgaaacg aactttgcct tcacagatga
    10321 tagaggtaca gatagcttag acaaagactc tttaaaacaa ttgaaagata caggtgacta
    10381 tcaagttaag cgtagtcaac ctatgaatac gaaaatgtta gttgtcaatt ctggtaaaaa
    10441 agataacgct gtgagtgaca aaacagtcag acaagcgatt ggtcatatgg taaacagaga
    10501 taaaattgcc aaagaaattt tagatggtca agaaaaacca gcaactcaat tatttgcgaa
    10561 aaatgtaaca gacattaatt tcgatatgcc aacacgtaag tatgacctta aaaaagcaga
    10621 atcattatta gatgaagctg gttggaagaa aggtaaagac agcgatgttc gtcaaaaaga
    10681 tggtaaaaac cttgaaatgg caatgtacta tgacaaaggt tcttcaagtc aaaaagaaca
    10741 agcagaatac ttacaagcag aatttaagaa aatgggtatt aagttaaaca tcaatggcga
    10801 aacatcagat aaaattgctg aacgtcgtac ttctggtgat tatgacttaa tgttcaacca
    10861 aacttgggga ttattgtacg atccacaaag tactattgca gcatttaaag cgaaaaatgg
    10921 ttatgaaagt gcaacatcag gcattgagaa caaagataaa atatacaaca gcattgatga
    10981 cgcatttaaa atccaaaacg gtaaagagcg ttcagacgct tataaaaaca ttttgaaaca
    11041 aattgatgat gaaggtatct ttatccctat ttcacacggt agtatgacag ttgttgcacc
    11101 aaaagattta gaaaaagtat cattcacaca atcacagtat gaattaccat tcaatgaaat
    11161 gcagtataaa taaaggagca attagatgtt caaatttatc ttaaaacgta ttgcgctcat
    11221 gtttccattg atgattgtag taagttttat gacatttcta ttgacgtata ttacaaatga
    11281 aaatccagct gtgacaattt tacatgcaca agggacgcca aatgtaacac cagagttgat
    11341 tgcagaaacg aatgagaagt acggtttcaa tgatccatta ttaattcaat ataaaaattg
    11401 gttacttgaa gcgatgcaat ttaattttgg tacaagctac attacaggtg acccagttgc
    11461 tgaacgtatt ggtccagcat ttatgaatac attgaaatta acaataattt caagtgttat
    11521 ggtgatgatt acatcaatta ttttaggtgt agttagtgca ttaaaaagag gaaagttcac
    11581 tgatcgtgcg atacgttcag tggctttctt tctaactgca ttaccatcat attggatagc
    11641 ttcaatactt attatttacg tttcagtgaa gttaaacata ttgccgactt ctggattaac
    11701 aggtccagaa agttacatat tgccagtgat cgttattacg attgcctatg ctggtattta
    11761 ctttagaaat gttagacgct cgatggtgga acaattaaat gaagattatg tactttattt
    11821 aagagcaagc ggtgtgaaat ctatcacatt aatgttgcat gtgttgcgta atgctttaca
    11881 agttgcggta tcaatctttt gtatgtctat accaatgata atgggtggac tagttgttat
    11941 cgagtatatc tttgcatggc ctggactagg tcaattaagt ttaaaagcaa tacttgaaca
    12001 cgattttcca gtcattcaag catatgtatt aattgtagcg gtattattta ttgtatttaa
    12061 tacattagca gatatcatta atgcgctatt aaatccaaga ttaagggagg gcgcacgatg
    12121 ataattttaa aacgattatt acaagataaa ggtgcagtaa ttgctttagg cattattgta
    12181 ttatatgtct ttttaggatt agcagcacca cttgtgacat tttatgatcc taaccatatc
    12241 gatacagcaa acaaatttgc tggcatgagt tttcaacatc tactaggtac tgaccattta
    12301 ggtagagata ttttaactag gttaatttat gcgattagac caagtttgtt atatgtcttt
    12361 gttgcgctat ttgtttctgt acttattgga tctattttag gattcttatc aggatatttc
    12421 caagggtttg ttgacgcctt aatcatgcgt gcgtgtgatg ttatgttggc attcccaagt
    12481 tatgttgtaa cgttagcatt aattgcattg tttggaatgg gtgccgaaaa tattatcatg
    12541 gcatttattt tgacgcgttg ggcatggttc tgtcgtgtta tacgtacaag tgttatgcag
    12601 tacactgctt ctgaccatgt aagatttgct aaaacaatcg gtatgaatga tatgaaaatt
    12661 attcacaaac atattatgcc attaacatta gcagatattg ctatcatctc tagtagctcg
    12721 atgtgttcaa tgatcttgca aatatctggc ttttcatttt taggattagg tgtcaaagcg
    12781 cctactgcag agtggggcat gatgcttaac gaagctagaa aagtgatgtt tacacatcct
    12841 gaaatgatgt ttgcgccagg tattgccata gtgattatag tgatggcatt taacttctta
    12901 tccgatgctt tacaaattgc tattgatccc cgcatctctt ctaaagataa acttcgttct
    12961 gtgaaaaaag gagtggtgca atcatgacat tgttaacagt taaacatttg acgattacag
    13021 atacctggac agatcaacca ctcgtgagtg atgtgaattt tacattaact aagggtgaaa
    13081 ctttaggcgt tattggagaa agtggtagtg gtaaatcaat cacttgtaaa tcgattattg
    13141 gtttgaatcc cgaacgactc ggggtgacag gtgaaattat ctttgatggt acatcaatgt
    13201 tgtcattatc tgaatcgcaa ttgaaaaagt accgtggtaa agacattgcg atggtcatgc
    13261 aacaaggtag tcgtgccttt gacccatcaa ctactgtcgg taaacaaatg tttgagacta
    13321 tgaaagtaca tacgtcaatg tctacacaag aaattgaaaa gacattgatt gaatatatgg
    13381 attatttaag tttgaaagat cctaaacgta tattaaaatc atacccttac atgttatcag
    13441 gaggaatgtt acagcgattg atgattgctt tagcgttagc tttgaaacca aagttaatca
    13501 ttgctgatga gccgacaacg gctttagata caattacaca atatgatgta ctggaagcat
    13561 ttatagatat taaaaaacac tttgactgtg cgatgatttt catttcacat gatttaacgg
    13621 ttattaacaa gattgcagac cgtgttgttg tgatgaaaaa tggtcagctt attgaacaag
    13681 ggacacgtga atcagtcttg catcatccag aacatgttta tacgaagtat ttattatcaa
    13741 cgaagaagaa gattaatgat cattttaaac atgtgatgag gggtgatgta catgattaaa
    13801 attaaagatg ttgaaaagtc atatcaaagc gcacatgttt ttaagcgtcg tcgaacacct
    13861 atcgtgaaag gtgtgtcatt tgagtgtcca atcggtgcga cgattgcgat tatcggagaa
    13921 agtggtagcg gtaaatcgac gttgagtcgt atgatattag gtattgagaa accggataaa
    13981 ggttgtgtaa ccttaaatga tcaaccgatg cataagaaga aagtgagacg tcatcaaatt
    14041 ggtgctgtat ttcaagatta tacgtcatca ttacatccat ttcagactgt tagagaaatc
    14101 ttatttgaag tgatgtgtca atgtgatgga caacctaaag aagttatgga agtccaagca
    14161 attacattgt tggaagaagt cggtctatct aaggcataca tggataaata tcctaatatg
    14221 ttatcaggtg gagaggcgca acgtgttgcg attgcgcgtg caatatgtat taaccctaaa
    14281 tatattttgt ttgatgaagc cattagttca ctcgacatgt caattcaaac acaaatatta
    14341 gatttattga ttcatttacg tgaaacgcgt cagttgagtt atatttttat cacacatgat
    14401 attcaagctg ccacgtattt atgtgatcaa ttaattattt ttaaaaacgg aaaaatagaa
    14461 gaacaaattc cgacaagcgc attgcataaa agtgacaatg cttatacaag agaattaata
    14521 gaaaaacaac tatcattcta aggagtgaga taatgaaagg tgcaatggct tggccctttt
    14581 tgagattata tatattaaca ttgatgttct ttagtgccaa tgcaatctta aacgtgttta
    14641 tacctttacg agggcatgat ttaggcgcaa cgaatacggt tatcggtatc gttatggggg
    14701 catacatgtt aacagcaatg gtatttcgac catgggcagg acaaattatt gctcgtgtcg
    14761 gtcccattaa agtattaaga attattttga ttatcaatgc catagcttta attatttatg
    14821 gttttactgg cttagaaggt tatttcgtag cacgtgttat gcaaggtgtg tgtacggcat
    14881 tcttttctat gtctttacag ctaggtatta ttgatgcatt accagaggaa catcgttctg
    14941 aaggtgtatc attgtactcg ctattttcaa cgattccaaa cttaatcgga ccattagttg
    15001 ccgtaggtat ttggaatgca aataatattt cactatttgc aattgtcatt atctttatcg
    15061 cattaacaac aacattcttt ggctatcgcg tgacctttgc tgaacaggaa cccgatacgt
    15121 cagataagat tgaaaaaatg ccgtttaacg ctgtaactgt ttttgcgcaa tttttcaaaa
    15181 ataaagagtt gttgaacagt ggtattatca tgattgttgc atcgattgta tttggtgcag
    15241 ttagtacatt tgtaccgtta tacacagtga gtttaggatt cgcgaatgcg ggaatctttt
    15301 tgacaataca ggccatcgca gttgttgcgg caagatttta cttaaggaaa tacattccgt
    15361 cagatggtat gtggcatcct aaatatatgg tatctgtact atcattatta gtaatcgcgt
    15421 catttgtagt ggcatttggt ccgcaagtag gtgcaattat tttctatggt agtgcgatat
    15481 taataggaat gacgcaagca atggtgtacc caacattaac atcatactta agcttcgtct
    15541 taccaaaagt aggtcgtaat atgttgttag gtttatttat tgcctgtgca gacttaggta
    15601 tatcgttagg tggcgcattg atgggaccta tttccgattt agtaggattt aaatggatgt
    15661 atctaatttg tggtatgtta gtcattgtaa taatgattat gagtttcttg aaaaagccaa
    15721 caccacgtcc agcgagtagt ctttaatgaa gtgaattaaa gcatattaag ttaatgaata
    15781 tttaaatttt aaaaggtata ttgagcatgg cgattcatgt gcttcatgct aggacatgaa
    15841 acattctata tggctcgttt ttagaacgac agtatatcta aataaagcac gcttaaaagt
    15901 gagttttgat actgactttt aagtgtgctt tttgatgtgt atggataatg gataatactc
    15961 tgaaaaattt tacatgaaaa tatgcaaaac gagtataact gctaattgat agaaatagct
    16021 caccataaaa ttacggtatg attttaaata taagtaagtc gcactacctg ctagtatcaa
    16081 tgctggaatg aattcccacc atgtattaat gtatggatag tagaacagag tttcaaggat
    16141 aatggacaat actattgtaa tctttaaagg tattaatctg cttaattctt gaattaaaat
    16201 atgacggaaa ataagttgac aaatcaaagt atttaatata atggttaacg aaaatatagt
    16261 tattaaactg atggaaccat accctttaat gagcgggtaa atgtcaaaga cagtaaagga
    16321 atctacattt agtgcgaaaa tattgaaatg atttaaaagt aaaaagagta cgacacttag
    16381 tgtaaatgat ataagaatat gccatttata tttagcacta gcaacgattt gcgaacgtat
    16441 cattggaata aacgcatctt catgcatcag acgaaaaata gctagtgaaa taataactgc
    16501 gagtaaatag ctaatgttca ttgaaatagg aaaagagaaa ccccacggag cttgttgagt
    16561 gaatacagct actaacccaa aagttaaaaa gacgataatg atcggcaaga tgttaaccaa
    16621 aaatatgtaa aggaaaataa atccaatatc acgtttgaaa aaacgcgatt gttcggtagc
    16681 gtattcttct tctatgtaat gtttatttgt atttgacata gtatacctct taaatagttg
    16741 tattatatag atactttagc acatattact ttgtattgta tgttttatac attaaaattt
    16801 aaaatgaaaa acatatcata aaattgtttt ataaaatgaa gcgcttccat tgtgttttgt
    16861 tttgtaaggt gtatcataaa tattgaattg aaattttggg gggaggtatt gtaatgacgt
    16921 ttcttacagt catgcaattt atagttaaca ttatcgttgt aggattcatg cttacggtta
    16981 ttgttatcgg gcttatttgg ttaattaaag ataaaagaca atcacaacat agtgtattaa
    17041 ggaattatcc tttactagca cgtattagat atatttcaga aaaaatggga ccggaattac
    17101 gtcagtattt attttctggg gataatgaag ggaaaccttt ttcacgtaat gattataaaa
    17161 atatcgtttt ggctggaaaa tataactctc gtatgaccag cttcggtact actaaagatt
    17221 atcaagacgg cttttacata cagaacacaa tgtttccgat gcaacgtaat gagatttcag
    17281 tagataatac aacattgtta tcaacattca tttataaaat cgcgaatgag cgtttattta
    17341 gtcgtgaaga atatcgtgtg ccgacaaaga ttgatccgta ttacttaagt gatgaccatg
    17401 caataaaatt aggtgaacat ttaaaacatc catttatttt aaaacgtatc gtaggacaat
    17461 ctggtatgag ttatggcgct ttaggaaaaa atgccattac agctttatct aaaggtctag
    17521 ctaaagcggg cacttggatg aatacaggtg aaggtggctt atcagaatat catttaaaag
    17581 gtaatgggga tatcattttc caaattggtc ccggtttatt tggtgttcgt gataaagaag
    17641 gtaattttag tgaaggttta tttaaagagg ttgcacagtt atctaacgta cgcgcatttg
    17701 agctgaagtt ggcacaaggt gctaagactc gtggtggtca tatggaagct gaaaaggtaa
    17761 acgaagaaat tgctaaaatc cgaaatgttg aaccttataa aacaatcaat tcacctaacc
    17821 gttacgaatt tattcataat gctgaagatt tgattcgttt cgtcgatcag ttgcagcaat
    17881 taggtcaaaa accagtagga ttcaaaattg tagtaagcaa agtttcagaa attgaaacac
    17941 ttgtacgtac gatggtggaa ctagataagt atccaagctt tattacgatt gatggtggtg
    18001 aaggtggtac tggtgcaaca ttccaagaat tacaagatgg tgttggctta ccgctattta
    18061 cagctctacc tattgtgtct ggcatgttag aaaaatatgg tattcgagat aaagtgaaat
    18121 tggcggcatc tggtaagtta gtgacaccag ataaaattgc gattgcacta ggtttaggtg
    18181 cagattttgt aaatatcgca cgtgggatga tgattagtgt cggttgtata atgagtcaac
    18241 aatgtcacat gaatacgtgt cctgtaggtg ttgcaacgac agatgcgaag aaagaaaaag
    18301 cattgattgt tggagaaaag caatatcgtg tcacaaacta tgtaacaagt ttgcatgaag
    18361 gcttattcaa tattgcagca gctgttggcg tatccagtcc tacagaaatt actgctgatc
    18421 atattgtata tcgaaaagtc gatggtgagt tacaaacgat acatgattat aaattaaaac
    18481 tcattagtta acttaattat ttcgggaaat tgaaagcagc ggattttagc gttactgcaa
    18541 ataattttat attagtagtg gatgctggtc acacaagaac ttcaaatatt aaagccctca
    18601 gaatatgaat taaggtttgt aaccttagtc ttatctgagg gcatttttaa gttataaact
    18661 atttgtcgtc cattttatct ttttctttta aacctctgtg ctttaattgc ttttcaagtt
    18721 tttcaaaact aatatcttta ttttctttag tcgaaacacc aagacgttta tttaattttt
    18781 tcatgtcaac ttctgtgtaa tctatgtcta agtgttcaat tgctttttta tctttatagt
    18841 ctactttgta ttttacgcct ttaaggtctt tgaaaatact ttcagatttg gcgaataact
    18901 ttttggcttc gtctttatcc atacctagat cgtcatattt aattgtgttg attgtagact
    18961 gttttaaaac tttatcatct ttatatgtga tagaagttag tacatgttta ccactaacat
    19021 caccttcata tgttttggtt tgttctttac cacaagctga taatgcaatg atacaaacta
    19081 atgctactac aattaatgaa cataattttt tcaaagtcag tcgccttctt tcgatatttg
    19141 tattataaag aaattataac atttactaaa aaatgatgtt attcaaaaat ttaaattttg
    19201 tcattttttt tgaagatatg agttttttta agcggattcc tcacaaaatt ttaaaaatat
    19261 ttaagcctga aaatgataaa gcggtaggga acgtagtgtg ttccactgct gatttctttt
    19321 tgagcattat aggcggtatg ttggaagaaa agaacaaaag tttgtgaacg atagcatata
    19381 ggtattaaga atacgatgaa ctcgttggta atattgacgt actcaagtgc gtacgctata
    19441 attagcgtaa cagaaaacaa aggagggttt atatgattat cactagccct acagaagcga
    19501 gaaaagattt ttatcaatta ctaaaaaatg ttaataataa tcacgaacca atttatatta
    19561 gtggcaataa tgccgaaaat aatgctgtga ttataggttt agaagattgg aaaagtatac
    19621 gagagacaat atatcttgaa tctactggta caatggacaa agtaagagaa agagaaaaag
    19681 ataatagtgg tacaacaaat atagatgata ttgattggga taatctttaa tgagcaatta
    19741 cacggttaag attaaaaatt cagcgaaatc agatttaaag aaaataaaac attcttattt
    19801 aaagaagtca tttttagaaa ttgttgagac tttaaaaaat gatccgtata aaataacaca
    19861 atcttttgaa aaattagagc ctaaatattt agagcgatat tcaagaagaa ttaaccatca
    19921 gcacagggtc gtctataccg tagatgatcg aaataaagaa gtattaatac tatcggcatg
    19981 gtcacattat gattaatgaa tattcaatat ctgaataact ttaatgataa gttaattaaa
    20041 gaaactagta tttaagtgta gggaaaatag cgacgttaat gcgttgttat ctctacactt
    20101 tttaatttta taatagcgca agactaaaca gattgaaatt agtaacaata aaagaataac
    20161 gtattataat aaggaatttt aaattgtgac tttttcggaa tattaaattt tagaaatatg
    20221 aggtttttaa gcggattcct cacaaaattt taaaaatatt taagcctgaa aatgataaag
    20281 cggtagggaa cgtttttctg aaagttagtg atacaatagt tttaagttga aatacaggag
    20341 gatgaataac atgaatcagt cagtcaaatt acttaaacat ttaacagatg taaacggcat
    20401 tgctggttat gaaatgcaag ttaaagaagc aatgcgtaac tatatagagc ctgtcagtga
    20461 tcaaattatt gaagataact tgggtggcat ttttggaaag aaaaatgctg agaatggtca
    20521 atactcaatt atgatttctg gtcatatgga tgaagttggt tttatggtaa caaagattga
    20581 taaacatggt tttatttcat ttacgccagt tggtggatgg tggaatcaag tcatgctatc
    20641 tcaaaaagta acgattacaa cagattcggg caaagaaatt agaggtatca tcggttctaa
    20701 accgccacat gtcttaacgc ctgaagaacg taaaaagcca atggaaatca aaaatatgtt
    20761 tatagatatt ggtgttagta gcaaggaaga agctgaagaa gctggcgttg aagtaggcaa
    20821 tatggttacg ccatatagtg aatttgaagt gcttgcaaat gataaatatt taactgcgaa
    20881 agcatttgat aatcgctatg gctgtgcatt agctattgag gtattaaaac gtttaaaaga
    20941 tgaaaatatt ggcattaact tatacagtgg tgccacagtg caagaagaag ttggtttgcg
    21001 tggtgcgaaa gtggcagcga atacgattaa accagacttg gcgatagctg tcgatgtagg
    21061 tattgcttat gataccccag gtatgtcagg tcaaacgagc gatagtaaac taggcggtgg
    21121 tccagttgtc attatgatgg atgctacaag tattgctcac caaggtttgc gaaagcatat
    21181 taaagatgta gctaaggaac ataacatcga agtacaatgg gatacgacac caggtggagg
    21241 tacagatgcg ggaagtattc atgtcgcaaa tgaaggtatt ccaacgatga caatcggtgt
    21301 tacgctgcga tacatgcatt ctaatgtttc agtgctcaat gtagatgatt atgaaaattc
    21361 tatccgtctt gttactgaaa ttgtccgttc attgaatgat gaaagttata aaaatatcat
    21421 gtggtaatca aatccataaa taataaagaa tccttttaat atggtaggtt gttaaacaat
    21481 tgtctaattt taattcttag tcattagaca gtatccatgt taataggatt ttttgttttt
    21541 aatttaaatg ctgaaaatca attatgccta aattttgata ttacaagaaa atgatttttt
    21601 cttaaatgta attgcactaa aaaccaaaaa aacgggaata atatacctga tatattacat
    21661 gaggagcggt gcaaatgttg ttagaaatta aagatttagt gtataaagcg agcgatagaa
    21721 tcatactaga tcatatcagt ctaaaagtag ataaaggcga gagtattgcc attataggtc
    21781 catcaggtag tggtaaaagt acatttcaaa agcaaatatg taatttgttt agtccaacta
    21841 gtggagaact ttattttaaa ggtaaaccct ataatgatta tgacccggaa gaattgcgtc
    21901 aacgaatcag ttatttgatg cagcaaagtg acttgtttgg tgaaacgatt gaagataaca
    21961 tgatattccc atcacttgca cgtaatgata aatttgatag aaaacgtgca aagcaattaa
    22021 ttaaagatgt cggtttggga cattatcaat taagttcgga agtggaaaat atgtcgggtg
    22081 gtgagcggca aagaattgct atagcgcgcc aactgatgta tacaccggat attcttttat
    22141 tagatgaatc gaccagtgca ttagacgtta ataataaaga aaagatagaa aatatcattt
    22201 ttaaattagc agatcaaggc gtggcaatta tgtggattac ccacagcgat gaccaaagta
    22261 tgcgacactt tcaaaagcgt ataacaattg ttgatggtca aatttctaat acagaggagt
    22321 tgaatcaaca tgagtaatac ggcacttgga ttgacggcat tgcttttagt cattccgatt
    22381 atcatttcat ataaagaagg tttacatatt attaaagatt taattgttgc gacattacga
    22441 gcagttgtgc aattaatcat tttgggattt ttgctgcatt atatttttaa aataaacgat
    22501 aaatggctgc ttattttatg tgtattggtc attattatta atgcatcatg gaatacaatt
    22561 agtcgagcat caccagtgat gcatcatgtg ttttggatat catttctagc tatcttcatt
    22621 ggaacggcat taccgcttgc aggtactatt gcgacagggg ccattcaatt taccgcaaat
    22681 gaagttatac ctatcggcgg catgcttgca aataatggct tgattgcaat taatttagct
    22741 taccagaatt tagatcgtgc attcgtacaa gatggtacta atattgaatc taaattatca
    22801 cttgcagcta cacctaaatt ggcttctaaa ggtgcaatac gtgaaagtat tcgtttagct
    22861 atagtgccaa ctattgattc ggttaaaaca tatgggcttg tgtcgattcc tggtatgatg
    22921 acaggcttaa ttattggtgg cgtaccacct ttacaagcga ttaaatttca attgttagtc
    22981 gtgtttattc atacaactgc gaccattatg tctgctttga ttgcgacata tttaagctat
    23041 ggtcaatttt tcaatgcaag acatcaatta gtagcacgaa atactgatgt taagagtgaa
    23101 tcatgataga ttttactgca tcagatttag gcattagttt taattggaaa tgaagtgacg
    23161 cgcacatata gtatcgctat tcattagcgc agcgaaaata ttcataaagg cacgcatact
    23221 ttgtagtcag ttatctgttc tgacatataa agcgtgcgtg cttttttgga gttattgttg
    23281 aaactgaagt aattatacat aattattaaa tgacatactt gtgttaattt ttcaaatact
    23341 gaaaaacaat ttcaataatt ttccaattaa gcacagaaaa ttaaagcaaa atattatata
    23401 atagaacggt tatatataaa attgtgttgc acacattttt taataaatcg ttattctaag
    23461 ggaaatgaat atcggaaatt ttgtttgaaa ggagttttaa attgtcaatc atgcgactat
    23521 ttacattcat tttaagtatt tttatcgtag gaatggttga aatgatggtt gcaggaatta
    23581 tgaacttgat gagtcaggac ttacatgtat cagaagctgt cgttggtcaa ttagtgacaa
    23641 tgtacgcttt aacatttgcg atatgtggac ctattctggt taaattaacg aaccgttttt
    23701 catcaaggcc tgtattatta tggacattac ttatatttat cattggtaat ggcattattg
    23761 ctgtagcgcc aaatttttca atattagtag ttggtagaat tatctcatct gcagcagcag
    23821 cactaattat cgtaaaagta ttagctatta cagcgatgtt atcagcacct aaaaatcgtg
    23881 gtaaaatgat tggacttgtc tatacagggt ttagtggtgc taatgttttt ggtgtaccaa
    23941 ttggaacggt tatcggcgat ttagtaggtt ggcgctatac atttctattc ttaattattg
    24001 tgagtattat tgttggcttc ttgatgatga tctatttacc gaaggatcag gaaatacaac
    24061 gaggccctgt gaatcatgag acaccatctc atgaaaatca tgttacttcg aaaatattaa
    24121 gacctgctga agtagcaaaa tatttaatta ttacgttttt agtattgatt gcaaactcag
    24181 tgacattcgt ctttataaat ccacttattt tatctaatgg tcatgatatg tcattcgttt
    24241 cattagcact tctagtaaat ggaatcgctg gcgttattgg aacatcatta ggtggtatat
    24301 tctccgataa aattacaagt aagcgttggt taatgatttc tgtttctatt tttatcgtca
    24361 tgatgttact tatgaattta atcttacctg gttcaggtct attgttagca ggactattta
    24421 tttggaatat catgcaatgg agtactaatc cagcagtgca aagcggtgtg attcaacatg
    24481 ttgaaggcga cacaagccaa gtaatgagtt ggaacatgtc tagtttaaac gctggtattg
    24541 gtgttggagg cattattgga ggcttggtca tgacacatgt ttctgttcaa gctatcacat
    24601 atacgagtgc catcattggc gcattaggat taatcgttgt tttcacattg aaaaataatc
    24661 attatgctaa aacatttaaa tcatcataat tctcatatga aaagcacgcc tgctatcaaa
    24721 ttcaggtgtg cttttttaga tgcgataacg ttattgatat gtgcgataat agcgacgttc
    24781 attatgatac atcggccaag gcattttacc gcttttagca aaattagcta aatcattttg
    24841 catttgtcga cttaaaaatt taaggtgagc agttgttgga tattgatgtg ctgctaatat
    24901 ttgtaagttt ccgaaccaaa ataccatatc tagaatatgg taagcactgc gatagtgtgc
    24961 gctagaagta tcatgccatg caaattcagc aagccatagt tgtgcgtttg aatcttgtat
    25021 attgagttgt tgtaaaaatt gcttcatcgg ctgtttgaaa taacaatgtg taatcgctaa
    25081 agattgttgc ttcgccgtct gaacatcttc gtattttaaa ggaatatcat ttaattccat
    25141 aatgtcgata aagcgttgcg gcgataattt tttctgttca ctagtaatat aaatatcgcc
    25201 ttcatctttt gtataacatg caaaaattgg tttcgttgtt ggataattat tttgtatata
    25261 atctgtttta ataggcgcat atattaaatc aagcccttta gaaggtcctc gagattgttt
    25321 tagcttcgcc atcagcataa gaatatcatt tgtcgataat gatgtaacat catctgtatc
    25381 taaataatcg agcatcattt tttggaaatg ttgtgcttta ttgcgtgcac tctcaagggt
    25441 gtctaatcgt agtgcgccac ttagtagaac gactttatgg aagtatggct caatgtcagg
    25501 tattttaagt aaagtcaaaa tgctcatact gcctgcagac tgacccatta aagtaatgtt
    25561 attagcgtcg ccaccgaagg attcaataaa ttgatgcacc cattttatga cattgatttg
    25621 atctgaaagg ccattattgg aatgaaaatc tttattaaaa tatgaccagt ctaaatatcc
    25681 taatgcgcct aaacgataat tgcatgtaat aacgataatg tcgttatttt gtactaaatg
    25741 tgccggttga tagagttcgg ctgtaccatg accattttca aaactaccac cataaaaata
    25801 aatgatgaca ggtttcttcg tctgatcatt atgttgtttc caaatattta gatataaaca
    25861 gtcttcatgt tcagtaaaag ttgtagattg tgaggagaaa aaatcttcta atttgttgtc
    25921 tggttgcggt gggatgggtt gtatttcagt tgcatcaatt ggctctgacc attgtgtttt
    25981 taacgtagaa tgtttaaagc gattgtcatg aactggtggt tctgcataag gaatgcctaa
    26041 gaagatatct aaaccatctt gtgtaatacc atgaatttga ccacctgtag tattaatctt
    26101 catatttatt aacctccttt atcatcccca atttaatcat accacggtct atagatgata
    26161 ggtgacaagt ataacaagac aattttgtga ctgaatcaga cgagcttgat atctgtttta
    26221 actcaaacca ttttcagcat tatatgatgt acttctacgg gagtgggaca gaaatgataa
    26281 agagccacaa atgatttatt atgtagtggt tcttatacat tagccacagc taatgtgtac
    26341 ttaaaaatat gaatacatga gtaaaactca tgcataagaa atactaattt ctatagaaaa
    26401 agtatttctt tatcgtcgtc ccaccccaac ttgcattgcc tgtagaattt cttttcgaaa
    26461 ttctctgtgt tggggcccct gactagaatc gaaaaaagct tgttacaagc gcattttcgt
    26521 tcagtcaact actgccaata taacttcgta gagcatagaa cattgattta tgtcccagcc
    26581 tgatcatcat cttgttatgt tagttattta taaccgattt tcaaaataaa aaaccaacca
    26641 agatgttgaa atcaatggtt ggttaatgtg tgtgtgttga aatcatatat ggcactgtat
    26701 tttaaaatat ttaatgagat gactttgatt cattatttag tgactcacgc atcttcgtat
    26761 tgattcgtgt tgcgcgacgg aaatagacgc tttcaaatga actcgttgta ccgatgtaat
    26821 agaagataag tgccacaata ataataacga taaagtcaaa tggatagtga atcatattta
    26881 agcctttaaa ttctttgctt cctataaatg acagtattga tagcacgatt aaatatacaa
    26941 taatccataa gctaccacca atttgtttct ttgtattacg ccaattcata cgatattcat
    27001 agaagaagta gattggtaat ccaagtataa tgattaaaat aacttcagcc gttgttggcc
    27061 acattgccca atatatagct aatgaagcta atacaaatga taatggtgcc attactttta
    27121 aaatttttgc tctaaatgga cgagtcattg ttggtcccat ttttcttaat gcaatcactg
    27181 tcgttgggcc agttaaatag gctactaaag ttgcagtaga aataactgct gctagcgtac
    27241 cccaatctct aaataatgta accataatca tactaatgat ggcattaaag atgattgcta
    27301 cacgtgggat atgatatttt tcattcatct tcccaagaaa tttagggata tgtccatttt
    27361 tctccattgc tcgtaaaact cgacctgtaa cggcgacaaa tgacacgcca gtaccgaatg
    27421 gtgatacaaa agcttcaatg tatagtaaaa ttgcgagcca attaattcct aataagatag
    27481 ctaaatcagc aaatggtgaa ttgaagttga tgccattcca tccactatgt tgtaacattg
    27541 attgaggcat agaagtgata aacgtacttt gtaaaatgat gtacaacact gcactgattg
    27601 acagtgagat agcgatgcct cttgcgatat ttttttcagg atttttaatt tctgaaccca
    27661 tattaataat tgtctggaat gaattgaatg aaaaaataat ccctgatgct gttgttgcag
    27721 caaaaatcgg tgcacttccg taaggcataa atgtgcttgc cgaatggccg taatttgaag
    27781 tgtcgaatcc tgatagcatc aacatgatga tggttaacat gggtacgcct aatttaaata
    27841 cagaaattaa actcgtaaat gatgttaaaa gttttacgga ccaatagttt aataatgaaa
    27901 aaataacaat gatgagatat acagctagca atccgtatgt gctaatagag ccattttcca
    27961 ttaaatatct cattggtttc gcccaatgcc acggccaaga actcatatat tgaacagctg
    28021 acacagcttc gataggtatt attgtcacca aagacaccca attcgcccaa gcagcaataa
    28081 agcctaataa tgagccatgt gtatactggg catagttact catgccacct gattgaggaa
    28141 acattgtgcc gatttcaatg tagttatagg caatggttcc aatgactagg aatccaagaa
    28201 cccatgatat gattgctgct ggtccagcta ttgatgatgc ttcccacgca ccaaatagcc
    28261 aaccagatcc tattaaagag cctaacccta ataagactaa ttgggataga ttgattttgt
    28321 cgttgtgcaa ttgcttgtct ttattcataa atactccttc gttattgcca aaaatacaaa
    28381 aaataatatg cgacaattat acgcttatta taataatagt caaatgcatt ttttgtaaga
    28441 acgcaaaagc atttgtaaaa ttagtttttt ataaaattag gatatataat acaatgtgta
    28501 ttgaaaaaca aaatggaagt gatgactatg agtgagaaac aatcaaatga acaagttgaa
    28561 gcatttttaa ataaagagag tcaatggcaa gaagaatata aatatttgag agcgttaatc
    28621 tttaatgaaa cagaattaga ggaagcgtat aaatggatgc atccttgtta cacgttgaat
    28681 aataaaaatg tagtacttat ccatggcttc aaaaattatg ttgcactatt atttcataaa
    28741 ggtgccattt tggaggataa atatcataca ctcattcaac agactgaaaa ggtgcaagca
    28801 gctcgtcagt tacgatttga aaatttaaca gagattcaag cacgtaccga agaaattaaa
    28861 tattatctag ccgaagcaat taaagctgaa aaagctggta aaaaagttga aatgaagaaa
    28921 acagaggaat atgttattcc taaagagtta gaagctaagt tcgaagaaat gccacagtta
    28981 gagtcttcat tttataaatt aacgccaggc agacaacatc aatatatata tcatattgga
    29041 caagctaaac gcagtgaaac aagacaaaag cgtgttgaaa agtatattaa ccaaatacta
    29101 gaaggtaaag ggatgcatga taagtaatta atgagtaaag cataccggtt atacaacaac
    29161 atacaagatg acacgaaaca accaatggct catgctgttg gttgtttttt taggtgtgtc
    29221 tgtcatgggc aacactttga cgttggaatt ccgttacagg cttgggagta gaaaatgtta
    29281 gcaaaaggca agggtgtcta caatgaatga tgaagatatt aaaatataag gatgactttg
    29341 tgagtggcgg atgggcggtt gtccgtctgt aacaatggat gcgtgtgcat tattacaaaa
    29401 attcgacttt tgtaataata tttcacattt tcgacacttt tttgctataa aacaaccaat
    29461 tgagcgataa taaattcgct tttaaaaaat atgagttatc tatttagttg ccaaagataa
    29521 aataataatg tttaataaca tcatatagag tatgttagtt ttaaatgtcg aatatacgaa
    29581 tgtgcaaaca aagtaatcgg tagaaattca acatacatag cgccgtttac tgttaagtat
    29641 tcacattaca gatgaaaaat ataaaattct acataatcaa gaccatgatg tgtacttgtt
    29701 taacttatga ctctatttgt ttaacaattg cgataatggt ctttttattt tatgcgtatc
    29761 attcgtcata ttttttatga ggaaggagaa atgattatgt taagtattaa gcatttaacg
    29821 aaaatttatt ctggtaataa aaaggcagta gatgacatct ctttagatat tcaatctggg
    29881 gaatttatcg catttattgg aaccagtgga agtggcaaaa cgactgcttt aagaatgata
    29941 aaccgtatga ttgaagcgac agaaggacaa attgaaattg atggtaaaga tgttcggagt
    30001 atgaatcctg tcgaattgcg tagaaatatt ggctatgtta ttcaacaaat tggcttaatg
    30061 cctcatatga cgattaaaga gaatattgtg ttggtaccca aattgttgaa atggactaaa
    30121 gaggaaaagg ataaacgtgc aaaggaatta attaaacttg tggatttacc ggagtcattt
    30181 ttagagcgtt atccagcaga aatatcaggt gggcaacaac aacgtatcgg tgttgtaaga
    30241 gcacttgcgg ccgaacaaga tattatttta atggatgaac cttttggtgc attggatcct
    30301 attacgagag atacgttaca agatttagtt aaaacgttac aacgaaaatt aggcaagacg
    30361 tttatctttg taacacatga tatggatgaa gcgattaaat tagcagacaa aatttgtatt
    30421 atgtcagaag gtaaggtggt gcaatttgat acgccagaca atattttaag acatcccgca
    30481 aatgattttg tacgtgattt tataggacaa aatagactga ttcaagaccg tcccaatgac
    30541 aagactgtag aaggtgtaat gattaaacca atcacgatac aagcagaagc aacactgaat
    30601 gacgccgttc atattatgag acaaaaacgt gttgatgcta tttttgtagt agatagtaat
    30661 aaccatttac taggtttctt agacattgaa gatataaatc agggtatacg tggacacaaa
    30721 agtttacgag acaccatgca acaacatatt tataccgttc aaattgattc taaattacaa
    30781 gattctgtac gtacgatttt aaaaagaaac gttaggaatg tacctgtcgt agatgatcaa
    30841 cagcgtttag taggactgat tacgcgtgcc aatgttgttg atattgtata tgacacgatt
    30901 tggggcgata gtgaggatac agtgcaaaca gaacatgtgg gggaagacac tgcgtcctca
    30961 aaagtgcatg agcaacacac tactaatgtc aaagtacgtg acataggaga tgataaatca
    31021 tgattgagtt cctacatgaa catggtggac agttgatgtc gaaaacactg gaacatttct
    31081 atatttctat agtggcatta ttacttgcca tcattgttgc agtacctata ggcattttat
    31141 tatcaaaaac aaagcgaact gccaatattg tattaactgt ggcaggtgtc ttacaaacta
    31201 ttccaacact agctgtactt gctattatga taccgatttt tggtgttggt aaaacgcctg
    31261 caattgtagc gctatttatt tatgtattat tacctatttt aaataacacg gtactcggtg
    31321 ttcaaaatat tgatagcaac attaaagaag ctggaaaaag tatgggaatg acacaatttc
    31381 aattgatgaa ggatgttgaa ttgccgttag cattgccgct tatcattggt ggcattcgtt
    31441 tgtcatctgt gtatgtaatt agttgggcta cacttgcaag ttatgtaggt gcgggtggat
    31501 taggtgattt cattttcaat ggtttaaatt tatatgatcc actgatgatt gtaactgcaa
    31561 cggtactcgt tactgcacta gcattaggtg ttgatgcctt attagcttta gttgaaaaat
    31621 gggtagttcc caaaggctta aaagtatctg gataattagg aggctaagat aatgaagaaa
    31681 attaaatata tacttgtcgt gtttgtctta tcgcttaccg tattatctgg atgtagtttg
    31741 cccggactag gtagtaagag cacgaaaaat gatgtcaaaa ttacagcatt atcaacaagc
    31801 gaatcgcaaa ttatttcaca tatgttacgg ttgttaatag agcatgatac acacggtaag
    31861 ataaagccaa cattagtaaa taatttaggg tcaagtacga ttcaacataa tgccttaatt
    31921 aatggggatg ctaatatatc aggtgttaga tataatggca cagatttaac gggagctttg
    31981 aaggaagcac caattaaaaa tcctaagaaa gcaatgatag caacacaaca aggatttaaa
    32041 aagaaatttg atcaaacgtt ttttgattcg tatggttttg cgaatacgta tgcattcatg
    32101 gtaacgaagg aaaccgctaa aaaatatcat ttagagacag tttcagattt agcaaagcat
    32161 agtaaagatt tacgtttagg tatggatagt tcatggatga atcgtaaagg cgatggctat
    32221 gaaggattta aaaaagagta tggttttgac tttggtacag tgagaccaat gcaaataggt
    32281 ctagtctacg acgcattaaa ctcagagaag ttagacgttg cattaggtta ttctacagat
    32341 ggtcgaattg cggcgtatga tttgaaagta cttaaagatg ataaacaatt tttcccacct
    32401 tatgctgcga gtgctgttgc aacaaatgaa ttattacggc aacacccaga acttaaaacg
    32461 acgattaata agttgacagg aaagatttcg acttcagaga tgcaacgctt gaattatgaa
    32521 gcggatggta aaggtaaaga acctgctgtc gtcgcagaag aatttttgaa aaaacaccac
    32581 tattttgata aacagaaagg tggtcataag taatggaagg taatttatta cagcaattat
    32641 tcaattatta tgttacgaac tttggttatc tatgggattt atttttcaaa cacttattaa
    32701 tgtctgtcta tggtgtgctg tttgcagctt taattggtat tccattggga atcttgcttg
    32761 caagatacac aaaactttct ggatttgtaa ttacaattgc aaatataatt caaacagttc
    32821 cagtcattgc aatgttagct attttaatgt tagtcatggg cttaggttca gaaacagtag
    32881 ttttaacagt gtttttatat gcgttacttc caattataaa aaacacttat actggtatag
    32941 ctagtgttga tgcgaatatt aaggatgctg gcaaaggtat gggaatgaca cgcaatcaag
    33001 tgctacgaat gattgaatta ccgttatctg tttcggttat tatcggtggc attcgtattg
    33061 ccttggttgt tgcgataggt gttgttgccg ttggatcatt tataggagca cctacgcttg
    33121 gtgacattgt gattcgtggt acaaatgcga cggatggcac aacgtttatt ttagcaggtg
    33181 cgattccgat tgctatcatt gcaatcgtca ttgatgtact attaagattt ttagaaaaac
    33241 gattagaccc aacaacacga catcgtaaaa atcaatctaa tcatcggccg caaagtatta
    33301 atatgtaata gtagaagatg tttataattt agcgatttcg tttcatgatt tataaaaaat
    33361 gaggctactc aaggagctca aataatcttt gagtagcctt tttataggtt gtgtttgtat
    33421 gcgtttacac taaaatagca attattatca tgaaagtttt tggataaaaa gcgttaatta
    33481 ttgtaaaaat actaaaaaat gagatgtttt atttataatt ttctgcaaat ttatgatatt
    33541 gtttcttaat atatcatatt aaaaatttgt ttttcttaaa cataggaggc ttatctaatt
    33601 catggacaca tcaaaacaat ttagaggtga caaccgattg cttttgggta tcgttttagg
    33661 ggttattacc ttttggctat tcgcgcagtc acttgttaat cttgttgtcc cattacaatc
    33721 aacatatagt agtgacgttg gaacgataaa tatcgctgtt agcttatctg ccttatttgc
    33781 tggtttgttt atcgtaggtg ctggtgatgt tgctgataaa tttggtcgcg tcaaaattac
    33841 ttatgtagga ttgatattaa atgttgtagg ttcattactc atcatcatta cacctttgcc
    33901 agcattttta attataggta gaataattca aggtttgtct gcagcatgta ttatgccatc
    33961 aacacttgct attattaacg aatattatat tggtacaaga agacaacgtg ccttaagcta
    34021 ttggtctatt ggttcttggg gtggtagtgg tatttgtacg ttgtttggtg gcttaatggc
    34081 tacatatata ggttggcgtt caatatttgt tgtttcaatt ctattaacat tattagcaat
    34141 gtacttaatc aaacatgcac ctgagactaa agcagaacca atcaaaggta tgaaagcaga
    34201 agctaaaaag tttgacgtta ttggtttagt cattttagta gtgacgatgt taagtttaaa
    34261 tgtaatcatc acacagacgt ctcattttgg tttagtttca ccgttaattc taggtttaat
    34321 tgttgtgttt atctgttcat tagttggttt tgtatattac gaaaataaaa taaagcatcc
    34381 acttgttgat ttttcaattt ttaaaaatag aggatacagt ggtgcaacta tttcaaactt
    34441 cttattaaat ggtgtagcag gtggtgcact tatcgttatt aacacgtatt atcaacaaca
    34501 attaggattt aattcttcgc aaacgggtta tatttcatta acgtatttaa taacagtgtt
    34561 gtcaatgatt cgtgtaggtg aaaagatttt atctcaacat ggtccgaagc gcccactatt
    34621 actaggaagt ggctttacag tgattgggtt aatcttattg tcgttaacat ttttaccaga
    34681 agtgtggtat atcatatcta gtatagttgg atatttattg tttggtactg gtttaggatt
    34741 atatgctaca ccatcaactg atacagcagt tgctagtgcg ccagatgata agtcgggtgt
    34801 tgcttcaggt gtgtataaaa tggcgtcatc attaggaaat gcatttggag tagcagtatc
    34861 tggtacggtt tatactgtgt tagcagctaa tttaaatttg aacttaggtg gtttcacagg
    34921 tatgatgttt aatgccttgc tagcaattgt tgcattttta gtcattttac tattagttcc
    34981 taaaaatcaa acgaatttgt aaaactgaaa tgaaagcaag ttattatgta gggattttaa
    35041 aggaaatttt gtgaaagtaa gtttatcata cacacttaat gttgcgtatt gacgtttaat
    35101 gttaggtgtg ttcttttata gacgataaaa gctgtgtgca tattaagcga atgattttca
    35161 aattgacgct aatatgcgaa agtagtattt ttaaaatgaa caacaacgat gaagaggggt
    35221 ttataggatg aaaattgcaa ttgctggatc gggtgcatta ggtagtggct ttggtgccaa
    35281 actatttcaa gcaggatatg atgtcacact tattgacgga tatacatctc atgttgaagc
    35341 ggttaagcaa catggattaa atataacgat taatggagag gcattcgagt taaacattcc
    35401 gatgtatcat tttaatgatc aaccggacga aagcatttac gatgttgtct ttctatttcc
    35461 aaagtctatg caattaaaag aagtgatgga agatatgaag ccacatattg ataatgaaac
    35521 gatcgtcgta tgtacgatga atggtctgaa gcatgaagaa gtcattgcgc agtatgttgc
    35581 tcaatcacaa attgtcagag gtgttacgac ttggacggca ggtcttgaaa gccctggaca
    35641 cagtcattta cttggtagtg gaccagttga aataggtgaa ctagtggatg aaggtaaaga
    35701 aaatgttata aaagttgctg atttacttaa cgaagcggaa ttgaatggtg tcattagtaa
    35761 agatttatac caatcgattt ggaaaaagat ttgtgttaat ggtacggcaa atgcattaag
    35821 cacagtgttg gagtgtaata tggcatcgct gaatgaaagt agttatgcga agtgtttgat
    35881 ttataaatta acgcaagaaa tagtgcatgt agcgacgatt gataatgttc atttaaatgt
    35941 tgatgaagta tttgaatatt tagttgattt aaatgaaaaa gttggtgcgc attatccatc
    36001 catgtatcaa gatttaattg ttaataatag aaaaactgaa attgattata ttaatggcgc
    36061 agttgcaaca ttaggtaaac aacgtcatat tgaagcgcca gtcaatcgct ttattactga
    36121 tttaattcat actaaagaaa gtcagcgaca cgcacaggat taattggtgt tttaggtatg
    36181 attacaagga aatgacaatc acgtgatatt acggtcatta ttaagattga aatgtaataa
    36241 ataaagaaca gcagtaaggt actttcaaat tgaaatgatc ttggtgctgt ttttcttgat
    36301 tgatcttcgt cataattcag atttgtcata ggtctacgac atactattag tatttactag
    36361 acagttttta cgacgacact ttgaaaaatt ttgaggcaaa tcatttggaa gtctcacgtg
    36421 aattttgtaa actcatcaag caagtaatta tattaaaaag acaaatagag aaaaggtgtt
    36481 tataatgagt aaaatttttg taactggtgc aacgggcctt attggcatta aattagttca
    36541 aagactaaaa gaagaggggc atgaggttgc tggttttact acatctgaga atggtcaaca
    36601 aaagctagct gctgttaatg taaaagcata tattggtgat atattaaaag ctgatactat
    36661 tgatcaagcg ttagcagatt ttaaaccaga aatcattatc aatcaaatta cggatttaaa
    36721 aaatgttgat atggcagcaa atacgaaagt acgtattgaa ggttctaaaa acctaattga
    36781 tgcggcgaaa aagcatgacg ttaagaaagt aattgcccaa agtattgcct ttatgtatga
    36841 acctggcgaa ggattagcaa atgaggaaac ttcacttgat tttaactcaa ctggcgatag
    36901 aaaagtaacg gttgatggtg tggttggttt agaagaagaa acggctcgta tggatgaata
    36961 cgttgtttta cgttttggct ggttatatgg cccaggtact tggtacggaa aagatggcat
    37021 gatttataat caatttatgg atggtcaagt gacactttca gatggcgtaa catcatttgt
    37081 gcatcttgat gatgcagttg aaacatctat tcaagctatt cattttgaaa atggtatcta
    37141 taatgtagca gatgatgcac ctgttaaagg ttctgaattt gcagaatggt ataaagaaca
    37201 acttggtgtt gaaccaaata ttgatattca acctgcgcaa ccatttgaac gtggcgtaag
    37261 caatgagaag tttaaagcgc aaggtggtac tctgatttat caaacttgga aagatggcat
    37321 gaatccaatt aaataataat ttatccgttt aatatacaaa gaataaagac ttggtcgaat
    37381 cgtggatgat atattatcaa acgcacggct cgaacaagtc ttttttatta tgtcttcgtt
    37441 atctttgtat gaaggaataa cagaattaca attaatgtac tgaataatgc aattaatgtt
    37501 gtgattagtg ctaatttaat ttctattggt agccaagtca gtacaaaaga ccaattattg
    37561 ctaccgagaa tgagatatgg taatgcatat aatatgagcg ctaaagcgat acatatacat
    37621 aatgataacc aactcaatac agcaatccgt tttgatctac gcaaaagaat ttgaccatgg
    37681 cgcatttgcc aagcacgata agctgagaat ataaatgcta ataaaataag tgttgtcatc
    37741 aaaacggtaa caatattaaa ttgatcttta tattgattga gcatagacgc aaccgtcgaa
    37801 tatcgcttgt gatttacaat ttgtgtatta agatgctcaa ctaatttggg tacatattcc
    37861 gaatttagat ttgcaagtac aacaattcca taattttgtt ttggatttag taaaataaat
    37921 gatgaaaagt tatctagcgt tcctgaatga aacactaaat gttcatcatt attggtaaac
    37981 cagccggaag catatgcatt ggcattaggt tcaccaattg ttgaagataa atttttatgt
    38041 gattgttgaa ctaatgattt gtatttatca ggtggattaa gttggaattt tatccaatgt
    38101 tccaaatctt cagttgatgt catcatatat gctgatggtg tatcccaaag gttaaattca
    38161 ggtttagaga cgacaggtgt cgaaccttgt aattcatagc caatagcatc atgttttgat
    38221 ttgtaattgg tttgtttgaa tgatgtatgt gtcatatgca aaggcttgag ccatgaattt
    38281 gtaatatatt ttgtatagga ttgcttcgta acgttttgga taattaaacc taataaatca
    38341 tagttcatat ttgagtattc aaattcttct ccgggcttat gatgtaattc atcacccata
    38401 attgcacggg ttacatcatt taaacgatta tttttgcttg tcacagaatc ttcgcttgta
    38461 atatcactag gtataccact tgtttgagcc aaaagttgct taatcgtaat agtttcattt
    38521 tgaccattat agttcatttt aaaatgaggc acatgtttgg atacggcatc atttaagttt
    38581 aatcgacctt cttgagctaa ttttaaaatt gcaagacctg tgaaagcttt cgtattagaa
    38641 gcaatttcat actttgttgt gggagaagct ttgacttttt tatcaacatc ttgataacca
    38701 taacctttat ttaaaaaaac tttgccattt tttactatta aaattgatgc tccaggaatg
    38761 tgtccctttt gtaaatcatg ctcgataatt gtatctattt gttgttgcga atcattggtt
    38821 aaccgtgtct tcgtattgct atttaatgtt atatatatag caattgaaat ggcgactaaa
    38881 atgataatag aaatggatag aaaatacagt tttttagtag tcataaacgt tcctttttat
    38941 atgttaaatt tacttaagtg cctaatgaga tttattttat tataccagtt ctattgaata
    39001 aattaattaa agaggtatat ttaccattat ttttgacacc ttatatgtta ataggtgtat
    39061 aattacataa aaaatattaa ttaactctct atactttttg gaaagatagg cgtatagtga
    39121 tatccatcaa aaactataaa aaggaatagg agattatgtt caatcaattt aaaagactta
    39181 ttatagggca acctaaaaaa aacagagaac taaaagacga aaagatatcg aagtttaaag
    39241 ggttagcgat tctttcgtcg gatgcattat cttcagtggc ttacggtccc gaacaaatac
    39301 tgattacact atcagtagtg ggtgcagttg caacttggta tactttaccg attgcaggtg
    39361 ctgttctgat cttattagct gctttaatta tgtcttatag gcaaattatt tatgcatatc
    39421 ctaaaggtgg cggcgcgtat atggtgtcca agaccaattt aggagaaaaa tggggattgc
    39481 ttgcaggagg ctcattatta gtcgattaca tactgactgt cgctgttagt atatcatctg
    39541 gtgctgatgc atttgttgct gcattcccaa gtttatatgg gcataaagta ctgattgcat
    39601 gtttacttgt actttttatt ttaatattaa atttacgtgg tttaactgaa tctgccaccg
    39661 tattatccta tccagtatat cttttcatta tagggttagt gatattaata tttataggta
    39721 ctttccgtgt ggcgacagga gatattcaac cacatatgca tgcatcagta ggaactgcgg
    39781 ttcctggagt aacattattc ttgctattaa aagcattttc atcaggggct tcatcgttaa
    39841 cgggtgttga agctatttca aatgcggtaa ccaattttag agagccaagt gctaaaaatg
    39901 ctgtgaaaac gttaattgct atgggttcaa ttttagcgtt tttattagtt ggtatcgttg
    39961 gtttagcata tgtttatgga attttgccac aaacggaaac gacagtttta tcacaattag
    40021 ctatgcaaat atttggtgat aatgctgcct tctattttgt acaggcaacg accgtgatga
    40081 ttttggtatt agcagccaac actggattta cagcttttcc gatgttagcg gcttcaatgt
    40141 ctaaagataa atatatgcct agaatgttta ctgttagagg tgatcgctta ggctactcta
    40201 actcgattat tattttaggt gtattagcca ttattttaat tattgtattt gatggtatga
    40261 ctgaagattt aattccattg tatgcggttg gtgtatttat tccatttaca ctagcacaat
    40321 tcggtatggt tattaaatgg attcatgaac gtcctaagaa ttggttaagt aagttgtctg
    40381 tgaacttact tggtggtatc gtgacattta ttgtttttat gattttactc ataactaaat
    40441 ttagccaagt atggccgata cttattttct tgccgttcgt ggtgattttc ttcttgaaaa
    40501 ttaacaaaca ttatcgagat atcgcagaac aattacgttc tgacattgat gttcttaatg
    40561 tggacgtggt agatcgtaat ttagcaattg tgccgataac aagtattaca acagcagttg
    40621 ataagtctat ttattacgca caaatgcttg cgaataatga tgtcatagca gtacatgtat
    40681 catttggaga tgaagatgaa aaagcattcc aagagaaatg gaaacgtcat ttcccagatg
    40741 taagattagt tattttacat tcggaatatc gcagtattat acgaccaatt tcacgtttta
    40801 tcgataagat taatcgtaaa gccaacgatc aaaactatat gattacagta gtgataccag
    40861 aatttattac taagaaacgt tggcataatt tattacacaa tcaaactagt ttgcgtatga
    40921 aattgtattt aatttatcaa aagaatgtta acgtatgtac aattccattt aaacttaaaa
    40981 aatagattct ataatcaaat cggattgatg aaaggttatt tacttttcat caatctgatt
    41041 ttttgatgtt gaatataaaa accccgtaca tatgagatga taatgacttt gtctcatatg
    41101 tacgggggta ttgttatgtc ttgcttaatg tttggactgt aaagacaaga caatttcgtt
    41161 gtaattgata ttttctcgaa tttgagctgc aacgatattg ttgattttat ttttgcgaat
    41221 taattcatct aaaagatgac gttgtaagta caatgcttcc aatttgtggt tatggaacat
    41281 agcttgcggt atgatttgat tgctgttttc ttgacgttct tttcgacgtt gtgcacgcca
    41341 ctttaatgag cgactcatat catagtaatg attaatgact aagctaacct caagtacatt
    41401 tgtactattt tgttcttctt ttaagcgtaa aataatattg tgattcacaa tacgcataat
    41461 tttttgaaca tctttgaatg aattttcaaa ttcttgcttc ttagattctt tatttaaggc
    41521 tttgtagtgc ttcatcgctt taagtctaag ccataacatc ttgaagaagt ttgcaaattt
    41581 ttcaacaaat gatgcagtac ggtgtgtctc tgttaattcg acaatattgc ggtaattatt
    41641 aatatcctga tatgttgcct tacctttatc aattaaacgc tcaagtgtag atgtttcgat
    41701 tacttttgca atatcttcta aacgtttgag ttcttttgta ttttgattat caggttctga
    41761 atttaataaa aataacaatt ctccatagta ttggtttaat actgggcgat aatttgtatc
    41821 gtttttgtct tttttacttt cgtttttaaa atgctggatc actttttgaa caatgaaaat
    41881 tttggcagat tgataagtca tacctttaaa agtagtatct tcggcagatg gtgtaattaa
    41941 aggtaaaaca atttgcgcta agattaaact aattaatacc atgaaagatg caataaacaa
    42001 taaatcatta cggtattcga atgcttgtcc ttttgtaatg ataaatggta atgtaagtgc
    42061 cattgaaagt gaaattgtac cgtgaatacc acacatggtc ataataaatg cgtaacgcac
    42121 tcgagaaggt ggtgtttcat gtgaatcatg ttcctcgtct agataagatt gtatattttt
    42181 cgggaaataa aaatctttgt accagaaata aacccaaaca aatcgacatg cataaattgc
    42241 aatagcgatt aatatagtga tcactattaa aaatgaaaag ttgtcaggct ctgtttggaa
    42301 aatatctatg actacagtag gaatcataaa acctaataca acaaatacaa aaccatttaa
    42361 ggcataacta aatgtgttcc aaatttgatg gtaattcatt tgtagttccg tttgtgctct
    42421 aattaaacgg tcgcgttcta aaccatggat tagacctgcg attacaactg caatgatacc
    42481 tgaagcatga acttcttctg ctaaaaagta tacgacaaaa ggagttaata attgaataaa
    42541 agttaaggta ttgttatctt ttaaaccttt attagcggtt aagtctatac gtattctaac
    42601 gacaacgaat ccaataattg caccaataag tacacctagt attgttgaaa tgataaattg
    42661 ttcaacagct tggaataatg aaaaggtacc agttactaat gcagtaacag caattttaaa
    42721 tgaaatgata cctgctgcat cattgagtaa agattcacct tctaaaatcg tcatagaacc
    42781 tttaggtaat aattttccgc gtgtaatagc agatactgct actgcatcag taggacataa
    42841 aattgctgct attgcaaaag cggctggcat tggtaaggca ggccaaatcc aatgtataaa
    42901 atagccaaca ccgactacag ttgcaaacac tagtgccatt gacattaata gtataggttt
    42961 acgatattct aataattttg ttcgagagac gtgggtacct tccacaaaaa gtagtggcgc
    43021 gataacggca aacataaata cttcagaatt gaattggaaa tcaacttgta ttggaataat
    43081 gaaaataacg acacctaatg caatttgaat aaaggcagta ggaatttgtg ggaatcgatt
    43141 attgataacc gaactaataa tcacagcaaa aataaaaatt aaaaatgctt ctaatagtgc
    43201 catacaatac tcctcaaaat tttaatagtt aatattttat cacttttaag gcataatgac
    43261 atagatatat tgataaaatg aagttatttt caaaaaaact ctagtatcgg ttgaactgat
    43321 actagagcga gatgtttaaa ttattgattg tcatatctga aatgaccgct gtcattttgt
    43381 cgttgttcat acgcgagctt ttcagcattc gttttgtatt ttttataaaa gaaaaataaa
    43441 aatataaacc agaatggcga aatataaata gctgctcttg tttcgtcact aaagaataat
    43501 aaaatgaata caaagaagaa gaacgctaga ataatgtaag caataggctt accaccaatc
    43561 aacttaaatt tactgttttt atgtgcctca ggatgctttt tcaaatacat tagataagcg
    43621 acaataatca ttgcccaaac aactaaaaac aacacagttg ataacgttgt aacgtatatg
    43681 aatagttgaa ttgcattagg gaaaatgtag tttaacaagg ctgctataag taatgcaatt
    43741 gatgaaacta acattgataa ataaggcacg ccatgactat tcgttttatt taaaacttta
    43801 ggacctaacc cttgttgtga cagtccgaat aagatacgac tattcgaata gataccacta
    43861 tttgtagcag aggccgcggc agttagcacg acaaagttaa cgacacctgc tgcaaatggt
    43921 acgccgatta atgtaaagag tttaacgaaa gggctactat ttggatcgat atcattccaa
    43981 ggtatgactg acataattac taatagacca ccgatataga ataataaaat acggataggt
    44041 acattattaa ttgctttcgg taaggttttt tcaggatctt tcgtttcacc agcagttaca
    44101 cctataagtt caataccaat gaatgaatat acagcaattt ggaatgacat taagaaacca
    44161 aatgttccac cagggaacat gccaccgtga ctgattaagt ttgtgaatga tgcatgtcca
    44221 taatgtgttt taaatgagaa aaagattaat acaagaccaa cgataaccat cgcaataatt
    44281 gtgacaactt taataatcga gaaccaaaac tccagttcac caaataatct ggcacctaat
    44341 aagttgaagc taatcaagat taaaacaata aatagcacgg taatccaatt tgggacttgt
    44401 ggataccaaa atgcaaagta ttgtcccatc gcagttaggt ctgacatact tgatacaatc
    44461 caacataacc agtaagtcca gccaatgaca aagccaccaa aagggcctaa atattcattt
    44521 gcaatatcaa caaatgaatt aaatcttgta ttgctcaaca acaattcgcc taatgcgcgc
    44581 ataaaagcga atagtacaac cccaataatc atgtatgtga ataacagtga tggaccagtt
    44641 aaagatattg tttgacctga tcctaggaat aacccagttc caattgcgcc cccaattgct
    44701 attaattgga tgtgtctgtt attcaactct ctatgcaatt ttcttgccat aattttcctc
    44761 ctcaggataa agcaaataaa aaagttgtgt gtaagcgttt tcttaattat aaatactaaa
    44821 gaaatccatt taataaagca attatttttt taaatataag aaaaaacatt tgataggtaa
    44881 gggtatgata actaaaactt atcgattttt tgaaaagata taagttttta tgcaaaaata
    44941 taaaataatc atctaaatga agcagttaac tatatatatt agataagcta tggcataata
    45001 aataacattt taatttgagt aaagaaggta agggtatgaa gatagggaca atcgccgatt
    45061 tacatattga tcgtcataat aaaaaacatc cgaagattac ttagaggcat tggttgaaat
    45121 agttaagtat aagaaattgg atatattgtt gattgcgggg gatatctcaa atcattatca
    45181 attgacgcac caatttataa cgcaattgac aaaacaatta gatatacctg tgaaatttgt
    45241 acctggaaac catgatttat gggaagttga aagtatgact acgcaagaca tttggaataa
    45301 ttataagagt atgtcacagt gcttggtagg aaaaccattt atagtaaatg aagaatgggc
    45361 aatcatagga catactggct ggtatgatta tagctttgca gcacaacgat tttcattaga
    45421 tgagttacaa aaaggaaaac attatggtgc gacttggcaa gataaagaac gaatatcttg
    45481 gggcatatca gatcaaaatt tatctaaaat agcggctgaa caagtgaaga aagatatatt
    45541 agaagtagga aatagacgag tgattttagt cacacatgtt gtgacgcacc ctgatttcat
    45601 tgttcctatg ccgcatcgta tattcgattt ttataatgca tttattggga caagtgattt
    45661 caatcctttg tatgcgatgt tcgatatacc atatagtatt atgggccatg ttcattttcg
    45721 taaaagtgtg atagatgatg gcagatgtta tctctgtccg tgtctaggct atccaagaca
    45781 atggcgttca gaagatattt accaggaaat aaatgagacg atacaaataa tagaaattta
    45841 aaatgcgcaa acctgaccca gtttgcgcat tttatgtttt acacacgcga gtaatgtgtt
    45901 tacttacgtg tgtttatttt gttgctgatt ttcaattgta tatgaatgtg gttgcacata
    45961 aatgcacttt cttcctggtg aattaaagct gtattccatt ttctctttac gaattttaat
    46021 aatttgtttg cgatttggaa tgatggcagg taaaactagg ccacgacgaa tatgactcca
    46081 aagtggatcg atataatagt aatagtaatc atcgtaccca atgagtaacg ttacgtgaat
    46141 atttgaaact tgtttcgctg gcttgttatc aaagcggaaa acacgtagta atggtttaga
    46201 accaagatta gtatggtata ttaacacagg ttgaccttga tcgataatac ctttaagatc
    46261 ttctaacgat ttaccagtgc cgtctacgat attaggattg tatttttgta aaaatggtac
    46321 atatgcttct ggaaatatcg tttgatgata attgccaagc ttaatgaata agtgatgtcc
    46381 aacataacct ttatgtggat tgttcggatg tgtcggccaa tgtctcataa tttctgtagc
    46441 agggatatgt tggttgttgt attgcaacat catggctgcg gaaacacctt cacaccccat
    46501 gaccataggg ataggaaata gctgactgat aggtttaact ggtaatattt ttcggttcat
    46561 aatatagtcc tcgcattgat tcaataaata tttaatataa ttatatagcg tcaatgcaaa
    46621 atgtcctaaa catatgtttt acatgagtga ataaaattaa tggagtgata aaatggaata
    46681 tcaattacaa caacttgcgt cgttaacgtt agtaggtatt aaagaaacgt atgaaaatgg
    46741 acgacaggct cagcaacata tagcagggtt ttggcaaaga tgttatcaag agggagtaat
    46801 tgcggattta cagttaaaaa ataatggtga tttagccggg atacttggct tatgtatacc
    46861 tgaattagac ggtaagatgt catatatgat tgcagttacc ggagataata gtgctgatat
    46921 tgaaaaatat gatgtcataa cattagcaag ttcaaagtat atggtatttg aagcacaggg
    46981 cgcagtacct aaagcagttc aacaaaaaat ggaagaggtt catcactaca tacatcaata
    47041 tcaagcagat acggtaaaat cagcaccatt ttttgagttg tatcaggatg gtgatactac
    47101 aagtgaaaaa tatattacag aaatttggat gcctgttaaa gggtgattga atagaaatgt
    47161 ccttgctatg aaggcgtaat tttagttgaa gattcgatct cataattcat aaagaagaac
    47221 cctgacaaag atagatatct ttgctagggt tctaattatg ttgaagtgtt atgcgacatg
    47281 ttgcttttta atcattttaa agtaaatgat ttgtagactg actaatagaa tggctgtaat
    47341 gaagataata atcatatatg gtgatgtatt aaattcgcct tttaagccaa ctaatggtgc
    47401 aacagcgcca cctaagataa attggaataa gccaagtaaa cttgatgcgt taccactgcc
    47461 acctgttcgt tcttccatag ccattgtgaa accaagcggt ccaattgacg tcacaggaca
    47521 cacatttaag aagaatgcga tgagtaagac ccaaagtggt aaatggaatg taagtgtcag
    47581 gataattaaa gcaacaccta ccacttgtat aatagttaaa atgattaata atatgtggcg
    47641 atgtaatttt tctactaata aagcaacgac ttgactgaca atgattaaac ctacaccgtt
    47701 aacagcaaac atgatactaa attgttgggg tgtcatatta tacaattttt gagtaataaa
    47761 tggcgatgca gatgaataac taaatagcat tacataagtt aaaccttgta atagcattgg
    47821 aataataaat gctggttttt tcaaaagact tccaaaatct ttaatgacgc tactaaaatt
    47881 cacctgcttt aattcatctt tagatgtttt aggtaattga gaagcgacgc caattaaaat
    47941 gatgagtgcc acaatagtta aaattgtgaa aatagaacgc catgttgcta cggataaagc
    48001 taatccaccg gctaatggtg caagaatagt gatgatgcca ttaactacca ttaaagatgc
    48061 taaaaattta gcgagtgcgt tgccactaaa tttatcacca gcagaggctt ttgcaatcac
    48121 gatgacgcca ccaccagtta aaccttgaat aaatcgtaaa gttaagaata atggcaattg
    48181 atcaacaaac atagacaaac ctgataccaa aatgaaaatg atcaaaatgg atacagcaat
    48241 ccgtttgcga ccaaaagcat cggataaagg tccaaagata aattggccta gtgcaagacc
    48301 aatcattgtg aatgataatg ttaattgaat ttctgatgta gtagaaccaa aatcaccttg
    48361 cactttaggt aatgatggta cgtacatatc tatagttagt gggccaaaag cggtcatgac
    48421 gcctaacgta attagcaata aaaatgaaaa atgcttcgtt ttggttttcg ttgattgcat
    48481 gatcagactc ctttaaatga tataattata taaattatat acgttttgtg tgtatttgta
    48541 tgtacttaga atacaagttt atataaaaaa tccctatact cttaatgcat catcacgaat
    48601 aacgtagtga tacagataaa gtatagggaa ttaatgttta tcatcaaagc aggtgtaata
    48661 attaattagc gccaccattg tcatctttaa ataagctttc taatttagat ttaatactgt
    48721 cgaattttgt attaatatca tttaacatgc tttcaatttt accttggctt gcattgtttt
    48781 taagtgcatt atttgcctct gtcaatttgt tgatttggtc ttgataaaat tgagttagct
    48841 tagaatcagc tttgttttgt tcttgctgct tcaagttgtc gatttgattt tgtaaatttt
    48901 taataatgtc atcagttttt tggttgtttt taatattttc tttagcatta tctacttgct
    48961 ggttaatttg gccgtcttga ttttcaactt gttttttata attttgagag actttagggt
    49021 cattactaac tttatcgcta tcattgttaa gtaaggcgcg tgtgacaaaa atagcgacaa
    49081 taattaagag aatggcaata atgatatata accatttttt tgaacggcgg gatttctcgt
    49141 tttcttcttc aagaatttgt tctcttcgat aatctctacc attatagtat cgttccgggt
    49201 gttcttcagg atcgccttta gcatattgtt gtctatacga tgcgtcttca gattgacgac
    49261 gatgattttg gttttgattg tcgtattgat atgaatcacg gtatttatca gttcttttca
    49321 tgggtttcac ctcattcaag ttttaactgc aaataataaa gctgatgatt tactttgaca
    49381 ttattgtaat ttcagaatac gagcaatgtc agttgcagtg tttaataaat tttgttcgct
    49441 attttgtagg acatcttcta aagtgctagg tgaagagatt atagaatagg cactatcaat
    49501 accgaaatcg taaacatgtt gataattttc gcctagactg ccacaaatcg cgatgacagg
    49561 aatatgatat tgttttgcag ctaacgcaac gcctacgggt gttttaccaa agatggtctg
    49621 ataatccatg cgtccttctc cagtaataac gaggtctgca tctttaattc tttgatgaaa
    49681 atctgtaatg tcaaagacga catcaatacc ttttgttaaa gttgtctcac aaaacgctaa
    49741 taatgctgcg cccataccgc cagctgcacc agaacctggt atttgattaa cggactttcc
    49801 tgtgcacatt tttatcttat catgataatg cgacattgcg aaatccaact ttggtatcat
    49861 ctttgcatca gcgccttttt gaggaccata aatataggta gcaccatttt cacccaataa
    49921 aggatttgaa acatcacagg ccactttaaa ggtcacctct tttaatcgcg aatctagatt
    49981 ggttatatcg atttgtgcaa tgtgagcaag attagcacca ttcatttgta ataagtcccc
    50041 gtttacatca gtaaacttta cgcctagtgc acttagcata cctgtaccac catcatttgt
    50101 tgcactgcca ccaatcccta aaataatggt cttagcacca tgatttaatg catctttaat
    50161 tagttcaccg gtaccatatg atgatgtgta taaaggatta cgttcctctt tttctaataa
    50221 atccaaacct gacgctgccg ccatttcaat aattgcaatt tgttgttcgt ctgcgcgtgc
    50281 ataacatgct tcaataggtc gcattaaagg gtcattaacg atgactgtat acttagtggc
    50341 acctgttgca tgcattaaag catctgtggt accttcacca ccatcagcca tcggaatgat
    50401 atcataatga agggtattcc cataaacatt agtaaaagcc tgttttataa tattgccaac
    50461 ttgctgtgcg gtcatacttt ccttaaatga gtcaggagcg atgacaattt tttgtaattg
    50521 catagtagac atcctctcat tactgttgtc gctcttattg tatataaaat tactagtgaa
    50581 ctaaagtaat ctctatagtg ctaatgagtc acgcgataaa tcagttacat tataatggcg
    50641 tataaggatg aggggaaaga gactaaattt gctgtgaaag ggtataaaga ttgaaataag
    50701 catatttgaa gtgaagcgga ttggctcgca tattcgagta tgttcgagaa cgtagttatg
    50761 catatatttt gatgttgagg agttacacat atgaaactaa cacaaacgca tgctgaaatt
    50821 ttaaaattta tcattgttgg cggcattaat acgttaaatt attatgttgt ctatttattg
    50881 ctgttaaaat tattacacat tgaatatatg attagtcata ttaccggatt tctagtcgct
    50941 tttgtgattt catattattt gaattgttat tttgtttaca gggtaaaacc tacttggaga
    51001 aaattcatta gttttccaat tacgcagatt gtcaacgtaa gcttacaaac agttttatta
    51061 tatgtatttg tatcctggct aaatttgcca gcagaaattg caccatttgc tggtttaatt
    51121 attacgatac ccatcacatt tatattatct aaatggattt taaaagatag caattaaagt
    51181 agcgtaaacc actaaaaaaa actttactta agccttaata ttgcgtgaaa tataataaat
    51241 aaatgatata atatgatggt cgcctaacat ctcgggcgtt actttaacgc agagaggagg
    51301 tgtatgacgg atgttcaatt tattaattaa catcatgact tcagctataa gcggctgtct
    51361 tgttgcgttt tttgcacatt ggttacgaac gcgcaacaat aaaaaaggtg acaaataacc
    51421 aacacctaaa taaatcccct cactatggca gtag
//
LOCUS       DAKTGS010000006        42233 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.006, whole genome shotgun sequence.
ACCESSION   DAKTGS010000006 DAKTGS010000000
VERSION     DAKTGS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 42233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.006"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..862
                     /locus_tag="O0286_000288"
     CDS             <1..862
                     /locus_tag="O0286_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCY0168690.1"
                     /translation="LVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAI
                     HEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQFGFVNIG
                     VKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIYNRYTLH
                     RFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDAL
                     SAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELH
                     VSGKYISSHF"
     gene            1337..2455
                     /gene="ald"
                     /locus_tag="O0286_000289"
     CDS             1337..2455
                     /gene="ald"
                     /locus_tag="O0286_000289"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0168691.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            2550..3590
                     /gene="tdcB"
                     /locus_tag="O0286_000290"
     CDS             2550..3590
                     /gene="tdcB"
                     /locus_tag="O0286_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCY0168692.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            3621..4943
                     /locus_tag="O0286_000291"
     CDS             3621..4943
                     /locus_tag="O0286_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0168693.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            5100..6491
                     /gene="norB"
                     /locus_tag="O0286_000292"
     CDS             5100..6491
                     /gene="norB"
                     /locus_tag="O0286_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCY0168694.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            6888..38153
                     /gene="ebh"
                     /locus_tag="O0286_000293"
     CDS             6888..38153
                     /gene="ebh"
                     /locus_tag="O0286_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCY0168695.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(38212..38613)
                     /locus_tag="O0286_000294"
     CDS             complement(38212..38613)
                     /locus_tag="O0286_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCY0168696.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(39154..39858)
                     /locus_tag="O0286_000295"
     CDS             complement(39154..39858)
                     /locus_tag="O0286_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCY0168697.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            40103..40297
                     /locus_tag="O0286_000296"
     CDS             40103..40297
                     /locus_tag="O0286_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCY0168698.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            40309..40560
                     /locus_tag="O0286_000297"
     CDS             40309..40560
                     /locus_tag="O0286_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCY0168699.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            40598..41722
                     /locus_tag="O0286_000298"
     CDS             40598..41722
                     /locus_tag="O0286_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCY0168700.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            41738..42175
                     /locus_tag="O0286_000299"
     CDS             41738..42175
                     /locus_tag="O0286_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0168701.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT        16632 a         7244 c         7622 g        10735 t
ORIGIN      
        1 acttgtagat ggtatggcgc tattatttag acatttctat gctacaagtc ttcataaaca
       61 atttatgtac aattcacaag gtgaacctac aaatggaata caaggatttg tgcgtcatat
      121 cttttcggca atacatgaaa tacgccctac acatgtagct gtatgttggg atatgggaca
      181 atcaactttt agaaatgata tgtttgatgg ttataagcaa aatcgttctg caccaccaga
      241 agaattgata ccacaatttg attatgttaa agaaatttca gagcaatttg gctttgtaaa
      301 tattggcgtt aaaaactatg aagcggatga tgttataggt acattagcac aacaatattc
      361 aactgataac gatgtctata ttattacggg cgacaaagat ttactgcaat gtattaatga
      421 caatgttgaa gtttggctaa ttaaaaaagg ttttaacatt tataatagat atacattaca
      481 tcgttttaac gaagaatatg cccttgaacc acaacaacta atcgatatta aagcgtttat
      541 gggtgataca gcagatggat atgcgggtgt taaaggtatt ggcgagaaaa cggctattaa
      601 gttaattcag caatatcaaa gcgttgaaaa tgtggttgaa aacattgatg cattatcagc
      661 tgggcaacgt aataagatta atgacaattt agatgaacta tatttatcga agcgactggc
      721 tgaaattcac acacaagtac caattgatag tgaagcgctt tttgagaaaa tgtcatttgc
      781 aacaacgctt aatcatattt tatcaatttg taacgaacat gaattacatg tttcaggaaa
      841 atatatttca tcccattttt aaaaagtata agattttatg aaaaattaaa ccaattaagt
      901 ataaaaagac tatttctatc gatacaaggt agaaatagtc tttttgcgta tttggatatg
      961 tcagagtgaa taaataagcc gaattttgat agatacatat ttgattaatc aatagagtat
     1021 aaagaattat atttcatcca aataaattac catacactac aaaatagtac agttacaact
     1081 ttattaatct aaaaaacata tttgattaat tagtttttga gtcaatgtat taatacaact
     1141 ataaatcaaa tggagtataa gaaataaata tagtggtata attgcacata atatttaaga
     1201 ataagaataa tatattaaat atttattaac aaattataga taaaatatga ataattaatt
     1261 aatggtattt acatattcat aaccaaagga taaaataatt gtgaaattat tcacaaatga
     1321 aagaggagtg ttgcaaatgt tagttgcagt agtcaaagaa ttaaaacaag gtgaaggacg
     1381 tgtagcttgc acacccgaaa atgtgcgtaa gttaacggat gcaggacata aagtaattgt
     1441 tgaaaaaaat gctggcattg gttcaggatt ttctaacgat atgtatgaaa aagaaggcgc
     1501 taagatcgta actcacgaac aagcatggga agctgatctt gttatcaaag taaaagaacc
     1561 tcatgaaagc gaatatcaat atttcaaaaa gaatcaaatt atctggggat ttttacatct
     1621 agcatcttca aaagaaatag tagaaaaaat gcaagaagtt ggtgtaactg cgattagtgg
     1681 tgaaaccatt ataaaaaatg gaaaagcaga attattagcg ccaatgagtg ctatagcagg
     1741 tcaacgctca gcaattatgg gagcttacta ctctgaagca caacatggtg gtcaaggtac
     1801 tttagtgact ggtgtacatg aaaatgtgga tatacctggt agtacatatg tgattttcgg
     1861 tggtggagta gcagcaacaa atgcagcaaa tgttgccttg ggactaaatg ctaaagtaat
     1921 cattatcgag ttaaacgatg accgcattaa atatcttgaa gatatgtatg cagaaaaaga
     1981 tgtcacagta gtcaaatcaa caccagaaaa tttagcagaa caaattaaga aagcagatgt
     2041 atttatttct acaattttaa ttccaggtgc gaaaccgcca aaattggtta ctcgtgagat
     2101 ggttaaatca atgaaaaaag gttcagtatt aatcgatata gctattgacc aaggtggaac
     2161 tattgaaaca attagaccaa ctacaatttc tgatccagtg tatgaagaag aaggtgtgat
     2221 tcattatggt gtaccaaatc aaccaggagc agtcccaaga acttcaacaa tggcattagc
     2281 acaaggaaat attgattata tattagaaat ttgtgacaaa ggcttagaac aagcaattaa
     2341 agataatgaa gccttaagta ctggtgtaaa catttaccaa ggacaagtga caaatcaagg
     2401 attagcttca tcacatgacc tagattataa agaaatatta aatgttatcg aatagatagt
     2461 aatttaaatg aaattgagtg aaatgaatat tttaaatata gcattatagt ttggactaaa
     2521 aatttacaaa acggaaggat gtaattatta tgacaaccaa cacagttaca ttacaaacag
     2581 cacatattgt aagtttagga gatatcgaag aagctaaagc aagcattaaa ccatttattc
     2641 gtcgaacacc tctaattaaa tcaatgtatt taagccaaag tataactaaa gggaatgtat
     2701 ttctaaaatt agaaaatatg caattcacag gatcttttaa atttagaggc gctagcaata
     2761 aaattaatca cttaacagat gaacaaaaag aaaaaggcat tatcgcagca tctgctggga
     2821 accatgcaca aggtgttgct ttaacagcta aattattagg cattgatgca acgattgtaa
     2881 tgcctgaaac agcaccacaa gcgaaacaac aagcaacaaa aggctatggg gcaaaggtta
     2941 ttttaaaagg taaaaacttt aacgaaacta gactttatat ggaagaatta gcgaaagaaa
     3001 atggcatgac aatcgttcat ccatatgacg ataagtttgt aatggcaggc caaggaacaa
     3061 ttggtttaga aattttagat gatatttgga atgtgaatac agtcatcgta ccagttggcg
     3121 gtggaggatt aattgcaggt attgccaccg cattaaaatc atttaaccct tcaattcata
     3181 ttatcggtgt tcaatctgag aatgttcatg gtatggctga gtctttctat aagagagatt
     3241 taactgaaca tcgagtggat agcacaatag cagatggttg tgatgtaaaa gttcctggtg
     3301 aacaaacata tgaagtagtt aaacatttag tagatgaatt tattcttgtt actgaagaag
     3361 aaattgaaca tgctatgaaa gatttaatgc agcgtgccaa aattattact gaaggtgcag
     3421 gcgcattacc aacagctgca attttaagtg gaaaaataaa caataaatgg cttgaagata
     3481 aaaatgttgt tgcattagtt tcaggcggga atgttgactt aactagagtt tcaggtgtca
     3541 ttgaacatgg actgaatatt gcagatacaa gcaagggtgt ggtaggttaa aacatttaat
     3601 cttaaaaatg aggtgtaatt atgtcaaatg gtaaagaatt acaaaaaaat ataggtttct
     3661 tctcagcgtt tgctattgtt atggggacag ttattggttc aggagtattc tttaaaatat
     3721 caaacgtaac agaagtaaca ggaacagcag gaatggcctt gtttgtatgg ttcctaggcg
     3781 gcatcattac catttgtgcg gggttaacag cagcagaact tgctgctgca atccctgaaa
     3841 caggtggctt aacgaagtat atagaatata catacggtga tttctggggc ttcctatcag
     3901 gttgggcgca atcatttatt tattttccag ctaacgtagc agcattgtct atcgtatttg
     3961 cgacacagct aattaattta ttccatttat ctataggttc gttaatacca atagcaatcg
     4021 catctgcgtt atctattgtg ttgataaatt tcctaggttc aaaagcaggc ggaattttac
     4081 aatcagttac tttagtaatt aaactgattc caatcatcgt tattgtaatt tttggtattt
     4141 ttcaatctgg agatatcact ttttcattaa ttccaactac aggtaattca ggaaatggct
     4201 tctttacagc aattggtagt ggtttattag caactatgtt tgcatatgat ggttggattc
     4261 atgtaggaaa tgttgcgggg gaacttaaaa atcctaaacg cgatttacct ttagcgattt
     4321 cagttggtat cggttgtatt atggctgtgt atttattaat taacgctaca ttcttattaa
     4381 cgttgccaat agaactactt gctggtaatt taaatgcagc ttcagataca tcaaaaatat
     4441 tatttggtga aaatggcggt aagattatta caatcggtat attaatttct gtttatggta
     4501 cgatcaatgg ctatactatg actggtatgc gcgtaccata tgcaatggct gaaagaaaat
     4561 tattgccatt tagccattta ttcgcaaaat taacaaaatc tggcgcacca tggtttggcg
     4621 caattataca acttataatc gctatcatca tgatgtcaat gggagcattt gatacaatta
     4681 caaatatgtt aatctttgtt atttggttgt tctattgtat gtcatttgtt gcggtaataa
     4741 ttttaagaaa acgtgaacca aatatggaac gaccatataa agtaccgtta tatccgatca
     4801 tacctttaat tgctattttg gcaggatcat ttgtattaat taatacactg tttacacaat
     4861 ttatattagc aatcattgga attctaataa cagcacttgg tataccagtt tattactata
     4921 aaaagaaaca aaaagcagca taaggtaaga taactagcat tgagaataaa tggatggact
     4981 actaataaat ttaaagtttt acacattaaa atcaaaaacc attcaattat tctatggaac
     5041 agacaaattt ctgttatgga atttgtctgt ttttcaaaag tatagggagg caaatagaga
     5101 tggaaaagcc gtcaagagag gcatttgaag gcaataataa gttgttaata ggaattgttc
     5161 taagtgtaat aacgttttgg ctatttgcac aatcattggt taatgttgta ccaatacttg
     5221 aagatagttt caatacagat attggaacgg ttaatatcgc cgttagtata actgctttat
     5281 tttcaggaat gtttgtagta ggagcaggtg gtcttgctga taaatatggc agaattaaac
     5341 tcacgaacat tggtattatc ttaaatatat taggttcatt attaatcatt atttcaaata
     5401 ttcctttatt acttattata ggaagattaa ttcaaggact ttcagcagca tgtattatgc
     5461 ctgcaacttt gtctattatt aagtcatatt acattgggaa agatagacaa cgcgctttaa
     5521 gttattggtc aattggctca tggggcggct ctggtgtttg ttcatttttt ggaggtgcag
     5581 ttgcaacgct tttaggttgg cgttggattt tcatcctatc aattataatt tcattaattg
     5641 cactgtttct tattaaaggc acacctgaaa ctaaatctaa atcgatttct ctaaataaat
     5701 ttgacattaa aggtctggtt cttttagtca ttatgctcct cagtttaaat attttaatta
     5761 ctaaaggatc agaattaggt gtaacctcac ttctttttat tactttatta gctattgcaa
     5821 ttggatcttt tagtttattt atagttcttg aaaagcgtgc tacaaatcct ttaatcgatt
     5881 ttaaattatt taaaaataaa gcttacacag gtgcaacagc ttcaaacttt ttgttaaatg
     5941 gtgttgcagg aacattaata gtagccaaca catttgttca aagaggttta ggatattctt
     6001 cattgcaagc aggaagttta tcaatcactt atttagtaat ggtactaatt atgattcgtg
     6061 ttggtgaaaa gttacttcaa acactcggat gcaagaaacc aatgttaatt ggaacaggag
     6121 ttcttattgt cggagaatgt ctcatttcat taactttctt gccagaaata ttctatgtca
     6181 tttgttgtat tataggttat ttattctttg gtttaggact agggatatat gctacaccat
     6241 caacagatac agcaattgca aatgcaccgt tagaaaaagt aggcgttgct gcaggtatct
     6301 ataaaatggc ttctgcatta ggtggagcat ttggcgtcgc attgagtggt gcagtatatg
     6361 caatcgtatc aaatatgaca aacatttata caggtgcaat gattgcatta tggttaaatg
     6421 caggtatggg aatattatca ttcgttatca ttttgttact tgtgcctaaa caaaacgata
     6481 ctcaattatg ataattgaga attaaattga aatcatacaa gtcgctacaa tattaaacaa
     6541 aaatataaac cgattcttat gtgtcattat tttaaatgaa catagggatt ggttttttat
     6601 tactctttta cgctacttta tttataatta ttataaattg tcacaaattc aatttacctt
     6661 acaatatatt ttgtgttatt atattctgga gcataaataa attgttcaac acatagttgt
     6721 aatgtgtttc aatacttttg gatagattgc gaaattgtat tgaatcgtca tcgttttaaa
     6781 tttttaaatg agaatggaat gagcattaca atacacaagc aatcaaaagt aaatacattc
     6841 acaacacaac agagacataa caacaagata aggagtgaac aatagctgtg aattatcgtg
     6901 ataaaattca aaagtttagt attcgtaaat atacagttgg tacattttca actgtcattg
     6961 cgacattggt atttttagga ttcaatacat cacaagcaca tgctgctgaa acaaatcaac
     7021 cagcaagcgt ggttaaacag aaacaacaaa gtaataatga acagactgag aatcgagaat
     7081 ctcaagtaca aaattctcaa aattcacaaa atggtcaatc attatctgct actcatgaaa
     7141 atgagcaacc aaatattagt caagctaatt tagtagatca aaaagtagcg caatcatcta
     7201 ctactaatga tgaacaacca gcatctcaaa atgtaaatac aaagaaagat tcggcaacgg
     7261 ctgcgacaac acaaccagat aaagaacaaa gtaagcataa acaaaacgaa agtcaatctg
     7321 ctaataaaaa tggaaacgac aatagagcgg ctcatgtaga aaatcatgaa gcaaatgtag
     7381 taacagcttc agattcatct gataatggta acgtacaaca tgaccgaaat gaattacaag
     7441 cgttttttga tgcaaattat catgattatc gctttattga ccgtgaaaat gcagattctg
     7501 gcacatttaa ctatgtaaaa ggcatttttg ataagattaa tacgttatta ggcagtaatg
     7561 atccaataaa caataaagac ttgcaacttg catacaaaga attggaacaa gctgttgctt
     7621 taattcgtac aatgcctcaa cgtcaacaga ctagccgacg ttcaaataga attcaaacgc
     7681 gttcggttga gtcaagagct gcagagccta gatcagtatc agactatcaa aatgcaaatt
     7741 catcatatta tgttgaaaat gctaatgatg gttcgggcta tcctgttggt acatatatca
     7801 atgcttctag taaaggggcg ccatataatt taccaactac accatggaat acattgaagg
     7861 cctctgactc aaaggaaatt gctcttatga cagcgaaaca aactggagac gggtaccaat
     7921 gggttattaa gtttaataaa ggacatgctc cacatcaaaa tatgatcttt tggtttgcat
     7981 taccagcaga ccaagtgcca gtaggaagaa ctgactttgt aacagttaat tcagatggaa
     8041 caaatgtaca atggagtcat ggagcaggag caggtgcaaa taaaccactt caacaaatgt
     8101 gggaatatgg agtaaatgat cctcatcgtt cacatgactt taaaataaga aatagaagtg
     8161 gccaagtaat atatgactgg ccaactgtcc atatttattc tttagaagat ttatctagag
     8221 cgagtgatta ttttagtgaa gctggagcga cacctgctac taaagctttt ggtagacaaa
     8281 attttgaata tattaatggt caaaaacctg ctgaatcacc gggtgttcct aaagtttata
     8341 ctttcatcgg tcaaggtgat gcaagttata caatttcatt taaaacacaa ggtccaactg
     8401 ttaataaatt gtactatgca gcaggtgggc gtgctttaga gtacaatcaa ttatttatgt
     8461 acagtcaact atacgtcgaa tcaacgcaag accatcaaca acgtcttaat ggtttaagac
     8521 aagtggttaa tcgtacatat cgcataggta caactaaacg tgtagaagtg agtcaaggaa
     8581 atgtacaaac gaaaaaggta ttagaaagta caaacctaaa tatagatgat tttgttgatg
     8641 atcctttaag ttatgttaag acgccgagta ataaagtgtt aggattttat tcgaataatg
     8701 caaatactaa tgcttttaga ccgggtggag cccaacaatt aaatgaatat caattaagtc
     8761 aattatttac tgatcaaaaa ttacaagaag cagcaagaac tagaaaccca ataagattaa
     8821 tgattggttt cgactatcct gatgcttatg gtaatagtga aactttagtt cctgttaact
     8881 taacggtatt acctgaaatc caacataata ttaaattctt taaaaatgac gatactcaaa
     8941 atattgctga aaaaccattt tcaaaacaag ctgggcatcc agttttctat gtatatgcag
     9001 gtaaccaagg gaatgcttcc gtgaatttag gtggtagcgt aacatctatt caaccattac
     9061 gtattaattt aacaagtaat gagaatttta cagataaaga ttggcaaatt acaggtattc
     9121 cgcgtacatt acacattgaa aactcgacaa atagacctaa taatgccaga gaacgcaata
     9181 ttgaacttgt tggtaactta ttaccagggg attactttgg aacgatacgt tttggacgta
     9241 aagaacaatt attcgaaatt cgtgttaaac cacatacacc aacaattaca acgacagctg
     9301 agcaattaag aggtacagca ttacaaaaag tgcctgttaa tatttcggga ataccgttgg
     9361 atccatcggc attggtttat ttagttgcac caacaaatca aactacgaat ggtggtagtg
     9421 aggcagatca aataccatct ggttatacga tacttgcgac tggtacacct gatggggtgc
     9481 ataatacaat tactatacga ccgcaagatt atgttgtatt cataccacct gtaggtaaac
     9541 aaattagagc agtagtttat tataataaag tagttgcatc taatatgagt aatgctgtta
     9601 ctattttgcc agatgacatt ccaccaacaa tcaataatcc tgttggaata aatgccaaat
     9661 actatcgagg cgacgaagtc aactttacaa tgggtgtctc tgatagacat tctggtataa
     9721 aaaatacaac tattacgaca ttgccgaatg gttggacatc aaatttaaca aaagcggata
     9781 agaataatgg ctcattatct attacaggta gagtgagtat gaatcaggca tttaacagtg
     9841 atattacatt taaagtgtca gcgacagaca atgtcaataa tacgacaaat gatagtcaat
     9901 ctaaacatgt ttcaattcat gtaggtaaaa ttagtgaaga tgctcatccg attgtattag
     9961 gaaatactga gaaagttgta gtagtcaatc cgactgctgt atctaatgat gaaaagcaaa
    10021 gcataattac tgcctttatg aataaaaacc aaaatataag aggatattta gcatcaactg
    10081 atccagtaac tgtcgataat aatggtaatg tcacattaca ttaccgtgat ggctcatcga
    10141 caacgcttga tgctacaaat gtgatgacat acgaaccagt tgtgaaacct gaataccaaa
    10201 ctgtcaatgc tgctaaaaca gcaacggtaa cgattgctaa aggacaatca tttagtattg
    10261 gtgatattaa acaatatttt actttaagta atggacaacc tattccaagt ggcacattta
    10321 caaatattac atctgataga actattccaa ctgcacaaga agttagtcaa atgaacgcag
    10381 gcacgcagtt ataccatata actgctacaa atgcgtatca taaagatagt gaagacttct
    10441 atattagttt gaaaatcatc gatgtgaaac aaccagaagg cgatcaacgt gtatatcgta
    10501 catcaacata tgatttaact actgatgaaa tctcaaaagt aaaacaagca tttattaatg
    10561 caaatagaga tgtaattacg cttgccgaag gtgatatttc agttacaaat acacctaatg
    10621 gtgctaatgt aagtactatt acagtaaata ttaataaagg tcgattaacg aaatcattcg
    10681 cgtcaaacct agctaatatg aatttcttgc gttgggttaa tttcccacaa gattatacag
    10741 tgacatggac gaatgcaaaa attgcaaaca gaccaacaga tggtggttta tcatggtctg
    10801 atgaccataa atctttaatt tatcgttatg atgctacatt aggtactcaa attacgacga
    10861 atgatatttt aacaatgtta aaagcaacaa ctacagtgcc tggattgcga aataacatta
    10921 ctggtaatga aaaatcacaa gcagaagctg gcggaagacc taactttaga acgactggtt
    10981 attcacaatc aaatgcgaca actgatggtc aacgtcaatt tacgttgaat ggtcaagtga
    11041 ttcaagtgtt agacatcatc aacccttcaa acggttatgg tgggcaacct gttacaaatt
    11101 caaatactcg tgcaaaccat agtaactcaa ctgttgttaa cgtaaacgaa ccggcagcta
    11161 atggtgctgg cgcatttaca attgaccacg ttgtaaaaag taattctaca cataatgcaa
    11221 gtgatgcagt ttataaagca cagttatact taacgccata tggtccaaaa caatatgttg
    11281 aacatttaaa tcaaaataca ggaaatacta ctgacgctat taacatttat tttgtaccaa
    11341 gtgacttagt gaatccaaca atttcagtag gtaattacac taatcatcaa gtgttctcag
    11401 gtgaaacatt tacaaatact attacagcga atgataactt tggtgtgcaa tctgtaactg
    11461 taccaaatac atcacaaatt acaggtactg ttgataataa ccatcaacat gtttctgcaa
    11521 cggcaccaaa tgtgacatca gcaactaata agacaatcaa tttattagca actgatacaa
    11581 gcggcaatac agctacaact tcgttcaatg taacagtgaa acctttgcgt gataaatatc
    11641 gagttggtac ttcatcaacg gctgctaatc ctgtgagaat tgccaatatt tcgaataatg
    11701 cgacagtatc acaagctgat caaacgacaa ttattaattc gttaacgttt actgaaacag
    11761 taccaaatag aagttatgca agagcaagtg cgaatgaaat cactagtaaa acagttagta
    11821 atgtcagtcg tactggaaat aatgccaatg tcacagtaac tgttacttat caagatggaa
    11881 caacatcaac agtgactgta cctgtaaagc atgtcattcc agaaatcgtt gcacattcgc
    11941 attacactgt acaaggccaa gacttcccag caggtaatgg ttctagtgca tcagattact
    12001 ttaagttatc taatggtagt gacattgcag atgcaactat tacatgggta agtggacaag
    12061 cgccaaataa agataataca cgtattggtg aagatataac tgtaactgca catatcttaa
    12121 ttgatggcga aacaacgccg attacgaaaa cagcaacata taaagtagta agaactgtac
    12181 cgaaacatgt ctttgaaaca gccagaggtg ttttataccc aggtgtttca gatatgtatg
    12241 atgcgaaaca atatgttaag ccagtaaata attcttggtc gacaaatgcg caacatatga
    12301 atttccaatt tgttggaaca tatggtccta acaaagatgt tgtaggcata tctactcgtc
    12361 ttattagagt gacatatgat aatagacaaa cagaagattt aactatttta tctaaagtta
    12421 aacctgaccc acctagaatt gacgcaaact ctgtgacata taaagcaggt cttacaaacc
    12481 aagaaattaa agttaataac gtattaaata actcgtcagt aaaattattt aaagcagata
    12541 atacaccatt aaatgtcaca aatattactc atggtagcgg ttttagttcg gttgtgacag
    12601 taagtgacgc gttaccaaat ggcggaatta aagcaaaatc ttcaatttca atgaacaatg
    12661 tgacgtatac gacgcaagac gaacatggtc aagttgttac agtaacaaga aatgaatctg
    12721 ttgattcaaa tgacagtgca acagtaacag tgacaccaca attacaagca actactgaag
    12781 gcgctgtatt tattaaaggt ggcgacggtt ttgatttcgg acacgtagaa agatttattc
    12841 aaaacccgcc acatggggca acggttgcat ggcatgatag tccagataca tggaagaata
    12901 cagtcggtaa cactcataaa actgcggttg taacattacc taatggtcaa ggtacgcgta
    12961 atgttgaagt tccagtcaaa gtttatccag ttgctaatgc aaaggcgcca tcacgtgatg
    13021 tgaaaggtca aaatttgact aatggaacgg atgcgatgaa ctacattaca tttgatccaa
    13081 atacaaacac aaatggtatc actgcagcat gggcaaatag acaacaacca aataaccaac
    13141 aagcaggcgt gcaacattta aatgtcgatg tcacatatcc aggtatttca gctgctaaac
    13201 gagttcctgt tactgttaat gtatatcaat ttgaattccc tcaaactact tatacgacaa
    13261 cggttggagg cactttagca agtggtacgc aagcatcagg atatgcacat atgcaaaatg
    13321 ctactggttt accaacagat ggatttacgt ataaatggaa tcgtgatact acaggtacaa
    13381 atgacgcaaa ctggtcagct atgaataaac cgaatgtggc taaagtcgtt aacgcaaaat
    13441 atgacgtcat ctataacgga catacttttg caacatcttt accagcgaaa tttgtagtaa
    13501 aagatgtgca accagcgaaa ccaactgtga ctgaaacagc ggcaggagcg attacaattg
    13561 cacctggagc aaaccaaaca gtgaatacac atgccggtaa cgtaacgaca tacgctgata
    13621 aattagttat taaacgtaat ggtaacgttg tgacgacatt tacacgtcgc aataatacga
    13681 gtccatgggt gaaagaagca tctgcagcaa ctgtagcagg tattgctgga actaataatg
    13741 gtattactgt tgcagcaggt actttcaacc ctgctgatac aattcaagtt gttgcaacgc
    13801 aaggaagcgg agagacagtg agtgatgagc aacgtagtga tgatttcaca gttgtcgcac
    13861 cacaaccgaa ccaagcgact actaagattt ggcaaaatgg tcatattgat atcacgccta
    13921 ataatccatc aggacattta attaatccaa ctcaagcaat ggatattgct tacactgaaa
    13981 aagtgggtaa tggtgcagaa catagtaaga caattaatgt tgttcgtggt caaaataatc
    14041 aatggacaat tgcgaataag cctgactatg taacgttaga tgcacaaact ggtaaagtga
    14101 cgttcaatgc caatactata aaaccaaatt catcaatcac aattactccg aaagcaggta
    14161 caggtcactc agtaagtagt aatccaagta cattaactgc accggcagct catactgtca
    14221 acacaactga aattgtgaaa gattatggtt caaatgtaac agcagctgaa attaacaatg
    14281 cagttcaagt tgctaataaa cgtactgcaa cgattaaaaa tggcacagca atgcctacta
    14341 atttagctgg tggtagcaca acgacgattc ctgtgacagt aacttacaat gatggtagta
    14401 ctgaagaagt acaagagtcc attttcacaa aagcggataa acgtgagtta atcacagcta
    14461 aaaatcattt agatgatcca gtaagcactg aaggtaaaaa gccaggtaca attacgcagt
    14521 acaataatgc aatgcataat gcgcaacaac aaatcaatac tgcgaaaaca gaagcacaac
    14581 aagtgattaa taatgagcgt gcaacaccac aacaagtttc tgacgcacta actaaagttc
    14641 gtgcagcaca aactaagatt gatcaagcta aagcattact tcaaaataaa gaagataata
    14701 gccaattagt aacgtctaaa aataacttac aaagttctgt gaaccaagta ccatcaactg
    14761 ctggtatgac gcaacaaagt attgataact ataatgcgaa gaagcgtgaa gcagaaactg
    14821 aaataactgc agctcaacgt gttattgaca atggcgatgc aactgcacaa caaatttcag
    14881 atgaaaaaca tcgtgtcgat aacgcattaa cagcattaaa ccaagcgaaa catgatttaa
    14941 ctgcagatac acatgcctta gagcaagcag tgcaacaatt gaatcgcaca ggtacaacga
    15001 ctggtaagaa gccggcaagt attactgctt acaataattc gattcgtgca cttcaaagtg
    15061 acttaacaag tgctaaaaat agcgctaatg ctattattca aaagccaata agaacagtac
    15121 aagaagtgca atctgcgtta acaaatgtaa atcgtgtcaa tgagcgatta acgcaagcaa
    15181 ttaatcaatt agtaccttta gctgataata gtgctttaaa aactgctaag acgaaacttg
    15241 atgaagaaat caataaatca gtaactactg atggtatgac acaatcatca atccaagcat
    15301 atgaaaatgc taaacgtgcg ggtcaaacag aatcaacaaa tgcacaaaat gttattaaca
    15361 atggtgatgc gactgaccaa caaattgccg cagaaaaaac aaaagtagaa gaaaaatata
    15421 atagcttaaa acaagcaatt gctggattaa ctccagactt ggcaccatta caaactgcaa
    15481 aaactcagtt gcaaaatgat attgatcagc caacgagtac gactggtatg acaagcgcat
    15541 ctattgcagc atttaatgaa aaactttcag cagctagaac taaaattcaa gaaattgatc
    15601 gtgtattagc ctcacatcca gatgttgcga caatacgtca aaacgtgaca gcagcgaatg
    15661 ccgctaaatc agcacttgat caagcacgta atggcttaac agtcgataaa gcgcctttag
    15721 aaaatgcgaa aaatcaacta caacatagta ttgacacgca aacaagtaca actggtatga
    15781 cacaagactc tataaatgca tacaatgcga agttaacagc tgcacgtaat aagattcaac
    15841 aaatcaatca agtattagca ggttcaccga ctgtagaaca aattaataca aatacgtcta
    15901 cagcaaatca agctaaatct gatttagatc atgcacgtca agctttaaca ccagataaag
    15961 cgccgcttca aactgcgaaa acgcaattag aacaaagcat taatcaacca acggatacaa
    16021 caggtatgac gaccgcttcg ttaaatgcgt acaaccaaaa attacaagca gcgcgtcaaa
    16081 agttaactga aattaatcaa gtgttgaatg gcaacccaac tgtccaaaat atcaatgata
    16141 aagtgacaga ggcaaaccaa gctaaggatc aattaaatac agcacgtcaa ggtttaacat
    16201 tagatagaca gccagcgtta acaacattac atggtgcatc taacttaaac caagcacaac
    16261 aaaataattt cacgcaacaa attaatgctg ctcaaaatca tgctgcgctt gaaacaatta
    16321 agtctaacat tacggcttta aatactgcga tgacgaaatt aaaagacagt gttgcggata
    16381 ataatacaat taaatcagat caaaattaca ctgacgcaac accagctaat aaacaagcgt
    16441 atgataatgc agttaatgcg gctaaaggtg tcattggaga aacgactaat ccaacgatgg
    16501 atgttaacac agtgaaccaa aaagcagcat ctgttaaatc gacgaaagat gctttagatg
    16561 gtcaacaaaa cttacaacgt gcgaaaacag aagcaacaaa tgcgattacg catgcaagtg
    16621 atttaaacca agcacaaaag aatgcattaa cacaacaagt gaatagtgca caaaacgtgc
    16681 aagcagtaaa tgatattaaa caaacgactc aaagcttaaa tactgctatg acaggtttaa
    16741 aacgtggcgt tgctaatcat aaccaagtcg tacaaagtga taattatgtc aacgcagata
    16801 ctaataagaa aaatgattac aacaatgcat acaaccatgc gaatgacatt attaatggta
    16861 atgcacaaca tccagttata acaccaagtg atgttaacaa tgctttatca aatgtcacaa
    16921 gtaaagaaca tgcattgaat ggtgaagcta agttaaatgc tgcgaaacaa gaagcgaata
    16981 ctgcattagg tcatttaaac aatttaaata atgcacaacg tcaaaactta caatcgcaaa
    17041 ttaatggtgc gcatcaaatt gatgcagtta atacaattaa gcaaaatgca acaaacttga
    17101 atagtgcaat gggtaactta agacaagctg ttgcagataa agatcaagtg aaacgtacag
    17161 aagattatgc ggatgcagat acagctaaac aaaatgcata taacagtgca gtttcaagtg
    17221 ccgaaacaat cattaatcaa acaacaaatc caacgatgtc tgttgatgat gttaatcgtg
    17281 caacttcagc tgttacttct aataaaaatg cattaaatgg ttatgaaaaa ttagcacaat
    17341 ctaaaacaga tgctgcaaga gcaattgatg cattaccaca tttaaataat gcacaaaaag
    17401 cagatgttaa atctaaaatt aatgctgcat caaatattgc tggcgtaaat actgttaaac
    17461 aacaaggtac agatttaaat acagcgatgg gtaacttgca aggtgcaatc aatgatgaac
    17521 aaacgacgct taatagtcaa aactatcaag atgcgacacc tagtaagaaa acagcataca
    17581 caaatgcggt acaagctgcg aaagatattt taaataaatc aaatggtcaa aataaaacga
    17641 aagatcaagt tactgaagcg atgaatcaag tgaattctgc taaaaataac ttagatggta
    17701 cgcgtttatt agatcaagcg aagcaaacag caaaacagca gttaaataat atgacgcatt
    17761 taacaactgc acaaaaaacg aatttaacaa accaaattaa tagtggtact actgtcgctg
    17821 gtgttcaaac ggttcaatca aatgccaata cattagatca agccatgaat acgttaagac
    17881 aaagtattgc caacaaagat gcgactaaag caagtgaaga ttacgtagat gctaataatg
    17941 ataagcaaac agcatataac aacgcagtag ctgctgctga aacgattatt aatgctaata
    18001 gtaatccaga aatgaatcca agtacgatta cacaaaaagc agagcaagtg aatagttcta
    18061 aaacggcact taacggtgat gaaaacttag ctgctgcaaa acaaaatgcg aaaacgtact
    18121 taaacacatt gacaagtatt acagatgctc aaaagaacaa tttgattagt caaattacta
    18181 gtgcgacaag agtgagtggt gttgatactg taaaacaaaa tgcgcaacat ctagaccaag
    18241 ctatggctag cttacagaat ggtattaaca acgaatctca agtgaaatca tctgagaaat
    18301 atcgtgatgc tgatacaaat aaacaacaag agtatgataa tgctattact gcagcgaaag
    18361 cgattttaaa taaatcgaca ggtccaaaca ctgcgcaaaa tgcagttgaa gcagcattac
    18421 aacgtgttaa taatgcgaaa gatgcattga atggtgatgc aaaattaatt gcagctcaaa
    18481 acgcagcgaa acaacattta ggtactttaa cgcatatcac tacagctcaa cgtaatgatt
    18541 taacaaatca aatttcacaa gctacaaact tagctggtgt tgaatctgtt aaacaaaatg
    18601 cgaatagttt agatggtgct atgggtaact tacaaacggc tatcaacgat aagtcaggaa
    18661 cattagcgag ccaaaacttc ttggatgctg atgagcaaaa acgtaatgca tacaatcaag
    18721 ctgtatcagc agccgaaacc attttaaata aacaaactgg accgaataca gcgaaaacag
    18781 cagtcgaaca agcacttaat aatgttaata atgcgaaaca tgcattaaat ggtacgcaaa
    18841 acttaaacaa tgcgaaacaa gcagcgatta cagcaatcaa tggcgcatct gatttaaatc
    18901 aaaaacaaaa agatgcatta aaagcacaag ctaatggtgc tcaacgcgta tctaatgcac
    18961 aagatgtaca gcacaatgcg actgaactga acacggcaat gggcacatta aaacatgcca
    19021 tcgcagataa gacgaatacg ttagcaagca gtaaatatgt taatgccgat agcactaaac
    19081 aaaatgctta cacaactaaa gttaccaatg ctgaacatat tattagcggt acgccaacgg
    19141 ttgttacgac accttcagaa gtaacagctg cagctaatca agtaaacagc gcgaaacaag
    19201 aattaaatgg tgacgaaaga ttacgtgaag caaaacaaaa cgccaatact gctattgatg
    19261 cattaacaca attaaataca cctcaaaaag ctaaattaaa agaacaagtg ggacaagcca
    19321 atagattaga agacgtacaa actgttcaaa caaatggaca agcattgaac aatgcaatga
    19381 aaggcttaag agatagtatt gctaacgaaa caacagtcaa aacaagtcaa aactatacag
    19441 acgcaagtcc gaataaccaa tcaacatata atagcgctgt gtcaaatgcg aaaggtatca
    19501 ttaatcaaac taacaatccg actatggata ctagtgcgat tacccaagct acaacacaag
    19561 tgaataatgc taaaaatggt ttaaacggtg ctgaaaactt aagaaatgca caaaacactg
    19621 ctaagcaaaa cttaaataca ttatcacact taacaaataa ccaaaaatct gccatctcat
    19681 cacaaattga tcgtgcaggt catgtgagtg aggtaactgc tactaaaaat gcagcaactg
    19741 agttgaatac gcaaatgggt aacttggaac aagctatcca tgatcaaaac acagttaaac
    19801 aaagtgttaa atttactgat gcagataaag ctaaacgtga tgcgtataca aatgcggtaa
    19861 gcagagctga agcaattctg aataaaacgc aaggtgcaaa tacgtctaaa caagatgttg
    19921 aagcggctat tcaaaatgtt tcaagtgcta aaaatgcatt gaatggtgat caaaacgtta
    19981 caaatgcgaa gaatgcagct aaaaatgcat taaataactt aacgtcaatt aataatgcac
    20041 aaaaacgtga cttaacaact aaaattgatc aagcaacaac tgtagctggt gttgaagctg
    20101 tatctaatac gagtacacaa ttgaatacag cgatggctaa cttgcaaaat ggtattaatg
    20161 ataaaacaaa tacactagca agtgaaaact atcatgatgc tgattcagat aagaaaactg
    20221 cttatactca agccgttacg aacgcagaaa atattttaaa taaaaatagt ggatcaaatt
    20281 tagacaaaac tgccgttgaa aacgcgttgt cacaagttgc taatgcgaaa ggtgccctaa
    20341 atggtaacca taatttagag caagctaaat caaatgcaaa cactactata aacggacttc
    20401 aacatttaac aactgctcaa aaagataaat tgaaacaaca agtgcaacaa gcacaaaatg
    20461 ttgcaggtgt agatactgtt aaatcaagtg ccaacacatt aaatggtgct atgggtacgt
    20521 taagaaatag catacaagat aacacagcta cgaaaaatgg ccaaaactat cttgatgcta
    20581 cagaacgtaa caaaacaaac tataacaatg ctgttgatag tgctaatggt gtcattaatg
    20641 caacaagcaa tccaaatatg gatgctaatg caattaacca aatcgctaca caagtgacat
    20701 caacgaaaaa tgcattagat ggtacacata atttaacgca agcgaaacaa acagcaacaa
    20761 atgccatcga tggtgctact aacttaaata aagcgcaaaa agatgcgtta aaagcacaag
    20821 ttacaagtgc gcaacgtgtt gcaaatgtaa caagtatcca acaaactgca aatgaactta
    20881 atacagctat gggtcaatta caacatggta ttgatgatga aaatgcaaca aaacaaactc
    20941 aaaaatatcg tgacgctgaa caaagtaaga aaactgctta tgatcaagct gtagctgctg
    21001 cgaaagcaat tttaaataaa caaacaggtt caaattcaga taaagcagca gttgaccgtg
    21061 cattacaaca agtaacaagt acgaaagatg cattgaatgg tgatgcaaaa ctggcagaag
    21121 cgaaagcggc agctaaacaa aacttaggca ctttaaacca tattacgaat gcacaacgta
    21181 ctgacttaga aggccaaatc aatcaagcga cgactgttga tggcgttaat actgtaaaaa
    21241 caaatgccaa tacattagac ggcgcaatga atagcttaca aggttcaatc aatgataaag
    21301 atgcgacatt aagaaatcaa aattatcttg atgcggatga atcaaaacga aatgcatata
    21361 cgcaagctgt cacagcggct gaaggcattt taaataaaca aactggtggt aacacatcta
    21421 aagcagacgt tgataatgca ttaaatgcag ttacaagagc gaaagcggct ttaaatggtg
    21481 ctgacaactt aagaaatgcg aaaacttcag caacaaatac gattgatggt ttacctaact
    21541 taacacaatt acaaaaagac aacttgaagc atcaagttga acaagcgcaa aatgtagcag
    21601 gtgtaaatgg tgttaaagat aaaggtaata cgttaaatac tgccatgggt gcattacgta
    21661 caagtatcca aaatgataat acgacgaaaa caagtcaaaa ttatcttgat gcatctgaca
    21721 gcaacaaaaa taattacaat actgctgtaa ataatgcaaa tggtgttatt aatgcaacga
    21781 acaatccaaa tatggatgct aatgcgatta atggcatggc aaatcaagtc aatacaacaa
    21841 aagcagcgtt aaatggtgca caaaacttag ctcaagctaa aacaaatgcg acgaacacaa
    21901 ttaacaacgc acatgactta aaccaaaaac aaaaagatgc attaaaaaca caagttaaca
    21961 atgcacaacg tgtatctgat gcaaataacg ttcaacacac tgcaactgaa ttgaacagtg
    22021 cgatgacagc acttaaagca gctattgctg ataaagaaag aacaaaagca agcggtaatt
    22081 atgtcaatgc tgatcaagaa aaacgtcaag cgtatgattc aaaagtgact aacgctgaaa
    22141 atatcattag tggtacaccg aatgcgacat taacagtcaa tgacgtaaat agtgcggcat
    22201 cacaagtcaa tgcggctaaa acagcattaa atggtgataa caacttacgt gtagcgaaag
    22261 agcatgccaa caatacaatt gacggcttag cacaattgaa taatgcacaa aaagcaaaat
    22321 taaaagaaca agttcaaagt gcaactacat tagatggtgt tcaaactgtt aaaaatagtt
    22381 ctcaaacgtt gaatacagcg atgaaaggct taagagatag tattgcgaat gaagcaacaa
    22441 ttaaagcagg tcaaaactac actgacgcaa gtccaaataa tcgtaacgag tacgacagtg
    22501 cagttactgc agcaaaagca atcattaatc aaacatcgaa cccaacgatg gaaccaaata
    22561 ctattacgca agtaacatca caagtgacaa ctaaagaaca ggcattaaat ggtgcgcgaa
    22621 acttagctca agctaagaca actgcgaaaa acaacttgaa taacttaaca tcaattaaca
    22681 atgcacaaaa agatgcgtta acgcgtagca ttgatggtgc aacaacagta gctggtgtaa
    22741 atcaagaaac tgcaaaagca acagaattaa ataacgcaat gcatagttta caaaatggta
    22801 tcaatgatga gacacaaaca aaacaaactc agaaatacct agatgcagag ccaagtaaga
    22861 aatcagctta tgatcaagca gtaaatgcag cgaaagcaat tttaacaaaa gctagtggtc
    22921 aaaatgtaga caaagcagca gttgaacaag cattgcaaaa tgtgaacagt acgaagacgg
    22981 cgttgaacgg tgatgcgaaa ttaaatgaag ctaaagcagc tgcgaaacaa acgttaggta
    23041 cattaacaca cattaataat gcacaacgta cagcgttaga caatgaaatt acacaagcaa
    23101 caaatgttga aggtgttaat acagttaaag ccaaagcgca acaattagat ggtgctatgg
    23161 gtcaattaga aacatcaatt cgtgataaag acacgacgtt acaaagtcaa aattatcaag
    23221 atgctgatga tgctaaacga actgcttatt ctcaagcagt aaatgcagca gcaactattt
    23281 taaataaaac agctggcggt aatacaccta aagcagatgt tgaaagagca atgcaagctg
    23341 ttacacaagc aaatactgca ttaaacggta ttcaaaactt agatcgtgcg aaacaggctg
    23401 ctaacacagc gattacaaat gcttcggact taaatacaaa acaaaaagaa gcattaaaag
    23461 cacaagtaac aagtgcagga cgtgtatctg cagcaaatgg tgttgaacat actgcgactg
    23521 aattaaatac tgcgatgaca gctttaaagc gtgccattgc tgataaagct gagacaaaag
    23581 ctagtggtaa ctatgtcaat gctgatgcga ataaacgtca agcatatgat gaaaaagtta
    23641 cagctgccga aaatatcgtt agtggtacac caacaccaac gttaacacca gcagatgtta
    23701 caaatgcagc aacgcaagta acgaatgcta agacgcagtt aaacggtaat cataatttag
    23761 aagtagcgaa acaaaatgct aacactgcaa ttgatggttt aacttcttta aatggtccgc
    23821 aaaaagcaaa acttaaagaa caagtgggtc aagcgacgac gttgccaaat gttcaaactg
    23881 ttcgtgataa tgcacaaaca ttaaacactg caatgaaagg tctacgagat agcattgcga
    23941 atgaagcaac gattaaagca ggtcaaaact acacagatgc aagtcaaaac aaacaaactg
    24001 actacaacag tgcagtcact gcagcaaaag caatcattgg tcaaacaact agtccatcaa
    24061 tgaatgcgca agaaattaat caagcgaaag accaagtgac agctaaacaa caagcgttaa
    24121 acggtcaaga aaacttaaga actgcgcaaa caaatgcgaa gcaacatttg aacggcttaa
    24181 gtgacttaac tgacgctcaa aaagatgcag tgaaacgtca aatcgaaggt gcaacgcatg
    24241 ttaatgaagt aacacaagca caaaataatg cggatgcatt aaatacagct atgacgaact
    24301 tgaaaaatgg tattcaagat cagaatacga ttaagcaagg tgttaacttc actgatgccg
    24361 acgaagcgaa acgtaatgca tatacaaatg cagtgacgca agctgaacaa attttaaata
    24421 aagcacaagg tccaaatact tcaaaagacg gtgtcgaaac tgcgttagaa aatgtacaac
    24481 gtgctaaaaa cgaattgaac ggtaatcaaa atgttgcgaa cgctaagaca actgcgaaaa
    24541 atgcattgaa taacctaaca tcaattaata atgcacaaaa agaagcattg aaatcacaaa
    24601 ttgaaggtgc gacaacagtt gcaggtgtaa atcaagtgtc tacaacggca tctgaattaa
    24661 atacagcaat gagcaactta caaaatggta ttaatgatga agcagctaca aaagcagctc
    24721 agaaatatac tgatgcagat agagaaaaac aaactgcata caatgacgct gtaacagcag
    24781 ctaaaacgtt attagataaa acagctggtt caaatgacaa taaagcagct gttgaacaag
    24841 cattacaacg tgtgaatact gctaaaacag cattaaatgg tgacgagcga ttaaatgaag
    24901 cgaagaacac agctaaacaa caagtagcga caatgtcaca cttaactgat gctcaaaaag
    24961 caaacttaac atcgcaaatc gaaagtggta cgactgttgc aggtgttcaa ggtattcaag
    25021 ctaatgccgg tactttagat caagcaatga atcaattaag acaaagtatt gcttctaaag
    25081 atgcgactaa atcaagcgaa gattatcaag acgcgaatgc agatttacaa aatgcataca
    25141 atgatgcggt aactaatgct gaaggtatta ttagtgcaac gaataaccct gaaatgaatc
    25201 ctgatacaat taaccaaaaa gcgagccaag tgaacagtgc gaagtctgca ttgaacggtg
    25261 atgaaaaatt agcagcagca aaacaaactg cgaaatcaga tatcggtcgt ttgacagact
    25321 tgaacaatgc acaacgaact gcggcaaatg ctgaagtgga tcaagcacca aatcttgcag
    25381 ctgtcacagc ggctaaaaat aaagcaacat cgttaaacac agcgatgggt aatttgaaac
    25441 atgcacttgc tgaaaaggat aatacgaaac gtagtgtcaa ttacacagat gcggatcaac
    25501 caaaacaaca agcgtatgat actgcggtta cacaagcaga agcaattact aatgctaatg
    25561 gcagcaacgc gaatgaaaca caagttcaag cagcactaaa ccaattgaat caagctaaaa
    25621 atgacttgaa tggtgataat aaagttgctc aagcaaaaga gtcagcgaaa cgtgcattag
    25681 cttcatatag taacttgaat aatgcgcaat caactgcagc aattagtcaa attgacaatg
    25741 caacgacagt agcaggcgta actgctgcac aaaatactgc taatgaatta aatacagcaa
    25801 tgggtcaact tcaaaatggt ataaatgacc aaaacactgt taaacaacaa gtgaacttta
    25861 cagatgctga ccaaggtaag aaagatgctt acacaaatgc tgttacgaat gctcaaggta
    25921 ttttagataa agcacacggt caaaatatga cgaaagcaca agttgaagct gcattaaatc
    25981 aagtaacgac tgctaagaat gctttaaacg gtgacgcaaa tgtaagacaa gcaaaatcag
    26041 atgcgaaagc aaacttaggt acattaacac acttaaataa tgcacaaaaa caagatttaa
    26101 catcacaaat cgaaggcgca acaacagtca acggtgtaaa tggtgttaaa acgaaagcac
    26161 aagacttaga tggtgcaatg caacgattac aatcagctat agcaaataaa gatcaaacta
    26221 aagcgagcga aaattacatc gacgcagatc caactaagaa aacagcattt gataatgcta
    26281 tcacacaagc tgaatcttac ttaaataaag atcacggtgc gaataaagat aagcaagctg
    26341 ttgaacaagc aattcaaagt gtaacgtcta ctgaaaatgc tttgaacggt gacgcgaact
    26401 tacaacgcgc taaaactgaa gctatacaag ctatcgataa cttgacacat ttgaatacac
    26461 cacaaaaaac agcattaaaa caacaagtga acgctgcgca acgtgtatca ggtgtaactg
    26521 atctgaaaaa tagtgctaca tcacttaata atgcgatgga tcaattaaaa caagcaattg
    26581 ctgatcatga cacaattgta gctagtggta attacactaa cgcgagtcct gataagcaag
    26641 gtgcttatac tgatgcatat aatgctgcga aaaacattgt aaatggttcg cctaatgtga
    26701 ttacaaatgc agcagatgtt acagcagcaa cacaacgtgt taataatgct gaaacaggtt
    26761 taaacggtga tacaaactta gcaactgcga agcaacaagc taaagatgca ttacgtcaaa
    26821 tgacacattt atctgatgca caaaaacaaa gtattactgg tcaaattgat agcgcgacac
    26881 aagtaactgg cgttcaaagt gtgaaagaca acgcgacaaa tcttgataat gcaatgaatc
    26941 aacttcgaaa tagtattgcg aataaagatg atgtaaaagc gagtcaacca tatgttgatg
    27001 cagatagaga taaacaaaat gcatacaata cagcagttac aaatgctgaa aatatcatta
    27061 atgcaacgag tcagccgaca cttgatccat ctgcagtaac acaagcagct aatcaagtga
    27121 gcactaacaa aactgcgctt aatggtgcac aaaacttagc gaataaaaag caagaaacga
    27181 ctgctaacat caaccaatta agtcatttaa ataatgctca aaagcaagat ttaaatacgc
    27241 aagtgacaaa tgcaccaaat attagcacag taaatcaagt gaaaactaaa gctgaacaat
    27301 tagatcaagc aatggaacgt ttaatcaacg gaatccaaga caaagatcaa gtgaaacaaa
    27361 gtgttaactt tacagatgca gatccagaaa aacaaacagc atacaacaat gcggtaactg
    27421 ctgctgaaaa tattattaat caagcaaatg gtacaaatgc gaaccaatca caagttgaag
    27481 cagcactttc aactgtaaca actacgaaac aagcgttgaa tggtgataga aaagtaacag
    27541 atgctaaaaa caatgcaaac caaacattat ctacgttaga taacttaaac aatgcacaaa
    27601 aaggtgctgt tactggaaac atcaatcaag cgcacactgt agctgaagta acgcaagcca
    27661 ttcaaaccgc tcaggaactg aatacagcga tgggtaactt gaaaaatagc ttgaatgata
    27721 aagacactac acttggcagt caaaactttg cagatgcaga tccagagaag aaaaatgcat
    27781 acaatgaagc ggttcataat gctgaaaata ttttaaataa atctacaggt acgaacgtgc
    27841 ctaaagatca agttgaagca gctatgaatc aagtgaatgc tacaaaagca gcgcttaatg
    27901 gtactcaaaa ccttgaaaaa gctaaacaac acgcaaatac agcaattgac ggtttaagcc
    27961 atttaacaaa tgcacaaaaa gaggcattaa aacaattggt acaacaatcg actactgttg
    28021 cagaagcaca aggtaatgag caaaaagcaa acaatgttga tgcagcaatg gacaaattac
    28081 gtcaaagtat tgcagataat gcgacaacaa aacaaaacca aaattatact gatgcaagtc
    28141 agaataaaaa ggatgcgtac aataatgctg tcacaactgc acaaggtatt attgatcaaa
    28201 ctacaagtcc aactttagat ccgactgtta tcaatcaagc tgctggacaa gtaagcacaa
    28261 ctaaaaatgc attaaatggt aatgaaaacc tagaggcagc gaaacaacaa gcgtcacaat
    28321 cattaggttc attagataac ttaaataatg cgcaaaaaca aacagttact gatcaaatta
    28381 atggcgcgca tactgttgat gaagcaaatc aaattaagca aaatgcgcaa aacttaaata
    28441 cagcgatggg taacttgaaa caagcgatag ctgacaaaga tgctacgaaa gcgacagtta
    28501 acttcactga tgcagatcaa gcaaaacaac aagcatataa cactgctgtt acaaatgctg
    28561 aaaatatcat ttcaaaagct aatggcggca atgcaacaca agctgaagtt gaacaagcaa
    28621 tcaaacaagt taatgctgca aaacaagcat taaatggtaa tgccaacgtt caacatgcaa
    28681 aagacgaagc aacagcatta attaatagct ctaatgacct taaccaagca caaaaagacg
    28741 cattaaaaca acaagttcaa aatgcaacta ctgtagctgg tgtaaacaat gttaaacaaa
    28801 cagcacaaga gttaaacaat gctatgacac aattaaaaca aggcattgca gataaagaac
    28861 aaacaaaagc tgatggtaac tttgtcaatg cagatcctga taagcaaaat gcatataatc
    28921 aagcagtagc gaaagctgaa gcattaatta gtgctacgcc tgatgttgtc gttacaccta
    28981 gcgaaattac tgcagcgtta aataaagtta cgcaagctaa aaatgattta aatggtaata
    29041 caaacttagc aacggcgaaa caaaatgttc aacatgctat tgatcaattg ccaaacttaa
    29101 accaagcgca acgtgatgaa tacagcaaac aaatcacgca agcaacactt gtaccaaacg
    29161 tcaatgctat tcaacaagcg gcgacaacgc ttaatgacgc gatgacacaa ttgaaacaag
    29221 gtattgcgaa taaagcacaa attaaaggta gcgagaacta tcacgatgct gatactgaca
    29281 agcaaacagc atatgataat gcagtaacaa aagcagaaga attgttaaaa caaacaacaa
    29341 atccaacaat ggatccaaat acaattcaac aagcattaac taaagtgaat gacacaaatc
    29401 aagcacttaa cggtaatcaa aaattagctg atgccaaaca agatgctaag acaacacttg
    29461 gtacactaga tcatttaaat gatgctcaaa aacaagcgct aacaactcaa gttgaacaag
    29521 caccagatat tgcaacagtt aataatgtta agcaaaatgc tcaaaatctg aataatgcta
    29581 tgactaactt aaacaatgca ttacaagata aaactgagac attaaatagc attaacttta
    29641 ctgatgcaga tcaagctaag aaagatgctt atactaatgc ggtttcacat gcagaaggta
    29701 ttttatctaa agcaaatggc agcaatgcaa gtcaaactga agtggaacaa gcgatgcaac
    29761 gtgtgaacga agcgaaacaa gcattgaatg gtaatgacaa tgtacaacgt gcaaaagatg
    29821 cagcgaaaca agtgattaca aatgcaaatg atttaaatca agcgcaaaaa gatgcattaa
    29881 aacaacaagt cgatgctgcg caaactgttg caaatgtaaa cacgattaag caaacagcac
    29941 aagatttaaa tcaagcaatg acacaattga aacaaggtat tgcagataaa gaccaaacta
    30001 aagcaaatgg taactttgtc aatgctgata ctgataagca aaatgcttac aacaatgcgg
    30061 tagcacatgc tgaacaaata attagtggta caccaaatgc aaacgtggat ccacaacaag
    30121 tggctcaagc gttacaacaa gtgaatcaag ctaagggtga tttaaacggt aaccataact
    30181 tacaagttgc taaagacaat gcaaatacag ccattgatca gttaccaaac ttaaatcaac
    30241 cacaaaaaac agcattaaaa gaccaagtgt cgcatgcaga acttgttaca ggtgttaatg
    30301 ctattaagca aaatgctgat gcgttaaata atgcaatggg tacattgaaa caacaaattc
    30361 aagcgaacag tcaagtacca cagtcagttg actttacaca agcggatcaa gacaaacaac
    30421 aagcatataa caatgcggct aaccaagcgc aacaaatcgc aaatggcata ccaacacctg
    30481 tattgacgcc tgatacagta acacaagcag tgacaactat gaatcaagcg aaagatgcat
    30541 taaacggtga tgaaaaatta gcacaagcga aacaagaagc tttagcaaat cttgatacgt
    30601 tacgcgattt aaatcaacca caacgtgatg cattacgtaa ccaaatcaat caagcacaag
    30661 cgttagctac agttgaacaa actaaacaaa atgcacaaaa tgtgaataca gcaatgagta
    30721 acttgaaaca aggtattgca aacaaagata ctgtcaaagc aagtgagaac tatcatgatg
    30781 ctgatgccga taagcaaaca gcatatacaa atgcagtgtc tcaagcggaa ggtattatca
    30841 atcaaacgac aaatccaacg cttaacccag atgaaataac acgtgcatta actcaagtga
    30901 ctgatgctaa aaatggctta aacggtgaag ctaaattggc aactgaaaag caaaatgcta
    30961 aagatgccgt aagtgggatg acgcatttaa acgatgctca aaaacaagca ttaaaaggtc
    31021 aaatcgatca atcgcctgaa attgctacag tgaaccaagt taaacaaaca gcaacgagcc
    31081 tagatcaagc aatggatcaa ttatcacaag ctattaatga taaagctcaa acattagcgg
    31141 acggtaatta cttaaatgca gatcctgaca aacaaaatgc gtataaacag gcagtagcaa
    31201 aagctgaagc attattgaat aaacaaagtg gtactaatga agtacaagca caagttgaaa
    31261 gcatcactaa tgaagtgaac gcagcgaaac aagcattaaa tggtaatgac aatttggcaa
    31321 atgcaaaaca acaagcaaaa caacaattgg cgaacttaac acacttaaat gatgcacaaa
    31381 aacaatcatt tgaaagtcaa attacacaag cgccacttgt tacagatgtc actacgatta
    31441 atcaaaaagc acaaacgtta gatcatgcga tggaattatt aagaaatagt gttgcggata
    31501 atcaaacgac attagcgtct gaagattatc atgatgcaac tgcgcaaaga caaaatgact
    31561 ataaccaagc tgtaacagct gctaataata tcattaatca aactacatcg cctacgatga
    31621 atccagatga tgttaatggt gcaacgacac aagtgaataa tacgaaagtt gcattagatg
    31681 gtgatgaaaa ccttgcagca gctaaacaac aagcaaacaa cagacttgat caattagatc
    31741 atttgaataa tgcgcaaaag caacagttac aatcacaaat tacgcaatca tctgatattg
    31801 ctgcagttaa tggtcacaaa caaacagcag aatctttaaa tactgcgatg ggtaacttaa
    31861 ttaatgcgat tgcagatcat caagccgttg aacaacgtgg taacttcatc aatgctgata
    31921 ctgataaaca aactgcttat aatacagcgg taaatgaagc agcagcaatg attaacaaac
    31981 aaactggtca aaatgcgaac caaacagaag tagaacaagc tattactaaa gttcaaacaa
    32041 cacttcaagc gttaaatgga gaccataatt tacaagttgc taaaacaaat gcgacgcaag
    32101 caattgatgc tttaacaagc ttaaatgatc ctcaaaaaac agcattaaaa gaccaagtta
    32161 cagctgcaac tttagtaact gcagttcatc aaattgaaca aaatgcgaat acgcttaacc
    32221 aagcaatgca tggtttaaga cagagcattc aagataacgc agcaactaaa gcaaatagca
    32281 aatatatcaa cgaagatcaa ccagagcaac aaaactatga tcaagctgtt caagccgcaa
    32341 ataatattat caatgaacaa actgcaacat tagataataa tgcgattaat caagcagcga
    32401 caactgtgaa tacaacgaaa gcagcattac atggtgatgt gaagttacaa aatgataaag
    32461 atcatgctaa gcaaacggtt agtcaattag cacatctaaa caatgcacaa aaacatatgg
    32521 aagatacgtt aattgatagt gaaacaacta gaacagcagt taagcaagat ttgactgaag
    32581 cacaagcatt agatcaactt atggatgcat tacaacaaag tattgctgac aaagatgcaa
    32641 cacgtgcgag cagtgcatat gtcaatgcag aaccgaataa aaaacaatcc tatgatgaag
    32701 cagttcaaaa tgctgagtct atcattgcag gattaaataa tccaactatc aataaaggta
    32761 atgtatcaag tgcgactcaa gcagtaatat catctaaaaa tgcattagat ggtgttgaac
    32821 gattagctca agataagcaa actgctggaa attctctaaa tcatttagat caattaacac
    32881 cagctcaaca acaagcgcta gaaaatcaaa ttaataatgc aacaactcgt ggcgaagtag
    32941 cacaaaaatt aactgaagca caagcactta accaagcaat ggaagcttta cgtaatagca
    33001 ttcaagatca acagcaaacg gaagcgggta gcaagtttat caatgaagat aaaccacaaa
    33061 aagatgctta ccaagcagca gttcaaaatg caaaagattt aattaatcaa actaacaatc
    33121 caacgcttga taaagcacaa gttgaacaat tgacacaagc tgttaaccaa gctaaagata
    33181 acctacacgg tgatcaaaaa cttgcagacg ataaacaaca tgcggttact gatttaaatc
    33241 aattaaatgg tttgaataat ccgcaacgtc aagcacttga aagccaaata aacaacgcag
    33301 caactcgtgg cgaagtagca caaaaattag ctgaagcaaa agcgcttgat caagcaatgc
    33361 aagcattacg taatagtatt caagatcaac aacaaacaga atctggtagc aagtttatca
    33421 atgaagataa accgcaaaaa gatgcttacc aagcagcagt tcaaaatgca aaagatttaa
    33481 ttaaccaaac aggtaatcca acactcgaca aatcacaagt agaacaattg acacaagcag
    33541 taacaactgc aaaagataat ctacatggtg atcaaaaact tgctcgtgat caacaacaag
    33601 cagtaacaac tgtaaatgca ttgccaaact taaatcatgc acaacaacaa gcattaactg
    33661 atgctataaa tgcagcgcct acaagaacag aggttgcaca acatgttcaa actgctactg
    33721 aacttgatca cgcgatggaa acattgaaaa ataaagttga tcaagtgaat acagataagg
    33781 ctcaaccaaa ttacactgaa gcgtcaactg ataaaaaaga agcagtagat caagcgttac
    33841 aagctgcaga aagcattaca gatccaacta atggttcaaa tgcgaataaa gacgctgtag
    33901 accaagtatt aactaagctt caagaaaaag aaaatgagtt aaatggtaat gagagagtcg
    33961 ctgaagctaa aacacaagcg aaacaaacta ttgaccaatt aacacattta aatgctgatc
    34021 aaattgcaac tgctaaacaa aacattgatc aagcgacgaa acttcaacca attgctgaat
    34081 tagtagatca agcaacgcaa ttgaatcaat ctatggatca attacaacaa gcagttaatg
    34141 aacatgctaa cgttgagcaa actgtagatt acacacaagc agattcagat aaacaaaatg
    34201 cttataaaca agctattgct gatgctgaaa atgtattgaa acaaaatgcg aataagcaac
    34261 aagtggatca agcacttcaa aatattttaa atgcaaaaca agcattaaat ggtgatgaac
    34321 gtgtagcact tgctaaaaca aatggtaaac atgacatcga ccaattgaat gcattaaaca
    34381 atgctcaaca agatggattt aaaggtcgca tcgatcaatc aaacgattta aatcaaatcc
    34441 aacaaattgt agatgaggct aaggcactta atcgtgcaat ggatcaattg tcacaagaaa
    34501 tcactgacaa tgaaggacgc acgaaaggta gcacgaacta tgtcaatgca gatacacaag
    34561 tcaaacaagt atatgatgaa acggttgata aagcgaaaca agcacttgat aaatcgactg
    34621 gtcaaaactt aactgcaaaa caagttatca aattaaatga tgcagtcact gcagctaaga
    34681 aagcattaaa tggtgaagaa agacttaata atcgtaaagc tgaagcatta caaagattgg
    34741 atcaattaac acatctaaac aatgctcaaa gacaattagc aatccaacaa attaataatg
    34801 ctgaaacgct aaataaagca tctcgagcaa ttaatagagc aactaaatta gataatgcaa
    34861 tgggtgcagt acaacaatat attgacgaac agcaccttgg tgttatcagc agcacaaatt
    34921 acatcaatgc agatgacaat ttgaaagcaa attatgataa tgcaattgcg aatgcagcac
    34981 atgagttaga taaagtgcaa ggtaatgcaa ttgcaaaagc tgaagcagag caattgaaac
    35041 aaaatattat cgatgctcaa aatgcattaa atggagacca aaaccttgca aatgccaaag
    35101 ataaagcaaa tgcgtttgtt aattcgttaa atggattaaa tcaacagcaa caagatcttg
    35161 cacataaagc aattaacaat gccgatactg tatcagatgt aacagatatt gttaataatc
    35221 aaattgactt aaatgatgca atggaaacat tgaaacattt agttgacaat gaaattccaa
    35281 atgcagagca aactgtcaat taccaaaacg ctgacgataa tgctaaaaca aacttcgatg
    35341 atgccaaacg tctagcaaat acattgctaa atagtgataa cacaaatgtg aatgatatca
    35401 atggcgcaat ccaagcagtc aatgatgcaa tccataatct taatggtgat caacgactac
    35461 aagatgctaa agacaaggca attcaatcta ttaatcaagc tttagctaat aagctaaaag
    35521 aaatcgaagc ttcaaatgcg acggatcaag acaagcttat tgcgaaaaat aaagcagaag
    35581 aattggcaaa cagcatcatc aacaacatta ataaagcaac aagtaatcag gctgtatctc
    35641 aagttcaaac agcaggcaac cacgcgattg aacaagtgca tgccaatgaa ataccaaaag
    35701 caaaaattga tgccaataaa gacgttgata agcaagttca agcattaatt gacgaaattg
    35761 atcgaaatcc aaatctaaca gataaggaaa aacaagcact taaagatcgt attaatcaaa
    35821 tacttcaaca aggtcataac ggcattaaca atgcgatgac taaagaagaa attgaacaag
    35881 ccaaagcaca acttgcgcaa gcattacaag acatcaaaga tttagtgaaa gctaaagaag
    35941 atgcgaaaca agatgttgat aaacaagttc aagctttaat tgacgaaatc gatcaaaatc
    36001 caaatctaac agataaggaa aaacaagcac ttaaatatcg aattaatcaa atacttcaac
    36061 aaggtcataa cgacattaac aatgcgctga ctaaagaaga aattgagcag gcaaaagcac
    36121 aacttgcaca agcattgcaa gacatcaaag atttagtgaa agctaaagaa gatgcgaaaa
    36181 atgcaataaa agccttagct aatgcgaagc gtgatcaaat caattcaaat ccagatttaa
    36241 cacctgagca aaaagcaaaa gcgctcaaag aaattgacga agctgaaaaa cgagcactac
    36301 aaaacgttga gaatgctcaa actatagatc aattaaatcg aggattaaac ttaggtttag
    36361 atgacattag aaatacacat gtatgggagg ttgatgaaca acctgctgta aatgaaattt
    36421 ttgaagcaac acctgagcaa atcctagtta atggtgaact cattgtacat cgtgatgaca
    36481 tcattacaga acaagatatt cttgcacaca taaacttaat tgatcagctt tcagcagaag
    36541 tcatcgatac accatcaact gcaacgattt ctgatagctt aacagcaaaa gttgaagtta
    36601 cattgcttga tggatcaaaa gtgattgtta atgttcctgt aaaagttgta gaaaaagaat
    36661 tgtcagtagt caaacaacag gcaattgaat caatcgaaaa tgcggcacaa caaaagatta
    36721 atgaaatcaa taatagtgtg acattaacac tggaacaaaa agaagctgca attgcagaag
    36781 ttaataagct taaacaacaa gcaattgatc atgttaacaa tgcacctgat gttcattcag
    36841 ttgaagaaat tcaacaacaa gaacaagcgc atattgaaca atttaatcca gaacaattta
    36901 cgattgaaca agcaaaatca aatgcaatta aatcgattga agatgcaatt caacatatga
    36961 ttgatgaaat caaagctcgt actgatctaa cagataaaga gaagcaagaa gctattgcta
    37021 agttaaatca attaaaagaa caagcaattc aagcgattca acgtgcgcaa agcatcgatg
    37081 aaataagtga gcaattggaa caatttaaag ctcaaatgaa agcagctaat ccaacagcaa
    37141 aagaactagc taaacgcaag caagaagcta ttagtagaat taaagacttt tcaaatgaaa
    37201 aaataaatag tattcgaaat agtgaaattg gcacagctga tgaaaaacaa gcagcaatga
    37261 atcaaattaa cgaaattgtg cttgaaacaa ttagagatat taataatgcg catacattac
    37321 agcaagttga ggctgcattg aacaatggta ttgctcgaat ttcagcagta caaattgtaa
    37381 catctgatcg tgctaaacaa tcgtcaagta ctggaaatga atctaatagc catttaacaa
    37441 ttggttatgg aactgcaaat catccattta acagttcgac tattggacat aaaaagaaac
    37501 ttgatgaaga tgatgacatt gatccacttc atatgcgtca ctttagtaat aatttcggta
    37561 atgttattaa aaacgctatt ggtgtggtgg gtatctctgg tttactagct agtttctggt
    37621 tcttcattgc caaacgtcgt cgtaaagaag atgaagagga agaattagaa ataagagata
    37681 ataataaaga ttcaataaaa gagactttag acgatacaaa acatttacca cttttatttg
    37741 cgaaacgtcg cagaaaagaa gatgaagaag atgttactgt tgaagaaaaa gattcgctaa
    37801 ataatggcga gtcactcgat aaagttaaac atacgccgtt cttcttacca aaacgtcgtc
    37861 gtaaagaaga tgaagaagat gtggaagtta caaatgaaaa cacagatgaa aaagtgttga
    37921 aagataacga acattcacca ctcttattcg caaaacgacg caaagataaa gaggaagatg
    37981 ttgaaacaac aactagtatt gaatctaaag atgaggacgt tcctttatta ttggctaaaa
    38041 agaaaaatca aaaagataac caatccaaag acaaaaagtc agcatcaaaa aatacttcta
    38101 aaaaggtagc agctaaaaag aagaaaaaga aagctaagaa aaataaaaaa taatttgttt
    38161 ctttgataaa tagaggagca ccgattgaca tcacatcagt cggtgctcct tttatttatt
    38221 ctttttaatt aatttataca atgcctgttg agcgtgttga ttcgcttctt tgttttgttc
    38281 tctcggtatc catttaacaa ataataaatc aaaatctttt tcaaatattt ctatttgatc
    38341 aaaataaggt ttgaaatttg cgtttttcac ataaccagct tcaatgctat ctgcaattag
    38401 ctttgagtct gtatataata gtgcgttttg aacatttaat tcacgtgcat gttctagtgc
    38461 ataaatacat gcagcccatt ctgcagtgtg gttatccatt tcgcctaact catgtgtata
    38521 tgtataatgc tgctcatctt ctttgattac aatggcacat gtacttatgc ctggatttcc
    38581 tttcgtcgca gcatcaaaat ttattttcgc cataataaac ctactttcta ttcaatactt
    38641 agttaaagtt actattactg taatacaaaa tatgttgggt aatccattaa aaaacacgca
    38701 tcacttaaat aagtaacacg tgtttaaaat actcgctgat tcaaagatga ttttctaata
    38761 cgtagtactg taatatactt cctaaaaaaa tcatcttcag gctgggacat aaatcaatgt
    38821 tctatgctct acgatgttat attggcagta gttgactgaa cgaaaatgcg cttgtaacaa
    38881 gcttttttca attctagtca ggggccccaa cacagagaat ttcgaaaaga aattctacag
    38941 gcaatgcgag ttggggtgtg ggtcccaaca cagaagatga cgaaaagtca gcttacaata
    39001 atgtgcaagt ttgggatggg ccccaacaaa gagaaattgg attcccaatt tctacagaca
    39061 atgcaagttg gggtgggacg acgaaataaa ttttgcgaaa atattatttc tgtcccactc
    39121 ccttaaaact tattcttttg tgtagtaagt gcgttaatag ccttgatcta acttatcaat
    39181 cttaccttta cgataaaatg atttagcaat atatccaaat ggtacattga aaactgttga
    39241 agctaatttt aatacgtacg ttgtaataaa tatttcaaat acaactgtac caggtaaact
    39301 tccgataaat gcgatagcta caaataaagc tgtatcaatt attgagctta aaaatgtact
    39361 accatatgca cggatgaaaa acgttttatc agaactaaat acttttttaa ttagtgaaaa
    39421 gataaataca tcaatatgtt gaccaataat atatgcgacg attgagccta aagcaatgcg
    39481 tggcacaaca tcaaagattg cgtgtaatgc tttttgtgcc atatcttctg gtgcaggaat
    39541 aaaatgtaat gacaattgca taacaataat cataattaat gttgatgaaa agcctaacca
    39601 aactgctctt tttgcaactc tacgcccata aatatcgttt aatatatctg ttgctaaata
    39661 aatagaagca aacatgacat tacctaaagt tgctgaaata ccaaagattt ctacagtttt
    39721 aatcacttgt atgttggcaa tgattgtgcc aattgcaacc catgcaatta aaccttgttt
    39781 accaaaaaag cgatacataa gtaccataag cacgaacgtt gcaataaacg taactagtcc
    39841 taaaatttca ttatacatat taaaatgtcc tcctaaattt tgatcatgcg ggtgtttaga
    39901 aaccgctcaa taaataacta actcatttta ctgaaaaatc gtttgcgatg caatagcatt
    39961 tgaatataaa tacattttac gtatagaatt gtaaaaggtt tcattcaaat cttagggtca
    40021 aaaatgttat aatattttta tgtcaaattt aaaacagtaa cacttattta caaggttgca
    40081 atattttgaa gtaataaagg aagtgtcgcg tattttaact ttttcagagc aaaatgcact
    40141 cgcgaaaata gatgatttaa tgaatactta ttgcaatcaa tgtccaatca aaactcgtct
    40201 gcgtaaatta gaggggaaaa cgaaggcgca tcatttttgt atcaatgagt gttcaatagg
    40261 gaaagaaata aaacaattag gaaatgaact tcaataggag gaagtcaaat gaaaattata
    40321 tctatatcag aaacaccgaa ccacaacaca atgaagatta cacttagtga aagcagagaa
    40381 ggtatgacat cagatacgta tactaaagtt gatgattcac agccagcatt tattaatgac
    40441 atcttaaagg ttgaaggcgt taaatcaatt ttccatgtta tggactttat ttcagtagat
    40501 aaagaaaatg acgcaaattg ggaaacagta ttgccaaaag tagaggctgt attcgaataa
    40561 atttttcatc aactagtatt cggggggaat aaagtatatg gaaattttac gtatagagcc
    40621 aacaccaagt ccaaatacaa tgaaagttgt tttgtcatat acaagagaag acaagttatc
    40681 taatacttat aaaaaagtag aagaaacaca accaagattt ataaatcagt tgttatctat
    40741 agatggtatc acttccattt ttcatgtcat gaacttctta gctgttgata aggcaccaaa
    40801 agctgattgg gaagtcatat tacctgatat taaagctgct ttttctgatg cgaataaggt
    40861 tttagaatct gtaaatgaac ctcaaattga caatcatttt ggtgaaatta aagctgaatt
    40921 attaactttt aagggtatac cgtatcaaat taagctaact tctgctgacc aagaattaag
    40981 agaacaatta ccacaaacat atgttgacca tatgactcaa gcgcaaacag cacatgacaa
    41041 tattgttttt atgcgtaaat ggctagattt aggaaatcgc tatggaaata ttcaagaagt
    41101 aatggatggt gtcctagaag aagtgctaac tacctatcca gaatcacagt tacccgtatt
    41161 ggtaaaacat gctttagaag aaaatcacgc aactaataat tatcatttct atcgacatgt
    41221 ctctttggat gaatatcatg caactgataa ttggaagact cgattacgaa tgttaaacca
    41281 ttttccaaag ccgacttttg aagatatacc gctgcttgat ttagctttat ctgatgaaaa
    41341 agtaccggtt agacgtcaag cgattgtatt attaggtatg attgaaagta aagaaatttt
    41401 accgtattta tataaggggc ttcgtgataa aagtcctgtt gtaagaagaa cagcagggga
    41461 ttgcataagc gatttagggt atccagaggc actaccagaa atggtgctac tattagatga
    41521 tccacagaaa atcgttaggt ggcgtgctgc tatgtttatc tttgatgaag gtaatgcaga
    41581 gcagcttccc gcactaaaag cccatattaa tgacaatgcg tttgaagtta aattacaaat
    41641 tgaaatggcc atatctcgta ttgaaaacgg agatgaagca cttggttcag tatggaaaca
    41701 aatggcaaac agaacaattt aattggagga attaaatatg aatgcatatg atgcttatat
    41761 gaaagaaatt gcgcaacaaa tgcgtggcga attaactcaa aatggtttta caagtttaga
    41821 aacgagcgaa gcagtatcgg agtatatgaa ccaagtaaat gctgatgaca ctacttttgt
    41881 agttattaac tctacatgcg gctgtgcagc tggattagca agaccagctg cagtagcagt
    41941 tgcaacacaa aatgaacata gacctacaaa tacagttaca gtttttgctg ggcaagataa
    42001 agaagcaact gctacaatgc gagaattcat tcagcaagca ccatctagtc cttcgtatgc
    42061 tttattcaaa ggtcaagatt tagtttattt tatgcctaga gaatttatcg aaggtagaga
    42121 tattaatgac attgcaatgg acttaaagga tgcctttgac gaaaattgta aatagtacac
    42181 ataaataaat ataaaggtta acacatttta taatattaaa aatggtgtct gtc
//
LOCUS       DAKTGS010000007        34398 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.007, whole genome shotgun sequence.
ACCESSION   DAKTGS010000007 DAKTGS010000000
VERSION     DAKTGS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34398
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.007"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            194..514
                     /locus_tag="O0286_000300"
     CDS             194..514
                     /locus_tag="O0286_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCY0168702.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            511..672
                     /locus_tag="O0286_000301"
     CDS             511..672
                     /locus_tag="O0286_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCY0168703.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            767..1027
                     /locus_tag="O0286_000302"
     CDS             767..1027
                     /locus_tag="O0286_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0168704.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            1067..1327
                     /locus_tag="O0286_000303"
     CDS             1067..1327
                     /locus_tag="O0286_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0168705.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            1336..1599
                     /locus_tag="O0286_000304"
     CDS             1336..1599
                     /locus_tag="O0286_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168706.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            1608..3551
                     /locus_tag="O0286_000305"
     CDS             1608..3551
                     /locus_tag="O0286_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0168707.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            3553..4473
                     /locus_tag="O0286_000306"
     CDS             3553..4473
                     /locus_tag="O0286_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCY0168708.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            4554..5171
                     /locus_tag="O0286_000307"
     CDS             4554..5171
                     /locus_tag="O0286_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0168709.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            5172..5642
                     /gene="ssb"
                     /locus_tag="O0286_000308"
     CDS             5172..5642
                     /gene="ssb"
                     /locus_tag="O0286_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0168710.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5672..6565
                     /locus_tag="O0286_000309"
     CDS             5672..6565
                     /locus_tag="O0286_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0168711.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            6572..6790
                     /locus_tag="O0286_000310"
     CDS             6572..6790
                     /locus_tag="O0286_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168712.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6799..7203
                     /locus_tag="O0286_000311"
     CDS             6799..7203
                     /locus_tag="O0286_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCY0168713.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            7216..7584
                     /locus_tag="O0286_000312"
     CDS             7216..7584
                     /locus_tag="O0286_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168714.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            7588..7830
                     /locus_tag="O0286_000313"
     CDS             7588..7830
                     /locus_tag="O0286_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCY0168715.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            7839..8210
                     /locus_tag="O0286_000314"
     CDS             7839..8210
                     /locus_tag="O0286_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0168716.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            8203..8439
                     /locus_tag="O0286_000315"
     CDS             8203..8439
                     /locus_tag="O0286_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0168717.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            8429..8671
                     /locus_tag="O0286_000316"
     CDS             8429..8671
                     /locus_tag="O0286_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168718.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            8664..9197
                     /locus_tag="O0286_000317"
     CDS             8664..9197
                     /locus_tag="O0286_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCY0168719.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            9234..9479
                     /locus_tag="O0286_000318"
     CDS             9234..9479
                     /locus_tag="O0286_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168720.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            9476..9682
                     /locus_tag="O0286_000319"
     CDS             9476..9682
                     /locus_tag="O0286_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0168721.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            9679..10065
                     /locus_tag="O0286_000320"
     CDS             9679..10065
                     /locus_tag="O0286_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168722.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            10062..10211
                     /locus_tag="O0286_000321"
     CDS             10062..10211
                     /locus_tag="O0286_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0168723.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            10211..10411
                     /locus_tag="O0286_000322"
     CDS             10211..10411
                     /locus_tag="O0286_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0168724.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            10439..10855
                     /locus_tag="O0286_000323"
     CDS             10439..10855
                     /locus_tag="O0286_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0168725.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            11087..11386
                     /locus_tag="O0286_000324"
     CDS             11087..11386
                     /locus_tag="O0286_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0168726.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            11515..11859
                     /locus_tag="O0286_000325"
     CDS             11515..11859
                     /locus_tag="O0286_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168727.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            11856..13517
                     /locus_tag="O0286_000326"
     CDS             11856..13517
                     /locus_tag="O0286_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0168728.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            13533..14720
                     /locus_tag="O0286_000327"
     CDS             13533..14720
                     /locus_tag="O0286_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0168729.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            14704..15441
                     /locus_tag="O0286_000328"
     CDS             14704..15441
                     /locus_tag="O0286_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0168730.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            15465..16610
                     /locus_tag="O0286_000329"
     CDS             15465..16610
                     /locus_tag="O0286_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0168731.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            16630..16914
                     /locus_tag="O0286_000330"
     CDS             16630..16914
                     /locus_tag="O0286_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168732.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            16904..17188
                     /locus_tag="O0286_000331"
     CDS             16904..17188
                     /locus_tag="O0286_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCY0168733.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            17172..17534
                     /locus_tag="O0286_000332"
     CDS             17172..17534
                     /locus_tag="O0286_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCY0168734.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            17531..17935
                     /locus_tag="O0286_000333"
     CDS             17531..17935
                     /locus_tag="O0286_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCY0168735.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            17932..18336
                     /locus_tag="O0286_000334"
     CDS             17932..18336
                     /locus_tag="O0286_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168736.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            18340..18984
                     /locus_tag="O0286_000335"
     CDS             18340..18984
                     /locus_tag="O0286_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0168737.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            19011..19250
                     /locus_tag="O0286_000336"
     CDS             19011..19250
                     /locus_tag="O0286_000336"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCY0168738.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            19300..19650
                     /locus_tag="O0286_000337"
     CDS             19300..19650
                     /locus_tag="O0286_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168739.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            19701..19838
                     /locus_tag="O0286_000338"
     CDS             19701..19838
                     /locus_tag="O0286_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168740.1"
                     /translation="MMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDA
                     F"
     gene            19895..24424
                     /locus_tag="O0286_000339"
     CDS             19895..24424
                     /locus_tag="O0286_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0168741.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATILAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEII
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            24421..25905
                     /locus_tag="O0286_000340"
     CDS             24421..25905
                     /locus_tag="O0286_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0168742.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            25921..29706
                     /locus_tag="O0286_000341"
     CDS             25921..29706
                     /locus_tag="O0286_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168743.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            29696..29848
                     /locus_tag="O0286_000342"
     CDS             29696..29848
                     /locus_tag="O0286_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168744.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            29895..30182
                     /locus_tag="O0286_000343"
     CDS             29895..30182
                     /locus_tag="O0286_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168745.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            30240..30536
                     /locus_tag="O0286_000344"
     CDS             30240..30536
                     /locus_tag="O0286_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0168746.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            30686..30862
                     /locus_tag="O0286_000345"
     CDS             30686..30862
                     /locus_tag="O0286_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCY0168747.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(30915..31022)
                     /locus_tag="O0286_000346"
     CDS             complement(30915..31022)
                     /locus_tag="O0286_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168748.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            31074..31328
                     /locus_tag="O0286_000347"
     CDS             31074..31328
                     /locus_tag="O0286_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0168749.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            31340..32095
                     /locus_tag="O0286_000348"
     CDS             31340..32095
                     /locus_tag="O0286_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0168750.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            32286..32777
                     /gene="sak"
                     /locus_tag="O0286_000349"
     CDS             32286..32777
                     /gene="sak"
                     /locus_tag="O0286_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCY0168751.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            33427..33762
                     /locus_tag="O0286_000350"
                     /pseudo
     CDS             33427..33762
                     /locus_tag="O0286_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(33857..34306)
                     /locus_tag="O0286_000351"
     CDS             complement(33857..34306)
                     /locus_tag="O0286_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCY0168752.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
BASE COUNT        13058 a         4804 c         6867 g         9669 t
ORIGIN      
        1 aatgatgtgt agagttttgc tttcagcatc tttcatatgt tcttgtactt taataacttc
       61 accattaatt aaatgtaaat ctaaagaagt aacgatttct ttatctaaat attgagtgag
      121 caatctctta tgcatactta tcacctcctt tcactaggag ataacaacat tatacacgaa
      181 aggaaagata gaaatgccac acattttaaa cgtaacagtt ccaatacctg aaacacatgt
      241 acttatcaca aaagatgaat atgatgagct aattggttat tcattagacc ctgtatggaa
      301 catgagtgac ttaaagaaga aattaaaaat tgcatctgat gagactatca aggacagatt
      361 actatttcat cctagatttg aaaaagaact aagagcgcaa ggaattgtgc attacccaga
      421 tgagagtttt aatcgctgga gatttaacgc aagaaagatg aataaattcg tcgatgagca
      481 tttcaatgaa atatataagg agagaataaa atgagcgaca catataaaag ctacctatta
      541 gcagtgctat gtttcacagt cttagcgatt gtgcttatgc cattgctgta cttcactaca
      601 gcgtggtcaa ttgcaggatt cgcaagcatt ggaacattca tatactacaa agaatacttt
      661 tatgaagaat aaaaaaactg ctacttgcgt caacaagtaa cagtgacaaa cgattaacaa
      721 aattaattca ttttcaatat aaaacgaaac aaggaggaag tcaaccatga ctaaaaatta
      781 taaagatatg actcaagacg aattaagggg tttattggct gaaaagaatg cagaattgtt
      841 tgatttagcg agcgaaatcg atgaagaaac tgaatttgac gttttacttt tttcaaatgt
      901 agggattagc aacggagatt ttacaccgtc gtcacattgt gtgattggga atgttgttga
      961 tattgctaat ttattgaaac gtagagctgt ttaccgagat atcgctgatg ttatcaaaat
     1021 gcgttaaaaa ttctagctat agataatgac aaggaggacg agaacaatgt attacaaaac
     1081 gggtgacgta tgtcgaaaaa taattaatgt agatggcttt gattttcaat taagagttaa
     1141 gaaacgagcg tatagcgtcg aaatggttgt tctagaccat gaaggaaatt caattgacgg
     1201 gttactagtt tctgatgaga acgatctata cacagcttta gatattttga aacaaagtgt
     1261 ttatgaatgg attgaaaata acacagatga acaggacaaa ctaatgaact tagtcatgaa
     1321 atggtaggta taagcatgag agatacagaa agaaatatat tgaatatttt taagacgtta
     1381 ttcgacgaat atactttgtc aaaccaacga gcattattgg aaattgaacg taatcatcac
     1441 ggatacttat cgattaattt cttgcactat cacgacagtt acaaaacaaa caataagctt
     1501 gtgcagatac atgaaatcaa tccagacagc catgaacgaa taaaaaattt aattatcgag
     1561 gtactaagag gtcatcggaa gattaaaaaa ggagcatgag gaaggatatg aaaataaata
     1621 aattaacaat atcgaacttt gccggaatca aagaagaaaa atttaatttt gacggtaaag
     1681 atgcaaaaat atacggcaat aatgcgactg gtaagactac aacagcaacc gcattacaat
     1741 ggttgctttt cgataaaggt ttggacggtt caaccaaatc atttaaccct gtacctttaa
     1801 acgaaaaaaa cgaagaaaat tatgagttaa ttccgactgt tttcgcagaa tttgaaatcg
     1861 acggaaaaat tacgactttt aaaaaagagt cacatcctaa atacacaata aatcaaaaga
     1921 cgaatcgcaa ggaatactca cgaagtcgaa cgaagaaaca atatatcaat gatgaatcaa
     1981 taaaagtaaa ggattataaa gctcgtattg atgaacttat tgatgaagat gtattcaagt
     2041 taattacgaa ccctcaagca tttaacttac tagattggaa gaagcgaaga agtttgttgt
     2101 ttgaaattgc taaaccaatc aatgatgagg atgtcattaa aacaaatgat gattttaaag
     2161 aactaaataa tattcttgga gatcatgaaa ttgaaacaaa gaaaaagatt cttacggaca
     2221 agataaaaca gattaacaaa gatatcaaag atattccgat acgtattaac caaacacaac
     2281 aaaataagca ggatgtacca gaattcgata atgatagata cgcaattatc aaacaagaaa
     2341 ttgagcaact tgaaaatgag cgtatagata ttcaaaacgg taaggaagaa attaatttac
     2401 gtaatcaatt agctgataaa caatcagaat tgaaacgcat agaagacaat aacagcgcaa
     2461 gtaatgagaa caaaatccat gctttaacaa atgaattaca cgttgaaaat ggaacggtag
     2521 caaaccttaa aacgagatta aagcaaaaca aacaacaaat cacgcatgaa gaaaatagac
     2581 gtaatcaatt attagaaaat cataaaggat taaaaagtga tttagaaaaa gctaaaaatc
     2641 aaaaatttga atatcttgat gacaatgtat gtagttgttg tggtcaaaag ttaccagttg
     2701 aacaagtgga tatggcaaga gaaaaagcat tgcagaaatt caatgctagc aaatcgaaag
     2761 aattagaaac aatacaaaca tctatcaatc acattatttc agaaggcaag aaaataaagc
     2821 taatcatcga gaagttagag gatgacaata ataatcttca aattaaaatc aacgaagcag
     2881 aagagcgttc agcaagaata caaaacaaaa ttaataagtt gaaaacaact cacgttgacg
     2941 ttacgcaaac tgacgaatac aaagcagtaa tgttagagat aaatgagatt aatcaaaaac
     3001 gctctaacat caggaaaact attcaagata aagtttcagg aatagatgac aaaataagcg
     3061 aacttactca agaaaaatca gaaattgaag tgtcaagatc aatcgaaaaa tcaaataaac
     3121 atctagatga tgttatttct gaattaagaa atgaagaaga cagattattg gatgaaaaag
     3181 aaaagtattc acatgacctt tatatcttaa aagaatttac aacaacaaaa gtcaaaatgc
     3241 ttactgaaaa catcaataat gaatttgaaa ttgctgaatt taagctattc aataccttag
     3301 ttaacggcga attagaagaa acatgttcca caacggttaa cggcgtcgaa tacgacagcg
     3361 gtttaaataa cgcctcaaga attaatgttg gcttagatat catcaataca ctgtcaaaac
     3421 attttaaagt tacagcgcca atatttattg ataatgctga atcagtaaca gagcttatca
     3481 aaacagaatc acaacaaatt caattgatag taaatgaaca agataaaaaa ttaagaatgg
     3541 agactatata aaatgactga aaataataaa ttacaaacta ttgaacaaca attagtacaa
     3601 gaaaagaacg tatctgacaa cgtattaaac aaagtgagag ttttagagtc acaaggcaat
     3661 ttggaattgc caaatgatta ttcaccaagt aatgccatga aacaagcatg gttacaaatc
     3721 agccaagata acaaattaat gagttgtaac gatacaagca aagcaaatgc cttattagac
     3781 atggtaacgc aaggtttaaa tccagctaaa aatcaatgct actttattcc ttacggcaac
     3841 aaaatgcagt tacaacgtag ctatcacggt aatgtaatga tgttaaaacg tgatgcaggt
     3901 gctcaagatg ttgttgctca agtgatttat aaaggcgata cattcaagca agaaatggga
     3961 gaaacaggac gtatcaaagc gattaaacac gaacaagact tctttaacat cgacaaagaa
     4021 aacattatcg gtgcgtactg cacaatcgta tttaatgatg gacgagataa ctatattgaa
     4081 gtcatgacta ttgaacaaat taaacaagca tggatgcagt catcaatgat taaagatgaa
     4141 aaagcattac aaaattctaa aacacataat aatttcaaag aagaaatggc taaaaaaaca
     4201 gttatcaata gagctgctaa acgttatatc aacacatcaa cagatagcaa tcttttcaaa
     4261 tacgcacaag aatccgaaca acgtcaacgc aaagaagtgt tggacgcaga agttgaagaa
     4321 aatgcaaatc aagaacaatt ggactttgaa caaccagttc ttgaagaagc acaatacaca
     4381 gaattagaaa atgataagcc tattgatgta tctgactttg aagaaataaa agaacctgca
     4441 acagaaaaag aaagcgaaga agagccattt taattgaaac aatagcaact ggttcaagtg
     4501 gtaactgcta cgtcttaaat gatggacgta ctacgttact acttgaggca ggtataaaat
     4561 ttgaacgtgt tcaaaagcat ttcaaatata aaacaagaca tatagcaggg tgtcttatca
     4621 cacacgaaca tggtgatcat gcaaagtata caaagcagtt tgtcgacaat ggtgtaatca
     4681 gctatatgac tgctggaaca caacaagcta tgaattttga aagtcatcgc ttatgcacga
     4741 ttaaggcaaa gcaagagctg cgaataggta catggtcaat tctaccgttt gacatcgaac
     4801 atgatgctaa cgagcctgtg gctttcttat tacaaagcac actaggttat aaggttctgt
     4861 atgttactga tacaaagtat ttgaaataca aatttaatgg cattacgcac atgatgctag
     4921 aagttaatta tatctatgaa caaatgcaag aaaacataaa aaacggcagt gtgcacagca
     4981 cattagcaaa cagaattatg gagtctcatt ttagcttaga acatgctatc ggaatgttaa
     5041 aagcaaatga tttaactaga ctcgaagaaa tacatttaat tcatttaagt agtcaaaatt
     5101 caaatgcaaa atacattaaa agtgaaatac aaaaagtgac gggcgcgccc gtttattttg
     5161 gaggtttata aatgctaaac agaacaatat tagttggtcg tttaactaga gacccagaat
     5221 taagaaccac tcaaaatggt gtaaatgtag catcattcac attagcagtt aaccgtacat
     5281 ttacaaatgc acaaggcgag cgcgaggcag actttattaa tatcatcgta tttaaaaaac
     5341 aagcagagaa cgttaataaa tacctatcta aaggatcgtt ggcgggcgta gatggtaggt
     5401 tacaaacgcg gaactatgaa aataaggaag gtcaacgtgt atacgttacg gaagttattg
     5461 ctgatagtat tcaattttta gaaccgaaaa actcaaatga cactcaacaa gatttatatc
     5521 aacaacaagt acaacaaaca cgtggacaat cgcaatattc aaataacaaa ccagtaaaag
     5581 ataatccgtt tgcgaatgca aatggtccga ttgaactaaa tgatgatgat ttaccattct
     5641 aatttaaccg gtttgaaagt gaggtgtgta tatgactggt tggataagta ttgatcgctc
     5701 aattcaaaat cattggctat ttaaagaaaa gagaacattt tcaaagtttg aagcatggat
     5761 atatttactc atggaagcga atcattcaaa ggcaaaagtg cctataggaa accaaattgt
     5821 aaccgttgaa agaggacaaa gattaacatc tattttgacc ttgtctgacc tttttaactg
     5881 gtcacgattt aaagtgaaaa ccttccttga cttactcgag agtgatggaa tgttagaagt
     5941 caaaacaaca tcaaaatata cccttataac cattgtcaat tatgactttt atcaaagtga
     6001 gcagggcagg aaccaacatc aaaacgacat caaaccaaca tcaaaacaac atcagtcaaa
     6061 catcaaccca acatcaaaac aacatcaaac caacacaaac aataatgata ataaagataa
     6121 taatgaaaag aatgtgaata atgagaagaa gaagacaacc gccttcgact tcttccaaga
     6181 taacggattc ggtttcataa cttcttacaa tttagacgat ttaaattatt atcttgattc
     6241 atttgaaaat gattcagatg aaatagttac cgcatcactt aaaatcgcta aagacagaaa
     6301 caaagttact tggggatatg ctaaaagcat tttgaataca tggcttaatg caaacttgaa
     6361 atctattgaa caagtacgtg catttgaaaa gcaacaactt gaaagcaaaa aacaaaatta
     6421 taaacctttc gttaaacaat caaaagaaaa aacacccaaa tggctcacag acagcacgag
     6481 agaaacgaaa acgccggaag tagatgaaaa ccttgagaaa gacagagaag cttttattaa
     6541 gcgtctaaat agcaaatggg agtgattgaa aatggatgca tttgataaat actatctatt
     6601 tgatcatgac ggcaacaaaa tgttttcagt tacaccacat tttaaagatg gacggcattt
     6661 agttgttgga ttaaaacaca caaaattcaa tggtcgacgt tggtacttag atgattatga
     6721 attaaaaaca cttattgata atgaacaaat ggagttagga caccaaacaa gcttatttga
     6781 atatatatga gggattacat ggagatagaa attaaattta atgaagtgtt taatgcgccg
     6841 atgggctcgc ctcgaccgcg ttttagcaca aaaggtagat atgcacacac atatatgcct
     6901 acaaaatata cagaacataa aaaatattta caaaatcaaa tgccaaagct aaatctagaa
     6961 aatgcattaa aaattgaatt agagttttac tttactccac ctaaatcatg gtcgaagaaa
     7021 aagaaaactc aagcgattgg acaattaaaa gtaactaaac cagatatcga taatttgatg
     7081 aagacagttc tagacgcttg taacaattat ttgtggaaag acgataacca aattacagaa
     7141 ataactagct caaagcgtta tggaattgag cccaaaataa tcatacgaat agaagaaata
     7201 taagaggtgg ataaaatggc gagaaaagca aggattgtaa caataaatga taaaccatat
     7261 aggtttacca aatctgaaat ggaattaata gaaagtcacg gtataaccgc tggaatggtt
     7321 tctaagagag taaaagacgg ttgggaacta catgaagcaa tggacgcacc agaaggtacg
     7381 cgtttaagcg agtacagaga aaagaaaaca atagaaagac tggaacaagc tagactcgaa
     7441 cgcaaattgg aaagaaagcg aaagaaagag gctgagctaa gaagaaagaa gccacatttg
     7501 tttaatgtac cacagaaaca tccaagagga cgttatgcgt gctacctgat ggaaaacgac
     7561 atattcgtga aagttaagaa gtagatcatg acagataacg cacgcaaaga atacctaaat
     7621 caattctttg gatctaagag atatctgtat caggataacg aacgagtggc acatattcat
     7681 gtagtaaacg gcacttatta ctttcatggg catatcgtac caggttggca aggcgtgaaa
     7741 aagacatttg atacagctga agagcttgaa acatatataa agcaacaggg tttggaatat
     7801 gaggaacaga agcaactaac tttattttag aggaggttat gaaagtgaac tatgaaacag
     7861 gattccaaat aggcgtaatg gaagctaggt tgaagaagat gagaaaacaa cgtgatgagt
     7921 acaagaagca acgtgacgag cttattgggg atatagctaa gttaagagag cgtaacgaag
     7981 agctggagaa catgtggcgc acgcttaaaa atgaattgtt tggaagatac gaattttacc
     8041 gttttagact tagcgaacta cagattgaga gcagagcgaa caaggaagta gctatatata
     8101 gaagagctga aatcaactta agtgttatat tgtgccgaat ggacaaacta gacggaacaa
     8161 atgagttcta cgaattttta gggcaaatgg aggatgacac aaatgaataa ccgcgaacaa
     8221 atcgaacaat cagttatcag tgctagcgcg tataacggca atgacacaga gggattgcta
     8281 aaagagattg aagacgtgta taagaaagcg caagcgtttg atgaaattcg cgaagctatt
     8341 aatgcgcaat gggttgagta tccagaagac tgggcgtcag aggttttgag agaagtaaga
     8401 gagcttgaat atgaggagga acaggaaaat gaaaattaat gaatctttga agaaattaaa
     8461 agaaaaagga tacaaagaaa atgaagataa agctattttt aatttagctg atggtacgct
     8521 agaaatttac atcgaccatg acgaaaaaac aataattaca gaattccatg atttaaaggt
     8581 attcgtgtca gaagatttaa aagataaaag tatggagagc gttatgtatg aattggctgg
     8641 aattgatgag gaggacaagg aaaatgacta acatcctaca agtaaagcta ttatcagaaa
     8701 acgctagaat gccagaacga aatcataaga cggatgcagg ttatgacata ttctcagctg
     8761 aaactgtcgt acttgagcca caagaaaagg cagtgatcaa aacagatgta gctgtaagca
     8821 taccagaggg ctatgtcgga ctattaacta gccgtagtgg tgtaagtagt aaaacgcatt
     8881 tagtgattga aacaggcaag atagacgcgg gatatcacgg caatttaggg attaatatca
     8941 agaatgataa tgaaacgtta gagagtgagg atatgagtaa ctttggtcgg agtcctgctg
     9001 ggatagatgg aaagtatgcc cgactacctg taacagataa aattttatgt atgaatggta
     9061 gttatgtcat aaacaaaggc gacaaactag ctcaattggt tattgtgcct atatggacac
     9121 ctgaactaaa gcaagtggag gaattcgaga gtgtttcaga acgtggagca aaaggcttcg
     9181 gaagtagcgg agtgtaaaga catattagat cgagtcaagg aggttttggg gaagtgagcg
     9241 acatgttaga aatattttta atagggtttg gcgtttatct cttttatcgc atagcaatta
     9301 tttttcttaa gagtaaaaag actatacaca caaacatata tgaaatgtta atgcttgcta
     9361 ctatctttat gatatctaca tttgcttata aacatcaaaa gacgcatatc ttaatagcat
     9421 ttttagtaat gttttttatg agtaagctca aacaagttca agggagctat gaggaatgac
     9481 acaatactta gtcacaacat tcaaagattc aacaggacgt aaacatacac acataactaa
     9541 agctaagagt aatcaaaggt ttacagttgt tgaggcagag agtaaagaag aagcgaaaga
     9601 gaagtacgag aaacaagtta aaagggatgc agttattaaa gtgggtcagt tgtttgaaaa
     9661 tataagggag tgtgggaaat gattaaaaaa cttaaaaata tggatgggtt cgacatcttt
     9721 attgttggaa tactgtcatt attcggtata accgcattgc tacttgttgt cgcattgcct
     9781 atctatacag tggctagtta ccaaaacaaa gaagtacatc aagggacaat tacagataaa
     9841 tataacaaaa gacaagataa agaggacaaa ttctatattg tattagatga taaacaagtc
     9901 atcgaaaact cagacttatt cttcaaagga aagtttgata gcgcagacat acaagctagg
     9961 ttaaaagtag gtgataaagt aaaagttaag acgattggat atagaataca ctttttaaat
    10021 ttatatccgg tcttatacga agtaaagaag gtagataaaa aatgattaag caaatactaa
    10081 gattattatt cttactagcg atgtatgagt taggtaagta tgtaactgag caagtatata
    10141 ttatgatgac ggctaatgat gatgtagagg cgccgagtga ttacgtcttt cgagcggagg
    10201 taagtgagtg atgtggatta ctatgactat tgtatttgct atattgctat tagtttgtat
    10261 cagtattaat agtgatcgtg caagggagat acaagcgctc agatatatga atgattatct
    10321 acttgatgaa gtagttaaaa ctaaaggata caacgggtta aaagaataca ggattgaatt
    10381 aaagcgaatg aataacgata ttaaaaagta atttatatta tcggaggtat tacattgaat
    10441 gataaagatt gagaaacacg atatcaaaaa gcttgaagaa tacattcagc acatcgataa
    10501 ctatcgaaga gagttgaaga tgcgagaata tgaattactt gaaagtcatg aaccagataa
    10561 tgcgggagct ggcaaaagta atttgccggg taacccgatt gaacgatgtg caataaagaa
    10621 gtttagtgat aacaggtaca atacattaag aaatatagtt aacggtgtag atagattgat
    10681 agatgaaagt gatgaggata cgcttgagtt attaaggttt agatattggg attgtcctat
    10741 tggttgttat gaatgggaag atatagcaca ttactttggt acaagtaaga caagtatatt
    10801 acgtagaagg aatgcactga tcgataagtt agcaaagtat attggttatg tgtagcggac
    10861 ttttacccta tgtaagtccg cattaaaaca gtttattatg ttagtatcag attaatattt
    10921 aaagttatta aatgctaata cgacgcatga acaagaggcg catcactatg tgatgtgtct
    10981 ttttatttat gaggtatgaa catgttcaaa ctaatagtaa atacattact acacatcaag
    11041 tatagatgcg tcttgatact acttaagtta tataaggtga aacattatga tgactaaaga
    11101 cgaacgcata cgattctata agtctaaaga atggcaaaca acaagaaaaa gagtgctaga
    11161 aagagataat tatgaatgtc aacaatgtaa gagagacggc aagttaacga catatgacaa
    11221 aagcaagcgt aagtcgttgg atgtagatca tatattatcg ctagaacatc atccggagtt
    11281 tgctcatgac ttaaacaatt tagaaacact gtgtattaaa tgtcacaaca aaaaagaaaa
    11341 gagatttata aaaaaagaaa ataaatggaa agacgaaaaa tggtaaatac ccccgggtca
    11401 aaaaaatcaa aagcgatcaa aatacttggg gaacggggag gggctcgact tcgcgataat
    11461 tttaaaaatc catgtatacc cccctcttat aaccatttta aggcaggtga tgaaatggag
    11521 attatagttg atgaaaactt agtgcttaaa gaaaaagaaa ggctgcaagt attatataaa
    11581 gacataccta gcaataaatt aaaagtagtt gatggtttaa ttattcaagc agcaaggcta
    11641 cgtgtaatgc ttgattacat gtgggaagac ataaaagaaa aaggtgacta tgatttattt
    11701 actcaatctg aaaaggcgcc accatatgaa agggaaagac cagtagccaa actatttaat
    11761 gctagagatg ctgcatatca aaaaataatc aaacaattat cggatttatt gcccgaagag
    11821 aaagaagaca cagaaacgcc atctgatgat tacctatgat tagtaataaa tacgttgatg
    11881 aatatataaa tttgtggaaa caaggaaaga taattttaaa taaagaaaga attgatctct
    11941 ttaattatct acaaaaacat atatattcac gagatgatgt atattttgat gaacagaaaa
    12001 tcgaggattg tatcaaattt attgaaaaat ggtattttcc aacattacca tttcaaaggt
    12061 ttatcatagc taatatattt cttatagata aaaatacaga tgaagctttc tttacagaat
    12121 ttgctatttt catgggacgt ggaggcggga aaaacggtct aataagtgct attagtgatt
    12181 ttctttctac gcccttacac ggagttaaag aatatcacat ctccattgtt gctaatagtg
    12241 aagatcaagc aaaaacatcg tttgatgaaa tcagaaccgt tttaatggat aacaaacgaa
    12301 ataagacggg taaaacgcca aaagctcctt atgaagttag taaagcaaaa ataataaacc
    12361 gtgcaactaa atcggttatt cgatataaca catcaaacac aaaaaccaaa gacggtggac
    12421 gtgaggggtg tgttattttt gatgaaattc attatttctt tggtcctgaa atggtaaacg
    12481 tcaaacgtgg tggattaggt aaaaagaaaa atagaagaac gttttatata agtactgatg
    12541 gttttgttag agagggttat atcgatgcaa tgaagcacaa aattgcaagt gtattaagtg
    12601 gcaaggttaa aaatagtaga ttgtttgctt tttattgtaa gttagacgat ccaaaagaag
    12661 ttgatgacag acagacgtgg gaaaaggcga acccaatgtt acataaaccg ttatcagaat
    12721 acgctaaaac actgctaagt acgattgaag aagaatataa cgatttacca ttcaaccgtt
    12781 caaataagcc cgaattcatg actaagcgaa tgaatttgcc tgaagttgac cttgaaaaag
    12841 taatagcacc atggaaagaa atactagcga ctaatagaga gataccaaat ttagataatc
    12901 aaatgtgtat tggtggttta gactttgcaa acattcgaga ttttgcaagt gtagggctat
    12961 tattccgaaa aaacgatgat tacatttggt taggacattc gtttgtaaga caagggtttt
    13021 tggatgatgt caaattagaa cctcctatta aagaatggga aaaaatggga ttattgacca
    13081 ttgtcgatga tgatgtcatt gaaattgaat atatagttga ttggttttta aaggctagag
    13141 aaaaatatgg gcttgaaaaa gtcatagctg ataattatag aactgatatt gtaagacgtg
    13201 cgtttgagga tgctggcata aaacttgaag tacttagaaa tccaaaagca atacatggat
    13261 tacttgcacc acgtatcgat acaatgtttg cgaaacataa cgtaatatat ggagacaatc
    13321 ctttgatgcg ttggtttact aataatgttg cagtaaaggt taaacccgat ggtaataaag
    13381 aatatattaa aaaagatgaa aatagaagaa aaaccgatgg gttcatggct tttgttcacg
    13441 cattatatag agcagacgat atagtagaca aagacatgtc taaagcgctt gatgcattaa
    13501 tgagtataga tttctaatag aggaggtgag acatgagtat tctagaaaag atatttaaaa
    13561 ctaggaaaga tataacatat atgcttgatt tagatatgat agaagatcta tcacaacaag
    13621 cgtatgtgaa acgtttagcg attgatagtt gtattgaatt tgttgcgcga gctgtcgctc
    13681 aaagtcattt taaagtattg gaaggtaata gaattcaaaa gaatgatgtt tactacaagt
    13741 taaatataaa accaaatact gacttatcaa gcgatagttt ttggcaacaa gttatatata
    13801 aactaattta tgataacgag gttttaatcg tagtaagtga cagcaaagaa ttacttatcg
    13861 cagatagctt ttacagagaa gagtacgctt tgtatgatga tatattcaaa gatgtaacgg
    13921 ttaaagatta tacttatcaa cgtactttca caatgcaaga ggtcatatat ttaaagtaca
    13981 acaacaataa agtgacacac tttgtagaaa gtctattcga agattacggg aaaatattcg
    14041 gaagaatgat aggtgcacaa ttaaaaaact atcaaataag agggattttg aaatctgcct
    14101 ctagcgcata tgacgaaaag aatatagaaa aattacaagc gttcacaaat aaattattca
    14161 atacttttaa taaaaatcaa ctagcaatcg cgcctttgat agaaggtttt gattatgagg
    14221 aattatctaa tggtggtaag aatagtaaca tgcctttttc tgaattgagt gagctaatga
    14281 gagatgcaat aaaaaatgtt gcgttgatga ttggtatacc tccaggtttg atttacggag
    14341 aaacagctga tttggaaaag aacacgcttg tatttgagaa gttctgttta acacctttat
    14401 taaaaaagat tcagaacgaa ttaaacgcga aactcataac acaaagcatg tatttgaaag
    14461 atacaagaat agaaattgtc ggtgtgaata aaaaagaccc acttcaatat gctgaagcaa
    14521 ttgacaaact tgtaagttct ggttcattta caaggaatga ggtgcggatt atgttaggtg
    14581 aagaaccatc agacaatcct gaattagacg aatacctgat tactaaaaac tacgaaaaag
    14641 ctaacagtgg tgaaaatgat gaaaaagaaa aagatgaaaa cactttgaaa ggtggtgatg
    14701 aagatgaaag cggagattaa aggcgtcatc gtttccaacg aagataaatg ggtttacgaa
    14761 atgcttggta tggattcgac ttgtcctaaa gatgttttaa cacaactaga atttagtgat
    14821 gaagatgttg atattataat taactcaaat ggtggtaacc tagtagctgg tagtgaaata
    14881 tatacacatt taagagctca taaaggcaaa gtgaatgttc gcatcacagc aatagcagca
    14941 agtgcggcat cgcttatcgc aatggctggt gaccacatcg aaatgagtcc ggttgctaga
    15001 atgatgattc acaatccttc aagtattgcg caaggagaag cgaaagatct aaatcatgct
    15061 gcagaaacat tagaacatgt tggtcaaata atggctgagg catatgcggt tagagctggt
    15121 aaaaacaaac aagaacttgt agaaatgatg gctagggaaa cgtggctaaa tgctgatgaa
    15181 gccattgaac aaggttttgc ggatagtaaa atgtttgaaa acgacaatat gcaaattgta
    15241 gcaagcaata cacaagtgtt atcgaaagat gtattaaatc gtgtaacagc tttggtaagt
    15301 aaaacgccag aggttaacat tgatattgac gcaatagcaa ataaagtaat tgaaaaaata
    15361 aatatgaaag aaaaggaatc agaaatcgat gttgcagata gtaaagtatc agcaaatgga
    15421 ttttcaagat tcctttttta atacaaaaaa taggaggtca taaaatgact ataaatttat
    15481 cggaaacatt cgcaaatgcg aaaaacgaat ttattaatgc agtaaacaac ggtgaaccgc
    15541 aagaaagaca aaatgaattg tacggtgaca tgattaacca actatttgaa gaaactaaat
    15601 tacaagcaaa agcagaagct gaaagagttt ctagtttacc taaatcagca caatctttga
    15661 gtgcaaacca aagaagtttc ttcatggata tcaataaaaa cgttaactat aaagaagaaa
    15721 aacttttgcc agaagaaaca attgatagaa tttttgaaga tttgacgacg aatcatccgt
    15781 tattagctga tttaggtatt aaaaacgctg gtttgcgttt gaagttctta aaatctgaaa
    15841 cttctggcgt agccgtttgg ggtaaaatct atggtgaaat taaaggtcaa ttagatgctg
    15901 cgttcagtga agaaacagca attcaaaata aattgacagc gtttgttgtt ttaccaaaag
    15961 atttaaatga ttttggtcct gcgtggattg aaagatttgt tcgtgttcaa atcgaagaag
    16021 catttgcagt ggcgcttgaa actgcgttct taaaaggtac tggtaaagac caaccaatcg
    16081 gcttaaaccg tcaagtacaa aaaggtgtat cggtaactga gggtgcttat ccagagaaag
    16141 aagaacaagg tacgcttaca tttgctaatc cgcgcgctac agttaatgaa ttgacgcaag
    16201 tgtttaaata ccactcaact aacgagaaag gtaaatcagt agcggttaaa ggtaatgtaa
    16261 caatggttgt taatccgtcc gatgcttttg aggttcaagc acagtataca catttaaatg
    16321 caaatggcgt atatgttact gctttaccat ttaatttgaa tgttatcgag tctacagtcc
    16381 aagaagcagg taaggtttta acgtacgtta aaggtttata tgatggttat ttagctggtg
    16441 gtattaatgt tcagaaattt aaagaaacac ttgcgttaga tgatatggat ttatacactg
    16501 caaaacaatt tgcttacggc aaagcgaaag ataataaagt tgctgctgtt tggaaattag
    16561 atttaaaagg acataagcca gctttagaag gtaccgaaga aacactataa aattttatga
    16621 ggtgataaaa tggtgaaatt taaagttgtt agagctttta aagacataga gcacaatcaa
    16681 cacaagtaca aagtagggga gttgtatcca gctgaagggt ataacaatcc tcgtgttgaa
    16741 ttgttgacaa atcaaatcaa aaataagtac gacaaagttt atatcgtacc tttagataag
    16801 ctgacaaaac aagaattatt agaactatgc gaatcattac aaaaaaaagc gtctagttca
    16861 atggttaaaa gtgaaatcgt cgacttattg aatggtgaag acaatgacga ttgatgattt
    16921 gcttgtcaaa tttaaatcac ttgaaaagat tgaccataat tcagaggatg agtacttaaa
    16981 gcagttgtta aaaatgtcgt acgagcgtat aaaaaatcag tgcggagttt ttgaattaga
    17041 gaatttaata ggtcaagaat tgatacttat acgcgctaga tatgcttatc aagatttatt
    17101 agaacacttc aacgataatt acagacctga aataatagat ttttcgttat ctctaatgga
    17161 ggtatcagaa gatgaagaaa gtgtttaaaa aacctagaat tacaactaaa cgtttaaata
    17221 ctcgtgttca tttttataag tatactgaaa ataatggtcc agaagctgga gaaaaagaag
    17281 aaaaattatt atatagctgt tgggcgagta ttgatggtgt ctggttacgt gaattagaac
    17341 aagctatctc aaacggaacc caaaatgaca ttaaattgta tattcgtgat ccgcaaggtg
    17401 attatttacc cagtgaagaa cattatcttg aaattgaatc aagatatttc aaaaatcgtt
    17461 tgaatataaa gcaagtatca ccagatttgg ataataaaga ctttattatg attcgtggag
    17521 gatatagttc atgagtgtga aagtgatagg tgataaagca ttagaaagag aattagaaaa
    17581 tcgtttcgga actcaagcga tgttaaaagt tcaagatagg gcgctaattg ctggtgctaa
    17641 agtaattgtt gaagaagtaa aaaaacaact aaagccatca aaagatacgg gcgcattaat
    17701 taatgaggta agttttagta aacctgaatg gataaacgga aaacgtacaa ttactgttca
    17761 ttggcgaggt tctaaagacc gttataaaat cgtacattta attgaaaatg gacacgttca
    17821 aaaagggaca ggtaaattta tcaaacctaa agctatgggt ggcgttaata gagcaataag
    17881 acaagggcaa aataagtatt ttgagacgct aaaaagggag ttaaaaaaat tgtgattgat
    17941 attttgtaca aagttcatga agtgattagt caagacagaa ttattagaga gcacgtaaat
    18001 atcaataata ttaagttcaa taaataccca aacgtaaaag atacggatgt acctttcata
    18061 gttattgacg atatagacga cccaatacct acaacttata ctgacggaga tgagcgcgca
    18121 tatagttaca ttgtacaaat agatgtattt gtgaagttta acgaaaagta taacgcgaga
    18181 atcataagaa ataagatatc taatcgtatt caaaagttat tatggtctga actgaaaatg
    18241 ggaaatgtat ccaacggaaa acctgaatat atagaagaat ttaaaacata tagaagcact
    18301 cgtgtctacg agggcatttt ttacgaggag gaataataaa tggtagtaaa acaagcaagt
    18361 gcgccgaaag cgtattttaa cattactggt ttaggtttcg ctaaattaac aaaagaaggc
    18421 gcaaagctag aatatagcga tattacaaaa actagaggat tacaaaaaat cggtgttgaa
    18481 actggaggag acttgaaaac agcatatgct gatggtggtc caatcgaatc agggaacaca
    18541 gacggagaag gtaaaatttc gttacaaatg catgctttcc ctaaagagat tcgcaaaata
    18601 gtattcaatg aagagtatga tgaatacggt gtttataaag aaactcaagg taaacaaaac
    18661 aactatgtag caatttggtt cagacaagag cgtcgagacg gcacatttag aacggtttta
    18721 ttacctaaag tcatgtttac aaatcctaaa atcgatggcg aaacggctga gaaagattgg
    18781 gatttctcaa gcgaagaggt tgaaggtgag gcacttttcc ctttaattga taataaaaag
    18841 tcagtacgta aatatatctt tgattcagca aacatgacaa atcatagtgg aaatggtgaa
    18901 aaaggcgaag aggctttctt aaagaaaatt ttaggcgaag aatatactgg aaacgtgaca
    18961 gaggataacg gcgaaacttt gtaacgcaac cggcttcatc ggaaactgag ataaagtcgg
    19021 ttaatatacc agatagtttt aaaacactaa aagttggcaa cacatatgat ttaaatgttg
    19081 tagtagagcc atctaatcaa agtaagttat tgaaatacac aacagatcaa acgaatattg
    19141 tatcaatcaa tagtgatggt caagtcactg cggaagcaca aggtattgcc acggttaaag
    19201 catatgttgg taatatgagt gacactataa caataaatgt agaagcataa gaggggacaa
    19261 cccctctatt ttatttgaaa ataaggagag tattataaaa tggcaaaatt aaaacgtaac
    19321 attattcaat tagtagaaga tccaaaagca aatgaaatta aattacaaac gtacttaaca
    19381 ccacacttca tttcatttga aattgtatac gaagcaatgg atttaatcga tgatattgag
    19441 gacgaaaata gcacgatgaa gccaagagaa atcgctgaca gattgatgga tatggttgta
    19501 aaaatttacg ataaccaatt cgcagttaaa gacctaaaag aacgtatgca tgcacctgat
    19561 ggaatgaatg cacttcgtga acaagtggtt ttcattacgc aaggtcaaca aactgaggaa
    19621 actagaaatt ttatccagaa catgaaataa agcctgaaga tttaacatat aaagcaatgt
    19681 tgaaaaatat ggatactctc atgatggact taattgaaaa tggtaaagac gctaacgaag
    19741 ttttaaaaat gccatttcat tatgtgcttt ccatatatca aaataaaaac aatgacattt
    19801 ctgaagaaaa agcagaggct ttaattgatg cattttaacc ttaaccgttt ggttagggtt
    19861 atttttttga acttttttag aaaggaggta aaaaatggga gaaagaataa aaggtttatc
    19921 tataggtttg gatttagatg cagcaaattt aaatagatca tttgcagaaa tcaaacgaaa
    19981 ctttaaaact ttaaattctg acttaaaatt aacaggcaac aacttcaaat ataccgaaaa
    20041 atcaactgat agttacaaac aaaggattaa agaacttgat ggaactatca caggttataa
    20101 gaaaaacgtt gatgatttag ctaagcaata tgacaaggta tctcaagaac agggcgaaaa
    20161 cagtgcagga gctcaaaagt tacgacaaga atataacaaa caagcaaatg aactgaatta
    20221 tttagaaaga gaattacaaa aaacatcagc cgaatttgaa gagttcaaaa aagctcaagt
    20281 tgaagctcaa agaatggcag aaagtggctg gggaaaaacc agtaaagttt ttgaaagtat
    20341 gggacctaaa ttaacaaaaa tgggtgatgg tttaaaatcc attggtaaag gcatgatgat
    20401 tggtgtaact gcacctgttt taggtatcgc agcagcatca ggaaaagctt ttgcagaagt
    20461 tgataaaggt ttagatactg ttactcaagc aacaggcgca acaggcagtg aattaaaaaa
    20521 attgcagaac tcatttaaag atgtttatgg caattttcca gcagatgctg aaactgttgg
    20581 tggagtttta ggagaagtta atacaagatt aggttttaca ggtaaagaac ttgaaaatgc
    20641 cacagagtca ttcttgaaat tcagtcatat aacaggttct gacggtgtgc aagccgtaca
    20701 gttaattacc cgtgcaatgg gcgatgcagg tatcgaagca agtgaatatc aaagtgtttt
    20761 ggatatggta gcaaaagcgg cgcaagctag tgggataagt gttgacacat tagctgatag
    20821 tattactaaa tacggcgctc caatgagagc tatgggcttt gagatgaaag aatcaattgc
    20881 tttattctct caatgggaaa agtcaggcgt taatactgaa atagcattca gtggtttgaa
    20941 aaaagctata tcaaattggg gtaaagctgg taaaaaccca agagaagaat ttaagaagac
    21001 attagcagaa attgaaaaga cgccggatat agctagcgca actattttag cgattgaagc
    21061 atttggtgca aaggcaggtc ctgatttagc agacgctatt aaaggtggtc gctttagtta
    21121 tcaagaattt ttaaaaacta ttgaagattc ccaaggcaca gtaaaccaaa catttaaaga
    21181 ttctgaaagt ggctccgaaa gatttaaagt agcaatgaat aaattaaaat tagtaggtgc
    21241 tgatgtatgg gcttctattg aaagtgcgtt tgctccagtc atggaagaat taataaaaaa
    21301 gctatcagtc gctgtcgatt ggttttcgaa tttaagtgat ggttctaaaa gatcaattgt
    21361 tattttcggt ggtattgctg ctgcaattgg tcctgtagtt tttgggttag gtgcatttat
    21421 aagcacaatt ggtaatgcag taactgtatt agccccacta ttagctggta ttgcaaaggc
    21481 tgatggatta attagttttt tatcgactaa agtacctata ttaggaactg tcttcacagc
    21541 tttaactggt ccaattggca ttgtattagg tgttttagct ggcctagcag tcgcatttac
    21601 aattgcttat aagaaatctg aaacatttag aaattttgtt aatgatgcaa ttgaaagtgt
    21661 taaacaaaca tttagtaatt ttattcaatt tattcaacct ttcattgatt ccgttaaaaa
    21721 cgtctttaaa caagcggttt cagcaatcgt tgatttcgct aaagatattt ggagtcaaat
    21781 taatggattc tttaatgaaa acggaatttc tattgttcaa gcgcttcaaa atatatgcaa
    21841 ttttatcaaa gttatatttg aatttatctt aaattttgta attaaaccaa taatgtttgc
    21901 aatctggcaa gtgatgcaat ttatttggcc ggcggttaaa gccttgattg tcagtacttg
    21961 ggagaatata aaaggtgtga tacaaggaac tttaaatatc atactaggtt taattaagtt
    22021 cttctcaagt ttatttactg gagattggcg aggagtttgg gacgcgatta ttatgattct
    22081 taaaggagtc gttcaattaa tatggaattt aattcaatta tggtttgtag gcaaaatact
    22141 tggcgttgtt aggtactttg gcggattgct aaaaggatta ataacaggta tttgggacgt
    22201 aataaaaagt atattcagta aatctttatc agcaatttgg aatgcgacaa aaagtatttt
    22261 tggattctta tttaatagtg tcaaatcaat tttcacgaat atgaaaaatt ggttatctaa
    22321 cacatggagt agtatccgta cgaatacgat aggaaaagca cagtcattat ttagtggtgt
    22381 gaaatcaaaa ttcactagtt tgtggaatgc gacaaaagat atttttagta atctaagaaa
    22441 ttggatggca aatatttgga attccattaa agataatacg gttggaattg caagtcgttt
    22501 atggagtaaa gtgcgtggaa tttttacaaa tatgcgtgac ggcttacaaa gtattatcag
    22561 caaaattaaa agtcatatcg gcggtatggt agatgctatt aaaaaaggac ttaataaatt
    22621 aattgaaggt ttaaactggg tcggtggtaa gctgggaatg gataaaatac ctaagttaca
    22681 cactggtaca gaacacacac atactactac aagattagtt aagaacggta agattgcgcg
    22741 ggatacgttc gctacggttg gggataaagg acgtggaaat ggtccgaatg gtttcagaaa
    22801 tgaaataatt gaattcccta atggtaaacg tgtaatcaca cctaatacag atactaccgc
    22861 ttatttacct aaaggctcaa aagtatacaa cggtacacaa acttattcaa tgttaaacgg
    22921 aacgcttcca agatttagct taggtactat gtggaaagat attaaatccg gtgcatcatc
    22981 ggcatttaac tggacaaaag atcaaatagg taaaggtacc aaatggcttg gcgataaagt
    23041 tggcgatgtt ttagatttta tggaaaatcc tggcaaactt ttaaattata tacttgaagc
    23101 ttttggaatt gatttcaatt ctttaactaa aggtatggga attgcaggcg acataacaaa
    23161 agctgcatgg tctaagatta agaaaagtgc tactgattgg ataaaagaaa atttagaagc
    23221 tatgggcggt ggcgatttag tcggcggaat attagaccct gacaaaatta attatcatta
    23281 tggacgtacc gcagcttata ctgctgcaac tggaagacca tttcatgaag gtgtcgattt
    23341 tccatttgta tatcaagaag ttagaacgcc gatgggtggc agacttacaa gaatgccatt
    23401 tatgtctggt ggttatggta attatgtaaa aattactagt ggagttatcg atatgctatt
    23461 tgcgcatttg aaaaacttta gcaaatcacc acctagtggc acgatggtaa agcccggcga
    23521 tgttgttggt ttaactggta ataccggatt tagtacagga ccacacttac attttgaaat
    23581 gagaagaaat ggacgtcact ttgaccctga accatattta agaaatgcaa agaaaaaagg
    23641 taggttatca attggtggcg gtggtgctac ttctggaagt ggtgcaactt atgcaagccg
    23701 agtaatccgt caagcgcaaa gtattttagg aggacgttat aaaggtaagt ggattcatga
    23761 ccagatgatg cgagttgcaa agcgtgaaag taactatcaa tcaaatgcag tgaataattg
    23821 ggatattaat gctcaaagag gagacccatc aagaggatta ttccaaatca tcggctcaac
    23881 ttttagagca aacgctaaac gtggatatac taactttaat aatccggttc atcaaggtat
    23941 ttcagcaatg cagtacattg ttagacgcta cggttgggat ggatttaagc gtgctggtga
    24001 ttacgcatat gctacaggtg gaaaagtttt tgatggttgg tataacttag gtgaagacgg
    24061 tcatccagaa tggattattc caacagatcc agctcgtaga aatgatgcaa tgaagatgtt
    24121 gcattatgca gcagcagaag taagagggag aaaagcgagt aaaaataagc gtcctagtca
    24181 attgtctagt gtaaatgggt ttgatgaccc aagcttatta ttgaaaatga ttgaacaaca
    24241 gcaacaacaa atagctttat tactgaaaat agcgcaatcc aacgatgtga ttgcagataa
    24301 agattatcag ccgattattg acgaatacgc ttttgataaa aaggtgaacg cgtctataga
    24361 aaagcgagaa aggcaagaat caacaaaagt aaagtttaga aaaggaggaa ttgctattca
    24421 atgatagaca ctattaaagt gaacaacaaa acaattcctt ggttgtatgt cgaaagaggg
    24481 tttgaaatac cctcttttaa ttatgtttta aaaactgaaa atgtagatgg acgttcgggg
    24541 tctatatata aagggcgtag gcttgaatct tatagttttg atataccttt ggtggtacgt
    24601 aatgactatt tatctcacaa cggctttaaa acacatgatg acgtcttgaa tgaattagta
    24661 aagtttttta actacgagga acaagttaaa ttacaattca aatctaaaga ttggtactgg
    24721 aacgcttatt ttgaaggacc aataaagctg cacaaagaat ttgcaatacc tgttaagttc
    24781 actatcaaag tagtactaac agacccttac aaatattcag taacaggata taaaaatact
    24841 gcgatttcag accaagtttc agttgtaaat agtgggactg ctgacactcc tttaattgtt
    24901 gaagcccgag caattaaacc atctagttac tttatgatca ctaaaaatga tgaagattat
    24961 tttatggttg gtgatgatga ggtaaccaaa gaagttaagg attacatgcc tcctgtttat
    25021 catagtgagt ttcgtgattt caaaggttgg actaagatga ttactgaaga tattccaagt
    25081 aatgatttag gtggtaaagt cggcggtgac tttgtgatat ccaatcttgg cgaaggatat
    25141 aaagcaacta atttttctga tgcaaaaggt tgggttggtg ctggcacgaa acgagggctc
    25201 cctaaagcga tgacagattt tcaaattacc tataaatgta ttgttgaaca aaaaggtaaa
    25261 ggtgccggaa gaacagcaca acatatttat gatagtgatg gtaagttact tgcttctatt
    25321 ggttatgaaa ataaatatca tgatagaaaa ataggacata ttgttgttac gttgtataac
    25381 caaaaaggag accccaaaaa gatatacgac tatcagaata aaccgataat gtataacttg
    25441 gacagaatcg ttgtttatat gcggctcaga agagtaggta ataaattttc tattaaaact
    25501 tggaaatttg atcacattaa agacccagat agacgtaaac ctattgatat ggatgagaaa
    25561 gagtggatag atggcggtaa gttttatcag cgtccagctt ctatcatagc tatctatagt
    25621 gcgaagtata atggttataa gtggatggag atgaatggat taggttcatt caatacggag
    25681 attctaccaa aaccgaaagg cgcaagggat gtcattatac aaaaaggtga tttagtaaaa
    25741 atagatatgc aagcaaaaag tgttgtcatc aatgaggaac caatgttgag cgagaaatcg
    25801 tttggaagta attatttcaa tgttgattct gggtacagtg aattaatcat acaacctgaa
    25861 aacgtctttg atacgacggt taaatggcaa gatagatatt tatagaaagg agatgagagt
    25921 gtgatacatg ttttagattt taacgacaag attatagatt tcctttctac tgatgaccct
    25981 tccttagtta gagcgattca taaacgtaat gttaatgaca attcagaaat gcttgaactg
    26041 ctcatatcat cagaaagagc tgaaaagttc cgtgaacgac atcgtgttat tataagggat
    26101 tcaaacaaac aatggcgtga atttattatt aactgggttc aagatacgat ggacggctac
    26161 acagagatag aatgtatagc gtcttatctt gctgatataa caacagctaa accgtatgca
    26221 ccaggcaaat ttgagaaaaa gacaacttca gaagcattga aagatgtgtt gagcgataca
    26281 ggttgggaag tttctgaaca aaccgaatac gatggcttac gtactacgtc atggacttct
    26341 tatcaaacta gatatgaagt tttaaagcaa ttatgtacaa cctataaaat ggtattggat
    26401 ttttatatag agcttagttc taataccgtc aaaggtagat atgtggtact caaaaagaaa
    26461 aacagcttat tcaaaggtaa agaaattgag tatggtaaag atttggttgg gttaactagg
    26521 aagattgata tgtcagaaat caaaacagca ttaattgctg tgggacccga aaatgacaaa
    26581 ggaaagcgtt tagagctagt tgtgacagat gacgaagcgc aaagtcaatt caacctacct
    26641 atgcgctata tttgggggat atatgaacca caatcagatg atcaaaatat gaatgaaaca
    26701 cgattaagtt ctttagccaa aacagagtta aataaacgta agtcggcagt tatgtcatat
    26761 gagattactt ctactgattt ggaagttacg tatccgcacg agattatatc aattggcgat
    26821 acagtcagag taaaacatag agattttaac ccgccattgt atgtagaggc agaagttatt
    26881 gctgaagaat ataacataat ttcagaaaat agcacatata cattcggtca acctaaagag
    26941 ttcaaagaat cagaattacg agaagagttt aacaagcgat taaacctaat acaccaaaaa
    27001 ttaaacgaca atattagcaa tatcaatact atagtaaaag atgttgtaga tggtgaatta
    27061 gaatactttg aacgcaaaat tcataaaagt gatacaccgc cagaaaatcc agtcaatgat
    27121 acgctttggt atgatacaag taaccctgat gttgctgtct tgcgtagata ttggaatggt
    27181 cgatggattg aagcaacacc aaatgatgtt gaaaaattag gtggtataac aagagagaaa
    27241 gcgctattca gtgaattaaa caatattttt attaatttat ctatacaaca cgctagtctt
    27301 ttgtcagaag ctacagaatt actgaatagc gagtacttag tagataatga tttgaaagcg
    27361 gacttacaag caagtttaga cgctgtgatt gatgtttata atcaaattaa aaataattta
    27421 gaatctatga cacccgaaac tgcaacgatt ggtcggttgg tagatacaaa aactttattt
    27481 cttgagtata gaaagaaatt acaagatgtt tatacagatg tagaagatgt caaaatcgcc
    27541 atttcagata gatttaaatt attacagtca caatacactg atgaaaaata taaagaagcg
    27601 ttggaaataa tagcaacaaa atttggttta acggtgaatg aagatttgca gttagtcgga
    27661 gaacctaatg ttgttaaatc agctattgaa gcagctagag aatccacaaa agaacaatta
    27721 cgtgactatg taaaaacatc ggactataaa acagacaaag acggtattgt tgaacgttta
    27781 gatactgctg aagctgagag aacgacttta aaaggtgaaa tcaaagataa agttacgtta
    27841 aacgaatatc gaaacggatt ggaagaacaa aaacaatata ctgatgacca gttaagtgat
    27901 ttgtccaata atcctgagat taaagcaagt attgaacaag caaatcaaga agcgcaagaa
    27961 gctttaaaat catacattga tgctcaagat aatcttaaag agaaggaatc gcaagcgtat
    28021 gctgatggta aaatttcgga agaagagcaa cgcgctatac aagatgctca agctaaactt
    28081 gaagaggcaa aacaaaacgc agaactaaag gctagaaacg ctgaaaagaa agctaatgct
    28141 tatacagaca acaaggtcaa agaaagcaca gatgcacaga ggagaacact gactcgctat
    28201 ggttctcaaa ttatacaaaa tggtaaggaa atcaaattaa gaactactaa agaagagttt
    28261 aatgcaacca atcgtacact ttcaaatata ttaaacgaga ttgtccaaaa cgttacagat
    28321 ggaacaacaa tcagatatga tgataacgga gtggctcaag ctttaaatgt ggggccacgt
    28381 ggtattagat taaatgctga taaaattgat attaacggta atagagaaat aaaccttctt
    28441 atccaaaata tgcgagataa agtagataaa accgatattg tcaacagcct taatttatca
    28501 agagagggtc ttgatatcaa tgttaataga attggaatta aaggcggtaa caataacaga
    28561 tatgttcaaa tacagaatga ttctattgaa ctaggtggta ttgtgcaacg aacttggaaa
    28621 ggcaaacgat caaccgatga tatattcaca cgtcttaaag atggacatct aaggtttaga
    28681 aataataccg caggcggttc actttatatg tcacattttg gtatttcaac atatattgat
    28741 ggagaaggcg aagacggagg ttcatccggt actattcaat ggtgggataa aacttacagt
    28801 gatagcggta tgaatggcat aacaatcaat tcctatggtg gtgtcgttgc actaacgtca
    28861 gataataatc gggttgttct ggagtcttac gcttcatcga atatcaaaag caaacaggca
    28921 ccggtgtatt tatatccaaa cacagacaaa gtgcctggat taaaccgatt tgcattcacg
    28981 ctgtctaatg cagataatgc ttattcgagt gacggttata ttatgtttgg ttctgatgag
    29041 aactatgatt acggtgcggg tatcaggttt tctaaagaaa gaaataaagg tcttgttcaa
    29101 attgttaatg gacgatatgc aacaggtgga gatacaacaa tcgaagcagg gtatggcaaa
    29161 tttaatatgc tgaaacgacg tgatggtaat aggtatattc atatacagag tacagaccta
    29221 ctgtctgtag gttcagatga tgcaggagat aggatagctt ctaactcaat ttatagacgt
    29281 acttattcgg ccgcagctaa tttgcatatt acttctgctg gcacaattgg gcgttcgaca
    29341 tcagcgcgta aatacaagtt atctatcgaa aatcaatata acgatagaga tgaacaactg
    29401 gaacattcaa aagctattct taacttacct attagaacgt ggtttgataa agctgagtct
    29461 gaaattttag ctagagagct gagagaagat agaaaattat cggaagacac ctataaactt
    29521 gatagatacg taggtttgat tgctgaagag gtggagaatt taggattaaa agagtttgtc
    29581 acgtatgatg acaaaggaga aattgaaggt atagcgtatg atcgtctatg gattcatctt
    29641 atccctgtta tcaaagaaca acaactaaga atcaagaaat tggaggagtc aaagaatgca
    29701 ggataacaaa caaggattac aagctaatcc tgaatataca attcattatt tatcacagga
    29761 aattatgagg ttaacacaag aaaacgcgat gttaaaagcg tatatacaag aaaataaaga
    29821 aaatcaacaa tgtgctgagg aagagtaatc tttagcacta tttttataca aaaatttaag
    29881 gaggtcattt aattatggca aaagaaatta tcaacaatac agaaaggttt attttagtac
    29941 aaatcgacaa agaaggtaca gaacgtgtag tatatcaaga tttcacagga agttttacaa
    30001 cttctgaaat ggttaaccat gctcaagatt ttaaatctga agaaaacgct aagaaaattg
    30061 cggagacgtt aaatttgtta tatcaattaa ctaacaaaaa acaacgtgtg aaagtagtta
    30121 aagaagtagt tgaaagatca gatttatctc cagaggtaac agttaacact gaaacagtat
    30181 gaaaagctat gagttagata ctcataatct ttattctttt agaaagcggg tgtactgaat
    30241 tggggtggtt caaaaaacac gaacatgaat ggcgcatcag aaggttagaa gagaatgata
    30301 aaacaatgct cagcacactc aatgaaatta aattaggtca aaaaacccaa gagcaagtta
    30361 acattaaatt agataaaacc ttagatgcta ttcaaaaaga aagagaaata gatgaaaaga
    30421 ataagaaaga aaatgataag aacatacgtg atatgaaaat gtgggtgctt ggtttagttg
    30481 ggacaatatt tggatcgcta attatagcat tattgcgtat gcttatgggc atataagaga
    30541 ggtgaataaa atgtttaaac taatctttgg ttatagtttc tggacatgtt tttggttcgg
    30601 taaatgtaaa taagttttag tcagtgcttc ggtactgact ttttatttat tgttgtaatt
    30661 atggtaatat gcagaagtga gcaagttgga tagatggtgg ctatctgagt ataaggaggt
    30721 ggtgcctatg gtggcattac tgaaatcttt agaaaggaga cgcctaatga ttacaattag
    30781 taccatgttg cagtttggtt tattccttat tgcattgata ggtctagtaa tcaagcttat
    30841 tgaattaagc aataaaaaat aaccatcgct aactttggct ggtttcgatg gttaaatggt
    30901 tattaattta atctttaatc taaaatagcc accgtctttt taacgggctc attagggtaa
    30961 catgtttgcg catgttgccc tttttctata tataaattaa cacaccataa tataaatatc
    31021 aaatagacgg cttattagtc gtctttttat tttgggtaaa aggagataag aatatgatta
    31081 attggaaaat tagaatgaaa caaaaatcat tttgggtagc gatattgtca gctatctttt
    31141 tatttgctca aaacatcgca aaagctattg ggtatgatat ccaagtttat acagagcaat
    31201 taacagacgg tttaaacgct atattaggat ttttagtatt aactggtgtg attcaagacc
    31261 cgactactaa aggtataggt gatagccacc aagctttaga atatgaagaa ccaagaagaa
    31321 aatactagga ggtaaaataa tgaaaacata cagtgaagca agagcaaggt tacgttggta
    31381 tcaaggtaga tatattgatt ttgacggttg gtatggttac caatgtgcag atttagcagt
    31441 tgattacatt tattggttgt tagaaattag aatgtgggga aatgcaaaag atgcaatcaa
    31501 taacgatttt aaaaacatgg caacagtata tgaaaacaca ccatcgtttg ttccacaaat
    31561 aggtgatgtg gctgtattta ccaaaggaat atataaacaa tacggtcata ttggtttagt
    31621 gtttaatggt ggtaatacaa accaattttt aattttggaa cagaactatg acggtaacgc
    31681 aaatacgcct gcaaagttac gttgggataa ttattacggc tgtactcact ttattagacc
    31741 taagtataaa agtgagggct taatgaataa gatcacaaat aaagttaaac cacctgctca
    31801 aaaagcagtc ggtaaatctg caagtaaaat aacagttgga agtaaagcgc cttataacct
    31861 taaatggtca aaaggtgctt attttaatgc gaaaatcgac ggcttaggtg ctacttcagc
    31921 cactagatac ggtgataatc gtactaacta tagattcgat gttggacagg ctgtatacgc
    31981 gcctggaaca ttaatatatg tgtttgaaat tatagatggt tggtgtcgca tttattggaa
    32041 caatcataat gagtggatat ggcatgagag attgattgtg aaagaagtgt tttaattctt
    32101 aggttaaaat gttaaatatt tgttaattat tttttaatgt aattttagtt tcttttaata
    32161 ttttattgat ttttaatatt ttctcaatat aaaatgaagt tgttgatatt tatcatctta
    32221 aataagggtg ttagctataa aaagagataa ataaaaacaa atatattata tttggaggaa
    32281 gcgccatgct caaaagaggt ttattatttt taactgtttt attgttatta ttctcatttt
    32341 cttcaattac taatgaggta agtgcatcaa gttcattcga caaaggaaaa tataaaaaag
    32401 gcgatgacgc gagttatttt gaaccaacag gcccgtattt gatggtaaat gtgactggag
    32461 ttgatggtaa aggaaatgaa ttgctatccc ctcattatgt cgagtttcct attaaacctg
    32521 ggactacact tacaaaagaa aaaattgaat actatgtcga atgggcatta gatgcgacag
    32581 catataaaga gtttagagta gttgaattag atccaagcgc aaagatcgaa gtcacttatt
    32641 atgataagaa taagaaaaaa gaagaaacga agtctttccc tataacagaa aaaggttttg
    32701 ttgtcccaga tttatcagag catattaaaa accctggatt caacttaatt acaaaggtta
    32761 ttatagaaaa gaaataaaac aaaatagttg tttattatag aaagcaatgt cttgattgaa
    32821 tatgtgtagt gaaaattatc tttcatcaaa ttctcattca tgcacgaatg gttcttcccc
    32881 acctaatcag atattaggtg acttatgggg agaaatcagt taggatgaaa aagtggataa
    32941 tcctttttta ggcaggtact tcggtacttg cctatttttt tatgttataa tctttctaga
    33001 cgtattcaag ggacgtcttt ttagattgta tgttatagct agctttcggg ctagtttttt
    33061 gttatgatgt gttacacatg catcaactat ttacatctat ccttgttcac ccaagcatgt
    33121 cactgggtgt tttttcttat gatagagagc atagttttca tactactccc tcgtagtata
    33181 tatgacttta gcattcccgt ataatagttt acggggtgct ttttatgtta taattaactg
    33241 tatatagtag gagtgaacta tatagcctgt taagtggcct agtaacctaa cacttatcct
    33301 gcaattgata tcctttttgc ccttcactcg atacatatat ctcaacaaca tagaaatatt
    33361 acagtcgcta caccgcatct taaatggtgt ggttattttt attggaagtg tgtatcaggt
    33421 atcagtaatg ttaaaacacc agctaaaaat gaaaagaatt caccagtgcc agcaggttat
    33481 acactcgata aaaacaatgt accgtataaa aaagagactg gttattacac agttgccaat
    33541 gttaaaggta ataacgtgag ggatggctat tcaactaatt caagaattac aggtgtatta
    33601 cccaataacg caactatcaa atatgacggc gcatattgca ttaatggcta tagatggatt
    33661 acttatattg ctaatagtgg acaacgtcgt tatatagcga caggagaggt agacaaggca
    33721 ggtaatagaa taagcagttt tggtaagttt agtgcagttt gataattaga tatataaagg
    33781 tttggcaagt tatgaaatgt ctgccaaacc tttatataaa aaagaaatat ctacctttta
    33841 atttatgtaa ctactattag tatgcatatt cattagtttt tccaggacca ttaattacat
    33901 aagatgattt agactctcct tttttaaaga agtatgtttt atacatttta cctagtaact
    33961 caacattttt tctatcttca gcaagtggtg tattcagata tactgtatag taacctttat
    34021 tttcagttaa aataaccatt ttttcaaatt gagcagaatt ttttgtgcct ttctttaaat
    34081 aatttctcaa acgttcatct aattttccta gcgttgtagg aagaccacta tttttaaatg
    34141 attctttata agctttttct ttctctaaca ttttcttatt tgattctatt tcttcatttg
    34201 taggaaacgg ttcaaaagta aaagctttcg ctgaatgatg gtgtgtactg attcctgccg
    34261 ttaaaaaact taatgctaaa actgttgttg ctaatttctt tttcataatg atgttaattc
    34321 tccttaaatt ctatatttaa atttatagtt agttttgcga aattcctaaa atgagtttaa
    34381 tctaatcgac gaaatata
//
LOCUS       DAKTGS010000008        33504 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.008, whole genome shotgun sequence.
ACCESSION   DAKTGS010000008 DAKTGS010000000
VERSION     DAKTGS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.008"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            431..631
                     /locus_tag="O0286_000352"
     CDS             431..631
                     /locus_tag="O0286_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0168753.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(758..1327)
                     /locus_tag="O0286_000353"
     CDS             complement(758..1327)
                     /locus_tag="O0286_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0168754.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(1587..1982)
                     /locus_tag="O0286_000354"
     CDS             complement(1587..1982)
                     /locus_tag="O0286_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0168755.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(2050..2403)
                     /locus_tag="O0286_000355"
     CDS             complement(2050..2403)
                     /locus_tag="O0286_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0168756.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(2856..3695)
                     /gene="noc"
                     /locus_tag="O0286_000356"
     CDS             complement(2856..3695)
                     /gene="noc"
                     /locus_tag="O0286_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCY0168757.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(3738..4457)
                     /gene="rsmG"
                     /locus_tag="O0286_000357"
     CDS             complement(3738..4457)
                     /gene="rsmG"
                     /locus_tag="O0286_000357"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCY0168758.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(4457..6334)
                     /gene="mnmG"
                     /locus_tag="O0286_000358"
     CDS             complement(4457..6334)
                     /gene="mnmG"
                     /locus_tag="O0286_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCY0168759.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(6401..7780)
                     /gene="mnmE"
                     /locus_tag="O0286_000359"
     CDS             complement(6401..7780)
                     /gene="mnmE"
                     /locus_tag="O0286_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCY0168760.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(7919..8272)
                     /gene="rnpA"
                     /locus_tag="O0286_000360"
     CDS             complement(7919..8272)
                     /gene="rnpA"
                     /locus_tag="O0286_000360"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCY0168761.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(8393..8530)
                     /gene="rpmH"
                     /locus_tag="O0286_000361"
     CDS             complement(8393..8530)
                     /gene="rpmH"
                     /locus_tag="O0286_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCY0168762.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            9261..10622
                     /gene="dnaA"
                     /locus_tag="O0286_000362"
     CDS             9261..10622
                     /gene="dnaA"
                     /locus_tag="O0286_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCY0168763.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            10900..12033
                     /gene="dnaN"
                     /locus_tag="O0286_000363"
     CDS             10900..12033
                     /gene="dnaN"
                     /locus_tag="O0286_000363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCY0168764.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            12414..12659
                     /gene="yaaA"
                     /locus_tag="O0286_000364"
     CDS             12414..12659
                     /gene="yaaA"
                     /locus_tag="O0286_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCY0168765.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            12656..13768
                     /gene="recF"
                     /locus_tag="O0286_000365"
     CDS             12656..13768
                     /gene="recF"
                     /locus_tag="O0286_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCY0168766.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            13778..15712
                     /gene="gyrB"
                     /locus_tag="O0286_000366"
     CDS             13778..15712
                     /gene="gyrB"
                     /locus_tag="O0286_000366"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCY0168767.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            15749..18412
                     /gene="gyrA"
                     /locus_tag="O0286_000367"
     CDS             15749..18412
                     /gene="gyrA"
                     /locus_tag="O0286_000367"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCY0168768.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(18499..19311)
                     /locus_tag="O0286_000368"
     CDS             complement(18499..19311)
                     /locus_tag="O0286_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCY0168769.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            19637..21151
                     /gene="hutH"
                     /locus_tag="O0286_000369"
     CDS             19637..21151
                     /gene="hutH"
                     /locus_tag="O0286_000369"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCY0168770.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    21224..21440
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21530..22816
                     /gene="serS"
                     /locus_tag="O0286_000370"
     CDS             21530..22816
                     /gene="serS"
                     /locus_tag="O0286_000370"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCY0168771.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     gene            23466..24161
                     /locus_tag="O0286_000371"
     CDS             23466..24161
                     /locus_tag="O0286_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCY0168772.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            24158..24487
                     /locus_tag="O0286_000372"
     CDS             24158..24487
                     /locus_tag="O0286_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCY0168773.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     regulatory      24692..24790
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            24850..25818
                     /locus_tag="O0286_000373"
     CDS             24850..25818
                     /locus_tag="O0286_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCY0168774.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     gene            26109..27047
                     /locus_tag="O0286_000374"
     CDS             26109..27047
                     /locus_tag="O0286_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCY0168775.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            27062..29029
                     /gene="gdpP"
                     /locus_tag="O0286_000375"
     CDS             27062..29029
                     /gene="gdpP"
                     /locus_tag="O0286_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCY0168776.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            29026..29472
                     /gene="rplI"
                     /locus_tag="O0286_000376"
     CDS             29026..29472
                     /gene="rplI"
                     /locus_tag="O0286_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCY0168777.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            29504..30904
                     /gene="dnaB"
                     /locus_tag="O0286_000377"
     CDS             29504..30904
                     /gene="dnaB"
                     /locus_tag="O0286_000377"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCY0168778.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            31182..32465
                     /locus_tag="O0286_000378"
     CDS             31182..32465
                     /locus_tag="O0286_000378"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCY0168779.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            32895..32969
                     /locus_tag="O0286_000379"
     tRNA            32895..32969
                     /locus_tag="O0286_000379"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32928..32930,aa:Glu,seq:ttc)
     gene            32977..33049
                     /locus_tag="O0286_000380"
     tRNA            32977..33049
                     /locus_tag="O0286_000380"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33010..33012,aa:Asp,seq:gtc)
BASE COUNT        11370 a         5492 c         6007 g        10635 t
ORIGIN      
        1 cttattattt aaaacgtcgt gaagaaaaag gcaagtaagc acattccatc ggtatagaac
       61 attgctatga cctttcaagt cagattttaa tatcaacaag tagcgtttat cgctgcttgt
      121 tttttatgga ttgcttataa aatcatcatg tttaaacgat atgtcacttt gaaaactagc
      181 aaacaccata aaagactaca atttttttat ttttataaac ccctttaaat caacgtttct
      241 aaggttttta aaaatctttt ttaatttatt ttcaaaaaac actgtacatt atgccaatat
      301 gagcgtatag ttggtcttac gtaataaaag ctcgtgaatt aaattgtagt gtatttgttt
      361 agaatatcct cttttttagt tatgaatttg ttacaaatat taagtgcaaa agcacacgga
      421 ggttttctat atgaataacg gtacagtaaa atggtttaac gcagaaaaag gttttggttt
      481 catcgaacaa gaaaatggcg gagacgtatt cgtacatttc tcaggtatcg ctagcgatgg
      541 ctacaaaact ttagaagaag gtcaaaaagt tactttcgaa atcactgaag gtcaacgtgg
      601 agaccaagca gttaacgtac aaactgttta atcttacaac ataaaacgac tcattataaa
      661 tgaatcgctt tgattaccaa caaggttcta aatcttgttg gtattttttt atgctatttt
      721 ggataatata aataatatga gacgatacca cgatgattta taaaaactta tttgccaaat
      781 acacactaag gaaaattatg aggctgagta taccaattac tgatataata gaaagcgcat
      841 ttgtcatcgt atttctagct ttagtcgttt gtacttcact aggatctttt ccattcataa
      901 aagcaacaat tcgatcgtag ttgtccattt tatgcacttt ttttaaatct tctattttca
      961 tagatgcata aaaacctatt cccaaaatga ttgccaccgt tacaccccaa gtccaattcc
     1021 aataaacaac taaaggtccc attaaaattg cagctaatgt catgaaaata agcatcacat
     1081 atgtccataa gttaagactt cgttgcgctt taatatcagg tacaaatgga atggtccctt
     1141 ttactaaatc atctaaagtt acattgaaca attcacacat cattaataag ttatgtatgt
     1201 ctggtaagct tttgtcattt tcccaattag aaatactctg cctagataca tataactttt
     1261 cagcaagata ttcttgtgaa taaccatctc gttccctata ctttttaatt tgtttactca
     1321 gattcaatta catacgcctc cagtcaatta gtacaatcat tacaatcaga ctatcagata
     1381 aagttaaatc aagtcacctt tatgattgac taaatgtttt gacatacgac aacatcgggg
     1441 ttcgaagact cccttaagac agacctgtat gattacgcgc ttgtaatttt ctcaatgcca
     1501 acttgaattt cacttattaa tgatgcaact tttccatgct attcaatgct tgcaaagtta
     1561 gtcctagtta agagattacg ttcacactat ttactattcg attttccagc tttccaacta
     1621 tgcttttcta atcttttttg tgctataaat tctaccgctt caaaaataca tactgcactg
     1681 atgaaccagc ttaggaaacc aacaacaatg ctaatcaacc acatgtgcgt catatgtaac
     1741 atcatccatg taactaaaat acaaactaat acccctgtca taccaaacac tgcgccacga
     1801 cttacatgca cagcgacttt atcaccatat tgcatacctg taataaacat agacactaaa
     1861 aatactgccg gaaacgttgc aaatataccg ccaaattctt tccaaggtag tgttacagat
     1921 acaatataac ttaataatac tgcaaatccc cccacaaaaa atttcattaa tgttaatttc
     1981 atgatgttct gcctccaata atttattact taataactga atacaactaa ttatcacgca
     2041 ttttccaaac tataaatgtc taatactgaa tattgcgagg gaaatgacaa accaacaaac
     2101 aactgtgaat attgcgcctt tccgataacc tttatgctta atgtagagag atgtcaaaaa
     2161 tacagttaat atacaagaca gaataccgac aattgctcca gtactaagat tcatcgacat
     2221 ctccactaat tgcgtaccac gatgatctaa agctaatgcg ataatagccg caagaaagac
     2281 tgccggcata gtagctataa tacctcctaa cttaccacct accttatcag caataattga
     2341 agctaatgca acagcgatac caccaatgac aaaatgaaga attgcacttc caattgaaaa
     2401 catcttcaac gtacctttct ataattaatt caatacaaaa cgtatcattc acacaaagtg
     2461 taaaacttaa attttctact gtaaaggcaa ctcaagcgat gttcataaag ctttcatgat
     2521 actttcataa actagcaata aagggttcaa atgaaagtat atccagctca aattagggag
     2581 tgggacagaa atgatatttt cgcaaaattt atttcgtcgt cccaccccag caaggttgac
     2641 tagaattgaa aaaagcttgt tacaagcgca ttttcgttca gtcaactact gccaatataa
     2701 cttcgtagag catagaacat tgatttatgt accagcctga tcaacatata aatataaatt
     2761 tttatgtttc acgtaaaaca aaaaaaaaga caaagctgtt atgatcttag ctttgtcttt
     2821 tatgtgttag tcatcatgta tacgacatcc tactactaac gtttatatat tcgaattttt
     2881 atttcataat aatcttcatg atctttgtct ttatgctcaa catgtaatcc tgtttgttga
     2941 atcgcttgga tacttttgcc tacctcatct cgtgcttgag tgacatcttg tgcaaagcga
     3001 aggttttgtg ctttgacttt ttcgggcccc gttttttggc gtactctatc ttcagtctgt
     3061 ttcacattta gcttttgtgc aatgacttgc tcaatcaacg cttcttgttc gctatcagac
     3121 aatgataata ccgctctcgc atgacgttca gtaattttac cttctcttaa gcgaagtagt
     3181 actttcggcg ccaacttcaa taaacgcaac ttatttgcaa taaagctttg acttttacct
     3241 aaactttttg ccaattcact ttgcgttgta tcaccaattt ccaataattt cttataggct
     3301 tccgcttctt caacaacaga caaattttct ctttgaatat tctcaattaa tgcaacaaca
     3361 gccgtctctt catcatccat atcacgaata ataacgtctg cttgaggtaa atttagtgat
     3421 tgtattgctc taaatcggcg ctctccagca ataatttcaa acatatcttc ttcaatcggt
     3481 cttacaacaa taggttgtag taaaccatgt tcatgtattg attcagcaag ttctttaatt
     3541 ttatttggtt caaacacctg tcttggttga taacggttgg gaacgatacg ttcaatttga
     3601 atggattcaa cattactatt gcgatcttct tcaatatgtc caatgatgtc atctttgttt
     3661 ttcaaaccaa ataattttga aaaaggtttt ttcattatcc attcgctcct tttatgttag
     3721 ttgtaaagga ttatgcatta tttttcaagt aaaggagtct tattaggcgt ccctggtttt
     3781 cttggatact ttttcggcgt ctgtcttttt ttatcaataa tgaacatctg gcgctctcca
     3841 gcatcttctg gcaattcaaa ggtatgtgtt tctgtaacat taccacctaa cacactaatt
     3901 gcaaattttg cttcttctaa ttcttcttca ccttttgaag attttaatgc aacaaactgt
     3961 ccaccttttt taactagcgg taaacacaat tcacttaaca cggataatct agctactgct
     4021 cttgcagtaa caacatcata agactccctg tagacaccct taccaaatgt ttctgctcta
     4081 tcgtgtataa agctgacatc ctgtaattgt aattctgacg ctaaatggtt taaaaattga
     4141 atacgcttat ttaatgaatc aacaatcgtc acttttaact gcggaaacat tatttttaac
     4201 ggaatacttg gaaaaccagc tccagcgcct acatcacata tacttatagg ctgattaaaa
     4261 tcaaaataaa aactaggtgc aatggaatca taaaaatgtt tcaaatatac atcgtgttca
     4321 tctgtaatac ttgtcaaatt catcttttca ttccattcaa caagtaaacg ataatatgtt
     4381 tgaaactgtt gtttttgagt ctcagttaat tgaatattat gttcttttaa ttgttctgct
     4441 aaccattcta cagtcattag tcactcaccc tttggagttt accttgttct aagtaaatca
     4501 ataatataga tatgtctgct ggatttaccc ctgatattct agaagcttgt gcaatattta
     4561 aaggttttac ttctgataat ttttctcgcg cttcagtcgc caaactatca atcttactat
     4621 aatctaagtc ttctggaatt ttcttctctt ccatacgctt aactttctca acttgttgta
     4681 gtgatttatt gatataacct tcatattttg tttgtatttc tacttgttct tcaacatctg
     4741 cattcaattg atgttcttct tctaaaagtt ctaaaattat atcgtaagtc atttcaggtc
     4801 tgcgtaataa atcgatagct aaaataccat cttttaagcg agaaccacca tgttgttcaa
     4861 taatcgcttg cgtatgttcg tttggtttaa tacgaatatc tgataaacgc ttaatttccg
     4921 catcaatttg ctgacgtttt tcattaaaac gtgcatatct ttcttcagaa atcataccaa
     4981 gttcatatcc catatccgtc aatctcaaat cagcattatc atgacgtagt aacaaacgat
     5041 attctgcacg tgatgttagt aaacgataag gttcattagt acctttagtt acaagatcat
     5101 cgattaagac accaatatat gcatctgaac gacttaatat cttttcgcct gtgtttaaca
     5161 ctttacctgc agcgttaata cctgccatca atccttgtcc tgctgcttct tcataaccag
     5221 atgtaccatt aatttgacct gcagtatata agtttttaat cattttcgtt tcaagtgtag
     5281 gccataactg cgttggcaca atcgcatcat attcaattgc gtagccggca cgcatcatat
     5341 ctgctttttc aagacctggt atcgtctcta acatttgacg ttgcacatgt tcaggaagac
     5401 ttgtagacaa tccttgcaca tatacttcat ttgtattacg accttcaggc tctaagaaaa
     5461 gttgatgtcg cggcttatca ttaaatcgaa caaatttatc ttcaattgaa gggcaataac
     5521 gtggcccggt tcctttaatc atccctgaat acattgcaga tagatgtaaa ttatcatcga
     5581 taactttgtg tgtttcagca ttagtatacg ttagccaaca tggcaattga tctaatatat
     5641 attctgttgt ttcaaagctg aatgcacgac ctacatcgtc acctggttgt atttcagtct
     5701 tcgaatagtc aattgttttt gaatttacac gcggtggtgt acctgtttta aaacgaacaa
     5761 tatcaaaacc aagttctctt aaattgtctg ataatgtgat tgatggtaat tggtgatttg
     5821 gtccacttga atacttcata ttacctaaaa tgatttcacc acgtaaaaat gttcccgttg
     5881 taataattac tgctttagat aaatactctg taccaatatt tgtacgtaca cctttaactt
     5941 cattatcttc tataataagt tcgtctacca taccttgcat tatatgcaaa ttttcttcat
     6001 cttcaatcac gcgtttcatt tcttgttgat aaagtacttt atctgcttgc gctcttagtg
     6061 ctcttacagc aggtccttta cctgtattta acattctcat ttgaatgtgt gttttatcga
     6121 ttgtttttgc catttgtcca cctaaagcat caatttcacg aacaacgata cctttagctg
     6181 gtccacctac agatgggtta catggcataa atgcaatatt atctaaattt attgttagca
     6241 ttaatgtttt agcaccacgt cttgcagatg ctaaacctgc ttctacacct gcatgtcccg
     6301 cacctataac gattacatca tattcttgaa ccacaatata aacctcctta tttgatatct
     6361 tactagcctt cttaagacgg tattccgtct atttcaatta ctatttacct aagcagaatt
     6421 gactgaataa ctgatcgatg agttcatcac ttgcagtctc accaataatt tctcctaata
     6481 tttcccaagt tctagttaaa tcaatttgta ccatatccat aggcacacca gattctgctg
     6541 catcaatcgc atcttgtatc gtttgtcttg cttgttttaa taatgaaata tgtcttgaat
     6601 tagaaacata agtcatatct tgattttgta cttctccacc aaagaacaaa tctcgaattt
     6661 gtatttctaa ttcatcaata ccttcttgtt ttaacattga agtttgaatt aatggcgtat
     6721 cacctatcat atctttaact tcattaatat ctatgttttg ctctaaatcc attttattaa
     6781 caattacgat tacatcttca tttttaacca cttcatataa tgtgtaatct tcttgagtca
     6841 atgcttcgtt attgtttaat acaaataaaa ttaagtctgc ttggctaaga gcctttctag
     6901 agcgttcaac accaatcttc tctactatat cttctgtctc acgtatacca gcagtatcaa
     6961 ctaatcttaa tggcacgcca cgaacattga cgtactcttc taagacatct ctagtagtac
     7021 ctgctacctc agttacaatc gctttattat cttgtattaa attatttaac atcgatgatt
     7081 tacctacgtt tggtttacca acaataactg tagataaacc ttcacgcata attttaccct
     7141 gcgcaccggt atctaataaa cgattaattt cctgtttgat ttctttagac tgctctaaaa
     7201 gaaattcagt agtcgcatct tcaacatcat cgtattcagg ataatcaata ttcacttcca
     7261 cttgagcgag tatctctaat atagattgac gttgtttttt gattaagtca cttagacgac
     7321 cttcaatttg attcatcgca actttagaag ctctatctgt cttcgagcga ataaagtcca
     7381 taactgcttc agcttgagat aaatcaatac gaccatttaa aaaggcacgt tttgtaaatt
     7441 cacctggctc agccattcta gcgccatatg tcatagtaag ttccagcact ctattaatcg
     7501 ttaaaatacc accatgacaa ttaatttcta taatatcttc gcgtgtaaat gtttttggcg
     7561 ctcttaacac agacaccata acttcttcaa tcacttcttt agactctgga tcaataatat
     7621 gaccgtaatt aatcgtatgt gatggaacat catttaaaag atgttttcct ttatataatt
     7681 tgtcagcaat ttcaacggct tgcggtccag acaatcgaac aattccaatt gccccttcac
     7741 ccattggtgt tgaaatactc gtaattgtat ctaaatccat attgctactc gcctccttca
     7801 acgatgtgaa tacattttaa agtaagttat tataacccta aggtcagtct tacgtttgtc
     7861 tgaggtaaga cttcgggatg tgttgagtgg ttaatgtttt ccttccccta ccctatcctt
     7921 acttaatctt tttattaaaa actttggcaa ttttaagtac gtgctcaaga ctattctgta
     7981 tttgtaaagt cgtcatatct ttagctggct gtcttgctat tacaataata tctttggcca
     8041 atatatgcga cttatgtact ttgaaatttt cacgtattgc tcttttaatc ttgtttctta
     8101 acactgcatt acctagtttt ttagaaacac taatacctaa gcgaaaatgg tctatttctt
     8161 tattattaca agtgtataca acaaattgtc tgttggctac agaatgacct tttttatata
     8221 ttctctgaaa atctgcattc tttttaattc ggtaagcttt ttccaataac atcactcgct
     8281 tatttatcgt ttttatttga agctatattt aaacttctat tgagcttata acataaattt
     8341 ctatttattc ttaatttaaa cgaaaaaaaa gatcactgat gagtcagtga tcttatgcag
     8401 ataaaacttt acggccttta cgacgacggc gcgctaaaac tttacggcca ttttttgtgc
     8461 tcatgcgttt tctgaaacca tgaactttac tatgtttacg tttatttggt tgataagtac
     8521 gttttaccat gcaaaacacc tccatcttta tacagttatc taatatacca gattatgcag
     8581 actctgatca ctttaataga taagattttg cgtttcttag attaaaattc tcgtattagc
     8641 tcattgatta tctagtcata attcaagcaa ctactacaat ataacaaaat cctatttata
     8701 acgcaagttc attttatact actgctcaat ttttttactt ttatcgatta aagatagaaa
     8761 tacacgatgc gagcaatcaa atttcataac atcaccatga gtttggtccg aagcatgagt
     8821 gtttacaatg ttcgaacacc ttatacagtt cttatacata ctttataaat tatttcccaa
     8881 actgttttga tacactcact aacagatact ctatagaagg aaaagttatc cacttatgca
     8941 catttatagt tttcagaatt gtggataatt agaaattaca cacaaagtta tactattttt
     9001 agcaacatat tcacaggtat ttgacatata gaggactgaa aaagtataat tgtgtggata
     9061 agtcgtccaa ctcatgattt tataaggatt tatttattga tttttacata aaaatactgt
     9121 gcataactaa taagcaagat aaagttatcc accgattgtt attaacttgt ggataattat
     9181 taacatggtg tgtttagaag ttatccacgg ctgttatttt tgtgtataac ttaaaaattt
     9241 aagaaagatg gagtaaattt atgtcggaaa aagaaatttg ggaaaaagtg cttgaaattg
     9301 ctcaagaaaa attatcagct gtaagttact caactttcct aaaagatact gagctttaca
     9361 cgattaaaga tggtgaagct atcgtattat cgagtattcc ttttaatgca aattggttaa
     9421 atcaacaata tgctgaaatt atccaagcaa tcttatttga tgttgtaggc tatgaagtta
     9481 aacctcactt tattactact gaagaattag caaattatag taataatgaa actgctactc
     9541 caaaagaaac aacaaaacct tctactgaaa caactgagga taatcatgtg cttggtagag
     9601 agcaattcaa tgcccataac acatttgaca cttttgtaat cggacccggt aaccgctttc
     9661 cacatgcagc gagtttagct gtggccgaag caccagccaa agcgtacaat ccattattta
     9721 tctatggagg tgttggttta ggaaaaaccc atttaatgca tgccattggt catcatgttt
     9781 tagataataa tccagatgcc aaagtgattt acacatcaag tgaaaaattc acaaatgaat
     9841 ttattaaatc aattcgtgat aacgaaggtg aagctttcag agaaagatat cgtaatatcg
     9901 acgtcttatt aatcgatgat attcagttca tacaaaacaa ggtacaaaca caagaagaat
     9961 ttttctatac ttttaatgaa ttgcatcaga ataacaagca aatagttatt tcgagtgatc
    10021 gaccaccaaa ggaaattgca caattagaag accgattacg ttcacgcttt gaatgggggc
    10081 taattgttga tattacgcca ccagattatg aaactcgaat ggcaattttg cagaagaaaa
    10141 ttgaagaaga aaaattagat attccaccag aagctttaaa ttatatagca aatcaaattc
    10201 aatctaatat tcgtgaatta gaaggtgcat taacacgttt acttgcatat tcacaattat
    10261 taggaaaacc aattacaact gaattaactg ctgaagcttt aaaagatatc attcaagcac
    10321 caaaatctaa aaagattacc atccaagata ttcaaaaaat tgtaggccag tactataatg
    10381 ttagaattga agatttcagt gcaaaaaaac gtacaaagtc aattgcatat ccgcgtcaaa
    10441 tagctatgta cttgtctaga gagcttacag atttctcatt acctaaaatt ggtgaagaat
    10501 ttggtgggcg tgatcatacg accgtcattc atgctcatga aaaaatatct aaagatttaa
    10561 aagaagatcc tatttttaaa caagaagtag agaatcttga aaaagaaata agaaatgtat
    10621 aagtaggaaa ctttgggaaa tgtaatctgt tatataacag cactaatgat aacaatcatt
    10681 ttttacattt ctatatgcta atgtggcaag atgagcaaaa ctcattttgt ggataatgtt
    10741 taaaagtcat acacaccata cacaagttat caacatgtgt ataacttcgc caaatctatg
    10801 tttttaagac ttatccacca atccacagca cctactacta ttactaagaa cttaaaacct
    10861 atataattat atataaacga ctggaaggag ttttaattaa tgatggaatt cactattaaa
    10921 agagattatt ttattacaca attaaatgac acattaaaag ctatttcacc aagaacaaca
    10981 ttacctatat taactggtat caaaatcgat gcgaaagaac atgaagttat attaactggt
    11041 tcagactctg aaatttcaat agaaatcact attcctaaaa ctgtagatgg cgaagatatt
    11101 gtcaatattt cagaaacagg ctcagtagta cttcctggac gattctttgt tgatattata
    11161 aaaaaattac ctggtaaaga tgttaaatta tctacaaatg aacaattcca gacattaatt
    11221 acatcaggtc attctgaatt taatttaagt ggcttagatc cagatcaata tcctttatta
    11281 cctcaagttt ctagagatga cgcaattcaa ttgtcggtaa aagtgcttaa aaacgtgatt
    11341 gcacaaacaa attttgcagt gtccacctca gaaacacgcc cagtactaac tggtgtgaac
    11401 tggcttatac aagaaaatga attaatatgc acagcgactg actcacaccg cttggctgta
    11461 agaaagttgc agttagaaga tgtttctgaa aacaaaaatg tcatcattcc aggtaaggct
    11521 ttagctgaat taaataaaat tatgtctgac aatgaagaag acattgatat cttctttgct
    11581 tcaaaccaag ttttatttaa agttggaaat gtgaacttta tttctcgatt attagaagga
    11641 cattatcctg atacaacacg tttattccct gaaaactatg aaattaaatt aagtatagac
    11701 aatggggagt tttatcatgc gattgatcgt gcctctttat tagcgcgtga aggtggtaat
    11761 aacgttatta aattaagtac aggtgatgac gttgttgaat tgtcttctac atcaccagaa
    11821 attggtactg taaaagaaga agttgatgca aacgatgttg aaggtggtag cctgaaaatt
    11881 tcattcaact ctaaatatat gatggatgct ttaaaagcaa tcgataatga tgaggttgaa
    11941 gttgaattct tcggtacaat gaaaccattt attctaaaac caaaaggtga cgactcggta
    12001 acgcaattaa ttttaccaat cagaacttac taaaaataaa tataaataaa ggatgacgtg
    12061 attaattaaa acgtcatcct ttattttttg gcaaaaataa ttctaggtgc gtatgtaaaa
    12121 taaatttggc agcattttaa acagcaaata aaagacgcca attaaattta tgacaaatgt
    12181 atccaaaatt taataagtgt gcttatatgc cctttaaatt taaaatttta atagtcaata
    12241 acaagttgaa tataaaagtt aaacgccgtt aaatagcgtt aaaaaattga aaatgacagt
    12301 attgccaaaa aataagaatt aattatttat atgtaaacgg tttctacctc tattttaaat
    12361 gaaatttgtg acaaaaaaag gtataatata ttaatgacat acaaagaaat ggagtgatta
    12421 ttttggttca agaagttgta gtagaaggag acattaattt aggtcaattt ctaaaaacag
    12481 aagggattat tgaatctggt ggtcaagcaa aatggttctt gcaagacgtt gaagtattaa
    12541 ttaatggagt gcgtgaaaca cgtcgcggta aaaagttaga acatcaagat cgtatagata
    12601 tcccagaatt acctgaagat gctggttctt tcttaatcat tcatcaaggt gaacaatgaa
    12661 gttaaataca ctccaattag aaaattatcg taactatgat gaggttacgt tgaaatgtca
    12721 tcctgacgtg aatatcctca ttggagaaaa tgcacaagga aagacaaatt tacttgaatc
    12781 aatttatacc ttagctttag caaaaagtca tagaacgagt aatgataagg aactcatacg
    12841 ttttaatgct gattatgcta aaatagaagg tgagcttagt tatagacacg gcacgatgcc
    12901 attaacaatg tttataacta aaaaaggtaa acaagtcaaa gtgaatcact tagagcaaag
    12961 tcgtctaact caatatattg gacacctcaa tgtggttcta tttgcgccag aagatttgaa
    13021 tattgtaaaa ggctctcctc aaataagacg acgctttata gatatggagt tgggccaaat
    13081 ttctgctgtt tacttaaatg atttagctca ataccaacgt attttaaagc aaaagaataa
    13141 ttacttaaag cagttacaat taggccaaaa aaaggactta acaatgttgg aagtattaaa
    13201 tcagcagttt gctgaatatg caatgaaagt aactgataaa cgtgcacatt ttattcaaga
    13261 gctagagtcg ttagctaaac cgattcatgc tggtatcaca aatgataaag aagcgttgtc
    13321 gctgaattat ttacctagtc ttaaatttga ttatgctcaa aatgaagcgg cacgacttga
    13381 agaaattatg tctattctta gcgataatat gcaaagagaa aaagaacgag gcattagctt
    13441 attcggacca catcgagatg atataagttt tgatgtgaat ggcatggatg ctcaaacata
    13501 tggttctcaa ggacagcaac gtacaacggc tttgtccatt aaattagctg aaattgagtt
    13561 aatgaatatc gaagttgggg aatatcccat cttattatta gacgatgtac tcagtgaatt
    13621 agatgattcg cgtcaaacgc atttattaag tacgattcag cataaagtac aaacatttgt
    13681 cactacgaca tctgtagatg gtattgatca tgaaatcatg aataacgcta aattgtatcg
    13741 tattaatcaa ggtgaaatta taaagtaaca gaaagcgatg gtgactgcat tgtcagatgt
    13801 aaacaacacg gataattatg gtgctgggca aatacaagta ttagaaggtt tagaagcagt
    13861 acgtaaaaga ccaggtatgt atataggatc gacttcagag agaggtttgc accatttagt
    13921 gtgggaaatt gtcgataata gtatcgatga agcattagct ggttatgcaa atcaaattga
    13981 agttgttatt gaaaaagata actggattaa agtaacggat aacggacgtg gtatcccagt
    14041 tgatattcaa gaaaaaatgg gacgtccagc tgtcgaagtt attttaactg ttttacatgc
    14101 tggtggtaaa tttggcggtg gcggatacaa agtatctggt ggtttacatg gtgttggttc
    14161 atcagttgta aacgcattgt cacaagactt agaagtatat gtacacagaa atgagactat
    14221 atatcatcaa gcatataaaa aaggtgtacc tcaatttgac ttaaaagaag ttggcacaac
    14281 tgataagaca ggtactgtca ttcgttttaa agcagatgga gaaatcttca cagagacaac
    14341 tgtatacaac tatgaaacat tacagcagcg tattagagag cttgctttct taaacaaagg
    14401 aattcaaatc acattaagag atgaacgtga tgaagaaaac gttagagaag actcctatca
    14461 ctatgagggc ggtattaaat cgtacgttga gttattgaac gaaaataaag aacctattca
    14521 tgatgagcca atttatattc atcaatctaa agatgatatt gaagtagaaa ttgcgattca
    14581 atataactca ggatatgcca caaatctttt aacttacgca aataacattc atacgtatga
    14641 aggtggtacg catgaagacg gattcaaacg tgcattaacg cgtgtcttaa atagttatgg
    14701 tttaagtagc aagattatga aagaagaaaa agatagactt tctggtgaag atacacgtga
    14761 aggtatgaca gcaattatat ctatcaaaca tggtgatcct caattcgaag gtcaaacgaa
    14821 gacaaaatta ggtaattctg aagtgcgtca agttgtagat aaattattct cagagcactt
    14881 tgaacgattt ttatatgaaa atccacaagt cgcacgtaca gtggttgaaa aaggtattat
    14941 ggcggcacgt gcacgtgttg ctgcgaaaaa agcgcgtgaa gtaacacgtc gtaaatcagc
    15001 gttagatgta gcaagccttc caggtaaatt agccgattgc tctagtaaaa gtcctgaaga
    15061 atgtgagatt ttcttagtcg aaggggactc tgccgggggg tctacaaaat ctggtcgtga
    15121 ctctagaacg caggcgattt taccattacg aggtaagata ttaaatgttg aaaaagcacg
    15181 attagataga attttgaata acaatgaaat tcgtcaaatg atcacagcat ttggtacagg
    15241 aatcggtggc gactttgatc tagcgaaagc aagatatcac aaaatcgtca ttatgactga
    15301 tgccgatgtg gatggagcgc atattagaac attgttatta acattcttct atcgatttat
    15361 gagaccgtta attgaagcag gctatgtgta tattgcacag ccaccgttgt ataaactgac
    15421 acaaggtaaa caaaagtatt atgtatacaa tgatagggaa cttgataaac ttaaatctga
    15481 attgaatcca acaccaaaat ggtctattgc acgatacaaa ggtcttggag aaatgaatgc
    15541 agatcaatta tgggaaacaa caatgaaccc tgagcaccgc gctcttttac aagtaaaact
    15601 tgaagatgcg attgaagcgg accaaacatt tgaaatgtta atgggtgacg ttgtagaaaa
    15661 ccgtagacaa tttatagaag ataatgcagt ttatgcaaac ttagacttct aagcgctgtg
    15721 aactgaactt ttgaaggagg aactcttgat ggctgaatta cctcaatcaa gaataaatga
    15781 acgaaatatt accagtgaaa tgcgtgaatc atttttagat tatgcgatga gtgttatcgt
    15841 tgctcgtgca ttgccagatg ttcgtgacgg tttaaaacca gtacatcgtc gtatactata
    15901 tggattaaat gaacaaggta tgacaccgga taaatcatat aaaaaatcag cacgtatcgt
    15961 tggtgacgta atgggtaaat atcaccctca tggtgactta tctatttatg aagcaatggt
    16021 acgtatggct caagatttca gttatcgtta tccgcttgtt gatggccaag gtaactttgg
    16081 ttcaatggat ggagatggcg cagcagcaat gcgttatact gaagcgcgta tgactaaaat
    16141 cacacttgaa ctgttacgtg atattaataa agatacaata gattttatcg ataactatga
    16201 tggtaatgaa agagagccgt cagtcttacc tgctcgattc cctaacttat tagccaatgg
    16261 tgcatcaggt atcgcggtag gtatggcaac gaatattcca ccacataact taacagaatt
    16321 aatcaatggt gtacttagct taagtaagaa ccctgatatt tcaattgctg agttaatgga
    16381 ggatattgaa ggtcctgatt tcccaactgc tggacttatt ttaggtaaga gtggtattag
    16441 acgtgcatat gaaacaggtc gtggttcaat tcaaatgcgt tctcgtgcag ttattgaaga
    16501 acgtggaggc ggacgtcaac gtattgttgt cactgaaatt cctttccaag tgaataaggc
    16561 tcgtatgatt gaaaaaattg cagagctcgt tcgtgacaag aaaattgacg gtatcactga
    16621 tttacgtgat gaaacaagtt tacgtactgg tgtgcgtgtc gttattgatg tgcgtaagga
    16681 tgcaaatgct agtgtcattt taaataactt atacaaacaa acacctcttc aaacatcatt
    16741 tggtgtgaat atgattgcac ttgtaaatgg tagaccgaag cttattaatt taaaagaagc
    16801 gttggtacat tatttagagc atcaaaagac agttgttaga agacgtacgc aatacaactt
    16861 acgtaaagct aaagatcgtg cccacatttt agaaggatta cgtatcgcac ttgaccatat
    16921 cgatgaaatt atttcaacga ttcgtgagtc agatacagat aaagttgcaa tggaaagctt
    16981 gcaacaacgc ttcaaacttt ctgaaaaaca agctcaagct attttagaca tgcgtttaag
    17041 acgtctaaca ggtttagaga gagacaaaat tgaagctgaa tataatgagt tattaaatta
    17101 tattagtgaa ttagaagcaa tcttagctga tgaagaagtg ttattacagt tagttagaga
    17161 tgaattgact gaaattagag atcgtttcgg tgatgatcgt cgtacagaaa ttcaattagg
    17221 tggatttgaa aacttagagg acgaagactt aattccagaa gaacaaatag taattacact
    17281 aagccataat aactacatta aacgtttgcc ggtatctaca tatcgtgctc aaaaccgtgg
    17341 tggtcgtggt gttcaaggta tgaatacatt ggaagaagat tttgtcagtc aattggtaac
    17401 tttaagtaca catgaccatg tattgttctt tactaacaaa ggtcgtgtat acaaacttaa
    17461 aggttacgaa gtgcctgagt tatcaagaca gtctaaaggt attcctgtag tgaatgctat
    17521 tgaacttgaa aatgatgaag tcattagtac aatgattgct gttaaagacc ttgaaagtga
    17581 agacaacttc ttagtgtttg caactaaacg tggtgtcgtt aaacgttcag cattaagtaa
    17641 cttctcaaga ataaatagaa atggtaagat tgcgatttcg ttcagagaag atgatgagtt
    17701 aattgcagtt cgcttaacaa gtggtcaaga agatatcttg attggtacat cacatgcatc
    17761 attaattcga ttccctgaat caacattacg tcctttaggc cgtacagcaa cgggtgtgaa
    17821 aggtattaca cttcgtgaag gtgacgaagt tgtagggctt gatgtagctc atgcaaacag
    17881 tgttgatgaa gtattagtag ttactgaaaa tggttatggt aaacgtacgc cagttaatga
    17941 ctatcgctta tcaaatcgtg gtggtaaagg tattaaaaca gctacgatta ctgagcgtaa
    18001 tggtaatgtt gtatgtatca ctacagtaac tggtgaagaa gatttaatga ttgttactaa
    18061 tgcaggtgtc attattcgac tagatgttgc agatatttct caaaatggtc gtgcagcaca
    18121 aggtgttcgc ttaattcgct taggtgatga tcaatttgtt tcaacggttg ctaaagtaaa
    18181 agaagatgca gaagatgaaa cgaatgaaga tgagcaatct acttcaactg tatctgaaga
    18241 tggtactgaa caacaacgtg aagcggttgt aaatgatgaa acaccaggaa atgcaattca
    18301 tactgaagtg attgattcag aagaaaatga tgaagatgga cgtattgaag taagacaaga
    18361 tttcatggat cgtgttgaag aagatataca acaatcatca gatgaagaat aataaaaaat
    18421 aagacttccc tatatgtagg ggagtcttat ttttatgcta gaaagtaatg ctttactata
    18481 ttcaatgatt agtaatgact aactttctaa ttgtttcatt gcgtaaggta tttcattgat
    18541 aagtcttgat ggtggcacca catacatatc ttttgcaagg ttttcgccaa taaaactatg
    18601 tgtatatgtg gcactcataa ccgcttcttt taagttatca aattgaccga caaagcttgt
    18661 aatcatacca gcaagtgtat cgcccatacc accagtcgcc attgctgggc taccgattgt
    18721 caatttaaag tcttcatctt taaagaaaat ttcagtacca tgttttttaa gtacaacagt
    18781 tgcacctaaa cgatcaactg cttcacgatt acgctcatat gtctgttcct caataggaat
    18841 accacttaat cgttcccatt ctttgaggtg tggtgtaaag atcacacgac atgtaggtaa
    18901 ttgcggtttc agtttactaa agattgtaat cgcatcgccg tctacgatta aattttgatg
    18961 cggttgtata ttttgtagta ggaatgtaat ggcattattt cctttgaaat caacgccaag
    19021 acctggacca attagtatac tgtcagtcat ttcaatcatt ttcgtcaaca ttttcgtatc
    19081 attaatatca ataaccatcg cttctgggca acgagaatgt aatgctgaat gatttgttgg
    19141 atgtgtagct acagtgatta aaccactacc gctaaataca catgcacgag ccgctaacat
    19201 aatggcacca cctaagttag cagatccacc aattaataaa attttgccat aatcaccttt
    19261 atgtgaatct tctttacgct taggaatgtt aatagaattt aacgtttcca tagtgatata
    19321 acctcccatg taaaagcctt ttccgaattt attcaatttt aaaaatatat agtaactttt
    19381 aacaaaatgt attataaatt tctgaattca ttattatttg tcgttaaata caatagaaaa
    19441 tactatacct gtatatgcaa ttcgtcaata gataaattat taaatatgct tacaacaatc
    19501 ttaatatcct ttaacgcact acaatagtgc tctgataata ggttataaat gtacgtaaaa
    19561 ccattgtttc aataaaaatg aaaacgtata cttcaagaag gatgggttac ttaatataaa
    19621 caagggggta acatatatga ctttatattt agatggtgaa acactaacaa ttgaggatat
    19681 taaatcattt ttacaacaac aatcaaagat tgaaattatt gatgatgcgt tagaacgtgt
    19741 caaaaaaagt agagcggtag ttgaacgtat tattgaaaat gaggaaacgg tttacggtat
    19801 tactacaggt tttgggttat ttagtgatgt acgtatagac ccgacgcaat ataatgaatt
    19861 acaagtgaat ctgatacgct cacatgcctg tggactaggt gagccatttt caaaagaagt
    19921 agcattagtc atgatgattt tacgattgaa tacattatta aaaggtcatt caggtgccac
    19981 tttagaatta gtgagacaat tacaattttt tataaatgaa cgtattatac cgataatccc
    20041 acagcaaggc tctctcggtg catcaggaga tttagcgcca ttatcacatt tagcattagc
    20101 attaattggt gaagggaaag tattgtacag aggggaagaa aaggatagtg acgatgtatt
    20161 aagagaatta aatagacaac ctttgaacct tcaggctaaa gaaggtttag cattgattaa
    20221 tggtacgcaa gctatgacag ctcaaggtgt cattagttat atagaagcag aagatttagg
    20281 ttaccaatct gaatggattg ctgcattaac gcatcagtct cttaatggca ttatagatgc
    20341 atatcgacat gatgtgcacg cagttcgtaa ttttcaagaa cagattaatg tggcagcgcg
    20401 tatgcgtgat tggttagaag gatcaacatt aacgacgcga caatcagaaa tacgtgtaca
    20461 agatgcatat acgttgcgtt gtataccaca aatccatggc gcgagttttc aagtattcaa
    20521 ttatgttaaa cagcaattag aatttgaaat gaatgcggct aatgataatc cacttatatt
    20581 tgaggaagca aatgaaacgt ttgttatttc aggtggtaac ttccatggac aacctattgc
    20641 ttttgcatta gatcatctta aattaggtgt aagtgaatta gcaaacgtat cggaacgtcg
    20701 tctagagcga ctagtaaatc ctcaattaaa tggtgattta ccagcatttc ttagtccaga
    20761 gccaggattg caaagtggcg cgatgattat gcaatatgct gctgcaagtc tcgtttctga
    20821 aaataaaact ttagcgcatc cagcgagtgt tgattctatc acttcatctg cgaaccaaga
    20881 agatcacgta tctatgggaa ctacagctgc tagacatggt tatcaaatta ttgaaaatgc
    20941 aagacgtgtg ctggcaatcg aatgtgttat tgcattacaa gcagcagagt taaaaggtgt
    21001 tgaaggatta tcaccaaaaa cacgtcgcaa gtatgatgag tttcgaagta tcgtgccatc
    21061 cattacacat gatcgtcaat ttcataaaga tattgaagcg gttgcacagt atttaaagca
    21121 atcaatttat caaacgactg catgtcacta aatcgacatg agtgatgttt gaaatcgtta
    21181 cttgaaaaag caaatatagt ttgctatatt aaaaataact taataagaca ttgttcgtag
    21241 gacaagtaat atatagtgtt cgatatcaga gagcttgtgg ttagtgtgaa caagaatcaa
    21301 catatatatg aatctaccta cttaatttaa aagaacaatc ggtgataacc gttattttag
    21361 tgaagtgcaa tttaggttta gtgtatcttt ataacttaaa ttgttaaata gggtggcaac
    21421 gcgtagacca cgtcccttgt agggatgtgg tcttttttta ttttctaaaa aataaagacg
    21481 tacgtcattc cataataaat gataattatt tgggaaagga tgaaggttaa tgttagacat
    21541 tagattattc agaaatgagc ctgacacagt taagagcaaa attgaattac gtggagatga
    21601 tccaaaagtt gtagatgaaa ttttagaatt ggatgagcaa cgacgtaaat taattagtgc
    21661 aacagaagaa atgaaagcac gtcgtaataa agtaagcgaa gaaatcgcat taaaaaaacg
    21721 taataaagaa aatgctgatg atgtgattgc tgaaatgcgc acattaggtg acgatattaa
    21781 agaaaaagat agtcaattaa atgaaattga taataaaatg acaggtatcc tttgtcgtat
    21841 tccaaattta ataagtgatg atgtacctca aggtgaatct gatgaagata acgttgaagt
    21901 taaaaagtgg ggtacaccac gtgagttttc atttgaaccc aaagcacatt gggatattgt
    21961 agaagaattg aaaatggctg attttgatcg tgcagcaaaa gtttcaggtg cgcgttttgt
    22021 atatttaaca aatgaaggtg cgcaattaga gcgtgcttta atgaactata tgattacaaa
    22081 acatacaaca caacatggtt atacagaaat gatggtacca cagcttgtga acgcagatac
    22141 aatgtatggt acaggtcaat tacctaaatt tgaagaagat ttatttaaag tagaaaaaga
    22201 aggattatat acaattccaa ctgctgaagt accattaacg aatttctacc gtaatgaaat
    22261 tattcaacca ggtgtacttc ctgaaaaatt cactggtcaa tctgcatgtt tccgtagtga
    22321 agcaggatca gcaggtagag atacaagagg attaattcgt ttacatcaat tcgataaagt
    22381 ggaaatggta cgttttgaac aacctgaaga ttcatggaat gctttagaag aaatgacaac
    22441 aaacgcagaa gcaattctag aagagttagg tttaccatac cgtcgtgtta ttttatgtac
    22501 aggtgatatt ggatttagtg caagcaaaac atatgattta gaagtttggt taccaagcta
    22561 caatgattat aaagaaatta gttcatgctc aaactgtacg gatttccaag cgcgtcgtgc
    22621 taacatccgc ttcaagcgtg acaaagcagc taaaccagaa ttagcacata cattaaatgg
    22681 tagtggttta gcagttggac gtacatttgc tgctattgtt gaaaattacc aaaatgaaga
    22741 tggaacagta acaattccag aagcattagt accatttatg ggtggtaaaa cacaaatttc
    22801 aaaaccagtt aaataaaggc tttagctaca agctttaaaa agtatatatc tacgtatact
    22861 taaagcaagg gcaagatact ttaaataata ttttaaaaag tggtgacgaa gctgtcgcca
    22921 ctttttttgt gctgtaaaag tataatagtg agccaatgca cataacaaca ataaattaag
    22981 tttgtggttt aatggggtga acgcatttca ttatagcaac aatacgggat aattatgatg
    23041 aactaaaaca atctaaaacg taacaagttt gagcatcacc aatataggaa aggaagcgat
    23101 aaaatactga tttcgttgat atgtagtatg agttatatcg atggagtagg gtagggggag
    23161 gggatgatta taagggagtg gtacatgaat caatatccca gactcatcat cagatataaa
    23221 aatttataaa attgatactt aaaaacaact acaaatccat agaaaatatg gaggtagtct
    23281 taaataaaaa attgaaaatt ctcaaaaata aaaagttaat atgaagctga ctaaagactc
    23341 cggtatgtct aacctcagac aaactgatgt ctaatgttat tgcttagggt atagaactgt
    23401 attagactag gtatattatt ttttcgtaat tatataaata taaagtggca aaggaggtaa
    23461 ttgagatgac aacacattta agttttagac aaggcgtgca agagtgtatc ccaacattat
    23521 tgggttatgc cggtgttggt atttcatttg gtattgtggc ttcgtctcaa aactttagta
    23581 ttttagaaat tgtcttgtta tgtcttgtta tatatgccgg tgctgcgcaa tttattatgt
    23641 gcgcgttgtt tatagcaggt acacctatat cagcgattgt actaactgta tttatcgtaa
    23701 attcaagaat gttcctttta agtatgtcgc ttgcaccaaa cttcaagaca tatgggtttt
    23761 ggaaccgtgt tggattaggt tcattagtaa ctgacgaaac gtttggcgtc gccattacac
    23821 cttatttaaa aggagaagct atcaatgatc gttggatgca tggtcttaac atcacagcat
    23881 atttattttg ggcaatttca tgtgtagctg gggctttatt tggcgaatat atctcaaatc
    23941 cgcaaacgct agggttagat tttgctatca cggctatgtt tatctttttg gccattgcgc
    24001 aatttgaatc aattactaaa tcgcgattaa gaatttacat agtactcatt attgccgtca
    24061 tagtaatgat gttatcgcta agtatgttta tgccttcata tctagcaata ttaattgcag
    24121 ccacaatttc agcagcgtta ggagtgatga tggaacaatg ataactcata tgaacatgtt
    24181 aatacttatt ttattgtgtg gtatcgtaac gctattaatt cgaattatac cttttatcat
    24241 gatttcaaaa gtgcaattgc ctgatgtcgt ggttcgatgg ctatcattta tcccaatcac
    24301 actatttacg gcacttgtca ttgacagcat tattcaacag acgcctcatg gtgaggggta
    24361 tacattaaac atcccttaca ttatcgcgct cattccgacg gttattttat ctataatcac
    24421 gcgtagttta actattacaa ttattagtgg gattgttatc atggcagcat tacgattttt
    24481 cttttaaaaa taactgaaaa tcattgaaca gatatttaaa cttaggtaaa ctgttagtaa
    24541 tcagaaaatt ctgatataca aatcgtctat gttaagattg ggaatcgtgt atacgtaagc
    24601 gatttggcta acgcatgaca atgataacaa cattttaaat gataaaagta attcatcact
    24661 gaatctcaac taacacataa caatttcata tttcttattg tgagaagttg agggacttgg
    24721 ccctgtgata cttcagcaac cgactttata gcacggtgct aaaaccaacg agttactcga
    24781 atgataagta taaagacttc ttacttttca atagggtgag aagttttttt gtttaaggag
    24841 gaaagaacaa tgacaaatta cacagtagat actttaaatt taggggaatt tattacagaa
    24901 tctggggaag tcatagataa cttgcgtttg agatatgagc atgtcggtta tcatggacaa
    24961 ccattagttg tagtttgtca tgcattaact ggcaatcatt taacatatgg aacagatgat
    25021 tatccgggtt ggtggcgaga aattattgat gggggatata tacccattca cgattatcaa
    25081 tttttaacat ttgatgttat tggtagtcct ttcggttcaa gttcaccttt aaacgaccct
    25141 cattttccta aaaaattaac attaagagat atagtcagag cgaatgaacg aggtatacaa
    25201 gcccttggtt atgataagat taatatttta atagggggaa gtcttggagg tatgcaagca
    25261 atggaactac tctacaatca acagtttgaa gtagataaag ccattatcct tgctgcaaca
    25321 agtcgaacat catcttatag tagagctttc aatgaaattg caaggcaagc cattcatctt
    25381 ggtggtaagg aaggtctaag tattgcacgc caattaggtt ttttgacata tcgatcatca
    25441 aaaagttatg atgaacgttt cacgccggat gaagtagtcg cataccaaca acatcaaggt
    25501 aataaattta aagaacattt tgatttgaat tgttatctga cactgctaga tgtattggat
    25561 agtcacaaca ttgaccgagg tcgcacagac gtaacgcatg tttttaaaaa tttagaaaca
    25621 aaagtgttaa cgatggggtt catagatgat ttgctatatc cggatgatca agttcgtgca
    25681 ttaggtgaac gttttaaata tcatcgtcat ttcttcgtgc ctgataatgt tgggcatgat
    25741 ggattcctac taaactttag tacctgggca cctaacttat atcatttctt aaatttaaag
    25801 cattttaagc gtaagtaata atatatattt tcaaagaatg aagccactaa atcacagtta
    25861 tcatattcaa aaacatctag agtacatgag agtggaaatg tattgtaaat ctaaatcaag
    25921 cttgattgct atgtagtggg attaaataat catgttgaaa atgaaagcac acaaaagcga
    25981 ttcaaatgag tatgttaatt acttataaaa catgaaagtt attattcaat taaatgaaat
    26041 agaagcatac aatctataaa ttgttaattt tcattaaaga ggttaaaata atagctatag
    26101 ttaaaaatat gggagtggct tatgtgtttt caaaaataca acctaaagca acaataattg
    26161 caacgattgc gttggtattt gtcgctttag ctttatatct agtgcctggt ttaggactaa
    26221 tatttgcatt atttgcaacc ataccaggta tcgttttatg gaataaatca atacaatctt
    26281 tcgggattag tgcacttatt acagtaatta taacaactgt tttaggtaat actttcgttt
    26341 taagtgccat catattagtc ttaattgcaa gtttaattat tggtcaattg ctcaaagaaa
    26401 gaacgtctaa agaaagaata ttatacgtaa caacagtagc gatgagctta atttcattaa
    26461 tcgcttttat gttactacaa acattcggaa ggattccacc atcagcgagc atagtaaaac
    26521 ctttcaagca aacattacat gaagcgatta cgatgagcgg tgccgatgcg aatatgaccc
    26581 aaatattaga agaagggttt agacaagcga ccgttcaatt accaggtttc atcattatca
    26641 ttacattttt aatcgtctta attaacttaa tcgttacatt tccgatttta cgaaaattta
    26701 aaatcgctac acctgtattt aagccacttt tcgcgtggca aatgagcggt attttattat
    26761 ggatatacat tattgttatc atatgtttat tatttacagg tcaaccgagt gtgttccaga
    26821 gcattctttt aaacttccaa cttgtgttat cattagtaat gtatattcaa ggtttaagtg
    26881 ttattcattt ctttggtaaa gcgaaaggtt tgccgaatgc agtaacgatt ttactattgg
    26941 ttatcggtac aatactgaca cctacgacac atattgtagg actacttggt gttatcgatt
    27001 taagtttgaa tttgaagcga atcatgaaaa ataattctaa aaagtgaata gaggtggaat
    27061 aatgaatcgg cagtccacta agaaagcttt actaatacca tttgtcatca tgatcatcac
    27121 agcaattgtt ttaatgggtg tatggtttat ctttaatagt cttatagcac taattgcatc
    27181 tatcgttctt gtcgtgatga ttattgttag catcatttta ttcagacaag ctttaatgaa
    27241 aatggatagt tatgtagatg gtttgagtgc tcaaatttca acaacaaata ataaagcaat
    27301 caaacattta ccaattggta tcattgtttt agatgaaaat gatcacatcg aatgggttaa
    27361 ccaatttatg acagatcata tggaagcgaa tgtcatttct gaatctgtaa atgaagtatt
    27421 tccaaacatt ttaaagcaat tagatagagt gaaatccgtt gaaatagaat ataatcagta
    27481 tcatttccaa gtacgttatt ctgagaatga tcactgcctc tatttctttg atataactga
    27541 acaagtacaa acaaatgaac tatatgaaaa ttctaaacca atcattgcga cattattttt
    27601 agataactac gatgagatta cgcaaaatat gaatgatacg cagcgttcgg aaatcaactc
    27661 aatggtaacg cgtgtcatta gtcgatgggc aactgagtat aatatatttt tcaaaagata
    27721 cagttccgat caattcgtag cctatttaaa tcaaaaaata ttagctgact tagaagaatc
    27781 taaatttgat atcttgagtc aattacgtga aaaaagtgtt ggttatcgtg cccaattaac
    27841 attaagtatc ggtgttggtg aaggtactga aaatttaatc gacttaggtg aattatcaca
    27901 atcaggccta gacttagcat taggacgcgg tggcgaccaa gttgcaatta aaagtattaa
    27961 tggtaatgtg cgtttctatg gcggtaagac tgacccgatg gagaaacgta ctcgtgtaag
    28021 agcacgtgtg atctcacatg cgttaaaaga tatccttgca gagggtgaca aagtcattat
    28081 catgggacat aaacgtcctg acttagatgc aattggtgca gcaatcggtg tgtctagatt
    28141 tgcaatgatg aataatttag aagcatacat cgtattaaat gagactgaca ttgatccaac
    28201 attacgacgc gtgatgaacg aaatagataa aaagccagag ttaagagagc gatttattac
    28261 atcagatgat gcttgggata tgatgacatc taagacaacc gtagtgattg ttgatacgca
    28321 taaaccggaa ctggttttag atgaaaatgt cttaaataaa gcaaaccgta aagttgttat
    28381 cgatcatcat agacgtggtg aaagcttcat ctctaatcca ttgttgatat atatggaacc
    28441 atacgcaagt tcgacagctg aattggtaac agagttactg gaatatcaac caacagaaca
    28501 acgtttaaca cgtcttgaat caacagtgat gtatgcaggt attattgtag atacaagaaa
    28561 ctttacatta cgaacaggat caagaacatt cgatgcagcg agttatttac gtgcacatgg
    28621 tgcagatacg attttaacgc aacatttctt aaaagatgat gtggatactt acattaatcg
    28681 atctgaatta attcgaactg taaaagttga agataatggc atagccattg cgcatggttc
    28741 agacgataaa atttatcatc cagtaacagt tgcacaagca gcagatgaac tgttaagttt
    28801 agaaggtatt gaagcatcat atgttgttgc gagacgtgaa gataatctga ttggtatatc
    28861 tgcgcgttca ctcggttcag taaatgtcca gttaacaatg gaagcacttg gtggcggtgg
    28921 acatttaacc aatgcggcaa cacaacttaa aggtgtgaca gtcgaagagg cgatagcaca
    28981 attacaacaa gcaattacag aacaattaag taggagtgaa gatgcatgaa agtaattttt
    29041 acacaagatg ttaaaggtaa aggtaaaaaa ggtgaagtta aagaagtacc agtaggttat
    29101 gcaaataact tcttattgaa aaagaattat gctgtagaag caacaccagg taaccttaaa
    29161 caattagagt tacagaaaaa acgtgcaaaa caagaacgcc aacaagaaat tgaagatgct
    29221 aaagcattaa aagaaacgtt atcaaacatt gaagttgaag tatcagcaaa aactggtgaa
    29281 ggtggtaaat tgtttgggtc agtaagtaca aaacaaattg ccgaagcact aaaagcacaa
    29341 catgatatta aaattgataa acgtaaaatg gatttaccaa atggaattca ttccctagga
    29401 tatacgaatg tacctgttaa attagataaa gaagttgaag gtacaattcg cgtacacaca
    29461 gttgaacaat aaagttggat tgaaataaga ggtgtaacca ttcatggata gaatgtatga
    29521 gcaaaatcaa atgccgcata acaatgaagc tgaacagtct gtcttaggtt caattattat
    29581 agatccagaa ttgattaata ctactcagga agttttgctt cctgagtcgt tttatagggg
    29641 tgcccatcaa catattttcc gtgcaatgat gcacttaaat gaagataata aagaaattga
    29701 tgttgtaaca ttgatggatc aattatcgac ggaaggtacg ttgaatgaag cgggtggccc
    29761 gcaatatctt gcagagttat ctacaaatgt accaacgacg cgaaatgttc agtattatac
    29821 tgatatcgtt tctaagcatg cattaaaacg tagattgatt caaactgcag atagtattgc
    29881 caatgatgga tataatgatg aacttgaact agatgcgatt ttaagtgatg cagaacgtcg
    29941 aattttagag ctatcatctt ctcgtgaaag cgatggcttt aaagacattc gagacgtctt
    30001 aggacaagtg tatgaaacag ctgaagagct tgatcaaaat agtggtcaaa caccaggtat
    30061 acctacagga tatcgagatt tagaccaaat gacagcaggg ttcaaccgaa atgatttaat
    30121 tatccttgca gcgcgtccat ctgtaggtaa gactgcgttc gcacttaata ttgcacaaaa
    30181 agttgcaacg catgaagata tgtatacagt tggtattttc tcgctagaga tgggtgctga
    30241 tcagttagcc acacgtatga tttgtagttc tggaaatgtt gactcaaacc gcttaagaac
    30301 gggtactatg actgaggaag attggagtcg ttttactata gcggtaggta aattatcacg
    30361 tacgaagatt tttattgatg atacaccggg tattcgaatt aatgatttac gttctaaatg
    30421 tcgtcgatta aagcaagaac atggcttaga catgattgtg attgactact tacagttgat
    30481 tcaaggtagt ggttcacgtg cgtccgataa cagacaacag gaagtttctg aaatctctcg
    30541 tacattaaaa gcattagccc gtgaattaga atgtccagtt atcgcattaa gtcagttatc
    30601 tcgtggtgtt gaacaacgac aagataaacg tccaatgatg agtgatattc gtgaatctgg
    30661 ttcgattgag caagatgccg atatcgttgc attcttatac cgtgatgatt actataaccg
    30721 tggcggcgat gaagatgatg acgatgatgg tggtttcgag ccacaaacga atgatgaaaa
    30781 cggtgaaatt gaaattatca ttgctaagca acgtaacggt ccaacaggca cagttaagtt
    30841 acattttatg aaacaatata ataaatttac cgatatcgat tatgcacatg cagatatgat
    30901 gtaaaaaagt ttttccgtac aataatcatt aagatgataa aattgtacgg tttttatttt
    30961 gttctgaacg ggttgatata tgttaagttt gtgtattgaa agtgataaat tagtactgtc
    31021 aacgcctctg ttaaagggtt tttaggacgt tgaaaacgat ttgttaaaat gatttttctt
    31081 ttaaaaaggc cgaaaatcaa tgttcgattt ttatttgcat tatggtctcg atattggtag
    31141 aatatcaaat ggttaaatga gaaaaacttg gaggtgctca catgtcatca atcgtagtag
    31201 ttgggacaca atggggagac gaaggaaaag gaaaaataac ggatttcttg gcagaacagt
    31261 cagatgttat cgcgcgtttt tcaggtggta ataatgcagg ccataccatt caatttggcg
    31321 gagaaacata taaattacat ttagtaccat ctggtatctt ttacaaagac aaattagcgg
    31381 taatcggtaa cggagtcgtt gttgatccag ttgcactatt gaaagaatta gacggattaa
    31441 atgaacgtgg cattcctaca agtaatttac gtatatctaa tcgtgcgcaa gtgattttac
    31501 catatcactt agcacaagat gaatatgaag aacgtttacg tggtgacaat aagattggta
    31561 caactaaaaa aggtatcggt ccagcatatg tagacaaagt tcaacgtatc ggtattcgta
    31621 tggcagattt acttgaaaaa gaaacattcg aaagattatt aaaatcaaac attgaatata
    31681 aacaagcata tttcaaaggt atgtttaacg aaacatgtcc atcatttgat gatatctttg
    31741 aagaatatta tgcagcaggt caacgtctaa aagaatttgt aacagacaca tcaaaaatct
    31801 tagacgatgc atttgtagca gatgaaaagg tacttttcga aggtgcgcaa ggtgtaatgt
    31861 tagatatcga ccatggtaca tatccattcg ttacatcaag taatccaatt gcaggtaacg
    31921 ttactgttgg tacaggtgta ggtcctacat tcgtttcaaa ggtaattggt gtatgtaaag
    31981 cttatacatc acgtgttggt gatggtccat tccctactga attattcgat gaagatggac
    32041 atcatattag agaggttggt cgtgaatacg gtacaacaac aggacgtcca cgtcgtgtag
    32101 gttggtttga ttcagttgta ttacgtcact ctcgtcgtgt aagtggtatt acagatttat
    32161 ctattaactc aatcgatgtt ttaacaggcc tagacacagt gaaaatctgt acagcttatg
    32221 aattagacgg taaagaaatt actgagtacc cagcaaactt agatcaatta aaacgttgta
    32281 aaccaatctt tgaagagtta ccaggttgga cagaagacgt aacaaatgtg cgtactttag
    32341 aagaattacc tgaaaatgca cgtaaatatt tagagcgtat ttcagaatta tgtaatgtac
    32401 aaatttctat cttctcagtt ggtccagata gagaacaaac aaacctatta aaagaattgt
    32461 ggtagaactt tatataagtc atacacaatg attataaata catgagcctt ctatctttat
    32521 tggtaggagg cttttgttat gcttgcttct gtatcgattc gattatttag ataaaaaata
    32581 ctaacgtaaa ggcgatattt gctagtcata atttagaaga ttagatgata tttaacgaaa
    32641 attaagatga aatacttgaa tgtaagaagt ctgatgtcga aaatagctat taaaatagag
    32701 tagacgtaag tgtaaatgaa agcacctaaa atagaaaaat ttcaaaaata gcgtaattat
    32761 tataatgaat agactgccaa taaaatgcaa tttttcactt ataacattct tcaaaaaata
    32821 atagcaaaat tatgtaaaaa atatcttgtc atggcaagat tggctgtgct ataatctatc
    32881 ttgtgcttaa gaacggctcc ttggtcaagc ggttaagaca ccgccctttc acggcggtaa
    32941 cacgggttcg agtcccgtag gagtcaccat tttttaggtc tcgtagtgta gcggttaaca
    33001 cgcctgcctg tcacgcagga gatcgcgggt tcgattcccg tcgagaccgt acaaatgcct
    33061 atccaagagg ataggcattt ttttgcgttt aatattatat taataaaaga tatatggacg
    33121 aatgataatc atattgattt atctgttcgt ccattttctt taaaatgtat gaacctcaag
    33181 taacttagtg gttggatatg aaagataaac gtagacaata aaatctttat tagacgtaca
    33241 aacatatgct actgtcaaca tatttcttcg ttgtgatatg ccaccagtcc tccataacat
    33301 caattgttaa agtaacgaat aacgaataat gatatttatt ttctgagcaa tgacgtgcaa
    33361 ctagaagttg ccattatcct aattttatta ttggaataga gacctcatca ttgtgttaaa
    33421 tatcattgtc acaatccgcc gtgagaaact aataaaaaat agtaatatat aagtttatat
    33481 tggaaaatag aattaatagc ttat
//
LOCUS       DAKTGS010000009        32698 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.009, whole genome shotgun sequence.
ACCESSION   DAKTGS010000009 DAKTGS010000000
VERSION     DAKTGS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.009"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(193..1059)
                     /locus_tag="O0286_000381"
     CDS             complement(193..1059)
                     /locus_tag="O0286_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCY0168780.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            1277..2341
                     /locus_tag="O0286_000382"
     CDS             1277..2341
                     /locus_tag="O0286_000382"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCY0168781.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2496..2771
                     /locus_tag="O0286_000383"
     CDS             2496..2771
                     /locus_tag="O0286_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168782.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(2957..3679)
                     /locus_tag="O0286_000384"
     CDS             complement(2957..3679)
                     /locus_tag="O0286_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCY0168783.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            4033..4482
                     /locus_tag="O0286_000385"
     CDS             4033..4482
                     /locus_tag="O0286_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCY0168784.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(4563..5150)
                     /locus_tag="O0286_000386"
     CDS             complement(4563..5150)
                     /locus_tag="O0286_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0168785.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(5128..5226)
                     /locus_tag="O0286_000387"
     CDS             complement(5128..5226)
                     /locus_tag="O0286_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168786.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            5283..6965
                     /locus_tag="O0286_000388"
     CDS             5283..6965
                     /locus_tag="O0286_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCY0168787.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(7094..7477)
                     /locus_tag="O0286_000389"
     CDS             complement(7094..7477)
                     /locus_tag="O0286_000389"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCY0168788.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(7479..8330)
                     /locus_tag="O0286_000390"
     CDS             complement(7479..8330)
                     /locus_tag="O0286_000390"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCY0168789.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(8323..9141)
                     /gene="panB"
                     /locus_tag="O0286_000391"
     CDS             complement(8323..9141)
                     /gene="panB"
                     /locus_tag="O0286_000391"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCY0168790.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            9214..10074
                     /locus_tag="O0286_000392"
     CDS             9214..10074
                     /locus_tag="O0286_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0168791.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(10160..10864)
                     /gene="budA"
                     /locus_tag="O0286_000393"
     CDS             complement(10160..10864)
                     /gene="budA"
                     /locus_tag="O0286_000393"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0168792.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(11142..12101)
                     /locus_tag="O0286_000394"
     CDS             complement(11142..12101)
                     /locus_tag="O0286_000394"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0168793.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            12788..14236
                     /locus_tag="O0286_000395"
     CDS             12788..14236
                     /locus_tag="O0286_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0168794.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(14317..15654)
                     /locus_tag="O0286_000396"
     CDS             complement(14317..15654)
                     /locus_tag="O0286_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCY0168795.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            15912..16328
                     /locus_tag="O0286_000397"
     CDS             15912..16328
                     /locus_tag="O0286_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168796.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            16451..17341
                     /locus_tag="O0286_000398"
     CDS             16451..17341
                     /locus_tag="O0286_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCY0168797.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(17534..19030)
                     /gene="mqo"
                     /locus_tag="O0286_000399"
     CDS             complement(17534..19030)
                     /gene="mqo"
                     /locus_tag="O0286_000399"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0168798.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            19326..19655
                     /locus_tag="O0286_000400"
     CDS             19326..19655
                     /locus_tag="O0286_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168799.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(19715..21313)
                     /locus_tag="O0286_000401"
     CDS             complement(19715..21313)
                     /locus_tag="O0286_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0168800.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(21507..21950)
                     /locus_tag="O0286_000402"
     CDS             complement(21507..21950)
                     /locus_tag="O0286_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCY0168801.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(22231..22443)
                     /locus_tag="O0286_000403"
     CDS             complement(22231..22443)
                     /locus_tag="O0286_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0168802.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(22721..24430)
                     /gene="betA"
                     /locus_tag="O0286_000404"
     CDS             complement(22721..24430)
                     /gene="betA"
                     /locus_tag="O0286_000404"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCY0168803.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(24691..26181)
                     /gene="betB"
                     /locus_tag="O0286_000405"
     CDS             complement(24691..26181)
                     /gene="betB"
                     /locus_tag="O0286_000405"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCY0168804.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            26250..26345
                     /locus_tag="O0286_000406"
     CDS             26250..26345
                     /locus_tag="O0286_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168805.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            26434..26997
                     /locus_tag="O0286_000407"
     CDS             26434..26997
                     /locus_tag="O0286_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCY0168806.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(27185..27871)
                     /locus_tag="O0286_000408"
     CDS             complement(27185..27871)
                     /locus_tag="O0286_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0168807.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(28135..29757)
                     /locus_tag="O0286_000409"
     CDS             complement(28135..29757)
                     /locus_tag="O0286_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0168808.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            29889..30050
                     /locus_tag="O0286_000410"
     CDS             29889..30050
                     /locus_tag="O0286_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168809.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            complement(30066..30176)
                     /locus_tag="O0286_000411"
     CDS             complement(30066..30176)
                     /locus_tag="O0286_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168810.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(30276..30812)
                     /gene="nrdG"
                     /locus_tag="O0286_000412"
     CDS             complement(30276..30812)
                     /gene="nrdG"
                     /locus_tag="O0286_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCY0168811.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(30809..32659)
                     /gene="nrdD"
                     /locus_tag="O0286_000413"
     CDS             complement(30809..32659)
                     /gene="nrdD"
                     /locus_tag="O0286_000413"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCY0168812.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
BASE COUNT        10342 a         6164 c         5029 g        11163 t
ORIGIN      
        1 tgaacaaaaa gttataaaat cacttttatt gtaactcaac cttgttttac attacagaaa
       61 ttaacaatag cgattaaaat tttattttcc aacaataaaa acgcacaaat atttttattg
      121 aggcagctgt atcgcttctc attgtaaaaa tatctgtgca tattatctat ttatctcaat
      181 ctaacgtctt aactaacttg ttgtatcttg taaaatctta tccatagaat gcgcaacact
      241 atcacggtac atctcaccaa atttcaataa atggaccatc aataaatata aacgataaaa
      301 ttcaagtcta taggatgcac cttttgcgag tggataatgt ttattatacg catcataaaa
      361 ttcgctcgta aaaccaccaa ataccgttgt aataccgata tcgaattctc tgtcaccata
      421 taatggcgct ggatcaaata acgccggacg accatcttgt aagaacatat aattaccacc
      481 ccataggtca ccatgtaata aagacggttt actttgatgc ttttctaatt ccgccacaat
      541 ttgacgtcgc actttgtcat atactttgat atcgttggca tcccatagcc ctctatttag
      601 caactcatct ttcaaatggt ccaagcgttt gtcgacaaaa atggtacacc aatcgtcttg
      661 ccaatgatta tcaaaagaaa tatcgccacc ttcataaggt aatgagaagc caaatttgcc
      721 ttcttcttgt tgctgactgt gtaattgagc tacgagttgc cctaattggc gttgactccc
      781 tgaagcccct tcttctaaat acgtcatcac taaatacgca tcaccgttaa cctcgccact
      841 tgcaattact ctaggtgccg tgatacctgc acgttcaaat tcatttaaac ctgcaatttc
      901 tgcagcataa aatgattctt tacgtccacg ttggacaagt aagaaaaatg tatccgtatc
      961 tgtttcgact cgatatgctt cgtttacatc accaccactc actggtgaaa tctctttaat
     1021 atcttttaaa ggtaaatgct ctaaccattg ttcattcata caaacacctt ccaatctact
     1081 tactttatac tataatattg cctaattagg atgatttaat cttaaaaacg ttattcaaac
     1141 ttgcgtcaat tttcaaaact ttttagtttt aactaggaaa ttatgtatgg aaatgctagt
     1201 ctattaaaag aatatatttc acatatatta agttgaggat agtaagcctc aaatataatg
     1261 aaataggagc atactcatgt acaaattaat taaacctttc ttattcaaaa tcgaacccga
     1321 aaaagcacac ggactaacta tcgatgcatt aaaaacgtta caaaagtttc cggttttatt
     1381 cccagtcgtc gataaactat ttacttataa gaatccaacg ttatcacaaa cgatacaagg
     1441 taatacgtac gacaatccaa ttggcttagc agctggtttc gacaaatctt gcgaagtacc
     1501 aaaagcattg gaacaccttg gattcggtgc tttagaatta ggtggtatca cacctaaacc
     1561 tcaaccgggt aaccctcaac cacgcatgtt tagattatta gaagatgacg ccttgataaa
     1621 tcgaatgggc ttcaataata ttggtatgaa caaagcacta agtcatttgc gtaaaaatgc
     1681 ttatcaagta cctgttggta tcaatgttgg tgtgaataaa atgacacctt atgaagcgcg
     1741 ttatcaagat tatataaagg ttattgatac gtttaaacac gacgtttcat ttttcacagt
     1801 caacatcagt tctccaaata ctgaaaatct tcaaaacttc catgataaag atgaattttc
     1861 aatgttatgc caagctttaa cagcatttaa aaaacaacat gatgtaacag tgccaattta
     1921 tttaaaacta acgtctgata tggatttcga tggcttaaaa gcactattac cagcgattac
     1981 tgagacattt gacggtatca tcttagcaaa cacaacgcga caacgagatg gtttaacttc
     2041 tgctaataaa gtcgaagaag gcggtttgag tggtcgtcca ttatttgaac gtaatttaaa
     2101 attgattaag tatgcttatc agcaaacaaa tggtgaattt ttaattatag gtacaggcgg
     2161 cgtattcagt actgaagatg caatcaaaat gatgcgtcac ggtgcgtcac ttattcaaat
     2221 ttattcatca cttgttattg aaggcccagg tttaactaag aaaatgaaca aaggcatcgc
     2281 acgttactta aaagatcatc attttgacaa tgtcagtgat attataggac tagatgccta
     2341 atccaacata gtcaaataat actttaaaac ttgctgaatc gtcttattaa aattatgata
     2401 taactcaaga agatatattt atcaccattt ttcaaaataa tggcttaaat atcacatatc
     2461 attaaatata gcgttttata acggaggcgt ttgttatgac taaagaaaca gataataaaa
     2521 aaacagtagc gacttctgac aacaaagaca cacagcaaca aaatcaacct gaagaagatg
     2581 aacgtcaagg tttgaatggt tatcgtaaaa cagatcttga tttagaaatc gagcaagagc
     2641 tacgtgaaat gatgaaaaca ggagaaaatg aaacgaaaaa tgactataaa aagtttaaag
     2701 tgttttcact tatttcaaca cttgtcattg tcattttagc aattataaga tttgttcata
     2761 aaatgatgta attagagtga gacattgttt tatgtctcag gatccagtta ttcattatat
     2821 ctacaatatt tacgattata taaataaccc gagattttag tatgattcat ttcactaaaa
     2881 tctcgggttt ctatttgata atttttaatg ggatatggca tgtatacgtt cttgcctttt
     2941 atctcatttc caatgattaa tctggatatt gttctaaaaa tgctttcgct tctttattaa
     3001 ctgttttaaa atcaatacct tgttgcatcg ctgcaaagac acatccacaa taacactgcc
     3061 taaagatatt atagtcatta cacatttcta tggaacgctc ataaccttta cttttcttaa
     3121 aatcacttgg caaatagttc acatcgtata ttttttggac atccatacca agttcattga
     3181 ttaattgtgc gttcttttta ggtgataatg tgattgcact gccaaaataa tcatagccat
     3241 gttctacagc tgctttcgct acaatatcca aacgcatttc aaaacaagcg gtacaacgta
     3301 agccgccttc tttttcatca gctaattctt tatccttcac cattttcaca aacttatgcg
     3361 gttcataagg tgcttcaata tacttcacat tcgcaccagt cttgcgatta aaatcttcca
     3421 caaattgttc ttgcacttta gcacgtcgta agtactcatt tttcggatga atatttgaat
     3481 tcgcgaaata aattgcaatg tctgcatatt gtgttaaaaa ctctaatgta tatgtactac
     3541 aaggtgcaca acaactatgc aataagattt taggtctgat tgcttctctt tcccactgcc
     3601 cgattaattt cttcaacacc ttgtcataat taattttttg atttttcatt ttactgataa
     3661 taggttcagc attaatcatg atttaacctc ccacatttaa tcattaactt ctattatata
     3721 tgattcatat taaatgtcag tcaaaaaagt tagaaattca ttttaatgca tttatatttc
     3781 gaaaatccct tatgtatcta aaagcatttt ttaaacttga attttaaaac actaaacaac
     3841 acatacgtct ctgtgtcatt ttcatttttt gtatgtcata tatatgttta cttcatttaa
     3901 atcaatttca tcttataatt tatcgtgtat tttacaaaag attgacttca attcatcgta
     3961 aaagttatac ttttgccatt ttttaatgta acatggtgtt agtaataaaa ataatacatt
     4021 gaggtgtttt acatgacagc attattccct tatatcgctt ttgaaaattc aaaagaagcc
     4081 cttgcatatt acgaagaagt atttggtgca actgacgtta aacgtttaga agttggcgaa
     4141 gaacaagcgt cacattttgg tatgactaag gaagaagcgc aagaagcaac tatgcatgct
     4201 gaatttgaag tgcttggcgt aaaagtgtta tgttctgatt cttttggtcg cgctgacaaa
     4261 attaataatg gcatatcatt attaattgat tatgatgtta acaataagga agatgctgat
     4321 aaagttgaag cattctatga gcaaattaaa gatcattctt caattgaaat agaattaccg
     4381 tttgctgacc aattctgggg tggcaaaatg ggcgtcttta ccgataaata cggtgttcgt
     4441 tggatgttac atggtcaaga ctatactgca atccaacaat aatgttattg ataaagatat
     4501 catttaataa gcttatgtta tttagcaagg caagccactc tcgattcatg gcttgcctta
     4561 ttttattgta tatttttaat tatcgttgct ggcccttgat tgacaatcgt attaaaatgc
     4621 cttattaagt caactttgtc tatacggttt ggatcttcta cccaatgtct tataaaagac
     4681 aatcccgcac ctgaaacata actcatgaaa taagaaaatg gtataccatt aatttgatca
     4741 ttttcatttt tataacgttg taaatgacac ataattaaat cataaagttt ttcgtggata
     4801 tttgaatctt tacccatacg aaacatcaat tgataaaatg cgatgtcttt ttctatcatt
     4861 tctattaaaa cggtcataat ttgatgtatg ttatccgtgg ataacttaac tgctccattt
     4921 aacttctcat catgaatgaa gtctcttatt tcctccaact gctggtcctc taatttttca
     4981 agcaaatcat acttatcata ataatgcgta taaaatgtac tacggttaac atcagctaaa
     5041 tctgcaattt gttgcacagt aatctcttct aattggtgtt gatgtaaaag ttcaataaat
     5101 gcatttctca ttgcaacttg tgattttcta atacgtcgat ctatagtcat ttatatcaag
     5161 tcctccccaa tgattataaa cgttatgttc attatcccac aaatctccaa cattgatgat
     5221 tggcacacaa tgtttacctg tttaatatag gtgatacaaa caaacagaaa aaggtgataa
     5281 caatgaacca acatttacta ggaaatccaa aattaactgt aactcatgtc aatgaagtta
     5341 aagccggtat taaccacatc gttgtcgaca gtgttcaata tggaaatcaa gaaatgatta
     5401 tggaaaaaga tgtcactgtg gaaatgcgcg atggcgaaaa attatatatt aatattttca
     5461 gaccaaataa agatggcaaa ttccctgtag ttatgtctgc agatacttac ggtaaagata
     5521 ataagcctaa aatcacaaat atgggtgccc tttggccaac attaggtacc attccgacat
     5581 ctagttttac acctgaagaa tcaccagacc caggattttg ggtgccaaat gattatgttg
     5641 tagttaaagt tgcattacgc ggtagtgaca aatccaaagg cgtcttatct ccatggtcaa
     5701 aaagagaagc ggaagattat tacgaagtga ttgaatgggc agcaaatcag tcatggagta
     5761 atggaaatat cgggacaaat ggtgtttctt atcttgcggt gactcaatgg tgggtcgcat
     5821 cattaaatcc accacattta aaagcaatga ttccttggga aggcttaaat gatatgtata
     5881 gagaagtagc ctttcacgga ggtataccag atactggctt ttatcgtttc tggactcaag
     5941 gtatttttgc gagatggaca gataatccaa atatcgaaga tttgattcaa gcacaacaag
     6001 aacatcctct gttcgatgat ttttggaaac agcgtcaagt gccattatca caaattaaaa
     6061 cacctctact aacatgtgct agttggtcta cacaaggttt gcacaaccgt ggctcttttg
     6121 aaggatttaa acaagctgca tctgaagaaa aatggctata tgtgcatgga cgtaaagagt
     6181 gggaaagtta ctacgctaga gaaaatctcg aacgccaaaa atcattcttt gatttttacc
     6241 ttaaagaaga aaataacgat tggaaagata cgcctcatgt catttatgaa gttagagatc
     6301 aattttataa aggcgaattc aaatcagcgt cagcgttccc tttacctaac gcagaatata
     6361 caccattgta tttgaatgct gaaaatcaca cattgaatca tgcaaagatt agtagcgcgc
     6421 atgtcgcaca atatgactct gaagataaac aacaagatgt aagttttaaa tatacgtttg
     6481 acaaagatac tgagttagtt ggaaacatga acttaaaact atgggtaagc actaaagact
     6541 cagatgatat ggatttattt gcaggtatta aaaagttaga tcgtcgtggt aatgaagtta
     6601 acttccctga ttttaatcat attgaaaatg gtcaagtagc tactggttgg ttacgcgtat
     6661 cacatcgtga attagatcaa gaaaaatcct caatcgcgca accttggcat aaacatgaaa
     6721 cagaattaaa gttgtcacaa gatgagattg tacctgttga aatcgaattg ttaccttcag
     6781 gcacgctatt taaacaaggc gaaacattgg aagttgttgt aaagggtagt gaaattgtaa
     6841 ttggtaatag tactcctggc atgaaaacac gttatgaaca tgaagaaacc gtaaataaag
     6901 gcatgcacat gatttatact ggtggtaaat atgattcaca attaatcatt cctatcgtta
     6961 attgatatgc agcaattacg gtcgcttttg attaaaagtg acatagtgat aggactgtat
     7021 aacaagagaa agccacacgc ttggaatctt aaaccaaggt gtggcccttt ttattattga
     7081 tggctattta attttataac actatcgtat tttctttttc atgaatcatt tcaataatga
     7141 cattatcttc attcattact gctactttag gtgcatggtt tttaatttct tcttcattca
     7201 actgtgcata agtcatgatt atgactacat cgcctacttc aacaagtctt gacgctgcac
     7261 cgtttaaaca aattttacca ctacctcttt caccagctat tacgtatgtt tcaaaacgtg
     7321 caccattatt attattcacg atggctactt tttcatttgg caagatgtct accgcttcca
     7381 atatatctga atcaatcgta atgctaccta cataatttaa atttgactca gtcactcttg
     7441 ctctatgaat tttagcattc atcattgttc ttatcacttt attcagctcc aattattata
     7501 ttatctatta aacgcgcttt tgaaaattta acagctaacg agataaatat gcgtccagtt
     7561 atttcgtgtt gttctactaa ttgaggataa ctataaacag caacttcttc aatgcgtcca
     7621 cttatatgtg attcaagata ttcagtaacc ctgtctataa ttactttact ttgacgttca
     7681 ccgtcttgat acaacgcttg tgctaatagc aaacttttac ttaaatgtac cgcttcttgt
     7741 cgttcttgct ccgttaaata aacatttctt gaacttttcg ccaaaccatc tgcttctcga
     7801 acgatatcaa taccaataat ttcaacggca tgattgaagt cttttaccat ttgctcgaca
     7861 atagccaatt gctgggcatc ttttttacca aaataagcat aatccggcat aacaatatta
     7921 aatagcttat taactactgt taccacccca tcaaaatgcc ctggacgctt cgctccttct
     7981 aacacatcag ctaatgggcc tactttgaca tcaataccta attcacctgg atacatatct
     8041 tctactgcag gatgaaaaac aatgtccgct cctacttctg atactaattc taaatcttta
     8101 tcaatttgtc tcggataagc atcgaaatct tcgtttggac caaattgtaa tggattaaca
     8161 aatacactca caattgtaat atcatttgta ctaactgatt cgcgtaccat cgttaaatgt
     8221 ccatcatgta aggcacccat tgttgggata aaaccaatcg ttgtgcctga gcgtttggct
     8281 gctttaacaa tgtgttgcat ctcttttacc gtagtaatca gcttagtcat tgttattaac
     8341 ctcattcata attttctttt tatacgtata ttcttctgat ggaaatgcac cagatttaac
     8401 ttcttgatcg tattgtttta aaccatccac accaacacta aaatcagcaa attgcttcac
     8461 aaatttcgct ttatgttcaa caccataatt taacatatcg tgataaacca atacttgacc
     8521 atctgtacct tttcctgcac caataccaat gactggaatt gttaagtgct tgctaatttc
     8581 ttctgctaaa tcatttggaa ttgcttcaag tactaacgca actgcaccag cttgttctac
     8641 atttttcgcg tctaaaataa gttgctccgc tgcttctttc gttgcacctt gtaatttata
     8701 ccccataacg ccaacacttt gaggtgttaa tcctaaatgt gcaacaacag gaataccaat
     8761 tgccgttgct ttttcaataa atggtgtaat atgcgctcct tctgctttaa ttgcatttgc
     8821 attcgtctcc tgataaagct ttagagcatg atttaagtct tgtgtcatag agatgcctac
     8881 tgcaccaatc ggcatatcaa caactacaaa tgtatttggt gcgcctcttc ttactgcacg
     8941 accgtgatga atcatatctg ctaacgtcac ttgtacggta ctttcataac ctaatacagt
     9001 cataccaagt gaatccccaa caagaatcat atcaataccc gctgcttcca cttgtttagc
     9061 acttggaaaa tcataagctg ttaccataga aattttagtt tgcttttgtt tcatatctat
     9121 taattgactt actgttttca atgttattca acctcttttt gcagtattat tagaaatagt
     9181 ataatgaatc ttttatagaa aggaaagaag aagatgttat cagttgcgat tatcggccca
     9241 ggcgctgtcg gtacaactat tgcctatgaa ttacaacaat cattgcctca tacaacgctt
     9301 atcgggagac acgccaaaac aataacatat tatactgtac cacatgcacc tgcacaagat
     9361 attgttgtga aaggttatga agatgtcaca aacacatttg atgttattat tattgcagtc
     9421 aaaacacatc aacttgatgc agttattcct catttaactc atcttgcaca tgaggacacg
     9481 ctcatcattt tagcccaaaa tggttatggt caactcgaac atatttcatt taaaaacgta
     9541 tgccaagcag ttgtctatat aagtggtcaa aagaaaggcg atgttgttac gcacttcaga
     9601 gattatcaat tacgtataca agataatgca ttaactcgac aatttagaga tttggtccaa
     9661 gacagtcaaa tcgacattgt attagaagca aatattcaac aagctatttg gtataaattg
     9721 ttagttaact tgggcattaa ttctatcaca gcactcggta gacagaccgt tgcaatcatg
     9781 cataatcctg aaatacgtac actgtgtaga cagctattac tagatggttg tcgtgttgca
     9841 caggcagagg ggttaaactt ttcagaacaa actgtcgata ctattatgac tatatatcaa
     9901 ggttatcccg acgaaatggg aacgagcatg tattacgaca ttgcgcatca gcaacctctt
     9961 gaagtcgaag cgattcaagg ctttatttat cgacgtgcac gtgaacacaa tcttgacacc
    10021 ccatatcttg atactattta tagcttttta cgcgcgtatc aacaaaatat gtaagcacat
    10081 atataatata aaaagacctt aacgatgcat ttagaaccac ctgttcctaa tatcgctaag
    10141 gtcttttaat tatattagac tattcagctt ctctaatttc ttctgccaca tctttatagt
    10201 ctattttcgc tttaacaaac gtctcgttat ttaccgggaa atgttgttgg aatgtttcaa
    10261 agttttgtat ctcaacgaca acgtcatcca cttcaaagtc aagaacatgt ccaccataag
    10321 ctctttcatc atccgcaaaa tgtatatgaa aaccagcaga ccctacgcca tgaaataatt
    10381 ctggtgtaaa aaatccaaca atggcaccac gaatatcttg tcttttttcc tcaggttgtc
    10441 tgcgtgctga atcaatcaaa cgtgtatatg gcggttgctg agcaggcatc attcgtacat
    10501 gcatatgttt aaatgtgcca taaattttaa cagccgaaaa taaattctca cttaacattt
    10561 catttttaat ttgtgcaaat acatcatctt gtgataattg ttgcaatgga aatgtcttac
    10621 tcgctttaaa attagtaatc gatgcatacg gtactttctc atcgcctttt aattctataa
    10681 attctttatg ctcgttagca tgatatgcct ttccgtctaa aaatattact tcgccatcag
    10741 accctgttaa cgttgcaatc cctaaattcc catgttctaa caattcatta attgtagctg
    10801 tgccttctag taagccagcc attaacgtac ctaatgtacc atgttgatac aagacattcg
    10861 tcattccaaa accatctccc tatagttatt cctcgttgtc atgctctact tcgtgattaa
    10921 tgcagaacta ctttccttta tactcaatga cgcctcaaca tttttattca tgtcactaaa
    10981 ttttcacgag tcataatact ttattcaatt attattttac gcaaaagtat tgcctctatc
    11041 aatgcttgct aaaaaaatgg acctatcagt gccacgtgtt actgcaacac taatagatcc
    11101 tcttaccatt aaaatatttc taattaatac ttaaaatagg cttagtcttc taataaatat
    11161 ttaattgaat caaatgtatc ttctaatgtt ttaacagatt tatcgaacaa cgcttgttct
    11221 tcggcactta atggcatttc ataaatttta actgcgccat gttggttaac taatgttggt
    11281 acacctaggt aaacgccttt gtgaccacca tattgaccat ctaattgtat agaaacattt
    11341 aagacattat tttcattatt taaaatggct tttgaaatgc gcatcaatgc taatgcaata
    11401 ccatagtatg ttgacccttt agcttggata atttcataag cagcgtcacg tgtattcaca
    11461 taaatttctt ccgctttagc ttcgctacca gtttgttctt ttaatgtgtc atatactgaa
    11521 atacctgcta catttgcttg tgaccaaact gcaagttcag tatcaccatg ctcgccaata
    11581 atactagcgt caacacttga aggtgcaaca ccaagttctt ggctaattaa atattgtaaa
    11641 cgtgcactgt ccaatacagt acctgaaccg ataacacgct ctgctggtaa tccagtatat
    11701 tcttttacaa atcttgttaa aatgtctaca gggtttgccg cgattaagaa atatccatca
    11761 aagccactat ccataacact cttaacgatg ctcttcataa tcttagtatt tttttcaact
    11821 aattgtaaac gtgtttcacc tggcttttga ggtgcaccag ctgtaataac aactaaatct
    11881 gcatctttac agtcttcgta ttcacctgct ttcacatcaa ctggtgaagg actgtggact
    11941 gtaccatggt ttaaatcttg aacatctgct tttactttgt cttttgcaat gtcaataatt
    12001 acaaattcat cagcaacacc ttgcgtaacc attgcaaagg catagcttga tcctacagat
    12061 ccatctccga ttaatacaac ctttttacca aatgttttca taattatgtc tcctaaccta
    12121 attgaattta ttttacacgt gactacacgt aaaaatatct atactgaaat taaagattaa
    12181 caattttatt ttattataat attatgctac caaattacaa acataaaact gagatgtaag
    12241 cggagtcaaa ttacactcta tcgccaataa atattgatat ctcaagcttt agcacgtaat
    12301 ttgaaatttg tgacaatttt tagcattcta aaattgcgta acgacgctat ttaaaggctt
    12361 taacaaacta ttgagctttc tatttttttc attttaaaaa ggatggctat tcgacttaaa
    12421 tgacttacta tataacctcc tattttccgc cttaccctca tcatatttga tgcatcattt
    12481 tatagtcaaa gaattacatt tcatacaaac tagccaatca gacataaaca tgaaattgat
    12541 tttaaaaata taatgtgcaa aattgttaga aaattagcaa aaaatagtac ttattcattt
    12601 aatatctgtt ataatcagtt ttatcattta aaaaatctat ggatgttatc aagacaagtc
    12661 attaatacgt aacataaagt tgtgattttt cagaattttt agacaaaata ttgattattt
    12721 gtgctatgat ataacatatt aaaaaacacc gtatagaatt gtttatgaat gtttgggagg
    12781 aaagtttatg ggaagttttt tcaataaaat agcacgaaaa gaggatccgg ctatctatca
    12841 aaataaagat ggtcatttaa agcgtacact tcgggtgcgt gatttcttag ctttaggtgt
    12901 aggaacaatt gtatcgacat ctatctttac gctacctggc attgttgctg cagaacatgc
    12961 aggaccggcc gttgcgttat cattcttact cgctgctatt gttgctggtt tagttgcatt
    13021 tacttatgca gaaatggctg ccgctatgcc atttgcaggt tcagcctatt cttgggtcaa
    13081 tgtattattt ggtgaatttt ttggatgggt tgccggttgg gctctattag ctgaatattt
    13141 tatcgccgta gcctttgttg catcaggatt ctcagcgaat ttacgcggac ttgtgaaacc
    13201 aattggcatc gaattacctg cagcattatc aaatccattt ggtacaaatg gcggttttat
    13261 cgatattatt gctgctatcg ttattttatt aactgcatta ttactatcac gtggtatgtc
    13321 ggaagcagct cgtatggaaa atattttagt tattttaaaa gtattagcta ttattttatt
    13381 tgtcatcgta ggtttaacag caataaatgt tagtaactat gtgccattta ttccagaaca
    13441 caaagtaact gctacaggtg actttggtgg atggcaaggc atatatgctg gtgtttcaat
    13501 gattttctta gcgtatatcg gtttcgattc tatcgcagca aactcagcag aagcacttga
    13561 tcctcaaaag acaatgccta gaggtattct tggttcttta agcgttgcta tcgtattatt
    13621 tattgctgta gcacttgtgt tagttggtat gttccattac tcacaatacg caaacaatgc
    13681 tgaacctgtt ggttgggctt tacgtcaaag tggtcatggt gttgtagcag ctattgttca
    13741 agctatctct gttatcggta tgtttacagc attaattggt atgatgttag caggctcacg
    13801 tttactttat tcatttggac gtgacggctt attaccttca tggttaagcc acttaaacga
    13861 caaacattta cctaatcgcg cactagttat acttactatt attggtgttt taattggttc
    13921 tatgttccca ttcgcatttt tagcacaatt aatttcagca ggtacacttg ttgcatttat
    13981 gttcgtttca ttagcaatgt atcgtttgag aaagcgtgaa ggtaaagatc taccaattcc
    14041 tgcatttaaa ttacctttat atcctgtgtt accagcaatt acatttgttt tagtattgct
    14101 agtattctgg ggattaggat tcgaagcgaa attatacact ttaatttggt ttattgttgg
    14161 tatcatccta tatttaagtt atggacttag acattctaag aaaaatgacg tagctgaata
    14221 ccacccacca aaataaaaaa taaaaaaaat catttcccat ctgattaaca tccagatgtg
    14281 gaaatgattt tttattttat attaagataa tcgctattac caaccttgtc cagatatgtc
    14341 atattgatct aagttacctg cttccaaagc agtcagtgca tcttctatag tgtttaacgc
    14401 cgtgtctaat tgctcatatg ttattaccaa tggcggttgg aatcgcaaca catttcctgc
    14461 tacagctata ataactacgc catgctcaaa gcagtaatta caaattttaa gtgccgcact
    14521 ggcatcacgt gttttgagtt ttttgtcgga aacaatatca ataccaatgc tcagaccttt
    14581 acctctaaca tcgcctacac tattgtattt agatacccat tgatccattc gtttcctaac
    14641 atattcccct ttttccgcac tagcctgaag aagcgactga tcttcaatca tttgaattgt
    14701 ggctaatgca gcttcacaac taactggatt agcacctgtt gtaaataaat gtgctggtgc
    14761 ttctaaacaa ttcataatct ctttgcgtcc aacaattgct gacataggca taccacctgc
    14821 taaggatttt ccgaaagtga ttaaatcagg cgtaaaatta aaatgcgaga ctgaactcca
    14881 tgtacctgtt ctcccaaaac cttgttgaat atcatcgaca gcgattaaaa taccatgttc
    14941 acgacaaatc ttttctaacg cttcaaaata ccctggaact ggttctaaaa gtccaccatc
    15001 gccttgtatc gtttcaataa caatacatgc tacttcgtca gcaggtacat acttcgcaaa
    15061 catttctttt aagggtgcta aatattcttc tactgaatta gcttgtggct gctcgtacat
    15121 accacgatat ttatctggaa acggaatatg ataaaaacca ttcaataacg gaccataatg
    15181 tttgcgcata tttaaactaa tagctgacat agacaatgag ccaaaagttg aaccatgata
    15241 tgcattagtg aaactaatga tataaggacg ccctgtatat gctctggcaa atttaatgat
    15301 gccatcattt gcgtctgatc cggttaatcc gaaggtcact cttttttcaa aatctccagg
    15361 tgcaatctca caaagcttct tagctaaacg taccaacggt tcatgataca tatatgctgg
    15421 tgtataatga ataaatttat caacttgtgc ctttatcgct tctgttactt ctctaggtgc
    15481 atgacctacg ttttgagaac tcgcactcga taacaaatcg atatatgtct tcccctcaat
    15541 atcaaccaat gttgctccat atccatgatc aatcactaac ggataatatt taatacgtcc
    15601 tgatttcgca aaataatgtt catcctcttg aattaattga tgtgctttac tcatatacat
    15661 tcccctccca ttgacagtat taatgtgcaa gcaaagtggc ctctcttaaa tcgattataa
    15721 aatccgattt aaaatcctgc tcaaataccg tcatgcctct atgaatacta tccaatatac
    15781 taataaaatt cagatttgta aatatattct gataattaag aattttatat caatctactt
    15841 tgtttatgcg cataattttg ttcgattagg gaattgttta ctatattcca tttgactctg
    15901 aggtgatacg aatgcgttta aaagtattgt tccatttcat tgctgctatt ttcatatcat
    15961 tcatgttgct atggatgacc atgttgtttg acttaataag caatcaatca catttaaaag
    16021 ctttattatt gaatttagat tttctaattc cttctgacaa tacaccatat attctagaaa
    16081 taatatgtca cttactaatt ggtagcgtca tttattttgt atttgtgttg ctgtttcata
    16141 cttctaaaag gctttactat ctatgttata tccctttatt tttcttattt atagcattgt
    16201 atcccttttt agtatttatt gcgcaacgtc caatttttca atttagtgtg acagaattaa
    16261 tcggttggat tattacacat attttcttta tgtcattaat ggcgttagtc atacctagaa
    16321 taaaataata tttttaatta aactgacaat tatttttcac attttataca cttttcaagg
    16381 tttatgactt gagaaatgtt ctcattttta tcatactgga ggtgtattga aaagttaagg
    16441 agtggaaagt atgaataaag agcaattaga aaaaatgaaa aatggaaaag gctttattgc
    16501 cgcattagac caaagtggtg gtagtacacc aaaagcactt aaagaatatg gtgtaaacga
    16561 agatcaatat agcaatgaag acgaaatgtt ccaacttgtt cacgatatgc gtacacgtgt
    16621 ggtaacttca ccttcattct caccagataa aattttgggt gctattcttt tcgaacaaac
    16681 aatggatcgc gaagtagaaa gcaaatacac tgcagattac ttagctgata aaggtgttgt
    16741 tccgttctta aaagtagaca aaggtcttgc tgaagagcaa aatggtgttc aattaatgaa
    16801 accaatcgac aacttagaca atttattaga ccgtgcaaac gaacgtcaca tttttggtac
    16861 aaaaatgcgt tctaacattt tagaattaaa tgagcaaggt atcaaagacg ttgttgaaca
    16921 acaatttgaa gttgctaaac aaattattgc taaaggttta gttccaatta tcgaaccaga
    16981 agttaatatt aatgcaaaag acaaagctga aattgaaaaa gtattaaaag ctgaacttaa
    17041 aaaaggttta gatagcttaa atgctgatca attagtaatg ttgaaattaa caattcctac
    17101 tgaaccaaac ttatacaaag agttagctga acatcctaat gttgttcgtg tcgttgtatt
    17161 atcaggtggt tacagcagag aaaaagcaaa tgaattactt aaagataacg ctgaacttat
    17221 cgcaagtttc tcacgtgcat tagctagtga tttaagagct gatcaatcta aagaagaatt
    17281 cgataaagct ttaggtgatg ctgtagaatc aatctacgac gcatctgtaa acaaaaacta
    17341 atttatacat taaactattt tttgatataa actactaaaa aggactcata catgtgcttt
    17401 aaaggcattg tgtatgagtc ttttttgatt aaagttgatt ttgccgtatg tattagatta
    17461 cgtaaaaaaa tcggacagct gaaaaatctt taagatttct cagttgtccg attttaaata
    17521 tagctattag aatttagttt tcgtagtaac ctaattctaa gtcttttgaa gtttgtttac
    17581 ggatttttct cattaatttt tcgtcttcaa ttaatgattc accgtatgat ggaatcattt
    17641 tcttaatttt aggtgcccat tcagttttgt attcagggaa gttacgttct aatacttcta
    17701 acgcaactga aactgaagtt gaagcccctg gtgattcacc taataatgca attacagtgt
    17761 ggtcttgtga gttaaccact tctgtaccga attggatgaa tcctttaccg tgttcaggtg
    17821 tatctttgat aacttgtaca cgtttaccag cagtgtataa ttgccaatct tcattacgtg
    17881 cttctggata gaaagtacgt aagtggttca tacaaccttc ttttgtcata attacttggt
    17941 caaatgagta tttaattaaa ggtaagtttt taactgctgc tgctaataaa gttgtaatgt
    18001 tgtatgtttt aacagacttg aataaatcta agttagaacc atttttcaag aatttaggtc
    18061 caacattagc aaatggtcca aataataatg ttctttgacc atcaatgtaa cgcgtatcta
    18121 aatgaggtac agtcattggt ggtgtaccag gtggctcttt accataaact ttggcatcgt
    18181 gttgttcaat aacttgtggg tttgtacaag ctaagaattg accactgata gggaatccac
    18241 ccaaatgttt actttcaggg atacctgttt tttgtaataa tggaattgct ccaccgccag
    18301 caccgatgaa tacgtagtca gttacttgtt tgaatttctc accagttagg cgatttttaa
    18361 cagtaacttc ccattgacca tttgataatt gttcaaaatc aacaacttca tggttaaatt
    18421 gcactgtagc atttggatgt gcttcaatgc ttttagccat tttacgtgtt aattcaccga
    18481 agtttacatc tgtaccttcg tcaattttac ttgccgccat gataccaggg ttatcttcac
    18541 ggcctttcat catcaatgga atccattttt tcattacttc gatgtcttca gtatattcga
    18601 tattatcgaa catagggaaa gctttcatcg cttcgtaacg atcttttaag aatttaacat
    18661 tgtttttacc tctaacataa ctgatgtgtg gtaatggatt gataaattct cttgggttct
    18721 cgatgctacc gcttttcact aagtgacccc agaattgttt tgaaatctca aactcttcgt
    18781 taatcacttt cgctttttcg atgtcgatag aaccatcagg ttgtaaaact gtgtagttca
    18841 actcacataa tgctgcatga cccgtaccag cattatttct ttcgtttgaa ctttcgattg
    18901 caggacgatc caagcgttcg taaacgtgga tattccagtc tggctcaatt tcttttaaca
    18961 ttgaaccaaa tgttgtgcta agtacaccgg ctccaattaa aacgatgtct ttactattag
    19021 acttagccat tggtttcacc tctccaaaaa ttgtaaatgt gtatcatcaa tatgaaagtt
    19081 acataaaact gacatatttc tttaaaatat caacgccatt gataacttcc tgttttaatt
    19141 gatacgctgt aacaaaatac tatagttagt gcttacatgt atatgttaaa gcaagcagtg
    19201 gtaaatgtaa attataatta ttcattaact ttgcaatata ttaaatcttt tattcataga
    19261 agataaatat caaatcaatc ataattattt gacaacaaat agctaacgat tgttttaatc
    19321 tacatttggc ttatagcatt ttaaacctat actctatttt gatacaatat aagtgtaaaa
    19381 tcaatcataa aaaggatatt caatatctgc atccaagaaa aacattacaa ttacctttaa
    19441 tattgaaaat tattttgaat cttgcactta taggtattgt aattatcgtt ctaacacata
    19501 ctgagtttca acttaatgcc ggtgatagta atgtgttttt attaaactgg gatattcgag
    19561 gtttaacatc ttatattttc tcattcttaa tactggttgc gcttgtttct aaactaattt
    19621 taatatttat aactagacga caaagaacat cataaataaa caagcagtcc agtgcgtatc
    19681 atcatacctt acgcattgga ctgctttgct atgtttattg attgctatct ttttgttgtt
    19741 gccatttttc tatttcagca tctcgtagtt caacacgacg aattttacct gagtttgttt
    19801 ttggtaagtc gtcaacgaat tcaatctctc tcggatattt atatggtgca acttcatttt
    19861 taacaaattg ttgtagttct ttaactaacg tatcatcacc cgcagtatgg tcctgtaaaa
    19921 taacgaatgc tttaacaata tttcctcgta tatcatgagg ttttgctact acagcacatt
    19981 ctttaacggc tggatgattc gttaacgcat cttcaacttc aaaaggccca atcgtatagc
    20041 ctgaactaat aataatgtca tctcgacgtc cttcaaacca gaaataacca tcatcatcta
    20101 catgagctaa gtcaccagtg atgtagtatt tacctgtttg cgctttcgcc gtacgttctg
    20161 gctctttata ataccctttg aaaagtgctg gcaaatcaag tggtactgca atattccctt
    20221 tcgtattagc aggtacgcta ttcccctcat catctactac agtgaccgaa ctacccggaa
    20281 tgcctttacc cattgatcca atcctctgtg gtgtatcttt taaaaagcct ataagcaagg
    20341 tactttcagt ctggccatat ccatctctta cagttaaatt aaagtatttc ttgaattgtt
    20401 caactacttc tcgatttagt ggctcacctg cagaaacggc actatgtaaa tgcgttaagt
    20461 cataatcatt taagttctgt aatttagcca tcatacgata ttctgtcggt gtacaacata
    20521 aaacattaat ttgatatttt tgaagcaatt ctaagtatgt ttcaggactg aaccttccat
    20581 taaatacaaa agcagttgca cctgaaccta atacagataa gaaaggactc catacccatt
    20641 tttgccaacc tggtgctgct gttgcccaaa ctaagtcatc ttcattaata cataaccaat
    20701 gttttggtgc catttgtaaa tgtgcaaatc cccatccatg acaatgtgta acggctttag
    20761 gattgccagt tgtaccagat gtatatgaca gaatcgccat atcatcacgc gtcgtatctg
    20821 ccatttctag tttgttactt gcgttttctt tttcagcttc aagtgaaatc catccatctt
    20881 tttgaccggc aataacaaat ttagttaacg catcatattc tttaattttt tcaaattcaa
    20941 ctgtgaatgg ctctagtgca ataactgcat taatttcacc atgtgtgata cggtattgta
    21001 aatctttagt tcttagcatt tcagaacatg gaatgattgc aacacctaat tttaaagcag
    21061 caatatataa ttcatacgtc gcaatagatc gtggcatcat aatgagtact ttatcgcctt
    21121 tagataaacc gtgcgatgct aaaacattac ctactttatt agactgttca atgagctgtt
    21181 ggtaagtgac tgatatatct tcgccttcag tattatgata taaaattgcc tttttatctg
    21241 gtatgtggct atatttttcg atttccgaaa taatgttata tttttcaggc gcgaatagag
    21301 ctgacttttg cataactaac ttcctttcat acatccactt ttcctgtgat gaacattgta
    21361 attttataaa tgaattatat acatcatacg cctatcttta cagaattttc aattaaatag
    21421 ggttaaatac caaagtcctc gacactacac tttgacatga cgtagcattc aaggactttc
    21481 aaatgattga gggttgatat ctcgggctag accatatcag ctaattcaat acgaatattg
    21541 tatgataatt cacgattaat tatttttaca tctgcacctt tcgaagtgcc acgatgcttg
    21601 tgtgtatgct tgtactcagc tgaattttgc caatgataaa atgcttgcct attttcccac
    21661 agcgtaataa tgatatagtg tctaccagct gttctaggtc ttaaaaacct taatgcttta
    21721 aatccatcaa cgttttttaa atgcttatta cgtcgcaaaa atttttgctc gaatgtttct
    21781 tcttgtcctt cgttaatata taaatgattt aacacacata atgaatcatt tgataaatca
    21841 tttatcgctt ctagcacatc gtaatatgca gtgtcattat ttttttgtat tgtgagacaa
    21901 tcatccagtt cttctattac ataacttcta tattcatcat aaattttcat aataaatgcc
    21961 ttcatttcat ttatattttt ggtcatatta ctttatatct attactaatt cattcccgta
    22021 tttattaatt acaatcatag tttggcttct ttttaaaaga taagactttg taaaaagtat
    22081 taatatttca tgcaaatggg ggacaggagt cgcccactat ttttgtgtct tcaatttcat
    22141 gatcattatt taacattagt catgaaaata gccgacaact tcatctgtga aatcaccggc
    22201 cttttatttt agctaacttt atttctgatt ttacgatttt aattgatcat acagagaaag
    22261 tgatcttttt acaatttcta aaaactcatg atctatattg gacatttgat gaaaataaga
    22321 caaaatgttt tctgttagct tctcttgttt tgggaatgaa tcatcttctt taatccaaat
    22381 cgctaattcg cctaatggtg ttttatcatc tttaaatgtt tgtatatatt cgtaaaagct
    22441 catagtattc cttctctcaa tttacttata taaatcctac cacgaaagct ttcaagaaaa
    22501 cacaattaaa tgtctattta gtgaactttt taaggttgtg cactctttta atgtctgcca
    22561 attaggtcaa ttaatcatca caatgtacaa ttaactctat tttcagttca tatactcaca
    22621 caccgttttt gaacaacaca ttaacttctc atttagataa aacgcaaaaa agcctggcac
    22681 caatacaata gatgccagac taagagtcta ctatataaat ttatttagcg tatggtttta
    22741 cttcgattgc accttcattt tcatcatgaa caccatgctt ataataatca atatattgtg
    22801 gctctaaagg ctttctgcca cgtataatgt ctgctgcttt ttcagctaac attaaaacag
    22861 gtgcgtgtat attgccattt gtcgtacgtg gcatagctga tgcatcaact acacgtaaat
    22921 tttccatacc gtggactttc attgttaacg ggtcaactac tgccattgga tctgaagcag
    22981 gacccatttt agcactacaa gatgggtgta atgctgtttc accatctcta cgaacccaat
    23041 caagaatttc ttcgtctgtt tgcacttctg gtcctggtga aatttctcca ccattgaatg
    23101 gatccattgc tttttgagat aagatatttc ttgctacacg aattgcttct acccattctt
    23161 ttttatcttc ttctgttgat aaataattaa agcggatact tggtttttcg aatggatctt
    23221 tagatttgat tttcaagcta ccacgagagt ttgaatacat tggtcctacg tgaacttgat
    23281 aaccatgtgc gaccgctgcc ttttgaccat catatcttac agctattggt aagaaatgga
    23341 acattaagtt aggataatca acttcgttat ttgaacgtac aaatccgcca ccttcaaaat
    23401 ggttagatgc tgctgcacct gtacgtgtga aaatccattg taaaccaata aatggcatgc
    23461 gcttgatatc taagcttggc tgtaatgata caggttcctt acatttatgt tgaatgtata
    23521 cctctaagtg atcttcaaag ttttcaccca caccaggtaa atgaacacgt ggctcaatgc
    23581 cttttgattt taggaactct gaatcaccga taccagataa ttgtagtaat tgtggcgtat
    23641 tgaatgcccc accagacaaa atgacttcat tagcatcgat ggtatgtagt ttgccatttt
    23701 tcttatacgt aacaccagtt gctcttctac cttcataatg aatttcagtt acaaaggcac
    23761 gtgtttcaac ggttaagttt ttacgcttca tcgctggatg taaatatgct cttgaagctg
    23821 acattcggcg accacgatgt acttgactat cgaacggtcc aaaaccttct tgtctaaatc
    23881 cattcacatc aggtgtttta tgatagcctg cttcaacacc tgcatcaaag aatgactgga
    23941 ataaaggatt cgttgctggc cctcgtttta acttaattgg tccatcatgg cctctaaatt
    24001 tatcataagg cgctgcaccg tatgtttttt ctaatttttt aaaatacggt aaacagtgcg
    24061 caaaatccca agtttccata ccttctggtt ctgcccagcc ttcatagtcc attggattac
    24121 cacgttgata aatcatgcca ttaatcgaac ttgatccacc taaaacttta cctcgggcat
    24181 gtgctacttt acgaccgccc atatgtggtt cttcatctgt tgaataaatc caatcgtaaa
    24241 atttattgcc tgaagggaac attaacgcag caggcatttg gataaataaa tcccaaaaat
    24301 aatcactgcg acccgcttct aatactaaga cttctttatc tttatcttca ctcagacgat
    24361 tacctagtac agaacctgca ctgcctcctc caatgatgac ataatcatat gatttgtttt
    24421 tgttactcat cgttatgacc tccaaagttt gttgtatttc aaagtgagta ttgcatgaaa
    24481 tatttgttag tggctgattg gttatcgttg aattgctaaa cttagctaac caaaacactc
    24541 gcttttcctt ttacgattgt tactaacagt aatgaaataa tctatgattc attttgcgtt
    24601 tagtccaaat tatcaatcaa catttcgcca cttatacaaa acaaagtgag tgtcttgtgt
    24661 tcttacaatg gcactcacct tatctaattt ttatttgcta aaccaattca ctaattgtgg
    24721 atttgtattt gttaaaatgt gttttgaaac aaggtactct tctaagcctt ctttgcctaa
    24781 ttctctaccg atacctgatt gtttgtatcc accccatggc gcttgtgcaa aatatggatg
    24841 gaaatcatta atccacaccg ttccaagttt caacttgtta gcaacgcgtt gtgcttttcc
    24901 aatatcttta gaaaatacag cacctgctaa accatatata gaatcattcg ctaattgaat
    24961 cgcttcttgt tcagtttcaa agccttctac agtaacgaca ggtccgaaaa cctcttcttg
    25021 tacaatacgc attgacgtat cacaatttgt aatgactgtt ggctcgaaga atagaccatc
    25081 ttttaaatca tctctatctg gacgtttacc accaacagca attgttgcgc cttctgcttt
    25141 agctacatcc atataagatt cgatcttatt acgatgttct gttgaaatca ctggtcccat
    25201 ttcagtatca gcatcaaaac cattacctaa tttgattttt ttcacgcgat caataagtgc
    25261 ttgctcaaat ttgtctttaa tactgttttg tactaatatt cttgatcctg ctgaacaaac
    25321 ttgacctgca tggaaatatc caccatttaa cgcttggtct actgccaatt caaaatcagc
    25381 atcatcaaag ataatgtttg gatttttacc gccaagttcc aaggcaatat tcgtaacatt
    25441 attagcagca tttttcataa tatgcttacc agtctcaatg ccacctgtaa atgatacaag
    25501 gtcaacctct ttatgacctg acattacgtc accaacttca gaacctgcac ctagaataag
    25561 attaattgtt cctttaggga aaccaacttc ttccattaat tcaaaaacac gtattgttgt
    25621 taatggtgta atttcacttg gtttcataac tagtgaacaa cccgtagcaa gcgctggcgc
    25681 aattttccat gatgcttgta ataacggata attccaaggt gtaatttgtg taactacacc
    25741 tactggttct ttaacaattt tgctttctgt atctggaatt ggtgaatcaa tcatttcgcc
    25801 accgtcttta tctgctaatc cagcaaaata cataaacaca ttatgaatat catccatatc
    25861 tgcatatgat tcttctaacg tttttccagt atctaatgtt tctaatcgtg ctaacgcttc
    25921 gcgatgttct ttaatcttgt ccgcgatggc acgtactttt ttacctcttg tttcagcagt
    25981 ttcttgcgac cattcaccag actcaaacgc acgtcttgca gctaagattg cacgttctgc
    26041 atcctctttt gtcccttcag aaaccgtaaa tatcacttct tgattgtaag gattgataat
    26101 atctcttgta tttttattcg cgctttcaac ccactcacca tcaatatatt gacgctgaga
    26161 taaatgtttt aaaagttcca ttcaacaacc tccgttaagt ttgttaaatt tttatttaac
    26221 gaattttaca aacttatgtt agcatagaaa tgaaaaatgt gtcaatttac aaaacaactc
    26281 aagaattaag ttttgtaagt tcgttagatt tttatttaac gaaacattta actttaatgc
    26341 tttaaatatt tttaatgtct ttttttagaa aaggttttat aatgtattag tatttatttt
    26401 aacgtgacca aaatgatgta cggaggtgca atgatgactc gttctcagaa caaacagcaa
    26461 caattagaac aagcaaaaga tattgtaatt aattcaattg gacagacaat ggatttgtat
    26521 ggcactaata gaagtgtagg aaacttatat ggcacaatgg tgtttgaagg cagtatgacg
    26581 cttgatgaaa tgcgtcatca attgcaaatg agcaaaccaa gtatgagtgc tggcgttaag
    26641 aaattgcagg aatttgatat tgttaaacaa caatttacga gaggaagccg caaacaacat
    26701 tttatcgccg agaaagactt tttcatcttt ttccgaaact ttttcactaa aaaattccag
    26761 cgtgaaattg acatcaatgt cgaagcagta aaagatgctc aagctattat caattcatta
    26821 cttgaaagca gtgatttaac tgaagctgaa acacaagagg cacaaaaaat taaatctcag
    26881 cttgatcata cacatgttta ttacgaatgg ttagaacagt taaccgaagc gattgaaagc
    26941 ggtgaaattt tcaaatattt cccaattcca gaacagcctt ctgattctga aaattaaaat
    27001 tgactcaaac aacgacaatg atgattatgc atgataaaaa caatgcaata ggtccacgaa
    27061 ataaacggta atttctatct caataaatag aaattaccgt ttttgtttta tatgacaatc
    27121 aattatggag gtattttcta gggctttctt agattcgtta cattgcttcg ccaatatatt
    27181 tcaattagct attctcgccc tcacgtactc tatcaataat attttgtatt tgttcacgtt
    27241 tttcatattc attttctaca aaataaacta acggttcatc tttgatagta acccaaggta
    27301 caagtacatc aaaatctaat tctttaacca tctctaaact ttgaatagat ttttctctat
    27361 cacttgaacc taatataact gtacgccatc tcttcccttc aaaacatata aaatctccag
    27421 taaataagta acggtgatga ccatcatccc ataaaaatag tgtcgtccct ggtgtatgtc
    27481 caggcgcggg aataacttct aaatccttat ccaacattac acgattacta aattgttttt
    27541 gaacagaaat cttatgtttc agtgcagcca catcattttc atgtataaag taaggggctt
    27601 caacttgatt agaacctcct atagattcat gatcatgatt catcaatact tttgaaacac
    27661 cacctaatga tgcaatgtct tgttgggagt cacctaagtg tccggaatga tagataacaa
    27721 tattacctgt ttcacgtttt aaaataaaag atttaaatag aaatcgatta tcaaaaggca
    27781 gttccgaagt aggtgtcgca tataagtttt ttgtgatgga ttctaaactg tcatgtaatt
    27841 tggactgttt atttttaaat tgattagtca ttttatttca tccttcgatt aatttgaatt
    27901 gtacatacgc agtacccttt atttaattta ataatcaatg agagtctttc aaacttcgat
    27961 ttagaatatg cttcatacaa taaataagta cttggagcac taaaaaatta aatcttttag
    28021 tacaacatta atgaaaacct aaaagttcat cctacaatgc tactaaaaaa aggggaatgg
    28081 aacagaaatg atattttcac aaaattcatt tcgtcgtccc agacccgctt tgaattataa
    28141 attatcgtct tgttcttctt ctgatacttg aacgattcgc aatgaacgac gttcaacttc
    28201 ttttaatttt tcagcacgcg tttcaagttt aattctatcg cgccctaaaa tgattaaaaa
    28261 tgatatcatc atgaaaataa aaataacaat taatggcaca cttgccagta ttgaagcagt
    28321 tttcaatact tctaatgcac gttcaccacc aactagcatc aatgaaaatg gcaataagca
    28381 caatgcaaat gcccagaata aacgattggc acgtaatggt tcgcctacca cttttttctg
    28441 agatgctgcc gctaaaatat atgaacccga atcaaatgtt gttgctaaga ataagaaagc
    28501 agatactaag aatagtacaa tcatcaatga tgggaatggt aaatgatgca ccacttcaat
    28561 aatggttgcc tctgtaccat gtgtatttaa atattgtgtt acattaaact gtccagaaat
    28621 ttgtaaatac acagcatagt taccaaaaat accaaagaat aatacgcatc caagcgttcc
    28681 ataaataatt gttcctagca cgacttcttt aaggcgtcga ccttttgaaa ttctagcgat
    28741 aaataaaccg ataaatggcg catatactaa ccaccatgac cagtagaata ttgtccagtc
    28801 ttgtgggaaa ttcgtttctt ttcgaccttt aataccaccg aatggttcta accatgttgc
    28861 catatgaaag aaatctctca acatatttcc gaaccctgtc actgtcgttt ccataataaa
    28921 aacagtcggt ccaataataa atataaaggc taaaagtaca aaggatagcc aaacgttgat
    28981 atcacttaac ttttgaatac cttttttcaa tcctgtatat gaactaatgg caaatataac
    29041 cgtgattgtt aataaaatgg ccgaacgtaa aatcatattt ttaccatcta aaccagttaa
    29101 tctttctatg cctgcagaaa ttaatggcac acctaacgct agtgatgttg ccgcaccacc
    29161 tagcaatcca aagataaata agatatctac aactttacct acaaatttat ctgtttgacc
    29221 ttttaaaatc ggacgacaag cttgactaat tttatacacc ggttgttttt taacaaatac
    29281 taaataacca attggtaatg ctggtagaac ataaatagcc caagcaattg gcccccagtg
    29341 gaacatacca tattgcgtcg catattggag tgcttcatca ctcatacttt tcgcgccatt
    29401 tggtggaact tgatagtaaa aagcccattc aataacgccc cagtataaaa tatcagagcc
    29461 tatgcctgca caaaacagca ttgccgccca tgtaaatgta ttaaattctg gtttatcact
    29521 tgctttacca agtgtgacat taccatattt accaaatgcg atatacatta caaagcaaaa
    29581 aatcgccagc cccataaata aatatatcga accaattgaa tcagaaatgg cactattaat
    29641 accagtgatg atatcttcac ttgcttttgg aaaagccatc ataggtataa ctgcaaaaag
    29701 aagtacagct actgtcccta taaaggtcgt ccagtccata actttctctt ttttcaattg
    29761 tgctccccct aattattaat tttatgaatc ctgtttcgat ttatctcaaa atgtaataat
    29821 tatattgatt cacaaaattg acaataacta acatttaata ataatgcaaa tttcatacaa
    29881 tttgaaactt ggcaattatt gaatatttat ataatttttc ccgtaataaa caaaacctta
    29941 atagcgctaa aataacagtg ttaagttacg atttaacgaa tttaacaaat tttactagaa
    30001 tggcatttaa gaatatttat acgttattaa cgaatattta tttattgtaa aacgctacca
    30061 aaaagttaga cttccttccc actaaaatac cacttttctc tttcaacttt tttaaaaaac
    30121 ggatatgcaa cttttagtat tggtatcaaa atgattgtta ggtcatattc tatcaatata
    30181 tttttataaa gaattgcttt tattaacttt caattatgta cctaacctaa aaagaagcca
    30241 aggcaacgaa tgttaccttg acttctaata catattcaac taactatata ttcaatcata
    30301 cgcgcatgcg agagtgattg ttgtacatct ataatgcgtt gatttaaaga acctttatat
    30361 ggtaaatcag gtttgaataa gtgttgtata aatagaccat ctactaaaac gtcaatgtat
    30421 gataataact ctcgacgttc tgtacaatca tttgctaaat attcatataa aaatccagtc
    30481 catacccaaa ttgtctttgt atttccaaaa cgtgctcgaa atgctttgac aagatttaat
    30541 gtaatatcca aattacaaaa tggttcgcca cctaatagac ttagcccaga tatataatca
    30601 tgatcgcaat catctaatat ttctgctaat atttcatcag tgtatttctc gccatatctg
    30661 aacttttgtg aggctttgtt ataacatcca acacaattaa atggacatcc tgatacataa
    30721 acactgcatc ttactccttc accgtcaaca aagctatttg attctatttt agcaatataa
    30781 ccttgtcctt gtttaatgtc taaaagtatc attctttagg cgctttcata tgttttactc
    30841 gtgcgcaaat ttctttatga cggcctttaa ttactggacg ttgaactgga ttgcctaggt
    30901 aaccacatgt tcgtttaacg acatcaactg ttttaggatt atcattgcca cagttcgggc
    30961 atttaaatcc tttttcagtt gcttcaaaat ctccatcgta atcacattca taacaatgat
    31021 caatcggaat atttgtacct aagtaaccaa ctttgtcata agagtagtcc cataccgctt
    31081 ctagtgcttt caaattgtgt tgcaatttcg gatactcaca atagtgaatg aaaccaccac
    31141 tcgcataata aggataatct ttttcaaaat ctaacttttc aaaaggtgta acatctttac
    31201 gtacatcata atggaaagag ttttgataat atcctttatc tgtaatgtct ttaatatctc
    31261 caaatctctc ttggtctaaa cgacaaaaac gatccgttag cgattcactc ggcgtactgt
    31321 aaatactgaa ccaaatgtca tataattctg tccatttcgt ttgataacgt ttcatttctt
    31381 taagaatttc aagcgtaaat gcttttgctt cttgagatgt ttcccagtct ggaccataga
    31441 aaacagtagc tgtttcatac aaccctatat agcccattga aatcgttgca cgtttatttt
    31501 taaataactc agcaacatca tctgtttctt ttaatttata gttaaatgcg ccacttttat
    31561 ataaaatcgg tgcgttattc ggtacagcat ctttcaaacg atttatacga taaagtaatg
    31621 catcatgtaa cacatcgata cgttcataaa agatttccca gaatttcgtc atattaccgg
    31681 cagattctaa tgccattcta ggtaaattaa gtgtaacaac accaagatta caacgaccat
    31741 tattttcaaa atgaccttcc gcatctttcc aacttggtaa aaatgaacga caacccattg
    31801 gcgctttgaa atcacctaat atttctacga gtttgtcata atttaaaata tctggataca
    31861 tacgtttcgt tgaacacttt aatgctagtt gtttaatgtc atagttcgga tcttgcggac
    31921 taaagttggt tcctttttta attgaaaata caagtttcgg gaaaatcgct gtcgtgcggt
    31981 cttttcctaa gcctttgata cgagtattta agatagcttg ttgaattttg cgacttaaat
    32041 gatctgtacc taagccgaat cctaatgtta caaaaggtgt ctgtccatta gatgtatata
    32101 aggtattaat ttcatattct aaactttcaa tcgcatcatt gatgtcttta gtgacttgtt
    32161 gatcaacata acgatcaatt tcagattctt tgacaaattg ctttgcgata ttcctatgtt
    32221 gttcttcatt atgtcgtgca tatgtactaa gtaattcgtc aacgcggtca accgtacagc
    32281 caccatattg actgctagaa acattggcta taatttgtac aagctgcgct gatgcagttt
    32341 gtattgattt tggtgaagtt acattcgcgt tgcctatttc aaatccatta tgtagcatat
    32401 ttttagcatc tattaaacaa cagttagtta acggttggaa tggatgataa tctaagtcgt
    32461 gaaaatgtat gtcccctctt tgatgtgcat ctgcaacgtg ctttggtaat aaatgattta
    32521 atgcgtaaga tttagaaacg atacctgctg ttaaatctct cattgttgaa aatgtatcac
    32581 tatctttgtt agcattttcg ttaacaatag caggatcttt agaaatcaaa cccgttaatg
    32641 cttcttcgat ttgattcatt ttaccccttc ttcctatata ttgtgtctac tcattacg
//
LOCUS       DAKTGS010000010        32183 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.010, whole genome shotgun sequence.
ACCESSION   DAKTGS010000010 DAKTGS010000000
VERSION     DAKTGS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32183
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.010"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            216..1082
                     /locus_tag="O0286_000414"
     CDS             216..1082
                     /locus_tag="O0286_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0168813.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            1102..1602
                     /gene="queF"
                     /locus_tag="O0286_000415"
     CDS             1102..1602
                     /gene="queF"
                     /locus_tag="O0286_000415"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCY0168814.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            1943..3448
                     /locus_tag="O0286_000416"
     CDS             1943..3448
                     /locus_tag="O0286_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCY0168815.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(3526..3627)
                     /locus_tag="O0286_000417"
     CDS             complement(3526..3627)
                     /locus_tag="O0286_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168816.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            3718..4635
                     /locus_tag="O0286_000418"
     CDS             3718..4635
                     /locus_tag="O0286_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCY0168817.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(5259..5801)
                     /locus_tag="O0286_000419"
     CDS             complement(5259..5801)
                     /locus_tag="O0286_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCY0168818.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(6152..7210)
                     /gene="hisC"
                     /locus_tag="O0286_000420"
     CDS             complement(6152..7210)
                     /gene="hisC"
                     /locus_tag="O0286_000420"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCY0168819.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(7450..8964)
                     /locus_tag="O0286_000421"
     CDS             complement(7450..8964)
                     /locus_tag="O0286_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCY0168820.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(8957..9934)
                     /locus_tag="O0286_000422"
     CDS             complement(8957..9934)
                     /locus_tag="O0286_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0168821.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(10156..11937)
                     /gene="recQ"
                     /locus_tag="O0286_000423"
     CDS             complement(10156..11937)
                     /gene="recQ"
                     /locus_tag="O0286_000423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCY0168822.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(11949..13832)
                     /locus_tag="O0286_000424"
     CDS             complement(11949..13832)
                     /locus_tag="O0286_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCY0168823.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(14103..16043)
                     /gene="ltaS"
                     /locus_tag="O0286_000425"
     CDS             complement(14103..16043)
                     /gene="ltaS"
                     /locus_tag="O0286_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCY0168824.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            complement(16517..17521)
                     /locus_tag="O0286_000426"
     CDS             complement(16517..17521)
                     /locus_tag="O0286_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0168825.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(17505..18215)
                     /locus_tag="O0286_000427"
     CDS             complement(17505..18215)
                     /locus_tag="O0286_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCY0168826.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(18303..18509)
                     /locus_tag="O0286_000428"
     CDS             complement(18303..18509)
                     /locus_tag="O0286_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168827.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(18575..19183)
                     /locus_tag="O0286_000429"
     CDS             complement(18575..19183)
                     /locus_tag="O0286_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCY0168828.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(19183..20334)
                     /locus_tag="O0286_000430"
     CDS             complement(19183..20334)
                     /locus_tag="O0286_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCY0168829.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(20318..20911)
                     /locus_tag="O0286_000431"
     CDS             complement(20318..20911)
                     /locus_tag="O0286_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0168830.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            21262..21930
                     /gene="queC"
                     /locus_tag="O0286_000432"
     CDS             21262..21930
                     /gene="queC"
                     /locus_tag="O0286_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCY0168831.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            21932..22351
                     /gene="queD"
                     /locus_tag="O0286_000433"
     CDS             21932..22351
                     /gene="queD"
                     /locus_tag="O0286_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCY0168832.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            22355..23068
                     /gene="queE"
                     /locus_tag="O0286_000434"
     CDS             22355..23068
                     /gene="queE"
                     /locus_tag="O0286_000434"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCY0168833.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            23167..23751
                     /locus_tag="O0286_000435"
     CDS             23167..23751
                     /locus_tag="O0286_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168834.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            24031..24471
                     /locus_tag="O0286_000436"
     CDS             24031..24471
                     /locus_tag="O0286_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCY0168835.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            24813..25286
                     /locus_tag="O0286_000437"
     CDS             24813..25286
                     /locus_tag="O0286_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0168836.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            25261..25947
                     /locus_tag="O0286_000438"
     CDS             25261..25947
                     /locus_tag="O0286_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0168837.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            25947..27002
                     /gene="saeS"
                     /locus_tag="O0286_000439"
     CDS             25947..27002
                     /gene="saeS"
                     /locus_tag="O0286_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCY0168838.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(27074..28057)
                     /gene="csbB"
                     /locus_tag="O0286_000440"
     CDS             complement(27074..28057)
                     /gene="csbB"
                     /locus_tag="O0286_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCY0168839.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(28189..29028)
                     /locus_tag="O0286_000441"
     CDS             complement(28189..29028)
                     /locus_tag="O0286_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0168840.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(29250..30599)
                     /locus_tag="O0286_000442"
     CDS             complement(29250..30599)
                     /locus_tag="O0286_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0168841.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(30820..32001)
                     /gene="nagA"
                     /locus_tag="O0286_000443"
     CDS             complement(30820..32001)
                     /gene="nagA"
                     /locus_tag="O0286_000443"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCY0168842.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
BASE COUNT        10480 a         5612 c         4592 g        11499 t
ORIGIN      
        1 gcttattagc ttgatactga attgatattt gttttatgta ataacacaaa cattcataaa
       61 agtgtaatgc cctattattc cgacaaataa acacagaacg tttgttcgct acagtctatt
      121 atagtctttt tatagtaaat ttcaactatt tatgcacaac atgttgtttt atgctaatat
      181 aaatacgtta attttatata aaagaggcga taaaattgaa tcctaaagta aaaggtatca
      241 ttgccatttt aatttcggct attggtttca gttttatgtc agtgtttttc agacttgcag
      301 gtgaccttcc tgtttttcaa aaatcattag cacgaaactt agtagcaatg tttattccat
      361 tattttttat atataaatac catcagccaa tgtttggcag actttcaagt caacccttat
      421 taataacacg atcaacactt ggacttattg gtgtattgtt aaatatttat gccattgatc
      481 atatggtact tagtgatgcc gattcattaa tgaaactcaa tcctttctgg acgattttat
      541 taagcattgt tttcttacat gaaaaggtaa ggaagtatca aattacagca atgataattg
      601 ctattttagg tatgctttta atagtgaaac ctgaattttc atcatctatg attccatcat
      661 tagctggctt attctcaggt atttttgcag catcagcata tacatgtgtt cgagcactta
      721 gtacaagaga agcaccatat actatagtat tttacttctc cttattttct gtcatcgttt
      781 taataccttt cactgcatat acttatgaac caatgagtca gatgcaaata ctttatctat
      841 taggggctgg cttagctgcc gctgtgggtc aaattggtgt tactttagca tatagttttg
      901 cagcagcaaa agatatttct atttttacgt atgcatctat aattttcaca gcaatacttg
      961 gatttattct attcggagaa tctccagatt tctatgcaac attaggttac gtcgttatta
     1021 tcggtgcaag ttattacatg tttgaaaaag cacgccgaga cgccaaaata ataaaaaaat
     1081 aaatttaaag gagcgcttta tatggcacat ggccgtcaac aagatgaatt acaagatata
     1141 actttattag gaaatcaaga caatacttac aattttgact atcgtcctga tgtattagaa
     1201 tcgtttgata ataagcatca aggtcgtgac tatttcgtaa aattcaactg tccagaattt
     1261 acatcattat gtccaattac tgggcaacca gattttgcaa ctatctatat ttcatatatt
     1321 ccaaatgtta aaatggttga atcaaaatct ttgaaattat acttatttag tttcagaaat
     1381 cacggtgatt ttcacgaaga ttgtatgaat attattatga atgatttgat agagcttatg
     1441 gacccacatt atattgaagt ctggggcaag ttcacaccac gtggtggaat ttctattgat
     1501 ccttatacaa actatggacg tccaaattct aaatatgaaa aaatggctga gcatcgtttg
     1561 atgaatcatg atttatatcc cgaaaaaata gataatcgtt aaatgtatca tttaataaac
     1621 acaccaataa gttgattttc ctaacttatt ggtgtgtttt tcatttagca tacataatag
     1681 gttacattaa aataacattt tataccaaag tacaccaaaa gaatattagt acacgaatta
     1741 aacaacattt ttatagaaac ctattgcact ttaacgtcaa taagtatatt tttatattat
     1801 ctctaattaa ttgtgcgcgc ttaataacag aatattctca atatttttat ttttttgtga
     1861 tttgttggaa tatttagttg ataaggcaca atcaaattta cttaaactat tgtattaggg
     1921 gaagaaagga tgggatgtat acatgacaca acaaaactcc catggaaatc aaattcaaga
     1981 catacctcaa acaggatttt tcgggcatcc tcgaggacta ggcgtactct tctttgtaga
     2041 gttctgggaa aggtttagtt attatggcat gcgtgcccta ctcattttct acatgtactt
     2101 tgccgtaaca gataatggcc ttggaattga taaaacaaca gctatgtcaa ttatgtcagt
     2161 ttatggttca ttaatctata tgacatcaat accaggcgga tggattgctg acagaattac
     2221 aggcactaga ggcgctactt tattaggtgc agtctttatt attatcggac atatttgttt
     2281 aagcttacca tttgcattaa tcggcttatt cacatcaatg ttcttcatca ttattggttc
     2341 aggtttaatg aagccaaaca tttcaaatat cgttggccgt ttatatcctg aaaatgatag
     2401 acgtatggat gcaggttttg ttattttcta tatgtcagtt aatatgggtg cattattatc
     2461 acctattatt ttgcaacact ttgttaatgt taaaaacttc cacggcggat tcttgattgc
     2521 agcagttggt atggcattag gtttagtatg gtatgtactt ttcaaccgca aaaacttagg
     2581 tagcgttggt atgaaaccta ctaacccatt gacaccagct gaaaagaaaa agtatggtct
     2641 tattatcgga agtgttgttt tagcaattgt attaattatc gttattggtg cattaactaa
     2701 ctcattatca tttaacttag ttagtaatac tgttttagta ttaggtattg cattaccaat
     2761 catttacttc actttaatta ttagaagtaa agatgtcact gatactgaac gttctcgtgt
     2821 taaagcattt attccattat ttattcttgg aatggtgttc tgggctattc aagaacaagg
     2881 gtctaatgta ttaaacatat atggaattga acattcagat atgaaattaa acttatttgg
     2941 ttggaaaaca aactttggtg aagcgatctt ccaatcaatt aacccattat ttattttatt
     3001 actagctcca attatttcac ttttatggca aaagcttgga actaaacaac ctagcctgcc
     3061 agtaaaattt gcaattggta cgttcttagc aggtgcgtca tacatactaa ttggtattgt
     3121 aggttatgca tcaggttcat caaacttttc agttaactgg gttattttat cgtacattat
     3181 ttgtgttatc ggtgagctat gcttatcacc aacgggtaat agtgctgctg ttaagttagc
     3241 acctaaagca tttaatgccc aaatgatgag tatttggtac ttaactaacg cttctgcaca
     3301 agcaatcaat ggtactttag ttaagttaat agaaccacta ggtcaaacaa attactttat
     3361 tttcctaggt gttgttgcaa ttattgttac aacaattgta ttagcaatct cacctttaat
     3421 catcaaagcg atgaaaggta tacgttaata ttgttggcct aattcaaaaa acagtaagtc
     3481 atttaaatgg cttactgttt ttttataggt ttctattaat taaattcaag atatcagtat
     3541 aaataaaaac ttaatatgct cattatagac agctataact atattttctc gtcccactct
     3601 atagaaatca atttcattta ttaccatttt ctctatttaa aaacatattt ttggtattaa
     3661 caattaaaag tgggtatata tatttaatga accaattttt agaaagtagg ctaaactatg
     3721 gaaaataaat atacacatgg tgtcctcttt taccatgaac acagtggatt aaaaaatatt
     3781 aatcaaggta ttggggaagt tacaacagca ttaagttcaa tttgtaagca tctttctatt
     3841 caattgagtg aaaacgaagg tgacattatt aaatattgcc aagaaatcaa aactaaaaac
     3901 tatgcaaaag atgtagatat attatttatt cttggcggtg atggcacggt taacgaactt
     3961 ataaacggtg ttatgacaca tgaccttcaa ctccctattg gcattttacc aggcggtact
     4021 tttaatgatt ttacaaaaac gctaaatata gcacctaatc ataaacaagc tagtgaacaa
     4081 atgatttctg cacaagttgg tacatacgat gtaattaaaa taaataatca atatgcactc
     4141 aactttgttg gcttaggtct cattgttcaa aacgctgaaa acgtacaaga tggttcaaaa
     4201 gatatatttg gtaaattgag ttatattgga tcaactgtca aaacactgct aaatccaaca
     4261 caatttaatt atcaattatc aatagatgat aagacatatt ctggcgaaac gaccatgata
     4321 ctaacagcaa atggtccatt cattggtggt agtcgtatac cattgactga tttgtctcct
     4381 caagatggtg aactgaacac tttcattttt aatgaacaaa gtttcagtat tttaaatgat
     4441 atttttaaaa agcgtgatag catgaattgg aatgaaatta ctcaaggtat tgagcatata
     4501 cctggtaaaa agatttcact tacaacagat ccagcaatga aagttgatat tgatggcgaa
     4561 atttcattag aaacacctat tgatattgag gttataccaa atgccattca gttacttact
     4621 gtcaatgatt tgtaagcatc cggaatgatc ttaacccgat attatattag gtttggagtg
     4681 taatcataag attcaacgca ccttatattc gtttttatta gctttaagct attaccctgt
     4741 ttcaaaaaca gacatataat tttggcgcat gccactaaaa tgattgatgt tgtattttaa
     4801 atcatagtgg tttatgcgtc tttttcaaat tctataaaaa atcggatgac gtgtaatctg
     4861 ccatagatta acacattcat ccgatttata ataataagat aaactaacat ttattgagag
     4921 cgggacggaa atgataaaga acgactaatg attgattatg tagcgattct ttatcattag
     4981 tcacagctaa tgtgtactta aaaatatgaa tgcatgagtt acactcatgc ataagaaata
     5041 ctaatttcta aagaaaaagt atttctttat gttggggccc accccaactt gcattgtctg
     5101 tagaaattgg cgttccaatt tctctatgtt ggggccccgc agtcaactac tgccaatata
     5161 gtgttgtagt gtctaagaca taaaagttca tctcagtcac aattacttta tagcctatcc
     5221 atcatctttt ctacatgaaa tttttcaagt gatatatttt atttctcaat attatctaaa
     5281 aaatactgtt ctacatcttt ccagctattt acgcgttcaa atcgatcatc attaatatta
     5341 tgcactgctg taaacataat cggtgtacca gtaaaaattt caagctgtct aggattgtca
     5401 tctattaaat aatcagcttt aacgatgttt tttctaccac aaaaaacaaa atgctgagga
     5461 tctaaaaatg gaaagaactc tagtaaccat tcatatttat cactaaatga tgttggtaca
     5521 tccattgctg ctgtagcaat atatacatca taatgttcag ttaatttttt cacaacttct
     5581 tgtgcatacg gcatcacttt aagatgtctg aagaagcctg attctctcaa tacttctgta
     5641 attaatccat catgttcagg aataacatgt ttaagttttt gaccattcaa agcttccatt
     5701 ttaataccta aatccgctct aaaattgaca gcatcaatga tttctcctaa tgtatctgcc
     5761 aatacttcat ccatatcaat cgcgattgat ttacgggtca ttttaatctt ctcctcaaaa
     5821 tatcagtatt gattcagtat agcaaaaaag caattgatac aaaacaatat catctattgt
     5881 tgctttttac actgaatact ctcacctcat cattcacact gcattgagct tgagacataa
     5941 atcaatgttc aatgctctac aaagttatat tggcagtagt tgactgaacg aaaatgcgct
     6001 tgtaacaagc ttttttcaat tctagtcaac cttgccggcg gggccccaac aaagagaaat
     6061 tggattccca atttctacag acaatgcaag ttggggtggg acgacgaaat aaattttgcg
     6121 aaagtatcat ttctgtccca ctccccactt actattcgta tttaaagttt gataaaactt
     6181 ctaacatttt atcattttgt tctttaaaac caattgtaat tctaacacca gttggaaatg
     6241 gtcgcgtaat acaccctaca tttaaaagtg cttcataaag ttcatttacc cgctttgttt
     6301 ttacaaatat aaaattcgtt tgacttggca agaaatactc actttgaggt aattgataaa
     6361 atctttcgcg ttcaacacta tttttatgtg ttacctcttt taaatattgt tgatcttcaa
     6421 gtgctgcaac tgctgcgtat tcagatatac gtgtcacatt aaatggtggt ctaatgatgt
     6481 tccatttttc aatcgcatgt tcacttgcta ccacatatcc tacacgtaaa cccgctaatc
     6541 catacgcctt tgaaaatgta cgtaataaga aagcattgtc atatttttgt tgcaaagcaa
     6601 gtgtatccgg gtagtcctct gctgtcacaa attcaaaata agcttcatct ataattactg
     6661 gtacatgtgg aggtacttga gataaaaacg aatctaagct ctcatgatta aaatatgtac
     6721 ctgtaggatt atttggatta cataaccata ccaatgacgt atcttcatta acttctttta
     6781 aaataccttc taaatcgaag ccaccatctt ttaaaggtac ttgtatcaca ttagctgatt
     6841 caacaatcgc attgtgataa tattgaccga atgtcgcttc acttgtaaca atagtatccc
     6901 ctggcgttaa tacagctcta gaaatcatta atataacttc atctaatccc gcaccaaata
     6961 aaatgcgtga ttgatctaca tttaaatgtt tactaatcgc cgcttttaat gtcggtgatc
     7021 ctgtttcagg ataataatat aactcatcta agtgtgctga tatcgcttct ttaactttag
     7081 gcgatggtcc atacaaattt tcatttgatg caagtttata taaatctcct tcaatgccat
     7141 acttttcttt caatgccctt ggagataaac caggctgata tgctgatagt tgattaagtt
     7201 gttctttcat aataaaaaca acctccttag aattaactga aatttcaaaa aacttatatt
     7261 cattatatcg ctctatgttt ctaaaatgca atgctactat tttcacatca taatagctta
     7321 cattgtgttt aacatgtcat attttttaat cattattgca tgtttaaatt gaacataatt
     7381 atagatttaa atataacaac gccacataag atataatttg gtgttacgca tcttatgtgg
     7441 cgttggtcgt tatttgatta acccttttgc ttttaaataa tctttcgcaa ctgtatatgg
     7501 gtcttcattt ttaactgtta ctttatagtt catcatttgc atatcttcat cagatatttt
     7561 gttttctagt ttgtttaacg gtttcttaat ttctggatgt ttctttaaaa agctttcttt
     7621 aaataatggt gctccttgat atggtggaaa tacgtgctta tcatctttta acacaaccat
     7681 atcatattgt tttaattcag cgtcagttga atatgcatct attaaattaa tattaccttt
     7741 attgatcgct tgatatctca acttaggttc cattgtacgt atgttatcta aatttaaatt
     7801 ataagccttt tgaacagctt tgtaaccatc tggacgatca ttaaattcca atgtaaatcc
     7861 tggtttaagt tgatctttaa ccttatttaa atcaccaatt gtacgtatat tatgttgttt
     7921 agcaaaatca cgttttacag ctaaagcata cgtattgtta tacttcattg gttttaacat
     7981 agtcatttga tatttctttt caagactttg cttagcttgt tcataaactt ttttctcttc
     8041 ttttgacttc aatggttctt ttgttaattc acctaaaact gttccagtaa attctaaata
     8101 cccatctata tcgtcagatt ttaaagcatt aaataaaaat gctgttttgc ccataccatc
     8161 tttcacttct acagtatttt tggtctcttc ttctattaaa attttataca tatttgtaat
     8221 aatcgatggc tcggagccaa gctttccagc taacgtaatt ttatcacctt tttgtgcaaa
     8281 cataggaata gcgatagcca gtataataat catcactata acacctaacg tcatcaataa
     8341 ctttttataa gataatttag ccataaatct taaaattaaa tcaaatataa ttgccaataa
     8401 ggctgctgga attgcaccta ataatatcaa cgatgcattg ttacggtcta tacctaataa
     8461 aattaaatct cctagtccgc ctgcaccaat taatgctgct agtgttgctg tacctataat
     8521 taataccata gccgttctta caccagccat tataacaggc attgctatcg gaagttcgac
     8581 tttagttaaa cgtctaaatg gtttcatacc tataccttta gccgcttcaa tgagtgatgg
     8641 atcaacttct ttaattccag tatacgtatt ccttaaaatt ggtaacaacg catacactac
     8701 aagtgcaata attgctggca cacgaccgat accaaataaa ggaatcatta aacctaataa
     8761 tgccaacgat ggtatggttt gaagaattgc cgcaatattc attacgattt cagatatcgt
     8821 tttagtcttc gttaataaaa tacctaatgg taccgcaata gcagttgcaa tcaataatgc
     8881 gataaatgat atttgaatat gttctatcat tgtcgaaaag agttgcccct tacgttcact
     8941 caatatgtcg aaaaagttag tcatgttgag ctacctcctt tttctgggac aaatatttga
     9001 agatatcttt cctatcaata acatattgac ctacgctatc ttcttgcatg acaatgacac
     9061 gctcgctctc tgataaaagt tgatacaata cttcaattgg ttgattgtca taaacaattg
     9121 gataagcgct catagatgta acctcatcga ttggtttcat aatatccaag tcacggataa
     9181 ttgcgttctc ttcaacacat ggcgcatcat cttctaaatg actacccata aattgtttaa
     9241 caaattcact ttgaggatta tttttaaatc cttctggtgt gtcaatttgt tcaatatgcc
     9301 cttcattcaa aagacaaatc ttatcaccaa gtttcatcgc ctcttgaata tcatgtgtaa
     9361 caaatatgat tgtcttctta attttagttt gtaattcaat taaatcatct tgaagttttt
     9421 ctcggctgat tgggtctaat gcactaaacg gttcatccat taaaataact ggtggatcag
     9481 ctgctaacgc acgtataact cctacacgtt gtcgttgccc ccctgacaat tcatcaggtt
     9541 ttctgttttt atatttttca ggttctaatc caaccatttc aagtaattca tctactcttt
     9601 tatctatatc tttttctttc cactttttca tttgtggcac ttgtgcaata ttttctttga
     9661 ttgtcatatg tgggaataat gcaatctgct gcaatacgta tccaatatcc caacgcattt
     9721 cgtatactgg ataatcactt attggtttat ctttaaaata aatataacct tcacttaagt
     9781 gaatgagtcg attaatcatt tttaatgtcg tagtttttcc acaacctgaa ggtccaatta
     9841 gcacaaaaaa ttcaccctca ttaatattga aactaatgtt atcgacagca acatgtttgc
     9901 cataacgctt agttacattt ttaaacttaa tcactttgcc acctcttttt ttctcatagc
     9961 ataaaaccga gattatatgt atgtattccc tatttaacca cgtttattac aattttcaaa
    10021 tttaaatgat ttatccttga acttttttaa taaaataatg aataataggt aatctccagt
    10081 taagaaatag tgttatttta ccttgaattc aaaaaaacac ccagtaaaac aaggaatgct
    10141 tactaggtgt cttcactata ctttggcttt ataattttga atcgtttcta aaaatgctgg
    10201 acaataatgt tttaatttgt aactacctac gccatcaata ttaatcatat cttgtttcga
    10261 agcaggctta cgtttagcaa attcctccaa cgtgtaatca gaaaatatac ttacaggtgc
    10321 tatcgttaat ttgtcactta actttttacg aacttctacc aactgactga ataatactcg
    10381 gtcaacccct tcaaccgtat ttataaatac tttttcagtc gctttttgct taaatggtgt
    10441 tgtgaatact tctacttcat tactgagtaa ttttttaatt gaagtatcac acattaatat
    10501 ttcgtcattt tcatttaaga accctttgaa tcttaattca tctattaagt gacttaattc
    10561 tgatgttgtg taacctttca ttaaaccatg ggttgaaatt tggtcataac ctttatactt
    10621 aatataatct gttgactctc ctcttaacac ttgaatgata acactataac tctcttgttg
    10681 tttcatacga gcgatgcaac taataatcat cttagcttct tgtgtcatat tatatgattt
    10741 atcttgttga acacaattac tacattgttc acattcttct aatttttcat tcggttcaaa
    10801 ataatggaca attgttgctt ctagacattt ttttgttttt gtatattgaa tcattttagt
    10861 taacttttcg cccattttat ctttatagtc atcatcagct tgagagactg ttataaaata
    10921 ctcgtgtaaa ttgatatcgc gttcgctaaa taacaaaata cattcacttt tcaacccgtc
    10981 acgacctgca cgacccgctt cttgataata agattctaaa tctccaggca tattataatg
    11041 aataacaaag cgtacattgg atttatcaat acccatacca aaagcatttg tagcaacgac
    11101 tactttaaca cgatcaaata agaaatcatt ctgcgcttct tctctttctt tattgctcaa
    11161 acctgcatga tatataacac tttcaatttt ctgactttct aaggcttctt gaagctcttc
    11221 aacttgctta cgtgtagaac aataaataat acctgcatct tcatcgtgtg ttttaatata
    11281 atccaatata aatttttgac gttgataagt aggatttact ttaaaaatta agtttctacg
    11341 cttagtactc gttttaattt gatcagtttg agcgatattt aacttttctc taatatcttg
    11401 ctgtacttca accgtggcag ttgctgtcaa cgctattatt gtaaaatctt gaggtaacgt
    11461 aaatactttt gaaataacat tttggtaact cggcctgaaa tcatgacccc atttagaaat
    11521 acaatgcgct tcatcaaacg cgactaagtg aatctttata cgctgaagca tatttaaaaa
    11581 atatcggttt tcaaatcgtt ctggtgcaac atacaaaaat tgaatttctc catttgataa
    11641 tgctttttca atacgttgtt gctctttttg agtcaaacta ctatttaaaa aagcagcttg
    11701 aattcccatc gcttttaatt gatccacttg atctttcatt aatgatatta gtggacttat
    11761 tacaattgtt gtaccaccta acaataaacc tggtacttga tagcatatag acttacctcc
    11821 accagttggt aagacaccaa gcacattacg atggtctaat actttgctaa taatttcttc
    11881 ttgtcctggt cgaaacgttt catacccaaa gtaatgcgat aatgtttgtt gcatcataaa
    11941 ttgacccctt attgttgttc tttaatttct tctaactcac tccatcttgt gatgtctaaa
    12001 tcatattgaa tttcaagttg ttctttttct tcgtttaatt ctttaatttt cccataatct
    12061 gcacttgcct caatcatgag cacatcaatt tcttccattc ttacttccgc ttgttctatg
    12121 cgtttcatca attgttcata ttctaatttt tctttatatg ataaaccatt tttcttacgt
    12181 acagttgtag aagatttaga ttgttgcttc aatgtggatt tatttttatc taatgatttt
    12241 ttataacttt cataatcttc aaaagttccg ataatctttt ccatctgacc atcatgaata
    12301 aaccaatatg actgtgcaac tttatttaag aagtagcgat catggcttac ggtaatcact
    12361 gtaccaccga aagtatgaat ataatcttca agtattgtta aagtctcagt atctaaatca
    12421 tttgtcggtt catctaacaa cagaacattt ggctggtgta cgagtagacg taataaatac
    12481 aaacgctttt gctctccacc agataattta taaacttttt taccatgagt tgcacttgga
    12541 aataaaaatc gttcaagaag ttgtgtaata gataccgagg ttccatcttt ttcttttgcg
    12601 atctcatttt cttctcttaa ataatcaatc atacgaatat ctctatccag ggtctcatct
    12661 gtttgcttaa aataagctac tttaaccgtc tgcccagtct tcaatttacc ttcgaattgt
    12721 tggtcttctc cactcaaaat atttaagagt gttgttttac cagctccatt tggcccaaca
    12781 acaccaatac gctcaccttt ttgaataatt tccgtcagat gttcaattaa tactttatca
    12841 ttaatagcct ttgataagtc ttctaattcg aacacttgct tacctaatct tgaataagca
    12901 agattcaatt cacctttatc gtctttatat tgctggttaa cttcattttc taggtcatta
    12961 aatcgattaa ttctagcttg ttgctttgta gtacgagcct tagctccagc cctcatccaa
    13021 gcaagttctt ccttatataa agctcgttgc ttttgttgtt gcttttgaag tgttacttct
    13081 ctttcagcgc gcatttcaat ataagattca tagttaccag gatatgacgc taacttacct
    13141 ctgtttagtt caataattct agtggaaact tcatttaaaa aatatcgatc atgggttacg
    13201 aataaaacag tatgaggata ttgcttcaca taattgatca accagctgat tgattcgaag
    13261 tctaaatggt tcgtaggttc atctaataac aataaatctg gttgttctat taatgtttta
    13321 gcaagtacaa cacgtttttg ttgtccgccg gataattctt taatgtattt agtagtatca
    13381 tgtataccta gttttgagag gattgtttta atttcagcgt tatagtccca agcatcatgt
    13441 tgatccatag catcttgcgc atccatcatt cgcttgaaca atttatcact ttggtcatcc
    13501 gcataagctt gtactgcctg ctcatattgc ttgatgatgc gtaaagttgt tgtatcagaa
    13561 cttaatactg catcaaaaac tgtcatatct tcattaaggt cctgtttctg agacgaatat
    13621 cgaattcgat attgatttgg atgcataaca ttggctgtaa aatcatcatc aataccacca
    13681 attactttta acaacgtact tttccctgta ccatttatgc ctactaaacc tattttttca
    13741 ccttctgaaa ttgataaatc taggttatcg aatatagtct tatcggcata agatttattt
    13801 aaatgttcaa ttttatatgc ttccataccc atcttccttc ttctgttgaa atctctacgt
    13861 acttaaatca gttctataca ttatacacct ttttgaaatc aaaagaaatc gataccgtta
    13921 agtgtcataa tttaatatat tgttgcttca actataaata aaacttgtta aggctttgat
    13981 gactatgcta atgatcttta taatatacct ttttatcaac acatataatg taaaacataa
    14041 tataaaagaa cataataaaa acccggattg atattttatc aatccgagtt cgtgtttaga
    14101 tattattttt tagagtttgc tttaggtcct gtttcatatt tatacttcga aggatttacc
    14161 tttttgaagt ctggattttt gtagaatcta aacaaatcac cattaagcac gttgtcactc
    14221 atttcgagat ccttttcaac ttgcttttta ttcttttcga aatcagctgg ttgagtagtt
    14281 atgagttcat tatttttatt agaataaatc ttaccattaa catatttata atcttttgtt
    14341 ataaagtcac catttctgaa tggaactact tgattatgac ctttagagaa taaatcagta
    14401 ccgaacatta aatagttctt cgtatctata ccagccaaat gtaaaattgt tggcattaca
    14461 tcgacttgac cagcatattc attattgata ccaccagatt taccagggat tttaatccag
    14521 aaaccagttc tgtttaaatc tgtaaattta gccggtgtga ttttttcacc taatagtttt
    14581 tccatggcat tgttatggtt ttcagagata ccatagtggt caccataaat cataatcact
    14641 gaattgtcat ataatccttt tttcttcaag tcattaatat attcttctaa tgcttcgtct
    14701 aaataacgtg ctgtttgaat ataaccatca actgttgcat cacctgtgtt tgatttctca
    14761 atagttgcat ccttttcatc taatgtgaat ggatagtggt tagtcaatgt aattaaatga
    14821 gaatagaatg gtgatttcat cttagcttga taattagcag aatctttaaa gaaaattttg
    14881 tctttcaagc ctaagtttac aacgttttta tctgacatgt catagtatgt tgcatcatag
    14941 aatttatcga taccaaagtg tttatatact tggtctctgt tccagaatgt tttatagtca
    15001 ccgtgcatga catcagattt gtagccttgc ttttgatcta aaattgctgg taatgactga
    15061 tacgtattat ctccttttaa tgaaaaggca gaaccttgcg gtaaaccgta taaactgtta
    15121 tccattgtaa attcagagtc agatgtttta ccttgacctg tttgatggaa aaagttaggg
    15181 aagtatgtga attgctcttt cccacttgat aatttgttta aaaacggtgt tacttcttta
    15241 ccattaacct ttttattaat taagaaggtt tggaaacttt ctaaatgaat cttaataata
    15301 tttttcttct ttgccacccc ataatattct gggttaggct ctgtttgacg ttgtttcgta
    15361 taatttaata cttttgttaa gtcatcttca gatgctagcg ctttttgttg attattttcg
    15421 atagttttaa caccatcgta tactgtaaag ttataaggtc ctaaatattt cactaaatat
    15481 ttatggtcaa atgtacgtgt taataattct ggtctgtcag tttcagcaaa agctaagttt
    15541 aagaagaata atgctactga agccgccatt acgacaggaa caaatttctt actaaatgct
    15601 tttgtgtcta accattttgt tttaaatatt aaaatgaata agtaaactaa cgtatcaatg
    15661 aaataaacaa agtcatacca tttgaatgat gcactaaccg caccacccat agattctacg
    15721 ttacctactt ggtttaaagt actaaacgtt aaaaaatcag agaagaatct aaagtacaca
    15781 acattggcat ataataggaa cgtcaataag aagccgccta tgaacatgaa ccaaaatgct
    15841 tttttgcctt taaagaatag gaacacactt aaaaccagtg ctactaaact ataaggattc
    15901 atcaataata ttaagttttg tactaaacct ttaacaccta aagaaaaatc aacataataa
    15961 gaaaaatacg tcttcaaggt aatcgttatt acggttaata agaagaacgc aaaaagacta
    16021 attttctttt tttgtgaact catgattctt tcccccgtta tttagataat aaatcttgcg
    16081 aaagtacttg aataaattag atattaacat tatgtagtta attaataagt gcgtttaaaa
    16141 gttaacattg tcataattat agtaattact ttcagattca agtttataat tttcgttatt
    16201 tgtagctttc aactttgatt taacaagtat tttgggctac aactcatatt tgcttgtgta
    16261 agtttcttta tttaatatta tcattcaatt aacaaattta aaatttgtaa tcaaattgta
    16321 ataataagca tagctcaatt aataaatata gagtaatttt agtcaaaatt caagtaaaaa
    16381 cacactttat cttttcattt tactgtgaaa cgaattagaa aatgatacat ttcgcagata
    16441 aaacacagta ttatttcatt atacattagg aggaaattat ttgcatcgga ctcgagtatg
    16501 agctacgtac caaatattat ttcatcattt ctaattgcat tctatattct attccatgga
    16561 ataattggtt taaccagtta ctatacttaa caaggttctt ttcagccgtt aaaatatcta
    16621 taaattgaaa tttcaaacgt gatccttgcg gtttttgtgc taatttcgtt aaatgataac
    16681 ttgcgattgt accgatttgc gggtagctac ctagcgtata atgatcattt aataggataa
    16741 taggtgtgcc atcacgctta atttgtatcg ttcctttttt gacagtctga tacggtggca
    16801 tatcttcata aaaagctttg attttttcac cttcaagcat catccccatg cgatttgctt
    16861 tgctcgttac tttataatca tgtttcacaa atctttgaat ggcatcttct ttaaaatctt
    16921 ccgtaccttt atttttaacg acatgaaata catcagacat ataattaaat gacaaggcgt
    16981 atccatcaat accccaatct gtttgtttcg tgtgagcaag gttttcaaat aacttatgat
    17041 gacgagctgt ataatctctc ttaagcttta tttcatcgcc atcttgtaat gttctacctt
    17101 taaaaccacc aatttttaca ttaaagtcgg ttgagttaga tcctaaccat gcatctaatt
    17161 caaatccgcc tcccacagct aaatacactc gcgatgtata acttgtttct ctaaacttta
    17221 aaacatcgcc tttatctaac aaatacaatt tatatggaga tatagtcata tgctcagtgt
    17281 aagctttgac attaccccct gctaatgcaa tcagcgtagg ttctgtaaaa cgaatcgttg
    17341 ccattttatt agtcatttcc aaagttgctt cattcttgtc atttgcaact aatcgattag
    17401 caatttcatg tgctaaagta tcaagtgcac cacatggaat tacaccatca tgttcatatc
    17461 cccttctgcc aaagtcctga aagctactga ataagccact tttttcaatt atgattgaca
    17521 tctttaaacg ctcctacttc taattcattt tcattgatag gtctaaattt aatattatcg
    17581 cctaacttta agcgtgcgaa atctgattct ttcggattaa aaagtgataa tggtgtataa
    17641 ccaataacta accaatcatt aattgtatcc gtagttacaa taccgcattt tttcccttca
    17701 agtactacag aaccagctgg aatgaatttt ttctgtttac tcgtgtgatt aatatacaat
    17761 ttcttattta atccagttaa ataaggaaac ccaggtgaat atcccatcat cgaaacaaaa
    17821 tattgcgcct tagaatgtaa ttcaataaaa gtttctagct tgattttgta atgttttaaa
    17881 agtgattcta aatccggacc atatttcgaa ccatacacaa ttggtatttc aatcaaatct
    17941 tgctggttaa aatcatgttt tatgtttaaa tgaatcgatt ctattagtgc tttcatgtat
    18001 aaaaaaggtg attgtatatt atggtgttta atcatatccc ttgcgtcata gacaatcatc
    18061 atgtctgatt ctgatggcac aatttctata ataaatggat aattttgttc aattaaatat
    18121 gagcgaagtg ttaataaatc ttcggttaaa cttttagata cttctttttc aattgcgact
    18181 acaatggctt ggtcaccttg actatatatt ttcatttgta cacctcttca atatttggtt
    18241 actttacttt catttttaat tatagaatcg attgaatagt tcattttacc tactcactaa
    18301 atttactggt ggtacattaa atccattgcc cgcacaacaa aaggcataag ttcgtgaatt
    18361 aatgcgtata caaggataaa gcttataaca gtagtaattg ttgctatcaa acgaacaaca
    18421 tatattctat tttcagatag caatcgattc actaatcgat aatttacgta tacaagaatt
    18481 aacaacagca caatataaac aataatcata tttggcccca tttaattttt aaatttgttt
    18541 tcacatcatt ttctacttta ttatcatact aattttaaag gcaaaggtgg acatcggcaa
    18601 cctctcgtaa actatttatc aaaaataaac gtatctcatt gttatgatat ttataaatca
    18661 attcgttttt attaaagtct ttttcaacaa gtttgtcact atctattaaa taatcacgca
    18721 tgcatccttt taagaaatca tctttataac ttggtgtgta ccattttcca tcctcttcaa
    18781 tgacaatgtt gccaatatca aattcaagga ccttgccgtc ctctgaagtt agcaatatta
    18841 aatctgtttc atgattgtgt gctaaatgct ttcgttctgt cgttttattt tttataaaag
    18901 ttggattaac tactttgggc aacacttgaa attttgctgt aaaactactt ttctcaggta
    18961 aaggtgacac tatcgtttca aatttgcttt cagcatttaa tacgatttta agtctatatt
    19021 gaccactgtg atactttgtt gttacatcgt ttaattcatt tcgccatgca tgttcatcaa
    19081 atttaaagtt taatcgctca gaagaacatt ttatgcgatt agtatgataa gtaagtctag
    19141 ggatatgtcc attatcaatt ttcattgttt caaataattg cattataaca tctccaaaat
    19201 cttggttttt gcgtaaaatt cattcacttc atcttttggc ttagaatcaa ttgtaatacc
    19261 tgctccgact ccataaatcg cttgtccata tttatactca atagtgcgaa tcggaatatt
    19321 aaaaatcatt ttatcatctt cagttggaag taatagtcca attgctccgc agtatatacc
    19381 acgaggtgaa ctttctaatt gtttaatata tttcattgta ttcagtttcg gtgcacctgt
    19441 aatcgaacca caaggaaata acgatgttaa aatttgagtt aagtctgtat tattttttaa
    19501 agttccactt accatcgaag tcatttgaaa tacagtttta tatgcctcaa taaaaaatag
    19561 tttatacacc ttaattgagc cactctgtga tattctccct atatcgtttc ttagtaaatc
    19621 aacaatcatg acattttctg cacgatcttt cgaagaagtt tgcaatgttt tataatactg
    19681 ttgatcttca gcttccgttt tacctctagg cattgtccct ttcatcggtt tgcttatgat
    19741 aacgttatcg acattgttaa attgtccttt ttgaaaaaat aattctggtg agatagacgc
    19801 tacttggatt tcatcagttt gtaataacgc agtataatta ccattactaa attgagttaa
    19861 tcgttcatat aaagtactaa taggataata aatgttatcc gttaagcgcg ccgtatagtt
    19921 cacttgatac gtttcgcctt caacaattgc ttgttggaca cgtttaatat tagtcatcat
    19981 aacctcagaa gattcaacaa atgaaaaatg atactttgat acataagaag tttgatgttc
    20041 atatgttgaa tttatgcttt ccgctttttc aaaactataa gctgctgcat aaatatcatc
    20101 tttagctaat gaatgtgtac acatagcatg attaaaatac tttgccgctt cgtaacttaa
    20161 atataacgag acatatctac cttgtcgttg ctgcgcttgt gcaaagtgta tcacttctcc
    20221 cacatcagcc aacttagtag caacatactt ctttataaat ccctttaatt gaatatggta
    20281 ttgcttatat tcattttcag ttaaatagta gcgataatta tattctattc tcacagtaat
    20341 cacctacctt cgataaaaat aattcaactt gtcgataacc gtattcactc aaaatagatt
    20401 caggatgata ttgcacacca aaaaccggaa atctaatatg ctcaaatgcc ataataatcg
    20461 cttcatcgtt ttttgctgta atctttaagc aatttggaaa agtcgctccg tcagcaatta
    20521 atgaatgata acgcattaca ttgaaatttt gaggcagtcc ttgaaaaata ccttcattgg
    20581 tatggcgtaa ctgtgtagta tgtccgtgta caggatgata gccgtgaatg atatttccac
    20641 caaaataaga cacgatacat tgaaatccta aacatacacc tagtataggt acacgctgat
    20701 aaaattgttc taacacttca ttcaagatag gataatcatc cggattaccc ggcccaggcg
    20761 aaataacaat tgcttttggc ttcatattaa tgacgtcttc tatcagcaga ttatcaatac
    20821 caacaacttg aactgttagt ttcgtttgag tcttaatata gtctattaaa ttatatgtaa
    20881 atgaatcatt attatctatg actagaatca ttgtatactc cgttctaaat gtgttttatt
    20941 tttataatat gtattggatg tagctaaaac tttaaaagca ttgtcattat cctgaccttg
    21001 attttaacta atatatggta tattctattc atcgtacata aatgaatatc agaggttcct
    21061 agctgaaacc ctctataaaa aactagacat tgaaatttca aacattcgtt ggtaaacctt
    21121 taatatatat tataggagca acccatttct ttcgtctatc ttttatggag ataggctttt
    21181 tttattttat aaattaaaaa cgggcttatc aatgccatta acctctttat taatattatt
    21241 aatgatagga gcaattacaa aatggaaagt gtattaaata atgaaaaagc catcgttgtc
    21301 tttagtggtg gtcaagacag tacaacatgt ctcttttatg caaaaaaaca tttcaaagaa
    21361 gttgaactcg taacgtttaa ttatggccaa agacatgata ctgaaattga agtcgcaaaa
    21421 caaattgcac aagatcaagg aatgaaacat cacgttttag atatgtcatt attatcacaa
    21481 cttactccaa acgcattaac acaacatgat atggaaatta ctaataatga agatggtata
    21541 cctaatacat ttgttccagc tagaaattta cttttcttgt cgtttgcagg cgctctagct
    21601 tatcaaattg gggctaagca tattattaca ggcgtatgtg aaacagactt ttcaggctac
    21661 ccagactgtc gcgatagttt tataaaatca atgaacgtaa cattaagcct agctatggac
    21721 aaagattttg tcattcatac tcctttaatg tggttaaaca aagcagaaac gtggaaatta
    21781 agtgatgaac tcgaagtttt agattatatt cgtacaaaaa cattaacatg ctataacggt
    21841 atcattgggg atggctgtgg tgaatgtcca gcttgtcatt tacgtcaacg tggactaaat
    21901 caatatcttg aaagtaaagg agcgctttaa aatgttacaa caaatctatc ctagtacaac
    21961 gcatccatat caattcgaat taaataaaga ttttaatttt tcggctgcac atcacattcc
    22021 atgtgaagaa gcaggtattt gtcaaaatgt ccatggtcat acttactttg ttaatttaac
    22081 aattgtcggt gataaactag atgacactgg cttcttagtg aactttagcc atttgaaaaa
    22141 gatgatacac ggtaaatttg accatcaact gttaaataac ttacctgctt ttaaaaacaa
    22201 aatcccttca actgaaatcg tagcggaaac aatttatcaa attgttaaag aaaatttggc
    22261 atcgctcgaa caccaaccaa aatgtattca agtatttgta agagaaacac caacaagtta
    22321 tgtcgtattt agaccaaagg aacaggtgta actaatggct aaaattcctg tactcgaaat
    22381 attcggtcca accattcagg gtgaaggtcg agtaatcggt agaaaaacga tgtttgtgag
    22441 aacagctgga tgtgattatc gctgtagctg gtgtgattct gcatttactt gggatggtag
    22501 tgctaaaggc gatataaaac tcatgaccgc tgaagaaatt tatgatgaat taaaacgaat
    22561 tggtggcgat ttatttaacc acgttacaat ttctggtggt aacccagcat taattaaagg
    22621 tatccaagag ttagttgact tatttcaaga taaaggtatt ttcagcgcac tggagacaca
    22681 aggcagtaaa ttccaacctt ggatgacaca aattgatgat ttaacgatta gtccaaaacc
    22741 accaagttca actatgacac ctgatttaaa aaagttagat gaagtgatta cacaatgcgt
    22801 accttcatca ttaaacttaa aagtagttgt attcgacgat aaagattatg attttgccaa
    22861 aatgatacac caccgttatc cagatattcc cttctattta caagttggta acccatattt
    22921 atcagacagc gtagataatc ataccgaaaa gttgttagaa cgctacgagc agttggtgga
    22981 tttagttatg caaagtaatg atatgaatca cgtttatgtg ttaccacaac ttcatacatt
    23041 actttggagc aataaaaaag gtgtataaag gataacttat tttacttttt gcaagtcata
    23101 tctaatgaat aacaatatat taattgatat aatagttgat tgaagtataa cgaacaggag
    23161 tccatcatga ttgtatatat attaattaat attgctatcg tcgtacttat aactggattc
    23221 aatttatata ggcatcaaat gcaacattta tcattaagtg caatgttgtt atctattacg
    23281 ataaacgcct ttataaatac attcataatt gataaatata actttattac tctatgtact
    23341 ataacgatgt ttatcatttg gacgatacta cagttttaca ttgataaaaa attaaaacct
    23401 gtatacatta cagaccaaaa atttattgct atcatattaa cgattgttgt cagtttaacg
    23461 caacgtgtta ccgacttttc aagtacacaa tcaatatata tgtctattcc atttttagcc
    23521 cctgccattt tcatcattgg tggtattatg ttgtttattt caacatttaa tagcttagat
    23581 gaaactgctg aaaataataa caaaataaag aaactaatga ttaaaggact tatcattatt
    23641 aacatttcat ttatcgttat gatggtttta acaccatatt ggtacttgta tttaatcgtc
    23701 tatcttattt tcttgttgtt tttattgtgg caaaaggttt ataaatttta ataccaaaac
    23761 tattaaacac ttctgatatt cttagttcaa aatatcagaa gtgttttata gtgttatcta
    23821 gttcagataa atatttcctt acttaaaaaa acgccctcct cttattttga cccctattta
    23881 tttaaatcag acaattattt tcattttcaa attattcttt cttcaatatt agttaagcga
    23941 tatttaaacg aagttaagaa ttagttaatg gcatattatt tgccttcatt ttaaacttaa
    24001 cttatcaaat tgaagaaatg aggagttagc atgaatacaa aatatttttt agcagctggt
    24061 gctgttatca caacattagc tttaggtgct tgtggtaatt ctaattcaca agatcaaggt
    24121 aacaaaactg aacaaaaaac aaagtcagaa gatagcaatg ttaaaactga taaaacaaaa
    24181 cacctaacag gtacattcag ttctaaaaac ggtgaaactg ttgaaggtaa agctgagatt
    24241 aaaaatggta aattaatgct tactaactac aaatcatcaa aaggtccaga tttatacgtc
    24301 tacctaacaa aaaatggcga cattaaaaac ggtaaagaaa tcgcaatggt tgactacgat
    24361 aaagaaaaac aaacatttga tcttaaaaat gtagatttaa gcaaatatga tgaagtaaca
    24421 atctattgta aaaaagctca cgtcatcttc ggcggcgcta aattaaaata atcaatttct
    24481 gagttaaact tttatttaca acatactatt actatacatt acaaattttt aaaatataca
    24541 ttacactcat tactcaatgg aaagcgtatg atttcacagc ccccctagct tgtagaaatc
    24601 atactttcct tttttcaata tatatacaac tattaaatcc cataagattg cagagcacat
    24661 aagtaaattt ttttagagct tgaggtttgt ttagcttaag caacccatga gctcaaacac
    24721 ttcctgttca cataacacta caaatcgcat tatgttgctt aatcttatgt ttatataaat
    24781 tacacacaat aaatagaaag aatgtgaaca tcatgaataa attattgcta ctcgttacat
    24841 ttatcattcg tgtgggttca ggtattgtta tgttaatgca aggctacgaa aaattaacgg
    24901 gcggatttac gctgaaaggt ttagtaccag tcatcgctaa caatactgat tcaccagagt
    24961 ggtataagtg gtttttcgca aatatagttg cacatacgac gtcattattt gatattgttg
    25021 tcccactcgg agagattgca attggattag gtttaatttt tggagttttt gcatatgctg
    25081 ctagtttctt tggagccttt gttatgataa attatatctt agcagatatg atatttacgt
    25141 atcctcttca attaactttc tttatccttt tactaatgag tcactcattg ttaaaacaga
    25201 tttcacttaa agaaatcatt aattacttta gaggtcgtaa gaacagaggt gaaaaaatag
    25261 atgacccact tactgatcgt ggatgatgaa caagacattg tagacatttg tcaaacctat
    25321 tttgaatatg aaggttacaa agtaacaacg acaactagcg gtaaagaagc aatttcttta
    25381 ctatcaaatg atattgatat catggtactt gatatcatga tgccagaagt taatggttac
    25441 gacattgtca aagaaatgaa aaggcaaaaa ttagatatcc cctttatcta tttaactgcc
    25501 aaaacacaag aacatgatac catttacgcc ttaactttag gtgcagatga ctatgtcaaa
    25561 aaaccattta gtccaaggga actcgtttta cgtattaata atttacttac aagaatgaag
    25621 aaataccatc atcaaccagt tgaacaactg tcgtttgatg aattaacact tattaactta
    25681 agtaaagttg tgactgtaaa tggtcacgaa gtccctatgc gtattaagga atttgagtta
    25741 ttgtggtatt tagcttctag agaaaatgaa gttatttcta aatcagaatt acttgaaaaa
    25801 gtttggggat atgactatta cgaagatgct aataccgtga atgtccatat acaccgtatt
    25861 agagaaaaat tagaaaaaga gagctttaca acatatacca tcacaactgt atggggatta
    25921 ggatataaat ttgaaaggag ccgataatgg tgttatcaat tagaagtcaa atcattattg
    25981 gcgtcgtatc gagtatacta ttaacttcaa ctattttagc aattgcatat attttaatgt
    26041 ggtttaacgg ccatatgaca ctaactttga ccttaacgac aataattaca agctgtttaa
    26101 ccttattaat atgtagtatt tttattaatc cacttataca aaaaattaag cagtttaata
    26161 taaaaactaa gcaatttgct aacggaaatt acgcaagcaa tgataaaacg tttaattcac
    26221 caaaagaaat ttatgaatta aatcaatctt ttaataaaat ggcttctgaa attacgcaac
    26281 aaatgaatca aattaaatcc gaacaacaag aaaaaacaga actgattcaa aacttagccc
    26341 atgatttaaa aacaccttta gcaagcatta tttcatattc tgaaggacta cgtgatggta
    26401 taatcactaa ggatcatgag attaaagagt catacgacat attaattaaa caagcaaaca
    26461 gattatcaac attatttgat gatatgactc atattatcac tttaaataca ggtaaaacat
    26521 atcccccaga attaatacaa ctagaccaat tacttgtatc aatattgcaa ccatatgagc
    26581 aacgtatcaa acatgaaaac cgcacattag aagtgaattt ctgtaacgaa attgatgcat
    26641 tttatcaata tcgaacgcca cttgagcgta ttttaacaaa cttacttgat aatgcgctaa
    26701 aattttcaaa tgttggtagt cgcattgata ttaatattag tgaaaacgaa gatcaagata
    26761 ctatcgacat tgctattagc gatgaaggta ttggcattat accagaacta caagaacgta
    26821 tattcgaacg tacattcaga gtagaaaact ctcgtaatac aaaaacgggt ggttctggat
    26881 taggcttata tatagctaat gaactcgcgc aacaaaataa cgcaaaaatc agtgtaagca
    26941 gtgatataga tgtaggaact acgatgactg taacattaca caaattagac attacgtcat
    27001 aatccgattt atttataaaa taaaatgcaa agactaaaaa gaagctccca ttaatgaggg
    27061 cttctttttt tgtttattta gaataaactt tatgggtatc cttctcatca ttttcaagac
    27121 ttgaaagatt tgtagcttga ataatatatt taggacgtgc cttaacttca taatatatcc
    27181 tgccaatata ttcacctaca acaccaattg aaattaactg tatgccgcct aataataaaa
    27241 tagctgcaat cgttgaaaaa tatcccggaa tattaacacc agatatcata atattgatga
    27301 ataaatagat aatatataac acgcttattg aaaagataaa caagccaaga taaatcatca
    27361 ttctcaaagg tttactatta aaggaaatca atccatcaat accataatta aataactttt
    27421 taaaggacca cttagattcc cctttttgtc tctcaacatt ttgatacgta aacactttag
    27481 tattatagcc tatccattca aataaccctt ttgaaaatcg attatattct tcaagtgatg
    27541 caatggattt aacagctctt tggcttaaaa gtctaaaatc accaacacca tcatcaaatt
    27601 gtacttcttc tacaaagcaa ttaactaact tataatacaa atggcttaat gttttgcgac
    27661 taaaattttc accacttcta tcacgctttg caatcacttg atcatagcct tccataaaac
    27721 cttcaaccat tttcggaata tattcaggtg gatgttgcaa atcacaatct atcatgatga
    27781 ctgcatcaaa ttcagtacta tgctggtaac ctgcaatcat agctgcttct tttccaaaat
    27841 ttctactaaa agaaataaag ctgacatgcc tatctattgt ggcaagatgt tgcatttcat
    27901 ctatagtggt atccgtacta ccatcatcta tgaaaagcat attataatca tagcctttca
    27961 cactactatc ttgtgaaagt atttcagtta attgttgatg tgtttgtgta atgacttccc
    28021 cttcattaaa acaaggaatg acgactctaa ttttcatcta tgtccatcct ttttatttaa
    28081 aagattttga atgtacctga gtattgtttt atcaaatagt aaaaaccaac tcatttattt
    28141 taatgtacac attaaaaggc attcataaac gtaaaattta acatccagtt attttaaatc
    28201 cccaattttc acatcatctg gatttttacc ttgtcttgca tttctattta aagcatcaat
    28261 ttcagctact tctgttaatt ctaaattaaa atctaaaata tctatatttt cttgaatgcg
    28321 tttaggtgtc tgagattttg gaataataat tctattgtgt gctaaatgcc aacgtaatac
    28381 aacttgtgct ggtgttttat gatatttttc agcaatttta acaatgacag ggtcgtctag
    28441 tagtcctcta tttctcatca aaggcatcca tgcagtcact ttaatatcgt gacgatcaca
    28501 aaattcttgt aattcttgtt ggttaaaata tggatgtacc tcaatttgat tcaccattgg
    28561 tttgatactt gattgagcca ttaatttttc tagatgatga acattaaaat tacatacacc
    28621 tattgctttt accttacctt gctcgtaaag ttcttccata gctttatatg tttctaaaaa
    28681 tagaccatct gcttcacaag gccaatgtat tagaaataaa tcaagataat cagtttgtaa
    28741 attttcaatc gatttgttga aatattcgaa tgttttctca taaccttgat agtcattcca
    28801 taacttcgtt gttataaaca aatcttctct atcgacgcca ttatccttta atgctcgtcc
    28861 tagtgaagcc tcattatcat aaaagtatgc tgtatcaaac gctctatagc ctgcgtcaat
    28921 tgcagcattt acaactttag tcatatcttc gtcagagatt ttataaacac ctaacccaac
    28981 tgaaggcatc gggtatccat tatttaatat ttgtatctca ttcaacatct ttatatctct
    29041 ccaatctatg tatcttttat atctttacat taccctaaat ttttcaacaa actcaattaa
    29101 tacgaattat cgctttcaat aaaaattatt cattaaatca ttaaagatat tgagttccaa
    29161 tactattttc acttttcatt aattttagtc aaaaaaataa ccaaccaaaa atgaattaaa
    29221 tcattcttag ttggtttata tattaatatc tattctgatt tttcatcttc atcagactgt
    29281 ccgatagtag gtctcgcttc attaaattca tagtttaata tcacccaaat aatttggtgg
    29341 ttatcgattt ctgaaacaac ccagcgatca taagttgtat ccacgtaatc atctttttgt
    29401 aaattggtat tacgagattg taaccatcca cctatcgtat caatatcctc agagtcatca
    29461 aattctatac cgaactcttc agttaaatca tccaatagta ctctgccatt tacttggaat
    29521 gtcttattat caattttaac gatatcattc acttcatcat catcaaattc atcacgaatt
    29581 tctccaacga tttcttctaa aatatcttcc atcgttaaaa tacctgccgt tccaccatat
    29641 tcatctataa taagactcat atgtacatgt tcacgttgca ttctaattaa tgcatcactg
    29701 atacgtgttg tctctgaaat cattggcaac tcatgtatat agtttgctat tttaatcgtt
    29761 tttccagaag cgtattcagt taaaaattct ttgacgttaa taaatccttt aatgtggtct
    29821 ttatcaccat catcagtaat tggataacgc gtaaattgat gttcttttat tgtttctagt
    29881 aattcgtcta cattaaaagg ttcatttagt gtaatcattt gagttctagg taccattata
    29941 tcttttgcat gtctttcatc gaatgaaaag atattttgca tatatgccaa ttcagtttgg
    30001 ttgatttctc caccattata actattgtta ataataattt tgatttcttc ttctgacatt
    30061 gcatcagttt gggcatcagg atttacacca aacattctaa taataacacg tgcagaacca
    30121 ttcatcagcc aaatcaatgg tttcataatg ttaccgaaat agaacaatgg tcttgcatat
    30181 actaaagcaa gcttttcagt atgttgaata gctatagatt taggcgctaa ttcaccaagt
    30241 actacatgca aatacgtaac gattataaat gacactgcaa acgaaatcgt cgtcgttaat
    30301 gcagttggta aattgattgc ttcaaatatt gggtgtaata gcttttcaaa cgttggttca
    30361 ccaagccaac ctaaccctaa agatgttact gttataccta actgacaagc agaaagataa
    30421 taatctagat tagcaatcat cttttttact attttagcag gtttatttcc ttcatctgct
    30481 agctgttcaa ttcttgttgc tctaattttt actaatgcaa attctgaacc aacaaataca
    30541 gtggttaatg caattaatag aataaatata atcaaactaa ttatggtcga agtttccaat
    30601 taattcccta tttctaggga ttcacctcca tgttatatgt gtcactcatg ggtaacacgc
    30661 attcaaattt atcactatga cttaaattac aactacatgt tatcgccttc ccatttagac
    30721 acccccagaa aaaaatgtta ttgcttctat tttatcataa tataaagtgc ttatgttaac
    30781 agattaaatc tattgcatac atttaattat gattatttat tagctaaatg tgtgaacctt
    30841 gccttgtttt attgtagatt ttacattcat atcatcatct agaataacaa gatctgcatc
    30901 cttatttact ttaatactac cttttctatc atcgatacct aatgcaatgg cttgatttaa
    30961 acttgttact cgccataaat gatctaatgt atcacctgta aatgatatta agttacgtaa
    31021 cccatgattc atttttaaaa tactaccagc aagcgcacca tttgcaagac gtgcttgttg
    31081 cgattgaaca gttacttttt gtccacccaa atcatattct ccttcaggca taccttttgc
    31141 acgcattgca tcggtaatta aataaaaacg ttcattacct ttcatacggt aagcaattgc
    31201 aaccgatgcc ggatgagaat gagtgccatc aacaatcatt tcggtatgta gagcatcatt
    31261 caaccatgct gctccaaaaa cacctggttc tctatgttgg aatggcgtcg ctgcattata
    31321 taaatgcgtg acatgtttag ctcctcgctc aacagcttca actgcttctt cgtatgttgc
    31381 tactgtatga ccaattgaaa aaataatgtc atctttatac gtttcaagcg cttcttttgc
    31441 accttcaact tcaggtgcaa acgtcataat ctttattaat ccgttagcag tctcttgaaa
    31501 atgtttaatt ttatcgataa atgggcgtac aacgtattgc ggatgttgag caccaacttt
    31561 attttcagat ataaatggtc cttctaaatg tatacctaca atttccgctg cattgtgaac
    31621 atcttgctcc gcttcatatt tagcaatatt tgtaagtgca ttatctattt tatcagtcga
    31681 ttgcgtcatt gtagtggcca agtatgatgt cgtcccttca gacaacaaat tttcggatag
    31741 atattttaag ccatcgtatg acccatccat tgcatcttga ccataaccac catgaatatg
    31801 tatatcaata aaacctggta atacatgatg acctttagca tcaatcactt gaattttatt
    31861 tgtcgtatca ttatcaattg ctgctttatc atccacttct ccaattgcaa caatctgtcc
    31921 atctttcaca tgaatgtaac cattatcgat tttgccatct tcagtataaa ctttgccgtt
    31981 atatataatt aattctgaca cgtataaacc cttctttatt tataaatttt cactttaaga
    32041 cactcatatt ttactatatc tgcttcgttt ggcataactt tttgcaaaat attaaaaaag
    32101 tctatacatt tgtggtgtct atttcttcat tcatttatct aaaaataaaa acactttcat
    32161 atcaattcat aaaaactgaa tta
//
LOCUS       DAKTGS010000011        31159 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.011, whole genome shotgun sequence.
ACCESSION   DAKTGS010000011 DAKTGS010000000
VERSION     DAKTGS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 31159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.011"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            39..1325
                     /gene="rseP"
                     /locus_tag="O0286_000444"
     CDS             39..1325
                     /gene="rseP"
                     /locus_tag="O0286_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCY0168843.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1345..3048
                     /locus_tag="O0286_000445"
     CDS             1345..3048
                     /locus_tag="O0286_000445"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCY0168844.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            3312..7622
                     /locus_tag="O0286_000446"
     CDS             3312..7622
                     /locus_tag="O0286_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0168845.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            7912..8379
                     /gene="rimP"
                     /locus_tag="O0286_000447"
     CDS             7912..8379
                     /gene="rimP"
                     /locus_tag="O0286_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCY0168846.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            8400..9575
                     /gene="nusA"
                     /locus_tag="O0286_000448"
     CDS             8400..9575
                     /gene="nusA"
                     /locus_tag="O0286_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCY0168847.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            9596..9880
                     /locus_tag="O0286_000449"
     CDS             9596..9880
                     /locus_tag="O0286_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCY0168848.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            9877..10194
                     /locus_tag="O0286_000450"
     CDS             9877..10194
                     /locus_tag="O0286_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCY0168849.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            10199..12316
                     /gene="infB"
                     /locus_tag="O0286_000451"
     CDS             10199..12316
                     /gene="infB"
                     /locus_tag="O0286_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCY0168850.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            12702..13052
                     /gene="rbfA"
                     /locus_tag="O0286_000452"
     CDS             12702..13052
                     /gene="rbfA"
                     /locus_tag="O0286_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCY0168851.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            13221..14138
                     /gene="truB"
                     /locus_tag="O0286_000453"
     CDS             13221..14138
                     /gene="truB"
                     /locus_tag="O0286_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCY0168852.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            14153..15124
                     /gene="ribF"
                     /locus_tag="O0286_000454"
     CDS             14153..15124
                     /gene="ribF"
                     /locus_tag="O0286_000454"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCY0168853.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            15239..15508
                     /gene="rpsO"
                     /locus_tag="O0286_000455"
     CDS             15239..15508
                     /gene="rpsO"
                     /locus_tag="O0286_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCY0168854.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            15878..17974
                     /gene="pnp"
                     /locus_tag="O0286_000456"
     CDS             15878..17974
                     /gene="pnp"
                     /locus_tag="O0286_000456"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCY0168855.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            18210..19883
                     /locus_tag="O0286_000457"
     CDS             18210..19883
                     /locus_tag="O0286_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0168856.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            20139..22508
                     /locus_tag="O0286_000458"
     CDS             20139..22508
                     /locus_tag="O0286_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0168857.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            22513..23226
                     /locus_tag="O0286_000459"
     CDS             22513..23226
                     /locus_tag="O0286_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0168858.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            23257..24522
                     /locus_tag="O0286_000460"
     CDS             23257..24522
                     /locus_tag="O0286_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0168859.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            24522..25808
                     /locus_tag="O0286_000461"
     CDS             24522..25808
                     /locus_tag="O0286_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0168860.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            25808..26512
                     /locus_tag="O0286_000462"
     CDS             25808..26512
                     /locus_tag="O0286_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0168861.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            26617..27444
                     /locus_tag="O0286_000463"
     CDS             26617..27444
                     /locus_tag="O0286_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCY0168862.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            27463..27855
                     /locus_tag="O0286_000464"
     CDS             27463..27855
                     /locus_tag="O0286_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0168863.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            27889..28467
                     /gene="pgsA"
                     /locus_tag="O0286_000465"
     CDS             27889..28467
                     /gene="pgsA"
                     /locus_tag="O0286_000465"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCY0168864.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            28694..29845
                     /locus_tag="O0286_000466"
     CDS             28694..29845
                     /locus_tag="O0286_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCY0168865.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            30010..31053
                     /gene="recA"
                     /locus_tag="O0286_000467"
     CDS             30010..31053
                     /gene="recA"
                     /locus_tag="O0286_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCY0168866.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
BASE COUNT        11377 a         4196 c         6091 g         9495 t
ORIGIN      
        1 aaaataaaag aaatttataa acaatcgagg tgtagcgagt gagctattta gttacaataa
       61 ttgcatttat tattgttttt ggtgtactag taactgttca tgaatatggc catatgtttt
      121 ttgcgaaaag agcaggcatt atgtgtccag aatttgcgat cggtatgggg ccaaaaattt
      181 ttagttttag aaaaaatgaa acactttaca ctattaggtt attgcctgtt ggtggatatg
      241 ttcgtatggc aggagatggc ttagaagagc caccagtcga gcccggtatg aacgttaaaa
      301 ttaaacttaa tgaagaaaat gaaataacac atatcatatt agatgatcat cataagtttc
      361 aacaaattga agcgatcgaa gttaaaaaat gtgattttaa ggatgactta ttcatagaag
      421 gtatcactgc ttatgataat gaaagacatc attttaaaat tgctagaaag tctttctttg
      481 ttgaaaatgg tagcttagtt caaattgctc cgagagacag acaatttgca cataaaaagc
      541 catggccgaa atttttaaca ttatttgcgg gaccgttatt taactttata ttagctttag
      601 tcctatttat tggtcttgca tattatcaag gcacgcctac gtctactgta gaacaagtcg
      661 cagataagta tccagctcaa caagcaggat tacaaaaagg tgataagatc gtccaaattg
      721 gcaaatataa aatatctgaa tttgatgatg ttgataaggc gttagataaa gttaaagata
      781 ataagacgac tgttaaattt gaacgtgatg gtaaaacaaa gtcagttgaa ttaacaccta
      841 aaaagactga aaaaaaactg actaaagtaa gttcagagac gaagtatgtt ctcggattcc
      901 aaccagcgag tgaacataca ctttttaaac caattgtatt cggatttaaa agctttttaa
      961 tcggtagtac ttatattttt acagctgtag taggtatgtt ggctagtata tttacgggcg
     1021 gattctcatt tgatatgtta aatggtccgg ttggtattta tcataacgtc gactcagttg
     1081 ttaaagcggg tatcattagc ttaattggtt acactgcgtt attaagtgta aacttaggta
     1141 ttatgaattt aattcctatt cctgcactag acggtggtcg tattttattt gttatatatg
     1201 aagcgatttt cagaaaacca gttaataaaa aagcggaaac aacgattatt gctattggtg
     1261 ccattttcat ggtcgttata atgatattag taacgtggaa tgatattcga cgatatttct
     1321 tataatttag gaggataaat aattatgaag caatccaaag tttttatacc aacgatgcgt
     1381 gacgtgccat cagaagcaga agcacaaagt catcgtttat tattgaaatc gggtttgata
     1441 aaacaaagta caagtgggat ttatagttat ttaccgctag caacacgtgt gttaaataat
     1501 attactgcaa ttgtgcgaca agaaatggaa cgtatcgatt ctgttgaaat tttaatgcca
     1561 gcgttacaac aagctgaatt atgggaagaa tcaggacgtt ggggtgcata tggcccagaa
     1621 ttaatgcgtt tacaagatag acatggaaga caatttgcat taggtccaac acatgaagaa
     1681 ttagttacat caatagtaag aaatgaattg aaatcataca aacaattacc gatgacatta
     1741 ttccaaattc aatctaaatt ccgtgatgaa aagagaccac gttttggttt acttcgtggg
     1801 cgtgaattta ttatgaaaga tgcatattca ttccatgctg acgaggcatc attagatcaa
     1861 acgtatcaag atatgtatca agcgtatagc cgtatttttg agagagttgg cattaacgca
     1921 agaccagtag ttgcagattc aggtgctata ggcggtagcc atacacatga atttatggca
     1981 ttaagtgcta tcggtgagga tacaatcgtt tacagtaaag aaagtgatta tgctgctaac
     2041 atcgaaaaag cagaagtcgt ttacgaacca aatcataagc atactactgt gcaaccttta
     2101 gaaaaaattg aaacaccaaa tgttaagact gcgcaagaat tggcagactt cttaggtaga
     2161 ccagtagatg aaatcgttaa aacgatgatt ttcaaagttg atggcgaata tattatggtt
     2221 ttagtgcgtg gccatcatga aattaatgac attaaattaa aatcttattt cggcacagat
     2281 aatattgaat tagcaacaca agacgaaatt gttaatttag ttggtgcaaa tcctggttca
     2341 ctaggtcctg taattgataa agaaatcaaa atttatgcag ataattttgt gcaagattta
     2401 aataatttag ttgtcggtgc taacgaagat ggttatcact taattaatgt aaatgtaggt
     2461 agagacttca acgttgatga atatggcgat ttccgtttta ttttagaagg cgaaaagtta
     2521 agtgatggtt caggcgttgc acattttgct gaaggtattg aagttggtca agtattcaaa
     2581 ttgggtacta agtattcaga atcaatgaat gctacattct tagataacca aggaaaagct
     2641 caatctttaa ttatgggttg ttacggaatt ggaatttcta gaacgctaag tgcgattgtt
     2701 gaacaaaatc acgatgataa tggaattgtt tggcctaaat cagttactcc gtttgattta
     2761 catttaattt ctattaatcc taagaaagat gatcaacgag aactagcaga tgcactatat
     2821 gctgaattta atactaaatt tgatgtgttg tacgatgatc gtcaggaacg tgcaggtgtt
     2881 aaatttaatg atgccgattt aattggttta ccactgcgaa ttgttgttgg taaacgtgca
     2941 tcggaaggta ttgtagaagt taaagaacgt ttaacaggtg atagcgaaga agttcacatt
     3001 gatgacttaa tgactgtcat tacaaataaa tatgataact taaaataatt aagatcgaat
     3061 gaattataag agtaggaaaa agctgaaaga aatctgatgc ttatgtcctg ctcttattat
     3121 ttttgatata atgattattc gatgaaaaat gactgaagac atagtataat taaagataaa
     3181 tttgttttaa caatataatg attagccaaa tataaagcat ttaattttct atcattacta
     3241 tgctcacata atctaaatat tgttcgaaca cgtaaaagta atttctattt aaggtggtaa
     3301 ttgtcttggc aatgacagag caacaaaaat ttaaagtgct tgctgatcaa attaaaattt
     3361 caaatcaatt agatgctgaa attttaaatt caggtgaact gacacgtata gatgtttcta
     3421 acaaaaacag aacatgggaa tttcatatta cattaccaca attcttagct catgaagatt
     3481 atttattatt tataaatgca atagagcaag agtttaaaga tatcgccaac gttacatgtc
     3541 gttttacggt aacaaatggc acgaatcaag atgaacatgc aattaaatac tttgggcact
     3601 gtattgacca aacagcttta tctccaaaag ttaaaggtca attgaaacag aaaaagctta
     3661 ttatgtctgg aaaagtatta aaagtaatgg tatcaaatga cattgaacgt aatcattttg
     3721 ataaggcatg taatggaagt cttatcaaag cgtttagaaa ttgtggtttt gatatcgata
     3781 aaatcatatt cgaaacaaat gataatgatc aagaacaaaa cttagcttct ttagaagcac
     3841 atattcaaga agaagacgaa caaagtgcac gattggcaac agagaaactt gaaaaaatga
     3901 aagctgaaaa agcgaaacaa caagataaca acgaaagtgc tgtcgataag tgtcaaattg
     3961 gtaagccgat tcaaattgaa aatattaaac caattgaatc tattattgag gaagagttta
     4021 aagttgcaat agagggtgtc atttttgata taaacttaaa agaacttaaa agtggtcgcc
     4081 atatcgtaga aattaaagtg actgactata cggactcttt agttttaaaa atgtttactc
     4141 gtaaaaacaa agatgattta gaacatttta aagcgctaag tgttggtaaa tgggttaggg
     4201 ctcaaggtcg tattgaagaa gatacattta ttagagattt agttatgatg atgtctgata
     4261 ttgaagagat taaaaaagcg acaaaaaaag ataaggctga agaaaagcgt gtagaattcc
     4321 acttgcatac tgcaatgagc caaatggatg gtatacccaa tattggtgcg tatgttaaac
     4381 aggcagcaga ctggggacat ccagccattg cggttacaga ccataatgtt gtgcaagcat
     4441 ttccagatgc tcacgcagca gcggaaaaac atggcattaa aatgatatac ggtatggaag
     4501 gtatgttagt tgatgatggt gttccgattg catacaaacc acaagatgtc gtattaaaag
     4561 atgctactta tgttgtgttc gacgttgaga caactggttt atcaaatcag tatgataaaa
     4621 tcatcgagct tgcagctgtg aaagttcata acggtgaaat catcgataag tttgaaaggt
     4681 ttagtaatcc gcatgaacga ttatcggaaa cgattatcaa tttgacgcat attactgatg
     4741 atatgttagt agatgcccct gagattgaag aagtacttac agagtttaaa gaatgggttg
     4801 gcgatgcgat attcgtagcg cataatgctt cgtttgatat gggcttcatc gatacgggat
     4861 atgaacgtct tgggtttgga ccatcaacga atggtgttat cgatacttta gaattatctc
     4921 gtacgattaa tactgaatat ggtaaacatg gtttgaattt cttggctaaa aaatatggcg
     4981 tagaattaac gcaacatcac cgtgccattt atgatacaga agcaacagct tacattttca
     5041 taaaaatggt tcaacaaatg aaagaattag gcgtattaaa tcataacgaa atcaacaaaa
     5101 aactcagtaa tgaagatgca tataaacgtg caagacctag tcatgtcaca ttaattgtac
     5161 aaaaccaaca aggtcttaaa aatctattta aaattgtaag tgcatcattg gtgaagtatt
     5221 tctaccgtac acctcgaatt ccacgttcat tgttagatga atatcgtgag ggattattgg
     5281 taggtacagc gtgtgatgaa ggtgaattat ttacggcagt tatgcagaag gaccagagtc
     5341 aagttgaaaa aattgccaaa tattatgatt ttattgaaat tcaaccaccg gcactttatc
     5401 aagatttaat tgatagagag cttattagag atactgaaac attacatgaa atttatcaac
     5461 gtttaataca tgcaggtgac acagcgggta tacctgttat tgcgacagga aatgcacact
     5521 atttgtttga acatgatggt atcgcacgta aaattttaat agcatcacaa cccggcaatc
     5581 cacttaatcg ctcaacttta ccggaagcac attttagaac tacagatgaa atgttaaacg
     5641 agtttcattt tttaggtgaa gaaaaagcgc atgaaattgt tgtgaaaaat acaaacgaat
     5701 tagcagatcg aattgaacgt gttgttccta ttaaagatga attatacaca ccgcgtatgg
     5761 aaggtgctaa cgaagaaatt agagaactaa gttatgcaaa tgcgcgtaaa ctgtatggtg
     5821 aagacctgcc tcaaatcgta attgatcgat tagaaaaaga attaaaaagt attatcggta
     5881 atggatttgc ggtaatttac ttaatttcgc aacgtttagt taaaaaatca ttagatgatg
     5941 gatacttagt tggttcccgt ggttcagtag gttctagttt tgtagcgaca atgactgaga
     6001 ttactgaagt aaacccgtta ccgccacact atatttgtcc gaactgtaaa acgagtgaat
     6061 ttttcaatga tggttcagta ggatcaggat ttgatttacc tgataagacg tgtgaaactt
     6121 gtggagcgcc acttattaaa gaaggacaag atattccgtt tgaaacattt ttaggattta
     6181 agggagataa agttcctgat atcgacttaa actttagtgg tgaatatcaa ccgaatgccc
     6241 ataactacac aaaagtatta tttggtgagg ataaagtatt ccgtgcaggt acaattggta
     6301 ctgttgctga aaagactgct tttggttatg ttaaaggtta tttgaatgat caaggtatcc
     6361 acaaaagagg tgctgaaata gatcgactcg ttaaaggatg tacaggtgtt aaacgtacaa
     6421 ctggacagca tccagggggt attattgtag tacctgatta catggatatt tatgatttta
     6481 cgccgataca atatcctgcc gatgatcaaa attcagcatg gatgacgaca cattttgatt
     6541 tccattctat tcatgataat gtattaaaac ttgatatact tggacacgat gatccaacaa
     6601 tgattcgtat gcttcaagat ttatcaggaa ttgatccaaa aacaatacct gtagatgata
     6661 aagaagttat gcagatattt agtacacctg aaagtttggg tgttactgaa gatgaaattt
     6721 tatgtaaaac aggtacattt ggggtaccag aattcggtac aggattcgtg cgtcaaatgt
     6781 tagaagatac aaagccaaca acattttctg aattagttca aatctcagga ttatctcatg
     6841 gtacagatgt gtggttaggc aatgctcaag aattaattaa aaccggtata tgtgatttat
     6901 caagtgtaat tggttgtcgt gatgatatca tggtttattt aatgtatgct ggtttagaac
     6961 catcaatggc ttttaaaata atggagtcag tacgtaaagg taaaggttta actgaagaaa
     7021 tgattgaaac gatgaaagaa aatgaagtgc cagattggta tttagattca tgtcttaaaa
     7081 ttaagtacat gttccctaaa gcccatgcag cagcatacgt tttaatggca gtacgtatcg
     7141 catatttcaa agtacatcat ccactttatt actatgcatc ttactttaca attcgtgcgt
     7201 cagactttga tttaatcacg atgattaaag ataaaacaag cattcgaaat actgtaaaag
     7261 acatgtattc tcgctatatg gatctaggta aaaaagaaaa agacgtatta acagtcttgg
     7321 aaattatgaa tgaaatggcg catcgaggtt atcgaatgca accgattagt ttagaaaaga
     7381 gtcaggcgtt cgaatttatc attgaaggcg atacacttat tccgccgttc atatcagtgc
     7441 ctgggcttgg cgaaaacgtt gcgaaacgaa ttgttgaagc tcgtgacgat ggcccatttt
     7501 tatcaaaaga agatttaaac aaaaaagctg gattatctca gaaaattatt gagtatttag
     7561 atgagttagg ctcattaccg aatttaccag ataaagctca actttcgata tttgatatgt
     7621 aaaatgaaat aatcaaggta tttatttaat gcgtatggcg tagtcaaaga aatacaaaat
     7681 tgttgctgga cacaaaatta tgcccgtatt tcttttcaat gtcttacgag tctattcaaa
     7741 tgtaatggtg aaataaagga acaaactttt acaagaatct ctgattaata gtgaagtcat
     7801 ttgtttcaag cataaactta tgctataatt aagttgctta aaaattagtg aactcaggca
     7861 gaagagtggg agattcccgc tcttttctat ttgccaaaaa gggaggcctg tatgagtaaa
     7921 attacagaac aagtagaagt gattgttaaa ccaattatgg aagacttgaa ttttgaactt
     7981 gtagacgttg aatatgtcaa agagggtaga gatcattttc ttagaatctc tattgataaa
     8041 gaaggtggcg tagatttaaa tgattgtacg ctagcttctg aaaaaataag tgaagctatg
     8101 gatgcaaatg atcctattcc tgaaatgtat tatttagacg tagcgtcacc tggtgcagaa
     8161 cgtccaatta aaaaagaaca agatttccaa aatgcaataa ctaaacctgt atttgtttct
     8221 ttatatgtac caattgaagg tgaaaaggaa tggttaggca ttttacaaga agtcaataat
     8281 gaaacaattg tagtacaagt taaaatcaaa gcaagaacga aagatataga gataccgaga
     8341 gacaaaatag caaaagcacg tcacgcagtt atgatttaac gtgatgagga ggaaaaaacg
     8401 tgtcaagtaa tgaattatta ttagctactg agtatttaga aaaagaaaag aagattccta
     8461 gagcagtatt aattgatgct attgaagcag ctttaattac tgcatacaaa aagaattatg
     8521 atagtgcaag aaatgtccgt gtggaattaa atatggatca aggtactttc aaagttatcg
     8581 ctcgtaaaga tgttgttgaa gaagtatttg acgacagaga tgaagtggat ttaagtacag
     8641 cgcttgttaa aaaccctgca tatgaaattg gtgatatata cgaagaagat gtaacaccta
     8701 aagattttgg tcgtgtaggt gctcaagcag cgaaacaagc agtaatgcaa cgtcttcgtg
     8761 atgctgaacg tgaaatttta tttgaagaat ttatagacaa agaagaagac atacttactg
     8821 gaattattga ccgtgttgac catcgttatg tatatgtgaa tttaggtcgt atcgaagctg
     8881 ttttatctga agcagaaaga agtcctaacg aaaaatatat tcctaacgaa cgtatcaaag
     8941 tatatgttaa caaagtggaa caaacgacaa aaggtcctca aatctatgtt tctcgtagcc
     9001 atccaggttt attaaaacgt ttatttgaac aagaagttcc agaaatttac gatggtactg
     9061 taattgttaa atcagtagca cgtgaagctg gcgatcgctc taaaattagt gtcttctctg
     9121 aaaacaatga tatagatgct gttggtgcat gtgttggtgc taaaggcgca cgtgttgaag
     9181 ctgttgttga agagctaggt ggtgaaaaaa tcgacatcgt tcaatggaat gaagatccaa
     9241 aagtatttgt aaaaaatgct ttaagccctt ctcaagtttt agaagttatt gttgatgaaa
     9301 caaatcaatc tacagtagtt gttgttcctg attatcaatt gtcattagcg attggtaaaa
     9361 gaggacaaaa cgcacgtcta gctgctaaat taaccggctg gaaaattgat attaaatcag
     9421 aaacagatgc gcgtgaagcg ggtatctatc cagtagttga agctgaaaaa gtaactgaag
     9481 aagatgttgc tttagaagat gctgacacaa cagaatcaac cgaagaggta aatgatgttt
     9541 cagttgaaac aaatgtagag aaagaatctg aataataggt tggagtgaag tatctatgaa
     9601 aaagaaaaaa attccgatgc gaaaatgtat tctttcaaat gaaatgcatc ccaaaaaaga
     9661 tatgattcgt gttgttgtta ataaagaagg cgaaatcttt gcggatgtta ctggaaagaa
     9721 acaaggccgt ggcgcatatg tttctaaaga tgttgctatg gttgaaaaag cacaacaaaa
     9781 agaaatttta gaaaaatatt ttaaagcatc taaagagcaa ttggatcctg tttacaaaga
     9841 aattattaga ttaatttata gagaagagat cccaaaatga gtatagatca aatattaaac
     9901 tttttaggat tagcaatgag agctggtaaa gtaaaaacag gtgaatcagt cattgttaat
     9961 gagattaaaa aaggaaattt gaagctcgtt attgttgcaa atgatgcgtc tgataataca
    10021 gctaaattaa ttacagataa atgtaagagt tacaaagttc cattcagaaa gtttggaaat
    10081 cgaaatgaat tgggaatagc acttggaaaa ggtgagcgtg ttaatgtagg gattactgac
    10141 ccaggctttg ctaaaaagtt gctatcaatg atagatgaat atcataagga gtgattatat
    10201 gagtaaacaa agaatttacg aatatgcgaa agaattaaat ctaaagagta aagagattat
    10261 agatgagtta aaaagcatga atattgaggt ttcaaatcat atgcaagctt tggaagatga
    10321 ccaaattaaa gcattagata aaaagttcaa aaaagaacaa aagaacgaca ataaacaaag
    10381 cactcaaaat aatcaccaaa aatcaaacaa tcaaaaccaa aataaagggc aacaaaaaga
    10441 taacaaaaag aatcaacaac aaaataataa aggcaacaaa ggcaataaaa agaataatag
    10501 aaataataag aaaaataaca agaataataa accacaaaat caaccagctg ctccaaaaga
    10561 aataccatca aaagtgacat atcaagaagg tattacagta ggcgaatttg cggataaatt
    10621 aaatgttgaa tcatcagaaa ttatcaaaaa attattctta cttggtattg ttgctaatat
    10681 caatcaatca ttaaatcaag aaacaatcga attaattgcc gatgattatg gcgttgaggt
    10741 tgaagaagaa gttgtgatta atgaagaaga cttatcaatc tatttcgaag acgaaaaaga
    10801 tgatccagag gcaattgaga gaccagcagt tgtaacaatt atgggacatg ttgaccatgg
    10861 taaaacgact ttattagatt caattcgtca tacaaaagtt acagcaggtg aagcaggcgg
    10921 aatcactcaa catattggtg catatcaaat tgaaaacgat ggcaaaaaaa tcactttctt
    10981 agatacaccg ggacatgctg catttacaac gatgcgtgcg cgtggtgcac aagtaacaga
    11041 tattactatt ttagtagtag cagctgacga tggtgttatg ccacaaacaa ttgaagcaat
    11101 taaccatgct aaagaagcag aagtaccaat tattgttgca gtaaataaaa ttgataaacc
    11161 aacttcaaat cctgatcgag ttatgcaaga attaactgaa tatggtttaa ttcctgaaga
    11221 ttggggcggc gaaacaattt tcgttccact ttctgcatta agtggtgatg gtatcgacga
    11281 tttattagaa atgataggat tagttgcaga agttcaagaa cttaaagcaa atcctaaaaa
    11341 ccgtgctgtt ggtacagtta tcgaagctga attagataaa tcacgtggtc cttctgcatc
    11401 attattagta caaaacggta cattaaatgt tggtgatgcg attgtagttg gtaatactta
    11461 cggccgtatc cgtgcaatgg ttaatgactt aggtcaaaga atcaaaacgg ctggtccatc
    11521 aacgcctgtt gaaattacag gtattaatga tgtgccacaa gctggggatc gctttgttgt
    11581 atttagtgat gaaaaacaag ctcgtcgtat tggtgaatca agacacgaag ctagcattat
    11641 acaacaacgt caagaaagta aaaatgtttc attagataac ctgtttgaac aaatgaaaca
    11701 aggtgaaatg aaagatttaa acgttattat taaaggtgat gttcaaggtt ctgttgaagc
    11761 tttagctgca tcattaatga aaattgatgt tgaaggcgta aatgttcgta tcattcatac
    11821 agcggttggt gcaattaatg agtcagacgt gacacttgct aatgcctcaa atggtattat
    11881 cattggtttc aatgttcgtc cagacagtgg tgcaaaacgt gctgcagaag ctgaaaatgt
    11941 tgatatgcgt ttacacagag ttatttataa tgttatcgaa gaaattgaat cagcgatgaa
    12001 aggtttactt gatccagaat ttgaagaaca agttatcgga caagctgaag ttcgtcaaac
    12061 attcaaagtt tctaaagttg gtactattgc tggatgttat gttactgaag gtaaaattac
    12121 gcgaaatgct ggtgtacgta ttattcgtga tggtattgtt caatatgaag gcgaattaga
    12181 tacacttaaa cgtttcaaag atgatgctaa ggaagttgca aaaggttatg aatgtggtat
    12241 tacaattgaa aactacaatg accttaaaga aggcgatgtt atcgaagcat ttgaaatggt
    12301 tgaaattaag cgttaattaa ataaattaca agctaaaagt atagttaaga ttgatatgct
    12361 ccctataaat attgcacttt ttaagtgtct actttatagg gagcatattt gatactagct
    12421 tttggttttt tattagaata gattacctat taaaagttac gttatatgga catgattttg
    12481 tataaaattt tgtggtggcc tagaatgatt tttaatgaca aaatataatg tcgactatta
    12541 ttggaaaatt ttctgttgaa atgcctatct tacggcaaac tttatttgat tttataggct
    12601 taatttatta aaataacgtg tgagctaaaa taattgttta agcattgtta cactaaaaaa
    12661 tgcaaataac aattgaactt aaagataaag aggtgacaag aatgagcagt atgagagcag
    12721 agcgtgttgg tgaacaaatg aagaaggaat taatggatat catcaacaat aaagtcaaag
    12781 atcctcgagt tggttttatt acaattacag atgttgtttt aacaaatgat ttatcgcagg
    12841 ctaaagtatt tttaactgta ttaggtaacg ataaagaagt agaaaataca tttaaagcac
    12901 ttgataaagc aaaaggcttc attaagtctg aattaggttc tagaatgcga ttacgtatta
    12961 tgccggaatt aatgtatgaa tatgatcaat caatcgaata tggtaataaa attgaacgaa
    13021 tgattcaaga tttacacaaa caagatagat aatttagtgt taggtatctg gaaaatgttt
    13081 gataatttct taatatcggt atattaacat taaacagtta atacatagat gtgtagaaat
    13141 agttaacatt ttccagtttt tttatgaata aatttagttg atacgctatt aaaatatatt
    13201 ttaaaaaaga aggtgactat atgtataatg ggatattacc agtatataaa gagcgcggtt
    13261 taacaagtca tgacgttgta ttcaaattgc gtaaaatatt aaaaactaaa aaaataggtc
    13321 acacgggtac gcttgatccc gaagttgcag gcgtgttacc ggtatgtata ggtaatgcaa
    13381 cgagagttag tgattatgtt atggatatgg gcaaagctta tgaagcaact gtatcgatag
    13441 gaagaagtac aacgactgaa gatcaaacgg gtgatacatt ggaaacaaaa ggtgtacact
    13501 cagcagattt taataaggac gatattgacc gattgttaga aagttttaaa ggtatcattg
    13561 aacaaattcc gccgatgtac tcatccgtca aagtaaatgg taaaaaatta tatgaatatg
    13621 cgcgtaataa tgaaacagtt gaaagaccaa agcgtaaagt taatattaaa gacattgggc
    13681 gtatatctga attagatttt aaagaaaatg agtgtcattt taaaatacgc gtcatctgtg
    13741 gtaaaggtac atatattaga acgctagcaa ctgatattgg tgtgaaatta ggctttccgg
    13801 cacatatgtc gaaattaaca cgaatcgagt ctggtggatt tgtgttgaaa gatagcctta
    13861 cattagaaca aataaaagaa cttcatgagc aggattcatt gcaaaataaa ttgtttcctt
    13921 tagaatatgg attaaagggt ttgccaagca ttaaaattaa agattcgcac ataaaaaaac
    13981 gtattttaaa tgggcagaaa tttaataaaa atgaatttga taacaaaatt aaagaccaaa
    14041 ttgtatttat tgatgatgat tcagaaaaag tattagcaat ttatatggta caccctacaa
    14101 aagaatcaga aattaaacct aaaaaagtct ttaattaaag gagatagaat ttatgaaagt
    14161 catagaagtg acacatccta tacaatctaa acagtatatt acagaggatg ttgcaatggc
    14221 attcggattt ttcgatggca tgcataaagg tcatgacaaa gtctttgata tattaaacga
    14281 aatagctgag gcacgcagtt taaaaaaagc ggtgatgaca tttgatccgc atccgtctgt
    14341 cgtgttgaat cctaaaagaa aacgaacaac gtatttaacg ccactttcag ataaaatcga
    14401 aaaaattagc caacatgata ttgattattg tatagtggtt aatttttcat ctaggtttgc
    14461 taatgtgagc gtagaagatt ttgttgaaaa ttatataatt aaaaataatg taaaagaagt
    14521 cattgctggt tttgatttta cttttggtaa atttggaaaa ggtaatatga ctgtacttca
    14581 agaatatgat gcgtttaata cgacaattgt gagtaaacaa gaaattgaaa atgaaaaaat
    14641 ttctacaact tctattcgtc aagatttaat caatggtgag ttgcaaaaag cgaatgatgc
    14701 tttaggctat atatattcta ttaaaggcac tgtagtgcaa ggtgaaaaaa ggggaagaac
    14761 tattggcttc ccaacagcta acattcaacc tagtgatgat tatttgttac ctcgtaaagg
    14821 tgtttatgct gttagtattg aaatcggcac tgaaaataaa ttatatcgag gggtagctaa
    14881 cataggtgta aagccaacat ttcatgatcc taacaaagca gaagttgtca tcgaagtgaa
    14941 tatctttgac tttgaggata atatttatgg tgaacgagtg accgtgaatt ggcatcattt
    15001 cttacgtcct gagattaaat ttgatggtat cgacccatta gttaaacaaa tgaacgatga
    15061 taaatcgcgt gctaaatatt tattagcagt tgattttggt gatgaagtag cttataatat
    15121 ctagagttgc gtatagttat ataaacaatc tataccacac ctttttctta gtaggtcgaa
    15181 tctccaacgc ctaactcgga ttaaggagta ttcaaacatt ttaaggagga aattgattat
    15241 ggcaatttca caagaacgta aaaacgaaat cattaaagaa taccgtgtac acgaaactga
    15301 tactggttca ccagaagtac aaatcgctgt acttactgca gaaatcaacg cagtaaacga
    15361 acacttacgt acacacaaaa aagaccacca ttcacgtcgt ggattattaa aaatggtagg
    15421 tcgtcgtaga catttattaa actacttacg tagtaaagat attcaacgtt accgtgaatt
    15481 aattaaatca cttggtatcc gtcgttaatc ttaatataac gtctttgagg ttggggcata
    15541 tttatgttcc aaccttaatt tatattaaaa aagcttttta caaatattaa catttattat
    15601 atgttaagct aatattgagt gaataataag gttacaatga gataaagatg atataagtac
    15661 acctagagta ataatcaaga tattaaaaat aaagtatgtt tttttaaaaa atataactta
    15721 tatttatact gataagggtg ggacgataag tctattttgt aaataataga tggatatccc
    15781 gctctctttt tttccaattc aatattttat aactaatatt aaaatacgat aataaatgat
    15841 atgatataac tattagattc aagagaggag atttataatg tctcaagaaa agaaagtttt
    15901 taaaactgaa tgggcaggaa gatctttaac gattgaaaca gggcaattag ctaaacaagc
    15961 aaatggcgct gtattggttc gttatggaga tacagtcgtg ttatcgacgg caactgcatc
    16021 aaaagaacct cgtgatggag atttcttccc attaacagtg aactatgaag aaaaaatgta
    16081 cgctgcgggt aaaattcctg gtggatttaa aaagagagaa ggacgtcctg gtgacgatgc
    16141 aacattaact gcgcgattaa ttgatagacc aattagacct ttattcccta aaggatataa
    16201 gcatgatgtt caaattatga acatggtatt aagtgcagat cctgattgtt caccacaaat
    16261 ggctgcaatg attggttcat ctatggcgct tagtgtgtcg gatattccat tccaagggcc
    16321 aatcgccggt gtaaatgtgg gttatattga cggtaaatat atcattaacc caacagtaga
    16381 agaaaaagaa gtttctcgtt tagaccttga agtagctggt cataaagatg cggtaaacat
    16441 ggtagaggca ggcgctagtg agattactga acaagaaatg ttagaggcga ttttctttgg
    16501 tcatgaagag attcaacgtt tagttgattt ccaacaacaa atcgtcgacc acattcaacc
    16561 tgttaaacaa gaatttattc cagcagagcg tgatgaagcg ctagttgaac gtgtaaaatc
    16621 tttaaccgaa gaaaaaggac ttaaagaaac agttttaaca tttgataaac aacaacgaga
    16681 tgaaaatctt gataacttaa aagaagaaat cgtcaatgaa tttatcgatg aagaagatcc
    16741 agagaatgaa ttacttatta aagaagttta tgcaatttta aatgaattag tgaaagaaga
    16801 agttcgacgt ttaattgcag atgaaaaaat tagaccagac ggccgtaaac ctgatgaaat
    16861 ccgtccatta gattctgaag ttggtatttt acctagaacg catggttcag gtctatttac
    16921 acgtggtcag actcaagcac tttcagtttt aacattaggt gctttaggcg attatcaatt
    16981 aattgatggt ttaggacctg aagaagaaaa aagattcatg catcattaca acttcccgaa
    17041 tttttcagta ggtgaaactg gtccagtacg tgcgccaggt cgtcgtgaaa ttggacatgg
    17101 tgcgttaggt gaaagagcat taaaatatat tattcctgat actgctgatt tcccatatac
    17161 aattcgtatt gtaagtgagg tacttgaatc aaatggttca tcatctcaag cgtcaatttg
    17221 tggatcaaca ttagcattaa tggatgcggg cgtaccgatt aaagcaccag ttgctggtat
    17281 tgctatgggc cttgttacac gtgaagatag ctatacgatt ttaactgata tccaaggtat
    17341 ggaagatgca ttaggtgata tggactttaa agtcgctggt actaaagaag gtattacagc
    17401 aatccaaatg gatattaaaa ttgacggttt aacgcgtgaa attatcgaag aggctctaga
    17461 acaagcgaga cgtggtcgtt tagaaataat gaatcatatg ttacaaacaa ttgatcaacc
    17521 acgtactgaa ttaagtgctt acgcgccaaa agttgtaact atgacaatta aaccagataa
    17581 gattagagat gttatcggac ctggtggtaa aaaaattaac gaaattattg atgaaacagg
    17641 tgttaaatta gatattgaac aagatggtac tatctttatt ggtgctgttg atcaagctat
    17701 gataaatcgt gctcgtgaaa tcattgagga aattacacgt gaagcggaag taggtcaaac
    17761 ttatcaagcc actgttaaac gtattgaaaa atacggtgcg tttgtaggcc tattcccagg
    17821 taaagatgcg ttgcttcaca tttcacaaat ttcaaaaaat agaattgaaa aagtggaaga
    17881 tgtattaaaa atcggtgaca caattgaagt taagattact gaaattgata aacaaggtcg
    17941 agtaaatgct tcacatagag cattagaaga ataatattta aagtcatatg acgacaatgt
    18001 atcgtcatgt gattttttta tgccactttt tacgaagtga cccgttttga atttgttgta
    18061 ttgaacattt taaaacgctt tattattttg tgtgcaactg ttaattatcc tgtatgtata
    18121 gtgattaata gtgtacatca agtgtttttt aacttataat gaatagtgag tttatatatg
    18181 gacgggtaac aaatttagga ggtaagattt tgagtttaat aaagaaaaag aataaagata
    18241 ttcgcattat accattaggc ggtgttggcg aaattgctaa aaatatgtat atcgttgaag
    18301 tagacgatga aatgtttatg ttagatgctg gacttatgtt tccagaagac gaaatgctag
    18361 gtattgatat tgttatacca gacatttcat acgtacttga aaataaagat aaattgaagg
    18421 gtatattcct tacacacgga catgagcacg cgattggtgc agtgagttat gttttagaac
    18481 aattagatgc accagtatat ggatctaaat tgacaatagc gttaattaaa gaaaatatga
    18541 aagcccgtaa tattgataaa aaagttcgct actatacagt taataatgat tcaattatga
    18601 gattcaaaaa cgtgaatatt agtttcttta atacgacaca cagtattcct gatagtttag
    18661 gtgtttgtat tcacacttca tatggtgcca ttgtgtatac aggtgaattt aagtttgacc
    18721 aaagtttaca tggacattat gcaccagata ttaaacgtat ggcagagatt ggtgaagaag
    18781 gcgtatttgt cttaatcagt gattctactg aggcagagaa acctggatat aatactccgg
    18841 aaaatgtgat tgaacatcat atgtatgatg cttttgcaaa agtgcgaggt cgcttgatag
    18901 tttcatgtta tgcttcgaac tttatacgta ttcagcaagt tttaaatatt gctagcaagc
    18961 taaatcgtaa agtgtcattt ttaggaagat cacttgaaag ttcatttaat attgctcgta
    19021 aaatggggta tttcgacatt cctaaagatt tgctaattcc tataacagaa gttgataatt
    19081 atcctaaaaa tgaagtgata attatagcta ctggtatgca aggagaacct gtagaagcct
    19141 taagtcaaat ggcgcaacat aagcataaaa ttatgaatat cgaagaaggc gattctgtat
    19201 ttttagcaat tacggcttct gctaatatgg aagttatcat tgcgaataca ttaaatgagc
    19261 ttgtacgtgc tggcgcacat attattccaa ataacaaaaa gattcatgct tcaagtcatg
    19321 gttgcatgga agaattaaaa atgatgatta atattatgaa acctgaatac tttattcctg
    19381 tacaaggtga atttaaaatg cagatagcac atgcgaagct agcagctgaa gcaggtgttg
    19441 caccagaaaa gattttcctt gtggaaaaag gagatgtcat taattacaac ggtaaagata
    19501 tgatattaaa tgaaaaggta aattcaggaa atattttaat agatggcatt ggtattgggg
    19561 atgtaggaaa tatcgtgttg agagaccgtc atcttttagc agaagatggt atctttattg
    19621 ctgttgtaac gttagatcct aaaaatagac gtatagctgc gggacctgaa attcaatctc
    19681 gtgggtttgt atatgtacgt gaaagtgaag acttattacg tgaagcagaa gagaaagtac
    19741 gtgaaatagt agaggctggt ttacaagaaa aacgcataga atggtctgaa attaaacaaa
    19801 atatgcgtga tcaaattagt aaactattat tcgaaagtac aaaacgtcgt cctatgatta
    19861 ttccagtaat ttctgaaatt taatcaaaaa gtcattaaca taaaagaggt cagaacaagt
    19921 cactgaaata taatggttgt catggacaat ttacttatat tttatgatgg tcaattgaag
    19981 gggtaacgat taatctgtta tcttaagtaa attgatacat agatgatatt gttctaacct
    20041 ctttcatcgt ctgtttggac tacatattct aaacatcaaa taggaaatta tatataataa
    20101 cgtcgtttta actaaggcaa cataaggagg tgcgtcaatt ggcacaagca aaaaagaaat
    20161 cgacagctaa gaaaaaaaca acatcaaaaa aaagaacaaa ttcgaggaaa aagaagaatg
    20221 ataatccgat acgttatgtc atagctattt tagtagttgt attaatggtg ttgggtgttt
    20281 tccaattagg aataataggt cgtctaattg acagcttctt taattattta tttgggtaca
    20341 gtagatattt aacatatatt ttagtactct tagcaactgg ttttattaca tactctaaac
    20401 gtattcctaa aactagacga acggctggtt cgattgtatt gcaaattgca ttgctatttg
    20461 tatcacagtt agtttttcat tttaatagtg gtatcaaagc tgaaagagaa cctgtacttt
    20521 cttatgtgta tcagtcatac caacacagtc atttcccaaa ttttggtggc ggtgtattag
    20581 gcttttattt attagagtta agcgtacctt taatttcatt atttggtgta tgtattatta
    20641 ctattttatt attatgctca agtgttattt tattaacaaa ccatcaacat cgtgaagttg
    20701 caaaagttgc actggaaaat ataaaagctt ggtttggttc atttaatgaa aaaatgtcgg
    20761 aaagaaacca agaaaaacaa ttgaagcgtg aagaaaaagc aagacttaaa gaagaacaaa
    20821 aggcacgtca aaatgaacag ccacaaataa aagatgtgag tgattttacg gaagtgcctc
    20881 aagaaagaga tattccaatt tatgggcata ctgaaaatga aagtaaaagc cagagtcaac
    20941 caagtcgaaa aaaacgagtg tttgatgcag agaatagttc gaataacatc gtaaatcatc
    21001 atcaagcaga tcagcaagaa caattaacag aacaaactca taacagtgtt gaaagtgaaa
    21061 acactattga agaagctggt gaagttacga atgtatcgta tgttgttcca ccgttaactt
    21121 tacttaatca acctgcaaaa caaaaagcaa catctaaagc tgaagtgcaa cgtaaaggac
    21181 aagtactaga gaatacatta aaagattttg gggtaaatgc aaaagtgaca caaattaaaa
    21241 ttggtcctgc agtaactcaa tatgaaattc aaccagctca aggggttaaa gtgagtaaaa
    21301 ttgtaaactt gcataatgat attgcattag ctttagcagc aaaagatgtt agaatcgaag
    21361 cgccaatacc tggtcgttct gcagtaggta ttgaagtgcc aaatgagaaa atttcattag
    21421 tttcactaaa agaagtttta gatgaaaaat tcccgtctaa taataaacta gaagttggat
    21481 taggaagaga tatatcaggt gatccaatta ctgttccact aaatgaaatg ccacacttat
    21541 tggtggcagg atcgacgggt agtggtaaat ctgtttgtat aaatggtatt attacaagta
    21601 ttttattaaa tgctaagccg catgaagtta aacttatgtt aatcgatccg aaaatggttg
    21661 aactaaatgt ttataacgga attccacact tattaattcc ggttgttaca aatcctcata
    21721 aagctgctca agctttagaa aaaattgtag ctgagatgga aagacgttat gatttattcc
    21781 aacattcatc aactagaaac attaaaggtt ataacgaatt aatccgtaag caaaatcaag
    21841 aattagatga gaagcaacca gaattacctt atatcgttgt tattgtagat gagcttgcag
    21901 atttaatgat ggtagctggt aaagaagttg aaaatgcgat tcaacgtatt acacaaatgg
    21961 cacgtgcagc aggtatacat ttaattgtag cgacacaaag accttctgtg gatgtaatta
    22021 caggtatcat taaaaataat attccatcta gaatagcttt tgctgtgagt tctcaaacag
    22081 attcaagaac tattattggt actggcggcg cagaaaagtt acttggtaaa ggtgacatgt
    22141 tatacgttgg aaatggtgac tcatcacaaa cacgtattca aggggcgttt ttaagtgacc
    22201 aagaggtgca agatgttgta aattatgtag tagaacaaca acaggcaaat tatgtaaaag
    22261 aaatggaacc agatgcacca gtggataaat cggaaatgaa aagtgaagat gctttatatg
    22321 atgaagcgta tttgtttgtt gttgaacaac aaaaggcaag tacatcattg ttacaacgcc
    22381 aatttagaat tggttataat agagcatcta ggttgatgga tgatttagaa cgcaatcagg
    22441 taatcggtcc acaaaaagga agcaagccta gacaagtttt aatagatctt aataatgacg
    22501 aggtgtaaaa aaatgtcaga aatgaatgcg gtatataacg ttaaacaata cattttaaat
    22561 ttgattaagc aaaataaatt ggaatatggt gaccaacttc caagtaattt atcaattgcc
    22621 agagaattaa atgtaaaaac cgacgatgtt tatgaagcaa ttcaggcatt gattactgaa
    22681 caagtcatta aagataattt tgaagagggc acaagtgtta agtcactgcc ccctttcttt
    22741 tatccattga atgaacttat aagtattggg caaatgatta aaaatgcagg atttgaatgc
    22801 ggaactgaat acttaaattt tgatgagcaa ccagcaacta tgttagatgc aaatttgttg
    22861 agcgttgaag aaggatatcc agtaaccatt atagaacgat tacgaactgc cgatggagaa
    22921 ccggtcgtct attgtttaga taaaattgct aaaaaagaat taacatgtac agagtatcaa
    22981 atgagcaatg gatcgattct aagtgcgata aaagaacaaa gtaatcataa tatttgttat
    23041 gctgatacag aaattgaagc ggtaaattat gaacctcgaa tatccgaagt actgaatgct
    23101 tcgccacacg aaggtttgat tttattaaaa attacgcact ataatgaatc agatgaacct
    23161 attttgtatt cattaaatta tatgaaaaat agcttagttc aatttaaaat cactagaaaa
    23221 atatagatgt aagaagatat agaggaggct atatatttga gtagacaatc tcaaccaaat
    23281 atacatatca aagtttcacc aacaactaaa tttaaaacaa ctactatagt ttttaaattt
    23341 atggcacctt tagaatatga cacaataaca gctagatcat tattaagcaa attattagtt
    23401 cgagcaacta agaaatggcc aaccgataag tcgtttaata atcacttagc cgatttatat
    23461 ggtgcgtatg tgaatagtac aatttcaaaa ttcaaagatc agcatgtcat tacattttca
    23521 ttagaaattg ttaatgaacg ttatttaaga aacggtgaat cattatttaa tcaaggatta
    23581 gatttattac aagaaatcat ttggaatcca ttaattgaaa ataaagcatt caatgataat
    23641 tttgttaacc aagagaaaac attattagcc aaaaaaatag aagcaatggt agataataaa
    23701 gcacaatatt cgtttttaaa attactcgac catatgtttg aaaatgaagc atataaatac
    23761 ttatctacag gacaactaga acaaatccca catattactg ctgaaacact atatcataca
    23821 tatcaatcaa tgattaataa tgatcaatgt tctgtttatg ttgtcggcaa tgtagaacct
    23881 gaaagtgttg agaaacaaat acgtgaaaaa tttgcactta aaccattcga taaacatcaa
    23941 ttccaacatt ctactcatca tttacacgat gaagaagttg attatattgt tgaatatgat
    24001 gacgtggatc aagctaaatt aaatatggga taccgttttc caacacaata tggacaaagt
    24061 ggatatgctg cctttgttgt atttaacatg atgtttggag gagatccttc atctgtttta
    24121 tttaatgaag tgcgagaaaa gcaaagttta gcgtactcta tacattcaca aattgatggc
    24181 aaaaatggct atttatttgt tttgagtggg gtttcaagtg ataagtacga aactgcaaaa
    24241 gacactatta taagtgaatt tgaaaaaata aaagcaggag atttcactga agaaaaatta
    24301 gagttagcta aaaaagtaat catttctcat cgatatgaat ctgaagatcg tccgaaaagt
    24361 attatagaga ttatgcataa ccaaatatta ttagagcaac cacaaagcaa agaaacattt
    24421 ataaatgata tacagaaggt aagtcgcgaa gatattgttt ctgttgctga aaaagcattt
    24481 ttagatacaa tctatgtgtt gacaaaagga ggggataaat aatgaaagag cgttattatg
    24541 aattaataga cgaaagagta ttcgaacaag aattagaaaa tggtttacga ttatttatta
    24601 tccccaaacc aggttttcaa aagacatttg tcacttacac tacacaattt ggttcattag
    24661 ataatcaatt caaacccctt ggacaagacc aatttgttac tgtacctgat ggagttgctc
    24721 actttttaga acataaatta tttgaaaaag aagaagaaga cttatttact gcgtttgctg
    24781 aagataacgc acaagcaaat gcgtttacaa gctttgatcg tacaagctac ttgttcagtg
    24841 caactgataa tattgaaaac aacattaaac gtttacttac aatggttgaa acgccttatt
    24901 ttacaaaaga aactgttgat aaagaaaaag gtattattgc agaagaaata aaaatgtatc
    24961 aagagcaacc tggatataaa ttaatgtttg atacattgcg tgcaatgtat caacaacatc
    25021 caatacgtgt tgatattgcc ggtagtgtag aaagtatata cgatattaca aaagatgatt
    25081 tgtatctatg ttatgaaacg ttttatcatc catcaaatat ggttttattt gttgttggcg
    25141 atgtggatcc tgaagcaata tgtcgaatag taaaacaaca cgaggatgct cgtaataaag
    25201 ttaaccaacc caaaatcgaa cgaggacttg ttgatgaacc ggaggatgtt aaagaagcat
    25261 ttgttactga atctatgaaa attcaatcac caagactaat gcttggtttt aaaaataaac
    25321 cattacaaga agcgcctcaa aaatatgtac aacgtgattt agaaatgtca ttattctttg
    25381 agttaatttt tggggaagaa acagattttt atcagaattt attaaacgaa ggacttatcg
    25441 atgatacatt tggttatcaa tttgtactag agccgacgta tagtttttca atcgtgacaa
    25501 gtgctactga agaaccagat aaattaaaaa aattattatt agatgagttg cgtgataaaa
    25561 aaggcaattt ccaagatgca gaagcatttg aacttttgaa aaagcaattt ataggtgaat
    25621 tcatatcaag tttaaactca cctgaatata ttgctaatca atatactaaa ttgtattttg
    25681 aaggtgttag cgtgtttgac atgttagaca ttgttgaaaa tatcacgtta gatagtatta
    25741 acgaaacgtc atcgttatat ttaaatttag atcagcaagt cgatagtcgt ttggagatta
    25801 aaaagtaatg aaagcattag tattaggtgg ttctggttca attggttctg agatagtcaa
    25861 acaattatta actgatggat ttgaagttta tgtgcaatat tatcgtactg atataaatga
    25921 attaactagc aaatttaatg atgataaagt tcgttttata caagcggatt tatctcaaac
    25981 aattgatatt gacaaaacat ttggtgacat taaatcatta gactgtttaa tatatgcaag
    26041 tggtcagtct ttatatggtg ttttacaaga tatgaaagac catgatattg atgcatgtta
    26101 tcagttaaat gtcttgcaat taattcgatt atgtagatat ttcgttgatg ttttacgtca
    26161 aagtgccaat ggaagaatta ttgtaatttc atcaatttgg ggtgagacag gagctagtat
    26221 ggaaactatt tattcggcga tgaaaagtgc acaattaggt ttcgttaagg cgcttagtca
    26281 agagcttgca ctaacatcag tgacagtaaa tgctatcgca cctggatttg tagccggtaa
    26341 tatggcaagt gagtggcaag aagatgaact tcaagcaatg ataactgaat taccacaaca
    26401 gcgattggtt ttaccgagtg aggttgctca tacatgcgcc tatttatatc acccaaatgc
    26461 tagaagtgtt actggaacta tacagaaagt taatggtgct tggtatattt aaaatataga
    26521 actattttta tgagcaaatg tttttatgat tgatgttata atatgaatat tacaatgaat
    26581 cataatttgt taattaaaca aggggtgagg ttcaccatga cagttgcaga gaaaaaagaa
    26641 tggtacctag aatacgaaat tgaaattaat agaccgggtc ttttaggtga tgtatctagt
    26701 ttattaggta tgttaggtat aagtattgtt acaattaatg gtgttgatca aggtaaacga
    26761 ggccttttaa ttaaaacaga caatcttgaa aaagttgaac gttttgagca aatagctcgt
    26821 ggtataaatg aaattgaaat aacaaagctt aaaaaaccag aattaagaga ccgtcttgca
    26881 gtaagacatg gaagatatat tgagcaagat gcaaaagata agaaaacctt tcgatttgag
    26941 cgtgaagatt taggcttgtt agtagacttt ttagctgaat tgttcaaaga agaaggtcat
    27001 aagttgattg gcattagagg tatgccacgg gttggtaaaa ctgaatcaat tgttgcggga
    27061 agtgtttgtg cacataagag atggttattt attagttcta ctttaataaa acaaactgta
    27121 cgtagctctc taattaaagg ggaatatgat gccaatcatg tatacattat tgatggtgca
    27181 gttactgcca gagaatctaa tccaaaacat caagagcttg ttaacgaagt tatgacgtta
    27241 ccatcaatca aagtcgttga acatccagat ttatttgttg aaacaagtac ttgtacaatg
    27301 gaagattttg actatattat cgaattgaga gaaaatgaaa atcaagaaat acattacgaa
    27361 gaaatgaaga aacagacagt ccaaagtaag aataatttag attttggaga tccgtttggt
    27421 ggtggttttg gtttcttcga gtaagtgtaa ggaggctatg aattgaaaac ggtcggtgaa
    27481 gcgctaaaag gtagacgtga aaggttagga atgactttaa cagaattaga gcaacgtact
    27541 ggaattaaac gtgaaatgct agtgcatatt gaaaataatg aattcgatca actaccgaat
    27601 aaaaattaca gcgaaggatt tattagaaaa tatgcaagcg tagtaaatat tgaacctaac
    27661 caattaattc aagctcatca agatgaaatt ccatcgaacc aagccgaatg ggacgaagta
    27721 attacagttt tcaataataa taaagactta gattataaga gtaaatcaaa agagccaata
    27781 caattattag taatcatggg tattacagtt ttaataactt tattgttatg gatcatgtta
    27841 gttttaatat tttaacagaa ataaattagt gagaaatgag gatgtataat gaatattccg
    27901 aaccagatta cggtttttag agtagtgtta ataccagttt ttatattgtt tgcgttagtt
    27961 gattttggat ttggcaatgt gtcatttcta ggaggatatg aaataagaat tgagttatta
    28021 atcagtggtt ttatttttat attggcttcc cttagcgatt ttgttgatgg ttatttagct
    28081 agaaaatgga atttagttac aaatatgggg aaatttttgg atccattagc ggataaatta
    28141 ttagttgcaa gtgctttaat tgtacttgtg caactaggac taacaaattc tgtagtagca
    28201 atcattatta ttgccagaga atttgccgta actggtttac gtttactaca aattgaacaa
    28261 ggattcgtaa gtgcagctgg tcaattaggt aaaattaaaa cagcagttac tatggtagca
    28321 attacttggt tgttattagg tgatccattg gcaacattga ttggtttgtc attaggacaa
    28381 attttattat acattggcgt tatttttact atcttatctg gtattgaata cttttataaa
    28441 ggtagagatg tttttaaaca aaaataaata tttgtttata ctagatttca ttttcatatg
    28501 gaatctagtt tttttaatcc caattttaga aattagccac gcaattgttt ataatgatat
    28561 attgtaaaac aatatttgtt cattttttta gggaaaatct gtagtagcat ctgatacatt
    28621 gaatctaaaa ttgatgtgaa tttttaaatg aaatacatga aaaaatgaat taaacgatac
    28681 aagggggata taaatgtcaa ttgccattat tgctgtaggc tcagaactat tgctaggtca
    28741 aatcgctaat accaacggac aatttctatc taaagtattt aatgaaattg gacaaaatgt
    28801 attagaacat aaagttattg gagataataa aaaacgttta gaatcaagtg tacgtcatgc
    28861 gctagaaaaa tatgatactg ttattttaac aggtggctta ggtcctacga aagatgactt
    28921 aacgaagcat acagtggccc agattgttgg taaagattta gttattgatg agccttcttt
    28981 aaaatatatt gaaagctatt ttgaggaaca aggacaagaa atgacaccta ataataaaca
    29041 acaggcttta gtaattgaag gttcaactgt attaacaaat catcatggca tggctccagg
    29101 aatgatggtg aattttgaaa acaaacaaat tattttatta ccaggtccac cgaaagaaat
    29161 gcaaccaatg gtgaaaaatg aattgttgtc acattttata aaccataatc gaattataca
    29221 ttctgaacta ttaagatttg cgggaatagg tgaatctaaa gtagaaacaa tattaataga
    29281 tcttatcgat aaacagacta atcctacgat tgcgcctttg gcgggaagtc atgaagtata
    29341 tattagattg actgcaaatg ccgactcaaa agaacaagca caatcattga ttcaacctgt
    29401 taaacaagaa attcttgatc gtattggaga atattattat ggttcagatg acacattaat
    29461 tgagcaagct gtaataaaga aaattcatga accttttgta atatatgatg gtattactaa
    29521 tggtgcttta tatcatcgat tgaaagaagt ggatttaaac gatgttctaa agggtatgat
    29581 taatcacaat gaaaactttg ttgatattaa taaacctatt gagcagcaat taaaagatgc
    29641 agtgcaattt gttaataaat tgtttaatgt gtcatcagca attattctat tagagtatga
    29701 tggtgtagtt catataggct atgataataa ctttgaattt aaaactgagc aatttaaaat
    29761 gtctaaatct agaaatttat taaagaacag aagtcaaaat tatgcgctca taagattatt
    29821 aaattggctt agaacaacaa attaattgta ttatcgataa aaatataagc acgtttgttc
    29881 gtttttgcgt tttgatttca agattttata cgaacaaata ttcgcaaaac acttgtattt
    29941 tattttgaat ccttgtatag tattggtaag ataattaaaa gatagcaatt tcaattagga
    30001 ggtctcgctt tggataacga tcgtcaaaaa gctttagata cagtaattaa aaatatggag
    30061 aaatctttcg gtaaaggtgc cgtaatgaag ttgggtgaca atataggtcg ccgagtttca
    30121 actacatcaa ctggttcagt tacattagat aatgcgctag gtgtaggtgg ctatcctaaa
    30181 ggacgaatta ttgaaattta tggtcctgaa agttctggta agacaacagt agcgcttcac
    30241 gctattgctg aagtacaaag taatggcggg gtggcagcat ttatcgatgc tgaacatgct
    30301 ttagatccag aatatgctca agcattaggc gtagatatcg ataatttata tttatcgcaa
    30361 ccggatcatg gtgaacaagg tcttgaaatc gccgaagcat ttgttagaag tggtgcagtt
    30421 gatattgtag ttgtagactc agttgctgct ttaacaccta aagctgaaat tgaaggagaa
    30481 atgggagaca ctcacgttgg tttacaagct cgtttaatgt cacaagcgtt acgtaaactt
    30541 tcaggtgcta tttctaaatc aaatacaact gctattttca tcaaccaaat tcgtgaaaaa
    30601 gttggtgtta tgttcggtaa tccagagact acaccaggtg gacgtgcatt aaaattctat
    30661 agttcagtaa gactagaagt acgtcgtgca gaacagctta aacaaggaca agaaattgta
    30721 ggtaatagaa ctaaaattaa agtcgttaaa aataaagtgg caccaccatt tagagtagct
    30781 gaagttgata ttatgtatgg acaaggtatt tctaaagagg gtgaacttat tgatttaggt
    30841 gttgaaaacg acatcgttga taaatcagga gcatggtatt cttacaatgg cgaacgaatg
    30901 ggtcaaggta aggaaaatgt taaaatgtac ttgaaagaaa atccacaaat taaagaagaa
    30961 attgatcgta aattgagaga aaaattaggt atatctgatg gtgatgttga agaaacagaa
    31021 gatgcaccaa agtcattatt tgacgaagaa tagtacacaa atttatatct atagttaaac
    31081 ttagcaaata tccttatagg attgattgaa agtgatattc atctcataaa gctagaataa
    31141 tatctaactt tatgggata
//
LOCUS       DAKTGS010000012        29732 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.012, whole genome shotgun sequence.
ACCESSION   DAKTGS010000012 DAKTGS010000000
VERSION     DAKTGS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29732
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.012"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..896
                     /locus_tag="O0286_000468"
     CDS             <1..896
                     /locus_tag="O0286_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCY0168867.1"
                     /translation="TMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSLVIVNL
                     NSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGLFAYPL
                     IEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQSVPLV
                     TYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPATIKYL
                     CLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTLTFIGM
                     TTNLTIIISIIILYVYQAVFV"
     gene            1067..1198
                     /locus_tag="O0286_000469"
     CDS             1067..1198
                     /locus_tag="O0286_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168868.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(1315..2178)
                     /locus_tag="O0286_000470"
     CDS             complement(1315..2178)
                     /locus_tag="O0286_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCY0168869.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(2359..3246)
                     /locus_tag="O0286_000471"
     CDS             complement(2359..3246)
                     /locus_tag="O0286_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0168870.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            3312..3407
                     /locus_tag="O0286_000472"
     CDS             3312..3407
                     /locus_tag="O0286_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168871.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            3433..5568
                     /locus_tag="O0286_000473"
     CDS             3433..5568
                     /locus_tag="O0286_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCY0168872.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            5681..7015
                     /locus_tag="O0286_000474"
     CDS             5681..7015
                     /locus_tag="O0286_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCY0168873.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(7207..7596)
                     /locus_tag="O0286_000475"
     CDS             complement(7207..7596)
                     /locus_tag="O0286_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168874.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            7953..8261
                     /gene="rpsJ"
                     /locus_tag="O0286_000476"
     CDS             7953..8261
                     /gene="rpsJ"
                     /locus_tag="O0286_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCY0168875.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            8289..8951
                     /gene="rplC"
                     /locus_tag="O0286_000477"
     CDS             8289..8951
                     /gene="rplC"
                     /locus_tag="O0286_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCY0168876.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            8978..9601
                     /gene="rplD"
                     /locus_tag="O0286_000478"
     CDS             8978..9601
                     /gene="rplD"
                     /locus_tag="O0286_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCY0168877.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            9601..9876
                     /gene="rplW"
                     /locus_tag="O0286_000479"
     CDS             9601..9876
                     /gene="rplW"
                     /locus_tag="O0286_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCY0168878.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            9909..10742
                     /gene="rplB"
                     /locus_tag="O0286_000480"
     CDS             9909..10742
                     /gene="rplB"
                     /locus_tag="O0286_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCY0168879.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            10809..11087
                     /gene="rpsS"
                     /locus_tag="O0286_000481"
     CDS             10809..11087
                     /gene="rpsS"
                     /locus_tag="O0286_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCY0168880.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            11116..11469
                     /gene="rplV"
                     /locus_tag="O0286_000482"
     CDS             11116..11469
                     /gene="rplV"
                     /locus_tag="O0286_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCY0168881.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            11493..12146
                     /gene="rpsC"
                     /locus_tag="O0286_000483"
     CDS             11493..12146
                     /gene="rpsC"
                     /locus_tag="O0286_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCY0168882.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            12149..12583
                     /gene="rplP"
                     /locus_tag="O0286_000484"
     CDS             12149..12583
                     /gene="rplP"
                     /locus_tag="O0286_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCY0168883.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            12573..12782
                     /gene="rpmC"
                     /locus_tag="O0286_000485"
     CDS             12573..12782
                     /gene="rpmC"
                     /locus_tag="O0286_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCY0168884.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            12806..13069
                     /gene="rpsQ"
                     /locus_tag="O0286_000486"
     CDS             12806..13069
                     /gene="rpsQ"
                     /locus_tag="O0286_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCY0168885.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            13101..13469
                     /gene="rplN"
                     /locus_tag="O0286_000487"
     CDS             13101..13469
                     /gene="rplN"
                     /locus_tag="O0286_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCY0168886.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            13505..13822
                     /gene="rplX"
                     /locus_tag="O0286_000488"
     CDS             13505..13822
                     /gene="rplX"
                     /locus_tag="O0286_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCY0168887.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            13849..14388
                     /gene="rplE"
                     /locus_tag="O0286_000489"
     CDS             13849..14388
                     /gene="rplE"
                     /locus_tag="O0286_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCY0168888.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            14411..14596
                     /locus_tag="O0286_000490"
     CDS             14411..14596
                     /locus_tag="O0286_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCY0168889.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            14628..15026
                     /gene="rpsH"
                     /locus_tag="O0286_000491"
     CDS             14628..15026
                     /gene="rpsH"
                     /locus_tag="O0286_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCY0168890.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            15051..15587
                     /gene="rplF"
                     /locus_tag="O0286_000492"
     CDS             15051..15587
                     /gene="rplF"
                     /locus_tag="O0286_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCY0168891.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            15618..15977
                     /gene="rplR"
                     /locus_tag="O0286_000493"
     CDS             15618..15977
                     /gene="rplR"
                     /locus_tag="O0286_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCY0168892.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            15998..16498
                     /gene="rpsE"
                     /locus_tag="O0286_000494"
     CDS             15998..16498
                     /gene="rpsE"
                     /locus_tag="O0286_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCY0168893.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            16515..16694
                     /gene="rpmD"
                     /locus_tag="O0286_000495"
     CDS             16515..16694
                     /gene="rpmD"
                     /locus_tag="O0286_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCY0168894.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            16711..17151
                     /gene="rplO"
                     /locus_tag="O0286_000496"
     CDS             16711..17151
                     /gene="rplO"
                     /locus_tag="O0286_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCY0168895.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            17151..18443
                     /gene="secY"
                     /locus_tag="O0286_000497"
     CDS             17151..18443
                     /gene="secY"
                     /locus_tag="O0286_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCY0168896.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            18460..19107
                     /locus_tag="O0286_000498"
     CDS             18460..19107
                     /locus_tag="O0286_000498"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCY0168897.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            19300..19518
                     /gene="infA"
                     /locus_tag="O0286_000499"
     CDS             19300..19518
                     /gene="infA"
                     /locus_tag="O0286_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCY0168898.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            19550..19663
                     /gene="rpmJ"
                     /locus_tag="O0286_000500"
     CDS             19550..19663
                     /gene="rpmJ"
                     /locus_tag="O0286_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCY0168899.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            19686..20051
                     /gene="rpsM"
                     /locus_tag="O0286_000501"
     CDS             19686..20051
                     /gene="rpsM"
                     /locus_tag="O0286_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCY0168900.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            20075..20464
                     /gene="rpsK"
                     /locus_tag="O0286_000502"
     CDS             20075..20464
                     /gene="rpsK"
                     /locus_tag="O0286_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCY0168901.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            20539..21483
                     /locus_tag="O0286_000503"
     CDS             20539..21483
                     /locus_tag="O0286_000503"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCY0168902.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            21500..21868
                     /gene="rplQ"
                     /locus_tag="O0286_000504"
     CDS             21500..21868
                     /gene="rplQ"
                     /locus_tag="O0286_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCY0168903.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            22406..23215
                     /locus_tag="O0286_000505"
     CDS             22406..23215
                     /locus_tag="O0286_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0168904.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            23212..24072
                     /locus_tag="O0286_000506"
     CDS             23212..24072
                     /locus_tag="O0286_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0168905.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            24062..24868
                     /locus_tag="O0286_000507"
     CDS             24062..24868
                     /locus_tag="O0286_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0168906.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            24873..25676
                     /gene="truA"
                     /locus_tag="O0286_000508"
     CDS             24873..25676
                     /gene="truA"
                     /locus_tag="O0286_000508"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCY0168907.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            25916..26353
                     /gene="rplM"
                     /locus_tag="O0286_000509"
     CDS             25916..26353
                     /gene="rplM"
                     /locus_tag="O0286_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCY0168908.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            26367..26765
                     /gene="rpsI"
                     /locus_tag="O0286_000510"
     CDS             26367..26765
                     /gene="rpsI"
                     /locus_tag="O0286_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCY0168909.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            27310..28407
                     /locus_tag="O0286_000511"
     CDS             27310..28407
                     /locus_tag="O0286_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0168910.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNIS
                     NSFKKKINLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGREL
                     VELHLNYEKQLNWDGVEIIYNNMNPNYKVEK"
     gene            28404..28697
                     /locus_tag="O0286_000512"
     CDS             28404..28697
                     /locus_tag="O0286_000512"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168911.1"
                     /translation="MKHPKKGVLDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQ
                     YNVSIDKKSGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            28758..29501
                     /locus_tag="O0286_000513"
     CDS             28758..29501
                     /locus_tag="O0286_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168912.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            29502..>29732
                     /locus_tag="O0286_000514"
     CDS             29502..>29732
                     /locus_tag="O0286_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0168913.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITN"
BASE COUNT        10754 a         4547 c         5667 g         8764 t
ORIGIN      
        1 ttactatgat tattttatta attgcgcttg gttacttttt gaagcgcatt aattttataa
       61 aagcaacaga tagtcaagtc attgctacat tagttcttaa tgtcacactc ccatctttag
      121 ttattgtgaa tttaaacagt gcagaattaa aattgtcatt ctccatttta ccaattttaa
      181 tgattatata tggtattgtg gcgaaaatga ttgctgtctg gttttttaga aaatatgata
      241 atcacatgcg tggatcagta ggtatgatga ctggtgctat gaatattgga ttgtttgcat
      301 atccactaat tgaagtgata tggcctaaaa cgggacttat ttattttgtt atggctgata
      361 taggtggtgc ctttgtgatg tttggaatta cttactttgt aggtagctac tttagtgaag
      421 gtagtgatca attcgatttt aaatatttag gtaagaaatt attacaatca gtgccactcg
      481 ttacatacat tgtaatgttt atattgaata taagtcacat ccatttacca catgtggcaa
      541 tcgatttctt tagcatactt tcccatgcaa atatgccgtt atcaatgatt ttgctaggtg
      601 tcatgttgaa ttttactctg gaaagaaagt atatacctgc tactattaaa tatttatgtt
      661 tgcattatgg attagcatta gttgcgggat tgttagtata tgtgttgtta ccagtatcag
      721 atgatatgat taagacgaca ttgttggtca cttggatgtt cccggtaggt gttgcaatta
      781 tttcttatgg tattcagttt aaatatcgaa cactgacatt tataggtatg acgaccaatt
      841 tgacaatcat tattagtatc attattttgt acgtgtatca ggcagtgttt gtctagttta
      901 cacatttgaa aataaaatcc attttagtat tcgtagaaaa tgagagcccc ctttaccaca
      961 atatgtaaat gtatattgtg aaaagggggc ttttatttat gacatagttg ttcaactcat
     1021 acattcaagt tgataatgca gtgcgttatg cagctatcca cttcatatgt atggcgcata
     1081 cttgatatat gaatatcatc aagattttaa aagtatcgct aattttaaag tcttaaaacg
     1141 catgattaat gtcatgatgt gttattgcgt tttgttttta gagccacgta tttcctagga
     1201 catatagagg aacattcaac gtaaaattcc cgaaaattaa ttttaatcat tctataagtg
     1261 aatggttatt ttcagaagtg attacatttt tacgttacat gagtatttaa ctttctattt
     1321 caaattacct agaattatag cagctatcac gataataata atacctgccc aaatacccgt
     1381 catctgacga cgatcttttc tttctcctaa aatgaaaata ccgcctaagg ttgaaacgat
     1441 aacaagtaac tgtgataatg agaaacttgt agctacacca acttttggtt gagaatagaa
     1501 catgaacaag ttaccaattc cccaaatcac acctggcaat agattaagtg ctgttgattt
     1561 aagtgatgtt ttatgattca tggataggat aaagccacca attgccatac cgacagattg
     1621 gaagaacaat gcatcagttc caccaacacc aaagatgtca ccaagtacaa cgaaacctac
     1681 atatccaact gtagatacaa ttaaaatacc cattgctttt ttaaattcag gattatctga
     1741 ttgacgttca tttttagctt taagtgaagt aagtgctaca ccagtaacta ataatatcat
     1801 ggcgattaaa ccaaagataa tttgagtcat tgaagaccat tcacctaaaa agataacgct
     1861 gaataatgtt gtaccaacta attgcatacc tgtagaaact ggcatagtat ttgatacacc
     1921 gattaaacta atagatttaa gctgattcgc ttgtccaaac gcccataatg caccagaaat
     1981 aagaccgaca ataattacag taggattatt gaattttgcg aaaccagtaa taagtaaacc
     2041 taatccaaca atgagtgcgc ctagcgtcgt accacgaatt tggttgtaag gtccaccgcc
     2101 gacgaacaca ttaataagaa cgacacttcc ccagaataaa gcaggtaaaa gtgcgattaa
     2161 gaaatcaaga aattgcaaga ctactcactc tcctatacta tttatgcaag tgctcccctt
     2221 aattgacact tgcatgttat tgtaaagcat tctgccctaa tagtaaaaaa caactactta
     2281 taattttcaa tatttacaaa aagtagaaaa acccacaata catgctaatg atgtactgta
     2341 gggtatttgc ttggtgtttt attcagggat tgtttgatct tttacgttac gtgattcgaa
     2401 tttgaattta atgtactcat agttgatatg gaaacctaga ccattaaatc gttcgatgag
     2461 tttattattt ttattacgaa ttttaaaata aacttttcta atatcaaaat tatcaaaagc
     2521 atattgcaat gcatacgcaa gcaactcgaa agcgatacct ttcaaacggt aatctacatg
     2581 agaactgaag tatttaatct cggcgattga ctgttgtgaa tcaatttcaa ggtataaata
     2641 acctttaagt aaaccttcgc taacaaataa aaacaaatga tgatggtcgt ctaaactatc
     2701 aatgatatct tgtggtgact gtgcctgata tttaaatgta gttgtgtgta atttgctgaa
     2761 agcacgataa aaccctttgt gataaggaat gatatttggt tggtgcatat cttcttctaa
     2821 tcttgtacgg gcttctatgt aatagtcagt gaagttataa cttgcttgaa taactttcat
     2881 taatggtttg tattgttgaa tgccttcttc aaaagaaaag ttaaagacgg catcgtcagg
     2941 ttggttcgaa gtcattgctt taaatagcgt ttcaaacgta tcgacagata atacataatt
     3001 gtcagccacg aaagggccta tgactttgaa tttattttta tcgtatgcaa aagacaatat
     3061 gattgctttg attgaatcgt tttctttaat cgaaaataca ccaggtgatt caatcgcatc
     3121 ggttattaat acatctattt cattgtactg ttgaggaagt ttatataagt atgatgctga
     3181 ttcataattc gaatgttcaa tgaacgattt tatttgtgta atatcatata actgaactat
     3241 gctcatgtca ttacctccgt actttttgtt acttttatta tatagtattt caactgaaat
     3301 gaaagttaat agtgatatta acatgttaca atacatttaa cacatcattt gcaatcattg
     3361 aatttaaatc aaagattagt ggaatagatg aagcacgttc gaaataaaag aacgtatgag
     3421 aaaggataat ttatgaaatc tttaatatta gctgaaaaac catcagtcgc aagagatatt
     3481 gctgatgctt tacaaataaa tcagaagcgt aatggttact ttgaaaataa ccaatatatt
     3541 gtcacgtggg cgttaggtca tctagtgaca aatgcgacac ctgaacaata cgataaaaat
     3601 ttaaaggaat ggcgattaga agaccttcca attataccta aatatatgaa aactgttgtt
     3661 attggtaaaa caagcaaaca atttaaaaca gtaaaagcgt taattttaga taataaagtg
     3721 aaagatatta ttattgcaac agatgctgga cgagaaggtg aactagttgc aagattgatt
     3781 ttggataaag ttggtaacaa aaagccaatc cgtcgattat ggattagctc agttactaaa
     3841 aaagctattc aacaaggttt taaaaattta aaagacggtc gtcaatataa cgatttgtat
     3901 tatgcagcgt tagcgagaag tgaggcagat tggattgttg ggattaatgc aacgcgtgca
     3961 ctaacaacaa agtatgatgc acagctatcc ctgggacgtg ttcagacacc aacgattcaa
     4021 ttagtaaata cacgacaaca agagattaat cagttcaaac cacaacaata ctttacatta
     4081 tcattaacgg taaaagggtt tgattttcag ctagaatcaa atcagcgata taccaataaa
     4141 gaaactttag aacagatggt taataatttg aaaaatgtcg atggtaagat taaatctgtt
     4201 gctactaaac ataagaagtc gtatccgcaa tcactgtaca atttaacaga tttacaacaa
     4261 gatatgtata gacgttataa aattggacct aaagaaacat tgaatacact tcaaagctta
     4321 tatgagagac ataaagtcgt aacctatcca agaacagatt caaactattt aacaactgat
     4381 atggtagata ctatgaaaga acgtattcag gcgacgatgg caacaacata taaagaccaa
     4441 gcacgcccat taatgtctaa aacattttca tcaaaaatgt cgatatttaa taatcaaaaa
     4501 gtatctgatc accatgcaat tattcctaca gaagtgagac ctgtcatgtc agacttaagt
     4561 aatagagaat taaagttata cgatatgatt gtcgagcgtt ttttagaagc tttaatgcct
     4621 ccgcacgagt atgacgcgat aactgtaact ttagaggttg cagggcacac atttgttttg
     4681 aaagagaatg taacaactgt tttaggtttt aaatctatta gacaaggtga atctattaca
     4741 gagatgcaac agcctttttc agaaggcgat gaagtgaaga tttcaaaaac aaatattaga
     4801 gaacatgaaa caacacctcc agaatatttt aatgaaggtt cgttattaaa agcgatggag
     4861 aaccctcaga actttattca attgaaggat aaaaaatatg cgcaaacttt aaaacaaaca
     4921 ggtggtatcg gcacagttgc aacaagggcc gacattatcg ataaattatt taatatgaat
     4981 gccattgaat caagagacgg taaaattaaa gtaacgtcaa aaggtaaaca aatattagaa
     5041 ttagcaccag aagaattaac gtcgccactt ttaactgcac aatgggaaga aaaattactt
     5101 ttaattgaac gtggtaaata tcaggcgaaa acatttatta atgaaatgaa agattttacg
     5161 aaagatgttg taaatgggat taaaaatagt gatcgtaaat ataaacacga taatttaaca
     5221 accacagaat gcccaacgtg tggtaaattc atgattaaag ttaaaactaa aaatggtcag
     5281 atgcttgtgt gccaagatcc atcttgtaag acgaaaaaga atgtacagcg caaaacaaat
     5341 gcaagatgtc caaactgtaa aaagaaatta acgttgtttg gtaaagggaa agaagcggta
     5401 tatcgttgtg tttgtggaca ttctgaaacg caagcacata tggatcagcg tatgaagtct
     5461 aaatcctctg gtaaagtatc tcgtaaagaa atgaaaaagt atatgaataa aaatgaaggt
     5521 ttagacaata atccgtttaa agatgcatta aagaacttga atttatagat aaaatcgaac
     5581 aaagttgaat cagaaaaacg aaaagttcgc ttttggtatt gttttttatt aagaatgata
     5641 ttaaactatt aaggtatttt aaaaaaagga gcatccattc gtgaaaaact atttccagtt
     5701 cgataaatat ggaacaaact ttaaaagaga aatcttaggc ggtatcacaa ctttcttatc
     5761 tatggcctat attttagcag ttaacccgca agttttaagt ttagcaggtg ttaaaggcgt
     5821 atcagaagat atgaaaatgg accaaggtgc catttttgta gcgactgcat tagcagcatt
     5881 tgtaggctcg ctattcatgg ggctaatagc taaatatcca attgcgttag caccaggtat
     5941 gggattaaat gcattctttg catttactgt tgtgttaacc atgggtattc cttggcaagt
     6001 tggtttaaca ggcgttttat tctcaggtat attctttgcg atattaaccg tcacagggtt
     6061 tagggaagtt attattaatg caattcctta tcaaatgaag atggctgttt cagcaggtat
     6121 aggattattc attacatttg taggtttaca aagttcgggt attatagtta aaaatgaatc
     6181 tacattagta acattgggac atttaactga tccaccagta cttttggcga tattcggtat
     6241 cgttattaca gttattttat atgcaattaa attgccgggt tctatcttta tcggtatgat
     6301 catcacagca atcgttggta tgttcactgg attgattcaa atgccatctg gtattgtggg
     6361 taagattcca agtattgaac caacgttcgg tgctgcattt gaagcgttta aagacccaag
     6421 tcaattatta acgatacagt tcttaattgt tattttaacg ttcttattta ttgatttctt
     6481 tgatacagct ggtacattag tagctgttgc tactcaagca ggtattatga aagataataa
     6541 attaccaaga gctggtagag cattgttctc agattcttta gctacgattg taggatcagt
     6601 ttttggaaca actacaacaa cttcttatat cgaatcaact tcaggtgttg ctgtaggtgc
     6661 tagaacagga tttgctagta tcgtaacagg ttgttgtttc ctattagcgc tgttctttag
     6721 tccgttaatc gctgttgtaa caagtgcagt tacgacacca gccttagtgg tggtaggtgt
     6781 attaatggct gcaaactttg ctgagataaa ctggaaaagt tttgaagttg cagtgccagc
     6841 cttcattaca atcattatga tgcctttatc atattcaatt gcaactggta ttgcgtgtgg
     6901 atttattttc tatccaatta caatgcttat ctctaaaaaa cataaagaag tacatccgat
     6961 aatgtatgta ttaatggtac tattcattct gtacttcata tttgttcatg gttaaatcca
     7021 gacacaaaaa acgactgcca atgattggta gtcgtttttt tctttgaagt aaggataaaa
     7081 taagaagcga aataacataa agcgatatta tgaatgtctt taaatatccg atataaatat
     7141 acttctttta aaattgttgg acgtgtttct tctatattat atacctaatt aaaaaggttt
     7201 taagaattat tctctatgtc catctttgta ttgtgtgtaa atgacacgag catttttatt
     7261 aggtatatag gttggcgctg ataatacaac taggacaaat agaatcatta ggaaaaataa
     7321 aatccaatga atcaaaattc ctaagaaggg gataatagct aaaaaactac ctactatacc
     7381 aaatattgga ataatcgcta taggtctaat cgcattttgt ctattcacta ataatataat
     7441 agtaagaatt agataaagta atgcattaaa gagtaaaggc tgccatccaa aacttaaaat
     7501 aaacgttgca cctaaaaaag gaatgccata gaaaaactca caaagtatta aaaagatacc
     7561 tataagtgct aaagataatt ttggtccatt tttcatattc attcaccacc gttcttatga
     7621 ctaattatat agaataattt tcaatgataa taataaaatg attcaaggta cgaatttaca
     7681 ataaagaagg aaataaaaaa tatctcgaaa atagttgaac tgactaagta tataaagtaa
     7741 aatataaaag tatgtgttag acagatagaa aaattatgtg aaaagccttg atttatcgat
     7801 gtttttcgtg taatataact aacgttggac tttaagaaaa gcgatgaagc gaaaggttac
     7861 tgacacaccc ggccgctttg ccatggcgct gtgtaagata gttttcgtgg agaagtctat
     7921 cactaaatgt agacgaataa ggagggaaaa ttatggcaaa acaaaaaatc agaatcagat
     7981 taaaagctta tgatcaccgc gtaattgatc aatcagcaga gaagattgta gaaacagcga
     8041 aacgttctgg tgcagatgtt tctggaccaa ttccgttacc aactgagaaa tcagtttaca
     8101 caatcatccg tgccgtgcat aagtataaag attcacgtga acaattcgaa caacgtacac
     8161 acaaacgttt aatcgatatt gtaaacccaa caccaaaaac agttgacgct ttaatgggct
     8221 taaacttacc atctggtgta gacatcgaaa tcaaattata atagacaatt ttaggaggtg
     8281 gactttcgat gaccaaagga atcttaggaa gaaaaattgg gatgacacaa gtattcggag
     8341 aaaacggtga attaatccct gtaacagtag tagaagctaa agaaaatgtt gtattacaaa
     8401 agaaaactgt agaagttgat ggatacaacg caatccaagt tggatttgaa gacaaaaaag
     8461 catacaaaaa agatgcaaaa tctaataaat atgctaataa accagctgaa ggtcacgcta
     8521 aaaaagctga cgcagcacct aagcgcttca ttcgtgaatt ccgcaatgta gacgtggatg
     8581 cttacgaagt aggtcaagaa gtctcagtag atacttttgt agctggcgac gttattgacg
     8641 taacaggcgt atcaaaaggt aaaggtttcc aaggtgcaat taaacgccac ggacaatctc
     8701 gtggacctat gtcacacggt tctcatttcc acagagcacc aggttctgta ggtatggctt
     8761 cagatgcttc tagagtattt aaaggccaaa aaatgccagg acgtatgggt ggaaacactg
     8821 taactgttca aaacttagaa gtagttcaag ttgacacaga aaacaaagtt atcttagtaa
     8881 aaggtaacgt acctggacct aaaaaaggtt tagtagaaat cagaacttca attaaaaaag
     8941 gtaataaata ataagaagtg aaaggaggaa atgcataatg gctaattatg atgttttaaa
     9001 attagacgga actaaatcag gttcaatcga attaagcgat gcagtattcg gtattgagcc
     9061 aaataatagc gttttattcg aagctattaa tttacaacgt gcttcattac gtcaaggtac
     9121 gcatgctgtt aagaatcgtt cagcagtaag cggtggcgga cgtaaaccat ggaagcaaaa
     9181 aggaacaggt cgtgctcgtc aaggtacaat ccgtgctcca caatggcgtg gcggtggtat
     9241 cgtattcgga ccaactccaa gaagttatgc atacaaaatg cctaagaaaa tgcgtcgttt
     9301 agctttacgc tcagcattat ctttcaaagc tcaagagaat ggcttaactg tagttgacgc
     9361 attcaacttc gaagctccaa aaactaaaga attcaaaaat gtattatcta cattagaaca
     9421 acctaaaaaa gtattagtag ttactgaaaa cgaagatgta aatgttgaat tatcagcacg
     9481 caacatccct ggcgttcaag tgacaactgc tcaaggttta aatgttttag atatcactaa
     9541 tgctgacagc ttagtaatta ctgaagctgc tgctaaaaaa gttgaggagg tgctcggata
     9601 atggaagcaa gagatattct taagcgcccc gtaatcactg agaaatcttc tgaagcaatg
     9661 gctgaagaca aatacacttt cgacgttgat actcgtgtta acaaaacaca agtaaaaatg
     9721 gcagttgaag aaatcttcaa cgtaaaagtt gcaagtgtta atatcatgaa ttacaaacct
     9781 aagaaaaaac gtatgggccg ttaccaaggc tatacaaaca aaagaagaaa agcgattgta
     9841 actcttaaag aaggatcaat cgacttattt aactaataaa acaattacca ttaaggaggt
     9901 aagcgacaat ggctattaaa aagtataagc caataacaaa tggtcgtcgt aatatgactt
     9961 cgttagattt cgcagaaatc acgaaaacta cacctgaaaa gtcattatta aaaccgctac
    10021 cgaaaaaagc gggacgtaac aaccaaggta aattgactgt aagacaccat ggtggtggac
    10081 acaaacgtca ataccgtgtt atcgatttca aacgtaacaa agatggtatc aatgcaaaag
    10141 ttgattctat tcaatatgat ccaaaccgct cagcaaacat cgctttagtt gtatatgcag
    10201 acggtgaaaa acgatatatc attgctccta aaggattaga agtaggtcaa atcgttgaaa
    10261 gtggtgctga agctgacatc aaagttggta acgcattacc attacaaaac attccagttg
    10321 gtacagtagt acacaacatc gagcttaaac ctggtaaagg tggacaaatc gctcgttcag
    10381 ctggtgcaag tgctcaagta cttggtaaag aaggtaaata cgtattaatc agattaagat
    10441 ctggtgaagt tcgtatgatc ttatctactt gccgtgctac aatcggtcaa gttggtaacc
    10501 tacaacacga attagttaac gttggtaaag ccggacgttc aagatggaaa ggtatccgtc
    10561 caacagttcg tggttctgta atgaacccta acgatcaccc acacggtggt ggtgaaggtc
    10621 gtgctcctat cggtagacca tctccaatgt caccatgggg taaacctacg cttggtaaga
    10681 aaactcgtcg tggtaaaaaa tcatcagaca aacttatcgt tcgtggacgt aagaaaaaat
    10741 aatataatca acttatttgg gtgtgcggct taaagctgca cgcacataat aagaagggag
    10801 gcgcccaaat ggctcgtagt attaaaaaag gacctttcgt cgatgagcat ttaatgaaaa
    10861 aagttgaagc tcaagaagga agcgaaaaga aacaagtaat caaaacatgg tcacgtcgtt
    10921 ctacaatttt ccctaatttc atcggacata cttttgcagt atacgacgga cgtaaacacg
    10981 tacctgtata tgtaactgaa gatatggtag gtcataaatt aggtgagttt gctcctactc
    11041 gtacattcaa aggacacgtt gcagacgaca agaaaacaag aagataatat ctattaagta
    11101 gaggaggaca tcctaatgga agcaaaagcg gttgctagaa caataagaat cgcacctcgt
    11161 aaagtaagac tagttcttga cttaatcaga ggtaaaaatg ctgctgaagc tattgcaatt
    11221 ttaaaattaa caaacaaagc ttcatcacca gtaattgaaa aagtattaat gtccgcttta
    11281 gctaatgctg aacataacta tgacatgaac acagatgaat tagtagttaa agaagcatat
    11341 gctaacgaag gaccaacatt aaaacgtttc cgtccacgtg cgcaaggtcg tgcaagtgcg
    11401 attaacaaac gtacaagcca cattacaatc gtcgtaagtg acggtaaaga agaagctaaa
    11461 gaagcttaat taacttttaa ggagggaata ctgtgggtca aaaaattaat ccaatcggac
    11521 ttcgtgttgg tattatccgt gattgggaag ctaaatggta tgctgaaaaa gacttcgctt
    11581 cacttttaca cgaagattta aaaatccgta aatttattga taatgaatta aaagaagcat
    11641 cagtttctca cgtagagatt gaacgtgctg caaaccgtat caacattgca attcatactg
    11701 gtaaacctgg tatggtaatt ggtaaaggcg gttcagaaat cgaaaaatta cgcaacaaat
    11761 taaatgcgtt aactgataaa aaagtacaca tcaacgtaat tgaaatcaaa aaagttgatc
    11821 ttgacgctcg tttagtagct gaaaacatcg cacgtcaatt agaaaaccgt gcttcattcc
    11881 gtcgtgtaca aaaacaagca atcactagag ctatgaaact tggtgctaaa ggtatcaaaa
    11941 ctcaagtatc tggtcgttta ggcggagctg acatcgctcg tgctgaacaa tattcagaag
    12001 gaactgttcc acttcatacg ttacgtgctg acatcgatta tgcacacgct gaagctgaca
    12061 ctacttacgg taaattaggc gttaaagtat ggatttatcg tggagaagtt cttcctacta
    12121 agaacactag tggaggagga aaataataat gttactacca aaacgtgtaa aatatcgtcg
    12181 tcaacatcgt cctaaaacaa ctggtcgttc taaaggcggt aactacgtaa catttggtga
    12241 gtttggttta caagctacaa caacgtcttg gatcacatct cgtcaaatcg aatctgctcg
    12301 tatagcaatg acacgttaca tgaaacgtgg cgggaaagtt tggattaaaa tcttcccaca
    12361 tacaccatat actaaaaaac ctttagaagt acgtatgggt gctggtaaag gtgcggttga
    12421 aggctggatc gcagttgtta aaccaggtag aattttattc gaagttgctg gcgtttctga
    12481 agaagttgcg cgtgaagcac tacgtttagc aagtcacaaa cttccagtaa aaactaagtt
    12541 tgtaaaacgt gaggaattgg gtggtgaaac aaatgaaagc taaggaaatt agagacttaa
    12601 ccacttcaga aatcgaagaa caaatcaaat cttcaaaaga agagcttttt aacctacgct
    12661 ttcagttagc tacaggtcaa ttagaagaaa ctgcacgtat tcgtacagta agaaaaacga
    12721 ttgcacgtct aaaaactgtt gctcgtgaaa gagaaattga acaaagtaag gctaatcaat
    12781 aattaagtaa gaggaggtta caaaagtgag cgaaagaaac gatcgtaaag tttatgtagg
    12841 taaagttgtt tcagacaaaa tggacaagac tattacagta cttgttgaaa cttacaaaac
    12901 acacaaatta tacggtaaac gagtaaaata ctctaaaaaa tacaaaactc atgatgaaaa
    12961 caattcagct aaattaggag acattgttaa aattcaagaa actcgtcctt tatcagcaac
    13021 aaaacgtttt cgtttagtag agattgttga agagtcagta attatttaat acaagtttag
    13081 agataaggga ggtttaacta atgatccaac aagaaacacg cttgaaagta gcagacaact
    13141 ctggtgctcg tgaagttctt acaatcaaag tattaggtgg atctggtcgt aaaacagcaa
    13201 acatcggcga tgttatcgta tgtactgtta aaaatgcaac accaggtggc gttgttaaaa
    13261 aaggtgacgt tgtcaaagct gtaatcgtac gtactaagtc aggtgttcgt cgtaatgacg
    13321 gttcatacat caaatttgat gaaaatgcat gtgttatcat ccgtgatgac aaaggcccac
    13381 gtggtactcg tatcttcgga cctgttgctc gtgaattacg tgaaggtaac ttcatgaaaa
    13441 tcgtatcatt agcaccagaa gtactttaat taataacaac caatacaaat aaggaggtgc
    13501 ccacatgcat atcaaaaaag gtgacaacgt taaagttatc gcaggtaaag acaaaggtaa
    13561 agaaggtaaa gtaattgcta ctctacctaa aaaagaccgt gtcgttgtgg aaggtgttaa
    13621 cattatgaaa aaacaccaaa aaccaactca attaaatcct gaaggtggaa tcttagaaac
    13681 agaggcagca atccatgttt ctaatgtaca attattggac cctaaaacaa acgaaccaac
    13741 tcgtgtaggt tacaaatttg ttgatggtaa aaaagttcgt atcgctaaaa aatctggcga
    13801 agaaattaaa tctaataatt aataacaagt tgaaaggagg atcccacttt gaaccgttta
    13861 aaagaaaagt ttaacactga agttactgaa aacttaatga aaaaattcaa ttatagttca
    13921 gtaatggaag taccaaaaat agataaaatc gttgtgaaca tgggtgtagg tgacgcagta
    13981 caaaattcta aagtattaga caatgctgtt gaagaattag aattgatcac tggtcaaaaa
    14041 ccattagtaa ctaaagctaa aaaatcaatc gcgactttcc gtttacgtga aggtatgcca
    14101 atcggtgcga aagtaacact tcgcggtgaa agaatgtatg aattcttaga caaattaatt
    14161 tcagtatcat taccacgtgt acgtgacttc caaggtgttt ctaaaaaagc atttgacgga
    14221 cgcggtaact acactttagg tgttaaagaa caattaattt tcccagaaat cgactatgat
    14281 aaagtaagta aagttagagg aatggatatt gttatcgtaa cgactgctaa cactgatgaa
    14341 gaagctcgtg aattgttagc taacttcggt atgccattcc gtaaataatt atttaaagga
    14401 ggctaattaa gtggctaaaa cttcaatggt tgctaagcaa caaaaaaaac aaaaatatgc
    14461 agttcgtgaa tacactcgtt gtgaacgttg tggccgtcca cattctgtat atcgtaaatt
    14521 taaattatgc cgtatttgtt tccgtgaatt agcttacaaa ggccaaatcc ctggcgttcg
    14581 taaagctagc tggtaataaa aaagagtctg aaaggaggca acaatcaatg acaatgacag
    14641 atccaatcgc agatatgctt actcgtgtaa gaaacgcaaa catggtgcgt cacgagaagt
    14701 tagaattacc tgcatcaaat attaaaaaag aaattgctga aatcttaaag agtgaaggtt
    14761 tcattaaaaa tgttgaatac gtagaagatg ataaacaagg tgtacttcgt ttattcttaa
    14821 aatatggtca aaacgatgag cgtgttatca caggattaaa acgtatttca aaaccaggtt
    14881 tacgtgttta tgcaaaagct agcgaaatgc ctaaagtatt aaatggttta ggtattgcat
    14941 tagtatcaac ttctgaaggt gtaatcactg acaaagaagc aagaaaacgt aatgttggtg
    15001 gagaaattat cgcatacgtt tggtaataaa aaataaggag gtgccataac atgagtcgtg
    15061 ttggtaagaa aattattgac atccctagtg acgtaacagt aacttttgat ggaaatcatg
    15121 taactgttaa aggtcctaaa ggtgaattat caagaacttt aaatgaaaga atgacattca
    15181 aacaagaaga aaacacaatt gaagttgtaa gaccatctga ttctaaagaa gatagaacaa
    15241 accatggtac aactcgtgct ttattaaaca atatggtaca aggtgtttct caaggatacg
    15301 taaaagtact tgaacttgtt ggtgtaggtt accgtgctca aatgcaaggt aaagacttaa
    15361 tccttaacgt tggttattct cacccagtag aaattaaagc tgaagaaaac attactttct
    15421 cagttgagaa aaacacagtc gttaaagttg aaggtatttc aaaagaacaa gttggagcat
    15481 tagcatctaa catccgttca gtaagacctc cagagcctta caaaggtaaa ggtattcgtt
    15541 accaaggtga atacgttcgc cgtaaagaag gtaaaactgg taaataatag ataaactcta
    15601 aagaaaggag tattgaaatg atcagtaaaa ttgataaaaa taaagtgcgt ttaaaaagac
    15661 atgctcgtgt tcgtactaac ttatcaggta cagctgaaaa gccacgttta aacgtatatc
    15721 gttcaaacaa gcatatctac gctcaaatta ttgatgataa taaaggcgta acattagctc
    15781 aagcttcttc aaaagacagc gacattgcta ctacagcaac taaagttgaa ttagcaacta
    15841 aagtcggtga agcaattgct aaaaaagctg ctgacaaagg cattaaagaa atcgtatttg
    15901 accgtggagg atatttatat cacggacgtg ttaaagcatt agctgaagca gcaagagaaa
    15961 gcggattaga attttaattt aaaggaggga caaatacatg gctcgtagag aagaagagac
    16021 gaaagaattt gaagaacgcg ttgttacaat caaccgtgta gcaaaagttg taaaaggtgg
    16081 tcgtcgtttc cgtttcactg cattagttgt agttggagac aaaaatggtc gtgtaggttt
    16141 cggtactggt aaagctcaag aggtaccaga agcaatcaaa aaagctgttg aagcagctaa
    16201 aaaagattta gtagttgttc cacgtgttga aggtacaact ccacacacaa ttactggccg
    16261 ttacggttca ggaagcgtat ttatgaaacc ggctgcacct ggtacaggag ttatcgctgg
    16321 tggtcctgtt cgtgccgtac ttgaattagc aggtatcact gatatcttaa gtaaatcatt
    16381 aggatcaaac acaccaatca acatggttcg tgctacaatc gatggtttac aaaaccttaa
    16441 aaatgctgaa gatgttgcga aattacgtgg caaaacagta gaagaattat acaattaagg
    16501 agggaaaact agttatggct aaattacaaa ttaccctcac tcgtagtgtt attggtcgtc
    16561 ctgaaacaca acgtaaaact gttgaagctt taggtcttaa aaagactaac agttcagtag
    16621 ttgttgaaga taaccctgct attcgtgggc aaatcaacaa agttaagcac ttagtaacag
    16681 tagaagaaaa ataatcaagg aggtgccgaa atgaaattac atgagttaaa accggcagaa
    16741 ggttcacgta aagaacgcaa tcgtgttgga cgtggtgttg cgacaggtaa cggtaaaaca
    16801 agtggtcgcg gacacaaagg tcaaaaagct cgttcaggcg gtggtgtaag accaggattt
    16861 gaaggtggtc aattaccatt attccgtcgt ttaccaaaac gtggttttac taacataaat
    16921 cgtaaagaat atgctattgt taacttagac caacttaata aatttgaaga tggtactgaa
    16981 gtaactccag ctttattagt agaatctggt gttgttaaga atgaaaaatc tggtatcaaa
    17041 atactaggta atggttcact tgataagaaa ttgacagtga aagctcataa attctcagct
    17101 tcagcagcag aagctattga tgctaaaggt ggagcacacg aggtgatcta atgattcaaa
    17161 cccttgtgaa cttctttaga acaaaagagg ttcgtaacaa gattttcttc acactagcaa
    17221 tgttagtaat ttttaaaata gggacttata taccagctcc aggagtaaat cctgcagctt
    17281 ttgataatcc ccaaggttct caaggtgcca ctgagttatt aaatactttt ggtggcggag
    17341 ccttgaaacg attttctatt tttgcaatgg gtattgtacc ctacatcact gcatcaatcg
    17401 taatgcaatt attacaaatg gatattgtcc ctaaattctc agaatgggca aaacaaggtg
    17461 aagtaggtag aagaaagtta aataacgtta ctcgttattt agcaatttct ttagcattta
    17521 tccaatctat aggtatggca ttccaattta ataattatct caaaggtgcg ctgattatca
    17581 atcagtcaat tatgagttat ttattaatag cactagtttt gacagcagga actgctttct
    17641 taatatggct tggtgatcaa atcactcagt tcggtgttgg taatggtatt tctattatca
    17701 tatttgcggg tattttatca acgttgccag ccagtctaat tcaatttgga caaactgcgt
    17761 tcgttgggca agaagataca tcattagcat ggttgaaagt attaggactt ttagtgtcac
    17821 tgatactatt aactgtaggt gctatttatg tacttgaagc cgttcgtaaa attccgattc
    17881 aatatgctaa gaaacaaact gctcaaagat taggttcaca ggcaacttat ctacctttaa
    17941 aagtgaactc agccggtgtt attcctgtaa tctttgcaat ggcgttcttc ttactgccta
    18001 gaacattaac gttgttctat ccagataaag aatgggcaca aaacattgcg aatgctgcaa
    18061 atccttcaag taatgttggt atggttgtat atattgtact aattatacta ttcacatact
    18121 tctatgcatt tgtacaagtt aatccggaaa aaatggctga taaccttaag aaacaaggta
    18181 gctatgttcc aggtattaga cctggtgaac aaaccaaaaa atatattact aaagttttat
    18241 atcgtttaac ttttgtaggt tctatcttct tagccgttat ttcaattcta ccgatattag
    18301 caactaagtt tatgggatta ccacaatcaa ttcagattgg tggtacaagt ttacttatcg
    18361 ttattggtgt agcgattgaa acaatgaaat ctttagaagc tcaagtatct caaaaagaat
    18421 ataaaggctt tggtggtaga taattttagg agggcactta tgaatatcat tttgatgggt
    18481 ttacctggcg caggtaaagg aactcaagca agtgaaattg tcaagaaatt cccaataccc
    18541 cacatttcaa ctggtgacat gttcagaaaa gctataaaag aagaaactga attaggtaaa
    18601 gaagctaagt cttatatgga ccgtggcgaa ttagttcctg atgaagtgac tgtaggtatc
    18661 gttaaggaaa gaatttctga agacgatgca aaaaaaggct ttttattaga tggcttccca
    18721 agaacaatcg agcaagctga ggcattaaat aatattatgt ctgagcttga cagaaacatt
    18781 gatgctgtca tcaatatcga agttccggaa gaagaattaa tgaaccgtct tacaggtcgt
    18841 cgaatctgtg agtcatgtgg tacaacgtat catcttgtat ttaatcctcc gaaggtcgaa
    18901 ggtatttgtg atatcgatgg tggtaaattg tatcaacgag aagatgataa tcctgaaacg
    18961 gtagctaatc gtttgagtgt taatattaaa caatctaagc ctattttaga tttctatgat
    19021 caaaaaggtg tattgaaaaa tattgatggt tcaaaagata ttagcgacgt taccaaagat
    19081 gtcattgata ttttagatca tttgtaatag atcaactttt agcggtctat tttaagtgag
    19141 gcatgattta atcatattag atagatgaga gtggcaatta acgataatta attcccgtaa
    19201 tttttgttta acttataaaa ttaatcactg atagattcgt aacaataaag ttgtctattg
    19261 acgattacct tactattgca aaaaggggga agttaatcaa tggctaaaca agatgtaatt
    19321 gaattagaag gtactgtatt agatacttta ccgaacgcaa tgtttaaagt agaattagaa
    19381 aatggtcatg agattttagc tcacgtaagt ggtaaaatca gaatgaatta cattcgtatt
    19441 ctacctggcg acaaagtaac tgttgagatg tctccgtacg atttaacacg cggaagaatt
    19501 acttatcgtt ataaataatc gtcactccat aatataggga ggtataaaaa tgaaagtaag
    19561 accatcagta aaacctattt gcgaaaaatg taaagtcatt aaacgtaaag gtaaagtaat
    19621 ggtaatttgt gaaaatccaa aacacaaaca aagacaaggt taataaaaga gaggtgtaaa
    19681 ttaatatggc acgtattgca ggagtagata ttccacgtga aaaacgcgta gttatctcat
    19741 taacttatat atacggtatc ggtacgtcaa ctgctcaaaa aattcttgaa gaagctaacg
    19801 tatcagctga tactcgtgtg aaagatttaa ctgatgacga attaggtcgc atccgtgaag
    19861 ttgtagacgg ttataaagtc gaaggtgact tacgtcgtga aactaactta aatatcaaac
    19921 gtttaatgga aatttcatca taccgtggta tccgtcaccg tcgtggttta ccagttcgtg
    19981 gtcaaaaaac gaaaaacaac gcgcgtactc gtaaaggacc agttaaaacg gtagctaaca
    20041 agaaaaaata ataggtaaag gaggcaaatt ttaaatggca cgtaaacaag tatctcgtaa
    20101 acgtagagtg aaaaagaata ttgaaaatgg tgtagcacac atccgttcaa cattcaacaa
    20161 cactattgta actatcactg atgagttcgg taatgcttta tcatggtcat cagctggtgc
    20221 attaggattc aaaggatcta aaaaatcaac accatttgca gcacaaatgg cttctgaaac
    20281 tgcatctaaa tcagctatgg agcatggttt aaaaacagtt gaagtaacag ttaaaggacc
    20341 tggtccaggt cgtgaatcag ctattcgtgc attacaatct gcaggtttag aagtaactgc
    20401 gatcagagac gttactccag tacctcataa cggttgtcgt ccaccaaaac gtcgtcgtgt
    20461 ataatttatg atggtattgt tacaggtcac tgagcaaaca ttttaaatta agtcgacgta
    20521 tataaggagg atatttaaat gatagaaatc gaaaaaccta gaattgagac aattgaaatt
    20581 agtgaagatg ctaaattcgg taagttcgtt gttgaaccac tagaacgtgg ctacggtact
    20641 acactaggaa actccttacg tcgtatccta ctatcttcat taccaggtgc agccgttaag
    20701 tatattgaaa ttgagggagt tttacatgaa ttctcagcag tagacaatgt agttgaagat
    20761 gtttctacaa ttattatgaa cattaaacaa ttagcattga aaatttactc tgaagaagat
    20821 aaaactttag aaattgatgt acgtgatgaa ggcgaagtaa cagcaagcga cattacacat
    20881 gatagtgatg ttgaaatttt aaacccagag cttaaaattg caacagtatc taaaggtggt
    20941 cacttaaaaa ttcgtctagt tgctaacaag ggtagaggtt acgcattagc agaacaaaat
    21001 aatactagtg atttaccaat tggtgtaatc cctgttgatt cattgtattc acctgttgaa
    21061 cgtgtgaact atactgttga aaatacacgt gtaggtcaaa gcagtgattt tgataaatta
    21121 acattggatg tttggactaa tggttcaatc acaccacaag aatcagtttc attagcagca
    21181 aaaataatga ctgaacactt gaatatcttc gttggtctta ctgatgaagc gcaaaacgct
    21241 gaaatcatga ttgaaaaaga agaagatcaa aaagaaaaag tattagaaat gtctattgaa
    21301 gaattagact tatctgtacg ttcatataac tgcttaaaac gcgcaggaat caattctgtt
    21361 caagagttag ctgacaaatc tgaagctgac atgatgaaag tgcgtaattt aggtcgtaaa
    21421 tctttagaag aagttaaata caaattagaa gatttaggat taggattaag aaaagaagat
    21481 tgataaagga ggttaactca tgggttacag aaaattaggt cgtacttctg atcaacgtaa
    21541 agctatgtta cgtgacttag ctacatcact tattattagt gaacgtattg aaactacaga
    21601 agctcgtgca aaagaagttc gcagtgttgt tgagaaatta atcactttag gtaaaaaagg
    21661 agatttagct tctcgtcgta atgcagctaa aactttacgt aatgttgaaa tcttaaacga
    21721 agatgaaact acacaaactg cacttcaaaa attatttggt gaaatcgcag agcgttacac
    21781 agaacgtcaa ggtggttaca ctcgtatcct taaacaaggc cctcgtcgcg gtgacggtgc
    21841 tgaatcagta attatcgaat tagtataaga atttatataa atacactaac tacttttata
    21901 taaagcaaat gagtgcaacg ataatgtttg ccacatacaa atattgtcta gctcagagta
    21961 ccccatcaac taaataaaga tttaaagcgt gaactcaaca aacttatgat ggggtgcgcg
    22021 cttttttatt gaataaaata tcggacttat gaacagaaat gtttttaagt ccgatttctt
    22081 tatatttact tttttgagtg cgaatctaaa aatatgatga agctagtttg atcaatttca
    22141 ctagaactga atgaagtgaa agtattatcg ttgaatcaaa cgcgaccacc cctatattag
    22201 taaatcattt attttattaa cgtttataca taattgttag agaggaataa aagtccttat
    22261 cattagttgt taaacaacac tttaaagact agaaatgagt cagaaaatac tagtaatata
    22321 tagagaaccc agtcaatgtc atatacagcg atttataaat attgtaaaat atgatagata
    22381 cacgaatcgt aaaggagaga gacaagtgga ggataagaat tcagttattg tatttaaaaa
    22441 tgtttcattt caatatcaaa gtgatgcatc cttcacattg aaagatgttt cttttaatat
    22501 acctaaaggt cagtggacat ctattgttgg tcataacggt tctggaaaat ctacaattgc
    22561 caagttaatg attggcatag agaaagttaa atctggagaa attttttata ataatcaagc
    22621 tataactgat gataattttg aaaagttaag aaaagacata ggaattgtat ttcagaatcc
    22681 ggataatcaa tttgttggtt caattgtaaa atacgatgtg gcatttggac tcgaaaatca
    22741 tgcggttcca tatgacgaaa tgcatagaag agtcagcgaa gcacttaaac aagttgatat
    22801 gttagaacgt gcagattatg aacctaatgc attatcgggg ggacagaagc agcgtgtggc
    22861 tatagcaagt gtattagcac ttaacccctc tgtcattata ttagatgagg cgacttctat
    22921 gttagatcct gatgcacgtc aaaatttatt ggatttagtg agaaaagtta aatcagaaca
    22981 taatattaca atcatttcta ttacgcatga tttatctgag gcgatggaag cagatcatgt
    23041 tatcgttatg aataaaggga ctgtctataa agaaggcaca gcgactgaaa ttttcgacaa
    23101 tgcagaagag ttaacaagaa taggtctaga tttgccattc ccaatcaaaa taaatcaaat
    23161 gctgggacac caaacatcat tcttaactta tgaagggctg gtggatcaac tatgactata
    23221 cggtttgaca atgtaagtta tacctatcaa aaagggacac catatcagca tcaagctatt
    23281 catgatgtta atacagaatt tgaacaaggt aaatattacg ccatcgttgg acaaacgggt
    23341 agtggtaaat caacgttgat acaaaatatt aatgcgctgt taaagccgac tactgggaca
    23401 gttacagttg atgacattac tattacacat aagaccaaag ataaatatat tagacctgta
    23461 agaaaaagaa ttggaatggt atttcaattt cccgaatctc aattatttga ggacacagta
    23521 gagcgtgaaa tgatatttgg acctaaaaac tttaaaatga atttagatga agccaaaaac
    23581 tatgcccatc gtctgttgat ggatttaggc ttttcaagag atgtaatgtc acaatcacca
    23641 tttcaaatgt caggtggaca aatgcgtaaa atagcgattg tatcgatatt ggcaatgaat
    23701 cctgatatta tcgtggttga tgaacctaca gcaggacttg atccacaaag taaacgacaa
    23761 gtaatgagat tactaaagtc actacaaaca gatgaaaata aggcaattat cctaatttca
    23821 catgatatga atgaagtcgc gcgttatgca gatgaagtca tcgttatgaa agaaggtagt
    23881 atagtgtcgc aaacatcacc aaaagagctc ttcaaagata aaaaaaaatt ggcggattgg
    23941 catattggtt tgccagaaat tgttcagtta caatatgact ttgaacaaaa atatcaaaca
    24001 aaattaaaag atattgcctt aactgaagaa gcatttgtaa gcttgtataa ggagtggcaa
    24061 catgaaaaat aaattgatta tagggcgata tttaccaatt aattcttttg ttcatcatct
    24121 tgatccaaga gcaaagttaa tgtttgtttt tctttttatt atattaatat ttttctgtca
    24181 ttcaccatta acatatcttt gggtgtttgc acttatctta ttcattatga gattagcaaa
    24241 aattcaatta tggttcctaa tcaaaggttt aacaccaata tttttctttt taatcttcac
    24301 attaatgatg catatatttt taactaaagg tggatatgta ttagttgaat ggcatggcat
    24361 cacgattgaa actaatggta ttttagaagg gctatatata tcactgcgtt taattgggat
    24421 tgtaatgatt gcaacaatta tgacactatc tacaagtcca attgatttaa cagatgcgtt
    24481 tgaaagatta cttgcaccac taaagatgtt taaattacca gttcatcaat taagtatgat
    24541 aatgtccatt gcgttacgat tcatcccgac gttaatggat gagttagata aaatcatttt
    24601 ggcgcaaaag tcgcgtggtt ctgaaataag ttcagggaac attgcaacac gtattaaatc
    24661 atttattcca ttactagtgc cactattcat ctcagctttc caacgcgccg aagaattagc
    24721 ggtcgcaatg gaagttaggg gttatgatgc caatgttaag agaacgagct acagacagct
    24781 taaatggcaa ttgagagata cgatatcttt aaccatgatt atcccaattg caattatttt
    24841 attcgtatta aaatattcag gagtgtaaat tcatgcgtat attagtagaa attgcgtatc
    24901 aaggaaataa ttttctaggt tttcaaattc aacaaaatgg tcgaacggta cagcaacaat
    24961 ttgaaaagct attacaacgc atgcataaaa gacatgtgag aatccatcct tcaagtagga
    25021 ctgatagagg tgtacatgcg atacagcaat actttcattt cgacacagag ctgaatatac
    25081 ctatgtcaca atggcaatat gcaatgaacc gaacgttacc tgatgatatt tatgtcaaca
    25141 atgtagttac agtcgatgac gattttcatt gtcgttatga ttgtgtaggt aaacgttatc
    25201 gctacaaagt atatcaagca caacaccgcg atccatttca aagtggtttg aaaacattta
    25261 ttcctgaaac gttggattta ggtaaaatga acagagcggc gcaacaattc ataggtacac
    25321 atgattttac gggattttgt tcgcaaaaaa ctgaagtaga aagcaaagtt agaacattat
    25381 accaaagtga aatcgtaaag acagatgatg gctttgatta tattgtcact ggatctggat
    25441 ttttatataa catggtgcgt gtcttagttg catttttaat agaagttgga aaagggcgac
    25501 atgaagtatc tgatgtacca aaacttcttg aatcaaagaa tcgtaagaat gtacctttca
    25561 ctgcaccggc tgaaggatta tatttagaga aaatttattt agatgaaaat gagttattaa
    25621 aagactttgg taacgatatc aaaatacatc gtaaaaaatc attacaaaat gactgattgc
    25681 cattgacaaa aagcttgtga aattataaga ttattaacgg tattgtttta tatccacccc
    25741 acgataagcc ccggaaactt attgtgttac aagatatata agcagaaacg aacaacagtt
    25801 aacaaaataa atgaaattaa acgttttaaa aatgaaacaa atgaaatcat ctattaggtt
    25861 atgaaactgt ttatagcttg aatagaagca tttatttttt aggaggacaa ttattatgcg
    25921 tcaaacattt atggcaaatg aatcaaacat tgagcgcaaa tggtatgtta tcgatgctga
    25981 aggccaaaca ttaggtcgtt tatcatcaga agtagcatct atcttacgcg gtaaaaataa
    26041 agtaacttac acaccacacg ttgatactgg tgattatgta atcgttatta atgcatcaaa
    26101 aatcgaattt actggtaaca aagaaactga caaagtttac taccgtcact caaatcaccc
    26161 aggtggtatc aaatcaatca ctgctggtga attaagaaga actaacccag aacgtttaat
    26221 tgaaaactca attaaaggta tgttaccaag cactcgttta ggcgaaaaac aaggtaaaaa
    26281 attatttgta tatggtggcg ctgaacatcc acacgctgca caacaaccag aaaactacga
    26341 attacgtggt taattagaag gaggaaatga ctttggcaca agttgaatat agaggcacag
    26401 gccgtcgtaa aaactcagta gcacgtgtac gtttagtacc aggtgaaggt aacatcacag
    26461 ttaataaccg tgacgtacgc gaatacttac cattcgaatc attaatttta gacttaaacc
    26521 aaccatttga tgtaactgaa actaaaggta actatgatgt tttagttaac gttcatggtg
    26581 gtggtttcac tggacaagct caagctatcc gtcacggaat cgctcgtgca ttattagaag
    26641 cagatcctga atacagaggt tctttaaaac gcgctggatt acttactcgt gacccacgta
    26701 tgaaagaacg taaaaaacca ggtcttaaag cagctcgtcg ttcacctcaa ttctcaaaac
    26761 gttaattgtc ggacgatata tacaaaacac ctcgatatta tgtcgaggtg tttttttgcg
    26821 tttttgcggc gaatatggaa tgtgtagaat ataaatgaat ttttaccttc ccaccataaa
    26881 agatgaagaa ccatgaaatg tggagaacaa taaatagttg atattctgtt atttttttgg
    26941 aagtgaagtg atttgaatac tttactctaa acgataaaag gttaaaaaaa caacaaaaag
    27001 aaaatattac tgtcataatt ggaaatacac cgtattctgc tggtcaatct agtgtaaata
    27061 atgataataa aaaaatgaat atgaaaaatt aaatgcaaaa atagcaaata cctatgcaaa
    27121 tttttcaaca gcaaatttaa aaaaatcatt atatgattcg tatatacagg cttttcgatg
    27181 ggcaactgat agagttggaa aaaaggtatt ataggatttg taacaaatgg atcatttatt
    27241 gatagtcaaa gcactgatgg atttagaaaa gttttatacg atgaatttaa ctatctgtac
    27301 ataattaatt tgagaggcga ccaacgaaca cagggtgaaa aatcaagaaa agaaggtgga
    27361 aaaatatttg gatcaggaag tagagcaccg atagctatat ctattttagt taaagatgga
    27421 agttacaatc acgatatcta ttataatgac attggtgaat atttaactag agagcaaaaa
    27481 ttagatactt taatgaaaca tcaatcaatt gtgaatttga aaagtttgaa tgttttaccc
    27541 gataaaaaca atgattggat taatcaacga gacataaact atgagaatta tttaccaatg
    27601 tatgattcca aggatattga aaattctata tatttagatc aatttaatgg agtaaattca
    27661 gcaagagata attgggtaac aaatttttcg aatgaaaaag cattagtaaa tgcaaaatta
    27721 cttgtagata actataattc tgaaatagat agattaattg atatcttaga ttcaagagaa
    27781 cgtataaatt tagtgaacaa agatgaaaca tttattagtt ggacacgagg acttacccaa
    27841 aaattttcta aaggaaaaaa tatttcaatt aatccagaga gaattgttaa atttatgcat
    27901 agaccattta caaaaaaatg gatagtgtac gacaaaaata ttatggaaat gccaagtaga
    27961 tattataata taatggagaa tactggacag gtgatttata tacaaggaca gggaatgaat
    28021 aaagaattct cggctatgat cacggacata ttacctaatt tccaatttat tggtaatggc
    28081 aaaggctttg ctacatacaa aggtaaagat agtttgaggc ttgtagataa tatttctaat
    28141 tcttttaaaa agaaaataaa tcttaattca gaagagatag tatattatat atatgcaatt
    28201 ttgcatcata aatattatgt gaacaaatat tcaagtgatt tgagtaaagg ttttccgcga
    28261 ataccgatat taaaggatgt atatggattt gttgaaattg gtagggagtt agtggaatta
    28321 catttaaatt atgaaaaaca actcaattgg gatggtgtag aaataattta taataatatg
    28381 aatccgaatt ataaagttga aaaatgaagc accctaaaaa gggcgtgttg gatacagtaa
    28441 tatacaatac cgatattaca gttactaaca ttccaaataa agtatataat tatattgtga
    28501 atggcaagcc tgctattgaa tggattatag atcagtacaa cgtaagcatc gacaagaaat
    28561 ctggaatatt agatgatcct aatgaattta gtgaagatcc gaattacatt ttaaatctgc
    28621 ttttatcagt tattactgta agtatgaaaa ctcttggatt aatagataaa ttaccagatt
    28681 ttgaagtatt agattaagga gaggatatat tgacaaatac tatcaaaaaa ataatttatt
    28741 ttgatgaact atcagcaatg gatttattac agatagagaa gaaaggtaac cttactaaaa
    28801 cagttcagtt gatgcatgat gaaaaaggta gtgcaagtgg tgaatctaaa gtgaacatgg
    28861 aatttggtaa gcaaagcgct gtaaaacaaa tttttgattc tttacttggg atgtcagcga
    28921 gtgcttcggc tagtgcaggt ataaatggaa atataactgg agagcgagta gccaaaacaa
    28981 ttttagagaa tacattaata tatgattttt tggatacagt agagtttaga aagaaaaaaa
    29041 ctattattga tatttcagag gggtttaaac taaatattca acaagatacg atgacatatt
    29101 tcgcgactat tacccccata gcaagtatgt ttgaaggatc taaacatcta gacgaggagg
    29161 agctatcatt tgatgtttca aaaatgaatg aagcgattag aagtattaaa ggctattttg
    29221 aattaatagg aacaagaggg aatgaaaaca gagtttttag atttaatata gaatctttta
    29281 aaaataacta tagaatacaa gatttaaaat ttatgaattt aacactctac tcagtacttg
    29341 ttggtgaaat aagcaaagaa aatctaaatt acgataaaga gtttgatcta aatcaaggtg
    29401 ccaaacctag tggaattgga aagtttaagg gaattagatc agaagcaact aaagaagata
    29461 aatatcaagt gtatgatgtt attcttgcag gagtgaaata aatggaaatt catgtttata
    29521 cgggaccgct ttgtggattt aatgaatata ttaattctga agttaaagaa tatcaccgct
    29581 tcatggaatt aatcagagaa tacaatatca tagtgcgagc aagtgataca tttggaactc
    29641 atgatgttga tggtattttt gatactgaga tagaaaatct tgtgatattt tcaaacgact
    29701 ttgcttctgt aacaactcat gttattacta at
//
LOCUS       DAKTGS010000013        29714 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.013, whole genome shotgun sequence.
ACCESSION   DAKTGS010000013 DAKTGS010000000
VERSION     DAKTGS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.013"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..314)
                     /locus_tag="O0286_000515"
     CDS             complement(<1..314)
                     /locus_tag="O0286_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCY0168914.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CE"
     gene            complement(559..2310)
                     /gene="srrB"
                     /locus_tag="O0286_000516"
     CDS             complement(559..2310)
                     /gene="srrB"
                     /locus_tag="O0286_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCY0168915.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            complement(2291..3016)
                     /gene="srrA"
                     /locus_tag="O0286_000517"
     CDS             complement(2291..3016)
                     /gene="srrA"
                     /locus_tag="O0286_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCY0168916.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(3149..3886)
                     /locus_tag="O0286_000518"
     CDS             complement(3149..3886)
                     /locus_tag="O0286_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0168917.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(3879..4421)
                     /gene="scpB"
                     /locus_tag="O0286_000519"
     CDS             complement(3879..4421)
                     /gene="scpB"
                     /locus_tag="O0286_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCY0168918.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(4414..5145)
                     /locus_tag="O0286_000520"
     CDS             complement(4414..5145)
                     /locus_tag="O0286_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCY0168919.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            5237..5743
                     /locus_tag="O0286_000521"
     CDS             5237..5743
                     /locus_tag="O0286_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCY0168920.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            complement(5821..6708)
                     /gene="xerD"
                     /locus_tag="O0286_000522"
     CDS             complement(5821..6708)
                     /gene="xerD"
                     /locus_tag="O0286_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCY0168921.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(6757..7206)
                     /locus_tag="O0286_000523"
     CDS             complement(6757..7206)
                     /locus_tag="O0286_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0168922.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(7311..7853)
                     /locus_tag="O0286_000524"
     CDS             complement(7311..7853)
                     /locus_tag="O0286_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCY0168923.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            7935..8843
                     /locus_tag="O0286_000525"
     CDS             7935..8843
                     /locus_tag="O0286_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0168924.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(9059..9307)
                     /locus_tag="O0286_000526"
     CDS             complement(9059..9307)
                     /locus_tag="O0286_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168925.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(9323..10078)
                     /locus_tag="O0286_000527"
     CDS             complement(9323..10078)
                     /locus_tag="O0286_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0168926.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            10220..11035
                     /gene="proC"
                     /locus_tag="O0286_000528"
     CDS             10220..11035
                     /gene="proC"
                     /locus_tag="O0286_000528"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCY0168927.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(11140..12060)
                     /gene="rnz"
                     /locus_tag="O0286_000529"
     CDS             complement(11140..12060)
                     /gene="rnz"
                     /locus_tag="O0286_000529"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCY0168928.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            12373..13857
                     /locus_tag="O0286_000530"
     CDS             12373..13857
                     /locus_tag="O0286_000530"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCY0168929.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            14087..14953
                     /locus_tag="O0286_000531"
     CDS             14087..14953
                     /locus_tag="O0286_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0168930.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(15036..16685)
                     /locus_tag="O0286_000532"
     CDS             complement(15036..16685)
                     /locus_tag="O0286_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCY0168931.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(16701..17720)
                     /locus_tag="O0286_000533"
     CDS             complement(16701..17720)
                     /locus_tag="O0286_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0168932.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            complement(17721..17975)
                     /locus_tag="O0286_000534"
     CDS             complement(17721..17975)
                     /locus_tag="O0286_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168933.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            complement(17914..18285)
                     /locus_tag="O0286_000535"
     CDS             complement(17914..18285)
                     /locus_tag="O0286_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0168934.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            complement(18504..19910)
                     /gene="gndA"
                     /locus_tag="O0286_000536"
     CDS             complement(18504..19910)
                     /gene="gndA"
                     /locus_tag="O0286_000536"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCY0168935.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            complement(19978..21111)
                     /locus_tag="O0286_000537"
     CDS             complement(19978..21111)
                     /locus_tag="O0286_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCY0168936.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            21374..21553
                     /gene="prli42"
                     /locus_tag="O0286_000538"
     CDS             21374..21553
                     /gene="prli42"
                     /locus_tag="O0286_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCY0168937.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            complement(21680..22660)
                     /locus_tag="O0286_000539"
     CDS             complement(21680..22660)
                     /locus_tag="O0286_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0168938.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(22674..23111)
                     /locus_tag="O0286_000540"
     CDS             complement(22674..23111)
                     /locus_tag="O0286_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0168939.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            complement(23462..24736)
                     /locus_tag="O0286_000541"
     CDS             complement(23462..24736)
                     /locus_tag="O0286_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0168940.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            complement(24749..25732)
                     /locus_tag="O0286_000542"
     CDS             complement(24749..25732)
                     /locus_tag="O0286_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0168941.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            complement(25732..26724)
                     /locus_tag="O0286_000543"
     CDS             complement(25732..26724)
                     /locus_tag="O0286_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCY0168942.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            complement(26740..28161)
                     /gene="lpdA"
                     /locus_tag="O0286_000544"
     CDS             complement(26740..28161)
                     /gene="lpdA"
                     /locus_tag="O0286_000544"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0168943.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            complement(28312..>29714)
                     /gene="recN"
                     /locus_tag="O0286_000545"
     CDS             complement(28312..>29714)
                     /gene="recN"
                     /locus_tag="O0286_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCY0168944.1"
                     /translation="VKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSL
                     LKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKQELEDLESADQALLQRLDLMKF
                     QLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDENAITDRLYELSNH
                     LLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLN
                     NLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQ
                     RRIVARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKS
                     LNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYI
                     QVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIARMISGASVTDL
                     TRENAREMIQHNQRRR"
BASE COUNT         9331 a         5524 c         4225 g        10634 t
ORIGIN      
        1 tcttcacaat gcacataaac ttctacacca tcatctttac ctacaacatt tgtagcttta
       61 acttttagtc caaagttatc tttaaagaat tgttcaccta ctttttcaaa ttctttacga
      121 tgcttcttcg caaattcaat cgcatttttt tctgcaggcg gttgaaagcc ttggcctaca
      181 tattttgaag cttccatttc ttctggtaca gattttgttt ctgtttttgt gtcttttttt
      241 gattcatttt ccatcgtgga acatccccct aaaattaatg tcgtagctaa aactgatcca
      301 atgaattttt tcatatgaca cctcgaataa tttaattaat acaatatatc tatattttac
      361 cctattttta taatcaaata aataacttta tcaaacttta tatttttgta taaattagta
      421 atatatttaa tagttgatat tcgcaaaaac cctaagttct aatccacata caaaattccc
      481 cacatcaatc aaaaaacgca tgctataaat tagagtctct aatcaataac atgcgttctg
      541 aaataactat attcaatttt attctggttt tggtagttta ataataaatg ttgtgccttt
      601 ccctaattcg cttttaacat ctatggaacc accatgctct tcgataatca ttttacaaat
      661 gaacaaacct aaaccggtac cttgtttacc tcgcgttctc gctgcatcaa ctttataaaa
      721 acgatcaaat acttgttgta aatgttctgg tgcaatgcct gtacctgtat ctttaatgta
      781 taaaatatct tcgctttcat tttcatcaca agtaattgca atttcatctc caggtttcgt
      841 ataacgtgat gcattatcaa ttaagttcgt tagtacttgg tccatgcgat ccatatcata
      901 actccaaaca cgcttcttac aataattaaa agtcatattt agacctaaat catcagcttg
      961 ttggcgatac ttaattttca tcttatctag taacgctgca ataggctgaa cttctttatt
     1021 tacggataac ccttcagcat ccatgcgtgc gacatttaac aattcattaa ctaaacgatt
     1081 taaacgtttc gattcatcaa ggacaatggc aagcgattct tttatttcat ccggttctgt
     1141 aacaatacca tctacaattg attcagtata accttgaagt aatgatatcg gtgtacgtaa
     1201 ttcatgtgat acattagcaa tgaaatcttt cttcatttga tctagattgt gctcattagt
     1261 catatcacga actgtcacaa caacaccact tttacctccc tgttcaatct tgtcgatata
     1321 gcttgtggtc acaacaaaga atcgtgcatt catttctaaa tcgcgcattt cagtttgttt
     1381 tgatttaaaa gtatcttcta tttgtcttaa taagaaagct ttagcatctt catcaatatt
     1441 gtccataata tcattcgcca tcttattaga taagataatt tgtcgactct cattaatacc
     1501 taggacacct tctaccatag agttaattaa gctgtctcta atatttttag atgtggataa
     1561 tgcgtcgaca tgctcttcga tttctgtact catctgatta aatgcctgcg ataattgacc
     1621 aatttcatct ttcgttgtga cagaaggttt ataagagtaa tccccttcag atacacgtgt
     1681 agcttggtct cttaaacgtc ttaaaggttt tgtaattctt gacgataaga aaaacgcaaa
     1741 gactgttgta attgttaaga aaataacagc cgtaattatg gtgataatcg taatagcatt
     1801 atttgtatct tcgattgatt tcaagtcttt atatataaag actccactat atttgctatg
     1861 actattcttc tgtgcttttg ttggatagcc taacaaaata tatgtttgag atgagccctt
     1921 ttctttaatc gttacatttc gagtaacaga tttaccttta tcaaacacat cgtcaaaatg
     1981 gtcgttgttg actacttcat tcaacatttg ctttttaata ttagaaagtg aagccgttga
     2041 ttgacgatgt ttattattta taatcatcaa cccaccagga ttttcaatta atgtttgact
     2101 atattttatt gcttcttctt tattatgtga ttgttcgacc agtgaactta tacgtctagc
     2161 atcttctctt atggcatttt cggtttcttg tgtgaaatag tattgcataa aggtaattaa
     2221 agcaatactt aataaaatta aaactgtcgt cactattaaa ataatagtta accacagttt
     2281 aattacgaca ctatttagcc ggctcatcat tagatttaac ctcaaattta tacccaacgc
     2341 cccagactgt ttgaatcata tgcgcagctt cactagacac acgatttaac ttttctctaa
     2401 gtcgtttaac atgagtatca actgttctta aatcaccata gaattcataa tgccaaactt
     2461 cttttaataa ttgttcacgg tcaaatactt tatttggtgt tttagctaaa tatattaata
     2521 attcgtactc tttaggagtc aaattaactt cttgattatc agcaagtacg cgatgtgcat
     2581 cattatctat ttctaaatgt ttaaattcaa tcacatcacg tgcgtgaggt tcgctttgtt
     2641 ctacagttgt agattgcgtt cttcttagaa gtgctttaac tcttaagact acttctcttg
     2701 gtgaaaatgg tttgacgata taatcatctg caccagattc aaaaccttca acacggtttg
     2761 tttcttcacc tttagcagtc aacataataa tcggtgtttg tttatgttca cgcaatttag
     2821 ttgccacctg gataccatcc atttcaggca acattaaatc tagtagtatg caagcataat
     2881 tattctccat tgcaagttca taagcctctt ggccattact tgcttcatgg atttcaaaag
     2941 attctctttc taaatacatt ttaagtaatc ttctgattct atcctcatca tctacgataa
     3001 gtatttcgtt cgacatacag gtcatacctc ccacacatgc ttttctttac aaaagtatta
     3061 tatcacagga aagcgcatac agttattaaa aacctaaaat tttgtgaaaa aattctcaaa
     3121 ctttgtgata aatacatatt ttatttaatt atttcccatg ttctgctaaa tgacgcatca
     3181 cttttacttc atgaggcgtc aatacacgtc cttcaccagc attcaaaccg acaacattta
     3241 aaggcccata ttcaatacga gacagtttcg tcacttgatg accaaaatgt tcgaacattc
     3301 ttctgacttg gcgattacga ccttctgtaa ttgtaatttc aaccaatgtt gtgtttttat
     3361 ctttatcttg tttcttaact ttcacttcag ccggttgcgt cataccatct tctaattcaa
     3421 tacctttttc tagcgctttc acttcttctc tcattaaata accttttaat ttcgcaacat
     3481 attttttctt aatttgatat cttggatgtg tcattaaatt agtaaattca ccatcatttg
     3541 tgagtaataa caatccagaa gtatcatagt ctaaacgacc aacaggataa atacgtgctt
     3601 ctatatcttt aaagtaatct gtaaccactg tacgtcctct atcatcagat acacttgtta
     3661 tcacttgagt tggtttatgg aataaaatgt aaattttgtc ttctagttct attttaatac
     3721 cttcaacttc aatcgtatct gatggcttca cttttgttcc taattcagtg acagtcgtac
     3781 cattcacttt cacttttcct tcagaaatta aagtttctgc cttacgtctt gaagtataac
     3841 cgctattagc tatacgtttt tgtaatcgtt ctaattcttt agtcattatt ttctcctttt
     3901 tgattgacta gattactgaa aaaagcatcc atttcttcat cgtcttcttc agttgtcggc
     3961 aaatcttcta tattggaaat accaaataca tttaaaaata aatcagtagt aattaactgt
     4021 tggctacgct gttcattaac cactttagcc tcaactagtc ctttggcaat caatgtctta
     4081 actgcaccat ctgaattgat actacgaatt aattcaatat cacttcttga taatggctgg
     4141 ttataagcaa taattgatag tacttccatt gctgcttgtg ataatttcat ttgtgacttt
     4201 tgttcaatta attgttcaat atacgttgcc gcttcttttt tagtcgttaa aacatacgtc
     4261 attccaaatc gttgtatcat taatccatgt gatgaataat tttcaattaa ttcaacgagt
     4321 tggtctttcg acatatctaa tatttctaat agttgttttt catctaaacc ttcatcgcca
     4381 gctgtaaata aaagcgactc taatatacca tgattatcca aaatggtagt tcactcctct
     4441 aataatgtta atatcttcaa aattacgttg ctgctcaata ttaattattc ctgcttttga
     4501 catctctaaa atagctaaaa agtgagtgac tacttgttca attggctcag aaaacgtaaa
     4561 cagactaaag aagttaaaat gatctttatc tttcaatctc gatgtcactt gttctgtagc
     4621 ttgttgaatg gtaaatgtct cttttcggat ttcaacagat ttaggtgtat ttaactcaac
     4681 tctattttta actctttgat aagctacaat taattcagtt aaatcaatcg tatgatttgg
     4741 atcccaagat tcatctgttt ccaaatgaga taaatctgtc ggtctttttg taaaataaaa
     4801 atctctttct tctttcatgt catttaaaat agcagtatat tctttataat tttgatattc
     4861 tattaaacgc ccaactaaat cttcccgtgg gtcatcatca acatccatat ctgatgttga
     4921 ttgtggtaat agcatcttac ttttaatcat taagagttct gacgctaata ctaggtattc
     4981 acttgcaata ttaatttcaa gctgtttcat tgcatgaacg tactgcatat actgctctgt
     5041 taatgcttgc ataggaatat cataaatatc tatttcaaat ttttggataa gatgcagcaa
     5101 taaatctaat ggtccattga aagcatctaa tttaacttca tacataatat ctacctcgta
     5161 ttgcgtcata tgtgcataat tctctaaaat attatagcat gaaaataact tacttttaaa
     5221 ttggggtgat aacaaaatgc aacaggcact gataaatttc tattatcaat ttcatactaa
     5281 gcaacattat ttcctatgtc atgacatttt agaagatgca tggaaagctg aaaataacta
     5341 cagtaagcaa gatgcagttg ttagtttaat cttgtttgca actgcttgtt accattatcg
     5401 ccgcaataat ttaaaaggcg cctataagtc ttttaataaa tctaaagaaa taatacaaaa
     5461 tgctaaagat agagatgcgt tataccttaa tttaaatgat tatcagttgt taattgagca
     5521 acagattgca aaattgaatg cagcaaagcc tttttcatct gttatattac caataactcc
     5581 agtctttgaa cgcatcatca aggccaacta tccagactat gattataatc aggaaacagc
     5641 aacagatcct tttattgtag accatcacat gcgccgtgat cgttcagaag tgattgcagc
     5701 aaaagaagaa gcgattcaat taagaaaaca tagacgtaat taatcaacca atttaaaaaa
     5761 gccacaatag cacagacatt tttagtcatg tgtattgtga cttttgagtt attgttcact
     5821 ttatgctcta ggatgaaatt ggttatacat ttttctaatt tgagatttcg aaacatgtgt
     5881 atagagttgg gtagtagata tgtcagagtg acctaacatc tcttgcactg ctcttaaatc
     5941 tgcgccattt tccaataaat gtgtcgcaaa agagtggcgt aacgtatgtg gcgttaacgt
     6001 ctttttaatg tttgccttta caccattttg tttaatcatt ttccatattg cttgtcgtga
     6061 taaaggttta ccatgcatat ttaaaaataa gacttcagta acagtctttt ttaaaagttg
     6121 cggtctaatc gtttcaatat aagtagttaa gtactcaatg actgcgtcgc ctaatggtac
     6181 aattctttct ttatcgcctt taccaaatac gcgtacaaat cccattatta agttcacgtt
     6241 ttctaactct aaatgtatca attcagatac acgcattccc gttgcgtaca gaagttctaa
     6301 catcgtacga tcacgatatc cattaatttt atttaaatct ggcgtttcta ataaagccaa
     6361 cacttcgtca acatttaaaa cgtcaggcaa ttttttgtca tattttggtg aatctaataa
     6421 taccgttgga tctttcgccg catatttttc tcttatagca aattgatgaa aactacggat
     6481 tgttgaaata aatcgcgcaa tagatttagc agattgccct tggtctatta aatgccccaa
     6541 acactcttga attaattgtc gatctataaa atcaatatgc gagatatgat gttcagtcat
     6601 ataatcttga tactttttca aatcacgtct ataagcacca attgtgttag aacttagtcc
     6661 tttttcaatt tgtataaaac gcaaatattc ttcaataatt gtttccataa ccacacctct
     6721 aaaaaaagcg aaggttaatt taacctacgc aatttactat cctttacctt tagcttggca
     6781 cgtttcacac acaccatgga aagttaaacg atgatctaaa attttaaaat tgaactcatt
     6841 ttcaactcga ttttcaactt ctggtaacaa atcttcatcg atttcatcta cacgaccaca
     6901 ttccatacat actaaatgat ggtggaaatg ttttgcgcct tcttttctta aatcaaaacg
     6961 agcgacgcca tcaccaaagt taattttgtc gacaactttt agttcagcta acaactctaa
     7021 cgttctgtat actgtcgcca agccaatttc aggcgcttta tcttttactt tcagatatac
     7081 gtcttcagca cttagatgat ctttttcatt ttcaattaga actctaacag tagcttcgcg
     7141 ttgtggcgtt agcttatatg atgattgttg taattgttgc ttaacgcgat ttaatcgttc
     7201 ttccaacgat gtccactccc ctactaataa ttaaaatcat tacaaattat ttcaaacttc
     7261 acaattaaaa ctaacagttt tctcaataaa atgcaagctt ttctcatttg ttatttagaa
     7321 tgattataat ttaataatag gtgttgcaat gcaatgatag tcttcgcatc ttctatttct
     7381 ttattcatta gcatcgattt aacattctca atcggaactt taatgacttc gacaaattca
     7441 tcttcatcta agtgaactgt accttcttct aaattatccg taaaatatat tgataattgt
     7501 tcatcgcaaa aacctggtga accatacata tctacaacat gtgttaactc tttggcaata
     7561 tatcctgttt cttcttctaa ttcacgtttt gccgcttcga ctctatcttc atcatcttct
     7621 aatttaccag ctggaatttc tagcaatggt ttttctactg gtttacgata ctgtttcact
     7681 aatacgactt cttttttagg tgtcactgca caaacagcaa ctgcaccatt atgataaact
     7741 aattctcttg ttgacgtttc accgtttggt aatgtcactg tatgaatttc tacatctaca
     7801 attttgccat tataaataac tgttcgatca attgtttttt catttaaatc cattataatc
     7861 acgttccttt aaattcatat tatatattga tacactatgc ttgttagcta aagtgtatcg
     7921 aaaggagaac agacatgcaa aaaaatatat taaaaagtgg tatttcatta tctgaattag
     7981 gtctaggttg catgagttta ggtacagatt taaaaaaagc cgaacaaatt atagattgtg
     8041 ctgttgaaaa tggtatcact tattttgata cagcagatat gtacgataaa ggtataaatg
     8101 aatcagttgt tggtaaggca cttcttaaat atcaacaacg cgatgatatt tttattggta
     8161 caaaagtagg caatcgttta acaaaagatg gcagtacaac atgggatccg agtaaatcct
     8221 atattaaaga ggcagttaaa ggttcactaa agcgtttagg tatcgatcat atcgatttat
     8281 atcaacttca tggcggaacc attgatgacc cattagacga aacaataagc gcatttgatg
     8341 aattgaaaca agaaggaatt atacgtgctt acggtatttc ttctattcgc ccaaatgtaa
     8401 ttgattatta tttaaaacat agtcaaatcg aaacgataat gtctcaattc aatttgattg
     8461 ataatcgtcc agaatcatta ttagatgcaa ttcacaacaa tgatgttaaa gtattggcaa
     8521 gaggacctgt gtctaaagga ttattaactt caaacagtgt taatgtgctc gacaataaat
     8581 ttaaagatgg tatttttgat tattctcatg atgaattggg tgaaacaata gcctctatta
     8641 aagaaattga aagtaattta tctgcattga catttagtta tttaacatca catgacgtgc
     8701 ttggttccat cattgtaggt gcaagtagcg tcgaccaatt aaaagaaaat attgaaaact
     8761 atcatactaa agttagttta gatcagatta aaacagcaag agctcgtgta aaggatttgg
     8821 aatataccaa tcatttagtg tagaagtcat tttcagtaat aaaaacagca gcatgaggcg
     8881 tttcattata aaaatgcctt actgctgttg tttatgtaca attcgctata atttatgatt
     8941 atgattactc acttatgata gaaattaaag cgttgtcctc acgcatcagt atttagtaat
     9001 ttcgccttgc ggcattgcct taagcaaact tctgccactt catctcttaa taattttatt
     9061 aaaacatctt tctatatttc acttcgcatg ttgattcatc attattagtt attatttgta
     9121 cacccagcac atttccttgc aacacaagta gtttgaattt ttcacaagta taatataatg
     9181 taccgtctga aatttggtct acagaaatat cgcctaaaat atccagcact gtaaattctt
     9241 caaatactga tagttgttcc gcatatcgta cacaaagtct taccacactc tccgattgac
     9301 agttcattgc catcccacct atttatgctt tatttttaaa taatttaggg aaacatcgtt
     9361 caaaaaatct aggcgcaatt tgatacattt tcaacgcatg atgcatccat ttaggccgat
     9421 taatttccaa ttgttttgtt ttaatgccat aaatgatatc ttctgcaagc tgattagcat
     9481 caagcataat ttcccccatc tttttagcat acttcattga tgggtcggct ttttgatgaa
     9541 aaggtgtatc aatcgggcca acattaactg tcatgatatg taagtttggt gactctagtc
     9601 ttaaagcatt cattaatgca taaaaccctg ctttcgatgc cccataatgt gcagcatttg
     9661 cttgtgtgga aaatgcagct tgacttgaaa tacctacaat atgtgcgtta gatgttaaat
     9721 atggtctcaa cacagtatat aaaacattaa aactaattaa attaagctga tacgtttcaa
     9781 tcatttctga aaaactatgg tctgaaatag atttgaaata acctaaacct gcactataaa
     9841 tgaatccatc gaatgatgta ttgtcttcaa attgcagtgc ctgtatcgac ttcaaatcat
     9901 ttaagtcaca aggaataaca tttatagttt tccccaattc ctgttcaaag attctagttg
     9961 ctttatcaac atcacgcacc aacaacgtta catgcacttt attttctagt aactttcgga
    10021 caatcgataa acctaaacca ctcgtaccac cagtcactat aaaatgttgt cctttcatca
    10081 attaaccttc cttttcaatt atatagaatg caatttatca actttacata attgagacaa
    10141 gttgattatc tttcctaata tatatacaat aataagaaaa tataacatac aaatcaaaaa
    10201 ctaaagggat gtgacgttaa tgaaactcgt attttatgga gctggtaata tggcacaagc
    10261 tatatttaca ggaattatta actcaagcaa cttagatgcc aatgatatat atttaacaaa
    10321 taaatctaat gaacaagctt taaaagcatt cgctgaaaaa ctaggtgtta actatagtta
    10381 tgatgatgcg acattattaa aagatgcaga ttatgtattt ttaggtacca aaccacatga
    10441 ttttgatgct ctagcaacac gcatcaaacc acatattaca aaagacaatt gcttcatttc
    10501 aattatggca ggtattccga ttgattatat taaacaacaa ttagaatgcc aaaatccagt
    10561 tgctagaatt atgccaaaca caaatgcgca agttggacac tctgttactg gcattagttt
    10621 ttcaaacaac tttgacccta aatctaaaga tgaaattaac gatttagtta aagcatttgg
    10681 ttctgtaatt gaagtatcag aagatcattt acatcaagta acagctatca ccggaagcgg
    10741 cccagcattt ttatatcatg tattcgagca atatgttaaa gctggtacga aacttggtct
    10801 agaaaaagaa caagttgaag aatctatacg caaccttatt ataggtacaa gtaagatgat
    10861 tgaacgttca gatttgagca tggctcaatt aagaaaaaat attacctcta aaggtggtac
    10921 gacacaagct ggccttgata cattgtcaca atatgattta gtatctattt tcgaagattg
    10981 tctaaacgct gccgtcgacc gtagtattga actttctaat atagaagacc aataaaaaca
    11041 aacccgccaa cacatgtatg catcatcgca agcactgtgt ttgacgggtt atttttataa
    11101 tttattgtta tttggcaagc attgtttatt actttgtcat tagattttaa aactatcaaa
    11161 atcttttaca aaattaaaat taggtgtatc ttcattttgt atcaatgttt gataaatttc
    11221 atttatatct tctgtattat agcgattgct caaatgtgta atcaacgtac gtttaacatt
    11281 ggcttctttt atcaatgcaa atacgtcttc aatatggcta tgatgataat tgttggctaa
    11341 atgcttttca ccatctatat aggtcgcttc atgtaccatc acatcagcat ctctagaaat
    11401 cacacgttca ttagaacatg gttttgtatc accaaaaatt gctacaactg gaccctgttt
    11461 ggactcacct ctaaaatctt ttgattgata aacttgacca ttatgttcaa atgtatcatg
    11521 agattttact tcttgatatt taggacctgg ttcaagacca atgtttttta acgcttcaac
    11581 attgattgta cctgtagttt caggtgccat tactctatat ccatatgatg gaataccatg
    11641 attaagtaaa tgcgcctcta cagtaaaacc atcatgatga tatgtcagat gatcatcgat
    11701 ttcaatatat gtaattggat agtttaaatg tgactctgat aaattcatag acatttccac
    11761 atatgcttta attccttttg gtccaaccaa tgtaagcggc ttctgttcac cgccctgaaa
    11821 agaacgacta gaaagtaatc ctggcaaacc aaaaatatga tcgccatgca tatgagtaat
    11881 aaatatatgt gtcacttttc ctaatttaat tgcatgatgt aaaatttggt gctgtgtacc
    11941 ttcaccaacg tcgaaaagcc atatggaatt ggaatatggt tctaaattta aggcgattgc
    12001 ttgtgtattt ctctcttttg taggcaaacc tgcactcgtt ccaaaaaatg taacttccat
    12061 atatgcccct ccttttcttc aattcatttt atcataaaat ttgtatcaat ttagttttaa
    12121 tttatacttg aatcaaaatc tgttaaaaat tcgtctttgc tagttaattt ttctaacttt
    12181 aacgtagaca taactatata aattttgata attacgttat acttatcatt aataagtatc
    12241 acattaaaca tgatacatga atcgatattt catttaagac actgcataca gtcgagcata
    12301 ttgtatgacc tactgaatgg attatcttat aataataaat catatatcta attaagaatt
    12361 gaggttttaa tcttgagtac taaaaacaaa cacatcccat gtttaatcac aatctttggt
    12421 gcaactggtg acttaagcca tcgtaagttg tttccatcaa tattccatct ctaccaacaa
    12481 gacaatttag atgaacatat tgccatcatc ggtattggac gtcgtgacat tactaatgat
    12541 gatttccgta atcaagtaaa atcatcaatt caaaagcacg taaaagatac aaacaaaatt
    12601 gacgcgttta tggaacatgt cttctatcat agacatgatg ttagtaatga agaaagctat
    12661 caagaattac tagattttag taatgaatta gatagccaat ttgaattaaa aggtaatcga
    12721 ctattctatt tagcaatggc accacaattc tttggcgtta tttctgatta tctaaaatct
    12781 tctggtctta ctgatacaaa aggatttaaa cgccttgtta tcgaaaaacc attcggtagt
    12841 gatttaaaat cagccgaagc attaaacaat caaattcgta aatcatttaa agaagaagaa
    12901 atttatcgta ttgaccacta tttaggaaaa gacatggttc aaaatatcga ggtattacgt
    12961 tttgcgaatg cgatgtttga accattatgg aataacaaat atatttcaaa catccaagtt
    13021 acatcttctg aaatactagg tgttgaagat cgtggtggtt attatgaatc aagtggcgcg
    13081 ctaaaagata tggtgcaaaa ccacatgtta caaatggttg cattattagc tatggaagca
    13141 cctattagtt taaatagtga agatatccgt gctgagaaag taaaagtact taaatcactg
    13201 cgtcatttcc aatctgaaga tgttaaaaag aactttgttc gtggtcaata tggcgaaggc
    13261 tatatcgatg gtaaacaagt taaagcatac cgtgatgaag atcgcgttgc agatgactct
    13321 aacacaccta cctttgtttc aggtaaatta acaattgata actttagatg ggctggtgta
    13381 ccattctata ttcgtactgg taaacgtatg aaatctaaaa caattcaagt tgtcgttgaa
    13441 tttaaagaag taccaatgaa cttatactat gaaactgata aactgttaga ttcaaaccta
    13501 ttagtaatca atatccaacc taatgaaggt gtatctttac atctaaatgc taagaaaaat
    13561 acacaaggta tcgaaacaga acctgtccaa ttgtcttact caatgagcgc tcaagataaa
    13621 atgaatactg tagatgcata tgaaaatcta ttatttgatt gtcttaaagg tgatgccact
    13681 aacttcacgc actgggaaga attaaaatca acatggaaat ttgttgatgc aattcaagat
    13741 gaatggaata tggttgatcc agaattccct aactatgaat caggtactaa tggtccatta
    13801 gaaagtgatt tactacttgc tcgtgatggt aaccattggt gggacgatat tcaataattg
    13861 aattaaaacg cacatgttaa acaaaaataa atgagcgaat gacaaggatt gattacgaat
    13921 caagtaaatc aattgttcta tcattcgctt tttttctaga ctatattatg aaattatatt
    13981 ttacaatgcc caaaactatt ttaataatca ttgaacaaat gggtgtataa tttatagaaa
    14041 taatgtagaa taaaaataaa tgattgaatt aattggagtg aaagttttgg acgttatcaa
    14101 gcaaatacaa caggcaattg tttatattga agatcgttta ttagagcctt tcaatttgca
    14161 agaattaagt gattacgttg gtctttcgcc ataccatctt gatcaatcat ttaaaatgat
    14221 tgtcggctta tctccagaag cttatgcacg cgcgcgtaaa atgacactcg ctgcaaatga
    14281 tgtgattaat ggtgctacac gacttgtaga tatcgctaaa aaatatcact atgcaaattc
    14341 aaatgatttt gcaaatgatt ttagtgattt tcacggcgta tcacctattc aagcctctac
    14401 taaaaaagat gaattacaaa ttcaagagcg attatatatc aaattatcaa ctactgagag
    14461 agcaccttat ccatacagat tagaagagac agatgatatt tcattggttg gatatgcacg
    14521 atttatagac actaagtatt tgtcacatcc ttttaatgtt ccggattttt tagaagactt
    14581 gctcattgat ggtaaaatta aagagttacg acgatataat gacgttagtc catttgaact
    14641 atttgttatt agttgtcctc ttgaaaatgg tttagaaata tttgtaggtg taccaagtga
    14701 acgttatcct gcacacttag aaagtcgatt tttacctggc aaacattgtg cgaaattcaa
    14761 tttacaaggt gaaattgatt atgcaactaa tgaagcttgg tactatattg aatcaagttt
    14821 gcagttaaca ttgccatatg aacgaaatga tttatatgtt gaagtgtacc ctctcgatat
    14881 ttcatttaat gacccattca ctaaaattca gctttggatt cctgttaaac agagtcctta
    14941 tgacgaagat taaataataa aaaacaaaga agccccctaa tatatctata ggtctacaaa
    15001 tggccttaga ttctattagg gggcatatta atatgttaat ttagttcgat aacacatgct
    15061 tcatatggac gtaactgttt taaattaact ttggcatcat aattaaatag ctttacttct
    15121 ccatggctta aatcaaatgg tacagttaat tctgcttcgt ggttagtaag attacctaca
    15181 ataagaactt gcttttcatt taatgttctc gtgtacgcaa aaacttgtga attttcagca
    15241 tctactaaat caaattgacc atatacgtat acatcattag actttcttaa ttgaattaaa
    15301 tctttataaa attgtaatac tgaatgctca tcttctaatt gttgtgcaac attgatagtt
    15361 ttataattcg gattcactgg gaaccacggt tcaccatttg taaatcctcc atttaacgta
    15421 tcatcccatt gcattggtgt gcgagaatta tctcggttct catctttata tttcgcaagt
    15481 aaagcgtcta catctccacc ttgagctttc actatttgat agtcattttt aacagcaaca
    15541 tcgttaaacg tttcaatact ttcaaatgga taattcgtca taccaatttc ttgaccttga
    15601 taaatgaatg gcgtaccttg ttgcaagaaa taaacagctg catgacttgt tgctgattca
    15661 taccaatact tgtcatcgtc accccacgtc gatacacgtc gtggttggtc gtgattttca
    15721 ataaacaacg cattccaacc tttattttca agttgttttt gccatctatt taatacagat
    15781 ttatacgaat ttacatcaaa gtgagaatca ccactattcc acagtcccaa atgttcaaat
    15841 tggaatatca tattaaattt accattttct tccccgaccc agtcatcagc atcatcaggg
    15901 cttacaccat tcgcttcacc aacagtcata atgtcatact tacttaatga gcgatctttc
    15961 atctcttgta accaagtttg tatacctggc tgattcatat ctacatcaaa tgctggggca
    16021 tatgttttac cctcaggtac aggtaagtca cccgcttcaa acgtcttctt aatatgcgta
    16081 attgcatcta ctctaaatcc atcaatgcct ttatcaaacc accagttcat catttcaaat
    16141 acagcatctc taacttccgg attaccccaa ttcaaatcag gttgtttttt actgaataaa
    16201 tggaaataat attgctcagt attagcatca tattcccatg tagatccatt aaatatactt
    16261 tcccagttgt taggttcaga gccatctggc tttggatctt gccaaatgta ccaatcacgt
    16321 ttgggattgt ctttactaga tttggattct ataaaccaag gatgttcatc agatgtatga
    16381 tttacaacta aatctaaaat aagcttcatg cctctatcat gaacaccttt taataaacga
    16441 tcaaagtctt ccatcgttcc aaattcatcc ataatctctt ggtagtcact aatatcataa
    16501 ccattgtcat cattaggtga tttaaacatt ggactgagcc aaatgacatc gataccgaaa
    16561 tcttttaagt agtccaattt atcaatcatt ccaggtaaat ccccaatacc atcgtgatta
    16621 ctatcattaa aacttcttgg atatacttga tatgctactg cttctttcca ccattgctta
    16681 ttcattttaa aactcctttg ctatcgctgt gttgattttc ttatttttaa ttctgtatct
    16741 ataatgacga gttcaataac atcctgtgct ttgtttttca atatatttaa aattgctgca
    16801 ccagcctgtt gacctaacat tcgaggcttg atgtcaatac aggtttgtgg tggtgacgca
    16861 atttcggtta aataagaatc attgaacgtt gctgtcatta catctttcgg aatttcaata
    16921 ttaagttcat ataggacact taaaatcgct aaatgtaaca tagcatctaa cgaaatgatt
    16981 gcctgtttaa tatttgggtc cttcaaacgc gtatgtagat tttgcatgta attaaaaata
    17041 acttctcttt cattactagt ctcaataatt tgataattaa ttttattttg agaagctatc
    17101 gtttcaaatc cttgaattct atcttttgaa acttcaaaat ttcctttttc tgtaataaat
    17161 attaattcat ctacaccttg ttcaataaca tgtcgtgtca aattttcaga agctaatata
    17221 ttatcattat ctatatgtgt aaattgatga tctatatccg atgtaggctt accaatcaca
    17281 ataaatggca tgctttcatc aattaacatt tgtttaatcg gatcattttc ttttgaatag
    17341 agcagtataa acgcatcaac cattcgttgt ttaatcattt tataaacttc atccattaaa
    17401 tcattcatat tatttgagac tgtcgtttgt gtaccatagc catgctggtt acacgtttca
    17461 gaaattccta gcaatacatt gatgtagaat ggattcagtc gaataggctc ctcagaccct
    17521 tttaacacta aaccaatttt atatgtttgg tttgtaatta aagttctagc agcggtatta
    17581 ggaaaataat tcaattcttc cataactttc ttcacttttg aaattgtcgc ttcgctaata
    17641 cgttgatttc cttttataac tcttgaaact gtcgaaggag aaacaccggc ttttagtgca
    17701 acatctttaa tcgtaaccat ttaatcacct cctgttaatt tctgcattgg aaaacgcttc
    17761 caaccactgt ataataccag tttagtcaca ctttctaaaa aagtcaaaag atttgtgcaa
    17821 acgattgcat aaaacgataa aaataaaacc ttcatactga aattcaatcc gaaaatcaat
    17881 ataaaggttt gtataaatat taaaatcgat tgtttagtca ctaactgcaa aatagttacc
    17941 ttggccatct tgaaaattaa atacacgttg accattcatt tctactatat catgcccagt
    18001 taaacctaaa tcatttaatt ttgagtataa tgcatcaaag tttttctctt taaacattaa
    18061 agatggtgtt cctaggttca cttccgggct atgcttttca ataaattctt ttgccataat
    18121 cgtcaatgac gtttcagcat ctttggtagg tgatacttca actgcaacat agtcctcagc
    18181 taacggtgtt tcacttacaa caacaaattc taaagtttct gtccaaaatg ctttcgcttt
    18241 ttcgacatca tcaacatata acataacttg atttaacttt tccataaaat agtacctcta
    18301 tttctctata gtacatgcta tcataacaca gtaaatattt tattacttca caaaatgctt
    18361 aaaaatatgg cgggatgctt ttaaggtcaa ggataatact tgtgtaattt tttataggtt
    18421 gtagctactc tatcacactc tcttttatat ttatcaaaag atataaaaaa ggatagtatc
    18481 tttcaactat cctttaatca atattattct tcaatccatt gtgtatggaa tacgccttct
    18541 ttatcttttc tttcgtacgt atgagcaccg aagtagtcac gttgtgcttg aattaagttt
    18601 gcaggtaaat cagcagcacg gtaactatca tagtaattaa tacttgatga gaaaccaggt
    18661 gttggtacac cattttgaac accagttgcg acaacatcac gtaacgcatc ttgatattca
    18721 gtaacgatgt ttttaaagta aggatctagc aataagtttt gtaatcctgg attattatcg
    18781 taagcatctt tgatcttttg taagaattgt gcacggataa tgcaaccttc tctccaaatc
    18841 atagctaaat caccaagttt taaattccat tcattatctt cacttgcttt acgcatttgc
    18901 gcgaaacctt gtgcataaga acaaatttta ctcatatata atgctttacg aattttttct
    18961 aaaaagtctt tcttgtcacc atcaaatgat gcttttggac catttaattc tttagaagca
    19021 tttacgcgct cttctttgat tgaagagata aaacgtgcaa atacagattc agtaatgatt
    19081 gttaatggaa tacctaattc taatgcgtta attgaagtcc attttcctgt acctttttga
    19141 cctgcagtat caagaatttt ttcaactaat gcttctttat tttcatctaa tttcatgaaa
    19201 atatcaccag tgatttcaat taaataactt tctaattcac cagcattcca gtctttgaac
    19261 gtttgagcaa tgtcttcatg agacatgcct aataattctt tcatcatagc ataactttct
    19321 gcaattaatt gcatgtcagc atattcgata ccattgtgta ccattttcac atagtgtcca
    19381 gcaccattag gtccaatata agtaacacat gaagcaccgt cttttgcctt tgcagcaatt
    19441 gcatcaagaa tatctgcaac tttgttataa gcttcttctt gtccacccgg cattaatgac
    19501 ggaccagtta acgctccaat ttcaccacca gaaacgccca taccaataaa gttgattgca
    19561 ctttgtgcta atgctttatt acgtctgata gtatcttgat agtttgtatt accaccatca
    19621 attaaaatat ctccatcatc taataaaggt aacaaactat caatcgttgc gtccgtagct
    19681 ttacctgctt gaaccattaa taaaatttta cgtggttttt ctaaagaatt aacaaattct
    19741 tccaatgaat acgttggatg aatatttttc ccttttgatt cttcaaccat taaatcagtt
    19801 ttttcacttg agcggttaaa tacagataca ctatatccgc gtgattcaat attccaagct
    19861 aggtttttac ccataacggc taaaccaata actccaattt gttgtgtcat attacttacc
    19921 tcacttgttg atttttcatt agtattgtat cacaaaatag acatacacta cactaaatca
    19981 tttcgaatgt cgcgcaacta ttttgattat ttctaacact tgacttgcaa gcaagttcaa
    20041 tgatttaatc ggcattctct catttgttgt atggattttt tcataaccca ctcctaaaat
    20101 gactgaagga ataccaaatg tattaataat actgccgtct gaaccgccac cagaaataat
    20161 tgtatttgca gataatccta aattacgagc actttcttgt gcaattttaa caaccgcttc
    20221 attatcatta attttaaatc ctggataact ttgctccact gtaactactg ctttcccacc
    20281 taattctgat gcagtagttt caaacacatc agtcatatgt ttgacttgtg tttttattct
    20341 ttctggatcg tgagaacgtg cctctgcttc taaaatgact tcatctgcaa caatattcgt
    20401 agctgaaccg ccatgaaact taccaatatt ggcagtagtt atttcatcaa cttgtcctaa
    20461 tttcattcga ctaattgctt tcgccgcaat attaatagca ctaacaccct cttttggcgt
    20521 acttgcatga gccgttttgc caaaaatttt agctgaaatt aacatttgcg tcggtgcacc
    20581 tacaaccgta gtaccgacat cagcacttgc atcaatagca taaccaaagt ccgcgtccaa
    20641 caactctgaa tttaattctt tagcaccaat taaacctgat tcttctccaa cagtaatcac
    20701 aaattgaatt tgtccatgtg ggatttgttg ttcctttatc acttgcaaaa cttcaagcat
    20761 cgctgctaat cctgctttat catctgcacc tagaatagtc gtaccatcag agtatatgta
    20821 gccgtcatct tttacaattg gctttacatt aattgcgggt acaacagtat ccatatggct
    20881 cgtcaaatat aatttaggta cttcgccttc ttcgatagta ctattcattg tacacactag
    20941 attattggca cctaatttag gatgtttagc cgcttcatct tctttaacat ctaaccctaa
    21001 tgctatgaat ttttctttta aaataggttg gattgttgat tcattccctg tctcagaatc
    21061 gatttgtaca agttcaaaaa acgtattaag taatctttgc tcattaatca taatggaacc
    21121 ccttttatca aaattttcat gtacgccaaa cggtaaaaat tgatgaaata aaatgttaca
    21181 gtaattgacg ttacacagat ttatcaggtt tgtaaattgt gtcatattat tttcaattta
    21241 ttatatataa ttattgtaac tcaaactaag ctttgtcaaa aatatattga ttgatttttc
    21301 aaagatatcg tataatgagg aaaatgacat aagcaaactt actcatgttt tttattatat
    21361 tcctttatga tgattgctag ttatatcgtc tcaagttaaa agttttatat cttatgtcgt
    21421 aattattaat acaaaggtta ttcatttgga ggcacacaaa atgcaaaata aagttttaag
    21481 aattatcatt atcgttatgc ttgtatcagt tgtattagca ttgttattaa cgagtatcat
    21541 tccaatttta taaactatat ctcaactacc tatacaaaat catacaatta aaaatccatc
    21601 cattataaac gcatgtatta ataagttatc gtattgcaac gattactttc aaacatggct
    21661 catacggatg gattattttt taagctactt cactatgcat tttcaatgaa ccaaattgcg
    21721 atttgatttg taaatattct tctaattcat ttaatatttg aataatactt gctctcgagt
    21781 taagcgcttt gtgtgttgtt ggcaatggca gttcatccaa tttcaaacgc gtctcataca
    21841 aattgtgtaa acgcattgct gtatagtcat tactattcac atttagacca atttctttca
    21901 gcagtgacgc aacatcattt aaaagcggat ctttatgaca gatactttcg atgagcggtt
    21961 tcattctcat taacaattcc acttgctctt ctcgcatatc aaaataatga tagtatgaat
    22021 tttcgtttct aacaaaatga tttttaacat ctcggaacgc gatagacttc gcctttttaa
    22081 tatttaaaag taacacttca aattcaatcg caatggtatc ttcatatttt tcacaaatat
    22141 aactatattt actaaaaata tcagcaattt gttgctcaat tttacatttg tattcgtcta
    22201 gttgtttgtc taaacttggc atcattaaat tcattgtaaa tgcaatgctt agtccaatta
    22261 acagtaataa tgtttcatta acaattaaat gtgcatcaat tgattttgca ttaaaaacat
    22321 gaagtaatat aacgcaactc gtaatgacac cttcttgtac ttttaatacg acagttaatg
    22381 gtataaataa caatacgata ataccgagta caattggact ctgacctaat aaactaaata
    22441 ttgctgaacc taaaaacaat actaaaaaac atgatactaa tcttgaaata atcgcttgta
    22501 gcgaatgtac ttttgtatgt ttaatacata atacgactaa tatggcgctt gaagcataat
    22561 tatctaaacc taacagctta ctaataatta cacctaaagt catacccact gctgttttta
    22621 ttgttctaaa tccaatcttg taaggattta actttaacat gggttagcgc ctcttatctt
    22681 tcttcacaat atttattgaa taatgtttgt aattgattaa ttacgttcat cacatcatga
    22741 ccttcgattt gatgtctttc aatcatttct gtaatctttc catcttttac taatgcaaat
    22801 gacggacttg aaggcgcata accttcgaag tattcacgcg ctctttgtgt cgcttcttta
    22861 tcttgtccag caaatactgt cactagacga tcaggtaata cgtcataatg taaagcatgt
    22921 gatgctgctg gtcttgcgat accacctgca caaccacata cagaattgat cataactagt
    22981 gttgtaccat cttgtttaag aactttgtca acatcttctg cagtagttaa ttgctcatat
    23041 cccgcagatt caatttcatt ccttgcttgt tctacaacac cgttcatgta taaatcgaaa
    23101 ttcatgtcca taagttcaat cacctatccc tttatattta aactatcctc attctactaa
    23161 ttaataacat attgttcaat aaactaatct gaatcacacc tatatttaga cacaatttta
    23221 acaatatacc aaacattatt gtgcttaaaa tcatggtaac taatttgttc acatgttttc
    23281 attaatatgt ttcaagtatg atgtcttatt ttgactttac tgcaaaaatg cattcaacca
    23341 tgttgattat tgttctttat cttttttgaa tatattgcac atattttagt gccaaaaaat
    23401 aatacatcca tcgacaagaa caagataaaa caagttgtcg atagatgcat ctatgttatc
    23461 actaatatat atttgtattt tctaaagtat actgttcgat acgctgttta atatgattca
    23521 taaatttacc tgtttgtaaa ccatctaaaa tacgatgatc aattgaaata cataaattaa
    23581 ccatgttacg aattgcaatc atatcattaa ttactactgg ctttttaatg attgattcta
    23641 cttgtaaaat cgctgcttgt ggatgattta taatacccat tgatgatact gaaccaaatg
    23701 taccagtatt atttaccgta aatgtaccgc cctgcatatc ttcagctgtc aattgcttat
    23761 tacgcgcttt cgttgctaaa gtattaattt ctctagctat acctttgatt gacttttcgt
    23821 ctgcatgctt aatcacaggt acgtataatt tattttcatc agcaacagca attgaaatat
    23881 taatgtcttt atgtaagaca atttcatttc cttgccagct actatttaat aaaggatatg
    23941 cttttaaagc atctgctaca gcttttacaa agaaagcaaa gaacgttaga ttatatcctt
    24001 ctttattttt aaagctgttt ttataatgat ttctcgtatt cacaagattt gtagcatcta
    24061 cttcaatcat catccatgca tgtggaatct ctgttacact attaaccata ttttgcgcaa
    24121 ttgctttacg cacaccattt actggtattg tgctgttttc actattgtct tcagatgatt
    24181 ggttacttga tgtatctact gatgttgatt ttgtttgaac ttgtttgtca gattgagctg
    24241 tggtaccacc attttcaata actgacatta tatccttctt agttacacga ccttcaaatc
    24301 cactacctac aacttgtgat aaatcaatgt catgctctga agcgagttta aatacaacag
    24361 gtgaaaagcg accattatta cgtggttgat tttgtttagc agtagatgtc tgttccactg
    24421 ttgcactagc ttttttagta gatttctgag tatgctcatc cacttttgct tgtatctctt
    24481 cagttgtttc atttgtcttt tcatcagcag tttcaatttt acagataatt gtatcaatag
    24541 ctactgtctg ccccgcttca actaaaattt ctgtaattgt tcctgatatc gtggaaggga
    24601 cttcagctgt cactttatct gtaataactt cacataatgg ttcatattca tcaatatgat
    24661 caccaacaga aactaaccat tgttcaatgg tgccttcatg aacactctca cctaacttag
    24721 gcattgttat ttccatgact ttccctccct agaattctgc taattcacgc attttattta
    24781 agattttttc tggattcatc ataatttcat tttctaatac aggagaaaat ggcatagatg
    24841 gtacatctgg agcagctaaa cgcatgattg gtgcatctaa atcgaacaag caatgctctg
    24901 caataatcgc tgacacttct gacataatac taccttctaa attatcttca gttacaagta
    24961 aaactttacc tgtatgttta gcacgatcaa taattgtttc tttatctaat ggataaacag
    25021 ttcgtaaatc aacgacttca acattgatac cgtctgcagc taaaatatcc gctgcttgta
    25081 aacaataatt gaccattaat ccataacaaa atactgttaa atcttcacct tcacgtttca
    25141 catctgcttt tcctaaaggt acagtgtaat attcttctgg cacttcttcc tttaagaaac
    25201 gataagcttt tttatgctca aagtacaata ctggatcatt tgattcgata gatgataata
    25261 aaagcccttt agcatcatac ggtgtggaag gaataacaat tgttaaacct ggcgatgaag
    25321 caaatatact ttcaatactt tgtgaatgat atagtcctcc gtgaacaccg ccaccaaatg
    25381 gtgcacgaat cgttaatggg cattgccaat cattatttga acgataacgc attttcgcag
    25441 cttcactaat aatttgattt gtcgcaggta aaataaaatc tgcaaattga atttctgcaa
    25501 ttggtctttt acctaccata gctgcaccaa tggcagttcc aacaatattt gactcagcta
    25561 atggcgtatc gataactctg tcttcaccat atttttgttg cagtccttga gtagtaccaa
    25621 atacgccacc ttttctacca acatcttcac caagaataaa cacatcttta ttttgttgta
    25681 atgctaagtc ttgtgcctgg cgtatcgcct ctaaataaga taatttagcc attagttaag
    25741 actcccttct tcgtacacaa atgcataggc ttcttcgaca cttggatatg gcgcgtcttc
    25801 agcagccttt gtcgctttat tgatgatgtc tttatgctcc gcttctattt ctgccaacca
    25861 agcatcatcg ataatgccag ctgaaagcaa ctcttttttg aacttttcat tgcagtctgc
    25921 ttttttaagc gtttcacgct cttctttcgt acgatattgg tcgtcatcat ctgatgaatg
    25981 agctgtcata cgacttgtta ctgcttcaat caaagttgaa ccttgaccag aaatagctcg
    26041 atctcttgct tctttcatcg ctttatacat tgctaatgga tcattaccat ctacttgttc
    26101 accatgtata ccgtaaccaa gtgctctatc cgataatttt tcagctgcgt attgtaatga
    26161 atcaggtact gaaattgcat atttattatt tataatgaca catacaaaag gaagtttgtg
    26221 tacacccgcg aagtttaaac cttcatggaa gtcaccttgg tttgagctac cttcaccaac
    26281 agttgctgtt gcaattttct tcttaccatc catttttaaa gctaaagcag caccaacagc
    26341 atggggtatt tgagttgcta ccggtgaact ttgagacaaa atattcttag ctctactact
    26401 aaagtgtgat ggcatttgtt ttccaccaga gttaacatcg tctttctttc caaacgctga
    26461 taaaaacgta tcatacgctg agatacccat ataagtaacg aaagctagat ctctataata
    26521 aggcgctgta atatcacctt cttctaatgc gtatgccatc ccaatctgag ttgcttcttg
    26581 tccttgacca cttacaacaa atggaatttt acctgcacgg ttcaataacc acagtctttc
    26641 atctattttt ctacctaaat ccatccattt atatattact tttaggtctt cttcgctaag
    26701 gcctaatgat ttataatcaa tcatgttaaa tcctcctatt tatacgtgaa tagctctact
    26761 ttctgctttc aatcctaatt ccatcaacac ttcagagatg gaaggatgtg cgtgtgttgt
    26821 tagtcctaat tctaatgccg agccattcat gaactgtaac agtgatgcct cattaatcaa
    26881 ttctgttaca tgtggaccaa tcatattaat acccacaatt tcttcagttg attgatcaat
    26941 caccatttcg ctataccctt cgtttgtgtc atggctatca atcactgctt taccaattgc
    27001 tttaaatggt actttaaaac ttttaacttt cattccctct gcctttgctt gttcaatgtt
    27061 taaaccgata gaagcaattt caggttgtga ataaatacac ttaggcatca tgttatagtt
    27121 tactgggatt gggttcccct caaacatatg atcaacagcc acaacacctt cttttgatcc
    27181 aacatgtgcc aattgtaatt ttcctataca atcaccagct gcataaatat gtttatcttc
    27241 agtttgttga aattcgttcg ttaaaatatg tcctgatgtt gaaagtttta ttttagtgtt
    27301 gtttaaacca atatctgatg tgttaggttt tctaccaatc gatagcaaca ctttatctac
    27361 tttaattatg tctgaggaaa tttcaaacgt aacaccatct tcgttaacat ttatatcatt
    27421 ttcagaaagt tttattccct catagaattt aacaccacgt gctgacaatg atttttttaa
    27481 tagttgtgaa gcttgtttac tttcagttgg taaaattctt tcacctgctt ctataactgt
    27541 tacgtcaaca cctaaatcta tcatcaatga tgcaaattcc attccgataa caccaccacc
    27601 aataatacca atacttgatg gtaacgtctt taatgataat atatcatcgc tagataaaat
    27661 tttatcatga tcaaatgata agaatggcaa ctctgcaggc gaagaaccag ttgcaattaa
    27721 tacaaattgg ttgggtaata agtctgattc accatcttca tattcgacag aaattgtgcc
    27781 actttgaggt gaaaatatag atgtacctag aatacgtccc gtgccattat aaatgtcaat
    27841 gtgattgtgt tgcattaaat gctttacacc ttgatacatt tgattaataa tgtcttcttt
    27901 tcgtgccaac atattttcaa aattaacatt agcatctttg acatcaacgc caaacattgc
    27961 tgcctgtttt actgtttgaa atacttcagc agatttaagc agcgatttag taggaataca
    28021 acctttatgg agacaagtac ctcctaatag ttgtcgttcc actattgcca cttttttacc
    28081 taattgagac gcacgtatcg cagcaacata tcctgcagta cctccaccga gaacgactaa
    28141 atcatattgt ttctctgaca tgttcttact cctaactaat gatatatatc cattgaaaat
    28201 ttattaatac atagttttca tgtccattaa ttacctattt tacatgattg tctatttagt
    28261 ttgaatgcac ataaataaat ccataaatga gtattcaaca cactttggaa attatctacg
    28321 tctttgattg tgttgtatca tttctcttgc attttctcga gttaaatcag taacacttgc
    28381 acctgaaatc attcgtgcaa tttcatctac tttatcatcg ctaattaact cttgaacttg
    28441 tgttgttgta cgatcatctt ttgatgattt cgaaattaat aaatgatggt cgctcatcga
    28501 tgcaacttgt ggtaagtgag agatacaaat aacttgtata tattctgcta tatctcgcat
    28561 tttctctgcc attttttgtg cagcttgtcc agatacacct gagtcaacct catcgaataa
    28621 aattgcagtt tgacctctcg atttaacaaa aatacttttt aacgctaaca taattctaga
    28681 aagttctcca cctgacgcaa ttttatttaa actttttaat ggttcccctt tatttggact
    28741 gattaaaaat tctacaaatt cgattccatc aatattcggt tcttctaatt ttttaaatga
    28801 aatctcaaga tttgcgtctt tcatttgtaa gttttgaatt tccgatacaa tgtggtctct
    28861 taactcccta gcgactatac gacgctgctt tgataacgct tgtccaacct ctataacttg
    28921 attatacaat gcatttattt cttctcgtaa ctgcgatgta ctttgttcat agttttcaat
    28981 tttattgatt tcattattaa gcttttcttg atatgcgatt aattctgaaa tatcttttcc
    29041 atatttacgt tttaaattat tcagcaaatt catacgagat tcatactcgt ttaacacttg
    29101 ttcgtcgaat tctgtattag ccatttcatc atataactca tgttttgcat cttctaaaat
    29161 gtagtaaaat tgatcaatat cttcttttaa tttgtcatat ttgtttggaa ctatatcgtt
    29221 tattgttaac aaatggttgc ttagttcata taaacgatca gtgatagcat tttcatccgt
    29281 taatgtcata tgtgcgttat taagcgctaa gcttaatttt tcagagtttt gaatgcgttt
    29341 aatatctatt tcaagttgct ctatttcgcc ttcttttaga tgtgcttcag acaattcttc
    29401 taattggaat ttcattaaat ctaaacgctg tagcaatgct tggtctgctg attctaaatc
    29461 ttctaactct tgctttttgg ctttataatt ttgaaaagtt tggtgatatt tatccaacaa
    29521 atcttgataa cgtgattctg cgtaattatc caataatgtt aaatggtatt tttgtttcaa
    29581 caaagactgc gtttcatgtt ggccatgaat atctaataat tcttgcataa cttttcgtaa
    29641 atcttgtaaa gtaactgttt gattattaat tttacaaaga cttttaccag agctgaaaat
    29701 ttcccgttta acta
//
LOCUS       DAKTGS010000014        29458 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.014, whole genome shotgun sequence.
ACCESSION   DAKTGS010000014 DAKTGS010000000
VERSION     DAKTGS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.014"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            416..2380
                     /locus_tag="O0286_000546"
     CDS             416..2380
                     /locus_tag="O0286_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCY0168945.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            2724..3803
                     /locus_tag="O0286_000547"
     CDS             2724..3803
                     /locus_tag="O0286_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168946.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(3908..4501)
                     /locus_tag="O0286_000548"
     CDS             complement(3908..4501)
                     /locus_tag="O0286_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0168947.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(4523..5491)
                     /locus_tag="O0286_000549"
     CDS             complement(4523..5491)
                     /locus_tag="O0286_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCY0168948.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(5569..6003)
                     /locus_tag="O0286_000550"
     CDS             complement(5569..6003)
                     /locus_tag="O0286_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0168949.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            6230..6514
                     /locus_tag="O0286_000551"
     CDS             6230..6514
                     /locus_tag="O0286_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0168950.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            6685..7491
                     /locus_tag="O0286_000552"
     CDS             6685..7491
                     /locus_tag="O0286_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0168951.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(7611..8282)
                     /locus_tag="O0286_000553"
     CDS             complement(7611..8282)
                     /locus_tag="O0286_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0168952.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            8552..9544
                     /locus_tag="O0286_000554"
     CDS             8552..9544
                     /locus_tag="O0286_000554"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0168953.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(9793..10593)
                     /locus_tag="O0286_000555"
     CDS             complement(9793..10593)
                     /locus_tag="O0286_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0168954.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            10914..11549
                     /locus_tag="O0286_000556"
     CDS             10914..11549
                     /locus_tag="O0286_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0168955.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            11555..12286
                     /locus_tag="O0286_000557"
     CDS             11555..12286
                     /locus_tag="O0286_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCY0168956.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(12446..13066)
                     /gene="srtA"
                     /locus_tag="O0286_000558"
     CDS             complement(12446..13066)
                     /gene="srtA"
                     /locus_tag="O0286_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCY0168957.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            13260..13751
                     /locus_tag="O0286_000559"
     CDS             13260..13751
                     /locus_tag="O0286_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0168958.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
     gene            complement(14189..14359)
                     /locus_tag="O0286_000560"
     CDS             complement(14189..14359)
                     /locus_tag="O0286_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168959.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(14421..14519)
                     /locus_tag="O0286_000561"
     CDS             complement(14421..14519)
                     /locus_tag="O0286_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168960.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(14538..15437)
                     /gene="sdaAA"
                     /locus_tag="O0286_000562"
     CDS             complement(14538..15437)
                     /gene="sdaAA"
                     /locus_tag="O0286_000562"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCY0168961.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(15451..16131)
                     /gene="sdaAB"
                     /locus_tag="O0286_000563"
     CDS             complement(15451..16131)
                     /gene="sdaAB"
                     /locus_tag="O0286_000563"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCY0168962.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(16134..17177)
                     /locus_tag="O0286_000564"
     CDS             complement(16134..17177)
                     /locus_tag="O0286_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCY0168963.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            17728..17931
                     /locus_tag="O0286_000565"
     CDS             17728..17931
                     /locus_tag="O0286_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168964.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            18114..19049
                     /locus_tag="O0286_000566"
     CDS             18114..19049
                     /locus_tag="O0286_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCY0168965.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(19131..20099)
                     /locus_tag="O0286_000567"
     CDS             complement(19131..20099)
                     /locus_tag="O0286_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0168966.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(20232..20576)
                     /locus_tag="O0286_000568"
     CDS             complement(20232..20576)
                     /locus_tag="O0286_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0168967.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(20618..21028)
                     /locus_tag="O0286_000569"
     CDS             complement(20618..21028)
                     /locus_tag="O0286_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0168968.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(21408..23474)
                     /locus_tag="O0286_000570"
     CDS             complement(21408..23474)
                     /locus_tag="O0286_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0168969.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(23775..25514)
                     /locus_tag="O0286_000571"
     CDS             complement(23775..25514)
                     /locus_tag="O0286_000571"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCY0168970.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(25561..26250)
                     /locus_tag="O0286_000572"
     CDS             complement(25561..26250)
                     /locus_tag="O0286_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCY0168971.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(26243..26638)
                     /gene="cidA"
                     /locus_tag="O0286_000573"
     CDS             complement(26243..26638)
                     /gene="cidA"
                     /locus_tag="O0286_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCY0168972.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(26851..27729)
                     /locus_tag="O0286_000574"
     CDS             complement(26851..27729)
                     /locus_tag="O0286_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0168973.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            27896..28114
                     /locus_tag="O0286_000575"
     CDS             27896..28114
                     /locus_tag="O0286_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0168974.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            28503..28934
                     /locus_tag="O0286_000576"
     CDS             28503..28934
                     /locus_tag="O0286_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0168975.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(29199..29303)
                     /locus_tag="O0286_000577"
     CDS             complement(29199..29303)
                     /locus_tag="O0286_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168976.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
BASE COUNT         9347 a         5447 c         4424 g        10240 t
ORIGIN      
        1 cttaatactt gtagtattta ttttcaagaa gaaaaatatt tagtaactta aagtattaaa
       61 tgtctaaata cacacatatt ccatcgtaat tcaaaatcat tttcaaatcc cttcacccaa
      121 ataatggtgc ggggattttt tcatccaaat tttggaaatt ccacatttta catatcgtaa
      181 ttttttagga aactagtgaa tataacaaat ccctcctctc atttttaaaa tagatatatc
      241 acttccccac ttttacttaa ctaaactgca acggttccta ataccaaaat cctgccctct
      301 atttttatca attcaagcat acttattgaa aaatgttaac gttttcttga taatcattgt
      361 aagcgcattt attttataaa ctaacgtttg aaatatacta caggagtgac acgtaatgac
      421 tcaaattact gaaaaagaat taaaaaagaa gtatttagat ttactatccc aaaattttga
      481 tactccagaa aaacttgcaa ctgaaattat caatttagaa tcaattttag aattacctaa
      541 aggtacggaa catttcgtca gcgatttaca tggtgaatat gaagctttcc aacacgtatt
      601 acgcaacggt tctgggaacg tgcgagcgaa aatcaatgat attttcaaag agagactttc
      661 aactaaggag cttaatgact taactgctct tgtctactat ccagaagaca aattaaaatt
      721 gattaaaagt gatttccaaa attgcggtca acttaatgtc tggtatatca caacaatcga
      781 acatttaatt gagttaatta aatattgttc ttcaaaatat acgcgttcaa aactgcgtaa
      841 agcattgcca aaacaatacg tttatattat tgaggaacta ctgtataaaa gtaatgaata
      901 tcaaaataaa aaatcatatt acgaaacact tgttaaccaa gtaattgaac ttaaacaggc
      961 agatgattta attattggac tcgcttattc cgtacaacgc ttagtcgtcg atcatttaca
     1021 cgttgtcggt gatatttatg atcgtggacc acaaccagat aaaattatgg atacactgat
     1081 taattatcat tccctagata ttcaatgggg taatcatgat gtgctttggg ttggagccta
     1141 tgctgggtca aaagtatgct tagcaaactt acttcgaatt tgtgcacgtt atgataattt
     1201 agatattatc gaagacgctt atggcattaa tttaagacca ctgcttactt tagctgaaaa
     1261 atactatgac gcagataatc ctgcttttaa gcctaaaaaa agacctgaca aacacgaacg
     1321 tttaactcaa cgtgaagaaa gtcaaattac taaaattcat caagctattg cgatgattca
     1381 attcaagtta gaaataccaa ttattaaacg tcgtccaaat ttcgaaatgg aagaacgtct
     1441 tgtgcttgaa aaggttaatt atgatacaaa tgaaattaca gtttatggta atacataccc
     1501 attgaaagac acatgtttcc aaactgtcaa tcgtgataat ccagcagaat tactacctga
     1561 agaagaagaa gtcatgaata aactattatt gtcattccaa caatctgaaa aattacgtcg
     1621 tcatatgtct ttcttgatgc gtaaaggctc tctttactta ccatataatg gcaatttact
     1681 cattcatggt tgtattccag ttgatgaaaa tggtgagatg gaatcatttg aaattgatgg
     1741 tcatacttac agcggccaag aattattaga tgtgtttgag tatcatgtcc gtaaatcatt
     1801 tgatgaaaaa gaaaatactg atgacttatc gacggattta gtttggtatt tatggactgg
     1861 gaaatattcg tcactatttg gtaaacgtgc catgactacg tttgagcgat actttattgc
     1921 agataaagct tctcataaag aagaaaagaa tccgtactat catcttcgtg aagatgtgaa
     1981 tatggttcgt aaaatgctca gtgatttcgg attaaatcca gatgaaggac gcattattaa
     2041 tggtcacaca ccagtgaaag aaatcaatgg cgaagatcct atcaaggctg atggaaagat
     2101 gcttgtcatt gatggtggct tttcaaaagc ttatcagtca acaactggca ttgcaggcta
     2161 tacactattg tataattcat tcggtatgca actcgttgca caccaacaat ttaacgctaa
     2221 agaaaaaata ctttccgaag gtatcgatga actttctata aagcgcgtcg tagataaaga
     2281 attacaacgt aagaagattc gtgataccaa tattggtaaa gatcttcaag ctcaaataga
     2341 tattttgaaa atgttaatgc atgatcgtta cttagactaa agacgacatt tgatgcatgt
     2401 atcgtgcact tatttagtgg tactgtgttt acgcattggc tacgcaaaca cgatgataaa
     2461 taggcgacct agctacaccg aaaaaaatcc cctcactatt gcgagtagtg aggggattgg
     2521 tgtatctatg ctaattcctg ttaactgtat tataacaact ttacagcaaa aaaatgcaaa
     2581 atcaactaat aaattaatga aatgaccgac accctcgctt ctactccaat ttaacaaaat
     2641 actatattag tactatattt ttcattaaca tgtatgttac attttctata ttacttaaaa
     2701 ttacgaaggg acatttttaa atcatgcgta tcttaaaaga gtccattatt gtggcatttg
     2761 cctttgttgg tgttgtcgtt ggtgccggct ttgctactgg tcaagaaatt ttccagtttt
     2821 tcacaagtca tggcgcatat agcatttcag gcattattgt aacaggacta ttgattactt
     2881 taggtggaat ggttgtcatg catacaggtc atcatctaaa gtccagaaat cattctgatt
     2941 caattaacta tttcttatac ccctctattg caagaggttt tgatattatt ttaacaatgt
     3001 ttatgttgtc tttagctatt attatgactg caggtggtgc gtcaaccatt catcaaagtt
     3061 tcaacttacc gtattggctg agcgcactca tattagtcgc ctttatttta gcaacactgt
     3121 ttctaaaatt cgatcgttta attgctgtgc ttggcggtgt taccccattt ttaattgcga
     3181 ttgtcattat gattgcggtc tactatttca caacaagtca tcttgatttt actgccgcta
     3241 ataatgatgc tcagattcat aagcagaaat cattatcacc tggatggtgg tttgatgcga
     3301 ttaactatgc aagcttgcaa attgctgctg ccttcagctt cttatcagtg atgggtagta
     3361 aagttaaata tcgtgactca acgttatacg ggggcttgat tggcggttta atcattacat
     3421 ttttactcat gatgattaat ctaggtttaa tttctcaatt cgataaaatt aaacacgtag
     3481 atctacctac attaaaatta gcgacacaaa tgtctccgtc aattggtatt attatgtctg
     3541 tcattatgat acttgtcatc tacaatactg ttgttggatt aatgtatgca tttgcgtcac
     3601 gtttcagcgt tccattcagc agacgttact tcatcattat tattacaatg gctgtcatca
     3661 cttatattag tacatttatc ggtttcattt cattaattgg aaaagtattc cctattatgg
     3721 gattgttcgg tttcatctta ctcatacctg tactctataa aggtttaatt aagcgtatta
     3781 ccggcaaatc tcatatcgat taaaatatct atactattgt aaaatttagt acagcacttc
     3841 aaaatcaaaa aagcgggcag atgaaaagta aaaatctttt ccatctgtcc gcttcttaat
     3901 agaactgtta tttcccaagt atttgttgac cagccgttaa tccagtttct gtaatttcat
     3961 ggcccttcac ccaaacttct tcgacttgtg ccccacgtgt attaaacaag ttaatgactt
     4021 gttcacttgc agctaatggc acaattggat catgtttccc catagaaagc aacacactga
     4081 catctgataa atcctttgtt gacgttactt caactgggta taacggtgca tataacaatg
     4141 cttttttaaa tggtgcttct gaacgcaaca ttaagttaat cgctatattt gatccatttg
     4201 aaaatccaac aagtactgct ttttcaatat caaaatcata acgttcagca gcttctttaa
     4261 tgaacgtcaa caattcttgt ccacgaaatg ccaaatcttc ttcatcataa acaccttcac
     4321 caagacgttt gaaataacgg ttcatcccat tttctgaaac ttgtcctcta atacttaaca
     4381 agtgataatt ttcattcaat gcttcgccta acggtaataa atcgaactca tcaccacctg
     4441 taccatgcaa taatattagt gttggcgcac cattttgtcc ttctctaaaa atatgttcca
     4501 tcacaaatcc tcctcattcc aattaaccat gttgacgctt cgtattaaaa ggtctaactt
     4561 ccgattcaat atattctctt ttattttcta aaaatggtgg taaggataac ccttcgccta
     4621 atgtttcata aggttcatct tccataaatc ctggtccatc tgttgaaatt tctattaaaa
     4681 tatgccccac acgtgcatat aatgcttcaa aatagaaacg attaacgatg cctgagttat
     4741 taatacctac ctctttatat ttcgttgccc acgcttctat tgcatcatga tctttcacac
     4801 gaaatgacac atgatgtacc tcaccataac cttgacgtgc tgctggccct ttatcatctt
     4861 ttattaagat tacctggcca ccattgcctc cttcgccaac ttcaagtaat gcgacattat
     4921 cttcatgcgc aatagttgtc ataccgtaaa cagtctctaa aatattttta aagtcgtcaa
     4981 aataacttac tttaatttca atggggccta atccataaat cgctttatct actggaaccg
     5041 gtccattctt ccaaggtaca ccaggtgcta ccccttcatt taactcatct gaaattaatt
     5101 gatacacttg gccatcgact tcttcaaatg gcaacacttt tttaccaaat aattcttgaa
     5161 taccttcgtg tttaacacca aactcatcaa agcgctgttc ataatatgtt aatgcgtcat
     5221 cgttaggcac tctaaaagac ggtcttgtaa tggaatttgt tcctgcctgc ccttttgtaa
     5281 tatttggaaa atcaaagaac gtcatgtctg tacctgccga acctacatca tctgcaaaaa
     5341 aagtatgata cgtataaata tcatcttgat tgactgtctt tttaactaaa cgcatgccta
     5401 gtacttctgt aaaaaattta taattacgtt ctgcatcatc tgtcattgca gtaacatggt
     5461 gaatacctag taattcatga ttatttatca ttttatatac cctctttata tttttagtgc
     5521 atttatctcg aaatcgagat actttaatgt aaatttttag tgcacgcctt acacttctgt
     5581 agattgtgca cttagtttct taaacgcttg ttgtaagatt gttaattcat cctttgttaa
     5641 cacatcaaac gcttttgtta atgtctcagc atgcttaggg aaaatatctg ccatttgact
     5701 ttgacctttt tcagttaaac aagccatata tacacgttta tcatctttat ccttttcacg
     5761 tgtaatccaa cctttgtcct ctaattgact tacaacatat gaaatgctgc tacttgcaat
     5821 taatacgcgg tctctaatac gttgaattgg ttgcggacct ttattataaa gcaactcgag
     5881 cactgcaaat tcagtaatat ttaagccata tcgctttacg tcttcttttg taatttgctc
     5941 taatgtgtct aacgccctat tcattccgac aaaaacattg agagattgtt tcgttcgatc
     6001 catatgatca ccaacttttt tattttattt cgatttcgag ataaattata tagctgaata
     6061 gatttaatgt caagatacgt gttttcatct tcatttcaaa ctgtgcttaa taaaaacgac
     6121 tacatagatg tggcaatgtc gtcacatgac tgtcatgcac ctttgacttc gtttaagtta
     6181 aaatagaata gggaaatact ataatcaatc atgataggag ttgatatgta tgagtaacct
     6241 tgaaatcaaa caaggcgaga acaaattcta tattggtgat gatgaaaata atgctttagc
     6301 tgaaatcaca taccgttttg tggataataa tgaaattaac attgatcata caggcgtatc
     6361 tgatgaactt ggtggtcaag gtgttggcaa aaaactactt aaagcagttg ttgaacacgc
     6421 tcgagaaaat aatttgaaaa ttattgcctc atgttcattt gccaaacata tgttagaaaa
     6481 agaagattca tatcaagatg tatatcttgg ttaaataaat tttgcgatgg tgcaactgta
     6541 ccatcgcttt tttgttcgca tcaatataaa attgcaaaac gactagttat tttgcatttc
     6601 aaactttatg ctatcaattc attttctttt ctggtatact ttttatacta acttatagaa
     6661 aaatagaaaa agaggcgatg cataatggca tttcacgata aaacagcaac acaagtgaca
     6721 aacattgtac tgaacgtaag agacttagac ttaatgacaa cattttacaa aaacatttta
     6781 ggactatccg ttaaaagttc tgacgataat acaaccgtat tatctgttgg gactggtggc
     6841 catactctaa cgttacattt attagaagac ggccgtcaga cttccccacg tgaagcaggg
     6901 ctttttcata tagcattttt attaccaact actgaggatc tagctaactt cttatatttc
     6961 gtggcacaaa aaaatatggg catcggcgct ggtgatcatt tagtaagtga agctttatat
     7021 ttcaacgacc ccgaaggtaa tggtattgaa gtctatcgcg atagaccttc atcttcatgg
     7081 gaatggcaaa atggcaaagt taaaatggat actttagaag ttgatagcca aaccttatta
     7141 acacatcgta ctgatgaagg ttggcaagga atgccagcaa aaggcatgat tggacactta
     7201 catttaaaaa cacatgattt agacgcagca tatcaatgtt acattgaaca attaggattc
     7261 caacatgtgt ctgattttcc acgtgcgcta tttatgtcaa cgaatcatta tcaccatcat
     7321 attgctgcta atacttggca atccaatcaa gcaagacaaa acaattcaca aagttatgga
     7381 ttaacacatg ttgatattta tcaacccgac gcctcaacac atacatttac ggcaccagaa
     7441 gggtttgaca tcacagttca ttcaaatact gaccttgttc cagaaaaata atcatccata
     7501 ctttattaat acaatttttt cacgcaaaag cctgcgattc atttatgtaa tcgcaggctt
     7561 ttttattgct aactttttaa ttttatatat ttagacatac ggtattctat ttattctttt
     7621 ggtccaaccc attcaataac atcttcataa gcttggcgtg ttttattttt cggtggctct
     7681 tgttgtctgt agccaaatgc gaccatcact gataaaccaa attgctcagt atctaagatc
     7741 cctttatttg ctaaaatgtc tgtcactgta tccagactaa aaccttccat cggacatgaa
     7801 tcaataccta acaatgcggc tgtcgtcatc atattgccta atgcaatata cgtttgttta
     7861 cttgaccagt catacaaggc ttgatcatta tcagaaatat ggaaatctgc ttggaatgca
     7921 tcgaattttt gttcaacagc tggaatcgtt tgtgcctcat attttttaat atctcttaac
     7981 atatgttgta catacggtga tcttgacgtt acatttttac gcgcaaaaat tagcacaaaa
     8041 tggctcgctg tatctaattg ttttgctgcg ccccaactgt gcgcttttaa ttcatcacgt
     8101 aacgcttgat cttgaatcac gacaaacttc caaggttcta acccaagaga acttggagac
     8161 aatctacctg actctaatat tgtttcaaaa tcttctttcg aaactttctt ttgtggatcg
     8221 aattgcttag tcgcatgtct gaaatgaaat gcatccataa ttgtttgatt catattgctc
     8281 attgaacata gcctcccatt tttatattta ttgctataca aattatactt catgttttaa
     8341 aaagactaaa gtaatccgtt tgcaattcac tataacttgt gcacatgtta taagttttct
     8401 gcaataaaac acacttttga attttgggaa atttagtgtt ataatatatt tgtgatattt
     8461 ttcacaatta aattgtggcg tgattttaat gtgcataatc tttaatttca ctcatttact
     8521 tttcaatctt ttaaaagcga ggtttttaat aatgacaaaa attatgttct ttggtacgcg
     8581 tgattatgag aaagagatgg cattaaattg ggggaaaaag aataatgtcg aagtaactac
     8641 ttctaaagag ctattatcaa gtgctacagt cgatcaatta aaagattacg atggcgtaac
     8701 tacaatgcaa tttggtaagt tagaaaatga cgtttatcct aaattagaat cttacggtat
     8761 taaacaaatt gcacaacgta ctgctggatt tgatatgtat gatttagatt tagctaaaaa
     8821 acacaatatt gtgatatcta acgttcctag ttattcacct gaaacaattg cagagtattc
     8881 tgtttctatc gccctacaat tagtgcgtcg cttcccagat attgaacgcc gtgtacaagc
     8941 acatgatttt acttggcaag cagaaatcat gtctaaacca gttaaaaata tgactgttgc
     9001 aattattggt acgggtcgta tcggtgctgc tacagctaaa atatatgcag gatttggtgc
     9061 tacaattaca gcttatgacg cctatcctaa taaagattta gactttttaa cttataaaga
     9121 tagtgttaaa gaagctatta aagatgccga tattatttct ttacatgttc cagcgaacaa
     9181 agaaagctat catttattcg ataaagcaat gtttgatcat gtgaaaaaag gtgcaatctt
     9241 agttaacgca gcacgtggtg cagtcatcaa tacacctgat ttaatcgctg cagtgaacga
     9301 tggtactttg ttaggtgctg cgattgatac ttatgaaaat gaagcagcat acttcacaaa
     9361 tgactggact aataaagaca ttgacgataa aacattatta gagttaatcg aacatgaaag
     9421 aattttagta acaccacata ttgctttctt ctctgatgaa gcagtacaaa accttgttga
     9481 aggtggttta aacgcagcat tatctgtcat taacactggt acatgtgaaa cacgtttaaa
     9541 ttaatatata tatttaaaag caaaggcgcg ccatgtgcct ttgcttttta ttttgtatat
     9601 ctgtatcaaa atcgatttga ttaaaatccg ctttatttta tcatctattc aaatgatttt
     9661 agtgcgctta ttttactatg gcattattgc ctaacctgtt tgagatatat taatatttga
     9721 tgattcgtat tcaaagtttt catttaacat gtatttagta tcatgatagc tgcttcattg
     9781 atgatatact acctactttt tcaacacttc ttgttcgata atcgttaata caccctgctt
     9841 atcattactt ggagcgacag cagaggcaat attgaacaat tcttcatcat gactattttc
     9901 catcacataa ctatgcttag cgaacgctaa catatcttta tcattattcg catctccgaa
     9961 ggccatgagc tctgaaggag acatttccca tttatctaac aatcgtttta atgcctgccc
    10021 tttagtcatg tttggcataa taatatcaat gctatcgttc ccacttgaga caagtttaat
    10081 atcattgctg aattgcgttg ctacttcttc gtcaacattc ggatgcgtct cacgattaat
    10141 attaaatgct attttgacat aatcatcctc aggtaattgc tgtagactgt caatttcttt
    10201 taactgatga taataaaatc tcgtatcttc tttaaacgct tcagaagtat gttttaaaat
    10261 atacgcgctt ttcaaaccac agatgacgag ttgatcaata ctttgcttca tatttaagta
    10321 atcgacaacc tgttgaaaca cctgacgatt aaagctttta taattatata actcattgcc
    10381 attataaata actgcaccat tttcagaaat aaaatacata tctctatccc cgaaaataga
    10441 cttcaacttc gcatattgat tgccactcgc agcaataaat gtaatatctc tatttctaag
    10501 ttcagtaaaa atcgcttcaa atctaagttt atcatatgtc ttttttgaat ctaaaaacgt
    10561 cccgtccata tctaccgcaa tagccttaac catgattatc ctcctcattg ctcttttagt
    10621 cgtcttcatt atatccatct catgacaagc aatgaagttt gtcataccac aatgaaccta
    10681 tattcacatt ttgaaattgc tttcatatat gcaaaacaca ttacactgga tatagcattc
    10741 agaggcgaac ataaatttca gaatattgta aaaatatcac actatattta tcatttagac
    10801 aaaatatagt gtgatatact aatttataac atctatacat aatgtgaaag aatttttaaa
    10861 aatacaatta aatatcggtg taataaatcc ctttatacat aggagttgta tacatgaaac
    10921 taaataacta ttctttaaaa gttaaaaaca aacaactagt tgacaattgt gatttaaatt
    10981 tctatcttgg tcagatcaat cacattgttg gtaaaaatgg tgtaggaaaa tctttattag
    11041 ctaaagattt cttactaaat aatagtggaa atatccctaa gtccatttct caaaatgtaa
    11101 ccttaatatc aagttcatca aatattccta atgatataac aaaagatttt ttattatcat
    11161 tgttaaaatc aaaatttgaa aacaatcgac aaacattcga taagatttat aacatactaa
    11221 acatcgaagc aataccgtct aacgtactac taaaaaactt gagtgatggt caaaaacaaa
    11281 agcttaaatt attaagtttc ttattggaag atcatgattt aatcatatta gatgaagtta
    11341 caaacgcttt agacaagaaa acagttaatg aaatttatga atttttaaat gattttattc
    11401 aaagccatca aactaaaact attatcaata ttacacataa tttatccgat ttaagtgctt
    11461 tgccgggtaa atactttatt tttaaagacc ttcaaataga agagtaccaa tcaaaagaag
    11521 aagtcataaa tgattacatt aatttataat tgaggtgtcc actttgaaat tagaatttaa
    11581 aaaaagtatt tccaataaaa ttatttacac gcttgttgtg ctattcacat tccttttctt
    11641 acttgggtat tttttaccaa taggtataga caaagttaaa agtttaagct atagccagtt
    11701 tttcttcagt tcatatactg ttgcaacgca attagggttc ttactatttt catttgttat
    11761 tgcatatttt attaataaag agtattcgaa taaaaatatc ctgttttata aattgattgg
    11821 cgataatatc tttaccttct tctataaaaa ggtagcagtg ttattttttg aatgtttagt
    11881 gttcataatt ctaagtatca ctattatttc aattatatat tcggatttct cacattactt
    11941 actattaata atcttatttt cactcgttat cttacaatat atcttagttg ttggaacgat
    12001 tagtatggtt tcacctaaca ttttaatcag cttaggtatc agcattgtat attggattgg
    12061 ttcagtcatc ttagttgcca ttaataaaaa tatatttggt atcgttgctc catttgaggc
    12121 gagcaatacg atgtatcgtg ccgtagaaaa aatattgaat aacgagtcaa catttatgtg
    12181 tcctactgaa attataaata tagtatcatt cttcgttctt ctatttatag ttaatactat
    12241 tgtcttatta ctaagtagaa agagatggtt gaaaatcggt atgtagtata aaaaaggagc
    12301 ggtatacatt gccgcttctt tttattatgt atttttacaa tctccaccaa tgacacgtcg
    12361 tcattgctac ctcataccaa acaaaaaaca gtctcgcaat caagactgtt ttccactcaa
    12421 tatattcatc cattagcgta atagattatt tgacttctgt agctacaaag attttacgtt
    12481 tttcccaaac gcctgtcttt tcattgtaat catcacaagt aattaatgtt aattgtttat
    12541 ctttaccttt ttgttcatct agaactccta catctgtagg cttaacatct cttatacttg
    12601 tcattttata cttacgtgtt tcattaccaa ctttaaagta caccatacta ccttttttgg
    12661 ctgctttaag atttgtaaat tgatagttcg gacggtcaat gaaagtgtgt cctgcaattg
    12721 aaatattttg atcatctagt gattcatttt cttctgcaaa gcttacacct ctatttaatt
    12781 gttcaggtgt tgctggtcct ggatatactg gttctttaat atcagcatct ggaatttcaa
    12841 tatagcctgc cactttcgat ttatctttcg gaatttgagg tttagcttgc tgctttttat
    12901 ctttactcgc ctgttctttt acatttttat catattgttc aatcttttca tctttatctt
    12961 tatcgtgaag ataattatcg atatgtggtt tagcaaacaa atatgctgcc actaggataa
    13021 gtaccacacc agcgattgtc attaatcgat ttgtccattt tttcatacgt taaggctcct
    13081 tttatacatt tcacaatgcc tgttaacatt tcatatagta taccattaat ttcaaaatga
    13141 ctcatagcaa ttcattttat actataaaat ttacatgtat acttttacgt tagatttcat
    13201 tacacatatt tgcattcaaa taacgaaacg cttttaataa ttactaaggg ggaattgata
    13261 tgattagata cgctaaaaaa gaggatttaa acgctatatt agcgatatac aatgatgcca
    13321 ttatcaatac tacagctgtt tatacttatg aaccacaaac catagacgaa cgtgtcgcat
    13381 ggtttgaaac gaaacaacgt aagcatgagc ctatctttgt atttgaggaa aatggaagtg
    13441 tcttagggtt tgcgacgttc ggttcattta gaccttggcc agcataccta tatacaatcg
    13501 aacattctat ttatgtcgat gcttcagcta gaggaaaagg tattgctagt caattactac
    13561 accatttaat tgtggaagca aaagctaaag gttatcgtgc gctagttgca ggcattgatg
    13621 cttccaacaa agcgagtatt cagttgcatc aaaagtttgc ttttaagcat gccggcacac
    13681 tgaccaatgt aggttttaaa tttaatagat ggttagattt agcattttac gaattagatt
    13741 tacaagacta gtaatgtttg aatcacataa tataaacaag acaaccatgt taattccctt
    13801 aacataacaa gccaacatat aaaattttaa acttctcagg ggagtgggac agaaatgata
    13861 aagagccact aatgatttat tatgtagtgg ttcttacaca ttagccacag ctaatgtgta
    13921 cttaaaaata ggaatacatg agtaaaactc atgcataaga aatactaatt tctatagaaa
    13981 aagtatttct ttatcgtcgt cccaccccaa ctcgcacatt attgtaagct gacttttcgt
    14041 cagcttctgt gttggggccc aaaaagcttg ttacaagcgc attttcgttc agtcaactac
    14101 tgccaatata actttgtaga gcatattaca ttgatttaca ttgtcccttt tatttattct
    14161 tttcaaatac tatccccata gctttgattt aacgcttttt ctcaataaca aaacgaatat
    14221 agtagaacat gaaaacgata atcatgctga gcgataaaga tttaaataat agattgaccc
    14281 acgttccctc agtcgtatat ccatatgtaa tcgttgtgtt aatgatgaat gctataaaga
    14341 tgattgatag tcttagcata tcatcactcc ttttaagtta ttttagatat acgggggcgc
    14401 ttttgcaatc actattttga ttagtatgca ttttccataa atctttcaac ttcttcagag
    14461 ataattaaga agcatctatc tggtactaat gatccagaca gatgcttctt ttttatcaat
    14521 attttattgt tatctcatta attattttta accatatctt cagctgtgcc aaagatttta
    14581 cgtttaattg cttcgccagt tggtgtgcct gctagtccac ccaatccagt ttcacgtaat
    14641 gatgcaggaa ggttacgacc aaccttatcc attgcttcaa taacttcatc aacagggatt
    14701 ctactttcaa tacctgctaa tgctaaatct gctgaaatta aagcgttacc cgaaccaatt
    14761 gcatttctca taacacaagg aatttcaaca agtccggcta ctggatcaca aactaaacct
    14821 aataaattac ttatcgctaa tgccatagcg tgcccggatg cttctggtga tcctccgaat
    14881 atagctactg ctgcagctgc ggccattgca gatgctgaac caacttcagc ttggcagcca
    14941 cctgttgcac cagctacact tgcattgttt gctacgacac gcccaaacaa tgctgaagtg
    15001 aataagaaat caatcatttg ctcttctgtt aaatcatgtg ttttttctaa tttaaaaagt
    15061 gcaccgggaa tggtacccga ggaaccagct gttggcgttg cacaaataat acccatcgca
    15121 gcattgactt catttgttgc aatggcacct ttgactgcgt caatcatttc atatccagac
    15181 aaagcatgat gtgtttcatt ataatcacgt agtttagcag catcatgacc agtgtagccc
    15241 gttacacttt caaccccatc acctgtcgtc cctttgatta ctgcgtctcg catgacatct
    15301 aaattttgtt tcatttgcgc tcgcacttca tcacgtgatt taccgcttaa ttccatttct
    15361 tctttaacca tgatatccgc aaatgacata ttattttcta cggcataatc tatagtctct
    15421 ctaattgaat caaacatgtt tattccccct ctaatttata taggaaacgt ttacgtcact
    15481 gtatttctct ttaattgtat ttaatgttga ttctgagatt gctttattta atggtattac
    15541 aaccaagcat ttatcttcat ctatcttaat aaattcatct ttacagtcta atttcatatc
    15601 gttgatatca ttgatgaaat gatttacttg tgctttagtc atatttccgt caacaactaa
    15661 aattggtaat ccatgattta aatctacttc tagtccattt atatgaatac ctttaatttt
    15721 aattgtacca ccaccgattg aaataccgat aatttcaatg tagcgaccat cattacgaga
    15781 tgatttgata taagcacagt ttggatgttg accaatacta tcgccttctt cttcgatgat
    15841 atctatttta ataccatcat cagctgcaat ttctaatgaa gatttaattc ggttatcaaa
    15901 tgttgaatat cccattgctc cacccacaat agcgacatct gtaccatgtc cttggtgtgt
    15961 ttgagcaaat gattcataat aatgtatttc aatattttta ggaatatctc ccaatattgc
    16021 gcgtgctgaa ttcccaatct ttactgcacc agccgtatga gaacttgaag ggcccatcat
    16081 aactggtccg ataatatcga aagcactttg ataatcatag ctctttgcca taattaaaca
    16141 ctctccttaa tatgattctt tttgctcggc attttaaagt tgatattcat taaattaaac
    16201 ttattaatta gtgttccaat aatataggct aagacaatgc tgacaataat cactgttgcg
    16261 attgttacaa ttgatgtcac tgcattatta aagccaaaca atacgatggc gcctgcaatt
    16321 ggtgttgcca tacctttgac acctattact agtccgctaa atgtcacgat acatgcgttg
    16381 acgaccccaa tcagtgcatt tgtaccatat agttgtactg gatattgcgc tattaaatca
    16441 atttgcgtca atggctcaat acaaactgca aatgcttttg acggtccacc aatgtttaat
    16501 tttcggaata aaataaggtt aacaaatgag ctacctgtac atgttagtgc tccaatagcc
    16561 ataggaacac ctgtcagtcc taataaactt gttaatacca ttgaacttag cggtgtcata
    16621 cctgtaacag gaatcactag tcctaaaatg accgctaatg catatggatt gttatcacct
    16681 accgcagtga cagcactacc tatttgtttt aatgttgcta gcacaccagg tgtaatgatt
    16741 gatgcaagtc cgaaagcaat tgctggtgca aataagatca ccacaattaa gtccaagcct
    16801 tctggaactt tcttttcaat ccatttaatt aaaaaagcta cgccataagc tgcgatgaat
    16861 gctggtaata atttaaagtc atgtaatact aaaccaacaa tgaccgcaaa tactggtgca
    16921 acgcctaagt ttaagcacgt tagaatacct actgcgatac cgcttaaact tcctgctaaa
    16981 tccccaatat cttgtagaaa tttaatatca aatacgccac caatagcata acttaagaat
    17041 gcttgtggta gaaatgtcgc acaagctgca ccggataatg cttgtagtcc ttgtttaccg
    17101 tacggtgcat actttaaaaa tagcgtcatg atcactaaaa ccaagactaa tgtgcctaca
    17161 cctaacagaa tatccatttc ccaaaaacct ctctctatgt ttattttatt ttcagaccat
    17221 aaacatcgta cacacttaag aaaacgtttt caacttttat ctgttatcaa atcaaatatt
    17281 taagtgaaat atttgatatt tgtgaaagat ttttaaaatg gattgtttca aaaactactt
    17341 atgttgtcgt taatatttac taattaactt tttactctat atttcaaaca gttgtgtgac
    17401 agttttttga taactttttt acatctgaaa gtaagtaata tttctaaaaa cttttaatat
    17461 ttatacactt tatctttcga gctatttatt ttttgttttt gtgctgtgtt tataaaaccg
    17521 cacactatat tcaaaaataa cttattacac atatatttgc aataagataa ttaaagtagg
    17581 atattatttt tagttttctg ataggaatga tgatagtcta taggttgaat ctttactttt
    17641 ttttaaagct aaatttacat caacttaaca atggttggtt atactgaaga tgaaaatatt
    17701 tagtataact tagtggaggc gataaaggtg caattgagtc attccgttaa agttgcaatt
    17761 tctatctatt tagcacttat ctttataacg ttcacttctt atttagtcat tattttatat
    17821 acgagtatga ctggacatga tgtatcacat ttcgtgttag atagtcagca ttctcatcat
    17881 ggatctctta cgcaaaaaca tttgagtctt cctgaaatct catttaaata gtttatcctc
    17941 tgtgtttcaa cattcatttc ccatatcgat tcatttatct atcatctaga ccactacatc
    18001 ttagatgatt tttttatttt ctcatttcac tctttcttaa aagtcgatat aatgaattaa
    18061 atcattatca tacaccgaca tattttatgt tgttggtgtt aagttttaaa ggggtgagat
    18121 acttggcgaa taatcattca gctttgcgtt taaaaggaat tatacttgcc attgtcggtg
    18181 cttgtttatg gggattaggt ggtactgttt ctgatttctt gttcaaatat aagaatatta
    18241 atgtcgattg gtacgtcact gctcgacttg tagtcagtgg tgttttctta cttattatgt
    18301 acaaaatgat gcaacccaaa cgttcaatat ttagcgtatt ccaagatcga cgtatgttag
    18361 gcaaattact tatcttcagt atactgggca tgttagtagt acaatatgct tatatggcat
    18421 ctattaatac aggtaatgct gcgattgcaa cattactaca atacattgcg ccagtttata
    18481 ttattatttg gtttgtcata agaggcgttg caaaactaac attatttgat gtgcttgcta
    18541 ttatcatgac actattagga acatttttat tattaacaaa tggttcattt tctaatttag
    18601 tcgtcaatcc tgcaagttta ttctggggta ttttagctgg tgtagcactc gctttttaca
    18661 caatttatcc ttcagaccta cttaaccgct tcggttcgat tctaattgtc gggtgggcaa
    18721 tgcttatttc tggtgttgcg atgaatttac gccatccaat ttggcacatt gatatcacta
    18781 aatgggacat atcaattata ttatttttaa tctttggtat tatcggtggt accgcactcg
    18841 cattttattt ctttatcgac agtttacaat acatatcagc gaaagaaaca acattattcg
    18901 gaactgttga acctgtcgta gccgttatcg caagcagtct atggttacat gtggcattca
    18961 aaccatttca aatcgtaggc atcattctta ttatgatttt aattttatta ctatcactta
    19021 aaagacaacc tgaaacatta gatgaataag aaaactctga taatcacttt agcaagtaac
    19081 tattatttaa caacgtagtt accttatagg tgatatcaga gttttttatt ttagttaata
    19141 atatttttca cttggtataa aaagcgtcgt cgctctggta tcggaaatac tggataaata
    19201 tggaattggt aatatccagg tataaagtca tgttcgatac ctttcgcact caacaattgc
    19261 gataagttca aagcatctgg gtacagtact tcttttgtac caacagttaa tgtaatacga
    19321 cctaaaccat ctaagtcacc attaattggt gatactttgt agttatctaa aggtgtgtcc
    19381 cctgcccatt gttcagctaa gaacacactg ccatccacac ctaccattgg gtctttcttt
    19441 aagtagtcag gaatttcagg atgctgcatc gttgcatcta aaactggtga aattaataca
    19501 atatgtcctg gttgcacaat atgtttttct tttaacaatt gagcaaatga taaagcaatt
    19561 tgaccgcccg cagagtcacc cattacaacg atttgtttag aatctgctac ttgattcaat
    19621 aaatcatggt acaacttttc aaaaagcaca tacgtcgctt gataatcttg atgcggaatc
    19681 ttcggatata ctggcatgat gactttagca ttgagtgttt ctgcaagttc atcaataaat
    19741 tcgaaatgaa ttttgagtgg gtcttggaac catgcgcctc catgtgcata taatacaacg
    19801 cgttgatgct tatcttgttt atcgttaacg gtataaacgg ttgaaccgaa gtgcttatcc
    19861 acttttactg gcatcttaaa ctgataattg gttccctcat attcgcctgc tgtctgtggc
    19921 gccatttttt ctaatgcttt catcgcttct tctttataca taaaacgacg cttagctcta
    19981 gataatcgta taccttgttc taacatataa cttttcacac tgcgtttttc tttaattcta
    20041 atatgtgcgt atgctgcaac taaaaatcca aacgcaagtg tagaccattt tttacgcatt
    20101 tgtcgtcact cctctttgat gatttctact atagttctac tgtaccatgc tgattctaat
    20161 tactaccaat atttactggg aaataagtta tgatgtctac ataatcttag caatgctaat
    20221 catttgcgat attaatgctc atcgacatcg ttatttacac tatcattcat ttgcttaaga
    20281 tgatgttcaa aacgttgcat atcgataaaa cgcccttgac gatgcatttc ttttccattc
    20341 ataaaaatta aatccacagg tacagtgaaa atatttaatt ctccagcaat agcttccacc
    20401 tgactttgat taatcacagc taatggcaca ttgggatatg attgcatcaa gtcttcaatt
    20461 tgtggtaaaa cggcatgaca cacgctacac tgatcgcgca tgacatgtac aactgctaac
    20521 ggatattgat gaataaattc acggaatgct tcgacactat ttaattgctt aaccatttcg
    20581 aacaccttct tactttttat attttaaaat gaagtcatta ttttgttgct aaaatgtctt
    20641 gaaacacttg tggcacagag gttatcttct tatcactcaa tctgtaaaaa ccgcctgtct
    20701 gtgtaccaat acaatgcacg ccatgtgcgg acacaatttc aaactgaaac acccatttca
    20761 tcttgcttgc tttaactaag taaatacgac ctactggttt gtcatgtatt gtgacaggta
    20821 ctttatactc tgcttccgtt ttcataagta taggtgaaat gccttgattg atcattgttg
    20881 agtaattcat gtgctgatta ataaacgctg ttctcaaatc ttcgaaccat ctaatataaa
    20941 cgatattact aacgacaccc atcgcatcaa tgtcgtaacc attcacttcg atttctttct
    21001 ctgcaactaa tagaccttct gttgtcataa tgacaaatct ccttatctta caaatagatt
    21061 taaaatcatg ttaatttatt tttattactt tcatgataaa aatacaagca attgaacttt
    21121 attacggaaa atcaaacttt tcaatgagac tattaatttg gctagtcgcg aagatgcatt
    21181 tttctaaaat gtgacaaaaa cataagcaat gttttctata tctaacacta atatttaact
    21241 tattatcatc aacacacctg ctatacatca ttgcgcacta aaaaggcagt agcttcattt
    21301 taagctactg ccttttgtaa cgtattctct ttcaactcgc tacttcgcat acgcactacg
    21361 aatgactaaa agttcattca tttgatatgc tgtttcattt ttaatcatta tttgactgtc
    21421 ataattagct tacctggatc agcatcttgt acatcttcaa tgacaagttc tttattttca
    21481 agatttgtaa taatcattgg tgtaacgatg cttggtgcgt gtgctttcaa gtatgctaaa
    21541 ttcactttca ttaatggttg accttgtgtt actttttcat caacgttaat cagactttcg
    21601 aatccttcac cattcagttt cactgtatcg ataccaatat gaataagtac ttcgacgcca
    21661 ctttcagatt ctaatcctat cgcatgtttc gtagggaaga ttgttttcac tgtaccatca
    21721 aacggtgcta caatttcacc tttttcaggg ataaagccaa caccatcacc catcatttta
    21781 ccagcgaaca cttgatcagg tacttctgat aatggaatga tttgaccgat acctggtgca
    21841 tagatgtctg ttgttccaag tgcttctacg tgaactggtt catctgacat ttcttcagtc
    21901 actgtcgttt cacttggttt cgtaatttca ccactcataa tcttggccat atcatgttta
    21961 atttgatctg attttggacc aaagatagct tgcatattgt ttccaacttc taatacacct
    22021 gatgcgccta aagctttaat acctgctaca tctacttttg atttatcaac cacttctacg
    22081 cgtagacgtg taatacatgc atctaaatgt ttaatgtttt cttttccacc cattgcatct
    22141 aagacatcaa atggtaattt tgcgacacta gagttacgaa tttcagtttc ttcatcttca
    22201 cgacctggtg ttttcaattt aaacttacga attgcaaagt cgaataagaa gtaatacacg
    22261 atagcatata caataccgac tggaataact aataatgcgt gtgaacgatc ccagtttaat
    22321 aaaccatata aaatataatc tatgaaacca cctgagaatg tcataccaat tttaacgcct
    22381 aataaatgca ttactaagaa tgatgtacca gctaataata cgtgaattcc ataaagtact
    22441 ggtgctacaa ataagaatga aaattctaat ggctcagtga taccagttaa aaatgcagtt
    22501 aatcctgctg ataacattaa accacccacg acttttttac gttctggtcg tgcattttta
    22561 taaatagcaa atgccgccgc tggtaaacca aacatcataa atggatattt accagtagta
    22621 aatgcaccag cagtaaatgg tacgccatct ttcaattgtg ccatccaaat acgttggtca
    22681 ccacgaacca attcacctgc gtgatttgta taacttccga attcaaacca gaacggtgaa
    22741 tagaaaatat gatgtaaacc aaatggaatt aatgagcgtt caataatacc gaatataaac
    22801 gttgttaatg ttaaattttt atttaataag aaattcgata aactatttaa tccatcttga
    22861 attggtggcc acgcaaagct taaaagcaca cctgttgcga ttgctacgac cgatgtcaca
    22921 atcggtacaa atcgtttacc tgcaaagaat cctaaaaatg gtggtagtgt aatattataa
    22981 aatttgttgt aacaccatgc ggctaaagca cccatgataa taccaccaaa cacacccgtt
    23041 tgtaacgttg gaatacctaa tactaaagca tgtgctggtt ctttcgctgc ttgacttaat
    23101 tcttttgccc ctttggcata tgagaaaatg tcatcaattg taatgtgcaa cactttcccc
    23161 attgttgcat tcataattaa gtaacctact agcgctgcta atgctgcaac accgtctcct
    23221 cctgctaatc caagtgctgt accaactgca aataataatg gcaagttatc aaatacaact
    23281 tgtcctgctg cttccatgac cgacgaaatc attacaatga tatcgttttt taaccatggt
    23341 gcaatttcta ctaattgttc gttgtgcatt gcgttaccaa acgctaataa aataccagct
    23401 gctggtaaaa tcgcaacagg taacattaat gctttaccga tacgttgcaa ttgtccaaac
    23461 aatttcttaa acatataaac atcccctatc tcaatttccg cttccatgta caaaaaaagc
    23521 aggcatgaac aaacaagact attctcatag tgcggttgtt tgctcatgcc tgcattacca
    23581 gtaacacgca agtgatatct aattcatagc tattatacat atttgtaaaa gcgttttcaa
    23641 caactttttc acaattttat catcaattca gtacgatcat ttgtgataaa taaacaaaac
    23701 ggcaattccc attttgttga aatagaaatt accgttttta tagttcaatt caattgtgat
    23761 aacctttaaa tcagttataa gaaacgtttt gctgccactg aaattggtgg aatttgatct
    23821 aaatctaaat gtttatcttc agtaattgat ctaaatgccc acttaccata accaagcgct
    23881 tcttcattta caattttacc tggtaatggc gcagcattag gatcaacata tacatctaca
    23941 atcgttggta catcttgtgc taatgcctct tcgactatag catctacttc gctagcactc
    24001 ttaattgtat aacctttacc acctgctgcc tcagcaaatt ttgcatgatc catatcagaa
    24061 aaatcaactg catattctaa ttcaccagct gcttgttgtt catatttaat aaatgctaac
    24121 tgtttgttat taagtacaaa tacagttaaa ggtaaatcat attgtactgc tgtagcgaag
    24181 tcttgcatta ccatttggaa tgcaccgtca ccagcaattg cgatggcttg tctatttgga
    24241 tatgcaattt ttgatgcaat tgcacctgga agaccgcaac ccattgtacc taaccaactt
    24301 gaaatgatga acttgttatt tacaccaagg tttaagtatc gagttgacca aactgttgct
    24361 gtacctacat ctgctgaaat cactgcatca tctttaataa atttattgat tgatgccatt
    24421 aatcgttctg gacgtaatgg tttactatta ttatttttat cttgttccat ccatttatcc
    24481 caaaccgctt tacgttctaa cgttttgttt aagaatggtc tttcagcaac atgtttaata
    24541 ttttcagtta actgatgcaa cgcaatttta ctatctccaa caattcctac attaatattg
    24601 aaacgatgtc cgatattttt aggatttgtg tcaatttgaa tggctttaat atttttctta
    24661 ggtaagtaat ccacatatgg atagtttgta ccaaccataa ttaataaatc cgcttcctgc
    24721 attgtttgat aagatgtttt ggtaccgatt ttacctaagt taccgatact atatggatga
    24781 tcatccggca agattgtttt agctggtaat gaatgaatga caggaatttt agccatttca
    24841 ataaattcac gtagctcatc tttcgcatgt ttcgcaccta caccaattaa catgacaggc
    24901 tttttacttt tattaattag tttaaccgct tttttgatgt ctttatattt tggtgatact
    24961 actgttggtc ttgatgtatc tactggttta ttcgttgtat ctttaatttt ttcagttaat
    25021 aagtcgttag gacaaataac aacagctaca cctttttgtt catatgccgt acgaattgct
    25081 tcgttaacga tttcaaacac attgtcacct ttttcaattt ggtgattata aacggctaca
    25141 tcttcacata atttttgtaa atttgtttct tggaatgctt tcgttccaag tgctgtacta
    25201 ttcgtttgtc cagataatat taattgcggt acattatcca ttttggcatc atacatacca
    25261 tttaataaat gaattaaacc agggccaccg atacttaatg ccacaccgat tttaccagtt
    25321 aattttgtgt aaccagcagc cgctaagctt gctacttctt catgacgtac atgataaaat
    25381 ttaaattgat ctctcactgt acgtaaacta tcgactactg cgtcgattga gtctcctgga
    25441 ataccataca agtgatctat atcccatgct tgtaatgctt taactaatgc ttcatttgct
    25501 tttatttttg ccattattac taatagcctc cctttctgtc tatcatttta aatatctaaa
    25561 ttaatagaat aataaaatta gaacaggtat taacactgaa ctaataactg cagttaaaat
    25621 catcccaatt gaactaaatg caccggattc aatatccatt tctagtgctt tagctgtgcc
    25681 aaatgcatgt gacgcattcc catacgttaa tcctttggcg atagaagatt caaatcttcc
    25741 aaatcttaat aacatcgaac ctaaaatact accgattaaa cccgttgtga taataaaaag
    25801 tacggtcatc gtatctgtac cacctagttc atgtgacact tcgataccta ctgcggctgt
    25861 tatagatcgg ggcaataacg ttacaatgac gtctttagaa tagccaaatg ctttaagtgt
    25921 taagaacact aacatgaaat tcagcatgac gccagttaat acacttgcaa aaatgataga
    25981 gacattgtct ttaattttct ctctattttt ataaagtggg tacgctaaac atacgaccgt
    26041 tgcgtttaaa atatggttga tccaactgcc acctttcata tacccgttat aactaattcc
    26101 aaagataagt aagacaaaaa taattcctaa agatgcaatt aatgctggat tcaaaaatgg
    26161 gttcggatat ttttgttgta accttttagc gaaataatat aaaacgacag tcaacaaaat
    26221 cattaataag gcttgcacgt aatcattcat aagcgtctac acctttacga tgtttatgtt
    26281 taacagacat tttttcagca atataacctg aagataatgc aacgatacat gttcctatga
    26341 taatgactgc gaaaaagagt atataattta gcgtaatttc ggaagcaaca tccataatac
    26401 ctacaactga cggtatgaag aaaaagacca tcgtctttaa taaaaagttt gcaccgtctt
    26461 ctacccaagt tagcggtaca atcttaaatt gtaatagtaa ataaaataaa aatagaccaa
    26521 caatactgcc ggctaaggga agatgaaaaa tcttttgaat ttctgtgcca atataagtaa
    26581 tcacaatgat gattcctagt tgtagtagta gtttgattat taattggact ttgtgcatgg
    26641 cgccatccct ttctaaatat gtctaaattg ttacaataac tattataaag atggcgtaca
    26701 caatataaaa atactctttt tttatagttt gtattactat tttctatgga aacgctctct
    26761 aaatttcact tcattttcac aaaaaaacac tcattatttc aatgacccac gtaatgatgc
    26821 gccttaaata atgagtgctt tattaatatg ttagcctaaa cggtctttca aaaattctat
    26881 ccatttcgtt gtggcatgac ttaattgttt atccttcttc caaacaacac ctaattccca
    26941 atgtacatga gcatcttcaa tgcgtagcag cttcacatct ccatttagtt gctctgaaat
    27001 tgatgttggt aaaatactaa tacctaattc attcgtaact aaatcttcta taacatgcca
    27061 ttgtgaaatt tgcgctacag tattcggaac aaagccaacg tttttagcat tttcaataat
    27121 tttatcattc aagtaaaagt ctttattaaa taaaatgaag tcttcacctg ctaaatcttc
    27181 gagtttaaca gtttcatatt ttgcgagtcg atgctctctg ctcacgataa gtcgcaaatc
    27241 ttccttatct agggtagtat aatcgaaaat atgatgatcg actggcaaag tggtcacgcc
    27301 tatatctact tcatcattga taatttgctg ttcaattgtt ttaccgccat tttctatcaa
    27361 attatatgta acatttggat attgttgatg gaatgcacca agaatattga taagaatctt
    27421 catattcatg actgccgaca tgcccatgtt tatatgtcct gtttccagtc cattcaagcg
    27481 ttccatttca gatggtaaat aatcatacag tgcaacaatt tctttacttt tctcataaaa
    27541 aatttgacct gcatcggtaa gaattaaatg tcttttactt ctatcaaata agggcgtgcc
    27601 catttcattt tcaatatctt taattgcttt actaattgta ggttgtgcaa tatataatga
    27661 tttggaagca ttagtcatgc ctccttgctt aacgacttca ataaaatatt tcatatgttt
    27721 gatatccact aacatcatcc taactatttc aacacgttta tttcattata cacaacttct
    27781 taaacattca ataaatttag atttataaaa ttgctttata aaaagtttta tgtaaaatac
    27841 acattctttt atggtattat attaatggct caagtatatt aatcgaggtg aggtattgag
    27901 cttttacgaa tttatgcaaa gttttcttgg tgatgacaca ccattaggtg aattggttga
    27961 ttggattaat caagatagca atttccctag agaagtgaag agccatagtg aaatattgtc
    28021 atattttcga acaaatccat gtcctgaaag catctcagta cctgtaatta aacgggcact
    28081 atcagttttc aaccaattca caaatgtaca atgatacatg aatacatgga gtcacatcat
    28141 ctcatgttag cgctctgatt gcagggcgct atttttaatg ttattgctac atttagtata
    28201 ttgtgattgc tattttcttt cagttataga accatgtacc ttttttttaa atcattttat
    28261 attttcccac taatatccgc tgttaatcaa tcctgacatc cttgtatcac tatgacaatt
    28321 aattgttaaa tacatgaatt tctacatttt atgaaaaaat ccatttttat tacaattcaa
    28381 cactttatat gacaacttca ttacagttac ttttattgtt gattgcttac attgttttct
    28441 aaaaaaaatt tgttatcata attaacgttg aataaagaaa aaaattaact tgggagataa
    28501 aaatggaata taaaaagata ctaattcgtt tattaattgc ttttgcagta cttttctcag
    28561 cagatttcac ttatcaatcc gtcgaacaaa cgcatcaatc gcatgccgca gttaattatt
    28621 atagtaaaaa ccaatgtaca tggtgggcat ttaaacgtcg cgcacaagtc ggtaaacctg
    28681 tttctaatag atggggcaat gctaaaaatt ggtattacaa tgcacgtaaa tcaaaatatg
    28741 cgactggtcg tacaccaaga aaatttgctg tcatgcaatc aactgcagga tattatggac
    28801 atgtcgcagt tgttgaacaa gtatataaaa acggtagtat taaagtttca gaatacaact
    28861 tttatcgccc attaaaatac aatacacgtg tactaagcaa aaaggcagca cgtaacttta
    28921 actatattta ctaatcaaaa aacttctatc acgaacgctt caatttcctg tatgcgtgtg
    28981 atagaagttt ttattttatg aaattatatt attacttcta caaatttcaa attgccgtaa
    29041 ttgaacgtat atttcttctt caactattat ttcatcttta gcataatcta tatataaaat
    29101 tttatgctat tatttaaata attcgctata acttaacata cgttttcgat ataaaccttg
    29161 ttctaaatct caataatttt ttgctgtttt catcgtcatt agttaaaaaa ataatttaac
    29221 tgagtttttg agacctgttt aaattgatga ctcaaaacct ttatcccctt ttcactcggt
    29281 ttaattgctt taatatttaa cacagtctca ttgtaatttt gctactaagt ttgaaatatt
    29341 tcgattcgaa tgtgaaacat catttattat tactttcgac agctagaaaa atattgttaa
    29401 accaaaacta taattaccac ttttatatag aactatatat aaattttaac tttgagac
//
LOCUS       DAKTGS010000015        29226 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.015, whole genome shotgun sequence.
ACCESSION   DAKTGS010000015 DAKTGS010000000
VERSION     DAKTGS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.015"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..1163
                     /gene="fmtA"
                     /locus_tag="O0286_000578"
     CDS             <1..1163
                     /gene="fmtA"
                     /locus_tag="O0286_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCY0168977.1"
                     /translation="ACVLLFIIISIALIFHRLQTKTHSIDPIHKETKLSDNEKYLVDR
                     NKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKMSKGYGYQDFEK
                     GIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFKTSKPIPLKDLM
                     LHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLVLAKVIEEVTGK
                     SYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRPNILDQYYGAGN
                     LYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFYAKPTLNRLNGG
                     FFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTKGVIVQ"
     gene            complement(1700..1978)
                     /gene="qoxD"
                     /locus_tag="O0286_000579"
     CDS             complement(1700..1978)
                     /gene="qoxD"
                     /locus_tag="O0286_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCY0168978.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(1987..2592)
                     /gene="qoxC"
                     /locus_tag="O0286_000580"
     CDS             complement(1987..2592)
                     /gene="qoxC"
                     /locus_tag="O0286_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCY0168979.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2582..4570)
                     /gene="qoxB"
                     /locus_tag="O0286_000581"
     CDS             complement(2582..4570)
                     /gene="qoxB"
                     /locus_tag="O0286_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCY0168980.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4570..5670)
                     /gene="qoxA"
                     /locus_tag="O0286_000582"
     CDS             complement(4570..5670)
                     /gene="qoxA"
                     /locus_tag="O0286_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCY0168981.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            5972..6094
                     /locus_tag="O0286_000583"
     CDS             5972..6094
                     /locus_tag="O0286_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0168982.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(6242..6559)
                     /locus_tag="O0286_000584"
     CDS             complement(6242..6559)
                     /locus_tag="O0286_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCY0168983.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
     gene            complement(7364..8224)
                     /gene="folD"
                     /locus_tag="O0286_000585"
     CDS             complement(7364..8224)
                     /gene="folD"
                     /locus_tag="O0286_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCY0168984.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            8425..8907
                     /gene="purE"
                     /locus_tag="O0286_000586"
     CDS             8425..8907
                     /gene="purE"
                     /locus_tag="O0286_000586"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCY0168985.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            8894..10018
                     /gene="purK"
                     /locus_tag="O0286_000587"
     CDS             8894..10018
                     /gene="purK"
                     /locus_tag="O0286_000587"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCY0168986.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            10022..10726
                     /locus_tag="O0286_000588"
     CDS             10022..10726
                     /locus_tag="O0286_000588"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCY0168987.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            10726..10989
                     /gene="purS"
                     /locus_tag="O0286_000589"
     CDS             10726..10989
                     /gene="purS"
                     /locus_tag="O0286_000589"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCY0168988.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            10991..11662
                     /gene="purQ"
                     /locus_tag="O0286_000590"
     CDS             10991..11662
                     /gene="purQ"
                     /locus_tag="O0286_000590"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCY0168989.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            11655..13844
                     /gene="purL"
                     /locus_tag="O0286_000591"
     CDS             11655..13844
                     /gene="purL"
                     /locus_tag="O0286_000591"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCY0168990.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            13823..15307
                     /locus_tag="O0286_000592"
     CDS             13823..15307
                     /locus_tag="O0286_000592"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCY0168991.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            15300..16328
                     /locus_tag="O0286_000593"
     CDS             15300..16328
                     /locus_tag="O0286_000593"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCY0168992.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            16331..16897
                     /gene="purN"
                     /locus_tag="O0286_000594"
     CDS             16331..16897
                     /gene="purN"
                     /locus_tag="O0286_000594"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCY0168993.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            16912..18390
                     /gene="purH"
                     /locus_tag="O0286_000595"
     CDS             16912..18390
                     /gene="purH"
                     /locus_tag="O0286_000595"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCY0168994.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            18412..19659
                     /locus_tag="O0286_000596"
     CDS             18412..19659
                     /locus_tag="O0286_000596"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCY0168995.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(19924..20730)
                     /locus_tag="O0286_000597"
     CDS             complement(19924..20730)
                     /locus_tag="O0286_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0168996.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(20723..22123)
                     /locus_tag="O0286_000598"
     CDS             complement(20723..22123)
                     /locus_tag="O0286_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0168997.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(22138..22713)
                     /locus_tag="O0286_000599"
     CDS             complement(22138..22713)
                     /locus_tag="O0286_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0168998.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(22790..22890)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23297..23431)
                     /gene="graF"
                     /locus_tag="O0286_000600"
     CDS             complement(23297..23431)
                     /gene="graF"
                     /locus_tag="O0286_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCY0168999.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            23583..24878
                     /locus_tag="O0286_000601"
     CDS             23583..24878
                     /locus_tag="O0286_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169000.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGDFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            25303..26475
                     /locus_tag="O0286_000602"
     CDS             25303..26475
                     /locus_tag="O0286_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCY0169001.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            26529..27071
                     /locus_tag="O0286_000603"
     CDS             26529..27071
                     /locus_tag="O0286_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCY0169002.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            27225..27491
                     /locus_tag="O0286_000604"
     CDS             27225..27491
                     /locus_tag="O0286_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCY0169003.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            27494..29212
                     /gene="ptsP"
                     /locus_tag="O0286_000605"
     CDS             27494..29212
                     /gene="ptsP"
                     /locus_tag="O0286_000605"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCY0169004.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
BASE COUNT        10279 a         4314 c         5384 g         9249 t
ORIGIN      
        1 atgcgtgtgt actattattt atcattattt ctatagcgtt aatttttcat cgattacaga
       61 cgaagacaca ttctatagac ccaatacata aggaaacaaa attatcagac aatgaaaaat
      121 atttagtgga tcgtaataag gaaaaggttg cgccgtctaa actaaaagag gtatataata
      181 gcaaggatcc taaatataag aaaattgaca agtatttaca aagttcatta tttaacggtt
      241 cagtagctat atatgaaaat ggcaaattga aaatgagtaa aggttatgga tatcaagatt
      301 ttgaaaaagg tattaaaaac acaccgaata cgatgttttt aataggttca gctcaaaaat
      361 tttcaacagg gttactgtta aaacagttag aagaagaaca taaaataaat atcaatgatc
      421 cagtaagtaa ataccttcca tggtttaaaa catctaagcc tatcccattg aaagatttaa
      481 tgttgcatca aagtggatta tataaatata aatcctcaaa agattataaa aatttagatc
      541 aagcagttaa agcgattcaa aaacgtggta ttgatcctaa gaaatacaaa aagcatatgt
      601 ataacgatgg gaattattta gtacttgcga aagtaattga agaagttaca ggtaaatctt
      661 atgctgagaa ttattataca aaaataggag atcctttaaa acttcagcac acagcatttt
      721 atgatgaaca accttttaaa aaatatctag caaaaggtta tgcttataat agtacaggac
      781 tttcattctt aagacctaat attttggacc aatactatgg tgcaggtaat ttatatatga
      841 caccaacaga tatgggtaaa ttaattactc aaatacaaca atataaatta ttcagtccta
      901 aaataaccaa tccattatta catgagtttg gtacgaaaaa gtatccagat gaatatcgat
      961 atggtttcta tgctaagcca acattgaata gacttaacgg gggattcttt ggacaagtct
     1021 ttactgttta ctataatgat aagtatgtag ttgtacttgc attaaatgta aaaggaaaca
     1081 atgaagttcg aatcaaacat atttataatg atattttaaa acaaaataaa ccttacaata
     1141 cgaagggtgt tattgttcaa taattaatat agaagatata acatgtatat ggcattaagg
     1201 catcgacctt atctgaccag tatacgagtt atatcttctt ttttatagtg gtaaaaagtt
     1261 taaagtataa ggttgaagaa ggatgagttt aaaaatatgt gttaactgat aaaaggggaa
     1321 atcatttggt gagttggcat cagactaaaa tgaatgaaga cgaatacgtt ggtccatgcg
     1381 tggtgtgaat gttttctaaa tctaactgga aatgataatt taataatgta cacttttgat
     1441 tgtttaaaca tgtacaattt ttaaaaacct agggataatt ttattgacgt gaacaacatt
     1501 gagattatct ttagacataa atatggattt tgaagttact atatttatta gttgtggttg
     1561 ttaatgtatt aattgaattg attataatga ttgtcttata agatatagaa ggggacttaa
     1621 ccatttaaaa attgaaggaa aataaaaacc agctaacaaa agtgttagct ggttaaactt
     1681 tacattatta aaatgttgtt tataagtgtg aagagtgacc gccttgcata acccaatatg
     1741 ttccgacaac gaaacaaagt gtaattacaa gagcaaagat aactttgaat gtttgtaaac
     1801 gtccatcttt accttcagtt aaatgcatga acattaataa ttgaagtcct gcttggacga
     1861 atgcaaagcc aaagataatt gtcaacttcg cgtggaatgt taatgacgtg tatagtgtta
     1921 cgtaaactgc taaaagcgtt aatacgatag atgcgataaa tcctacagta tgtttcatta
     1981 ttgtactcat ccgctataca ccatccctat catatatacg gcagtaaaga tgaaaaccca
     2041 aacaacatct aagaagtgcc agtataaact tactataaat aattttggcg cattatattt
     2101 gtctaatccg cgtcgttgga tttggattaa taaacaaatg gcccaaacga tacctagcga
     2161 tacgtgacaa ccatgcgttc ctaataggat aaagaaacta gaccagtaag aaccaattgt
     2221 tgggttaacg ccttctgatg catagtgtgc gaattcataa atttcgaatc caacaaagac
     2281 taaacctaaa agtaacgtaa tgatcatcca aaacatcatt aacttttgtt tttcttggcg
     2341 catgtagtaa atagcaatac cacatgtgta agaactgaat aataatgcaa acgtcattat
     2401 taaaacaaga ggcaattcaa ataactcagt agtcatttta cctgcataat cgccaccatg
     2461 ttgcaaagtt aatagtgtcg caaatagggt accgaataac gcaaattcgg ctgtaatgaa
     2521 aatccaaaag ccaagcttat ttaattcgcc ttcatgtgtg cgtgaatcaa tagtgtttgt
     2581 atcatgactc atgacttaca gcctcccttt ctttaatacg cgcttctctt aatctagctt
     2641 cagtttctgc aacttcagca gcagggatat gatatccgtg atcgatttgg aaactgcgat
     2701 aaatcatagt accaaaaata ccgaataaac aaattaatgc tggaattaca gtttcgaaaa
     2761 ttaagaagaa accgccgata gtcataaaga taccaatcca gaatccaaca ggagtattgt
     2821 ttggcatatg aatgtctttg taattatggt tgtctaagta atgacgacca tgttctttca
     2881 tatcaacaaa tgtgtcgtag tcattccaat ctggtgtaat ggcaaagttg tatttaggtg
     2941 gaattgctga tgcagtagtc cactctagag tacgaccaag gccatcccag ttatctccag
     3001 ttgcttcacg tggagatttg aagtgactgt atacgatact aacaacaagg aataagaatc
     3061 cgattgccat taataatgca ccgatagttg aaattaagtt taataagaac caaccatctg
     3121 atggcatata agtgtataaa cgacgtggca taccatctaa tccaagaatg aattgtggta
     3181 agaaacaaac gttaaatccg atcatgaaga accagaagca ccatttgttt aatgtttcgt
     3241 ttaatttgta acccatcatc tttggatacc agaagattaa accagctaag caggcaaata
     3301 caacaccagt aaccaatgta tagtggaagt gagctactaa gaagtacgta ttgtgatatt
     3361 gatagtcagc tgatgccatt gctaacatta cacccgtaac accacctaaa aggaagttag
     3421 ggataaatgc taatgagaat agcattggtg actcaaatgt aatacgtcct ttatataatg
     3481 ttaataacca gttaaacaat ttcacaccag ttggaatacc aatcagcatt gttgaaattg
     3541 agaagaatga gttgattaac gcaccattac ccattgtgaa gaaatggtga acccaaacta
     3601 agaaactaag gaacgcgatg ccggcagttg cccataccat actttgatgt ccgaataaac
     3661 gcttacgagc gaatgtcggg ataatttctg agtaaatacc aaatgctgga aggataacga
     3721 tataaacttc agggtgcccc catacccaga agaagttagc ccaaagcatt ggcataccgc
     3781 catgtgcaac tgtgaagaat gctgtgtcaa atattctatc agttgtcatt aatgctaacg
     3841 ctactgttaa aggagggaaa gcaagaataa caattaatgt agtaataaat gttgttactg
     3901 taaacattgg catttgcata aacttcatag ttggtgtttt acatcttaaa attgttacaa
     3961 agaagttgat acctgtagct aaggtaccaa gccctgaaat ttgtatagct attaagtaat
     4021 agttaacacc cggaccagga ctgaattcac ctgctagtgg cgcatagttt gtccaaccag
     4081 ctgctggtga accaccaata ataaatgaca ggttgaataa aatcatacct gcaaagaata
     4141 gccagaaact tacgttgttt aatactggga atgcaacatc acgtgctcca atttgtaatg
     4201 gaacaacgat attccataaa ccaaagataa atggcattgc catgaagata atcatgatta
     4261 caccatgtgt actaaaaatt tcgttatagt ggttagattc taaaaatttg ttatcaggta
     4321 ctgttaattg cgcacgaata agtaacgcat caataccacc acggacgaac attaatacgg
     4381 cacagattaa atacataata ccgattttct tatggtctac agatgtgaac cattctttgt
     4441 aaagatattt ccataattta aagtaagtaa ttactgcgat taaaccaata actaagaatg
     4501 gggcaccaat ttgtgccatt gtaatcatcc agttaccttt aactagtaat tgatcccatg
     4561 gaaaattcat taatgtccac ctccatgatc atcattgtct tgatcttgcg catcttttga
     4621 aattttcttc atttctttcg catttttcga ttcatctttc ttgaactcat tgttatatgg
     4681 ttcgtcattt ccaagaatca tcaacttcat accatgtcgt ttatagttcg catttgtaat
     4741 ttgagcttta cgagcaggta ttaatggttt gtctgataca tctttaaaca tattttcttc
     4801 actagtgaag tttggatctt ttaattcgaa attgaaacgt ttatatgcat agaagatgta
     4861 ttctggatcg gctgctggat caacaaacgc catatgtgta ccattaaatt ctaaagcttt
     4921 attaggtgtg cttggtaata attgtttatc aaatgtatct tgatctaacg ttttcttacc
     4981 tttaacttct ttcacccatt tgtcgtagtc tttttgacta acggcattta ctttaaatgt
     5041 ttgacgtgag aatccttcac cattgaagtt agagttacga cctctgaacg taccagtttg
     5101 agatgcttct aacgtccaat tcattgtcat gccagtcatg gcatattttt gaccacctaa
     5161 ttgtggaatc cagaaacttg tcattgtatc catagcttga agcttaaata caacaggacg
     5221 atctttaggg attgttaatg tattaacagt ctctatatgt tcatctggat aagcaaagaa
     5281 ccatttgtat cctgcactta ctgcatatac aaccattgga tctttctcac tcttcggtgg
     5341 tttttcgtaa tcgtataaag ttttaactgt aggaatagct aaagcagcaa cgattatgat
     5401 aggtattaca aaccatattg tttcaatgat ggcattatgg tgcatcttac cagattcggc
     5461 attcttatta taactatact tgtaaataaa aatggcgaac atgccaagta caacgaaaca
     5521 aataacaagc atgaagacga ttgaataaag aatcaagaac ttctgactac ttgctactgg
     5581 cccttttgcg ttgaaaattt ctatatttga acaaccacta agtaaaatta gtgtgccaaa
     5641 taatagaagc aaagacttaa attttgacac ttttttgacc tcctaatact acaaatgtag
     5701 ggcttaacat taattttaag ttattacaca atatttacaa gggcttatgg gaaaaaaatt
     5761 aataaaattg tatcaaaaat gttgataaat caaggtgtga cgtgggttca cacatttgtt
     5821 aaaattatgt gtacattttg tgactaatag cgtttttgaa aaaattgctt catatttaaa
     5881 aggaaagttg agaatagctt tcaattaaag gttaattgaa tgaaaataaa aaaagtttga
     5941 aattgtctaa taaattgaaa atgatggcta gttgattgaa aggaagcgca aacattttaa
     6001 taaggaattt gccaaaagta atttatcgtt aaacaatgaa aagtggaaat ttgtttgttc
     6061 aaaatcaaaa cattttttta aaaagagcag ttaagtttta ataatttgat aggtagtcct
     6121 ttggtataca gaaaaaatac aatatttggg ttaaagttgc agatgaagtt agaataacaa
     6181 ataaaatggt agcaaaacat gttcgtttgc taccatttta tttgcagtat agtgatgagg
     6241 gctatttaac ctcaatatac ctagtggaaa ttttaattgc accatctgaa tcagtgacat
     6301 gatatttaat ttgatattta ccagatttag atgtatcaat ttggccatcg actttaattt
     6361 tatcggttaa atctccatct tctttatcaa atgcacttat gccgtttaga aggttataat
     6421 cttgaccttt cttaataacg atatcattag cgcctttaat ttgtggtgta gtattcgtcg
     6481 ttgcatctgc atttaaattt ggtgttacta gtgtagcaga aacaccgagg gctgataatg
     6541 actgtaatag tttattcata atgtaacctc cgaatgataa gtcatttctg agttagcttt
     6601 actatacctt aatttatatt atttacacat catactatag tcctaatctt accctataaa
     6661 gaatgaaatt taaatataaa tttataaata atgaggaaat aattattatt ttgaaaatct
     6721 gccacaattg taaaattaat aatttaggga taatttgaaa tcaaatataa ctttattcga
     6781 cagtttgaac ttaaattttt gtgtatcaaa acaataagaa aagggaggcg aaaatgataa
     6841 agaaccacta atgattaact atgtagtagt actttatcat agtcacagct tctgtgttgg
     6901 ggcccatccc caacttgcac attattgtaa gctgactttt cgtcagcttc tttgttgggg
     6961 ccctgactag aattgaaaac ttgttacaag cttattttca ttcaattaac tactgtctat
     7021 ataaaattgt tgagccaagt acaaagtgaa atgctccctt caaagccttc atttattcaa
     7081 tgtctacttt gaagggagca tttgatcgat taatgtccca gatttttctt gttattcatt
     7141 gcaattgcaa aataatattt aatgtgggtt aaaagacttt attaaaactc agttattagg
     7201 gagtgggaca gaaatgatat tttcgtaaaa tttatttcgt cgtcccaccc cggcaaggtt
     7261 gactagaatt gaaaaaagct tgttacaagc gaattttcgt tcagtcaact actgccatta
     7321 taactttgta gagcatagaa cattgattta tgtctcaggc tctttacgaa tcaatacctc
     7381 gacgcatttt ttctgcaagc aaagtattat ttaataccat agtaattgtt aatggaccaa
     7441 cgccaccagg aactggtgta atagctccag caatttcttt aaccgcatca taatcaacgt
     7501 caccttttaa tttgccattt tcatctggcg tattgccaac atcgataatt actgctcctt
     7561 ctttgaccac atcttttgtt actaaaccag gcttaccaac tgcactgaca atgacatcag
     7621 catcttttaa atatgatgcc atatcttttg aacgagaatg taagattgtt actgatgcat
     7681 ttttttgaag tagtaactta gaaactggtt gtccgacaat atgacttcgt ccaattacaa
     7741 ctgcattttt accttctaaa tcaatatcag catgttttaa tatttccatg atgccgagcg
     7801 gtgtgcaagg tacaaaagtt tgttcatcga tatataattt ccctatattt attggatgaa
     7861 aaccgtccac atctttttca ggattgattg cttctaatat tttctgttcg ctaacttgtt
     7921 ttggtaatgg tacttgtacc aaaataccac ttacagaatc atcattattt agtctattta
     7981 gttcgtttaa tacttcttct tcagtagctg tttcttccaa atgtacgatt tctgaaatca
     8041 taccaatttt ttcagctgct ttcttttttg atctaacata actttgacta gcgccatcat
     8101 taccaactaa tataacggat aatttaggtg taaaaccctt ttcttttagc gcttcaactt
     8161 gatcttgtaa cccctgtctg tagtctttgg caatttgttt accatctaaa attttagcaa
     8221 ccataaaaac ttctcctcct attgtcaata tcctaacttt gttagtttat aaaactaatt
     8281 tacatagcaa aagacgtaaa ttcaaacaaa aaatcaaaat aaagttcgat ttttgattga
     8341 aaaagcagaa attgcttgtt atgctatatc tataatatac aactaaagta attcacaatt
     8401 caaacttttg aaagggtgta caaagtgaaa gtagcagtca ttatgggcag ttcttccgat
     8461 tggaaaatta tgcaagagag ttgtaacatg ttggattatt ttgaaattcc gtacgaaaaa
     8521 caagtagtat ccgcacatcg tacgccaaaa atgatggttc aatttgcttc tgaagcgaga
     8581 gaaagaggta taaacattat cattgcaggc gctggcggtg cggcacattt accaggtatg
     8641 gttgcatcat taacgacgct accagttatt ggagtgccga ttgaaacaaa aagtttaaag
     8701 ggtatagatt ctttattatc aattgttcaa atgccaggag gtattccggt tgcaacgact
     8761 gcaattggtg cagcaggtgc taaaaacgca ggtatacttg cagcaagaat gttaagtatt
     8821 caaaatcctt ctttagttga aaaactaaat cagtatgaat cttcgttaat tcaaaaagtg
     8881 gaggacatgc aaaatgaact tcaataaatt aaagtttggt gcgactattg gcattattgg
     8941 tggtggtcag cttggaaaga tgatggcaca atcagctcaa aaaatgggtt ataaagtggt
     9001 tgtattggat ccttctgaag attgtccatg tagatacgtt gcacacgaat ttatacaagc
     9061 caagtatgac gatgaaaagg cactcaatca attaggacaa aaatgtgatg tgattactta
     9121 tgaatttgaa aacatttcag cccaacaatt aaaactatta tgtgaaaagt acaatattcc
     9181 gcaaggttac caagctatac agttattaca agatcgctta actgaaaaag aaacattaaa
     9241 aagtgctggt accaaagttg tcccgttcat ttcagtaaaa gaatctacag atattgacaa
     9301 agcaattgaa acattaggat atccttttat tgtaaaaact agatttggtg gctacgatgg
     9361 caaaggtcaa gttttaatta acaacgaaaa agacttacaa gaaggtttta aattaattga
     9421 aactagtgaa tgcgtagctg aaaaatattt gaatatcaag aaagaagtat ctcttactgt
     9481 tacaagagga aacaacaatc aaatcacttt tttcccatta caagaaaatg agcatagaaa
     9541 tcaaatactt ttcaaaacaa ttgttccagc gagaatagat aaaacagctg aggcgaaaga
     9601 gcaagttaat aaaattatcc aatcgattca tttcattgga acatttacag ttgaattttt
     9661 tatagatagt aacaaccaat tgtatgtgaa cgagatagca ccaaggcctc acaattccgg
     9721 acattattca attgaagcat gtgattattc acaatttgat actcatattt tagcagttac
     9781 cggacaatca ttaccaaatt caattgaatt attaaagcca gcagtcatga tgaacttact
     9841 aggtaaagat ttagatttat tggaaaatga atttaatgaa catccagagt ggcacttaca
     9901 tatttatggt aagtctgagc gtaaagatag cagaaaaatg gggcatatga ctgtactaac
     9961 gaatgatgta aaccaaactg aacaagatat gtacgctaaa tttgagggga gtaattaaag
    10021 catgacatta ttatatgaag gaaaagcgaa gcgcattttc tcaacaaatc aagaaaatga
    10081 attaagagtt gaatataaag atgaagttac tgctggaaac ggggctaaga aagacacaat
    10141 ggcaggtaag gggcgattaa ataatcaaat tacttctatt atatttaaat atttacaaga
    10201 aaatggaata gaaagtcact ttattaaaca attatctgaa acagaacaat tagttaagcc
    10261 tgtgaaaata attccattag aagtagttgt tcgtaatatt gctagtggat ctattacaaa
    10321 gcgtttaggt tttgaaaatg gtgaagtttt tagagaacca cttgtagaat ttttctataa
    10381 aaatgatgcg ttaaatgatc cgttgataac ggatgaccat gttaaattgc tcaatatagc
    10441 atcagatgaa gatattgaaa tactaaaatc caaagcatta aagattaata atgtgttgaa
    10501 acaattaatg gatgctatga atttaaaatt agtagatttt aaaatcgaat ttggaaagac
    10561 tgagactggt caaattttgt tagcggatga aatatctcca gatacatgtc gaatttggga
    10621 taaagctacc aatgcaaact ttgataaaga tgtatataga aataacactg gatcactgat
    10681 tgaaacatat caaatatttt taaacaaatt ggaggattta aaataatgaa aacaattgaa
    10741 ctacatatca cattacaacc acaagtatta gatacgcaag gacaaacgct tactcgagct
    10801 gtacatgact taggttatgc acaagtgaat gatattcgtg taggaaaagt attatatatg
    10861 acagtggatg aggttagtga tgaaaaggta cacaacatta ttacaactct aagtgaaaaa
    10921 ttgtttgcaa atacagtgat tgaagaatat agctataaag tgttagatga tgaaaaggag
    10981 aatgcataaa atgaaatttg cggttcttgt ttttccaggt tcgaattgtg atagagacat
    11041 gtttaatgct gctattaaaa gtggtgttga agcggaatat gtagattata gagaaacatc
    11101 actaagtgga tttgatggcg tacttattcc tggtggattt tcattcgggg attacttaag
    11161 atctggggca atggctagtg tagcgccgat tatttcggaa gttaaacgtc ttgcagctga
    11221 aggtaagcca gtattaggtg tttgtaatgg gtttcaaatt ttaactgaaa taggcttatt
    11281 acctggtgca ttattgcata acgattcaca tttatttatt agtagaaatg aagagttaga
    11341 aatagtgaat aatcaaacgg catttacaaa tctttatgaa caaggtgaaa aagttatata
    11401 tcctgtagct cacggtgaag gtcattatta ttgtactgat gaaatatatc aacaattaaa
    11461 agctaacaat caaattattc tgaaatatgt gaataatccg aacggttcat atgatgatat
    11521 tgcaggaatt gttaacgaaa aaggcaatgt atgtggcatg atgccacatc ctgaaagagc
    11581 tttagaaacg ttgttaggta ctgatagtgg tgtgaaatta tttgaagcga tggtaaaaag
    11641 ttggagggaa caacatgtct aaatttatcg aaccaagcgt tgaagaaatt aaacttgaaa
    11701 aagtatatca agatatggga ttaagtgatc aagaatatga aaaagtttgc gatattttag
    11761 gcagacaacc taactttaca gaaacaggta tcttttctgt tatgtggagt gaacattgct
    11821 cttataaaca ttctaaaccg tttttaaagc aatttcctac gtcaggtgac catgtgctta
    11881 tggggcctgg tgaaggtgca ggggtagtcg atataggtga taatcaagcc gtagtattta
    11941 aagtagagtc tcacaatcat ccatcagcaa ttgaaccata tcaaggggct gctacaggcg
    12001 ttggtggaat cattcgtgac attgtctcta ttggggctag acctattaat ttgttaaaca
    12061 gtcttagatt tggagaatta gataataaac aaaaccaaag attacttaaa ggtgttgtaa
    12121 agggtatcgg aggttatggt aactgcattg gtattccaac aactgctggt gaaatcgaat
    12181 ttgatgaacg ttatgatggc aatccacttg ttaatgcaat gtgtgttggt gttatcaatc
    12241 acgacatgat tcaaaaaggc acagcaaaag gtgtaggtaa ttcggtcatt tatgttggtt
    12301 tgaaaactgg tcgagatggt attcatggtg ctacttttgc atctgaagaa ttgacggaag
    12361 aaagcgaaag taaacgacct tctgtacaaa tcggtgatcc atttgtaggt aaaaaattaa
    12421 tggaagcaac acttgaagca attacatttg atgaattagt tggtattcaa gatatgggtg
    12481 ctgctggttt aacatcttca tcgtctgaaa tggcggcaaa aggtggtagt gggttacatt
    12541 tgagattaga acaagtgcca acacgtgagc caggtatttc tccttatgaa atgatgcttt
    12601 cagaaactca agaacgtatg ttactagttg ttgaaaaagg tactgaacaa aaattcttag
    12661 atttatttga taagcacgaa ttggatagtg ctgttatagg tgaagttaca gatacaaatc
    12721 gttttgtttt aacatatgat gacgaagttt atgctgacat tccagttgaa ccactagctg
    12781 atgaagcacc tgtatatatt ttagaaggag aagaaaaaga ttataatact tctaaaaatg
    12841 attatacaca catcgatgtt aaagatactt tctttaaatt acttaagcat ccgactatag
    12901 catctaaaca ctatttatat gatcaatacg accaacaagt tggtgccaat acgataatta
    12961 agccaggact tcaagcatcg gtagtacgtg tggaaggcac aaataaggca attgcttcaa
    13021 caattgatgg tgaagcgcgt tatgtatata acaatccata tgaaggtgga aagatggtag
    13081 tagctgaagc ttatcgaaat ttaattgccg tgggtgcaac accattagca atgacagatt
    13141 gtttaaatta tggttctcct gaaaagaaag aaatctatca acagttgata gattcaacga
    13201 aaggtatggc agaagcatgc gacattctta agacaccagt agtttctggt aatgtatctt
    13261 tatataacga aacgaaaggt acttctattt tcccaacacc agttgttgga atggtaggtt
    13321 tgattgaaaa tgtaaattat ttaaatgatt ttgaacctca agttggagat aaattatatt
    13381 taatcggtga tactaaggac gactttggtg gtagtcaact tgaaaagtta atttatggca
    13441 aagttaatca tgaatttgag tcattagatt tgagttcaga agttgaaaaa ggtgaatcaa
    13501 tcaagaccgc tattcgtgaa ggactattat cacatgttca aacagttggt aaaggtggct
    13561 tactgattac cttagctaaa ctaagtgcgc attacggttt aggattaaaa tcttcaatag
    13621 atataacaaa tgcacaattg tttagtgaga cgcaaggccg atatgttgtt tctgttaaat
    13681 caggtaaaac tttaaatatt gataatgcaa tagaaattgg acttttaaca gatagtgata
    13741 atttcaaggt aacaacacca tatacagaga ttagtgaaaa tgtttcagat attaaacaaa
    13801 tatgggaagg ggcaattgct caatgtttaa ctactcagga ttaaacgaag aatgtggcgt
    13861 gtttggtatt tggaatcatc ctgaagcagc gcaactaaca tatatgggac ttcatagttt
    13921 gcaacatcgt ggtcaagaag gtgcaggtat agttgtttct gatcaaaatg aattaaaagg
    13981 cgagcgagga ttaggcttac taactgaagc gattaaagat gatcaaatgg aacgattaaa
    14041 aggatatcaa catgcaattg gtcacgtccg ttatgctact tcaggtaata aaggtattga
    14101 aaatattcaa ccgtttctgt atcactttta tgatatgagt gtaggtattt gtcataatgg
    14161 taacctcatt aatgctaaat cattgcgtca gaatttagaa aaacaaggtg ctatcttcca
    14221 ttcgtcttct gatactgaag tcattatgca tttgatacgt cgaagtaaag ctcctacttt
    14281 tgaggaagcg ttgaaagaaa gtttgcgaaa agttaaaggc ggttttacat ttgcgatttt
    14341 aactaaagat gcgttatatg gcgcagtaga tccaaatgct atcagaccac ttgttgtagg
    14401 taaaatgaaa gatgggacat acatccttgc aagtgaaaca tgtgcaatag atgtgttagg
    14461 tgcagaattt gttcaagata ttcatgcagg tgaatatgtc gtgattaacg ataaaggtat
    14521 tacagttaaa tcttatacac atcatacgac aactgcaatt tctgcgatgg aatatattta
    14581 ttttgctaga ccagactcaa caatagctgg taaaaatgtc catgcagtac gtaaagcttc
    14641 tggtaaaaaa ttagcccaag aaagccctgt aaatgctgat atggtcatcg gtgtacccaa
    14701 ttcatcgcta tcagctgcga gtggttatgc tgaagaaata ggtttgccat atgaaatggg
    14761 actagttaaa aatcaatatg ttgcaagaac atttattcaa ccaactcaag aattacgtga
    14821 gcaaggtgtg agagtgaagt tatctgcggt aaaagatata gtagatggga aaaatatcat
    14881 tcttgttgat gattccattg ttcgcggtac gacaattcga cgcattgtga aaatgttaaa
    14941 agattctggt gcaaataaag tgcatgtgcg tatagcatca ccggaattta tgtttccaag
    15001 tttttacgga atcgatgttt caactacggc agaattaatt tctgcaagca aatcacctga
    15061 agaaattaaa gattatattg gcgctgattc attagcatat ctatctgtag atgggttaat
    15121 tgaatcaatt ggtttagatt atgacgcgcc atatagtggc ttatgtgtag aaagtttcac
    15181 tggagattat cctgcagggt tatatgatta tgaagcaaat tataaagcgc atttaagtca
    15241 tcgacaaaag caatatattt ctaaaaacaa acactttttt gatagcgagg gaaatttaaa
    15301 tgtctaaagc atatgaacaa tctggtgtaa atattcatgc tggttatgaa gctgtagaaa
    15361 gaatgtctag tcatgttaaa cgtacgatgc gtaaagaagt tatcggtggt ttaggtggat
    15421 tcggtgctac atttgattta tcacaattaa atatgacagc gccagtttta gtttctggaa
    15481 cagacggagt aggtacgaaa ttaaaactag ctatcgacta tggtaaacat gattcgatag
    15541 gtatcgatgc agtcgcaatg tgtgttaatg atattttaac gacaggtgca gaaccattat
    15601 attttttaga ttacattgct acaaacaaag ttgttcctga agttattgaa caaattgtta
    15661 aaggtattag tgatgcatgt gttgaaacga atactgcact tatcggtgga gagactgctg
    15721 aaatgggtga aatgtatcac gaaggcgaat atgatgtagc tggatttgct gttggagcag
    15781 ttgaaaagga tgactatgta gatggttcag aagtgaaaga gggacaagtt gttatagggc
    15841 ttgcgtcaag tggcattcat tcaaatggat acagtttagt gcgcaaatta attaatgaat
    15901 caggcattga tttggcatca aacttcgata atcgtccatt tatcgatgtc tttttagaac
    15961 caactaaatt atatgtcaaa cctgtacttg ctttgaaaaa agaagtttct attaaggcaa
    16021 tgaatcatat tactggtgga ggtttttatg aaaatattcc acgtgcattg ccagccggat
    16081 atgctgctag aattgatact acatcatttc caacaccaaa aatatttgat tggttacaac
    16141 aacaaggcaa tatagacaca aatgaaatgt ataacatttt taatatgggt attggctata
    16201 cggttatcgt tgatgaaaaa gatgtatcac gcgctttgaa gattttagca gaacaaaatg
    16261 tggaagccta tcaaattggt catattgtga aaaatgagtc aactgcaatt gaattgttgg
    16321 gggtataaca atggttaaaa ttgcgatttt tgcatcaggt tcaggaagta actttgaaaa
    16381 tatagttgag catgttgaat caggaaaact tgaaaatatt gaagttacgg cgctatatac
    16441 ggatcatcaa aatgcgtttt gtatagatag agcaaaaaag cacgatattc ctgtttatat
    16501 taatgaacca aaacaatttg attcaaaagc agcgtatgaa caacatttag taacattatt
    16561 aaatgaagat aaggtagagt ggattattct agctggctac atgcgtctaa ttggtccaga
    16621 cttattagct tcatttgaag gtaaaatatt gaatatacat ccatctctat tgccgaaata
    16681 taaggggatt gacgcaatag gccaagcata tcatagtggc gatactatta ctggttcgac
    16741 agtacattat gttgatagtg gtatggatac gggagaaatt attgaacaga gaaaatgtga
    16801 tattagaccg gacgattcaa aagaacaatt agaagagaaa gtaaaaaaat tggaatatga
    16861 gttatatcca agtgttattg ctaaaattgt aaaataagga gtatgtatgt gatgaagaaa
    16921 gctattttga gcgtatcaaa taaaacaggt attgtagagt ttgctaaagc gttaacgcaa
    16981 ttaaattatg aattatattc aacaggtggt actaaacgta tattagatga agcaaatgta
    17041 ccagttcgtt ctgtttcaga cttaacacat tttccagaaa taatggatgg ccgtgttaaa
    17101 acattacatc cggctgttca tggtggcatt ttagctgatc gaaataaacc gcagcattta
    17161 aatgaattat cagaacaaca tatagattta attgatatgg tagtagttaa tttatatcca
    17221 ttccaacaaa ctgttgcaaa ccctgatgtg acgatggacg aagcaattga aaatattgat
    17281 attggtggtc caacaatgtt acgtgctgca gctaaaaact ataaacatgt aacaacaatt
    17341 gtacatccgg cagattatca agaagtattg acgcgattaa gaaacgattc gttagatgag
    17401 tcatatagac aatcattaat gattaaagtt tttgagcata ctgcagaata tgatgaagcg
    17461 attgtacgtt tctttaaagg ggataaagaa actttaagat atggagaaaa tccacaacaa
    17521 tcagcgtatt ttgtgagaac ttcgaatgct aagcacacga ttgcaggcgc taaacaatta
    17581 catgggaaac aattaagcta taacaatatt aaagatgcag atgctacact agctttagtt
    17641 aaaaagtttg atacacctgc tacagttgcg gttaaacaca tgaatccatg tggtgttggt
    17701 atcggtgaca cgatagaaca agcatttcaa catgcgtatg aagcggatag tcaatcaata
    17761 tttggtggaa ttgttgcatt aaaccgagct gtaacacctg agttagcaga gcaattgcat
    17821 agtatctttt tggaagtcat tattgcacca aaatttacag atgaagcatt agatatttta
    17881 aaacaaaaga agaatgtaag attattagaa attgatatga ctatagacag taacgaagaa
    17941 gagtttgttt cagtatctgg cggatattta gttcaagata aagacaatta tgtcgtgcca
    18001 aaagaagaaa tgaaagttgt tacagaagta gcacctacag atgaacaatg ggaagcaatg
    18061 ttattaggat ggaaagttgt accatcagta aaaagtaatg caattatttt aagtaataat
    18121 aaacaaactg taggtatagg tgctggacaa atgaatcgtg tcggtgctgc taaaattgcg
    18181 ttagagagag ctattgaaat caatgatcat gtagcgttag tatctgatgg atttttccct
    18241 atgggagata cagttgaact tgcagcacaa catggtataa aggcaattat ccaaccgagt
    18301 ggttcgatta aagatcaaga ttcaattgat atggctaata aacatggtat tgcaatggta
    18361 gtcacaggca ctcgacattt taaacactaa ttttaaaggg gcgtaacata aatgaatgta
    18421 ttagtaattg gtgctggtgg acgagaacat gcacttgcat ataaacttaa tcaatcgaat
    18481 ctagttaaac aagtgtttgt cattccaggt aatgaggcaa tgacacctat agctgaagta
    18541 cacactgaaa tttcagaatc tgatcatcaa gcgatactag attttgctaa acggcaaaat
    18601 gttgattggg tagttatagg tccagaacag ccgctaattg atggattagc agacatttta
    18661 cgagcgaatg gtttcaaagt gtttggtcca aataagcaag cagctcaaat cgaaggctca
    18721 aaattatttg ctaaaaagat aatggaaaaa tataatattc caactgctga ttataaagaa
    18781 gttgagcgaa aaaaggatgc tttaacatat attgaaaact gtgaattgcc cgttgttgtc
    18841 aagaaagatg ggttagctgc tgggaaaggc gttattattg cagatactat tgaagcagcc
    18901 agaagtgcta ttgagattat gtatggtgat gaagaagaag gtactgttgt atttgaaacg
    18961 tttttagaag gtgaagagtt ctcgctaatg acatttgtta atggtgattt agcagtacct
    19021 ttcgactgta ttgcacaaga tcataaacgc gcatttgatc atgatgaagg accaaatact
    19081 ggtggtatgg gggcttattg tccagtacca catattagtg acgatgtttt aaaacttaca
    19141 aatgaaacaa ttgcacaacc cattgcaaag gcaatgctta atgaaggtta tcaattcttc
    19201 ggtgtattat acattggtgc tattttaact aaagatggtc caaaagtaat agaatttaat
    19261 gcccgttttg gtgatcctga agctcaagta ttattaagtc gcatggaaag tgatttaatg
    19321 cagcatatta ttgatttaga tgaaggaaaa cgtactgaat tcaaatggaa aaatgaatct
    19381 attgtagggg tcatgttggc atcaaaagga tatcctgatg catatgaaaa agggcataaa
    19441 gtaagtggct ttgatttaaa tgaaaactat tttgttagtg gattaaagaa gcaaggtgat
    19501 acctttgtta cttcaggtgg tagagttata cttgccatcg gaaaaggtga caatgtacaa
    19561 gatgcacagc gagacgcata caaaaaagta tcacaaatac aaagtgacca tttattctat
    19621 cgtcatgaca ttgcgaataa agcactacaa cttaaataag taaatttaaa atactaagat
    19681 tagctatgaa cgaatctata acgatagatt ttttcatagc ttttttagtt gtagagtcta
    19741 ggacattgat ttctgtacca aatttgtgat tatgcatatg taatacaaaa gaggcgccac
    19801 aacatgtttg gatgaacaaa ataacatgtt tgtggcacct cttttgttta gtatggaata
    19861 aatggttttc tttttctata caatgaattt ctaatttagt atctatacaa ttatggataa
    19921 aatttaacct acacgaccaa gacgaacatc atctatgccc gtgatgggta aggtgattga
    19981 acaataatat gccatagtaa taatggcaat taaaactata ataaagatta tatctttata
    20041 tgagaaaggt acgttgtaat agtaagtacg aggaccatct ctaaatcctt tcgactccat
    20101 cgcaactgat aattgatgtg cctttctaat attttggctt aatagaggta taattaaatg
    20161 cttaaatcgc tttaaccctc tataatttgc cgcgtctatc atctgatagc gcatttttaa
    20221 agatctgcga agctgtatta aagaactaat cattaaaggt atcatacgaa tggcagccat
    20281 gaatgcataa gcaacttttg atttaacctt taaatgttgc attaaactat aaaatatcat
    20341 gacaacctga gatgtaagtg cgattaagat accgaaaaat gaaatagcaa tggttcttaa
    20401 tgatacatgt aaaccacgaa ctaaactttc tgttgtaata tggataaatc cgaatttcaa
    20461 aattgtatgg ctaccattcc cgtataaaat catgaacagg gaagagagta atgcaaagcc
    20521 aatacttata gttataaaaa ttgctgtaat tttaaactga gtaccattaa acatcaataa
    20581 gaaaactaac attaagatag tgatataaag cataaaatcg aaattatgca caaatataat
    20641 aaagaaaaat agtataattc caagaaatag tttcgttata atgttgacat catcaacaaa
    20701 tgattgccga actttccatt gctcatacat tcgtatcacc atcacaatct agtaacgcac
    20761 catctgaaat tttaagtctt cttgatggat aacgttcaat tatttcatcg tcatgtgtaa
    20821 ccatgacaat actttgtccc aaattaattc gcttttggaa aagtttgatc aactggaatg
    20881 tattatggct atcaagtcca aatgtcggtt catctaaaaa gataatatca gctttagaac
    20941 ttagtgcggt agctacgcta aggcgtcgtt tttgaccaat agacaactca taaggatgtt
    21001 gatcttttac attttgtaaa tctaaaagtt ttaaaagttg tatcgtttca tcatcacttt
    21061 gatctttaga aaggtgatta aaatgaatgt taatttcatc ataaaccgaa tttgttataa
    21121 attgtaattc tgggttttga taaactaggt acatgtgttt tgctgcatgt ttaatttttg
    21181 ttaaacgctg attttcaaaa taaacatcac cttgatattt aatcaattgc ataattgatt
    21241 caagcaaggt tgttttacca ctaccatttg cccctgtaat tgtaatccac tcacctagac
    21301 caatttctaa atctgagaat gagagcaatg ttgatttacc gcgaataata cgtccatttt
    21361 taaattgtaa taagtgtgag tttgttgttg gaaagtcaac acgacttggt gcgaattccc
    21421 atgcacgtgg atgccacaca ccatattcac tgagtaaatg aacatacttc tgtaatatga
    21481 tttcaggaca ttcatcggca atgatatttc cgttataatc catcaaaatg acgcggtcga
    21541 catgattcca gatgtgttta actttatgtt caacgattac aaccgtttga tcttcccaaa
    21601 gttcaattag tttagtccat aaatcttctg ttgcttgaac atctaacatt gctgtcggtt
    21661 catctaaaaa caatgttttt gattgttgaa gaatggtttc aacaattgcc aatttctgtt
    21721 tcatcccgcc acttaaatct ttgatatacg tttcaggggt aacatttaaa ttgaccatat
    21781 ttaaagcatt gataattaac gcatccatgt cttcacgtgg taattgtcta ttttctaaaa
    21841 cgaatgcaag ttcttcgtat acttttggca tacaaaactg gctatcaggg tcttggaaaa
    21901 taacgccact taatgggtca acgattagtt catcatattt cataggtaat tcaattaaat
    21961 taggaacaat accacttaat acattcagaa gtgtactttt accgcaacca gaaggaccga
    22021 gtaaaagtac tttttctttg tcttgaatag tgatatttaa atgatcgaaa attttacgtt
    22081 gaccacttgg atattttaat cgtaaatcac ttacttttaa cactctgaat gctcctctta
    22141 taagttgtcg taatcttctt tagcagctgg tctaaataat tttgttacgc ctgtcttatc
    22201 taaagctttt actaaaaggt aagataggac gccggcgact actgcaccac taattaatct
    22261 aaatacgatg aataatgtta agttccaacc tgcaacttca tttaaataac catagaaata
    22321 atctatcggg aaagccgcga ttgctgtaca aaaacctgct aacatagcta ccataactga
    22381 acgtgattga tatttaaaaa ttgcaaagac aagttcacac gctaaacctt gtataaaagc
    22441 gtaaacgatt gtcggaatat cgaaacgacc cataatgata gtttcgccgg cacctgcagc
    22501 aaattcagcc agtaaagcaa tacctggttt tggaataatt agatagcaga caatcgctgc
    22561 catgaaccaa accccgtttg ttaattgttc gaggtgaagg cctgtagctt gcacaccatt
    22621 gtaaacaaac caccataaat tgtaaataac tgcgaatact actgaaataa gtacggttac
    22681 tagtatttca gatagcttta aaccttttga catttttaca tcctcctaat aaaaaaacgc
    22741 acaaccatcc ataggaaagt tatgcgttca caatatatat tagtaaaaca tatgtatagt
    22801 aacactttcc tacgctagtt caagctagat caggttcaaa gggtttgagg gcaagcctca
    22861 tctcagtata aaacacccct agtgtgtgcg atttatttaa ttaattatac tgtaagacgt
    22921 ttgtaaactt atgtcaatag gttgtcttca tgaaatttcg tttaattcga tttaaaattt
    22981 ataatattag cattggattt aaattgaaga tgtagtagga atgttagtaa ttaaagatat
    23041 aaaaatatgt gacatgtaat aatattgagc tgataaatga agagggatac ttatcaatca
    23101 tacctcttta acaacagtga agaacccgtg cataatggct tacgaattat agtttataag
    23161 gaagaagagg gatacatgcg ccgagcacat gcataaaagc ccctaacaac taaaagttgt
    23221 aaggaaggag agggatacat gcgccgagca catgcataaa atcccctaac aactaaaagt
    23281 tgtaagggga tttaaattaa tttagtgtat cttggatatc ttgttttgtt tgattaatat
    23341 cttctgtttt ttcttctttt ttatctttta atttttcttc aacttcttta gctttttctg
    23401 ctgctttttt attttgattt tcattagaca tgattaattc ctcccaaatt ggataattat
    23461 ttatatataa atcttacccg gttgtacttt cgttaaactt ttctaagtct atagcactat
    23521 ttattcattt atctaaagac aacaacatta gattaatata taatgatttt gaggtgaaca
    23581 taatgtcttt tcttaggaaa cacgccgaaa ttatttttag ctatttaatc ggtatcgttt
    23641 cactcttcac tggtctcatt attttaatta acttgccatt aattaaacaa ttaaatggtg
    23701 gtaaaaaagt tgatacacat gttcataatg tgtgggaatt tctgaatgca tttttcagtg
    23761 aaattattaa agtaatgagt cgatttatag gtgatttccc tatagttagt gcaattgtga
    23821 taattatatt cggtatttta gttatgttga ttggtcatac attacttaga actattaagt
    23881 atgactatga tatttctatc tttttcttag ttatcggtat catgtacttt attattactc
    23941 ttatattaat gactcaagtt tatggattct ttgcagtgat tttcattatt ccatttacaa
    24001 ttcatatagg atatatcgtc tataaagatg aattgaatca ggaaaatgta aaaaatcatt
    24061 tcatgtggat aattgtgagt tatggtataa gttacttaat tacacaaatt gcattgtatg
    24121 gcagaattga tgctaatgaa atagagtcaa ttgatatctt aagtgtcaat gctttcttta
    24181 taattatgtg gttacttggt caaatggcta tttggaattt cttgttcttg cgccgagctt
    24241 tacctttaac aaagcaagaa ttaggtgaag aggagccaga attatcaaga acaagtaaag
    24301 ggaatgtcac gaatcaaact aaaattcact tgaaacaact ccaagataag actacagaat
    24361 atgcacgtaa gacaagaaga agtgtcgatt tagataaaat tagagctaaa agagataaat
    24421 tcaaaaagaa agttaatgat attatcgata ttcaagaaga cgatattcct gattggatga
    24481 gaaaaccgaa atgggttaaa ccaatgtatg tcgaactatt ttgtggtgtc gtcatctttt
    24541 tattcacatt tttagaattt aataatcgta atgcattatt tgtatctggt gattggaaat
    24601 tatcacagac acaatatgtt attgaatggg ttacattatt aattctgtta ttcattatta
    24661 tcgcatatat cgctacaacg ttaactttcc acttgaaagg taagttttat tatttacaat
    24721 tatttatggg gagcatttta ttctttaaat tgttaacgga atttataaat ataatgattc
    24781 atggactatt actttcagtg ttcattacgc caacattact attaatgtta ttggcaatca
    24841 tcatttctta ttcgttacaa ttacgagagc gaccataatt aaaagcatta taaaagtact
    24901 atctattaaa cattttgatg tgtacgctat aagttagata tatctctaac ttacttagat
    24961 acaggtcaat gaagtttatg gatagtactt tttttgtaac tagatttgat tgattcaggt
    25021 gatgtgaatt aagtattgaa aattgtatac aaagttttag tgcaaataaa atagttgaaa
    25081 agttatccat ttgtaaaatc aagaaaacta gtaaatagtt gaagcgactt atggaatttg
    25141 cgaaacgata tatagtattt cctttgtaga aatttaacat atatcattcg aattactaat
    25201 ttgttaaaat caacagtaag attagaagta gatgatattg aaatttggca aacaatttaa
    25261 tctatataaa actacaacga aacacagaaa ggaagttgtc agatgaaaat agcaactctg
    25321 aacaaaggca aagaaacaaa atattttaat ggatatcctt taattgaaga agaggatatc
    25381 tattcacaag atcatttaaa agaaggagat atttttcaaa ttgtgactga taaatcacaa
    25441 tatgtcgcaa cggcttatgt tggtcgccaa cataaaggat taggttgggt tctaacatac
    25501 gataaagctc aacaaatcaa cacagctttc tttgtaaaat tgtttaatac tgcattagca
    25561 gaacgtgatt attattttaa tatagatgga acaaatgctt ttagattatt taatgctgaa
    25621 ggtgatggtg ttgggggatt aacaatcgac aattacgatg gtcatttgtt gattcaatgg
    25681 tactcaaaag gtatttataa atttaaatat gccattcttg aagcggttag aaaagtattt
    25741 gattataaat ctatttacga aaaagtaaga tttaaagaca gcgaatatag tggtggtttt
    25801 gttgaaggag atgcacctga gtttccaatt gttatcgaag aaaacttcac attttataat
    25861 gtagaccttg aagatggttt gatgacaggt atctttttag atcaaaaaga agtgcgcaag
    25921 aaattaaggg atcaatatgc caaagaacgc catgttttaa acttatttag ttatacaggt
    25981 gctttttctg caatagcagc aagtgaggca tcttcaacaa caagtgtaga tttggctaat
    26041 cgttctcgta gtttaactga agaaaatttt ggattaaatg ctattgatcc taaatcccaa
    26101 tatatttatg tcatggacac ttttgatttc tataaatatg ctgcacgaca tggacatagt
    26161 tatgacacga tcgtgattga tccacctagc tttgcgcgta acaaaaaacg tacattttca
    26221 gtgcaaaaag attatgacaa attaattaat ggcgccttaa atatcttatc atctgaagga
    26281 acattattgt tatgtacaaa cgcaagtgta tatccattaa agcaatttaa aaatactatt
    26341 aaaaagacgc ttgaagagag tggcgttgat tatgaattaa ctgaagttat gggattacca
    26401 aaagatttta aaacgcatcc acattataag ccatctaaat atttaaaagc tgtttttgta
    26461 aatattagac attaatttga taaaaagggg tatagtaact tattgagaaa aagaagggtg
    26521 ataatattat gggattcaaa aacaatttaa catcaaattt aacaaataaa atcggtaatt
    26581 cagtctttaa aatagaaaat gttgacggaa aaggtgcaat gccaacgacg attcaagaat
    26641 tgagagaaag acgacaacgt gctgaagcaa ttgtaaagag aaagtcttta atgtcatcaa
    26701 caatgagcgt tgttccaatt ccgggtttag attttggtgt tgatttaaaa ttaatgaaag
    26761 atattatcga agatgttaat aaaatttatg gtttagatca taagcaagtt aatagccttg
    26821 gggatgatgt gaaagaaaga attatgtctg cagcagcaat tcaaggtagt caatttattg
    26881 gtaaaagaat ttcaaatgca tttttaaaaa ttgtaattag agatgtagct aaacgtactg
    26941 ctgcaaaaca aacaaaatgg tttcctgttg taggacaagc tgtgtctgca tctattagtt
    27001 actattttat gaataaaatt ggaaaagatc acattcaaaa atgcgaaaat gttattaaaa
    27061 atgtcatgta ggtgctataa tagttttgca atttgcaaat tttactgaaa ccggttttaa
    27121 acgaattgaa tttaaagcat ggttttggta aagttaatgt ataaaactaa gttagtattg
    27181 taataatatt gaagattcta actatacgaa ggagaaatgt aattatggaa caaaattcat
    27241 atgtaatcat cgacgagact ggtattcacg ctagaccagc aacaatgtta gtacaaacag
    27301 cttcaaaatt cgattctgat attcaattag aatataacgg taagaaagta aacttaaaat
    27361 caatcatggg tgttatgagc cttggtgttg gtaaagatgc tgaaattaca atttatgctg
    27421 acggtagtga tgaatctgac gccattcaag caatcagtga cgtcttatca aaagaaggat
    27481 tgactaaata atcatgtcta aattaattaa aggtattgcc gcatctgatg gtgtcgcaat
    27541 tgctaaagct tatttattag ttgagccaga cttaacattc gacaaaaatg aaaaagtcac
    27601 tgatgttgaa ggagaagttg caaagttcaa tagcgctatc gaagcttcta aagttgagtt
    27661 aactaaaatt agaaataatg cagaggttca actaggtgct gataaagctg ctatctttga
    27721 tgcacattta ttagttttag atgaccctga attaattcaa ccaatccaag ataagattaa
    27781 aaatgaaaac gctaatgctg ctacagcatt aacggatgta acaacacaat ttgttacaat
    27841 ttttgaatct atggataacg aatacatgaa agaacgtgcg gctgatattc gcgacgtttc
    27901 taaacgtgtg ttatcacata ttttaggtgt agaattaccg aatccgagta tgattgatga
    27961 aagcgttgtt attgtaggga atgacttaac gccatctgat actgctcaat taaataaaga
    28021 attcgtacaa ggttttgcta caaacattgg cggaagaaca agtcactctg caattatgag
    28081 tcgttcttta gaaattccag caattgttgg tacaaaatca attactcaag aagttaaaca
    28141 aggcgacatg attatcgtag atggattaaa tggtgatgta atcgttaatc caactgaaga
    28201 tgagttaatc gcttatcaag ataaacgtga gcgttatttt gctgacaaga aagaattaca
    28261 aaaactacgt gatgctgata ctgttacagt tgatggtgtt cacgcagagc ttgctgcaaa
    28321 tattggtaca cctaatgatt tgccaggtgt tattgaaaat ggtgcacaag gtatcggctt
    28381 atatagaact gagtttttat atatgggtcg tgaccaaatg cctacagaag aagaacaatt
    28441 tgaagcttat aaagaagtat tagaagcaat gggcggtaaa cgtgttgttg tacgtacttt
    28501 agatataggt ggagataaag aattatcata cttaaacttg cctgaagaaa tgaatccatt
    28561 cttaggttac cgtgcgattc gtttatgcct tgcgcaacaa gatattttca gaccacagct
    28621 acgtgcatta ttacgtgcat cagtttatgg taagttaaat atcatgttcc caatggttgc
    28681 aacaattaac gaatttagag aagctaaagc tatattatta gaagaaaaag aaaaccttaa
    28741 aaatgaaggt catgacattt cggatgatat agaattagga atcatggtag agatacctgc
    28801 aacagcagca ttagctgatg tctttgctaa agaagtagat ttcttcagta tcggtacaaa
    28861 tgatttaatt caatacacat tagctgctga ccgtatgtca gagcgtgtat catatctata
    28921 ccaaccatat aacccttcaa tcttacgttt agttaaacaa gttattgaag cgtcacataa
    28981 agaaggtaaa tggacaggta tgtgtggtga aatggctgga gatgaaacag ctattccatt
    29041 attgcttggt ttaggtttag atgagttctc tatgagtgca acgtctattc tgaaagcaag
    29101 aagacaaatt aatggtttaa gtaaaaatga aatgactgaa cttgctaacc gtgcagtcga
    29161 ctgtgcaacg caagaagaag ttattgaatt agttaacaac tacgtaaaat aattaattct
    29221 ttcgaa
//
LOCUS       DAKTGS010000016        29107 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.016, whole genome shotgun sequence.
ACCESSION   DAKTGS010000016 DAKTGS010000000
VERSION     DAKTGS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29107
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.016"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            59..628
                     /gene="ahpC"
                     /locus_tag="O0286_000606"
     CDS             59..628
                     /gene="ahpC"
                     /locus_tag="O0286_000606"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCY0169005.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            644..2167
                     /gene="ahpF"
                     /locus_tag="O0286_000607"
     CDS             644..2167
                     /gene="ahpF"
                     /locus_tag="O0286_000607"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCY0169006.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            2297..2539
                     /locus_tag="O0286_000608"
                     /pseudo
     CDS             2297..2539
                     /locus_tag="O0286_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2612..3238
                     /locus_tag="O0286_000609"
     CDS             2612..3238
                     /locus_tag="O0286_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCY0169007.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            3493..3876
                     /locus_tag="O0286_000610"
     CDS             3493..3876
                     /locus_tag="O0286_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169008.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(3942..4523)
                     /locus_tag="O0286_000611"
     CDS             complement(3942..4523)
                     /locus_tag="O0286_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0169009.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            4701..4910
                     /locus_tag="O0286_000612"
     CDS             4701..4910
                     /locus_tag="O0286_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169010.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(4949..5200)
                     /locus_tag="O0286_000613"
     CDS             complement(4949..5200)
                     /locus_tag="O0286_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCY0169011.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            5500..5763
                     /locus_tag="O0286_000614"
     CDS             5500..5763
                     /locus_tag="O0286_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0169012.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(5901..6473)
                     /locus_tag="O0286_000615"
     CDS             complement(5901..6473)
                     /locus_tag="O0286_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0169013.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(6654..7022)
                     /locus_tag="O0286_000616"
     CDS             complement(6654..7022)
                     /locus_tag="O0286_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0169014.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(7391..8002)
                     /locus_tag="O0286_000617"
     CDS             complement(7391..8002)
                     /locus_tag="O0286_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0169015.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            8202..8909
                     /locus_tag="O0286_000618"
                     /pseudo
     CDS             8202..8909
                     /locus_tag="O0286_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8948..9886
                     /locus_tag="O0286_000619"
     CDS             8948..9886
                     /locus_tag="O0286_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0169016.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(10152..10394)
                     /gene="rpsR"
                     /locus_tag="O0286_000620"
     CDS             complement(10152..10394)
                     /gene="rpsR"
                     /locus_tag="O0286_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCY0169017.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(10446..10949)
                     /gene="ssb"
                     /locus_tag="O0286_000621"
     CDS             complement(10446..10949)
                     /gene="ssb"
                     /locus_tag="O0286_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0169018.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(10970..11266)
                     /locus_tag="O0286_000622"
     CDS             complement(10970..11266)
                     /locus_tag="O0286_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCY0169019.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            11510..11701
                     /locus_tag="O0286_000623"
     CDS             11510..11701
                     /locus_tag="O0286_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169020.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(11787..12884)
                     /gene="ychF"
                     /locus_tag="O0286_000624"
     CDS             complement(11787..12884)
                     /gene="ychF"
                     /locus_tag="O0286_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCY0169021.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(12896..13099)
                     /locus_tag="O0286_000625"
     CDS             complement(12896..13099)
                     /locus_tag="O0286_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCY0169022.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(13129..14010)
                     /locus_tag="O0286_000626"
     CDS             complement(13129..14010)
                     /locus_tag="O0286_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCY0169023.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(14167..15012)
                     /locus_tag="O0286_000627"
     CDS             complement(14167..15012)
                     /locus_tag="O0286_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCY0169024.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    15241..15610
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15676..16779
                     /locus_tag="O0286_000628"
     CDS             15676..16779
                     /locus_tag="O0286_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0169025.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            16776..17936
                     /locus_tag="O0286_000629"
     CDS             16776..17936
                     /locus_tag="O0286_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCY0169026.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            17905..19746
                     /locus_tag="O0286_000630"
     CDS             17905..19746
                     /locus_tag="O0286_000630"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCY0169027.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            19743..21971
                     /gene="metE"
                     /locus_tag="O0286_000631"
     CDS             19743..21971
                     /gene="metE"
                     /locus_tag="O0286_000631"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCY0169028.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            22014..22766
                     /locus_tag="O0286_000632"
     CDS             22014..22766
                     /locus_tag="O0286_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCY0169029.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(22849..24030)
                     /locus_tag="O0286_000633"
     CDS             complement(22849..24030)
                     /locus_tag="O0286_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCY0169030.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            24569..25702
                     /locus_tag="O0286_000634"
     CDS             24569..25702
                     /locus_tag="O0286_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCY0169031.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(26069..26698)
                     /locus_tag="O0286_000635"
     CDS             complement(26069..26698)
                     /locus_tag="O0286_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCY0169032.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(26698..27540)
                     /locus_tag="O0286_000636"
     CDS             complement(26698..27540)
                     /locus_tag="O0286_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169033.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(27565..28278)
                     /locus_tag="O0286_000637"
     CDS             complement(27565..28278)
                     /locus_tag="O0286_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCY0169034.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(28275..28478)
                     /locus_tag="O0286_000638"
     CDS             complement(28275..28478)
                     /locus_tag="O0286_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0169035.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            28719..>29107
                     /locus_tag="O0286_000639"
     CDS             28719..>29107
                     /locus_tag="O0286_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCY0169036.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
BASE COUNT         9694 a         5294 c         4371 g         9748 t
ORIGIN      
        1 taagcgcaat caattgatag ttctaattca tacttaaatt cttaggagga agatatttat
       61 gtcattaatt aacaaagaaa tcttaccatt tacagcgcaa gctttcgatc caaaaaaaga
      121 tcaatttaaa gaagttacac aagaagattt aaaaggttct tggagcgtag tatgcttcta
      181 tcctgctgac ttctcattcg tttgtccaac tgaattagaa gacttacaaa accaatatga
      241 agaattacaa aaattaggcg taaatgtatt ctcagtatca actgatactc acttcgtaca
      301 caaagcatgg catgaccatt cagatgcaat tagcaaaatc acttacacta tgattggtga
      361 cccatcacaa acaatcactc gtaattttga tgtattagat gaagctactg gtttagctca
      421 acgtggtaca ttcattatcg acccagacgg tgttgtacaa gcatctgaaa ttaacgctga
      481 cggaattggc cgtgacgcta gtacattagc tcacaaaatc aaagcagctc aatatgttcg
      541 taaaaaccct ggcgaagtat gcccagctaa atgggaagaa ggcgctaaaa cattgcaacc
      601 tggtttagat ttagtaggta aaatctaagg aggcattaca accatgctta atgctgattt
      661 aaaacaacaa cttaaacaac tattagaact aatggagggc aacgttgaat tcgttgccag
      721 ccttggttca gatgataaat ccaaagaact taaagatttg ttgacagaaa ttactgatat
      781 gtcacctaga ctatctcttt ctgaaaaatc tttaaaacgt acaccaagtt tctcagtcaa
      841 tcgtcctggc gaagaaacag gtgtaacatt tgcaggtatt ccattaggtc acgagtttaa
      901 ctcacttgtt ttagcaattt tacaggttag tggtcgtgca cctaaagaaa aacagtcaat
      961 cattgaccaa attaaaaaat tagaaggttc attccatttt gaaacattca ttagtttaac
     1021 gtgtcaaaaa tgtcctgatg tcgttcaagc acttaactta atgagtgtga tcaaccctaa
     1081 catcacgcat tctatgattg atggtgcagt gttccgtgaa gaatctgaaa acatcatggc
     1141 agtccctgct gtctttttaa atggcgaaga atttggcaat ggtcgtatga caatccaaga
     1201 tattctttcg aaactaggca gtacggcaga tgcatctgag tttgaaaata aagaacctta
     1261 tgatgtctta atcgttggtg gtggtcctgc tagtggtagt gcagcgattt acacagcacg
     1321 taaaggttta cgtactggta tagttgctga tcgtatcggt ggccaagtta atgatactgc
     1381 tggtattgag aacttcatta ctgttaaaga aacaactggt tctgaatttt cttctaactt
     1441 agcagcgcac attgatcaat atgacattga tgcaatgaca ggtatacgtg ctacagatat
     1501 cgaaaagact gacgaagcaa ttaaagttac gttagaaaac ggtgctgtct tagaaagtaa
     1561 aacagtcatt attgctactg gtgcaggttg gcgtaagcta aacattccag gtgaagagca
     1621 attgattaat aaaggtgttg cattctgccc tcactgtgac ggacctctat ttgaaaataa
     1681 agacgtagca gttatcggtg gcggtaactc tggggttgaa gcagcaattg accttgctgg
     1741 tatcgttaat catgttacat tattcgaatt cgctagcgaa ttaaaagcag acaacgtgtt
     1801 acaagatcgt ttacgttctt tatcaaatgt tgatatcaaa acaaatgcca aaactactga
     1861 agttgtcgga gaagaccatg ttacaggtat acgttacgaa gacatgaaca ccggcgaaga
     1921 acatctactt aacttagatg gtatctttgt tcaaattggt ttacttccaa acacatcatg
     1981 gttaaacgat gctgttgaat taaacgaacg tggtgaaatt gtgattgatc gtaacaataa
     2041 tacgaatgtt cctggaatat ttgctgctgg cgatgtcaca gatcagaaga acaaacaaat
     2101 tatcatttca atgggcgctg gtgcaaatgc agcattaaat gcctttgact atattatcag
     2161 aaactaaagt ttcattcatt tcactgacag tatcttacat ttgttaagat gctgttattt
     2221 ttttatcccg acataaaatc atgttatgat aaacttaata tatacaacga gatgtgcgat
     2281 aatgcttaaa gtatgtacct ctcgcaaaca aatcaatatg aaaaatgata ttgagaaaga
     2341 aaagtatgtg aacgaaatta ttatgctttt acgtacgata cctacgtcta aattagtaca
     2401 accaattatg cagttattta tagataaagc tatccttaac aacgtccaaa atgatgcatt
     2461 attgatagaa aaagcaactc atgcacgtaa tcatttacgt ctatggtcat tgcaaggtca
     2521 tcgtgacatt ccaaactaat tcaatgttac tctattaaac atgaatacat attttttcaa
     2581 ttaaagataa actaaggagt tatattacat tatgaaaaag agattactac taagtacatt
     2641 tttagcatcg acattaattc ttacaggttg cgcgtccgat caatctgata acgaagatca
     2701 tcatacaagt actggcatcc atgcacctaa aagcgctaaa aaattagaaa caaaagatat
     2761 ctttaactca gataagaaaa atagtgatat tagtgatgca gagatgaagc aagctataga
     2821 aaagtatttg tctgtaaata gcgatatact tgataacaaa tatattatgc aacataaact
     2881 agataaacaa attgacagtc aaacaaaagt gactgaaaaa caagcagaaa cacttagcca
     2941 cttatctaac ttagctgtta aaaacgattt gcatttcaag aaatttgtca ctgaaaacaa
     3001 tattcctaaa gaatataaga aacccgttga attaatgatg aattacttca aagctttaaa
     3061 tagtacaatc gccaatgttg atgaagatat tgaaaagctt agctatcaac cgcaaaataa
     3121 aatcaatgtt gtcgacgtgc caacaaaata tgccggtgat gttaataaaa agcaacaaga
     3181 taaaattaaa gatttcttaa aatctaaagg tatcaagagt gatgttatcg ataaataact
     3241 gaaagccatc attgcgaaat gtaacacatc attacatgag ttttaattca tgacaattct
     3301 ttatctttaa aagtgactaa tcaatttagc taaagtcatc atttgggcat caagcagcat
     3361 attactgttt gatgcctttt taattgcctt aaattctcta tctatgccaa aattcccatt
     3421 agttgacgca tttgactata tacctaacat attccatact tatattgact ctataaacaa
     3481 aggatgatca tgatgaaagt tcgaattact tttattattt tagcaatttt atcgacgatt
     3541 gtttgcttgt tcttagcagc aatgcatcca acaggaccaa acacagtaac ttttgaacag
     3601 ccatatttat tcaccctcaa catcattata atggtattag tcgcattacc ttcacttgtt
     3661 ttagctatat atgattatat gagttttaga atttcttctg ctattttaca atttctaggg
     3721 gctatctctt ggttcttttt atcattgata ttatcgctca cacaatttac accttttaca
     3781 ttagcgtcat ttataacttc aattattttg ttcacaagca caattatcac attagccatt
     3841 ggtggtaagt ctgttgaaaa gaatgattcc ccttaaattc caaatgaaaa aaaggttctg
     3901 aaggccgcta taaaacacag tttttcagaa cctctatact tctattcaat gatatatggt
     3961 ttgcaatttt ctacctttaa atccacagct tctgcccttg aaactttgtt aaaataaacc
     4021 atcaaacaac gaatgacaac ttgatgtgca acaatgacaa tatcatcttt ttgtgtatct
     4081 tcattgacaa catgattcat aaaatgttct acgcgttgat atacatcttc ataactttct
     4141 ccttcaggcg ctttttgtga aaaactatga cgaaagtctt taaagtttgg atcattgaaa
     4201 tatttatcat atttcggatt cgcactgatt tcatctttat attcaccctc aaatacgcca
     4261 agtgaacgtt ctcttaatag aggggtagtc gttgatgcaa tgtcatatgg aaaaatatgt
     4321 tcaaacgttt gctgtgttct taataagtct gaaacatata catgtttaat ctgtttctct
     4381 ttaaaataat cacataaatc gtctgcactt tttgtgccag tatccgttaa cggcacatct
     4441 aattgtccac aaaaataaga tcgaaaatgt ttattatcat aattcgattt tgattcgcca
     4501 tgtctaacta aataaatcgt cataatatta ctccttacct tatgtatttc atatctacca
     4561 taacactttg actactaatt cgatatcaat cttaatattc tattctaaaa aaagaattaa
     4621 ttcatatttt aaattaatga ctctaaaaat agtattgtct ttaatcaagt gatgtgttac
     4681 tatatgggtg tagttcttaa atgactacac agtcttttat attcatgaac ataatactcc
     4741 tggatggcgc aacccaacgt catccttttt ttatttgtca aatactcgct tttttatttc
     4801 caaaatttcc tcatattaat ttattacttt ttaatcctat cttatctaca ttttcaaata
     4861 ttataataca tattgaccat tttcacgcaa ataaaaaaga tgacaactga atgccatctt
     4921 tatttcaatt tccaattgaa tttttttatt atttacgcat agctcttaaa attaacgtta
     4981 cgattgcaat taagataatt gaaccaatta atgctggcaa gatgtaaata cttcctaatt
     5041 caggacccca ttgtccgaat agtttgccac ctacccatga tccaataata cctgcgataa
     5101 tattgcctaa aataccacct gggatatctt tacccataat agcaccagca gcccatccaa
     5161 ttaagccacc gacaattaac attccaataa atccaaacat aattttcagt ctcctttttc
     5221 tatttatttt gcgttattct aagtagtacc ccttatttac aattctaaaa caaattcaaa
     5281 ttatttttat ccaaatattt ttaaaagtag taattgaata tcaattttat tcaatgtagc
     5341 tatcgttatt taaagtctct gtaccgataa tatcatatac atttacatta ttttttctgc
     5401 cgaattcata gcttgattat tttatgttat aggactagaa tatacacata ttattagagc
     5461 atctttgaat tttaaatcaa gaagcgaggt taatgaacaa tgaatatgca tattttatat
     5521 aacttacgaa ctaaacataa tttagaaatt gacgaattag cacagcaatt aaatgagaaa
     5581 tatggtacta aatatgaagc acatcaaatt tgggaatggg agaatcatca ccatgaacct
     5641 aaatttaaag atgccatgca tttagctgac ttctttgatg caccatatga aatgttttta
     5701 gaaagtaagg ttaaagaata tcagaaacat ttagaagaag tcgatattcg catggataaa
     5761 tagatgcaaa taaaccctca caacacgttt ggcatatatc ctttcaaatc tatactggat
     5821 atattacatt acgttgtgag ggtattttat taattaatat gaattaagac attttacaag
     5881 cgttaatgca acgaatcttt ttagtgatct tgctcactct ttaatacttt accgttctta
     5941 gcatcaacag taacttcttg ttttttatta ccttttttca aatcgatatt gtaaacaagt
     6001 ttgccatcat ctttttcaag tgaccattct ttaatatcac catcaaattc tttttgtcct
     6061 tctttaatgg cttttttgta atctatagca tcgctatatt taaagttatc attttcattc
     6121 attgtatctt ctttttcagt ctttttgtta atcacttttt tatttttatc agcaacaagt
     6181 acttctgact cttcaccaga tttttgttgc gtcactttat aagcccattc accattagaa
     6241 ttttcaaatg aaattccttt caacttttgg cctttgtaag tttcttcagc ttttttcaca
     6301 gcatcttctg ggcttgtttt aacatctttt aaagcaataa catcttttgt tgtattagtg
     6361 tcttgattag tatttgactc tgttgatttt gtttcatctt ttggagtatc attgccacat
     6421 gcagtaagca ccaccgctga cattgataac actgctaatg attttaattt cataatatca
     6481 ctctctcttc ctatttttga aactcataac aaaagcttat atgctatata gattgtatta
     6541 ccccttgttt ttaattttat tcataattat tacaaatatt tttaaattaa tcgtcatgcg
     6601 ttactttcgt tcgtactcct tttataaatg aaccatgtaa tataagcatg ctattatcga
     6661 ctcagtttgt ctaatgcttt ctttggtact tcttcctttt caacttcttc aaaagtttct
     6721 acatgatgac ctttatgtgt aattttcaag tatctatgcg gtttaacatc aaatgtagca
     6781 gtatacatta attgcgtctc tttcccttct ttattaaaaa cactttgact ataacttcgg
     6841 agttgatcat ccattctagt agacacctct gtcgttttaa cataagaatc atccttcttt
     6901 actaatgggt taaattgatc tgttatacca tgggagtcta ttgtttttaa aatgaataaa
     6961 gcagcataag cgccaatgat agtaagtaca agatatgtta ttgtttttaa aatcgttttc
     7021 aaaagatcac ctctgacaaa atataaaata ggatgtatct gaaaacaact tacataattg
     7081 taattaaagt gtaagtatta ggagaaaatg atggtatttg aatctgaaag atacgaatcg
     7141 tttttaaact aagttatttt atagagactt tattatgaaa ttgaaagaag ttaattttaa
     7201 ggttgtcgaa tgtaaaaaca tattgattaa ccataaaaga gaaattattt ttgtagattt
     7261 cggcataaac gtcattaaag aattttctat atgatggtgc taatcataac aaagaaagct
     7321 aggcaccttt agatacctaa ctttcaaagt attagcgata aaacactata tatactaatt
     7381 tagcttaatt tcaaacttgt tcaatgtcat taacactttt cacaagtttg aaagttacat
     7441 tttttatatc ggctgtatta atttttacat tttccctatc tttttgtagc tctttatgcg
     7501 actctaatgt atatttacca ccatcttttg tatttatgac gatgttacct ttaggcaaat
     7561 gttctcccat atacaagcca taggaaatat gagcaaatct tatgatatga tccaattctt
     7621 ttaaagtgac aacttcttta ttaagagtaa tattcctctt aggtgtatac actctgccag
     7681 caactgtgta tgttccttct aaatgtatac gaacttggtt ttcatttaaa ggtctctctg
     7741 aataaaccca attcctagat ttattagatt ttccatttat tgtatccttg ctgtatctat
     7801 cgtaaagttt tttaaccaaa gctttttctg aattatttgg tacttcttca actttttgta
     7861 attgtttatc ttgtacactt gaggaatttt gtgttgacgc gtctgctttt ggatatattc
     7921 caaatgttcc cccatagatg atacctagcg atagaataga ttttaatacg attgaatttt
     7981 ttgagtcata ttttttgaac atatttaatt acctccttga tgtaaagctt tatttgctac
     8041 aattataaaa ataatagacg tgttcatgaa ttaaattcat cttaactctt gattaacttt
     8101 aatttgctac cactctgaat ttaataacta taaatcgtct acacataatt ggacaaaatc
     8161 taagagaata aaatttgtta atttaaaata gcaagcaatt caaagttata tgtgtaatag
     8221 ataaaataga tatccctata gtgatgcgtt actagctaaa cataataaca cattagaaga
     8281 taatgaagtt aaggagttac tggattgttt cgactatgta attaagtata aaaatatcca
     8341 acgacaaaac gtaattataa aatggtaaaa gctatggtac agtttcaaat tgctaatgac
     8401 atgcgtatcg gtgagctact tgcaataaag agagtaaata taaactatga agataaaacg
     8461 ctagatatcg acggtaaagt taattggata actgaaaaaa gacgggagca ttcggagtaa
     8521 aggagacaac tgaaagaagt aatagctata aggccacagg gctcactacc caaagcatcg
     8581 acttactaag aacacttatg cttggaatga taagtttatt gatagagagt acatattcac
     8641 aaatacgtct ggtagcccta tcgactcgaa caaaattagc cacattatta aaggggggcg
     8701 ctgatattag ttctattaag aaacctataa cgacgcatac attacatcat tcgcatatat
     8761 ctacacttgc tcaattagga attaacttaa aagcaatgca agagcatgta ggtcattcag
     8821 attataaaaa aaatctagag atatacacac atgttactaa tcagatggcg aaagatatga
     8881 tgaataaatt tgaacgattg gggagttaaa attggaaaaa gatgatacac tagcagaaat
     8941 taagcctatg ctcaattttg atgagcaaat agcgaaatta aaacagatga atatattttt
     9001 taatattatt gacaccgaaa aagcaaatga aattcttaga aaaaataatt acttcttcaa
     9061 actagcttat ttccgaaaaa atttcgaaaa aaagaatggc ggctatttca tagaatttgc
     9121 ttatttatca gatttagcaa ctatagatat gaaattaaga tacacaatgt tgcatttaac
     9181 tttagatatt gaacatagtt taaagtatct agtcttaaaa ctaataacag aaaataacca
     9241 agaagatggt tataaaataa tagatgagtt cttatgtatt gataaatcat atagcaattc
     9301 aaattttgac acaaattcaa gaacaccaga agaagttatg gaaaccaaaa tcaaaaataa
     9361 aaacgaaata ttcaagcata tgaataaacg aggacaacta cccgagaagt tgaataaata
     9421 ctatcaaaat ccacccgcat gggtttgcat tgaattcatg caactaggtc aattcgtttc
     9481 gtttctcaac ttctattaca agaagtacaa tgacgaagaa ttgagagttg ctaatatttt
     9541 aatgccttta gttaaaaata taagaaacaa atcagctcat aaccaaccca tcatagcaaa
     9601 tctaaattat gacagtagat tacctcaata tttatttgaa aaagggaata atataggcat
     9661 atctagaaac atgttcggaa taaaaaattt catagatact ttcgctacgc tagaattaca
     9721 taatcaagtt tgtagtaatg caattatcca agcaagatat cacgatttgg accaacttca
     9781 aaagcgatat aaaagaaacg aaagctatta taataatgca ttagctatca aaagattttt
     9841 tatagcttta gataaaatta ttgacttcaa cagaccaaaa gtataaacta tctagtgagg
     9901 aaagagactt ataggtctcg cgagttattt taattcgtat gcaagaaaaa gaagagctat
     9961 gcattttatt taaaatgcgt agttcttttt ttatgcatct aaattcatat tatttttgca
    10021 atataaacat atctttgtgc aaattccgaa cacaaaacat tcacatcatc cttttttgcc
    10081 ctttttctat accccaaaac acaaaaagcc ccgtaagcct atgcctacgg ggtttgacaa
    10141 taaattatat attattgttc ttctttaaca tatggtaata atgccatatg acgagaacgt
    10201 ttgatagctg tagtcaacat acgttgatat ttagctgaag taccagttac acgacgtggt
    10261 aaaattttac cgcgttctga gataaaacgt tttaataatt cagtgtcttt gtagtcgata
    10321 tgtgtaatac catttgctgt gaaatagcat acttttttac gacgacgtcc gcctcttctt
    10381 ggtccacctg ccatgattgt gtgcctcctt tgataatttt ttcgctttaa tttcgttaat
    10441 ttttattaga atggtaagtc atcatcactt atatcaatcg gtccgtttgc atttgcaaat
    10501 ggattatcag attgtttcgt gtttgatgaa ttattgtacg aattgttttg tcctgattgt
    10561 tgaccaccga atccttgacc gtaatcttgg aattcatttt gttgacgttg gccaccattt
    10621 tgttgcgcat ttttaggttc aaggaattga acgctatcac acacaacttc agtaacaaac
    10681 acacgacgac cttcttgatt ttcataatta cgggattgta agcgaccatc tacaccagct
    10741 aaactacctt tagataaata gttatttaca ttatctgctt gtcttctaaa aacaacacag
    10801 ttaataaaat ctgcttcgcg ctccccttga gcattcgtga acgtacgatt tactgcaaga
    10861 gtgaatgtcg ctacactcac acctgaggga gtggttctgt attccggatc tttcgttaaa
    10921 cgacctacta atacaactct atttagcatt taaacgcccc ctctaattat tacttgtctt
    10981 cgtcttcacg aataaccatg taacgaatga tatcgtcact gattttagct agacgttgga
    11041 attcgtcagt agctttgttg ttatcagatt taacacgtac gatgttgtag aagccatctt
    11101 tgaaatcatt gatttcataa gctaggcgac gtttacccca gtcttttgct tctaaaactt
    11161 ctgcaccttc agtagctaag ataccgttga aacgttcaac taacgctttt ttagcatctt
    11221 cctcaatgtt tgggcgtacg atgtacataa cttcatatgt tctcatttta tatttgcacc
    11281 tccttgtggt ctatacggct tatcaatctt aaaacagata agcaaggaat aattttcatt
    11341 actcacaata aagaattata tcatgcgcca ttacttttta caataataat tcaaactact
    11401 cttcatatca tttttgatat taattcattt gaaactttct atgatatttt taaaaataca
    11461 cttcacaaaa gcgaacatat gttctataat agttgtgagg tggtaaggaa tgaatttaaa
    11521 tattgattgg tctaaagatt ttcaagaatt ccaagagata cttaatagtg gtattcatcc
    11581 tgaatggctt tattgtgcaa aggctaatct tgttttagag cctgcttata ctggcgaagg
    11641 caaacaattt ttcagcactc aagatattat taacgcgagt aaaattattc cattctttta
    11701 ataaattata cgcataatta aaagaggcga acaattgatt ccgcctcttc tacatatata
    11761 ctatttaatt aacttcactc tatcgtttat acattaaatc tgaaatgaac gatatcgcca
    11821 tcttgcataa tatattcttt accttctaat cgttgtctgc ccgcttcttt agcgccactt
    11881 tcgccaccat attgtacata gtcgtcataa cttgttactt cggcacggat aaatccacgt
    11941 tcaaaatcag tatgaatgat accagcacat tgaggtgcag tcataccttg tttaaatgtc
    12001 caagcacgta cttcttgcac accagcagta aaatatgttg ataatcctaa taattcataa
    12061 gttgttctaa ttaatcgatc taatcctggt tcttcgatac ctaaatcttc taagaacatt
    12121 tctttatctt catcatctaa tgtagcaatt tcttcttcaa tttttgcact aataacaatc
    12181 acttcagagt cttcttgcgc tgcatattca cgaatcgctt ttactttatc attatcatca
    12241 tcaccaattt catcttcacc aacattagcg atataaagca tttttttaga agtcagtaat
    12301 tgcgcttgat tcacccattt ttgatcttct tcattaaagt caatactacg agcgggttta
    12361 ccattttcta aagcttcttt aatagttgtt aaaatacgta cttccatttc agcagtctta
    12421 tctttttgac gtgctaattt ttcaattcta ggcaaacgtt tctcaacaga ttctaagtcc
    12481 gctagtacta attccatatt aataacttca atatcatcaa tagggtctac tcgaccagca
    12541 acatgagtta cgttatcatc atcaaatgca cgaacgacct gacaaatcgc atctacttct
    12601 ctaatatgtg ataagaattt attacctaac ccttctccct ttgaagcacc tttcacaata
    12661 ccagcgatat ctgtaaattc aaatgtagtc ggcaatgtct ttttaggttg aaccatttct
    12721 tctaatttaa gtaatctagc atctggcact tctactatcc ctacattagg atcaatcgta
    12781 gcgaatggat agttcgctgc taaagcacct gcttttgtta ttgcattaaa taatgttgat
    12841 ttaccaacgt ttggcaatcc aacgatacct gctgttaaag ccattaatca ttctcctatc
    12901 tttgtgtatc atcatgagat tcgatgattt ttttaagttt tttatcaaac gtttgacgtg
    12961 gaatcataat acttctttga caattttcac atttaattct tatgtctgca cccattctaa
    13021 taatcttaaa acggtttgtt ccacacgcat gttgtttttt catttctact atatcattta
    13081 ttccatattt tgacgccatt aaatatgacc tccatgtatt ataaactact aaacaccgtt
    13141 ttcactatga ttataagcag tcataattgg ttgaggtgtt ttaatacctt cttgtaaaaa
    13201 cattttttgt acttctttgc gaataattcg agctccagca aatccttcac ctggaattgt
    13261 ttctgcagat attctcaata ttactcttgt atcttcaata gcatcaatac caataacaac
    13321 cggatcacta acaaataagt aatatttact acgtaaagaa gtaaatagtt tgtttagttt
    13381 tttatcaaca ttatcaatgt tttcttcgac agacactgga attttaacga tagctgtacc
    13441 atttgtaatt gagtagttcg ttatttcacc catactacta tttggtaaaa ttgttaattc
    13501 tcctgaaatt gtattgattc gtgttgaacg aagtccaatt gatttaactg ttccctctgc
    13561 tacagttgta ccaccgttat ttatcttaac ataatcacct acatcaaatt ggctttcaaa
    13621 tataataaag aatcctgtaa ttacgtcttt aactatagtt tgagcaccaa aacctactgc
    13681 taagcctacg acaccagcac tggcaattac cccttcaaca ctaatgccaa atttacttaa
    13741 aatcgtcgta ataactataa accaaacgat atacttcact acattttgaa caagagatat
    13801 taaagtttta gagcgctttt tgttactctt tttactttta ttttgaatct taaatccctg
    13861 ttcaatcatt ttattcagta ttttaataac tatgagggct acgataatat aaataacaat
    13921 catagcgatt ttagttgcaa tgttttcata tgtttctatt tttgttaatg gctcaaatag
    13981 agatgaaata atattcatga cttgattcat gataagcgtt actcccttct atatgatata
    14041 tcaatattgt attcatattt ttatatactt ccaacctacg aaagtctcaa tatatctaga
    14101 atcaaatgtt cactgattac aatttattta cacttgcaca aagagttatt atataaaatt
    14161 gtgtaactat ttaccatacc tacgatttaa ttgttcaatt attctcacaa aatcttcttg
    14221 tgaatcaaac tcaaatgaga ttttaccaac cgattttttt attgatatat ctactttggt
    14281 accatactgt tctcgcaact gtcgttcttg ctgctttata aatttaggct tagttatatc
    14341 tactttgtct gtttccgact ttgacgaaac attttttaat tcattaacat ggttttctaa
    14401 atatctgaca ctccactttt ctttaacaac ccgtttcgct aacctaagca tttgttgttc
    14461 atctttaatt gccaataacg ttcgtccatg tgcacttgtc agtcgcccat cttttaccat
    14521 gtcagcaatc tttttcggca aatgtaataa cctcaacata ttcgctatat acgggcgcga
    14581 cttactcaat cgtttcgcta cttcttgttg tgtaattttc aaatctgtca tcaaacgttg
    14641 ataactttca gcttcttcaa tcgcatttaa gtcttctcgt tgtaaatttt cgatgaccgc
    14701 cagttccatc atatcttcat ctgttaaatc tttgataatc gctgatacgt attttagacc
    14761 agcaattttc gaagctctaa accttctttc accaactaca atgtaataac cttgaactgt
    14821 ttttctaagc acaattggtt gcaaaattcc atattgctta attgaatctg ccaaatcatt
    14881 taaatgattt tcatcgaaag tttttcgggg ctgatacgga ttcggtttga tatccgatat
    14941 atctatttgc ttaatttgtt catcttttgt tttagtaata cgttgatctt cactttttga
    15001 caattcactc acatcacttt ttatttgata acaacagtta aatatatttt atgtattcta
    15061 aatgagtcag acaaccatta accacacttt atttaaaaaa taattcattt ttcaacttca
    15121 agataccaat tacaatttca caaacaaatt aacttttaaa tcaaaatttt ctgaataatt
    15181 gttgacaaat ctttttttac tctgtaagct aattaatatc ttataacagt ttaatgaaac
    15241 gtaaacacaa taaagaggaa agtaaaacac accctgctta tacagagagt ctttagtagc
    15301 tgagagaaga ttttgaaagc gtgtttgaaa atggccttgg agtgttgatg ccaatatgag
    15361 gtgtctacgg gttcgcccgt tatagcgata cagtattaac attgatgtta aatggcgtac
    15421 tggattcttt acgcacgatt ttttgttaat aagtatggga tagcacatta ctatatcctt
    15481 acttactgac tttaattgtg ataattgttc agtaagcata tttactttta atgcgtactg
    15541 aataaggtta tttcagcgat ggaataacaa ataaaggtgg taccgcgaaa cataagcttt
    15601 cgtccttttt atccgattca ttcgggtacg aaggacggaa gcttttttta ttttttctta
    15661 taggagggtc ttaatatgaa ggatacacag ttagcccaaa tcacattaac cgatgattca
    15721 accggtgcta tagcgaatcc aatccattta tctactgcct acaagcatcc aaaactagga
    15781 caatcgacag gttttgatta tacacgtact aaaaatccta cacgctcaac atttgaaacc
    15841 tgttttgcca aacttgagca tggtattgca tcattcgcta catcaagtgg aatgtcagcc
    15901 attcaattaa tatgtaatct atttaaacct catgatgaaa ttttagtttc attcgattta
    15961 tatggtggca catttagatt atttgaattt tacgagcaac aatacgatat caaatttaag
    16021 tacgttgatt ttacagatta tgaacaagtt gaaaaagaaa tcactgataa aacagttgca
    16081 ttattcattg aaccaatatc taacccacaa atgattgcta ttgatgtaaa gccatactat
    16141 caactttgta aagctaaagg cttattgtca attatcgaca atactttttt aacaccttat
    16201 ctttcaacac cactagcaga aggtgctgat atagtcttac attcagccac gaaatatatt
    16261 ggcggacata acgatgtact agcaggtgtc gtaaccgtca aagatgaatc actcgcgcaa
    16321 cagttgtttg attttcacaa catgactggc gcaacacttt caccaataga tagttatttg
    16381 ttgttacgtg gacttaaaac tttgcattta cgcattgagc gtgcgcaatc aaacgctaga
    16441 aaacttgcta aaaaatgtca gtcacttcaa gcaattgacg aagtactata tagcgggcaa
    16501 actggcatgc ttagtttaag acttaacaag gcctatagcg tcgctaaatt attagaaaat
    16561 ttagacattt gcatttttgc agaaagttta ggaggtactg aaacattagt gaccttccct
    16621 tacacccaaa cacatgttga tatgccagat gctgaaaaag ataaacgtgg cattgatgag
    16681 tatttaatcc gcttatcact aggtgttgaa aattatgaag acatcgaacg cgatatcatc
    16741 caagcattag ataaagctca gattggagag attgtatgac actttcaaaa gagacagaag
    16801 tgatattcga ttggcgtaga ggtgtggaat atcattcagc taacccacca ctctatgatt
    16861 cttcaacatt ccatcaaaca agtcttggtg gcgatgttaa atatgattat gcacgaagtg
    16921 gcaaccctaa ccgtgaactt ttagaagaga agttagcacg attagaacag ggaaaattcg
    16981 cttttgcttt tgcatcaggt attgctgcta tttcagcagt acttttgact ttcaaatctg
    17041 gtgatcatgt catcttaccc gatgatgtat atggcggtac ttttcgcctc actgagcaaa
    17101 ttttgaatcg atttaatatt gaatttacaa ccgtagatac tacaaagctc gaacaaatcg
    17161 agggtgccat tcaatcaaac acaaaattaa tttatatcga aacaccatcg aatccctgtt
    17221 ttaaaattac tgatatcaaa gctgtttcta aaatagccga aaagcatgaa ctactggtag
    17281 ctgtggacaa tacatttatg acaccgttag gtcaatcacc tttattactt ggcgctgata
    17341 ttgtcattca tagtgctaca aaatttttaa gtggacatag cgatttaatt gctggtgctg
    17401 tcataactaa taatgaggca attagtgacg ctctttattt aatacaaaat ggtacaggca
    17461 atatgttatc tgctcaggat agctggacac ttgctaaaca tttaaaaact tttccaatca
    17521 gatttaaaca atctgtcgaa aacgctcaaa aaatcgtgtc atttttaata aagcaagatg
    17581 aaatttcaga agtttattat ccgggactca ctactgctca tttagaacaa gctaaaaatg
    17641 gcggtgccgt tattggcttt cgtttagctg atgagtctaa agcacaacaa tttgtcgatg
    17701 cactgacatt accactcgtt tcagtgagtc tgggcggtgt tgaaacgatc ctttcacatc
    17761 cagcaacaat gtctcacgct gcactacctg aagaagtgag acaagaacgt ggtatcactt
    17821 tcggtctatt ccgattaagt gttggtctcg aagatcctga tgaactcatt gcagacatca
    17881 aatacgcatt aaaggaggca ttcaatgagt caattcctca cacaattgaa agataatgtt
    17941 ttagtagctg atggcgctat aggaaccatt ttatactctg aaggattaga cacctgtcca
    18001 gaagcatata atcttagcca tccagataaa gttgaacgca tccatcgttc atatattgaa
    18061 gccggtgctg atgtcattca aaccaatact tatggtgcaa attttgaaaa gttaaaacga
    18121 ttcggtcttg aagataaagt taaagcaata catcaagccg ccgttcgcat cgcaaaaaaa
    18181 gcagcaaata aagatacgta tatattaggc acagttggtg ggtttagagg tatcaaacaa
    18241 gaggatatca gcttacaaac tattctttat catactgaaa ttcaaataga caccttaatt
    18301 gaagaaggcg ttgacgcgct acttttcgaa acgtattacg acctagaaga gttaacaaat
    18361 gtcatttcac gaacgagaaa gaaatacgac attccaatca ttgctcaatt aaccgcttca
    18421 aacacaaatt acttagttaa tggtcaggca atcaatgaag gattaaaaca actcgttcaa
    18481 tgtggtgcaa acatcgtggg actcaattgt catcatggcc cgcaccatat gcaagagtct
    18541 ttcacacata ttgaattacc agagcacgca ttcttatctt gttatccaaa tgccagctta
    18601 ttagatattg aaaatagtga atttaagtat agtgacaatg cacaatattt cggtcaagtt
    18661 gctcaaaatc taattcgcga aggtgttcgt ttaattggtg gttgctgtgg tacaacgcca
    18721 gagcacatca aatttattaa agaatctatt cagacactta aacctgttaa tgacaaaaaa
    18781 gtgattccga taccaacgaa agcacttttc aatccatctc aaaataaagt tagacaatca
    18841 ttaacatcta aggttcaaga acgtccaacc gttattatcg aattggatac accgaaacat
    18901 ttagacacgg atagattttt tgaaaatatc gctaaacttg ataaagctaa tgtagatgcg
    18961 gtaacactcg cagataattc attggcaact gtcagaatta gcaatattgc tgctgctagc
    19021 ttaattaagc aatattacaa tattgaacca ctcgtacata ttacatgtcg agaccgaaac
    19081 ttaatcggct tgcagtccca tttacttgga ttatcgctca ttggcgttaa cgaaatatta
    19141 gccataactg gtgatccttc aaaagttggt cacttaccag gtgcaaccaa tgtctatgat
    19201 gttaattcta aaggattaac tgaactcgct ctaagattta atcaaggtat taacactgac
    19261 ggtgatgcgc tgaagaaacg tacacacttc aacatcgctg gcgcctttaa ccctaatgtt
    19321 cgtaaattat atggtgccgt caaaagatta gagaaaaaga tagaaagcgg aatgtcttat
    19381 tttataacac aacccgtgta cagcaaagag aaaatcattg aaatttacca tgccactaag
    19441 cacttgaaca aaccattttt cataggcatt atgcctatcg caagttacaa aaacgcactc
    19501 tttttgcata atgaagtgcc aggaatcaag atgtcagatg aaattttaca acaatttgaa
    19561 gcagttaaag atgataaagc caaaacacga gaactaagtc ttaagctttc aaaggattta
    19621 atcgatactg ttcatgaata ttttaatggt ttatacatta tcacaccgtt tcaaaatgtc
    19681 gaagattcat tagaacttgc agcatactca aaatctatta ctgctcacaa ggaggcaata
    19741 ttatgacaac aattaaaaca tcaaacttag gattcccaag attaggtaga aaaagagaat
    19801 ggaaaaaagc catcgaaagt tattgggcaa agaagatttc taaagaagaa ttagatcaaa
    19861 cattaactga tctacataaa gaaaatttat tattacaaaa gtactatcac ttagatagca
    19921 tcccagttgg tgatttctct ttatatgacc atatattaga tacgtcatta ttattcaaca
    19981 tcatccctga acgtttccaa ggaagaacta ttgatgatga tttattattt gatattgctc
    20041 gcggtaataa agaccatgtt gcaagtgcac ttataaaatg gtttaacact aactatcatt
    20101 acatcgtacc tgaatgggac aatgttgagc ctaaagtgag tcgcaatgta ttattagatc
    20161 gctttaaata tgcccaatct ttaaacgtta acgctcaccc tgtcattgtt ggtccgatta
    20221 cttttgttaa attatcaaaa ggcggtcacc aaacttttga agaaaaagtt aaaacgttat
    20281 taccacttta taaagaagtg tttgaatcat taattgatgc aggtgctgag tacattcaag
    20341 ttgatgagcc aatcttagtt acagacgaca gcgaaagcta tgaaaatatt acacgtgaag
    20401 cttatgacta tttcgaaaaa gctggtgttg ctaaaaaatt agtcattcaa acatactttg
    20461 aacgtgcaca tcttaaattc ttaagttctt tacctgttgg tggtataggt ttagactttg
    20521 tccatgataa tggctataac cttaaacaaa ttgaagctgg agattttgat aaatcaaaaa
    20581 cattatacgc tggaattatt gatggtcgta atgtatgggc aagtgacatt gaagctaaaa
    20641 aagtcttaat cgataaattg ttagcacaca ctaatgaact tgtcattcaa ccatcatctt
    20701 cattattaca tgttccagta tctttagatg atgaaacatt agatacaagt gttggcgaag
    20761 gcttaagctt tgcaactgaa aaattagacg aattagatgc attgcgtcgc ctatttaatc
    20821 aaaacgatag tgttaaatat gataaattaa aagcacgtta tgagcgtttc caaaatcaat
    20881 cattcaaaaa cttagattat gatttcgaaa gcgtgcgtac ttcaagacaa tcaccattcg
    20941 cgcaacgtat tgaacaacaa caaaaacgtt tgaacttacc ggatttacca acaacaacta
    21001 ttggatcatt cccacaaagc cgagaagttc gaaaataccg tgcagattgg aagaacaaac
    21061 gcattacaga cgaagcatat gaaacattct taaaaaatga aattgctcga tggattaaaa
    21121 ttcaagaaga cattggctta gatgtattag ttcacggtga atttgaacgt aatgacatgg
    21181 ttgaattctt cggagaaaaa ttacaaggtt tcttagtaac taaattcggt tgggtgcaat
    21241 catatggttc acgtgccgta aaaccaccaa tcatttatgg tgatgtaaaa tggacagcgc
    21301 ctttaactgt tgatgaaaca gtttatgcac aaagcttaac agataaacca gttaaaggta
    21361 tgttaactgg acctgtaaca attctaaact ggtcatttga acgtgttgat ttaccacgta
    21421 aagtcgttca agatcaaatt gctttagcaa tcaacgaaga agtattagca cttgaagctg
    21481 caggaatcaa agttatccaa gttgacgaac ctgcattacg tgaaggctta ccattacgct
    21541 ctgaatatca cgaacaatat cttaaagatg ctgttttatc atttaaactt gcaacgtctt
    21601 cagttcgtga tgaaactcaa atccatacac atatgtgtta ttctcaattc ggtcaaatca
    21661 ttcatgctat tcatgactta gatgctgatg ttatttcaat tgaaacatct cgtagccatg
    21721 gtgatttaat taaagacttt gaagatatta attatgattt aggtattggt ctaggtgtat
    21781 atgatattca tagtccacgt attccaacaa aagaagaaat tactacagca atcaatcgtt
    21841 cactacaaca aatcgaccgc tcattattct gggtaaaccc tgactgtggt ttaaaaacgc
    21901 gaaaagaaga agaagttaaa gatgcattga ctgtgcttgt gaatgctgtt aaagctaaac
    21961 gccaagaata atcgcagcat ttatcatata ataaactaaa aacgggtgat tttatgacac
    22021 aatatccttt atggcaacaa ttaaatactt taaaacaagc aacttgggtt gatttaacgc
    22081 atacatttga tccggaaata ccacgtttta gtgagtttga aaaaggcgaa gtttcaactt
    22141 tattcacggt taaagatcat ggtttctatg tgcaacgttg gaatattgta acgcaatatg
    22201 gtactcacat tgatgcacca attcactttg tcgagaataa acgttattta gaagacattg
    22261 atttaaaaga attggtattg ccgttaattg tattagactt ttcaacagaa gtagcgaaca
    22321 acaatgattt catcgtaaca cgtgcgcata tagaagcatg ggaaaaagag catggtacta
    22381 ttgaacctgg cacttttgtt gcacttcgta ccgattggtc aaaacgttgg cctaatattg
    22441 aaaagtttga aaacaaagat gcgaacggac aacaacatgc accgggttgg ggattagatg
    22501 ctttgaagta cttaattgaa gaacgtcgtg ttgaagctgt tggtcatgaa acattcgata
    22561 ccgatgcatc tgtggacgtt gtaaaaaatg gtgatttagt tggcgaacgt tatattcttg
    22621 gtcaggataa ataccaagtc gagttattaa ctaatttaga tcaattacca actcgtggcg
    22681 ccatcattta cgctatcagc cctaaaccaa aagatgcacc tgggttccct gttagagcat
    22741 ttgcaattaa accatcaaat gactaatatt acccaataaa tccataaaca aataaaaaca
    22801 ggttatgcca acatgtaatt aatactgttg tgataacctg ttttcttatt atttatactt
    22861 tgatacaact gcagcgatag cttgaccgcc accaatacac aatgatgtta aaccagtttc
    22921 aacttcatca ttcagttgat gcaataatgt cactaatact ctagcaccag atgcaccaat
    22981 aggatgtcct aatgcaatag cgccaccttt aacattcacc ttttcaggag gtaattttaa
    23041 ttcacgatca acagctaatg attgtgctgc aaatgcttca tttaattcaa atacatcaat
    23101 atcgcttaat tcttttttac tacgtttcaa agccttttct acagcgccaa ctggtgcaat
    23161 acccataata gaaggatcta caccatgact tccaaagcca tcaagcactg ccaatggttc
    23221 gatatttaat tctttagctt tgtcttctga catgactaac atcatcgcag caccatcatt
    23281 gattcctgat gcattacctg ctgtaactgt accgtctttt ttgaaagctg gtcttaatcg
    23341 acttaatttt tcgactgata cattttcacg tacaccttca tccttagtga ctacgattgg
    23401 ttcaccttta cgttgaggaa tcgatactgg aactatttca ctatcaaatt caccattttg
    23461 ctgtgcacgt actgcttttt gttgtgagtt tacagcaaat gtatcttgtt cttctcttga
    23521 aataccatat tgctctacta aattttcagc agtaataccc atatgatatt gattaaatac
    23581 atctgttaaa ccatcatata ccatgctatc aaccattgat tgatgtccca ttttaaaacc
    23641 aaagcgactg ttgttgacaa gcattggtga ttgagacata ttctccatac cgccagctag
    23701 cacgatgtca ttttcaccag tcacaataga ttgatatgct aattgaatcg actttaaccc
    23761 agaaccacat actttattca ccgtaaatgc aggtactgtt tctggcaagc cacctttcat
    23821 agcagcaatt cgtgctggat tttgtccttg tcctgcttgt agtacgttac cgatgataac
    23881 ttcatcaatc tcacttggat tcaaacccgt ctctttaata atatgttcta ttaaagtcgc
    23941 acctaaatca taggctggca cgtctttaaa cgcacctcca aaaacgccaa taggtgtcct
    24001 gtatgctgct gctaatacga ctctcgtcat gtcatattca tctccttatg atatatacaa
    24061 ttttattact aatacgtcta atgaaccaat acgccactaa catccaattt atgaaaccaa
    24121 ttgctacgta tgttacattt tcattcttgt tatgcattta acaaatcata ttatacaatg
    24181 atgtaatttg tttgggcaat cggaaacgta aacggtttca ctttaacatt tcaccctctt
    24241 aaatatgctc tatcatcctc atctatagta caaaattatg ctttgattta ccacaaatta
    24301 tcattaatca tttcgcgtaa tgactctaat taaattctat ctatcattgc atcgctattc
    24361 acagctggtt tcaccatcaa tttctcatca attttttatt accatgacgt ctccagtcaa
    24421 atattaaatc gctgatgatt gttaattccg caattacctt gcagtcacct ctccttccac
    24481 ttcatcgcca aatctgcccc tattcactag aaaaaataaa tagtatactt agtaaattaa
    24541 ttacacatct taaaggagtt aaaattttat ggttaatgca ttaacaccaa aacatttagc
    24601 tgaaaagcgt gcaggattcc atgagttgtt ctttgattta attttcgtct atgccattca
    24661 aaaaattgca catgtgattt taacaacaca aaacggttcc atttcagctg atttattttt
    24721 caaatacatc gtcatgagtt tgttcttatg gttaatgtgg tctcatcaaa cattttttac
    24781 aaatcgattt ggtcaagtta cattcaaaga cgtctcgttt atgatgttta atatgttcat
    24841 catggtcttc ctatctaaca gcctatatcc ggactttgaa aagacattct tcccattctt
    24901 cttatgcgtg gcaattatgt atttaagtat cggattacag tatttattac atatacgtac
    24961 agggttagat tatggcgata agcgtacatg tcaagctttt gcgagcgtag cctttgtgat
    25021 ttcattttta tcatttctat cattagtact accacaaagt atccattaca tccctggatt
    25081 tttaggcgta ttcattgcag cgacaggact tattccattt cagaaatatc ttgtattatc
    25141 acctgttaat atgatgcatt tagtagaacg tttttcgtta ctaacaatta ttatctttgg
    25201 cgaggtactg gttggtttag cttcgtcatc atttagcatt gatcatttta gttacattta
    25261 tatattccaa tttatgattt taattagtct tttcggtgta tattggatca ttactgaaaa
    25321 ttatattaat cataaattaa catctatcgg tttccgttta tcgtatacgc atctattaat
    25381 caatatcgct ttaggtgtga ttaatgctgc aattgttttt agtaataaca ataaactaaa
    25441 tgacttgttt gaaattaata tgatgtatat cagtgtttta atattctata ttggattatg
    25501 gttaatcacg ccttatttcc ataatgaact gacaaacgcg aagtatattt catattcact
    25561 aggtatactc gttatgtctt atattgtcag ccttttattt aaaggtcacg accatgtaat
    25621 gattatctca gtatcagttg caaccttctg catcatgctg atttatttta aaaatcaacg
    25681 tctacgtaat aaacatgaat aaatagtatt tacctcggtc aaagtattgg aactgaatcc
    25741 aaaagtgtcg aacttcacat tatacttttg tgacaaactt cagttatgct acatgatcga
    25801 ggtttttaaa tatttccgag gaaatcgtta gcctatacaa tcatcaaatg atacttataa
    25861 ataacatata tcctctaatc cccactatct aaaaggaaat aattcccaca caacataacc
    25921 acctcaaaac aaataaatca atatagatat gtcacctata cttatccccc acatttcatt
    25981 ccacaccaaa aagaacagat gacaatcgta aagatttatc acctgctcta aatattattt
    26041 caaatactat catcaccaaa atcgacaatt atagttcttg tttgttaaaa attttaatag
    26101 acaatatata agaaacgctc gttataacta taccaatgac cgcatatgct atgacaacta
    26161 gccatccttc agtgctaatt ttttcaaatt ctaatgcaga tgcaccacta accataccat
    26221 atataaagaa aaagaccacg aaagaaataa gtagtagtat agatgcacat atcataatga
    26281 cattggagtt ttcagcgcca aatttaaatg tcaatgggaa aatgatagag tatgccccta
    26341 tgataccaaa actcattaaa cctgacataa taccaatcat cacactttgg gtcacaatcg
    26401 tagtcacaac taatccaatc attaaacttg caccgaataa gattaaataa aaggcaaagt
    26461 atgacttaat ataatcacta cgtttaaccg gtaaagtaga tacataatac atccatcttg
    26521 agtctttttc atgtttaata ttatcagtaa taggtgtgat taacataacc ccagccattg
    26581 ccgaactcat caacggatta aatactgcaa agtatcctgc agctataata gcgactataa
    26641 aataaatata tgtttgcttt ctcgttgcat aaaaactact taggaacata cctttcatta
    26701 tacttcacct cgcattatga tttttgtagc gtcatcaatg tgttttaaag gctctgcatt
    26761 ttcaatttct gcataatctt taactaaaat ttgatatttc ccctttgaca atctagaagc
    26821 aatgattaaa tgcttaggaa tttcaacatc tttatcttct gtcgtaacaa ttccatattg
    26881 tgctaaaagt atatctttct gttcagttaa aatcattcgt ccatctttca taaacactaa
    26941 tttatcagct aaatgttcta tatcttcaga aatatgcgat gatattaaga tgcctccacc
    27001 ttgtgcgaca aaatcttcta gtatttccat tacttcttct cttccagaaa catccatacc
    27061 tgcagttgct tcatctaaga ttaataactt cacatcatga gaaagcgcaa tcgttaaagc
    27121 tatcttcatt cgcatccctc ttgaaaaagt tttaatttta gtttgtagtg gtaactcgaa
    27181 atatttgatt aaatcaaaga atttttgact attccaagtc atataaatag attgaaatac
    27241 tttatcaata tctttaatag tcaatttatt aggcactctc aaatcatcaa acactacacc
    27301 tatgtgttct ttatattcga catcatccac ggtatgttct tctccaaaaa atgtgatact
    27361 accgttatct ttatgtcgat tgcctactag aatattaatt aatgtcgatt tacctgagcc
    27421 atttttccca attaatccaa ctgtctcatt agtagaaatc gttaaagatg catcttgcaa
    27481 tttaaaatcc gaatctttgt attgtttgtt cacactatct acgtttaaca atgttgtcat
    27541 atccatgctc ctctttgttt aattttaata aaaacgtctt ctcttcaata agtaactaaa
    27601 ggcgttatat atgaaaatag caatgataag tagcaaacta aagctttgat taattccagt
    27661 agtaattgaa taaagcccaa ttactacaat ggctagtatc aatattgata agttgatcgc
    27721 atatgtttta aataatgcaa ttaattctat atggcgttca ccttcatcca atatttccaa
    27781 tcgcttttca gtgtaattct tatctgcaat ttttggtatt ctgtcatcaa accttctatt
    27841 aaacagtgta aattgtgtat tgaaaatagc actagcaaaa aatggtatta aaaagaatag
    27901 tattgcattt gctgcagcgt gccctaccac aaaaataagc aatactaaaa aagcaataac
    27961 tgtctgcaga atacttaata tacttccatt taaaacataa cgattcgcaa gcaactcata
    28021 ttgatctgtt gcatcgatat ctacctcttt atctacaagt tgcttatact tcaatgcacg
    28081 tctttgattc atcaaaacaa tggcttctac caatatgata ataatcgtcg caacaatcga
    28141 tattacaacg acattgttat atgtcgcaaa cgtcaagttc tcaataccaa atccatcaaa
    28201 attacctaaa attccaccta tgataccccc tacaagtcca cccagtaaaa gatatccgat
    28261 atatcttagt attttcatgc ctcatcctcc tcaataataa aaacagtttc caccgtttca
    28321 ttgaaaatgc gagcaatttt tattgccgtt aatactgatg gcataaaatt gtttcgctca
    28381 attagcgata tggtttgtct tgaaacgccc gcttgtttag caagttgcgt ttggtttaag
    28441 ccatctcgtg ctcgtaattc tttcaatcga ttacgcacat cgcatcaact ccttaattac
    28501 actgtaaatg atatgcgtct ttttgacaac tatatttgtc aaatctacac caaaaaatat
    28561 gattatccac ctatgtatga cattttgaaa caaacacctc aacgcctaca agtcataatt
    28621 gtttactttc gttacacctt cctgcataat taacagcatt ctaattttag tatgatgcac
    28681 gcattttcac taaatcaaac cattcaaagg agactattat ggcatttaca ttatctgcaa
    28741 ttcaacaagc acatcaacaa tttactggtg ttgactttcc aaaactattc aaagctttta
    28801 aagatatggg gatgacttac aatatcgtca acattcaaga tggcactgca acatacgtac
    28861 atcaatcaga agatgatatc gttacgtcat ctgtaaaaag taatcatcct gttgctcaaa
    28921 aatcaaacaa aacaatagtt caagacgtct taactagaca tcaacaaggg caaacagatt
    28981 ttgaaacatt ttgtgatgaa atggctgaag ctggcattta taaatggcat atcgatattc
    29041 aagcgggcac ttgtacttat atcgacttgc aagaccaagc tgttatttca gaattaatcc
    29101 ctcaata
//
LOCUS       DAKTGS010000017        28660 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.017, whole genome shotgun sequence.
ACCESSION   DAKTGS010000017 DAKTGS010000000
VERSION     DAKTGS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.017"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            38..262
                     /locus_tag="O0286_000640"
     CDS             38..262
                     /locus_tag="O0286_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCY0169037.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            279..980
                     /gene="tatC"
                     /locus_tag="O0286_000641"
     CDS             279..980
                     /gene="tatC"
                     /locus_tag="O0286_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCY0169038.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(1072..2784)
                     /locus_tag="O0286_000642"
     CDS             complement(1072..2784)
                     /locus_tag="O0286_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCY0169039.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(2765..3994)
                     /gene="efeB"
                     /locus_tag="O0286_000643"
     CDS             complement(2765..3994)
                     /gene="efeB"
                     /locus_tag="O0286_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCY0169040.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(3991..4845)
                     /locus_tag="O0286_000644"
     CDS             complement(3991..4845)
                     /locus_tag="O0286_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169041.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(5113..5658)
                     /locus_tag="O0286_000645"
     CDS             complement(5113..5658)
                     /locus_tag="O0286_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0169042.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            complement(5738..6034)
                     /locus_tag="O0286_000646"
     CDS             complement(5738..6034)
                     /locus_tag="O0286_000646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169043.1"
                     /translation="MIGNLDINQSNRHYNAYWRGPNKEADEKSAYNNVQVGDGPQHRE
                     IGSVISTDNASWRGPNIENFEKKFYKQCKLAGPQHREFRKEILQAMQVGEGQQI"
     gene            6132..7127
                     /locus_tag="O0286_000647"
     CDS             6132..7127
                     /locus_tag="O0286_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCY0169044.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(7187..7753)
                     /locus_tag="O0286_000648"
     CDS             complement(7187..7753)
                     /locus_tag="O0286_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0169045.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(7767..8828)
                     /locus_tag="O0286_000649"
     CDS             complement(7767..8828)
                     /locus_tag="O0286_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0169046.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(8842..9768)
                     /locus_tag="O0286_000650"
     CDS             complement(8842..9768)
                     /locus_tag="O0286_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0169047.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            10076..11434
                     /gene="glpT"
                     /locus_tag="O0286_000651"
     CDS             10076..11434
                     /gene="glpT"
                     /locus_tag="O0286_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCY0169048.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(11517..11957)
                     /locus_tag="O0286_000652"
     CDS             complement(11517..11957)
                     /locus_tag="O0286_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCY0169049.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(12061..13416)
                     /gene="mepA"
                     /locus_tag="O0286_000653"
     CDS             complement(12061..13416)
                     /gene="mepA"
                     /locus_tag="O0286_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCY0169050.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(13523..13942)
                     /gene="mepR"
                     /locus_tag="O0286_000654"
     CDS             complement(13523..13942)
                     /gene="mepR"
                     /locus_tag="O0286_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCY0169051.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            14154..16109
                     /locus_tag="O0286_000655"
     CDS             14154..16109
                     /locus_tag="O0286_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0169052.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            16114..16557
                     /locus_tag="O0286_000656"
     CDS             16114..16557
                     /locus_tag="O0286_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0169053.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            16559..16843
                     /locus_tag="O0286_000657"
     CDS             16559..16843
                     /locus_tag="O0286_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0169054.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            16858..18204
                     /locus_tag="O0286_000658"
     CDS             16858..18204
                     /locus_tag="O0286_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCY0169055.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(18297..19322)
                     /locus_tag="O0286_000659"
     CDS             complement(18297..19322)
                     /locus_tag="O0286_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCY0169056.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(19637..20659)
                     /locus_tag="O0286_000660"
     CDS             complement(19637..20659)
                     /locus_tag="O0286_000660"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0169057.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            complement(20637..21581)
                     /locus_tag="O0286_000661"
     CDS             complement(20637..21581)
                     /locus_tag="O0286_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCY0169058.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            complement(21571..22371)
                     /locus_tag="O0286_000662"
     CDS             complement(21571..22371)
                     /locus_tag="O0286_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCY0169059.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            complement(22372..22704)
                     /locus_tag="O0286_000663"
     CDS             complement(22372..22704)
                     /locus_tag="O0286_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCY0169060.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(22738..23739)
                     /locus_tag="O0286_000664"
     CDS             complement(22738..23739)
                     /locus_tag="O0286_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0169061.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(23853..23945)
                     /locus_tag="O0286_000665"
     CDS             complement(23853..23945)
                     /locus_tag="O0286_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169062.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            24041..25240
                     /locus_tag="O0286_000666"
     CDS             24041..25240
                     /locus_tag="O0286_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0169063.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            25313..26140
                     /locus_tag="O0286_000667"
     CDS             25313..26140
                     /locus_tag="O0286_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0169064.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(26382..28454)
                     /gene="lip2"
                     /locus_tag="O0286_000668"
     CDS             complement(26382..28454)
                     /gene="lip2"
                     /locus_tag="O0286_000668"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCY0169065.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
BASE COUNT         8782 a         5659 c         4354 g         9865 t
ORIGIN      
        1 gtataaagta aatactatat actacttatg agtgaggttg attatcatga taactaacac
       61 ttttatttta ggcatcacag gcccaacaag tcttgtcgtc attagcatta tcgctttaat
      121 tatttttggt ccgaaaaaat taccacaatt tggccgtgcc atcggttcta ctttaaaaga
      181 atttaaatct gcaacagaag atttagataa agagtctcac gatacaccca gtaaggaatc
      241 gaaacaacag cgagagcaat agcactgacc acaccttact ggttcacttt agcgaactac
      301 gccatcggtt agtaaaaatt ttattgtcgt tcgtcattac ggtcatcgtc gtatatgttt
      361 catcattttg gtggatgaca ccattcataa cgtatattac ccgtgcacat gtgtccttac
      421 atgcattttc attcacagaa atgatacaaa tatacgtgat gattatattt ttcatcgcgt
      481 tttgtttcat ttcaccagtc atgttttatc aattgtgggc attcattgca cctggattac
      541 ataataatga acgtcaattt atttataaat atagtttttt cagcgtgtta ttattctgtg
      601 ctggcgtcgc ttttgcattt tatgttggct ttccaatgat cattcaattc gcgttaaaat
      661 tatcattaac tttaaacatt tcgcctgtca ttggctttaa agcatatttg attgaactca
      721 tacgttggtt attcacattt ggtcttctat ttcaattacc aatattattt atgggtcttg
      781 caaaattcgg tcttatagat accacatcat tgaaacatta tcgtaaatat atttatttcg
      841 cttgctttgt attagcaagt atcattgcac cacccgattt aacattaaat atattactca
      901 cattgccact catattgctc tttgagttca gtatgttcat cgttaagttc acatgccgag
      961 gcaaaccgcc aacacattaa ccttatacga atcaatgctg tgattatcgt tatactgcaa
     1021 taccgaatat ttccaatata gcaacaaaaa aacaggcact cggcctgttt tttatttttt
     1081 aaatttaaag attaagatag caactaccat aatataagca ccttgtgcta tcaatggttc
     1141 ataacttgga taaaagccca accagttaat cgttgggaat ccttcaataa catgtcttgg
     1201 catcgcacct aataattgta acttttgaat acttacgcca agcattttga atcccataat
     1261 aaaaataaag atcgacaaca ctctaaatat atagaaaata ggtattaatt taactataaa
     1321 tctaaataat aatgcaaaga tgattaaaat aacgatagct aaagcaatac caataataaa
     1381 atctttggtc gctagctcac ctatcatccc catatagaaa atgataacct cgacaccttc
     1441 acgcaacaca gatattaaac caatcgtcgc taacaatacc aaattaccat tactaatcgc
     1501 attagcatac atatttttaa tcatgtcatt ccaacgtttt gcatttgaac gtttgtgcat
     1561 ccaaacacca acgataaaca ttaatatgac cgcaacgata cctaatcccg cttccatact
     1621 ttcacgaaga atgccactat tccctaaagt ttctacaaac gtaattgcta agataatact
     1681 cagtacaagt ccggcaattg caccaccaat cacacttgca gtccctttct tatcttttac
     1741 attacgcgtc atggtagtca atgtcattac aattaacaac acttctagcc cttcacgtaa
     1801 aaagataatc atcacatcga cgaagctata actatggcca acaacctctt taatttggtt
     1861 atttaaatct actaaaccat ctttcacatg tgctttatta tgttcgtcta atacactttg
     1921 ataatatggt attttatctt caattttcgt atacaaagca ccgtctttag tttgaatttg
     1981 accttcaaca tacggccaag tttctataaa atgtgtaagc gcagcatcag catccgacaa
     2041 ttgattgtcg tcgatagctt taatcgcctt ctctaacgca tcatttaatt gtgatacatg
     2101 gtattgatca tttgcagacg tattactttt tttatcgaca tgatcaatat ttgatttaaa
     2161 agttgtccaa gcatgtgaca cttttgccgt atctaatggt gacttatgaa ttgcaattct
     2221 aagttgtaat aatgcgactt caatttgtcc atattgattt gcgtcataat tgcgaatcac
     2281 tgtttcatta cttgtccaaa tctgattcaa actattgttc aaagattcta attccgcttt
     2341 atttttatct ttaatcgctt ttgtcatcgc agcatcttta gcatcgactt gctgttgcaa
     2401 tagtttaatt ttagaacccg catctttact agccaatttc tcttcataag caattaatga
     2461 cttcgttaat tgcgaaagtg tatctttttg attatcattc gcttttgcat cttcaagctt
     2521 tctcacatct gatttgacag cattactttc actattatct tcaagcgata atttcttaac
     2581 tgcacttacc acttgctcaa ttgctttctg cttattgtca ttcgatatcg aattattaga
     2641 aagtgcagat ttcgcatccg ttatcacact atatacatca ctaatacttt gttgttctgc
     2701 tgcctgactt ttcagtaacc caaagctaca caccatagca gcagttatta gcattgctac
     2761 aaatttagtc aaataatgtt tcaccaaggt atcctccctt actaacacct ggtaatacta
     2821 aaaatgaagc agaacctcta tgtgtaatat attcatttaa tttatcatta ctacctaaat
     2881 tattttgtat atcgataaat tgttttgtcg ctttttgaaa agcaataaaa agtaagcctg
     2941 tttcgaagtt acctgtgcgg tcatccgtac catccacata attaaaggct ctacgtaaaa
     3001 ttgacgtatt tgcttctttc gctagcctcg tatgggcatc tttatcaata atatactcgc
     3061 catgactatc tttcgctttt aagtcaattt catcaaactc tttcccacct gttaacggtg
     3121 caccactatg tcgtttccga ccaaatgtag cctcttgttc ttccagcgca gtacgatccc
     3181 acgtttcaat gtgtatttga atacgtctga caacgcaata agttccatgt ttcgcccatc
     3241 cgtcatcaat aaacacataa tctttaagtt gattattctt cctaggatta attgttccat
     3301 ctttaaatgc cattaaattt ctaggtgttt ccttaccttt agcagagata aaaccagatt
     3361 gcgcccaacg taccttaaca atatctcgaa atggacgaac taaattatga accgcatgaa
     3421 aggatacttg cgaatcattt gagcatgctt gaatcataat atcaccatcg ctgtaatcgt
     3481 ctattaactg atcattcgga aaatgcggta aatctttaaa ggcatcggga attttactag
     3541 ataacccaat tttcttcatc aaagacttac taatcccaaa ggtaatcgtt aacttgcttg
     3601 cacctaatcc tatcgattca ccggtatcta ctggtggcat taaaggattg ttactcggct
     3661 tgccaactgt atcaccatcc atcatacgat cagccataac cgtccacttt ttaaacattg
     3721 ccttaattgc atctctatct tttgacttca aatctaacgc aacgaaattg catgtttttt
     3781 gcgtgggtgt ggtaatgcct ggttgcactt taccataaaa ttcatacgca tctttttccg
     3841 gatcttctgg tgtattgaac attgatttga aagaccacat gctaccaaca ccacttgcgc
     3901 caattgcaac accggcaccg ccaataccta acattttcaa aaatgtacgt cttgaaaatt
     3961 gcgtactatc gttaacttgt tcataatttg tcattcagta atcacagcca ttttacttag
     4021 tggctctcct aatgcattaa cagcatccgc aaatgcttta cggtctttct tcgatacttt
     4081 ttcaaatgac tcataaccgc cgttgttatc tttatattta tccaataatt gattcacttt
     4141 atcgaagttc atttggatat catcacttaa ttttttatct tttttctcta aaataggttt
     4201 aaataagtca taaatttttt gtgcgccttc aacgttcgct ttaaaatcat ataaatctgt
     4261 atgtgaataa atttcttctt cacctgtgat tttagaagtt gcaacttcat ttaataggtc
     4321 aacagaacct tgtaacatta attttggtat gatatctaat gtatcagctt tggcatgcaa
     4381 ttcttttgca tctttcaata attgttgtgc atctttttta gtcacatcat caattttctt
     4441 atcttcgtat aatgcttttt caatcttatg atatcctgac cattcttttt cctttttctc
     4501 ttctttcata tctgcaagac gtgcatcaat tttaggatct aaatctccaa atgcttctgc
     4561 aactggttca gagcgttcat aatacatacg aacttttgga tataacgctt ttgcttgggc
     4621 catatcatta ttttcaatcg ctttaacaaa tttttctgta ccttttaaaa attcatttaa
     4681 ctgttcatca gtatatttat catatgcttt tgttgcttgt tttaattctg ctttaacacc
     4741 atcatctttt ttcgatgtct ttgaatcatc cttcttacta tcgtcgttcc cacatgcagc
     4801 aattaataac gttgatgcta ataatagcgt tgttaacttt ttcattttga cacctcatta
     4861 tagaaattct attatcccca tagatgtgtt cttataaata ttattttcgc attccctaca
     4921 cgctaaaata atacattaca cttaaaacgg ctgttttaaa gcatcctccc ataaacatca
     4981 tctagttgat aataattatc aaaatcatta tattgttata aaataatact gtcaacaacc
     5041 ttttattttt aaaaattaaa taaaagagcg ctgccatttt aaatgtcatc actcttttgt
     5101 aagtctaatt tgtcattttt ggtcgtattc tgattttaat aaactataga tgtaactcga
     5161 tgaataaata ccatttagta attcattgtc acgtaacata ccttctctag taaatcccag
     5221 cctttcagga atagcccggc ttttttcatt attaactgcg gcactaatct caattcggtt
     5281 taagtcaagc tcttcgaaac aatactttat caatgcctca actgcttgtg tcataatccc
     5341 atgaccctca aattctttat ctaaatagta ccctaatgaa gtttttctgt gcatgtgatt
     5401 aatttcatgt aaaccgatga caccaactag cgttccttca taccaaatgc cacactgaaa
     5461 tccattacca tcagcaaatt gcaaaagtcc tcttttaata aatgcacgcg tatctgatgg
     5521 ttgctcagtt gcatctaccc aaggtaacca ttccctaagt gaatttcttg aacgattgac
     5581 taaattgaaa agcgcttctg tgtcatgagc ttctaaaatt tttaatgtta tttgttcatt
     5641 cactttcatt ccaaacatac catcacatcc tcattcattt ttcatataat tctgtaaatt
     5701 atcattataa taacatatta atgttacgca ttgtatctta aatttgttgt ccttccccaa
     5761 cttgcattgc ttgtagaatt tcttttcgaa attctctatg ttggggcccc gccaacttgc
     5821 attgcttgta gaatttcttt tcgaaattct ctatgttggg gccccgccaa cttgcattgt
     5881 ctgtagaaat tacggaccca atttctctat gttggggccc atccccaact tgcacattat
     5941 tgtaagctga cttttcgtca gcttctttgt tggggccccg ccaataagcg ttgtagtgcc
     6001 tattactttg attgatgtcc aagtttccta tcacacaact taatttaatc actactttat
     6061 tattcattcc aaatttatat ttaaaaatac gacaaaacag tgctaaaatg ctattaaagg
     6121 aggcactgct catggcatca ttgaaaaata agcattttat gattgggcta tcactaacat
     6181 ttatagtcgc attatttagc tttttagcag ctaaattgcc aatcctagat aaggttggcg
     6241 cattaactat cgcgatatta atcgctattc tatatcgtca ctttagagga tatcctgaac
     6301 aatatagctc aggtattacg ttttcatcta aatatttatt aagatttgca atcatcctat
     6361 atggacttaa actcaatata ttcgatatta tcggtcaagg tagtaaacta ttagccatcg
     6421 atgtaggtgt cgttattttc agtattgtaa tgatgctctt tgtcaacaaa ctgttgcatg
     6481 gtgacaaaaa catcgcatta ttactaggtg tcggcacagg cgtttgtggt gctgctgcta
     6541 ttgccgcagt cgctccaata ttcaaatcac gtgaaaagga tacagctatt agtatcggta
     6601 tcatcgcatt gattggtacg atattttcac ttatatatac agctatctac gctatctttt
     6661 caatgacgac aaatgtttat ggcgcttggt ctggtgttag tcttcatgaa attgcacacg
     6721 ttgtcttagc tggtggcttt ggtggtagtg atgcacttaa aattgcactt cttggtaaac
     6781 ttggtagagt attcttactg attccattaa ccatcgtact tattttaatt atgcgtttcc
     6841 gttcatcaga atcatctagc aagggtcgaa taagcattcc atacttttta attggatttg
     6901 tcattatggc cttagtgaat acatatgtaa ctattccgtc agcattgcta aatattttaa
     6961 atactgtatc aacaatttgc ttattaatgg ccatggttgc tttagggtta aatgttgcat
     7021 ttaaagatct taaaaatcgc gcacttaaac cgcttatgac aatcattatt acctcaatat
     7081 gcttgtcatc acttgccttt atcgtcgttc attggctata tagttaatat gaaaagacgc
     7141 tcaaacactt acaaatttag tgtgccgagc gtcttatttt tatactttaa aagtcgtatt
     7201 ggttgttttc ttcttcaaac acttgttgtt gtactttggc agttcgcatt gtggattgtg
     7261 ccaacgcttt taaccgaaat acaacatcat cattgacaat ttgttgattt gaaaaatctt
     7321 tctcttcaat aaatacatac gtttgcatcg tatgtgcttt catgtaactc aatattggtt
     7381 ttaaatgcat ttcaggaatt aaataatgtt tactagaacc tgctgtcgct acaagtccta
     7441 ttaccttgtc acgaaacgca ttgactggaa gtagatcaaa cacatttttc aaagcaccag
     7501 ggatggaagc ttgaaaaatt ggaaaaccaa taaaaatcac atcagcctgc attaacgacg
     7561 tcgttaattt atatacatct cctgtagtat ctagataatt ctttccaacg ctaaattcta
     7621 attcaagttc tcgtaaatcc atcaactcta tttgatgtcc ctcattgatg acttctagtt
     7681 cattttttaa atcatccata gcaattctcg ttttagaacc tactgtggaa cctgacaata
     7741 atacaatatt catgatgacg cccctcctat tttgataaat gctttttaat cgccggtata
     7801 atgtcgttgc caattaactc aatattcttc ataacatttt caaatggcac accgccaaaa
     7861 tcaagctgtg ccataaaacg ttgatgacca tacaactcgt gttgatacaa tatcttttca
     7921 ataatttgtt gcgggcttcc aaccattagc gcttctcgat aatctgacga attagcaaat
     7981 tgctgtttcg gataaccaac accacgaata aatgacatcc ctgtattcaa atgtggataa
     8041 aattctctca tagcatcctg agttgtttca gctgtataaa acagactcgc tgtacttact
     8101 ggtaaagact taggcgaagc atcgaaacct gcttcagttg ccgcttgacg ataagcatct
     8161 atagaacctt taaagttcat tgctgggcca ccaagggttg taatcatcat tggcacacct
     8221 tgttttcccg ctttaatagc acttgcaggt ggaccaccaa cagcacgcca tattggcaat
     8281 atattatcga ttggtcttgg gaatattttc atatttctaa gttctggacg atattttcca
     8341 gaccaagtaa tacgctcagt tttatttaac tctaaaagta aacctaattt ttcttcaaac
     8401 aattcatcat agtcttttaa atcatagcca aacaagtcaa aaatacctgt tcttgatgct
     8461 ctgccagcta caatttcggc tctaccatga gaaatcaaat ctaatgtcgc gaagtcttca
     8521 aatactctta caggatctgt tgcactaata atcgttgaag aacttgaaac tttaatatgc
     8581 tttgtcgctt gggcaattgc acctaacaca accgtatgtg cctgtgttgt aaaatgctcc
     8641 tgatgacttt caccaactgc aaaaacatca atacctgctt catctgctaa tttacttgct
     8701 tcaataattt cattaatacg ttgctcataa ctaacttttt cacctttcaa tggattcaat
     8761 aaatgatcac ctaaggaata caaaccaaac tcaagaggcg tatttttatt catttctaat
     8821 ttggccatta aacattctcc tttatatttc gtgtaactta ctttcaattt gttcacgttc
     8881 tgcttctaaa aagtcaggca agttcaattg acttcctaat tgttcaacag gtgtatcaat
     8941 agtaaatcca ggcgcttcag tcgcaaattc atacataatt gaattatggc gatagtataa
     9001 tgatttaaag aaatagcgat caattatacc tgaattactt tgtggttgtt gttgtaattt
     9061 cttgtaaatt gcatctaagt cactcatttg tggtgtatta actgcaatat gatggatata
     9121 acctcgtcca ggtctttcac gttgtccctg ttgctcaata actacaaaat ccgaatataa
     9181 accttgtggt gctaatgtca gcacatcgaa tgatttatta tctcttttgc gataacctaa
     9241 gacattttcc aaaaattcta ccgtacgcgc tgcatttcta actcttaatt cgactggccc
     9301 tattcctaaa atttgatatg cttgaggtat ttcactataa gcgttatgtt gccattgatg
     9361 tggtacttca aaactatcat taacaagcaa tatgatttct aagtcgtctt catctttaaa
     9421 gacaacacct tcttgtccaa gatagtacaa acgttcagac ttaataccat tattctcaag
     9481 acgtttttca aaataatgaa gtgccgcttg atttggtact aataatgaaa atcgataaat
     9541 cgtttcagta cctggctgct tatgacccgc attgggaatt tcaaaaaagc ttaaaattgt
     9601 tccggctgta cctacttcgt ccccataaaa caaatgatac attgaaggat tgtcttgatt
     9661 aaccgacttt tcaactaatc gtaatccaag gacatttgtg taaaaatcct tattacgttt
     9721 tgcatctttt gtatacatgg atatgtgatg atgccctact atattcatac tatccctcct
     9781 attagtataa attttatacc agcttacata aaaaagatag ttatatgcca tttacccctt
     9841 gcaaataaaa atatgtttgt actcattcgt agatacacag ctagtatgta taacgacata
     9901 ataattgcat aattataaat ttatgtgtag caatttattt tttctaaatg tttataaaat
     9961 acttcaataa ctaaatatgc aagatttcac aaaagcatat tgacaacgct ttcatacatg
    10021 tctaatataa gtttatcgca caaaatgcat ataaatacat attaaggagg attttatgaa
    10081 ttttcttaaa cctgcaaagc atattaagcc tttgccagaa aatcagatag atgataccta
    10141 taaacgatta cgtctccaag tatttcttgg tattttcatc ggttatgctg ggtactattt
    10201 attacgtaaa aacttttcgt tagcgatgcc ggcattgcaa gagcaaggtt ttacaaaagc
    10261 ggaactaggt tttgcacttt ctgctgtttc catcgcatat ggatttagta agttctttat
    10321 gggtactgta agtgatcgga gcaatgctcg gatattctta gttcttggat tagcactcac
    10381 tgctatcgtc aatttgttaa tgggatttgt accgttcttc acatcaggta tcggtattat
    10441 gtttgtccta ttattcttaa atggatggtt tcaaggtatg ggctggccac cttcaggccg
    10501 tgttctcgtt cactggttta gtgtaagtga acgcggaagt aagactgccc tttggaacgt
    10561 tgcgcataat gttggtggag gtattatggc acctattgct gcttggggta ttacaacaac
    10621 tgcatttatc aactttggtt atttaaaagg tttcgaaggt gtattcattt accctgcact
    10681 cttagcactt atcattgccg caatttcata cgtattgatt agagacacac ctcaatctca
    10741 aggtttacct ccaatcgaaa tttataaaaa tgactttgct acaagcgata agaaaacatt
    10801 agaaacagaa ttaactacaa aagaaatttt atttaaatat gtactgaaca ataaatgggt
    10861 atgggcaatt gcctttgcaa atatatttgt ttatttcgtg cgttatggtg tacttgattg
    10921 ggcgccagtc tacttaagtg aagaaaaaca tttcgactta aaagcatcag gttgggcata
    10981 cttcttatac gaatgggctg gaattcctgg tacattatta tgtggttaca tttctgataa
    11041 attattcaaa ggtcgtcgtg gacctgcagg tttcttcttt atgttaggtg tcacagtatt
    11101 tgtattaatt tattggttaa atcctccagg caatgcttgg ttagacaatg tctcattaat
    11161 tgccattggt ttcttaatat atggaccagt tatgttaatt ggtttacaag cattagatta
    11221 tgtacctaaa aaagcagctg gcacagcagc tggattaaca ggattatttg gttatctgtt
    11281 tggtgctgta atggccaaca tcgtcttagg tgctgtagtt gataaattcg gatgggatgt
    11341 cggttttatt ttattaacag caattagtgt gtttgcaatg ttgagcttta tcctcacttg
    11401 gaataaagta ggacaagaaa ccgttcatca ttaaatgata aaaaataaag tcatatggtt
    11461 atcttatcga aagatgatat attcatctct tataagttca accatatgac tttttattag
    11521 tattcaaaaa aatatttaca ttgccacttt tgtgtttgcc ctgctgtttt attcaattga
    11581 ttacaccact taggataaac tctaaaagcc attttccctt gatacttaga tgaagctaag
    11641 ataccttttt ctaccaataa ttccctagga aatagaaagt agccatttaa ttcatcatcg
    11701 ataacagcaa caatcaggta atcagcaaac tcttcaattt tgtatggccg attataatta
    11761 tcttcgtctt ttgtccaaca tgtcacgaaa tatcccgatt tagtcggtgt tttcttagct
    11821 aacctacttt gatatgtttc ctctttaaag ctaaatgtta atgcttcgta atcttgatta
    11881 tatttttctt cagttaaatc tttaacttct gattcttcac taaaaatatt tttcaacagt
    11941 attttagatt tacacatatg ccaatctcac tttatttttt cataatcgta tcatatattt
    12001 atttttttcg aaaaatacac ttccgaccga aatatactca gccagaagtg gacgaaatat
    12061 ttaaccttct attaattcag atgtatcaac tgtcaaacga tcacgtaata aatagacgat
    12121 taacattgct gctaaagcac aaagtgactc agcaattaat aatgaccaaa tgacacctgt
    12181 tagtccaaac aaagcattca taataaataa tactggaatg ataattgcac cttgtaaaat
    12241 ggccataatt gtagcaccac gaccttgccc agtcgcttga agcataccag taaacaagaa
    12301 acctatacca tttaataata atgatgccat tgttactttc aaaataaatg tcgccatctc
    12361 aacaatggct tgatcagtag taaatagtcc gaccatatga tgtccaattg taaatacagc
    12421 actcatacat acaacaaaga taacgccgat agacatgata actgctttga taacgtcttt
    12481 catacggcct ttatttgcca taaagttata tgcaattagt ggtacaacac cttcacataa
    12541 tcccatgata ataagttctg gaaattgcac aagtctaaat gagataccat aacttgcaat
    12601 cgcgaagttt ccataatgtg ctaaaaataa atttaaaact aatcctgtga atcccattaa
    12661 gatactcatt aaaaatgcag gaataccgat tttaaagatt tcagaaagca tttctttatt
    12721 aggtttcgca agtttaatat taactgacac aacgtcacta tttttcataa aataaatgat
    12781 aaagaacaga gcagcagcaa cattactgat tgcagtaccc aaagctgcac caacaacgtt
    12841 taaatcaaaa ccaaaaatta aaattggatc taaaataata tttaagccta cactagctaa
    12901 cataccaatc atagaaacca ttggtgcccc aattgcacgt gcaaattgtt ctaatatgaa
    12961 gaacaaaatt acaaaaggtg cacttaaaaa cattactttc aaataattac ttgttaaagc
    13021 taacgtttca cctctcgccc ctaaaattgc tgcgatttga tcactgaatg gtaaagtaac
    13081 taaaatcacg ataagtccta gtgcaatacc accataaata gagaaactac ttacaaattt
    13141 actcttacta tagtctttcg cacctaataa acgtgaaata taagttcctg caccaacgcc
    13201 aaataaatta cctaacccca ttaagatagc aaatactggc agtgttagag agatagcaga
    13261 aatcatgtgg ctatcttcta aaaatcctat aaagtaaata tttaatatgc cataaataac
    13321 gcttaataaa gtccctatca tcattggcaa tgagaaatgc atcatcgctt taaatactgg
    13381 cgatttctca aaataatata attgttcgtc tttcattgtt caatactcct tgtcttttcc
    13441 aataattagc ttactaacaa ttagatatct aactataata ttaaagacaa agtgactgat
    13501 ttctaccagt cacacttatc atttattgta aactagataa cattttagtt aagtttgctt
    13561 tcatttgttc attttcttct tcagataact gcgatacgag tgtttgttcc atttcatcaa
    13621 atatcgaagt gaatgcttct acgagtttaa tcccagaggt agtcagccct atattctttc
    13681 ttctcgtatc ttgtgcatcg acatagcgat agatcagctt tttacgttca aggttcctta
    13741 ataaattact gacagttgga cctgttcgtt gtaatgcttt agcaatatca ttttgtgtca
    13801 gtccatcttg ttgatgtgca taaagataac ctaacgtatg accttgctca tttgtaatat
    13861 caaattgctc taacttttga tcagcctttt gtttcatctc atgactaatc attctaaata
    13921 aatacgaata agtgaattcc atttattgca ccaccatttc gttagatgtc taactaaata
    13981 tactgcgaca tttttaaatt gacaacatga atatctaatt tattttaaaa gttcctataa
    14041 aatcttgcgc cataaacatt ttgatatgca atttttcgga ttaaaatatg aaagagattg
    14101 catatctacg tttttaagaa atcgcttaca attacaaata ggaggatatt acgttgttaa
    14161 gttatagaca aatcgaaatt ttgtatcttt taattcatga gcaaacgttt attccaatca
    14221 ataccattgc taatcaactt ggtgtatcac ctcgtaccat tcaatatgat atagcatata
    14281 ttgagcaata tgctgaaact tatcattacc aagtcagccg taataaagct gcaggcatca
    14341 aagtaacaac agctcacgcg acactattaa atgaattaga gcacaatcta acaaatcaaa
    14401 ttcatttttc aaaagatgaa cgactcacac atatcgcttt aaagttattc gaaacaaccg
    14461 atcctgtttc aacaaagcaa cttgcgcaag atgttaatgt ttcgcgtcgg acaattgcag
    14521 atgatattaa aatgattcaa gcccaactag atcaatacca tttaaagtta aattatgtcc
    14581 acaataaagg attcaatatt attggtgagg aagatcatta tcgtaaagcg tatgcacact
    14641 ttatacatca atatatgaaa caagctgcac cttttataga ggcggatatc tttaattcag
    14701 aatcaatcgc attggttcgc cgtgccatta ttaagacatt aaatagtgaa aattatcatt
    14761 tagttcagtc ggctatcgat ggcttaatct atcatatact cattgccatt cagcgtttaa
    14821 atgaaaattt ttcgttcgat atacctatca atgaaattga taaatggcga catactaatc
    14881 agtatgctat tgcttcaaaa atgatagaaa acttagaacg cagttgtaat gtcacatttc
    14941 cagagtctga aattatcttt atcacgttac atttactagg ctctaaaatg actgaacata
    15001 cagcatcttc aattaccttt gaataccatg atttatcgca aaatatacat gaattgatca
    15061 cttgtgttag ccaagaatta ggcattgata tgtcaaaaga caacaagtta cataccagtc
    15121 tgatcacaca tatcaaacca gctatacatc gtattaaata cgatatgcta caacctaatc
    15181 ctttgaggca agaagttatg cgtcgctatc ctcaaatcat tgaagccgtt agcaagcata
    15241 ttagtccaat tgaacaagat gctgctattc gcttcaacga agatgaatta acatacatta
    15301 caattcactt cgcatcaagt atagagcgtg ttgcaacaca taaacaatca atgattaagg
    15361 ttgtcttact atgtggttct ggtataggca cgtcacaact tttaaaatca aaactaaatc
    15421 acctgtatcc tgagtttcac atttgggatg cctattccat ttatcaattg gaagaaagtc
    15481 gattattgca agataacatt gattatgtca tttcaacagt accttgtgaa atatcagctg
    15541 taccagttat tcatgtcgat ccatttatca atcaacaatc tcgtcaaaaa ttgaatcaaa
    15601 ttatcaatga ctcaagagaa caacgagtca tgaaaatggc aactgatggc aagtcactcg
    15661 cagatttatt gcctgaacat cgcatcatta taaataaaca accattatca attgaatccg
    15721 caattgcagt ggctgtgcaa cctttaatca atgatggcat tgtctattca aattatacag
    15781 ctgcaatttt aaaacaattt gaacaattcg ggtcatatat ggtcattagt ccacatattg
    15841 cacttattca cgctggtact gattatgtac agaatggtgt aggtttcgca ctaacatatt
    15901 tcactgaagg gattatcttt ggtagtaaag ctaacgatcc cgttcacctt gtaattacat
    15961 tagcaacgga ccaccccaat gcacatttaa aggcattggg acagttaagc gaatgcttaa
    16021 gcaacgactt atatcgacaa gatttcttag atgggaatat ttttaaaatt aaacaacaca
    16081 ttgctttaac tatgacaaag gaggcttaat aacgtgtcat tagacatttt gtcaacaaca
    16141 cgcatcattg taaaagaaca agtaaatgat tggactgaag ctataactat agcttctcag
    16201 ccattactac aagaacaaat tattgaacaa ggctatgttc aagcaatgat tgatagcgtt
    16261 aatgaacttg gaccttatat cgttatcgca cctgaaattg caattgcaca tgcaagaccg
    16321 aacaatgacg tacatcaagt tggtttaagt ctattaaagt tgaatcaaca tgtggcattt
    16381 tgtgatgaag atcactacgc atctctcatt tttgtattga gtgccatcga caatcattca
    16441 cacttatctg tattacaaaa tttagcaacc gtactgggcg ataaccaaac agtccagcaa
    16501 ctattaactg caacaaatgc acaagacatt aaaaacattt taaaggagca tgattaatat
    16561 gaaaatttta gtagtatgtg gccacggttt aggaagtagt tttatggtag aaatgaacgc
    16621 acaagaagca cttaggcaac ttaatgcacc atctgatatc gaagttgaac atagtgacat
    16681 tatgacagca agtccagaga tggctgactt gtttatttgt ggtagagatt tagctgaaaa
    16741 tgccgaacgt ctaggggatg tcttagttct tgataatatt ttagataaag ctgaattaca
    16801 acaaaagctc tcagaaaaat tacaacaact taacatgatt taaaggaggt acgacctatg
    16861 caagcaatcc ttaattttat agtcgatatt ttaagtcaac cagccattct tgttgcactg
    16921 attgccttta taggtttaat cgttcagaaa aaacctgccg caacgatcac ttcaggaacc
    16981 attaaaacga tattaggctt cttaatttta agtgcaggtg ctgatgtcgt cgttcgatct
    17041 cttgaaccat tcggcaaaat attccaacac gcatttggtg tgcaaggtat cgtacctaac
    17101 aacgaagcta tcgtctcact agccttaaaa gattttggaa caacagctgc actcatcatg
    17161 gtctgtggca tgattgttaa tattttaatt gcccgcttca ctaatttaaa atatatcttt
    17221 ttaacaggtc atcatacatt ttacatggct gcgtttttag caatcatttt aacagtcagt
    17281 catattaaag gctggctaac gattgttatc ggcgcactcg tattaggatt aatcatggca
    17341 gtattacctg cattactcca acctacgatg cgaaaaatta cagggaatga ccaagtagct
    17401 ttaggtcatt ttggctcaat cagttacttt gccgcaggtg ctgtaggtca attattcaaa
    17461 ggtaagtcta aatcaacgga agagattaaa tttccaaaag gcttaagttt cttacgagaa
    17521 agtacaatta gtatctcgat tacgatggca ttactttact tcatcgcatg cttatttgcg
    17581 ggcgttagtt atgtacacga atctattagt gatggtcaaa actttattgt cttttcatta
    17641 attcaaggtg tgacatttgc tgctggtgta tttattattt taacgggcgt tcgtttaatc
    17701 ttagctgaaa tcgtcccagc atttaaagga atttctgaaa agcttgtacc aaattctaaa
    17761 cctgcattag actgccctat tgtgttccct tatgcacaaa atgcagtatt aattggattc
    17821 tttgtcagct ttattacagg tgtcatcggt atgtttatct tattcttatt tggtggcgtc
    17881 gtcattttac ctggcgtagt tgcacacttc ttcttaggtg caacggctgc tgtattcggt
    17941 aatgcaagag gcggtattaa aggtgctatt gctggcgccg ctctaaatgg tatcctaatc
    18001 acgtttttac cattattatt cttgccattt ttaggcgaat taggtggtgc tgcaacaaca
    18061 ttctcagata cagacttttt agctgtcggt atcgtgttcg gtaacgcagt aaaatatatg
    18121 ggattatttg gtgcgattct atttattatt atcgtaggtg cgacaacaat tttattaaaa
    18181 ggccgtcaaa aagaacagca atagtgttaa cgtagaaata taaaacaccg tcacatattg
    18241 agtgaatgcc ccttttatca agaggaaagc cacttactta tggacggtgt tttgtattat
    18301 attaaatgat acttagccat actatcgaca gctgctaaaa ttgcttcttc ttgtgtcgca
    18361 atcggttccc aaccaagtaa tgtttttgca cgttcgttac ttacattgcg gttcatatct
    18421 aataaaagtt taccttcttt agcttgatga ttaaatttag cacctagact caaaataaag
    18481 tctggtaatt ttttagtaga aactttttga gctatttcag gtctcttttc tttaattaat
    18541 tttgcaattt ccaacaaatt aatttgtcca tcagccgtcg caataaatcg cttgccatta
    18601 gcttgttcat ttgtcattgc caaaatgtgc agttcagcta cgtctctcac atcaacaaca
    18661 tttaacggaa tttgcggtac acgtttcatt gaaccattca ataaattttc taataaatga
    18721 aagcttcctg aaacgtgtgc atctaatgat ggcccaaaaa ttgcaactgg attgattgtg
    18781 gcaaattcta ctgttgtatt ttcattctca acaaaatccc acgctgcctt ttcagctaac
    18841 aattttgatt tttcatatac tgataagcct ggttcatctt catttgtcca atgactttca
    18901 tttgtgattg aatttttatc tttattacta aaaccaactg caccaaagtt tgcagtcatt
    18961 accacacgtt taacacccgc atgttctgca gctcttaaaa tacgttgtat accttcaatt
    19021 gcaggcttcg ccatcacttc tgcatcgtct gttttaccga aaaacaccgg agatgctaca
    19081 ctcaagacat acttgcaatc tttcattgct tcatcccaat gttcatcttg tgataaatcc
    19141 gcttcgacaa acattaatcg ctctgtggaa atgccattgt cttgcattgt tttaattact
    19201 ttatcagctt tacttaaatc acgtatcgtc gtttgtacgt cataaccttg ttctaataat
    19261 cgtgaaataa ttcgcatgcc aacaaaccct gtaccaccgg ttgctaatac tttattattc
    19321 atcacataat ctcctctttg tgtctattga ttactcatta tagcagtcat tatattttgt
    19381 atatatcaac agacttatat tgcttaatat tctatatgaa ttataaaagt aaacatcatt
    19441 atattcctta aaatattaaa ctcatctatt ttcaaatacc caatcattta gaaacaaaaa
    19501 ccatatatat atcatacttt gaatagttaa aggcaataat ttccactcat atgttcaacc
    19561 ttcaccaaac actattgtca ccttttcaag agcaagcaaa cacttccaat gattcagcat
    19621 acataaaata acaatattaa cttaaaatca tatccactaa tgcttctacc gtaacattgc
    19681 taaaataata aacgatgtct aattgcttta actgatgcgt taaatcttct cttgtcattt
    19741 ttgttccttg taatgcttct tccacatctt ttatatcacc ttgtccaaag aaatccccat
    19801 aaatacgaca atctgcgata cgattttgtt caacagaaat tgttatgtct accgtacctg
    19861 aagataatct ttcacttcga ttgtattcat atttgggtga cttgccataa ttccaatccc
    19921 aatttttata ctttttatca gctaattcat caatcgcttc ccaatctgca tccgttaatt
    19981 catatcgttt cgcctcttta atgtcatcga ttcccaaaat ctgcgtcacc attaaatttt
    20041 taaattcttc aatggttata tcacgatact tttcatccag tgcttctcta agatgaccaa
    20101 cacgtgcgcg cacagattta atccctttcg atgcaatctt tttgcgatta ggctttaaca
    20161 ctttatccat tgcttcataa ttaacatcaa gtaatagcga ataaccgcca taaatacgat
    20221 tattcattaa tgtcattgcg gcgcctgaca cttttttacc attcaatgtt aaatcatttc
    20281 taccgctttg taccacatct gttgcaccta atgtatgcaa cgcctttata gctggttgat
    20341 aaaatcgttg aaaatcacca taaattgaag tgtcttgttc taaaatacaa cacatattaa
    20401 cggcaccttt atcaacatac acagcgccac caccagtatc tcggcgaact acttgaatac
    20461 tgtgtttatc tatataatct tgattcactt ctatagcagt attttgaaaa cgtccaattt
    20521 ccacttttgg atcacaataa taagggaata aaatatcttc atctaaaaag atatgctggt
    20581 taacataaac ttgcatagcg agtgcaatcg caccatcagt aatatattct ccatttctaa
    20641 tcggttctat taagtacatc ttctgtctct ccaatgttgt tatttttcgt tgtgctgtcg
    20701 ttccggagtg ctgctgtaat caatgttgag atatcctcag ttaaatgtat ggtacgttct
    20761 tgaattgaat tcggaatgcg atatgccttt ttattcatcg tcatataaag ggcattttca
    20821 tttttacgtg tcatacgctg aaaaggatgc ttcacaaatt gtggtgtagt ataaccaatt
    20881 ccaatttcca aatacaacac tttatcatct tgatgttgct ctagaaaagc attataacga
    20941 tgtagttgcg catgaaattc agcatcttca accatcccaa cttccgcttt acgtttattc
    21001 acttccattg gggcatcaca ttttggacat cttggaatca tctcccaagg tataagcata
    21061 tcttgttgcg caacaaccat tttacgaatt aaatcatcat tgcgatacgt ttgagcatga
    21121 caatgctgac tacactgttg cagtatatac tccccttgta tatgaaatac atgagtcata
    21181 tcataatcag ctacatcgaa agcattatct gcattcgtag ttataatgtg gtactgttta
    21241 ccttccacca aggattttaa agcgaggtaa gactgaccta caggttgatc taaatagttt
    21301 aatgtaataa aacgactctc aaatgcccaa tactcttgcc aactgccata aggatgtaaa
    21361 ctcgcttgca acatatcaaa gaagcgatat ttttcaataa aatctgggaa attttccgta
    21421 aaacgctctc ctacatatgt aaatccgtca gatgcagaca tgcctgcacc aattccaatc
    21481 actatcgcat ctgcttcatc aatcgcagta cgcaatactt cagcctgctt tgtcttgtca
    21541 tccattaaca gagacattgc attccactta ctactctgca tcacggttaa atgcctcctt
    21601 atacagttgt aaatccttat ctgtaaatac attgaacacg actttcaatg ttgaatttgt
    21661 ttctttgaga tagctttcta ctgttcgaac agcaatttct gctgcttcat cttgaggaaa
    21721 agcaaataca cctgtagata tacagcaaaa agcgacatga tttaaactat gttgatcagc
    21781 caatttaaga cagctaagat aacatttagc taacaagtcc tgattcatct ttgaaacagg
    21841 caatcgacgt atttgcggac caaccgtatg aattatatac tttgctgaca aattatatcc
    21901 acgtgttatt ttggctttac ctacaccttc attgcgccct tgttgtcgaa tgatctctgc
    21961 acaatcaagt cgaacttgaa cacccgcttt tgtatgaata atattatcaa tgcagtcatg
    22021 attagcttgc atacatccta gaaaacgact atttgcagca ttaacaatag catcgatttt
    22081 taacgtcgtg atatcacctt gccaaacaaa gatattgtca ccttttatcg gcttcaaatc
    22141 cttcaaatcc gtaacatgtt gacgattcaa atcacttaac atagcatctt gtacagctaa
    22201 atattcatca cttacaggta atgccggtct gacatttgct aagcctcgat ataattccca
    22261 caaatcttca aatgaagatg gcattaccaa tacgtcattg tcatttctct ctcgatgcat
    22321 atcattgatt aaatattcaa gtctcgcttt atttgatttt aacgtttcca tttaagcctc
    22381 cggtaatgct aggaatgctt ctttatcgac atcatccaat ttgaacaacc aattttcttc
    22441 tggtttttca gagtttaaaa ttgtcggttc ttcttccgct tttgtatttc gctcaataat
    22501 cgttcctgac aatggcgttt gcacatcaat gaccgttttc gatgcttcga tactcacaat
    22561 ttcatcatcc actttaactt catctggact tacgaattca acataaccta ctgtcccaat
    22621 atcatcttgc aattcaggtg tcatactaaa cacatacaaa tctcctactt tttctaccca
    22681 taaataattg gctaactttt tcatcttatc ttcatccttt ctcttctatt taaaatgcta
    22741 caaataaatt tccgcgagta attttaatgt tttacaacgc tgttcaatac ctggaataag
    22801 cggtgctact aacacctcat caacttcaaa cgtagcaatg aaatcatcta attgtgcttt
    22861 aaccttttct tgtgtacctg caatgatgcg tgcttgatgt gcttgaatca tctctttgtc
    22921 tcgatcatta agcttatact tttgtgctgt gtctactgaa ggaaaatctt caaattctgc
    22981 aaattgtaat ttacctaata accaaacatc taaggcatgt tgtaatgctg ctacttccgc
    23041 ttcgttatca gctacaatga caaatacaga tgccatcacc tttgcgtcca ttttaatcgt
    23101 tgatgcttgg aaatgttttt tgtaaatatc aatgttatcc ttcgctgcat gtatcgcatt
    23161 tatatctggt agcaaaaatg ttccaacaga aagccctata cctagttcgg cagctatttt
    23221 ggcagatgtt gcgctactac ttaataacca catttctgga aaatgatcaa tgtgtggttg
    23281 gacacctaac gtatgcgcac ttggtttatc ctttattgta agataatcac gtaataacga
    23341 aatcgattca tcgtaactat catatgtagg atttattcca tctaaagctt gctttaccat
    23401 agtagtacct ggattattac caatacctaa atcaatacga tttggatata acgctgccat
    23461 cattctaaaa tgctcagcaa ttttataagg tcgatagtgc ggcagcatca caccaccaga
    23521 gccaactcgt atgtgatttg tctgcgccaa tgtatgcatc atcaaaagtt ctggactact
    23581 acacgcaaac gctggtacat tatgatgttc cgtaaaccaa attcgcttaa agccaagtcg
    23641 atctgctaat tttgcaagtg tcactgaatc ttgcaatgcc ttttgtgcat ccttaccttc
    23701 atctattaag gcatagtcta atacgcttaa tttaaccaat ccgtcatctc caaacttatc
    23761 ctgtcatgtc aaaccgacat aacattttag cgttcttaat accattccct cttcatatac
    23821 ccacgtatat gataacgttt ttcaattaac ttttatatct tttcgccttt atttttcttt
    23881 ttcatattca tattttcaga cctacaaatg ccattcatta gtttaatttt aaaaatactt
    23941 tccatgtacc aattctattc tatcaagact gttcatcatt gattattcat tctttccatg
    24001 gtactttgaa aacaatattg aatagaaaag gaatcatagt atgtaccgat acaaaccact
    24061 tttacaatca atccacttac ctaatggcat caaaatttcg aatcgctttg tattatcacc
    24121 aatgaccgta aacacatcaa caaaagaagg ctatattaca aaagaagact tggcttatgc
    24181 tgcacgtcgc tctaattcag ctggaatgca agtcaccggc gctgcttaca ttgaacctta
    24241 tggtcaattg tttgaatatg gctttaatat tgatcatgat gcttgtatcc ctggattaac
    24301 taacatggca tctacgatga aacaacatgg tagcctcgct attatccagc tagcgcatgc
    24361 tggtcgtttt tcaaatcaag ccattttaaa ttttggaaag gtgtatggac caagccctat
    24421 gacattgcat tcaccgattg aacacgatgt tatcgccatg tctcatgaga aaatcaatag
    24481 catcattcag cagtatcgcg atgctacatt acgcgcgatt aaagcaggtt ttgatggtgt
    24541 ggaaatttcg attgcacaac gtttgctgat tcaaacattt ttctcaactt tttcgaataa
    24601 acgtacagac cattacggtg ccgactcatt aaaaaatcgt gcaagattat gtttagaagt
    24661 tatgcgtgcg gtacaagaag tgattgataa agaagcacct gacaatttta ttttaggatt
    24721 tcgagcaacg ccagaagaaa ccagaggcag tgatttagga tataccattg acgagttcaa
    24781 ccaattaatc aattgggtca tggacgtttc aaacatccaa tacttagcaa tcgctagctg
    24841 gggtcgacat atttatcaaa atacgtctcg tacaccaggt gatcatttcg gtcgaccagt
    24901 taaccaaatt gtctatgaac atttagcagg tcgtatccct ttaattgcaa gtggcgggat
    24961 taattctcca gagtctgctt tagatgcttt acaacatgca gatatggtcg gcatgtcttc
    25021 gccttttgtc acagaacctg actttgtgca taaacttgct gaacagcgac cacatgatat
    25081 caatcttgaa ttttctatgg cagatttaga agatttagcc attccacatg ctgcttttaa
    25141 agatattgtt aaaatgatgg attatgggga aggtctaaag aagcataccc gtgacgcttt
    25201 acgtcaattg gaacagaatt atcatgactc atcgtcttaa gactattttt catggaaatt
    25261 taagacagtg ttataatgac atcaatatca aaaataagga gcctattaaa tcgtgactga
    25321 aattaaagat aaagtaatta ctaaagatgc atttgcatta ccttacacaa ttattaaagc
    25381 aaaaaatcaa ccaacaaaag gcgctattgt ctacatccac ggtggtggat taatgttcgg
    25441 taaagctaac gatttatccc cacagtatat cgatatcctt acggaacatt acgatttaat
    25501 tcaactaagt tatcgtttat taccagaagt aagtctcgat tgcattattg aggatgtcta
    25561 cgcatcattc gatgcgattc aatcacaata ttctaattgc cctattttta cctttggcag
    25621 atcatcaggt gcatatttaa gtctattaat tgcacgagac agagatattg atggtgttat
    25681 tgatttttat ggttatagtc gcatcaacac tgaacctttt aaaacaacta atagttatta
    25741 cgcaaaaata gctcaaagcg taaacgaaac aatgattgca caactcactt caccaacacc
    25801 ggttgttcaa gatcaaatcg cacaacgttt tttaatctac gtttatgcac gtggcacagg
    25861 taaatggata aatatgatta acattgctga ttatactaat tcaaaatata atattgcacc
    25921 agatgaactt aaaacacttc cacctgtttt cattgcgcat tgtaatggcg attatgatgt
    25981 tcccgttgag gaaagcgaac atatcatgaa tcatgttcca cattcaacat ttgaacgcgt
    26041 gaataaaaac gagcatgatt ttgatcgtag acctaatgac gaagcaatca ctatttatcg
    26101 taaagttgtt gatttcttaa acgctataac aatgatgtaa tatatcatta ttttaaaaaa
    26161 ctcaatttat catcatgata aacagttatt cgatcaacca tattattcat acaatttatt
    26221 aagctcataa acagatacgc ctgtcccttt agttttcgct aatgagacag gcgctttatt
    26281 tcactttact atttttaaat tcaacactgt gatgctactt atatctgatg tgccgtgata
    26341 acagcacgat ttacatagca tattaaattc agaagatgaa tttaacttgc tttcaattgt
    26401 gttcctttac tttcagtcgc ttcaacacgc aacaagtcat ttataatacc tgtatagaag
    26461 ttggcaagtt ctgcaccttt acgtttgaaa tccaggaagt ccacaccgat aaaatcgaca
    26521 tgatcccatc cttgtatgat tggtttaact tgccagatac ctctgcgtgt ggcaggttca
    26581 tcattcgtaa cattaacaaa tggttgattg gacggatgta atgacgaaat cactggtacg
    26641 acaccatcat ttttacgcca ttcttctctt gcatcatgac caataattct actcgttgta
    26701 gccattaaga aaaatgtacc taaatcagga ttttcataac ctaatggacc agtatgagat
    26761 gatacacctg tataagtcgt atacgtaata ttaggattca tacttgtcat gttgttcaat
    26821 tttgcagagc catctaacgt taaatcatag gcagcattgt cgtctgatgt ccaaattttg
    26881 cttttactaa cgcgttttat atagtcaatg taactctcat ttggtaattg tttaaagccc
    26941 cattgcgtta atcctaaatc gatattcgaa tacttgttac ccataaatcg atttaaagcg
    27001 aacatgattt ttctaacagc ttctgtattt ccaaacttat cagctgcttg tgaaccatta
    27061 tgtggtgttg ctaatgttgt gattgatgca accatattgt tatgaccacc agtgaataat
    27121 ggtgatattt ctccaccatg cgctttatga taggcaattt cttctttgtt accatttctt
    27181 aaaaactctt ccattaaacg aattgtttga ccacccatac tatgccctac aagatgtacc
    27241 tttttaccag gttcccaatt aggcatgatt cctttataag tcttaccata gcgctcatgt
    27301 ccgtatttag ctgcatgtgc tgcgccataa tctacgcgac cacctttaat gtaataataa
    27361 agttctacag cgcgatcata gttactacca aatgcactta cacttgcttg atgtacatta
    27421 tagccttgct ttctcaattc ttcgataact ttaaatttat ttccacccca ataatttgga
    27481 tataaagcag gtgcattatc gcctactaat cctaaaaatc catgtacaaa aacaactgga
    27541 tatttgttaa gtggttgtac ttgattcgct ttaagtggtc gaaccttttt agcatctgct
    27601 gctgcatttg tagtcgaacg tgttttaggc gcttgaagcg catctttgat tagttgttta
    27661 ttatcagtat ttttatctgt cggtttctct ggggaatttt gaccatcgat atgtttactt
    27721 aaatcgccaa cttgtggttt ctgttcactt tggcgaacag tatcatcttg ctttgcatct
    27781 atgatttgat gcgcaggttg atgtgtatct tgttgatttg gatgtttgtc cgtggttgca
    27841 tcttgtgctt ttgttgattt aggtgcagtt ttatcattag acgggggtgt agttgaagtt
    27901 ggtgctgctt tatcatgatt gggtattacc ttttgtgccg gaatggatgg ttgtgattta
    27961 tcaacatcat tcgattgtga cgttgtcgat tgtgatccat tacgttcagc atgatgggta
    28021 ttttgaacct gatcattttg acgttcagta ttattagtcg cattattttg gtgtgactgt
    28081 ttagaacctt gtcgttcaga atcttgcttt acttgattat cattttgcat tctgattgtt
    28141 ttactatttt gtgttgattg atgcttcgat gattgaagcg tatctttacc ttctgtcact
    28201 gttccacttt taccattctc tttatgcata tcgtctaatt gctttcctga ctgcatttga
    28261 tgtgacgtta tcgcattccc ttgttctccc ggttgattta gtgtttcttt ttgtgccgct
    28321 gcattagttg atgttttttc cgaggcttgt gcttcatgtg atgacacaac aaacattgta
    28381 gccgctaaca ctgacaccac gcctattgaa tactttctaa tactatactt tctttcttct
    28441 tgtcctctta acatataatc acctcaaaag tatttttttg accccctctt gagaatgaaa
    28501 aacaactgca ccttcaatat aacatgacat atattatttt ttgattaaga ctacaaaatt
    28561 aataaaaatt taataatacg atattaaatc gatactaatc atttacaaat atgtgtattt
    28621 ccaaataatt tataaacact tttagttgtg gtgttgaatc
//
LOCUS       DAKTGS010000018        27375 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.018, whole genome shotgun sequence.
ACCESSION   DAKTGS010000018 DAKTGS010000000
VERSION     DAKTGS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27375
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.018"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(15..482)
                     /locus_tag="O0286_000669"
     CDS             complement(15..482)
                     /locus_tag="O0286_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0169066.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(668..2218)
                     /gene="adcA"
                     /locus_tag="O0286_000670"
     CDS             complement(668..2218)
                     /gene="adcA"
                     /locus_tag="O0286_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCY0169067.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(2407..2478)
                     /locus_tag="O0286_000671"
                     /pseudo
     CDS             complement(2407..2478)
                     /locus_tag="O0286_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2513..2779)
                     /locus_tag="O0286_000672"
     CDS             complement(2513..2779)
                     /locus_tag="O0286_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0169068.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(2779..3030)
                     /locus_tag="O0286_000673"
     CDS             complement(2779..3030)
                     /locus_tag="O0286_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0169069.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(3195..3296)
                     /locus_tag="O0286_000674"
     CDS             complement(3195..3296)
                     /locus_tag="O0286_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169070.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(3303..3902)
                     /locus_tag="O0286_000675"
     CDS             complement(3303..3902)
                     /locus_tag="O0286_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCY0169071.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(3921..4280)
                     /locus_tag="O0286_000676"
     CDS             complement(3921..4280)
                     /locus_tag="O0286_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0169072.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            4535..5785
                     /gene="fmhA"
                     /locus_tag="O0286_000677"
     CDS             4535..5785
                     /gene="fmhA"
                     /locus_tag="O0286_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCY0169073.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(5882..6613)
                     /locus_tag="O0286_000678"
     CDS             complement(5882..6613)
                     /locus_tag="O0286_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0169074.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(6610..7329)
                     /locus_tag="O0286_000679"
     CDS             complement(6610..7329)
                     /locus_tag="O0286_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCY0169075.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(7310..8089)
                     /locus_tag="O0286_000680"
     CDS             complement(7310..8089)
                     /locus_tag="O0286_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCY0169076.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(8210..9649)
                     /gene="mdeA"
                     /locus_tag="O0286_000681"
     CDS             complement(8210..9649)
                     /gene="mdeA"
                     /locus_tag="O0286_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCY0169077.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            9823..9927
                     /locus_tag="O0286_000682"
     CDS             9823..9927
                     /locus_tag="O0286_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCY0169078.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(10081..10767)
                     /locus_tag="O0286_000683"
     CDS             complement(10081..10767)
                     /locus_tag="O0286_000683"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCY0169079.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(11094..11960)
                     /locus_tag="O0286_000684"
     CDS             complement(11094..11960)
                     /locus_tag="O0286_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0169080.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(12513..12617)
                     /locus_tag="O0286_000685"
     CDS             complement(12513..12617)
                     /locus_tag="O0286_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169081.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            12712..14022
                     /gene="sbi"
                     /locus_tag="O0286_000686"
     CDS             12712..14022
                     /gene="sbi"
                     /locus_tag="O0286_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCY0169082.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            14558..15487
                     /gene="hlgA"
                     /locus_tag="O0286_000687"
     CDS             14558..15487
                     /gene="hlgA"
                     /locus_tag="O0286_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCY0169083.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            16054..17001
                     /gene="hlgC"
                     /locus_tag="O0286_000688"
     CDS             16054..17001
                     /gene="hlgC"
                     /locus_tag="O0286_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCY0169084.1"
                     /translation="MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            17003..17980
                     /gene="hlgB"
                     /locus_tag="O0286_000689"
     CDS             17003..17980
                     /gene="hlgB"
                     /locus_tag="O0286_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCY0169085.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(18032..18499)
                     /locus_tag="O0286_000690"
     CDS             complement(18032..18499)
                     /locus_tag="O0286_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCY0169086.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(18510..19202)
                     /locus_tag="O0286_000691"
     CDS             complement(18510..19202)
                     /locus_tag="O0286_000691"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCY0169087.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(19213..20328)
                     /locus_tag="O0286_000692"
     CDS             complement(19213..20328)
                     /locus_tag="O0286_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCY0169088.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(20306..21313)
                     /gene="bioB"
                     /locus_tag="O0286_000693"
     CDS             complement(20306..21313)
                     /gene="bioB"
                     /locus_tag="O0286_000693"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCY0169089.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            complement(21315..22673)
                     /gene="bioA"
                     /locus_tag="O0286_000694"
     CDS             complement(21315..22673)
                     /gene="bioA"
                     /locus_tag="O0286_000694"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCY0169090.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(22651..23337)
                     /gene="bioD"
                     /locus_tag="O0286_000695"
     CDS             complement(22651..23337)
                     /gene="bioD"
                     /locus_tag="O0286_000695"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCY0169091.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(23391..23558)
                     /locus_tag="O0286_000696"
     CDS             complement(23391..23558)
                     /locus_tag="O0286_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169092.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(23789..25522)
                     /locus_tag="O0286_000697"
     CDS             complement(23789..25522)
                     /locus_tag="O0286_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169093.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(25547..27310)
                     /locus_tag="O0286_000698"
     CDS             complement(25547..27310)
                     /locus_tag="O0286_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169094.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         9286 a         4939 c         3723 g         9427 t
ORIGIN      
        1 tattttcaac aactttattt caataaagca tcaagttgat ctaaagtttg attcatacct
       61 tgttcaacac ccatgtttat gacttgttga gcggcttctt ttgttggaaa taccgatgtt
      121 gatgtcactg tcgttttcgt tgtattgctc ttggaaaatg acaaagtaat tttcatgctt
      181 ggcatttttg tatcttttgc accttgaggt gtcgcaaatg agtcaatata ttcaattaaa
      241 tatggacgct taaccgtttt atattctgct aatgtatagc tggtcattgt aggtgtttta
      301 atcgcataaa atgcatcacc gcctgaaaca gcattaaagc gaaacacttt ggtactagcg
      361 tcttttggat gaaaccattt ttcaaataat gcttttgtcg tataggcatc aaacaccttt
      421 tcaatcggtg cttctatcgt tcttgaaaaa ataattttat tatcttcaac tttaatcgtc
      481 atcttcccac tccctatctt ttatatttac tcatcttaac atgcattgag taacatatgt
      541 taccaaatca tttgttataa actatagtaa tattaatcat tccctctgtt gaaattgata
      601 actacgaaat aaaaaacaca ctctattcag ctattaacag ttgagtagag tgtgcaaacc
      661 tgttgaatta atgcgctaac atttcttctt tgatttggtc tttatttaat tttgaaggat
      721 aatatgttgg ccagttatcc atttctttta ataacgcgtc attatcatta cccataaaga
      781 tatggaagtg ttctgccttt ttaggtgcaa tgttgtgatc gctaaattgg atgaatttcg
      841 gtaagtcttt atcattacca tcgaccaatt taaacataaa tctcacgcca cggttacctt
      901 taggatattt caatgttttc ttaccattgt attcatattt accagtgtgt ttcttaccat
      961 ctttagtaaa tgtaatttca tttcctttta tatcaatgtt agtaatatca gttttatatc
     1021 ctttgtcata ataagctttt aaatctttag cagatttctt cggatcattt tcagctttat
     1081 gttccatcac ttcatcaagc gtaccgtctt ttaagtaagg gtaaacagat tgccattcac
     1141 cagcataatc gcttaattca cggtctttaa cttgctcatc tttaaaatac ccatcagaaa
     1201 ttgctttgtc atgtttactt tcagctttgt cgtctttcac tttaacacca ctgtctaaag
     1261 ctttaccgat attttcaata ttagatttca ttaatgattg atacgtaata ttatcttttt
     1321 tctgttgttc tttatttaaa gactccatgt tgtagaattt taaaggcttc gcatctgttt
     1381 ctttacgaat tgtttcagtc actttattcg caacattatc ttcgtaaaga atatatttag
     1441 cattgctatc tctaatttct ttaacaattt tagttaattc tttttgtgat ggatcttcag
     1501 cattcatgtt ttgaatacct ttttgaacaa aaccataaca atcagctaag tatccaattg
     1561 attcatgtga aatgaatact gcattacctt gcttatcttt tgtaacttgc ttcatatcgt
     1621 tatcaatttt cttaagatcg tcgtttaatt ttttgtagtt tttctcatag tcatctttat
     1681 gttttggatc ttttttcact aattcatctt taatttcttt agcgaaagtt tggttaattt
     1741 taggatctaa ccatacgtgt ggatcatatc caccatgatg atgatgatgt tcttctttct
     1801 catgatcatg tccctcatgt tcatgctctt caccatgctc gtgttgatca gttaaaagct
     1861 ttgctttatc taatttatcc tctaaagaca gttttttatc tttatctttg atagtagatg
     1921 caactttctt agcaaccgga tctaaattat cccctgtata cataaacaag tctgacttgc
     1981 ttgcacttaa tatatctttt tgtgttggtt catagctatg taaatctgtc cctgctggat
     2041 agattgatga taccttcacg tgttttccac caatttgctc tgcaaatgat tgcaatggat
     2101 aaactgtcgt tttaattgtt accttgccat ctttatcttt tccatcatca ttcccacatg
     2161 cggctaatga taaagttacg gctggtacaa gaagtaacat acctaatttc tttttcatag
     2221 tcaccctcct aaatagtaat ctttacgttt taaaattata tactcatgct acttaaaata
     2281 caagcactgt ttttataaat aatataaagt cgaatcatac tttaggtatt taccactttt
     2341 cgttttatct aatacatgac tattgtacaa atatgataca gaaaacttta tagtatttac
     2401 gaacgtcata atataagaaa aaaagacaat ctctatcata ttgctaatag aaattgcctt
     2461 ttcatattgt cctaaaataa cttaccctat ttttatagtg tgacttttag atttaataat
     2521 ggtatttaca tgatgctatt tttatatgat tgtcatctac tgtataaacc aatctatgtt
     2581 cgtgattgat acgtctacta taatacccag tcagattcga ttttaacttt tcaggcttac
     2641 ctataccttc caatgcacca tttctgtcaa tacttttaag taactcatta atcttcttca
     2701 ccattttttt atcgttctgc tgaaaatact tataatcttc aaaggcttga ggcgaaaacg
     2761 taatatttaa cctagccatt atacatctat atctttcgtt atagttttcc cacgttctag
     2821 atctgcaatt gattgagcta agtgttcagc attatttggg ttctgttgga ggtaaagtgt
     2881 ttccatcatg gagttataat ctgattctga catgattact acgtttttat catcagtaga
     2941 tgttacagtt accataacac tatcatcatt tacttttgtc ataagtgcct ttaaattctg
     3001 tcgagcgtat gaataatttt taataatcat atttatcgcc tccttttaac acacctcaat
     3061 tgtacaaata tctgtacaat aaaacaagcg acacagaaaa aaactactaa tcaagatttc
     3121 gcacggggaa gaatcaatac ctatcctgta ctgattccct actaaacaat gtttacatga
     3181 taatttccac ctatttagac ttcatacatt aaaaagacaa tctctatcat atcgctaata
     3241 gaaattgcct tttcgtattg tcctaataaa ttggtatcac ttactgatct caacaattaa
     3301 gactatttga ttttatcttt taataacttc tcataacttt cataatcata tggatcttca
     3361 actttctcgc cattaataaa tgcagttggt gtcgttttta tatgattatc tttcgctatt
     3421 tttttatctt tctctgcagc tttccaagac ttgctatctt ttgtcttgta atcttttata
     3481 attttatttt ccgtctcttt atctaaatgc agttgtttaa tatgtttatc taatagttct
     3541 tttgttaacc attctttatt ttcatcttgc tgggcagcaa ataattgctt ttgaaaatct
     3601 aaaaatgatt taggtgcata catcaatact gcatgactcg cacgcgaacc aacaattgag
     3661 tctttaccta agaaagctaa attgacaaat tggtattcca ctttgtgatt atctatataa
     3721 tttttacgca actttggcat gactttttca tctaattctt tacaataagg acatttatag
     3781 tcgccatata cgacaactaa tggtttgccg tttttcgaag atgtcgttgc tgattctttt
     3841 ttaccgcaag ctgttaaaat taacatgctc actataaata atgttagtaa ttttttagtc
     3901 ataataactc ctattcatat ctagttcgtc atattttctt catgataatc cgcatcatgt
     3961 tgttgataat atcgtctcga atcaaaatct tgcttatatg atacacgttt atcactaaaa
     4021 tcatatgcat aataatgcac ttcatcatta ctcaaaggtt tataagctaa aatgataatt
     4081 ttatccttat catagacata aatattagca tctttgcgtt caaaatgatc aacacgatca
     4141 ccgttgcctt ttttggcaag ttgttcttgt ttttgatttt gaattttaac tgcttcatct
     4201 atttctttca catatttatc attaccacat cctgatataa agactagtaa taataatacc
     4261 gtcgccacaa atcgcttcaa tgctcccact cctactatca acttccaact aactttcaat
     4321 ataattaata ctatacaccg tcaaacatta atgcgctaca actactacca atgcatttaa
     4381 tataaatatc caaagaaata acagttatgt atagtcaaag aaaaatatat ttcattttct
     4441 atgtgtattt gtaatcagtt tttgataaaa taaaaaaata gcattattaa taaattcaaa
     4501 aattatagtt caattcagag agggatggga taaaatgaac tttgtaacgt tgacttcgga
     4561 tgagttcaat gcgtttacaa caaagcattt ttcacattac acacaatcag ctattcatta
     4621 caatcataga gttgatttaa aaggcgatgt gcatcttgta ggggttaaag atgacaatgg
     4681 tcaagtgatt gcaggatgct tattgacaga agcacgcaca cttaaatttt tcaaatattt
     4741 ttatacacat cgcgggccag tgatggatta tacaaatcaa tcattagtag catttttctt
     4801 taaagcatta acgtcatatt taaagaaaca caattgttta tatgtccttg tagatccata
     4861 tttaattgaa aatttacgca atgcagacgg tgaaattgtt aaatcttatg ataaccgagc
     4921 atttgttaga acaatggata aattaggtta taaacaccaa ggtttccctg taggttatga
     4981 ttcaatgagc caaatccgtt ggctgtcagt gttagattta aaagataaga ctgaagacca
     5041 acttttaaaa gaaatggatt atcaaacgag acgtaatatt aaaaaaacat atgatattgg
     5101 tgtcaaaact aaaacgttaa cgattgatga aacgcaaact tttttcgact tattccatat
     5161 ggctgaggaa aagcacggtt tcaaattccg tgagttacca tactttgaag aaatgcaaaa
     5221 gttatacgat gaccacgcca tgttaaagtt ggcgtatatt gatttaaacg agtatttaaa
     5281 aacgttacaa ttaaagcaac aacaattaac agctgaactc tcaggtgttg aagaagcatt
     5341 ggaagaaagt cctaattcaa agaaaaataa aacgaaacgc acacagctag aacaacagct
     5401 aaatagcaat aagcgtaaaa tcgacaatac aatagaacaa attgaacaag atggtgcagt
     5461 cttaaactta gcttctgctt tatttatcta caatgagcat gaagtttatt acttatctag
     5521 tggttcaaac cctaaatata atgcttatat gggtgcctac catttgcagt gggaaatgat
     5581 taagtttgct aaagcgcatc atattgatcg ctataatttt tatggtatta ccggtgactt
     5641 ttcagagtca tcagaagact atggcgtcca acaattcaaa aaaggtttta acgcacatgt
     5701 agaagagtat atcggtgact ttattaagcc tatcaaacca ttactatata aggtacaaac
     5761 gtacttaaat cataaacgtc gttaaaatat cgctccaaga ttatgacaac ataacaaaga
     5821 accacgcatc atgtatatgg cttcatcgcc atactaaaac atgatacgtg gttctatttg
     5881 attattcttc atttataaca tttaagaaac gtcttaattc ttctgttttc ggacgattga
     5941 acatctcttc tggagcccct tgttctccga tcatgccttc atgaataaat acaatgttat
     6001 tagatacttc tttagcaaaa cgcatttcat gtgtcacaat gaccattgtc atgccttcat
     6061 tagccaaatc tttaataacc tttaaaacat cattcacaag ttcaggatca agtgcagatg
     6121 ttggttcatc aaacaacatc actttagggt tcattgctag tgctcttgca atagcaacac
     6181 gttgttgttg accacctgat aatgcatgtg gacgttgatc tttgacatgt gttaaaccaa
     6241 ctttctcaag taactcaagt gatttcccac gtgcctcatc ctttttcaac tttttaactg
     6301 tgataagacc ttccattaca ttttctaatg ccgtcttatg cggaaaaagg ttataacttt
     6361 gaaataccat accagactgt ttacgaactt ctatttgtga ttttttatct ttagatgtat
     6421 atgttttgcc gttaacataa actgtacctt cagttggaat ttctaatgca ttaatcatac
     6481 gtaacaatgt tgttttacct gaaccagatc gaccgattaa ggttacaacc tcaccttgtt
     6541 caacagataa atcaatacct ttgatgactt caacatcatt aaatgattta tggatattgt
     6601 tcaattgaat catgagcgat accctctttc aatataagat tcatagatac cttggataat
     6661 tgaaataata aagcatacca cccagtacat tagcgcaact aacacataaa ttgttaaata
     6721 ttcatatgtt gtagacgcaa cttcttgagc ttttctaaac atttcagcca ctaaaataaa
     6781 tcctaataat gatgtatctt tgattaaact taaaaatgta ttacctagtg caggaatcga
     6841 cactcgaatt gcctgcggta aaatgatacg ttgtatcgtt tgaccatacg tcataccgat
     6901 ggaatatgca gcttctgttt gtcctttcgg tatagaaata ataccgccac gaataatttc
     6961 cgaagcatac gcacctacat ttaatgataa accaataata gcagccacta caggtgccaa
     7021 tgtccattgg ttgtcagcgt catttgttaa taatctacct aattctggaa taccataaaa
     7081 tatgataaat agttgtacta tcattggtgt tcctcgaata atagatacat agacacgtga
     7141 aatacttctt aaaattttac tcgttgaaat tcgcattaat gctgtaaata atgcaataat
     7201 caatcctaaa acaaatgtaa ctaatgtaat aggaattgaa tatttgacca aaccttctag
     7261 cataggtccg aaagcttgtt ttgcagcatc tagcgcatgt tgttgttcgc tatttagatt
     7321 tagaaacatc ttgaccaaac catttcttac ctattttagc taattcaccg ttttcctcga
     7381 tttttttcaa gccatcattg aatttttgaa ctgtttcatc atctgctttt ttagaaaatg
     7441 caaatgcaga tctactttgt tcagcattac ctttgattgc tttgatctta gcattaggtt
     7501 tttgtttttt ataatccaag tatgacagac tatcattaaa tgtaccatca acacgcttag
     7561 acaataataa atccattgat tggttaaagc catcaacttt tgtaatatca gcacctttat
     7621 ccttagctaa tttaccataa ttagatgtga atgtttgtgc taacttctta cctttaacat
     7681 catcaaaatc tttaatatct ttttcatttt cacgaataac taaaacagca cttgagaatg
     7741 tgtaaggctt agaaaattta tatttctttt ctctatcagg attaatacct acttggttcg
     7801 cgattacatc aaaacgccct gcgtctaaac ctgcaaacat agaatcccaa gaagtttcat
     7861 taaatttaag ttttaaacct tcttctttag ccactgcttt aataacatca atatcgtaac
     7921 cagttaattt accatcttta tcgtggaaac taaatggtgc ataagtcccc tcagtcccaa
     7981 cattaattgt cttgctatct ttactagctt ccttgtcttt cgacgaattg tttccgcatg
     8041 cagccaatat gaaaacgaaa gctatcatca caaataaaag tcttttcata gaatatatct
     8101 ccttattctt attattctaa tcggaataaa aatattttaa tctgaaatgc cggataagtc
     8161 aatatgttga acttttgcta aaaagcttta tatagctagc aacttatatt tattgacgag
     8221 aatcaacttc ttttttctta ccttgtaaca taaatgaaca aaagaaacta acaactgtaa
     8281 tcaataatgc aaataaaaat ccagcgtgat aaccatgcaa catcgtatca actttaattt
     8341 gattcatcat atcaattttg ttcatgccat tatagtctga catagttgga gaaaagtttt
     8401 tgcttgcatg ggataaaata gtaattaatg cagctgttcc gatagaacca gcaatttgtt
     8461 gtactgtgtt tgtcatagat gaaccatgtg cattcatttc tggctttaat tgattcatcg
     8521 tatgggtcat tactggaatt aatcctaacg cgataccaac cattcgaatc gcataaactg
     8581 ttgccaacat gattgttgat gtttgttcat ccattacaac aaaataagcc gtagtaataa
     8641 caacaatcgc catacctact aaagcaagat ttctaggacc aaacttttca tataaagcac
     8701 ccgaagtcat cgacataaat gccataacaa ttgctcctgg taataaagtt aatccagaat
     8761 ctaaagctga acgatgcaat aaattttgca caaagatagg taaaatcgtt tccgaaccaa
     8821 tcatcgatac cattgtgaca cccataataa tgataccaac tgtaaattgt ttatttttaa
     8881 atacagcaaa atttaataat ggtgttgata gccgatattg acgtaataca aataatgcga
     8941 ctaaaacaac gcctccaatc atagatacta atacgatagg attatcaaaa cctttttctg
     9001 aaattgaact cgttccatac aataacccgc cgaaacctag tacagaaaaa atgacagaaa
     9061 taaaatcaat aggtacttta attgtagttc caacattttt tatcgtttta aatccaatta
     9121 aaaatgtcac agctgcaatt ggagcgacaa catggaaaag cgatctccag ttaaaatatt
     9181 caacaaggta acctgaaaga gtaggtccaa tagctggtgc taaaccaatt accaaaccaa
     9241 atatccccat atatttaccg cgttcatgtg gttggaaaac atccaaaatc gttgtcatca
     9301 tcaatggcat cataatacct gcgcctaacg cctgaataat tctagcaact aataatgttg
     9361 caaagtgcgg actcaagccg ccgagtaatg tacctaagaa aaagatagaa atacccacta
     9421 aaaacacttg tcttgttgta taacgttgta taacaagcgc ggacaaagga ataacaatcc
     9481 cattagtcaa taaaaaagct gttgtcagcc attgaacttg tgtatatgaa atttcaaaat
     9541 ctttcataat acttggtaaa gccgtcacta atagtgtttc atttaataat ccaaaaaatc
     9601 cacccatcag catagtaata atcatcgtta atttttgttt cttcgacatt taaagcttcc
     9661 ccctcaccat ctaaaaaacg acacacattt ttagatatcc ggtaattata cccattttcc
     9721 aaaaaattag cacgacttta ttacaatttc tttaaaacgt aatcattact atttacatat
     9781 gagtagttat cggtcataat atatttacaa ggagggatag agatgaaatt agatttacag
     9841 actgctcgtc gtaatttaaa tagccctaac attaaaacaa gaaagcgtgc tttaaagatt
     9901 attaagcaac ataaaagagc gaaataattg ttttaatttg actatattgt atgtctaact
     9961 atattttcga gtatccctag ctcgttaata taaactctat acttaactat ttccttacat
    10021 tacttcacat atttaagagc tgcatataca ctcactcctt gtgtacatgc agctcttttt
    10081 ttataagtag tatttatcta taacttctaa atcatccgtt aattcataaa caagcggtgc
    10141 acctgtttta atttcataat taatgatatc ttcatctgac acatcttcaa gatatttaat
    10201 caatgcgcga attgaatttc cgtgtgcaga aactaatacc gtttgaccat ctagcaaata
    10261 ttgtgaaata tgatctgtcc aaaatggtat cactcgaact aaagtatctt tcagactttc
    10321 agaataaggc atcatacgtt tatctaaatg attatatcga cgatcagcta agtaagcttc
    10381 acgttgttct tcggtttcag caggtggttt cacatcataa gaacgacgcc aaatatgtac
    10441 ttgttcttct ccaaattctt ttctagcatc atctttattt aagccttgca atccaccata
    10501 gtggcgttca tttaaacgcc agcttttata tacaggaatc cattgttgtt tagattcagt
    10561 taaaatataa tgcgttgtat ctaaagcacg tgttaataac gatgtaaaag ctacatcgat
    10621 ggcaatgtta ttttctctta ctttttcacc tgctctagtc gcttcattaa taccttgttc
    10681 agataaatta acatcttccc atccagtaaa taagttttta gcattccact cgctttgtcc
    10741 atgacgacat aaaattaatt ttggcataac ttataacctc tttcctttaa tctttaggac
    10801 gttatttcag ggatatttta agatgattta atcttagtaa ttttcttttc aatatatttt
    10861 aaaattcaat gattaaatca attatcacta gacattaaat acataaatcc tattccccat
    10921 tttcattttt taattcataa atgaatcaat aaccacctaa taacaaatca tattatacac
    10981 ctttgttcgc tatttttcta aggtttaaaa aatattttta ggtaaaccta aaaatagatg
    11041 taataaaaac gcctcctcag atatttatat atctatgaag acgtttaaat acattataga
    11101 tggtctggtt ctgggtgaac gtatactgag gaaatacctt ttttgtgcaa atgatgttcg
    11161 acattgtcac aaatttgatg cgcttctact aaggaaaggt tagcatctac aacaattgtg
    11221 acatcaataa acacactact tccatggtaa cgccctttaa tacttttaac ttcttgtact
    11281 tcatcaactt ctaaaatatc attgcgatac gcttctaatt cagtttcatt gaaaccatca
    11341 ctcaacataa aaattgcttc tttaaaaata ccaaaaccag tataaacgat tagtaagcct
    11401 agtaatgttg ctaaaataat atcgacaatt gggaaaccga tttgcgtaaa aattaatcct
    11461 atcgctgttc caatgctgac taaactatcc gataaattat ctttggcagc cgaatttaaa
    11521 gaactacttt tcgttctttt cgctagtctt tgattgactg caaatacaat caacattaca
    11581 agaccactga ttaagctgac gataattgtt attgcgttag gtacaacgtc atcttctttg
    11641 aacaaacgag gtgcattttg aataactact tggataccta caaacataat gacaaatgac
    11701 accaataatg aagaaatatt ttcagacttc aaatggccat aaggatgatt tcgatcggca
    11761 ggtttaattg aaattttcaa tccaataata acagctaaag aaacgataat atctgtcata
    11821 ttgtttaatg catcggctct tacagctgca gagttaaaga caaaacccgt gacatactta
    11881 acaatagata agattatata tacaattaaa ctcaaataag caccgcgttg cgccaattta
    11941 agattttcat tatgagacat gcgttgaacc accttgaatt agtatagtaa caatattatg
    12001 aatgattcat tttaatttta caacgttttt aatttttata aatttttata aaattaaact
    12061 aatttattca ttcgcaaccc ctaaaaataa tttttagcct ttctgcgaat tttatgagct
    12121 agaaaggcgc ccaactctcc ctgtttgtta actttcgcct cgaaagtttc tatgttggga
    12181 ccctatgcat tagtagcgaa gctatctaat gtgttccttt attttctgcg aattttatga
    12241 gctagaaagg cttatgcagt tgacgtttta cgtccaactg cgttcctccg tcttcttcaa
    12301 atttatttgt tagaaaggca cccaactctc cctgtttgtt aactttcgcc tcgaaagttt
    12361 ctatgttaga accctatgca tgagttgcga agctatctaa tgtcgtgaac taattatata
    12421 gaagaaaaag tgcatcaatg acaaattaaa tgagatttct actctaccaa actctcttcg
    12481 aaagacaatt ttctcctcta tttattagca aactattgca tttctccata tagtacttcc
    12541 ttacttaaaa tacgctgaat gttctgaatt aaacgctttt ttacatagtt aacactagtt
    12601 aatctattag ttaacattag ttaataatta gttaatttcc atttgtattc tcatgtgata
    12661 aattctaaaa gcatacaata aatttaatat gtaaaaagaa agggaataca catgaaaaat
    12721 aaatatatct cgaagttgct agttggggca gcaacaatta cgttagctac aatgatttca
    12781 aatggggaag caaaagcgag tgaaaacacg caacaaactt caactaagca ccaaacaact
    12841 caaaacaact acgtaacaga tcaacaaaaa gctttttatc aagtattaca tctaaaaggt
    12901 atcacagaag aacaacgtaa ccaatacatc aaaacattac gcgaacaccc agaacgtgca
    12961 caagaagtat tctctgaatc acttaaagac agcaagaacc cagaccgacg tgttgcacaa
    13021 caaaacgctt tttacaatgt tcttaaaaat gataacttaa ctgaacaaga aaaaaataat
    13081 tacattgcac aaattaaaga aaaccctgat agaagccaac aagtttgggt agaatcagta
    13141 caatcttcta aagctaaaga acgtcaaaat attgaaaatg cggataaagc aattaaagat
    13201 ttccaagata acaaagcacc acacgataaa tcagcagcat atgaagctaa ctcaaaatta
    13261 cctaaagatt tacgtgataa aaacaaccgc tttgtagaaa aagtttcaat tgaaaaagca
    13321 atcgttcgtc atgatgagcg tgtgaaatca gcaaatgatg caatctcaaa attaaatgaa
    13381 aaagattcaa ttgaaaacag acgtttagca caacgtgaag ttaacaaagc acctatggat
    13441 gtaaaagagc atttacagaa acaattagac gcattagttg ctcaaaaaga tgctgaaaag
    13501 aaagtggcgc caaaagttga ggctcctcaa attcaatcac cacaaattga aaaacctaaa
    13561 gtagaatcac caaaagttga agtccctcaa attcaatcac caaaagttga ggttcctcaa
    13621 tctaaattat taggttacta ccaatcatta aaagattcat ttaactatgg ttacaagtat
    13681 ttaacagata cttataaaag ctataaagaa aaatatgata cagcaaagta ctactataat
    13741 acgtactata aatacaaagg tgcgattgat caaacagtat taacagtact aggtagtggt
    13801 tctaaatctt acatccaacc attgaaagtt gatgataaaa acggctactt agctaaatca
    13861 tatgcacaag taagaaacta tgtaactgag tcaatcaata ctggtaaagt attatatact
    13921 ttctaccaaa acccaacatt agtaaaaaca gctattaaag ctcaagaaac tgcatcatca
    13981 atcaaaaata cattaagtaa tttattatca ttctggaaat aatcaatcaa aaatatcttc
    14041 tctagtttta catcattttt taaataattt tcgtaacaaa ccgtgattaa aaagaaccgt
    14101 tgattctcaa tcgaatctac ggttcttttt tcattttcca tcaattaaat gcttcttcgc
    14161 tatttgtcag cccacttttt tacctgcaac ttgttaaata atccttacat cgttaacgaa
    14221 tagttcatca tttagttgaa tcagctcaac tttattaact tcatattttc acaaactatt
    14281 gcgcaatcca ttccttttcc actacaagca ccataattaa acaacaattc aataaaataa
    14341 gacttgcaaa gcatagttat gtagctatat aaacgcctgc gaccaataaa tcttttaaac
    14401 ataacataat gcaaaaacat catttaacaa tgctaaaaat gtctcttcaa tacatgttga
    14461 tagtaattaa cttttaacga acagttaatt cgaaaacgct tacaaatgga ttattatata
    14521 tatgaactta aaattaaata gaaagaaagt gatttctatg attaaaaata aaatattaac
    14581 agcaacttta gcagttggtt taatagcccc tttagccaat ccatttatag aaatttctaa
    14641 agcagaaaat aagatagaag atatcggcca aggtgcagaa atcatcaaaa gaacacaaga
    14701 cattactagc aaacgattag ctataactca aaacattcaa tttgattttg taaaagataa
    14761 aaaatataac aaagatgccc tagttgttaa gatgcaaggc ttcattagct ctagaacaac
    14821 atattcagac ttaaaaaaat atccatatat taaaagaatg atatggccat ttcaatataa
    14881 tatcagtttg aaaacgaaag actctaatgt tgatttaatt aattatcttc ctaaaaataa
    14941 aattgattca gcagatgtta gtcagaaatt aggctataat atcggcggaa acttccaatc
    15001 agcgccatca atcggaggca gtggctcatt caactactct aaaacaatta gttataatca
    15061 aaaaaactat gttactgaag tagaaagtca gaactctaaa ggtgttaaat ggggagtgaa
    15121 agcaaattca ttcgttacac cgaatggtca agtatctgca tatgatcaat acttatttgc
    15181 acaagaccca actggtccag cagcacgaga ctatttcgtc ccagataatc aactacctcc
    15241 tttaattcaa agtggcttta atccatcatt tattacaaca ttgtcacacg aaagaggtaa
    15301 aggtgataaa agcgagtttg aaatcactta cggcagaaac atggatgcta catatgctta
    15361 cgtgacaaga catcgtttag ccgttgatag aaaacatgat gcttttaaaa accgaaacgt
    15421 tacagttaaa tatgaagtga actggaaaac acatgaagta aaaattaaaa gcatcacacc
    15481 taagtaaaca gttcaatcat cttaaaaaat cctgggacac ttcatacttg tctcaggatt
    15541 ttttaacaaa ttgaatcagc cttataacat taaattattt tatcgtacat taaatttaat
    15601 aataacaact gatttttata agaataaagt atcgaaccat agtagataca caaataatac
    15661 aaatgaaaca atttaacttg aaagcttaaa taaatattat caagttaata aacaattaat
    15721 ttttagatgg attcatcaaa aatcgtaaaa agcacaattt gtattttaca aacattaatt
    15781 aaaaaagaaa gcaagacatt cgtgcaatcg gttaccttaa attgtttaca actgtcaaca
    15841 ataccaaggt tttattaact atatttctca caaaattagc ttttagcatt ccaaacaaaa
    15901 aaggttaaat cgaacggaat tatggcattt ttaacttaat tgtaaaaaaa gttgataatg
    15961 gtcaattgtt aatgaacagt taattataat aacgcccaaa atatattatt atttaattaa
    16021 gttaaataaa attatagaaa taaagtgaaa cttatgctta aaaataaaat attaactaca
    16081 actttatctg tgagcttact tgcccctctt gccaatccgt tattagaaaa tgctaaagct
    16141 gctaacgata ctgaagacat cggtaaagga agcgatatag aaattatcaa aaggacagaa
    16201 gataaaacaa gtaataaatg gggcgtgact caaaatattc aatttgattt tgtaaaggat
    16261 aaaaaatata acaaagatgc tttgatatta aagatgcaag gattcattag ctctagaaca
    16321 acatattaca actataaaaa aactaatcat gttaaagcta tgcgatggcc attccaatat
    16381 aatattggtt taaaaacaaa tgataaatat gtttctttaa ttaattattt acctaaaaat
    16441 aaaattgaat ctacaaacgt gagtcagaca ttaggataca atatcggtgg taatttccaa
    16501 tcagccccat cactcggtgg taatggatca tttaactatt ctaaatcgat tagctataca
    16561 caacaaaatt atgtaagtga agtagaacaa caaaactcaa aaagtgtttt atggggcgtc
    16621 aaagcgaatt cattcgccac tgaatcaggt caaaaatcag cctttgatag cgatttattt
    16681 gtaggctaca aacctcatag taaagatcct agagattatt tcgttccaga cagtgagtta
    16741 ccacctcttg tacaaagtgg atttaaccct tcatttatcg ccacagtatc tcatgaaaaa
    16801 ggttcaagcg atacaagcga atttgaaatt acttacggaa gaaacatgga tgtcactcat
    16861 gccattaaaa gatcaacgca ttatggcaac agttatttag acggacatag agtccataat
    16921 gcatttgtaa atagaaacta tactgtgaaa tacgaggtca attggaagac tcatgaaatc
    16981 aaggtgaaag gacagaattg atatgaaaat gaataaatta gtcaaatcat ccgttgctac
    17041 atctatggca ttattattac tttctggtac tgctaatgct gaaggtaaaa taacaccagt
    17101 cagcgtaaaa aaagtcgatg acaaagttac tttatacaaa acaacagcca cagcagattc
    17161 tgataaattt aaaatttcac agattttaac atttaatttc atcaaagata aaagttatga
    17221 taaagatact ttagtactta aagctactgg gaatattaac tcaggctttg tgaaacctaa
    17281 tcctaatgac tatgactttt caaaattata ttggggagct aaatacaatg tatctataag
    17341 ctcacaatct aatgattcag taaacgtcgt tgattatgca ccaaaaaatc aaaatgaaga
    17401 gtttcaagtt caaaatactt taggctatac atttggtggt gacattagta tctctaatgg
    17461 tttatctggt ggacttaatg gaaatacagc tttttctgaa acaattaatt ataaacaaga
    17521 aagttacaga acaacattaa gtcgcaacac aaattataaa aatgttggct ggggagttga
    17581 agcacataaa attatgaata atggttgggg accttatgga agagatagct tccacccaac
    17641 atatggtaat gaactcttct tagctggcag acaaagcagt gcatacgctg gccaaaactt
    17701 catagcgcaa caccaaatgc cattattatc tagaagtaac ttcaatccag aatttttaag
    17761 cgtactatca cacagacaag atggcgctaa aaaatctaaa attacagtaa cttatcaacg
    17821 tgaaatggat ttataccaaa ttcgttggaa tggcttctac tgggcaggcg caaattataa
    17881 aaactttaaa actagaacat ttaaatcaac atatgaaatt gattgggaaa atcacaaagt
    17941 gaaattgtta gatacaaaag aaactgaaaa caataaatag ctagtaaaac acggtcgcca
    18001 acagtaattg tgacgaccgt gttttgattt attatcttag taatactgcc attctttttc
    18061 tcaatgtgag atataaagga atagctacaa ttaaagtgaa tattacgcct ggaatcgcgt
    18121 ttaacaacac tacccacaca ggtaaattta aaataataga taatagtagc ctagatacca
    18181 aactgcctaa tacacttgct aaaactaatg atagtacatt tattttcaat aaataaacaa
    18241 ctgcaatagc tataactcta aatataatag agataatcac attaattgga ttaaatacgc
    18301 caaatactag taataatagg ctagataata atccacctaa aaagtacttt ttaattccaa
    18361 agaaagctaa tatcaataat gctgccggtg cagataattg aaaatctaat cctggtataa
    18421 tggacggtat tttcaaaact gccaaaatgg ttaaaatcgc agcaatgaca ctaatttgag
    18481 taatatcttt tgatgtcata ctaaaacccc tataccgttt cataaacaac ttgcttcggt
    18541 gtgctttcta aaaatgatat gtaatgattt aaatcaatac aatcgtccac aaatattatt
    18601 ctgcctccat atctcgtatt aactggttta atatcaaata atcgatggta accaatttta
    18661 gcagcggcaa aataacctgt cgtatacgtt aagtcatcgg acacgcaaag ttctcctttg
    18721 acatacggat gcgcattgat acaactagca attgctaagg catcagtcac tctttcatta
    18781 agatcacctt tattatttat atcttcaaac gaaaaatgtg ttgccctaat ccccctttgt
    18841 ccaaatgaat ctaaacgttt accagatata gcagatagaa taatagctcc tgtataaacc
    18901 gtttcatttt taatatatgt catcccttga tttagcgctt gttcagtgac accacattct
    18961 tgtgttaaat gttggagatt tgctttatca tcctcaataa tttgtaatgc ttttatttgt
    19021 tgaatcggtt ccatgatttt ttgtattttt atattgagaa aatccggttg cccattttca
    19081 tgaaaaaaac ctttattata aaacctttgt accgtctgtt ctattttgtc aaactcacaa
    19141 atcgtttcag caccactaat atgaacatct tgattgctag aacgcatttt tatactatac
    19201 atgacgatca cctcaatctt cttgatgcaa aatttcaaac aacctatcta tatcttgttc
    19261 agtatgaaaa tacgacaatg atattcttaa cattggctta gtcacagttg gataccttaa
    19321 ataacttgta aacacatgat gctttaataa tgtttgatga atgttctcag ccgcttctat
    19381 gtcatcaaac tcaataaact taatcggcga gtttgaacta ttataattaa cattgagtgc
    19441 ttttaacttt tggttaaaat atttactcaa actatttaat ttagtgcgtc tatcatcagc
    19501 atttattaac ttttcaatgt ttctttttat aaaatacaaa ttataaattg gcaaactact
    19561 tgagtagatg agtggtctac cgtgattaat taacatatcc ttcacatcat ttgaacttaa
    19621 aatcacaccc ccgtatgcac cacatgcttt agataaacta gaagtgagta tatctacacc
    19681 ttgataattc gagtaattct ctattccaaa actatgtgaa acatcgagta tcagtgttgc
    19741 gttatattta tgctttaatg agactaattg accaatatcc acaacgtcgc cattcgttga
    19801 aaatacacta tcagatatga ttatttttgg tatattttga ttagggtatt tctctaacct
    19861 tttttctaaa tcagcaatat ctaaatgctt atatatcact ttttctaaac cacttaactt
    19921 aataccgtca ataatactcg catgattttc ttgatctgaa aacacgacac aatttgtatt
    19981 tttgaaaata ttaaataacg ccaaattagc atcataacca ctatttaaga tagtacatgc
    20041 actatatccg agccaacctg ctaacattgt ttcaatttct tcataagctg tcgaacttcc
    20101 actaattaat cttgaacttg ataagtgata actatacttc cgcataaatc tttcgaaatc
    20161 atccttatca aacgctattt gacctaatcc taaataatca ttagatgtat agttcgtaca
    20221 tctcttattt tctacttcaa tatactgtct atctatatac cctaccgatt taagcgaccg
    20281 atataaccct ttctgttgta ataaatcaat ttgctcttga aacttcattc ttgttttcct
    20341 tattttcaca agtgtcataa tcaatttcaa agcctaaatc attaatcata tcgtagtcta
    20401 attggttcgg ttgcccacca gtaattagat aatcaccgac aaatattgaa ttcgccgctt
    20461 ttaatgctaa tggctgtaac gaacgtaagt tgacctctct tcctccagca atacgaattt
    20521 ctttcgtagg attgattaat cggaataatg ctacgattct taaacatttc attggtgtta
    20581 aatcatccat gcttccaaac tttgtgcctt tgattggatg caaaaaatta atcggaatac
    20641 tgtcggcatc catttctttt aaagcaaatg ccatatcaac aatatcttga ttagattctc
    20701 ccataccaca aatcacgcca gaacatggtg atatattatt cgctttcatt agttctatcg
    20761 tatctgttct atctttataa ctatgcgttg tcacgacgtt atcatggtaa ttttcacttg
    20821 tattaatatt gtggttatat ctgtctacac cagctgactt aagtttctta gcttgttcgt
    20881 cattcgttaa tcctaaacat gcacagattt ttagttgcgg gtgttgagat ttaatcgttc
    20941 ttacagtatt actaatatga tcaacttctt tatcgctcgg tcctctacca ctcataacaa
    21001 tacaatatgt tccaatatga ttatcatgtg ccacctttgc tccatcgata atttgttcct
    21061 ctggaattaa agcatatcgc tgtttttgtt taatatctcg tgattgtcca cagtacccac
    21121 aattttcagg acatatacca cttttagcat ttaaaatcat gtttaatttt acttttttac
    21181 caaaataatg ttttcttaaa atgtacgcct catttaataa atctaaggta tcaatattag
    21241 tatcctcata aattttcaat acagtctctt ttgttaattg ttccccttgt aatatgcgtt
    21301 tagccaaatt catattaaca cttcctatct aaaatgttat gtactgcctt tttaaaaata
    21361 cgtaccatcg ttttcacttc tttatttgac atacttaaca ctggcacaaa cgtaatgaca
    21421 ttttctaaat ttctaatcat tagcccgttt tctttacaat tacgtacaat acgttcaact
    21481 ttttcaattt ctaacggcgt tttactatct ttatctgtaa ctaattcaac tccaaacatt
    21541 aagcctcgtc ctcttacatc accaacattt cgatgacgct tcagcgcatg taactgtttc
    21601 tcaagtgttg atgatgtcgt ttcaatatgt gacaataact tacgtttttc ataaagtctt
    21661 atattttcta atgcaaccgt acaaacgatt tgatttccgg tgtatgtatg accatggaaa
    21721 aaggtattca caccatgcga atcacttaaa aatgcattgt atattttttt agatgtcaat
    21781 gtagctgcaa gtggtaagta gccaccagta atcgccttac ctaaacacat aatatccggt
    21841 tgaacatctt catgattgca tgcaaacatc tttccagttc tcccaaaacc aactgctacc
    21901 tcatcacaaa ttaataagac atcgtatttt ttgcacaatt tctcgacttc tttcaaaaag
    21961 cctttaggat gaacaaacaa gcctgtcgca ccttgaatca acggttccaa tataaaccct
    22021 gcgatttcat catttctttc aattagaatt tgctctatat tcgttaaaat agctgtcatc
    22081 atttcatttt cagtatcgta attactgcga tagaaagatg ggctttctac ttgaatattc
    22141 tcaaatatta agtctttaaa tattttatga aaggtcttga taccaccaac acttaccgca
    22201 ccaatcgtat ccccatgata accgtgattt agcgttataa acttgttttt cttggcatat
    22261 ttttctctat caatattttt ccaatactga tatgccatct ttattgcgat ttcaacagac
    22321 gcactgcctg tatcagaata aaatactttt cttagattac ttggcgtgat ttcgattaat
    22381 ttttccgcaa gttctattga cggaatattt gatgatccta gcagcgtaga atgggcaatt
    22441 ttattgagtt gctttttaat taccttattt aagtatttgt tattatgacc atgcacattg
    22501 acccacaacg atgcataacc atctaaatat ttattgccat tcgtatcgta aaggtaacta
    22561 ccctttcctt tttcaatgat gattgcttct tctttgctat atacacccat ttgtgtaaat
    22621 ggatgccaaa catattctga gtctttttgt ttaagttgtt gtgtgtaatt catttttaac
    22681 tcctattaat tgttttaaaa atgcttctga gaaattttca tacgtggcat gttcttcaaa
    22741 tgtatagact gttttatttg ttaattttcc aatcgtcatt tgattgtctt tttcaatata
    22801 gctgtctgta tagcgattca ttattaaaaa attactcgcc gatacattct gattaacata
    22861 atcttggtga acaatggcat cgctaatagc acctaatttt gatggcaaca cactgatgac
    22921 acaatctgca caatcattga ttagatcttt agtcatgtag aaatcatctg taccttcata
    22981 tattggtacg gcaattcccc cagcaccctc aattaagata aaatcaaatt ccttatctaa
    23041 aacttttacc ttatctaata cacgctgctt atttagaaaa atttgatctg tcattttaaa
    23101 tgcaaggtgt ggtgatacag gttgcttaaa agtataaagt gacgttatgt catagcttaa
    23161 atcacattca tttttaaata cttctaaatc tggaaacgtc ccgtcttggc gttcctcagt
    23221 ttgaaatggt ttaaaaatac aaactcgatg accacgtgtt ttcaaagcat ggtataaatg
    23281 ctttgtaaca taggttttgc ctacatcagt attcgtactt gtaataaaaa tcctcataaa
    23341 aataaccacc tcaatccaaa tgtaaacttt tataattaat aagtttacat ttaaggcgca
    23401 tgtttttcaa gaaaacatga atttaaactt ctcagtataa ctataaattt tggcaaaatt
    23461 ttgaccttgc tattgaaatg actaagactt cttaaggaat tcgggcagat ataaacagcg
    23521 attcgataaa atatgtcagt taaaattaat aattgcatta tttcttaata aaaagacatc
    23581 ctagctttac catcttgtga aacaatccat taattgtaat attacgttga gcgccttgtt
    23641 catcaatgct ataattagtt acataataaa ttgaacatct aaatacacca aatcccctca
    23701 ctattgccct agtgagggga tttattttgc tgttgattat ctatcaccct ttttatttcc
    23761 aaattctgat tccatattaa actattattt ataatttcca gcctgctgat ttacttttta
    23821 ttcttataaa gtcttgatac attcctggtt ttcgaattaa ttcatcatga ctaccttttt
    23881 gaattatttc accttcattg agcactataa tctgatctgc atttttaata gtttcaagtt
    23941 tatgtgcaat tgtaattact gttttgcctt tacttaattc attaattgcc gtttgaatca
    24001 gctgttcatt ttcagggtca atacttgcag ttgcttcatc taaaataatt attggtgcat
    24061 cttttaatat cgccctagca atagaaatcc tttgcttttc tccgccagat aaattactac
    24121 ctttttcatt tagcattgtt tgatatccct caggtaatga catgatgaag tcgtggcaac
    24181 atgcttgctt cgctgcacga ataatttctt ctttcgttgc acctggattg ccaaacaata
    24241 tgttattttc aatcgtatca ttaaataaat acaccttttg aaatactgca ctaattttcg
    24301 acattaacgt acttaatgtc atatctttaa tatcaacacc atcgatgcga atatttccat
    24361 catcgatatc ataaaagcgc aagagtaagt gacataatgt agatttccca cttcctgaag
    24421 gtccaattat tgctgttgat gtttgtgtag gtatctcaaa attcacattc tttatcactt
    24481 gtttatcatc atatgaaaag ttcacgtttt gaaaagcgat attttcattt ttaatcgtca
    24541 aattttttcc attctcatcc agttctggag cattttttat cttttcaata tcatctatcg
    24601 tcatatctat catttcaagt acgtgtgcag cactattaat attttcaaca ctatcaaata
    24661 tcacaaatga aaaaattgaa atcataataa gggtaggtaa atcaatacta tgattcatat
    24721 ataataagca tgcaactaat acaatcatta ttgaaacaac ttttaaacta agtaaatgca
    24781 ataaattgaa tgggatatat tgcatttcta tttttgtatt tatacgttta ctttcattga
    24841 ctgcttggtt aaaactttta agactcgtat tttctttcgc gaatgatttt attacttgaa
    24901 taccacgaat aacctccaat accttttcca ctaattggtt ttgtacatta tgatacgctg
    24961 gcgcattttg tcgactcttt ctttctaata attgaatcgc aaaaaatgat agtaatacgc
    25021 caatgcatgc taataatgat acttgccacg aaactacaag tagagacaat atgagtactg
    25081 taattaatat gtatccatta acaacaacgt ccaccatttt catagcaaag ttttctaaaa
    25141 aggttaaatc tgttgttact attgttgtta actcatttga atgatgcgaa ttaaaataac
    25201 ctaaccttac atttttcaat ttatccccta tatctaaacg ttctttcgca ctcatttcat
    25261 aagcgatgct ctcatggctt ttgcttttga aatatgctgt aataaatcgt ccaatcacta
    25321 ataaaaccat gataattaca acattcaata tatctttcat ataaataggt ttatgagata
    25381 gcacattatt aaatattttt gcagctaaaa agataggtaa cgcaataaaa atagcattta
    25441 aaaatgacat gctaaatcct aaaatcattc ttgctttata tggtcttatc cagtttaaaa
    25501 ttttaaatgt aatttgaaac atatttgacc tccttttctc attagtttaa ttatgtccag
    25561 tattgattcc ccaatctttt gtatgcatgt gcgtatccca cattttctta taattaccgt
    25621 ttaattttag caataagtga tgtgaccctt tttctaaaat ttgttgttta cctaaaacaa
    25681 taatttgatc tgcatgttga atcgtagata accgatgtgc aataacaatc aatgttttat
    25741 cctgcgtcaa cacatttaat gcttcttgaa ttttttgttc gttgtctgga tcaacgtaaa
    25801 cagtcgcttc atctaataca atgataggcg catcttttaa tatcattctt gcaatagtga
    25861 ctcgttgttt ttcaccacca gacaatttat ctcccactgt accaacattc gtatcatatc
    25921 catctggcaa cttttcaata aattcatgac attgtgctaa cttggcagct ttttcaactg
    25981 cctcatccgt agcttctgga ttgccaagtt taatattttc tttaaaagta agatttaata
    26041 aaaagttatc ttgtccaaca aaaccaacta aatcgttaag ttgtttcgat tcaatatctt
    26101 ttatatttat accgccaatc gtaatttcac ctgaagtcac atcccagtat cgtgatataa
    26161 gcttggcaat ggttgactta ccgctaccag atgccccgac gatagctgtg aaattatttt
    26221 ctggtactgt aaatgataaa tgcttaaaga ccagatcgtc cttatctttg ttatatgaaa
    26281 atccaacatt attaaaagca atttcataat gttgaggctt cttaaatttt gttgacaata
    26341 ctaactcttc tagacttaat atttgattca cttcagttaa tgcgtattgt atagacttta
    26401 aatgatttac ataattagta aaattcttaa tcggtgctac tacacctaaa gataatacga
    26461 tgcataggaa aaattccgca tagtttaatt ggttgataga tatcaaatac atgccgaccg
    26521 gtaaaatccc taaaaatgtt gaaggtaata cactagctcc taaattcata tacccccatg
    26581 tatttttaaa ccaattcaaa gtgtgaatct tataattatc tactgcatct ttatattttt
    26641 tatatgaact ttgagattga ttaaatgttt taatcacttc aatgccttcg ataaactcta
    26701 caatcgcgct attcatataa ttattcgatt tcatttgttc agcatatgtc tcattaaatc
    26761 cagacataac ttttttaaaa gcgaaaattg aaattggtat cgttactaat aaggcactag
    26821 ccatacgcca atcaatgagc attatgtata aaaagatagc agctgacaaa agtaagtttc
    26881 ctataacttc aggaatcata tgtgctaaag gtaattctat tgtttcaacc ttatcgacaa
    26941 atatattttt taattcacct attttcttag attccactac gcctaaaggg agacgcatta
    27001 atttttgagc taatttttta cgaatttcag ataaaatttc atatgccgta atatgtgata
    27061 gcatcgttga cgctccaaaa caacacactt gtgaaatata agcgattaaa gcaataaaga
    27121 tataaaccat aatcgaatta atcgtatatg tattgttaat catcattaaa ataattttaa
    27181 atactgccca ataaggaact aatccagaaa agacactgat gatagacaac aaaattgata
    27241 acataatttt ccatttacta tcagaagcaa atccgctgag tatctttatc caatttgtct
    27301 gattgttcat cgtatatgta actcctttca attaataatc taaattaagc cgcttatatt
    27361 atttatttca ctgga
//
LOCUS       DAKTGS010000019        26992 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.019, whole genome shotgun sequence.
ACCESSION   DAKTGS010000019 DAKTGS010000000
VERSION     DAKTGS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.019"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            199..798
                     /locus_tag="O0286_000699"
     CDS             199..798
                     /locus_tag="O0286_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0169095.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            919..2994
                     /locus_tag="O0286_000700"
     CDS             919..2994
                     /locus_tag="O0286_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCY0169096.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            3107..3256
                     /gene="rpmG"
                     /locus_tag="O0286_000701"
     CDS             3107..3256
                     /gene="rpmG"
                     /locus_tag="O0286_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0169097.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            3465..4004
                     /locus_tag="O0286_000702"
     CDS             3465..4004
                     /locus_tag="O0286_000702"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCY0169098.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            4016..5479
                     /locus_tag="O0286_000703"
     CDS             4016..5479
                     /locus_tag="O0286_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCY0169099.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            5460..5663
                     /locus_tag="O0286_000704"
     CDS             5460..5663
                     /locus_tag="O0286_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCY0169100.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            5660..6646
                     /locus_tag="O0286_000705"
     CDS             5660..6646
                     /locus_tag="O0286_000705"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCY0169101.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            6646..6975
                     /locus_tag="O0286_000706"
     CDS             6646..6975
                     /locus_tag="O0286_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCY0169102.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            6972..7595
                     /locus_tag="O0286_000707"
     CDS             6972..7595
                     /locus_tag="O0286_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0169103.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            7647..8621
                     /locus_tag="O0286_000708"
     CDS             7647..8621
                     /locus_tag="O0286_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCY0169104.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            8593..9663
                     /locus_tag="O0286_000709"
     CDS             8593..9663
                     /locus_tag="O0286_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCY0169105.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            9677..9988
                     /locus_tag="O0286_000710"
     CDS             9677..9988
                     /locus_tag="O0286_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0169106.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            9966..10412
                     /locus_tag="O0286_000711"
     CDS             9966..10412
                     /locus_tag="O0286_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCY0169107.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            10399..10698
                     /locus_tag="O0286_000712"
     CDS             10399..10698
                     /locus_tag="O0286_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCY0169108.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            10616..11113
                     /locus_tag="O0286_000713"
     CDS             10616..11113
                     /locus_tag="O0286_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0169109.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
                     KTIYV"
     gene            11210..11356
                     /locus_tag="O0286_000714"
     CDS             11210..11356
                     /locus_tag="O0286_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169110.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            11346..11870
                     /locus_tag="O0286_000715"
     CDS             11346..11870
                     /locus_tag="O0286_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCY0169111.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      11857..11954
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12029..13120
                     /gene="gcvT"
                     /locus_tag="O0286_000716"
     CDS             12029..13120
                     /gene="gcvT"
                     /locus_tag="O0286_000716"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCY0169112.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            13140..14486
                     /gene="gcvPA"
                     /locus_tag="O0286_000717"
     CDS             13140..14486
                     /gene="gcvPA"
                     /locus_tag="O0286_000717"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCY0169113.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            14479..15951
                     /gene="gcvPB"
                     /locus_tag="O0286_000718"
     CDS             14479..15951
                     /gene="gcvPB"
                     /locus_tag="O0286_000718"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCY0169114.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(16316..16702)
                     /locus_tag="O0286_000719"
     CDS             complement(16316..16702)
                     /locus_tag="O0286_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0169115.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            16860..17690
                     /locus_tag="O0286_000720"
     CDS             16860..17690
                     /locus_tag="O0286_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0169116.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(17754..17972)
                     /locus_tag="O0286_000721"
     CDS             complement(17754..17972)
                     /locus_tag="O0286_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169117.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(17986..18567)
                     /locus_tag="O0286_000722"
     CDS             complement(17986..18567)
                     /locus_tag="O0286_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169118.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            18672..19733
                     /locus_tag="O0286_000723"
     CDS             18672..19733
                     /locus_tag="O0286_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0169119.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            19759..20316
                     /gene="efp"
                     /locus_tag="O0286_000724"
     CDS             19759..20316
                     /gene="efp"
                     /locus_tag="O0286_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCY0169120.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            20790..21254
                     /gene="accB"
                     /locus_tag="O0286_000725"
     CDS             20790..21254
                     /gene="accB"
                     /locus_tag="O0286_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCY0169121.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            21254..22609
                     /gene="accC"
                     /locus_tag="O0286_000726"
     CDS             21254..22609
                     /gene="accC"
                     /locus_tag="O0286_000726"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0169122.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            22624..22986
                     /locus_tag="O0286_000727"
     CDS             22624..22986
                     /locus_tag="O0286_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0169123.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            23046..23435
                     /gene="nusB"
                     /locus_tag="O0286_000728"
     CDS             23046..23435
                     /gene="nusB"
                     /locus_tag="O0286_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCY0169124.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            23452..24789
                     /locus_tag="O0286_000729"
     CDS             23452..24789
                     /locus_tag="O0286_000729"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCY0169125.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            24782..25012
                     /locus_tag="O0286_000730"
     CDS             24782..25012
                     /locus_tag="O0286_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCY0169126.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            24990..25871
                     /locus_tag="O0286_000731"
     CDS             24990..25871
                     /locus_tag="O0286_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0169127.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            26303..26755
                     /gene="argR"
                     /locus_tag="O0286_000732"
     CDS             26303..26755
                     /gene="argR"
                     /locus_tag="O0286_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCY0169128.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            26771..>26992
                     /locus_tag="O0286_000733"
     CDS             26771..>26992
                     /locus_tag="O0286_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0169129.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESK"
BASE COUNT        10018 a         3575 c         4866 g         8533 t
ORIGIN      
        1 ttcatttcat actccgttcg tatgatttta tggcaaatct gttaactttt tatatacaaa
       61 atgtttaatt atttttgatt tgagttatat taaatgagta gagaaaataa taagtagtag
      121 aatttaagca aattctttgt tgaaccagtc actgcttgtt attatttact tacagacatt
      181 ttaggaggat gattatttat ggcttttgaa ttaccaaaat taccatacgc atttgatgca
      241 ttagaaccac attttgacaa agaaactatg gaaattcacc atgacagaca tcataacacg
      301 tatgttacga aattaaatgc tgcagtagaa ggtacagatt tagaatctaa atctattgaa
      361 gaaattgttg ctaatttaga cagtgtacca gctaacatcc aaactgctgt acgtaataat
      421 ggcggtggac atttaaacca ttcattattc tgggagttac tttcaccaaa ctcagaagaa
      481 aaaggtactg tagtagaaaa aattaaagaa caatggggtt ctttagaaga atttaaaaaa
      541 gaatttgctg acaaagcagc tgcacgcttt ggttcaggtt gggcttggtt agtcgtaaac
      601 aatggccagt tagaaattgt gactacacca aaccaagata atccattaac tgagggtaaa
      661 acacctattt taggtttaga cgtatgggaa cacgcttatt acctaaaata tcaaaacaaa
      721 cgccctgact acattggcgc attttggaat gtagttaact gggaaaaagt tgacgaatta
      781 tataatgcaa caaaataaag tatgattact tgttcggtct cttaaatgag accgttattt
      841 ttttgtcaaa aagatagaaa taatttctaa attcatatat gatttaaagt gaaagacttt
      901 gaatagaggt aggtagtttt gttaaaaaga ctaaaagaaa aatcaaatga tgaaatcgtt
      961 caaaatacaa ttaacaagag aattaacttt atatttggtg tgattatatt tatttttgca
     1021 gtactagtac tacgtttagg ttatttacaa atcgcacaag gctcacatta taaacaaatt
     1081 ataaaaaatg atgaaaacat tacagtgaat gagtctgtgc caagaggtcg tattttagac
     1141 agaaatggga aagttttagt tgataatgct tctaaaatgg ctattacata tactaggggt
     1201 cgaaaaacaa cacaatcgga aatgttggat acggctgaaa agttatcaaa gctaatcaag
     1261 atggatacta agaaaattac agaacgtgat aagaaagatt tctggattca gttgcatcct
     1321 aaaaaagcaa aagcaatgat gacaaaagaa caagctatgt tagcagatgg aagtattaaa
     1381 caagatcaat atgataaaca actgttatcg aaaatcggaa aatcacaatt agatgaattg
     1441 tcttctaaag atttacaagt tttagctatt tttcgagaga tgaatgcagg aacagtttta
     1501 gatccacaaa tgataaaaaa tgaagatgtc agtgaaaaag agtatgcagc agtttctcag
     1561 caactttcca aattaccagg tgttaacacg tctatggatt gggatagaaa atatccatat
     1621 ggcgatactt taagaggtat attcggagat gtatcgacac ctgctgaagg tattccaaaa
     1681 gaattgacag aacattactt atccaaagga tattcacgca atgatcgtgt tggaaaatct
     1741 tacctagaat atcaatatga agatgtattg cgtggtaaga agaaagaaat gaaatacaca
     1801 acggacaaat ctggtaaagt tacatcttca gaagtgttaa atcctggcgc tcgcggtcaa
     1861 gatttgaaat taacgatcga tatagatctt caaaaagaag tagaagcatt attagataaa
     1921 caaattaaga agcttcgcag tcaaggtgcc aaagatatgg ataatgcaat gatggttgta
     1981 caaaatccta aaaatggaga cattcttgcg cttgccggaa agcagattaa taagagtggt
     2041 aaaatgactg attatgacat tggtacgttt acttctcaat ttgcggttgg atcttctgta
     2101 aaaggtggaa cattattagc cggttatcag aataaagcta tcaaagttgg agaaacaatg
     2161 gtcgatgaac cattacattt ccaaggtggt ttgacaaaac gatcatactt caataaaaac
     2221 gggcatgtaa ctattaatga taagcaagct ttgatgcatt catcaaacgt atatatgttt
     2281 aaaacagcat taaaattagc gggagaccct tattattctg gtatggcttt accttcagac
     2341 ataagttcac ctgcccaaaa gctaagaaga ggattaaatc aagtaggctt aggtgtgaaa
     2401 acagggatag atttaccaaa tgaaacaaga ggtcaaatcg aaccattaac aaataatcca
     2461 ggtaattatc tagatttatc aattggtcaa tatgatacct atacaccatt acaattatca
     2521 caatatgttt caactatagc gaatgatggt tatagaatac agccacacat tggattaacg
     2581 attcatgaat caactaataa agatgaggtt ggtccactca agaagaaaat taatggcact
     2641 gtcttgaaca aggttaataa tactgaaaag gaaatcaaac aaattcaaga aggattcaaa
     2701 atggcattta atgataaaga tggtactgga tatgttagtt ttaaagatac agtagtacct
     2761 actgctggta aaacgggtac cgcagaagtg ttccaaaacg gagagccaag agttaactct
     2821 acttatatag gatacgcgcc aattgatgat ccaaaattag cgttttcaat tgtatataca
     2881 aatcagcctg taccaccacc atggttaaca ggtggagact taggtagaga tgtaattaac
     2941 tactacttta agcagttagg taaaaatgat aaaaataaag acaaagacaa ataaaattta
     3001 acctgacgat tgtgtagcgc atggttgtaa aattttaact ttgcaaatat tatagatgtt
     3061 ggtataataa taaagtcgta tttagaaatg ataaggagga ataaccatgc gcgtaaacgt
     3121 aactttagct tgtacggaat gtggtgacag aaactacatt acaactaaga acaaaagaaa
     3181 taatccagaa cgtgttgaaa tgaagaaatt ctgttcacgt gaaaacaaac aaactttaca
     3241 ccgtgaaaca aaataatcat taaaattaaa tttaaattaa caataaaaaa ggtgaaatct
     3301 caaaataaga ggtttcacct ttttaattat gcacacgtat gaaatgataa aagattttca
     3361 attgaaataa taaattttag aattgtcgca taattcatca tgacaacata atgacatgta
     3421 ttgttattaa cgatataata gaattgaata aagtgggtga taatgtgact aaaaatgaga
     3481 ttagaaaata cattttacat aaaatgaaga attttaataa agctgaaaag cgaaaagcag
     3541 acacatggtt aagaaatcaa ttttttgcaa ctgaagaata caaagaagca aacgcaattg
     3601 cgctagttct ttcttttaat catgaagtag atactttttc tattattgaa caagccttaa
     3661 tggatcataa acgtattttt gtaccgaaaa tggattattt aaatcatcaa atgactttta
     3721 aagagatatt taatctcaaa gatattgatg tcgataataa ggggatttac tatccaactt
     3781 caaaaggtga aacaacgaat aacctagatt taattgttgt tcctggtgtt ggatttcaag
     3841 acgatggata tagaattggg tatggtggtg gctattacga caggttttta gctaattatc
     3901 agacaaagac aataagctta ttatacgatt ttcaaataac atcatttgaa ccggaatcat
     3961 tcgatcaacc agtcgataaa ttgattatat atcaagcagc atagtggagg actaaatgaa
     4021 catagacaaa caattttgga aaacaatata ttattggatt aggtacttga attttgatat
     4081 tgtcagtagg gaaaaagatg accaagaaat ttggttagcc cataaaagaa aaaaacaagt
     4141 agtcattttt aaacaacata tcaagtctac ccaagaaata cgttttgaca aagcgaaagt
     4201 gcttgaacac aaagatgaaa tagcaaattt tatttctttc gaaccacaaa gttttgaatt
     4261 ttattatttt acagaatcgg aattttcaga agaacaatta aatgaagttt cgccaattag
     4321 aattaaattc aatgttataa gacacacaaa agatttgata aagcatatgc cgaatatatt
     4381 tttggctaga cttatttcag aagataatga taaaaagaca tatatgttct ataaacgcaa
     4441 agtattaacc gataactttt tagataaata tatgcagaaa ttttcaccgg caacatacac
     4501 aataatattt gtaaatgtct taatatggtt atgtatgatt ttatatttaa ataatttttc
     4561 ggatgtaaaa ttattagatg ttggcgggtt agtgcatttt aatgtcgtac atggtgaatg
     4621 gtatcgaatt gttacatcga tgtttttaca ttttagtttt gaacatatac ttatgaatat
     4681 gctttcatta tttatttttg gtaaaatagt cgaagcaatt attggttcat ggcggatgtt
     4741 aactgtatac tttattgcag ggttgtttgg aaactttgta tcactatcat ttaatacgac
     4801 tacaatttca gttggggcta gtggtgctat atttggtctg attggatcaa tttttgcgat
     4861 gatgtatgtt tcaaaaacat ttaacaaaaa aatgttagga cagttattaa ttgcattagt
     4921 gatattagtt ggtgtttctc tgtttatgtc aaatataaat attgtggcgc atattggagg
     4981 attcattggt ggtttattaa taactttaat tggctattac tataaagtga atcgtaatat
     5041 tttttggatt ttactaattg gtatgcttgt tatatttatt gcacttcaaa ttagaatttt
     5101 tacaattaaa gaagataata tttataataa attgatcaaa gatgatatga ctagtggtaa
     5161 ttatgataat gctcaaaata ttgtgaagca gacaataaat aaaaattatg ccgatgatca
     5221 aacatattat ttaagtggta tgattatggc tactatcaat tctaagtccg aaggtatgac
     5281 agaatgggag cgaggactta gaatgtttcc taaatcaggt ttattaaatt ttgagttagc
     5341 gatagcaaat cgttcattaa atgatgatga aaaagcatta aaatatgtgc gtaaagcatt
     5401 aaatgcagac cctaaaaata cagattatat taacttagaa aaagagttga ctaaatcaaa
     5461 tgagtcgaaa aataaataac ttttatgatg tacaacagtt attgaaaagt tacggatttc
     5521 taatatattt taaaaatcca gaagatatgt acgaaatgat tcaacaggag atttcatcat
     5581 tgtatcaata tgaactgtta tctaaggaag aatatttgaa atgtacgttg ataattaatc
     5641 agagaaggaa tgaacagaaa tgacaaaaat tattttagca gctgatgtag gcgggacgac
     5701 ttgtaaatta ggtattttca cacctgaatt agaacaatta cataaatggt ctattcacac
     5761 tgatacatct gatagtacag gatatacact tttgaaagga atttatgatt cgtttgttga
     5821 aaaagtaaat gaaaataatt ataatttttc aaatgtactt ggcgtaggta ttggtgtacc
     5881 aggtcctgtt gactttgaaa aaggtacagt aaatggagca gtaaacttat attggccaga
     5941 aaaagttaat gtacgtgaga tttttgaaca attcgttgat tgtccagtgt atgtagataa
     6001 tgatgctaac atagctgctt taggggagaa acacaaaggt gctggtgaag gtgccgatga
     6061 tgttgttgcc atcacacttg gtacaggtct aggtggagga attatttcaa atggtgaaat
     6121 cgtacatggt cataatggct ctggcgcaga aataggtcat tttagagcag acttcgatca
     6181 acgatttaaa tgtaattgtg gtcgttctgg atgtattgaa acagttgctt cagcgacagg
     6241 cgttgttaac ttagttaact tctactatcc gaagttgacg tttagatctt ctatattaga
     6301 attgattaaa gaaaataagg ttacagcaaa agctgttttt gatgcggcaa aagctggtga
     6361 ccaattctgt attttcatta ctgaaaaggt tgcaaactat attggatatt tatgtagtat
     6421 tattagtgtt acaagtaatc cgaaatatat cgttctaggt ggaggaatgt ctactgcagg
     6481 acctatttta attgaaaata ttaaaacaga atatcataat ttaacatttg cacctgctca
     6541 atttgaaact gaaattgtac aagcgaaatt aggtaatgat gcaggtatta caggagcagc
     6601 aggattaatc aagacctatg tattagataa agagggggta aaataatggc tattgttgat
     6661 gtggttgtta ttccagttgg aacggaaggt ccgagtgtta gtaaatatat tgcagatatt
     6721 cagaaaaaac ttcaagaata taaagcaatg ggtaaaattg attttcaatt aacaccaatg
     6781 aatactctaa ttgaaggtga attaagcgat gtattagaag ttgtgcaagt gatacatgaa
     6841 ttaccttttg ataaaggttt aagtagagtt tgtacaaata tccgtattga tgaccgacga
     6901 gacaaatcta gaaaaatgaa tgataaacta acatcagtac aaaaacattt agaaaatagt
     6961 ggtgaaaacc tatgaggatt tcaagcttaa ctttaggctt agttgatact aatacgtatt
     7021 tcatcgaaaa tgacaaagct gttattctga ttgacccttc aggtgaaagt gaaaaaatta
     7081 ttaaaaaatt aaaccaaata aataaaccgt taaaagctat tttattaaca catgcacact
     7141 ttgatcatat cggagcagtc gatgatatag ttgatcgatt cgatgtcccg gtttatatgc
     7201 atgaagcaga gtttgatttt ctaaaagatc ccgttaaaaa tggggcagat aaatttaagc
     7261 aatatggatt accaattatt acaagtaagg taactcctga aaagttaaac gaaggtagca
     7321 cagaaataga aggatttaag tttaatgtgt tacacacacc tggacattca ccaggaagtt
     7381 taacatatgt gttcgatgaa ttcgcagttg ttggagatac attatttaat aatggaatcg
     7441 gacgtacaga tttatataaa ggtgattatg aaacgctagt tgattctatt caagataaaa
     7501 tatttgaatt agaaggcgat ttacctttat tccctggaca tggtccatat acgacggttg
     7561 atgatgaaca attaaatcca tttttacacg gttaaattga agtaataaaa acacctccta
     7621 cgtgattata tgtaggaggt gtttttttga agattctatt tcaagaaata attaataaag
     7681 cgatagaaat gaaagcgagt gatgtacatt ttattccagt taaaaatgaa gtaagtatta
     7741 aatttagaat taatgataac ttggagcagt atgaacaaat tgggaatagc atttatcaaa
     7801 agttattagt ttatatgaag tttcaagctg ggcttgatgt ttctacacag caagtcgcac
     7861 agagcggtcg atatagttac cttttcaata aaatatattt tttgagaata tcaactttac
     7921 cattgtcact tggccaagaa agttgtgtta tcagaattgt acctcaattt tttcaaccac
     7981 agaaatcaac ttataaattc aatgatttta aacacctcat gaataagaaa caaggattac
     8041 tattgtttag tggaccaact ggttcaggaa agagtacatt aatgtatcaa atggtctcat
     8101 acgcgaataa agccttgaat ttaaatgtaa tttctataga ggatcctgta gagatgcaaa
     8161 ttcctggtat cgtccaaatt aatgtgaatg ataaagctgg cataaactat gtaaattcgt
     8221 ttaaagctat tttaagatgt gatcctgatg ttattttaat aggtgaaatc agagataaag
     8281 atgttgccaa gtgtgttata caggctagtt taagtggtca ccttgttctg actacattgc
     8341 atgcaactga ttgtaaaggt gctattttaa ggctattaga aatgggcatt tctgtacaag
     8401 aattgataca ggcaactaac ttaattataa accaacgact tgtaactact attaagcaac
     8461 agcgacaatt agtatgtgaa attctatctc agcaacaact ccgatatttc ttttcccata
     8521 atcattcatt accatcatca tttaagaact tagaagataa acttgatgat atgacaaaag
     8581 caggtgtcat ttgtgaaact acaatgcata aatacattta aactacattc taagaagcga
     8641 caattaagta aggcccaaca aatcgactta ctttcaaatt tatgtaattt gttgaaatat
     8701 ggtttcactc tgtatcaaag ttttcaattt ttaaatcttc aaatgacata taaaaataag
     8761 caattaggta ccaccattct aagtgaaatt tcaaatggtg caccatgcaa tcaaatatta
     8821 tcactgatag gttatagcga tactatcgtc atgcaagtat atttggcaga aagatttggc
     8881 aatattatag acgttctaga agaaaccgta aattatatga aagtgaatag aaagtcagaa
     8941 caacgattgt taaagacact gcaatacccc ttaatactag tttctatctt tattgctatg
     9001 attattatat taaacctcac agtaattcca cagtttcaac aattatatac ttctatgaat
     9061 attcaactat cttcttttca aaaaacattg tcttttttca ttaccagctt acctactata
     9121 attgtagtaa tgctcataat agtatctatg ttggctatta ttatgaaatt aatttataac
     9181 aatttaaata tgctcaataa gataaacttt gtgatgaaac taccgctaat atcaggctat
     9241 ttccaattat ttaaaactta ttttgtaact aatgaattag tgttgtttta taaaaatggt
     9301 attacacttc aatcaatagt agacgtttat attaaccata gtagtgatcc atttagacag
     9361 tttctaggta aatacttatt aacttattca gaaatgggat atggtttgcc tcaaatttta
     9421 gaaaaactaa aatgctttaa gcctcaatta attaagtttg tgctacaagg tgaaaagaga
     9481 gggaagctag aagtagaact aaagttatat tcgcaaatat tagtaaaaca aatagaagat
     9541 aaagcgataa aacagactca gtttttacag cctattttat ttttgatttt aggtttattt
     9601 attgtcgcaa tttatttagt aattatgtta ccaatgtttc aaatgatgca aagtataaaa
     9661 taatggaggt taatatatgt ttaaatttct taagaaaact caagcgttta cattgataga
     9721 gatgctatta gtgttattaa tcatcagttt attattaatt ttaatcattc caaatattgc
     9781 taaacaaact gctcacatac aatcaacagg ttgtaatgca caggtaaaaa tggttaatag
     9841 tcaaattgaa gcgtatgcat tgaaacataa tagaaatcca tcgtctattg aagacttaat
     9901 tgcagatggt tttataaaag aagcacaaaa gacatgtaaa tcaggagaga caatcacaat
     9961 tagtaatgga gaagcagttg caaattagaa agcagtcagc atttactatg attgagatgc
    10021 ttgtggtaat gatgttaatc agtatatttc tacttttgac aatgacatct aaaggattaa
    10081 gcaatcttag agtaatagat gatgaggcaa atatcatttc ttttattact gaattgaatt
    10141 atattaagtc gcaagctata gcaaatcaag gatatatcaa tgttagattt tatgaaaaca
    10201 gtgacactat taaagtaata gagaataata atatacgatt tctaaaatta aaagtaggca
    10261 aaataattaa tgttgcaaaa gttgatatta ttgcctttga taaaaaaggg aatatcaata
    10321 aatttggtag cataacaatt tacaataaca attcaattta tagaataata ttccatattg
    10381 aaaaaggaag aattcgttat gaaaagctat aagtgtaaag gttcattctt aatagatagt
    10441 atggctggat ttttgctaat tggattgatt acattactat tgataccaat gatgaatcaa
    10501 atgcaagcga gtataaacca taaactacaa acaattgatg cttctaaagt aattttgacg
    10561 actgtatcta aaattaataa agaagaactt aagaaggggg taactatagg gaagtatgat
    10621 attaagcaaa gtgaccaaca aatttgtgct atttcaaaaa ataccacttc ttatcaaaag
    10681 acatgtatac agtattaatg tcaaagcttt ttcgctcatt gaaatgttag tagcgatgat
    10741 ggttataagt ataactttac taattgttcc agacttaatt agacttagta aaacttttct
    10801 aattgaaagt agggatttaa caactgtaga tttcgaattt ttctcaagag atattctaga
    10861 tgattttaaa ggagtagata gaaacgatat tgaaattagg caacaccgta tcattttaca
    10921 taaaggtgaa gaaatgatcg aatacaaatt aattaataat aaaattatta aagttgtaaa
    10981 tgacagagga aatataacaa tgattaataa tgttacagca tttactgcaa atatctacta
    11041 taaatccatt attaaaataa cgataacagt taaagtcggt acaaatgtgc agactaaaac
    11101 tatttatgta taacttacta gtatttaaat gagtactatt aaaaaggatg tgaaataatt
    11161 tattaatttt gatttatata aagaaaaagg atatgttacg ccgttcttat tgatgctaac
    11221 agcaatttat ttttccattt tcacttttta tatcagtcaa tatagtttaa aattaaagac
    11281 attatataat attgaaactg actataacta taagattgtt ttgttattga aagaagggaa
    11341 aacgcatgaa tcatgataaa tcaccaataa ttttaattgg tttcatgggt accggtaaat
    11401 ctacgattgg taaatacgtt gcagatgagc aaaatttatc atttattgat atagattcat
    11461 atatcgaaga gaagtataag ttaacaatac cagaaatatt ttgtaaacat ggtgaacaat
    11521 atttcaggaa tttagagttc acatgtttgc aagaatgtat taacactgca gatataattg
    11581 ctactggtgg tggtattatt gagagtgaag aggcatttaa ttttttgaaa aatcaaaaaa
    11641 acattatttg gttagattgt aatattgata ttatatatag tcgaatcaat gatgacccac
    11701 atcgacctaa tgcaaataat aagacaatca agcagttaaa tgacttgtat tgctcgcgga
    11761 atttaagata taatgaaatc gcattcaaga aatttgatag tcatttgcta tcaatttcag
    11821 aaatatatta tgaattgcta aatttaataa aagcgagtga tcagtattag agagaataga
    11881 gcgttaagac tctatcgccg aaggtgcaag taatttatta cgaaactctc aggcaaaagg
    11941 ataatactgt aacgcgttcc tgaattggtg atttataaac agggtagcga ttgctatcct
    12001 gtttttataa ttttaagggg gtatttcaat gtcaagtgat ttaaaacaaa cacctttata
    12061 tcaaaattat gttgatagag gtgcaaaaat tgtggaattc ggaggatggg cgatgcctgt
    12121 tcaattttca agtattaaag aggagcataa tgctgttcga tacgaaattg gcctgtttga
    12181 tgttagtcat atgggtgaaa ttgaagtaac aggtaaagat gctagtcagt ttgtgcaata
    12241 tttattatca aatgatactg ataatttaac tacttcaaaa gcattatata ctgctttatg
    12301 taatgaagaa ggcggtatta ttgatgattt agtaatatat aaattagctg acgacaatta
    12361 tttattagtt gttaatgctg ctaatactga aaaagatttt aattggattt taaaacacaa
    12421 agagaaattt gatgttgaag tacaaaatgt atcaaaccaa tatggtcaat tagcaataca
    12481 aggaccaaaa gctagagatt taattaatca attagttgat gaagatgtaa ctgaaatgaa
    12541 aatgtttgaa tttaaacagg gtgtcaaatt atttggagca aacgtcattt tatctcagtc
    12601 aggttacaca ggtgaagatg ggtttgaaat ttattgtaat atcgatgata ctgaaaaaat
    12661 ttgggatggt ttattagagt ataatgttat gccatgtggg ttaggcgctc gtgatacatt
    12721 aagattagag gctggattgc cattacatgg acaagattta actgaatcaa ttacaccata
    12781 tgaaggtggt atcgcttttg caagtaaacc attaattgat gctgatttta ttggtaaatc
    12841 tgtattaaaa gatcaaaaag aaaatggtgc accaagaaga acagtgggat tagaattact
    12901 tgaaaaagga attgcaagaa ctggttatga agttatggat ttagatggaa atattattgg
    12961 agaagtaact tcaggaacac agtctccatc atcaggaaaa tcaattgcac ttgcaatgat
    13021 aaaaagagat gagtttgaaa tgggtagaga gttgcttgtt caagttcgta agcgtcaatt
    13081 aaaagcgaaa attgttaaga aaaatcaaat tgataaataa ttaaaaaggg gtgtgcattg
    13141 tgagtcatcg ttatatacct ttaactgaaa aagacaagca agaaatgtta caaacaattg
    13201 gtgcaaaatc tataggagaa ttattcggtg atgtaccaag tgacatttta ttaaatagag
    13261 atttaaatat tgctgaaggc gaagcggaaa caacgttact tagaagatta aatcgcattg
    13321 caagcaagaa tatcactaaa gaaacgcata catcattttt gggtgctggt gtttatgatc
    13381 attacgcgcc atcagttgtg gatgctatga tttcaagatc agaattttac acagcatata
    13441 cgccatatca accggaaatt tctcaaggtg aattacaagc catttttgag ttccaaactt
    13501 taatttgtga attaacagat atggatgtag caaattcttc tatgtatgat gggatgacta
    13561 gctttgctga agcatgtatc ttagcattta gtcaaacaaa gaaaaataaa attgtagtct
    13621 caaaaggttt acattatcaa gcattacaag tacttcatac ttatgctaag acgcgtaagg
    13681 aattcgaagt agtggaaatc gatttagatg gcactgttac agatttgaaa aaattagaag
    13741 cagcagtgga tgatgaaaca gcagctgttg cagtacaata tccaaacttt tacggttcaa
    13801 ttgaagatct tgaaaagatt catagcttta ttgaagataa aaaagcatta tttatcgtat
    13861 atgcaaatcc attagcattg ggattactta cacctccagg ttcatttggt gcagatattg
    13921 ttgtaggtga tactcagcca ttcggaattc ctgctcaatt cggtgggcca cattgtggat
    13981 attttgcaac gacgaaaaag ttaatgcgta aagtgccagg tagattagtc ggtcaaacac
    14041 aagatgatga aggtaatcgt ggctttgtat taacattaca agcacgtgaa caacatattc
    14101 gacgcgataa agcgacatct aatatttgtt ctaaccaagc attaaatgct ttagcgtcat
    14161 caattgcaat gtcagcactt ggtaaacaag gtatctatga tattgcagtt caaaatatag
    14221 aacatgctaa ctatgctaaa caacaattta ttaaaaaagg ttttgaagta ttagatggta
    14281 catctttcaa tgagtttgta gtcaaatttg acaaaccaat tcaacaagtt aacgaggaat
    14341 tagtgaagta caatattatc ggtggttttg atttaggtgt tgtttcagac gactttaaaa
    14401 atcacatgtt aatagctgta actgaattaa gaacaaaaga tgaaatcgat acatttgtag
    14461 aaaaggctgg tgagttaaat gactagtaaa tcaagtccat taatttttga aagatctcgt
    14521 gaaggcagat atgcatattc attaccaaaa agtgatatta aaacaaattc tgttgagtca
    14581 ttgttagatg ataaatttat tcgtaaaaac aaagcggagt ttcctgaagt tgctgaacta
    14641 gacttagtac gtcattatac tgaactttca aataaaaatt tcggtgtaga caacggattt
    14701 tatccattgg gttcatgtac gatgaagtat aaccctaaaa ttaatgaaaa ggttgctaga
    14761 ataccaggat ttagtgaatc acatccatta caagatgaag atcaagttca aggttcatta
    14821 gaaattattt atagtttgca agaagaatta aaagaaatta ctggtatgga tgaggtgaca
    14881 ttacaaccag ctgctggcgc acatggtgaa tggactgcat tgatgatatt taaagcttac
    14941 catgagaata atggtgaagg tcatcgtgat gaagtcattg tgccagattc tgcgcatggt
    15001 acgaatccag cctcagcttc atttgcagga tttaaatcag ttactgtaaa atcaaacgaa
    15061 cgtggcgaag ttgatattga tgacttgaaa cgtgttgtaa atgaaaatac agcagctatt
    15121 atgttaacta atccaaacac tttaggtatt ttcgaaaaaa atattatgga aatccgtgaa
    15181 atcgtccata atgctggtgg tctattatat tatgatggtg cgaatttaaa cgctattatg
    15241 gacaaagttc gcccaggaga tatgggattt gatgctgttc atttaaactt gcataaaaca
    15301 tttactggtc cacatggtgg tggcggtcct ggttcaggtc cagtcggtgt agtaaaagaa
    15361 ctagcaagtt acttaccaaa gccaatggtt attaaagatg gcgacaaatt taaatatgat
    15421 aatgacatta aaaattctat cggacgtgta aaaccatttt atggtaactt tggtatttac
    15481 ttaagagctt atacgtatat tcgaactatg ggagcaactg gacttaaaga ggtttctgaa
    15541 gcagcggttc ttaatgcgaa ttatattaaa gcacgtttat ctaaacactt tgaaatacct
    15601 tataaacaat attgtaaaca cgagtttgtg ttaagtggtg tgcgtcaaaa agaatttggt
    15661 gtacgtactt tagacatggc taagcgatta ttagatttcg gtgtacatcc accaacaata
    15721 tacttcccat taaatgttga agaaggtatg atgattgaac cgactgagac agagtctaaa
    15781 gaaacacttg attattttat cgatacatta attagtattg ctgaagaagc taaaaatgat
    15841 cctgataaag tgctagaagc accacataca actgtgattg atcgattaga cgaagctaca
    15901 gctgctcgta aaccaatatt aaagtttgaa aatcttaaac aggaaaaata aagtattaaa
    15961 cacatattcc gagaattatt attctaactt tgtatgaaga tttaaggata atggtttcaa
    16021 aatcaattga aaaagacaat ttctatttaa acaagaaaac taaaccgaag taataactct
    16081 tagggtttgg tattattctt tcatagaaat tgtcttttca ttttttagat tgcggtaatt
    16141 gaatcgtatt gaaaatgagc tgaactttct ttattatgct gaaactaagt ttaatgatca
    16201 gtgaagctag agaaatatag taacagactt tgttaaaaaa ttaaaatctg agtattgtta
    16261 ttgatgatca attaacactc agattttatt aaatcgtata ttaaatttac aaaaactatt
    16321 ttttagactt tatttttcca gtccattttt tatagccgcc ttttaacata tagatatctg
    16381 tatatccatt ctttttcaaa atacgagcgg ctctatagct agcaatccca ttggcatcac
    16441 ataagtatac cggttgatct tttcttaatc cttggaatcg ttgcctgaac attgtcatag
    16501 gaatattgcg agacccatta atgtgaccgt agtcatagtc aactttctct ctaacatcga
    16561 tgacttgagc ttttctaatc ccattatgga attcattttg atctaattct ttaacagctc
    16621 gcttgttaag aatttgttga acaatcatat aagcaataat tgctataact aaaattattg
    16681 cgatgtacaa actagcactc atcttgcatc ctccctaatt aaacgataat ataattataa
    16741 gactgttagg tcaaattatc aaaatatttc actaaagaat tgcaattatt attcatttag
    16801 ttaaattgat taattagcga atcaaatatt atacattttg ctgataggag ttcttgaata
    16861 tgactgaaac ttggaatttt attaatacag gaagtaaaga cccatattat aatatggcaa
    16921 tggatgaagc gttattaaat tttgtctcta gaggtgaaat tgatccagtt atacgttttt
    16981 acacatggaa tcctgcaaca ttatcaatag ggtatttcca aaggctacaa aaagaaattg
    17041 atattgataa ggttaaagag aaaggttttg gtctcgtaag gcgtcaaact ggtggccgtg
    17101 gggtgctaca tgataaagag ttaacttaca gtgtgattgt gcctgaatcg catccgaata
    17161 tgccttctac agttacagaa gcgtacagag tgatttcaca aggattatta gaaggattta
    17221 aaaacttagg atttgatact tattttgcgg tacctaaaac gcctgaagag cgtcaaaaat
    17281 taaaacaacc tcgaagttca gtgtgttttg atgcaccaag ttggtatgag ctagttgttg
    17341 aaggccgtaa gattgctggg agtgctcaaa cgagacaaaa gggcgtaatc ctccaacacg
    17401 gttcgatttt acaagatatt gatattgatg aactgtttga tatgtttatt tataaaaatg
    17461 aaagattaaa attaaaaatg aaagaggcat tcgttgaaaa agcagttgcc atcaatgata
    17521 tttcagatga acacattact attagtcaaa tggaagaagc tttcgagaaa ggttttaaga
    17581 aaggtttaaa tatagaattt aagccacttg aattaacaga agctcaattg gcagaagtgg
    17641 aagaattaac tgaaaaatat cgttcagatg aatggatgtt tagaaagtag tttttagaat
    17701 aaaaaatgga agcacgtaga ctattttacc tacaagtgct tccgtttttt tatctatttt
    17761 cttctttgat ttcttttata cttacgcatt gctttgcgat atttgcgttg gtcttcagtt
    17821 aagatgaaga aataataaat cagataaata ataccagcta ttacagcaaa gctgattaac
    17881 attctgacga ttgaaaaaat aaaggcatct agattcatta ctaatccaat cgcagcaata
    17941 ataagtacaa gataaaatat tatattacgc attaaaagac tcctatcatg attttgcatt
    18001 taagtttaat tttgacaacg cttgttgtcc ttttgtaagt tgttttttat ctttattttg
    18061 atagccattt ttaatatcat caaatgcatg attcaaatca gagtttaatt tatcgatatt
    18121 tttagacgtt tttttatcat catcactaag tgataaacca tcgatagcat ctttgtatga
    18181 agtgtgagca ctttgaatat cattagaaat ttgatttaat ttcgattgaa cattcttatc
    18241 gcctttgttt ttacttacat ctttctcaat atcgttatag gatttcaaag ctttagcaac
    18301 tttgtcgtag tatgttgatg taacattcat caatttaatt ttcgtattat tagatgcagt
    18361 ttttttactg ttttctttat ccttttcaag ccctttaatt ttagatttag aatcactaat
    18421 ttgttgatta agttcttgaa tatctagttt gagttgatga ttatcttctc ttaaatctgt
    18481 tgttttttct tctaatggtg ctaaattttg tgatccacat ccagctaaca ataatgttgc
    18541 gccaacaatt gaaaccaatt ttttcattac tatctcccta aaatcaatat ctattatttt
    18601 ttatccttat tatgatatta ttttaatgta taaagttgaa atgcacaaac gtttcactag
    18661 gaggtaataa gatgagcaga ataacacaag tgcatcgtat tttagaacaa aaacatttag
    18721 atgcgattat cattctttcg gattacaata gaagatattt atcaggtttt actggtacca
    18781 gtggagcact tatcatatct aaagataaac aatatttgat aacagacttt agatatattg
    18841 atcaagcaac taagcaggcg ccaaattatg aaattattaa tcgtaaatct actattattg
    18901 gtgagattaa agaattgcta caccaagaaa attttgaaaa tgtaggtttt gaggggcatc
    18961 atgtaagtta tgatacatac cttgaattaa ataaaagccg tatatcatta ataagcattt
    19021 ctaatactgt agataaaatt agagacgtca aagatgctga cgaaattgct ttaattcaaa
    19081 aagcagctaa tattgttgat gaaacatatg aatatatttt aactgttgta aaagcaggca
    19141 tgactgaaaa agaattaaag gcaatattag aaagcaaaat gctagaatta ggagcagatg
    19201 gaccatcatt cgatacgatt gtagcatctg gtcatagagg tgcattacca catggtgttg
    19261 caagtgataa aattattgaa aaaggcgaca tgattacatt agattttggc gcgtattata
    19321 acggctattg ttcagatatt actagaacat ttgctattgg agaaccagat cctaaactga
    19381 aagaaatata tcaaatagta cttgaatctc aaatgaaagc aattaatgag attagacctg
    19441 gcatgactgg tgcagaagct gatgccattt caagaaacta tttagagtca aaagggtatg
    19501 gaaaagaatt tggacattca ctaggacatg gtattggttt agaaatccat gaagggccaa
    19561 tgctggctcg tacgatacaa gataaacttc aagttaacaa ctgtgttaca gtagaacctg
    19621 gtgtttatat agaaggtttg ggcggtataa gaatagaaga tgatatttta attacagaaa
    19681 atggttgtca agtctttact aaatgcacaa aagaccttat agttttaaca taagcgtgta
    19741 aaatgaggag gaaactgaat gatttcggtt aatgatttta aaacaggttt aacaatttct
    19801 gttgataacg ctatttggaa agttatagac ttccaacatg taaagcctgg taaaggttca
    19861 gcattcgttc gttcaaaatt acgtaattta agaactggtg caattcaaga gaaaacgttt
    19921 agagctggtg aaaaagttga accagcaatg attgaaaatc gtcgcatgca atatttatat
    19981 gctgacggag ataatcatgt atttatggat aatgaaagct ttgaacaaac agaactttca
    20041 agtgattact taaaagaaga attgaattac ttaaaagaag gtatggaagt acaaattcaa
    20101 acatacgaag gtgaaactat cggtgttgaa ttacctaaaa ctgttgaatt aacagtaact
    20161 gaaacagaac ctggtattaa aggtgatact gcaactggtg ccactaaatc ggcaactgtt
    20221 gaaactggtt atacattaaa tgtaccttta tttgtaaacg aaggtgacgt tttaattatc
    20281 aacactggtg atggaagcta catttcaaga ggataatctc taatttgtta acaaatagct
    20341 tgtattcact atactgattt aacgtaagat attctaaata agtctcataa agctattgcc
    20401 taaaatgatt ataggttata tgctgatatg aggcttttta tttttaaaat aatttttaaa
    20461 atataaaacg aatcgtctaa ttggtagtga aaaaacatag acatgagtag aggtatatat
    20521 ctacatatca tattgttgag gataaaacat aatagctaat aaaaataaca ttgatatgtt
    20581 taactgaatg cataaaattt ttaatcaaga gttaagatga gtcaatgtta agtaataaat
    20641 acacaagtgt ttttaattta aaatgtggtt ttattataac tttgggatag acttataatg
    20701 tcgccagcaa tatgaacatg cttgaattga agagttgtct caagtaaaat agacgggtag
    20761 atgaaaacaa actgaaggag tcagtaataa tgaactttaa agaaatcaaa gaattaattg
    20821 aaattctgga taaatcaact ttaacggaaa tcaatattga agatactaaa ggcaaagtga
    20881 cgcttaagaa agaaaaagaa actgagatta tcacgccaca aatctcacaa atgccagttg
    20941 aagctgcggc aatgcctatg cctcaagcac aatcaactga tagcaataaa actgaagctc
    21001 caaagccaac ttcagataat cacaaaacaa ttaatgcacc tatggtaggt acattttaca
    21061 aatcgccatc tccagacgaa gaagcatatg tgcaagttgg ggacactgtt tcaaatgaaa
    21121 caacagtgtg tattttagag gcaatgaaac tatttaatga aattcaagca gaaatttcag
    21181 gtgaaattgt tgaaatctta gtagaagacg gacaaatggt agagtatggc caaccgttat
    21241 ttaaggtgaa ataatgaaaa aggttttaat tgcaaaccgc ggtgaaatcg cagttaggat
    21301 tattcgcgct tgtcgtgatt taggcatcca aactgttgca atctattctg aaggggataa
    21361 agatgcgcta catactcaaa ttgctgatga agcatattgc gtaggtccca ctttgtctaa
    21421 agattcatat ttaaatattc cgaacatctt atctattgca acttctacag gttgtgatgg
    21481 cgttcatccg ggttatggct ttttagctga aaatgctgat tttgcagaat tatgcgaagc
    21541 atgccaattg aagttcattg gaccaagtta tcaatctatc caaaaaatgg gtatcaaaga
    21601 tgttgctaag gcagaaatga tcaaagccaa tgttccagtt gttcctggta gtgacggttt
    21661 aatgaaagac gtctcagaag ctaagaaaat cgccaaaaaa attggctatc cggtcatcat
    21721 taaagctact gctggcggtg gcggaaaagg tatccgtgtt gctcgtgatg aaaaagaact
    21781 tgaaactggc ttccgaatga cagaacaaga agctcaaact gcatttggta atggtggact
    21841 ttatatggag aaattcatcg aaaacttccg ccatattgaa atccaaattg ttggggacag
    21901 ctatggtaat gtaattcatt taggagaacg tgattgtaca attcaaagac gtatgcagaa
    21961 attagtggaa gaagcacctt ccccaatttt agatgatgaa acacgtcgtg aaatgggaaa
    22021 tgccgcagtt cgtgcagcga aagctgtaaa ttatgaaaat gcgggaacaa ttgagtttat
    22081 atatgattta aatgataata aattttattt tatggaaatg aatacacgta ttcaagtaga
    22141 acatcctgta actgaaatgg taacaggaat tgatttagtt aaattacaat tacaagttgc
    22201 tatgggtgac gtgttaccgt ataaacaaga agatattaaa ttaacaggac acgcaattga
    22261 atttagaatt aatgctgaaa atccttacaa gaactttatg ccatcaccag gtaaaattga
    22321 gcaatatctt gcaccaggtg gatatggtgt tcgaatagag tcagcatgtt atactaatta
    22381 tacgataccg ccatattatg attcgatggt agcgaaatta atcatacatg aaccgacacg
    22441 agatgaagcg attatggctg gcattcgtgc actaagtgaa tttgtggttc ttggtattga
    22501 tacaactatt ccattccata ttaaattatt gaataacgat atatttagaa gcggtaaatt
    22561 taatacaaac tttttagagc aaaatagcat tatgaatgat gaaggttaat aggaggtcaa
    22621 tccatggtca aagtaactga ttattcaaat tcaaaattag gtaaagtaga aatagcgcca
    22681 gaagtgctat ctgttattgc aagtatagct acttcggaag tcgaaggcat cactggccat
    22741 tttgctgaat taaaagaaac aaatttagaa aaagttagtc gtaaaaattt aagccgtgat
    22801 ttaaaaatcg agagtaaaga agatggcata tatatagatg tatattgtgc attaaaacat
    22861 ggtgttaata tttcaaaaac tgcaaacaaa attcaaacgt caatttttaa ttcaatttct
    22921 aatatgacag cgatagaacc taagcaaatt aatattcaca ttacacaaat cgttattgaa
    22981 aagtaatgtc atacctaatt cagtaattaa ataaagaaaa atacaaacgt ttgaaggagt
    23041 taaaaatgag tcgtaaagaa tcccgagtgc aagcttttca aactttattt caattagaaa
    23101 tgaaggacag tgatttaacg ataaatgaag cgataagctt tattaaagac gataatccag
    23161 atttagactt cgaatttatt cattggctag tttctggcgt taaaggtcac gaacctgtat
    23221 tagacgagac aattagtcct tatttaaaag attggactat tgcacgttta ttaaaaacgg
    23281 atcgtattat tttaagaatg gcaacatatg aaatattaca cagtgataca cctgctaaag
    23341 tcgtaatgaa tgaagcagtt gaattaacaa aacaattcag tgatgatgat cattataaat
    23401 ttataaatgg tgtattgagt aatataaaaa aataaaattg agtgatgtta tatgtcagat
    23461 tatttaagtg tttcagcttt aacgaaatat attaaatata aatttgatca agatccacat
    23521 ctccaatctg tattgattaa aggtgagctt tcaaatttca aaaagcattc aagtggtcat
    23581 ttatacttta atgtgaaaga taaagaaagc gttataagtg ccatgatgtt caaaggtagt
    23641 gcttctaaat taaacttcga acctaaagaa ggagatgaag tcttattaga agcacgtgtt
    23701 tctgtttttg aacgtcgtgg aaactatcaa atttatgtaa ataaaatgca attagatggt
    23761 atagggaact tatatcaaaa attagaagca ttaaagaaaa aattaactga agaaggttgt
    23821 tttgataaag cgaataagaa atcaatacct aagtttccta aaaaaattgc agttttaaca
    23881 gcgagtacag gtgccgcaat tcgagatatc cattcaacga taaatagtcg ttttccatta
    23941 gctgaacaaa tacaaattag tactttggtt caaggtgaaa aagcaaaaga cgacattatt
    24001 gaaaaaattg aatatgcaga tagtttaggt gtagatacca ttattgtagg tcgaggcggt
    24061 ggatccatag aagatttatg gaatttcaac gaagaagctg tcgtccgtgc aatatataat
    24121 tgtaagacac cgattatatc agcagttggt catgaaacag actttacatt aagtgatttt
    24181 gctgcagaca tcagagctgc gactccaact caagctgctg ttattgcaac accagaccaa
    24241 tatgaattgc tgcaacaaat acagcaatat cagtttacat tgactcgttt catcaagaaa
    24301 catttagaac aacaacgtaa acatgttgaa catttgtcat cgtattacaa gtttaaacaa
    24361 ccaactttat tgtatgatca acagatacaa cgtcgagatg atttagaaaa gagactgaaa
    24421 caacaaattc aggcaacttt tgaacaacag agacatcgat taatgctgtt gcaacaacgt
    24481 tataatttaa aagcattact aagtagtgtt aatcaagaac aacaaaataa tcttcaattg
    24541 acaaaccaac ttgttaaatt attgaattct aagatattaa gttataaaaa tgatttgaaa
    24601 aataaagtag aaaacttaaa caatttaagt ccaactaata caatgttgcg tggatatgca
    24661 attgttaata aaaaagacga agtcattacg agtactaaag atttaacaga aaatgatcaa
    24721 ttgacattaa caatgaaaga tggcttagta gatgcaaaag ttacgaaagt aaggtgtaat
    24781 aatgactaaa gaaacgcaaa gttttgaaga aatgatgcaa gaattagagc aaattgttca
    24841 aaaattagat aatgaaacag tatctttaga ggaatcatta gatttatatc aacgtggtat
    24901 gaaactatca gcagcttgtg acacaacttt aaaaaatgcc gaaaaaaagg tgaatgactt
    24961 aataaaagaa gaagctgagg atgtaaaaaa tgacgaatct accgatgaat aaattaatag
    25021 atgaagtcaa taatgaatta tcggttgcga taaataaatc agtaatggat actcagctag
    25081 aagaaagtat gttgtattca ttaaatgctg gaggtaaacg catccgacca gttctgttat
    25141 tactcacttt agattcacta aataccgagt atgagttagg tatgaagagc gcaattgcac
    25201 tagaaatgat tcatacatat tcacttattc atgatgacct accagcgatg gataatgatg
    25261 attatcgacg aggaaaatta acaaatcata aagtatatgg tgagtggact gcgatattag
    25321 caggtgatgc tttattaact aaagcatttg aacttatttc aagtgatgat agattaactg
    25381 atgaagtaaa aataaaagtt ctacaacggc tgtcaatagc aagtggtcat gttggaatgg
    25441 tcggcggtca aatgttagat atgcaaagcg aaggccaacc aattgatctt gaaactttgg
    25501 aaatgataca caaaacaaaa acaggagcat tattaacttt tgcggttatg agtgcagcag
    25561 atatcgctaa tgtcgatgat acaactaaag aacatttaga aagttatagt tatcatttag
    25621 gtatgatgtt ccagattaaa gatgatttat tagactgcta tggtgatgaa gcaaagttag
    25681 gtaaaaaagt gggcagcgat cttgaaaata ataaaagtac gtacgtgagt ttattaggga
    25741 aagatggcgc agaagataaa ttgacttatc atagagacgc agcagtggat gaactaacgc
    25801 aaattgatga acaattcaat acaaaacact tattagaaat cgttgattta ttttatagca
    25861 gggatcacta aaagcaaata tatcgattag caacaattgt aaagtttgaa atgtctcaaa
    25921 aggcatattc aactgacgtt gtttgttaat cgatttttct catgttgaaa cttaaaatta
    25981 tgttgaagac tttcatttaa aaaatagaat tttcaatttc tatacgacaa tattcgtatc
    26041 tgtgatatca atgtgatttt aattaaatat gttcaaacaa ttgacgctta ttaaatctaa
    26101 taattagcaa gtttattaca catatattca tttttataca tattttaagc aattgttatg
    26161 cgtaatatga aaaatattta taaatagata accaagatag ataaaccatg tttacccttt
    26221 ttacgtaaag gtgtttagta gtcgtaaatc agtgcatgtg ttaaaataaa tgtataaata
    26281 ttcgtaatca gaggtgttta ctgtgcccaa aaaatcggtt aggcatataa aaattagaga
    26341 aattatttca aatgaacaga tagagacaca agatgaatta gttaaacgat taaacgatta
    26401 tgatttaaat gtcactcaag caactgtttc tcgtgatatt aaagaactac aacttattaa
    26461 agtacctata ccttcaggtc aatatgttta tagtttacca aatgatagaa aattccatcc
    26521 tttagaaaaa ttgggacgtt atttaatgga ttcctttgtt aatatagatg gtactgataa
    26581 tttacttgtt ctaaaaacat tacctggtaa tgcacaatct attggagcta tattagacca
    26641 aatcaattgg gaagaagtac taggcacaat ttgtggtgat gatacttgtt taattatttg
    26701 tcgaagcaaa gaggcaagtg atgaaatcaa gtcaagaatt ttcaatttgt tataaggatg
    26761 cgatgagttt atgttacaaa ccttatcaat caagcaattt gctattattg aagaattaga
    26821 aattcaattt tctgatggtt taactgtttt aagtggtgaa acgggttcag gtaaatcaat
    26881 tattatagat gcaattggac aattaattgg aatgagagct tcatctgatt ttgtaagaca
    26941 tggtgaaaaa aaggctgtca tcgaaggtat atttgatatt gacgagagta aa
//
LOCUS       DAKTGS010000020        26336 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.020, whole genome shotgun sequence.
ACCESSION   DAKTGS010000020 DAKTGS010000000
VERSION     DAKTGS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.020"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(455..901)
                     /gene="perR"
                     /locus_tag="O0286_000734"
     CDS             complement(455..901)
                     /gene="perR"
                     /locus_tag="O0286_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCY0169130.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(998..1948)
                     /locus_tag="O0286_000735"
     CDS             complement(998..1948)
                     /locus_tag="O0286_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0169131.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1954..2409)
                     /gene="bcp"
                     /locus_tag="O0286_000736"
     CDS             complement(1954..2409)
                     /gene="bcp"
                     /locus_tag="O0286_000736"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCY0169132.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2490..3779
                     /locus_tag="O0286_000737"
     CDS             2490..3779
                     /locus_tag="O0286_000737"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0169133.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4072..5166
                     /locus_tag="O0286_000738"
     CDS             4072..5166
                     /locus_tag="O0286_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0169134.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5256..5348)
                     /locus_tag="O0286_000739"
     CDS             complement(5256..5348)
                     /locus_tag="O0286_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169135.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5357..7093)
                     /locus_tag="O0286_000740"
     CDS             complement(5357..7093)
                     /locus_tag="O0286_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCY0169136.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7384..7926)
                     /locus_tag="O0286_000741"
     CDS             complement(7384..7926)
                     /locus_tag="O0286_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0169137.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(8228..9265)
                     /gene="mutY"
                     /locus_tag="O0286_000742"
     CDS             complement(8228..9265)
                     /gene="mutY"
                     /locus_tag="O0286_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCY0169138.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            9417..10394
                     /locus_tag="O0286_000743"
     CDS             9417..10394
                     /locus_tag="O0286_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0169139.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10655..11491)
                     /locus_tag="O0286_000744"
     CDS             complement(10655..11491)
                     /locus_tag="O0286_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169140.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(11500..13017)
                     /locus_tag="O0286_000745"
     CDS             complement(11500..13017)
                     /locus_tag="O0286_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169141.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(13032..13346)
                     /locus_tag="O0286_000746"
     CDS             complement(13032..13346)
                     /locus_tag="O0286_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCY0169142.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(13324..14142)
                     /gene="recX"
                     /locus_tag="O0286_000747"
     CDS             complement(13324..14142)
                     /gene="recX"
                     /locus_tag="O0286_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCY0169143.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(14403..15212)
                     /gene="sgtB"
                     /locus_tag="O0286_000748"
     CDS             complement(14403..15212)
                     /gene="sgtB"
                     /locus_tag="O0286_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCY0169144.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(15553..16068)
                     /locus_tag="O0286_000749"
     CDS             complement(15553..16068)
                     /locus_tag="O0286_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0169145.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(16199..16360)
                     /locus_tag="O0286_000750"
     CDS             complement(16199..16360)
                     /locus_tag="O0286_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169146.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            16584..17735
                     /gene="yfkAB"
                     /locus_tag="O0286_000751"
     CDS             16584..17735
                     /gene="yfkAB"
                     /locus_tag="O0286_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCY0169147.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            complement(17995..18525)
                     /locus_tag="O0286_000752"
     CDS             complement(17995..18525)
                     /locus_tag="O0286_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0169148.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(18615..19871)
                     /locus_tag="O0286_000753"
     CDS             complement(18615..19871)
                     /locus_tag="O0286_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCY0169149.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            complement(19874..20074)
                     /locus_tag="O0286_000754"
     CDS             complement(19874..20074)
                     /locus_tag="O0286_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCY0169150.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            20221..20685
                     /locus_tag="O0286_000755"
     CDS             20221..20685
                     /locus_tag="O0286_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCY0169151.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            20692..20967
                     /locus_tag="O0286_000756"
     CDS             20692..20967
                     /locus_tag="O0286_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0169152.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            21245..22462
                     /locus_tag="O0286_000757"
     CDS             21245..22462
                     /locus_tag="O0286_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCY0169153.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(22578..23207)
                     /gene="vraR"
                     /locus_tag="O0286_000758"
     CDS             complement(22578..23207)
                     /gene="vraR"
                     /locus_tag="O0286_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCY0169154.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(23197..24240)
                     /locus_tag="O0286_000759"
     CDS             complement(23197..24240)
                     /locus_tag="O0286_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0169155.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            complement(24237..24938)
                     /locus_tag="O0286_000760"
     CDS             complement(24237..24938)
                     /locus_tag="O0286_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCY0169156.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            complement(24953..25339)
                     /locus_tag="O0286_000761"
     CDS             complement(24953..25339)
                     /locus_tag="O0286_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169157.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            complement(25556..26314)
                     /gene="map"
                     /locus_tag="O0286_000762"
     CDS             complement(25556..26314)
                     /gene="map"
                     /locus_tag="O0286_000762"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCY0169158.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
BASE COUNT         8562 a         4540 c         3711 g         9523 t
ORIGIN      
        1 aacttaaaac atttgttttt taaatattag tgccgtttga cgacttttat ataatatcaa
       61 gttattctag tagagtcaac ataaaacaac acttattttt gtttaaaaat tactgttgaa
      121 gggattattt tctattcgta agtactatat gtatttaaag tgtaattcct tatgcttttt
      181 acttcagttt tacaaacaaa cttatactca ctttatggat agttatttag tagaccttcg
      241 tttgaatatt tttatgttta tttataaagg atactagtta ttagttaata ccacttccgt
      301 agttagaata ttcttttctt caatttaaat atttttcaga ataagcataa aaaaaagtta
      361 aacaactttc atgtcgttta acttcttaaa tattacgctt acttatatga agtaagctaa
      421 cgctttttta atttgtcata ctaccaaagt taaattattt atcttggcat tctttacaaa
      481 ctccataaat ttccattcga tgatgtgtta cgtcaaagtc agtcatatgc tgagctaatc
      541 tttcaatttc atttaactgt ggatattgaa aatcaacaat cttaccacat tgttcacata
      601 taatatgata atgattatgt gtattaaagt cgaatcgact tgatgagtct ccatatgtta
      661 attcttttac aattccaata tctttaaaca ctcttaagtt attatatatt gtcgcaacac
      721 ttatatttgg aaaatcaggt gaaagtgctt gataaatttc atcagctgtt ggatgagtat
      781 gtgaagaaat taaataacgt aatattgctt gtctttgagg tgtaattctt acgcctgctt
      841 gtcgcaatga tgcaattgat tcttctagtt catgttcaat tgattctatt tcaacactca
      901 tctatatcac catctttctt atataataat tattattact tactataaat ataactgtag
      961 tattgcgcgc gtgtcaatat ttacatgatg atttcattta atagcctttt ttagcatcaa
     1021 cttcattctc aattagacca ttcttattga gaaaattaac tagattgttt ttaaaaatat
     1081 ctaataagtc atactttgct tcataatcat taccagttat atgcgctgtt atagttacat
     1141 tatccaattc atataattca tgattaggtt tcaaaggttc attttcaaac acatctaaat
     1201 atgcatgtcg aataacttta ctttttaata cttctattaa gagcgcttct ttaactatgc
     1261 tacctcgtcc tatatttata aaaagtgctt catctttcat taattcaaaa tgttttttct
     1321 ttagtaaatg aatcgtttct tgcgtttctg gtaaagcatt tataataatg tcagcatttg
     1381 gtaatgtgct ttcaagtgat tcgatagtat atatctcgtc aaactcatct ttgttctgac
     1441 ctgacttgct cagaccaatt aaattcatat taaaagcctt tgctaacttc gcagttctag
     1501 tagcaattgc acctgtacct aaaaataaaa ctgtttgtcc tgataggcgt ttaccagtta
     1561 ttttcgaatc atatatatgt tgtcgttggt tatcatatga tagtttcatc tttttataat
     1621 catctaaaat gaaagctaaa atgtattcag ataattgttt agcttgaaca ccttttccat
     1681 tagttaaaag tatgccgtga tctgcaatat aatctaacgg caatgtattt acacccgttg
     1741 caaaccatgc aatccatttt aaattcgggc aacgtcgtaa aaatgcctca ttgataccgc
     1801 catcataacc aactaatata tctaaatcag ccaagtcgct ctcaggtatt tctgatgctt
     1861 ttttataaaa tttaaaatct aaatctgaaa agcgttgttg taattcagtt tcaacttcac
     1921 gcatacgatt taacccaaca actttcatat atgtcacccc aaaatgtttt taagttcttc
     1981 tatttgtgtt ttaaccttaa ccttctcgat aacatctaat actttacctt gttcatctat
     2041 tataaaagtc gttcttacaa tgcccatact ttctttgcca aatgattttt ttaactgata
     2101 tacgccagtt tctttagcta atttaaaatc ttcatctact aataaatcga aattcaatcc
     2161 gtgtttctca ataaaatttt ggtgtttttt ctttgaatca ccgcttatac catatactgc
     2221 aacatctaaa tcattgaaca tttctaaatt gtctctaaag tcacaagctt ctgtggtaca
     2281 agtaggtgta ttatctctag gataaaaata tataatcgcc tttttacctt ttaatgtatc
     2341 atttgtaatg acagttccgt cttgattttc taatttaaat attggaaatt gttctccttt
     2401 ttgcaacata taatcacctt tttcttcatt ataataattt tatgatacga tattagatga
     2461 aaatattaaa ttaaaagagg ttgatataaa tgaattttag tgaaagtgaa cgtttacaac
     2521 aactttcaaa cgaatatatt ctaggcggtg tcaattcccc ttctcgttct tataaagctg
     2581 taggaggcgg tgcacctgtt gttatgaaag aaggacacgg tgcatattta tatgatgtcg
     2641 atggcaataa atttattgat taccttcaag catacggtcc aattattacg gggcatgcac
     2701 atcctcatat tactaaagca attcaagaac aagctgctaa aggtgtttta tttggtacac
     2761 cgactgaatt agaaattgaa ttcagcaaaa aattacgtga tgcaattcca tctcttgaga
     2821 aaattcgctt tgtaaattct ggaacagaag cagtcatgac aacaattcgt gttgcacgtg
     2881 catatactaa aagaaataaa attataaaat ttgctggatc ttatcatggc cattctgatt
     2941 tagtattggt tgcagcaggt agcggcccat ctcagctcgg ttctccagac tcagctggtg
     3001 ttccagaaag cgtcgcacgt gaagtcatta ctgtaccttt caatgatatt aacgcctata
     3061 aagaagcaat tgaattttgg ggtgatgaaa ttgccgcagt attagtagaa ccaattgttg
     3121 gtaactttgg aatggtaatg cctcaacctg gatttttaga agaggttaat gaaatttcac
     3181 ataacaatgg gaccctagtg atttatgatg aagtaattac tgcattccgt ttccattacg
     3241 gtgccgctca agatttatta ggtgttatcc ctgatttaac tgcatttggt aaaattgttg
     3301 gcggtggttt accaattgga ggctatggtg gacgtcaaga tattatggaa caagtagcac
     3361 ctctaggacc tgcatatcaa gctggtacaa tggctggtaa cccgttatct atgaaagcag
     3421 gtattgcatt actcgaagta ctagaacaag acggtgttta tgaaaaatta gacagcttag
     3481 gccaacaact agaagaaggt ttacttaaat taatcgaaaa acataatatc acagctacaa
     3541 ttaatcgtat ttatggatct ttaacattgt actttacaga tgaaaaagtc acacattatg
     3601 atcaagttga acattctgac ggcgaagcgt tcggtaaatt tttcaaatta atgttaaatc
     3661 aaggtatcaa tttagcacct tctaagtttg aagcttggtt cttaacaact gaacatacag
     3721 aagaagatat taaacaaact ttaaaagctg cagactatgc ttttagtcaa atgaaataac
     3781 ttttaaggaa gtatattttt aattgaatta aaataaagca aatgctcaca tagtagattc
     3841 attttatatc gttaactatt aacgtttaac ttgatatgtc actatgtgag tttttatttt
     3901 acaaatttat actattttaa tctctaacta aagttttaca tcgctatcag tattatgtat
     3961 gatttatttt aacaactata aataagcttg aattttgtaa ctagtaagtg tataacagta
     4021 atgaatacga atattagata ttcaataaac ttaaaagggg cgtcattatt tttgagacta
     4081 ggagctcgga tttttaaaac tggtatagcc attattttag ctatgtctat cgcttcttta
     4141 ctaccggatg atgttggtct gaaagcctta gctggtgtca gtgctgttgt tgcaatgcaa
     4201 cctagtattt atcgttcatt taaaacagtt tctgatcaag cattgggtaa cattataggt
     4261 gccatactat ctgttacaat ggtaacaatt tttagtgata attttatcat tatgggtgtt
     4321 accgtcatag tacttattgc tattttattt aaatttaatt tagcacatgt tgctactttg
     4381 gcgagtgtaa cagcattaat cattatggga caacataccg gttctttcta tattaccgcc
     4441 ttttacaggt ttgtacttgt aatgattggt gtcataagtt catctctagt taactttgtg
     4501 tttctaccac ctaaatttga aacaaaaatt tactataatt cattaaatat atcctcagat
     4561 atttttatgt ggtttaaatt agtattaaat gatacgacgg agtttaataa tattaaacaa
     4621 gatagtcata atttaaaaca acgtgtcgaa aagttagaga aaatttatga ttactatagc
     4681 gaagaacgtc ctattacaaa aaaacatatt caccaacaga atagaaagaa aatacttttc
     4741 agagaagtag ttcagacgac tagacaagct tatgaagtat taaacaaatt gtcgcgttat
     4801 caaaacgact tatatttact taataacaat ttcctattac aaatcaaatt agatttagat
     4861 tcattaactg cttttcacga gcaaatttta gctagccttt ctaaaaaagc acgttataat
     4921 gtgacacatg ttgattatga gttagataat ccgcagaaaa aagatttatt gtccacattt
     4981 cagcatgagt taataaatca tccttatcaa acggaatatt cgtttgccaa tgttatgcaa
     5041 attgttgcag ccatcgaaga gtatcgacac catttagagc atttagaccg cataagaatt
     5101 agctttttca catatcatag atctgatgca gacattgaaa ttgttgaaga agactttgat
     5161 ttataacatt aaaacaattt tatcaattaa tgccacaatg agataaacta atattgaatc
     5221 tcgaaattaa atttaaaaaa gcagtaagat tattttcaat taagaaaatc tcttactgct
     5281 ttttctatgt tatgacccca ccatcctgtt acaaattttc tcttcaagat tgtactgaag
     5341 tcaaacaact aatactttat aagttttgaa tgctatataa atgctcgtaa gcaccttgtt
     5401 ttgcaatcaa ttcacgatgc gtacctgttt caacaatatg tccattttca attacgacaa
     5461 ttttgtcagc atgtgtaata gtggacaagc gatgcgctac gataagtgtc gttcgatctt
     5521 tactcaacac atctaatgct tcttgaataa tggattcact ttctaaatca agtgcacttg
     5581 ttgcttcatc caagataaga attggcggat tatttaaaaa tattctagca atcgataatc
     5641 tttgtttttg accacctgat aatttaacac ctcgttcacc tacttcagtg tcatatccct
     5701 gtggcaagtt cataataaag tcatgtgcat tagccatttt cgccgcttca actacttctt
     5761 catctgttgc tgttggacga ccaagtaaaa tattttcctt aactgtatca gagaataaaa
     5821 tattatcctg ttgcaccaat cctatttgat ttcttaaact tcccgttaaa aaatctttaa
     5881 tgttgtgacc atctattaaa atttgcccag aagttacatc gtaaaatctc ggtattaagt
     5941 taattaatgt tgatttacca ccaccactca tacctacgaa agcaactgtt tctccttttt
     6001 caatactcaa attaatatct tttaaaattg gagcttcgtt atcgttatat tgaaaactaa
     6061 catgatcaat atcaatacga ccttgtttaa tttcaatagg ttgagcacca acaccatttt
     6121 tgatgtcata atcttcatca attaattgga atacacggtc cattgaagca aaactttgcg
     6181 ttaaagttgt aaatgatgcg actaaacgac gtaaaggccc gaacaataac tctaagtatc
     6241 caacaaatgc tgcaagtgta cctactgtga ttgatccaga aatagcaaga tatgcaccaa
     6301 caccgatgac aataattggt ccaatatctg taactgtatt aattgcggca aaggaatagg
     6361 cattccatct tgtatgtttc aacgcacgtg ttaggaaatt agtattcttt ttatcaaagt
     6421 ttttcgcttc attgtcttca atcgcaaaac ttttaacgac tgaaatacct tgaacacgtt
     6481 catgcaagaa tccttgaacc tcagctaatg cttgagatct ttcacgtgtc aattttctta
     6541 atcttccaaa gaaaacgtac accgttaaaa tgtaaaatgg gaagataaac agtgctgcta
     6601 aagtcaattt cacatctaaa aagaacatta tggatagtgc aataataatt gttatacaat
     6661 ctaaccaaat attcattaac ccggttaaaa tgaaatcttt tgtttgttca acatcattaa
     6721 tcactctaga tattacttga cctacttgat tattagcata aaatctcgca cttaaagctt
     6781 gtaaatggtt gtataacttt ttacgtatat catacaatat tttattactt gtccattgcg
     6841 ccaaatattg acgtataaat tcaattggtg gtctcactat tacaaaaata aataatgcga
     6901 taccaatggc aatagttaaa tgatgaactt tttcatcagt cgttagtgcg tggttattaa
     6961 tcacgccatc tattgcatat ttaattaata gtggtataag cattggtata ccaaacttaa
     7021 ttatcccaac aataatcgtt gcaaaaatac gatatttgta tggcttaaca aattgcaaat
     7081 atcgtttaat catacaatcc cccctaatct attgccctat cctattcata agcataaaaa
     7141 tgaatagagg ttggatacat aatttgtaga tgtaaattct tcttacaatt tacattttta
     7201 aaattaagat atctcaacct ctgtcatatt catgtgatta aatacgttgt cttattcgta
     7261 tgttatattc tattcaattt aatctatgga tactgtgtcc ccacacgaca gcaaaagtta
     7321 tcatacttct ttacatcact aagtcaatat aaatgattta atcagtattt acactttatt
     7381 tgcttaatac tgtctaattt ttttgtaacg ttctttccaa actttgataa aatctggcgc
     7441 gaatgggccc ttcttctgtt ctatccattg ttgaagaatg tccacgttgc gtcttaaaat
     7501 aatatcaata tcatgcggat aattcatttg attcatatgt tgctcatatt catcttcatc
     7561 taataaatga tactttccgt ttggatatac tttaatatct aaatcatagt ctatatattt
     7621 taatgcctct tcatcacaaa caaatggtga tgacaaattg caatagtaat aaattccatc
     7681 ttctctaaac atgcagataa cattaaacca atattctgag tgaaagtaaa caattgccgg
     7741 ttcacgtgtt atccaagttc ttccgtcact ttcagtcact aacgtatgat catttccacc
     7801 aatgacaaca tgatcagtac cctttaatat tgttgtttca gaccaaacgc gatgaatctt
     7861 accatcatgt ttataactct gaattttaat gttttcccct tctttaggta tggattctct
     7921 gaccatactc cacaccacct tctgttaatt taaccattat aaattatagc atatttcaga
     7981 aatagtatta tataaataca tatttttacg aaataagatt ttactactta ataattaaac
     8041 tcggtaatat tgctaagtac tacaacagag atttacatgt cccatttaaa gtatataaaa
     8101 tcatcacttt tatatatcaa cactttaact ttttgacatt gttattctat gagatttaaa
     8161 gatatcattt atacttttta aaattaatgt cactatgttt tccgataata ttaccaatca
     8221 tcgaatgtta cccatttata aattgataaa tctttgacat aggtacaggg aatgtatatt
     8281 gatctcgatc acttaaatca aaccaaatca tgtcatctgg taatgtttca atgttaattg
     8341 ctcctgaaac ggcgtatact ttaatcttcc atgttaaatg agtaaattga tgcttcaact
     8401 caaaaatagg tgtttctact ggttgaatgt catgaccgat tttttcagtc attttacgtc
     8461 tagcatgctc actttcaaac ataggaaatt gccacatacc atgcaataat ttttcgctac
     8521 gcttttgcaa cagatattga ccttgattat ttctaattaa aaagacggat tgctcaatta
     8581 cttttttact tacattttta gatttaacag gtaacttttc aaatgtacct ttatcaaatg
     8641 cctcacagtt ttcttgaact ggacaaaata agcataatgg attttttggt gtacaaatta
     8701 acgcccctaa ttccatcata gcttgattaa acgttccagc ttctgtagta acatacggta
     8761 ataattcttg ttcgtacgat ttcctcgtcg attgtaattt aatatctcga tagtcatcat
     8821 tcaatctaga ccatactcga aaaacatttc cgtctacagt tgctagtggt acattatatg
     8881 caatgctcat tactgcagct tgtgtgtatg ggccaacacc ttttaacgct ttaaattgat
     8941 caggatcttt gggaactaag ccttcatatt tatcatgaac ttctttaatc gccgtatgaa
     9001 aatttcgagc tctactataa tatcctaagc cttcccaata ctttaacact tcatcttccg
     9061 aagcttgact caaaacttcc acagttggaa atcgttcaac aaaacgatga taatagtcaa
     9121 taactgtttt aacttgtgtc tgttgtaaca tgacctcact taaccaaata tagtacggat
     9181 tggtcgtttg tcgccatggc atttctcttt gattttcatc aaaccagtgt atcaaatttt
     9241 ctttaaaact agactgctga tacatttata aaaccctttc ctcaccaaaa ttaattgtct
     9301 ttactcataa tgtttttatt gtacattaaa atcatggtta gtatgtaagt taatttagtt
     9361 atttgcgaaa ttggattata atagtatata taatattatg aaatgagtga actgatatgg
     9421 acactgcaac acatatcgca attggggtgg gccttacagc acttgcaact caagatccag
     9481 caatggcttc tacgtttggt gcaacagcta caacccttat cgttggttca ttaattcctg
     9541 atggggatac tgttcttaaa ttaaaggaca atgcaacata tatttcgcat catagaggta
     9601 tcacgcattc catccctttc acaatactat ggccaatttt aattacattt ttaatattca
     9661 cgttctttag tggaaccaac ccatttcatg tatggatgtg ggctcagctc gcagtatttt
     9721 tacatgtctt tgtagatata ttcaattctt atggtacaca agcgcttaga cctatcacaa
     9781 acaaatggat tcaattaagt gtgattaaca catttgaccc tattattttc acagttcttt
     9841 gtattggtat tgtattatgg gttataggct tgcatccatt tgcagtcttc tttcctataa
     9901 tcgctttact aatcatttat tacatgattc gttttaaaat gagagccgta attaagcaac
     9961 aagctttaaa agcaattcaa caagagcatc accctgttaa agtatttgtt gcgccaacaa
    10021 taaaatttat ggaatggcgt gtcgcgatac aaactgatgc acatgactat gttggaaaag
    10081 catatggtag gaatgtggtg tttagtgata aagtggaacg tcaaacatta tcaacagact
    10141 ccattttatg gaaagtcaaa ggtaataaag atatacgtac ttttttaaac ttttcatcaa
    10201 tctatcgttg gcaaacaaca acgttagcag atggttctac tgaaattcgt ttgattgatt
    10261 tgcgttattt aaaaaatgat cattattcat ttgtggcaat tgcacatgta acaaacgata
    10321 atgtcataga ccactcttat attggctggg tatttacaga agataagtta caacgtaaac
    10381 tgtatgctaa ataatttcaa gttattattc actaaagtta atctataaaa aatgaacaac
    10441 cgggcagaat gaaaatcaaa acgattttta ctctgtccgg ttttttaatg taaaactatg
    10501 aatgctttta caaaatctaa aatttatatt gttgctaaca aactaccttt aatgactcga
    10561 aatatcaaaa tcagtatagg aaaacaatat ctagatgata ttctaattgt ttctgattct
    10621 cacagattaa tttacacaac aggtcagcta aacatcatga agaagtatcc gcctcgtctg
    10681 tactatcatt tgaaacatcc tgttgattat cagtttgtgt cactttagat gttttattat
    10741 aaattgcgtg tgtcgtatac cttgctaata cgaaatttac tgcagctatt aaacataaga
    10801 ataaaataaa gtaaaaatga tatggaatgt tttccatact tgatatacca aatatgatag
    10861 attgtgctat accattaaca atgtaataca ttggatttag cattaggatg tgattgataa
    10921 atacatgatt tggatttggt atgaaaataa ttggtaacaa taagaaacac aatacacaaa
    10981 ccccataaaa tatgatattt attttttcag ttaacagtcg aataagacca aaagtaacgg
    11041 atattaatcc tacaaaaata gttgccatca caataaaata gaatagcgct atatatgatg
    11101 tttcgaagtt tactggttta accaatgcac taatcatcgt caaaatgact agcataataa
    11161 aacttaaaat agacataata actactggcg tcgctgcgga aatattaaat aacttagtaa
    11221 ctaaaaagtc attcatataa tgatgataag agatataaat tgtgaacatc actaccgcaa
    11281 aaacaatcaa tacaatgatt ctaaataacc atctcacctc tttaacatcg gtcaaatgac
    11341 taaaggaaaa tagcgtttca cttgcaataa taagtattaa acatgcagct aatacgatta
    11401 gaaaactttt ccaactttgt tttaaacgtt ctgttacata ccttttagta tgcggtaagt
    11461 ttttaaaata taaaataata ttatctagca tctttacacc tacaattcaa tataaatcca
    11521 tttgtcttct ttcaaattgg caatacaata tccattccca actttagtaa tccaaatgtt
    11581 attgttaata ttaaacttat cttttaattc ttttttatct acaattggat aaacaaaacc
    11641 gtataactga ttattatcat tgaaaagtaa ttgtatcggt tgttgtgtaa cggatgcaac
    11701 gaaacggtta tcatcatcag gaactaaagt ttttataact gaactatgct ttttatgtaa
    11761 ttgactatta aagtatgcat agtaattact ataatttgat ttcatatatg gtgctaattt
    11821 tttaaaacta tgcgtttgat ataaacctgc cgggttaaaa tattctaatt tatctttact
    11881 aacatagtaa gtcttattat cggcggtaac acgataattt ttactatttt cgccagtaat
    11941 tgtaactaca ctatattttg gaattgtgac ttgtttatcc ccttgcatat ccacactgcc
    12001 attaaaagca attccataag ctaacttttc gccatatgga tctttatttt cattttgcat
    12061 cgtagcacga ttcgtaaaat ctgttatcga aataattcct atattattga aaatgagcaa
    12121 catcatcaat gctaagccta aaatagtacc tgaggccaaa gtccaaaaac gcactaaaaa
    12181 cttaggcggt tttgcatgat tatagcgctc aacacgtttg aaattatagg tcatctctgg
    12241 tattcttgta cgattctttt tccaatctaa atcaaagttt tcaatttctt cttttgagtt
    12301 tagacttaat cgatcacgtt catgttcttt aaaagatgga ataacttgtt taagtgaccc
    12361 ttccattctt aattgaccat gtgaaaacca agctatgtag ttactcactt gtgaaatttt
    12421 atcaatatca tcaccaattg acacaatcgt taaattattt tcaatataat catttgttaa
    12481 ttcaatcgca cgttccataa attgtggtgt taaatagtca ataacatgat ttaaaataat
    12541 aatatttgat tttgatgagc gtgcaatact taatagtaat tgagcgtatg ccgctttcga
    12601 aatatggtta accggcttcg ttttataatc acctaaatgt gcatattgaa taatctgttc
    12661 agctttatga tcattaattt catatggaaa tagttgaact aactgcgctg tataagcttc
    12721 aacagtttga tgaattaacg attgatcttc aatatatccg tagaacaaat cttcagtaca
    12781 aactacttta cctttatcag gtttaattgc acctgccaac aattgaccta ccaacgcttt
    12841 ggaagattca ggttcaccaa ttatacctaa tgcttctcct tgataaatat gtaaactaat
    12901 attgtttaaa tcgatatctt cagcatcata tccaaaaggt aaataccatt tcttattctg
    12961 tttattccta tagtagtgtg ttacttttag taactttaaa acaattgaac ttcccatcta
    13021 ttttcatcct tctataattg taataacgct ataggtaatc cttcttcggg ttcatcgcta
    13081 ttaaatctaa acccccacgc aaaaatacct tttaatcttt ctactttaaa atattgatta
    13141 ctgccatcag tcagtttata aattttacca atttctatct tttttctgtc tactaagtaa
    13201 ctttcggcaa caattacttt actttgataa acgtcatatt catttaaaat accattcatc
    13261 tcagcttttc tcattttctc tttataaagt tgtatttcgt gtcttaattc tggctcagac
    13321 atctcactca atttcttctg ttccatcggc aataccactt tcttctaatt tagctttaat
    13381 tttatcatat ttatatcctt ttctcataag gccttcgata gtttttgaaa ttaatttctg
    13441 ttgcgtgtac ttctttcgat ttttattata aattttttct aaatctcgtt gtaataaatc
    13501 gtctaaaacc gcttcatctt gtgtaaaatc catttcattc aatacagcat gaatggtttc
    13561 catttcaaac cctttttgaa ttaaagattg cattactttt tctttaactt tattttgtgg
    13621 cccttttttt gttttagata ttttttcagc aatttggata atatcatcaa gttcctgttg
    13681 ttctctataa agttctgtaa atatttcaat gatatttggt tctataccaa gttgatatag
    13741 tttttgttga taaattttag gtcctttatc tgtcgtgcga atcattgtat tttttaaact
    13801 ttccgcataa tcttgatggt cgattaactt ttcgcgataa caatattcaa tcacttcaga
    13861 aatcgcttgc tctgatatct cttctttttg taaatattgt ataacttctt tttcagttct
    13921 ctttttatat gataaatatt ggattgcttt atttaaacct atgcgataat gatcatactt
    13981 ttgaatctct gccatgtcag cagcttcaag ttgttgccct ttttttaaat taaatttgac
    14041 taatgtatca atatctatac ccatttcaaa ttgttcgtct aaaaaaagat taaaccgttc
    14101 tttgtttttc ttttgaactt ctattttagt aatcttcggc actggatcac cccgttactt
    14161 tcttaagttt taatacaata ctcttttatt attcaataag ccacttccta tagcaaatgt
    14221 ttaactttaa atatttttcg atgctaacaa aaaatcacac tatcatcttt taaaatgaaa
    14281 gtgtgattac aagcaaatct gtaaaattta taaagcagaa acaattcaac tttatcatta
    14341 tgacatttca attaaacctt ctacattata gttccaagca tcttacacat gaatgcaagt
    14401 atttaacgat ttaattgtga catagcctgt tgatattgtg tttcattgat ataattttgt
    14461 tgcttcattt tttctaagtt cgtgcttaca cgttgcgtaa aattctctga catattattg
    14521 atattatata cgctaggtgc attgacttta ctagctaaaa tagcgctttg taaaactgtt
    14581 atgtgagaca ttgttgtact atttttattc acggttgttc caaagtaatg gtttgctgcg
    14641 ccctcaagcg tatattgatt atccccaaag taaatattat ttaaataaaa gcttaaaatt
    14701 tcgttcttat tatattgttt ttcaactcga tgagctacaa ataattcttt tacttttcta
    14761 gtaaatgaac gatcattatc ataaaaataa tttttgacaa cttgttgtgt aatggtacta
    14821 ccaccttgca catctctgtc gctaatcgtt gaaaataaag ctctagttgt acctttcaaa
    14881 tcgaatccat gatgattgta gaatcgttca tcttccattg aaataaaggc acctttaaca
    14941 tactctggca tgttatcagc tgacacaaaa ctacttttat tttcaatttt tcttagttca
    15001 tccacattat cgcgtgtaga taaaaaatac atgataccaa taaacaatgc gataatgatt
    15061 agaatggtta ataatatttt taatagtatt cgtttacttt ttttcttttt cggcggtttg
    15121 ccaactggtt gataatacgt attatagtga ggttcgtgtt tcatatgctc aaaatgttca
    15181 tttgagtttg agtacctatc gcttcttttc atgcgtttgc tccttctttt aaaactcact
    15241 tagtatatac cttgagttta ccagtactat cacaaatagg ctacactttt tgggaaaatc
    15301 agtccaaggg cttacaatcg tatacgccat catacttact tttttgtttt ttgaaaaaat
    15361 tatagataaa tcattgcaat tttaaatatt aatcatgtca aatattgtta tattttataa
    15421 aaataaaaga ccatccctat taaatgccaa tagagacgac cttttatttg ttattcattt
    15481 attaaaacta aaatccatat ttcatttcaa acgaaaatat ataaatttta acaatcgata
    15541 accacaatac ttctattgta attgtttaac gatttctcga ttaaaatcat ctaaatcgtc
    15601 tggtactcga cttgttacaa tattgttgtc tacaactact gactcatcaa ctacatgtgc
    15661 gcctgcattt gataaatctt tgcgtacatt taatactgct gttaacgtac gaccttttaa
    15721 atcgtctgta tctattagta tttgtggccc atgacaaatg gcaaatgttg gtacatcatt
    15781 tttagtaaag tatttagcaa atgtgccata tcgaccttct gtatctccac gtaaatgatc
    15841 tggtgaaaat cctccaggaa ttaataatgc atcataatct tctggtttag cttctgcaat
    15901 gcctacatcg acagtaactt tttcaccgtg tttaccaaca acttcactat ttgcagtatc
    15961 tccaatcact acagtattaa agcctgcatt ctctaatgcc tctttagggc ttgaatattc
    16021 tatatcttca aattcgtttg ctagaataat tgctactttt ttagtcattg caaatcacct
    16081 ttctatatat cattgatata attactatag acaagtaaat cagtgattaa acatacaaga
    16141 tataaaaaat attaagcgac tgtcgcgata tctaacccta acacatctta tgtggcattt
    16201 acttagatac taatttaacc ttttcttcaa gctgatctaa caatccaatc cattcatcta
    16261 tatcttcaac acgtacttca tcaggattta catgatcgat atcctcaata aacttattta
    16321 aacgcgcttt tatctgttcg attgtttgtt gttcattcat aaaaagttaa ctccttttat
    16381 tttgttttct ttttcattat tatcctaaca gaaattgcgt taaagcgata taatcttagc
    16441 tatatttatg acattcaaat tattttgact tttaaaaatc cccttttcaa ttaactaaaa
    16501 ttaagagata atttgttacg agtgataata cgaagtggta tcataccgat atgaaccaaa
    16561 tagaaagaag gaagtttaag acgatgaata gcgtcaaatt gaagcaacct gttagcattt
    16621 acaatgatcc atgggaagca tataacgatg ttaaagaaca tggccaatta actttaagta
    16681 acatcgaatt tacaactaca aatctttgta atatgcgttg tagccactgt gcagttggtt
    16741 atactttaca aactgtcgac cccgagcctt tagatatgga cttaatttat cgtagacttg
    16801 atgaaattcc aaatctgcga acgatgtcta ttacaggtgg cgaaccaatg ttttctaaaa
    16861 agtctattag aaatgttgtt aaacctctat taaagtatgc acatcatcga ggtatatata
    16921 cacaaatgaa ttcaaaccta acattgcctc aagatcgtta tttagatatt gctgaatata
    16981 tcgatgttat gcatatctca cacaactggg gaacaactga tgaattcgca aatgttggct
    17041 ttggcgcaat gaagaagcaa ccaccgttaa aagctaagtt aaaattatat gaacaaatga
    17101 tttcgaatgc acgtacatta tcagaacaag gaatgtttgt atctgcggaa acaatgctca
    17161 atcaaagtac gctaccacat ttacgaaaaa tacatcaaga agtcgttcat gatatgaaat
    17221 gtagcagaca cgagattcac cctatgtatc cagctgactt tgcaagtcaa ttaaatgtgt
    17281 taactctagc ggaaatgaaa aagacaattc atgatatatt ggatttcaga gatgaagata
    17341 tttggatgtt atttggtact ttgcctgtgt ttccatgctt aaaggatgat gaagatcaaa
    17401 agttactatc acgtttaaga aatgctaaaa atgtaacgac tagaaatgac ccggatggcc
    17461 gtagtcgttt aaatgtcaat gtatttacag gtaatgtaat cgtaactgat ttcggagatg
    17521 aaacaggtac aatttcgaat atacaaaaag ataaattaac agatgtattt gataaatggt
    17581 tatcctctga tcttgctaaa tcattaaatt gtcattgttc cgagtttagt tgtttaggac
    17641 caaatgttct tgttaaaaat atgtactatc cgaatatgga ttttaaagat aatgagcgtc
    17701 atatgcacaa acaaccacaa attatacaat tttaaaaact cttaattatg cggagaagca
    17761 ctttatcgat aagtagtctc cgcatatttt aatgctatta taaaataaaa aacaattaat
    17821 tgctggcagt actctactta aataataaag ggcatttaat aggactaata gtctataata
    17881 aaaggggtaa attttaacta aaagcataaa cgtgcataat caaaaagaca gattgtaggt
    17941 ggaatattcg aacataacag ttcaattcat ccttaacaat ctgtctttat atttttagtc
    18001 tctaatatgt tgcacttgag ctaaatattc aattgtttgt ttactttcaa tgcgacgtgc
    18061 ttttcttcgt tcaacacgtt gtggtgctgt atcataaaac catttttcaa catcatcttc
    18121 tggatataca ccaggtacat gtttaggttt gccttcatca tctaacgcaa caaatgttaa
    18181 gtaacttaat gcagctaaat catgcttgtt attaaataca tcgtcaattc taatttgaac
    18241 gaccacttcc attgaactag tcccagcgta tgaaaccatc gctacgtatt gtaatatgtc
    18301 ccctgtttta atcggcttta agaaatctac tgagtctgta gatgcggtaa ctacttgcgc
    18361 accagcatgt ttcatagctg tgattgctgc aatttcatca atattagcca tcaatgtacc
    18421 accaaacatt gtatgatggt gattcgtatc ttgagggaaa acttgtctat ttttataaca
    18481 ttttgattct gacatagatt tcattggtct gtcctgattt gtcattattt tatctcccaa
    18541 tctatacttt aatcacgtat ttgaccttta aatacatgtt tcatttccta ctcagcacct
    18601 caaaataaca tttattaaaa tgttgatgcc caatttccat tttcaaatac tagttctttt
    18661 gaaccatctt cgaatatgcc ataaatagtc aaatcactac taccaatcat gaaatcgaca
    18721 tgtacatttg aatcatttaa tccgcttgca atcttttcct caacagtcat ttccgttcca
    18781 ccttgaatat taaaggcgta agcagatcca atcgctaaat gacatgctgc attttcatca
    18841 aataaagtat tgtaaaaaat ggtattacga tttgatatcg gtgaatcatc aggtactaat
    18901 gctacttcac ctaatcttct tgaaccttca tcagtattga ttaaatcttt caataccgct
    18961 tcaccttttc cagctgaaaa atcaataatc tctccatctt taaacatcaa tttaaattga
    19021 tcgataattg tgccattata acttaaaggc aatttattag taacatatcc atctactcta
    19081 tttcgatctg gtgctgtaaa tacttcttca gttggtatat tagcaataaa agcttgttcc
    19141 ttgccgttaa cataacttgt agcatcttcc cagatatgat ttttcgctaa acctactgtt
    19201 aaatcagtac cttcagatac ataatgcaac gcatggtaat ttttttgttg taacttttga
    19261 gcgtaaacac ttaaattagc gatgtgttga cgccaatttt caactggatc gttaccatca
    19321 attcgcacaa tatcgaatac ctcgtcaata aacttaatat atgcttcttc tacagatagt
    19381 tcaggataca ctctcttcgc ccaagcttta gatggaaaag cagctacgac ccatggaaat
    19441 tgattttttt gtacactttc catatatcct ttaaatgcgc gtgcgttttg ttgttgaaat
    19501 gcttgtaatt tttggctatc gataccatcc attaaatcag gatcttcact aataagtgct
    19561 aaattagcag cgccacgttt aacgtaatcc attctcgctt caacatcata tgattttatc
    19621 tcatgattag caaaatgttc cacagattca ttttcaaatt ttaagcgttt taaggttgga
    19681 tcgctatata caacacgtac atctgaagcg ccacaatgat atgcttcttc tacaattaaa
    19741 tgcgttaatt ctaatgtttc tactgatgat ctaataaata ctggctgttt tggttgaaca
    19801 ttcataccaa ccttaactaa tagttcagcg tattgttgta acttttcttt gtaattagtc
    19861 aaaattccca ctcctattcg tgtcgcaatt gtcctaaagc gtcagcaata gctgtcactt
    19921 cacttggcga caatctttct cttgattgaa caaaatcata tatatctgtt aattcttctt
    19981 catttgcatt tttatatttt tcaggatcta ataatccttg attaacaata tttaacttct
    20041 ctctaatagc taatactttc tcttcatttg tcatagccac aatgttcacc tctaataata
    20101 gttatgttat caaagtatca caatttaata aactttaata gaattttata taatctgctt
    20161 tcttattgtt taaagtcgct ataaaatggg gatataacta catagtttgg aggaatcaaa
    20221 atggtagatg tagcatttgt ctgtcttggc aatatatgtc gttctccaat ggcagaagca
    20281 atcatgcgac aaagacttaa agacagaaat attcatgata ttaaagtaca ttcaagaggt
    20341 actggtagct ggaatttagg agagccacct catgaaggta cacaaaaaat tctcaacaaa
    20401 cacaatattc catttgatgg catgattagt gaattattcg aagcgacaga tgattttgat
    20461 tacattgtgg ctatggatca aagtaacgtt gataatatta aatctatcaa tcctaatctt
    20521 aagggacaat tgttcaaact gttagaattt agtaatatgg aagagagtga tgtaccagat
    20581 ccatactaca cgaataattt tgaaggtgta tacgacatgg tattatcatc ttgtgataat
    20641 ttaatagact acatcgtaaa agatgcaaat ttgaaagagg ggtagttttt tatggaaaat
    20701 aaatttgttc ctggtatttt aattggtgcc gtaattggtg gtgcaattag tttagctgat
    20761 aaatctacac gtcaagcttt agttcaatca gttaaagatg caaaaaatgg taaccgcact
    20821 cgtaagcctt ctaaagtcag caagattaaa gacgaagttt tatactggaa agatgttgtt
    20881 gaagaaattc gtcgtaataa tcctgaatta gaacgttcat taaaggatgc gaaagaaaca
    20941 tttgttaata gaaaaaatca acgctaagat gattttgttt ttataaggca atacttaaca
    21001 atttggtgta cgcctttcat agttatcatt attaaatgat aatacaatga ttggccaaac
    21061 tgcattgttt cagtattgcc tttgttttaa ttttaaatca aaatagccta tgaaagattt
    21121 aaatcaatta atttctataa tattatcatt tttaaagcat atcattgttt agttttttta
    21181 taattggata aatactaata gttactttat aaaacattac atagagaaag gttaaggagt
    21241 gcacatgtcg aaaaaggatc actcttcttc aaaatacctt aattctgtta aggaagcgca
    21301 agaggagtca aaaaagaaaa ataaaagtaa tcccaaaatt gatgttgatc gtacatatat
    21361 tgaacctcaa caattccaat ctaagaaacc taaaaaagat gatcaggttt tcttcttatc
    21421 aagattaaat aaacctgcaa aatataagaa agactctaat ttcttatcat atctcatcta
    21481 tcgcatagga aaagatgatg cctcaggact agcagcacaa atgacttacc atttcgtact
    21541 tgctatgttc cctatgttgc ttttcctatt aacattatta ccatttttca atattaagca
    21601 gagtcaaatt actaatatgt taagcaatgc acccgctgaa acatctactc taattaagag
    21661 tgtaattggt gatataactc aaaactccag tggtggctta ttatctatcg gtttgatttt
    21721 agcaatttgg tcagcttcaa atggaatgac tgcaattatg aattctttca atgttgctta
    21781 cgatgtagaa gatagccgta atggaatcgt attaaaacta ctaagtgttg tcttcactgt
    21841 agttatgggc gttgtgtttg tagttgctct agcattacca acgcttggtt ctgtaattag
    21901 tcatttccta ttcggtccac ttggatttga cgaacaagtg aaatggattt ttaaccttat
    21961 tagaattgtg ttaccaatca ttattatatt tatcatattt atcgtgttat attcggttgc
    22021 acctaacgtt aaaacgaagc ttaagtcagt attaccaggt gcagtattta cttcaattat
    22081 ttggttagct ggttcatttg gttttggttg gtatatttca aattttggta actattctaa
    22141 aacatatggc agtatcgcgg gtatcatcat tttgttacta tggttatata tcacaagttt
    22201 tattataatt gtcggtgctg aaatcaatgc aatcattcat cagcgtagtg taattaaagg
    22261 taaaacacct gaagaagcag cattagaaca tgatgacaat aataaaaatc attataatga
    22321 gaacactaga tacgaatatg atgaagatag taatgcaaca catcaacgta cgtatcatgt
    22381 tgatgaacat cctagtgata cctatccaga agatgataaa aatataaaag acaaaatcgt
    22441 tgataagctt aaaaaagact aaacaccaac aaaacagagg ttttcgctaa tgattattgc
    22501 gaaaacctct gttttttggt gtctatttga tgttatattg tgattggcgt aagtaacttt
    22561 tcttaattcg atatgaacta ttgaattaaa ttatgttgga atgcatagat aacagcttgt
    22621 gttctatctt gcacttctaa cttacttaaa atgttactca catgtgtctt aaccgtttta
    22681 atagtaatat gcgatgcact agcaatttct tgatttgagt aacctttcgc aatcaataat
    22741 aatatttcca tttctcgttc tgtaagcatt tcatataact ctgcgcgctt tttcatacgg
    22801 ttacgcattt tcactaaaac ttccggttca aaaacagatt ctcctctaga agttttacga
    22861 actgcatcgg cgatatcttt tgcacttgtt gtttttaaaa tgtaactatc gacacctgca
    22921 tctaatgcac gatatacctc tttatcttca ataaaactag ttaacattaa tactttaatt
    22981 tgcggtaaat cttttttaat ctgagtcgtc gcttctacac catccatgtc atccataagt
    23041 aaatccatta aaattaaatc tggcttcaac tcatgggctt tggcaattgc ttctttacca
    23101 gaagcgcctt caccaactac ttcaatatca ctttgcgttg atagataact tgaaattcct
    23161 atacgtacca tttcatgatc atccacaaac aatactttaa tcgtcatacg aatcctcctt
    23221 atttaaaggt gctttcacct cgatacgtgt acctgaatct ggcaatgata caatatggaa
    23281 cgttgcacca atttccaaag ctctttcacg catattttta agtccataac tttgttctaa
    23341 tttttcatca acattaaaac ctttaccatt atcttgaatt ctcaacaata aataatcgtc
    23401 tttattaaac aattctactg tcacttttgt accgtttgaa tgacgcaatg tattcgaaat
    23461 tgcttcctgt gtaattctga acaaatgatc ttcaatacct ttaggcactt taaaatcttg
    23521 tatttcatgc acaactttca ttggcacttt tttttgtaaa tcaataacta aatctttaat
    23581 accctcacct aaagatttgt cttttaaacc aagcggtctt aaatgtaaca gcaaagcacg
    23641 catttctaac tgcgaatctt gaaccatttt ctctaaaata ggaatttgtt ggtctaatgg
    23701 tggttctaac ttcgtttctt tgatagcaga tagcatcata cttgccgcaa aaagttgctg
    23761 actaacagaa tcgtgaagtt ctcgtgctag tctttgacgt tcatcttcaa taatcttttt
    23821 aactttcaca tcattaatat tataattttc attggttaag ttttgagttt taagtcgcaa
    23881 cttatgcaat tcttgattta aaggtacgag tgtatggtat aaatctaacg tttcactata
    23941 tatttctata ttttgatcat taatgccaac tgtttcgcct tccattgaac gctcaatttg
    24001 cgtcttaatc caatcatttt gctgattgat tttgtaagcg agtaccgaac caacaataat
    24061 acacaataat atgatgatga gatttaaaaa taaaaaaact ggtattccga atatttgtgt
    24121 ataaaacata ccttgaaaat agatgatatt tacaaaaact ttatcgatga acagaaatgc
    24181 agctagcatg ctatatacta agatgagcat tgaaccaatt gttctaatgt agtggttcat
    24241 cgataaatca cctctacgtc tccgataaac gttgatacgt agatattaac tgtatagtta
    24301 tccggtttca tcatttcttc aatatgaata ttgttatttt caactttata tgatttttca
    24361 ttcacgtaag tacttccata aaaagcagct acatgtaaat taatattgta attaaccggc
    24421 aatataacct gcactttacc taaaatgtgt ctaacaacaa tggtattatt ttccttaata
    24481 tttgcagctt ttgttaagtc aatatgtagg tcgccaattc catgttgaat ttgtacatct
    24541 tcccacttat atacataaac tggtgtacgt tgctcaccaa accacttttg tttaataaaa
    24601 gatggtgaag tcataacctc atccgtcgca actacttttt ttggtttaaa cttgtgtatt
    24661 aaataacgca caataagtaa taataaaaag acgaacaaga tgatgattgt atacttatta
    24721 gacaataatg tgaatgcaat taatagcgcg cctatccaaa acgccaaaag gccacgtatt
    24781 ttatgaaaat aaagatatcc tacataaact aatacacatc ccaataatag aacgagtaaa
    24841 aagccaattt tttcaaaaaa tatgtagtaa aaattggcaa taatcattaa tgcagtaaaa
    24901 atgatcaaca tttgcgttga tatatatttg tgtgtcatgt ttcgccgcct ttctatgcca
    24961 cagcgttcat ataatttatt agatacgatg ttttttctgt tgctattttt tcagttgaag
    25021 tttgttgaat ttgagtttca atttgtgcat aatatgcttc aactttattc aactctgatt
    25081 taatattttc aatttcttga ttattgattt ctcttgcaca ttgaatttga aatgcttcag
    25141 caatatcaat atatttattt tctaatgtta gcattagact gtcaattaac ttgctaagtt
    25201 gttctttttt agtaattaaa taacgcatat aatcatctaa atttttcatt ttttcatcta
    25261 gcattaaaag gtagtgcttg atattatttc ttactgttgc tctcaaaaac tgttcaatat
    25321 aacgttcaac atagttcata actatcacct tttataataa gttttaaaat accaaatgcg
    25381 ctatgtatct tcgttgatgt cgatgatatg tttgcttaaa aactactttg ttacaacata
    25441 tttttgaaat gacgcattga ttgtgttctt aattattata aaacagttcc gaaaccaata
    25501 tgaataggct ccggaactaa atgatgttca tactttagtc ttagtatatg ttgaactatt
    25561 cttcttcaat ctttgtcgtt aaaatcggac catccttagt cacgataacc gtatgctcaa
    25621 tttgagcaac aaaactttta tcgctcgttt caaaagccca ttcattttta ccttctgtaa
    25681 caaatgatgc atttgatgag ataaacggtt caatagctaa taccatacct tcagttaata
    25741 atgttttgtc ttttggatca aagtaattaa gtacatgtgc tggtgcttca tgtaatgata
    25801 aaccaacacc atgacctgtt aagtttttaa tgactttcaa atcattttgt ctagctgtat
    25861 tatgcaccgc tttaccaatg ttacttaact tagtacccgg ttttactttt gcaattgcat
    25921 tctcaaatgc catcgttgct acgtcacata ctttttgttt cattggatca tctgattctc
    25981 caacgacaaa tgaaatgcct gtatctgcat aatagccatt cttcaaagcc gatacatcaa
    26041 tatttactaa atctccttca cgaatgacac gcttacttgg aatcccatgt gccacctctt
    26101 cattgacact aatacacgtt tgaccaggaa aattttcatc atgaattggc gcagaaatag
    26161 caccgtattc ttcaaataac tctttcgcaa tattatcaag ctctttcgta gtgatacctg
    26221 gtttggttgc agcttgcatt gtattgcgca ctttagcgca tatgtatcca atttctttta
    26281 acgcttgtaa ttcttcttct gtttttacaa tcattttatc ccgttccttt tttata
//
LOCUS       DAKTGS010000021        26254 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.021, whole genome shotgun sequence.
ACCESSION   DAKTGS010000021 DAKTGS010000000
VERSION     DAKTGS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.021"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            37..885
                     /locus_tag="O0286_000763"
     CDS             37..885
                     /locus_tag="O0286_000763"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCY0169159.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            899..1723
                     /gene="purR"
                     /locus_tag="O0286_000764"
     CDS             899..1723
                     /gene="purR"
                     /locus_tag="O0286_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCY0169160.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            1740..2120
                     /locus_tag="O0286_000765"
     CDS             1740..2120
                     /locus_tag="O0286_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCY0169161.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            2192..2494
                     /gene="spoVG"
                     /locus_tag="O0286_000766"
     CDS             2192..2494
                     /gene="spoVG"
                     /locus_tag="O0286_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCY0169162.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            2684..2815
                     /locus_tag="O0286_000767"
     CDS             2684..2815
                     /locus_tag="O0286_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169163.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            2838..4190
                     /gene="glmU"
                     /locus_tag="O0286_000768"
     CDS             2838..4190
                     /gene="glmU"
                     /locus_tag="O0286_000768"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCY0169164.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            4337..5302
                     /locus_tag="O0286_000769"
     CDS             4337..5302
                     /locus_tag="O0286_000769"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCY0169165.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            5452..6105
                     /locus_tag="O0286_000770"
     CDS             5452..6105
                     /locus_tag="O0286_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCY0169166.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            6416..6988
                     /locus_tag="O0286_000771"
     CDS             6416..6988
                     /locus_tag="O0286_000771"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCY0169167.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            6988..10494
                     /gene="mfd"
                     /locus_tag="O0286_000772"
     CDS             6988..10494
                     /gene="mfd"
                     /locus_tag="O0286_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCY0169168.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            10484..12010
                     /locus_tag="O0286_000773"
     CDS             10484..12010
                     /locus_tag="O0286_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0169169.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            12010..13203
                     /locus_tag="O0286_000774"
     CDS             12010..13203
                     /locus_tag="O0286_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0169170.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            13200..13463
                     /locus_tag="O0286_000775"
     CDS             13200..13463
                     /locus_tag="O0286_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCY0169171.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            13481..13873
                     /locus_tag="O0286_000776"
     CDS             13481..13873
                     /locus_tag="O0286_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCY0169172.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            13978..14379
                     /locus_tag="O0286_000777"
     CDS             13978..14379
                     /locus_tag="O0286_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCY0169173.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            14559..15854
                     /gene="tilS"
                     /locus_tag="O0286_000778"
     CDS             14559..15854
                     /gene="tilS"
                     /locus_tag="O0286_000778"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCY0169174.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            15859..16398
                     /gene="hpt"
                     /locus_tag="O0286_000779"
     CDS             15859..16398
                     /gene="hpt"
                     /locus_tag="O0286_000779"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCY0169175.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            16655..18748
                     /gene="ftsH"
                     /locus_tag="O0286_000780"
     CDS             16655..18748
                     /gene="ftsH"
                     /locus_tag="O0286_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCY0169176.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
     gene            18977..19858
                     /gene="hslO"
                     /locus_tag="O0286_000781"
     CDS             18977..19858
                     /gene="hslO"
                     /locus_tag="O0286_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCY0169177.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            20037..20969
                     /gene="cysK"
                     /locus_tag="O0286_000782"
     CDS             20037..20969
                     /gene="cysK"
                     /locus_tag="O0286_000782"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCY0169178.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            21185..21988
                     /gene="folP"
                     /locus_tag="O0286_000783"
     CDS             21185..21988
                     /gene="folP"
                     /locus_tag="O0286_000783"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCY0169179.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            21966..22331
                     /gene="folB"
                     /locus_tag="O0286_000784"
     CDS             21966..22331
                     /gene="folB"
                     /locus_tag="O0286_000784"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCY0169180.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            22328..22804
                     /gene="folK"
                     /locus_tag="O0286_000785"
     CDS             22328..22804
                     /gene="folK"
                     /locus_tag="O0286_000785"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCY0169181.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            23198..23290
                     /locus_tag="O0286_000786"
     CDS             23198..23290
                     /locus_tag="O0286_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169182.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            23343..24830
                     /gene="lysS"
                     /locus_tag="O0286_000787"
     CDS             23343..24830
                     /gene="lysS"
                     /locus_tag="O0286_000787"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCY0169183.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            25388..25502
                     /gene="rrf"
                     /locus_tag="O0286_000788"
     rRNA            25388..25502
                     /gene="rrf"
                     /locus_tag="O0286_000788"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            25515..25590
                     /locus_tag="O0286_000789"
     tRNA            25515..25590
                     /locus_tag="O0286_000789"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25548..25550,aa:Val,seq:tac)
     gene            25607..25682
                     /locus_tag="O0286_000790"
     tRNA            25607..25682
                     /locus_tag="O0286_000790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25640..25642,aa:Thr,seq:tgt)
     gene            25689..25761
                     /locus_tag="O0286_000791"
     tRNA            25689..25761
                     /locus_tag="O0286_000791"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25722..25724,aa:Lys,seq:ttt)
     gene            25795..25869
                     /locus_tag="O0286_000792"
     tRNA            25795..25869
                     /locus_tag="O0286_000792"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25827..25829,aa:Gly,seq:gcc)
     gene            25877..25965
                     /locus_tag="O0286_000793"
     tRNA            25877..25965
                     /locus_tag="O0286_000793"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25911..25913,aa:Leu,seq:taa)
     gene            25971..26047
                     /locus_tag="O0286_000794"
     tRNA            25971..26047
                     /locus_tag="O0286_000794"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26005..26007,aa:Arg,seq:acg)
     gene            26068..26141
                     /locus_tag="O0286_000795"
     tRNA            26068..26141
                     /locus_tag="O0286_000795"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26102..26104,aa:Pro,seq:tgg)
     gene            26165..26240
                     /locus_tag="O0286_000796"
     tRNA            26165..26240
                     /locus_tag="O0286_000796"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26198..26200,aa:Ala,seq:tgc)
BASE COUNT         9318 a         3598 c         5390 g         7948 t
ORIGIN      
        1 aatcatcata ttaagataac gaaaagaggg cggaaaatga tatatgaaac ggcaccagcc
       61 aaaattaatt ttacgctcga tacacttttt aaaagaaatg atggctatca tgagattgaa
      121 atgataatga caacagttga tttaaatgat cgtttaactt ttcataaaag aaaagatcga
      181 aagatagttg ttgagattga acataattat gtgccttcta atcataaaaa tctcgcatat
      241 cgtgcagcgc aactatttat tgagcaatat caactaaagc aaggtgtaac aatttctatc
      301 gataaagaaa tacctgtttc tgctggctta gctggaggtt cggctgatgc agcagcaacg
      361 ttaagaggat tgaatcgact ttttgatata ggggcgagtt tggaagaatt ggctctacta
      421 ggcagtaaaa tcgggacaga tattccgttt tgtatttata ataaaactgc actatgtact
      481 ggaagaggag agaaaatcga gtttttaaat aaaccacctt cagcttgggt gattcttgct
      541 aaaccaaact taggcatatc atcaccagat atatttaagt tgattaattt agataagcgt
      601 tacgacgtac atacgaaaat gtgttatgag gccttagaaa atcgagatta tcaacaatta
      661 tgtcaaagtt tgtctaatcg attagagcca atttctgttt caaaacaccc acaaatcgat
      721 aaattaaaaa ataatatgtt gaaaagtggt gcagatggtg cgttaatgag tggaagcgga
      781 cctactgtgt atgggctagc acgaaaagaa agccaagcaa aaaatattta taatgcagtt
      841 aacggttgtt gtaatgaagt gtacttagtt agactattag gatagaaggg ttgaaaagat
      901 gagatataaa cgaagcgaga gaattgtttt tatgacgcaa tatttgatga accatccgaa
      961 taaattgatt ccattaactt tttttgtgaa aaaatttaaa caggcgaagt cttcaataag
     1021 tgaagatgtc caaattataa aaaatacatt ccaaaaagaa aagttaggta cagtaattac
     1081 tactgctggc gcaagtggtg gtgttacgta taaaccaatg atgagtaaag aagaggcgac
     1141 tgaagttgtt aatgaggtca ttactctatt agaagagaaa gaacgtttgt tacctggcgg
     1201 atatttattt ttatcagatt tggtaggtaa tccatcgcta ctaaacaaag ttggtaagtt
     1261 aattgccagt atttacatgg aagaaaaatt agatgctgtt gttaccattg cgacaaaagg
     1321 tatttcattg gcaaatgcgg ttgctaatat tttaaattta ccagtagtag tgattagaaa
     1381 agacaacaag gtgactgaag gttctacagt ttcaattaat tacgtttcag gatcttcaag
     1441 aaaaatagaa acaatggtac tttcgaagag aactttagca gaaaattcaa atgttttagt
     1501 tgtcgatgat tttatgaggg ctggtggctc tattaatggt gttatgaatt taatgaatga
     1561 gtttaaagcc catgtaaaag gggtatcagt acttgtagaa tcaaaagaag ttaaacaaag
     1621 attgattgaa gattatactt ccttagtgaa attatctgat gtagatgaat ataatcaaga
     1681 gtttaacgta gaacctggca acagtttatc taagttttca taaaaggagt tttagtatta
     1741 tgaaaatcat taacacaaca agattaccgg aagcacttgg accatattcg catgcaacag
     1801 ttgtgaatgg tatggtttat acttctggtc agattccatt gaatattgat ggacatatcg
     1861 taagcgctga tgttcaagca cagacaaaac aagttttaga aaatttaaag gttgttttgg
     1921 aagaagcagg atctgatttg aattctgttg cgaaagcgac cattttcatt aaagatatga
     1981 atgatttcca aaaaataaat gaagtgtatg gtcaatattt taatgaacac aagccagcgc
     2041 gtagttgtgt agaggttgcg cgtttgccaa aagatgtgaa agtagaaatt gaattagtaa
     2101 gtaaaattaa ggaattataa ttttcgatta atatgtttaa tcaagcttct aaataaaaca
     2161 gagagatata tactataggg gggctcacta catgaaagtg acagatgtaa gacttagaaa
     2221 aatacaaaca gatggacgaa tgaaagcact cgtttccatt acattagatg aagctttcgt
     2281 aattcatgat ttacgtgtaa ttgaaggaaa ctctggcttg ttcgttgcaa tgccaagtaa
     2341 acgtacacca gatggtgaat tccgcgacat cgcgcatcct attaattcag atatgagaca
     2401 agaaattcaa gatgcagtga tgaaagtata tgatgaaaca gatgaagtag taccagataa
     2461 aaacgctaca tcagaagatt cagaagaagc ttaatcaatt ttatatttag cgatgtaata
     2521 catttgcaat aagttgattt gatactgtcg ataaagcata aagctttgtc ggcagttttt
     2581 ttagtttgta ttaatgtttt tttattttta atgaaaggct aataaatata tacgttaaca
     2641 gattatgatg atatgaaaat tattgattca ttgctgataa aaattgcgtt tgaatgatgc
     2701 tcgtattttt gaagtaagaa aaaagttgtt tttaaaatta caacgaatta aaaacaatgc
     2761 cttttatatg ttgaaagagt attgcagatt aaattataat aatgacgaag tgtaaaattt
     2821 aatgggggtt aatgttcatg cgaagacacg cgataatttt ggcagcaggt aaaggcacaa
     2881 gaatgaaatc taaaaagtat aaagtgctac acgaggttgc tgggaaacct atggtcgaac
     2941 atgtattgga aagtgtgaaa ggctctggtg tcgatcaagt tgtaaccatc gtaggacatg
     3001 gtgctgaaag tgtaaaagga catttaggcg agcgttcttt atacagtttt caagaggaac
     3061 aactcggtac tgcgcatgca gtgcaaatgg cgaaatcaca cttagaagac aaggaaggta
     3121 cgacaatcgt tgtatgtggt gacacaccgc tcatcacaaa ggaaacatta gaaacattga
     3181 ttgcgcatca cgaggatgct aatgctcaag caactgtatt atctgcatcg attcaacaac
     3241 catatggata cggaagaatc gttcgaaatg cgtcaggtcg tttagaacgc atagttgaag
     3301 agaaagatgc aacgcaagct gaaaaggata ttaatgaaat tagttcaggt atttttgcgt
     3361 ttaataataa aacgttgttt gaaaaattaa cacaagtgaa aaatgataat gcgcaaggtg
     3421 aatattacct ccctgatgta ttgtcgttaa ttttaaatga tggcggcatc gtagaagtct
     3481 atcgtaccaa tgatgttgaa gaaatcatgg gtgtaaatga tcgtgtaatg cttagtcagg
     3541 ctgagaaggc gatgcaacgt cgtacgaatc attatcacat gctaaatggt gtgacaatca
     3601 tcgatcctga cagcacttat attggtccag acgttacaat tggtagtgat acagtcattg
     3661 aaccaggcgt acgaattaat ggtcgtacag aaattggcga agatgttgtt attggtcagt
     3721 actctgaaat taacaatagt acgattgaaa atggtgcatg tattcaacag tctgttgtta
     3781 atgatgctag cgtaggagcg aatactaagg tcggaccgtt tgcgcaattg agaccaggcg
     3841 cgcaattagg tgcagatgtt aaggttggaa attttgtaga aattaaaaaa gcagatctta
     3901 aagatggtgc caaggtttca catttaagtt atattggcga tgctgtaatt ggcgaacgta
     3961 ctaatattgg ttgcggaacg attacagtta actatgatgg tgaaaataaa tttaaaacta
     4021 tcgtcggcaa agattcattt gtaggttgca atgttaattt agtagcacct gtaacaattg
     4081 gtgatgatgt attggtggca gctggttcca caatcacaga tgacgtacca aatgacagtt
     4141 tagctgtggc aagagcaaga caaacaacaa aagaaggata taggaaataa tcatttacgt
     4201 atttaaaatg gctaggataa aaggataatc ctatgtaata ttaatgtaat ctttatgatt
     4261 taatgattcg catagtaatg gagttacatt ttatatataa tagtaattgc gtaagtaaat
     4321 aattggagga ctataaatgt taaataatga atataagaat tcgtcattaa agattttttc
     4381 attgaaagga aacgaagcat tagcgcaaga agttgctgac caagtaggaa ttgaactagg
     4441 taaatgttca gttaaacgtt ttagtgatgg agaaattcaa attaatatcg aagagagtat
     4501 tcgtggttgt gacgtattta ttattcaacc aacatcatat cctgtgaatc tacatttaat
     4561 ggaattatta attatgattg atgcttgtaa acgtgcttct gcagcaacaa tcaatattgt
     4621 agtgccatat tatggatatg caagacaaga tagaaaagcc cgtagccgtg agccaatcac
     4681 tgctaaatta gttgcaaact taatcgaaac agctggcgca actcgtatga ttgcgttaga
     4741 cttacatgca ccacaaattc aaggattctt tgatattcca attgaccact taatgggtgt
     4801 gccaattctt gctaaacatt tcaaagatga tccgaatatt aacccagaag aatgtgtcgt
     4861 tgtttcacca gaccatggcg gcgttacacg tgcacgtaaa ttagctgaca ttttaaaaac
     4921 tccaattgca attatagata aacgtcgtcc tagaccaaat gttgctgaag tgatgaacat
     4981 tgttggtgag attgaaggac gtacggcaat tattattgac gatattattg atacagcagg
     5041 tacaatcact ttagctgcac aagcattaaa agataaaggt gctaaagaag tatatgcttg
     5101 ttgtacacac cctgttttat caggaccggc taaagaacgt atcgaaaatt ctgctataaa
     5161 agaattaatc gtaacaaact caattcattt agatgaagat cgcaaaccat ctaacactaa
     5221 agaattatct gttgctggtt taatcgcaca agctatcatt cgtgtatacg aaagagaatc
     5281 agttagcgta ttatttgact aatatttaaa aggcgtttga cgaacatatt ccaaacgtgt
     5341 ataatagttt cgttcgtgat tatacgaata aataaacact tgcaagcaac gatgatgttg
     5401 atgggtaagt gaggtgctcg ttttgagcaa aaatgaaagg tggaaatgag aatggcttca
     5461 ttaaagtcaa tcatccgtca aggtaaacaa acacgttcag atcttaaaca attaagaaaa
     5521 tctggtaaag taccagcagt agtatacggt tacggtacta aaaacgtgtc agttaaagtt
     5581 gatgaagtag aattcatcaa agttatccgt gaagtaggtc gtaacggtgt tatcgaatta
     5641 ggcgttggtt ctaaaactat caaagttatg gttgcagact accaattcga tccacttaaa
     5701 aaccaaatta ctcacattga cttcttagca atcaatatga gtgaagaacg tactgttgaa
     5761 gtaccagttc aattagttgg tgaagcagta ggcgctaaag aaggcggcgt agttgaacaa
     5821 ccattattca acttagaagt aactgctact ccagacaata ttccagaagc aatcgaagta
     5881 gacattactg aattaaacat taacgacagc ttaactgttg ctgatgttaa agtaactggc
     5941 gacttcaaaa tcgaaaacga ttcagctgaa tcagtagtaa cagtagttgc tccaactgaa
     6001 gaaccaactg aagaagaaat cgaagctatg gaaggcgaac aacaaactga agaaccagaa
     6061 gttgttggcg aaagcaaaga agacgaagaa aaaactgaag agtaatttta atctgttaca
     6121 ttaaagtttt tatactttgt ttaacaagca ctgtgcttat tttaatataa gcatggtgct
     6181 ttttgtgtta ttataaagct taattaaact ttattacttt gtactaaagt ttaattaatt
     6241 ttagtgagta aaagacatta aactcaacaa tgatacatca taaaaatttt aatgtactcg
     6301 attttaaaat acatacttac taagctaaag aataatgata attgatggca atggcggaaa
     6361 atggatgttg tcattataat aataaatgaa acaattatgt tggaggtaaa cacgcatgaa
     6421 atgtattgta ggtctaggta atataggtaa acgttttgaa cttacaagac ataatatcgg
     6481 ctttgaagtc gttgattata ttttagagaa aaataatttt tcattagata aacaaaagtt
     6541 taaaggtgca tatacaattg aacgaatgaa cggcgataaa gtgttattta tcgaaccaat
     6601 gacaatgatg aatttgtcag gtgaagcagt tgcaccgatt atggattatt acaatgttaa
     6661 tccagaagat ttaattgtct tatatgatga tttagattta gaacaaggac aagttcgctt
     6721 aagacaaaaa ggaagtgcgg gcggtcacaa tggtatgaaa tcaattatta aaatgcttgg
     6781 tacagaccaa tttaaacgta ttcgtattgg tgtgggaaga ccaacgaatg gtatgacggt
     6841 acctgattat gttttacaac gcttttcaaa tgatgaaatg gtaacgatgg aaaaagttat
     6901 cgaacacgca gcacgcgcaa ttgaaaagtt tgttgaaaca tcacgatttg accatgttat
     6961 gaatgaattt aatggtgaag tgaaataatg acaatattga caacgcttat aaaagaagat
     7021 aatcattttc aagaccttaa tcaggtattt ggacaagcaa acacactagt aactggtctt
     7081 tccccgtcag ctaaagtgac gatgattgct gaaaaatatg cacaaagtaa tcaacagtta
     7141 ttattaatta ccaataattt ataccaagca gataaattag aaacagattt acttcaattt
     7201 atagatgctg aagaattgta taagtatcct gtgcaagata ttatgaccga agagttttca
     7261 acacaaagcc ctcaactgat gagtgaacgt attagaactt taactgcgtt agctcaaggt
     7321 aagaaagggt tatttatcgt tcctttaaat ggtttgaaaa agtggttaac tcctgttgaa
     7381 atgtggcaaa atcaccaaat gacattgcgt gttggtgagg atatcgatgt ggaccaattt
     7441 cttaacaaat tagttaatat ggggtacaaa cgggaatccg tggtatcgca tattggtgaa
     7501 ttctcattgc gaggaggtat tatcgatatc tttccgctaa ttggggaacc aatcagaatt
     7561 gagctatttg ataccgaaat tgattctatt cgggattttg atgttgaaac gcagcgttcc
     7621 aaagataatg ttgaagaagt cgatatcaca actgcaagtg attatatcat tactgaagaa
     7681 gtgatcagcc atcttaaaga agagttaaaa actgcatatg aaaatacaag acccaaaata
     7741 gataaatcag tgcgcaatga tttgaaagaa acgtatgaaa gctttaaatt attcgaaagt
     7801 acatactttg atcatcaaat actacgtcgc ttagtagcgt ttatgtatga aacaccttcg
     7861 acaattattg agtatttcca aaaagatgca atcattgcag ttgatgaatt taatcgtatt
     7921 aaagaaactg aagaaagttt aacagtagag tctgattcgt ttattagcaa tattattgaa
     7981 agtggtaatg gatttatagg acaaagtttt ataaaatatg atgattttga aacattgatt
     8041 gaaggctatc ctgtcactta tttttcatta ttcgctacaa caatgccgat aaaactaaat
     8101 catattatta aattttcatg taaacctgtc caacaatttt atgggcaata tgacattatg
     8161 cgttctgaat ttcaacgata tgttaatcaa aactatcata tcgtggtttt ggtcgaaacc
     8221 gaaactaaag ttgaacgtat gcaagcgatg ttaagtgaaa tgcatattcc atcaataaca
     8281 aaattgcatc gctcaatgtc atcggggcaa gcagtgatta ttgaaggcag tttatctgaa
     8341 ggatttgaac tacctgatat gggattagtt gtcattactg agcgtgagct ttttaaatca
     8401 aaacagaaaa agcaacgaaa acgtacgaaa gctatctcaa atgctgaaaa aattaagtct
     8461 taccaagatt taaatgtggg agattatatt gttcatgtgc atcatggtgt tggtagatat
     8521 ttaggtgttg agacgctcga agtggggcaa acgcatcgtg attatattaa attgcaatat
     8581 aaaggtacgg atcaactatt tgttccagta gatcaaatgg atcaagttca aaaatatgta
     8641 gcttcggaag ataagacgcc aaaattaaat aaactcggtg gcagtgaatg gaaaaaaaca
     8701 aaagctaaag ttcaacaaag tgttgaagat attgctgaag agttgattga tttatataaa
     8761 gaaagagaaa tggcagaagg ttatcaatat ggggaagaca cagctgagca aacaacattt
     8821 gaattagatt ttccatatga acttacgcct gaccaagcta aatctatcga tgaaattaaa
     8881 gatgacatgc aaaaatcgcg tccaatggat cgcttgctat gtggtgatgt tggttatggt
     8941 aaaactgaag ttgcagtgag agcagcattc aaagctgtaa tggaaggaaa gcaggttgca
     9001 tttttagttc ctacaactat tttagctcag caacattatg agacgttaat tgagcgtatg
     9061 caagattttc ctgttgaaat tcaattaatg agtcgtttta gaacgcctaa agagataaaa
     9121 caaactaagg aaggacttaa aactggattt gttgacatag ttgttggtac acacaaatta
     9181 cttagtaaag atatacagta taaagattta gggctgttga ttgtagatga agaacaacga
     9241 tttggtgtac gccataaaga gcgtattaaa acattaaaac ataatgtaga tgtactaaca
     9301 ttgactgcaa ccccaatacc tagaacattg catatgagta tgctaggtgt gcgggatttg
     9361 tcagtgattg aaacgccgcc agaaaatcgt ttcccagttc aaacatatgt attagaacag
     9421 aacatgagtt ttatcaaaga agctttagaa agagaactat cccgtgatgg ccaagtgttt
     9481 tatctttata ataaagtgca atccatttat gaaaaacgag aacaactcca gatgttaatg
     9541 ccagatgcta acattgcagt tgctcatgga caaatgacag agcgcgattt agaagaaacg
     9601 atgttaagtt ttatcaataa tgaatatgat attttagtaa cgacgacgat tattgaaaca
     9661 ggtgtcgatg tcccaaatgc aaatactttg atcattgaag atgcagatcg ctttggattg
     9721 agtcagttgt atcaattaag aggtcgtgtt ggtcgttcaa gtcgtattgg ttatgcatac
     9781 ttcttacatc cagcaaataa ggtactaact gagactgcag aagatcgatt acaagcgatt
     9841 aaagaattta cggagttagg ctcaggattt aagattgcga tgcgtgattt gaacattcgt
     9901 ggtgctggta atttgttagg taaacaacag cacggcttta ttgatacagt tggatttgat
     9961 ttgtacagtc aaatgttaga agaagctgta aatgaaaaac gtggtattaa ggaaccagaa
    10021 tctgaggtgc cagaagtcga agttgattta aacttggatg catatttgcc aacagaatat
    10081 attgcaaatg aacaagctaa aattgaaatt tataaaaagc tacgaaaaac tgaaacattt
    10141 gatcaaatta tcgacattaa agatgaatta attgatcgtt tcaatgatta tcctgttgaa
    10201 gtagcacgtt tgcttgatat agtggaaata aaagtacacg cattacattc aggtatcacg
    10261 ttgattaaag ataaagggaa aataattgat attcatttat ctgtaaaagc cactgaaaat
    10321 attgatggcg aagtgctgtt caaagcaaca caacctttag gtagaacaat gaaggttggt
    10381 gttcaaaata atgcaatgac aattacttta acgaaacaaa atcaatggct tgatagtttg
    10441 aagtttttag ttaagtgcat tgaagaaagt atgagaatca gtgatgaagc ataaagaagc
    10501 atttaatggc gttgtcgtgt taactgctgc attaattgtc attaaaattc tgagtgctgt
    10561 atatcgaatt ccatatcaaa atatattagg cgatacaggt ttgtatgcat atcaacaagt
    10621 gtatccaatt gtagcattag gaatgatatt atcgatgaat gccattccta gtgcaattac
    10681 acaaaatata gggaagtatc atagtgacga agcatatgca aaagcagtcg cttatataca
    10741 attagttggt atattattat ttattgctat ttttgtgttt gcgaacaata ttgcacatat
    10801 gatgggtgat ggccatttaa caccaatgat tcaagctgca agtttaagct ttatatttat
    10861 aggtatgctt ggcgtgttaa gaggttatta tcaatctgca aataatatga cagttccggc
    10921 tatttcccag gttatagaac aagttatacg agtaggtatt atcattgtta ctattgttat
    10981 ttttgtagac agaggttgga cgatatatga agcgggaaca attgctattt tagcatcaac
    11041 gataggtttt ttaggttctt caatttattt agtagcgcac cgacctttta agtttaaaat
    11101 ggtaaataac actgcaaaga tcgtttggaa acagttcgca ctttcggttt tgattttcgc
    11161 tatcagtcaa ttaatcgtaa ttttatggca agtgattgat agtgttacta ttattaagtc
    11221 acttcaagcg atacgcgtgc cattcgatgt tgccataact gaaaaaggag tctatgaccg
    11281 tggtgcatca tttattcaga tgggattgat tgtaactaca acatttagtt ttgcgctcat
    11341 tcctctgtta agtgacgcaa ttaaaatgaa taatcaggta cttatgaatc gttatgcaaa
    11401 tgcgtcatta aagattacga ttttaataag tacagcagca ggaataggat taattaattt
    11461 attgccttta atgaacggtg tgttttttaa gacgaatgat ttaaccttaa cgttaagtgt
    11521 ttatatgatt acggtcattt gtgtatcgtt aattatgatg gatatggcat tattacaagc
    11581 gcaacatgct gtgagaccta tttttgttgg tatgacggca ggattggtta ttaaatttat
    11641 acttaatatc attttgattc gtttaagtgg cattattggt gcgagcatta gtactgttgt
    11701 atcattaatt atattcggta cgattatcca tattgctgtc acgagaaaat accacttata
    11761 tgcgatgaga cgatttttta tcaatgttgt tttaggtatg gtatttatgt cgattgttgt
    11821 tcaatgcgtg ttaaacatag tgacaacaca cggtagaatc actggactca ttgaattatt
    11881 atgtgcagca gtattaggta tcattgcatt gtttttctat atttttagat ttaatgtttt
    11941 gacatataaa gagttaactt atttaccatt tggttcaaag ttgtatcaaa ttaagaaagg
    12001 aagacgttga tggcacatac cattacgatt gttggcttag gaaactatgg cattgatgat
    12061 ttgccgctag ggatatataa atttttaaag acacaagata aagtttatgc aagaacgtta
    12121 gatcatccag ttatagaatc attgcaagat gaattaacat ttcagagttt tgaccatgtt
    12181 tatgaagcac ataaccaatt tgaagatgtc tatattgata ttgtggcgca attggttgaa
    12241 gctgctaatg aaaaagatat tgtctatgcg gttccgggtc atcctagagt tgctgagaca
    12301 actacagtga aattactggc tttagcaaag gacaatactg atatagatgt gaaagtttta
    12361 ggtgggaaaa gctttattga tgatgtgttt gaagcagtta atgtagatcc aaatgatggc
    12421 ttcacactgt tagatgcgac atcattacaa gaagtaacac ttaatgttag aacgcataca
    12481 ttgattacgc aagtttatag tgcaatggtt gctgctaatt tgaaaatcac tttaatggaa
    12541 cgatatcctg atgattaccc tgttcaaatt gtcactggtg cacgaagcga tggtgcggat
    12601 aacgttgtga catgcccatt atatgaattg gatcatgatg aaaatgcatt caataatttg
    12661 acgagtgtat tcgtaccaaa aatcataaca tcgacatatt tgtatcatga ctttgatttt
    12721 gcaacggaag tgattgatac tttagttgat gaagataaag gttgtccatg ggataaagtg
    12781 caaacgcatg aaacgctaaa gcgttattta cttgaagaaa catttgaatt gttcgaagct
    12841 attgacaatg aagatgattg gcatatgatt gaagaactag gagatatttt attacaagtg
    12901 ttattgcata ctagtattgg taaaaaagaa gggtatatcg acattaaaga agtgattaca
    12961 agtcttaatg ctaaaatgat tcgtagacac ccacacatat ttggtgatgc caatgctgaa
    13021 actatcgatg acttaaaaga aatttggtct aaggcgaaag atgctgaagg taaacagcca
    13081 agagttatat ttgaaaaagt atttgcagag cattttttaa atttatatga gaagacgaag
    13141 gataagtcat ttgatgaggc cgcgttaaag cagtggctag aaaaagggga gagtaataca
    13201 tgagattaga taaatattta aaagtatcac ggttaataaa gcgacgtacg ctagcaaaag
    13261 aagtaagtga tcaaggtaga attacaataa atggtaatgt tgctaaagct ggatcggatg
    13321 ttaaagttga agatgtgctg acgattcgct ttggtcaaaa attagtaaca gttaaagtaa
    13381 ctgcattaaa tgaacatgca tctaaagata acgcgaaggg tatgtatgaa atcattgaag
    13441 agcgtcgact tgaagaagcg taaattggag gtgacaagca atgaaaaata aagtagaaca
    13501 tatagaaaat cagtacacgt cgcaagagaa caagaaaaaa caacgtcaaa aaatgaaaat
    13561 gcgtgttgtt cgtaggcgta ttacagtatt tgcgggcgta ttacttgcga taattgttgt
    13621 tttatcaatc ttgcttgttg tccaaaaaca tcgcaatgat attgatgcac aggagcgaaa
    13681 agcgaaagaa gcacagtttc aaaagcaaca aaatgaagaa attgcgttaa aagaaaagtt
    13741 gaataatctg aatgacaaag attacattga aaaaattgcg cgtgatgatt attacttaag
    13801 caacaaaggt gaagtgattt ttaggttgcc agaagacaaa gattcgtcta gctcaaaatc
    13861 ttcgaaaaaa taaatccaaa ttgattcaaa attatccgag tatagacatt gtgaaaaaat
    13921 ccaaacaagg atataataag ggaaaatcga atcaaatcgg gaggatttat ttaacatatg
    13981 tcaatcgaag ttggaaataa gcttaaaggt aaagtcactg gtattaaaaa gtttggtgca
    14041 ttcgtagaat tacctgaagg aaaaagtggt ttagttcaca ttagtgaagt cgcagataat
    14101 tatgttgaaa acgtagaaga gcacctttct gttggtgatg aagtagacgt aaaagtatta
    14161 tctattgctg atgatggaaa aattagtctt tcaattaaga aagctaaaga ccgtccacgt
    14221 agacaacata cgagtaaacc aagtcatcaa aaaccagtgc aaaaagccga agattttgaa
    14281 aagaaattaa gcaatttctt aaaagatagt gaagataaat taacttcaat caaacgtcaa
    14341 acagaatcta gacgcggtgg caaaggttca agacgttaat taaaataaat aaagactgct
    14401 tcgataagga atatatttag aatgatgcgt atcgaataat cgattgcagc gttagacaat
    14461 ctaagactgt ttcttaaata aggagcagtc tcttttattt gtaatgatat aactaagact
    14521 tataccattt ttgaaaattg taaaagtgag gtgatgttat gcagttaaat agtaatggtt
    14581 ggcatgttga tgaccatatt gttgtcgctg tttctacagg tattgatagt atgtgtttat
    14641 tgtatcaact actaaatgat tataaagata gttatagaaa actaacatgc ttacatgtca
    14701 atcatggcgt taggtcagct tcaattgagg aagccagatt tttagaagca tactgcgaac
    14761 gtcatcacat cgatttacat atcaaaaagt tagatttgtc gcatagtctc gaccgaaata
    14821 acagcattca gaatgaagct cgaattaaac gttacgaatg gtttgatgaa atgatgaatg
    14881 tattagaagc ggatgtattg ctaacggcgc atcatttgga cgatcaatta gaaactatta
    14941 tgtatcgtat ttttaatggg aaatcaacac gtaataaact aggatttgat gagttatcga
    15001 agcgaaaagg ttatcagatt tatcgaccac ttttagctgt ctctaaaaaa gaaataaaac
    15061 aattccaaga gagatatcat attccatatt ttgaagatga atctaataaa gataacaaat
    15121 atgttagaaa tgatattcgt aatagaatta ttccagctat tgatgaaaat aatcaactta
    15181 aagtatcgca tttattaaaa ttaaaacaat ggcatgatga acaatatgat attttgcaat
    15241 attcagctaa acaatttatt caagaatttg tgaagtttga tgaacagtca aaatatttag
    15301 aggtttctag acaagctttt aataacttac caaactcatt aaagatggtt gtgttggact
    15361 gcctattatc aaagtattat gagttgttta atattagtgc taaaacatac gaagagtggt
    15421 ttaaacaatt tagtagtaag aaagcacaat tcagtattaa tctcacggat aaatggataa
    15481 ttcaaatcgc atatggtaaa ttaataataa tggctaaaaa taatggcgat acatatttta
    15541 gagttcaaac aattaaaaag ccaggtaatt atatttttaa caaatatcga ttagagatac
    15601 attctaattt accaaaatgt ttatttccgc ttacagtgag aacacgacaa agtggcgata
    15661 catttaaact gaatgggcgc gatggttata agaaagtgaa tcgcctgttt atagattgta
    15721 aagtgccaca gtgggttcgg gatcaaatgc caatcgtatt ggataaacaa cagcgcatta
    15781 ttgcggtagg agatttatat caacaacaaa caataaaaaa atggattata attagtaaaa
    15841 atggagatga atagcgttat gcataatgat ttgaaagaag tattgttaac tgaagaagat
    15901 attcaaaata tctgtaagga attgggagca caattaacaa aggattatca aggtaaacca
    15961 ttagtatgcg tgggtatctt aaaaggctca gcaatgttta tgtcagattt aattaaacga
    16021 attgataccc atttatcaat tgatttcatg gatgtttcta gttatcacgg aggcactgag
    16081 tcaactggtg aagttcaaat cattaaagat ttaggttctt ctattgaaaa taaagacgta
    16141 ttaattattg aagatatctt agagactggt actacactta agtcaattac tgaattatta
    16201 caatctagaa aagttaattc attagaaata gttactttat tagataaacc aaaccgtcgt
    16261 aaagcggaca ttgaagctaa gtatgtaggt aaaaaaatac cagatgaatt tgttgttggt
    16321 tacggtttag attatcgtga attataccga aacttaccat atatcggtac gttaaaacct
    16381 gaagtgtatt caaattaatt ttttaatcaa tttcagttat tattactatg cgtttgagaa
    16441 ataatagtgt agactcaaaa atatgaaaaa tgtatttcat atatatttaa ttttagacaa
    16501 gacatatgtc ttgaaaagtt gaaaaatata gagattgata aaactaatac gggtgtgaat
    16561 gacattgatg ttaagctcaa ttactagctt ataaaacatg tcatatgtta caatttttgt
    16621 tagttttatt atgggaagta ggaggaaatg acgcatgcag aaagcttttc gcaatgtgct
    16681 agttatcgta ataataggcg ttattatttt tggtctattt tcatatttaa acggtaatgg
    16741 aaatatgccg aaacagctta catataatca atttactgag aagttggaaa aaggtgacct
    16801 taaaacttta gaaatccaac cacaacaaaa tgtctatatg gtaagtggta aaacgaaaaa
    16861 tgatgaagac tattcatcaa ctattttata taacaacgaa aaagaattac aaaaaattac
    16921 tgatgctgct aaaaagcaaa acggtgtaaa attaacgatt aaagaagaag aaaaacaaag
    16981 tgtctttgtg agtatacttt caacattaat tccagttgta gtcatagcgt tattatttat
    17041 tttcttccta agccaagcac aaggtggcgg tagtggcggt cgtatgatga actttggtaa
    17101 atctaaagca aaaatgtacg ataataataa acgtcgtgtt cgtttctctg atgtagcagg
    17161 ggcagatgaa gaaaaacaag aattaattga aattgttgat ttcttgaaag ataataaaaa
    17221 attcaaagaa atgggatcta ggattcctaa aggtgtctta cttgttggac ctccaggtac
    17281 tggtaaaaca ttacttgcta gagcggttgc aggtgaagct ggcgcaccat tcttctctat
    17341 tagtggttca gactttgtag agatgtttgt tggtgttggt gcgagccgtg ttcgtgactt
    17401 attcgataat gctaagaaaa acgcgccttg tatcatcttt atcgatgaga ttgatgctgt
    17461 tggtcgtcaa cgtggtgcag gtgttggtgg cggtcatgat gaacgtgaac aaaccctaaa
    17521 ccaattatta gttgaaatgg atggtttcgg tgaaaatgaa ggtatcatta tgatagctgc
    17581 tacaaaccgt cctgatatcc ttgacccagc cttattacgt ccaggtcgtt ttgatagaca
    17641 aattcaagtt ggtcgtccag atgtgaaagg ccgtgaagca attcttcatg ttcatgctaa
    17701 aaacaaacca cttgatgaaa cggttgattt aaaagcaatt tcacaacgta cacctggttt
    17761 ctcaggtgct gatttagaga acttattaaa tgaagcatct ttaattgctg tacgtgaagg
    17821 taaaaagaaa attgacatga gagatatcga agaggcaacg gatagagtta tagccggacc
    17881 tgctaagaaa tctcgagtta tttctaagaa agaacgtaat attgttgctc atcacgaagc
    17941 tggtcataca attatcggta tggtacttga tgaggcagaa gtagtgcata aagttactat
    18001 tgttccacgt ggacaagcag gtggttatgc aatgatgcta cctaaacaag atcgtttctt
    18061 aatgactgaa caagagttat tagataaaat ctgtggttta cttggtggac gtgtatcaga
    18121 agatattaac tttaacgaag tatcaacagg tgcttcaaat gacttcgaac gtgcaacaca
    18181 aatcgcacgc tcaatggtta cgcaatatgg tatgagtaaa aaattaggac cattacagtt
    18241 cggtcatagc aatggtcaag tattcttagg taaagatatg caaggtgagc ctaattattc
    18301 aagccaaatc gcatatgaaa ttgataaaga agttcaacga atcgttaaag aacaatacga
    18361 acgttgtaaa caaattttat tagagcacaa agaacaatta attttaattg ctgaaacatt
    18421 attaacagaa gaaacattag ttgctgaaca aattcaatca ttattctacg aaggtaaatt
    18481 acctgaaatt gattatgatg cagctaaagt tgttaaagat gaagattctg aatttaatga
    18541 tggtaaattc ggtaaatctt atgaagagat tcgtaaagag caattagaag atggacaacg
    18601 tgacgaaagt gaagatcgta aagaagaaaa agatattgct gaggataaaa aagaagctga
    18661 taaatctgat gaaaaagatg aaccagcaca tcaacaagcc ccaaatatcg aaaaacctta
    18721 cgatccaaat cacccagaca ataaataatc gattatattc agtacctctt tctatgataa
    18781 agttatagaa agaggtactt ttatcgtttt tgaaaatacg tattagattt taagtcgttg
    18841 aattgttata gcagaaaata attgtaaaac aagttacttc attatttaga atgatgggtg
    18901 tagaataagt acaattgttg cattttatga agtaaagtaa ttttttaaat atagagtaat
    18961 agaggagatt gaaataatga cacacgatta tattgttaaa gcattagcat ttgatggaga
    19021 gattagggct tatgctgctt tgacaactga aactgttcaa gaagcacaaa cgagacatta
    19081 tacatggccg acagcatctg ctgcaatggg aagaacaatg acagcaacag ctatgatggg
    19141 cgcaatgttg aaaggtgatc aaaaattaac tgtcactgta gatggccaag gacctattgg
    19201 acgaattatt gccgatgcaa atgctaaagg cgaggtgcgt gcttatgtag accatccaca
    19261 aactcatttt ccattaaatg agcaaggtaa acttgatgta agacgagcgg tagggacaaa
    19321 tggatctatt atggttgtta aagacgttgg aatgaaagac tatttctctg gagcaagtcc
    19381 aattgtttca ggagaacttg gtgaagattt tacttattat tatgctacaa gtgaacaaac
    19441 accttcatcg gtaggtcttg gtgtattggt aaatcctgat aatacgatta aagcagcagg
    19501 aggatttatc attcaagtta tgccaggtgc caaagatgaa acaatttcaa aattagaaaa
    19561 agcaattagt gaaatgacac cagtttctaa attaattgaa caaggattaa cgccagaagg
    19621 attactaaac gaaatcttag gtgaagacca tgtgcaaatt ttagagaaaa tgcctgttca
    19681 atttgaatgt aattgtagtc atgagaaatt tttaaatgct attaaaggat tgggcgaggc
    19741 tgagattcaa aatatgatta aagaagatca tggtgctgaa gcagtatgtc atttctgtgg
    19801 aaataaatat aaatatactg aagaagaatt aaacgtgttg ctagaaagtt tagcgtaatt
    19861 taatttaaat caatacgcta aaatgtttat ttttagcggt ttagtgaaat gtagaactaa
    19921 atagttgtat aatccttagt gattttgttt gctttctaga atttatttga taaaataatt
    19981 ctatatccga taaataaact aagatttcaa caactaacta aaaaggagtg ttcttaatgg
    20041 cacaaaaacc agtagataat attactcaaa ttattggcgg tacaccggta gtcaaattga
    20101 gaaatgtagt agatgacaat gcagcagatg tttatgtaaa attggaatat caaaatccag
    20161 gtggttctgt aaaggataga attgctttag caatgattga aaaagcagag cgagaaggca
    20221 aaattaaacc tggcgataca attgtagaac caacaagtgg taatacaggt atcggtttag
    20281 catttgtatg tgctgctaaa ggatataaag cagtatttac tatgcccgaa acaatgagcc
    20341 aagagcgtcg taatttatta aaagcatacg gtgcggaatt agttttaacg cctggatcag
    20401 aagcgatgaa aggtgcaatt aaaaaagcta aagaattgaa agaagaacat ggttacttcg
    20461 agccacaaca atttgaaaac cctgcgaacc ctgaagttca tgagttaact acaggtcctg
    20521 agttattaca acaatttgaa gggaaaacta tcgatgcgtt cctagctggt gttggtactg
    20581 gtggtacgtt atctggtgta ggtaaagttc tgaaaaaaga atatcctaac atcgaaattg
    20641 ttgctataga gcctgaggct tctccagtat tgagcggtgg tgagccaggt ccacataaat
    20701 tacaaggttt aggtgctgga tttattccag gcactttgaa tacagaaatc tatgacagta
    20761 ttattaaagt aggaaatgat acagcgatgg aaatgtctcg tcgagttgct aaagaggaag
    20821 gtattttagc aggtatttca tcaggtgctg cgatttatgc tgccattcaa aaagcaaaag
    20881 aattaggaaa aggtaaaaca gtagtaacag tattgccgag taatggtgaa cgctacttat
    20941 caacaccttt atattcattc gatgactaat taatgtcatt taaaagagtg agttatcttt
    21001 ttgagataac ttgctctttt tttctaccat gtatattttt aaaaatatga gcgttaaatt
    21061 aaacattttt ctgataaaaa tatccagtga atgataagat aataaacgta catactaata
    21121 actagtaaat agcaggagta aattttatta gagttaaaca atacataatt aaagggtggt
    21181 taacatgact aaaacaaaaa ttatgggcat attaaacgtc acacctgatt cattctcaga
    21241 tggtggaaaa tttaataatg ttgaatcagc tataaataga gtgaaagcca tgatagatga
    21301 aggtgctgac attatagatg ttggaggtgt ttcaacgaga cccggtcatg aaatggtttc
    21361 attagaagaa gagatgaaca gagtattacc tgttgttgaa gctattgtcg gttttgatgt
    21421 aaaaatttca gtcgatacat ttcgaagtga ggttgctgaa gcatgtttaa aattaggcgt
    21481 tgatatgatt aatgatcaat gggcgggtct gtatgatcat cgtatgttcc aaattgtagc
    21541 taaatatgac gcggaaatta ttttaatgca taatggaaat ggtaatcgtg atgaaccggt
    21601 tgtcgaagaa atgttaacat ctttgttagc acaagcacat caagctaaaa tagctggtat
    21661 accttcaaat aaaatttggc tagatccagg tataggtttc gctaaaacta gaaatgaaga
    21721 agccgaagtt atggcaagac tggatgaact tgttgcaaca gaatatccag ttttattagc
    21781 gacaagccgg aaacgtttca ctaaagagat gatgggttat gatacaacac cggttgaaag
    21841 agatgaagta actgcagcta cgactgcata tggtattatg aaaggcgtta gagcagtacg
    21901 cgttcataat gtcgagttga atgctaaatt agctaaaggt atagattttt taaaggagaa
    21961 tgaaaatgca agacacaatc tttcttaaag gtatgcgctt ttatggatat catggtgctt
    22021 tatcagctga aaatgaaata gggcaaattt tcaaagtgga tgtaactttg aaagtagact
    22081 tagctgaagc tgggcgtact gataatgtta ttgatacagt tcattatggt gaagtgttcg
    22141 aagaggttaa atcaattatg gaaggtaagg ccgttaattt acttgagcat ctagctgaac
    22201 gtattgcaaa tcgtataaat tcacaatata atcgtgtaat ggaaacgaaa gtgagaatca
    22261 ctaaagaaaa cccaccgatt ccgggtcatt atgatggagt aggtatcgaa atagtgaggg
    22321 agaataaatg attcaagcat acttaggatt aggtagtaat attggtgata gagaaagcca
    22381 gttaaacgat gctataaaga ttttgaatga atatgatggt attaacgtat ctaatatttc
    22441 tccgatttat gaaacagcac cagttgggta tactgagcaa cctaactttt taaatttgtg
    22501 tgttgaaatt caaacaacac tcacagtatt acaactgttg gaatgttgtt tgaagacaga
    22561 agaatgttta caccgtatta gaaaggaacg atggggtcct agaactttag atgtggatat
    22621 tttgttgtat ggagaagaaa tgatagattt accaaaactg tcggtgccac atccgagaat
    22681 gaatgaacgt gcatttgttt taatcccatt aaatgatata gcagcaaatg tcgtagaacc
    22741 acgttcgaaa ttgaaagtga aagatttagt ttttgtcgat gacagtgtaa agagatataa
    22801 ataatgtatt gttgagaaca ttcatattta ttgggaatag attattgatt attggagatg
    22861 tttccgcatt ttgattatct ctaaatgcat ttgatttcga attagtatat gatgatttag
    22921 tatgtgttag aattaagtta tttcataaaa tcaaagtaag caatatttga taaattgctg
    22981 taatttattt aggcttttgt tgaagtttgt ggaagataat ttttatacat ttacttaggg
    23041 ttgtattaat gataatcgta ttttaaatga ataagtaatt cataaacgaa aacagaaatt
    23101 gctttattac atttacaagc tcttgtaaaa ttaaaaaagt gataaaattg atgttatgaa
    23161 tataagaaaa catgccacat cataacttta aggtgtaatg gttaatgata aagtattaga
    23221 aacatcgaaa gagatgtatg ttgagcaaaa atgtctgata ttttataaaa ctttaaagga
    23281 aaatgtttga gtgtaccagt tggaatacta aaggattaca acaagttaaa ggagagaaag
    23341 ttatgtcaga agaaatgaat gaccaaatgt tggttcgacg tcaaaaatta caagaattat
    23401 atgatcttgg tatagacccg tttggttcta aatttgaccg ttcaggttta tctagtgatt
    23461 tgaaagaaga gtgggaccag tattctaaag aagaattggt agaaaaagaa gcggatagtc
    23521 atgtcgctat agctggacga ttaatgacta agcgtggtaa aggtaaagca ggatttgcac
    23581 acgttcagga cttagctgga caaattcaaa tttacgttcg taaagatcaa gttggcgatg
    23641 acgaatttga tttatggaaa aatgctgatt taggcgatat cgttggtgtt gaaggtgtaa
    23701 tgttcaaaac aaatactggc gaattatcgg ttaaagcgaa gaaattcacg ctactaacta
    23761 aatcattgcg accattaccg gataaattcc acggtttaca ggatattgaa cagagatatc
    23821 gtcaaagata tttagattta attacgaacg aagatagcac tcgtacattt attaatcgta
    23881 gtaaaatcat tcaagaaatg cgtaattatt taaataataa aggtttcttg gaagtagaaa
    23941 cacctatgat gcaccaaatt gctggtggag cagctgctag accatttgta acacatcata
    24001 atgcattaga tgcaacgtta tacatgcgta ttgctattga gttgcattta aaacgtttaa
    24061 ttgtcggtgg acttgaaaaa gtatatgaaa ttggtagagt attccgtaat gaaggtgtat
    24121 caactagaca taaccctgaa ttcacaatga ttgaattata tgaagcatat gcagattatc
    24181 atgacattat ggatttaaca gaatctatgg tgagacatat tgccaatgaa gtgttaggtt
    24241 ctgcaaaagt acaatacaat ggggaaacga tcgatttaga atctgcttgg actcgtttgc
    24301 atattgttga tgctgtaaaa gaagctactg gtgtagattt ttatgaagtt aaaagtgatg
    24361 aagaagctaa agctttagct aaagaacatg gtattgaaat taaagataca atgaaatatg
    24421 gtcatatttt aaatgaattc tttgagcaaa aagttgaaga aacacttatt cagccaacgt
    24481 ttatctatgg tcatccgact gaaatttcac ctttagcgaa gaaaaatcct gaagatccta
    24541 gatttactga tcgtttcgaa ttgttcattg taggtagaga gcatgcaaat gcatttactg
    24601 aattaaatga tcctattgat caaaaaggtc gttttgaagc gcaacttgtt gaaaaagcgc
    24661 aaggtaatga tgaagcgcat gaaatggatg aagattacat tgaagcgtta gaatatggta
    24721 tgcctccgac aggtggtctt ggtatcggta ttgacagatt ggttatgtta ttaactgact
    24781 ctccatcaat cagagacgta ttattattcc cttatatgag acaaaaataa atgacgttga
    24841 ttgttagtaa gagctctcgt gtatacaaca tgtgtatgcg agggctttct taattatggt
    24901 aattagttcg tgtttgaatg tttttgatag taatagttaa cgatagtggt gctatttttg
    24961 actgttaaac aaggtagttg gctgatagat gaaaatgacg atacgtatat agagtagttc
    25021 ggatgagaaa tgttaacgat agacattgag atatcttata acaaaaattg ctatattagt
    25081 ataatttatc ttaatcagct ataaaagtac tttaaaattg tatagaatgt gtatggtttt
    25141 gtacatatgt gtatgataga atactaaaag tgttatgaat tagagggcac gagaaatgtc
    25201 agttttgaag aataaaaaag ttgattaaaa gtgttgactt tatcaattga atgaagtaat
    25261 atataaaagt cgccaaaaac agacgaaaca cactaaaagc tgatgtgaca aagtttacat
    25321 caaagtgtaa aattaactat tgcaccttat taattaagcg tgtatcatga ataagtaagt
    25381 tattttgtct ggtgactata gcaaggaggt cacacctgtt cccatgccga acacagaagt
    25441 taagctcctt agcgtcgatg gtagtcgaac ttacgttccg ccagagtaga acgttgccag
    25501 gcaaatgaca aatcggagaa ttagctcagc tgggagagca tctgccttac aagcagaggg
    25561 tcggcggttc gaacccgtca ttctccacca tttattctta gatatagccg gcctagctca
    25621 attggtagag caactgactt gtaatcagta ggttgggggt tcaagtcctc tggccggcac
    25681 catcttttga gccattagct cagctggtag agcatctgac ttttaatcag agggtcagag
    25741 gttcgaatcc tctatggctc attacgattt aatttttata tttagcaaaa taatgcagaa
    25801 gtagttcagc ggtagaatac aaccttgcca aggttggggt cgcgggttcg aatcccgtct
    25861 tctgctccat tattttgccg gggtggcgga actggcagac gcacaggact taaaatcctg
    25921 cggtgagaga tcaccgtacc ggttcgattc cggtcctcgg caccatttta gcgcccgtag
    25981 ctcaattgga tagagcgttt gactacggat caagaggtta tgggttcgac tcctatcggg
    26041 cgcgccattt ttaaattaat tgaataacgg gaagtagctc agcttggtag agcacttggt
    26101 ttgggaccaa ggggtcgcag gttcgaatcc tgtcttcccg attacttctt aaattccatt
    26161 ttatgggggc ttagctcagc tgggagagcg cctgctttgc acgcaggagg tcagcggttc
    26221 gatcccgcta gtctccacca tttatttttt acac
//
LOCUS       DAKTGS010000022        26092 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.022, whole genome shotgun sequence.
ACCESSION   DAKTGS010000022 DAKTGS010000000
VERSION     DAKTGS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.022"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(24..191)
                     /locus_tag="O0286_000797"
     CDS             complement(24..191)
                     /locus_tag="O0286_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169184.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(319..822)
                     /locus_tag="O0286_000798"
     CDS             complement(319..822)
                     /locus_tag="O0286_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0169185.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(815..1402)
                     /locus_tag="O0286_000799"
     CDS             complement(815..1402)
                     /locus_tag="O0286_000799"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCY0169186.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1414..2214)
                     /gene="racE"
                     /locus_tag="O0286_000800"
     CDS             complement(1414..2214)
                     /gene="racE"
                     /locus_tag="O0286_000800"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCY0169187.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2450..3265)
                     /gene="sdhB"
                     /locus_tag="O0286_000801"
     CDS             complement(2450..3265)
                     /gene="sdhB"
                     /locus_tag="O0286_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCY0169188.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3265..5031)
                     /gene="sdhA"
                     /locus_tag="O0286_000802"
     CDS             complement(3265..5031)
                     /gene="sdhA"
                     /locus_tag="O0286_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCY0169189.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5083..5697)
                     /locus_tag="O0286_000803"
     CDS             complement(5083..5697)
                     /locus_tag="O0286_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCY0169190.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6021..7802)
                     /gene="uvrC"
                     /locus_tag="O0286_000804"
     CDS             complement(6021..7802)
                     /gene="uvrC"
                     /locus_tag="O0286_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCY0169191.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7968..8063
                     /locus_tag="O0286_000805"
     CDS             7968..8063
                     /locus_tag="O0286_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169192.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8126..8440)
                     /gene="trxA"
                     /locus_tag="O0286_000806"
     CDS             complement(8126..8440)
                     /gene="trxA"
                     /locus_tag="O0286_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCY0169193.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8613..10961)
                     /locus_tag="O0286_000807"
     CDS             complement(8613..10961)
                     /locus_tag="O0286_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCY0169194.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10971..12683)
                     /gene="polX"
                     /locus_tag="O0286_000808"
     CDS             complement(10971..12683)
                     /gene="polX"
                     /locus_tag="O0286_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCY0169195.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(12756..13277)
                     /locus_tag="O0286_000809"
     CDS             complement(12756..13277)
                     /locus_tag="O0286_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCY0169196.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13278..13544)
                     /gene="zapA"
                     /locus_tag="O0286_000810"
     CDS             complement(13278..13544)
                     /gene="zapA"
                     /locus_tag="O0286_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCY0169197.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            13914..14852
                     /locus_tag="O0286_000811"
     CDS             13914..14852
                     /locus_tag="O0286_000811"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCY0169198.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(15023..17425)
                     /locus_tag="O0286_000812"
     CDS             complement(15023..17425)
                     /locus_tag="O0286_000812"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCY0169199.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(17425..18483)
                     /gene="pheS"
                     /locus_tag="O0286_000813"
     CDS             complement(17425..18483)
                     /gene="pheS"
                     /locus_tag="O0286_000813"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCY0169200.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(18864..19604)
                     /locus_tag="O0286_000814"
     CDS             complement(18864..19604)
                     /locus_tag="O0286_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCY0169201.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(19844..19951)
                     /locus_tag="O0286_000815"
     CDS             complement(19844..19951)
                     /locus_tag="O0286_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169202.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(19988..20311)
                     /gene="isdG"
                     /locus_tag="O0286_000816"
     CDS             complement(19988..20311)
                     /gene="isdG"
                     /locus_tag="O0286_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCY0169203.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(20330..21064)
                     /gene="srtB"
                     /locus_tag="O0286_000817"
     CDS             complement(20330..21064)
                     /gene="srtB"
                     /locus_tag="O0286_000817"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCY0169204.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(21126..22091)
                     /gene="isdF"
                     /locus_tag="O0286_000818"
     CDS             complement(21126..22091)
                     /gene="isdF"
                     /locus_tag="O0286_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCY0169205.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(22104..22982)
                     /gene="isdE"
                     /locus_tag="O0286_000819"
     CDS             complement(22104..22982)
                     /gene="isdE"
                     /locus_tag="O0286_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCY0169206.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DRAMTQLYDLFYKDKK"
     gene            complement(22979..24055)
                     /gene="isdD"
                     /locus_tag="O0286_000820"
     CDS             complement(22979..24055)
                     /gene="isdD"
                     /locus_tag="O0286_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCY0169207.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(24055..24738)
                     /gene="isdC"
                     /locus_tag="O0286_000821"
     CDS             complement(24055..24738)
                     /gene="isdC"
                     /locus_tag="O0286_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCY0169208.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            24947..25999
                     /gene="isdA"
                     /locus_tag="O0286_000822"
     CDS             24947..25999
                     /gene="isdA"
                     /locus_tag="O0286_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCY0169209.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT         8185 a         4925 c         3567 g         9415 t
ORIGIN      
        1 aatttatgac tttagaaatc cttttaaata tgtatacgat tcatgatttt aactaaaatt
       61 tcaaatttcc tgacttcttg gaatcttaat tttatacttt ttaataatac acttttaacc
      121 aaataatcaa catgattcaa ccatataggg ttatcaacaa tcacattctc caaaattaca
      181 attcttttca taacataaat gtgttattca ttccaattat cttataaaat tgtcagaaat
      241 acttttaaac aaacattcat gacacgacct cctagttaaa tcagtctata aactaaaaaa
      301 agccatagct acttctattt aaaaagtagt atgactgatt gtttcgtgat gtcgattttt
      361 gaaattgatg gtgccatgta aagtttgatc atcaataata acttcagcat atgtttcttc
      421 cattgaactt ctagattgag atatacttcc aggattaata acatgaacac cattaatata
      481 ctcatatttt gccacatgtg tatgtccata aaatgcaaac aaacaaccta attcaagtcc
      541 tttttcagct aataaatctc ttgttcgatt gacttgatat aaatgtccat gagtataaaa
      601 tgctttcacg tcattatatt ttgcgaccgc ttcattttca aattctgggt aaaaatcaca
      661 attgcctttt actctattaa ataagctaag ttccgtatca tcatacgcga attctgaatc
      721 tcctaaatgt aaatatacat ctgcatctgg gtgcatttca taaatttgat ataaaacgcc
      781 tgattcagta tggttatcac tcacaataat ccatttagac atttttatca ccttcaagaa
      841 aagcttgaag taaattaatc gcatttcgtc tatgactaat ttgccctttt tgttcttttg
      901 aaagttgagc catggtttta tctaatttcg gtacataaaa tatcggatca tatccgaaac
      961 cattttcgcc atattttcca tctgcaattt cacctgaaac agtaccttta aatacttttg
     1021 tttccatatc agggccactc atacttatga cacaaacaaa ttgcgcacga cgatcagttg
     1081 tattaccaag cttatttaat aatttttcaa tatttgcttc atcgctttta ttttcaccag
     1141 cataacgtgc agagtatata cctggctcac catttaatgc aaaaacttct agtccactgt
     1201 catcagctat gaccgtttta ttcaatgctt ttgcagcagc ttctgatttt aatatagcat
     1261 tttcttcaaa tgttgatcct gtttcttcca catcaaaatc tggtattagt tctgaaatac
     1321 ctattacgtg gtaatctgga aatattactt taaagtcatt tattttccct tgattattcg
     1381 atgcaataac aatctctttc attaaaaatc ctcctagtca ttcactgata tacgttccac
     1441 attgacagat aaatttaacc actctttgat aatgttagta atgtgagtag gatcacctgt
     1501 tgcaaaaaat cgatgatctg gatgttcagt ataacttgca tgttcattac taaatgttag
     1561 caatgcacta acttcacgag ccgtttctaa tccagacgaa atcactgtct ttttaccacc
     1621 aaaataatca tagataggtt tatagagcaa tggatagtgg gtacatccta aaatgacagt
     1681 atcagactca ctattacgcc aacgtttcag tgtttgatga atgacaatgc ttgtaattgt
     1741 tggatcacta tatctcattt gttctacaag tggcacaaaa cctggacagg caacgccatg
     1801 tacctctaca tgtggattga tacgtttaat atgcgtacga tatgcttcag atttaattgt
     1861 gccttccgtt cctagtacta atacattttg atttctagta gtcattattg ctgttctagc
     1921 acctggttca attacgccaa tcactgggat tgataaggtc ttttgtaaat attctaaagc
     1981 tacagcagtt gctgtattac aagcaatcac gagcattttt atatcaaatt ccattaattt
     2041 acgagcgatt tcaactgtat attgttttac ttgttctcct ggtcttggcc catatggaca
     2101 tcgtccaata tcacctaagt aataaatcgt ctcatttggc aactgacgca taatttcttt
     2161 agctactgtc aaacctccga caccagagtc tattacacct attggtttat tcatattcgg
     2221 tcatccttac ttttaattta atttttagtt tatcataact aagcattgga ttttagtatt
     2281 atgcactgtg tttaccattt ttgtcattat aatatttatt ttaaatcagc ccactatcat
     2341 attgtcatgt aatctgctta ttaaaaaaat cccttccaag ttattgtgta tctccattca
     2401 atttaatttt gaaaggaaca taacatttta actcaaaagg gattaatttt tattctactt
     2461 catggtctga accaaagaat gatttaaaca tgtggaatgt tgtttctctg ttcattgctg
     2521 caatggatgt tgttaatgga atacctttag ggcaagcatt aacacagttt tgtgaattac
     2581 cacactgctg taagccacca gtacccatta atgcatttaa acgttcatct ttagtcatag
     2641 atcctgttgg gtgcaaatta aacaaacgaa cttgcgagat tgcttgtgca ccaacgaatt
     2701 tattattttc agtaacatta ggacaaacct ctaaacatac accacatgtc atacatttag
     2761 ataattcata agctgtttga cgttttttct ctggcatacg tggtcccgga cctaaatcat
     2821 acgttccatc aattgggatc catgctttca tacgttttaa gttatcgaac attctagaac
     2881 gatcaacttg taagtcacgg ataactggga aagtattcat tggctctaaa cgaataggtt
     2941 gttctaattg atcaacaatc gcagaacaag attgtcttgc acgaccattg ataaccatag
     3001 aacatgctcc acatacttct tctaagcagt tcatatccca gacaacaggt gttgtttttt
     3061 cacctttaat attaactggg ttacgtctaa tttccattaa acaagcaatg acgtttaaat
     3121 tttcacgata tggaatttca aatgtttctt cataaggctt agaatcactt gtatcttgtc
     3181 gtttaataat taattttact gttttttgtt tcggtttaga ttgtgtttca tgttgtggag
     3241 tgtttttcac tgattgttca gtcattattt tttaccccct ttagacttac ttgtgtaatc
     3301 acgtttacga ggtggtatta aactcacatc gacgtcatca taagtaaact gcggtttttc
     3361 aaatgcgcct tggaatgagg ccattgtcgt ttttaaccac tcttcatcat tacgctctgg
     3421 gaattctggt ttataatggg caccgcgtga ttcgttacgg ttatatgcac caatcgtaat
     3481 aacacgtgca agtactaaca tgttccatag ttgacgggta aagaataccg cttggttact
     3541 ccaagtttga gtatcttcca tatcaatatc ttcataacgt ttcatcaatt caacaatctt
     3601 tttatctgtt tctaacagtt tttcattttc acgaacaaca gttacatttg ctgtcataat
     3661 ttcaccaagt tcacggtgta atttatatgc attttctgta ccgcgcatag ctaataattt
     3721 atcaaaacgt tcttgctctt cagctttacg cttttcaaaa atactttcgt ccatatcagt
     3781 atatgatcga tcaatatttg aaatataatc aatcgcgttt ggacctgcta ctgtaccacc
     3841 ataaatcgct gataacaatg aattggcacc taagcggtta ccaccatgtt gagagaagtc
     3901 acattctcca gctgcaaata accctttaat atttgtcatt tgatcataat ctacatatag
     3961 accacccatt gaatagtgaa cagctgggaa aatcttcatt ggtactttgc gtgggtcatc
     4021 accagtgaat ttttcataaa tctcaatgat accacctagt tttacatcta actcatgtgg
     4081 atctttatgt gacaaatcaa gatataccat gttttcgcca tttataccta atttttggtt
     4141 aatacataca tcgaaaattt cacgcgttgc gatatcacga ggtactaagt taccataatc
     4201 aggatatttc tcttctaaga agtaccaagg cttaccatct ttatatgtcc aaattcgtcc
     4261 accttcacca cgtgctgatt cactcattag tcgcagttta tcatcaccag ggattgcagt
     4321 aggatgaatt tgaatgaact caccattagc ataaatagcg ccttgttggt aaacaatgga
     4381 agccgctgat cctgtattaa tcattgagtt tgttgtttta ccgaaaataa taccagggcc
     4441 acccgttgcc ataataactg catctgaacc aaatgtttca atctcagcag ttgtcatatt
     4501 ttgtgcaacg atacctcttg cactatcatc gtcaccttta actatgccaa ggaattccca
     4561 tccttcatac ttcgtaacta atccatctac ttcatatgca cgaacttgtt catccaatgc
     4621 atataataat tgttgtccag ttgttgcccc tgcatatgct gttctgtgat gtaatgtacc
     4681 accgaaacgt ctaaaatcta atagaccttc atttgttcta ttgaacatta cgcccatacg
     4741 gtctaataaa tgaataattt taggtgctgc ctctgtcatc gctttaacag gtggttggtt
     4801 tgcaaggaaa tcgccaccat acactgtatc atcaaagtga atccaaggag aatcgccttc
     4861 ccctttagta ttgaccgcac cattaatgcc accttgggca caaacagagt gcgaacgctt
     4921 tactggtaca actgagaaca aatctacatg tgcacctttt tctgccgctt taattgttga
     4981 cattaagccc gctaggccac ctccgacaac aataagatgt ttctctgcca taaaaatttc
     5041 actcccctaa attttcaatc tatatttgtt aaatgcgatg tattacataa aggcaataat
     5101 tgcagtaaca ccaatatacg aaataactaa aaatacgatt aatgaaaccc atgtaaatac
     5161 tcgttgtgat tttggagatt gaagtccacc ccaagtaact aagaatgacc ataagccatt
     5221 tgcaaagtgg aacacaacag caataataca aataatataa aatattgccc atccaggatg
     5281 ttgcaatgtt tcgtgcatta aatcgtaatt cacttctttg ccgtaaaatg ctttttgtaa
     5341 acgtgtttgc cataaatgga taccaataaa gataaatgtt aagataccac tcactctttg
     5401 gaagaagaac atccagtttc taaaaatcga gtaatgtcca acattttctt ttgctgtaaa
     5461 tgcaatgtgt ataccaaaca aaccgtgata taacaacgga atgtatataa ataaaaattc
     5521 tacaataatt agaaatggta atgattccat aaagttagat gccttattaa acgcttcagc
     5581 accttgtgtt gcttggtgat tcactaataa atgaacgacc aaaaatgcac ctattgggat
     5641 aatacctaat aacgagtgaa tacgtcttag ataaaattca ttttttgatt gagccaaaag
     5701 gagtcccccc tgtgaacgaa tatttaattt attgagctat ttatattaaa cgtacgctta
     5761 accccctaaa gtgatatcgt ttctagcgtt gtcattatca ttaagcgaaa cattttaaag
     5821 acaaatacac actgcacgat caccaaactg catgtcgaac aatgtaacat ttggattcga
     5881 tatttaaaat tgcttgtgat gataaacttt ctcatttaga aaacgcttcc acgtacattc
     5941 aaaaaaataa ctttgttaac catattgtaa cattatttca tatattttgg ggcatgagaa
     6001 tgattctcac gcccagtaat ttatttatgc aattgttcat gtaggttctt tgcgacgttt
     6061 tcaggaatac ctatattttt aaaatcttca agtgtagctt ccttcatttt cttgattgaa
     6121 ccgaatgaac gcaataataa tgttttacgt ttgttaccga taccatctat atcatcaagt
     6181 attgatttca agcctgtctt ttgacgtgtt tgtctatgaa atgtgattgc gaatctgtga
     6241 acctcatctt ggatacggtg caacaaataa aatgcctggc tatttttctt cagtggtaca
     6301 atttctgcac tagcgccata taataattca gatgtttggt gtttatcatt tttctgcaaa
     6361 cctgcaacag ggatatcaag acctaattcg ttttgtagca catcaataac cccgttcata
     6421 tgtcctttac caccatctac tattattaaa tcaggtaatg gtaatccttc gtttaaaacg
     6481 cgagaatatc gtcgtcttac tacttctctc attgatttgt aatcatctgg acctttaacc
     6541 gttttgattt tatactttct ataatttttc ttatctggtt taccgtcgac aaatgtaacc
     6601 attgctgaca ctggatccac accttgaata ttagaattat cgaatgcttc aattctaatt
     6661 ggtgtttgaa ttcccatttg tgttccaagt tcttcaatag ctttaatcgt tctggactca
     6721 tcacgtgata ttaattcaaa tttattattt aaggatactt tagcgttatg tgcagctagg
     6781 tcaaccatat cttttttggg acctcgcgcg ggttgaacga ttttagtgtc cacaacagat
     6841 tgaatcattt ctttatccaa attacgtggt acatgaactt ccttaggtaa aatatgttgg
     6901 tttaagctat aaaattgtcc aataaatgta taaaattctt cttcttctgt ttgctgtaat
     6961 ggaatcatcg ttgtatctcg ctttatcata ttaccttgtc gtataaagaa aacttggata
     7021 cacatccatc ctttatcaac actataacca aagacatcac gaatcgtttt atctgatgac
     7081 ataatttttt gtttgtttgt cagattttga atatgttgaa ttaaatctct atattcttta
     7141 gcccgttcaa aatcaagtga ttcacttgca gttaacattc gctcttctaa actttttaaa
     7201 attgttttgt cttccccatt cagaaaatca gtaatttcct tcgtcatttg tgcgtattta
     7261 ctcaaatcaa cgtcatatac acatggtcct aaacattgtc caatatggta ataaagacat
     7321 aatttatctg gcatcttatc acatttgcga tatggatata ttctgtctaa taacttttta
     7381 gtttcttgag cagaatatgc attcggatac ggtccgaaat atttgccagt accttgtttt
     7441 acagttctcg tcactagtag tctaggatat ttctccttcg taattttaat aaatggataa
     7501 cttttatcat cctttaataa tatattatat cttggttgat attgtttaat cagattcaat
     7561 tccagtaaaa gtgattctgt ttcacttgac gtgacaataa actcaaagcg acgtatttca
     7621 ccaaccagtc tcgttgtttt agcatcatga gcacccgtaa aatatgatcg caatcgattt
     7681 cttagctttt tagctttgcc aacatatatc acttgatcat tacgatcttt cattaaatag
     7741 catcctggtt ccataggtac gacatttaat ttatttttaa ttcgttgctt atagtcttcc
     7801 aaaaatatcc ccccttctaa ttttaaaatg aatgatgcta caaaagtgct tcaaatttat
     7861 caccactaat tctagttaat aatcaacatc ttcgagtatg acttttcatg cacatcatgt
     7921 aatattgaac atggttttat caaaccaact gctacaaatc tatcagcatg ctagattatc
     7981 atttaacgtt catagattta ttaatcccac cattctcaag tcgtaaacaa tatgatgact
     8041 tatcattcaa ctttttatat taaaaacaca aacaaaaact ggagcaattc attaaagaaa
     8101 tgccccagtc gtcattggtt gtaacttata aatgtttatc taaaacttca gctaagtttt
     8161 cttttggttg gaaaccaaca actttatcaa ctggttgacc gtctttaaag acgattaatg
     8221 ttggaatact catcacttca tatttagctg cagttgatgg attttcatca acatctaatt
     8281 ttaaaatgtc agctttacct tcatagtcag ctgctaattc ttctaatacc ggagcgatca
     8341 ttttacatgg accacaccat gttgcccaaa aatctactag ttgtacacca gattctactt
     8401 ttgaatcaaa atctgcatct gttactttta cgattgccat aaatgccaat cctccttaat
     8461 tattattatg atgttcaaat tatatcagat aacatgcttc atgtatttct atttgctcaa
     8521 tttttatgct tatttcaaat aacatgtttg aaatgtattc ttgttgcttt acattatttt
     8581 aactgcagct atttaattta tcaaattata atttatttta gtgttgcaac ggtaacgcca
     8641 aatccacctt cgcttggcat accacctcta aagtcactaa cacttttatg ctttttcaaa
     8701 tgttgttgta cacctttttg aagtgcacct gtacctttac catgaatgat ataaacttgt
     8761 tcgtaattac ttaaaacggc ttgatctaaa tattgatcta gttcaattaa agcatcctca
     8821 taacgatagc ctcgtaagtc aagttcagtt ttaattgttt gacgattttg acgtgtaacc
     8881 attttcgttg gcttaacttt ttctttttgt tttttctcta aatcttcaat aggtaacttc
     8941 attttaataa ttcccatttg aacaattgct tcttcatcat tgacaatttc taaaacttca
     9001 cccttttgac cgtaagataa tacttttact tcatcaccag caacaatttt atcgtatttt
     9061 tgcttttgta cattttgctt tatagatttc gcttcataat gatcatctaa tcgtttcttc
     9121 ttatcaatca attcatgttc tttaacatct gcaccttttt gttcacgcaa ttgtcttaag
     9181 tctttaataa tatcgtcagc ttcttttgtt gcagccttaa tcttctgatt tgctttttct
     9241 ttcgcttcct ctattagaga cttttcataa ttttggaatt gttgatactg cttagataaa
     9301 tcatcatgca cttgctccgc ttcttttaca agacggtcca gttctaacct ctgtgtctct
     9361 acacgtttgt aattacgctc taatgattca atcatttcat ttatttcttt ttcatcagta
     9421 ccaatcatcg tcttagcctt attaataata ttcaaactaa gacctaactt tttagaaatg
     9481 tcaaaagcat ttgaacgacc cggcacaccc attaataact tatacgttgg actcaaagta
     9541 tctacatcaa attctacact cgcattcata acgccttctc gattataact atatgcttta
     9601 agttcaggat agtgcgtcgt tgccattact agagaaccaa tttttctaac atgatctaaa
     9661 atgctcattg ctaatgcagc accttcactc ggatctgtac ctgcacctaa ttcatcaaat
     9721 aaaactaaac tatgtttgtc tgcatgcttt aaaatttcaa ctatattcgt catatgagat
     9781 gaaaaagttg ataatgattg ttctattgat tgttcatctc cgatatcgca atatacattt
     9841 ttaaatacac ttaactgact accatcaagt gtgggaatca acaatcctga ttgagccata
     9901 acaataatta aacctaatgt ttttaatgtt acagttttac cacctgtatt cggtcctgta
     9961 ataattaccg tttcaatatc ttccataaat tcgatggtat tagctacaac agtctcacga
    10021 tttaataatg gatggtatgc tttaggtaaa tatacagtac ggtcctcttt aaatatcggc
    10081 tttgttcctt taatacttct actatatctc gcttttgcga ttaaaaaatc taactgaccc
    10141 atgacttgtt ctgccacaag tagtgcatct ttgtccgcag ccacataacc agttagttgc
    10201 gttaaaatgc gttctttttc aattgcttcg tcatgacgta atcgactaat ttgattattc
    10261 atttcaacaa ctgatgatgg ctcaatatac aatgtttgtc ctgaagcaga ttgatcatgt
    10321 acaatcccat taaaatcttg tcgatattca gctttgacag gtataacgtt tctttcattc
    10381 ctaactgtta caatagcatc tgataatttt ttctgatttg cttggctttt aacaatacgg
    10441 tccaaatttt gtctaatacg ttgattcgtg ctagaaattt tacttctaat cccttgcaat
    10501 tcataactcg cattatcata taaatcatac gtatcgcatg tttcatttat ttgttgaaaa
    10561 agatcagtta acacaggtaa ttgattcatc ttgtcatcta atattgggta tttaacacct
    10621 tcatcttctt caaccaattg attataaaat gtcttgaatt gattttgtac ttgaattaat
    10681 ctttttatca agttaagctc tgatacattt aaaacgccgc caatatcagc gcgatgaatg
    10741 aatgctgata ctttagataa gccactcaag cttggtaaac gatgcttatt atagatttga
    10801 gcaatctcat ccgtttcttc catttgaaaa acaaccgttt caaaattagt agctggcatc
    10861 atttgattga ccttttccaa gcctaagtca ctaatagttt cattggcaac gagtgatttt
    10921 attttttcaa attctaagac gtctaatgtt ttttgtctca taaaatccct ctatttctta
    10981 agttttgtat tattttcaat aaagtcttta aaagcttcac gcgataatgc gttaatcact
    11041 ctatcttttg ttacaaatcc tttttgcgca gttgctacgc cataattcat aaaatctaaa
    11101 tgatttgtat gatgcgcatc agtgttaata gttaatttca catttggata tttacgaacg
    11161 atatcagcgc tcagatccag tcgatgtgga ttggcattaa tttctaatac tgtattcgtt
    11221 tcttcagcta atgccattaa ttgttcaata ttcggtttat aaccatctct tctacctata
    11281 atacgccctg ttggatgcgc tatatgtcgc acgtatggat tgcgacatgc attagctaat
    11341 cgttccataa tttgttcttc tgattggtta aagctttgat gaatagctcc aattacataa
    11401 tcaagttgtg ctaaaatttc atcatcataa tccagcgagc catcaggtaa tatatccatt
    11461 tctgtacctg aataaatatc aatttcacta tattctttat ctaaagcctt aatttcttcg
    11521 ttttgtctta aaagtctttc tacttgtaag ccattagcaa cacgtaaact ttgtgaatga
    11581 tcagtaatta ccatgaattt ataacctttt gcgatatttg cttctaccat gtctcgaata
    11641 gaaaacgcac catcactata cgttgtatgc atatgaatat caccattaat atcatctatt
    11701 gtaatgatat tacttagatc tttatcaaat tcgctaccat cttctcgcat agcaggtggt
    11761 ataaaattca cattaaaatg ttcatatatc ttggcttcac tatcatattg aattaatgta
    11821 ccatcagctt gttcaattcc atattcactt actttttcat cacgtgcttt agcaagttgt
    11881 cgaattctta tattatgttc ttttgaccca gtaaaatgct gcaatgtatg ataaaaagca
    11941 cttggttcaa ttaatcgaaa atcgacacca atcgtttcat catcatacgc taattctaat
    12001 gaaacttttg tgttccccac tgcaacttct tttactttat tgggaatatt taataattgc
    12061 tgctgcactg cttttgggtt atcggtactt attatgaaat ctaaatcttt gctcatttct
    12121 ttaaaacgac ggaagcttcc tgcagatgaa tattgatcga tataatttaa tgtatctata
    12181 taatcaatga tttcttgatt aagtcttctc atttgatcaa ttggatatct atctttctta
    12241 gcaccaagtt gtttcacagc ttctaatatg ttttgttccg ttttcttagc aaatccgctt
    12301 aattcactaa cttttccatt ttcacaagca acttgaagtg acgctttatc aacaatattc
    12361 aactctttat atagcttagc aattttcttg cttccaagtc cttgaatttt caaaagtgga
    12421 ataagacctt ccggaacttc ttcctgtaat tgctgtaaat actgagattc accggtctca
    12481 cggtaatcat tgattacttc tgcaacacct ttaccaatgc cttttaactc cgttacatca
    12541 gatatttcat ctaatggtcg ttcatctaat tcaagacttt gagctgcttt tcgatacgct
    12601 gatattttaa aagtattttc cccttttaat tccatataag tagcaatttg ttctaatagt
    12661 ttgataacat ctttttttgt cataataaca ctccataaaa agaagaccag gacgtatcat
    12721 taatatatac ctttgtcctg acctcttatg ataatttata aattaatggt gaaagccgat
    12781 aaatatggga tgtgaaaaat caatgaatga ctcacaatag atattttaag ctgatgttgt
    12841 aatgcttcgt taggatataa tgccaataga tataacgtaa agtgtaagac aattatcgtc
    12901 ataaacacac caactatcat tcccattgca cgactgaaaa tatgaatgtt ttgatacgct
    12961 attattttat caaaagttac gataattaga tataaaatga acttacaaaa caatgtaatc
    13021 attaaaaaag ctacaatcgc ttcaaatcga ttttgtagat gattaaaatg aaacgcaaaa
    13081 gttgtattaa atgctgttgt tttaggatat ggaataaata caattaatct ttctacaata
    13141 gatttgtaaa attgactggc aatccacaat gatacaatcg ttgcactcaa atgtatcata
    13201 gataaccaaa aacctcgtct gaatccaacg atgacaaaat acacaaagaa aatgattatg
    13261 ataaaatcaa tgaccattta ttgctcacgc tgctgcaatt tgtgaatttg ttgtttcaaa
    13321 cgtcgatttt cttcttctag tagtactttt tcatgcataa tattcacagc agttagtatt
    13381 gcttttcttg aagtatctaa acctgctgct ttatacccta attcttttat tttatcatca
    13441 actaaatgtg ctacatatcg tatgtgctct gggttatctt ccccaacaat tgtaaaaagc
    13501 tgatcattaa ttgatacatt taccttgttt ttaaactgtg tcatttataa tttctcctga
    13561 tccttttttt aaaatctaaa ttcacgttat aaaatatgac tggatagttt gtctgaattt
    13621 gatactaata ttgttatatt gcaattatga taaaacaaca acacaatctc tatagatgac
    13681 ttaatgttct ttttataatg aaataatgta aagaattttc tattcaatac tttatcatgt
    13741 ttaaattgtg tcactataac attttcataa acattataca tgaccactat gtattttgta
    13801 agtatccgca attaattctt tacaacatac ataaatgttt ctgacgttat tatcatttat
    13861 gatatgatta tttttgctaa agacaatgaa attttatgaa aggatttaca caaatggcga
    13921 atatcgtttt taaattgtcg gataaagaca taacgacatt aatgtcacgc atttcttttg
    13981 atactgagaa tttacctcaa ggtatgaaag cacgtgcaaa gtatcaaaat acaactgtaa
    14041 atatttacca atcaggtaaa gtcatgtttc aaggtaatca tgcagaagct gtgtctgaag
    14101 aacttctacc gcagcattct caattgaata ctaataaaac taaaaagaaa aatatggcta
    14161 attcttttct tgaacaaact ttaatgtatg atcaatttaa ctgtattggt agtgatgagg
    14221 ctggaagtgg cgattacttt ggtccgttga ctgtgtgtgc tgctttcgtt accaaagagc
    14281 atgtccctat tttaaaaact ttgggtgtag atgattcaaa aaaattaacc gataccaaaa
    14341 ttgtagaact tgctgagcaa ttggttactt tcataccaca ttcattatta acactacaca
    14401 atgaaaaata taatattcag caagcaaaag gctggactca agtaaaaatg aaagctgccc
    14461 ttcataatga agccatcaaa aatgtacttg aaaaaattga ttcgtctcaa ttagactata
    14521 tcgtcattga tcagttcgca aaacgcgaag tgtatagcca ttacgcatta tcagatatac
    14581 cacttccaaa gaaaacaaaa tttgaaacaa aaggagaatc gaagtcatta gccattgcag
    14641 tggcaagcat aatttctcga tacgcatttg ttacatatat ggatcaaata tcaaaaaata
    14701 ttaatatgac gattcctaaa ggtgccggtg ctaaagtcga tgtaatcgcc gccaaaatta
    14761 taaaaaaata cggtctttca cgtttagata ccatttcaaa aaagcatttt aaaaatcgtg
    14821 aaaaagccca aaaaatttta aagcctcttt gatttttaat cattagaggc ttttatgcag
    14881 ttgtgcgaag catcatctgt attccgctgt tcctctttga ctttttaaat gcattggaac
    14941 caaattggtg aacccaaaaa taaaacccca tgttagggat atcaatctac ttcagatatc
    15001 cttatacatg gggtttaaat cattatctaa taacagcacc ttgttcaatt aatgctgctt
    15061 caatttccgc ttgtactttt gaaacgcgct catctgtcaa tgtttcttct gtgtctaaat
    15121 aatttaaacg tattgcaatt gattttttac ctttttctaa atgttcgccc tgatatacat
    15181 caaagacaag tgtatctttt aatatattgc caccatgtgc atgaatcgtt gataataaat
    15241 cagctgctgg aatattttga tctacttcta atgcaatgtc acgagacatg cctgggaatc
    15301 tcggaattgg ctggtaatta atgtaaccta ccgacacagc cattaatgca tcaaaattca
    15361 actcaaaaac atacgtacgt tttaaatcat tatcagctgc taatattgga tgtaattcac
    15421 caataaaacc aacaacttta ttctctaata agatttcagc agtacgacct ggatgtaatc
    15481 catcaatatc agcacggcga taactaaatt caagatttaa cttttcagat actcgatcca
    15541 cgacaccttt tgctaaatag aaatcaaccg tttctttctt atcttgccat tgattgacta
    15601 cataatctcc agttaaaata ccacttaaat attcaacttg atctggtagt tcaccttctc
    15661 cattagcaaa gaagacattg ccgatttcaa ataattttac atctttattt ttgcgtgcca
    15721 cattatatga tgccgcttcg attaaatgtg gtaataaact ttgacgtaat gacgcatgcg
    15781 cttcactcat tggcatcaat aaatcaattg tttgacgctg ttgcatcgaa aatgcagtag
    15841 catcttcttt agaaactaac gaatacgtaa tagcttggtc taatccagca ccttctaaca
    15901 cttctttaac cattctagtt ttatattggc gatcagttag ctgaccacta gtaactttat
    15961 cgaagacagg taacgttgat ggaatatcgt cgtatccata tatacgtgca acttcttcaa
    16021 ttaaatcttc tttaattgta atatctttac gacgtgatgg tactagcact gtaataacat
    16081 catcatttat ttctgtatca aaccctagtt gattaaaaat agtaacaata tcattttgtg
    16141 acaaatcaaa tccaatagtg cgattaattt tatcagcagt gatgtcgatt ggtgtaataa
    16201 atgcaccaag ttctcctgaa gacactctat cttttagcac ttttccgttt gcataagttt
    16261 gtaataaata acatgcacgg tcgactgctt catctacaaa ttcagtagct attccttttt
    16321 caaaacgact agatgattca ctgcgtaaat ttaaacgtct tgatgtatga cgaattgaaa
    16381 ctggatcaaa aatagcacct tcaatcacta tatttgatgt ttgttcttta acttctgaaa
    16441 aatcgccacc cataacacca gctaatgcaa ttggagtttg tccattagta atgacaatat
    16501 cgctcgttaa taattcacgt tctgtatcat ctaatgttgt cattttttcg ccttcattag
    16561 cttgacgaac aacaatttgt tgtgaaccaa tcgcatcttg atcaaacatg tgcaatggtt
    16621 gaccgtattc taataacaca taatttgaaa tgtcaacaac attattaata ggacgtatac
    16681 ccgcttttat taagcgtgct tgcatccaaa ttggcgaggg ttcaattgtc acgtcgtgaa
    16741 caacacgtgc actataatat ggtactttat cttcattttc aattgtcaca gtcagttcat
    16801 catttgcaga taaatcaagc tcatttgatg ttgtctctgg cttagtcatt tttgtattat
    16861 ataatgctgc aacttcataa gcagtaccta tcatacttaa agcatctgca cgattcggcg
    16921 ttaaatcaaa ttccattact tgatcatcta aatataaagc ttgtaaggca tctgttcctg
    16981 gaacttgggc ttcactaaat acataaatgc ctgattcaaa acttttcggt atatagttac
    17041 ttgaaatacc aatttcttgt aacgaacaaa tcataccttc tgaacgttca ccgcgtaatt
    17101 tggcacgctt aattttaata ccaccaggca atctgccacc tactttagca acaatgacat
    17161 attgtcctgc atcaacgttc ggtgcaccac aaacgatttg tacaggttcg tcttctccga
    17221 tatcaacttg gcaaacattt aatttatcag catcaggatg tttctctttt gacttaacga
    17281 agccgacaac taaatttttg atatcttttg tgtagtcaat taaatcatcc acttcaatac
    17341 ctgtgcgcgt aatacgttct gccaaattac ttacagaatc atcgattgtt acatattctt
    17401 tcaaccattc atttgatatc aacattacat gtcacctcta tcttctaccg ctttaaattg
    17461 atctaaaaat ctcacatcat tagtatagaa atgacgaata tcttctatac catatttcaa
    17521 cattgcaata cggtctggtc ccataccaaa tgcaaatcca gagtactctg aagaatcaaa
    17581 accagccatt tctaatacat taggatgtac cattccagca cctaaaattt caatccatcc
    17641 tgtgtgttta cacacattac aaccttttcc tttacattta aaacatgaca catcaacttc
    17701 tacagaaggt tcagtgaatg ggaagtaact tggacgtaaa cgaatttcac gatcagcacc
    17761 aaataatttc ttagctaaca attctaaagt acctttcaaa tcactcattt taacgttttt
    17821 atcaacaact aatccttcga tttgtgtaaa ttgatgacta tgtgtcgcat catcagagtc
    17881 acgacgatac actttaccag ggcaaataat tttaactgga ccttgaccat gacgtgattc
    17941 catcgtacgt gcctgcactg gtgatgtatg cgtacgtaat aaaatttcat ccgtaatata
    18001 gaaactatct tgcatatcac gtgcagggtg tgatttaggt aaattcagca tttcgaagtt
    18061 ataatgatct tgttcaactt catatccatt cacaatttca taacctaaac ctaagaataa
    18121 gtcttcaatt tcttctattg tacgtgttaa tggatgcttt gaaccgattt caatatgacg
    18181 acctggtaat gatacatcaa ttgtttcttc agccaattgc ttatttaatt tttctttaac
    18241 taacatctgt tgtctttcat ctaattcatt ttgaattgtt tgacgcaatt cattcacttt
    18301 ttgaccaaac gcaggtttat cttcattcgg caaatccttc atcaatttca ttagtccgct
    18361 aactgaccct tttttaccta agtatttcac tttaacttct tgcagtgcac gttcatcatt
    18421 tgcttcatta atatctacaa gcgcttgttg ttttaactct gacattgttt gttgttcaga
    18481 catatgtcac tgacctccct attcgaacta ttcattttct atttatcata tttgttgttt
    18541 aactaaaaaa agactttcat cctcttaaaa aataagggac gaaagtcatt tccgtggtac
    18601 cacccaaatt tatgcctaag catacacttg tttaatattg ataacggatt gtctttccga
    18661 cttaaatctc tttaagtaac caaaaaggtg aaaagacttt aaataattgg aataacttgc
    18721 agtcacgatt attctcccta aaacaattat ttatataaat gtccatgcct ttttattggt
    18781 ttatattcaa ttgtgtatgc attcacgtag aaatgttctg ttaaacaatt aattataatt
    18841 atatcggaaa actttcaaca cggtcaacct ttcaaatgat aaagtaaaat actacctgca
    18901 atcgctacat ttaaactttc agctttacca taaataggta tgattaaatt ttgtgtagta
    18961 tgtgctaata attcaggatt aacaccttct ccctcattac ctaatagtaa tgcaaaagaa
    19021 tcacttgaag taacttcttt gtatgccact gcgttttcaa gtgctgtacc ataaacagga
    19081 ccattaaatt gagtaataaa cgtatcgaga tcttgtgtca taactggcaa atggaaaaca
    19141 ctaccttgac tcgctcgcaa cactttatct tgataaggat ctgtcgtacc cttctccatt
    19201 attacagcat ccattccagc agcatccgca gttctaatta atgtgcctaa atttccagga
    19261 tcttgaacac gatcgatgag caatacttgt tgtgctttag aaatatcata atgcggcttc
    19321 tcaatgattg cgaaaaaccc ttgtggtgtc actgtacctg ataaagattc agcaactttc
    19381 atgtttattt caaaaacttc ttgcgcgtat gcgataattt gctgatctaa tcttgccggt
    19441 tcaattgcaa ataattgtgt aattacaatt ccactttgat aagcttcttc aattaaatgc
    19501 acaccttcaa ttaaagctaa tccagtttta tccctctcac gtttcttttt tagcttgttc
    19561 gcttgtttaa ttctattatt ttgtgcagaa gtaatttgtt ccattgatag ctcctcgctt
    19621 tatttttaaa aataaaaata ttaatcatta ataagatgaa aacatttgat tgtatagtta
    19681 atattaatta atcgctttta tcactcataa tatttcaaat tgtataaatt tcttttatcg
    19741 atactactac tataaatcat acgccccaaa atatcattat taattctttt cttcttcaaa
    19801 ataaatcaaa atgatataat tgatgattat tttcaaagca cattcaaatc aaactatgtt
    19861 ttagcaattt gttgttagca tgtttgtgtt cattaaaaaa acgaccatca tcggtatcat
    19921 gtatggtcgt tacaaaagct aacaatacca attgtcataa caagtactgc aacctcttta
    19981 aattcaatta tttcatgtaa ctatagccta tatcatatgt aattactttg ttatttataa
    20041 tcgggctact ttcatcttca tttttacttc taacatgttt atgcgctgct ttaaagacat
    20101 cagattttaa ccaatccgta aaagcttgct ttgatttcca aactgttaaa attttcactt
    20161 catcaaaatc ttcttgttct aaagtttgtg taacaaacat gccatcaaag ccttctaatg
    20221 tttcaatccc atgtctcgtg taaaatcgtt ctataatatc ttttgctgtt ccttttgtta
    20281 acgtcagcct attttctgcc ataaatttca taattatcct cttttctgtt taacttacct
    20341 taattatttt tgcgacaaca acaattcttt tcgtcgtttc actatatgca tcttcgcacg
    20401 ttgataaagt cattattcta tcttttaccg ttacattaac atctgaatta attacagatt
    20461 tacgttttgt ctcatctaaa aattgttgat aatcttgatc attttcaaaa tctgtacgta
    20521 tgtaattatc tttagtagta gttttatatg cactaaatac ttgcaattga tatttaccat
    20581 atttattgtc aaattcaatt atcttgtgtt tttcataaaa cgattgcttt aaataatctt
    20641 ctaacacatc aaacatcgta ttatcaccga catggtgccc gtataaaata gtattatgat
    20701 ttaaattctt caattcattt ctaaaatcca taaaaatact acctttacgt cgatgttctc
    20761 gctcaaaatc taaatttaaa taatcgtgat ttgtcttacc ttgtagtact ggataattta
    20821 atgatgttcc tgataatttt atccatccaa caatgtcttt atttattttt tcaagtgatt
    20881 caaattgtgg tctcacatgt tcttgatgtt tgctcatcag catttgaaat ttttgttgta
    20941 atttctcata atttgcgcgt tcttgcttgt cttcaatata tgtttgaaca attttgtaac
    21001 caaaaatgat aataattaca accaataaaa tttgtacaat agttaaaaat cgcttcattc
    21061 tcataaaaat cctcttttat taacgacgtt tcttcagtca tcactaaacc agttgttgta
    21121 ccgttttaga ttcgatttcg ttgactttga caaattaagt aaattagcat tggaccaccg
    21181 acaatcatta aaatagcatt ggctggaatt tctaaaggag gctgtatcac tcgtcctaat
    21241 aaatcagcca ctaacaatag ccatgcacca ataactgtag aaaacggaat aagtactctg
    21301 taattgcccc caactagctt tctaaccaca tgtggcacaa taatacctaa aaaggctagt
    21361 tgtccaacaa tcgcaacagt tgcacttgct aaaaatactg ctaataaacc tgttaaccat
    21421 ctgtaacgat caatattaaa accgatactt cgcgcttgta tgtcgtctaa atttagtaaa
    21481 ttcaatttag gggacaatag taatgttaat attaatccca ataatgctga tactgctaat
    21541 atgtatacgt cgctccatat tttcattgtt aagccttgag gaattttcat taaagggttt
    21601 tgagttaaaa tttctaaaac accatttaat aatacgaata acgcaacacc tactaatatc
    21661 atacttacag cattgaatct aaatttagaa tgcaacaata taattattaa aaatggtatt
    21721 aaacctccaa taaaacttaa taatggtaag taaaagtaca attgtggaat aaacaacata
    21781 caaagtgctc tcattataag tgcacctgag gaaacgccaa tgatattcgc ctctgccaaa
    21841 ggattttgta gtgctgcttg taataatgct ccagaaactg ctaacattgc gccaaccatc
    21901 aatgcaatta atatacgtgg caatcgcaaa tcaatgattg aatccactgc ttcattgcta
    21961 ccagttgtaa attttgtaaa taggtcatta aatgacaatt taattgtacc ggttacaaac
    22021 gaaatataag cagttgcgat taaaatgact aacaaacata aaaatagtag tttcttttta
    22081 ttttttatca tcatatcaac tcactatttt ttatccttat aaaataaatc atataattga
    22141 gtcattgccc tatcagcatc aacatttgct gtaataccga atggcacttc ctctaagtca
    22201 taaacatgat tatttttcac agctttaaaa tgtttccaaa tatcattttg tttaaattct
    22261 ttttgaaaca ttttcttaac ttcttcaggc attccgtgtg gtaatcgtaa aataatatct
    22321 ggattgatat tcaacaaatt ttcagtatta gacgaaatat attgacgatc tttcacttta
    22381 ataacatttt ctccacctgc tattttaact aaatcaccaa tatatgattt atcagttgct
    22441 actaaatagc tacccggtac acccattaat attaatactt tgggatgttt cttttgttta
    22501 gctgctttat tttcaatttt ttgctttaca gaatttaaat ggtcatttaa ttcttttgct
    22561 tgtgctttac gattaaattg atcacctaat tgtgtaatcg acttttgcat cccttttaaa
    22621 ctatcaaaat cataaaagta gcctttcata tttaattgtt tgtaaaatgg ttgcatttca
    22681 tctttaatcg ttgacacact caaaacatgt gttggtttta actttttaac agcttcaaca
    22741 ttcggctcca ttggttgacc aatttccggt acatctttat aacgattagg caatgtctta
    22801 tatgacgtgg gtttgccgac aattggtaaa tccaatttgt ccaatgtcat tgtcaatgca
    22861 acagttgttg gtacgattct gaattcgccg gatttagttg attcttgaga actggaagat
    22921 tgacagctgg ttaagataac gacgcttatc actaaaatgg ttaaatactt tatgattctc
    22981 aaacttggtt acctctcttt ctgctgtata aatgtgccga aataagtaaa atagttgcta
    23041 acacaatgcc agctgcaaat aacatatatt gctttttatg atttggttca tcaagttctt
    23101 taatatcact agataattga ctactattct tgctatcatc atctctatta aaagctggta
    23161 acgctttttc tttttcaact acttttttct tttccttttt tgatttttca tctttatgtt
    23221 tcggttgctc tttgtgatta ttttcaacct tttttattgt tggagggcta ttttcaactt
    23281 ttaatgtacc tgatgcttta ggtcgctcat tttcaatctt tttattactg tcttgttgca
    23341 ctttatcttt ttgtacagga atatcattta atcctttttc agtaaaaagg ctagtacgtc
    23401 tttccttagt atctgatttt tgactatctt ttttgtcatc aatatctttt ttaagctgat
    23461 cttgattatt ttcttgattt tgatgttttg aaggcacttc ttttgcatca tcatgttgta
    23521 cttttttctc tttaaaatga atagaataca ccatttcttt gttatctaga tttttctttt
    23581 cagccaacgt tttaacatgc atattgattt tatccatatg ctgttcaatt aaaattctta
    23641 ctattgtgtc atccttttca tcttttataa caaaaggaaa ttcttcattt ttgtcatttt
    23701 gaaaaacaag ttgttcttta tctatttgac cttttagctt tacttgcaag taaattttac
    23761 cagtcatatc tgaaatcaat tgcgcctttt cgtcaactag ttgttgttcc tcagttacat
    23821 ttttattttc ttgattaaaa attagtaact ctaacgcttt attctctttt atcttttcat
    23881 caaaaattga tgtagttaat tctgttttat cttttgcttc aggttgatta aatttggctg
    23941 atggatattt tatctcatca gccaacacgc taccaatcat tgttgtatat gtaagtatac
    24001 ctaagctaat aatagctata atagattttg tagcaatttg tttaacattt ctcattattc
    24061 cacattgcct ttagattttt ttctaacaaa caatgtgatt gcgattaata atgctaagga
    24121 tgcaactggt attgcatata tatatgcagg tgtaccagca tttgtttgtg gattttctac
    24181 tttgtcttta ttcgaactat tagattcact ttgaccagta gtcgttccat cagatccttt
    24241 gtcactacct gaagcagaat ttttatcatc tttacctggt gcattagcac ctgctacatc
    24301 agttggtcca ttaaatttat atgtaatgtt gtaatgatgg tcatatttga atggctttcc
    24361 atttactttt tcatcgatat aaacgtcaat ttttccatct attttaccgt tcaacttact
    24421 tacttcaaat tcagaagtgc gttcatcttt ggcagtgttt ttactaataa tattttcttt
    24481 atgtccttcg atactcattc cagtaatcca atgactgtgg ttgacagtta tttgaacata
    24541 caatttacca tttttcttaa tgtactttgc cggtttatta aaatagtcat tagcaattga
    24601 cgtgtcattg gtattgtatt tgtaaacctc ataattcaaa gtaccgctat ctgcggcatt
    24661 tgcagaatta ctgaatgtcg cgatgatgat aattaacgct aaaatcgttg tattaaaaac
    24721 ttttaaaata tttttcaaaa cataatcctc ctttttatga ttgcttttaa gtctttagta
    24781 aaatcataaa taataatgat tatcattgtc aatatttatt ttataatcaa tttattattg
    24841 ttatacgaaa atagatgtgc tagtataatt gataaccatt atcaattgca atggttaatc
    24901 atctcatata acaacacata atttgtatcc ttaggaggaa aacaacatga caaaacatta
    24961 tttaaacagt aagtatcaat cagaacaacg ttcatcagct atgaaaaaga ttacaatggg
    25021 tacagcatct atcattttag gttcccttgt atacataggc gcagacagcc aacaagtcaa
    25081 tgcggcaaca gaagctacga acgcaactaa taatcaaagc acacaagttt ctcaagcaac
    25141 atcacaacca attaatttcc aagtgcaaaa agatggctct tcagagaagt cacacatgga
    25201 tgactatatg caacaccctg gtaaagtaat taaacaaaat aataaatatt atttccaaac
    25261 cgtgttaaac aatgcatcat tctggaaaga atacaaattt tacaatgcaa acaatcaaga
    25321 attagcaaca actgttgtta acgataataa aaaagcggat actagaacaa tcaatgttgc
    25381 agttgaacct ggatataaga gcttaactac taaagtacat attgtcgtgc cacaaattaa
    25441 ttacaatcat agatatacta cgcatttgga atttgaaaaa gcaattccta cattagctga
    25501 cgcagcaaaa ccaaacaatg ttaaaccggt tcaaccaaaa ccagctcaac ctaaaacacc
    25561 tactgagcaa actaaaccag ttcaacctaa agttgaaaaa gttaaaccta ctgtaactac
    25621 aacaagcaaa gttgaagaca atcactctac taaagttgta agtactgaca caacaaaaga
    25681 tcaaactaaa acacaaactg ctcatacagt taaaacagca caaactgctc aagaacaaaa
    25741 taaagttcaa acacctgtta aagatgttgc aacagcgaaa tctgaaagca acaatcaagc
    25801 tgtaagtgat aataaatcac aacaaactaa caaagttaca aaacataacg aaacgcctaa
    25861 acaagcatct aaagctaaag aattaccaaa aactggttta acttcagttg ataactttat
    25921 tagcacagtt gccttcgcaa cacttgccct tttaggttca ttatctttat tacttttcaa
    25981 aagaaaagaa tctaaataaa tcatcgtcac actcataact taatatattt tttattttaa
    26041 attttattta acctatgtca tagatatttc ataatctata acataggtta tt
//
LOCUS       DAKTGS010000023        25712 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.023, whole genome shotgun sequence.
ACCESSION   DAKTGS010000023 DAKTGS010000000
VERSION     DAKTGS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.023"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(121..1029)
                     /gene="sucD"
                     /locus_tag="O0286_000823"
     CDS             complement(121..1029)
                     /gene="sucD"
                     /locus_tag="O0286_000823"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCY0169210.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            complement(1051..2217)
                     /gene="sucC"
                     /locus_tag="O0286_000824"
     CDS             complement(1051..2217)
                     /gene="sucC"
                     /locus_tag="O0286_000824"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCY0169211.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            complement(2326..3093)
                     /locus_tag="O0286_000825"
     CDS             complement(2326..3093)
                     /locus_tag="O0286_000825"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCY0169212.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            complement(3077..3961)
                     /gene="ylqF"
                     /locus_tag="O0286_000826"
     CDS             complement(3077..3961)
                     /gene="ylqF"
                     /locus_tag="O0286_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCY0169213.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            4362..6968
                     /gene="yfhO"
                     /locus_tag="O0286_000827"
     CDS             4362..6968
                     /gene="yfhO"
                     /locus_tag="O0286_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCY0169214.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            complement(7212..7562)
                     /gene="rplS"
                     /locus_tag="O0286_000828"
     CDS             complement(7212..7562)
                     /gene="rplS"
                     /locus_tag="O0286_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCY0169215.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(7665..8402)
                     /gene="trmD"
                     /locus_tag="O0286_000829"
     CDS             complement(7665..8402)
                     /gene="trmD"
                     /locus_tag="O0286_000829"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCY0169216.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            complement(8402..8905)
                     /gene="rimM"
                     /locus_tag="O0286_000830"
     CDS             complement(8402..8905)
                     /gene="rimM"
                     /locus_tag="O0286_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCY0169217.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            complement(9093..9368)
                     /gene="rpsP"
                     /locus_tag="O0286_000831"
     CDS             complement(9093..9368)
                     /gene="rpsP"
                     /locus_tag="O0286_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCY0169218.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            complement(9803..11170)
                     /gene="ffh"
                     /locus_tag="O0286_000832"
     CDS             complement(9803..11170)
                     /gene="ffh"
                     /locus_tag="O0286_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCY0169219.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            complement(11196..11528)
                     /locus_tag="O0286_000833"
     CDS             complement(11196..11528)
                     /locus_tag="O0286_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCY0169220.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            complement(11515..12765)
                     /gene="ftsY"
                     /locus_tag="O0286_000834"
     CDS             complement(11515..12765)
                     /gene="ftsY"
                     /locus_tag="O0286_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCY0169221.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            complement(12765..16331)
                     /gene="smc"
                     /locus_tag="O0286_000835"
     CDS             complement(12765..16331)
                     /gene="smc"
                     /locus_tag="O0286_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCY0169222.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            complement(16478..17209)
                     /locus_tag="O0286_000836"
     CDS             complement(16478..17209)
                     /locus_tag="O0286_000836"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCY0169223.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(17325..17558)
                     /locus_tag="O0286_000837"
     CDS             complement(17325..17558)
                     /locus_tag="O0286_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCY0169224.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            complement(17801..18535)
                     /gene="fabG"
                     /locus_tag="O0286_000838"
     CDS             complement(17801..18535)
                     /gene="fabG"
                     /locus_tag="O0286_000838"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCY0169225.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            complement(18528..19454)
                     /gene="fabD"
                     /locus_tag="O0286_000839"
     CDS             complement(18528..19454)
                     /gene="fabD"
                     /locus_tag="O0286_000839"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCY0169226.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            complement(19447..20433)
                     /gene="plsX"
                     /locus_tag="O0286_000840"
     CDS             complement(19447..20433)
                     /gene="plsX"
                     /locus_tag="O0286_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCY0169227.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            complement(20438..21010)
                     /gene="fapR"
                     /locus_tag="O0286_000841"
     CDS             complement(20438..21010)
                     /gene="fapR"
                     /locus_tag="O0286_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCY0169228.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            complement(21213..23273)
                     /gene="recG"
                     /locus_tag="O0286_000842"
     CDS             complement(21213..23273)
                     /gene="recG"
                     /locus_tag="O0286_000842"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCY0169229.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            complement(23463..25109)
                     /gene="fakA"
                     /locus_tag="O0286_000843"
     CDS             complement(23463..25109)
                     /gene="fakA"
                     /locus_tag="O0286_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCY0169230.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            complement(25124..25498)
                     /locus_tag="O0286_000844"
     CDS             complement(25124..25498)
                     /locus_tag="O0286_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0169231.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
BASE COUNT         7687 a         5060 c         3451 g         9514 t
ORIGIN      
        1 ttaacttgtc tttccaaaat atgaaaagtg tactaaaatt catcgcacga caaatagccc
       61 atttccgata cttttataaa gtatggaatg ggctatagcc atttatatca tcttttaact
      121 ttatttatta acagttaata atgattcata aatacctgct tctttagcag cttcaattaa
      181 tgttgaacca atttctgaag gtgttgccgc tgttttcaca ccacaactat ttaatgtttt
      241 aattttctct tcagcagtac ctttaccacc tgaaatgatt gcaccagcat gtcccatacg
      301 ttttccagga ggtgctgttt gtccaccgat aaagcctaca actggttttg tcatattcgc
      361 tttaatccat tcagctgctt cttcttcagc cgtaccaccg atttcaccaa tcataacaac
      421 tgctttcgtt tcgtcatctt cattgaatgc ttttaaaaca tcaataaagt ttgttccgtt
      481 gactgggtct ccaccaatac caacagctgt agtttgacca ataccttctt cagtcaattg
      541 gtgcactgct tcatatgtta atgtacctga acgagatact acaccaacat gacctttttt
      601 gtgaatatag ccaggcataa taccaatttt acattcatct gctgtaatca cacctggaca
      661 gttcggacca actaaacgtg tttttctacc ttgtaagtag cgtttaactt taaccatgtc
      721 taatacagga atatgttcag tgatacaaat aaccatatct aagtctgcat cagctgcttc
      781 taaaattgag tctgcagcaa atggtgctgg aacgtaaatg actgaaaccg ttgccccagt
      841 ttcattttta gcttcttcaa cagtgttgaa aacaggaacg ccttcaacaa cttgaccacc
      901 tttaccaggc gtcacacctg ctactatttt cgtaccataa tcaagcattt gttttgtatg
      961 gaaaagggca gtagaccctg taataccttg taccattact ttagtattct tatctataaa
     1021 tacactcatc ttagtgctcc catcctttcc ttatgcttct ttgactagtt taacaatttt
     1081 ttgtgcacct tcagccattg ttgctgctgg ttcaattgct aatcctgagt cttttaagat
     1141 ttttttacct aactcaacat ttgtaccttc taggcgtaca actagtggta aagttaaatc
     1201 tacttctttt acagcttcaa cgataccttc tgcgataaca tcacatttca taatgccacc
     1261 gaaaatgttt acaaaaatac ctttaacatt ttcatcacct aaaatgattt taaatgcttc
     1321 agttactttt tctctagtag cgcttccgcc tgcatctaag aaattggctg ggtttccacc
     1381 gaaatgatta atcgtatcca ttgttgccat ggctaaacct gcaccattaa ccatacatcc
     1441 gatgtcacca tctaatgcaa tgtatgataa atcatgttta gacgcttcaa tctctttcgg
     1501 atcttcttct tctaaatcac gtaattctac aacatcttta tgtctgaata atgcattatc
     1561 atcaaaatta attttagcat ctaatgccaa tacatcacca tcagctgttg taactaatgg
     1621 gttgatttct acgattgaac aatctttttc aatgaataca ttataaagtg ctaataagaa
     1681 tttagcagct ttgttaacag attctttagg aatattaata ttaaacgcaa ttcgtcttgc
     1741 ttggaatggt gaaagtccga ttactggatc gatagtttct ttgaagatct tttcaggagt
     1801 cttcgcagca acttcttcaa tctcagtgcc cccttcttca gacgccatca atgttacttg
     1861 gtcagtcgca cgatcaataa cgaatccaac gtaatattct ttttgaatag cacaaccttc
     1921 ttcgatatat aaacgcttaa tttctttacc ttctggacca gtttgatgtg tcaccaaagt
     1981 tttccctaat aattcttttg catatgtttc tacctcagat aaagatttag caatttttac
     2041 tccgcctgct ttacctctac ctccagcatg aatttgtgct tttacaacat aaacatcaga
     2101 atttaattct tttgctttct ccaccgcttc ttcagcagta aatgctactc gtccttctgg
     2161 aactgcaacg cccattgaac gaaatatttc tttaccttga tactcgtgga tattcatctt
     2221 ccatcctcct gttacttagg ttaagttccc ttacaattat aaaaaatgta agcgctattg
     2281 taaacttaaa tgctactttt ttatcattta attgaatttt acgatttaca gtaacgattt
     2341 tataggttca aagctttttc tatgctcttt cataatgcca atatcatcga ttgctagtaa
     2401 atgttgtttg gtaccgtaac ccgcgttttt ttcaaaacca tattcaggat aatctttaga
     2461 taactgtgtc atataatcat cacgaaaaac ctttgccatg atacttgcag ctgcaatgga
     2521 cacacttctt gcatcaccct tgattaaaga tacttgaggc agtgcattat caagcgtcat
     2581 cgcgtctatc aataaatgcg ttggttgtac tgataatcca tcaatagctc gctgcatggc
     2641 gatttgagta gctttataaa tattaaattc atctatttct tcagctgtcg cgatcccata
     2701 tgcaaaagca gtaacttcat tttttagtgc ttcatttaat tctagacgtt tcgtaacagg
     2761 tacttttttc gagtcatcaa ggcccaaata attgtgattt gaatttaaaa ttgttgcgca
     2821 tgcaacgact ggacctgcta aaggtcctct tccaacttca tcaatcccac aaataatagc
     2881 attaggatgc tcttttaata tttcattttc aaagtaagtc atttcaacat acttttcttt
     2941 taaagcttgt tctttttcta acgcttttct gcgcctagct atggcatttt gaacaccttt
     3001 tcgctcatct aaaaagcatt catgattttc taattcttct attgtattaa ccgcattaat
     3061 caactgcgta acttctttaa ttgttagcgt catttgctaa ttcctcagtc atatctttaa
     3121 aaatatcaaa acaataattt cctattttag catttcgaat atcataaata atcagttcaa
     3181 tgactgcttc gtaatcaatt tcattaccac gtcgaattaa gccacgtttt ttccctatcg
     3241 catcaaacca cgctatgatt tctgcatctt caggaacttc aatattataa tgtgacttta
     3301 atcgcgctaa atcattttga attaaaaagt ttaatccata gatggcaact tcatctaagt
     3361 gcacaatact atcttttatc gcaccagtta aactcaactt cttaccgact tcttcatctt
     3421 caaatttagg ccaaagtatc cctggtgtgt ctaatagttg taatgcatta ccaactttaa
     3481 tccattgttg ttgtttggtc acacctggtt tattaccagt ctgcgcaata ctacgctttg
     3541 ccagtttatt tattaatgtg gatttaccaa catttggaat tccaacgatc attgctctta
     3601 tcgctctagg tttaagtcct ttcgcttttt cgcgttcaaa tttttcagca gtcgccttaa
     3661 ttgctgcagc ttccactttc tttaaatttt taccgtgctt agcatccact gatacaggat
     3721 agtatccttt atcaataaaa aattgttccc attttgacat ctcatttaaa ttagacatat
     3781 cttttttatt taatataaca acacgtggtt tttggttaat aacttcatct atcatagggt
     3841 ttcttgaact atatggaatt cttgcatcta ctagttcaaa cactacatct acttttttta
     3901 attgttcact tacttctctt ttggctttcg ccatatgtcc tggataccat tgaataacca
     3961 ttcaattcac cttactttcg tcaattcttt tctcattatt aaaatctata acctgttaat
     4021 tatagaacaa attcattaat tcaactatat ttattttaac attgtcacac ttcttaacaa
     4081 tgctttagat ttaggtataa gtgattattt caagttttct acaattaaaa atcatcattt
     4141 gttaaatcta agttacttat taagtgacat tcatctttat cagtatgaca tttttattat
     4201 tcaatatatt tgtttaatga taaactgaaa atgatccaaa gtttaagtaa ctatgataag
     4261 gtatctgttc cacagtcatc ttcgtcttta cctatcaaga acctcattaa ctttagtttc
     4321 agttaaactt aattttaaat ttgaaagttg aggataatgt tgtgttaaaa aagtggctaa
     4381 attcaaacgt caaacaattc tttgttataa ctttcattag tgtaatatta acgcttattt
     4441 tattttctac tcatatctat gattatattg tgaatggtac tgtttttagc ggggctggag
     4501 atggattccg tcaaatgatg ccatttcaaa tgtatttgta tgaacatcta cgtagttttt
     4561 ctagtttata tgatgcatcg tttggattag gtggcgatta tatgaaagga ctatcatatt
     4621 attattcgct gtcaccttta atgtggctaa attttctatt cattaaaata ggagaaacgg
     4681 ttggtatatt taatccgacg acaatacatt tttggccgac aaaccaactt attatggcta
     4741 tgatacgagc tatcataaca tttgtcgtga ccttctactt atttaaaata ttacacttta
     4801 aacgctcagc aaatatgatc gctacgattt tatacggcat gtcaactgtc gttatatact
     4861 ttaattttac ttggtcattt tatggaaatt tattatattt attgccatta tcgattcttg
     4921 gtttggaaag atattttcaa caacgcaaaa tcggtatttt cattgttgcg atagccttaa
     4981 cactatttag caatttttat ttcagttatt atcaagctat tattataggt tgctactatt
     5041 tatatcgact cattttcact tacaaatatg acattgtctc tagaacacaa aaattaattt
     5101 gcgtcatatc tgctacagtt ttgagtgtgt tatcaagtgt atttggttta ttcactggca
     5161 tttctgcgtt tttggaaaat gacagaaagc aaaatcccaa tgttgatata ccgtttttga
     5221 caccacttga ttatcattat tttttcttta gcgatggatt ttatattacg atttcaattc
     5281 ttactatcgt tgcattattg tcattcaaac tgtatcgttt ttacttttat agacttttcg
     5341 caatagtaac atggatatta tttataggtt cattatcaca gtatttcgac agtgctttta
     5401 atggtttttc atttccagaa aggcgttggg tgtatatctt agcactatca tcaagtgctc
     5461 tttgcggatt gtttattcaa catttatcaa cattaaatat gaaatattat ttaatcagaa
     5521 caatacccgt atgcatcatc gcaatacttt atgtattact atcaccgaca cacccacttg
     5581 cacttatagt aggtattatc ctgctaatag tgcttgccgt tattttaaaa tttagtttat
     5641 ggcgttataa aaaattaacc gttgcaatat tagtattaat cgttatgatt caacaaatcg
     5701 tcattttaga taacaacaaa aacatggcaa tcaaacctta tcaacaatca ttatcaacgt
     5761 tgaaacaaca tgattaccat agtaactatg taaaccagct tataaaaaag ataaatcaaa
     5821 atgcaacagg ctcatttaat cgcattgatt atatgtcaga ctatgcatta aattcaccat
     5881 ttatatatca ttataatggc atttcattat attctagtat ttttaatgga gacattttaa
     5941 aatattatga caagacactc caaattaata tgccaatcga taaaaacagc acttatagat
     6001 tacttggcaa tcgtcaaaat ttactatcac tttggaatgt taatgatcga attagagtga
     6061 atcatgatga caacttacca tatggattta aaattaagtc tgaacacaaa gacaataaag
     6121 ttcgttggat tcattctaaa aataccatcc attatccaag tgcacatatt acaaataagg
     6181 tcttttccaa taaagaatta aaatctccat tagataaaga acaagcaatg ttgcaaggga
     6241 ttgtttctaa caatattaaa gatgttaata cacattttaa agccaataaa aatttactat
     6301 cagattcaac aattaaatta aatagtgcag cctggcaatc tcctacaaaa catttattac
     6361 aagttaaaca aaataatggt ggtctaactg tacagttgcc aaaatcagtt tctaatcaat
     6421 ttaaagattt gtattttgaa atggatttag aattactttc gccggataaa gctcatgatg
     6481 ttaaagtgaa tgaatataca caagaaagaa ataaactcac ttataaatat cgacgcgttg
     6541 taacacccgt aacgatacgc attaaagctc cagatagaat tagattatca ttgcctaaag
     6601 gtaagtatcg agtaaattta aaagggatat acggcgaaga ttataccacg cttaaagacg
     6661 cttcaaattc attagaagct gtcaaagtta gtaagacaaa gcatggttat actattacta
     6721 aaaataaaaa ttcatctggg tatattgttt tgccaacagc atataatcaa ggtatgaaag
     6781 cgacatcagg tgatcaaagt cttaaagttg aacaagtaaa tggtgttatg accggcatta
     6841 aagcacctaa aaatataaca aagattcaat tgagctatac cccaccatac tattatttac
     6901 ttataacaat tactatattt ggcattatat gtagtattat tttcacgaga tgggcaagac
     6961 aaaaataatg ttgctacgca tatatttaat aaatctacta agtccttttc aatggcactt
     7021 agtagatttt tgtttatata accattttga gcaaacacgt tatgaataga gaaggaataa
     7081 acaattacat tatcaacttt acattaacta aacagcaaaa aaagccatct atatcactcc
     7141 ataaaagtga cctagatggc tttattgttt gagtcgaatt tgactcatac atagctttgt
     7201 ttaaatgctg attaacgaat ttcttggatt ctagcagctt taccacgtaa actacgtaag
     7261 taatataatt tagcacgacg tactttacca cgacgtttaa cttcgatttt ttcaattttt
     7321 ggtgtgtgta atgggaatgt acgttccacg ccaacacctg atgaaatttt acgaactgta
     7381 aacgtttcag aaacgccacc gccacgacgt ttaattacaa cgccttcgaa tacttggata
     7441 cgctcacgag taccctcaat gatacgtacg tgtacacgta aagtatcacc aggacggaaa
     7501 cttggtaagt ctgtacgtaa ttgtgattta gttactgctt cgattaattt gtgatttgtc
     7561 attataaatt ctcctcttca acctatgttc ttgcctcgac aaatatatag cagcggatca
     7621 tagtgatttt agtgtttcca cactcagatt atgttagcac aatactaacc ttttttcaat
     7681 cctattttat atctttctaa tatttgctta tctgcattag ttaatggata tttttcaatt
     7741 aagtcaggtc ttttattata tgtgcggatc aacttttgct catgtctcca tgcatcaata
     7801 ttggcatgat ttccagacaa taaaacatct ggaactgtta gacccttaaa ttcacgcgga
     7861 cgtgtatatt gcggaaactc taataaccca tctgaaaatg aatcgtcttg atgtgactgt
     7921 tcattaccta aaacacctgg aatcagtcta acaatagcat cagtcatggt catcgctggc
     7981 aattctccac cagttaaaac atagtcaccc attgatattt catctgtgac aagatgtgtt
     8041 cggatacgtt catcgtaacc ttcataatgt ccgcatatga aaacgatgtg gtcggccttg
     8101 cttaattcaa cagctttctg atgtgaaaat ggctcgcctt gtggacacat taaaataacg
     8161 cgtgtttgtt ctgtgacatc taagtcttcc atcgcattaa aaacaggttc aggctttaac
     8221 accatacctt gtccgccacc atacggataa tcatctactt ggttgtgctt gttaattgca
     8281 taatctctaa aattaaccgt attgatttgt aatttattgt tttcttgggc acgtttcata
     8341 attgaatgat ttaaaacacc atcaaacatt tcaggaaata aagttaaata atcaattttc
     8401 attaatccaa caatccttcc attggcgtga tgataatttt tttattttca acatccactt
     8461 cttttacaac atcagcaata taaggaatca aatattcttt agatccttta atcacccaca
     8521 catcattcgc acctgtttca aatatattaa ttacacgacc tattggtgtt tcttgatcat
     8581 caaaaactgt acatcctata atatctgaat aataaaattc attttcctca agtacgatat
     8641 cttcatgatc acgctcttga taaatagaac tcccttttaa atgctcaata tcattaatat
     8701 tattaatacc ttcaaatgta agcatatgaa gccctttatg cactctatgt gacttaacag
     8761 tatattcaag gtcgttattg ttatgcacaa ctgtcagcac ttgaccgggt tgaaaacgaa
     8821 cgtctgtaaa gtctgaattg gattttactt taatttcacc tttaatacca tgtgtgttaa
     8881 caatttgacc aacttcaact ctcattctat tacctccaac tctatgcttt gaacacttct
     8941 attttaattg aaaataattc aaagaaaaag ggcaccatta tggtacccaa tacgttctat
     9001 attactaaca tgtgatattc ccaacgtatc attgagtaat aactgtcagt gtaaacgagt
     9061 tattcttatt gttagaacaa ttttacgcta aattacttag ctttcttttg ttcgtcaaat
     9121 tttttcataa taccttcttt tgataagata ttgtgaactg tatcagttgg tttcgcacca
     9181 tcatttaacc attttaaagc taacgcttcg tcaactttaa tttctggagc attagcgctc
     9241 gttgggttat aagtaccgat ttgttcgatg atacgtccgt cacgtggaga acgagcatct
     9301 gctactacga tacgatagaa tggatttctt tttgaaccta aacgtgttaa acgaatttta
     9361 actgccattt ataaaatctc ctttaagtca tgttttaatt tttattttct acaagaaata
     9421 atattaacag gtgtatgaat gtttgtaaag agttttttct ttaccaggaa aattattgaa
     9481 cctttttctt acaataataa ctatgtgatt actaaattag actgttaata ggttataaaa
     9541 attcatagta aatttcacca cctacgtgtt aagttaattt atctcgtaag gacatgaatg
     9601 gtaccacttc taccgcacac gttcccccaa tataacttat taatattagc aacaataacc
     9661 tcatgttcta gcaaatcagc aatttcagtt tttaattatt attgtgtatc taaaaatatg
     9721 caccccataa agttatattc tttagtataa ctttcgggat gcacatctca ttgcaacaaa
     9781 aatcagcgtg ttatattaaa cattaaaacg gtaaattcat accttttaac atattttgca
     9841 tttgattgcg tttacctttt ttacctttac cgccaccagt gaactgtttc atcattttct
     9901 tcatatcgtt aaattgtttc atcaaacgat tgacttcttg taatgaacga ccagaccctt
     9961 tagcaatacg ctttttacgt gatacattca atgtgtctgg attgtttctt tcagccggcg
    10021 tcattgactg gataatcgct ttaatatgat caatttgctt ttcactcata ttaagcttat
    10081 ctagcccttt cattttattc atacctggaa tcattttcat aatatcatcc agtggtccta
    10141 gatttttcac ctgatcaagt tgttctaaaa aatcatctaa agtaaacgat gactcacgca
    10201 tctttttctc taaatctttt gctttttctt gatccacatc ttgttgcgct ttttcaatta
    10261 aacttaacac atcacccata cctaaaatac gtgatgccat acgttcagga tggaatagct
    10321 ctaaaccatc taacttttca ctcataccaa caaatttaat tggtttttgt gtcaccgaac
    10381 gaatagataa agctgcacca ccacgtgtat caccatctaa tttagttaag gtaacacctg
    10441 tgacatcaag ttgatcgtca aaagattctg caacattgac agcatcttga cccgtcattg
    10501 aatcgacaac taacataatt tcgtttggtt tagcaatgtc ttttacttct tttaattcgt
    10561 tcatcaatgc ttcatcgatg tgtaatcgac ctgctgtatc aatgattaca aagtctaaat
    10621 gttcttcttt agcatgtttt aatgcattag ttacaatttg ttgtggcttt acttgatctc
    10681 cttcactgta tacaggaata tcaatttgtt tccctactgt ttgtaattga tttatcgctg
    10741 ctggacgata aatatctgct gcaactaaca taggtttttt gttgtatttt ttacgcatca
    10801 ataatgctaa tttacctgca gttgttgttt taccagcacc ttgtaaacca accatcataa
    10861 caacagtagg tggtttattt gacatattaa tcgacgtatt ttctccaccc atcaactgcg
    10921 ttaattcatc ttgaactatt ttaataactt gttgccctgg tgttaatgat tgcattacat
    10981 cggaacctaa tgcgcgttct gatactgttt taataaattc ttttaccact ttaaagttta
    11041 cgtcagcctc aagtaacgct aatcttactt cacgcatcat tatctttata tcagcttcag
    11101 taagtttacc cttaccacgc attttttgca tcgtcgcttg caagcgttct gataaccctt
    11161 caaatgccat atcgatgtcc tccctcaatt tatatctatt ctaagtcttc taattgttga
    11221 atataacgtt gtatttgttc tggattactt aaatgttgtt tcatttcatc atatatttct
    11281 cggcgttgct caaatttctg gtataattcc aatttctttt cataatcttc aactaaatcg
    11341 ccagttcttc ttatattatc ataaactgct tgtctactca cattaaaagt atctgcgatt
    11401 tcacttaaag aataatcttc aagataaaat aattccaaat aattacgttg tttattcgtc
    11461 aataaggatt gataaaaatc aaacaaataa ttcattcgta acgttttaac taaatcattt
    11521 tgacccatga ttatcgtcct tttcttctgt tacaatttga tcattttcaa ctgttgttat
    11581 ttcttcattt tgttcaatca tatcagcgaa taagccgtag acataacttt cagggttaaa
    11641 tggttgtaag tcatctaatt gctcacctaa acctacatat ttaactggga tgtgcaattc
    11701 attacgaatg gctaatacga taccaccttt ggctgtacca tctaatttcg ttaatacaat
    11761 acctgtaaca tttgttactt ctttaaagtt tctagcttgt gacaacgcat tctgaccagt
    11821 tgtagcatct aaacatagta atgcttcatg aggcgcatct ggcactgctc gattaattac
    11881 acgcttaact ttttctaatt cttgcattag atttgtttta ttttgtaaac gtccagcggt
    11941 atcacagatt aaaatatcaa cacctttgtt tttagcggca ttaatcgcat catacataac
    12001 agcagctgga tcagaacctt cactttggct aattacgtct acaccaacac gttcgcccca
    12061 aactttcaat tgatcaatag cacccgctct aaaagtatcg cccgcagcta acattacttt
    12121 tttaccttcc attttatatc ggtaagctaa ttttccaatt gttgttgttt taccaacacc
    12181 attcacacca accattaaaa tgacatttaa acgaccatct tctaagttca tagcttctga
    12241 attatcatct tcttgatggt aaatctctac gattttttca acaatgactt cacgcaaatc
    12301 ttcagtatct tgaatattac gtcgttgtgc ttccatacgt aattcttcag ttaacgtcat
    12361 cactgtatta aaaccgacgt ctgcagtgat taacatttct tctaaagctt caaaaaagtc
    12421 ttcatctact ttacgatatc tcgctatcaa attatttaat tgttcttgga aattttgacg
    12481 agatttttcg agtcctgctt taaatttagc acccattttt tgagcttcaa tttcttcaaa
    12541 atcttcaatt gatattaagc catcgtcatc aaaatccgct tcactcaact tgcgtggctt
    12601 ctttttcact tcagcatttt ctgctaaatc atttgcgtcc tgcgttgaac cttcagaatg
    12661 tgtatcttct aatttgtctt gaccttgttc ttctgttaag gatttaactt cttcattttc
    12721 tttatttgtt gcaaacttat cttttaagcg tttaaaaaag ctcattattg ctcctccttc
    12781 aacacatcat ctattgtatt taaattcaca ctcacaagtt tagtaacacc tgattcttgc
    12841 attgttacac cgtataacct atctgcaaat tccattgttc ctttacggtg tgtaataaca
    12901 atgaattgtg tttcgtctga taactcattt aaatattttg cgtatctaat aacatttgct
    12961 tcatctagtg cagcttcaac ctcatctaat ataacaaaag gtgcagatct tacttttaaa
    13021 attgcaaata gtaaagcaat agcagttaat gcacgctcac caccactcag taacgataaa
    13081 tgttgcaact ttttacccgg tggttgtacc acaatatcaa taccagctgt taaataatcg
    13141 gcttcagtta attgcaattc tgcatcgcct ccaccaaaca attgtttgaa cacagctgta
    13201 aaatgtcctt gaatagcatg gaaagtttct ttaaatcttt cagtaacctc ttgatccatt
    13261 tcacttataa tttgctctaa tgtttcttta gctttacgaa gatctgtacg ttgttcactt
    13321 aaaaatgtat aacgttcatt taactcttca aattgttcaa ttgcatttaa gtttacagga
    13381 cctagttcat caatcgacat cttcattaac ttaacttttt tacgtaatgc gtcaatcgat
    13441 tcatcactcg tatattcaga tttcgcacgt tcaacagtca attgatattc atcatttaaa
    13501 tgatctatcg catgatgaat taatacatct agctttgatt gttcagcttt aatatcttgg
    13561 tagtgatttt cgatagctaa aatatcttgg tgacaaactt gtagtttagc ttcttgcgca
    13621 tcgatttgtt cattcaactc aatacgttgt tgtttcaatt tatctaattc atctgataag
    13681 cgtgttctcg tttcttgttg accattaatt tgatctttaa tattttgaaa agcttgttcg
    13741 cccatcactt catccgaatt aaagaatgca attttttctt taacatcttt taattgatgt
    13801 ttagtttgtt gattttgatt atttaatcga tctattgtct gttgttgtgt tttaatacgc
    13861 tctttaacca cagcaagatc agattgtttc tgatgtaagg tttgttgtgt tttagtaacg
    13921 ctttccttac cttctttaga aagttttgtg tagcgttcaa tttcatcttc tagtcgtttt
    13981 aaagatgctt taatactttc tagataagtt tctttttcac tcaaagtttg tcgacttttg
    14041 tcactcgtat aaccatcatt tttttcaaat tcgaattctt catgatcatt ttttatttgt
    14101 gtttcttgtg tagttaatct atcgagctcc atttcaaaat gatgcacttg ctctttaagt
    14161 gtattatgct tttgactttt ttcaaaatac agttcactta attgatcact ttttatcttc
    14221 aactctttaa attgttgttc aaatgattct gtttgacgca agtaatcttc taattggtgt
    14281 ctcattgttg tcaactcgtc tttttgagac agaatacttt ttgacttacg agcgccacca
    14341 ccagtcatag aaccaccagg atttacaata tcaccttcca aagtaacaat acgagttcga
    14401 tatttaatcg cacgtgccaa ttcatttgca tgctttaaat gatcaacgat aatcgtatta
    14461 cctaataaat tcccgataat attttgatat tctggtgcta ctttaactgc ttccgaagcg
    14521 atactaataa atccgtttgc ctctttagca atagatttaa tatcagtcgc taccactcta
    14581 ctctgtataa catttaatgg taaaaacgtc gcacgaccta aattacgttc ttttaaaaat
    14641 tgaatagcct ggcgtccatc tttttctgaa tctacaatga catgttgtaa tgaagcacct
    14701 aatgctgttt caattgcctg agttaattta gatggcacat caataatttc cgcaactgca
    14761 ccataaatac cctttaattc tttattttta gctttcaaaa tatgtttgac gccattgaaa
    14821 aaataagtat attcttcctc ttgcgttgcc aaactatcaa tacgtgtttt cattttttcg
    14881 gtatatcgat atgcttgata caatttctct tcgtattcat tttgtgcttt ttttgtatca
    14941 gtgaggtctt tttctatatt tttaatttct ttatctacag cagaaagttc tttgttggtc
    15001 tgttgatatt ctttttttgt cgtttttatt tgaccctgaa tatctttcaa ttgctcaaaa
    15061 acttcaacta atcgagaatc tagtcttgat tttttagcct cattctcttc tatagtatgc
    15121 tttaaaaaac gaatatcatt gttaacatct gattgctctg acattaatgt atagtattcg
    15181 tttttaattt cttccaattt ttcatcatgt gcttcgtctg aaacatatag ttgttcttca
    15241 agttcacgaa tgacagcatt gagttctttt tgtttacttt tcagagactt ataagtatct
    15301 tgagcttcag aaatctcatt tgatatattt tctaaaagct ccattaaatt ttcttgttct
    15361 tcttcatatc gtgcatttgt ttcagattga tttttcttac gttcttccaa aacatttaat
    15421 tgtcccgtat atttttcaaa ggcttccgta gcttttacta attgataatt aagcgattca
    15481 acatcattat caagttgatg acgtttacct ttatattgtt gaatttgttg gcttaaacgt
    15541 tgcttgtcag cttctttatt tgcttgttgg ccttgtaaat catttaaacg ttgatctaat
    15601 tgtctattgt catttgtata ttgatcaata tcgtgcactg taactacaat gtcactatgt
    15661 ttcatttgat gtgaaagtgt cttatattct ttagctatag ctgcctcctc ttttagaggt
    15721 tctacgcgac cttccaaatc atataaaatg tcttctactc tcgttaaatt atcttctgtt
    15781 tggtcaagtt tatttaatga ttcagcctta cgttttttat atttaagtac accagccgat
    15841 tcttcaataa tttgacgtct atcaattggt ttagcattta gtatttcatc aactctacct
    15901 tgcgagataa tgctatacgc ttcttttccc aatccagaat ctaaaaataa atcggcaata
    15961 tcttttaatc ttgcacggtc attatttatg tagtactcac tttcaccact tcgatacaat
    16021 cttcttgtta caataacttc gttttcatca acactgagct ttttagaatg attatctaat
    16081 cttaactgta cttcagcata attttgagct ttgcgatgtt ctgcacctga gaagataata
    16141 tcttccattt ttgagccacg taatgattta gccgattgtt cacccaacac ccatttaata
    16201 gcatctgtaa tattactttt accgcttcca tttggaccaa caattgcagt tacaccttta
    16261 tcgaattgaa cattggtttg atctgcaaaa gacttaaatc caatggcatc tattgatttt
    16321 aaataaacca tcctaaactc cttatcacaa ttttaaagaa cacatgtata cgatatttta
    16381 gtatttgtat attaaatttc gaagttgttc cataaaatat taaaaattct tccttaaatc
    16441 ctaaatcgaa cacctatatg tcgtatcata taaatttcta tttaatttgt tttaattgct
    16501 tataggcact ttcagcagca cgttgttctg attctttttt cgttttccct ttaccttcag
    16561 ctattgcttc cccttgcaga ataacttctg aagtgaatag acgatgatgt gccggtccct
    16621 cttcttttat taaattatag gttacatcac ctttattttg ctggtgcaca tattcttgga
    16681 attgtgtttt aaaatctacc acgcctaata actcattttg ttctacatgt gggaaaatga
    16741 ctttctcagc aaatttccaa actatatcta gtccttgatc caaatacaat gccccaataa
    16801 atgcttcgaa tgcatctgat attaatgatg gtcttgtacg tccccctgtt ttctcttcac
    16861 ctttaccaag taaaatcatt tcgttcaatc caattttatt cgcaaatatt acaagtgagg
    16921 gctcacatac aatagtggca cgcatttttg ttaaattccc ttctggcaag ttgggatgtt
    16981 tatcaaataa atatcgtgaa accgtcaatt ctaataccgc atcacccaaa aactctaaac
    17041 gctcattatg gtctaaacga ttcatattaa aatcattaat aaaactcgaa tgcgaaaatg
    17101 cttgttggta taaatcaata ttttgataag taaagcctaa ctctgtcatt ttagtatcaa
    17161 agcgctttct aaaacgatta actatctcac ttttcttttg tttagacatt attttatctc
    17221 ctttatgtgt tgctcttgaa tcatctttaa ttttacgtta aaactattga taattaaaga
    17281 aaaaaactga gtcgacaata ctgacgaccc agatgtaaga tttattattt ttcaagactg
    17341 ttaataaatt taacagcatc accaacagtg ttgatttttt cagcttcttc atcaggaatt
    17401 tcagtaccaa actcgtcttc taattccatt actaattcag cgatatcaag tgagtcagcg
    17461 cctaaatcat ctttgaaaga tgcatcttca gttactttat cagcgtctac acctaaacgg
    17521 tcaacgatga tatcttttac tttatcgaaa ttttccacgt cgattcacct cctttaaaaa
    17581 tgcctataca tagactacta tattttccca ttttaaattc taaaaaacaa gtagaaattt
    17641 tagaaacact tatcaattta ttgattagtg tttcttaggg agaaattctg gaaccttatc
    17701 taggttgctt agaatttccc ccgcaaagtt gactaggtca gacaattctc cattggatga
    17761 ccagtcaacc actgcgtcaa tgagctttag ctcaaatata ttacatgtac attccaccat
    17821 ttacatggat tgtttgacct gtaatatatt ttgctttgtc tgatgctaag aacgctactg
    17881 tattagcaat atctgtgtct tgaccaaaac gtgctaacgg aatttgagtc aacatttgtt
    17941 ctttaagctc atcacttaaa gcatctgtca tatcagaaac aataaaacca ggtgcaactg
    18001 catttacagt gataccacga gatgctaatt cacgcgccgc agatttagtt aaaccaataa
    18061 cacctgcttt tgttgcaaca tagtttgctt gtcccggatt acctactgct ccaacaacac
    18121 ttgataaatt gatgatagca ccactacgtt gtcttaacat ttgtggtgtt gctttttgga
    18181 tacagttaaa tacacctttt aagtttgtgt caataacatc atcccactct tgttctttca
    18241 tacgcattaa taaattatcg cgagtaatac ctgcattatt tactaaaaca tctaaagaac
    18301 caaattggct aactacttct ttaatcattg ctttaacttc atcagcatcg gcaacatttg
    18361 cttgaatcgc aaaactgtca acacctttag ctttgatttc ttcgactact gcttcagctt
    18421 tctctttgct gcctgcatag tttactgcta cattatatcc ttcttctgct aattgtaacg
    18481 caatactacg tccaattcct cttgatgcac ctgttactaa agcactctta gtcattttca
    18541 ttccatcctt tcacatcttc taaagtttga attgatgtta acttaacatc tctatttatt
    18601 tttttaatta agccagataa aacttttcca ggaccaattt caataaaatg atcaacacct
    18661 tggtctatta gccattctgt tgagttaatg aattgtactg gtgaatataa ttgcttgacc
    18721 atattagatt taattacttc tttgtcagtt tcaccttgcg catttacatt ttgaactaca
    18781 ggaaacttag catcacgcca ttcaaattga ttaatgtaac ttgaaaaatc ttcttcaatc
    18841 actttcatta gcgatgaatg gaatggtcca gatactgcta aaggcatgac acgttttgca
    18901 cctaatgatt tacctttttc tactagctca tcaattaaag ctttgtgacc tgaaacaaca
    18961 atttgacctg ggcaattaat gtttgctggt tcaattattt tgtcatcaga tgataatgac
    19021 ttacaaattt catcgacttt atcaaaatct aatcccaata ctgcagccat gcttcctaca
    19081 ccagtaggaa atgcttgcgc cattaattga ccacgttttc taacaatttt aactgcatct
    19141 tcaaatgata atacgtcagc tgcaactaaa cttgaatatt cacctaaact atgccccata
    19201 gtaaaatcag gattcaaatt ttttagcgct gctaataatg ccgaactatg cgtcaataaa
    19261 gctggttgtg tgttttcagt ttcacccaat ttaccttctt catcagtaaa cattgtctct
    19321 aaaatatcaa agtctaatgt gttcgctgct gaagttaaaa tttcagttgc ttgatcattg
    19381 ttgttaaaca aatcttgcgc cataccaact ttttgggcac cttgtcccgg aaaaataatt
    19441 gctgttttac tcatttgatt cacctacagt ctctttcatt gtttgtacaa tattttgttc
    19501 tcctgcgatt ttcgcttgtc taattgcaga ataaaaagct ttagcatttg aactaccgtg
    19561 tgctttaact acagtacctt ccaatcctaa taatacggaa ccaccgtatt ctgagtaatc
    19621 catcttttta gcgaattcag ctaaatcttt cttcaatatt gcacctgcta atttattttt
    19681 agtactactc ataatcgtat cttttaacat tttaccgatt gattttgcag taccttctaa
    19741 atttttaagg accatgttcc cagtatagcc atcggtaact acaacatctg tatcgccatc
    19801 cattaatgtc ttcgcttcaa tattcccaac aaaattcaat gaatgatcat gatttaataa
    19861 ctcatatgat tttttcgtta aactattacc tttagctggc tcggttccta tatttaataa
    19921 tgagattttc ggattatcaa tacctctaat tttttgagca taaatatccc ctagttgcgc
    19981 atactgtaat aagtgttcag gtttagcatc agcatttgca ccaacgtcta aaaagacaaa
    20041 accttttcca tcaatcgttg gcaatgttac tactaaagcc ggtctagcta cacctttaat
    20101 acgtccaaca atgaataaac cagctgacat taaagcacca gtattacctg ctgacacaca
    20161 tccatctgct tcaccagatt tcacagcttc agccattttt accattgagc tatctttttt
    20221 acgtttaatc gctctaacag gctcatcttc catttcaatc ttttcagaac aatgtctaaa
    20281 ttcgattcgt tcatggttca gattatactt tttttcgtca ccgaaaagta taatttctag
    20341 atctttaaag tcttcaacag ccttttgtac ggcttctaat acgatatcag gcgcattgtc
    20401 gccacccatc atatcaattg ctaatttaac cataatttta tcctcgctta tcataaaaca
    20461 ttttaaaatt tcctttgaaa actaatgtat gtttaacata tgactttact tcgacgtaat
    20521 aatgttttgc agtttgattt acaactcgtg cttctgctct taccgtatca tttaatttta
    20581 ctttttcaat aaattgaatg ctactctcat gagttaaaac tgttggttgc ttaattagcg
    20641 caacacataa cgaatttgcc tgagcaaaca gcacatgacc acgcgcaatt ccagttttat
    20701 gaaaaacaga atccgatgta atatctaaaa ttgattgcgc tttaacattt ggattgactt
    20761 gaatcaaatc accaataaat tcttgttctt caatagaact tatttggtca taattctttt
    20821 cagcaactaa tttaatacgc ttccttaatt ctggtatgtt taaataagtg cgatctaaac
    20881 gaattgtttg tatactcact tgaaataagt cgcttagttc atggtctgtg atgaagggat
    20941 tgctatcaat ttgttgtctg attgcttctc tacgtttatc tttctttagt ttcaacgtct
    21001 cacccctcat tttttagtac ctagtcttaa acattcctca catatcataa cactttctat
    21061 ttaaccgact caacaaataa ctgtaatatt tagctattat ttagtcgaaa atttataaat
    21121 tgttaaaata ccactttaca tttaaatagt tcaagtgtca tatcactcaa ttaacgaagt
    21181 gttgcactca aattgacaaa tcagcatggc aattagtcaa aactacgatg taataaattt
    21241 tcttcaacaa aatgacgtaa atgttgatac gtattttcaa agaatacgcc agattgaata
    21301 agttcagctg cttcatcacg agcaacttct aacatacgat aatcttcaac taaattggca
    21361 actaagaaat ctggcaatcc actttgttta acaccaaaga aatctccagg accacgcatt
    21421 tctaagtctc gttcactcaa ttcaaatcca tccgttgttt gtgtcataat tgtcattctt
    21481 tcaattcctg tttctgtttt aggggatgca attaaaacac agtaactttg ctggtcactt
    21541 ctacctacac gaccgcgtaa ctgatgtaaa gttgataatc caaagcgatc cgcatcataa
    21601 atcatcataa aagttgcatt cggtacatta acacctactt caacaacagt agtagaaact
    21661 aaaacattta tctcatgatt actaaacttt tgcatgacct catctttttc atcggcagat
    21721 aacttaccat gcaataaccc tacacgggaa acaccataat actgttgtaa agactcgtac
    21781 aatgcgacaa cattttgaac atcttcgaga tgctcagaac tttctattag cgggcaaatg
    21841 acatatgctt gacgaccttt tttcaactct gaggtcattt gcatcaaaac tttatcgtat
    21901 tgctcatgct ttgcccaagt agtaatgata ggtttacgac cttttggtaa ttgtttaatt
    21961 gaagacacat ccatctcacc aaaaactgat attgctagtg ttcttggtat cggcgttgct
    22021 gtcataaata acacattcgt cattgcacct ttttctctta aaagctggcg ttgattcaca
    22081 ccaaatcgat gttgttcatc tgtaattact aaaccaacat tatggaaaat cacatcatct
    22141 tgaatcaaag catgggttcc aattaaacaa tcaatcgtac cattttcaag ttgttctaaa
    22201 agtatctttc gtttcttacc ttttactgac ccagttaaca atgcaacgtt catagaatct
    22261 ccaaataaag ccattaaact ttcagcatgt tgctctgcta aaatttcagt tggtaccatc
    22321 aatgctgatt gataaccagc agtttttaac gcatacatac aaattgcagc aactactgtt
    22381 tttcctgaac ctacatcacc ttgaagtaat cgatgcatac gtattggtgc ttttaaatct
    22441 ctaaaaattt cattaacact ggatttctgt gcttcagtta gttcaaaagg taaacgatca
    22501 ataaatgatt taacttggtc tatgtcataa tcaatttcaa ttgcttcgtc agatgacttt
    22561 tctaatctat ttagccattg catacgtaat tcgaataaaa acagttcagt aaatgcatag
    22621 gttctacgag cacgtaataa atcctcttta cttttaggat gatgtaatgt gttcaaagta
    22681 aagtccaagg tctctaattt atatttttct cttagttcat cagttaacca ttcatgaatt
    22741 gtcacatcat ttaacgcttg tctaatttgg tctcgtattt gcttttgttt aataccttcc
    22801 ttaatacgat agactggttc taattgaaca tctgcgtttt cttgagtttg tgtcccttgt
    22861 gaattaaaga aaaccctatt accagtaatt tcctgtttaa ccctattcca cttaccttta
    22921 acagttatag tttgatttaa ttcgattttc ttttttaaat acggttgatt gaaaaaaata
    22981 catttgacag caatattatt taccattaaa tgaacggtta attttgattt atttcttcca
    23041 aaaaatgcaa ctactggagc tgtatatact tgtccttcta tcgtaacgtt agattgatct
    23101 tctgcttgat tcaaatcaat cactgtatta tcttcatatc tagttggcaa ataaagaaca
    23161 agatcttcca ctgtatgaat atttagttgt tgcaatactt ctattttctt aggacctata
    23221 ccttttaatt gtaaaagaga atatggactt tctattaagt ttactttagc caaaataaat
    23281 cacctatgtt ttatttgatt ttacgtatca tttagtatgt tatttatttt atcatgacta
    23341 agttgaacat gacattgtta gcaactcgag aacgatactt ttaacccgca atatcactaa
    23401 aatagcaaaa taaaaaacta ccaactgatg atttatcatt ggtagttttt tattttaaat
    23461 ttttattcta ctgaaaagaa atattgataa attggttgtc caccttcatg aacttccact
    23521 tctacatctg gatattgctc ttcgatccag tttatcatgt tatctgtaac tgcttgctct
    23581 gcatcttgac caataatcac agtcaatatt tcactatctt ctgctaacat ctcatttaac
    23641 aactcagtaa ccgttgttaa ttgatcactt tggctgctta caatcttatc ttcaatcaag
    23701 cccataaacg cgtctttttt aatctcaacg ccatcaattt tcgtatcacg aacagcgtac
    23761 gttaatgaac cagatttaac gttatttact gaatcagcca tttgcgcttt attttcttca
    23821 agtgttgcgt ccacatcata ttggaatagt gcgcttatac cttgaggaat agatttcgtt
    23881 ggaataacaa cagcttctgc atcaacaata ctcgctgctt gttcacttgc cattaagata
    23941 tttttattat tcggtaaaat aattgcacgt ttacattttg attgttcaat gactttaacg
    24001 atatcttctg tagaaggatt catcgtttgt ccaccactaa tgatatgtgt ggcacccatt
    24061 gatttaaata tctctgaaat accttcaccc atagaaatag taataattgc tgtttcaacc
    24121 gtttccattt tcggtttagc tgtgtgctgt tcttttcgaa tcacttcacg gtgctgttct
    24181 ctcatatttt caaccttaag tttaattaat tcaccatatt gttgaccata attaaacact
    24241 ttacctgggt attcggtatg cacgtgaact ttcacaattt cttcatcatt aatgactaat
    24301 aaagaatcac caaattgact catatcttgc ctgaattctt gttcatcaaa ggctttttta
    24361 ttctttccaa aacgaaccat catttcagta caatagccat aaataatatc ttcagtatta
    24421 attacaccat ggaaatcatg ttcatcatgt acaaattcat ctttatctat ctttgcaact
    24481 ttggcttcaa ctttttcacc tttaagcgct tttaagaatc cttcgtaaac gcataacaaa
    24541 cctttaccgc cactatcaac aacaccaact tctttaagta cagctaataa gtttggtgtg
    24601 ttttcaagtg attcattggc ttttacaata atgtactcca ttaattctat acaatcttca
    24661 gtattatttg ctttttctat tgcagcttgc gcagcatctt ttgcaactgt aagtattgta
    24721 ccttcaactg gtttcataac agctttatat gccgtttcaa caccagcttg aaaactttca
    24781 gctaacaatt ttgaattaat ttcagattca ctttcaatat ttttacaaaa tcctctgaat
    24841 aattgtgaca agatgacacc agagttacct cttgcaccca ttagtaaacc tttcgagaat
    24901 gttttaccta attcgccgat atttttcgac aaattattct ctacttcttc gcgacctgaa
    24961 gtcatagtaa gattcatatt tgttcctgta tcaccatctg gcactggata cacattcaaa
    25021 gaatctacca aatctgcatt gttagataaa ttttgtgccc cttgtataat catatcggca
    25081 aataatttac cattaatttt gctaatcatt tcaagttgtc ctcctaagct ttcttgcctg
    25141 tattattcac acgtacacct tgtacatata tattgattga atttactgat acattaagtg
    25201 atttttccaa agtatatttc actgttgatt gtacattatt ggcaacttca gatattttca
    25261 caccgtaact tacaataatg tacatatcta tatccactac gccattattt tcagttactt
    25321 tgatgccttt agcataattt tcatgtccta gtatttccgc aataccatct ctaacttgtt
    25381 gtctagatgc catacctaca ataccataac attcaacggc ctttccacct acaaccgaag
    25441 caatcacttc gtttgaaata tcaattttac cgtaatcatt tgaaatctct aatgtcatat
    25501 ctttgtccct cctaaatttc aaagtttatt attcgttttc cctaatcact tttaaaattt
    25561 taccacttag tgtagtcata aacgctttaa gtttcattga ttatttttat aaaatatcaa
    25621 tggtacaact tatcagaatg acttacgctt aaatcaccta ttatattttg agtaaactga
    25681 atttacactc atgacgatta tctataggcg tt
//
LOCUS       DAKTGS010000024        23896 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.024, whole genome shotgun sequence.
ACCESSION   DAKTGS010000024 DAKTGS010000000
VERSION     DAKTGS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.024"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            161..448
                     /locus_tag="O0286_000845"
     CDS             161..448
                     /locus_tag="O0286_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169232.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEV
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            454..1935
                     /locus_tag="O0286_000846"
     CDS             454..1935
                     /locus_tag="O0286_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0169233.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(2024..2380)
                     /locus_tag="O0286_000847"
     CDS             complement(2024..2380)
                     /locus_tag="O0286_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0169234.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            2857..3816
                     /locus_tag="O0286_000848"
     CDS             2857..3816
                     /locus_tag="O0286_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0169235.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(3862..3993)
                     /locus_tag="O0286_000849"
     CDS             complement(3862..3993)
                     /locus_tag="O0286_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169236.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(4057..4272)
                     /locus_tag="O0286_000850"
     CDS             complement(4057..4272)
                     /locus_tag="O0286_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0169237.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            4437..4988
                     /locus_tag="O0286_000851"
     CDS             4437..4988
                     /locus_tag="O0286_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0169238.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(5040..5978)
                     /locus_tag="O0286_000852"
     CDS             complement(5040..5978)
                     /locus_tag="O0286_000852"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCY0169239.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            6160..7521
                     /locus_tag="O0286_000853"
     CDS             6160..7521
                     /locus_tag="O0286_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCY0169240.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            7508..9181
                     /gene="menD"
                     /locus_tag="O0286_000854"
     CDS             7508..9181
                     /gene="menD"
                     /locus_tag="O0286_000854"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCY0169241.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            9168..9971
                     /gene="menH"
                     /locus_tag="O0286_000855"
     CDS             9168..9971
                     /gene="menH"
                     /locus_tag="O0286_000855"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCY0169242.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            9964..10785
                     /gene="menB"
                     /locus_tag="O0286_000856"
     CDS             9964..10785
                     /gene="menB"
                     /locus_tag="O0286_000856"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCY0169243.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(11024..11353)
                     /gene="sspC"
                     /locus_tag="O0286_000857"
     CDS             complement(11024..11353)
                     /gene="sspC"
                     /locus_tag="O0286_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCY0169244.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(11391..12572)
                     /gene="sspB"
                     /locus_tag="O0286_000858"
     CDS             complement(11391..12572)
                     /gene="sspB"
                     /locus_tag="O0286_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCY0169245.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(12654..13664)
                     /gene="sspA"
                     /locus_tag="O0286_000859"
     CDS             complement(12654..13664)
                     /gene="sspA"
                     /locus_tag="O0286_000859"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCY0169246.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
     gene            complement(14192..15346)
                     /locus_tag="O0286_000860"
     CDS             complement(14192..15346)
                     /locus_tag="O0286_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0169247.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(15542..16552)
                     /locus_tag="O0286_000861"
     CDS             complement(15542..16552)
                     /locus_tag="O0286_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCY0169248.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            16705..17124
                     /locus_tag="O0286_000862"
     CDS             16705..17124
                     /locus_tag="O0286_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0169249.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            17151..17261
                     /locus_tag="O0286_000863"
     CDS             17151..17261
                     /locus_tag="O0286_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169250.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(17331..21101)
                     /locus_tag="O0286_000864"
     CDS             complement(17331..21101)
                     /locus_tag="O0286_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCY0169251.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(21329..21763)
                     /locus_tag="O0286_000865"
     CDS             complement(21329..21763)
                     /locus_tag="O0286_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0169252.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(21918..22388)
                     /locus_tag="O0286_000866"
     CDS             complement(21918..22388)
                     /locus_tag="O0286_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCY0169253.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(22436..23653)
                     /locus_tag="O0286_000867"
     CDS             complement(22436..23653)
                     /locus_tag="O0286_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCY0169254.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT         8144 a         3436 c         4100 g         8216 t
ORIGIN      
        1 ttagataaat tagattattt aaaattatgc gtgatattat tatttttatc atgcaaataa
       61 ttataataac aatatttaat tattgtattt acagttgatt aatagcgttg tgaagtataa
      121 aattattttg taaaacaatt ttgttattaa gaggtgcgct atggaaaagg ggattttcaa
      181 ttacgataat gcgaatgtat taaaattaga tactaatcag ttaaatgaaa acataaaagt
      241 cattgacgat atatttaaaa attatgagca gatagagcca actatagaag tcgaaaatgg
      301 aaatacaaaa ttaaaattaa atggatattt tattgcgtca attataagcc cattaaattt
      361 aaataaactt aataatttat atgttgaaga agaattttat catacatata acgaattaat
      421 tgttaaatat actgaggtaa aggaataatt ataatgaaaa aaatatttat gatggtacat
      481 gagttagatg tgaataaagg tggtatgacc tcttcgatgt tcaatagaag taaagagttt
      541 tatgatgcgg acatacctgc tgatattgtt actttcgatt acaaaggaaa ctatgatgaa
      601 attattaaag ctttgaaaaa acaaggtaaa atggatcgaa gaacgaaaat gtataatgta
      661 tttgagtatt ttaaacaaat ttcaaataat aaacatttta agtctaataa attgttatat
      721 aaacatattt cagaaagact aaaaaatacg attgaaattg aagagagtaa aggtatttca
      781 agatattttg atataacgac tggtacatat attgcctaca ttagaaaaag taaatctgaa
      841 aaagtgattg atttctttaa agataataaa cgaattgaac ggtttagttt tatagataat
      901 aaagtgcata tgaaggaaac atttaatgta gataataaag tttgttatca agtattttat
      961 gatgaaaagg gatacccata tatttcaagg aatattaatg ctaataatgg tgctgtaggt
     1021 aaaacttatg tgttagttaa taaaaaagaa tttaaaaaca atttagcact gtgtgtttac
     1081 tatttagaaa aactaataaa agattctaaa gatagtatta tgatttgtga tggaccaggg
     1141 agttttccaa aaatgtttaa tacaaatcat aaaaatgctc agaaatatgg cgttattcat
     1201 gttaatcatc atgaaaattt cgatgatacg ggtgcattta aaaaaagtga gaaatatatt
     1261 attgagaatg cgaataaaat taacggtgta attgtattaa cagaggcaca aagattagat
     1321 attcttaatc aatttgatgt agaaaatatt ttcactatta gcaattttgt taagatacat
     1381 aatgctccaa aacattttca aactgaaaaa atcgtaggtc atatttctag aatggtacca
     1441 acgaagcgaa ttgatttgct tattgaagtg gctgagttag tcgtaaaaaa agataatgct
     1501 gttaaatttc atatatatgg agaaggatct gtcaaagata aaatagctaa aatgattgaa
     1561 gataaaaatt tagaaagaaa tgtttttctt aaaggatata caacaactcc acaaaaatgc
     1621 ttggaagatt ttaaattagt cgtttctaca tctcaatatg aaggtcaagg gttaagtatg
     1681 atagaagcaa tgatttctaa aaggcctgtt gttgcctttg acatcaaata cggaccaagt
     1741 gattttatag aagataataa aaatggttat ttaatagaaa accataatat taacgacatg
     1801 gctgataaaa tacttcagct tgttaataat gatgtattag cagcggagtt tggttcgaaa
     1861 gcgagagaaa acattataga aaaatattca acggaatcaa tattagaaaa atggttaaat
     1921 cttttcaata gctaaatgaa aaaatcctag gtattcatga gtacctagga tttttacgca
     1981 tgataaatac aaaaaaagag aggatagtcc tctcaaacaa tagttaataa aattgtagcc
     2041 atttcttcca gctgtaacta ctaatacaag ctaagttcat tacgatatgt atgatttcaa
     2101 attcataacc acttacatat cctcttgtaa gtttaagtgt gaagattgag cctaacataa
     2161 cacaaatata aaaaacatta acataaggcg ttaaaaagcc gataataagt gctaaacctc
     2221 caacaacttc accaattgac atcaatactg cgatggtagg tggtaaacct aaagagccta
     2281 agaagtgaat tgttccatct aaatcaacaa atttcaaagt accatgcact aagaatgatg
     2341 ttgctaacat ccatctaatt aaaagcatac ctaatttcat catgtcacct ccaacattca
     2401 tatttttaaa acatagtcca aattatattt gtaaacatgt ggaaagtaaa gattttttat
     2461 aagattaatt attaaatatt ctattgtatt gaacaattgt tgtagaaaga tgtttaagtc
     2521 atcagttact ttttgtattt ataaataaag attagtacag catagtgcaa agattatagg
     2581 gaaatgaatt aactattgca tcgctattta tgaatcgtaa acactattat gaaattgcga
     2641 gagtagtatg gacaattaga atgatggttt gagataaatg agtgttatta accagtgcgt
     2701 cattttcaaa attagatgcc atttgctgtt tataataaga agatttgata attaactatg
     2761 ataagaaaaa ataataatcc cctaaataca ttataaacaa attaaatgat aaaataataa
     2821 tatatatttc tactaaagtt gtttggaggt aaatgtgtga ataggaatat cgttaaacta
     2881 gttgtgttca tgctaatttt agttgtagca gtagcgggtt gtggtcaaaa agatactgaa
     2941 gagaaaactg aaatgacgac aataaaagat gaattaggaa ctgaaaaaat taagaaaaat
     3001 cctaaacgtg ttgttgtatt agaatatagt tttgctgatt atttagcagc attagatatg
     3061 aaacctgttg gtattgcaga tgatggcagc actaaaaata taacaaagtc agtaagagat
     3121 aagattgggg catatgaatc ggttggatct agaccgcaac cgaatatgga agtgataagt
     3181 aaattaaaac cggatttgat cattgcagat gttagcagac ataagaaaat caaatcagaa
     3241 ttgagcaaaa ttgctccgac aatcatgtta gttagcggta cgggagatta taatgcaaat
     3301 attgaagcat ttaaaacagt cgctaaagca gtaggcaaag agaaagaagg cgagaagcgt
     3361 ctggaaaagc atgataaaat attagcggag attagaaaga aaattgaaca gagtacgtta
     3421 aaatctgcat ttgcattcgg tatctcaaga gcaggtatgt ttattaataa tgaagataca
     3481 tttatgggac aattcttaat taaaatgggt attcaacctg aagtcacaaa agacaaaact
     3541 acgcatgttg gtgaacgcaa gggtggtcct tatatatatt taaataatga agaacttgcc
     3601 aatatcaatc caaaagttat gattttagcc actgacggaa aaacggacaa aaatagaacg
     3661 aaattcattg atcctgcagt ttggaaatca ttaaaagctg tgaaagataa caaagtttat
     3721 gacgttgacc gaaataagtg gttgaaatca agggggatta tcgcaagtga aagtatggca
     3781 gaagatttag aaaaaattgc agaaaaagca aaataaaaat acagcgctac tcgtaaatca
     3841 tataagagtg gcgctgtatt tttaattatg tttattatct gtcggatgtg atgatttacc
     3901 tgaaagttta tttcgaataa atttaattac ataaccgaca aggattgttt taacagttct
     3961 tttaatgaat tggcgcatcg ttacatacct catttctcta tatcttacga actatatacc
     4021 cattcatata tgctttttaa acgtcattgt cacaatttaa tttttaggga atataatata
     4081 accatcttta tctgcttttt tagtaaaaat gacaaaaatt gcatgtatta ttgagatgat
     4141 ggtagggata cctgtccaga aaaataataa gtgaaaaaga ccttgtccaa atttatcagc
     4201 ataaaattta tgaataccta aacctccaag aaataatgca acaataacat aaatggcttt
     4261 attgactttc atttgtaaat cctccttaac tataattcta cttaaattcg ttgtgaaaac
     4321 caatatttct aactttagaa ttttcaaact ttctaaaatt ataagtatat ctttttaaaa
     4381 taagctagaa tttctatata ataaatgtta ataacgtaaa agggaatgat gacatagtga
     4441 tacgtcaagc acgtccagag gaccgatttg atattgcgaa gttagtttat atggtttggg
     4501 atgatatgga attagaattg gtaaagcatc tacctaaaga catggtatta gatgcaattg
     4561 aaaaaagctg tgttgatgca acatatcgaa ctttttatca gcatatttta gtttatgaag
     4621 tagaaaataa agtagcaggt tgtattatta gctatagtgg tgaaaatgaa ttgaaatacg
     4681 aaaaagcatg ggaactactt gacttgccag aaaaaataaa acaatatggc acgccattac
     4741 ctgtaaaaga agctaaagac gatgagtatt atatagaaac aattgcgaca tttgcagcat
     4801 atagaggtag aggcatcgcg acaaagttat taacgtcatt acttgaatca aatacacatg
     4861 ttaaatggag tttgaattgc gatattaata atgaagcagc attaaagtta tataaaaaag
     4921 taggctttat atctgatgga cagattgaat tatacaagca catgtatcat catttaattg
     4981 ttaaataaaa aaactcgaca gttcgatgta agtcgattgc cgagtagtat cattttctat
     5041 taaatgcctg caaataatgc actaatataa atacctaatg catataataa accgaaaaat
     5101 gtatttgttt taccagcagc agccattgct ggcatcattg taggcggtgt atcattcttc
     5161 ttgaaacgtc tgataacttt aacaggcatt gggaatgata acaacgcaag taagtaaaat
     5221 aatgagccac caggtttaat aatgatcgta agtacaataa aggcataagc gataaagtac
     5281 atgattgcca taaatgttaa agaagcattt ttacctaata gaatgggtaa agttttgcga
     5341 ccacttgctt tatctttgac acggtcgcga atattgttag ccatattaat taaaccgata
     5401 gtgattacta taggtacact taaccaaatt acataacttt gaatattgcc agtttgaata
     5461 aagaatgcaa taacgataat aaacataccc ataaatacgc ctgagaataa ttcaccgaaa
     5521 ggcgtccatg aaatagggaa agggccacct gtatataggt aaccaacagc catacatact
     5581 aatccaactg gtaataacca aaatgaagag ttagcagcta aaaacaaacc taatattgct
     5641 gctaagatgt aaaatgcaat ggctaatcgt agcacaagct ctgggctcat accgttgcga
     5701 acaatggcac caccaatgcc tacagattca tgatcatcaa ggcctttttt ataatcatag
     5761 tattcattaa acatattagt tgctgcttga ataagtaagc atgctagtaa catggcaatg
     5821 aataggctga ttttaatatg atcttcgcta ccaagaaaat atattttaga tgctgctgta
     5881 ccaactaaaa cgggtactac ggaagcagtt aatgtatgag gacgcattaa atgccaatat
     5941 ttcttaactg tagaatattg ctgatattga ttactcattg aatacaaaac ctctttaaat
     6001 catatttttt ataacaaaga tattttaata gaaagtagca taaaggtcaa ttgacgtgaa
     6061 aaattgcaag ataagtttaa gcaattcttg taacacaatt atccaaaaat gatacaatat
     6121 aataatgagt gaaaaaagac gaagaaagaa gtgaaatgga tggctacggg cgtattagag
     6181 gacgatattg tcaaagagat atatggcagc tcaaaggaat gggtttcagt tgaagtgaaa
     6241 ttatcacagt cactagaccc gagcacatta tttcatctca ctgacaatga ggcaggagat
     6301 cgcttttata tgcgtttgaa tgataatcga acgtcatatt ttggctacaa agcaattcaa
     6361 ttattcaaaa ataattctaa aaataaacaa tctattttta aagactggga aaaattaaaa
     6421 cataacatca catttataca tccgcaatct gagaaacatc atcttcgagt tgttggaggg
     6481 ttccaatttt caagtcataa atcagatgat gaatggcgag agtttggact aaatcatttt
     6541 gtattacctg aagttttaat ttcaactgat aataatggga catttttaac ttatacagtt
     6601 aaaagggaaa gttttactgt tgaggcattg aacgatttaa tggatttgtt caacaatata
     6661 tcggacatag atgtggacga gcaaattggg ggaattacta gaaatgaaga tatttataaa
     6721 gatgactggc gtcaacttgt agtagaagct atagaatcta ttaataatga agaaaaaatt
     6781 gtactagcac gtagacggtt aataaagttc gataaagata tcagtattcc atatattcta
     6841 aagcaagcat attctaaaga aaaaaacagt tatatattct tgttagaatc acaagattct
     6901 atattctttt cacaaacacc tgaacaatta ataaaggtca ataataaaat actatcgact
     6961 aaagctgtag caggtacaat taaacgttca caagatgagg acgaagatac aaaaaatgtt
     7021 gaagcatttt taaaagataa taaaaactta atcgaacatc gatttgttgt tgacagtatt
     7081 ttacatgata ttaaacctta tatcactgaa ttacattatg ataagacgcc taaaattcta
     7141 aaaaatgatc atttatatca cttgtacact gaaataaagg cgccactgaa ggatgattcg
     7201 tatattagtt taattgataa tttacatcca acacctgctt taggtggcta tccaaaagaa
     7261 tttgcaatgg attttattga gcagaaagaa tttggtacgc gaggattata tggtgcaccg
     7321 gttggctata tagatatata cgatgattgt gaatttattg ttgcaattcg ttcgatgctt
     7381 attaagaaag cacaagcaac tttatttgct gggtgtggca ttgttaaaga ttctgatcca
     7441 gatagtgaat tggcagaaac gaaccttaag ttcacaccta tgatgaatgc attaggagtc
     7501 gatatgaatg ggaaatcata aagcagcttt aacgaagcaa gtttttacat ttgcatctga
     7561 gttatatgcg tacggcgtaa gggaagtagt tatcagtccg ggatcacgct caacgccact
     7621 tgcacttgca tttgaagcac atccaaatat taaaacatgg atacaccccg atgagcgaag
     7681 tgcagcgttt tttgcagttg ggttaattaa aggcagtgaa agacctgtcg ctatattatg
     7741 tacgtcaggt acagcagcag cgaattatac gcctgcaatt gctgaaagcc aaattagtag
     7801 aattccttta atcgttttaa caagtgaccg tccgcatgaa ttaagaagtg taggcgcacc
     7861 acaagcgatt aatcaagtaa atatgtttaa taattatgta agttatgagt tcgatatgcc
     7921 tattgcggat gatagtaaag agaccattga tgcaatttac tatcaaatgc aaattgctag
     7981 tcaatattta tatggaccac ataaagggcc aattcatttt aacttgccat ttagagatcc
     8041 gttaacacct gatttgaatg caacagaatt gttaacttct gagatgaaga ttttaccgca
     8101 ctatcaaaaa agtatagatg catcggcatt aagacacatt ttaaataaga aaaaaggttt
     8161 aattattgta ggggatatgc agcaccaaga agttgatcaa atactaacgt attcaacgat
     8221 atatgatttg cctattttag ctgatccttt aagtcattta agaaaatttg atcatccgaa
     8281 tgttatctgt acatatgatt tgctgtttag aagcggctta gacttaaatg tggatttcgt
     8341 aattcgtgtt gggaaaccag tgatttctaa aaagttaaat caatggttaa agaaaactga
     8401 tgcatttcaa atattagtgc aaaacaatga taagattgat gtctttccga tagcgccaga
     8461 tatttcatat gagatttctg cgaatgattt ctttaggtca ttaatggaag acacgacagt
     8521 taatcgcgta agttggttag aaaaatggca atgcttagag aaaaaagggc gtaaagaaat
     8581 taaatgttat ttggaacaag ctacagatga gagtgcattc gttggtgaat tgattaagaa
     8641 aacatctgaa aaagatgcat tatttattag taatagtatg cctatcagag atgtagataa
     8701 cttgttattg aataaaaata tagatgtcta tgcgaatcgt ggtgcgaatg gtattgatgg
     8761 tatcgtttca actgcactgg gtatggctgt gcataaacga ataacattat tgataggtga
     8821 tttatcattt tatcatgata tgaatggact attaatgtca aaattaaata atattcagat
     8881 gaatattgta ttattgaaca acgatggtgg cggtattttt tcatatttac cacaaaaaga
     8941 aagtgcaact gactattttg aacggttgtt tggcacaccg acgggattgg atttcgagta
     9001 tacagctaag ttatatcaat tcgattttaa acgttttaac agtgtttcag aatttaaaaa
     9061 tgccacattg ttatctgaaa cttcgacgat ttatgaattg ataacgaatc gcgaagataa
     9121 ctttaaacag catcaaattt tataccagaa attgagtgaa atgattcatg acacattata
     9181 aattttatga agcaaacgtt gagaccaatc aagttttagt atttctgcat ggatttctta
     9241 gcgacagccg tacttatcat aatcacatcg aaaaatttac tgataactat catgtcatca
     9301 ctatagactt accaggccat ggcgaagatc agtcttcaat ggatgaaacg tggaattttg
     9361 attatattac gacgttgtta gaccgaattt tagataaata taaagataaa tcaataacat
     9421 tgtttggata ttcaatgggt gggcgtgttg cattatatta tgcaattaat ggtcacatcc
     9481 ctatatctaa tttgatatta gaaagtacgt caccaggtat taaagaagaa gcaaatcaat
     9541 tggaacgccg tcttgttgat gatgcacgtg ctaaagtatt agacatagca ggtattgaat
     9601 tatttgttaa tgattgggaa aagttgccat tatttcaatc gcaactagaa ttaccagttg
     9661 aaatacaaca tcaaataaga caacaacgat tgtctcaatc gccacataaa atggccaaag
     9721 cattaagaga ttatggtaca ggtcaaatgc caaacttatg gccgcgcctg aaagaaatta
     9781 aagtaccaac attaatatta gctggagaat atgatgaaaa atttgtacag attgcgaaaa
     9841 aaatggcaaa tttaattcct aatagtaaat gtaaattaat ttctgctaca ggtcatacaa
     9901 ttcatgtgga agatagtgat gaatttgata caatgatatt aggattttta aaggaggagc
     9961 aaaatgacta acagacaatg ggaaacactt agagaatatg atgaaatcaa atatgaattt
    10021 tacgaaggga ttgctaaggt aacaataaat cgccctgaag tacgcaatgc gtttacacct
    10081 aaaacagttg ctgaaatgat tgacgcattt tcacgtgcac gtgatgatca aaacgtttca
    10141 gttatcgtat taactggtga aggtgattta gcattctgtt ctggtggtga ccagaagaaa
    10201 cgtggacatg gtggttatgt aggtgaagac caaatccctc gcttaaatgt attagattta
    10261 cagcgtttaa ttcgtattat tccaaaaccg gttatcgcga tggtaaaagg ttatgctgta
    10321 ggtggcggta atgtactaaa tgttgtttgt gacttaacga ttgctgctga taatgctatt
    10381 tttggacaaa ctggtcctaa agtaggttca tttgatgcgg gttatggttc aggatattta
    10441 gcacgtatcg ttggacataa gaaagcacgt gaaatttggt acttatgtcg tcaatacaat
    10501 gcacaagaag ctttagatat gggtctagta aatacagtgg tacctttaga gaaagttgaa
    10561 gatgaaactg tgcaatggtg taaagagatt atgaaacact caccaacagc gttacgattc
    10621 cttaaagcag ctatgaatgc tgacacagat ggtttagctg gtttacaaca aatggctggg
    10681 gatgcaacat tgctttatta cacaactgat gaagcgaaag aaggccgtga tgcgtttaaa
    10741 gaaaaacgtg atcctgactt cgatcaattc cctaaattcc cataagttat tttgaatatg
    10801 atattaagtc acttgcgtcg tttattagcg acgataggtg gcttttttat tttataagaa
    10861 tttaaaaggg cattttaatt aattatcata aagataagat agtttgagaa aaattaatag
    10921 agaaagaata aatgttaaag attattatta caatgaaaaa acgagctggt gagaaattga
    10981 attctcgcca acccgcttta tgaagtgaaa tcatttaaat tagttatact aagcgctcat
    11041 aaacgattgg tcgcgaagtg ccaatacctt gtttggatga cgtttgcatg aggatatgct
    11101 gtgtatcatc ataatcaaga atataaatga tttcttcatt atcaatagat gaaaatttaa
    11161 tgcgttgatg tgccgtatct ataaaaagaa tatgatattg attgtgactt gtatcatcgc
    11221 catgacgtat acaaattgat ttttgtaatt gatgattact ccaattacca atgaataaat
    11281 tgatattggt ttgttctaga tgtgtgagtt tggttgtgtc gtaaactaaa tttataaatt
    11341 gtagttgata catagtttta cctgccttta atcaatatct atattacttt ttagtaacct
    11401 atcattgaac cataccagtt ataatcacga ttgaatgata aatgtaatag gcttgaatct
    11461 gcatcttgga ttgataattc tgtatcccaa ggattccagt aaataagttt ttcttggtca
    11521 ttaattttag cattaccaac aactgctagc gcatgtccta aatgtgggtc attagggttt
    11581 tgagatacac tttgtgcaag gatcataatt cctacattat cttttgtaag ttgatcaact
    11641 tgttcatatg atggtacgcc ttcttgataa tgaatatctc tgccttgtga tttaccgtat
    11701 tcaatcattt gattagggaa tgttgcacaa ttaggaaggt cttgctcact tacttcaggg
    11761 tataatgtac gcataatatc atgtgcatta taagtgtctg tatttttagt tgcatttaat
    11821 aatgctgcca tactgaatcc tgcacaccat gagttatcga attgttgttc tctaattttg
    11881 aagtttttta atgtattttc atattgaact tgatcttctt gtatcgcttc gttttcttca
    11941 actttagtag gtgttactgt attttttaac tcttgtttca attttgctga aactgtttta
    12001 gcactttctt tttcttttac attaccaggt agaggcgtag cttttactaa tctaactttg
    12061 ccatcttctt caaaataaaa ccctttttca tcagtaagaa cagtgatatt tgaattttta
    12121 tctttaattt ggtctaaatc tttagcgatg aagtttgaaa ttttaacact gtaattcatg
    12181 tcttcttttg atttgtttaa atcatcttta tttttaggac taagtgttaa agtataaaca
    12241 attttaccgt cttttataac tggataatag taactattat cttcttcacc attaaattta
    12301 taaattttaa aagcttcgcc aagttcatat ttaccagttt ttgcattact ttgtttatct
    12361 aatgctttag cataaccagc aaattgttgt tgtgctagat cttttacttt ttgaggtact
    12421 ttgtcactct taacattaat ttctagctgt ttagagtgtg aatcggcttt ggctttattg
    12481 ttattagcaa atgcgcctag tgataaaata agcattgaaa ccattatgat gcttataata
    12541 ttgaatactc tagatttaca tgaactattc atataatacc ctccatttaa atcaaaatat
    12601 tttacgttgc taagactttg cttctatttt atctagacga acttaattgt taattaagct
    12661 gcatctggat tgtctgaatt attgttatcg ccattgtctg gattatcagg gttgttaggg
    12721 ttgtcagggt tatttggttc atctgggttg ttagggttat caggattgtt agggttatca
    12781 ggattatctg ggttattagg ttggtcatcg ttggcaaaat ggatatcttc aatattttgt
    12841 tttaagaagt tgcgtacatt ttcattaata aataccgcac cattaaattc atttggtaca
    12901 ccgccccaat gaattccgat cacttcattt ttttcattaa atacaggtga acctgaatta
    12961 ccaccagttg tacttaaatc atattgcata gcttcgcctt tgaggtaagt gatttttcct
    13021 ttactttccc acattgttgc tacaggttta tcaccaggat atcctgttac agtaatattt
    13081 tggttaactt gtgtttcagc attattactc attgttgctg gtttaactac ttcaccaata
    13141 tgtttgtttt gctcattagg ggagaattta actattgcta aatcaccttc gcctgaatat
    13201 ttagtgattt gttcagcagt gaaaccacca tttggataat tgtcttggtt aattgcagaa
    13261 gggaatgctt ttaaagcatg aggatcaccg tgcgtagcat ctacgacgtg tttatttgtt
    13321 aaaagagtat ctttacctac aactacaccg gaagcaataa atgtaccagt aggtgcttca
    13381 acttgaatat aagttacggg tgcataatga ccattcgttg tatctgtgat ttggtgacga
    13441 tcgttatttg gtaatataac atttgcgtgt tcacgttgtt ctaatggttt aaggttaccg
    13501 cctttttgaa tcttaggtgt ttgctgtttg cttgactgcg tttgttgtgg atgattgtcc
    13561 atagcctttg aagataacgc gtttgctgct ggagaactca caagtgtcgc tgttgtcaaa
    13621 gttgcaacga ataaagaact aacttttaaa aatttacctt tcatctaaaa acctccaaaa
    13681 aatttattta caagttaaat ataacactaa aaatttttaa gtcaataaga atatataaat
    13741 aaattaaaaa gtttttgtaa agattagagt ttacaaaaac attaagggaa ttaaaattga
    13801 attgtattat actttattca attgagttaa attgataaga tcgtcaattg tttgcattta
    13861 tctaaaagaa taaataagaa aggaattgag agcgaatgga aagtatttgt ttataataat
    13921 aaatagtaac ttttgaataa taattgtttg gccaaaggta gattaatatg aatggtgata
    13981 tattatttga gtgcttaagt ttcgaagaac aattataagt tagcatatta aaaccttttg
    14041 atagaatcat tttgagtgag gtgtttaaag gagaggaata taactcggta gtatttacag
    14101 tctctataga gtattattta aaacttatag aatataaaaa actgaacgta ctatgaattt
    14161 agttcacgac gtccagttca acatatcatt tttatttagt atttaaatat ttagcaagtc
    14221 tcttcatacc ttcttttaaa acatccattt cataagcata ggaaatcctt acaaatcctt
    14281 taccgaattc tgtaaaggat gagcctggaa cgattgctaa atgtgttgat tcaagtaaat
    14341 cgacacaaaa ttcgaaatcg tcatcggtga tatgtttaat acttgggaaa atataaaacg
    14401 cgccttcagg ttgagcggta atctcaaaac ctaatttagt taattcagat actaaataat
    14461 ttcttcgttc tacataagct tcgttcatat atttaggagc ttctaatcct tcgttaagtg
    14521 ctgttataca tgctatttga gctggaacat tggcacaaat acaattatag gcgtgcataa
    14581 atgttaattt atcaatcaaa tattgaggtc ctaatagaaa accaattctt attccggttg
    14641 ctgagtgtga tttacttaaa ccaccaatta atattaattg atcacgaatg tcttcaaatt
    14701 cagcgaagga tacatgttta ccactaaatg tattttcagc ataaatctca tcgctaataa
    14761 taaataacgg atattttttt aatacattta cgatatttaa aacttcattt ctttttaaaa
    14821 ctacgccagt tggattagtc ggataattta acaagacagc ttttgtcttt ggagaaatat
    14881 gactttctaa tgcatcaggt gtaattttaa attgtgttgc tgttgtatca atataaattg
    14941 gtttaccacc tagtacttcg atgagtggta tgtagcctgc ataaattggt cccggtatta
    15001 taatttcatc tccaggctct atgatagaac gtaacgttgt gtctattgct tcacttgctc
    15061 catttgtcac aataatttct tcaggatcat aggaaaaatg ataacgattt ttgaagtatt
    15121 gactaattgc ttcgcgagtt tctaataacc ctttattgtg agagtatgat gtcttgtcat
    15181 tgttaatagc atcaatatat gcctttttca caacatcagg catcgggaag tctggttggc
    15241 caatagttaa attaacacaa tcatccaaat tattcatacg atttgaaaat tggcgaatac
    15301 ttggtgctct taaatattta gaattagaat ttaaagaaag tttcatttgt gacacctcaa
    15361 atataaatca aatattgtct aaaaatttag aaattaataa tatcataaca tacttttaaa
    15421 gcaaaaaagg gtaaattact taagctttat ttgaaaaacg aaaaatgtct aaatacactg
    15481 tagtaactac ttttcaaaat gaataaagtg gttacagtta atgtacttag acagtataaa
    15541 attatgaatc tttaaactgt gatggtcttt gtaaattaat tattggattt gtccatttac
    15601 aaacaaagtt tgtagataat acatatacga tgattacaga tatacttatt aaataaagat
    15661 aagtcattaa tgatatagga ttatcgaatg ggtaccattc aaatcctcta acaatgccaa
    15721 taattaaacc atgtaataaa tatacgtata gcgtacgact accaatataa gtatataatt
    15781 ttttctttgt tgacattaaa tttagaaacg cagtcattgc gattaatata attccatata
    15841 atataagtcg tttaaaagga ctgaatatac tctgtccttc attttcaagt gaagtatatg
    15901 gtgaacttcc caataaccaa tctgcattga taggatgaat cacgtaaacg ataaaaaaca
    15961 aaataaaggt aatgatagat actggtatta gttttttatt tttaaaaata gccgtatgtt
    16021 ttttggtgaa aatgtaacct agataaaata ttgggaaaaa tacgattgtc cttgaaatgc
    16081 ttaagtagct atcgatgtta tctgaaaaac ctgctccaat agatataata attgaaactg
    16141 atagcacttt atatggatta aatcttctaa ctattactaa aatgacatga aagaaaaata
    16201 gcgtgatcaa aaaccataac gcaaatactg ggttaaaagg atcaagttgt aattcgtcac
    16261 ttttacctgt taagaaataa taaattgaaa agaatgcaaa aaatatcata taaggtacta
    16321 tcaaacgttt tgaaattttt tctaaatagt atggtttatc aatatttttc gcgaaataac
    16381 cagatataaa caaaaatgtt ggcatatgga aactataaat aactaggtat aatgctgata
    16441 aatatttatc gccactagtg tagggttgta acatatgtcc aaatacgact aataatatta
    16501 aaattgctct tgcgttgtca aaaaaataat ctctctcttt taatgaagtc atattttatc
    16561 tcctttatgt ccataagact cttactaatt tattatattt tgtattagtt atgcaacaaa
    16621 tttgtctatt gtgtcgaata gtattgtaaa aattataaaa aaagcatata atattataaa
    16681 taatatgtag tggagtagaa caacatgtat aaacaacttg aaaaacttat tacactgact
    16741 aacaatgact taaacttagt gaatagaaga tttggacaac gcacggatat caaatctgaa
    16801 cagctagaac ttctccgtat tttatttaat tacgatcgct tatcacagta tgatttgacg
    16861 atgaagatta gcagggaaca atctatagtt tcaaggtgga ttaagaaatt agttttgaaa
    16921 gggtacatca caagtcaaca atctagcgaa gatttaagat gtaaagaatt aattttgact
    16981 gatcaagcac gtacattaat ttcacaaata aataatgcac gttgcgaatt gattgaagca
    17041 agatgtcaat gtttatcgga agtcgaatta gacaatttaa atcaattact tgataagtta
    17101 aatcaacgac gcatatcgtt gtaacacgac aatagcaaac acataattta gtgtcgttgt
    17161 gcacacaaca aaaattgaat catgaattac aagcaaaagt agcggtgatt gttaaaattg
    17221 atggtaaaca atcaccgcta tttttgcttg tgtatgtata aaaaagggat caaaggtcat
    17281 cccccatgat tgatagtggg ggatgacttt tgatcctatg ttcatgttgc ttatttatat
    17341 tgtgggatgt cgaagtattt gccgacttcg ccaattttat catagtagcc tttgatgatt
    17401 ttagcattga tgttagccca atctacatct gtagcatatt ggtgtgttcc tggatgtgca
    17461 ggattccatc tcattttgta aagtgtattt tgaccagctt ttacatatga gttgccgatg
    17521 aatttagcac caccaacgat tgcttttgat actgtgtccc aaccagcttg tttagcatat
    17581 ttaatacctt cacgtaaagg atcgttatca tatgcagcaa taccaaatac gttatggtat
    17641 ttcgtgtttg agttagttac aactttgttg ttcactacat ctgcaccttt cgctaattga
    17701 gaagtaccgt tacctgtttc taatagggca tgtgagataa gataaacttc attaatgcca
    17761 tacatttgag cagctttgtt aaatgcagca ccttggtttt ctaatacacc tttacctttt
    17821 aagaattgat taattttatc aatagaaata ttttgtggtt ggtctaagcg taagaattga
    17881 tattttaatg ctggatcttg agctaaacgc ttcgtatcca ttgcatgctt aacatcatta
    17941 aagttagcat ctgtccactt acctggtaca cgttgtactt gtggtttata ttgtaaacca
    18001 gcttgtattt gagcaacttg gtttaatgtc atacctgttt gattatactt aattaattct
    18061 ttagctaaat cagttgattt aatccatgct aatttaccgt tagataattt accatagtac
    18121 caagtttgtc cattaatgac ttgttcttta acaactgcga atggttgttc attaaatgct
    18181 tttaatgagt atttagctgt atcagaattt ggtgttacat agtaataacc attaccattt
    18241 ttaattacat aagtgtagtt ataatctttg gcagctgatg tagttggttt cacagcggtt
    18301 ggtgcagtta aatcttttgc atttacccaa ccagtgcggt tattaatagt accgtataaa
    18361 taaacatctt tgcctacaga tacttgtttc gttgcattaa atgtaccttg agcaatgtta
    18421 ttgcctgtta aaatgacttg gtttttagta ccccaaggaa ccattgataa gccgttattt
    18481 gatttattaa cagtatattt ttgagtcgtt ttaacttctt tgcctaagtt ttgaacattt
    18541 aagtctttta cattgaacca acctaatggg atgttatggc ttgtattgtt taataataca
    18601 tacgtttcat taccatgagc acgctctttt gttacataga acgtacggtc tgcatatttc
    18661 gcaccgtttt tcgctgtttt ttcataaaca gaagcacgaa taccagtgtt gtttggttta
    18721 acttgagcaa tcttgctaac tgtttgagtc gtttgtggtt tagtaacagt ataagctttt
    18781 acagctgttt ttggttgtgc tactgctttt ttaggtgcag caggtacagc taaatatgct
    18841 ttacttaccc aaccagattt accatttaca gttccaaata aatagataga tttatcaatt
    18901 tgttgttgct tagtcgcttt aaaagtttgg ttacctgtac cagaaactgc accagcttct
    18961 tgtttataag tgccccaagg tactgaatat aatttagtgc ctggttttac tgtatatgtt
    19021 tgcattacat ttacaggtga ttttgcattg ttataaataa cgtcaccttg tttaacccaa
    19081 ccaattaaag ttggactatt gtaatcttta actaagtaga atttgtttcc acctaaactt
    19141 gcttcttttg ttacagcaaa tgttttttga acttctttcg ttggcttacc agttttgtca
    19201 taaactgtag tgaataagcc attgttttta gcattaattt gagcaacacc gtttaatgat
    19261 gaaactgtta atttattatt tgttgtaggt gttgatggct taggtgttgg tgtaggcgta
    19321 ggtttagcag tatcaactaa atatgcttta cttacccaac cagatttacc attcacagag
    19381 ccatataaat aaattgattt atcaatttgt tgttgctttg aagccttaaa tgtttggttt
    19441 ccagagccag acacactacc agcaacttgt ttagatgtac cccaaggtac tgtataaagt
    19501 ttcgtaccag gtttgattga atatgattga tttacattta caggtgattt agctgtgttg
    19561 taaaccacat cgccttcttt aacccaacca aatttattac cagaattgta atcttgaaca
    19621 agatagaatt tttgattacc taatgtagct gttttagata cagcaaatgt tttttgaact
    19681 tcattagttg ctttaccagt tttgtcgtat acagtagtat ataaaccact atttgttggt
    19741 ttgatttgtg cgacaccatt gtttgcagca actgttaatt taccagttga tggtttcgac
    19801 ggtgttgttg gttttgatgg tgtagtaggg gtagttgtag attgcgtacc ccatggcgcc
    19861 actttaccca tttttattaa atatttttca ttaattaagt catataattg atcataacta
    19921 taattatgac ttcttaaata tccatgtgga tcggcatggt cagtaccacc taaatattta
    19981 cttacagcgt agtgagtcca tactgtacca tttccatcat actcagcact gtctggtttt
    20041 aaaccataat attgtaattg tgtagctgca tagtcagcat agttattcat tgaacgtgca
    20101 aatgaagcat agtcgtgtgt gtgtacgatt tcaacattga tgaatctagg gttaccgact
    20161 gcaccgacac cccaagataa gtaatccgtt ggtgctgttt cgattatacg atccccatca
    20221 acaaatgcat gtacgaatgc gttttgatag ttatttttca tataactaat ttcaccattt
    20281 atcgtcgaac gatcattagc tgtatcatga actacgatac cttcaggacg acctacgccg
    20341 ttacggtatg cgtatttagg gaagtaagat gtataatctt cttcaatttt aggtgctttt
    20401 aagttatttt tacgaatgta atcgttaatt gaagagttta cttgtggttt atattttggc
    20461 aaactcgttt ttggtgttgc agcaactgat cttggttgtg ctgaagcgct aaaagtagtt
    20521 actttaggtg tcgcttcagt tttagcttta ggtgctgatg tagttgcagc tttaggtgct
    20581 gcggttttat attgcgtttc aagagctgca ggtttagcag ctgatttaat taattctgga
    20641 ttaatttgat tttctgaatt atcatcttca tcatcaacta aactataacc agcatttgta
    20701 acattagtgt tagttttagg tgctgtagtg cttgttgact ttgcaacagg ctgcgtatta
    20761 tttgtagtcg ctgattgatt agcacgagtg tcaccattta cttgtgcagt atcaactttt
    20821 tgacttactt gagcattgcc tgttttgtta tttgctgttt ttggttggac aatagcaggg
    20881 tcttgatata cttgagtgcc agaaatgttt tgcgttggat tttttacctc agcttttgct
    20941 tgttcagtag ttgctttaac tttattacta tctaaaacgt ttttattagt agtttgatct
    21001 tgtgtcgtct cagctgcttg aacttgatgt gcagtgactg ctgaacctac aagcgttaat
    21061 gcaaccattg atggtagttt gtaattgaat tttttcgcca ttctatttat tactcctaac
    21121 atttattaat tattactaac attatagtac ctgttttata tacctgtgcg tatttaacca
    21181 atttgtaata tactcttaat ttacattttc actgtaaaaa aatgaaaaaa attttgaatc
    21241 tcaatggtga cgtgacaaat caaatagaat tgatgtcaat ttaagaattg gtttattaca
    21301 aatgtttcta aaatagatac aacttttttt aattaagcga ggttaacttt tttgtcattt
    21361 caatatgctc gatgccttcc tcaagaaata tattacctct cattttaaag tttaaacttt
    21421 cataaaatgg gatagcatga cattgggcat tcatagtagc tacgtaaaaa ccttcatctt
    21481 tagctaatga ttctacagct tgcataagca ttctacccat tccttgtcca cgatgtgatt
    21541 tcatcacagc tactcgttct attttgacag ttgtttcatt aatagggcgt attcgagcag
    21601 tggcaactgg ctgtccatta tcatatccaa tgaggtgaat agattcagat tcatattcat
    21661 caatttcact ttcctcaggg acgccttgtt cttctacaaa cactttcttt cttatataga
    21721 agcaatcttc taacatcttt tgattgttta cttttgaaaa cataaaattc ctccacatcc
    21781 taacaaaatc atttacattt aatttactaa taataacata gaataacgat tgttattttc
    21841 ctcaaaaata attagacata aaaaagctat aaagatgatt gttgtgaatc atctttatag
    21901 ctaattaatt attaatgtta tagtgcttct tttctaataa ttgctgtata ttgccaaaag
    21961 attcgcattt gagcaatatt ccaattattc attcccattg taaatcctga aggcttaaat
    22021 atattcacat cagttacttc taaagctgct gcaactaaat attgtaatgg aatgctaagt
    22081 gatttcattg tttcggtaat gccattttca tcatataccc atgaaaatgg taactctgat
    22141 tcgaaaacat caacactatt gaaaatttca ggtagggcta aaatataaca tgcaccatct
    22201 acaataggat tgtcgatact atttttgtaa taaattagga gtgcttcata gttttctcga
    22261 tgctcatgat tgacgtaaaa aaattcactt ctaaaatgag ccatatcttg gctatgaatg
    22321 atttgttgtt ctaacatatt aaacatgcca tttatatttg caatcttttc gtatgttttt
    22381 ctactcatgt atgatgctcc tttcataaat agtaaatgtt aatcgtcata taatattaat
    22441 ttacaacacc attttggtta tttgaagctt gtggcgcttg ttgtgtgcca ccttgatttt
    22501 gatttgagtt ttgatctgta gcaggttgtt gttgattgct ggaatcactg ttattagttg
    22561 aatcactgtt ctcgttagat gtcttatctt tatctgtcgt atcattttgt tcttttctca
    22621 ataaactatt atctaaaggc gttaatggta ttaatgaacc ataatgatta atgacacgtt
    22681 gatctaagaa atcattttta tcattaatag gtgataattc taagtcttta cgaagtaagt
    22741 ttgcatattt ttgaatgctt tcaacacttg gatgataata gtaaatacca tttaacatat
    22801 catctttacc ttttaattgc gcagttttaa tttcaacatc atttgttaag tacatttttg
    22861 ctaaagcttt aatttcagag ttagttaaat tatgctttgc atttttacct acaatttgaa
    22921 tcacgttatc aagtttatca atagaatcaa cttcctgtgc tttttggaat aaaatcttaa
    22981 ttaattccat ttgacgttgt ccacgtttta agtctgaatc atgatgtcta gttctagcaa
    23041 ctgctaaagc ctcatcacca tttaattttt ggtacccttt tttaatttta atcttaccag
    23101 tatcatctgt gttaggttca tttaagtcgt atggcacatc atagtatatg ccaccaagct
    23161 catttacagc ctcgacaaat gctttcatat tgactctcac ataataatca acaggtacat
    23221 tcatggtagc ttctaccgaa tccattgcgg caattggacc accatatgca tgtgcatggg
    23281 taatcttatc gtaatagcca actttaggaa tgtagctgat agtatcacgt ggaatactaa
    23341 gcattctaat ttgatgtttt gattgattaa aagtagttaa aatcatagcg tctgatctag
    23401 agtgttcagc atcctgtcct ttttttcttc ttccatcgtt atcatcgata cctaagaaaa
    23461 gaatagagat aggttgttct tcgggattga ctttattatc tcttaagttg gattgacgat
    23521 tagcattttt gctgtcttga gaagattcga atgcatcttg ggacgtttta aaaagtaacg
    23581 tagcgaagac tattggaaca acaatgagaa ccaatgctag aaggatcaaa aagtatttta
    23641 aaaatttatt catgattgat gctcctatat taaatttttg ttaaatcata atcataattt
    23701 taaaatataa ttagttgaac gatatttata caacttcatt actaatacat tatagcctta
    23761 aattgtagta ctttatagta aaaaatacaa atgttatgtc aaagttcctt gtaatttgaa
    23821 tgttttgtta aaatcaaaaa gcatttttaa aaatataatg cgactttagt atgatgtctt
    23881 aaaaaattta aaggcg
//
LOCUS       DAKTGS010000025        23843 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.025, whole genome shotgun sequence.
ACCESSION   DAKTGS010000025 DAKTGS010000000
VERSION     DAKTGS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.025"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..302
                     /locus_tag="O0286_000868"
     CDS             <1..302
                     /locus_tag="O0286_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCY0169255.1"
                     /translation="LTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSDLM
                     IEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            292..1983
                     /locus_tag="O0286_000869"
     CDS             292..1983
                     /locus_tag="O0286_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0169256.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            1988..3226
                     /locus_tag="O0286_000870"
     CDS             1988..3226
                     /locus_tag="O0286_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0169257.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            3210..3983
                     /locus_tag="O0286_000871"
     CDS             3210..3983
                     /locus_tag="O0286_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0169258.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            3995..5158
                     /locus_tag="O0286_000872"
     CDS             3995..5158
                     /locus_tag="O0286_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0169259.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            5227..5505
                     /locus_tag="O0286_000873"
     CDS             5227..5505
                     /locus_tag="O0286_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCY0169260.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            5517..5849
                     /locus_tag="O0286_000874"
     CDS             5517..5849
                     /locus_tag="O0286_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169261.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            5846..6247
                     /locus_tag="O0286_000875"
     CDS             5846..6247
                     /locus_tag="O0286_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169262.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            6248..6643
                     /locus_tag="O0286_000876"
     CDS             6248..6643
                     /locus_tag="O0286_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCY0169263.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            6678..7319
                     /locus_tag="O0286_000877"
     CDS             6678..7319
                     /locus_tag="O0286_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0169264.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            7411..7866
                     /locus_tag="O0286_000878"
     CDS             7411..7866
                     /locus_tag="O0286_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCY0169265.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            7924..8274
                     /locus_tag="O0286_000879"
     CDS             7924..8274
                     /locus_tag="O0286_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169266.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            8316..8474
                     /locus_tag="O0286_000880"
     CDS             8316..8474
                     /locus_tag="O0286_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169267.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            8488..14688
                     /locus_tag="O0286_000881"
     CDS             8488..14688
                     /locus_tag="O0286_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0169268.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            14688..15512
                     /locus_tag="O0286_000882"
     CDS             14688..15512
                     /locus_tag="O0286_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0169269.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            15521..17104
                     /locus_tag="O0286_000883"
     CDS             15521..17104
                     /locus_tag="O0286_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0169270.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            17104..17394
                     /locus_tag="O0286_000884"
     CDS             17104..17394
                     /locus_tag="O0286_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169271.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            17410..19320
                     /locus_tag="O0286_000885"
     CDS             17410..19320
                     /locus_tag="O0286_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169272.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            19320..20786
                     /locus_tag="O0286_000886"
     CDS             19320..20786
                     /locus_tag="O0286_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCY0169273.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            20786..21175
                     /locus_tag="O0286_000887"
     CDS             20786..21175
                     /locus_tag="O0286_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCY0169274.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            21168..21332
                     /locus_tag="O0286_000888"
     CDS             21168..21332
                     /locus_tag="O0286_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCY0169275.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            21378..21677
                     /locus_tag="O0286_000889"
     CDS             21378..21677
                     /locus_tag="O0286_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0169276.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            21813..22115
                     /locus_tag="O0286_000890"
     CDS             21813..22115
                     /locus_tag="O0286_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0169277.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            22126..23580
                     /locus_tag="O0286_000891"
     CDS             22126..23580
                     /locus_tag="O0286_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0169278.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT         9116 a         3235 c         4813 g         6679 t
ORIGIN      
        1 aattaacaaa aaaacagctg aaagaatata tagaggatta taaaaaatct gatgacatat
       61 taattaattt gtatatagaa acgtatgaat tttattgtcg gttaagagat gaacttaaaa
      121 atagtgattt gatgatagag catacaaaca aggctggtgc gagcaatatt gttaagaatc
      181 cattaagcat agaactgaca aaaacagttc aaacactaaa taacttactc aagtctatgg
      241 gtttaactgc agcacaaaga aaaaagatag ttcaagaaga aggtggattc ggtgactatt
      301 aaagttttaa atgaaccttc accaaaacta ttaacaacat ggtatgcaga gcaagtcact
      361 caagggaaaa taaaaacaag caaatatgtt aaaaaagaat gtgagagaca ccttagatat
      421 ctagaaaatg gaggtaaatg ggtatttgat gaagaattag cgcaccgtcc tattcgattc
      481 atagaaaagt tttgtaaacc ttccaaagga tctaaacgtc aacttgtatt acaaccatgg
      541 caacatttta ttattggcag tttgtttggt tgggttcata aagaaacaaa actgcgcagg
      601 tttaaagaag ctttgatatt tatggggcga aaaaatggta aaacaactac tatatctggt
      661 gttgctaact atgctgtttc tcaagatgga gaaaacggcg ctgaaatcca tcttttagca
      721 aacgtaatga aacaagctag aatattattc gatgaatcta aggcgatgat aaaagctagc
      781 ccaaagcttg ataaaaattt cagaacatta agagatgaaa tccattatga cgcaacgata
      841 tcaaaaatta tgccccaagc atcagatagc gataagttag atggattgaa tacacacatg
      901 gggatttttg atgaaattca tgaatttaaa gactataaat tgatttcagt tataaaaaac
      961 tcaagagctg caaggttaca acctcttctc atctacatta cgacagcagg gtatcaatta
     1021 gatggtccac ttgttgatat ggtagaagcg ggaagagaca ccttagatca aatcatagaa
     1081 gacgaaagaa ctttttatta tttagcatct ttggatgatg acgatgatat taatgattcg
     1141 tcgaactgga taaaagcaaa tcccaactta ggtgtctcta taaatttaga tgagatgaaa
     1201 gaagagtggg aaaaagctaa gagaacacca gctgaacgtg gagattttat aaccaaaagg
     1261 tttaatatct ttgctaataa tgacgagatg agttttattg attacccaac actccaaaaa
     1321 aataatgaaa ttgtttcttt agaagagctg gaaggcagac catgcacgat tggttatgat
     1381 ttatcagaaa cagaggactt tacagccgcg tgtgctactt ttgcgttaga taatggtaaa
     1441 gttgcagttt tatcgcattc atggattcct aagcacaaag ttgaatattc taacgaaaaa
     1501 ataccctata gagaatggga agaagatggc ttattaacag tgcaagataa gccttatatt
     1561 gactaccaag atgttttaaa ttggataatt aagatgaatg agcattatgt agtagaaaaa
     1621 attacttatg atagagcgaa cgcattcaaa ctaaatcaag agttaaaaaa ttacgggttt
     1681 gaaacggaag aaacaagaca aggagctttg accttgagcc ctgcattgaa ggatttaaaa
     1741 gaaatgtttt tagatgggaa aataatattt aataataatc ctttaatgaa atggtatatc
     1801 aataatgttc agttgaaact agacagaaac ggaaactggt tgccgtctaa gcaaagcaga
     1861 tatcgtaaaa tagatggctt tgcagcattt ttaaacacat atacagatat tatgaataaa
     1921 gttgtttctg acaagggtga aggaaacata gaatttatta gtattaaaga tataatgcgt
     1981 taaggaggtg aatgttatcg caaaagagaa tattgtcaca cgcataaaga aaaaattgat
     2041 agacaattgg attgatcagt cagcttctaa gctttatgac tttagcccat ggaaaaataa
     2101 atctttttgg ggtgtaatca ataatacgct tgaaactaat gaaacgatat tttcagctat
     2161 tacaaagtta tctaattcga tggctagttt gcccttgaaa atgtatgaag attataaagt
     2221 agttaataca gaagtatctg atttacttac agtgtcaccg aataattctc tgagcagttt
     2281 tgattttatt aatcaaattg aaacaatcag aaatgaaaaa ggtaatgcat atgtgctaat
     2341 tgaacgagac atctatcatc aaccatcaaa gcttttctta ttaaatccag atgttgttga
     2401 aatgttaatt gaaaaccaat cacgtgaact ttattattcc attcatgctg caactggaaa
     2461 taaattgatt gttcataata tggacatgtt gcattttaaa cacatcgtgg catctaatat
     2521 ggtgcaaggc attagtccga ttgatgtgtt gaagaataca actgattttg ataatgcagt
     2581 aagaaccttt aatcttacag aaatgcaaaa acctgattct ttcatgctta aatatggttc
     2641 caatgtaggt aaagaaaaaa ggcagcaagt gttagaagat ttcaaacagt actatgaaga
     2701 aaacggtgga atattattcc aagagcctgg tgttgaaatc gaaccgttac ctaaaaaata
     2761 tgtctctgaa gatatagtgg caagcgagaa tttaacaaga gaaagagtag ctaacgtttt
     2821 tcaattgccc tcagtattct taaatgcaag atcaaataca aatttcgcga aaaatgaaga
     2881 gttaaacaga ttttacttgc agcatacctt attgccaatc gtcaaacagt atgaagaaga
     2941 atttaatcgg aaactactta ctaaaacaga cagagaaaaa aataggtatt ttaaatttaa
     3001 cgttaaatct tatttaaggg ctgatagtgc aacacaagca gaagtgtact ttaaagcagt
     3061 tcgtagtggt tactacacta taaatgacat tagagagtgg gaagatttac caccagttga
     3121 aggtggagat aagccgctaa taagcggtga tttataccca attgacacgc cacttgaatt
     3181 aagaaaatct ttgaaaggtg gtgataaaaa tgtcaatgaa agctaagtat tttcaaatga
     3241 aaagaaaatc aaaaagtaaa ggtgaaatat ttatttatgg tgatattgta agtgataaat
     3301 ggtttgaaag tgatgtaact gctacagatt tcaaaaataa actagatgaa ctaggagaca
     3361 tcagtgaaat agatgttcat ataaattcat ctggaggcag tgtatttgaa gggcatgcaa
     3421 tatacaatat gctaaaaatg catcctgcaa aaattaatat ctatgtcgat gccttagcgg
     3481 catcaattgc tagtgttatc gctatgagtg gtgacactat ttttatgcac aaaaatagtt
     3541 ttttaatgat tcataattca tgggttatga ctgtaggtaa tgcagaagaa ttaagaaaga
     3601 cagcggattt acttgaaaaa acagatgctg ttagtaattc agcttattta gataaagcaa
     3661 aagatttaga tcaagaacac ttaaaacaga tgttagatgc agaaacttgg cttactgcag
     3721 aagaagcctt gtctttcggc ttgatagatg aaattttagg agctaatgaa atagctgcta
     3781 gtatctctaa agagcaatat aagcgtttcg agaacgtccc agaagattta aagaaagatg
     3841 tagacaaaat cactaaaatt gatgatgtag atacatctga attggttgaa acacctaaag
     3901 aaagtatgtc actagaagaa aaagaaaaaa gagaaaaaat taaacgcgaa tgcgaaattt
     3961 taaaaatgac aatgaattat taggaggaaa tgaaatgccg acattatatg aattaaaaca
     4021 atccttaggt atgattggac aacaattaaa aaataaaaat gatgaattga gtcagaaagc
     4081 aacagatcca aatattgata tggaagacat caaacaacta gaaacagaaa aagcaggttt
     4141 acaacaaaga tttaacattg ttgaaagaca agtgcaagac attgaagaga aagaaaaagc
     4201 gaaagttaaa gataaaggag aagcttatca atctttaagt gataatgaga agatggttaa
     4261 agctaaggca gagttttatc gtcacgcgat tttaccaaat gaatttgaaa aaccttcaat
     4321 ggaggcacaa cgtttattac acgctttacc aacaggaaat gattcaggtg gagataagct
     4381 cttaccaaaa acactttcta aagaaattgt ttcagaacca tttgctaaaa accaattacg
     4441 tgaaaaagct cgtctaacta acattaaagg tttagagatt ccaagagttt catacacttt
     4501 agacgatgat gatttcatta cagacgtaga aacagcaaaa gaattaaaag caaaaggtga
     4561 tacagtcaag ttcactacta ataaattcaa agtatttgct gcaatttcag atactgtaat
     4621 tcatggatca gatgtagatt tagtaaactg ggttgaaaac gcactacaat caggattagc
     4681 agctaaagag cgtaaagatg ccttagcagt aagtcctaaa tctggattag aacacatgtc
     4741 attttataat ggatctgtta aagaagttga gggagcagac atgtatgatg ctattattaa
     4801 cgctttagca gatttacatg aagattatcg tgataacgca acaatttata tgcgatatgc
     4861 agattatgtc aaaattatta gtgttctttc aaatggaaca acaaatttct ttgacacacc
     4921 agcagaaaaa gtatttggca aaccagtagt atttacagat gcagcagtta aacctattgt
     4981 gggagatttc aattattttg gaattaacta tgatggaaca acttatgaca ctgataaaga
     5041 tgttaaaaaa ggcgaatatt tgtttgtatt aacagcatgg tatgatcagc aacgtacatt
     5101 agacagtgca ttcagaattg caaaagcaaa agaaaataca ggtccattac ccagctaagc
     5161 cccaaaaggt taatgtaaca gctaaggcta aatcagctgt aatatcagcc gaataggggt
     5221 gatgaaatga gtttagaaga aattaaattg tggttgagaa ttgactataa tttcgaaaat
     5281 gatttaattg aaggtctcat tcaatcggct aagtctgaat tactattaag tggggttcca
     5341 gattatgaca aagatgactt ggaatacccg cttttttgta cagcgattaa atatatcatt
     5401 gcaagagatt atgaaagtcg tggatactca aatgaccaat ctagaagcaa ggtgtttaat
     5461 gaaaaaggat tgcaaaaaat gattttgaaa ttaaaaaagt ggtaggtgat ttttaaatgg
     5521 aatttaatga atttaaagat cgcgcgtatt tttttcaata tataaacaaa ggaccatatc
     5581 cagatgaaga ggaaaaaatg aaattgtata gttgcttttg taaaatttat aatccttcta
     5641 tgaaagatag agaaatttta aaagcgactg aatcaaaatc aggattaacc ataattgtca
     5701 ggtcttctaa aactgaatat ctaccacaaa caaatcactt agttaaaatt gacagtgcat
     5761 tatattccga taaattattc aacattgtag aaataagaat tgatacacca gatattggct
     5821 ataatacagt ggttttatca gaaaaatgag tgtagaaatt aaagggatac ctgaagtgtt
     5881 gaataaatta gaatcggtat acggtaaaca agcaatgcag gctaagagtg ataaagcttt
     5941 aaatgaagca tctgaatttt ttataaaggc tttaaagaaa gagttcgaga gctttaaaga
     6001 tacgggtgcc agtatagaag aaatgactaa atctaagcct tatacaaaag ttggcagtca
     6061 agaaagggct gttttaattg aatgggtagg ccctatgaat cgcaaaaaca ttattcactt
     6121 gaatgaacat ggttatacaa gagatggaaa aaaatataca ccaagaggtt ttggagttat
     6181 tgcaaaaaca ttagctgcta gcgaacgtaa gtatagagaa attataaaaa aggagttggc
     6241 cagataaatg aatatattaa acaccataaa aggaatttta ttatctgatg cagagctcaa
     6301 aacacatata aattctagaa tatactatta caaagtcact gaaaacgctg aaacttccaa
     6361 accctttgtt gttattacac ctgtttatga tttgccttca gactttatgt ctgataaata
     6421 tctcagtgaa gaatacttaa ttcaaataga tgtagaatct tcaaataatc agaaaacaat
     6481 tgatataaca aaacgaataa gatacctgtt atatcaacaa aatttaattc aagcatctag
     6541 tcagttagat gcttattttg aagaaactaa acgttatgtg atgtcgagac gatatcaagg
     6601 catacccaaa aatatatatt ataaaaatca gcgcatcgaa taggtgtgct ttttaatttt
     6661 taaggaggaa ataagcaatg gcagaaggac aaggttctta taaagtaggt tttaaaagat
     6721 tatacgttgg agtttttaac ccagaagcaa caaaagtagt taaacgcatg acatgggaag
     6781 atgaaaaagg tggtacagtt gacctaaata tcacaggttt agcaccagat ttagtagata
     6841 tgtttgcatc taacaaacgt gtatggatga aaaaacaagg tactaatgaa gttaagtctg
     6901 acatgagtat tttcaatatt ccaagtgatg atttaaacac agttattgga cgtactaaag
     6961 ataaaaatgg tacatcttgg gtaggagaga atacaagagc accgtatgta acagtaattg
     7021 gcgaatcgga agatggttta acaggtcagc cggtatatgt agccttactt aaaggtactt
     7081 ttagtttaga ttcaattgaa tttaaaacac gaggtgaaaa agcagaagcc ccagaaccta
     7141 caaaattaac aggtgactgg atgaatagaa aagttgatgt tgatggaacg tcacaaggta
     7201 ttgtatacgg ttatcatgaa ggtaaagaag gagaagcaga attcttcaaa aaagtattcg
     7261 ttggatacac ggacagtgaa gatcattcag aggattctgc aggttcgtta cccagctaat
     7321 ccccaaaatg ttgaagtagc agttaattca aaatctgcaa cagtttcagc agaatagggg
     7381 ctttcaaaat aaatcaaagg agaataattt atgactaaaa ctttaaaggt ttataaagga
     7441 gacgacgtcg tagcttctga acaaggtgaa ggcaaagtgt cagtaacttt atctaattta
     7501 gaagcggata caacttatcc aaaaggtact taccaagtgg catgggaaga aaatggtaaa
     7561 gaatctagta aagttgatgt acctcaattc aaaaccaatc caattctagt ctcaggcgta
     7621 tcatttacac cagaaactaa atcaattatg gtaaataccg atgacaatgt tgagccaaac
     7681 attgcaccaa gcacagcaac gaataaaata ttgaaatata caagtgaaca tccagaattt
     7741 gttactgtag atgaaaatac aggagcaatt cacggtgtag ctgaaggtac ttcagtaatc
     7801 actgctatgt ctactgatgg aagcgataag tcaggacaaa tttcagtgac agtaacaaac
     7861 ggatagggat ttaaggcgca gtatatctgc gtctttttta tttgaataaa aggagctaat
     7921 acaatgatta aatttgaaat taaagatcgt aaaacaggaa aaacagagag ctatacaaaa
     7981 gaagatgtaa caatgggcga agcagaaaaa tgctatgagt atttagaatt agtaaatcaa
     8041 gagaataaaa aagaagcacc taacgcaaca aaaatgagac aaaaagagcg acagttatta
     8101 gtagatttat ttaaagatga aggattgact gaagaagatg ttctgaacaa gatgagtact
     8161 aaaacttata caaaagcctt acaagatata tttcgagaaa tcaatggtga agatgaagaa
     8221 gattcagaaa ctgaaccaga agagatggga aagacagaag aacaatctca ataaaagaca
     8281 ttttatcgaa cattaagaaa atacaacgtt tctgtatgga gcagtatggg tggacattaa
     8341 ctgaagtcag aaaacagccg tatgtaaaac ttttagaaat acttaatgaa gagaataaag
     8401 aagagactga agaaaaacaa agtgaacaaa aagtcattac aggtacggat ttaagaaaac
     8461 tttttggaag ctagaaagga ggttaatatg aatgaaaaag tagaaggcat gaccttggag
     8521 ctgaaattag accatttagg tgtccaagaa tgcatgaaag gtttaaagcg acaattaggt
     8581 gttgttaata gtgaaatgaa agctaatctg tcagcatttg ataagtctga aaaatcaatg
     8641 gaaaaatatc aggcgagaat taaggggtta aatgataggc ttaaagttca aaaaaagatg
     8701 tattctcaag tagaagatga gcttaaacaa gttaacgcta attaccaaaa agctaaatcc
     8761 agtgtaaaag atgttgagaa agcatattta aagttagtag aagccaataa aaaagaaaaa
     8821 ttagctcttg ataaatctaa agaagcctta aaatcatcga atacagaact taaaaaagct
     8881 gaaaatcaat ataaacgtac aaatcaacgt aaacaagatg cgtatcaaaa acttaaacag
     8941 ttgagagatg cagaacaaaa gcttaagaat agtaaccaag ctactactgc acaactaaaa
     9001 agagcaagtg acgcagtaca gaagcagtcc gctaagcata aagcacttgt tgaacaatat
     9061 aaacaagaag gcaatcaagt tcaaaaacta aaagtgcaaa atgacaatct ttcaaaatca
     9121 aatgataaaa ttgaaagttc ttacgctaaa actaatacta aattaaagca aacagaaaaa
     9181 gaatttaatg atttaaacaa tactattaag aatcatagcg ctaatgtcgc aaaagctgaa
     9241 acagctgtta ataaagaaaa agctgcttta aataatttgg agcgttcaat agataaagct
     9301 tcatccgaaa tgaagacttt taacaaagaa caaatgatag ctcaaagtca tttcggtaaa
     9361 cttgcaagtc aagcggatgt catgtcaaag aaatttagtt ctattggaga caaaatgact
     9421 tccctgggac gtacaatgac gatgggcgta tctacaccaa ttactttagg gttaggtgca
     9481 gcattaaaaa caagtgcaga ctttgaaggc caaatgtctc gagttggagc gattgcgcaa
     9541 gcaagcagta aagacttgaa aagcatgtct aatcaagcag ttgacttagg agctaaaacc
     9601 agtaaaagtg ctaacgaagt tgctaaaggt atggaagaat tggcagcttt aggctttaat
     9661 gccaaacaaa caatggaggc tatgccaggt gttatcagtg cagcagaagc aagtggtgca
     9721 gaaatggcta caactgcaac tgtaatggct tcagcgatta actctttcgg tttaaaagca
     9781 tctgatgcaa atcatgttgc tgatttactt gcgagatcag caaatgatag tgctgcagat
     9841 attcagtaca tgggagatgc attgaagtat gctggtactc ctgcaaaagc attaggagtt
     9901 tcaatagagg acacttccgc agcaattgaa gttttatcta actcaggttt agagggttct
     9961 caagcaggta ctgccctaag agcttcattt atcaggctag ctaatccaag taaaaataca
    10021 gctaaggaaa tgaaaaaatt aggtattcat ttgtctgatg ctaaaggtca atttgttggc
    10081 atgggtgaat tgattagaca gttccaagat aatatgaaag gcatgacgag agaacaaaaa
    10141 ctagctacag tggctacaat agttggtact gaagcagcaa gtggattttt agccttgatt
    10201 gaagcgggac cagataaaat taatagctat agtaaatcct taaagaattc caatggcgaa
    10261 agtaaaaaag cagcagattt gatgaaagat aatctcaaag gcgctctgga acaattaggt
    10321 ggcgcttttg aatcattagc aatcgaagtc ggtaaagatt taacgcctat gattagagca
    10381 ggagcggaag gtttaacaaa attagttgat ggatttacac atctccctgg ttgggttaga
    10441 aaagcttcag taggattagc actttttggt gcagcaattg gacctgcagt tcttgctgga
    10501 gggttattaa tacgtacagt tggaagtgct gctaaaggat atgcgtcatt aaatagacgt
    10561 attgctgaaa atacaatcct ttcaaatact aattcaaaag caatgaaatc tttaggtctt
    10621 caaacattat ttcttggttc tacaacagga aaaacgtcaa aaggctttaa agggttagcc
    10681 ggagctatga tgtttaattt aaaacctata aatgttttga aaaattctgc aaagctagca
    10741 attttaccgt tcaaactttt gaaaaacggt ttaggattag ctgcaaaatc tttatttgca
    10801 gtaagtggag gcgcaagatt tgcgggtgta gccttaaggt ttttaacagg acctataggt
    10861 gctacaataa ctgctattac aattgcgtat aaagttttta aaaccgcata tgatcgtgtg
    10921 gaatggttca gaaacggtat taacggttta ggagaaacta taaagttttt tggtggtaaa
    10981 attattggcg gcgctgttag aaagctagga gagtttaaaa actatcttgg aagtatcggc
    11041 aaaagcttca aagaaaagtt ttcaaaagat atgaaagatg gttataaatc attaagcgac
    11101 gatgaccttc tcaaagtagg agtcaacaag tttaaaggat ttatgcaaac catgggcaca
    11161 gcttctaaaa aagcgtctga tactgtaaaa gtgttaggga aaggtgtttc aaaagaaaca
    11221 gaaaaagctt tagaaaaata tgtgcattat tctgaagaaa atagcagaat catggaaaaa
    11281 gtacgtttaa actcgggtca gatatcagaa gacaaagcaa aaaaactttt gaaaattgaa
    11341 acggatttat ctaataacct tatagctgaa atagaaaaaa gaaataaaaa ggaactcgaa
    11401 aaaactcaag aacttattga taagtatagt gcattcgatg aacaagaaaa gcaaaacatt
    11461 ttaactcgaa ctaaagaaaa aaatgacttg cgaattaaaa aagagcaaga actcaatcag
    11521 aaaatcaaag aattgaaaga aaaagctttg agtgatggtc agatttcaga aaatgaaaga
    11581 aaagaaattg aaaagcttga aaatcaaaga cgtgatatca ctgttaaaga attgagtaag
    11641 actgaaaaag agcaagagcg tattttagta agaatgcaaa gaaacagaaa tgcttattca
    11701 atagacgaag cgagcaaagc aattaaagaa gcagaaaaag caagaaaagc aagaaaaaaa
    11761 gaagtggaca agcaatatga agatgatgtc attgctataa aaaataacgt caacctttct
    11821 aagtctgaaa aagataaatt gttagctatt gctgatcaaa gacataaaga tgaagtaaga
    11881 aaagcaaaat ctaaaaaaga tgctgtagta gatgttgtta aaaagcaaaa taaagatatt
    11941 gataaagaaa tggatttatc cagtggacgt gtatataaaa atactgaaaa gtggtggaat
    12001 ggccttaaaa gttggtggtc taactttaga gaagaccaaa agaaaaaaag cgataaatac
    12061 gctaaagaac aagaagaaac agctcgtaga aacagagaaa atataaagaa atggtttgga
    12121 aatgcttggg acggcgtaaa aagtaaaact ggcgaagcct ttagtaaaat gggcagaaat
    12181 gctaatcatt ttggcggcga aatgaaaaaa atgtggagcg gaatcaaagg gattccaagc
    12241 aaattaagtt caggttggag ctcagccaaa agttctgtag gataccacac taaggctata
    12301 gctaatagta ctggtaaatg gtttggaaaa gcttggcaat ctgttaaatc gacaacagga
    12361 agtatttaca atcaaactaa gcaaaagtat tcagatgctt cagataaagc ttgggcgcat
    12421 tcaaaatcta tttggagagg cacatcaaaa tggtttagca atgcatataa aagtgcaaag
    12481 ggctggctaa cggatatggc taataaatct cgcgcgaaat gggataatat ttctagtaca
    12541 gcttggtcga atgcaaaatc cgtttggaaa ggaacatcga aatggtttag taactcatac
    12601 aaatctttaa aagattggac tggggatatg tattcaagag cccacgatcg ttttgatgca
    12661 atttcaagtt cggcatggtc taacgctaaa tcagtattta atggttttag aaaatggcta
    12721 tccaaaacat atgattggat tagagatatt ggtaaagaca tgggaagagc tgcggctgat
    12781 ttaggtaaaa atgttgctaa taaagctatt ggcggtttga atagcatgat tggcggtatt
    12841 aataaaatat ctaaagccat tactgataaa aatctcatca agccaatacc tacattgtct
    12901 actggtactt tagcaggaaa gggtgtagct accgataatt cgggagcatt aacgcaaccg
    12961 acatttgctg tattaaatga tagaggttct ggaaacgccc caggtggtgg agttcaagaa
    13021 gtaattcaca gggctgacgg aacattccat gcaccccaag gacgagatgt ggttgttcca
    13081 ctaggagttg gggatagcgt aataaatgct aatgacactc tgaagttaca gcgtatgggt
    13141 gttttaccaa agtttcatgg aggtacgaaa aagaaagatt ggctagacca acttaaaggt
    13201 aatataggta aaaaagcagg agaatttgga gctacagcta aaaacacagc gcataatatc
    13261 aaaaaaggtg cagaagaaat ggttgaagca gcaggcgata aaatcaaaga tggtgcatct
    13321 tggttaggcg ataaaatcgg cgatgtgtgg gattacgtac aacatccagg gaaactagta
    13381 aataaagtaa tgtcaggttt aaatattaat tttggaggcg gagctaacgc tacagtaaaa
    13441 atagctaaag gcgcatactc attgctcaaa aagaaattag tagacaaagt aaaatcgtgg
    13501 tttgaagatt tcggtggtgg aggcgatgga agctatctat ttgaatatcc aatctggcaa
    13561 agatttggac gctacacagg tggacttaac tttaatggcg gtcgtcacta tggtatagac
    13621 tttggtatgc cttctggaac aaacgtttat gccgttaaag gtggtatagc agataaggta
    13681 tggactgatt acggtggcgg taattctata caaattaaga ctggtgctaa tgaatggaac
    13741 tggtatatgc atttatctaa gcaattagca agacaaggcc aacgtattaa agctggtcaa
    13801 ctgataggga aatcaggtgc tacaggtaat ttcgttagag gagcacactt acatttccaa
    13861 ttgatgcaag ggtcacatcc agggaatgat acagctaaag atccagaaaa atggttgaag
    13921 tcacttaaag gtagtggcgt tcgaagtggt tcaggtgtta ataaggctgc atctgcttgg
    13981 gcaggcgata tacgtcgtgc agcaaaacga atgggtgtta atgttacttc gggtgatgta
    14041 ggaaatatta ttagcttgat tcaacacgaa tcaggaggaa atgcaggtat aactcaatct
    14101 agtgcgctta gagacatcaa cgttttacag ggcaatccag caaaaggatt gcttcaatat
    14161 atcccacaaa catttagaca ttatgctgtt agaggtcaca acaatatata tagtggttac
    14221 gatcagttat tagcgttctt taacaacaga tattggcgct cacagtttaa cccaagaggt
    14281 ggttggtctc caagtggtcc aagaagatat gcgaatggtg gtttgattac aaagcatcaa
    14341 cttgctgaag tgggtgaagg agataaacag gagatggtta tccctttaac tagacgtaaa
    14401 cgagcaattc aattaactga acaggttatg cgcatcatcg gtatggatgg caagccaaat
    14461 aacatcactg taaataatga tacttcaaca gttgaaaaat tgttgaaaca aattgttatg
    14521 ttaagtgata aaggaaataa attaacagat gcattgattc aaactgtttc ttctcaggat
    14581 aataacttag gttctaatga tgcaattaga ggtttagaaa aaatattgtc aaaacaaagt
    14641 gggcatagag caaatgcaaa taattatatg ggaggtttga ctaattaatg caatcttttg
    14701 taaaaatcat agatggttac aaggaagaag taataacaga ttttaatcag cttatatttt
    14761 tagatgcaag ggctgaaagt ccaaacacca atgataacag tgtaactatt aacggagtag
    14821 atggtatttt accgggcgca attagttttg cgcctttttc attagtatta aggtttggct
    14881 atgatggtat agatgttata gatttaaatt tatttgagca ttggtttaga tctgtgttta
    14941 atcgcagaca tccttattat gttattactt ctcaaatgcc tggtgttaaa tatgcagtga
    15001 atacagctaa tgttacatct aatttaaaag atggttcttc aactgaaatt gaagtaagtt
    15061 taaatgttta taaagggtat tctgaatcag ttaattggac cgatagcgag ttcttattcg
    15121 actctaattg gatgtttgaa aatggaattc ctcttgattt cacacctaaa tatactcata
    15181 catcaaatca atttactatt tggaacggtt ctactgatac gataaatcca cgattcaagc
    15241 acgatttgaa aatattaatt aatttaaatg cgagtggagg atttgaactg gttaactata
    15301 caacaggtga tatttttaag tacaacaaaa gtatagataa aaacactgat tttgttttag
    15361 atggtgtgta tgcatatcga gatataaata gagtgggaat tgatacaaat agaggcatta
    15421 taacattagc gccaggtaaa aatgaattta agattaaagg agacgtcagt gatattaaaa
    15481 ctacatttaa gtttcctttt atttataggt aggtgattta atggattatc atgatcattt
    15541 atcagtaatg gattttaatg aattgatttg tgaaaattta ctagatgtag attatggttc
    15601 ttttaaagaa tattatgaac tgaatgaagc taggtacatc acctttacag tttatagaac
    15661 tactcataat agttttgttt ttgatttatt gatttgtgaa aacttcataa tttatcatgg
    15721 tgaaaaatat acaattaagc agacagcgcc aaaggttgaa ggtgataaag tttttattga
    15781 agttacggca tatcacataa tgtatgaatt tcaaaatcac tcagtggaat caaataagct
    15841 tgatgacgac agtagcgaaa ctggtaaaac gccagaatac tctttagatg agtacttaag
    15901 atatggattt gcaaatcaaa aaacttcggt caaaatgacc tataaaataa ttggagattt
    15961 taagcgaaaa gtaccgattg acgaattagg taacaaaaac ggcttagaat actgtaaaga
    16021 agcggtagac ctgtttggct gtataattta cccaaatgat acagagattg gtttttattc
    16081 tcctgaaaca ttttatcaaa gaagcgagaa agtgattcga tatcaatata atactgatac
    16141 tgtatctgca actgtcagta cattggaatt aagaacagct ataaaagttt ttggaaaaaa
    16201 gtatacagct gaggaaaaga aaaattataa tcctattaga acaactgaca ttaaatattc
    16261 aaatggtttt ataaaagaag gtacttatcg taccgaaaca attgggtcta aagctactat
    16321 taactttgat tgcaagtatg gtaatgaaac agttagattt acaataaaaa agggctctca
    16381 aggtggaata tataagttga ttttagacgg caagcaaatt aagcaaattt cttgttttgc
    16441 taagtcggtt cagtctgaaa caatagattt aataaaaaat attgataaag gcaagcacgt
    16501 tttagaaatg atatttttag gagaagaccc caaaaataga attgatatat cttcaaataa
    16561 aaaagctaag ccttgtatgt atgttggaac tgaaaaatca acagtcttaa atttaattgc
    16621 tgataattca ggtcgcaatc aatacaaagc aattgtcgac tacgtcgcag atagtgcaaa
    16681 gcagtttggg attcgatatg ctaatacgca aacaaatgaa gatatcgaaa cacaggataa
    16741 gctgttagaa tttgcaaaaa agcaaataaa tgatactcct aagactgaat tagatgttaa
    16801 ttatataggt tatgaaaaaa tagagccaag agatagcgta ttttttgttc atgaattaat
    16861 gggatataac actgaattaa aggttgttaa acttgatagg tcacatccat ttgtaaacgc
    16921 aatagatgaa gtgtctttca gcaatgaaat aaaagatatg gtacaaattc aacaagcact
    16981 taacagacga gttattgcac aagataatag atataactat caagcaaatc gtataaatca
    17041 tttatacact agtactttga attctccttt cgagacaatg gatataggga gtgtattaat
    17101 ataatggcaa cagaagaagt taaaatcaaa gcgctacttg aaaacgataa acagtacttt
    17161 ccagctacac actggaaagc tataaatggg ataccttatg caggcagtag tgatattgat
    17221 ggattgcctc aagacggtat catttcggta gatgataaaa ataaattaga taaattaaaa
    17281 ataggcgaag caggaattat tcaaaatagc attgtacaga aatccccaaa cggtaaattg
    17341 tggaaaataa cagttgacga tagtgggaaa cttggtacag tgctatttta ttagaaagga
    17401 aggtgcatta tggaaaattt gtatttaata aaggatttgg gagctttagc aggtcgagat
    17461 tatagagcta aagaaattca aaacctgcaa agaatagagc aatttgcgct tggcttgaca
    17521 acagagttta agttgcatca gaaagctaaa acaatgcaac acttcgctga gcaaatttat
    17581 tataatggta gatcgcaagc agcagtaaac aaatctttac aaagtcaaat taacgcactt
    17641 gttgtggcac cacgtaataa cagtgctaat gagattgttc aagctcgagt taatgtaaac
    17701 ggcgaaacct ttgacacatt aaaagaacat ttagacgatt gggaaaccaa aactcaaatt
    17761 aataaagagg aaactataag agaattaaat aagaccaaac aagaaattct tgatatcgag
    17821 tatcgttttg aacctgataa gcaagagttt ttatttgtga cagaacttgc acctcttaca
    17881 aatgcagtaa tgcaatcctt ctggtttgat aatagaacag gcatagtata catgacacaa
    17941 gctagaaata atggctatat gctaagtcgt ttaagaccta atggtcaatt tatagacagc
    18001 tcattgattg taggtggggg tcatggtaca cataacggtt atagatatat tgatgatgag
    18061 ttatggattt atagttttat cttaaatggt aataatgaga atacattagt tcgtttcaag
    18121 tatacgccta atgtggaaat tagctatggc aagtatggta tgcaagatgt atttacagga
    18181 cacccagaaa aaccctacat cacccctgtc ataaatgaaa aagaaaataa aattctatac
    18241 agaattgaga gacctagaag tcagtgggaa cttgaaaact caatgaatta tatagagata
    18301 agaagtttag acgatgttga taaaaatatt gataaagttt tgcataaaat cagtatccct
    18361 atgagactaa caaacgaaac ccaaccaatg cagggtgtga cttttgatga aaaatacttg
    18421 tattggtata caggagacag taatccaaat aatagaaact atttaacggc tttcgattta
    18481 gaaacaggag aagaagcgta tcaggttaat gctgactatg gtggaacact agattcattt
    18541 cctggcgaat ttgcggaagc agaaggtttg caaatatact atgacaaaga tagtggtaaa
    18601 aaagctttga tgctaggtgt tactgtcggt ggtgatggaa atagaacaca tcgtattttc
    18661 atgattgggc aaagaggtat tttagaaata cttcactcaa gaggcgttcc ttttatcatg
    18721 agtgacacag gtggtagagt taaaccttta ccaatgaggc ctgataaact taagaatctt
    18781 gggatgttaa cagagccagg tctttactat ttatacactg atcatacagt tcaaatcgat
    18841 gatttcccat taccaagaga atggcgtgat gcaggttggt tcttggaagt taagccacca
    18901 caaactggcg gtgatgtaat tcagatattg acgcgtaata gttatgcaag gaatatgatg
    18961 acttttgaaa gggtgctttc tggaagaact ggagacattt cggactggaa ttatgtgcct
    19021 aaaaatagtg gtaaatggga gagagtacct tcattcatca caaaaatgtc agatattaac
    19081 atagtaggca tgtcgtttta tttaactacg gatgatacaa aacgttttac agattttcca
    19141 actgaacgta aaggggtagc tggttggaac ttatatgtag aagcttcaaa cacaggtggc
    19201 tttgttcata ggctagttcg taatagtgtt acagcatctg ctgagatact attgaaaaat
    19261 tatgatagta aaacaagttc agggccatgg actttacacg aagggagaat tataagttaa
    19321 tgagtaattt agagaaatct gtagctataa atttagaaaa cacagcgcat tatgaaaata
    19381 tttcaaatct agatataact tttagaacag gagagagtga ttcttctgtt cttcttttta
    19441 atatcattaa aaataatcaa ccgttattac tgagtgaaga aaatatcaaa gcacgaatag
    19501 cgattcgagg taaaggagta atggtagttg ctccactaga aatattagat ccatttaaag
    19561 gtattttaaa atttcaatta cctaatgatg taattaaaag agatggaagt tatcaagctc
    19621 aagtttcggt tgcagaatta ggtaattcag acgtggtagt tgtagagaga actatcacat
    19681 ttaacgttga aaaaagtttg tttagcaaga ttccctctga aacaaaacta cactatattg
    19741 ttgagtttca agaattagaa aaaactatta tggatcgcgc gaaagcaatg gacgaggcta
    19801 taaaaaatgg tgaggattat gcgagtctga ttgaaaaagc taaagaaaaa ggtctatcag
    19861 atattcaaat agcaaaatct tcaagtattg atgaattaaa gcaacttgct aatagccgta
    19921 tatctgattt ggaaaataaa gcgcaagcat attcaagaac attcgatgag caaaagcgat
    19981 atatggatga gaaacatgaa gccttcaagc agtcagtgaa tagtggtggt ttagtcacaa
    20041 gtggttctac ttcaaattgg caaaaagcta agattactaa agatgatggt aagataatgc
    20101 agattactgg atttgatttt aataatccag aacaaagaat aggtgattca acccaattta
    20161 tttatgtttc gcaagctata aattatccaa gagatgttag tactaacggt actgtcgaat
    20221 atttagtagt aacttcagat tacaagcgta tgacttatcg accgaacggt acaaataaag
    20281 tgtttgttaa aagaaaagaa gcgggttcat ggtctgagtg gtcagaatta gctattaatg
    20341 attacaatac accttttgaa actgttcaaa gtgcccaatc aaaagctaat atggccgaaa
    20401 gtaacgctaa attatacgca gatgacaagt ttaataaaag gtattcggtt atttttgatg
    20461 gaacagcaaa tggtgtgggc tctacattgt acttaaatga gagtttagac caatttattt
    20521 tattaatttt ttatgggact tttccaggtg gtgactttac agagtttggc agtccttttg
    20581 gaggaggaaa gatttcattg aatccctcaa atcttccaga tggtgatgga aatggtggag
    20641 gtgtttatga gtttggatta actaaatcta gtcgtacatc tttaactata tcaaacgatg
    20701 tctatttcga cttaggaagt caaagaggct ctggtgcgaa cgcaaataga gggacaatta
    20761 acaaaattat aggagtgaga aaataatgca aatattagtt aacaagcgta atgagataat
    20821 ttcatacgct atcattggcg gctttgaaga aggtattgat attgaaaatt taccagaaaa
    20881 tttctctcaa gtttttagac ctaaagcctt taaatattca aatggggaaa tagtttttaa
    20941 cgaagattat tcagaagaaa aagatgactt gcatcaacag attgacagtg aagaacaaaa
    21001 cacagtcgct tctgatgaca tcttacgaaa aatggttgct agtatgcaga aacaagttgt
    21061 tcaaagtaca aagttatcga tgcaagttaa taagcaaaat gcactaatgg caaaacaact
    21121 tgtgacactt aataaaaaat tagaagaggt taaaggagag actgaaaatg cttaaattaa
    21181 tttcaccaac attcgaagat attaaaacat ggtatcaatt gaaagaatat agtaaagaag
    21241 atatagcgtg gtatgtagat atggaagtta tagataaaga ggaatatgca attattacag
    21301 gagaaaagta tccagaaaat ctagagtcat aggttataat cttatggctt tttaatttga
    21361 ataaagtggg tggtgtaatg tttggattta ccaaacgaca cgaacaagat tggcgtttaa
    21421 cgcgattaga agaaaatgat aagactatgt ttgaaaaatt cgacagaata gaagacagtc
    21481 tgagaacgca agaaaaaatt tatgacaagt tagatagaaa tttcgaagaa ctaaggcgtg
    21541 acaaagaaga agatgaaaaa aataaagaga aaaatgctaa aaatattaga gacatcaaga
    21601 tgtggattct aggattaata gggacgattc taagtacatt tgttatagcc ttgttaaaaa
    21661 ctatttttgg catttaaagg aggtgatcac catgcttaag ggaattttag gatatagctt
    21721 ttggtcgtgt ttctggtttg gtaagtgtaa gtaatattta agagtcagtg cttcggcact
    21781 ggctttttat tttggataaa aggagcaaac aaatggatgc aaaagtaata acaagataca
    21841 tcgtattgat cttagcatta gtaaatcaat tcttagcgaa taaaggtata agtccgatac
    21901 cagtagatga agaaagtgtt tcatcgatta tcttaacagt tgttgcttta tatactacat
    21961 ataaagataa tccaacatct caagaaggga aatgggcgaa tcaaaaatta aagaaatata
    22021 aagctgaaag taaatataga aaagcaacag ggcaagcgcc aattaaagaa gtaatgacac
    22081 ctacgaatat gaacgacaca aatgatttag ggtaggtgtt gaccaatgtt gataacaaaa
    22141 aaccaagcag aaaaatggtt tgataattca ttagggaagc agttcaatcc tgatttgttt
    22201 tatggatttc agtgttatga ttacgccaat atgttcttta tgttagcgac aggcgaaagg
    22261 ctgcaaggtt tatatgctta taatatcccg tttgataata aagcaaagat tgaaaaatat
    22321 ggtcaaataa ttaaaaacta tgacagcttt ttaccgcaaa agttggatat tgtcgttttc
    22381 ccgtcaaagt atggtggcgg agctggacac gttgaaattg ttgagagcgc aaatttaaac
    22441 actttcacat catttggtca aaactggaac ggtaaaggtt ggactaatgg cgttgcgcaa
    22501 cctggttggg gtcctgaaac tgtgacaaga catgttcatt attatgacaa tccaatgtat
    22561 tttattaggt taaacttccc taacaactta agcgttggca ataaagctaa aggtattatt
    22621 aagcaagcga ctacaaaaaa agaggcagta attaaaccta aaaaaattat gcttgtagcc
    22681 ggtcatggtt ataacgatcc tggagcagta ggaaacggaa caaacgaacg cgattttata
    22741 cgtaaatata taacgcctaa tatcgctaag tatttaagac atgcaggaca tgaagttgca
    22801 ttatacggtg gctcaagtca atcacaagat atgtatcaag atactgcata cggtgttaat
    22861 gtaggcaata aaaaagatta tggcttatat tgggttaaat cacaggggta tgacattgtt
    22921 ctagaaatac atttagacgc agcaggagaa agcgcaagtg gtgggcatgt tattatctca
    22981 agtcaattca atgcagatac tattgataaa agtatacaag atgttattaa aaataattta
    23041 ggacaaataa gaggtgtaac acctcgtaac gatttactaa atgttaatgt atcagcagaa
    23101 ataaatatca attatcgctt atctgaatta ggttttatta ctaataaaaa tgatatggat
    23161 tggattaaga aaaattacga cttgtactct aaactaatag ctggtgcgat tcatggtaag
    23221 ccaattggtg gagtggtagc tagtgaggtc aaagcgccag ttaaaaacga aaagaatccg
    23281 ccagtgccag caggttatac actcgataag aataatgtcc cttataaaaa agaacaaggc
    23341 aattacacag tagctaatgt taaaggtaat aatgtaagag acggttattc aactaattca
    23401 agaattacag gggtattacc caacaacaca acaattacgt atgacggtgc atattgtatt
    23461 aatggttata gatggattac ttatattgct aatagtggac aacgtcgcta tattgcgacc
    23521 ggagaggtag acatagcagg caaccgaata agcagttttg gtaagtttag tgcagtttga
    23581 taattgtata tgatgaatct taggcaggta cttcggtact tgcctattat ttaaaattaa
    23641 taaacagtta atttttacat gaatatatta aattttaaaa aaacaaacgt ttttagtata
    23701 taaattattt tgtgttcgta ttgtgtgcta tgattaaaaa gttgttatgg tcaactatat
    23761 cgtggtttta tgtttattat caatcaaaat ataaattatt tataatttgt ttggtaatga
    23821 acgggttttt ttcgaaataa tag
//
LOCUS       DAKTGS010000026        23465 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.026, whole genome shotgun sequence.
ACCESSION   DAKTGS010000026 DAKTGS010000000
VERSION     DAKTGS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.026"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..178
                     /locus_tag="O0286_000892"
     CDS             <1..178
                     /locus_tag="O0286_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169279.1"
                     /translation="ERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYSVF
                     AYGLHYFSNLKKNK"
     gene            395..1219
                     /locus_tag="O0286_000893"
     CDS             395..1219
                     /locus_tag="O0286_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0169280.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            1452..1988
                     /locus_tag="O0286_000894"
     CDS             1452..1988
                     /locus_tag="O0286_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0169281.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            2218..2949
                     /locus_tag="O0286_000895"
     CDS             2218..2949
                     /locus_tag="O0286_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCY0169282.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            3015..5420
                     /locus_tag="O0286_000896"
     CDS             3015..5420
                     /locus_tag="O0286_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0169283.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            5424..5738
                     /gene="nirD"
                     /locus_tag="O0286_000897"
     CDS             5424..5738
                     /gene="nirD"
                     /locus_tag="O0286_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCY0169284.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            5729..6706
                     /gene="cobA"
                     /locus_tag="O0286_000898"
     CDS             5729..6706
                     /gene="cobA"
                     /locus_tag="O0286_000898"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCY0169285.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            7015..10704
                     /locus_tag="O0286_000899"
     CDS             7015..10704
                     /locus_tag="O0286_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCY0169286.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            10694..12253
                     /gene="narH"
                     /locus_tag="O0286_000900"
     CDS             10694..12253
                     /gene="narH"
                     /locus_tag="O0286_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCY0169287.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            12246..12821
                     /gene="narJ"
                     /locus_tag="O0286_000901"
     CDS             12246..12821
                     /gene="narJ"
                     /locus_tag="O0286_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCY0169288.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            12814..13491
                     /gene="narI"
                     /locus_tag="O0286_000902"
     CDS             12814..13491
                     /gene="narI"
                     /locus_tag="O0286_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCY0169289.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            13511..13963
                     /gene="nreA"
                     /locus_tag="O0286_000903"
     CDS             13511..13963
                     /gene="nreA"
                     /locus_tag="O0286_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCY0169290.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            13987..15021
                     /gene="nreB"
                     /locus_tag="O0286_000904"
     CDS             13987..15021
                     /gene="nreB"
                     /locus_tag="O0286_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCY0169291.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            15044..15697
                     /gene="nreC"
                     /locus_tag="O0286_000905"
     CDS             15044..15697
                     /gene="nreC"
                     /locus_tag="O0286_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCY0169292.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(15955..16695)
                     /locus_tag="O0286_000906"
     CDS             complement(15955..16695)
                     /locus_tag="O0286_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0169293.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(16674..17588)
                     /locus_tag="O0286_000907"
                     /pseudo
     CDS             complement(16674..17588)
                     /locus_tag="O0286_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
     gene            17958..19127
                     /locus_tag="O0286_000908"
     CDS             17958..19127
                     /locus_tag="O0286_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCY0169294.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(19234..19662)
                     /locus_tag="O0286_000909"
     CDS             complement(19234..19662)
                     /locus_tag="O0286_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCY0169295.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(19878..20324)
                     /locus_tag="O0286_000910"
     CDS             complement(19878..20324)
                     /locus_tag="O0286_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0169296.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            20722..21087
                     /locus_tag="O0286_000911"
     CDS             20722..21087
                     /locus_tag="O0286_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCY0169297.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            21269..22546
                     /locus_tag="O0286_000912"
     CDS             21269..22546
                     /locus_tag="O0286_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCY0169298.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(22950..23306)
                     /locus_tag="O0286_000913"
     CDS             complement(22950..23306)
                     /locus_tag="O0286_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0169299.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT         8054 a         3389 c         4552 g         7470 t
ORIGIN      
        1 agaacgttat atcaaagttt taatattata tattttcaca acactactat cttctatttc
       61 agtaacgtca aaatgtgtac ctaataaagt tataaggttt attttacgta ctgcaattgg
      121 ttacagtgta tttgcatatg gtttacatta cttttcaaat ttaaaaaaga ataagtaata
      181 ctttacataa agcgagataa aatgagcgtc gtaatatctt aaatcataaa gatattagcc
      241 aatcacgcga tttatctcgc tgtttttatg aaagaaaact acattttcga gagaataaaa
      301 agatttttct gaaatttaat ctatcaatta cattgattta ctaatataat tagaaagtaa
      361 attttatttg atcaaagtaa atgggggaag tactgtgata gaaaataaga aaacagttga
      421 agatacatat tcaacaggcg caattgttga ttcaatatca tcttcagtac aaatgaagca
      481 agtaatggta cagcaaacac ctggccgata tatgcttaaa gcaatgatgg ccggattttt
      541 actctcaatc gttactgtat ttatgttcgg aataaagacg cagtttgcga gtactcacgt
      601 agatggactt atcaatttaa tgggagccat tgcgtttagt ttaggtttga ttttagttgt
      661 attaactaac tctgaattat taacaagtaa cttcatgtac tttacagtag gctggtatta
      721 taaagtcgtt tctgttaaga agatgacttg gattttatta tattgtttct taggaaatat
      781 tttaggtggg tttgtgttat ttttcttaat gaaatttgcg catgtcatga cacctgaaat
      841 gacacaagct ttaacagctt tagttcagaa aaagactgtt gattcaacat ggttgaatat
      901 ttttacaaaa ggtattttct gtaacttctt tattaatatt ggtattttca tctctatgca
      961 attcaaaggc ggcttaacaa aggcattctt catagcttgt ggtgtcgtgg tctttgtata
     1021 catgggatat gagcacgttg tgttcaatgc aggtctatat gcaggtatgg tattctttaa
     1081 ccttgatgca gtttcatggt tacacgtttt aaaaaatatc gtttttgctt tcttaggtaa
     1141 ctttgtcggt ggcggtattt ttgtaggtct cgtttatgct ttcttaaatg gtaagcgtaa
     1201 tcgtttagag caacaataat taagatgtag tgattaaata gcgtaacaat gaatcattct
     1261 tccaaaccat tcatgaattt gaatggtttt ttacgttgag agagtaattt ttaataagtt
     1321 tcatgagagt attggtgtat tcagatgaag catactttct gacatgtgat aaatttattg
     1381 atacactttg atggatattt attttaaaaa taaaatatgg ttaaatcatt taaaaaagag
     1441 tggggattaa tatgacaaaa acgatgattc gactcgcaag tgaacaagat gcagaaaaat
     1501 tgcaacaact catgcatgaa gcatttacgc ctctaagaga actaggtatt gattggcctt
     1561 cggttaatgc taatttagat gctgttaaag aaaatataga taaaaatact acatttgtca
     1621 tgacaattga tgatgaaatt atatctacga ttacggttag atatccttgg ggaagtgtga
     1681 aaagtatttc gggttatcct tttgtctggt ggtttgcgac aaatcctgag tatgaaggga
     1741 aaggctatgg tagccaatta cttacttatg ttgaagaagc atttttgcga gatacgttaa
     1801 agtcagcggc agttacttta ggtacatctg caagattgca tccgtggtta cttaaaattt
     1861 atgaaaaaag aggttatgaa atatacagcg aacatgaaaa tgatgatggc gatttaggtg
     1921 tgatcatgcg caaagtattg atacccgagc gctttgacga aacaatatta ggccaaccac
     1981 cattttaaga taataatata cactacaagc gaccgatata ttaatgcttg ctcaaataaa
     2041 tgttgagtag ggattcatat atcggtcgct ttttttgatt taacaaaaag ggaaaatgta
     2101 taaggaatat gcatgactac aggcatcagg gaattccctg attttacatt ataatgtgat
     2161 tcttttcaca aaaacaaaat gatttggtat acaatacgtg tgaaagggtt ggtaaatgtg
     2221 aatgggaata tcattgttgc acatggcatg aggcacggac gacagaatca agcgttagaa
     2281 gcatttattt ctgaattagt aaaagatgat atacatcact atgatattgc gtttttagaa
     2341 agtgagcatc aggatttaga gacggtaatg acgacgttga ttcaaagtgg ggttgaccat
     2401 tttaaaattg taccattgct aatctttagt gcaatgcatt atctcaagga tataccgaat
     2461 atcgttcatg aaatgaaacg acggtatcca gacataaagg tagaagtaag cgagccactt
     2521 ggcacacatc ctttaatgag acgattaatt gaacaacgta tttgtgacgc attagcaggt
     2581 gacgaacaac aagttggagt tatggttgtt gcacacggca atattaatgg aaagtttact
     2641 aaggcacacg aagagttaca aatatgcgct caaaatttgc aaattagcaa gccgacatat
     2701 gcgagaacgc tatatggaga gattagtttt acacatgatt tagaaagcat ctcgaaacgg
     2761 tatcaaaaat tgattgtcgt accattattt ttatatgacg gaagacttgt taacaaagtg
     2821 aagcagcaaa tgaatgacat ggttattaat acagatatac acttcacacc ttcaattaac
     2881 ttcgacccaa ttttaaagca aatcattaac gatagattag aaagcttaat gattccaatg
     2941 aaaatataaa agcactaacg aaaaattaat accttttttt gaaataaata ttatggagta
     3001 aaggggcata ttttatggca aagcaaaaac tagtaatgat tggtaacggt atggcgggca
     3061 ttcgaacaat cgaagaaata ttagagcgcg ccaacgattt atatgatatt acggtaatag
     3121 gtaaagaacc ttatccaaac tataaccgca tcatgctttc aaatatttta caaaataaaa
     3181 tgacagttga agaaacaatt atgaatccat atgagtggta tgaggaacat ggtatcgagt
     3241 taattacaaa tgatcctgta attgaagtag atagagcaaa tcaatcagtt actactgcaa
     3301 atggtattga agtatcatac gataaattga tttttgcgac aggttctaaa gcattcgtca
     3361 ttccagttcc aggttcgaca ttaccgagtg taattggttg gagaacgctt gatgatactg
     3421 aacaaatgat gaacattgct aagactaaaa agaaagcaat tgtaattggt ggaggattac
     3481 taggtttaga gtgtgcacgt ggtttattag atcaaggtat ggaagtaacg gtgttacatt
     3541 tagctgaatg gttgatggaa atgcaactag accgtaaagc tggaaatatg cttaaagcag
     3601 atctagaaaa gcaaggtatg aagtttgaaa tgcaagccaa cacaactgaa atcttaggag
     3661 aagatgatgt tgagggtgtt aaattggctg atggacgcga gattccggca gacttagttg
     3721 ttatggcagt aggtatacga ccatacacag aagtagccaa agaatcaggt ctagatgtta
     3781 atcgtggtat tgttgtcaat gatgtgatgc aaacaagtga tagcaatgta tatgcagttg
     3841 gtgaatgtgc agaacataac ggcaaagttt atggactcgt tgcaccacta tatgaacaag
     3901 gtaaagtatt agctgatcat ttaacaaata aagaaacgaa cggatacaag ggatcaacaa
     3961 catttacgtc attaaaagtt tctgggtgtg acttgtatag tgctggtcaa attgtagaaa
     4021 atgcagaaat taaaggtatt gaaatattta atagtgttga taataactat aaaaaaatct
     4081 ttttaaaaga cggtaatgta gttggtgcag tactatatgg tgatatcgat gatggttcac
     4141 gcttttataa catgatgaaa aaaggtgaat ccactgaaga ttacacactt gtatcattgc
     4201 ttactaaagg tggagaagag gcatcgctat caattgctga tatggctgat gatgaaacaa
     4261 tttgtggttg taatggtgtt gataaaggta ctatagtaaa tgcgattacg gaaaatggct
     4321 ttacaacagt tgaagaagta acggctaaaa caaaagcggg gaattcatgt ggtaaatgta
     4381 aaccgcaaat tgctcaaata ttgcagcaca ccttaggaga tgactttgtt gccgcaaaac
     4441 ctgctggtat atgtggttgt actgatttga cacgcgatca aatagtaacg caaataagag
     4501 cgaaaggttt aaaaacatct aaagaagttc gacatgtttt aaactttaaa aataaaggtg
     4561 gatgtccaaa atgtcgacca gcaatcaact attatttaaa catggtttat ccacatgatc
     4621 atgaagatga aagagaatca agatttgcta acgaacgtta ccatgcgaat attcaaaatg
     4681 atggtacatt ttctgttata cctcaaatgc gtgggggtgt tacagatgca gaccaactga
     4741 ttcgtctagg agaagtggct aagaaatatc atgtgccact agttaaagtg acaggttcac
     4801 aacgtgttgg tttgtatgga gttaaaaaag aagaattacc aaatatatgg gaagacctag
     4861 gtatgcgttc agcatcagct tatggtaaga aaacacgctc agttaaaagc tgtgttggta
     4921 aagagttttg tcgatttggt acgcaataca cgacacgact tggcattcgt ttagaaaaaa
     4981 catttgaata catcgataca cctcataaat tcaaaatggg tgtatcaggc tgcccaagaa
     5041 gttgtgttga atcaggtgtt aaagattttg gtattatctc agttgaaaat ggattccaaa
     5101 tctatatcgg tggtaatggt ggtacagaag ttgaaaaagc tgaattttta acaactgtag
     5161 aaacagaaga tgaagtaatc aaattatgtg gtgctttgat gcaatattat cgcgaaacag
     5221 gtatatatgc tgaaagaaca gcgccatggt taagaagact tggatttgaa aatgtaaaag
     5281 aagtcttact tgacccagaa agacaaaatg aactatttga acgcattatg gatgctaaaa
     5341 aagcagtcga agctgaacct tgggaagcaa taactagcaa tgcgcaagca cgtagaattt
     5401 ttgaagtgga gaaggtgtaa tacatggaaa caaaagaaaa aattaaagtg acaactatag
     5461 atgaattaac acccctaatt ggaaaaaagg ttattgtcaa aggcaaagag gtagggttgt
     5521 ttttaacaga aagtggcaaa attcatgcga ttcacaatat ctgtccacac aaacaaggac
     5581 cattgtctga agggacagtg agtggggaat atgtattttg cccgctccac gatcaaaaaa
     5641 ttgatttaaa tacaggtatt gttcaagaac ctgatgaagg ttgtgtagat gtttatgagg
     5701 tagaagttac agacgggaac gtatatatat gtctgtagag gaatatggca aggtttactt
     5761 gataggtgcg ggtccaggta atccgaatta tttgacgaaa aaggctgagc gtttaatacg
     5821 tgaagcggat gtcattctat atgatcgatt agtgaatccg ttaattttac aatatgcgaa
     5881 tttgactact gaaattatcg atgttggtaa aaagccgtat gcaaaacata ttcagcaaga
     5941 aaaaataaat gactgtatcg tggaagcagc gcgtcgatat aacaaggttg ttagacttaa
     6001 aggtggcgat ccagcgatat ttggtcgtgt gcaagaagaa gtcgacacac taaatgaaca
     6061 tcatattgcg tttgaaattg taccaggggt gacatcagcg agcgcagcag ttgctactat
     6121 gcagacaggt ttgacaatgc gtacagttgc taaaagtgtg acattttcta caggtcactt
     6181 taaagattca gaagaaaatg aagtggatgt caattcctta gtgaatggtg gcacgttagc
     6241 aatttatatg ggggtaaaac gtttagggaa aattatcaca cagatacaac aatacacaga
     6301 tattgattac ccgatagcga ttgtctttca agcatcgtgt tttaacgagt ttgttgttaa
     6361 ggggcgttta agtaatatta tttcaaaatt gcaacactat tcaattgagg cgaaaccagg
     6421 tatatgtatt ataggggaag ttgttgatta tactgaaaac actcctaaat catatgatcc
     6481 tatgaagcaa ttttatgtag taagtggttc taaacatgac gcccttatgc tctgtgaaca
     6541 tttatatgac gaaggttatg gctgtttgct aaacccaaat gatacatcga atggcacata
     6601 tcattcgtcg caatatgatt actatgatgc atttattaag cagcaagaaa atgtaacata
     6661 tatttcaacc gatcgtgcag atgctaatac agtgttatgt cactaattta taaaaaataa
     6721 atgaataagt aaggtttcaa ccgagagaat atattcgtgt tgaagcctta tttgtcgttg
     6781 tgccaaattt gaacgattta gattggcaat aagcatgacc atacatgatt gtgtattatt
     6841 tcaatgaaat cccgttattg atagggaaat tccttaagta attaggtgat tccctaatat
     6901 tttatgtttg tgaaaaaatg tacaatctaa ttaaagcaat agtcttgggc attttaaagt
     6961 atttaaaaac acgacatcta cacatcgtat ttttatacgt aggaggatat aaatatggga
     7021 aaatttggat tgaatttctt taagccaaca gaaaaattta atgggaattg gtcgatccta
     7081 gaaagtaaaa gtagagaatg ggaaaaaatg tacagagaac gttggagcca cgataaagaa
     7141 gtaagaacaa cacatggtgt taactgtaca ggctcatgtt cttggaaagt atttgtgaaa
     7201 aatggtgtga ttacctggga aaatcaacaa actgactatc caagttgtgg tccggatatg
     7261 cctgaatatg aaccgagagg atgtccacga ggtgcgtcat tctcttggta tgaatacagt
     7321 ccgcttcgaa tcaaatatcc atatattcgt ggaaaactct gggatttatg gactgaagca
     7381 ttagaagaaa acaatggtaa tcgcgttgct gcatgggcgt ctattgttga aaatgaagac
     7441 aaagccaaac aatataagca agcccgaggt atgggagggc acgtgcgttc aaattggaaa
     7501 gacgttacag agataatcgc agcacaatta ctgtatacaa taaaaaaata tggtccagat
     7561 cgaatcgcag gatttacacc tattccagcg atgtcaatga ttagttatgc agcaggtgct
     7621 cgattcatca atttgcttgg tggtgaaatg cttagttttt atgactggta tgcagattta
     7681 ccacctgcct ctccacaaat ttggggagag caaacagatg tgcctgaatc aagtgactgg
     7741 tataacgcat catacattat tatgtggggc tctaatgtac ctttaacacg tactccggat
     7801 gcacatttta tgactgaagt ccgctataaa ggtacaaaag tcatttcagt agcaccagat
     7861 tacgcagaaa atgtgaaatt tgcagataac tggctagcac cgaatcctgg ttcagatgct
     7921 gcaattgcac aagcaatgac gcatgttatt ttacaagaac attatgttaa tcaacctaat
     7981 gaacgcttta taaattacgc taaacaatat acagatatgc cgtttcttat catgctggat
     8041 gaagatgaaa atggatataa agcgggtcga tttttaagag cgagtgactt aggtcaaaca
     8101 acagagcaag gcgaatggaa gccagttatt catgatgcaa tcagcgatag tttagtagta
     8161 cctaatggca caatgggtca acgttgggaa gaaggtaaga agtggaactt aaaactagaa
     8221 acagaagatg gttctaaaat taaccctaca ttatcaatga cagaaggtgg atacgaatta
     8281 gaaacaattc aattcccata ctttgatagt gatggagatg ggatattcaa tcgtccaatt
     8341 ccaactcgac aagtcacttt agcaaatggt gacaaagtcc gtattgctac aatttttgac
     8401 ttaatggcaa gtcaatatgg cgtgcgtcgt tttgatcata aattagaatc aaaaggatac
     8461 gacgatgcag aatcaaaata tacacctgct tggcaagaag ccatttcagg cgtaaaacaa
     8521 agtgttgtca ttcaagtagc gaaagaattt gcgcaaaacg ctatcgatac tgaagggcgt
     8581 tcaatgatta tcatgggtgc gggtattaac cattggttta actcagatac gatttatcgt
     8641 tcaatcttaa acttagttat gttatgtggc tgtcaaggtg tgaacggtgg cggttgggct
     8701 cactatgtgg gacaagaaaa atgtcgtccg attgaaggat ggagtactgt cgcatttgcg
     8761 aaagactggc aaggaccacc acgtttgcaa aacggaacaa gttggttcta ttttgcaaca
     8821 gaccaatgga aatatgaaga gtcaaatgta gatcgtttaa aatctccatt agctaaaaca
     8881 gaggagttaa agcatcaaca tccagctgat tataatgttt tagcagctag acttggttgg
     8941 ttaccatcat atccacaatt taataaaaat agtttgttgt ttgcagaaga agctaaagat
     9001 gaaggtatag attcaaatga agcgattttg caacgtgcaa ttgatgaagt gaaatcaaaa
     9061 caaacacaat ttgcaataga agatcctgat ttgaaaaaga atcatccaaa atcattattt
     9121 atatggcgtt caaacttaat ttcaagttct gcaaaaggtc aagaatactt tatgaagcat
     9181 ttacttggca caaaatcagg gttattagct acaccaaatg aagatgaaaa gccagaagaa
     9241 attacgtggc gtgaagaaac aacaggtaag ttagatttag tggtctcttt agacttcaga
     9301 atgacggcaa caccgttata ttctgacatt gttttgccgg cagcgacttg gtatgaaaaa
     9361 catgatttat catcaacgga tatgcatcca tatgtacatc ctttcaatcc tgccattgat
     9421 ccgttatggg aatcgcgttc agactgggat atttataaaa cgttggcaaa agcattttca
     9481 gaaatggcaa aagactattt acctggaacg tttaaagatg ttgtgacaac tccacttagt
     9541 catgatacaa agcaagaaat ttcaacacca tacggcgtag tgaaagattg gtcgaagggt
     9601 gaaattgaag cggtacctgg acgtacaatg cctaactttg caattgtaga acgcgactac
     9661 actaaaattt acgacaaata tgtcacgctt ggtcctgtac ttgaaaaagg gaaagttgga
     9721 gcacatggtg taagtttcgg tgtcagtgaa caatatgaag aattaaaaag tatgttaggt
     9781 acgtggagtg atacaaatga tgattctgtg agagcgaatc gtccacgtat tgatacagca
     9841 cgtaatgtag ctgatgcaat actaagtatt tcatctgcta cgaatggtaa attatcacaa
     9901 aaatcatatg aagatctcga agaacaaact ggaatgccgt taaaagatat ttctagcgaa
     9961 cgcgctgctg agaaaatttc gtttttaaat ataacttcac aaccacgaga agtaataccg
    10021 acagcagtat tcccaggttc aaataaacaa ggtcgacgat attcaccatt tacaacgaat
    10081 atagaacgtc tagtaccttt tagaacatta acaggacgtc aaagttatta tgtggatcac
    10141 gaagttttcc aacaatttgg ggagagctta ccagtatata aaccgacatt gccgccaatg
    10201 gtatttggga atagagataa gaaaattaaa ggtggtacag atgctttggt actgcgttat
    10261 ttaacgcctc atggaaaatg gaatatacac tcaatgtatc aagataataa gcatatgttg
    10321 acactattta gaggtggtcc aacggtttgg atatcaaatg aagatgctga aaaacacgat
    10381 atccaagata atgattggct agaagtgtat aaccgtaatg gtgttgtaac ggcaagagca
    10441 gttatttcgc atcgtatgcc taaaggtaca atgtttatgt atcatgcaca agataaacat
    10501 attcaaacgc ctgggtcaga aattacagat acacgtggtg gttcacacaa cgcgccgact
    10561 agaatccatt tgaaaccaac acaactagtc ggaggatacg cacaaattag ttatcacttt
    10621 aattattatg gaccaattgg gaaccaaagg gatttatatg tagcagttag aaagatgaag
    10681 gaggttaatt ggcttgaaga ttaaagcgca agttgcgatg gtattaaatt tagataaatg
    10741 cataggatgc catacgtgta gtgtgacatg taaaaacact tggacaaatc gtccaggtgc
    10801 tgagtacatg tggttcaaca acgtagaaac gaagccaggt gtagggtatc cgaaacgttg
    10861 ggaagaccaa gaacactaca aaggtggttg ggtactaaat cgtaaaggga aacttgaatt
    10921 aaaatctgga agtagaattt caaaaattgc tttaggtaaa attttttata acccagatat
    10981 gccattaatt aaagattatt atgagccatg gaactataat tatgaacatt taacaactgc
    11041 gaaatcaggg aagcattcgc cagttgctag agcgtattca gaaattacgg gggataacat
    11101 tgaaattgaa tggggaccta actgggaaga tgacttagca ggtggtcatg ttacaggtcc
    11161 aaaagatcct aacatccaaa aaatagaaga agatattaaa ttccaatttg acgaaacttt
    11221 tatgatgtat ttaccacgtt tatgtgaaca ttgcttgaat ccaagttgcg ttgcatcgtg
    11281 tccttcaggt gctatgtata aacgtgatga agatggcatt gtattagttg accaagacgc
    11341 atgtcgtgga tggcgttact gtatgacagg ttgtccatat aagaaagttt acttcaattg
    11401 gaaaacgaac aaagctgaaa agtgtacttt ctgtttcccg agaatcgagg cgggtatgcc
    11461 tacggtatgc tctgaaactt gtacaggacg tatgcgttac ttaggtgttt tattatatga
    11521 tgcagatcga gttcatgaag cagcttcagc agtagacgag aaagatttat atgaaaaaca
    11581 attagatata ttcttaaacc catttgacga agaagtcatt gctcaagctg aaaaagatgg
    11641 tattggttac gattggattg aagctgcaca aaactctcca atttataagt tagcaattga
    11701 atataaactt gcatttccgt tacatcctga atttagaacg atgccgatgg tttggtattg
    11761 cccaccactt agcccaatta tgagctattt tgaaggtaaa aatacaactc aaaatccaga
    11821 tgcgattttc ccagccattg aagaaatgcg tttacctatt gaatacttag ctaatatttt
    11881 cactgcagga gatacagagc cagtaaaagg agccctacaa cgtatggcaa tgatgagaag
    11941 ttatatgaga tctcaagtta ctcaacagcc atttgacacg tctcgattag agcgattagg
    12001 tataaccgag cgccaaacta aagacatgta tcgtttatta ggactagcca aatatgaaga
    12061 tcgatttgtt attccaacat cacacaaaga aacctattta gatacgtatc acgcacaagg
    12121 tagtacagga tacaattacg gcggcgagca ttttggagat aactgtgaag gctgtggcgt
    12181 tgcagtaggt tcagggaaaa ctggtcaaga aatttataat gagaatttct atggagggat
    12241 tttccgtgat taatttcgat aattttaaaa aatatcaaga aagttttgga tatatggcac
    12301 aacagttatg ttttccagaa aaattaactt ttcatccaaa aacttttgaa gaaacaattt
    12361 cgaaagatca tccagcatat gaagatttag tagcatttag aaatgtaatg atgacatttt
    12421 cattatcaga aatcaaagcg atttatacag atacatttga ttttacgaaa aaagcaccat
    12481 tatatatgac gtataacaaa tttgatacgc aaaaagaacg tgggcaaatg ttagctaaat
    12541 taaaggtatt atatgaaatg tttggactag aaatggtaga taatgaactt tctgattatc
    12601 taccgttgat gcttcaattt ttgcaagtgg cagaatggcg aaatgatcca agagctgaag
    12661 agaatattca attaatcatt atgattattg aggatggaac atatgtgatg gcgaatgcac
    12721 ttggtgaaga taacaatcca tatgcctatg tgattcaagc acttagaaaa acattgaaat
    12781 tatgtttgac atcaccaaaa ggagtgaaac atcatgctta atcagttttt atgggttata
    12841 tttccgtatt tatgtttagt tgtctttgtc gcaggtcata ttgcacgtta tcgttatgat
    12901 aaattttctt ggacagctaa atctagtgaa ttaatagaaa gaaaaaggtt gatgtgggga
    12961 agtttattat ttcacttagg tattattcca gtgttttttg gacacgtagt tggattgtta
    13021 ataccgaaac catggatgga tgcagtgggt gtgtctgagc atttatatca tataggcgct
    13081 gtttatattg gtagcatctt tggtataata actttgatag ggatgttttt attaacagcg
    13141 agacgtgtta ctacgaaaag tattcgaaga ttaagttcag cgtcggatat atttgtaaat
    13201 ttcttattat tacttatcgt atttatggga tgttacgcaa cattagtaac aaatatacaa
    13261 catccagact ttaactacag aacttcatta tcaatttggt ttagacaatt atttatgtta
    13321 aaaccagatg cgtcattgat gagtggtgtg ccgattgcat ttaaaatgca tatattgtta
    13381 ggtttcacta ttatggcttg ttggccattt acacgtttgg tgcatgtttg gagtgtacct
    13441 ttgacatata taaatcgcag atatattatt taccgtaaga ataaagtgta agcataagag
    13501 gtgaaaggat ttgacaccag aagcaataat tgaagatcgt agatttcaag agacattaga
    13561 taaaatacgc aaagaagagg gttatgattt tgcggcaatc gctttctatg aatctaacaa
    13621 accatcatct ccaataaaat ggcactatgt ttcaggtaat aaaaataatc gatttaaatt
    13681 gattatttta agaaaaggtc gtggattagc tggaactgtt atgaaaacag gaaagcgtat
    13741 ggtaatagcc aatgttggtt tagctttagg gcctgaagaa aagattgact atccaatttt
    13801 attgagcgaa tcattaacag cagttttagc agtgccgctt tggtataaga atcaagtgta
    13861 tggcgtacta ttatttggtc aaagagatgg tcgtccttta ccaaaaatat ttgataatga
    13921 tgatattcaa cgtaagtttg gtattttcaa tgatgataaa taatgagatg aattagggtg
    13981 taagtcatga ttaatgagga cagtatacag ttagatactt tattgaaaaa atattatgag
    14041 cattcaatcg aaaagatagt ttttgctgat gataacggta aaattattgc aatgaatgat
    14101 gctgctaaag atatattatc tgaagaagat aattacagtg ctgtggcgaa tgcgatttgt
    14161 cacagatgtg aagggtatac aaatgcctat gatgtacaat catgtaaaga ctgtttctta
    14221 gaatcgatgc aagtacaagc tacgaatttc caagtattta tgaagacgaa agatcaaaaa
    14281 gtgatgccat ttacagcaac atatcagttg attgatcaag acaggggaat tcatgcattt
    14341 acgcttcaaa atgtttcatc acaaattgaa cagcaagaga aattgcatca acaacgtatg
    14401 atgcgtaaaa caatctcagc ccaagaaaat gaaagaaagc gaatttctag agagttacat
    14461 gatagtgtga ttcaagaaat gctcaatgta gatgttcaat tgagattatt aaaatatcaa
    14521 gaagatacaa ctaagctttt ggaagacgca gaaaatattg aatatattgt cgctaaatta
    14581 attgatgata tacgtaatat gtcggttgaa ttaagacccg cttctttgga tgatctaggc
    14641 cttgaagcag cttttaaatc atattttaaa cagtttgaag aaaattatgg aataaaaatc
    14701 atatatacat caaatattaa aaatactcgc tttgatagtg atattgaaac ggttgtttat
    14761 cgagtagtgc aagaagctat tttaaatgcg ttgaaatacg ctgatgtaaa tgaaataaat
    14821 gtaggtatcc gtcaaactgg gcgccatttg gtagcagaag tgatagatgc gggaaatggt
    14881 tttgatccaa gttctaagcc taaaggttct ggactcggtt tatatggaat gaatgagcga
    14941 gctgagttag tcagcggtag cgtcaatatt gaaacgaaaa ttggtgaggg taccaatgtt
    15001 acattgaaca ttccaatttg aaacatacat tgggggaata aaattgaaaa tagtcattgc
    15061 cgatgatcac gctgttgtcc gtacggggtt ctctatgatt ttaaattatc aaaatgatat
    15121 ggaagttgtt gcaacggctg cagatggcgt cgaagcttac caaaaagtaa tggaatataa
    15181 acctgatgtg ttactaatgg atttaagtat gccaccaggt gagtcaggtc ttatcgctac
    15241 gagtaaaatt gctgacagtt ttcctgaaac taaaatacta atattaacaa tgtttgatga
    15301 tgaggagtat ttgttccatg tgttgcgtaa tggtgcgaaa ggttacatat tgaaaaatgc
    15361 acctgatgaa caattattgt tagctattcg aactgtatat aaaggtgaaa catatgtaga
    15421 tatgaaactt acaacatctt tagtgaatga atttgtatct aattcaaatc aggacactgc
    15481 aaacacaaca gatcctttta aaatcttatc aaaacgagaa ctagaaatat tgccacttat
    15541 tgccaaaggt tacgggaata aagaaattgc agagaaatta tttgtatctg tgaaaacagt
    15601 agaagcacat aagacgcata ttatgacaaa gcttggctta aagagtaaac ctgagctagt
    15661 tgaatatgca ttgaaaaaga aattattaga gttttagagt ttgaaattaa tataattcag
    15721 tataaaagcg cgagaggaaa tgattatctt ctggcgcttt tgtatgtttc gaaatatatt
    15781 tttttataaa tcgaatggaa cctaatcctt tctcctttcg gtaacctata ctaattcgct
    15841 ttgcaaaggt ataactttta atatagttat tgatttagag aataaatgat tagttttcta
    15901 ctttgaacaa ttattttagt tggtgtattc agtgttgtag catctgtatg aggattaaaa
    15961 attgtcgctc ttattcacat tgctcaaaaa ataattaaca gcttctgaaa tgtatgatgc
    16021 taaattttga ttatcatatg aattaaagta atctttgaaa cgcgtatcat cttcatatgt
    16081 tttagcaata cagcataatg tttcatcatc aaaatcagcg atagtgttca taaatgcctt
    16141 ccactcaaat acgagatcac tacaatcttg gattggataa tgactcaaag acatatgatt
    16201 aaatttattg aagatagttg tcaatttatg tctgatatcc tgcgattgta agctgtcttt
    16261 acgtctaata aatgattgat atgctttcgt atgaccatat tttatagatg cttctttatc
    16321 atattgtgac tgtagtgtct tgtttaaaat gctggtatct attagagaga gtcctttaat
    16381 aaattcacat acagagcgtc taattaattg taagtcagaa atctttttat ctaacttgct
    16441 tatttgctct gataataact ttcgcaattg ttcattatca tacgaaatgt attgttttat
    16501 tttagcgata tcaaaatcaa aagacttgag tattaaaatc ttttgtaatt tttctaagtc
    16561 ttgctgttta taaacgcgat aatttttatc atttttatct ggaactaata atcctatttc
    16621 atcgtaataa tgtaaagttc ttttagttac acctgtaatt tcaataatat cttttaaagt
    16681 gtatgtgtat gccaaaattc agtcacctca tttattattt tttgtgctgg aagtgttgta
    16741 gcaacttcaa taggtatgtt tgttttttgt aaaaagtatt ttaccaagtg atagccacat
    16801 gcatacccgg cagcatgaga taagccaacg ataggcctac cttgaagttt attaatatca
    16861 tcaccataga gataaggcat cgattcaaat atatgcttta aatgtaaatg attataaata
    16921 gtatttttaa tttttacgtt atcacgactt caattagtat ttgttaccca cggtccaata
    16981 tgcgctttac catacaatga ttctacatag ttttcagcta atccttctgc aataatcaat
    17041 tcaatcaaac ttccgccatc ccaatcaata tattgatagc gcatattatg atttacttca
    17101 tgtgcaatta agcttttcat agaattaatt gtacttgtac tcggcactaa ataaatcata
    17161 atataacctg gtatgccacc atcaccaccg cgatttttat ttagatacat aagtggtttt
    17221 tggcgatctc caagtaatac tgtgaaatga tagttagaaa cttgagatga gatactataa
    17281 tttgaaaatt gatctatagc aacttttaaa tatttttcgc aattactcca gaaatcatta
    17341 ttcaggtatt gtattgacat ttggtcagac atgcgtaaat ctttgggaga aatttgaaag
    17401 gcatctaaaa attgaatcgc actcattgtc tgtttgtcat cattataaat aggcatatgt
    17461 tgtacctcaa atttcttttt aaaaggtact aatatttctt tagtgaaaat tgaatcacgg
    17521 tcgtttattg gtgccttgag tatattatta tagacggaat ctgatctaat aatattgatt
    17581 ttatacatga taaacctcct tatgttgtca gcataaagga taacgtaacg tgattttcaa
    17641 gcagtaattg taactaattg aaaaaaatta agaaaagtat gtgagtgttc ctaaataata
    17701 tgattaaaat gatggcgaat aagtgtctaa aagcatctta aagggacatt gtatagggta
    17761 aatcacttca taaataaggg aaaatcctta tgttcacttt ttcacaatca taataaaata
    17821 tatatgtagt caatactttg tctatattga atgttttcat ataaatgaaa gcatttttaa
    17881 ataacattga cctctaatat ataggcagag tattgatatc tattaaaaaa taaatgattt
    17941 tgatgaaggt gaaacgtatg tacaaaacaa aaggtggctt tcaacttaca ttacaaacat
    18001 taagtttagt ggttgggttt atggcttgga gtataattgc gccattaatg ccctttatta
    18061 aacaagatgt caatgttact gaaggtcaaa tatcaatcat tttagcgata ccagttattt
    18121 tgggatcggt gctccgtgtg ccatttggtt atttaacaaa cattgttggc gctaaatggg
    18181 tattctttac tagttttatc gtattgttat tcccgatatt tttcttaagc caagcacaaa
    18241 caccgggtat gttaatggct tcaggatttt tccttggtgt aggtggtgca attttctcag
    18301 ttggtgttac atcagttcct aaatatttcc ctaaagaaaa agtaggtcta gcaaatggta
    18361 tttatggtat gggaaatatc ggtacagcag tttcttcatt tttagcacca ccgatagcgg
    18421 gtattattgg ttggcaaaca acagttagaa gttacttaat tattatcgct ttatttgcat
    18481 taattatgtt catttttggt gacacacaag aacgtaaaat taaagtacca ttaatggctc
    18541 aaatgaaaac attatctaaa aactacaaat tatattactt aagttattgg tattttatta
    18601 cttttggtgc ttttgtagca tttggtattt tcttacctaa ctacttagta aatcattttg
    18661 gaattgacaa agtagatgct ggtattcgat caggtgtatt cattgcgctg gcaacattct
    18721 taagaccaat aggtggcatt ttaggtgata aatttaatgc agttaaagta ttgatgattg
    18781 attttgttgt tatgattatc ggtgccatta ttttaggtat ttcagaccat atcgcattat
    18841 tcactgtagg ctgtttaaca ataagtattt gtgcaggtat tggtaacggc ttaatcttca
    18901 aattagtacc atcatacttc ttaaatgaag cgggatccgc aaatggtatc gtatcaatga
    18961 tgggtggttt aggaggattc ttcccaccac tagtaatcac gtacgtagct aatttaacag
    19021 gatcaagtca tttagcattt attttcttag cggtatttgg atgtattgca ttatttacca
    19081 tgcgtcattt ataccaaaaa gaatatggct cattgaaaaa cggttgatat gtaatacatg
    19141 ccattcattt agttaaatac aaagccttaa tatcatgcgc aatattcgta gcatgacatt
    19201 aaggctttag tagtgtattc attttaaatt taactatgaa attggaatag atgttgatga
    19261 agtagtttca ttgcttggtt tgattttagg caaggtaatg gttaataacc cattttcaaa
    19321 actagcagta atatgttgct tatcaacagc ttcaaaatca aattgacgca ttaatgattc
    19381 gaagttacgc tcatctaaaa tgagttgttc agatttgtat tttgcgcttc tagtagcttg
    19441 aatagtgagc gtattattat tgaaatcgat actaatatct tctttattta cacctgctag
    19501 ttcagcttct aagtaatata attcgtctgt ttcatatata ttcgttacaa atgaaggtgt
    19561 tgaaaagtaa ttaaatactt gtttacctaa atctttaaat gtatcacttg gattagcgtt
    19621 aaaaaagttt tgattctcga attgattgaa gttcaatatc atcacttcca ttatagaaat
    19681 ttgtattaca aagccattat atgagtagta ttaaaaaagt taaagtaaaa ggggagataa
    19741 aaaagtaata aatgggtaga tttgtgcctg ataaggaatc agggcaaaag aactgagcat
    19801 cgagatttat acaagcaata aagataatga ataaacatat cgatgcatac ttctgcccat
    19861 caccttatga tagggagtta ttttttgtcg gaatagtcaa aatttttaga aacaaaattc
    19921 cgcaaattat taattatatc tgatgcttct cgttctgaaa tgttgaattc attaaagacc
    19981 ttaacagaaa tttcagcaag agggcttttt attgctttac cttgttctgt tagagaaatt
    20041 tgtaggtttc tttcatcttt ctcttcacgt gttcgaacaa cgtaatcttt cttttctaat
    20101 ttctttagta atggtgtcag tgttccagaa tctaagaaca cacgttcacc taattttttg
    20161 atgttaagtt tttcatcatt ttcaatcgcc attaaaacaa tgtaaccagt ataggttaaa
    20221 tcatattcct taagatagtt tgtgtatttt ttaattattt cttttgaaga aacataaaat
    20281 aaaaaacaca actgtttgct aagatagctg ttttctacat acatccaatc actccttgtt
    20341 aaaataaaca atattatgaa gtgtaatagt tgtcaagtac tgatttcatc gcaattattg
    20401 aattgtctat aattgaattt aaatgttaaa taatgcaaaa tatatcgata tcttagtatt
    20461 ttcatcggta attactgata tgactgattt tttaaattga aaaaaatgta attgtatcat
    20521 ccttaattgt taaaagtagt caattatttt gatagatagt aaaataaaat ttgttattaa
    20581 attttttaaa agcatcgtgt atgtaattcg atttctataa taatattgag aataaaaaaa
    20641 tttgttacaa tctggtaaaa actctttatt aaataaattg attcgttcat actgaataga
    20701 taagcaaaaa ggagttacat aatgaagaag aaaaaaaacg cgattaaagt tatcattata
    20761 ttatttattt cattaattgt tgcagtagca tttttctttg gtctgaaaac atatcaaggc
    20821 cataagaata ttcaactaat tgacagttac ttggaagaga aaaatctaaa agataaaatt
    20881 aaatcagaaa aaacagagta tagtgctaaa aaaggtctct tctataagga agttacattt
    20941 aaagatgaac ctggtgtaac ttatgttgtg caaccaatca gtactaataa aggattattt
    21001 gttgaaggct ttgatacaga gactaagaaa agtttgaaaa ctgcaaagca taaatatttc
    21061 aatcaaaatt ataaaccatc aaaatagtga aacgttaaac tggtgaagca gtttagtgcc
    21121 ttcaatttta attatttgag gtaaacagtt tttgtatgga aattagtgat ttttgacatc
    21181 tataaaagat aaatatttta attttttata gataattatc aattttctga tataattgag
    21241 tgatgtcaga tgaaggggag tttgtactat ggctctattc aagagaaaaa ttagcctacc
    21301 tatgcaagtt gttattgcat tagtgttagg tgttgtcgta ggacttttat tatatggaca
    21361 agaaaacgta gcaaattaca ttaaaccatt tggtgacgta tttttaaatt taattaaaat
    21421 gatcgttata ccagttgtat tttgctcact agcgctttct atttcgaacg ttggggaatc
    21481 gaaaactgta gggcgttatg gctggaaaac aattttatac tttgaaatta ttacaacaat
    21541 cgcaataggt ttagggatta tcttcggtaa cctatttaaa ccaggtgctg gattagaccc
    21601 aacaaaatta cctaaaggtg atatttctaa atatcaatca actgcacatg cagcagaaca
    21661 atctacatat ggaaatcatt ttattgatac cattgtacat attattccga caaacttttt
    21721 tgaagcttta aataagggtg aattattacc tattatcttc ttcgcagtat tctttggatt
    21781 aggattagct gctgtaggta aaaaagcaga accagttaaa gaatttttaa gcggatcgct
    21841 tgaagctgtg ttctggatga ttaataaaat tttaaaatta gcaccacttg gagtgtttgc
    21901 attcatttgt actacaatta ttacatttgg tgcatccgca ttattaccac tattaaaatt
    21961 agtattagtt gttgtctttg caatggtgtt ctttgtattc gctatactag gactagttgc
    22021 atggatgtgt ggtattaata tcatgaatat tattagaatc ttgaaaagtg aattgctttt
    22081 agcattttct acatcaagtt cggaagctgt acttcctgta atgatgaaga aaatggaaaa
    22141 cttcggttct ccaaaagaaa ttacttcttt tgttatacca attggttata cgtttaactt
    22201 agatggatca gcactttatc aatctattgc agcattattc gttgcacaga tgtatggaat
    22261 gcacttaaca ttatcagagc aaattgtgtt gatgttaaca ttaatgatta cgtctaaagg
    22321 tatggcggca gtaccaggaa cttcaatcgt tgttttatta acaacattag gtgccatggg
    22381 cttaccggca caaggtttag cattaattat tggtgttgac cgtatcttag atatggtacg
    22441 tacatgtgta aacgttattg gtaatgcatt atcaacaatc gttatagcta aatgggaaaa
    22501 cgtatatgac aaagcaaaag gtcaagaata tttaaaatca atttaaaaaa tactatctga
    22561 catttaaagc cccttacaac ctttggttgt taggggcttt tatgcatgcg ctaaagcgca
    22621 ggcgaacgga agcgtagtga cctcaggttg ttagggactt ttatgcatgc gctaaagcgc
    22681 aggcgaaccg aagcgtagtg acctcaggtt gttaggggct tttatgcatg cgctaaagcg
    22741 caggcgaacc ggagcgcagt gacctcaggt tgttaggggc ttttatgcat gcgcaaagcg
    22801 caggcgaacg gcaagtggag ctattcaata tgaaagtatt tcaaatataa aaagcagcag
    22861 taaaatgatt ccaaattgga aatcaactta ccgctgcttt tactcgtata taccttgatg
    22921 gcaactaaaa tttgtaattt aaattacaat tagtgtttca tgtgcatttt gcccatgtca
    22981 tgaattttca tcattaaacc atgtggtaca tcgtcatggt caactttttt aactttaaca
    23041 attttatctg aatctgctaa tttataaaca attacaaaat cacctttttt aactttaaca
    23101 tctgaatctg aaggtaattt aacatttttt tcaatacttt catctttttc actaatcatt
    23161 ttttcaatag tagtattatc tttcataatt ccatagtatg tatctttctt tccgccagtc
    23221 atcatcatga ttactaatac aatacataca attaacatga tggcgattaa actaatacca
    23281 agaccaaaac cttttttctt tttcatggtt catcaatctc cttaaatgta gttaattctg
    23341 ttatacctaa aagtgcaaca gtttgcaaat gcagtattta tattacataa aattaattta
    23401 aaaagacaca ccctaatcac gttaaatgtg aataaagtgt gtctaaaaaa caatacattg
    23461 caaat
//
LOCUS       DAKTGS010000027        23404 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.027, whole genome shotgun sequence.
ACCESSION   DAKTGS010000027 DAKTGS010000000
VERSION     DAKTGS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23404
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.027"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(80..1207)
                     /locus_tag="O0286_000914"
     CDS             complement(80..1207)
                     /locus_tag="O0286_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0169300.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1569..1946)
                     /locus_tag="O0286_000915"
     CDS             complement(1569..1946)
                     /locus_tag="O0286_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCY0169301.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2362..2955)
                     /locus_tag="O0286_000916"
     CDS             complement(2362..2955)
                     /locus_tag="O0286_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCY0169302.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            3019..3402
                     /locus_tag="O0286_000917"
     CDS             3019..3402
                     /locus_tag="O0286_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCY0169303.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3533..5938
                     /gene="mnhA1"
                     /locus_tag="O0286_000918"
     CDS             3533..5938
                     /gene="mnhA1"
                     /locus_tag="O0286_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCY0169304.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5931..6359
                     /gene="mnhB1"
                     /locus_tag="O0286_000919"
     CDS             5931..6359
                     /gene="mnhB1"
                     /locus_tag="O0286_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCY0169305.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6359..6700
                     /gene="mnhC1"
                     /locus_tag="O0286_000920"
     CDS             6359..6700
                     /gene="mnhC1"
                     /locus_tag="O0286_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCY0169306.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6693..8189
                     /gene="mnhD1"
                     /locus_tag="O0286_000921"
     CDS             6693..8189
                     /gene="mnhD1"
                     /locus_tag="O0286_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCY0169307.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8191..8670
                     /gene="mnhE1"
                     /locus_tag="O0286_000922"
     CDS             8191..8670
                     /gene="mnhE1"
                     /locus_tag="O0286_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCY0169308.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8670..8963
                     /gene="mnhF1"
                     /locus_tag="O0286_000923"
     CDS             8670..8963
                     /gene="mnhF1"
                     /locus_tag="O0286_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCY0169309.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8941..9297
                     /gene="mnhG1"
                     /locus_tag="O0286_000924"
     CDS             8941..9297
                     /gene="mnhG1"
                     /locus_tag="O0286_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCY0169310.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9546..10700
                     /locus_tag="O0286_000925"
     CDS             9546..10700
                     /locus_tag="O0286_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0169311.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10756..11130)
                     /locus_tag="O0286_000926"
     CDS             complement(10756..11130)
                     /locus_tag="O0286_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCY0169312.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11149..12465)
                     /locus_tag="O0286_000927"
     CDS             complement(11149..12465)
                     /locus_tag="O0286_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCY0169313.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12876..14351)
                     /locus_tag="O0286_000928"
     CDS             complement(12876..14351)
                     /locus_tag="O0286_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCY0169314.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14482..15690)
                     /locus_tag="O0286_000929"
     CDS             complement(14482..15690)
                     /locus_tag="O0286_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0169315.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(16144..16503)
                     /locus_tag="O0286_000930"
     CDS             complement(16144..16503)
                     /locus_tag="O0286_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCY0169316.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(16516..16752)
                     /locus_tag="O0286_000931"
     CDS             complement(16516..16752)
                     /locus_tag="O0286_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCY0169317.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            17069..18133
                     /locus_tag="O0286_000932"
     CDS             17069..18133
                     /locus_tag="O0286_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0169318.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(18192..18515)
                     /locus_tag="O0286_000933"
     CDS             complement(18192..18515)
                     /locus_tag="O0286_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCY0169319.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            18616..18858
                     /locus_tag="O0286_000934"
     CDS             18616..18858
                     /locus_tag="O0286_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCY0169320.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(19121..20296)
                     /gene="dltD"
                     /locus_tag="O0286_000935"
     CDS             complement(19121..20296)
                     /gene="dltD"
                     /locus_tag="O0286_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCY0169321.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(20293..20529)
                     /gene="dltC"
                     /locus_tag="O0286_000936"
     CDS             complement(20293..20529)
                     /gene="dltC"
                     /locus_tag="O0286_000936"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCY0169322.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(20547..21761)
                     /gene="dltB"
                     /locus_tag="O0286_000937"
     CDS             complement(20547..21761)
                     /gene="dltB"
                     /locus_tag="O0286_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCY0169323.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHTALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(21758..23215)
                     /gene="dltA"
                     /locus_tag="O0286_000938"
     CDS             complement(21758..23215)
                     /gene="dltA"
                     /locus_tag="O0286_000938"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCY0169324.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(23231..23383)
                     /locus_tag="O0286_000939"
     CDS             complement(23231..23383)
                     /locus_tag="O0286_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCY0169325.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
BASE COUNT         7462 a         4096 c         3557 g         8289 t
ORIGIN      
        1 aagaatattt ttatttataa atattgaaaa ccactaacaa attgcataca caatatcatt
       61 agtggtaaca gttaaacact tatttatctt tacgtggtaa tggataaaac ccttcattaa
      121 attgatgcca taaattagga gttaagtgat gattatcttc tctctctgga tcaaagtaat
      181 taataatttc atcttcccgt ccatctttaa ttttttcaac aaattgataa tccagtagta
      241 gctctctacc aatggctact aagtcaacac caacattttc aactgcatct aaagcttcgt
      301 cagctgtgaa aattgaacca ataccgataa gtggcatacg accatttatc catttgtgaa
      361 ttaaaggcag tctttcttgt ccagcgtatt taccttcacg tgtcgttgca tgcgtatcca
      421 tcattgaaac atgaatatag tcgattggca tatggctaat tttattaacg agttcctctg
      481 taatttccat tgtgattcct ggagactccg cttcctctgg agataatctg tatccaacta
      541 taaagtcttt attgccatac gcttctttcg ctttaagtac ttcttcaatc acagcgaccg
      601 ggaatttata ttgatttgcc catacatcat ttcttctatt atagtatggt gatacaaatt
      661 gatgaattaa gtaatgattc gcgccatgta tttcaacacc atcaaaccct gcttcaattg
      721 cacgtcgcgt tgcttcacca aaatccttga ttgcttgttc aatctcttca ttcgtcattt
      781 ctctagcact atgttcttgt ttctgaccaa aactttttaa agaaattgga cttggtgcta
      841 cgacgtctcc atcaggtgtt aattcaggca atgcttgtgc accgccatga tgtatttgta
      901 cgagtgcttt ggcaccgttt ttcttcattg ctgtagctaa tcgttttagt ccttcaatat
      961 tactgtcatg cgcgattgat ggctgtcctg gaaatgcttt tccgacatca ctcacattac
     1021 tcgcagcatt aattgtaata ccaacatctt gtgaacgctt ttcaatataa ggaagttcta
     1081 catctgaaat agtaccatca tcatttgaag aaatatgtgt taaaggggct aacacaaatc
     1141 gatttctcaa ctctactcca tttggtaatt ctactttatc aaacaatggt tcgtatttac
     1201 ttttcattac ttaattcctc cttaaaatta ttgagttgac tataacatgt aattttttca
     1261 ttctccaatt atctgtttat atagttgcaa ttcgtttaat actttaagtt gtagttctca
     1321 tttctaaaat aaataaaaaa aacaaatcca catacaatat tgcaaatcaa cgattctatc
     1381 taacaaagat aatggatatg tcaccttaaa ccatttgtac gtgtaaatag aatcaatcat
     1441 attacgttat cgtagacaaa aagagattag tatctaataa aatttaatag atactaatct
     1501 cttttatagt ctaagaaact ttcatttcag accctttgta tgattttcgg tacgatttat
     1561 ctgttccttt agtcgtttcg aattgctcgc tttgactgtt ttatccagat tggtaagcgt
     1621 tctttaagtg tttgaaaccc atatttttct gtttctctga tttcatctaa tactgattgt
     1681 ttttccttct tacgctcata atttttgaag taatcattat cctttaatga tagattaagc
     1741 tttccttcat catctataga caaaatttta gctttaacaa tttggccttc tgatagaaat
     1801 ttcttcaaat tatgaacgta gtcatccata atttctgata tatgaatcag tccttcagta
     1861 tgattagggg tctcaacaaa cgcaccgtat ggttgaatac cagttacacg caccttgata
     1921 tgttggccaa ttttgtagtt attcaactta ataacccctt atttcgttta cttaacacta
     1981 ttatcatagc acaaatcgaa atggtggact aattaaaaca tcaaatcata cgaatattat
     2041 tttataaatg aataagaatt ctacaaactt ggatttacaa ataaaattag caacaaattc
     2101 ctatctaaat caatatgata tccagctact ttgttttcaa tttatttact atttgaatag
     2161 caattctctg taaaaacata aaaagcaacc tcaactatta tctttaaaat acacatctat
     2221 ttaaaaactg tgctttcttg ataaatatat tgagattgct catcatatta gtttatttat
     2281 tttcgttgga atatatacta atccattcat aaattagctt tatccgagtt taaaatgttg
     2341 gtagttaatt atgtttagat attattcttc aacatcaata gattcaatta caacatcatg
     2401 aagtggttta tcttgtggtc ccacttttgt atttgcaata tcttctaatg tagtttcacc
     2461 atcaatgatt tgaccgaata ctgtatgttt ttgatctaac catggtgtac cacccttttc
     2521 gccatatgca tcaacgattg gttgaggcca gccaccatct gcaagttgac ttaacatatt
     2581 ttgaggtact tctttcattt gaacaatgaa aaattgtgaa ccattagtat taggtcctga
     2641 gttagccatt gataatgcgc catataagtt aaatgcattt aatgaaaatt catcttcaaa
     2701 agcaccgcca taaatacttt cgccacccat accagtagct gttggatcgc caccttgaat
     2761 catgaagtca ttaatgacac ggtggaatgt gattccatca taataaccat tttttgcatg
     2821 tgtcacaaaa ttttcaactg tttttggtgc aatatttgga aataatttga atgtcatgtc
     2881 acctttattt gtgtgcataa ccactttgat ttcaccttgt tgtacttctt tgtttaactg
     2941 tggatagtta gccatttata ttctccattc atgttatgat acatatatat taacatactt
     3001 ttaagggagg aaaatagcat gaagctatat cgattttcac acaaaacagg tagctatggt
     3061 gtcacgattg ttgaagaaaa tggagacgat gtccttgtta aagttgaaca agttatcaaa
     3121 cacccgaaac aaggtgattt acataaccct actgaaactg aaggtgtatt tttccatgac
     3181 cgcaaagcat taagccattt tgaaaaacgc tatacgaaac gttcacaatt acgcgacttc
     3241 aatgttgatg tattgcgcta tgaagattca cttcaacaag caattacaga tttagaaact
     3301 aaacttaaag ctcagcaaac aaaacatgct gaaatgtctt tagctagttt agcacgctta
     3361 aaagaagatt attcacttca atacaaagaa aattttaaat aaaaaatgac gtgtttcttc
     3421 ttatttaaat acttctgaaa ataaagcaca acaaggagtg tgtacgtttt tcagaactat
     3481 atcattcatg tataatgaat aacataaata ttttttaaag gaggacaaca tagtgagttt
     3541 gttacatatt gcggtgatat taccgttaat attcgcactc attattccta tcctgtatag
     3601 attttttaaa cgtatacatt taggatggtt cgtattacct gtccctatcg tcatatttat
     3661 ttatatgctt acattaatta aaacgacgat gtcaggtaat actgtaatga aaaccttaaa
     3721 ttggatgcct cattttggta tgaactttga tttatatctt gatggcttag gtttactctt
     3781 tagtttgctt atttcaggaa tcggcagttt agtagtatta tattcaatcg gctacttaag
     3841 taagtcagaa caactaggga atttttattg ttatttatta ctatttatgg gtgcaatgtt
     3901 aggtgtcgta ctttcagata atgttattat tctatattta ttctgggagc taacgtcatt
     3961 ttctagtttt ttacttattt cattctggcg cgaacgacaa gcatctatat atggtgctca
     4021 aaaatcgctc ataatcacag tatttggtgg tctatcatta ttaggtggta tcatcttact
     4081 cgctatacca acacaatcat ttagcattca gtacatgatt caacatgctt ctgaaattca
     4141 aaatagccca ttcttcattt ttgcgatgat tttaatcatg attggtgctt ttacaaaatc
     4201 tgctcagttt ccattttata tttggctacc agatgcaatg gaagcaccta caccagtcag
     4261 tgcttatctt cattcagcaa caatggttaa agcagggtta tatttaatcg ctcgaatgac
     4321 accaatcttc gcagcatcgc aaggttgggt atggactgtc acactcgttg gtttaattac
     4381 attattctgg gcttcgctaa acgcaacaaa acaacaagac ttgaaaggta tactggcatt
     4441 ttcaacagta tcccaattag gtatgattat ggcgatgctt ggaatcggtg ccattagtta
     4501 tcactatcaa ggcgatgata gcaaaatata tgctgctgca tttacggctg caatattcca
     4561 tttaatcaac catgcaacat ttaaaggtgc cctatttatg attacaggtg cagtggatca
     4621 ttctactggt acacgtgatg ttaagaaatt aggtggctta ctaacaatta tgcctatttc
     4681 atttacaatc actgttatta cagcattaag tatggctggt gtaccaccgt tcaatggttt
     4741 cttatcaaaa gaatcatttt tagaaacaac atttacagca agccaagcaa atttattcag
     4801 tgttgataca ttaggctacc tattcccaat cattggtata gtgggcagtg tcttcacatt
     4861 tgtatattca attaaattta taatgcatat atttttcgga caatataaac ctgaacaatt
     4921 acctaaaaaa gcacacgaag tatcaatatt aatgctttta tcaccagcta ttttagctac
     4981 attagtaatt gtatttggtt tattccctgg cattttaact aattcgatta ttgaaccagc
     5041 tacatcttcg attaatcata cggtaattga tgatgttgaa ttccatatgt tccatggatt
     5101 aacgcctgcc tttttatcaa cattagtgat ttatatttta ggtatcttac taattgtgac
     5161 atttagttat tgggtgaaat tgttgcaacg acaacctggt aaactaacat tcaattattg
     5221 gtacaacaga agtgcaaatg ttattccaaa ctactctgaa aagatgacga atagctatgt
     5281 taccgattat tcacgtaata atttagttat catttttggt gcattaattt tattaacatt
     5341 tgtcactata ttctcagtac ctttcaacat taattttaaa gacgttagtc cgattagaat
     5401 tttcgaagtt tgtatcgtaa ttttattatt aagtgctgcc tttttaattc ttttcgctaa
     5461 atcaagatta tttagcatca ttatgttaag tgcagttgga tatgctgtgt ctgtattgtt
     5521 tatattcttt aaagcacccg acttagcatt gacacagttt gtcgttgaat ctatttcaac
     5581 agcattgttc ttattatgct tctaccactt accaaattta aatcgttata atgaaaagcg
     5641 ctcattccag cttactaatg ctttaattgc tggtggtgtt ggtttgtccg tcattattat
     5701 cggtttaatt gcatacggaa atagacattt tgaatcgata tctaagtttt atcaagagca
     5761 cgtttatgat ttagctcacg gtaaaaacat ggtaaacgtc atacttgttg atttccgtgg
     5821 tatggatact ttattcgagt catctgtgct tggtatcgca ggattagctg tatatacaat
     5881 gattaaatta cgtaaaaagc gtcaaacgca aggtaacgag gtgaaaaatc atgaatagac
     5941 aacaaaatga tctaattttg caatttgctg cagttattat ctttttcatg gtaatggtct
     6001 ttggtttttc acttttctta gcaggacatt atacacctgg tggcggtttc gtcggcggat
     6061 tattattcgc aagttcactt gttatcatta cgattgcatt tgatatagaa acaatgcgta
     6121 aaattttccc attagatttt aaaatcttga taggtatcgg attagttttt tgtattgcga
     6181 cgcctatcgc aagttggttt ttaggcaaaa atttctttac gcatgtcaca tttgatatac
     6241 cgttatttat attagaacct gttcatatga caacagcagt attctttgat ttcggtgttt
     6301 tatgtgcagt agttggtact gtcatgacaa ttattatttc gattggagag aacgaatagt
     6361 ggaaattatt atgatttttg ttagtggtat tctcacagca attagtgtct atctcgtttt
     6421 gtctaaaagt ctgatacgaa ttgttatggg aactacacta ttaacacatg cagcaaattt
     6481 atttttaata actatgggcg gacttaaaca tggtactgtt ccaatttatg aagcgaacgt
     6541 aaaaagctat gttgatccta tcccgcaagc acttatttta acagcaatcg ttatcgcctt
     6601 tgcgacaaca gcctttttct tagtattagc atttagaaca tataaagaat taggcacaga
     6661 taacgttgag agtatgaaag gagttccaga agatgattga aagtaatatg cttgtgttaa
     6721 cgctagttat tccagtaatt acagccatat tacttgtctt tattggaaag cgtcctatta
     6781 taaagcgtta tgtcgcacta ggcggtacat tattaacctt ggtcgcggca attatcaatt
     6841 tagcaaatgt tgttaaacat ggaccaatac gcgtagagct aggttcatgg aaagcaccat
     6901 acagtattgt ctttgtatta gacatattca gtgccctact aataattact agtattatta
     6961 ttacagctat tgtgatttta tattcatacc aaacaattgg tattgaacgt gaaagatatt
     7021 attattattt ttcagtacta tttatgttaa taggtatcat tggtgcattc acaactggtg
     7081 acattttcaa cctattcgta tatttcgaag tatttttaat gtcatcttat ttcttattag
     7141 taattggttc tacaaaaatt caactacaag aaacaataaa atatgttctt gttaacgttg
     7201 tatcgtcatc cttcttcgta atgggtgtag cgattttata ttcagtagta ggaacattga
     7261 atctagcaga tataagcaat aaattagcta acctttcagc gcatgacagt ggcttagtca
     7321 atatcgtttt catactattt atctttgttt ttgctacaaa agctggcgta ttcccaatgt
     7381 ttgtttggtt acctagtgca tactatgcac caccaattcc aatcattgca ttctttggtg
     7441 ctttgttaac aaaagttggt gtttatgcaa tcgctagaac attgagccta ttctttagtg
     7501 ataatgtaag cttttcacat tatgtcattt tattcttagc actattaacg atcatctttg
     7561 gttgtgtcgg tgctgttgca tatgccaaca ttaaaaaaat catcctatac aatgtcatga
     7621 ttgctgtagg tgtgatctta gtcggcgttg caatgatgac agagtcaggt atgattgggg
     7681 ctatttacta tacattgcac gacatgttag taaaacttgc attattcttg cttatcggaa
     7741 ttatgattaa aattacaggc actgctgatt taagacaatt tggtggctta attaaacgat
     7801 atcctgtact tggttggtca ttctttatcg cagcattaag tttagctggc attcccccac
     7861 tcagtggctt ttatggaaaa ttttttattg tccaatcaac atttgaaaga ggattttatt
     7921 taagtggtgt catcgtctta ttatcaagtt tagtcgtatt atattctgtc attcgcatat
     7981 tcctacaagg ctttttcggt caaccaaaag gatatgatct taataacaaa gtcgacgtca
     8041 aatatttaac tacgattgca atcgttgcag ttgtgataac tgttttatat ggattgtcag
     8101 cagattactt atatccaatg gtaaaagctg gtgcagaaac attctacaat ccatcaacct
     8161 acgttaaagc tgtattagga ggtaaataac atggctgtac aattagtatt aaactttatt
     8221 atcgcagtgt tttggttgtt tgtgacaaat agttacacaa caaataactt tgtgttaggt
     8281 ttcatctttg gcctagttct tgtttacctc ttacacagag tgttaccagg tagattttat
     8341 gtcatcacgc tatatcgtat tattaaatta gttattattt tcttaatcga actgattaaa
     8401 gctaattttg atgttttgaa aattattatt aaaccttcga ttaaaaatga accaggattc
     8461 tttgtttatc acacagattt aaaaaaagat tggcaaattg tcttgctgtc taatttaatt
     8521 acattaacac ctggtactgt agtgcttggt gtaagtgacg atcgtacaaa aatttatatt
     8581 catgccatag atttttcaac taaagagcaa gaagtagaaa gtattaaaac atcattagag
     8641 aaaattgtaa gagaggtggg cgaaatataa tgaatcataa tgttattatc gttattgcat
     8701 taatcatagt tgtcatttct atgttagcta tgctcattcg cgttgtgcta ggcccatcac
     8761 ttgccgatcg tgttgtcgca ttagatgcga ttggtcttca attaatggca gttatagcat
     8821 tattcagtat tttattaaat attaaataca tgattgtcgt tattatgatg attggtatat
     8881 tagctttttt aggtactgca gtattctcta aatttatgga caaaggtaag gtgattgaac
     8941 atgatcaaaa tcatactgat tagtcttgca cttatctttg ttatcatcgg tgctttaatt
     9001 agcgccctag cagctatagg attattgaga cttgaagatg tatattcacg tgcacatgct
     9061 gccggaaaag catcaacatt aggtgcaatg tcattactat ttggtacgtt tttatatttt
     9121 attgctactc aaggttttgt aaatatgcaa ttaatcgttg cgattatctt tgtattaatt
     9181 acaggtcctc tttcaagtca tatgattatg aaagcagcat ataatattaa aacgccttat
     9241 actaaaaaga ctaaagtcga tgaaatttcg gaagacttaa aagacacaaa attgtagatt
     9301 atataacaaa atcatgaata taaatcaaca acaaacagca gtaagatgat tccaaattag
     9361 gaatgatttt actgctgttt tcttttgaca ttgttacctc tttttcaatg attttttctt
     9421 tgactacaga ttcgccctat ctacatatat ctctttaatt taattgcctt tcatgtcgtt
     9481 atgtattatg ataataataa ttataaatcg taacgattac gttttaaaaa gagagaggtt
     9541 ttattatgca ttggacaatt atcggcggtg gcatacaggg aactgcaatc gcacaaaaac
     9601 tattatcaag cggattaaca acagaccgat taacaatcat tgacccacac gaaacttttt
     9661 gccaaaggtt taactcatat acaaatcgaa tagaaatgcc ttatttaaga tcaccgattg
     9721 tacatcacgt acatccacaa ccgttccatc taaaacaatt cgctaaacag caccaatata
     9781 caaatgcttt ttatggtcct tatcaacgac ctgaattgac aatgtttatg gatcatattg
     9841 cacatgcttc taaacaatat caattagagg attgcttggt tcaaggttta gttcaaactt
     9901 tagataaaca agaagacaaa tggcatatca agttagaaga tggacaaatt atcactacag
     9961 attgcgtcgt tattgcaata ggcagtacaa atattccgtt tatgcctgac attttaaaag
    10021 acaaacagaa tgtaaatcat atcttcgaga aagaacttga tcaagtagta tatgataaga
    10081 ccgatcatat cgttggtagc ggcattactg ctgcacatct tgcacttaaa ttgttaaatc
    10141 atgataacga taaaaagatt catttatggc taaataaaga tattgaaata catgactttg
    10201 atgctgatcc tggttggtta ggtccgaaaa atatgtcttc atttttaagt actaaaagca
    10261 tgcctgaaag aaatgccatt gtacaacgcg aacgtcataa aggatcaatg cctcacgaac
    10321 tgtacttacg ccttaaaaaa catattaaaa atggtcgtat aaatgtgcat aaaacaccta
    10381 tcactcaaat tagtggtggt gtaattaaca ctgaaaatga ttctgttcca tatcaacaga
    10441 ttatggttgc aactggtttt gaacaagatt ttatgtcaca accacttatt aagcaattaa
    10501 tacaaaatta tgatgcacct atcaacgaat gtaattaccc tgttatttcc gaaaaattag
    10561 aatggatacc aaatctattt gtcgcaggat gctttgcaga cttagaatta ggaccatttg
    10621 gtagaaatgt tatgggtggc cgtaaagctg ccgaacgcat tgaacaagca tttctaaaac
    10681 tacaacaata tagcgcataa aaaaactgct agccgtagtt atgaatatct cataacattt
    10741 cagctagcag ttcttttatt ttaaaggttt aatagcaact gtaccacgca taactgtaat
    10801 taattgttct ttgtcattct taatttttat atcccataca tgtgtcgact tacctcgatg
    10861 aataatttca gctgtcgcag taacacgacc atctttagca gaatgaatat ggttagcatt
    10921 catctctaaa cctaatggaa taaatttggt tgtatcaatt aaattagcag atcctaatga
    10981 acatgctgtt tcacctaaag caatcgaagc cccaccatgt aaatatccaa atggttgttt
    11041 tactttatca gtaacaggca tagaaataac aactaaacca tcttcctggt gatctattga
    11101 catctcaaat gtctctaata aatgagtcat attttcacct ctttatgctt atagtaccat
    11161 agcagcaata gtaccgaaaa tcattaaagg aatattataa aacaagaagt ttggtacaca
    11221 tgtatcacgt atatgatcat gttggccatc aacatttaat cccgcagttg gtcctaatgt
    11281 tgaatcactt gcaggcgaac ctgagtcacc taatgcactc gctgttccaa tcaatgcgat
    11341 taatgccatt gtatctagtc caattgacgc tccaaaagga atgaataatg atgcgataat
    11401 aggaattgtt gcaaatgatg atccaatacc taaagtgaca attaaaccta tcacatacat
    11461 catgataatg ctaaataatt tattatcacc agtaatactt gttaaagttt taactaattc
    11521 atctatatca ccagtagcat tcattacacc agcaaatcca tttgctgtta aaataactac
    11581 accaatataa gccataattt taataccttc aacaaactta gcatctaatt cataccaatt
    11641 atatgcacgt gaaataaaga atacgagtac ccctgccagt gcaccaaaaa tcattgaatc
    11701 tgtaaatgtt tgtactaaaa atgtagctag tattgctaca attgttacta ttaagatata
    11761 tggttttaac tctgtaacat tgtcactatc tgaaatttta cgtgtttcat attcacgtgg
    11821 tttacgatat acatataaac cgataagtaa gccaacaata taccccattg aaggaataag
    11881 cattgctttc caaatcatat taaactcaat tgggtgattt gcctttgcaa agccactttg
    11941 aataatttgc tggaaaattt gaccgaatcc atatggtaat aacacatacg ggaaacataa
    12001 accaaaaccg ataatcaaac cgattaaacg tctatctatt tttaagtcat taaacagact
    12061 taacaatggt gggatgacaa ttggaatgaa tgcaatatgt acaggaatta agttttgact
    12121 catgatactc atagctaata atgcaatgat tattgtcact ttgactttaa ctcttgacca
    12181 tcgactattt tcagcgtgaa ttgcatttat aatttttcct actaaatagt ctgtgatacc
    12241 actgtatgaa attaatgctg caaatccacc taataaagca tagcttaatg ctacctcagc
    12301 accatcgact atatttttcc caaatacatt tataactttt tcaatgctca tgcctgaaat
    12361 taagccacca actagcgcac tgataaataa gcttataact acgtttaatc gacataaaca
    12421 tagcacaatc attaaaatta ctgctattac tactgcattt atcatatgac aaccatcctt
    12481 tatcactcta tcgaactaaa gcgtttatag actacatagt atcagtagct ttaacacctg
    12541 tcaatagcaa actataccaa taatctttat tttttagtta attgtctttc ttaaaataat
    12601 tttagctttc attaaattaa acaattttac aagcttggaa caccaatcaa aatcctaagt
    12661 tctaaaatgc aatattagta gtcgttgact gaatgaacat atgcttataa tatttttttg
    12721 caatgctagt caagttgatt tatgctcaca aggatatgcg atttatattt tcttacaaca
    12781 atgaaaatgc ctgatacaat gcgatccttt atactttcaa gagttaaatt agaaatgtat
    12841 atcaagacac aagttgtatc aggcaaataa ttaaattatt gttgttttaa ccattgtact
    12901 atcgtcggaa tcataaatcc tgttggccct tttggaccat tatatgaagc tttattagtc
    12961 gttgctggac ctgcaatatc gaaatgaatg tgaggtgttt gaccactaaa atgtgttaca
    13021 aaacttgccg caaatagcgc tttaccttgt ccattcgtat ggttaactaa atcagcgata
    13081 tcactgtgtt taatacttgc acgttcggtt gcagtaatcg gtaattcaaa taccatttca
    13141 tcgacttcag aacttatttg taatatatcg tttaatatca ctttactatt cgattcaaat
    13201 gcagcagctt tatcatcgcc tagtgcaaca attgctgcac ccgttaatgt agcaaagtcc
    13261 ataatcacac taggctgata ttgatttgca taaaacacag catctgcaag gactaatcta
    13321 ccttcagcgt ctgtattcat tacttctaca gtttcaccac ttaatgctgt aaatacatca
    13381 tctggcttca ttgatgcttc atttatcata ttttcagcac acgcaagcac tccgacaata
    13441 tttacaggca gttgtaaacg actagccgct tcaatgatac caacgacatt cgcagcgcca
    13501 cacatgtcaa acttcattgt agccatgcca ttcttcgttt taatactata accaccagaa
    13561 tcatacgtta tacctttacc aactaaggca attggtgctt catctttgtc tttgccatta
    13621 tatgtgatgg ttactaatct cggtttatgc ttactacctt tacctactgc ttgtaaaagt
    13681 ccgaatcctt cagaaactaa agtgtcataa tctttaacat ctactttgac ctttgtattt
    13741 ttaaaatgat taacaatatc ttctgcaaat gtttgtggtg ttaatacatt cggtggcata
    13801 ttactaaagt ctcttgccaa attaatggat tggccaatac tgataccctc atgaataaaa
    13861 tctaattcaa ttaatgattc actaataagg tttaaattcg tcttaaacgg tgccttctta
    13921 cttgatttat aatgatcgaa ttcatatgtt gcacgctcac tttgaatacc gcatgccatt
    13981 aatacatctg ataactgatc atattttgaa ataaatgaat ccataagtaa atacgtatct
    14041 tcaatgtgtt ctgactttat gtattggaaa agatgtcccc atattttcaa catatcttga
    14101 taactacgtg tctttaagtt tcctaaacca actgtaatta aacgatatgt ttgatcttgt
    14161 acatcaaatg cagttgtata aatcttccca actttactac caataatatg ttgatgtttt
    14221 agtctttcaa gtgattctgt aatatcgata tgattaaaac taatgcgctc taactgattt
    14281 aaatgttctg gtataccaat aatcaatgta tttatttcgt tgcttagtgt gttatttaat
    14341 ttaaaattca taatgtacct ccttagattt gatatgttac tcacttcaaa ttgtacaaca
    14401 ataaagccct tcagtgacac tgaaggactt tataaatgaa taatttaatt ttatgtgttt
    14461 agctgaaact ttatttaaag cctagaattt accttttttg aatgctaaac cgataccacc
    14521 gattttgaat accgcacgtg tatcaatcac ttttttcatg aatgctgctt ttttaccagc
    14581 gataggttta ccaaatacca tacctacacc gtcatgtgaa cctaaagaac aaacagttcc
    14641 acgatcaacg tattcgaatt cttcagttga ttcaccgttt aagatgcgtt taatgttttt
    14701 agcaacactt tcaccttgtt gcattgcaat ttgtgctgta gttggtaatg gacgttcttc
    14761 tccagctggg ataaacgctg aacagtcacc aataacaaaa atgttgtcgt aaccattgat
    14821 tgttaaatct tgctttgtaa cgatacgtcc acgtttaacg ccttcaaatg attcttccat
    14881 taatttacta ccacgtacac cagctgccca tactgaagta cctgcattta attgttgttt
    14941 ttcaccatct acttcaacta caaaaccttt ttcgttacaa gcaacgattg gtgtagcaat
    15001 tttaaattca acaccgcggt cttctaagta gctaactgcg tggttaacta attcttctga
    15061 gaacattggt aacattttag gtgctgcttc aacacaagtg attttaactt tattttgatc
    15121 cacaccatat ttgctacata attcaggaat tctgtctgtt aattcaccta agaattcaac
    15181 accagtgaat ccagcaccac caactaagat agataaatcg ttatcatctt tttcttttga
    15241 tgctgcatag ttagcaaatt tgtcttcgat atgacgtgat aattcacgtg ctgtgataac
    15301 attttcaatt tggaaagcat gatctttcat accttcgatg ccgaatgttt cactaacgaa
    15361 acctaatgct actactaaaa tatcaaagtc ataaatacct tgatttgttt ctaccttttt
    15421 agcatcacgg tcaatttttg ttacttctgc ttgaacaaag ttcactttgt ctttcttcaa
    15481 gacactttcc acaggatata atacatcttc atagtttagt gtacctgctg atgcttcatg
    15541 taaccatgtt gcttcatagt gatattcatt tttattaata agcgtaattt ctgcttcttc
    15601 tgttgatatc gctttttgca atttagttac agtttgtaaa cctgcataac cagcaccaag
    15661 tacaagtact tttttacgat cttgagccat ttaatttcac ctaagctttc atattttttt
    15721 aaccaaatgc tgataattac tagtaacaac tggtcattac tacttttacc acaatgtata
    15781 ctagtttatc atcattttgt tatttaagtc ttttaaatta tgtaaaaacc tgtgatgttc
    15841 acaaaaaaga catttttaat ccaatactaa ttctatagct tttatgttat attttcaagc
    15901 aagattcagt cagattgtga aaaaattaat taattttttc gtagcctgat ttcgattaaa
    15961 aatgtatccg ctatcacgat catttaaaat actatttata gtcagattct taattttcaa
    16021 taagcttttt aataaaacaa aatatcttta cgcaaaaata ggacataatc aaatatgtcc
    16081 ttaatcgtta ttgatatcaa tttgtatccc aaaaattggg ttaaccatta aaaagttatt
    16141 ttattagcaa ttttcaggat tacctgcaac ttttgcagtt ctaaatgaac taccacagcc
    16201 acatgaagca attgcattag gattgtcgat ttggaaaccg ccacccatta atgattgctt
    16261 aaaatcaata gtcgtaccat ttaatacggg tgcatctttt ttgtctacta atacttttaa
    16321 tccaaagtat tctaagactt catcattttc accaggcgct tcttctgcac ccataccgta
    16381 tgttaaacca gtgcacccgc caccattcac tttaattttt aaatagccat ctggcatttc
    16441 atttgcttta agcatatctt ttacttcgta agcagctgct tctgttaata taactgttgg
    16501 catgataact cctccttaaa aaatccaagt ttcttttata tgtgcatata tattttgtaa
    16561 taattcttcc ggcgaatcac cttcaacaat atcaccattt actaaagcat acaacccggc
    16621 tgaacatata ccacaatgtg tcaggcaacc atactctaac acatcgacat ctgggtcatt
    16681 ttccagttga ttaaaaacat aatctccacc ttttgccatg ttagagagac aaaattctac
    16741 gatcggattc atacttcacc ttcttatttc atttgttaca atattatagc attttaaaac
    16801 tggtatttta acatgatgtg ctcaattagc aacaactgat gtttcttatc ccagttatgt
    16861 aatagtgcct tagttagtac aaatatttat ttgtagtcat tttcaaaaaa atatataata
    16921 agcatataaa tacatattta cttttttagg ggttattgat gaatcaaaga cacttgcatt
    16981 ttataactgt tcattaacaa atatatctat gcactttgat cttttcaaaa ttaaagtaaa
    17041 tattaacata taaaaggggt acttcattat gaaaaactta gttttgttag gcggcggata
    17101 tggtaatatg cgtatcatgt cacgcatttt aactacttct ttaccacaag attatacagt
    17161 cacattagtt gatcgtatgc catttcatgg attgaaacca gaattttatg ctttagctgc
    17221 gggcacgaaa tcagataaag atgttcgtat gaaattccct aatcatccac aagtgaatac
    17281 agtttatggt gaaattaacg acatagattt agatgctcaa attgtctcag tcggtaattc
    17341 taaaattgat tatgatgagc taatcattgg tttaggatgt gaagataaat atcataacgt
    17401 tccaggagcc gaagaatata cacatagtat tcaaacactc tcaaaggctc gggatacttt
    17461 ccatagtatt agtgaactac cagaaggtgc taaagtcggt atcgttggtg ctggattaag
    17521 cggcatagaa cttgccagcg aattaagaga aagtagatca gacttggaaa tatatcttta
    17581 tgaccgtggg ccgcgaattt taagaaattt tccagaaaaa ttaagtaagt atgttgcgaa
    17641 atggttcgcc aaaaataatg ttaccgttgt tccaaattca aatattaata aagttgaacc
    17701 tggtaaaata tataactgtg atgaacctaa agatattgat ttagttgtat ggacagcagg
    17761 aattcaacct gttgaagttg ttcgtaactt gccgattgat ataaatagta atggacgcgt
    17821 gatagttaac cagtatcatc aagtaccaac atatcgtaac gtctatgtag ttggtgattg
    17881 tgctgattta ccacatgcgc caagtgctca gttagccgaa gttcaaggtg atcaaattgc
    17941 cgatgtgctt aaaaagcaat ggctaaatga accattacct gacaaaatgc cggaactaaa
    18001 ggtacaaggt atcgttgggt cattaggaga taagcaaggt tttgcatata tcatggaccg
    18061 cactgtaaca ggacgactcg cttctatttt aaaatccggc gtactttggt tatataaata
    18121 tcacaatggt taaaattaaa aagcaatgtc agttcacact attactgaca ttgcttttag
    18181 ttatgtgtat attattcatt cacaagcttt tggtctataa atcgagtgat ttgctttgtt
    18241 tgtatataac catctgctac atattcatcg ttcattgtaa ttaatggata aaatagttca
    18301 tcttgttcta ttctttcaat aaattgtaaa tcatgatctg ttaagttgtc attatctttt
    18361 gtaatatcta tatacgtata tttaaatgat atatttgggt attttctttt taatagcggc
    18421 tgtagccagt cataaatatc tttcgatgtt ggcgcattaa cgcaacttgc acatataaca
    18481 tctgccccat aaactaccac actcacgtgc tccatatatt tatcccccat tggtttgata
    18541 gatttttatt acactatcta ttataatata ctaataaaga ttatcaaatt catctctcga
    18601 aaggagactt gcctgatgcc tactgaagat acaacgatgt ttgatcaagt agcagaagtt
    18661 attgaacgtc ttcgtccatt tttattacgt gatggtggcg actgctcatt gattgacgtg
    18721 gaagacggta ttgttaaatt acaattacat ggtgcatgtg gtacatgccc aagttctaca
    18781 atcactctta aagctggtat tgagcgtgca ttacacgaag aagtgcctgg tgtaatagaa
    18841 gtagaacaag tattctaata taaagatatt aagttaacag aacatattat gaataacaac
    18901 aaagacagga atactgaatt atttataatc agttattacc tgtctttttt cgctgtcaaa
    18961 ttggactgat gggaaagtta ttttatacta tttatctgat taattatttt attattgcca
    19021 catatccaag cacatattac tattctattc tatgaaattt aaaatataaa aatagcacat
    19081 acgctcaatc aaaatcgttg agttatgtgc tatttgtatt ttaattttta ggtttatcta
    19141 cttcaggttg tggttcacct ttcatatgtt tcgcaatttg ctcatccata taaacccaac
    19201 ctttccaacc gatgtgtacg gcatcactga taacatattt ttcatattct ttatcagtca
    19261 tatcgtaaat tttaccacca ttatctacaa cagtagaatg gattttttta taaactgctt
    19321 gacgacgttc tttatcgata ccaatgtggt cataccatac accgtttgat ggaatactta
    19381 catattgaac atctgcacca gcagcacgca ttgtttttac aagtaattct aaatcttgga
    19441 attctggaga attaacattg aattcgtagt cacgtctaac tttacgctta ctttcttgaa
    19501 ttaatttcca gtattgatct ctaataccaa atttattcga tgtagtatca gctttaccaa
    19561 tttcaactgc tttttgtttc atctcatccc aagaagcgtc tggttttgta gcaggtttaa
    19621 catgttctaa tggagattta tccattgcaa acaatgattt aatcgcttct attttaatca
    19681 attgattctc tttaaaacca gaaatataac tatctttagt ttctttaggg tttttagcat
    19741 aagatttcaa gtattcttta ttgtgtacat gtggaaactg taataaacgt tgtgcataac
    19801 gacgttttaa ttcagtagac atgtttttct gctggaacat ttgattaatt tgagtttgag
    19861 acatacgagc atcaaagttt tgattcgtta aaccatggtt tgtaaaccat tgtggtgaaa
    19921 taataaatgt taatttctta ccttttaatt tatcatactg tgatgcaagt tcaactgcgt
    19981 taattaagtc tgtagaacca ccagcaccta ataagaatgc ttttttgttg gcgttatgct
    20041 tatttaatgc aattgcagga ttaaatgggt catctttacc taattcacta gagccgtata
    20101 taggataata cttgtttgat tcgtataact tatcttgaat gagtgtgcct tttagtactt
    20161 gatctgtcaa tgaagttcta ttatcttcta cagtcttttc atttactaat cctgtaaacc
    20221 aactagcagg taataataga aagacaatga ataccgctcc actaattaaa atgggtaaaa
    20281 aaggttttaa tttcatcgta actcttctaa tgcttcaacg attttatttg gtgttgccca
    20341 ctcatctcta tcaaagtcca taatagatac ttcgatatca agtttatttt gaatctctaa
    20401 taataatcca actgtttgga aagaatcaat aataccttct tcaaaaattt ctacgtctgg
    20461 attttctttt acaatatcat tttctgctac ttctgctaat aaatttaata cttgttctct
    20521 aaattccata attaaattct cctttattat ataagtttac ctgagaagat taaaaagcca
    20581 aatgttacaa agtggaatgt aatcacaatg ctaagtgctg ttgtgaaacc attttgccaa
    20641 cgtggcggat gtttcttacg ccaacgttca taatagccat aacctataaa caatgctgta
    20701 tggtataaac cataaacaat gtaatacact tcgataccat gccaaattcc cattatgaag
    20761 aagttgatta agaatgccac gttagacatt gcaaattgac tcttcaataa ttttttacga
    20821 gacatgtaga ataaagatct catgtaaata caatctctga accagaatga taatgtcata
    20881 tgccatctat tccagaaatc tttaatattt ttcgctttga aaggtttatc gaagtttggt
    20941 ggtgttttaa taccgaataa ataactaaat gctatcgcaa ataaactata acctgcaaag
    21001 tcaaagaata aatataagct gtatgcgtac atatataacc acaaattgac aaagccatgt
    21061 aagtctaatt gtaacggcat gattgcatat gtgttaataa agtaagcaac aatatattta
    21121 tacaagaaac caagcataat catgtgaatt gcttttaata ctaattcacg atattcattg
    21181 cctgttggta cttttttatc gtctttaacg aaacgtttgt aacgatcgat tggtccagat
    21241 gaaatcgttg ggaagaatga aataaattga attaatttcc atactttaat ttctttgata
    21301 gaaccatcac gaatttccat aattaactgc acacttttga atgtaacata agaaattcct
    21361 aagaaaccaa caaattcaat taatttactt tcatggaagt gaatctgatg tccacctaac
    21421 catgtacttt gtaacacttt cacaagtgct aatggcaata ttgataaaac cattacagtt
    21481 acaaattttg aaaatgaatt attttttggt tttgaatgat aataaaacat tatcaataaa
    21541 acttgccata cgacgtaaat aataaaacta attaattgaa cacttaaata cttttggtca
    21601 aacagattat gtttatcaga agaaaagatt aacacaatca taaatgctgt aactacgcca
    21661 ttataaatgt aacttcgctt acctaaaaat ccaagtataa tgactggtaa taatgcaatt
    21721 aaagcaatta agaagaatgt aaaatcacca tatggaatca tccgttaatt acctctgcaa
    21781 ttttctttct gtcaatttta ccatttgaag tcaatggcaa ttgttccatc cattcaaact
    21841 ttctaggaat catatactct ggtaagcgtg atttcaagtc atttttaata tttttagtca
    21901 tttctgcatt gtccgtaact tcagtcgttg gcacaattgc accaattaaa tgaataactt
    21961 tatcattttt atatacaggt acaacaatcg cttcttttac gaactcagac tggcgtaatt
    22021 gtgtttcaat ttcttctaat tccattctgt agccattcaa tttgatttgg aaatcaatac
    22081 gaccttgaat gaaccattga ccattttcaa acttcgcttt atcaccagtg tgatatgtac
    22141 gaataccgtc atcgaaatta aatacttcag ctgttttttg gtcatttttt aagtatccta
    22201 aacttacact ttgaccttcg ataacaagtt caccttcatc tgtagtagat aatcttgcgc
    22261 ctggtctttc aacgccaaca ggtaatgtcg gatattgatc taagatttct tgtgtaattt
    22321 gaatacttgt aactgctacc gtagcttcag ttggaccata tgtgttgtaa atcgtcgcac
    22381 ttgggaaacg gtttactaac gcttttgctg ctctgtgagg tagaatttca ccacagaaga
    22441 agaattcgtt aagactacca tattgttctt cattaagcgt tggtaataat aaacacattt
    22501 ccataaatga tggtgttgat acccaaatgt taatcggtgt tgctgttagc atttcattta
    22561 ataatttagg tttattaatc atgtttttat ctacaagatt taatgtaccg cctgatgcta
    22621 aacatggata aatagccatt acagataaat caaatgaaaa tggcgcttgg ttaagccatt
    22681 gttgttcatt tcctgattta ttaagttcta acatccactc agtaaattga actaaacttg
    22741 catattcaat ttgaacacct ttaggttccc cagtagaacc agatgtaaag attgtgtata
    22801 ctgtgtcgtt atctttaatc tgactatcaa aaattactgg gtcttgagat gttttaatat
    22861 cttctattgt aaatacttcg ccttctaaac tttcaaatga ttcatcagtc gtattaaata
    22921 caaactctgg ttgaaccttg ttaataatca ttttaatacg gtcttcagga attgaagtgt
    22981 ctacaggtac atatccacat cctgctttaa tggcaccaat catcccaaca atcatatatg
    23041 gtgacatgtg accgaataaa atcatcggtt tcttactacc ttgtaatcga tgtgctaatt
    23101 tactagactc atccattaac tgttgataag ttaattcatc agttgtgtgt ctaacagcaa
    23161 tgctttgtgg atttgcatcc gcaaacgctt gcagcttgtt aataatatct gtcatattaa
    23221 gtctccctca ttagaactca ttataaatga agttattgtg tgtgtcgcca ctgccataaa
    23281 ttaaatataa agtaataaat attgccaaat acaatagtgt taataaatat ggtttgaatg
    23341 cttcaacata tttattaggt ggctgtttac ttttagattt catattgcac ctcttaaagt
    23401 tctt
//
LOCUS       DAKTGS010000028        23286 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.028, whole genome shotgun sequence.
ACCESSION   DAKTGS010000028 DAKTGS010000000
VERSION     DAKTGS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.028"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..863
                     /locus_tag="O0286_000940"
     CDS             <1..863
                     /locus_tag="O0286_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0169326.1"
                     /translation="ACGNQGEKNNKAETKSYKMDDGKTVDIPKDPKRIAVVAPTYAGG
                     LKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKEKPDLIIVYSTDKDIKK
                     YQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWEETTAKDGKEIKKAIGQ
                     DATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQKLTAKAGWAEVKQEEIE
                     KYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAGTYWYNDPYTLDFMRKD
                     LKEKLIKAAK"
     gene            1262..2203
                     /locus_tag="O0286_000941"
     CDS             1262..2203
                     /locus_tag="O0286_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0169327.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            2324..2878
                     /locus_tag="O0286_000942"
     CDS             2324..2878
                     /locus_tag="O0286_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCY0169328.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            2950..3720
                     /locus_tag="O0286_000943"
     CDS             2950..3720
                     /locus_tag="O0286_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0169329.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(3892..4689)
                     /gene="fdhD"
                     /locus_tag="O0286_000944"
     CDS             complement(3892..4689)
                     /gene="fdhD"
                     /locus_tag="O0286_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCY0169330.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            4979..5761
                     /gene="modA"
                     /locus_tag="O0286_000945"
     CDS             4979..5761
                     /gene="modA"
                     /locus_tag="O0286_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0169331.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            5775..6446
                     /gene="modB"
                     /locus_tag="O0286_000946"
     CDS             5775..6446
                     /gene="modB"
                     /locus_tag="O0286_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCY0169332.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            6447..7052
                     /locus_tag="O0286_000947"
     CDS             6447..7052
                     /locus_tag="O0286_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0169333.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            7222..8226
                     /locus_tag="O0286_000948"
     CDS             7222..8226
                     /locus_tag="O0286_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCY0169334.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            8257..8763
                     /locus_tag="O0286_000949"
     CDS             8257..8763
                     /locus_tag="O0286_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCY0169335.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(8765..9259)
                     /gene="moaC"
                     /locus_tag="O0286_000950"
     CDS             complement(8765..9259)
                     /gene="moaC"
                     /locus_tag="O0286_000950"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCY0169336.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            9322..10581
                     /locus_tag="O0286_000951"
     CDS             9322..10581
                     /locus_tag="O0286_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCY0169337.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            10578..11063
                     /gene="mobB"
                     /locus_tag="O0286_000952"
     CDS             10578..11063
                     /gene="mobB"
                     /locus_tag="O0286_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCY0169338.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            11077..11523
                     /locus_tag="O0286_000953"
     CDS             11077..11523
                     /locus_tag="O0286_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCY0169339.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            11529..11762
                     /gene="moaD"
                     /locus_tag="O0286_000954"
     CDS             11529..11762
                     /gene="moaD"
                     /locus_tag="O0286_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCY0169340.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            11769..12368
                     /gene="mobA"
                     /locus_tag="O0286_000955"
     CDS             11769..12368
                     /gene="mobA"
                     /locus_tag="O0286_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCY0169341.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            12388..13410
                     /gene="moaA"
                     /locus_tag="O0286_000956"
     CDS             12388..13410
                     /gene="moaA"
                     /locus_tag="O0286_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCY0169342.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(13805..13975)
                     /locus_tag="O0286_000957"
     CDS             complement(13805..13975)
                     /locus_tag="O0286_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169343.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            14101..14451
                     /gene="sarV"
                     /locus_tag="O0286_000958"
     CDS             14101..14451
                     /gene="sarV"
                     /locus_tag="O0286_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCY0169344.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(14553..15764)
                     /locus_tag="O0286_000959"
     CDS             complement(14553..15764)
                     /locus_tag="O0286_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0169345.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(15757..16197)
                     /locus_tag="O0286_000960"
     CDS             complement(15757..16197)
                     /locus_tag="O0286_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCY0169346.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            16359..17123
                     /locus_tag="O0286_000961"
     CDS             16359..17123
                     /locus_tag="O0286_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0169347.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(17115..17366)
                     /locus_tag="O0286_000962"
     CDS             complement(17115..17366)
                     /locus_tag="O0286_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169348.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            17755..19020
                     /gene="femX"
                     /locus_tag="O0286_000963"
                     /gene_synonym="fmhB"
     CDS             17755..19020
                     /gene="femX"
                     /locus_tag="O0286_000963"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCY0169349.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            19137..22304
                     /locus_tag="O0286_000964"
     CDS             19137..22304
                     /locus_tag="O0286_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCY0169350.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(22491..22808)
                     /gene="mspA"
                     /locus_tag="O0286_000965"
     CDS             complement(22491..22808)
                     /gene="mspA"
                     /locus_tag="O0286_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCY0169351.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(23053..23217)
                     /locus_tag="O0286_000966"
     CDS             complement(23053..23217)
                     /locus_tag="O0286_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169352.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
BASE COUNT         8452 a         3241 c         4436 g         7157 t
ORIGIN      
        1 ctgcgtgtgg gaaccaaggt gaaaaaaata acaaagctga aactaaatct tataaaatgg
       61 acgatggcaa aacggtagat attccgaaag accctaaacg cattgcagta gttgcgccaa
      121 catatgctgg tggacttaaa aaattaggtg caaacattgt agctgtaaat caacaagtcg
      181 atcaaagcaa agtattaaaa gataaattta aaggtgttac aaaaattggt gatggcgatg
      241 tagaaaaagt tgctaaagaa aagccagatt taattattgt atactctact gacaaagata
      301 ttaaaaaata tcaaaaagta gcaccaacag tagttgttga ctataataag cataaatatt
      361 tagaacaaca agaaatgtta gggaaaattg ttggtaaaga agataaagta aaagcttgga
      421 agaaagattg ggaagaaaca actgctaaag acggtaaaga aattaaaaaa gcaattggac
      481 aagatgcaac agtgtcattg tttgatgaat ttgataaaaa attatacact tacggcgata
      541 actggggtcg tggtggagaa gtattatatc aagcatttgg tttgaaaatg caaccagaac
      601 aacaaaagtt aactgcaaaa gcaggttggg ctgaagtgaa acaagaagaa attgaaaaat
      661 atgctggtga ttacattgtg agtacaagtg aaggtaaacc tacaccagga tacgaatcaa
      721 caaacatgtg gaagaatttg aaagctacta aagaaggaca tattgttaaa gttgatgctg
      781 gtacatactg gtacaacgat ccttatacat tagatttcat gcgtaaagat ttaaaagaaa
      841 aattaattaa agctgcaaaa taattcagct atataagtta gtgaaatgag agtctgaaac
      901 atatcaatct tttgatattg tattaggctc ttatttttat agctagaaag ttagatattt
      961 gtattttttt aaataataag tgccgttgtt atcgttcaat ttaattaatg atagattagt
     1021 attattatag ctaaagtagt atacctgaga aaatagctca atgtatctct ttattaataa
     1081 gttatatcat aattatttta gtgcatactt tatggaaggg atatcaggga atggctttca
     1141 attaaagaag aggtttaaaa ggattacaac agaatgttat gattttgtag aaagatatat
     1201 aacaacgttt tataaaaaca taatattgtt aatggaaaat gaaatgtaag ggggatttcg
     1261 agtgactaag aaagtttatt ttaaccacga tggtggtgta gatgatttag tatctctatt
     1321 tttattatta caaatggaaa acgttcaatt gataggggtc agtacaattg gtgctgattg
     1381 ttatttagag ccatctttga gcgcatcagt aaaaattatt aatcgttttt caaatgaaga
     1441 tattcaagtt gcgccatcat atgaacgagg aaaaaatcca tttcctaaag aatggcgtat
     1501 gcatgccttt tttatggacg cattgccaat tttaaatgag ccagtcaaac atgttgcttc
     1561 aaatgtgagc gacaaagaag cctttgaaga cattattcaa actttaaaga gacaatcaga
     1621 aaaagtaaca ttattattta caggcccgct tacagattta gcaaaagcac tacaaaaaga
     1681 ttcatctatc gttcagtata tagaaaaatt agtttggatg ggtggcacct ttttaccaaa
     1741 aggaaatgtt gaagaacctg agcatgatgg ttctgcagaa tggaatgcat attgggatcc
     1801 agaagcggtt aaaattgttt ttgatagcga tatagagatt gatatggttg ctttagaaag
     1861 tacgaatcaa gtaccgctaa cgttagatgt tagacaaaga tgggcgaatg aacgccaata
     1921 tacgggcatt gattttttag gagtaagtta tgcagcggta ccaccattaa cacactttat
     1981 aacaaattct acttactttt tatgggatgt tttaacgact gcttatattg gtaacaagga
     2041 cttggttcat tcaattgaga aaaaagtcga tgtaataagt tatggaccaa gtcaaggtaa
     2101 gacatttgag tgtaaagatg ggcgcaaaat taatgtcata aatcatgtag ataacaacgc
     2161 attttttgat tatataactg cacttgctaa aaaagtaaat taacagctgt gtagaataat
     2221 taaggtttta atttatatag aacaacttat tgtaaacttt tcatttctta aagtttacaa
     2281 tggtgctata ataatggtca tgaaatacga aaggaagtaa aaaatgacaa caaaacagtt
     2341 agtatataca gctttaatga cagcgattat cgctatttta ggattggtac cggtaattcc
     2401 actaccattt tcttcagtac caattgtact tcaaaacatt ggtattttct tagcaggtgt
     2461 gattttagga cgtaaatatg gcacattaag tgttatcgtc tttttattat tagtagttgc
     2521 tggcttgcca ttgttatcag gtggtcgcgg tggcatcggt gtattcgcag gtccttcagc
     2581 agggttttta ctattatatc cagttgtagc attcatgatt ggggcgattc gagatagatt
     2641 catcaatgaa attaatttct ggattttatt cgttggtatt ttagtttttg gtgttatagc
     2701 attagatgtt attggtacat tgattatggg catgattatt aacataccat ttacgaaagc
     2761 tatttcaatt tcattagctt atttgcctgg tgatatatta aaagcaattg tagcaagttt
     2821 gattggtaca gctttactta atcactcgca gtttcgtcaa attatgggaa taaaataatc
     2881 atatttaaga tagtaaagta attgaataag ttgctttgaa atttataaaa gtgaaaggag
     2941 taggtgtcaa tggctagtat aagtatgtca gatatatatt gtaacggcac tatatttgaa
     3001 aatgacgacg agcagttgat ttatttaacg ccttcttttc cacaacgata cacaagtaac
     3061 acatggatat ataaaaagac gcctacccaa gagcgatggc tgaaagactt agaacgtcaa
     3121 catcaattac atacaaatca aggttcaaat cattatgcgt ttagtttccc ggaaaatgaa
     3181 caacttgata atcattggat ggctatgttt aaagatatga attttgaact aggtattatg
     3241 gaattgtatg ccatagaaag tgatgcgctt gccaatttgc cgcgtaactc tgacgttgaa
     3301 attgccatcg ttgacgagtc gcatatagat gcctatttaa aagttgcata tcagtttagt
     3361 ttgccatttg gaaaagacta tgcagatgca catgaagaaa tggtaaggga acattatcaa
     3421 aaagatgtga ttaaacgctt agtagcttat ttaaataatg aacctattgg cgttgtagat
     3481 gtcattgaaa gtgaaaatta cattgaatta gatggatttg gtgtattaga acaatttcgg
     3541 caccaaggaa ttggatctac aattcaatcg ttgataggtg aatacgccat atcaaaaaat
     3601 cacaaaccaa tcatattagt tgcagatggt gaagatacag caaaagatat gtatgcaaag
     3661 caaggttatg tctatcaatc gttttgttat caaatattaa aagaagatat tggaaattaa
     3721 cgattgatga taggtttgta ttagaataaa aaagcagtaa gctggtttcg agtagaaatc
     3781 aacttactgc tttttaaatt gttttgagct acttatactt ataaaaatag tgcgtttaaa
     3841 ttgttgattc atgtagaata tcgttcatta tgacacacta taatgaatat gttattgttc
     3901 agaatcaatg atacgttctg gatgactgta tatattaaag ccaccatttc gaataaatcc
     3961 aactgccgta atatttaggt cattagctaa ggttacagca agcgttgtcg gagctgattt
     4021 agataaaatg acgccaacac caatttttgc ggctttaatt aaaatttctg atgaaatacg
     4081 tccactaaaa attaatactt tatctcggac agtaatatgt cgctgaatac aaaatccata
     4141 taatttatct agagcgttat gtctaccaat gtcttgtcga tgtacaaaaa atgtcaaacc
     4201 atcgcttata gcagcattat gtaagccacc tgtttcttgg taaatatgac ttgcactttg
     4261 taatcgagtc atcatgttaa taatttgcat tggagttaaa gtgattttag acatagatgt
     4321 tttagcgata gcagcatcat tttgaaaata aaactcacga ctctttccgc aacaagatgc
     4381 aatcattcgt tttgtggaat attgaaagcg atcgcctaaa tctttattaa gttcaacatg
     4441 ggcaaaacct ttactatcat caatcagtac agattttaat tcatctcgct ttaaaatggc
     4501 accttccgaa gccagaaatc caatgactaa ctcctcaagg tttgttggac tgcatataac
     4561 agtcgcaaat tcttcaccat tcaccataat tgtaagtgga aattctgtca catattgatc
     4621 tgttgtattg aataattttc catcttcata tctaacaatt ggttgaccta aagatacatc
     4681 tttgttcatt atctaacccc tttaattagc ttaaacttta ttttaaagca atttgcttaa
     4741 aattttaaca tatttgctta agtttgaaat ttgattgata aaaattaata gcgagcaatc
     4801 tgtttgattt aaattgaatt cgagaatata catactaggg catcaattaa taaatatcaa
     4861 tcttatgcaa atttgacaat tgtttgaatc aatatataaa caggcaacgg ttcttttcaa
     4921 atataatagt aagtgtataa tgaaaatgta aatattatta aaaatggggg ttcactcaat
     4981 gaaattgaaa cgtttatttg ctgttgtgat tgcaatgctt ttagtattag ctggttgctc
     5041 taattctaac gataataatg aaagtaaaaa agatgacgca gacaatggta agaaacaaga
     5101 gattcaagtt gcagcggcag caagtttaac agatgtaacc aagaaattag cttcagaatt
     5161 taaaaaagag cataaaaatg ctgatattaa atttaactat ggtggatcag gggcattaag
     5221 aaaacaaatt gaatcaggcg cacctgttga cgtatttatg tctgcaaata ctaaagatgt
     5281 agatgcatta aaagacaaga ataaagcgca tgatacatat aaatatgcga aaaatagtct
     5341 agtattaatt ggtgataaag attcaaatta cacttcagta aaagacttaa aagacaatga
     5401 taaattagca ttaggtgaag tgaaaactgt accagcagga aaatatgcga aacagtattt
     5461 agataacaat aacttattta aagaagtcga aagtaaaatc gtttatgcta aagatgtaaa
     5521 acaagtatta aattatgttg aaaagggtaa tgcgaaacaa ggttttgtgt ataaaactga
     5581 cttatataaa caaaataaaa aaattgatac tgtaaaagta attaaagaag tagaacttaa
     5641 gaagccaatc acatacgaag ctggtgctac atcagatagt aaattagcaa aagagtggat
     5701 ggaattctta aaatcagata aagctaaaga aatactaaaa gaataccact ttgcagcata
     5761 aggagttgta atccatgcct gacttaacac ctttttggat atcaatacga gttgctgtaa
     5821 tcagtacgat tattgtaacg gttttaggta tttttatatc taaatggttg tatcgtcgta
     5881 agggttcgtg ggttaaatta ttggaaagtt tattgatatt acctattgtt ttgccgccaa
     5941 cggtattagg ttttattcta ttaatcatct tctcgccaag aggaccaatc ggtcaattct
     6001 ttgcgaatgt actacattta cctgtagtgt tcactttgac aggtgctgtg atagcatctg
     6061 tcattgttag ttttccacta atgtatcaac atactgtgca aggcttcaga ggtatagaca
     6121 cgaaaatgat taatacagct agaacgatgg gagcaagtga aacgaaaatt ttcctcaaat
     6181 taattttacc attagctaaa cgctctattt tagcaggtat aatgatgagt tttgctcgtg
     6241 cattaggtga gtttggtgct acattaatgg ttgcaggata tattccaaat aaaacgaata
     6301 cactaccttt agaaatatac ttcttagtgg aacaaggtag agaaaatgaa gcgtggttat
     6361 gggtattagt gctagtcgca ttctctattg tggttatatc tacaattaat ttattgaata
     6421 aagataaata taaggaggtc gactagatgc ttaaaatcaa tgtgaaatat caattaaaga
     6481 acactttaat tcgcatcaat atagatgata ctgaaccaaa aatttatgca gttcgtggtc
     6541 catctggcat tggtaaaact actgttttaa atatgattgc cggattacgt aaagcagatg
     6601 aagctattat cgaagtgaat gggcaattac ttactgatac ggcaaaaaac gtgaatgtta
     6661 aaattcaaca acgacgtatt ggatatctgt ttcaagacta ccaattgttt cctaatatga
     6721 cggtctataa aaatattact tttatggctg aaccatctga acacatcgat caattaattc
     6781 aaactttaaa cattgatcat ttgatgaaac aatatcctat gacattgtca ggtggagagg
     6841 cacaacgtgt agcacttgca cgtgcactta gcacgaaacc agatttaatt ttattagatg
     6901 aacctttttc tagtttggat gatactacaa aagatgagag tattacatta gttaaacgta
     6961 ttttcaacga atggcaaata ccaatcatat ttgtgacaca ttcaaactat gaagcagaac
     7021 aaatggctca tgaaattatt acaattgggt aatcatttat ttgccattaa agagtttaga
     7081 acgtatttaa aattgtagaa gtgaatgctt ctatcagcat tttaatgatg ttttaaactc
     7141 ttttttaggg gcagtttttt tgagagacat tgacgcgcgt catataatga aagtaatgat
     7201 aaaaagaaag gataacttaa tgtgagtcaa gaacgttatt caaggcaaat tttatttaaa
     7261 caaataggtg aaataggtca aagcaaaata aatcaaaaat gtgcgttgat tattggtatg
     7321 ggcgcattag gtacacatgt ggccgaagga cttgttagag caggcattgc caaactaatc
     7381 attgttgata gagattatat tgaatttagt aatttacaaa gacaaacatt gtttactgaa
     7441 gaagatgctt tgaaaatgat gcctaaggtg gttgcagcta aaaagcattt gctagcgtta
     7501 cgtagtgatg ttgatattga tgattatatt gcccatgtgg attattattt tttggaaaca
     7561 catggacagg acgttgacgt tattattgat gcaaccgata actttgaaac acgacaactg
     7621 attaatgatt ttgcatataa atatcgtata ccttggattt atggtggtgt tgtacagagt
     7681 acatatacag aagctgcatt tatacctggt aaaacacctt gctttaactg tttggtacca
     7741 caattgccag cattaaattt aacatgtgat acagtagggg tcattcaacc tgccgtgacg
     7801 atggcaacaa gtttacaatt aagagatgcg atgaaagtat taacggaaca accaattgac
     7861 acaaaaataa cttatggcga tatttgggaa ggtagtcatt attcatttgg tttcagtaaa
     7921 atgcaacgtt cagactgtac aacttgtgga gatgtaccaa gttatccgta tttaaacaag
     7981 aatgaacaac gttatgcaac attgtgtggt agagacactg tacagtatga aaatgcatca
     8041 attacacacg acattcttgt tcaattttta aaacaacatc agttaaatta tcgcagtaat
     8101 tcgtatatgg ttatgtttga atttaaagga caccgcattg ttgcttttaa aggtggaagg
     8161 tttttaatac atggcatgac acgcacatca gatgccacac atctaatgaa tttattgttt
     8221 ggataaaaaa agataagaca aaaggagtgt aatattatgg gcgaacatca aaacgttaaa
     8281 ttgaatcgta cagttaaagc agccgtacta acggtatcag atactagaga ctttgataca
     8341 gataaaggtg gtcaatgcgt gcgccaacta ttacaagcag atgacgttga agtgagtgac
     8401 gcacattata caattgtgaa agatgaaaaa gtagccatca cgacgcaggt gaagaagtgg
     8461 ttagaagaag atattgatgt catcattacg actggtggaa caggtattgc acaacgtgat
     8521 gtgacgattg aagcagtaaa accactttta actaaagaga tagatggctt tggggaattg
     8581 tttagatatt tgagttatgt tgaagatgtt ggcacgcgtg cattattgtc tcgtgctgta
     8641 gcaggtacag ttaataataa attgatattt tcgattccag gatcaacagg cgcagttaaa
     8701 ttagcattag aaaagctcat taaaccagaa ttaaatcatc tgattcatga gcttacaaaa
     8761 taatttattg atttgattgg cgttgaaaat ctccagattt accgccagac ttgctttcaa
     8821 ggtaggtttc gccaataatc atacctttat caactgcttt cgtcatgtcg taaatggtta
     8881 aagccgttgc tgatgcagcg gttaaagctt ccatttcaac accggttttg ccagttgtag
     8941 agacagttgt ttgaatgttt aaagtataaa ggggtgcatt tgtttcatcc cagctgaagt
     9001 gaacatctat gccagtcaat ggtaatggat ggcacatcgg aataagtgtt gatgtatttt
     9061 tggcagccat aataccagcg atttgagcag tgttcaatac attgcctttg gtattggtgt
     9121 tattagaaat ctgcttaaaa atagtttcat ttacagtaat gcttgaatga gcgacagcag
     9181 ttctttttgt aatttgtttg tctgatacat cgaccatttt ggcgtggcct tgttgattaa
     9241 tatgagtaaa ctcagtcatt ttacccctcc tagtgcatct agtatatcat gaaaaaataa
     9301 aagttttgga gatgattttt aatggtagta gaaaaaagaa acccaatccc agttaaagaa
     9361 gcaattcaac gtatcgttaa tcagcagagt tcaatgccgg caattacggt agcacttgaa
     9421 aaaagtctaa atcatatctt agcagaagat attgtagcta cttatgatat accaaggttt
     9481 gataaatcac cttatgatgg ttttgcaatt cgcagtgttg attcacaagg ggcaagtggt
     9541 cagaatcgca ttgagtttaa agtgattgat catattggtg caggttcagt ttctgataaa
     9601 ttagttgggg atcacgaagc ggtgcgtatt atgactggag cacaaatacc taatggcgca
     9661 gatgctgttg ttatgtttga acaaacgatt gaactagaag atacatttac aattcgtaaa
     9721 ccattttcaa aaaatgaaaa tatatcttta aaaggtgaag aaacaaagac aggcgatgtt
     9781 gttctaaaaa aaggacaagt aattaatcca ggggctatcg cggtccttgc aacatatggc
     9841 tatgcagagg ttaaagttat taagcaaccg agtgtcgctg ttattgcaac aggaagcgaa
     9901 ttattagatg ttaatgatgt attagaagat ggaaaaattc gtaactctaa tggcccaatg
     9961 attcgtgcct tagcagaaaa attaggtctt gaagttggta tttacaaaac acaaaaagat
    10021 gatttagata gtggcatcca agtcgttaaa gaagctatgg aaaaacatga tatcgttatt
    10081 acaacgggcg gagtttctgt tggagatttt gactatttac ctgagattta taaggctgta
    10141 aaggcggaag tgttatttaa taaagtagca atgcgtcctg gtagcgtaac aacggttgca
    10201 tttgtagatg gaaagtattt gtttggatta tctggaaatc catcagcttg ttttacagga
    10261 tttgaactat ttgtgaaacc agctgttaaa catatgtgtg gcgcactaga agtcttcccg
    10321 caaataatta aagcaacatt aatggaagat tttaccaagg caaacccatt cacacgattt
    10381 atacgtgcta aagcaacgtt aacaagtgct ggagctactg tagtaccttc aggattcaat
    10441 aaatcaggtg cggttgtagc gattgcacat gctaactgta tggtcatgtt accaggaggg
    10501 tcacgtggtt ttaaagcggg gcatacagta gatattatat tgactgaatc tgacgctgct
    10561 gaagaggaac ttcttttatg attttacaaa ttgtaggtta caaaaagtct ggtaagacaa
    10621 cattgatgag gcatattgtc tctttcttaa agtcacatgg ttatacagtt gctactatta
    10681 aacatcatgg gcatggtaag gaagatattc aattacagga ttcagacgtc gatcacatga
    10741 agcattttga agcgggggca gatcaaagta ttgtacaagg ttttcaatat cagcaaactg
    10801 taacacgtgt agataatcaa aatcttactc aaattattga aaaatctgtt acaattgaca
    10861 ccaatatcgt attagttgaa ggctttaaaa atgctgattt tgaaaaagtc gtagtctatc
    10921 gaaatgaaga agagttgcaa gtattacaac aattgtcgaa tgtttgttat agcattaatg
    10981 taagggagca tgaagatttt acagcatttg agcaatggtt attaaataaa attaaaaatg
    11041 attgtgatac acaattaaca tagaggattg aaatgaatga aacaatttga aatcgtgaca
    11101 gaaccgatac aaacagaaca atatcgtgaa ttcactataa atgaatatca aggtgcagta
    11161 gttgttttta ccggtcatgt tcgcgaatgg actaaaggcg tcaaaacgga atatttagaa
    11221 tatgaagcgt atattccaat ggctgaaaag aaattggcac aaattggaga tgaaataaat
    11281 gaaaaatggc ctggaacgat aacgagtatt gttcatagaa tagggccatt acaaatttca
    11341 gatatcgctg tattaattgc ggtttcttca ccgcatcgta aagatgccta tcgagcaaat
    11401 gaatatgcaa ttgagcgtat aaaagaaatt gttccgattt ggaaaaaaga aatttgggaa
    11461 gatggttcaa aatggcaagg gcatcaaaaa gggaattatg aagaagcgaa gagggaggaa
    11521 taagagagat gaaggtactt tacttcgcag aaattaaaga tatattacaa aaagcacagg
    11581 aagatattgt gcttgaacaa gcattgactg tacaacaatt tgaagattta ttgtttgaac
    11641 gttatccgca aatcaataat aaaaagtttc aagttgctgt aaatgaggaa tttgtacaaa
    11701 aatcggattt cattcaacct aatgatactg ttgcattaat tccaccggtt agtggaggtt
    11761 aagggagcat gaaagcaata attcttgcag gtggtcattc agtgcgattt ggtaagccca
    11821 aagcttttgc ggaagtgaac ggtgagacct tttatagtag agtaattaag acattagaat
    11881 caacaaatat gttcaatgaa attattatta gtacaaatgc gcaattggca acgcaattta
    11941 aatatccaaa tgttgttata gatgatgaga atcataatga taaaggtcca ttagcaggaa
    12001 tttatacaat catgaagcaa catcctgaag aagaattgtt ttttgtcgtt tctgttgata
    12061 caccaatgat tactggtaaa gctgtaagca cgttgtatca gtttttagtt tctcatctta
    12121 ttgaaaatca tttagatgtc gcagctttta aagaagatgg acgttttatt ccaacaattg
    12181 cattttatag tccgaatgca ttaggcgcta taactaaagc actacattct gataattaca
    12241 gttttaaaaa tgtatatcat gaattatcaa cggattattt ggatgtaagg gatgtagatg
    12301 cgccctcata ttggtacaaa aatataaatt atcagcatga tttggacgct ttaattcaaa
    12361 aattgtaagc tgttaggagg tccacaaatg gtagaacaaa taaaagataa actaggacgt
    12421 cccatccgtg acttacggtt atctgtgaca gatcggtgta actttaggtg tgattattgc
    12481 atgcctaaag aggtatttgg agatgatttc gtatttttac ctaaaaatga acttttaacg
    12541 tttgatgaaa tggctagaat cgctaaggta tatgcagaat taggtgtaaa aaaaatacgc
    12601 attacaggtg gagaaccatt gatgcgacgg gatttagatg tacttatagc taaattaaat
    12661 caaatcgatg gtattgaaga tattggtttg actacaaatg gtttgttatt aaaaaagcat
    12721 ggacaaaagt tatatgatgc tgggctgcgc agaattaatg tcagtttgga tgctattgat
    12781 gatacgctat ttcaatcaat caataatcgt aatattaaag cgactacgat tttagaacaa
    12841 attgattacg cgacgtctat tggtttgaat gtaaaagtaa atgttgttat acaaaaaggt
    12901 attaacgatg atcaaatcat accaatgctt gaatatttta aagataaaca tatagagatt
    12961 cgatttatag aatttatgga tgttggtaat gataatggat gggatttcag taaagttgta
    13021 actaaagatg aaatgcttac aatgatagag cagcactttg aaatcgatcc tgtagaacca
    13081 aaatattttg gggaagtagc aaaatattat cgccataagg ataatggtgt tcaatttggt
    13141 ttgattacaa gtgtttcaca atcattttgt tctacatgta cacgcgcaag gctgtcatca
    13201 gatgggaagt tttacggatg tttatttgca actgtcgatg gatttaacgt taaagcgttt
    13261 attcgttctg gcgtgaccga cgaagaatta aaagaacaat ttaaagcttt atggcaaata
    13321 agagatgatc gatattcaga tgagagaact gctcaaacag ttgccaatcg tcaacgtaaa
    13381 aagataaaca tgaattatat tggtggttaa tgtgtaggga ccactacata ttaaatcatt
    13441 agagatgttt taatatttct gtcttactcc ctaaaataca atattattta ttaaagtaaa
    13501 aacggtcata tctatgccag atttaataga aatgatcgtt tttaaagttt ttacaagttg
    13561 gcggggcccc aacacagaag ctgacagaaa gtcagcttac aataatgtgc aagttggcgg
    13621 ggccccaaca tagagaattt caaaaagaaa ttctacagac aatgcaagtt ggggaacggg
    13681 gccccaacac agaaggtgac gaaaagtcag catacaataa tgtgcaagtt ggcggggccc
    13741 caacatagag aatttcaaaa gaaattctac agacaatgca agttggggat caacgaaata
    13801 aattttatga gaatatcatt tctatcccac tcttaagaat cactacataa taaatcttta
    13861 gtggttcttt aacattgatg tcacactcca tgccattgag ttgtaatata tcttttttag
    13921 gtataaatgt tgtcgaataa acaacaagtt gtccaaaaga tataaatcta aacaagatat
    13981 agccagcaat ttaatatttg taatagataa aatgctaagt ttgatatata ataaatttaa
    14041 gtaattgtat aataatatga attacaaaca tctaagaaga aacataggag gcatcatatt
    14101 atgagtaata aagttcaacg ttttatagaa gcagaaaggg agttaagtca gttaaagcac
    14161 tggttaaaaa caacacataa gatttcaatt gaagaatttg tagtcctttt taaagtgtat
    14221 gaagctgaaa agattagcgg taaagaattg agggatacat tacattttga aatgctatgg
    14281 gatacaagta aaatcgatgt gattatccgt aaaatctata aaaaagagct tatttctaaa
    14341 ttgcgttctg aaacggatga aagacaagta ttctatttct atagtacttc tcaaaagaaa
    14401 ttgttagata aaattactaa agaaatagaa gtgttaagcg ttacaaacta aaaacttaaa
    14461 aagcatgcca atcagtacat cataattgcg tcttggggac agacaaatga tgaatagaga
    14521 ttggcatgct ttttattttt gaatataaat atttagttca tggcatttct agttacatga
    14581 cgtccatgaa ttaagaagta aacaagcata gtaatgattg ctaaagcggc cataaagccg
    14641 aagatttcac tatatgaaaa catatgagta aataacccaa ggaatgatgg accgaagccg
    14701 acacctgcat ctagaccaac gtaaaaagta gatgtcgcga taccatattt aatcgggggt
    14761 gagactttta tcgcaataga ttgcattgca gatgataaat ttccataccc taaacctagg
    14821 caagcaccag caagtaatat taaccagctt tgatagcttg aaattaagca tacaaatgaa
    14881 aggaaaagca tgataaatgc tgggtagaca ataatatttt catttttatc atccatcaat
    14941 ctaccagcaa taggtctagt aattaacgat gctatagcat agcaaataaa gaaatagctt
    15001 gctgcagtga ctaggtgtcg ctctaaagca aatgcttgta aataagttag gatggacgca
    15061 taggtaacgc caattaaaag cataattaca gcaacaggaa tggcctcttt tgcaataaat
    15121 tgatgaatac taaatcttgg tttatcaatg acattagttt cagttttgtt atttgttact
    15181 tcgaaatcaa cttttataaa taatgagata atgagtccga gtatgcctaa tatgacacaa
    15241 ataataaaca gtaagtcaat tgcgtatttt gtaataagta acatgcctag aaatgggcca
    15301 atcgctgtac ctaatactaa acttaaggaa aataaactga tgccttcact ttttctatta
    15361 acaggggtaa cgtatgccgc aatagtacct gttgcagttg tcacaactgc agttgcgata
    15421 ccgtttatga gacgtacaaa gattaaaaaa gctaaagatc catcaataaa ataaagtaat
    15481 tgcgtgataa ttaaagcaat taaaccaata aataataatc gtttaggtcc gatttgattt
    15541 acaaatttac ctgtagcaaa tcgaccaatt aaagacccga caataaatag tcctacaact
    15601 aatccggcta atgaatcgga agcgtcgaaa gcttgcttac tatagccagc aataactaca
    15661 agtaacaaat acatgcatag gtatacgata aagttgacta aaaagttaat tgtaaaactt
    15721 ttggtgaaaa taggtgattt atttaatgag ccttgattag tcattgtgtg cctcccttaa
    15781 taatatttca tgaatttgat tcatcacatt tatggtttct tgcatttgat cttgcgaaat
    15841 gtttgttttg gcaactactg cttgttggag cttttgtaca tcaagacaaa caatctcata
    15901 taattcatgc cctttaggtg ttaaagttaa aaacttttgt cgtttatcct ctaaactgtt
    15961 ttctactgtt ataaggtcat tttctattag agcttttaaa gtttttcttg cagtaggctt
    16021 ttcaattgca cgtctatgtg aaatttcaat gagagtagtg ggctgatgtt tagcgatatc
    16081 gcgtaagatt aaccattggc cataatatat attgtgtttt tctaaaatcg gctcggctaa
    16141 ttttaaatag gggcgatata ttgttataaa actattgaaa aattcttgtg atagcatagt
    16201 gacatctcct aagacaaaat agttagctta gctaaccttt ttacaacaat agtaattata
    16261 aaacgggagc aattagaaat caatatataa ttattaagag caaaaataat tatactttgt
    16321 taaaataagc gtaattacat gtaaataggg ggatactaat gatattgaaa tttgatcaca
    16381 tcattcatta tatagatcag ttagatcggt ttagttttcc aggagatgtt ataaaattac
    16441 attcaggtgg gtatcatcat aaatatggaa cattcaataa attaggttat atcaatgaaa
    16501 attatattga gctactagat gtagaaaata atgaaaagtt gaaaaagatg gcaaaaacga
    16561 tagaaggcgg agtcgctttt gctactcaaa ttgttcaaga gaagtatgag caaggcttta
    16621 aaaatatttg tttgcgtaca aatgatatag aggcagttaa aaataaacta caaagtgagc
    16681 aggttgaagt agtagggccg attcaaatgg aaagagatac acataaagat ggtaaggtaa
    16741 agtggcaatt gctttatata atgaatcagg atgatgatga aattaagcca ccatttttta
    16801 ttcaatggga agaaagtgat tccatgcgta ctaaaaaatt gcaaaaatat tttcaaaaac
    16861 aattttcaat tgaaactgtt attgtgaaaa gtaaaaaccg atcacaaaca gtatcgaatt
    16921 ggttgaaatg gtttgatatg gacattgtag aagagaatga ccattacaca gatttgattt
    16981 taaaaaatga tgatatttat tttagaattg aagatggtaa agtttcaaaa tatcattcgg
    17041 ttatcataaa agacgcacaa gcaacttcac catattcaat ttttatcaga ggtgctattt
    17101 atcgctttga accattagta taaatatacg taagtgctat gagcgagaat gcccatatga
    17161 ataatgacaa gcacaatgga aagaatcgtt aatatattat ttaatcgtga tgacttaatt
    17221 aaaatgaaaa agattgataa tataaatgtg aaaaagataa gtataacccg taaactaaag
    17281 taattcacgg tgagaggttg actcaatgtc ataatgattg caacgatgtt cataattata
    17341 aatagactta aaataattgt tctcatatca aacacctcat tgttagatta ttgacattat
    17401 aacaggggta attgtatatg aacattaatg tggttgcttg aggaaaaatt tattcattga
    17461 agtcaagttg gttcatttta gaaatgaata tcgtgttaga tgatgaaagt atattgaagt
    17521 ataggtaact agttgaaaag tattaattgt acgataacat taaatttaac acgaaacata
    17581 gatataaaat gattcacaat taaaatgggt aaatttgaac ttgctaaact attaattgga
    17641 gcatggacat ttcaaaaata agagttcaaa tcttacacaa gctctgaatc gacactataa
    17701 gatacaaact gtataattaa aggtattgtt aaatagaagg agatatcata aatcatggaa
    17761 aagatgcata tcactaatca ggaacatgac gcatttgtta aatcccaccc aaatggagat
    17821 ttattacaat taacgaaatg ggcagaaaca aagaaattaa ctggatggta cgcgcgaaga
    17881 atcgctgtag gtcgtgacgg tgaagttcag ggtgttgcgc agttactttt taaaaaagta
    17941 cctaaattac cttatacgct atgttatatt tcgcgtggtt ttgttgttga ttatagtaat
    18001 aaagaagcgt taaatgcatt gttagacagt gcaaaagaaa ttgctaaagc tgagaaagcg
    18061 tatgcaatta aaatcgatcc tgatgttgaa gttgataaag gtacagatgc tttgcaaaat
    18121 ttgaaagcgc ttggttttaa acataaagga tttaaagaag gtttatcaaa agactacatc
    18181 caaccacgta tgactatgat tacaccaatt gataaaaatg atgatgagtt attaaatagt
    18241 tttgaacgcc gaaatcgttc aaaagtgcgc ttggctttaa agcgaggtac gacagtagaa
    18301 cgatctgata gagaaggttt aaaaacattt gctgagttaa tgaaaatcac tggggaacgc
    18361 gatggcttct taacgcgtga tattagttac tttgaaaata tttatgatgc gttgcatgaa
    18421 gatggagatg ctgaactatt tttagtaaag ttggatccaa aagaaaatat agcgaaagta
    18481 aatcaagaat tgaatgaact tcatgccgaa attgctaaat ggcagcagaa gatgaaaaca
    18541 tctgaaaagc aagctaaaaa agcgcaaaat atgattaatg atgcgcaaaa taaaattgct
    18601 aaaaatgaag atttaaaacg agacctagaa gctttagaaa aggaacatcc tgaaggtatt
    18661 tatctttctg gtgcactatt aatgtttgct ggctcaaaat catattactt atatggtgcg
    18721 tcttctaatg aatttagaga ttttttacca aatcatcata tgcagtatac gatgatgaag
    18781 tatgcacgtg aacatggtgc aacaacttac gatttcggtg gtacagataa tgatccagat
    18841 aaagactcag aacattatgg attatgggca tttaaaaaag tgtggggaac atacttaagt
    18901 gaaaagattg gtgaatttga ttatgtattg aatcagccat tgtaccaatt aattgagcaa
    18961 gttaaaccgc gtttaacaaa agctaaaatt aaaatatctc gtaaattaaa acgaaaatag
    19021 attaacgact gaaatctgaa cgctcataag actgtcattt gcgttcagat ttttttacac
    19081 aatatagaat ggttgagtaa aatatttttg aatatagtga aagaggggga agtactgtga
    19141 taaaaaagct attacaattt tctttaggga ataagtttgc tatcttttta atggttgttt
    19201 tagttgtctt gggcggtgta tatgcgagtg ctaaattgaa attagaatta ctaccaaatg
    19261 tacaaaatcc agttatttca gttacaacaa caatgccggg tgcaacgcca caaagtaccc
    19321 aagatgaaat aagtagtaaa attgacaatc aagtaagatc attggcatat gtgaaaaatg
    19381 ttaaaacgca atccatacaa aatgcttcaa ttgtaacagt tgaatatgaa aataatacag
    19441 atatggataa agcagaagaa cagcttaaaa aagaaatcga taaaattaaa tttaaagatg
    19501 aagttggtca accagaatta agacgtaatt cgatggatgc ttttccggtt ttagcatatt
    19561 cattttcaaa taaagagaat gacttgaaaa aagtaacgaa agtactgaat gaacaattaa
    19621 taccaaaatt gcaaacggta gatggtgtgc aaaatgcgca attaaatggg cagacgaacc
    19681 gtgaaatcac ccttaaattt aagcaaaatg aacttgaaaa atatgggttg actgctgatg
    19741 atgtagaaaa ctatctaaaa acggcaacaa gaacaacgcc acttggattg ttccaatttg
    19801 gtgataaaga taaatcaatt gttgttgatg gtcaatatca atctgttgat gcttttaaaa
    19861 acataaatat tccattaacg ttggcaggag gacaagggca atctcaatcc caaagtgaca
    19921 ataaacaaaa ttcagccatg tcagacgtta atcaggcatc accacagcaa aattcaaaag
    19981 cgtcagcacc aaataatata agtggtatgc cgacagcgaa actaggagat ttagctgata
    20041 ttacagttgg tgatgtgcgt acttctattt ctaaaacgaa tggaaaggat gcggttaatc
    20101 tacaaataac taaagctcaa gatgccaata cagttcaagt agccaaagat gtacaacgta
    20161 aaattgatac attcgttgat gaaaataaag attttaatgt cacaaaaaca atggatactg
    20221 caaagcctgt tgagaaatca ctttatacga tggttgaaaa agcatcatta ggtacaatcg
    20281 tggcaattat agttattttg ctgtttttaa gaaacattcg tacgacggca atttctatta
    20341 tatcgattcc gttatcactt cttatggcgc ttattgctct gaaattgagt gatgtttcat
    20401 tgaatatact aacgttaggt gcattaacag tagcgattgg acgtgtgata gacgattcga
    20461 ttgtagttgt tgaaaatatt tatcgacgct taacagattc agaagaacaa ctaaaaggtg
    20521 aaaatttaat tatcagtgcg acaactgaag tatttaaacc aataatgtca tcgacactag
    20581 ttactattat cgtcttctta ccacttgtgt ttgtatcagg ttcagtaggc gaaatgttta
    20641 gaccttttgc attggctatt gcatttagtt tattagcatc gttattagtg tcaattacac
    20701 tcgttccagc gttggcagct acactattta aaaaaggcgt taaacgtcgt aataaacaac
    20761 atcaagaagg attaggtgtt gttagtacaa cttataaaaa agtattacat tggtcattaa
    20821 atcataagtg gattgtaatt atattaagta cattaatttt ggttgcaact attgtatttg
    20881 gaggaccgag actaggcact agctttattt cagcaggtga cgataaattt ttagctatta
    20941 cttatacacc gaagcctggt gaaacggagc aagcagtgtt gaatcatgcg aaagatgttg
    21001 aaaaatattt aaaacagaaa aagcatgtaa aaacaattca atactcagtt ggcggtagta
    21061 gtccagtaga tccaacgggt agtacaaata gtatggcaat catggttgaa tatgataatg
    21121 acacgcctaa ttttgatgta gaagcggata aggttattaa acatgcagat ggctttaaac
    21181 atcctggaga gtggaaaaat caagatttag gaacaggtgc aggtaataaa tctgtagagg
    21241 ttactgtaaa aggtccatca atggatgcca taaaatcaac tgtaaaagat attgaacaga
    21301 aaatgaaaca ggttaaagga ctagccaatg tcaaatctga tttatcgcaa acatatgatc
    21361 agtatgaaat taaagtcgat caaaataaag cggcagaaaa tggtatttct gcaagtcaac
    21421 ttgcaatgca cttgaatgaa aacttaccag aaaaaacagt tacgactgtt aaagaaaatg
    21481 gtaaaactgt tgatgttaaa gtcaaacaaa ataagcaaac agactggtca gaagataagt
    21541 tgaataatat aactttgaaa aagccgactg gtggtacgat taaattggga gatatcgcta
    21601 cgttagttaa aacaacgaca ccaagtaaat tgacgcaaga acaaggagat tatgcaacga
    21661 cggtatctgc taaagtaaca aataaagatg tgggtggcac aacacgacaa gtgatgtcta
    21721 aaataaataa tttggacaaa ccgaataatg taaaggttaa tatcggtggt gcatcagatg
    21781 atattaacaa tgcaatgaca caattagcct ttgcaatgtt agctgcaatc attatcgtat
    21841 atttaatcct agttattaca tttaaaggtg gcctagcacc atttacaatt ttattctctt
    21901 taccatttac agttatcggt gtaattattg cactattaat cacaggagaa acaatatcag
    21961 taccaagttt aattggtatg ctaatgttaa ttggaatcgt agtaacaaat gccattgtgt
    22021 taatagaccg tgttattaat aatgagcaac agggcatgga gatgaaagaa gcattaatcg
    22081 aagcaggcgg tactagaatt agaccgatat taatgacggc gattgcaaca attggtgcat
    22141 tagttccttt gttatttggt caagatagct cgattcttat ttcgaaagga ttagctgcta
    22201 cagttattgg tggtttaatt tcatcaactt tattaacact tgtagttgta ccagtaatat
    22261 atgaaattct ctttacttta aaaaaacgat tcactaaacg atagataata taaaagccat
    22321 aaaagcggtc atgatattga ctacttttat ggctttttaa aatttgaaat tttagacctt
    22381 atttcacgtg cgatttaatc gatgtgttaa taaaacaata ctggcattca acatgtaatc
    22441 cgataataaa ataaggttgc aattatgtta ttgcatattt tgtttattac ttagaataat
    22501 acgatggtta agatgaaata tatgacaaag ataaataatg acattaaatt caagatgttt
    22561 acgcctttta gatctttttt aagcatttta aatcctgtca cttcgacatt accgactaag
    22621 agtaataaga cgataaatac ccaccaattc tcttttggaa aactcatcgt cgttaatgct
    22681 aagacgaata ataacaacac acccataata attctcaaaa tgcgagtaaa tacaacttcc
    22741 attttaaaaa tactaataaa actaacaatt agataaagta ttgctagtaa aatcagataa
    22801 aattgcatat gataacttcc tctccgtttc aaagggttca tgtagtacag cagcgtagaa
    22861 aacataaata gcagtgttgg gatagatatg cgtgtatgtt gttaatgaaa cactaaatca
    22921 taacacgaca acatcttgtg attctagatg taattgagcg aaaaatgaag caattcatca
    22981 atataacgta ttcagagcaa tgctagtcaa atttatgtaa caaaaagcat ttattgcttg
    23041 aatgatagat tattaatctt gcatttgagc acgaacgttt ctaatttcat tttcaatatc
    23101 tttaagttga cgaacaagtg cggaagtatc taaattcaaa gattcccgtt tttctatgtc
    23161 accttgaaga cgaacataat catatttaag ttcggctagt tggttttcta aagacatact
    23221 ataacactcc tttgaaatat aattaagtgc atttaatgta gattatagca tgaaacagta
    23281 gacttc
//
LOCUS       DAKTGS010000029        22944 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.029, whole genome shotgun sequence.
ACCESSION   DAKTGS010000029 DAKTGS010000000
VERSION     DAKTGS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.029"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            140..274
                     /locus_tag="O0286_000967"
     CDS             140..274
                     /locus_tag="O0286_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0169353.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            331..465
                     /locus_tag="O0286_000968"
     CDS             331..465
                     /locus_tag="O0286_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0169354.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            589..1284
                     /locus_tag="O0286_000969"
     CDS             589..1284
                     /locus_tag="O0286_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCY0169355.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(1393..1833)
                     /locus_tag="O0286_000970"
     CDS             complement(1393..1833)
                     /locus_tag="O0286_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0169356.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            2028..3641
                     /gene="bshC"
                     /locus_tag="O0286_000971"
     CDS             2028..3641
                     /gene="bshC"
                     /locus_tag="O0286_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCY0169357.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            3785..4216
                     /gene="mraZ"
                     /locus_tag="O0286_000972"
     CDS             3785..4216
                     /gene="mraZ"
                     /locus_tag="O0286_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCY0169358.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            4232..5167
                     /gene="rsmH"
                     /locus_tag="O0286_000973"
     CDS             4232..5167
                     /gene="rsmH"
                     /locus_tag="O0286_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCY0169359.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            5181..5582
                     /gene="ftsL"
                     /locus_tag="O0286_000974"
     CDS             5181..5582
                     /gene="ftsL"
                     /locus_tag="O0286_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCY0169360.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            5563..7797
                     /locus_tag="O0286_000975"
     CDS             5563..7797
                     /locus_tag="O0286_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0169361.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            8089..9054
                     /locus_tag="O0286_000976"
     CDS             8089..9054
                     /locus_tag="O0286_000976"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCY0169362.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            9056..10405
                     /locus_tag="O0286_000977"
     CDS             9056..10405
                     /locus_tag="O0286_000977"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCY0169363.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            10421..11740
                     /locus_tag="O0286_000978"
     CDS             10421..11740
                     /locus_tag="O0286_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCY0169364.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            11846..13258
                     /gene="ftsA"
                     /locus_tag="O0286_000979"
     CDS             11846..13258
                     /gene="ftsA"
                     /locus_tag="O0286_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCY0169365.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            13291..14463
                     /gene="ftsZ"
                     /locus_tag="O0286_000980"
     CDS             13291..14463
                     /gene="ftsZ"
                     /locus_tag="O0286_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCY0169366.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            14723..15514
                     /gene="pgeF"
                     /locus_tag="O0286_000981"
     CDS             14723..15514
                     /gene="pgeF"
                     /locus_tag="O0286_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCY0169367.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            15538..16206
                     /locus_tag="O0286_000982"
     CDS             15538..16206
                     /locus_tag="O0286_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCY0169368.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            16203..16766
                     /locus_tag="O0286_000983"
     CDS             16203..16766
                     /locus_tag="O0286_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCY0169369.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            16778..17068
                     /locus_tag="O0286_000984"
     CDS             16778..17068
                     /locus_tag="O0286_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCY0169370.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            17178..17957
                     /locus_tag="O0286_000985"
     CDS             17178..17957
                     /locus_tag="O0286_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCY0169371.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIIGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            17981..18598
                     /locus_tag="O0286_000986"
     CDS             17981..18598
                     /locus_tag="O0286_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCY0169372.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            18819..21572
                     /gene="ileS"
                     /locus_tag="O0286_000987"
     CDS             18819..21572
                     /gene="ileS"
                     /locus_tag="O0286_000987"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCY0169373.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            21862..22659
                     /locus_tag="O0286_000988"
     CDS             21862..22659
                     /locus_tag="O0286_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0169374.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
     gene            complement(22720..22935)
                     /locus_tag="O0286_000989"
                     /pseudo
     CDS             complement(22720..22935)
                     /locus_tag="O0286_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8443 a         3159 c         4362 g         6980 t
ORIGIN      
        1 tgtgcggata aacaaactaa ttgtatcaaa tttattttaa aataacaaca acaaaacgat
       61 aagcgaataa catttcggtg atttaaaagc tacgcacgtt tttgttatct tcaaatttaa
      121 attttaagga gtgttttcaa tggaaggttt atttaacgca attaaagata ccgtaactgc
      181 agcaattaat aatgatggcg caaaattagg cacaagcatt gtgagcatcg ttgaaaatgg
      241 cgtaggttta ttaggtaaat tattcggatt ctaatttcaa tatgttatgt aagtaatcag
      301 tattatttca aaggtgaggg agagatttaa atgactggac tagcagaagc aatcgcaaat
      361 actgtgcaag ctgcacaaca acatgatagt gtgaaattag gcacaagtat cgtagacatc
      421 gttgctaacg gtgtgggttt actaggtaaa ttatttggat tctaatataa taactaatat
      481 tctttaaaat aaactgggtg agcatacttt aatgttatgc actcagttta ttttatttgc
      541 agaaatttga gcctctgtta agatttagat acatagacaa tataggagat ggggaaattg
      601 ggatataaaa atattttgat agactttgat gatacaattg ttgattttta tgatgcagaa
      661 gaatgggcgt ttcactatat ggcgaatgtt tttaatcata aagcaacaaa ggatgatttt
      721 ttaacattta aaaaaatcaa tcaccaacat tgggaagctt ttcaacaaaa taaattaacg
      781 aagtctgaag tattatcaga acgatttgtg aattacttca aacatcatca aatggaagtt
      841 gatgggcatc gtgcagatgt gttatttaga aatggattag cagaagctaa agttaaatac
      901 tttgatcaaa cattagaaac aattgtcgaa ttatcgaaaa gacatgattt atatattgtt
      961 actaatggtg taaccgaaac gcaaaagaga aggttaaatc agacgccgtt gcataaatat
     1021 attaaaaaga tatttatatc tgaagaaaca ggatatcaaa aacctaatcc ggaatttttt
     1081 aattatgttt ttaatgatat tggtgaggat gaaagacagc actcgattat agttggagat
     1141 tctttaacat ctgacattct aggtggaatc aatgcgggta tagctacttg ctggtttaat
     1201 tttagaggat ttgatcataa tccaggaatt atacctgatt atgaaattaa ttcatggaaa
     1261 caactaaatg atattgtacg ttaagacaaa taatttgaaa atgatttact tatattagct
     1321 taagaaagta attgtaaaaa agacactacg aaaagatgca ttttcatgct cgtagtgtcg
     1381 aatattatta tcttatggtt tccacaataa gacatcatgt ccctctatat ttttagcttc
     1441 cacatctgta aatccatgat gtaaaaagaa atccttagaa tcatttctgc caatggcttt
     1501 aattggcata ttgaaactct ttgcaaaatc aatcaattct gaagcgtacc ctctgttttg
     1561 atattttggt aatacttcta acttccataa taatatataa tcttcaaaat ctgggaagta
     1621 gatttcttcg acatcacctt tttttagtaa tgccattcta gctcctaatt gatctccgac
     1681 aaatatgcca taaaatggtg aatctgaact tgcatcaatc atttgaccgt ttaactcatt
     1741 gaccatgtat aagtctttgt tgccaaacgc tctaaagttt tcgaataatt cgtcagtttt
     1801 gtaattaatt tcaagacgtt tgatttcact catatttatc taccccttat ttgtttacta
     1861 ctattattat acattaaacc acttgtgttt ttcattgatt tgaaaatgta aaacaatgaa
     1921 tattataaat tttccatgat taaattgatt ttcaagccgt aaaattgtca atcaccggta
     1981 tagactttat actatagttt gtaatcgcgt acttaaggag aaaaaacatg gactgtaaag
     2041 tagttagttt aaatgaaaaa gatcagttta taccaaaaat aaagagcagt gaccctgtaa
     2101 taacaggatt atttcaatat gatgcagctc aacaaactag ttttgaaaaa aggatgtcta
     2161 aagaaaataa tggaagagaa gcggcattag cgaatgttat tcgtgaatat atgagtgatt
     2221 taaagctttc aagtgaacaa gaattaaaca tacaacattt agctaatggt tcaaaagttg
     2281 tgattggtgg acaacaagca gggcttttcg ggggaccatt gtatacattc cataaaatat
     2341 tttcaatcat tactttatct aaggaattaa cggatacaca taagcaacaa gtagtaccag
     2401 ttttttggat tgcaggagaa gatcatgatt tcgatgaagt gaatcataca tttgtttata
     2461 acgaaaatca tgggtcgctg cataaggtta aatatcatac aatggagatg ccagagacga
     2521 ctgtctctag atattatcct gataaggctg agttgaaaca aactttaaaa acgatgttca
     2581 ttcatatgaa agaaactgtt catacacaag gtctactgga gatttgtgac agaattattg
     2641 accaatatga ctcgtggact gatatgttta aagcactact gcatgaaaca tttaaagcat
     2701 atggcgttct atttatagat gcgcagtttg agccgttaag aaaaatggaa gcgcctatgt
     2761 ttaaaaagat tttgaaaaaa catcagttgc ttgatgatgc ttttagagca acacaacaac
     2821 gtactcaaaa tcaaggcttg aatgcgatga tacaaacaga tacaaatgtt catttattct
     2881 tacatgatga aaatatgcgt caattagttt cgtatgatgg taagcatttt aaattaaata
     2941 aaacagataa gacatatata aaggaagaaa ttataaatat tgcggaaaat caacctgaat
     3001 tattttctaa taatgtagtg acaagaccat taatggaaga atggttattt aacacggtgg
     3061 catttgttgg aggaccgagt gaaattaagt actgggctga actaaaagat gtatttgaac
     3121 tatttgatgt tgaaatgcct atcgtgatgc caaggcttag aattacttat ttaaatgacc
     3181 gtatagaaaa attactttcg aaatacaata ttccattaga aaaagtgtta gtcgatggtg
     3241 ttgaaggaga aagaagtaag tttattagag aacaagcatc acatcaattt attgaaaagg
     3301 tagaaggtat gattgaacaa cagcgtcgtc taaacaaaga cttattagat gaagtggcgg
     3361 ggaatcaaaa taatattaac cttgtgaata aaaataatga aattcatata caacagtatg
     3421 attatttgtt aaaacgttat cttttaaaca ttgaaagaga aaacgacatc agtatgaagc
     3481 aatttagaga aattcaagaa acactccatc caatgggagg attacaagaa agaatatgga
     3541 atccacttca aattttgaat gattttggga cagatgtgtt caagccctcc acctatccac
     3601 cactttctta cacttttgat catattatta taaaacctta atataccaag ggtttagccc
     3661 gatttatctt aatgataaat cgggcatttt tttgtttttt aaaataaatt tcacaaattt
     3721 tgtataaata gtggtggata gtggggagat gtggtaaatt atatataagg tgaggtgata
     3781 aaaaatgttc atgggagaat acgatcatca attagataca aaaggacgta tgattatacc
     3841 gtccaagttt cgttatgact taaatgagcg ttttattatc acaagaggcc ttgataaatg
     3901 tttattcggt tacactctag acgaatggca acagattgaa gagaaaatga aaaccttacc
     3961 tatgacaaaa aaagacgcac gtaagtttat gcgtatgttc ttctctggtg ctgttgaagt
     4021 agaacttgat aagcaagggc gtattaacat ccctcaaaac ttgaggaaat acgctaattt
     4081 aactaaagaa tgtacagtaa tcggtgtttc aaatcgtatt gagatttggg atagagaaac
     4141 ttggaatgat ttctatgaag aatctgaaga aagtttcgaa gatattgctg aagatttaat
     4201 agattttgat ttttaaaatg gaggaattga agtgtttcat catatcagcg ttatgttaaa
     4261 cgaaaccatt gattatttaa atgtaaaaga aaatggtgtg tacattgact gtacgctagg
     4321 tggagcggga catgcccttt atttactaaa tcaattaaat gacgacggaa gattaatagc
     4381 aatcgatcaa gaccaaactg caattgataa tgctaaagag gtattaaagg atcatttgca
     4441 taaggtgact tttgttcata gcaacttccg tgaattaact caaatattaa aagacttaaa
     4501 cattgaaaaa gtagatggaa tttattacga cttgggtgtt tcaagcccac aactcgacat
     4561 tccagaacga ggattcagtt atcaccatga cgcaacatta gacatgcgta tggaccaaac
     4621 acaagaacta acagcatatg aaattgttaa caattggtca tatgaagcgt tagtgaagat
     4681 tttttatcgc tatggcgagg agaaattttc aaaacagata gctcgaagaa tcgaagcaca
     4741 tcgcgaacaa caaccaataa caacaacatt agaattagtt gacattataa aagaaggtat
     4801 tcctgcaaaa gcaagaagaa aaggcggaca tcctgcaaaa cgagtatttc aagcactacg
     4861 aattgcagta aacgatgaat tgtcagcttt tgaagattca atagaacaag cgattgaatt
     4921 agtgaaagta gatggcagga tttcggtaat cactttccat tctttagaag atcgtttatg
     4981 taaacaggtg ttccaagaat atgaaaaagg tccagaggta ccaagaggat taccagttat
     5041 accagaagca tatacaccta agttaaagcg tgttaatcgt aaaccgatta ccgctacaga
     5101 agaagattta gatgacaata acagagcacg aagcgcgaaa ttacgtgtag ctgaaatact
     5161 taaataagga gcaatttata atggctgtag aaaaagtgta ccaaccatat gacgaacaag
     5221 tttataatag tataccgaag caacaaccac aaactaagcc cgaaaagaag actgtttcga
     5281 gaaaagtggt tgtacaatta actaaatttg aaaaagtttt atacataact ttgattactg
     5341 taattgctat gttaagtatt tatatgctat ctttaaaaat ggatgcgtat gatacgcgag
     5401 gaaagattgc agatttagat tataaaatag ataaacaatc aagtgaaaac agtgctttac
     5461 aatctgaaat caaaaagaat tcttcttatg aacgcatata cgaaaaggct aagaaacagg
     5521 ggatgagcct tgagaacgat aatgtaaagg tagtgcgtag taatggcgaa gcaaaaaatt
     5581 aaaattaaaa aaaataaaat aggggcagtc ctacttgttg gtttattcgg actgctcttt
     5641 tttatattgg ttttaagaat ttcatatatc atgattactg gacattctaa tggtcaagat
     5701 ttagtcatga aggcaaatga aaagtattta gttaagaatg cacaacaacc agaacgagga
     5761 aagatatatg atcgtaatgg taaagtgcta gcagaagatg tagaaagata taaacttgtt
     5821 gcagtaatag ataaaaaggc gagtgccaat tctaaaaaac ctaggcatgt agttgataaa
     5881 aaagagactg caaagaaatt atctacagtc attaatatga agccagagga aattgaaaag
     5941 agacttagtc aaaagaaagc tttccaaatt gaatttggac gcaaaggaac aaatttaacg
     6001 tatcaggaca aattgaaaat agagaaaatg aatttgcctg gtatttcttt attgcctgaa
     6061 acagaacgct tttatccaaa tggcaatttt gcatcacact taattggtag agctcagaaa
     6121 aatccggata ctggtgaact taaaggtgca cttggagttg aaaagatttt tgatagttat
     6181 ttaagtggat ctaaaggatc attgagatat attcatgata tttggggata tatcgcacca
     6241 aatactaaaa aagagaagca gcctaaacgt ggtgatgatg tccatttaac aatcgattca
     6301 aatattcaag tatttgttga agaagcttta gatggcatgg ttgaaagata ccagccgaaa
     6361 gatttatttg cggttgtcat ggatgccaaa actggagaaa ttttagcata cagtcagcga
     6421 ccaacattta atcctgaaac tggtaaagac tttggtaaaa agtgggcaaa tgacctttat
     6481 caaaacacat acgagcctgg atcaacattt aaatcatatg ggttagcagc tgctattcaa
     6541 gaaggtgctt ttgatcctga taagaaatat aaatctggac atagagatat tatgggttca
     6601 cgtatttcag actggaatag agtcggttgg ggtgaaatcc caatgtcact cggatttact
     6661 tattcatcta atacattgat gatgcattta caagatttag ttggtgcaga caaaatgaaa
     6721 tcttggtatg aacgatttgg atttggaaaa tcaactaaag gtatgtttga tggagaagca
     6781 cctggtcaaa ttggatggag taatgagttg caacaaaaaa cgtcatcatt tggtcaatcg
     6841 acaacagtaa cacctgttca aatgttacaa gcgcaatcag cgttctttaa tgatggtaat
     6901 atgttaaaac catggtttgt gaatagcgtt gaaaatcctg ttagtaaaag acaattttat
     6961 aaagggcaaa aacaaatcgc aggcaaacca ataacaaaag atactgctga aaaagttgaa
     7021 aagcaattgg atttagttgt gaatagtaag aagagtcacg ctgcaaacta tcgtattgat
     7081 ggttatgagg tcgaaggtaa gactggtaca gcacaagtcg ctgcacctaa tggtggtgga
     7141 tacgttaaag gtccaaaccc atattttgta agttttatgg gtgacgcgcc gaagaaaaat
     7201 cctaaagtta ttgtatacgc tggtatgagc ttggcacaaa aaaatgacca agaagcttat
     7261 gaattaggtg ttagtaaagc gtttaaacca ataatggaaa atactttgaa atatttaaat
     7321 gtaggtaaat caaaagatga cacatctaat gcagagtata gtaaagtgcc agatgttgaa
     7381 ggtcaagaca aacaaaaagc tattgataat gtgagtgcaa aatcattaga accagttact
     7441 attggttctg gcacacaaat aaaagcacaa tctataaaag cagggaataa agtcttacct
     7501 catagtaaag tactgttatt aacagatgga gacttaacta tgcctgacat gtcaggatgg
     7561 acgaaagaag atgtcattgc ttttgaaaac ctaacaaata ttaaagtaaa tttaaaaggt
     7621 agcggttttg tgtcccacca atcaattagt aagggacaaa aacttactga aaaagataaa
     7681 atagacgtag aattttcatc agagaatgta gacagcaatt cgacgaataa ttctgattca
     7741 aattcagatg ataagaagaa atctgacagt aaaactgaca aggataagtc ggactaacaa
     7801 gaatggctac attaaattag gatgtattgt cactatttgt ttttactaaa gtaatgctgt
     7861 gatagaaaac aacatcttca tttaatcttt ataaataagt gttgatagag aattttaagg
     7921 tagacgaggt cagattaact tgtttgttat aggcaaactt tttatactgg cctcttatgc
     7981 cgtaaaaaag aaaaattata aattttgatt acaactttgt ttatttaatt gaaaaattga
     8041 taatatatta gtggttaaaa aagtctggat taagaagagg agatagttat gatttttgta
     8101 tatgcgttat tagcgctagt gattacattt gttttggtac ctgttttaat acctacatta
     8161 aaaaggatga aatttggtca aagtattcga gaagaaggtc cacaaagcca tatgaagaag
     8221 actggtacac caacgatggg tggactaaca tttctattaa gtattgtgat aacgtctttg
     8281 gtggctatta tatttgtaga tcaagctaat ccaatcatac tgttattatt tgtgacgatt
     8341 ggttttgggt taattggttt tatagatgat tatattattg ttgttaaaaa gaataaccaa
     8401 ggtttaacaa gtaaacagaa gtttttggcg caaattggta ttgcgattat attctttgtt
     8461 ttaagtaatg tgtttcattt ggtgaatttt tctacgagca tacatattcc atttacgaat
     8521 gtagcaatcc cactatcatt tgcatatgtt attttcattg ttttttggca agtaggtttt
     8581 tctaatgcag taaatttaac agatggttta gatggattag caactggact gtcaattatc
     8641 ggatttacaa tgtatgccat catgagcttt gtgttaggag aaacggcaat tggtattttc
     8701 tgtatcatta tgttgtttgc acttttagga tttttaccat ataacattaa ccctgctaaa
     8761 gtgtttatgg gagatacagg tagcttagct ttaggtggta tatttgctac catttcaatc
     8821 atgcttaatc aggaattatc attaattttt ataggtttag tattcgtaat tgaaacatta
     8881 tctgttatgt tacaagtcgc tagctttaaa ttgactggaa agcgtatatt taaaatgagt
     8941 ccgattcatc atcattttga attgatagga tggagcgaat ggaaagtagt tacagtattt
     9001 tgggctgttg gtctgatttc aggtttaatc ggtttatgga ttggagtgca ttaagatgct
     9061 taattataca gggttagaaa ataaaaatgt attagttgtc ggtttggcaa aaagtggtta
     9121 tgaagcagct aaattattaa gtaaattagg tgcgaatgta actgtcaatg atggaaaaga
     9181 cttatcacaa gatgctcatg caaaagattt agaatctatg ggcatttctg ttgtaagtgg
     9241 aagtcatcca ttaacgttgc ttgataataa tccaataatt gttaaaaatc ctggaatacc
     9301 ttatacagta tctattattg atgaagcagt gaaacgaggt ttgaaaattt taacagaagt
     9361 tgagttaagt tatctaatct ctgaagcacc aatcatagct gtaacgggta caaatggtaa
     9421 aacgacagtt acttctctaa ttggagatat gtttaaaaaa agtcgcttaa ctggaagatt
     9481 atccggcaat attggttatg ttgcatctaa agtagcacaa gaagtaaagc ctacagatta
     9541 tttagttaca gagttgtcgt cattccagtt acttggaatc gaaaagtata aaccacacat
     9601 tgctataatt actaacattt attcggcgca tctagattac catgaaaatt tagaaaacta
     9661 tcaaaatgct aaaaagcaaa tatataaaaa tcaaacggaa gaggattatt tgatttgtaa
     9721 ttatcatcaa agacaagtga tagagtcgga agaattaaaa gctaagacat tgtatttctc
     9781 aactcaacaa gaagttgatg gtatttatat taaagatggt tttatcgttt ataaaggtgt
     9841 tcgtattatt aacactgaag atctagtatt gcctggtgaa cataatttag aaaatatatt
     9901 agcagctgtg cttgcttgta ttttagctgg tgtacctatt aaagcaatta ttgatagttt
     9961 aactacattt tcaggaatag agcatagatt gcaatatgtt ggtactaata gaactaataa
    10021 atattataat gattccaaag caacaaacac gctagcaaca cagtttgcct taaattcatt
    10081 taatcaacca atcatttggt tatgtggtgg tttggatcga gggaatgaat ttgacgaact
    10141 cattccttat atggaaaatg ttcgcgcgat ggttgtattc ggacaaacga aagctaagtt
    10201 tgctaaacta ggtaatagtc aagggaaatc ggtcattgaa gcgaacaatg tcgaagacgc
    10261 tgttgataaa gtacaagata ttatagaacc aaatgatgtt gtattattgt cacctgcttg
    10321 tgcgagttgg gatcaatata gtacttttga agagcgtgga gagaaattta ttgaaagatt
    10381 ccgtgcccat ttaccatctt attaaagggt gtgagtattg atggatgata aaacgaagaa
    10441 cgatcaacaa gaatcaaatg aagataaaga tgaattagaa ttatttacga ggaatacatc
    10501 taagaaaaga cggcaaagaa aaagatcaaa ggctacacat ttttctaatc aaaataaaga
    10561 tgatacatct caacaagctg attttgatga agaaatttac ttgataaata aagacttcaa
    10621 aaaagaagaa agcaatgata aaaataatga ttctgcttct agtcatgcga atgataataa
    10681 tatcgatgat tctacagact ctaatattga aaatgaggat tatagatata atcaagaaat
    10741 tgacgaccaa aatgaatcga atgtaatttc agtcgacaac gaacaacctc aatcagctcc
    10801 taaagaacaa aatagcgact cgattgatga ggaaacagta acgaaaaaag aacgaaaaag
    10861 taaagtaaca caattaaagc cattaacact tgaagaaaag cggaagttaa gacgtaagcg
    10921 acaaaagcga atccaataca gtgttattac aatattggta ttgttgattg ctgttatatt
    10981 aatttacatg ttttcaccac ttagtaaaat tgcgcatgta aatataaatg gaaataatca
    11041 cgttagtact tcaaagataa acaaagtttt aggtgttaaa aatgattcaa ggatgtatac
    11101 gtttagtaaa aaaaatgcta ttaatgatct cgaagagaat ccattaatca aaagtgttga
    11161 gatacacaag caattaccaa acacattaaa cgtagatatc acagaaaatg aaattattgc
    11221 tttagtgaaa tataaaggta aatatttacc tttattagaa aatggtaaat tgcttaaagg
    11281 ttcaaatgat gtcaaaatta atgatgcacc tgtcatggat ggtttcaaag gtacaaaaga
    11341 agatgatatg attaaggcgt tatctgaaat gacacctgaa gttagacgat atattgccga
    11401 agtgacatac gccccaagta aaaacaaaca aagcagaatt gaattgttta cgacagatgg
    11461 acttcaagta atcggtgata tttcgacgat atctaagaaa atgaaatatt atccgcagat
    11521 gtcacaatca ttatcaaggg atagttcggg taaactaaaa acacgaggct atattgattt
    11581 atcagtcggt gcttcattta tcccataccg tggaaacacg tctagtcaat cagaaagcga
    11641 taaaaatgtg actaaatcat ctcaagagga aaatcaagca aaagaagaat tacaaagcgt
    11701 tttaaacaaa attaacaaac aatcaagtaa gaataattaa aaaattttga tattgtctat
    11761 gtttatagtt cacaagccat tcaacgtatt gtaaactaag gatagtgtat ttttttaata
    11821 gtaatttgtc aggaggtgcc tatctatgga agaacattac tacgtaagta ttgatattgg
    11881 atcatcaagc gtaaaaacaa tagtaggcga gaaatttcac aatggtataa atgtgatagg
    11941 tacaggacaa acctacacga gcggtataaa aaatggttta attgatgatt ttgatattgc
    12001 gcgacaagca atcaaagaca caattaaaaa ggcatcaatc gcttcgggtg ttgatattaa
    12061 agaagttttc ctgaaattac ctatcattgg aacggaagtt tatgatgaat caaatgaaat
    12121 cgacttttat gaggatacag aaatcaacgg ttcacatatc gaaaaagtat tagaaggtat
    12181 tagagaaaaa aatgatgtgc aagaaacaga agtaattaat gtgttcccga ttcgttttat
    12241 agtcgataaa gaaaatgagg tttcagaccc taaagaatta attgccagac attcattaaa
    12301 ggttgaagca ggcgtaattg ctattcaaaa atcgatttta attaatatga ttaaatgcgt
    12361 agaagcatgt ggtgttgatg tattagatgt ttactctgat gcatataact atggttcaat
    12421 cctaacagct actgaaaaag agttaggtgc atgtgtcatt gatattggtg aagacgttac
    12481 gcaagttgct ttttatgaac gcggtgaatt agtagatgct gattctatcg aaatggcagg
    12541 gcgtgatatt acagacgata ttgcacaagg attaaacact tcttatgaaa ctgctgaaaa
    12601 agttaaacac caatatggtc atgcattcta tgattctgct tcagatcaag atatcttcac
    12661 tgttgaacag gttgatagtg atgaaacagt acagtatact caaaaagatt tgagtgactt
    12721 tattgaagcg cgtgtagaag aaatattctt cgaagtattt gatgttttac aagatttagg
    12781 attaacaaaa gtaaatggtg ggtttattgt aactggtgga tctgcaaact tacttggcgt
    12841 aaaagaatta ttatcagata tggtaagtga aaaagttaga attcacacgc catcacaaat
    12901 gggaattaga aaacctgaat tttcttcagc aatttctaca atttctagta gtatcgcttt
    12961 tgatgagtta ttagattatg ttacaattaa ttatcatgat aatgaagaaa ctgaagaaga
    13021 tgttattgat gtgaaagaca aagataacga atctaaatta ggcggatttg attggtttaa
    13081 acgtaaaaca aacaaaaaag atactcatga aaatgaagta gagtcaacag atgaagaaat
    13141 ttatcaatca gaagataatc atcaggaaca taaacagaat catgaacatg ttcaagacaa
    13201 agataaagat aaagaagaaa gtaaattcaa aaaactaatg aaatctctat ttgaatgatt
    13261 attggccaat aaaactagga ggaaatttaa atgttagaat ttgaacaagg atttaatcat
    13321 ttagcgactt taaaggtcat tggtgtaggt ggtggcggta acaacgccgt aaaccgaatg
    13381 attgaccacg gaatgaataa tgttgaattt atcgctatca acacagacgg tcaagcttta
    13441 aacttatcta aagctgaatc taaaatccaa atcggtgaaa aattaacacg tggtttagga
    13501 gcaggagcta atcctgaaat cggtaaaaaa gctgcagagg aatctcgtga acaaattgaa
    13561 gatgcaatcc aaggtgcaga catggtattt gttacttctg gtatgggtgg cggaactggt
    13621 actggtgcag caccagtcgt tgctaaaatt gcaaaagaaa tgggcgcatt aactgttggt
    13681 gttgtaactc gtccatttag ttttgaagga cgtaaacgtc aaactcaagc tgctgctgga
    13741 gtagaagcta tgaaagctgc agtagataca ttaatcgtta taccaaatga ccgtttatta
    13801 gatatcgttg acaaatctac gccaatgatg gaagcattta aagaagctga caacgtgtta
    13861 cgccaaggtg tacaaggtat ctcagactta atcgctgttt ctggtgaagt aaacttagac
    13921 tttgcagacg ttaagacaat tatgtctaac caaggttctg cattaatggg tattggtgtt
    13981 tcttctggtg aaaatagagc ggtagaagct gctaaaaaag caatctcttc tccattactt
    14041 gaaacatcta tcgttggtgc acaaggtgtg cttatgaata ttactggtgg cgagtcattg
    14101 tcattatttg aagcacaaga ggctgctgat attgtccaag atgctgcaga tgaagacgtt
    14161 aatatgattt tcggtacagt tattaatcct gaattacaag atgagattgt tgtaacagtt
    14221 attgcaactg gttttgatga caaaccaaca tcacatggtc gtaaatctgg tagcactgga
    14281 ttcggaacaa gcgtaaatac ttctagcaat gcaacttcta aagatgaatc attcacttca
    14341 aattcatcaa atgcacaagc aactgatagt gtaagtgaaa gaacacatac aactaaagaa
    14401 gatgatattc ctagcttcat tagaaataga gaagaaagac gttcaagaag aacaagacgt
    14461 taatcggtta atatatatac acaaataatt caacacaaat catcagataa catatctgat
    14521 gattttttta ctaattttta gaacatgtag aaggacattt aagtttttca aagttattaa
    14581 aagtgtttaa gtatcgtgtg aaaattaagt caaaaattat ttgcgcaaca ttttaacttt
    14641 aaacataaat gttatattat ataattatta actttgtaca gttagacgaa gataatttaa
    14701 atgaaatgat ggtgacgatc gagtgaatga taattttaaa aagcaaccgc atcatttaat
    14761 atatgaagag ttattacaac aaggtattac tctaggtatt acaactagag gagatggttt
    14821 aagtgactat cctaaaaatg cttttaatat ggcgagatat attgatgatc gcccatataa
    14881 tattactcaa catcaattgc aattagctga agaaattgcg tttgatagaa aaaattgggt
    14941 gtttcccatt caaacacatg aaaataaagt cgcttgtatt acaaaggatg atataggcac
    15001 aaatatagac actttaactg atgcgcttca tggtattgat gcgatgtaca catatgatag
    15061 taatgtctta ttaacgatgt gttatgcaga ctgtgtacca gtatattttt atagtacaaa
    15121 acatcatttt attgcattgg cgcatgcagg ttggcgtggt acctatactg aaattgtaaa
    15181 agaagtgcta aaacatgtga actttgattt gaaagactta catgtcgtta ttggaccatc
    15241 tacatcatca agttatgaaa ttaatgatga tattaaaaat aaatttgaaa cattgccaat
    15301 tgatagtgcc aactatattg aaactagagg acgagatcgt catggtattg atttgaaaaa
    15361 agccaatgct gcattattaa tttattatgg tgttcctaaa gaaaatattt atacgacagc
    15421 gtatgctaca tctgaacatt tagaattatt tttctcttat cgattagaaa aaggtcaaac
    15481 aggacgcatg ttagcattca ttggtcaaca gtaaacaagg aggagatatg tttgcgtgtg
    15541 aaagataatt tacaacaaat ctcaacacaa attaatgaca aaagtgaaaa aaataatttt
    15601 tcaacaaaac caaacgtgat tgcagttaca aaatatgtta caatagagcg agctaaagaa
    15661 gcgtatgagg ctggaataag acattttggt gagaatagat tggaaggctt ttttcaaaag
    15721 aaagaagcat taccatcaga tgcggtgatc cattttatag gatcattaca atctcgaaaa
    15781 gttaaggacg ttataaacga cgtagattat ttccatgctt tagatcgatt gagcttagcc
    15841 aaagaaatta acaaacgtgc agaacataaa attaaatgtt tcttgcaagt gaacgtttcg
    15901 ggagaagctt ctaaacatgg tattgcttta gaagatgttg atcagtttat agatgatctt
    15961 aaaaaatatg acaaaatcga aattgtaggt ttaatgacga tggcaccatt gacagatgat
    16021 gaagcatata ttagatcgtt atttaaacag ttacgtttga aaaaagaaga aatacaacga
    16081 ctcaatttag aatatgcgcc ttgtgatgaa ttatcaatgg gaatgagtaa tgactatctt
    16141 attgcagttg aagaaggtgc gacgtttgtt agaattggga ctaaacttgt aggagaagag
    16201 gagtgagcca cttggcttta aaagatttat ttagtggatt ttttgtaata gatgatgaag
    16261 aggaagtaga agtacctgac aaacaacaac aggtaaatga agcgccagca aaagagcagt
    16321 cacaacaaac aacaaaacaa aacgcaatca aatcagtccc tcaaaaatct gcatcaagat
    16381 atacaacaac gtcagaagaa aggaataacc gtatgtctaa ttattcaaaa aataattcac
    16441 gtaatgttgt aactatgaac aatgctacac caaacaatgc atcacaagaa agttcaaaaa
    16501 tgtgtttatt cgaaccacgt gttttttcag atacacaaga tattgctgat gagcttaaaa
    16561 accgccgtgc gacacttgtc aatttacaac gtattgataa agtatcagcg aaaagaatta
    16621 ttgatttttt aagcggtact gtttatgcaa tcggtggaga tatccaacgt gtaggtactg
    16681 atattttctt atgtacgcct gataatgtgg aagtagctgg aagcattaca gaccatattg
    16741 aaaatatgga acattcattc gactaaggag ttaacatatg gatataaatg tgctagctac
    16801 aatatttaaa ttcatccttt ttgttgttga aatttattat ttcggcatga ttatatattt
    16861 ctttacatct tgggtaccaa gtattagaga aactaaggta ggttattttt tagcgaaaat
    16921 atatgaacct ttcttacaac catttagaaa agtaattcca cctattggaa ttatcgacat
    16981 atcatcaatc gctgcaatta tcgttttagt attattccaa aaagggttac tccaaatctt
    17041 taattggatt ttaattcaat tacaataaat taagcgacat aacgaggcaa gggtacatga
    17101 tactttagcc tcgtttttga tatgcatttt tctgaatata agggcaatag atggtatttt
    17161 ataatttttt taaggtagtg attaacatag atatttatca acactttaga caggaagaat
    17221 acgaattaat tgatcagcta acggataaat gtgatcaagc ggaacagcat tatgcaccag
    17281 tattaacgca ttttttagat ccaagagggc aatatatatt ggaagtgatt tgtggcagtt
    17341 atgaagattt aaacgtatct ttttatggtg gacctaatgc tgaaagaaaa agagcaatca
    17401 tttcgccgaa ctattatgaa cctaaagaaa gcgactttga attaacttta atggaaatag
    17461 attatcctga aaaattcgtc actttaaaac atcaacatat tttagggaca ttaatgtctt
    17521 taggtatcga acgcgaacaa gttggagata taattgtgaa tgaacgaatt caatttgttt
    17581 tgacaagtag attggaatca tttattatgt tagaattaca acgtattata ggcgcatcag
    17641 ttaaacttta tactattcca gtaacagata tgatacaatc taatgagaat tggaaaaatg
    17701 aaagtgcaac agttagttct ttaaggttag atgttgttat taaagaaatg atacgtaaat
    17761 cacgtacgat tgcgaaacaa ctaatcgaaa aaaaacgtgt taaagtgaat cacactattg
    17821 ttgattcagc agattttcaa ttacaagcaa atgatttaat atccatccaa ggttttggta
    17881 gagcacacat tactgactta ggtggtaaaa ctaaaaaaga taaaacgcac attacctata
    17941 gaacattatt caaatagtaa tgatttaagg aggataacaa atgcctttta caccaaatga
    18001 aattaagaat aaagagtttt cacgtgtaaa gaatggttta gaacctactg aagttgctaa
    18061 ttttttggag caactaagca ctgaaattga acgtcttaaa gaagataaaa aacaacttga
    18121 aaaagtaatc gaagagagag atactaatat taagtcttat caagacgtgc atcaatctgt
    18181 aagtgatgct ttgatacaag ctcaaaaagc tggtgaagaa actaagcaag ctgcagagaa
    18241 acaagctgaa gcgattatag ctaaggcaga agcgcaagct aatcaaatgg ttggtgacgc
    18301 ggtagaaaaa gcacgccgtt tagcattcca gactgaagat atgaaacgtc aatcaaaagt
    18361 atttagatcg cgtttccgta tgttagttga agcgcaatta gacttattaa aaaacgaaga
    18421 ttgggattac ttgttgaatt atgatttaga cgctgaacaa gtgacgcttg aaaatattca
    18481 tcatttgcat gaaaatgatt taaagccaga tgaagttgca gcaaatgcac aaaataatgc
    18541 atcaaataca ccagacaata atcaacaatc caatgattca gaaacaacta agaagtaaga
    18601 attaaataaa gacagacgcg taatatacat ttaacttttc acagcgaatt aggtaatggt
    18661 gagagcctag taaaagcatg tatgttatat cactggcttt ttaatattta aataatgtaa
    18721 tgagagaact ctaagttgag ttaataaggg tggtaccgcg agcaatcgtc ccttttaatt
    18781 taacttagag ttttttaaat ttttaaggag tgaaaaaaat ggattacaaa gaaacgttat
    18841 taatgcctaa aacagatttc ccaatgcgag gtggtttacc aaacaaggaa ccgcaaattc
    18901 aagaaaaatg ggatgcagaa gatcaatacc ataaagcgtt agaaaaaaat aaaggtaacg
    18961 aaacattcat tttacatgat ggcccaccat acgcgaatgg taacttacat atgggacatg
    19021 ccttgaacaa aattttaaaa gactttattg tacgttataa aactatgcaa gggttctatg
    19081 caccatacgt accaggttgg gatacacatg gtttaccaat tgaacaagca ttaacgaaaa
    19141 aaggtgttga ccgaaagaaa atgtcaacag ctgaattccg tgagaaatgt aaagaatttg
    19201 ctttagaaca aattgaatta cagaaaaaag attttagacg tttaggtgtt cgtggtgact
    19261 ttaatgatcc atatattaca ttaaaacctg aatacgaagc tgcacaaatt cgtatttttg
    19321 gagaaatggc agataaaggt ttaatttata aaggtaaaaa gccagtttat tggtctcctt
    19381 caagtgagtc ttcattagca gaagcagaaa ttgaatatca cgataaacgt tcagcatcaa
    19441 tttacgttgc atttgacgtt aaagatgaca aaggtgtcgt tgatgcagat gctaaattta
    19501 ttatctggac aacaacgcca tggacaattc catcaaatgt tgcgattacc gttcatcctg
    19561 aattaaaata tggtcaatac aatgtaaatg gcgaaaaata tattattgca gaagccttgt
    19621 ctgacgctgt agcagaagca ctggattggg ataaagcatc aatcaaatta gaaaaagaat
    19681 acacaggtaa agaattagag tatgttgtag cacaacatcc attcttagac agagaatcgt
    19741 tagtgattaa tggtgatcat gttactacag atgctggtac aggttgtgta catacagcac
    19801 caggtcacgg ggaagatgac tatattgttg gtcaaaaata tgaattgcca gtaattagtc
    19861 caatcgatga taaaggtgta tttactgaag aaggcggcca atttgaaggg atgttctatg
    19921 ataaagctaa taaagccgtt actgatttat taacagaaaa aggtgcacta ttaaaattag
    19981 actttattac acatagctat ccacacgact ggagaacaaa aaaacctgta atcttccgtg
    20041 ctacaccaca atggtttgcc tcaatcagta aagtaagaca agatatttta gatgcaatcg
    20101 aaaatacaaa cttcaaagta aattggggta aaacacgtat ttacaatatg gttcgtgacc
    20161 gtggcgaatg ggttatttct cgtcaacgtg tgtggggtgt accgttacca gtattttatg
    20221 ctgaaaatgg cgaaattatc atgacgaaag aaacagtgaa tcatgttgct gatttatttg
    20281 cagaacacgg ttcaaatatt tggtttgaaa gagaagcgaa agacttacta ccagaaggat
    20341 ttacacatcc aggcagccct aacggtacat ttactaaaga aacagacatt atggacgttt
    20401 ggtttgattc tggttcatca caccgtggcg tgttggaaac aagaccggaa ttaagtttcc
    20461 cagcggatat gtatttagaa ggtagtgacc aatatcgtgg ttggttcaac tcttctatca
    20521 caacttcagt tgctacaaga ggagtatcac cttataaatt cttactttct catggttttg
    20581 ttatggacgg tgaaggtaag aaaatgagta aatctttagg taatgtgatt gtacctgacc
    20641 aagtggttaa acaaaaaggt gctgatattg cgagactttg ggtaagtagt acggactatt
    20701 tagctgatgt tagaatttct gatgaaattt taaaacaaac atctgatgtt tatcgtaaaa
    20761 tcagaaatac attaagattt atgttaggta acattaacga tttcaatcct gacacagata
    20821 gcattcctga atcagagtta ttagaagtgg atcgttactt gctaaatcgt ttacgtgaat
    20881 ttactgcaag tacgattaac aactatgaaa actttgacta cttaaatatt tatcaagaag
    20941 ttcaaaactt tatcaatgtt gagttaagta atttctattt ggattacggt aaagatattt
    21001 tatatattga acaacgtgat tctcatatcc gtcgtagtat gcaaacagtg ttatatcaaa
    21061 ttttagttga tatgacgaag ttgttagcac caatcttagt gcatacagct gaagaagttt
    21121 ggtctcatac accacatgtt aaagaagaaa gtgttcactt agcagacatg cctaaagttg
    21181 tagaagtaga tcaagcttta ttggataaat ggcgtacatt tatgaattta cgtgatgatg
    21241 tgaaccgtgc attagaaact gctcgtaatg aaaaagttat tggtaaatca ttagaagcta
    21301 aagttacgat tgctagtaac gataaattta atgcatctga attcttaact tcatttgatg
    21361 cattacatca attatttatc gtgtcacaag ttaaagttgt agataagtta gacgatcagg
    21421 caacagctta tgaacatggt gatattgtca tcgaacatgc agatggtgaa aaatgtgaaa
    21481 gatgttggaa ctattcagag gatcttggtg ctgttgatga attgacgcat ctatgtccac
    21541 gatgccaaca agttgtaaaa tcacttgtat aattgaaatt gtataaagta ctcatacaga
    21601 tgatataaat taaagctctc ttcataatca tgttgtagtt tttgttgaca tgatgaagag
    21661 agtttttttg tgaataaaaa aatgaccaag ttaccggtca tatatgtaaa aaatgtgcga
    21721 tttactaaaa taaaaattat tcaggaatgg tacaaattct ctgaggcata taaatgcgtt
    21781 atagttgcta ttctcaatta tgttcgcgat aattttaagt aaaagtaagc acagatattg
    21841 aatttgatag gagttaattg aatgtatcat aacagtaacg caaactttgt caatggtatc
    21901 actttaaatg tgagagataa gaatgaatta aagccatttt atgaggacat attaggatta
    21961 aatattataa atgagacatt aacatcgata caatatgaag taggtcaaaa taatcatgtc
    22021 attacacttg ttgaattaca aaatggacgt gaacctttaa tgtccgaagc gggactgttt
    22081 catatcgcaa ttaaactacc tcaaattagt gatttagcta atttactaat tcatttaagc
    22141 gaatatgata ttccagttaa cggaggtata cagcctgctt cgttatcatt attttttgaa
    22201 gacccggaag gaaacggttt taaattttat gttgataaag acgaagcgca atggacgagg
    22261 caaaataatt tagtaaaaat tgatattaga ccattaaatg taccgagatt agtgagtcat
    22321 gcaacaaaat tgttatggtt aggtattcca gatgacgcta ttataggtgc attgcatatt
    22381 aagacaattc atttatcaga ggtaaaagag tactacctcg attattttgg attagagcaa
    22441 tcggcatata tggatgatta ttcaatattt ttagcatcga atggctatta tcaacatttg
    22501 gccatgaatg attgggtatc agcaacgaaa cgtgtagaaa attttgatac gtatggatta
    22561 gcaattgttg actttcatta tcctgaaaca acacatttaa atttacaagg tccggatggt
    22621 atctattatc gctttaatca tatcgaagtt gaagattagt atatactttg aatggacgaa
    22681 ccatataatg aatcgttttt aatgatcttt ttatacaagt tatgaaggag gctgggacat
    22741 taagttctta ggcaatgtaa aaagctgatt tctattaatt atttgataga aatcagcttt
    22801 tttgatatgt attttataat gtacagctcg ttgagctgct attttcctta tattaattgc
    22861 cattaataca aaacctagct ctcgtttaac tttatttatt cctcgaactg acattcgagt
    22921 gaaactcaaa atagcctact ttct
//
LOCUS       DAKTGS010000030        22414 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.030, whole genome shotgun sequence.
ACCESSION   DAKTGS010000030 DAKTGS010000000
VERSION     DAKTGS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.030"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..1157
                     /gene="scpA"
                     /locus_tag="O0286_000990"
     CDS             <1..1157
                     /gene="scpA"
                     /locus_tag="O0286_000990"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCY0169375.1"
                     /translation="FPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSV
                     PTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEG
                     NIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNH
                     KAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINK
                     LENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLT
                     PREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAV
                     VGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1188..1514
                     /locus_tag="O0286_000991"
     CDS             1188..1514
                     /locus_tag="O0286_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCY0169376.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(1806..1979)
                     /locus_tag="O0286_000992"
     CDS             complement(1806..1979)
                     /locus_tag="O0286_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCY0169377.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(1960..2562)
                     /locus_tag="O0286_000993"
     CDS             complement(1960..2562)
                     /locus_tag="O0286_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCY0169378.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(2833..3654)
                     /gene="nadE"
                     /locus_tag="O0286_000994"
     CDS             complement(2833..3654)
                     /gene="nadE"
                     /locus_tag="O0286_000994"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCY0169379.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(3647..5116)
                     /locus_tag="O0286_000995"
     CDS             complement(3647..5116)
                     /locus_tag="O0286_000995"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCY0169380.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            5300..6376
                     /locus_tag="O0286_000996"
     CDS             5300..6376
                     /locus_tag="O0286_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCY0169381.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            6396..7190
                     /locus_tag="O0286_000997"
     CDS             6396..7190
                     /locus_tag="O0286_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCY0169382.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            7262..7369
                     /locus_tag="O0286_000998"
     CDS             7262..7369
                     /locus_tag="O0286_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169383.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(7380..8942)
                     /locus_tag="O0286_000999"
     CDS             complement(7380..8942)
                     /locus_tag="O0286_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCY0169384.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(9147..10244)
                     /locus_tag="O0286_001000"
     CDS             complement(9147..10244)
                     /locus_tag="O0286_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCY0169385.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            10616..11176
                     /locus_tag="O0286_001001"
     CDS             10616..11176
                     /locus_tag="O0286_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0169386.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQSNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            11229..12158
                     /locus_tag="O0286_001002"
     CDS             11229..12158
                     /locus_tag="O0286_001002"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCY0169387.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(12353..12526)
                     /locus_tag="O0286_001003"
     CDS             complement(12353..12526)
                     /locus_tag="O0286_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169388.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            12578..13957
                     /locus_tag="O0286_001004"
     CDS             12578..13957
                     /locus_tag="O0286_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCY0169389.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(14077..15105)
                     /locus_tag="O0286_001005"
     CDS             complement(14077..15105)
                     /locus_tag="O0286_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCY0169390.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            15375..15776
                     /locus_tag="O0286_001006"
     CDS             15375..15776
                     /locus_tag="O0286_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCY0169391.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(15794..16002)
                     /locus_tag="O0286_001007"
                     /pseudo
     CDS             complement(15794..16002)
                     /locus_tag="O0286_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16349..16522)
                     /locus_tag="O0286_001008"
     CDS             complement(16349..16522)
                     /locus_tag="O0286_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169392.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            16973..18013
                     /locus_tag="O0286_001009"
     CDS             16973..18013
                     /locus_tag="O0286_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0169393.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(18070..18912)
                     /locus_tag="O0286_001010"
     CDS             complement(18070..18912)
                     /locus_tag="O0286_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCY0169394.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(18909..19466)
                     /locus_tag="O0286_001011"
     CDS             complement(18909..19466)
                     /locus_tag="O0286_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCY0169395.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            complement(19526..20050)
                     /locus_tag="O0286_001012"
     CDS             complement(19526..20050)
                     /locus_tag="O0286_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169396.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            20171..20734
                     /locus_tag="O0286_001013"
     CDS             20171..20734
                     /locus_tag="O0286_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0169397.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            complement(20800..21540)
                     /gene="pmtD"
                     /locus_tag="O0286_001014"
     CDS             complement(20800..21540)
                     /gene="pmtD"
                     /locus_tag="O0286_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCY0169398.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(21540..22412)
                     /gene="pmtC"
                     /locus_tag="O0286_001015"
     CDS             complement(21540..22412)
                     /gene="pmtC"
                     /locus_tag="O0286_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCY0169399.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
BASE COUNT         7704 a         3873 c         3181 g         7656 t
ORIGIN      
        1 actttccaaa attaatagct ttaagtttaa tattttcttt aagtgttacc cctattgcaa
       61 acgctgagag caattcaaat atcaaagcaa aagataagaa gcatgtgcaa gttaatgtcg
      121 aggacaagag tgttcctact gacgtacgta atttagcgca aaaagactat ctttcttatg
      181 ttacttcact tgataaaatc tataataaag agaaagcctc gtatacactt ggtgaacctt
      241 ttaaaattta taaattcaac aaaaagagtg atggtaatta ttattttcca gttttgaaca
      301 ctgaaggtaa tatcgattat atagttacaa tttcaccaaa aatcactaag tattctagtt
      361 catcttctaa atacacaatc aatgtttctc catttttatc taaagtctta aatcaatata
      421 aagatcaaca aattactatt ttgacaaact caaaaggtta ctatgtggtt actcaaaatc
      481 ataaagctaa actagtacta aaaacaccac gtctagaaga taaaaagtta aaaaagactg
      541 agagtatacc tactggcaat aatgtaacgc aactaaagca aaaagcttct gtcactatgc
      601 caactagtca atttaaaagt aataattata cttacaacga gcaatatata aataaacttg
      661 agaatttcaa gataagagaa actcaaggta acaatggttg gtgtgcaggc tatacgatgt
      721 ctgaattact aaatgcgact tataatacta acaaatatca tgcagaagct gtgatgagat
      781 ttttacaccc aaacttacaa ggacaacgat tccaatttac tggtttaacg ccaagagaga
      841 tgatttattt tggacaaact cagggaagaa gtccacaact tcttaataga atgactacat
      901 ataatgaagt tgacaatctt actaaaaata ataaaggaat tgctgtttta ggtagtagag
      961 tagaatcaag aaatggtatg cacgcagggc atgccatggc agttgttggg aacgctaaat
     1021 tagataatgg acaagaggtt ataattatat ggaatccatg ggataatggt tttatgactc
     1081 aagatgctaa aaacaatgtt attccagtat ccaatggcga ccattatcga tggtattcat
     1141 ctatttatgg ttattaaaat ttgtatttta gaaaggtaaa taatattatg gaacaaattg
     1201 aattatttag tattgataaa ttcaaatgta attcagaggc taagtattat cttaatatta
     1261 ttgagggaga atggcatcct caagatttaa atgatagtcc tttaaaattt attctcagta
     1321 cctcagacga ttctgattac atttgcaaat atataaatac agaacacaaa caactcacat
     1381 tatataataa aaataatagc tcaattgtta ttgaaatatt tataccaaat gataataaaa
     1441 tactactaac aattatgaac acagaagctt tgggaacttc tcctagaatg actttcatta
     1501 agcataagtc ataagactat ctagatatga ttaagtaaac tattaatctt attaaaggtc
     1561 tctaggaaga aaaaatttca tgcgatgaga ttgttttaga aaaagcgacg atatatataa
     1621 tctaacaata atttttaaaa tattaagaac ccccagcata tgctttatat agcttgctgg
     1681 gggtttctca tacaatatgg atcgagcatc ttgcatgtat aaacaaatag gctattcaaa
     1741 gtaaaattac tcaaactttg aatagccttt cctcttcaaa tagtctttat ttaaattgga
     1801 ttatgttatt ggattttctt acctacaaaa acgatttctt gatgtgatac ttcaaccgtg
     1861 ccatcttttt gctcttgaaa cacgggtttt tcaatacgtt ttaccggcat atacccttgc
     1921 attttcattc gtgacaaaca atcagcaatt gtttcattct catttacttt gaatttcatg
     1981 ttctactaca ctttccagtt cttctggttc ataatcttta agctttctac gacgaatgcc
     2041 tttagtccag aatccaccat gtatgtttcg aggttcatac gcaatgataa atgctttcgg
     2101 atctaaattt tttatcgtat ccatcaattt acgttcatat tttcttggtg ttaaaatttg
     2161 catcaccata cgactaccat ctctaccaaa cgcagcatag tgcgtaacgc catatcctaa
     2221 atttcgaagt tcattcggta aatctaactc atattctgct gaagttacat ttacaactgt
     2281 ataacctaat gccagttttt cttctatttt cataccaaca atgataccta ttgaaaaacc
     2341 aaatgcgtag gcaataatat tttgaatatg gtctaaatta gacataacca aacctaaacc
     2401 aacgatataa actaatactt ctaaaaaact aactgatgca gcaatataac gataaccttt
     2461 caacgttaaa attgttcgca tcgttaaaaa cgttacataa caaatgttaa tgataaatat
     2521 agttagtacc attaaccatg gattttctgt tacgaatgac ataaggcgcc ccactctctt
     2581 tcaattacac tttaaatacc aaactgcttc aacctaacct taatgaatgc atattaatat
     2641 aatatagtac gaaaactctc ataaataaat agcgaatttg tcataaataa aaatttattc
     2701 tcaaatgaac atacttactt tagttcatac actttcaatt tatacactga agtagtctag
     2761 ctgaatgtgg tttaatgtta ttaattctaa ctcatattta atttaagtca cacgttagaa
     2821 gaaaaaatta aattaggatt ttggccacgt gtatcttgta tatgcaagtt cacgtttgtg
     2881 tgcatttcgt atataatgat tttcaattac tttttgttct tctggcgtaa ctggcttacc
     2941 ttctaaataa ttatcaatcg cctcataagt tacacctaat gcatcttcat ctggaagctg
     3001 tggtttatca tcttctaaat cagcagttgg cgttttttca tataattcct ttggcgcacc
     3061 aagatacgct aataattgac gaccttgtcg tttattcaaa ccaaatatag gtgcgatatc
     3121 tgcagcacca tcaccgtact tcgtataaaa cccagttata ttttcagctg aatgatctgt
     3181 tcctactaca ataccttgtc ggtttgaagc aattgaaaat tgtactttca tacgttcacg
     3241 cgctttttca tttccttttt ggaaatctgt aagaacaata ccggcttctt ttaatgattg
     3301 cacactttga tcaactgcag gcttaatatt gactgttact atttcatctg gttcaatgaa
     3361 tcgcaaagct tgctcaactt catcagcatc tttttgaact ccataaggta atttaactgc
     3421 aataaacgta caatcaatgc cttcttcacg taattcgtta acagacattt gtactagttt
     3481 tccaactaat gtagaatcct gtcctcctga aatacctaac actaaagatt ttataaatga
     3541 atgtgattgt acataatttt ttataaattg ctttaattcc ataatttctt cagcactatc
     3601 gatacgcttt ttcactttca tttcttgtac aataacgtct tgtaatttac tcattatctt
     3661 cttccatctc cttaacgtgt tccgcaactt caaaaatacg tttatgttta ttatcccaac
     3721 atgccttgct taaatcgact ggatattctt gtggattcag gaaacgctta ttttcatccc
     3781 aaatagattg taatcctagt gctaaatatt cacgtgattc atcttctgtt ggcatttgat
     3841 atactaattt accattttca taaatattat gatgcaaatc aatggcttcg aaagatttta
     3901 taaatttcat tttataagta tgcactggat ggaataattt taaaggttgt tcatcgtatg
     3961 gattttcatt ttccaaagta atataatcgc cttctgcctt acctgttttc ttgtttataa
     4021 tgcgatatac atttttctta cctggcgtcg taaccttttc agcgttattt gataatttaa
     4081 tacgatcact atatgaacca tcttcatttt caatagctac aagtttatat actgcaccta
     4141 atgctggttg atcgtatcct gtaatcagct ttgtaccaac gccccaagaa tctacttttg
     4201 caccttgtgc tttcaaactc gtaatcgttt cttcatccaa atcattagac gcgataattt
     4261 tagtttcagt aaatcctgct tcatcaagca tacgtcttgc ctctttagat aaataagcga
     4321 tatctccaga atctaatcga atacctacaa agttaatttt gtcacctaat tcttttgcaa
     4381 cttttattgc atttggcacg ccagatttta aagtatggaa tgtatctact aggaacacac
     4441 aatttttatg tctttcagca tattttttga aggcaacata ttcgtctcca taagtttgga
     4501 caaatgcatg tgcatgtgta ccagacacag gtataccaaa taatttcccc gccctaacat
     4561 tacttgtaga atcaaagccc ccgatgtaag cagctctagc gccccacaat gctgcatcaa
     4621 tttcttgcgc acgacgtgta ccaaactcca ttaatttatc atttgatgca atttgacgaa
     4681 ttctgctagc ctttgttgta attaatgtat ggaaatttac aatgtttaat aaaattgttt
     4741 ctattaattg cgcttgaatc aatggtgctt ctacgcgtaa caatggttcg ttaccaaagc
     4801 ataattcgcc ttcttgcatc gaacggatgc tgcctgtgaa ttttaaatct tttaaatatg
     4861 ataagaaatc atccttgtag ccaatagact ttaaatattc caaatcagat tctgaaaatc
     4921 caaaatgttc tataaaatca atgacgcgtt ttaaaccatt aaaaacagca tagccactat
     4981 taaatggcat ttttctaaaa tacaaatcaa atacagccat tttttcatga atattatcat
     5041 tccaataact ttcagccata tttatttgat ataagtcatt atgtaacatt aaactgtcgt
     5101 cttctaattg gtacacttgt atctctccaa tcgacctaaa tattttctta cattttatca
     5161 taattcattt ttttatatac ataagagccc cttaatttcc atacttttaa ttaaaatcaa
     5221 ccaacaattt aatgacatat acataatttt taagagtatt ttaataatgt agactataat
     5281 ataaagcgag gtgttgttaa tgttatttaa agaggctcaa gctttcatag aaaacatgta
     5341 taaagagtgt cattatgaaa cgcaaattat caataaacgt ttacatgaca ttgaactaga
     5401 aataaaagaa actgggacat atacacatac agaagaagaa cttatttatg gtgctaaaat
     5461 ggcttggcgt aattcaaatc gttgcattgg tcgtttattt tgggattcgt taaatgtcat
     5521 tgatgcaaga gatgttactg acgaagcatc gttcttatca tcaattactt atcatattac
     5581 acaggctaca aatgaaggta aattaaagcc gtatattact atatatgctc caaaggatgg
     5641 acctaaaatt ttcaacaatc aattaattcg ctatgctggc tatgacaatt gtggtgatcc
     5701 tgctgaaaaa gaagttacac gcttagcaaa tcacttaggt tggaaaggaa aaggtactaa
     5761 ttttgacgtg ttaccactga tttaccaatt acctaatgag tcagttaaat tttacgaata
     5821 tcctacttca ttaattaaag aagtacctat tgaacataat cattatccaa aattaagaaa
     5881 attgaactta aaatggtatg cagtccctat catttccaat atggacttaa aaatcggtgg
     5941 cattgtatat ccaactgcac cctttaacgg ttggtatatg gtaactgaaa ttggcgtacg
     6001 taactttatt gatgattacc gttacaattt actagaaaaa gttgcagatg cgtttgaatt
     6061 tgatacactt aaaaataatt catttaataa agatcgagca cttgttgaat tgaactatgc
     6121 tgtgtatcat tcctttaaaa aagaaggcgt atcaattgtc gatcatttga ccgctgcaaa
     6181 gcaattcgaa ctattcgaac gtaacgaagc acaacaaggt cgtcaagtta ccggaaaatg
     6241 gtcttggcta gcaccgccat tatctccaac attgacgtca aattatcatc acggatatga
     6301 caatacagta aaagatccaa actttttcta taaaaagaaa gaatcaaatg ctaaccagtg
     6361 ccctttccat cattaagtta gtagaggtgt agcatatgca attatattat ttaggcccta
     6421 aaggtacatt ttcatatttg gcttgtcgtc agtatttttc tgaaaatgaa gcaacttttc
     6481 aaccaaagtc aaattttttt gaagtcatca aggcagttgc agatgatgac acatcgattg
     6541 gtgttgtccc tattgaaaat tctatagaag gaacaatcaa cattgtggca gatgcacttg
     6601 cacagcaaga tgtctttgca cacggtgaaa tacgtttaga tattaacttt gcattatatg
     6661 gtaatggaac agattccatt tctgatatta aaaaagtgta ttctattgca ccagcaatca
     6721 gtcaaacaac aaattacata catcaacatc aatttgacta tgattatgtc gacagtacaa
     6781 ttcaaagttt gactaaaatt gaaaatggcg ttgcagcaat tgcaccttta ggtagtggcg
     6841 aagcttatgg ttttacacct attgatacac atattgaaga ctatccacat aacgtgacac
     6901 gttttttagt tataaaaaat cagcagcaat ttgatcaaaa tgcgacatca ttgatgtttt
     6961 taattacacc aatgcatgat aaacctgggt tgttagcaag tgtgcttaat acatttgctt
     7021 tgtttaatat taatttatcc tggattgagt ctcgtccttt aaaaacacaa ttaggtatgt
     7081 atcgattttt cgttcaggct gattctgcaa ttacaaccga tataaagaaa gtcattgcta
     7141 ttttagaaac cttagatttc aaagtagaaa tgattggtgc ttttaattaa actttatttt
     7201 ctaggttcta caatttaaat aaaataaaga gtcaaccaag gattaaaata ggaatccatc
     7261 cttggttgac tttcttttgt ttgtattgtt tttacacgat gctgcggtat acgtttcgtt
     7321 accactcgtc caagcaattg ctaatgtaac cttttatttt tcgttctaat ctgcaattac
     7381 tatttcaatg gcagtggttg atttatatca atacctaaaa ctataggcat tacataatac
     7441 acgactaaaa taataattat tgcactgatt aagtttaccc agaatcctac tgatgccatt
     7501 tgtttgatag atattttacc agaaccaaag ataattgcat tcggtggtgt ccctactggt
     7561 aacatgtatg cacagttagc cgccatagct gcaggtgcca taagtagtaa tggatgcact
     7621 ccaacagcaa cagacaacgt tgctaaaatc ggtaaaatca tcgttgcagt tgcagtatta
     7681 gatgtcactt cagttaaaaa taagacaaag attgttatga caattacaat aagaatcgga
     7741 ctaacaccat ttaatgattt caactgttcg cctaaccatt ttgctaaacc actttcagaa
     7801 atacctttcg ctaatgctaa accgccacca aataaaatta atacacccca agggagctct
     7861 tttgcaactt cccagtcaat gatacggcga tgtttttcag tatttttagc tggaataata
     7921 aataataata ttgatataaa aatagcaatc gtaccatctg caacagatga cgtaacttcc
     7981 cattttttca gaagaaactc tcttgtaatc cataataagc tagcaagtac aaagatagtt
     8041 tgtactacct tttcttcata cttcattttg ccaagctcgt ctaacttttg tttaattaac
     8101 gtctgaccac caggtaaata tttcaaatca tgtctaaacg caacatatct taaatagagc
     8161 caagtaatac ctaacaaaac aatgaccgtt ggaatcccta caatcatcca tttagcaaaa
     8221 ctaatttcat atccaaaatg ttgcatgtat tgtcctttta aaataattaa tggcggggtt
     8281 ccgattaatg tacccaagcc accaatcgta cctgcatagc caattgctaa aactagagat
     8341 ttttcaaact tttgaatact tgtttgattc gtattggctt cttgtaaatc atgtgcttcc
     8401 ttaataattg ctaaaccaat cggaatcata atcattacag ctgcagtgtt cgatacaaac
     8461 atagataaga atcctgttgc caccatgaat ccaagtaata tttttgaagt gcttgcacca
     8521 atcaaattaa taattgtcaa cgctactctc gtatgtaaat tccatctttc cattgcaatt
     8581 gccaaaataa atccacctaa aaacaaaaag ataatatcat tgccatattc ggatgatact
     8641 tgttctggtg taagtatatg acctaatggt aataacacaa ttggtaataa gctcgttgct
     8701 gcaataggaa ttgcttcagt aatccaccaa gtcgcaatcc ataaagtaat cgctaaaaca
     8761 tagacgcctt tccaaggtaa gtcttgtgga tgaaagaata ataatgttaa taggaaaagt
     8821 aatggaccta atattagtcc aataagttgt cccgcactaa acggtttctt tttctttgat
     8881 tttgaaaaga atgttaaata ccttttcgat atcattgatt gatgttgatt gaaataagcc
     8941 ataaatttcc ccctcatgat gattatgaca agctacttca actcactata ttagagtata
     9001 gaaagttgtc tatcttgtat tatcattacc gtcaaaatac cccttttatc agataattgg
     9061 gtttattata gcatatttat accgttttat gaatgcgctt tcaaaaatta tctactattc
     9121 ttaatcctaa tgtcatgcga aactttttac ttttttattt catctacatt gattgtagta
     9181 acttgaatac ccgcttcaac aggacttaaa aacactgtat caatatcttc taaatgaatt
     9241 gattgagtcg aattattgaa tgtattccca acgattaata catctttatg tttaacatta
     9301 ttataattac gtacatgtat cgcatcttta gacattgatc ctttaaattc atttccaatt
     9361 atattcatat ttatgcctgc ttgggaacct aagtcttttc ctgtcatcac atctgctgca
     9421 tttttaccat ctctaactcc taaatatcta atgccaccct cacagttaat aaacttatta
     9481 ttaataatga aagcatcttt atacttcaag ggagttaatg cataaccttg tatatcttca
     9541 aatatattat ttctaatatg aatattctca tagtatcgat tatgtctact tgcatgtgag
     9601 ccaatagcac gattccaact tcccatttcg ggcaattctg aaggtccaaa ataacaatct
     9661 tcgataatga catttttcgt tatcgtacca tctgtcgttc cgaattttgg aaaagcacca
     9721 ggtacttgaa tgtctaattg tattgcttca gaataaaagc gttcgccact atagtctatg
     9781 aatccttcaa atgaacagct tttaatatag agtccgttaa tcccacaagc atcaattgca
     9841 tgaccactca ctacattctt aatggtcaca cctattaatt gaatatcttc agcatgccca
     9901 atgcacatag ctgtattgtt ataaggatac gatacaccat tcatatcaaa cttgccgcct
     9961 ttaatatgaa tatgactgtg tccattataa ccacgataaa aaccaaagcg acgaccattt
    10021 tttaataaag gaccagaatg acatcggcgt aaaatagttt cattatctaa caaaagtgtt
    10081 gtattgccat atatcgttaa tggtttgcaa atatcatacg tccctttcgg tatataaact
    10141 gtcgttggtt tacatcttcc acgatttaac gcacgttgta ttgctcgagt atctttagtt
    10201 ttgtttatgc ctgttaaccc ataatctttc atattgatat acataaatcg tcatcctttg
    10261 tctattttct aattactcat atattaacca attattcaaa ttataatcgt gttaggttga
    10321 tttaacagat agtcataaat ctcgtgatgc catctattag catttttata ttcaaaacat
    10381 caacacttga tattagttct aagtttgtca catttagtat tctattacag ctattttaaa
    10441 actatcgcaa aaacttctta tattatttgt tgattgcgtg atttgctctt ccaaaaaagt
    10501 tgattaatct atatacttaa tctatattta tatattaacc attagggtta aaaattactc
    10561 tagcatttat gaatagatgg gagtttattt tattattata taggagagat gttgaatgac
    10621 acatcgcgca ctattagttg ttgactattc atatgacttt atcgcagacg acggcttact
    10681 aacatgcggt aaacctggac aaaatattga agattttatt gtttctcgta tcaatgactt
    10741 taattattat caagaccata tattcttttt aatggattta cattatttac atgacattca
    10801 tcatcctgaa agtaaattat tcccaccaca caatatcgta gatacaagtg gtagagaatt
    10861 atacggtaaa gtaggtaaat tatacgaaac aattaaagcg caatctaatg tacatttcat
    10921 tgataaaacg cgctatgatt cgttctttgg taccccgctt gatagtttat tgagagaaag
    10981 aagtattaat caagtcgaaa tcgttggtgt atgtaccgat atttgcgtgt tacatacagc
    11041 aatttctgca tacaacttag gttataaaat ttcagtacct gctgagggag tggcttcatt
    11101 taatcaaaaa gggcatgaat gggcacttgc acatttcaaa aactcattag gtgcagaggt
    11161 agaacaacac gtttaaatcg tgctaaaata attataaaga atacaattta caagggagat
    11221 atttgacaat ggctaaaaca tatattttcg gacataagaa tccagacact gatgcaattt
    11281 catctgcgat tattatggca gaatttgaac aacttcgagg taattcagga gccaaagcat
    11341 accgtttagg tgatgtgagt gcagaaactc aattcgcgtt agatacattt aatgtacctg
    11401 ctccggaatt attaacagat gatttagatg gtcaagatgt tatcttagtt gatcataacg
    11461 aattccaaca aagttctgat acgattgcct ctgctacaat taagcatgta attgatcatc
    11521 acagaattgc aaatttcgaa actgctggtc ctttatgtta tcgtgctgaa ccagttggtt
    11581 gtacagctac aattttatac aaaatgttta gagaacgtgg ctttgaaatt aaacctgaaa
    11641 ttgccggttt aatgttatca gcaattatct cagatagctt acttttcaaa tcaccaacat
    11701 gtacacaaca agatgttaaa gcagctgaag aattaaaaga tattgctaaa gttgatattc
    11761 aaaagtacgg cttagatatg ttaaaagcag gtgcttcaac aactgataaa tcagttgaat
    11821 tcttattaaa catggatgct aaatcattta ctatgggtga ctatgtgact cgtattgcac
    11881 aagttaatgc tgttgacctt gacgaagtgt taaatcgtaa agaagattta gaaaaagaaa
    11941 tgttagctgt aagtgcacaa gaaaaatatg acttatttgt acttgttgtt actgacatca
    12001 ttaatagtga ttctaaaatt ttagttgtag gtgctgaaaa agataaagtt ggcgaagcat
    12061 tcaatgttca attagaagat gacatggcct tcttatctgg tgtcgtttct cgaaaaaaac
    12121 aaatcgtacc tcaaatcact gaagcattaa caaaataata ctatattact gtctaattat
    12181 agacatgttg tatttaacta acagttcatt aaagtagaat ttatttcact ttcaatgaac
    12241 tgttttttat ttacgtttga ctaatttaca accctttttc aatagtagtt tttattcctt
    12301 tagctaccct aacccacaga ttagtgattt ctatacaatt ccccttttgt ctttacattt
    12361 tcttaaaata tttgcgatgt tgagtataaa tttttgtttt cttcctacct ttttcgttat
    12421 gattaaagtt ataaatatta ttatgtacac gattcatcgc tctattttca actttcaaca
    12481 tatataattc gaaagaccat ttaaaattaa cggccacaac attcaaatca attaatcgct
    12541 ttttccaaaa taatcatata aggaggttct tttcattatg aatatcattg agcaaaaatt
    12601 ttatgacagt aaagcttttt tcaatacaca acaaactaaa gatattagtt ttagaaaaga
    12661 gcaattaaag aagttaagca aagctattaa atcatacgag agcgatattt tagaagcact
    12721 atatacagat ttaggaaaaa ataaagtcga agcttatgct actgaaattg gcataacttt
    12781 gaaaagtatc aaaattgccc gtaaggaact taaaaactgg actaaaacaa aaaatgtaga
    12841 cacaccttta tatttatttc caacaaaaag ctatatcaaa aaagaacctt atggaacagt
    12901 tttgatcatt gcaccattta actatccttt tcaactagtg ttcgaacctt taatcggtgc
    12961 tattgcagca ggtaatacgg caattattaa accatctgag ttgacgccta atgttgcacg
    13021 agtgattaaa cggttaatca atgaaacatt tgatgcaaat tacattgaag ttattgaggg
    13081 aggaattgaa gaaacgcaaa cgttaattca cttacctttt gactatgtct tctttacagg
    13141 aagtgaaaat gtaggcaaaa tcgtttatca agctgccagc gaaaatttag ttcctgtgac
    13201 attagaaatg ggcggtaaat ctccagtcat cgttgatgaa acagcgaata ttaaagttgc
    13261 tagtgagcgc atttgttttg ggaaattcac taatgccggc caaacatgtg ttgcaccaga
    13321 ttacatttta gtacacgaat ctgtaaaaga tgatttaatc acagccctat caaaaacgtt
    13381 gcgtgaattt tatggtcaaa atatacaaca aagtccagat tatggccgca ttgtaaacct
    13441 taaacattat catcgtctga cttcattact taacagtgca caaatgaata ttgtatttgg
    13501 tggtcatagt gatgaggatg aacgttatat agaaccaaca ttgttagatc acgttacaag
    13561 tgattcagca attatgcaag aagaaatttt tggtcctatc ttaccgattt taacgtatca
    13621 gtcattggat gaagcaatag cctttattca ccaaagacca aaacctttga gtttatattt
    13681 atttagcgaa gatgaaaatg ctacacaacg tgtaataaac gagctatcat ttggcggcgg
    13741 cgctattaat gatacattga tgcacctagc gaatcctaaa ttaccatttg gtggtgttgg
    13801 cgcctcaggt atgggacgct atcatggtaa atattcattc gacactttta cacatgaaaa
    13861 aagctacatt ttcaaatcta cacgattaga atcaggtgtc catttaccac catataaagg
    13921 taaatttaaa tacatcaaag ctttctttaa aaattaattt ttgagacaaa aataaaccat
    13981 gcttccataa tttgatgttc ttgaaaacaa taaatttcaa ggtatacaca aattttttgg
    14041 aaacatggtt ttctttttat catgattaat taaagattaa aatatgacac atacaccttc
    14101 agatgctaca cgagtgttat cacatagcgt aattttacct gtttctttat ttctttcgaa
    14161 aacagttaca actgaatctc cttgctcatg agcacaaact aaaaggtcat ctgatgaggc
    14221 aatattaaaa tctcttggga attgaccacc actttcagta attgttacta gttctaagtg
    14281 ttgaccatta tcaagaactt taaaaattgc aatgctatca tgccctctat tagatacata
    14341 taagaattgt tgatcatgag ataaacgcac tgctgcaagt ttagtatctc catcaaagtt
    14401 ttcaggaatt gttaaatgac gctcgagctc ttcaaattta ccgtcattat attctgcaac
    14461 actcacagta tttgataatt cgtgtacgac ataagcaaat tttccattat catgaaattc
    14521 aatatgtctt gtcccatcac tatctttaaa taaagattct ttataaaatt caaacccgtt
    14581 gtcatcaaat ttataagtaa cgatacgatc agcacctaaa tctgttactg caacgtactt
    14641 accatctgga gtttgattaa tataatgtgc gtgtggatga tcttgtcttt catgtgtacc
    14701 tgttggaaaa tcatgtgcaa gttcttgaat tagacgtata atttcacctg tgtgcgtatt
    14761 taattcatac atacgtatga tgccagcacc atataccgct tcaaataaat atcgtttatc
    14821 ttctgaaatc gatacataac aacctgtacc agcttttgaa gacaaacatt tatttaataa
    14881 atgcaattca ccattgtcat caattttcaa acttgcaaca ccgcattgtt ctccttcttt
    14941 gttgattcca tataaaactt cattattacg caccaaatat gtagacgctt ctaattcaaa
    15001 tcctgtttct aataaatcaa tacgtgactg attttcgttt aattcaaaac gataaatccc
    15061 tttaccattc tttttagtgt aagaaccaat atatccattt gtcattactt ttacccctcc
    15121 atgttagtct tattttcatt ttattaaatt ttagctagaa ttaacaatca tttatcattt
    15181 ccttaaagct aaaactattt aactacttta aaagaactat ttgctgtgat atgtcattat
    15241 tcgattttaa ttaatccctc ttttttactt acacgagcat taattattaa cgcgtttatg
    15301 cttccacaaa atttttgttg caaaatagaa cacatgttcg tataatgtaa ttatcagatt
    15361 ggggtgaatt aaggatgaca agttatgaag aagaaactga ttatcgtaac gttccaagag
    15421 aattactaga taacaatata cctatgggaa gaggcatgat taaatgggct ccttttgcaa
    15481 cattacccga acaattcgaa acgattcaac aatacattat cgaccaaaac aaaattacac
    15541 gtcctgtatt atcagatgat caattggctg agctaaacat tcggctacac gaggcgctac
    15601 aatatgccca acctgtagaa gttaaatttt ataataatgg ctttgtagat tcagtacgct
    15661 taaccattta tcgtattgat gctattaatt atgaaattga tggttatgtt tacaatcagc
    15721 aacaacgtca aaaaatttct atctttgata tcttagatat tgtcttgtta ccttaatgct
    15781 ttgaacataa ttaaacagat tgagtatatg catataagat gatttttaac atcatttttg
    15841 gatgatagga tgttgcgcca cgatgatgtc tgaattcatc gaattcgcta tcaggtattg
    15901 tttcaacaat ttcattaaca tatcgtgaaa tatcattttg agggattcta acagaagttt
    15961 ctattggtag tgtagttggg gcatgttata atttttatac ataaggcacc tcgttaattt
    16021 agtttagtgg tatttattaa attataagaa gggacccaac acagaaaatt cattttattg
    16081 aattttacat ttatgtgcaa gttgggcaaa gtgtcttatt ttttaaaagt atttcaaagt
    16141 aaaattacat gttaatacgt agtattaatg gcgagactcc tgagggagca gtgccagtcg
    16201 aagaccaagg ctgagacggc accctaggaa agcgaagcca ttcaatacga agtattgtat
    16261 aaatagagaa cagcagtaag atattttcta attgaaaatt atcttactgc tgttttttag
    16321 ggatttatgt cccagcctct aaatgttatt agttattttt actttcaata tcagaaataa
    16381 tttctttagt ttttctttca atatcttcaa aatctttttt aaagatataa gcaaatgcta
    16441 ctacaccaac accagcaact aatgttgtaa tagcaactaa gctaaatacg aatttaaaca
    16501 cacatttaat aaaattccac atctcaattt cacctctgtt aaattaaata cctatctaca
    16561 tttcattata ctatcatata tagaataatc attaaataaa tacccacttt cacgaaatat
    16621 attcatcaaa ttatatagtg agcaagctaa tgattatttt caaaaatgcc gaggaacctc
    16681 tgtttcaacg tgacatgacc ttatatgcta atgttaacaa tgactattat cgtgaatgct
    16741 aacttaagat tgatatttat gataagatga ttttaagttt ttaactcaat atccccacca
    16801 tctataaaaa tatgttttaa ctgattgata agtcttatac accgagaaat cacctcaaac
    16861 aattttatat ttcatttagt aaaatataac tttatttaac atgacattca atatacatac
    16921 gattactatt tatttacaat atattacaac taaaaagaaa ggggccgatg atatgcaaca
    16981 agtgacatca gatatcatga cttttagagg ttcacatttc gagttaggtg ttaaaactgg
    17041 taaatggctt caacaaacac ctcttctaaa aaatcgagaa aaagaatgga aaaaacgtgt
    17101 cccacgtttc gatatagatg taaatgaaac ttatcaaata ttccaaacat acgcacctca
    17161 aatatgggaa gaattaatgg gactacaaag tatactcaaa atgccaacac gccaaattat
    17221 tttaaacttt ggacattatc gttttaccga tttaaaagaa agtggttgca cggtctttca
    17281 aggcaaagac ttcatggtta gaaattatga ttaccaccct gcaacttatg atggtcgcta
    17341 cttactttat caacctacag atagcggttt agctcaaatt ggtcctgtat caagggtaac
    17401 tggaagaatg gatggtatga atgagtcagg attaactatg ggctataact ttatgcaccg
    17461 gaagaaacca gcaaatggtt ttgtatgtta tatgattggt cgtctaattc ttgaaaattg
    17521 ccgaaatgta acagaagcaa tccaattatt gaaagaaata ccacacagaa gctcatttag
    17581 ctacatactg atggacaaat cattgaacca tgcaattgtt gaagtgacac cgagatcaat
    17641 agatgttcgt tatgataata tatgtacaaa tcattttgaa atattaacac atgaaaatcg
    17701 taattataca aaagaatcta aagaaagact ggcgcgtacc atttcacaaa ctaatgataa
    17761 tttagatatg actacagcat ttaagttatt caataatcct cagtatgaaa tttacagtaa
    17821 actattcaaa agctggtctg gcaccatcca tacatcaatg tatcaacctg aaacattaac
    17881 tgcatatttt acattgggag aaaatgcgcc accagaaata atagatttca aatcatggtt
    17941 agatggtcaa gaccttaata tcactcactt tactggcaat atagataccg acttaacttt
    18001 tgccaacaaa taaataacac cgatattaga gaaattatgc ttcctaatat cggtgcttac
    18061 atcctttatt tattcaactt tgatattacc atcaatcgta taaaattcta aaacattgtc
    18121 gcttttccca actttaccat tagtaaatgt tttattttta actacgctat cacccgttcc
    18181 cggatttagc tttagtatag tgtcttcagg tttactatca tacttaaaac gaatatctcc
    18241 ttgtttagta gaagcttttg catctacttt tgatggcatg tttttaaatt caatgtcacc
    18301 atgatcgttt aaaaatatgg attgtttaat ttgactcttg tcgatttgta gcgtaccaat
    18361 aacaacttta atattgctat ttttaatgtt acttttacta atataagtag aactattatt
    18421 tgtctttgaa tctagtgcgt caagatttga gtttttaata actacttcac cgttaatgct
    18481 ttgaatactt gtgttctcta aatcaacatc actgattcta acacttcctg ctccagatga
    18541 tagatttaaa cgtttaatca ttttatcagg catttcaatc gttaacgttt tcttattctc
    18601 atgaaaagga ttcatatcaa ttgcatatcc tcttgtctta gaccttttat cactaatctt
    18661 caacgtctta tctactatat taattaatat atcattgtca ccatcatatt taactctaaa
    18721 ttgattcccc cgttttatac gaagttctgt actatccaaa tttatagata cattgtcaaa
    18781 agcatcgtct ttgaatgttt tatcatattg tttagtacca tatttgtttt tatcatagct
    18841 gaaccaaata atggttgctg ctgtaaaaaa gacaacaaac actaaaagcc caataaaaaa
    18901 gaatttcttc attttctaac gcttcctttg acagttttaa tataccaaag taaatatttt
    18961 aagattaaac gataaatgta ttctaaaatc ttaaatatga caatgataaa tactaatcct
    19021 aatcctgaat atgatatggc aaaaaataca ttactaattg agtccacaat tcctctggat
    19081 attgctgatg ctaataaaat aattggacaa aacagcagca aaagtgatat taaaaataat
    19141 gctaatacaa ataatccaac tatagatact ggtataagga tgacaaaaat agatagaatg
    19201 cctaaactta atgaagcagc aacagctctt gaaatatttt caaatgttgg tttagattca
    19261 gcataatcaa tgacacttct agcttttgtt tcagctgcaa catcttgtgg gtcttttaac
    19321 ttctttaaaa tttccccttc atcaacgcca ttaagttctt gctcataaaa ataacgttca
    19381 tattcataca ttttttgatc tcttaaatgc ctaggtagcg catcaagtgc tgtttctaat
    19441 tcatttaaat aagtaatctt gttcatatag gtcactcatc ccaataaaat tattcattcg
    19501 ttttttataa ctttaagacc taaagctatt tactaaaaaa ttcaatacgt ttaatttcta
    19561 aatcttgata aagtaagttt aattttaaat ggttatctct atcaatatac tttaccgatg
    19621 agaaatcatt gccaactttt gttcttttat atccgaatcc aagattttca atcgcgttat
    19681 ttatcggcga accaattccc ccatcaaaat tagtatgtat cttttcatct cttactaata
    19741 ttatcccctt aacgatgtta tccttttctc tcactttaac ggtaagatca gggtgagctt
    19801 tactgttata aaatttaaaa cgatcaaact taattttttt atttacttca tagccttttt
    19861 tatcaaactt ctcatttaat ttaatatcgt ttatttcaag attagacaaa tccacagttt
    19921 tcgtatgacg atattgatat aaaccaatta atacaccaaa agaacagatt aaaaataata
    19981 taaacacaac ccagcgattt tgaatacgca ctgatgtcac cctttctttg tacttattta
    20041 aatatagcat aaggtgcttc ccgtttcatc cttaacaaaa attaacaaag aatttgcaat
    20101 taatttattt cattaccttt tattactata aattggtata atgttgtaaa tgctaaagga
    20161 ggtttttaca atgacaaatt tagaaactta tttcaaaaat agccaaccct taaatgaata
    20221 cattgatgga atgaagacaa atcaagatac cgttctcagt atctatcaat catttgagtt
    20281 accagaagat gattcacgca tcaaaaaaat aaaagagatg aattattcta aagtattagt
    20341 aattactgaa gactggtgtg gagatgcaat gatgaatctc ccaattctaa aacacatcag
    20401 tgaagcatta aatttagaag tacgcgtgtt ccatagagat gatgatacaa aactgatcga
    20461 tcaatattta acaaatggta aatcacgtgc aattcctatc ttcgtattct tgaatgatca
    20521 attcgaacaa gaaacagttt ggggaccaag agcaaatgaa gtccaaaaat ttgtaactga
    20581 tgttcgtgct gataaattac cgagcaaaga tcatccagac tatgcagatt tagaaaaaga
    20641 aacacactta gttatatcaa accgctataa aactgacgct tcattttgga aagcagttta
    20701 taattccatc ctaaataaat tagaaaataa ataaatttac aataagacaa actttgaggt
    20761 tgggaaaatt taaaattccc aacctcaaaa ttatttttat taaatgtctt ttttagcgac
    20821 atataagata ttcaaaatga agaatatcac tatagaacct aatgaaatta cccattgcca
    20881 attgctaaaa tcaaagttag agctaatcat tttatcagtt aagtaagcaa aagggatata
    20941 atcaaaagca tctaaaactt tttcaccgta tcttgggata aatgtaataa aaggtttaat
    21001 aaacggtaca atgagtacta aaaatacacc taatgaaaat atcattgctg ttttttgtga
    21061 tgcaagtgtg attaagaata aaaacaagcc aaagaatagt aaaaagatta ggaagaacca
    21121 taaattatct acatatttag aaaacgccaa attttttcca ttactagatg cctgcacaac
    21181 aatcaaaatc acataatgaa ttaatgcaaa taatattgaa attaaaataa ttgaaattgt
    21241 tttcgaaata atgtatccaa cgcgtgtttt aactttactc atatatagtt gaattgtacc
    21301 ttgtgaaaaa tcacgcgtaa ttgtcttaac aacaaacatt aatccaatta ttaacaataa
    21361 ccattttgct gcagcaaata cagatccata gacaataata tgtttcgggt tatccatcga
    21421 atttgccata agtatacttt gagttgcaat caaactagat acgactagta tcgctaaata
    21481 tgttaaagga cttttaaata tactataaaa atcatactta actaaattta aaattctcat
    21541 tatttatcac ctctttgatt tatattgaag tacgtatcac gcaatgaact tttacgtgtt
    21601 tcaatatatt tcggataaat atctaattca gataatgctt ttagtataac ttgataattt
    21661 ttttgtgcat tgatcattaa atgtccgtct gctttgtcga cattttgtaa ttggaaatgc
    21721 tctgcaagat atttttcagt tctatcaaag tctttatgat caacagtaac tatcgttgtg
    21781 tcagatgcaa caccttcctc catgtttaca tcttgaacaa aatgtccgtc tcttaaaaat
    21841 acagctctat cacaaattaa ttcaatatct tctaacttat gacttgatat tagaattctc
    21901 atatcaagtt catttactaa agacttaatt gtagtcagca catcaattga gccatctgga
    21961 tccataccat ttgtaggctc atccaagatt aaaaatttag gtttattcat taaagatact
    22021 gcaattgcta acttttgctt catccccatt gaatatttct taactttctt tttaatataa
    22081 ggtctcatac caaatgcatc tataattttg tctgtgtatg ctttatcaaa acccttacct
    22141 aatacttgtg caaataattt caagttatac aatcctgatt tattatcata taatttagga
    22201 tgctcaatta aaaaaccgat attatctgca ttatcaatat ctacttttcc atcaaattta
    22261 ataatattac cattcattac tttcataacg gttgttttac caacaccgtt ttttcctatt
    22321 aatccgacaa ttctactatc gccaaagtca aaatcaatat catttaaaac gacatttgag
    22381 ccgtattttt ttgtaatatg ttctaatttc atcg
//
LOCUS       DAKTGS010000031        22056 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.031, whole genome shotgun sequence.
ACCESSION   DAKTGS010000031 DAKTGS010000000
VERSION     DAKTGS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.031"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            142..954
                     /gene="lpl8"
                     /locus_tag="O0286_001016"
     CDS             142..954
                     /gene="lpl8"
                     /locus_tag="O0286_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCY0169400.1"
                     /translation="MGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFPDRKNYKVEMKNNKIILLDKVEDTNLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPAKVDEDDE"
     gene            973..1758
                     /gene="lpl9"
                     /locus_tag="O0286_001017"
     CDS             973..1758
                     /gene="lpl9"
                     /locus_tag="O0286_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HCY0169401.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
     gene            1941..2753
                     /gene="lpl10"
                     /locus_tag="O0286_001018"
     CDS             1941..2753
                     /gene="lpl10"
                     /locus_tag="O0286_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCY0169402.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
     gene            2743..4071
                     /locus_tag="O0286_001019"
     CDS             2743..4071
                     /locus_tag="O0286_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169403.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            4068..4391
                     /locus_tag="O0286_001020"
     CDS             4068..4391
                     /locus_tag="O0286_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169404.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            4410..4724
                     /locus_tag="O0286_001021"
     CDS             4410..4724
                     /locus_tag="O0286_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169405.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(5014..5274)
                     /locus_tag="O0286_001022"
     CDS             complement(5014..5274)
                     /locus_tag="O0286_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169406.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            5393..6595
                     /locus_tag="O0286_001023"
     CDS             5393..6595
                     /locus_tag="O0286_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0169407.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(7254..7316)
                     /locus_tag="O0286_001024"
     CDS             complement(7254..7316)
                     /locus_tag="O0286_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCY0169408.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(7380..7448)
                     /locus_tag="O0286_001025"
     CDS             complement(7380..7448)
                     /locus_tag="O0286_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCY0169409.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(7500..7565)
                     /locus_tag="O0286_001026"
     CDS             complement(7500..7565)
                     /locus_tag="O0286_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCY0169410.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(7597..7662)
                     /locus_tag="O0286_001027"
     CDS             complement(7597..7662)
                     /locus_tag="O0286_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCY0169411.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            8183..9667
                     /locus_tag="O0286_001028"
     CDS             8183..9667
                     /locus_tag="O0286_001028"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCY0169412.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            9680..12385
                     /locus_tag="O0286_001029"
     CDS             9680..12385
                     /locus_tag="O0286_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCY0169413.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            12547..12909
                     /locus_tag="O0286_001030"
     CDS             12547..12909
                     /locus_tag="O0286_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0169414.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(13138..13554)
                     /locus_tag="O0286_001031"
     CDS             complement(13138..13554)
                     /locus_tag="O0286_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169415.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            13592..14266
                     /locus_tag="O0286_001032"
     CDS             13592..14266
                     /locus_tag="O0286_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0169416.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            14303..15037
                     /locus_tag="O0286_001033"
     CDS             14303..15037
                     /locus_tag="O0286_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0169417.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            15215..15328
                     /locus_tag="O0286_001034"
     CDS             15215..15328
                     /locus_tag="O0286_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169418.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            15493..16830
                     /locus_tag="O0286_001035"
     CDS             15493..16830
                     /locus_tag="O0286_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCY0169419.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            17047..17961
                     /locus_tag="O0286_001036"
     CDS             17047..17961
                     /locus_tag="O0286_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCY0169420.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            17945..19087
                     /locus_tag="O0286_001037"
     CDS             17945..19087
                     /locus_tag="O0286_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCY0169421.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            19381..20406
                     /locus_tag="O0286_001038"
     CDS             19381..20406
                     /locus_tag="O0286_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0169422.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            20410..21069
                     /locus_tag="O0286_001039"
     CDS             20410..21069
                     /locus_tag="O0286_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169423.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            21106..21948
                     /gene="gmpC"
                     /locus_tag="O0286_001040"
     CDS             21106..21948
                     /gene="gmpC"
                     /locus_tag="O0286_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCY0169424.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
BASE COUNT         7698 a         3104 c         4367 g         6887 t
ORIGIN      
        1 aataaagtta aattaacaaa ctgtattaaa ttcatatagt cagttttgtt tgatgacatg
       61 atagtagaaa aattattatt ttaataggtt gtatgaacta tacaaatatt tataaaaatt
      121 aaaacagaga gatgtgaaat gatggggtat ataaaaagaa tggctttata catgagtgta
      181 tttcttttaa tcatttttat tgttggatgt cgaaatatga aagatgaaca gaaaaaagaa
      241 gaacaaacaa ataaaacaga ttcaaaagaa gaacaaatca aaaagagttt tgagaaaaca
      301 ttagatatgt atccaattaa gaatctcgag gagttatacg acaaagaagg ataccgagat
      361 ggcgaattta aaaagggtga taaagggatg tggacgatat atacagattt cgccaaaagt
      421 aataaacaag gtggattgag taatgaaggt atggtcttat acttagatag aaatacacgg
      481 actgcaaagg gacattattt tgttaagaca ttctataata agggcaaatt cccagataga
      541 aaaaattata aagttgaaat gaaaaataat aaaattatct tattagataa agtagaagat
      601 acaaatctaa aaaagagaat agaaaacttt aaattttttg gacaatatgc aaaccttaaa
      661 gaattgaaaa actacaacaa tggtgatgtc tcaattaatg agaatgttcc aagttatgac
      721 gcaaaattta aaatgagcaa taaagatgaa aatgttaagc aattaagaag tcgttataat
      781 attcctactg ataaagcacc ggtattaaaa atgcatattg atggtaattt gaaaggaagt
      841 tctgtgggtt ataaaaagtt ggaaattgac ttttcaaaag gtggaaaaag cgatttgtca
      901 gtaatagatt ctttgaattt ccagccggcg aaggtagatg aagatgatga atgatgagga
      961 tggtgtgtaa caatgaagtc tataaaaagg attggattgt gcattagttt gttgatttta
     1021 atcatctttg ttacatcttg tgatggtgat aataagatca ttggagattc aaaagaagaa
     1081 caaatcaaaa agagctttgc gaaaacgtta gatatctacc ctattaagaa tctcgaggat
     1141 ttatacgaca aagaaggata tcgagatggc gaatttaaaa aagatgataa aggtacttgg
     1201 ctaattagat ctgaaatgaa aatccaatta aaaggagaaa atctggaatc tagaggagca
     1261 gttttagaaa ttaacagaaa tactagaacg gctaaagggc attatattgt tagagaagtt
     1321 gttgaagata gcgatggaat gacacacaat catacaaaaa gatatcctgt gaaaatggaa
     1381 aataataaaa tgattccatt aaaaccaatc gatgacgaaa aagtaaaaaa agaaatcgaa
     1441 gaatttaact tctttgtaca atacgggaat ttcaaagaat tggaaaacta taaagaggac
     1501 gaagtgtcat ataacccaga agtaccaatt tactctgcaa aatatcaatt gaaaaacagt
     1561 gattacaatg ttgaacaatt aaggaagcga tataatatcc cgacgcaaaa agcgcccaaa
     1621 ttattattga aaggctcagg taatttaaaa ggttcatcag tcggatataa aaatattgaa
     1681 tttaccttta ttgaaaacaa ggaagaaaat atttacttca cagatagtat ctactttaat
     1741 ccaagcgagg ataaataaat ataattacta atataaaaaa taacgttaga acatttaata
     1801 ataaagttaa aatgaattac atgtattaaa ttgaataagt tagttttgtt tgataacata
     1861 aaagttgaat aatcactatt ttaataagtt gtatgaaata ttcaaatgtt tataaaatta
     1921 aaacagagag atgtgaaatg atggagtata taaaaaaaat tgctttgtac atgagtgtat
     1981 tacttttaat catttttatt gggggatgtg gaaatatgaa agatgaacag aaaaaagagg
     2041 aacaaacgaa taaaacagat tcaaaagaag aacaaatcaa aaagagtttt gcgaaaacgt
     2101 tagatatgta tccaattaag aatctcgagg atttatacga caaagaagga tatcgagatg
     2161 gtgagtttaa aaaaggcgat aaaggtacgt ggactatact tacaggtttt tcaaaaagta
     2221 acaaaccagg agtattagat gatgaaggca tggtgttata tcttaataga aatgccaaaa
     2281 aggcaacagg ttattatttt gtaaataaag tttatgatga tattagcaaa aatcataatg
     2341 agaaaaaata tcgtgttgaa ctaaaaaata ataagattgt tcttttggat aatgtagaag
     2401 acaaaaaact taaacaaaaa attgaaaatt ttaaattttt tagtcagtat gctgatttta
     2461 aagatttaaa aaattatcaa gatggaaata taacaactaa tgaaaacgta ccgagctatg
     2521 aagcacaata taaaatgaac aatagtgata aaaatgtaaa aaaacttaga gaaatttatc
     2581 caattacaac taataactct ccaaatctaa aattatatat agatggtgat ataaaaggaa
     2641 gctcagtagg atataaaaaa atagaatata aattttcaaa agataaaggt caagagacaa
     2701 cattaagaga ttatttgaat tttggaccgt ctgaaggtga gaatgttgag taggaagtat
     2761 aaaatagatt taaaagcaat taatcaaaat actgagtcta caagtgctat ttcgaaagcg
     2821 tcatacgaag ttgagaatgc taataataat ggtctatcaa aaagagacgt gattaatcaa
     2881 tttaatgatt tgaaaaaaat gaaaaaattc ccttctaatc ttgaatatgt tgatagttac
     2941 actgattctc ttactggagt aacaacttct gcttttttaa ataaagatac aggcaaagta
     3001 actctcggga tgactgggac taatttacaa gacgaagcct ttaaaaagtt aaaagaaggt
     3061 gaattttcaa gacaaaatgt taccaatgct ttggaaacag ttaaagatgg atatgcagat
     3121 cttaaaatat tatattctcc tgcatctgat caaaactata gatatgcgaa tacacaagaa
     3181 tttataaata aaataaaaag taagtatgac attgatttta ttactggaca ttcactaggt
     3241 ggaagagatg cggtagttct aggaatgagt aatggtattc cgaacattgt ggtttataat
     3301 ccagctccta tttctataac tagtttgaat cctaattccc cagatggaaa acgtttatta
     3361 gaattatata aaaattataa aggtaatatt actaggtttg ttgcagaaaa tgatgcattg
     3421 acagaaaatc tgaagaaata taagcattat gtttttttcg gtaatgataa agtctttaaa
     3481 aatggtaaag gtcatgaaat ggaaggcttt ctgaccgaag aagaacaaaa agctataaaa
     3541 aaagaactta aaaaactaca aggttatgca gaagaaaata ataagtcatt tgtaaagaat
     3601 tcaaataatg ctatctctaa attagctagt atagaattac ttagagcaaa tatgatgact
     3661 acaaatggag gaggattatc ttcttcgcag caaaaagttt tagaaagttt aacagcttta
     3721 acaattgcgc agtcattcag tcaactgata gacgatgaaa ttaatcaaat caaaaaaatg
     3781 tataatgaaa agaaaaagaa atttggaaaa aattgggaag acgctcaaaa agctggaaaa
     3841 gctgtaggtg aagatttgag tgtaaatgga gttctcaatg ctttagatga aggtcaagtg
     3901 aatgaaagta gtatggtaag agaacctgaa caaatgatat ctgcaaaaga aagacaactt
     3961 tcaacgatag ggtcttctgt atcaaattat attatgagag ttagactcag tattaatgaa
     4021 atcgttgata aagatcaagt gcttgcatca caaataggtg gattactatg atgtttaatc
     4081 aaattaataa taaaaatgaa ttagaagaat catatgaatc tgagaaaaaa cgtatagaga
     4141 atgaactgca aaatttaaat gaacttaggc atagaactcg aaaagaaaat gagcgtagtt
     4201 atgatgtttt tcaatatttg aagcacgaaa tgaattatag tgaagatgcc caaaggaaaa
     4261 tgacgagaaa tatagaagcg tatgagcaag aaatcaatga gataattaga aagcaagaat
     4321 ggaaattaga agaatataaa gaagacttaa aaaagtctta tgaaaagcag ttagataagc
     4381 taagtgactg ataatttgga ggagattaaa tgactctaat agaaccagat atgaccttaa
     4441 gaatgccaga tataagtact acagtagaaa cacttaatct catatctaaa atggaagcgc
     4501 aaaaagaaaa tattcgcaca gttattgcac ctgaacataa gcataaatac aaagatattg
     4561 aaaacggatt aaaaggtgaa gaaaaagtat taattgaaca aatggcgcaa cattgcgaag
     4621 cttttaaagc taattttaaa ggtgcagctc aaggagattg ggttaaaagt gccatgtctg
     4681 agatagacag cattaaggat gacctgaaaa aaattaatag ctaaactaca tatcatacta
     4741 ctaaataaag cgaccaatgt tcagtatatt cacaactgac actaggccgc ttctttttaa
     4801 tttatattat tcccatgtaa agtaattcga accgaatcga aatatcacac tctcaattgt
     4861 aaaaaaacaa cttaaattac tgtcccgcat taaacatgac cacattcgta acagcaacaa
     4921 taaaaatcct caagtactaa aagctatgat cactttatca aaatcaacgc aagttaacct
     4981 ctcccgaact acccgttact catcaccttc accttacaaa ccataaagcg ctgcagttaa
     5041 gtattgaatg gcatagccaa ttgaaatgaa aatagcaatc gttataatag cactgccaat
     5101 ggtatataaa gtatgtttcg tagataatcc ttcagtcttt gaaccaaaaa cactgagtag
     5161 aataaatgca ataccagtaa catataaaac taaaatgaca aataacatgt ttatacacct
     5221 cgattctctt tcgtgagata tatctttgtc tccattataa agtaaagcgc tcaataattt
     5281 aattaagtat actatttttt tattatcgaa gattgcgaaa gaggaaacgc tcctatataa
     5341 tgtaaaacgg aattattcct atttacaaat aaattgaagt gagggtatat atatggctaa
     5401 aattccagtt acggtattaa gtggttattt aggctcgggg aagacaacgt tgttaaatca
     5461 tattttacaa aatcgagaag gtcgacgtat cgcggtaatt gtaaatgata tgagtgaagt
     5521 aaatatcgat aaagatcttg tcgcagatgg tgggggacta tcgcgtacag atgaaaaatt
     5581 agtcgaactt tctaatggtt gtatctgttg tacacttaga gacgatttat taaaagaagt
     5641 tgagcgttta gtgaaaaaag gtggcatcga tcaaattgtt attgagtcaa cagggatttc
     5701 agagccagta cctgttgcac aaactttctc atatattgat gatgaacttg gcattgatct
     5761 tacagcgatt tgccgtttag atacaatggt tacagttgtg gatgctaacc gcttcgtaca
     5821 tgacatcaac tcagaagatt tattgatgga tcgtgatcaa agcgttgacg aaacagatga
     5881 gcgttcgatt gctgatttat taattgacca agttgaattt tgtgatgtat tgattattaa
     5941 taaaattgat ttaattagtg aagaagaact agcgaagtta gaaaaaatgt taagcgcatt
     6001 gcaaccgact gctaaaatta ttaagacaac aaattctgaa gtagatttaa aagaagtctt
     6061 gaatacgcag cgttttgatt ttgaaaaagc gagcgagtca gcaggatgga tcaaagaact
     6121 tgagtctggt gggcatgcat cgcatacacc tgaaacagaa gaatatggta tatcatcgtt
     6181 tgtatataaa cgtcgtctac ctttccatgc taaaaggttc aatgattggt tagaaagcat
     6241 gccaaataat gtcgttcgat caaaaggtat cgtatggcta gcacaataca atcacgtagc
     6301 atgtttatta tctcaagcag ggtcatcttg caatattcat ccagttacat attgggtggc
     6361 tagtatgtct gaagcgcaac aaacacaaat attagcagaa cgtcaagatg tcgcagctga
     6421 atgggatcca gaatatggcg atcgtcatac acaatttgtc attattggta cagaattaga
     6481 tgaagaaaaa ttaacaaaag aactcgacgc atgcttagtc aatgcgcaag aaattgatgc
     6541 agattggcaa caatttgaag atccatatca atggcaaatt agaccagcac gataagttga
     6601 atgaagtata atcatttttg aattgtggct taattgtttg caatttatga caattaataa
     6661 aaagttaagg tgttcttagg atctttttgt tttcaatcta tctaaagtgt gatagttttg
     6721 ataaagcaga aatttgcatt ttatccatag ggctaggaca tgtatgtgtc ttagtccttt
     6781 ttatatttac gttgatgaaa aatggcaaaa ccatcgtacc aactgttaat tgaaggtaca
     6841 atcattgttt aacagtcata aaaatgagtg aagtaaatgc agctatcaat gtactattaa
     6901 cacttacggt cacacgagtg tttcagtcta aacgtgcaac gcaattccgc acgtggccaa
     6961 tatatcttgc gctagatcat gaaaatcatt aagatttcgc atgccaaatc atgaatgtct
     7021 cagaactcga gtatcaaaca taaaacacgc cacaaaaata taacgcgtca gactacaaag
     7081 atcacgaata ttccgtatgt cccactaaga acgtcctata caaaaaacac caagaccatt
     7141 tatttgcaac aaactaacaa gactcacctc acatcaataa atcaacacaa agcaaagcca
     7201 ccatccctat tggtatagtg gcctgagaat tttaagtatt caattcgctt aaattatttt
     7261 gcgaaaatgt cgataattgc tttgatgatt ttaatgatag tacctacaat agccatcgtt
     7321 ttgtcctcct gtatgttgat ttaacatagt gatgagttgt tgatcgttaa tgtttgagat
     7381 tagttgttac ctaaaaattt accaagtaaa tctttaaaga atttgaataa ttttgctacg
     7441 aattccatgt gaatggcccc cttcaaataa gatgttcata tctatgagat ttttataact
     7501 tacttaccag tgaatttctc aattaatcct ttaatgaatt taatgattcc tgcaatgata
     7561 cccatgtgaa agacctcctt tgtttgttat gaaatcttat ttaccagtga attgttcgat
     7621 taagcttttg ataactttaa tgatgccagc gatgataccc attaagatta cctcctttgc
     7681 ttatgagtta acttcattgt acatagttat cttgtgcgta attgattttt ttcctaactt
     7741 gaattgttat gatcataact gcgaattttt tattttaaaa ggacgggaaa tactgcctaa
     7801 ctgtacaggt gcatttacaa aatctttaca atcattggtt taatagaggt taagctcatg
     7861 aatttgacat gaagcagaaa tttaattata ttttatgtta taagtttaaa acgaataaca
     7921 cgttaggtct cgtctaggca aagcatattt atgaagatga attgaacaga cagaagtaac
     7981 attttatata catatgtgca tgggagcacg cttaaagaga acactgcaac aaggtatatc
     8041 aacgtaccgt gatgcagtgt ctagttaaac tgaaacctgc aatagtaaat tttctgccat
     8101 tatgtacaga atctactatt gtaggttttt atttttatac ataagcaatt atgcgagaga
     8161 ttagaaataa ggaggttatg cagtgttaag ttttcaattg ctattttcac tgtttgttat
     8221 tgcgcttatc attgcattga taagtggctt gttgttttta gcaccagtta tgccaatgag
     8281 atatattaaa ttacatttat acatactagt catgccagta ttatttgcag tcattggctt
     8341 tttcggtatt catggtcaac atgtcttagg tccatttaaa atagatcgtt tatcttggtt
     8401 attagctggc tttgtaatgg cgcttggctt tattattcaa aagttttcaa tgcgatattt
     8461 actaggtgat catcattata gacattactt tccattgttc actgcgatta cgtcgtttgc
     8521 atctttagca tggatgtctg aagacttaag actgatggca ctctgctggg gtattacatt
     8581 attatgttta acattgctga tgaacgttaa tcgtttttgg aaagtgccac gtgagtctgc
     8641 gaaattatca agcatgacat ttttatgtgg ttggcttgca ttcgttggag caattgtaac
     8701 tatttatatt gcgactggcg agtggcgggt accacaacat atagttcatc cgacatggtc
     8761 attgttgacg aatgtactac ttgtattagc tgtcatgata ccggcagcac aatttccttt
     8821 tcatcgatgg ttgattgaat ctgtaacggc accaacgcca gtatcggcaa ttatgcatgc
     8881 aggaattgtg aatgcaggtg gtgttattct aactcgtttt gcgccgatat ttgataatgg
     8941 atttgcgtta tcattattac ttatcctttc tagtatttct gtattgttag gatcgggtat
     9001 tagcttagtt caagttgact ataaacgcca attagtgggc tctacgatga gtcaaatggg
     9061 ctttatgtta gttcaatgtg cattgggtgt atattcagca gcgattattc atttaatatt
     9121 gcacggtatt tttaaagcaa cattattttt acaatcaggt tctatcgtga agcgattcaa
     9181 tattccaaaa caagcatctg ctaaagacgc ttatggctgg attgtcatgg gacgtgtatt
     9241 agctattatc gtggcatttc tattttggat gagtagtgac agaagtgcat atgaagtgtt
     9301 aagtgcactc attctaggat ggtcattact tgtatcttgg aatcaaatgg tagcctttag
     9361 taaaggacgc atggcacgtc ttgttggtat gattttgatt gcaatcgtga catttatcta
     9421 cgtcatcaca cataattatt tttacgctgt attacaaaat ataacaacac atgcgacaac
     9481 gccgcctaca gtgagtgtca tcattagtgt tgttatttta atctttggca gtttattaag
     9541 tatttgggtg gcgcggcatc gatactctaa ggcttttgcg gtattgtacg tgtggttagt
     9601 taatctaggt gaagcacgct cgaaagcgat agaaagtcat ccgaattatt tgaagaagta
     9661 tttataggag gtgaaaggta tgacaacaca gttaaatatc aattcagtca ttgaaaatgc
     9721 gaaacgtgtt attacaccat tatcaccaat ttcgattttt gcagcacgaa atccatggga
     9781 aggattagaa gcggatacgt ttgaagatgt cgcaaaatgg ttacgtgatg ttcgagatgt
     9841 ggatattttc ccaaataaag cattaataga aagcgctgtg gcacgtggtg aattagatga
     9901 aagtgtcttt aatcaacttg ttactgatat gttacttgaa catcactaca atatcccgca
     9961 acactacatc aatctttata ttgataacat taaaacatta aaagacgtac ctgcatcata
    10021 tatgaatcat tcaaatgttg atgttgttgc tgatctacta ttagaaaaat caaaacgtga
    10081 tatggctgaa tcatatcatc actatgatgt acgtccgatg agtgatgcaa taatagatga
    10141 acaaggtgag ccacttagcg aacaagtgaa tcgtcaaatg attaaatgga cgaaacttta
    10201 tatcgatcaa tttctatcga gttggacaat gccgaagcgt gagcaaagtt tttaccatgc
    10261 atggttgcat ttagcgcaac atgaccatag ttttactaaa gcacagcgcc aagtgattaa
    10321 aggcttaccc aatgatcctg aaatgacgat agagtcagta ttaactcatt tttcaataga
    10381 tcaggaagac taccaagctt atgttgaagg acatcttttg gcgttaccgg gttgggcagg
    10441 tatgttgtat taccgttcac aacagcatta ctttgaacaa catttgttaa cggattattt
    10501 ggcaattcgg ttagttgtcg aacaattgct agttggtgat gagtttaagt cagtcgctaa
    10561 agattgtgaa agtagatcgg aaaattggtt taagcaaact gttgcatcat ggtgttacta
    10621 cagtgatatg cctagcgatg tattactaca acatgacgtc aatgaaattc aaacgtttat
    10681 tcattttgca gcaactatga ataaaaatgt atttaaaaat ttatggctaa ttgcctggga
    10741 aatgacatac gaatctcagt taaaacaaaa aattaaagca ggtcatgaaa gtgtggcggg
    10801 cgcattagat gtaaaccaag taaatgtctc agaaaatgat aacgctaatc agccacattc
    10861 agtattgtta aatgacacac aagcagttga tgaaaataat agcgagctaa atcagatggg
    10921 cacatcaacg aaagcgcaaa ttgcattttg tatagatgtt cgttcagaac catttcgtag
    10981 acatatcgaa gcagcagggc cctttgaaac gattggtatt gcaggcttct ttggattacc
    11041 tattcaaaaa gatgccgtag acgaacaatt caaacatgat tcattacctg tcatggtacc
    11101 gccggcatat cgcattaaag aatttgcaga ccgctacgat atgaatgttt atcgacaaca
    11161 gcaacagaca atgtcatcga tgttttacac atttaaattg atgaaaaata atgtcatgcc
    11221 tagtctgtta ttgcctgaat taagtgggcc atttttaagc ttaagtacca ttgtcaattc
    11281 gattatgcct agaaaaagtc gcgcgtcttt acaaaaaata aaacaaaagt ggttgaaaaa
    11341 gcctgaaaca aagttgacga ttgatcgtga gtttgaccga acatcagact tacctgttgg
    11401 atttactgag caagagcaaa ttgatttcgc gttacaagcg ttgaaattga tggatttaac
    11461 cgaagcattt gcgccgttcg ttgtgttagc aggtcatgct agtcattctc acaataatcc
    11521 acatcatgca tcacttgaat gtggggcttg tggtggcgca tcaagcggtt ttaatgctaa
    11581 gttattagcg atgatatgta atcgtccaaa tgtcagacaa ggattaaaac aatcaggtgt
    11641 gtatattcca gagacaactg tttttgcggt agcagaacat catacgtcta ctgatacgtt
    11701 ggcatgggta tatgtgccag acacattatc ttctattgct ttagatgcat atgaatcatt
    11761 gaatgacgcg atgccgatga tttctgaaca cgcgaatcgc gaacgtttgg acaaactgcc
    11821 aacgattggt cgtgtgaatc atccagtgga agaagcgcag cggtttgcga gtgattggag
    11881 tgaggtacgt ccagaatggg gattggctaa aaatgcatca tttataattg gacgacgcca
    11941 attaacaaaa ggcattgatt tagaagggcg gacattttta cacaattatg attggcgtaa
    12001 agataaagat ggcacattat taaataccat catttctggt ccggcacttg tggcacaatg
    12061 gattaattta caatattatg cgtcgacagt tgcgccgcat ttttacggaa gtgggaataa
    12121 agcgacacaa accgtcacgt caggtgttgg tgtcatgcaa ggtaatgcga gtgatctgat
    12181 gtatggctta tcatggcaat ctgttatggc tgctgatcgg acgatgtatc attcgccaat
    12241 tcgtttactt gtcgttattc aggcacccga ctatgttgta gcaagactac tcgcgaataa
    12301 tgagcatttc gctaggaagg tgtctaatca ttggctgcgt ttaatgagcg ttaatgagga
    12361 agggcgtttt aaaagttgga tttaacgatg atatgttgcg atgctaagta actaatatat
    12421 catcatatct acaatgttct agcttcttaa aatatactaa aaatgataaa cttaatgtaa
    12481 tagttgatgt cgtgataacg atacaaagat gaagtaatca attttaatat tattggagtt
    12541 agtgatatga aaagaacgaa aggtgaaatc gaagctgaaa tcagtaaagc cattacgcaa
    12601 tgggaaaaag atttccttgg cagaggttcc ttgtcagtta aatcagacat tttaagagat
    12661 atggtgatta ttagtttaca aggtatctta acgccagctg aatatcgtgt atgtagtacg
    12721 aatgaaggat tattaaatat taaacgaaca cgttctgaat tagttgaatc cggtgagcaa
    12781 gatttgaatg atatcatttt taaaattaca ggtatcaaag tgatgagctt ccatagtgat
    12841 ttaagtacag ttacaggtga acgtattatc gtattcaaac ttgaggataa tttggaaaag
    12901 catatttaag aaggttaggt atgcttctgc tggcttatta aagctataga agtatgcttg
    12961 gttaaacagc gcaacgccat atatttgggt gtgctgtttt tctattacat gatgtgttta
    13021 ttacgtggat gaatgcgtgg gtctgaatat agcccgtgaa aggcgtatgt cataaaattt
    13081 gcgaatggtg acaagtcgac gcaagcatat ttgttgcgag acatgcccac tatcctttta
    13141 cataaagagt atatgtgtga cgtaggcata taatcgataa agtattccta aaaaattagg
    13201 tataatgacc attgttgcaa taagtttttc ggccttgaaa ccgataacgc atagtataat
    13261 aggaataaaa tataatacaa atatccagta aatcaaattt gaaaatgatg gatgaatagg
    13321 ggtaaagaat ctaactaatg taatgattaa cgctatatag acaaaaataa ctctattgat
    13381 acgccttacc ccctctgtat aataaatata aatcgtacta aattgaaaat agcaaaatat
    13441 tgttatttca attatacaat gttttatttg caatatacat aacaatatga actttataat
    13501 tttgatattg tcggtaaagt gaaatttgtg gcacattgat attatggata acatttgtaa
    13561 aatgaaaatg atgatacgag gagattgtaa gatgatagat aaaaaattaa catcaccgaa
    13621 aatgacagtg cctttatttt taatcgcgct gattgtattt ataggtatgt tttacagtgt
    13681 agtgacaaat caagaatggc ttaaaaatat agatatggga tcattaacat ggtttacaga
    13741 ttatttcggt gagccacaac gtcagtatgt taacaatttg tttaattact atatgacgtt
    13801 tagtgcggaa attggagatg tcaaaggtgt cgtgttgatt tccattatcg tcacaatcat
    13861 actgtttatt aaacagaggc atttagcggt ttggtttgtg acatatttgg tttcaggtgt
    13921 catcatgaac aaattaatta aagatactgt attacgtcca agaccatata atcatttagc
    13981 cgttgataca ggcttttcat ttccgagtgg acattccaac gccagcacat tattatattt
    14041 cgccttaatg atcattatta tttcacttgc tgctaagaca ataacaaaag tgttgagtgc
    14101 gttggttatg ggaatattat ggcttagcat attattttgt cgcctttatt ttcatgcaca
    14161 ttacttttca gatgtcattg gcggcacgtc actagcaatc atttgggtag cgttattctt
    14221 aatggtatac ccatacttta ttaatcatcg acgacaacgc gtttagcaaa catctttaga
    14281 catagtggag gtatatagaa aaatgagaat taaaacaccg agtccatcgt atttaaaagg
    14341 cacaaatgga catgcgatat tattattaca ttcatttaca ggtacaaatc gggatgtgaa
    14401 gcatcttgca gctgagttaa atgaccaagg atttagttgt tatgcaccga attatccagg
    14461 tcatggttta ttgttgaaag atttcatgac atataatgta gatgattggt gggaagaagt
    14521 tgagaaagct taccaatttt tagtcaatga aggttatgaa tctatcagtg caacgggtgt
    14581 gtctttaggt ggattaatga cattaaaatt ggcgcaacac tatcctttga aacgtatcgc
    14641 tgtcatgtca gcaccaaagg aaaagagtga cgatggctta atagaacatt tagtttatta
    14701 tagtcaacgc atgtcgaata ttttaaattt agatcagcaa gcatcgagtg cgcaattagc
    14761 agcaattgat gattatgaag gtgaaattac gaagtttcaa cattttattg atgatatcat
    14821 gacaaattta aatgttatta aaatgccagc taatatatta tttggtggta aagatgcgcc
    14881 atcctatgaa acaagtgcac attttattta tgaacattta ggatcagtag acaaagaatt
    14941 aaatggtctg aaggattcgc atcatttaat gacgcatgga gaaggcagag atattttaga
    15001 agaaaatgtt attcgctttt tcaatgcttt aacataattg aagattttaa aaattaacac
    15061 ttgagtgacg atgttattat atttgtcatt tgagtgtttt ttgtggcgtg atagtatagg
    15121 gaacttagct aaagttaatt tgtagtgtga tgtcgtggaa atattatgga gttataccag
    15181 aagttgattt gtattgtaat gtcgtggaaa ttttatgggc attgcacctg aagttgttat
    15241 gtggcgagat gttggggata tagatggtgt tgtatgtggc gagatattgg cgcacgtata
    15301 cagcgttgaa ccagaaaatt taatgtagcg taatgacaac acattacaag agttgattat
    15361 attatgataa atccaggtgc taccgtatta atgaaacaat tttgatgcag gtgtattgca
    15421 tgttatttac atacatgtta gagtgttact taaacacgta gacgctttaa cgctttaaag
    15481 gagatgtgaa tgatgaaaag acaacaatca caatggaagt catcaactgg atttatttta
    15541 gctagtgcgg gttctgcaat cggtcttggt gccatgtgga aattcccata tatggcaggg
    15601 atttatggcg gcggtgcctt tctagctatg ttcttaatat tcaccatttt tgttgggttg
    15661 ccattactca ttatggaatt cactgttggg aaaatgggac ggacatatac aacacaaata
    15721 tatagtaaat taactggtaa aaaatggctc aatatcattg gctggaacgg taatttggca
    15781 gtgtttattt tatttggctt ctatagtgtt atcggtggtt ggattgtcat ttacatcgga
    15841 caagtattat ggcaattagt tatatttcaa cgcatcaatc atctccaaga aatgaatttt
    15901 gaagcggtaa tatcaaatcc ttggttaacc gttctagggc aaggtatatt catattcgct
    15961 acgatgatta ttgtcatgtt aggtgttgaa aaaggattag aaaaggcatc gaaagttatg
    16021 atgccattgc tgtttgtctt tttaatcgtc attgtgatta agtctttaac attagatggc
    16081 gccttagaag gtgtgaaatt tattttacaa ccaagagtat cagagattac tgctgatggc
    16141 atcttgtttg cgctaggtca atcattcttt acgttatcat taggaactac aggtatgatt
    16201 acttatgcga gttatgcctc taaagacatg acgattaagt catcagctat ttctatcgtt
    16261 gttatgaata tctttgtatc tgtattggca ggtctagcta tatttccggc tttacatagt
    16321 tttggctatg aaccacaaga agggcctgga ttattattta aagtactgcc aatggtcttt
    16381 agtcaaatgc atctaggcac attattctat ttgggattct tagtgctgtt cttatttgcg
    16441 gctttaacgt catctatttc tttattagaa ttaaatgttt ctaacttcac gaagaatgac
    16501 aatacaaaac gtaaaaaagt cgcagtgatc ggtagtattt tagtatttat cattagtatt
    16561 ccagcaacct tatcttttgg tatcttaaaa gatgtaagat tcggtgcggg aacgattttt
    16621 gataatatgg atttcatcgt ttcgaatgta ttgatgccat taggcgcatt aggtactacg
    16681 cttgtcgtag gacaattatt agataaaaaa ttattacaac aatattttgg taaagatcga
    16741 tttagattat tcagtggttg gtattactta attaagtatg cgatgcctgt cgttattatt
    16801 ttagtcttta tcgtgcaatt atttagttaa tatatagaag catctgacac acaagtattt
    16861 gtgttgctca ggtgtttttt tgtttggaat tgaaaattta gtactggttc tgaattaatc
    16921 gaaacttaga taattgcaaa ataacaaaat gtcgtttata attcttatct gaaaagtaag
    16981 aattaaaatt gagacatgaa gaaaggtttc attcatttga tcggcatgtc agatgagggt
    17041 gcatctatga ttacttatga tttaattggc aatacaccat tagtactgtt agaacattat
    17101 agtgatgata aagttaaaat ttatgccaag cttgaacaat ggaatcctgg aggcagtgtt
    17161 aaagacagac tcgggaaata tttagtagag aaggcaattc aagaagggcg tgtgcgtgca
    17221 ggtcaaacta ttgttgaagc gactgctggt aatacaggca tagggttagc tattgcagcg
    17281 aatagacatc atttgaaatg taagatcttt gcgccgtatg gtttttcaga agaaaagatt
    17341 aatattatga tagcgcttgg tgcagaagtt tcaaggacga gtcagtctga aggtatgcat
    17401 ggggcacaat tagctgcacg ttcctatgct gaaaaatatg gtgccgttta tatgaatcaa
    17461 tttgaatccg aacataatcc ggatacatat tttcatacat tgggacccga attgacttca
    17521 gcattacagc aaattgatta ttttgtggct ggtattggct ctggcggtac atttacaggt
    17581 accgcacgtt atttaaagca acatcacgtg caatgttatg ccgttgagcc agaagggtcc
    17641 gtgttaaatg gagggccagc tcatgcacat gacactgaag gtatcggttc tgagaaatgg
    17701 ccgatatttt tagagagacg tcttgtagat gggatattta cgattaaaga tcaagatgcc
    17761 tttcgaaatg tcaaaagttt ggctataaat gaagggttgt tagtaggcag ttcttcaggt
    17821 gcagcattac aaggtgcatt gaatttaaaa gcgcaattat ctgaaggtac gattgttgtc
    17881 gtatttccag atggtagtga tcgatatatg tctaagcaaa tatttaatta tgaggagaat
    17941 aataatgaac aagaaaacta aattaattca tggtgggcac acaacagacg attatacagg
    18001 tgccgttaca acaccaattt atcaaacaag tacatattta caagatgata ttggtgattt
    18061 acgtcaagga tatgaatatt ctcgtactgc gaatccaaca agaagttctg tagaaagcgt
    18121 tattgcgaca ttagaaaatg gcaaacatgg ctttgcattt agttcaggtg ttgcagcaat
    18181 cagtgcagtt gttatgctgt tggacaaagg agatcatatt attttaaatt cagatgtata
    18241 cggcggtact tatcgcgcat tgacaaaagt atttacacga tttggcattg aagtggattt
    18301 tgtagataca acgcatacag attcaattgt acaagcgata cgcccaacaa caaagatgtt
    18361 gtttattgaa acaccttcta atccattatt acgtgttact gacattaaaa agtctgctga
    18421 aattgcgaaa gaacacggtt tgatttcagt tgttgataac acatttatga caccttatta
    18481 tcagaatcca ttagatttag gtatcgatat tgtcttacat tctgcaacga aatatttagg
    18541 tggacatagt gatgtcgttg ctggtttagt tgcaacatcg gatgacaagc ttgcagaacg
    18601 tttagcattt atttcaaatt caacaggtgg cattttagga cctcaagata gctatttact
    18661 tgtgaggggt attaaaacat taggtttacg tatggaacaa attaatcgca gcgttattga
    18721 aattattaaa atgttacaag cacatccagc tgtgcaacaa gtgttccatc caagtattga
    18781 aagtcattta aatcatgatg tccatatggc tcaagcggat ggccatacag gtgtgattgc
    18841 atttgaagtg aaaaatacag aaagtgccaa acaattgatt aaagcaacat cgtattacac
    18901 attagctgaa agtttaggtg cagtggaaag tttaatttca gtacctgcat tgatgacaca
    18961 tgcatccatt ccagcagata ttcgagctaa agaaggtatt acagacggac ttgtaagaat
    19021 ttctgtaggt attgaagata ctgaagattt agtcgatgat ttaaaacaag cactagatac
    19081 tttataaata atagcagcac tggcatatat tttgagttca acgtttgttg ttcttgaata
    19141 tgctagtgct tttttgtgcg atgttaatat ttagtaattg cgttgtggag gattggtgga
    19201 catttttatg gatttaattt gataaatgtc atagtagtct cacaattcgt cattgtcaca
    19261 tgaattactt attttttaat tttttagaaa attcggcaaa ttgtattgac gatatatgca
    19321 attttacata taatgctctt attcctagtg gattaataag aatttgtagg aggggcgatg
    19381 atgattgagt ttcgacaggt aagtaagacc tttaataaaa agaagcaaaa aatagatgct
    19441 ttgaaggacg tatcatttac ggtcaatcgc aatgatattt ttggtgtgat tggatatagt
    19501 ggtgcaggaa aaagtacgtt ggtaagactc gtgaatcatc ttgaagctgc ctcgaatgga
    19561 caagtgattg tagatggaca tgatattacg aattatagcg ataaaatgat gagggatatt
    19621 aagaaagata tcggtatgat atttcagcat ttcaatttat taaattcagc taccgtattt
    19681 aaaaatgtag caatgccact cattttaagt aagaaaagca aaacagaaat taagcaacga
    19741 gtaacggaaa tgcttgaatt tgtaggattg agtgataaaa aagaccaatt tcctgatgaa
    19801 ttatctggtg ggcagaagca aagggtggct attgcaagag cgcttgttac taatccgaaa
    19861 atactcctat gcgatgaagc aacaagcgca ttggatccag caacgactgc ttcgatattg
    19921 acgttattaa agaatgtcaa tcaaaccttt ggcattacaa ttatgatgat tacacatgaa
    19981 atgcgcgtta ttaaagacat ttgtaatcgt gttgctgtaa tggaaaaggg gaaagtggtt
    20041 gaaacaggaa ctgttaaaga ggtgtttagt catcctaaaa cgacgattgc tcaaaatttt
    20101 gtgtctacag ttatacagac tgagccaagt acatcattga ttcgtcgatt gaatgacgaa
    20161 caagttggcg attttaaaga ttataaaatc ttcgtcgagg aaactcaggt gacacaaccg
    20221 attataaatg acttgattca aatttgtggc agagaggtta aaattttatt ttcatctatg
    20281 tcagaaatac aaggtaacac cgtatgttat atgtggcttc gatttaatat ggatcaacaa
    20341 tttgaagaca cggcaataaa tcaatatttc aaagagaaaa atattcaatt tgaggaggtg
    20401 cattaacata tgtttggttc tgatttagac agtgcacagt tattacaagc attgtacgaa
    20461 acgctatata tggtatctat tgctttattt ttaggagcag tgattggtat tccattaggt
    20521 gtcttattgg taattactcg aaaacaaggc atatggccca atatagtgat acatcaagtt
    20581 ttaaatcctt taatcaatat tttaaggtca ctaccattta ttattttgtt aattgcgatt
    20641 gtgccattca caaaattagt agtaggtact tcaattggta cgactgctgc catcgtgcct
    20701 ttaacagtat atgtggcacc ttacattgca agacttgttg aaaactcatt attggaagta
    20761 gacgagggga ttattgaagc ggcgaaagcg atgggcgctt caccactaca aatcattaga
    20821 tattttttaa ttcctgaagc tttaggttcg ttagtattag caattaccac tgcgattatt
    20881 ggacttattg gaagtacggc gatggcagga gctgttggcg gtggtggtat aggagactta
    20941 gctttagtgt atggttatca aagatttgat acgacggtca ttattattac cgttattgta
    21001 ttagtcatta ttgtccaagt gattcaaacg ctagggaatg ttctagctag attcatacgt
    21061 agacattaat gatatatagt gaagattttg aaaggaattg atagaatgaa aagattgatt
    21121 gggttagtta tcgtagcact tgtattatta gcagcgtgtg gtggtaacaa tgataaaaaa
    21181 gtaacaattg gtgtcgcatc aaatgacact aaggcttggg agaaggttaa agaattagct
    21241 aaaaaagatg atattgatgt ggagattaag cacttctcag attacaattt accgaataaa
    21301 gcattaaatg atggtgatat tgatatgaat gcattccaac attttgcatt tttagatcaa
    21361 tataaaaagg cgcataaagg aacaaagatt tcagcattaa gtacaacagt tttagcaccg
    21421 ttgggcattt actcagataa aattaaagat gtcaaaaagg ttaaagatgg tgctaaagtt
    21481 gtcattccaa atgatgtgtc aaaccaagca cgtgcactta aactattaga agcagctggt
    21541 ttaataaaac tgaaaaaaga tttcggatta gcaggcacgg tgaaagatat aacgtcaaat
    21601 ccaaaacatt taaaaattac tgcagtagat gcacaacaaa ctgcacgtgc tttatctgat
    21661 gtcgatattg cagttattaa taacggtgta gcaactaaag cgggtaaaga tcctaaaaat
    21721 gatccgatat ttttagaaaa atcaaattca gatgcagtaa agccatatat taatattgtt
    21781 gcagttaatg acaaagactt ggataacaaa acatatgcta aaatcgtaga attgtatcat
    21841 tcaaaagaag ctcaaaaagc gttgcaggaa gatgtcaaag atggagagaa acctgttaat
    21901 ttatctaaag atgagattaa ggcaatagaa acgtcattag caaaataaat tatattgcgt
    21961 cctacaagca aagttcatgc ttatgtttgt agggcgttat tgttggagaa taaaattatt
    22021 tccaatagag aaagggattg taatcatttt atagtg
//
LOCUS       DAKTGS010000032        21473 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.032, whole genome shotgun sequence.
ACCESSION   DAKTGS010000032 DAKTGS010000000
VERSION     DAKTGS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.032"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            5..1189
                     /gene="rocD"
                     /locus_tag="O0286_001041"
     CDS             5..1189
                     /gene="rocD"
                     /locus_tag="O0286_001041"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0169425.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            1228..2259
                     /locus_tag="O0286_001042"
     CDS             1228..2259
                     /locus_tag="O0286_001042"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCY0169426.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            2271..3512
                     /gene="argJ"
                     /locus_tag="O0286_001043"
     CDS             2271..3512
                     /gene="argJ"
                     /locus_tag="O0286_001043"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCY0169427.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            3528..4292
                     /gene="argB"
                     /locus_tag="O0286_001044"
     CDS             3528..4292
                     /gene="argB"
                     /locus_tag="O0286_001044"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCY0169428.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            4562..5041
                     /locus_tag="O0286_001045"
     CDS             4562..5041
                     /locus_tag="O0286_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCY0169429.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(5369..6013)
                     /locus_tag="O0286_001046"
     CDS             complement(5369..6013)
                     /locus_tag="O0286_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCY0169430.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(6026..13201)
                     /locus_tag="O0286_001047"
     CDS             complement(6026..13201)
                     /locus_tag="O0286_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCY0169431.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(13648..14898)
                     /locus_tag="O0286_001048"
     CDS             complement(13648..14898)
                     /locus_tag="O0286_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0169432.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(15284..16309)
                     /locus_tag="O0286_001049"
     CDS             complement(15284..16309)
                     /locus_tag="O0286_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCY0169433.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(16482..16841)
                     /locus_tag="O0286_001050"
     CDS             complement(16482..16841)
                     /locus_tag="O0286_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0169434.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(17058..18089)
                     /locus_tag="O0286_001051"
     CDS             complement(17058..18089)
                     /locus_tag="O0286_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0169435.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(18102..18863)
                     /locus_tag="O0286_001052"
     CDS             complement(18102..18863)
                     /locus_tag="O0286_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169436.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(18860..19834)
                     /locus_tag="O0286_001053"
     CDS             complement(18860..19834)
                     /locus_tag="O0286_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0169437.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(19848..20588)
                     /locus_tag="O0286_001054"
     CDS             complement(19848..20588)
                     /locus_tag="O0286_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169438.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(20930..21442)
                     /locus_tag="O0286_001055"
     CDS             complement(20930..21442)
                     /locus_tag="O0286_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCY0169439.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
BASE COUNT         6922 a         4146 c         3310 g         7095 t
ORIGIN      
        1 accgatgaat tcaatcattg aattaactga ttattatagc tctaataatt atgcaccact
       61 taagcttgtc atttctaaag gtaaaggtgt caaagtttgg gatactgatg gcaaacaata
      121 tatagattgc atttcgggtt tttcagttgc aaaccaaggc cattgtcatc caacaattgt
      181 taaagcgatg acagaacaag cttcaaagtt gtctatcatt tcacgtgtcc tttatagtga
      241 caatctcggg aaatgggaag aaaaaatttg tcatcttgct aagaaagaca aagtactccc
      301 ccttaactct ggtactgaag ctgttgaagc agccattaaa attgctagaa aatggggctc
      361 tgaagttaaa ggcattactg acggacaagt tgaaatcatc gctatgaata acaattttca
      421 cggtcgtaca cttggctcat tatcactatc taaccacgac gcatataaag caggatttca
      481 ccccctactt caaggcacta caacagtaga ttttggagac attgaacaat taacacaagc
      541 tatttcaccg aatacagcag caattatttt ggaaccaatt caaggtgaag gtggcgttaa
      601 tataccaccg aaaggatata ttcaagctgt gcgtcaacta tgtgataaac atcaaatatt
      661 attgattgca gatgaaattc aagttggtct tggtagaact gggaaatggt ttgctatgga
      721 atgggagcaa gtcgttccag acatttatat tttaggtaag gcattgggtg gcggcttata
      781 ccctgtatct gctgtacttg caaataatga tgtcatgcgt gttctaacac caggtacaca
      841 tggttcaaca tttggtggta accctttagc cattgcaata tcgacggcag cgcttgatgt
      901 acttaaagat gaacaactgg ttgaacgatc agaacgctta ggttcatttt tattaaaagc
      961 gttgctacaa cttaaacatc ctagtattaa agaaattaga ggtcgtggtt tatttatagg
     1021 catagagctt aacacagatg ctgcaccttt tgtggatcaa ctgattcaac gtggaatctt
     1081 atgcaaagac acgcatcgta ctatcattcg attgtctcca cctctagtca ttgataaaga
     1141 ggaaatccat caaattgttg cagcttttca agacgttttt aaaaattaac aattaatcat
     1201 ttatatatga cataggaggg attcatgatg attaaagtag gtatcgttgg cggtagcggt
     1261 tatggcgcaa ttgaattaat tcgattgtta caaacacatc ctcatgtaac gattgcacac
     1321 atctactcac attcaaaagt agatgaaccg ttgaaattaa catttccaca tttacaacat
     1381 attatgcaac atttcgaagc acttacagtg gacaataatg actgtgatgt aattttcttt
     1441 gcgacaccag cacccgtaag taaaacatgt atccctccct tagtagaaaa aggtattcat
     1501 gttatcgatt tatctggcgc atttagaatt aagaatcgtg aaatatatga agcatattac
     1561 aaagaaactg ctgcagcaca agatgatttg aatcatgcta tttacagcat ttcagaatgg
     1621 caatcgtttg ataacaatgg aacgaagctc atttctaatc ctggctgttt ccctacagca
     1681 acattattag cattacatcc acttattagc gaaaaaatag tagatttgtc atctattatt
     1741 attgatgcta agaccggcgt gtcaggtgct ggtcgttcat tatcacaacg tgttcatttt
     1801 tcagaaatga atgaaaatct aagcgcttat gcaatcggaa accataaaca caaaccggaa
     1861 atcgagcaat atttatctat cattgcgggt caagatgtat cagtcatatt tacaccacat
     1921 ctcgtaccaa tgacacgagg tattttatca acaatatatg tcaaattatc atctgaatat
     1981 acgactgaat cattacataa attaatgacc tcttattatg ctaatcagcc atttgtcaga
     2041 attagagata ttgggacttt tccaaccaca aaagaagtac tcggtagtaa ctactgcgat
     2101 atcggcatct atgtagatga aacaacgcaa acagcaattt tagtatcagt gattgataac
     2161 cttgtcaaag gcgcaagtgg gcaagccatt caaaatttaa atatattata tgattttgaa
     2221 gtgacgactg gcctaaatca atcaccagtt tatccataag gggtgttaga atgaaacatc
     2281 aagaaacgac atcacaacaa tataactttt caattattaa acatggcgat atcagtacac
     2341 ctcaaggctt cacggctggt ggtatgcaca tcggtttacg cgctaacaaa aaagactttg
     2401 gatggattta ctcatcgtct ttggcaagtg cagctgccgt atatacttta aatcagttta
     2461 aagctgcacc acttattgtc actgaagaca ctttacaaaa gtctaaagga aaattacaag
     2521 cacttgttgt taattcagct aatgcaaatt cttgtaccgg tcaacaaggc atagatgatg
     2581 cacgacaaac acaaacatgg gttgctcaac aacttcaaat accatctgag catgttgctg
     2641 ttgcttcaac tggggtcatt ggtgaatatt tgcctatgga taaaattaag actgggaccg
     2701 aacatattaa ggatgctaat tttgcaacgc caggtgcgtt taacgaggca attttaacaa
     2761 ctgatacctg tacaaaacat atcgctgtat cactaaaaat cgatggtaaa accgttacaa
     2821 ttggtggtag caccaaaggt tcaggtatga ttcacccaaa tatggctacc atgcttgctt
     2881 ttataacaac cgatgcatcg attgaatcga atacacttca tcaattatta aaatcttcga
     2941 ctgaccatac atttaatatg attactgttg atggcgatac aagtacaaat gacatggtat
     3001 tagtcatggc aaatcaccaa gttgaacacc aaatacttag tcaagaccat ccacaatggg
     3061 aaacatttgt tgatgcattc aattttgtct gtacattttt agctaaagct atagccagag
     3121 atggcgaagg cgcaacaaag ttaatcagcg tcaatgtgtc aggcgctaaa tcaatatcag
     3181 atgcaagaaa aatcggtaaa actatcgtaa gttcaaatct agtaaaatca gctatttttg
     3241 gcgaagatgc caattttggt cgaatcatta cagctattgg ctacagcgga tgtgaaattg
     3301 atcctaactg cacatatgtt caactgaacc aaatacctgt cgttgataaa ggtatggctg
     3361 tactatttga tgagcaagct atgtcgaata cattaactca tgaaaatgtc acaattgacg
     3421 ttcagcttgg tttaggtaac gctgcagcga ctgcatacgg ttgtgattta tcctatgatt
     3481 atgtgcgtat caacgcatca tatcgaacat aaggtggtgt tggttagatg aaatttattg
     3541 tcattaaaat tggtggcagt acacttagtg acatgcatcc atcaattatt aacaacatta
     3601 agcatttacg atcaaacaac atctacccca ttatcgttca tggcggtggc ccatttatta
     3661 atgaagcatt atcaaaccag caaatcgagc cacactttgt taatggccta agagtgactg
     3721 ataaagcaac catgaccatt actaaacaca cgctcattgc agacgttaac actgcattag
     3781 tagctcaatt taaccagcac caatgttctg caataggctt atgtggtttg gatgcacagc
     3841 tgtttgaaat tacatctttt gatcaacaat atggatatgt cggtgttccg accgctttaa
     3901 ataaggatgc tttacagtat ttatgtacta aatttgtacc tatcatcaat tcgattggtt
     3961 tcaataacca tgatggagaa ttttacaata ttaatgctga cacgcttgcc tattttattg
     4021 catcatcatt aaaagcgcct atttatgtat taagtaatat tgcaggtgta ctcatcaatg
     4081 atgttgttat acctcaattg ccattagtcg atattcatca atatattgaa catggtgata
     4141 tttatggagg tatgattccc aaagtgctag atgccaaaaa tgcgattgaa aatggctgtc
     4201 ctaaagttat cattgcatca ggaaacaagc caaatatcat tgaatctatt tacaataatg
     4261 attttgttgg cacaacaatc cttaattcat aactatgaaa ttaaggccta acaagttttg
     4321 acacgcgaga tgattccagt tcgattatcc attgcgctaa aacatttatt taccgttcat
     4381 ctcgttaaca attttgaata cagtacgata caatatgaga tgtaaaaaac taataacctt
     4441 ttacaaattt gtttatcaaa atattttaag ttttgcaaag ctttttattg tgattatttt
     4501 cacaaaatac tataatgagg atagtaaata gagaggagtc cttaagttga cgaaacgaca
     4561 aatgggtata ttcatttatg ctggaattat cggtggcttg ttatctggaa ttgtaaaatt
     4621 aggttgggag gtcatgtttc cacctcgcac accagaacgt aatgcaacga acccacctca
     4681 agagttattg caacaattag gatttagtag tgagtttacg catcaaacat atacattttc
     4741 aaatatggaa ttgccttggg taagctttat tgtccacttt agtttttcta tcgtcattgc
     4801 aattatttac tgcatattag ttaaaaaata cgcttactta gcaatgggac aaggtgctgt
     4861 ttttggtatt gctatttggg tattattcca ccttatcatt atgccaatca tgcatactgt
     4921 acctgctgtg tgggatcaac cattccaaga gcatctgtca gaattctttg gccatatcgt
     4981 ctggatgatg acaattgaat tagtgcgaca acattttgtc tatcgctata aattaaatta
     5041 atacactgac taacattaac gtgagtttta aatcatcgtt tgagtatgat gattgatgct
     5101 cacgttattt tattaactga catgatatga ttccagccaa cttacgtgag cattaaagtc
     5161 tcaaatgcgt cgtaacaaac tattattttc ggtaatttca atattgctca gtatattttt
     5221 accttatcac ttactttaat ctcgtcatga ttttgaatga tgccatcgtg tattcacctt
     5281 tcatttttcc aataaaaaaa catctaacag taaacattta ggcagtatag tttaaaatca
     5341 ctgcgcaatg atactgtcag acgtcatatt aactactcaa taactgaaat acagacactt
     5401 ttttataacc ccagggtgcc tgtcctaaga aacatacctg taccataaac tgatcaaaaa
     5461 taaattgttt gaacttcact tcacgtgatt gataaaagtg tgattgtgtc atatcataaa
     5521 tgtccaatcc tttgattaaa ccttcaccaa tcaattttgt aaaactttct ttttgtgtcc
     5581 atatttgata aaaatcattt aaactacata tttgatgtgc ttcgtttgta gagaaacacg
     5641 tcactaacgt acgccagtct aaacgttgtg atatcttttc gatatcaata ccaactggtt
     5701 ctttatcgac aacacacacg atataaggat aactatatga taagctcaca tagatgggct
     5761 gtccatcacg attgtgttga acaatatctg ccttacctcg tggcgaaatg tgataatgcc
     5821 attcatgtgg taataaacct gtgtcatgtt gaattccata ttgcactaaa atatctccca
     5881 atctgtgcat gagtttatct tgattgtatc tatagttgac tgtacgcggt tttttatatg
     5941 accaacgact ttgtgatatt aattcttcaa tacttttcaa gttactctgt aattgcatta
     6001 caaatactgt cataactttc cctacttact tattgaatat tgttttgata tattgtgccc
     6061 aatgatacag ccaattgtta gttatcgttg gccatttttc actgatgtga ttcattattt
     6121 ttaatgttaa tgttgtatct atcattgcta gttgttgttc acggtcaaca ctagttaatc
     6181 caatcgtttc gtacatgtct tgtttctgta aaatttcatt aaatgattca tcgctgacga
     6241 gttcaatttc tttgcgctta acgcattcta acaaagattt caccggcatt ttattaggtg
     6301 atagcacatg gtaaatgatt tgtggtgtgt tgacctgtgc taatgcgaca atttgtcttg
     6361 cagtcgtatc cacaaaagaa aaatctacag gcatttcagc catgctaacc ccgatacaat
     6421 ccagttgtaa caaatcattc attaccattg aaaaacggtt agtctttata tttctcatat
     6481 gccatcttcc attgtaagga ttcgtcaaat taccaacacg tacaatccga ccatctaagc
     6541 cattatttac agcttctaat acttttaatt cactataaaa tttgctccgt gtatatggtg
     6601 atgttagtag ttgcccttta tagacatccg cttctgaaaa tgtcacatct tctgtgtcta
     6661 tatcaaaata agttcccaca cttatcgtag acacatatat taaccttgca tgatgttgtt
     6721 gtgccaaacg tatgacatca acagtacctt gaacatttac tttttcaaat tcatcatcat
     6781 caccaaagtg atctgtacga gcacctgcat gaataatcgt atccatgttt tctggtaaaa
     6841 caacatcatc catacactcg aaatcaccaa caatgacttc aatgtttgat aacattattt
     6901 caaccgtctc ttctgaaaaa taatcattta aattcgtcat caacttatac catgctattt
     6961 cctcattatc agcacgtatg aaacaataaa tgcgatgact gtatccttgt agtacttcaa
     7021 tcagataagc acctaaaaaa cctgtcgcgc cagtcaatag tgtatttcct agaggtcgat
     7081 gacttagact atcctctaaa atacccaagt tataacgaga cataacaatc ttttgtaatt
     7141 ccgaaagatt atccggtaat gcaactaatg attgttgatt ttggtacata taattaacaa
     7201 tctgtcgcac ggttttatat tggtataatg tctgcattga aatatgatgg ccaaatcgtt
     7261 ttaaatgcga gacaactaac atcgcctcta atgagttacc accaagttca aagaaatcat
     7321 cgtcaacacc gacatcattt tgtttcaata cctctccaaa tacatcaaca aatgtctgct
     7381 caatttcatt agagggttcg ctatacactt tattagactg ttgtataggt gatggatttg
     7441 gcaaacgcgt agtatccacc ttgtcattcg tggttaatgg catacaatcg atatgcgtta
     7501 tagtcttagg aatcatatac ttaggcagct gatcatttaa atattgcttc aaatcctgtt
     7561 ccacttgttg ctctccgaca taataagcat tcaatatatc atgcgtatca aagtgactta
     7621 ctgttacaac acaatcagat ataccacgaa tagctaatat tgcattttca atttcatcaa
     7681 gttcaatacg gtacccgtta actttcactt gtttatctat tcttcctaaa aattcaattt
     7741 gaccatcaga tgtataacgt gctaaatcac cactatgata caactttcct ttaccaaatg
     7801 gattattttg ccatttatca gccattaatt ctggacgatt aatatatcct atcgctaaac
     7861 tatcacctgc aatacacaac tcgcctggca taccaatacc gcataacaaa ccatctgaca
     7921 taatatacac ttggatgtta gataagggtt tgccaattgg aatcgtctca ggtatcaaat
     7981 caccacaatg atgtgaccaa tacgatgtga tgactgttga ctcagatggt ccataggcat
     8041 tgaaatacgt gccacaatgc ttctcaatat atttaacaaa ggatgccgta ctagttgccc
     8101 cgcctgtaat caacttttca atataaaagt cttccataac actacacatc tgtaacggaa
     8161 tcgacgcaac cgtcacacga tgcttattaa tgagttgttg taactgttct ggattaacac
     8221 gttcctctct atctggaatc acaagcgtat gaccatttaa caaacaacaa taaatctcca
     8281 taactgatgc atcaaaaaca atatttgcat gttgcaaaaa tacttcattg tcgcctaatt
     8341 gcaattcagt tgaccatgca tgcactaaat tcaacaaatt tcgttgtcgt atggcaaccc
     8401 ctttaggcat cccggtcgta ccagatgtgt aaatagcata catctcatta tctaacatcg
     8461 ctgtgttttc aagttgattg ccatgtaaat catcatattg ttcattttcc tttgattcaa
     8521 caaagccttt agcattttcc aattgaatga ctggtaatgt cgtttcaatt tcaacgccgt
     8581 aagacatgac tgcagttact ttagcatcct ccaaaattgc accttgtcgt ttattcggaa
     8641 aatcaatatc gataggtata taagatgcac ctactttaac tgtcgccaac atcgccgcaa
     8701 tcatttcaaa actacgttct gtaaacaagg caacccgttg accattgccc acaccatttg
     8761 ataggagcat gtgcgcaatg gcatccacat agttgcgtaa tgtttcatac gtcattgtca
     8821 aatcattcat gactagcgca acatgattac cttgtcgtga gacaacttca ttaaagtaac
     8881 ttatgataga tttatttccc gggacattaa gcattcgatc gttaacatgc gtattgaccc
     8941 aatttagaag ttcctccgtg ccgtttggta tatcacaaat ttgtagtgta tcttgatgct
     9001 tcaaaatata atcaatcata atcatacatt gattacccat gtgacgaact gtttctgagt
     9061 gatataaatc ggtattatac tcgatattga ttgtatagtc atcgcgatct tcttcaatga
     9121 tgaaagataa atcaaatttc gccgtcactg atttgggttg aatgtgtgtt aatttactat
     9181 gcccaaaatg agcatgattc gtttcattgt tttgtagtac taacatgaca tcaaataatg
     9241 gattccgtga ggcatcatgt gattgatcta agtcatttac taaacattcg aatgggtatt
     9301 cttgatgctc gtatgcctcc aaactcattt ccttaacctc ttgtaaaaac tgtgtccaca
     9361 ttttatcagg tgacggttgc cctctatata ccaacgtatt agcaaacatg cctagcattt
     9421 gctccgtgcc tttatgcata cgcgcactca tcacactacc gacaacaaca tcatcttttc
     9481 gagcatatct acttaacaac gtcatgacca cactcataaa gaacataaaa tcagtaattt
     9541 gatgcttttc tacatacttt tgaagtagct gtctcatttg ttgattcatt gtaaatgaca
     9601 tcattgctcc atttgtcgtt ttaatatttg gtctaacata gtctgtcggt aagcttaaaa
     9661 taggtacttc atctttgaat tgagataacc aatattgtct atgtttcgtc atatcacgat
     9721 gcgacatcca ctcactatag tctttatatt gcaatttaag tggtaacaat aatttatgtt
     9781 gataaagtgc gttaagatca ttcattaatt gtatattact cataccgtca ttaatgatat
     9841 gatgcgtatc tataaagagg tatgcatgta agggacttct aatgtatctc actctaattt
     9901 gacttggctt ttccaaatta aaaggtgcta caaattggcg catgatttct tgttcatccg
     9961 taaaatgcgt gttaacttct tcaaagtcaa ctgcaacatc tgccacaata cgttgtcgaa
    10021 cctcatcatc tacaacaata tattgtgttc gtaaaatctc atgtcgcgct atcaaacgct
    10081 gcactgcttg tcgcaattga gctacattaa gttctgatga taaccgccat aaaaaaggta
    10141 cgttatacac cgtatcttta tggtttgatt tccataataa atacatacgc ttttgtgcag
    10201 agctcagcac ataatcatct ttaactatag tttctggaat cacttcatag ttttgttctt
    10261 gaaccttagc aatcgcttgt gctagttcaa atacagttgg cttttgtaat aaatcaccaa
    10321 tttgtaatcg tttcccagta gatgcctcta tccgattcac cactaacgtt gcttttaatg
    10381 aatggccacc taattcaaag aaattatcat gaatacctac ttgattcaca tgtaaaatat
    10441 ctgcaaaaat ttggcatagc aagtgttcgg tatctgtact cggtgctaca taggcatccg
    10501 tatcgacata gtccatgata ggcaatgcct tcttatctaa tttcccatta atagtaatag
    10561 gaatttgctc aatatgcatg aaattaactg gtatcatgta ctccggtaag gtcatacgta
    10621 attgtgattt aatcttatta tgtgataatg tatgcatcgc ttcataataa gcaacgatat
    10681 actgatcttg atcatgattt tgaacaataa caactgcttt attaatacct tgtatacgct
    10741 cgagcgcatg ctcaacctct gacaactcaa tcctaaaccc tcgaatctta acttgtttgt
    10801 cctttcgata taaataatct atgttgccat cgggtaacaa acgaacgata tcaccacttc
    10861 tatacatcag ctgatttata tttgaatctt tgataaattt atctgctgtc aattctggct
    10921 gatttaaata acctgcagct aacccaaagc cacttgtaca taattctcca ggaataccaa
    10981 cgccacaccg acgctcgcct tgcatgatat aaacatgagt acccagaatc ggtttaccaa
    11041 taggaatacg atttggaact ttgttaggta tattatacgt cgttgtaaat gttgtatttt
    11101 cagttggtcc ataaccatta ataatttgag gatgcttcgg tttttgatta agcaaatcca
    11161 cccacttagc attcaatact tctccaccaa ttaataaata ctttaacggt accaatactt
    11221 ctattcgttc actagcaatc tgattaaata atgaggaggt taaccacata gtattaacgt
    11281 cattttcatt gattaattgt tctaccgcta ttggatttaa taattgttct tttttagcaa
    11341 caatcagctt tccaccattg agcaatgcac catatatttc aaatgttgca gcatcaaagg
    11401 ctatagttcc tgataacaaa atcgtcgtct cttcatttaa tggtacataa tgattttgat
    11461 ggaccaatcg aacaatacct cggtgcggaa ttagtgtccc tttagggtta ccagttgtcc
    11521 ccgacgtgta aataacataa gcatgatctt ctaacgtgtt acatttagaa agattatcaa
    11581 tatttttcca cgctatctta ttcaaatcaa tgtgattaat attttgttta ccattttcat
    11641 ataaagcttg gtacgttatt acaactttag gcgttacatc ttttaaaatg tactcctgac
    11701 gatcacttgg atagttcgga tcaattggca cgtaagcccc accagctttc aacacaccta
    11761 tcatcgctat tatcatctca atactttttt cagctatgac agcgacacga tcattaggtt
    11821 caacaccata ctggtttctc aaacggtgtg ctaaatcatt cgcgcgtgca ttcaatgttt
    11881 gatatgttat aaacactccg tcaaattgca cagcgacatg attcggcgtt gcttcaactt
    11941 gttgctcaaa taaggtaaca actgtttgcg catcatctat ctcaggcaaa cttaaattga
    12001 tatcgtcata taattgaata tcacgttctg tcatcaaatt aagttcatct acagttgttc
    12061 gtttatttcc attttcttca gtaatttgca aataaatatt tcgaactaag tcactcagcg
    12121 tctcgattga gagcaaatca tatgctgcac tgttatatat aattttgaac ccgtgttgat
    12181 gtggaaaaat ttcaatatcc gctaaagatg tatgtgcatc atgtatttga tgtacatcct
    12241 caataacatc attacaacaa gacatcatat gatgataaca atgaaaaata gtctctagtg
    12301 aaagcgaaga cttcgcgcac tgtaattgcg acatattttg caacacacat ttattaaaat
    12361 ctgttgtaaa acgttgacat acatcttttg catcgattgt taacgttaac ggcacaatat
    12421 ttccgtgtaa atcatttggt aaatgtgatg gtacatgtat acctaatgtg acatcatgtt
    12481 gttgactcat tatatgatta gctaaataca cactaacagc caacgatgcc atatctattg
    12541 atgtcatatc atcaatcaaa tacgtttgat ataaagcttg ttcaaatgga tgcttaattg
    12601 gaaaataact atcaatatgg atgtcagagt tattctctaa ccgaaaatag tttgagtcta
    12661 atgctatatg cgatgcatct tgattgtctt tatcatcatt tctatttata tgtgcatgct
    12721 gtcgagtatt gttaataaca gtattgccac gatatgcatt gcataaatca tcaagaaaaa
    12781 tatcaatttg actatcatcg aaaatggaca catgaaaatc taatagtata tatgcagcat
    12841 cagcgaactg aaacaattta actttgaata aaggtgaatc attaaaatgg taagtactta
    12901 attcttgctt aaaaaaagct tctaaatcat agtttgcgga agaagatgga acttgtttta
    12961 tctcaataaa aggcagaaat tcatgaagta tcattgttaa attgtcatcg gtagtaacat
    13021 caaaaaaatg tcttatagat gcatgttgtt gcacaattgt cgataatgca tacatcattt
    13081 tagtagcttc aacattttta gcgagtttaa cccaatacgc attacggtgt gtcgttgatt
    13141 ctgtattatt tttgtatata cgaaaatatt cctgttgaaa tctcaaatta cccataatca
    13201 taaaaagtcc ttctttcata tcataatact cattacttac tgaaattgca tgatgatatg
    13261 ataaccgacg aaatgttaat taactcgtta tgtaatgatt aatataaaac accattcgca
    13321 acatatgagc gatatattct accctaaaat acatcttgta tcatcgttac aattggtata
    13381 tttttcaatg taaattacat acatcttcga taaatagcac actacaactc gttaatcact
    13441 ttctgttgtt cacatctcat tgcaaactca atattgttgt tacaaaatat ccatgaagca
    13501 agtttatatt aaacaaacaa ctcgcataaa acaattgtta tccttaaatt ttaacaaatt
    13561 cttaataaat ttatctctat tttaattacg accaaattaa taggttttcc atataaaaag
    13621 atgcataaaa taaatattta aataaattca atttgtattc acttgttttt gtcccccaaa
    13681 tacaccagca acaagcatgc tagcaccaat tgttaaaacg ataaacatat acagtcccat
    13741 ttgtaatgac gttaagaaaa cacccaacac aatccctaac ctagctagtg tttctgaaaa
    13801 atgaatacct aatgcattaa ctgcactata tgttcctctt ttagctttag gaataatttt
    13861 aaagcgttgt tctgaaacta taggcgaata aataatttca cctacagtcg caattatcat
    13921 aaaaacaact aataagccaa actgattaag ataggttaga ccactatagc caacaatata
    13981 aatcagcaaa ccagtgatta aagctttttt aaaatctatt ttcaatacaa ctttcgagat
    14041 tgaatacgtg agtaaaatga cgacgaccgt attaatcatt agcaagattg ctaacatctt
    14101 agcacctgta atatcatatg aacctatact tattgtttca aactgatcct ttagtctaat
    14161 agcaatatat gaggagattg aaaattcacc catcatgatg atactgaacc ccgaaatcaa
    14221 taacatataa ttacggtctt tcaaaactaa tttataactg cgaaatatat tcattatttg
    14281 taatttttga taacgacttg catgcctctt gtcatcactt tgctttactt gatttcggtc
    14341 ttgaggtaac caaatatata aaataaagag tacaattaaa aatatacaag ctgctattaa
    14401 gaaaagtagt aacatactgt agccatacat caagccacct aacaatgccc caatagctac
    14461 cgataagttt gtcatccaat agctaatctt gtaaatataa tgttccacgt cttcggtaat
    14521 tgcatccata attaatgtgt ccataactgg aaattgtaat ccccaaacga ttgtaaatat
    14581 ggcatatgca acacaaaaac caataatttg ccacaattga tgtgacccaa atacgcccat
    14641 gaacacaagc attatcacca tcgtcgcttg ataaataagt actagcaact ttttcggaaa
    14701 tatctcaata aggtaaccag atataatgga caatggaaat tttagaacca ctaaaccaac
    14761 aagatatata ccgacaattg attgacttaa catatctgtt aaatatagtg ctataaacgg
    14821 tataaatgct gtcgtaataa ttagctgtaa aaaattgcta atcaatcgta ctttcaaagt
    14881 cgtacttaag cgtttcatta ataactacac ccctttccac atatatacac actaccattt
    14941 atataaaatc tattattaga ttatacgttg aaacaaagaa tttttagtta tacaaagtta
    15001 tttaacaaac aaatgcatcg actttacaat gcatcttgca tataatgcct caaaaaaatt
    15061 agcactatat gactatatat tattccaaat gaatgaaatt tatcaataat tgcgtaagtg
    15121 aacacatatg tgcgtaaatg ttaaatcatt gtcctaacat agctgtgact atacttagat
    15181 gtaatcataa aaaaactact gccatcttta caacagcagt agtgcttata tgattcattt
    15241 atataaaatt gaaagcgtta atcgctagcc cagcatcctt attctattta gctgtataac
    15301 ttttactagc aatacgacca cttgcaacaa taatatcttt atcttggaaa ggttcatgat
    15361 tgaagaaacg ctctaaaata tctttaactc catcttcaat acgtttttgt gcttctaaag
    15421 tcatacctga atagtgaacc gtcatagcat ttctaggcat tgttctccat ggatgatcag
    15481 caggtgcagg ttgtggatac caaacatcac cagcatatcc ttgtaaatgc tcggatgcta
    15541 acgcttcaac taacgcatcg cgatttacaa ttttaccacg tgcagtattc actaaataac
    15601 tgtgtttttt catacgactt aaaacatctt tatcaaataa gttatcagtt tctggtgtta
    15661 atggtgcatg aattgtaatc gcatcacttg ttgaaacaag ttcatcaaag cttacaaatt
    15721 tagacaattt atggtcttgt tgattgattg gatcatagtg ttgtaatgtt acattaaatg
    15781 gcgctaatct ttcagcaaca agttgtccaa ttcgaccaaa tccaaaaata ccaattgttt
    15841 tgtgttgtaa ttcatgcgca tgattaccta cttgagacaa gttccattca ccttctactg
    15901 attgacgatg accttcttca tagtttctaa gaagtattaa taaatccata accgcatgtt
    15961 ctgccacact aactgtatta cttcctgtaa cttcaacgac accaatattg tgttcacttg
    16021 ctgccgctaa atctacatgg tcagatccta cacctgctgt aattgctaat ttcaagttcg
    16081 gtgctttttc aatacgttca cgagtcatat atgcaggata aaatggcgca ctaataatca
    16141 catccatatc tggtaaatgt ttatctaagt cttcaccatt atctgctaat ataatgaact
    16201 catgtcctct ttcctctaaa aatgttttta atcctaatgc ttttttagta ttaagtaatt
    16261 gattttcttg accttctact gcttctggaa ataatgctac gattttcata tccatctctc
    16321 ctaaatgata aattttgact tccttaaagt acgtgttgca tgttgcttgt tttaaaaata
    16381 tgacaaaaaa acggcaccgt ttgacatacc acccccttga attattgtta aattcaaagc
    16441 gaagttagca aacagcaccg tgtaccttac accagcaaca cttattctat tgacttatcg
    16501 aaaacaaata ttgaaatgac ttcatcatca ttcaaatcaa tatctgtaaa taattttaca
    16561 aacttagcgc ctacaagact ttccatctca tttacatcta tttctttata catctgttta
    16621 atcttctctg tgcgtgtata atggagcatc gattcattgc gtttgtcgtt taggtaaaaa
    16681 ctctcaactt tactcaaaac acctgtcata tgtgcaatcg cccaattatc tttaaacgac
    16741 actcgaatac tattgggtcc tttacccacg tattctttac gataagatct tactagattc
    16801 gagaattgtt gttctttagt atgcgtatct atagtattca ccttctttaa aataagaagt
    16861 tcattaattg ttcacatttt cattatacac cttattaaca ctttttcaat gataacgatt
    16921 attattatta cgaaatgtat attttcctag ttaaaaggat ataacaaatt aacagaaaat
    16981 gtttataaac taagatttat attattttaa aaaatgtttc aacaaacgca tctaaacaaa
    17041 aaatcagctt atcacactta ccccttcaac tctgcttcaa gtttacctaa atgtcttaat
    17101 gtcggtgtca atgctgcgaa gaagaatcct tcttttaact tgcgaacttg tggcgaatat
    17161 ggtgtgtacg ctctagaacc accattgaca acagaagctt gatttacatc taacaataaa
    17221 tagccgatgt ccttcttcaa tgatattaat tcatttaaat gtgaagttaa gttaccgtca
    17281 tctaatattg cataatattc ctctctaagt tgacgataac gttttttaaa agcttcaaca
    17341 tcatactcta aatattgatt tattccgttt tgcacatttg aaaatgcatc aattaactct
    17401 aaagaacttt taattgagcc taatcctatt ggaatttggt aagcaataaa ttgcgggcga
    17461 atagttgccg caaactgctt cgcatcatgc gtgataattt gtgattgtgg cactacgact
    17521 tgattcaatg tgatttgata cgttgctgac ccgttgactc ctaaaaaatt ggttttttca
    17581 acaagagtga taccatcttg attggcacgt agaatgaaca tgacaaattc atctgatgat
    17641 tcatgtttcg aaatcgcacc aaaataatgg tcttcttgaa tattacttac agctggcata
    17701 cgtccactga caaccaattg tccatcaaca taagtgtgtt caaggttcaa cttttctaaa
    17761 tcattaaatg acttcatcgg attagacaat ccggtagcac ctaatatttc tccagataac
    17821 aattgctgtt gtaagtcatt atttaaatgt ggctgcgtgg cattttctaa atacgttgaa
    17881 aaagctaatt ggcaccataa acaaaatcct gttgtcaaac aagactgcga tacagcttcg
    17941 attacttcag cattttttct caatgccgcc tcaccgaaat aaccatctac aaataattgc
    18001 tgaataaatg ttctcggata atacgtccct tcatcaactt ctactaaatg aggatctaat
    18061 tgttctttga taagcgtttc taaagtcatc atcatctctc cttattcacc aaatcttcta
    18121 agtataaact gctcaatata actaatgaat cgatcaataa taaaaccaaa taatccgata
    18181 aagaatattg ctgctaaaac atcttctaag ttcaacatat ttcgtgcatc aacgattaaa
    18241 aaacctaatc ccgattgtgc accaatcatt tcaccagaaa ctaaaaatat ccaacttgtt
    18301 cctaccgcca tatgtatccc agccatgatt tgtttaaatg ccccgggaaa taatatattg
    18361 cgatacaatg accacccagt taaatttaaa tttgctgcta tttttaaata ttgaggttca
    18421 atgtctctaa cgcctttaat agtattgaac acaattggga aaaaagcagc gataaaaata
    18481 atcgcaatcg ctggcaaact accaatacca aaccatagaa caacaaatgg tgcccatgct
    18541 atcggagata tcggcctaat caattgaaat agcggttcga tagcgttgta tagccaacga
    18601 ttccttccaa gcaagaagcc caatggaata gcaaccaaca atgcgacaac aaagcccgct
    18661 acaaatctcc ataaactaat tgctaaatgt tggaaaattt ctccagtaac aatgaaagac
    18721 catatacttt ttcctacaag agcaggaccc ggtaacaata caggttggta atgcccaata
    18781 ataatgacca tttcccaaat gcctaagaaa ataataaatg tgataatagg taatataaat
    18841 ttgttatttg tgggacgtgt catgaacgcg atgcctcctt atacaatgac ggttcaacaa
    18901 agtcatcata tgcaggtgga ttaaacaaat gatgttgttt taccaatgtc gtaatttctt
    18961 gatagccgga tggcttaatt gttaaatcac cataggatgt ccatgccgct gactgtgtta
    19021 aaacgtcacg actttgttta aaatgatgcg tcataatgtc tacactttgc ttgcgatcat
    19081 tcattttaaa gccagacttt ttataatctt gtacaaatgc ttgcgctaca tccttgtgtt
    19141 gatcaagcaa ttcccctctc agtactagca cacagcaata cgcatcaggt ataacgtcat
    19201 caccatgttt caaagtctta cctttgccta acttttcacc cagtgcaccg aatggttcgg
    19261 ctacagaata ccctgtaatt ctgtgttcac tcaatgcggc tggcatttct gctggcgaca
    19321 tttcatgata gctaaaatgc cccggtttaa tctttaattg tttacgtaat tcctcaagta
    19381 aaagataatg tgttgaataa cgatgtggta taccaaaatg gtaatcatcg ccattattat
    19441 taaattcatt taagtgcata cctttttgtc ccataatgac attgccttca tgatggccca
    19501 atgccacagc ctttatattt gagcccttct gttttgattt catcgctagc tctattaaag
    19561 ttgatgcacc atcaatacga ccactgttta atgcgtccat taaatctggc caattattga
    19621 atttaactaa ttctagttta tatttcggat gattgtattg tgataataat tttttagtca
    19681 tcatcaaatt agctgaatgt gtaatcggca aatatccaat tttaatcact tgctgatttt
    19741 gggcattttt agaccgttct ttagacgtcc tttgccaatc acatcctgta attataaaga
    19801 ttccaatgat gacgattatg cttaaccttt tcatcgtcac tcactcctta taaataatat
    19861 tcaggttcaa cttgatgatg attcaatgca aatgtttcca taatttcatt acgaatctta
    19921 agtaggtggc tatcattacg actgcgtgga tgtgatgctg taatttcata ttgagaaata
    19981 atattgcacc cttcacctaa cagaacaatg cggtcggaaa gataaatagc ttcatcaatg
    20041 tcatgcgtca ctaaaataat agttgattgc gttttatgtt ttagttgcac tagttgatcc
    20101 tgaagtttat aacgtgtaaa tgcatctaat gcacctaatg gctcatccat cgatataacg
    20161 ttaggcttat gcacatgcgc tcgacatagt gccacacgtt gtttcatacc cccggacagt
    20221 tgctcgggaa aatgctttcc cctgtcttct aaatcaacta atttaagctg tgcgttaatc
    20281 tcttcatcac taattttctg ttgtaatcca atcctaatgt tgtcattaat cgttttccat
    20341 ggcagcaaat tatgatgttg aaatagcatt aaacaatctg gagatggctg ttgtttaatt
    20401 tcgttatcaa taatgacacg accagacgat ggatgaataa atccaccgat aatattgagt
    20461 aaagtagact ttccgcaacc acttttccct atgaaagtga ctatttctcc cttgctaatg
    20521 tccaaattaa agttatgaat tactttatgt gatccaaagt gatgttgtaa ttgttgaatt
    20581 ttaatcatga caagccccct tttaacccct actattttaa taggaattaa gtgtatatct
    20641 aaagggctat gccaaacaca gcccttatat cgtgaattgt tatatctata gatgattatg
    20701 gtttggtatg agtgctctta taacataagc gttattcaat taacgacgtt atattaattt
    20761 cagctctact aaatttcaat aaaatgattg ttttagacaa tacttgcatt tagttcatta
    20821 gtttcttcat atcaactact tacatttata gcattgagct attctaatta actgcaatcg
    20881 aaatccatgc caaacctata tacatatatc tcatcaactt gttgtcttat taaagttttt
    20941 cgatgccatc aatcggtgta tcaactgctt tgcgcgcgcg acgtaccgct tcttcatcag
    21001 gtgcgttgta taaacaaata catttagaca tatcttcaca tacatatgtg cgtttaaatt
    21061 caacttctgg cacttcttca taatgaacag aatttttctt tttacgtgct aaatattgat
    21121 ccatcgtaat gccttccgga atgttccaag ttacaaggta atcaacatgt gcatcaccat
    21181 ttttaacttc atctaaatct ttaccaacta agcgtacttc tttgactaat tgaatactaa
    21241 tatctgcttc tgttaaaaat tgtttagctt ctgcttcgtc atttgcttcc acaataaaat
    21301 aaccatgagt taaattttca gtagcttgta cttcaattaa tgttggaatt gtcttcgtag
    21361 atagtgatgc tgcctttgct tctagttctt ctttcgttga tgcaaaatca agattgttag
    21421 cttctaataa aaataatgtc attaataaga cctcctataa caatggttga aaa
//
LOCUS       DAKTGS010000033        20685 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.033, whole genome shotgun sequence.
ACCESSION   DAKTGS010000033 DAKTGS010000000
VERSION     DAKTGS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.033"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            745..1140
                     /locus_tag="O0286_001056"
     CDS             745..1140
                     /locus_tag="O0286_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169440.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            1336..2721
                     /gene="fumC"
                     /locus_tag="O0286_001057"
     CDS             1336..2721
                     /gene="fumC"
                     /locus_tag="O0286_001057"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCY0169441.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(3184..4005)
                     /locus_tag="O0286_001058"
     CDS             complement(3184..4005)
                     /locus_tag="O0286_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0169442.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            4168..5280
                     /locus_tag="O0286_001059"
     CDS             4168..5280
                     /locus_tag="O0286_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCY0169443.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            5302..5925
                     /locus_tag="O0286_001060"
     CDS             5302..5925
                     /locus_tag="O0286_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0169444.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            6281..6745
                     /locus_tag="O0286_001061"
     CDS             6281..6745
                     /locus_tag="O0286_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0169445.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            6924..8048
                     /locus_tag="O0286_001062"
     CDS             6924..8048
                     /locus_tag="O0286_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCY0169446.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            8117..8461
                     /locus_tag="O0286_001063"
     CDS             8117..8461
                     /locus_tag="O0286_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCY0169447.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            8844..8942
                     /locus_tag="O0286_001064"
     CDS             8844..8942
                     /locus_tag="O0286_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169448.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            9233..10429
                     /locus_tag="O0286_001065"
     CDS             9233..10429
                     /locus_tag="O0286_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0169449.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            10419..13355
                     /locus_tag="O0286_001066"
     CDS             10419..13355
                     /locus_tag="O0286_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0169450.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            13352..14293
                     /gene="yhaM"
                     /locus_tag="O0286_001067"
     CDS             13352..14293
                     /gene="yhaM"
                     /locus_tag="O0286_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCY0169451.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(14414..15376)
                     /locus_tag="O0286_001068"
     CDS             complement(14414..15376)
                     /locus_tag="O0286_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCY0169452.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(15581..16138)
                     /locus_tag="O0286_001069"
     CDS             complement(15581..16138)
                     /locus_tag="O0286_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0169453.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            complement(16871..17236)
                     /locus_tag="O0286_001070"
     CDS             complement(16871..17236)
                     /locus_tag="O0286_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0169454.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(17378..17800)
                     /locus_tag="O0286_001071"
     CDS             complement(17378..17800)
                     /locus_tag="O0286_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCY0169455.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            17934..18674
                     /locus_tag="O0286_001072"
     CDS             17934..18674
                     /locus_tag="O0286_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169456.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            18667..19890
                     /locus_tag="O0286_001073"
     CDS             18667..19890
                     /locus_tag="O0286_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169457.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(20014..20517)
                     /gene="traP"
                     /locus_tag="O0286_001074"
     CDS             complement(20014..20517)
                     /gene="traP"
                     /locus_tag="O0286_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCY0169458.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
BASE COUNT         7457 a         2956 c         3489 g         6783 t
ORIGIN      
        1 tcatgtaaat aatgtatagt atagaacata tatcaaatag gattgattat cttgaatatg
       61 aagttaggta gcattttgtt acctagcttt tttattatat tcatagaagc tgactttctg
      121 ctagtttcag tgttggggct gtttccccaa cttacattgt ttgtagaaat tgaggatcca
      181 atttttctat gttgtggccc cgccaacttg catgttattt taagctgact ttccaccaac
      241 ttctgagttt gtggcccgtt tccctaattt gcaatgtctg tagaaattag aaatccgatc
      301 tcactatttt ggggcctcgc caacttgcat gttattttaa gctgactttc caccagtttc
      361 tgagtttgtg gcccgtttcc ccaatttgca atgtctgtag aaattgggaa tccaattcca
      421 ctatgttgga gctccaccaa cttgcatgaa atagaagctg acattccgaa gcctctatgt
      481 tgtggactgc agtcaactac tgccaatata actttgtaaa gcataaaaca ttgatttgtg
      541 taccagacat agtaatatat caaaagttca attttaaata ttaaaacacc atttaaacgg
      601 aattgataat taattactcc agacataatt gaaagaagtc atctatcagt aatattttta
      661 atgaaatcat taaaccgcgt ttcttaatat gccaagatta tgatatgcta ttgaagaatg
      721 aaattagagg agatttatat atttatgtta ggaaaatact ggattcaatt attgatagca
      781 acagttatta taagtttaat atcaattaaa gcttttccat tggcaattgg ggcattatat
      841 ttacctgtta ttttcaaagt aataaaatta caactcaatt tatcaaaagg attaattgat
      901 gatgttaatg cgcaaacatt cattaaaagt aatcaatcag gtattgtaat cagtgtaatt
      961 tgttgtttat taataacagc aatattgtat tatacacttg atgggtttta tgcgagttta
     1021 acaggagttt taggtacgct tgttgctcta aatccatata cgacaattgt tagtgctgta
     1081 ttatatatac ttacagctat tgctacggtt gaagctacta aaacaaagta tcgtaattaa
     1141 acttagttca ataataacta aataatgata gataacacac taatcgtatg accgtatcac
     1201 tgtaataaag ggtgaacacg tgcgattagt gtatttttat ttcaaattat gaaaacgttt
     1261 taatcataaa ttgctcaatt aaattattta atgatatact acagttaaat aaacagtgat
     1321 aaggggagaa attgaatgtc agtaagaatt gaacatgata cttttggaga aatagaagta
     1381 cctgcagata aatattgggg tgctcaaaca gaaagaagta aacgtaattt cccagttggt
     1441 aaagagcgta tgccaatcga agtagtttat ggttttgcac aactaaagcg tgcagcagca
     1501 atagctaatt ttgatttagg aaaattaagc gaggcaaaga aagatgccat tgtatacgca
     1561 tgtgatcaaa ttttatcagg tgaattagat gaacacttcc cactagttgt atggcaaaca
     1621 ggaagcggta cacaaagtaa tatgaatgtg aacgaagtag taagttatgt tgctaatatg
     1681 tatttaaaag atcatcaaag tgatgaaagt atccacccaa atgatgatgt aaataaatct
     1741 caaagttcga atgatacatt cccaactgct atgcacgttg cattatatca agaggttgaa
     1801 acaaaattag aacctgcatt aaaactttta agaaatactt tgaaagaaaa agaagataaa
     1861 tttgattcaa ttattaaaat tggtcgtaca catttacaag atgcaacgcc gatcaaacta
     1921 ggacaagaga ttagtggctg gcgttatatg cttgaccgtt gtgaaacaat gttatctgaa
     1981 tctaagaagc acattttaaa tcttgccatc ggtggtacgg ctgttggtac tggtattaat
     2041 gcgcatcctg aatttggtga taaagtggca cattatattt cagaaaatac gggttatcca
     2101 tttgtatctt ctgaaaataa attccacgca cttacagcgc atgatgaagt tgttcaattg
     2161 catggaacat tgaaggcatt agcaggagac ttaatgaaaa ttgctaatga tgtgagatgg
     2221 ttggcttcag ggccacgagc tggtttggca gaaatttcta tccctgaaaa tgaaccaggt
     2281 tcatcaatta tgcctggtaa agttaatcct acacaatgtg aaatgttaac aatggttgca
     2341 gtccaagtaa tgggtaatga tacagttgtt ggcttcgcaa gttcacaagg taactttgaa
     2401 ttgaatgttt ataaaccagt tattatgcat aatacactac aatcaattta tcttttagct
     2461 gatggtatgg aaacatttaa taacaattgt gcagtgggca ttgaaccaat cgaagagaat
     2521 attgataatt atttaaatca atcattaatg ttagttactg cattaaatcc acatattggt
     2581 tatgaaaaag cagctcaaat tgctaagaaa gcccataaag aaggtttaac tttaaaagaa
     2641 tctgcaattc aaactggata tgttacagaa gaacaatttg aagcatggat taaaccagaa
     2701 gatatggtag atcctcatta attaaatatt aatataaaat aaagtttggg cattacaggt
     2761 agatgtagtg ttcaagcttt attttttgtc tatgtcaaat tgaacttata agtctgattt
     2821 gatatagagc catgaaaagg aaaaactttt catggagtcc gttttattat agaacctatt
     2881 ttttatatga tgaattgttg tacatgtatt ggccaaacaa cctgtgataa aaatcttatg
     2941 atgtcatgta ttgtccatag atacacgtaa cgcagttgaa aaagactaca agtcattatc
     3001 caaggatgtt ataaaatgaa taattactaa aatatcaaaa tataaaatcc taagcatcgt
     3061 aaaaaacaaa aaaaccgaac atctgaaaaa attaataatt ttccatctat ccgatttaag
     3121 ttgatacttg atttagttac aatgcggtat tgcatgttta aatatcatgc aaaactaatc
     3181 aaattagtca tccaaagaaa cggaaatgac ttccttagtc aagggatgca caaactctat
     3241 tttgtaactt tcaagttcta attgtcttaa cgttgaatca ccatataaag gatcaccaat
     3301 aacagggtga ccaatttcag ctaaatgcac acgaatttga tgagtacgtc cagtatccaa
     3361 cttgatttca agttggcaca cagcttcttt tatcattttt gaagttaaaa tgtgtgtaat
     3421 tgcacgctgt ccagtaggag acacacgtcg tttattcgaa tggaatttat ctttaccaat
     3481 tggcatatca atcgtttgtg gttttaaagg taaaagtgcc ttaacatttg ctttgtatat
     3541 ccgcgtaata tcattgtctt ctaacatgcg atcaagaatt tttttcatta aaggattttt
     3601 agcaacaatt aataatccta ctgtttcctg gtccagtcga tgaattggtt cgacatagtc
     3661 actatcaata gtgtaaatca catgattcat taaagtattg ctttctttta aatcattagg
     3721 gtgagtctta acacctttag gtttcattac gattgccata tcatcgtctt cgtatttaat
     3781 ttgtgcataa cgataacttg gaacataatt acttttttca tcgatggttg gaacaaatac
     3841 atcgtcgcca gtatgcactt tatccattaa tcgcgcaggt ttatcattaa tagtaatatc
     3901 tttagacata tttaaatgat gtaaatcttt tttaggtacc ttaagttgtt ggaaaatatc
     3961 tcgtaaactt aagtcattaa agttttctgg tattttaaat ttcataaaat cctcctatag
     4021 tctttaatat catatcacaa atcagttctc cgaaatataa ctattgagta atcaaggcca
     4081 ttttatcatg tttacgtcta agatttaaaa atataataac tcaatgtata atagttaata
     4141 cacgtaaaaa tgaattggag cgatttgatg gaacaaagga cgcgactagc tttattaaaa
     4201 gagattgccg aatttttaaa cgaagaaact gaaatgtata gcatgaccca aggtgcgtta
     4261 aaatatttaa ttgagggcag taatttcacg acaggctgga tatttttcat caatagcgta
     4321 ggtgaacatg aacttgtatc acatgtggct ctaccacaat ctttgacagc agaccactgt
     4381 cactatatta aagatgggtc ttgttggtgt gtgaaagcat ttaatcaacg taggctaatg
     4441 aaagcgtcga atatcgtcaa ttgttctcgt attaacttag cctctaaagc atttcctagc
     4501 caaaatgaca atattacaca tcatgcaacg gtaccactta aatcgggtca agaacaattc
     4561 ggcattttaa atgtagcgtc tccaaatact gaaatatata gcgatgaaga tttagagttg
     4621 ctagagtctg tcgcatttca attagggtca gccatcaaac gtatttattt aacagaccgt
     4681 gaaaaagaag cggcaaaaat taatgaacgt aatcgtttag ctagagattt acatgattca
     4741 gtaaatcaaa tgttattttc tgtaaaacta acagcacatg ctgcgtatgg catgtcaaac
     4801 gaatccatcg ctaaacaagc attcaagacg attgaggaaa cgagtcaaaa tgccgttaat
     4861 gaaatgcgtg cattgatttg gcaacttaag ccagttggac ttgagcaagg tttaattcat
     4921 gctttgacag catatagtaa attaatgcat atacaattaa atgtaaatgt agaaggttta
     4981 atcgatttat ctaatgaaat cgaagaaaac atatacagag cattacaaga gtgtattaat
     5041 aatgttaaga aacatgctga tacgaataaa atggatctta cattaaaaca aatgaacgat
     5101 atactatata ttgacgtgat tgattatggt caaggatttg agatcgataa tgttcagata
     5161 gcttcatcac acggtattaa taacataaaa caaagagtta aattattaag aggtaaagtg
     5221 acctttcatt ctcagccaac aaagggtaca caaatacaat tcacaattcc tataaaatag
     5281 cttgatttaa ggagataacc catgaacaaa gtaatattag tagatgacca ttatattgtg
     5341 cgacaaggat tgcgattttt attatccacg attgaaaaca tagaagtttt acaagacttt
     5401 gcagatggag aaacattttt agaatattta aaagagcatg agcaccctga tattgtgcta
     5461 ttagatttag tgatgcctgg catgaatggt attgaaatta cggaatatat taaggcacat
     5521 tatccggata ttaaagtttt ggtattaaca agttatgttg atgatgaaca tgtaatttca
     5581 gcaatcaata aaggtgctga tggttatgaa atgaaagacg ttgagcctca gcaattaatt
     5641 gaaactatta gacgagttat gaacggtgaa aaaatgatac atcctaaggc acaagatgta
     5701 ttcgaaacag ttagccaaaa accacactac acgaataagt tgtcaaagag agaaattgaa
     5761 gtgttacgtg aaatggttaa aggtaaaaca aataaagaga ttgcagaaac tttatttgta
     5821 tctgaaaaaa caattaaaac acatgtcagt catatattta gtaaattaca agttagcgat
     5881 cgtacacaag cagcaattta tgcaatggaa aataagttga tttagaatga gcttttaaat
     5941 atttgtcgaa aaatagacaa tttttaatct gattttgatg aattttttat agcgataggg
     6001 tttattatac agttttcttt gtaaatttga tatcattata aaagtgtaag ttttatcaaa
     6061 tctagtgatt atgcataaaa atttacttca tttactttcg cttcattata aagtaaactt
     6121 aactcttgtt gacttgatca aaatttaata caaatgtgaa attgactctt ttcattttat
     6181 taaaaatata ttaagatgta atctgtgttt caagaaatgt gtattattgc aatttcttgt
     6241 gtccggataa aattccccaa acagaaaggt agataacaga atggatagac agagttttac
     6301 agatttaatt caaacaaaat ttaaaatggt tcgtatagag gctggttata cgcaagatac
     6361 tatggcgcaa acaattggac tttctaaaaa gactttagta caaatcgaaa aagagagagt
     6421 attaccaaac tggacaactt gtatttcaat ttgtgcatta ttcagagact ctgaagtatt
     6481 aaacagtaca tttggctgtg atccattaga aatcgttcaa acaatttcaa gaaatcactg
     6541 tgcatatcca aaccatgcac caacaagtga tatttattgg aataacattg agactcgtaa
     6601 tgggtacatt ttacaaagta acaaagtaag caatatttat cgtgtattaa atccagataa
     6661 ccaaccaatt ttcggtactt ctaaaatgag agaagctgaa acatacttta acagaaatgc
     6721 taaagaagaa ttagtacata tctaggccta tatactttta ataaataata aaagcgatgt
     6781 aacacacctg tttcgagagg tgaggcatcg ctttttgtat gtaaactaat aataaattga
     6841 gcagaaagtg aaatgattaa tgaattaaaa aataaaaata gtggtataat gaataactat
     6901 gttgtcacaa aggagtcaaa attatgaatg cactatttat tatcatattt atgattgtag
     6961 taggggcgat aattggtggc attactaatg taattgcaat tagaatgctg tttcacccct
     7021 ttaaaccata ctatatattt aaatttagag tcccatttac accaggttta ataccgaaaa
     7081 gacgcgaaga aattgcaact aaaattggcc aagtgattga agagcatttg cttacagaaa
     7141 ctttaattaa tgaaaaattg aaaagcgagc aatcacagca agcaatagaa tctatgattc
     7201 aacagcagtt acaaaagttg acgaaagatc aattgtcaat aaaacaaatt acttctcaaa
     7261 tcgatattga tttagaacaa gtattacaaa ctaatggaaa tcaatatatt gaatcacaat
     7321 tgaataatta ttatacaaag catcaaaacc aaacaatagc atctttattg ccaaatcaac
     7381 ttgtaacatt tttaaatcag catgtagata acgcaacaga cttattatgt gatcgtgcaa
     7441 ggaattattt atcatctgca aaaggcacac aagatattaa tgatatgttg gatacttttt
     7501 tcaatgagaa aggtaagtta attggtatgt tgcaaatgtt tatgacaaaa gagagtattg
     7561 cagatcgcat tcaacaagaa cttatacgtt taacatctca tcctaaagca agaacaattg
     7621 tgacatcgtt aattaccaat gaatatcaaa cttttaaaga taaaccattg aatgaactgt
     7681 tagatgcatc gcagtttaat gaaatcgctg aaaatctatc agtgtatgta acaacatatg
     7741 caagcaaaca agcgaataag ccagtggtta cgttaatgcc tcaattcgtt gattatttag
     7801 aaggtcaact ctctagcaaa ttagcaaatt taatcattga aaaattatcg attcacttat
     7861 ctacaattat gaaaaaagtg gatttacgag gattaattga agaacaaatt aatacgtttg
     7921 acttagatta tattgaaaaa ttaattatag aaattgctaa caaagaacta aagttgatta
     7981 tgtcattagg tttcatttta ggtggtatta ttggattttt ccaaggtttg gttgcaatct
     8041 ttgtctaaaa aagcgagatg tggtaaagta atatcgaacg ttttaataaa catcacaaca
     8101 aaggagtgct ataaccatgg cagtaaattt atatgattat gcaaatcaat tagaacaagc
     8161 tttaagagaa agcgaagaat acaaagcaat caaagaagca ttcgctaatg taaaagctaa
     8221 cgaagaatct aaaaagttat tcgacgagtt ccgtgaaact caaattaact tccaacaaaa
     8281 acaaatgcaa ggtgaagaaa ttgctgaaga agatttacaa aaagcgcaag aacaagcgca
     8341 agcaattgaa aaagatgaaa acatctctgc attaatgaat gctgaacaaa aaatgagtca
     8401 agtattccaa gaaatcaacc aaattatcgt taaaccatta gacgaaattt acgctgacta
     8461 atattaaaat aatatttttc aatcctttaa atagaggaat agactgccga cagagtccga
     8521 gactttgtgg gtagtttttt tagttttgat aacggaagtt agaggctctc tgtcaaattg
     8581 ggcagatgca ttcagtaacg gaagttaggc tagtaaacgt gtcctaactt cctttttgca
     8641 ttttgaagtg taaagttttc aacataatac tattagttcg gtcatgtatc ggactgatgg
     8701 aaaagcgttt cacttttaat gactcattaa gaacggcctg aaaatgtttg gcgtattaag
     8761 tgcaatgata gttttgacat ttagtttcta attggtcatt actgccgagc aaatctagta
     8821 gagtaatcat gtaaatcttt aatgtgccat ttgattcact agcggtgtta ataactacgg
     8881 aaattgcatt tccgactgaa atttttgaaa aatatcaacg tacgctacaa ataaaatttt
     8941 aaactgttat aaatgtgtct caatttcata tgttcatcga cgatatgaag cgtattatgg
     9001 taaaatgaag aaataataaa cttgttaata aataaaacat cacgatttga ctaaagcact
     9061 ttattattgt gtagataata gttttttaac gaaataaaaa tggcgactgg ttttaataaa
     9121 tcagctaatg aatcactaca cctataagta tgaatatagt gattagaatg ctttgtatag
     9181 ttggattttg caaaattgat gttagaacct ttaattaagg agatatggat gtatggttaa
     9241 atttattcat tgttcggatt tacatttaga tagtcctttc aaatctaaga gtcatataag
     9301 ccctaaaatt tttgaggatg ttcaaaaaag tgcttatgaa agttttaaaa atattgtaga
     9361 tattgcatta caacaagatg ttgattttgt aattatcgcg ggtgatttat ttgatagtga
     9421 aaacagaaca ttacgtgctg aaattttctt aaaacagcaa tttgaacgtt tacaaaatga
     9481 acaaatcttt gtttatgttt gtcatggtaa tcacgatcct ttatcatcaa agatttcatc
     9541 aaactggcca gataatgttt ctgtattttc aaataaagtt gagacgtatg aagcaattac
     9601 taaatctggt gaaacaattt atattcacgg atttagttat gaaaatagag caagttatga
     9661 gaacaagatt gatgaatatc catcaagtca aggccaaaaa ggcatacata ttggtgtctt
     9721 gcatggtacg tatagtaaat cttcagttaa cgaaagatat accgagttca ttttagaaga
     9781 tttaaacagt aaattgtatc attattgggc tttaggtcat atacatgaac gtcaacaatt
     9841 aagtgatatg cctgtaatta actattcagg taatattcaa ggtagacatt ttaatgagca
     9901 aggtgaaaaa ggttgcttat taatcgaggg tgaccactta aaattaaaga ctaaatttta
     9961 tcctacacag tatattagat ttgaagaagc aactattgaa acggataaga catctaagca
    10021 aggtttatac gaggtcattc aaaactttaa agaacaagtg agagaagaag gaaaagcctt
    10081 ttatcgttta acgcttgtta ttaatagtga gacattaatt tcacctcaag atttattaca
    10141 agttgaagaa atgattacag attatgaaga aaacgaaaat caatttgtat atattgatga
    10201 gttaaaaata caatatgcac aaaatgatga gtcaccttta gttaatgaat tttcagcgga
    10261 attattagtc gatcaaactg tttttgataa agcgatgtca gatttatatt taaatccaag
    10321 ggcatctaag ttcctagacg attatggaac attcgaccat acagcattag ttaatcgtgc
    10381 tgaagaaata ttaaaagctg aaatgagagg tgaacaaaat gataattaaa tcacttgaaa
    10441 tttatggtta cggtcaattt gttcaacgta aaattgaatt taataaaaac ttcactgaaa
    10501 tttttggtga aaatgaagcg ggtaaatcga cgattcaagc attcatccat tcgatattat
    10561 ttggatttcc aactaaaaag tctaaagagc caagactaga accacgtcta ggtaaccaat
    10621 acggtggtaa attagtactt attcttgatg atggcttaga gattgaagtt gaacgaatta
    10681 aaggcagtgc tcaaggtgat gtgaaagtat atttacctaa tggtgctgtg cgtgatgatg
    10741 cttggttaca aaagaaactt aattatattt ctaaaaagac atatcaaggt atcttttcat
    10801 ttgatgtact agggcttcaa gacattcata gaaatctaaa tgaaaaacaa ttgcaagatt
    10861 atttattaca agcaggggct ttaggatcaa ctgaattcac gtcaatgcgc gaagtgatta
    10921 atcgtaaaaa agatgaatta tataaaaaat caggtaaaaa tccgatcatt aatcaacaaa
    10981 ttgagcaatt aaaacaacta gaaagtcaaa ttcgtgaaga agaagcaaag ctagaaacat
    11041 atcatcgctt agtagatgat cgagataaat catcacgtcg attagagaat ttaaagcata
    11101 atttaaatca attatcaaaa atgcatgaag aaaaacaaaa agaggttgct ttacatgatc
    11161 attcacaaga atggaagtct ctagaacaac agttaaatat tgagccaatc acattcccag
    11221 aaaaaggtgt ggatcgttac gaaaaagcac gagcgcataa gcaatcgtta gaaagagata
    11281 ttggtttaag aaatgagcgt ttagctcaac ttaaagaaga agcgactcaa ttagagccag
    11341 ttaaacaatc tgatattgac gccttcatta gtttgaatca acaagaaaat gaaattaaaa
    11401 ataaagaatt tgaacttact gcaatcgaaa aggatattgc gaataaacaa cgtgataaag
    11461 atgaattgca atcaaatatt ggttggtctg aaacgcatca tgacgtagat agttcagagg
    11521 caatgaaaag ttatgtcagt gagcaaatca agaataaaca agaacaagct gcatacatta
    11581 aacaattaga acgtagttta gaagaaaata aaatcgaaga taatgcggtt catagcgaac
    11641 tagattctgt tgaagaaaaa atagttcctg aagagacttt tgaaaagaaa aaagaatact
    11701 cacaacaagt cattgaatta aacgaaaaag agaacttgta tagcaaattg aaagaacgtt
    11761 tcgaaattga gcaacaagaa aaacaaaaac gtcaaaaatt gttacgaaca acgtttattc
    11821 ttttaacatt agtaggtatt ggtttaactg ccttctcatt tatttcaaac aacatgttgt
    11881 ttggtattat tttcgcagta ttaactttag tatttgttat cggtatcatc atgtctaaat
    11941 caaaagaagt agattatagc gaggccatca cagatgaaat cgaagaaatt aaagcacaac
    12001 tagccatttt agatgaaaat tatgacttag attttgatct tgatgagcaa tatcgcattc
    12061 gtgatcactg gcaacaagct ttgaaaaata aagatatttt agaagagaaa cgtcagtata
    12121 ttgaaggtcg tttaaatgat gcgaaagggc gtcatgatga attacaatcg acagttgaga
    12181 acgttaagga tgaattatac ttatcatcta aaatttcaaa tgatttaatt gttgatagta
    12241 tttctacaat ggcgaatatt aaagcattag atcaacatat cagtgattta aatcaacaac
    12301 gccagcaatt ggttcaagaa ttagatacat tctacaatca tgctgaagct gttacaaaat
    12361 cacaatttgt ttatttcaac aaattatctt tattccacga tgtgcaacaa tggttgaaat
    12421 cagctgaaga cacgaatgaa aaatggcgta ttaatgctga aaataccaaa cttgttacaa
    12481 atgaattaaa tcatttaaat gctcaattag aagaaaataa taaagaaatt acagcactat
    12541 tcgattttat caatgttggt actgaagaag atttttacca acatcatgaa gattaccaaa
    12601 cttatactag taatttgagt cgttttaatg atttaactaa atatcttgag aaccaaaact
    12661 attcttatga attaagttct agtttaagtg aaaaaacaac tgcacaactt gaagaagaag
    12721 atcatttatt ggctactcaa gttgacgaat ataatgagca atatcttgaa atgcaagcac
    12781 aagtcagtga tttaagtgca caaatcaatc acatggaaac tgatacaacg cttgctaatt
    12841 taagacatga atatcacagt cttaaaaatc aacttaatga tatcgcaaaa gattgggcaa
    12901 gtttaagtta tttacaaagt ttagttgatg aacacattaa acaaattaaa gataaacgtt
    12961 tgcctcaagt tattaatgaa gcggtagaaa tattgaagca tttaacagat ggcagatata
    13021 cgatgattaa ctataatgaa gattcaatta cggttaaaca tgttaatggt caattatatg
    13081 atcctgttga actaagtcaa tctacaaaag aattacttta tgtagcttta cgtatcagtt
    13141 taattaaggt actaagacca tattatccgt tcccattaat tgttgatgat gcatttgttc
    13201 attttgataa aaaacgtact gaaaaaatgt tgaattattt acgatcatta tcagaacact
    13261 atcaagtact atactttaca tgtgtaaaag ataatattgt tccatcaaaa gaagtgatta
    13321 cattaaacaa aatagaggaa ggcgggaaac gatgagaaat atagagaatc taaatcccgg
    13381 agattcagtt gatcactttt tcttagtgca taaagctaca cagggtgtaa cagcacaagg
    13441 taaagattat atgacattac atttgcaaga taaaagtggt gaaattgaag cgaaattttg
    13501 gacggctaca aaaaatgata tggcaacaat caagcctgaa gaaattgtac atgttaaagg
    13561 tgacatcata aactatcgcg gaaataaaca gatgaaagtc aaccaaatta gactagcgac
    13621 aactgaagat caattaaaaa cagaacaatt tgtagatggt gcacctttat caccggcaga
    13681 aatacaagaa gagatttctc attatttgct agatattgaa aatgctaatt tacaacgtat
    13741 cacacgtcat ttattgaaaa aatatcaaga acgattttac acatatccag ctgctagttc
    13801 tcatcatcat aactttgcga gtggcttaag ctatcatgta ttaacgatgt tacgtattgc
    13861 aaaatcaatt tgtgacattt atccattgtt aaacaaaagt ttgttatata gtggtattat
    13921 tttgcatgat attggtaaag ttagagaatt gagtggtcct gttgcgacgt cgtatacagt
    13981 cgaaggtaac ttattaggac acatctcgat tgcgagtgat gaagtagttg aagcagctcg
    14041 tgaattgaac attgaaggag aagaaatcat gttgttaaga catatgattt tatctcatca
    14101 tggtaagtta gagtatggtt ctccaaaact gccatactta aaagaagcag aaattttatg
    14161 ctatatcgat aatatcgatg ctagaatgaa tatgtttgaa aaggcatata aaaaaactga
    14221 caagggtcag tttacagata aaatatttgg tcttgaaaat cgtagattct acaatcctga
    14281 atcactcgat taagaattaa aagatatcgg acagatgagt atactgaaac atgagctcat
    14341 ctgtccgatt tttaatgcga aaaaaccgag cggccgtggt atgatttttg aaccacggtc
    14401 gctcggtttt gtgttattgg ctcatgccgg attgtccgcc ttgtgcgcca ccttgtttaa
    14461 gtttttcagg gtttaagatt ttatcttcga caactgattt aatatcacga tctttaaagt
    14521 caacatcgta ttcttttaat agatctttgt atgcatcagt caataatttt ggattttttt
    14581 gtactttctg atcgactaat ttttctttca ggctttgttt ttcactgtta aagtctgttg
    14641 gtttatcagc tttaataata tgatatccaa agcttgattt aacaacctct gatacttcac
    14701 catctttaag cttaaatagt gctttttcaa aatctttatc agtttgtcct ttaagaacat
    14761 aacctaattc gccatctttt ttagctgaac cagtatccat tgattctttt ttagcgattt
    14821 caccaaattt acttggatct tttgaaactt ctttttgaat ttcttcagct ttttgtttcg
    14881 cttctttatc atctaagcct tctttgtcgc ttttcttaga tttaacttta attaaaatgt
    14941 gtgaagcttt cttgctgtct tctttaattt cagaatcaga gattttaatt ttatctgata
    15001 gtaattcttt atgataagca gcagtacgta aattttcttt atatttatcg gctgttaaac
    15061 cttgctgttg aagggccttt tcaaatttat ctttaccgcc gtattgcttt tgcatttttt
    15121 caatttgttc gtcaatcttc ttatcattaa ctttattttt atatttatca gctaaaattt
    15181 tatttaacat ttcagtaaat gatgcatttg caatttgatc tttaccgatt tttttcattg
    15241 tatctgcaac tgttacgtct ccagctttag aagaaattaa tgtattttct ttagagtctg
    15301 tggcactagc gccacaagcg cctaataata aagcactagc tgttaccgga acgattaatt
    15361 tgtttatcat cttcatagtt gaaactcctt tgtaagttaa tcatcgtgaa tataacatag
    15421 ttttatatta tcaccaatca ataacccatg acatcagact agagcattag gtttaggggt
    15481 agaaattgat gaaaataaaa aaacaaggtc tatatacatt tgattgtaag ctattacata
    15541 gcctaaaaac aaataataac accttgctat aattattttg ttataaatga tatggatttt
    15601 taattttttt caaaatatta atacctgtac tatgttcttc tatatattgt ccataattag
    15661 aaaatataat tatttttaaa tataataaat ctgtaatgga aataccgaaa ttcaatgcca
    15721 ataagaacat aaaataatgt ccatattgcg ggaatgaaat tgttagataa acgaacattg
    15781 cagataagat aattaacggc aatattaagt taatgcaaaa ataaaatttg tgtacaggtt
    15841 gattgacgta ggtattatag aatataagcc attttctttt agttaactta tgaactttaa
    15901 acgattttct atatggtaag aaaaataata aatgtatact tttatgtaat ggatacgtca
    15961 taaatacaag taataaaaag atgagaaaat gtctatctga taatggcgta ttagataaaa
    16021 aatacattac ttcataacta actaaaaaca taatgatggt tgcaactgca ctcataaatg
    16081 caattctagg caaaccaaat cgtgcattga tatcaatttg tcttttacat aaaaacatat
    16141 cttacgcctc atctcttttc aatataagtt agattataac taactcaggt tcagtaattc
    16201 aatagtaaca tatcatgtta gaagaattgt tatataagaa gaaaggagcg acgtattgtt
    16261 tgatttgaaa cgcgaaagtg gggattggta aaattgtctg attgactagc gtagaagtag
    16321 aatgcagaac aaatttctgc gaacacatat tcttgctaat aaagcatcac ttatgaaaaa
    16381 ggcgcgtgtg acatgagtat ttgaaaactt gaataatata aaaaatggat atggcagggc
    16441 atatgcaaaa gcatcttttg accaactaag ttatacaaat gctaaaaata tagtgatatg
    16501 taattataat aaacaccaga ttaataattt gtgcaagtaa ggtatatgta atgtgtatat
    16561 aaggttcggc tcaatgaaaa tataagtatg ttgtttgtcg ataatgattt aagtaaagcg
    16621 taatcaaagt gcaaggataa cacaatgaat cctaagcaat gttttcaatc aattcataaa
    16681 tatgatttaa gcgaaaatca ctacaaacaa tgtcttccta taatttgtaa acataagttc
    16741 atttgagttt taaaaaagag atgacaagaa atatctaatt tctaatgtca tctcgtttat
    16801 ttggcattta attttaaaaa ataaatatgc tgttactaat aaaaatagtt gtgatcgaga
    16861 acgtaacctg ctacttagaa atttcgtttc caatatcctc gccacgattt tgtaaatttt
    16921 cgatgtgtga ctgtaaacgt tcaatattag gattaatgtc agatttgaag tttccaagta
    16981 atgacttaat ttcaccacca attgaactac caaattccgc actttcgttt ttaatttgaa
    17041 caccatagtt taaaatgtca ttaaaacttt gtttaatcgt ttcaaattca cgttgtagtt
    17101 ctgattttga accagtaggg gcagtacgat cgatcgtgtt gttcttgaca cttttgtcgt
    17161 cacgtccttg aagtgcaact acaaaaccag ctgctacgcc aacaccgata ccgaatagaa
    17221 tgcgtgatgc tttcatttat attctcctca cttttaacat ctgttatctt gataactcat
    17281 tttgtttaaa attgctaacc ttgatgttat cacttcaaac gcaattgtta taatattaat
    17341 catattatac ccgttcgagc ttagattaag catacattta aaattgtgct tgaatttgtt
    17401 cagcaatttg ttgttttgca tcgttatcta aaatgtcttc atgtgtttcc cacttataac
    17461 caaatccatc aatatcattt tcgtatcgag gaattaagtg gaaatgaata tgaaatacag
    17521 attgatctgc aaactcacca ttattttgaa taatgtttaa accatcagga ttaaatgcac
    17581 gcttaatagc atttgctact ttaggtaatg ctgcaccgat atgtttcatt gtttcttcat
    17641 cagtttcaaa gatattagca gaagcttttt taggaattaa taacgtatgt cctttagtaa
    17701 cttgtgatat atctaaaaag gcatagacat aatcgtcttc atatacttta aagctaggaa
    17761 tttctccagt taaaattttg ccgaaaattg tttctgacat gatgactaca ctccttttca
    17821 actagtatta ccattcatta taacattgtc gctaaaaatt cgatattagg acatgtgtat
    17881 ttaatatgag cattttataa tttttggtac aatataagtc atggaggtgc cttatgacag
    17941 ttaaagtaga acagcttaca ggtggatatg gaaaacgccc tgtaattaaa gatataaatt
    18001 ttgaattaaa caaaggcgaa attgttggac ttattggcct taatggcgct ggtaagagta
    18061 caacgattaa acatatgcta ggattgctta cacctatgga aggatctcta tcaatttcag
    18121 atatcaacat taatgatgac atagaggctt atagaagaaa gttatcttat attccggaat
    18181 caccggttat ttatgaagaa ctcacattag aggaacacat tgagatgaca gcaatggcat
    18241 atgatattga tcgtgatgaa acgatgaatc gagcaatgcc attattaaag acattccgtt
    18301 tagaaaatga gttgaaagtt tttccgagtc atttttctaa ggggatgaaa caaaaagtca
    18361 tgattatttg tgcgtttatc gttaatcccg aattgtatat tatagacgaa ccattccttg
    18421 gcctagatcc attaggaatt caatccatgt tagatttaat ggttgaaaag aaaaacgaag
    18481 gtagaaccgt tctaatgagt acacatattt tagcaacagc tgaacgttac tgtgatcgtt
    18541 ttatcatact agacgaaggt gaagtcgttg catttggaga tttagaagca ttgagacaac
    18601 aaactggctt acataatcaa acattagacg atatatatat tcatgtgacg caaggtggag
    18661 atgtacatgc gtaaccaagc taccacatta tttaataaaa gattacatgc ccttcgtaaa
    18721 gagaaaaatt attataataa gtttatattt aatggtcatt tcatggtctt tttacttatt
    18781 ttgctgggtg catttatttt tggatatggt gaatggttaa aacatattcc aacgaatatc
    18841 aatttttcat taatagcagc agtgattgtt gcgttgacat ctatttttcc aatgcgacca
    18901 ctgttaaaag aagcggataa aatcttttta ttaccatttg aaaaacacat gtctcaattt
    18961 atgcgtcatg cgattttata tagttacttt gcgcgcatat taatccaact cattattgtt
    19021 attgtaatgt tcccgttgtt ttacaatatt aatcaacata atgtggcatt ttatatttgt
    19081 tttggagtca gtgcattaat ttttccatat gttggtttac gcttaagatg gcaatggtat
    19141 caatcgggac ttaaaacatg gcaagttaat ttaatatcat tcatcacctt tgctctaaca
    19201 tattacttac tattagctcc aaaatggtac atcgcatttg tgatggttgc tttgcctgtg
    19261 ttaatagaat ttttagttaa aaaatataaa ccaggctttt tgtatccttg ggagaaaatg
    19321 attgcaatcg aacatcgaca tcatatgaat tattataaat ttgttaatat gttcaccgat
    19381 gtaaaacatt tgaaagaatc tgcagtcaga agaagttatc tagatatact tttaccagtt
    19441 ccaaagggta gtaaatttaa ctctaatgcg atgtatttat tcttatttat tagaagcttt
    19501 ataagaggga gagacgcatt taatattatc tttaggttag taattattgc agttttatta
    19561 atggtttggt tatcatatcc attagtgaca gcaattattg ggtgtttatt tgtatatatt
    19621 atattgttac aaatggcaca attttattca cagcaagcat atggcttatg gcctcaagtg
    19681 tggcctgtac ctgaagagaa ggtaatcaaa gggtatgaac agtttttata tagattaatg
    19741 tttgttattt gtactgtgtt tgcagtgaca tttattataa aacatatgac gttattctat
    19801 gttgtactca ttttctatat tgtaggttta ttaacgatta gaagtattat taaaaagttg
    19861 aaatatcagg aaacattatt acgagactaa aaaacttcca ttggcatgta tgttgtaaag
    19921 gtgcatgtaa tgttgaacgc caaatgatac ggcgttcaga ttacattagc atctatacgt
    19981 taacagcata accaatggaa gttttcttcg aatctattct tttattgggt atagatatct
    20041 ttcaaaataa cttgaatgtt gtccgcttga accaaagtaa tgacttaatg cgtcttttga
    20101 aaaatggtca ttaaaggcat cagattgctt aaagtcttcg tatgcatgtc gatcagcaaa
    20161 tccgaaataa attttgtatg ttgtaccttt agcaggtctt aacaaacgat agcttttaaa
    20221 gccaccaaag tttctgaaat tatcgtctac actaatcagt ttcttttcaa gttgatatgc
    20281 atgatcttct gttgatggaa tgaagattgc acaatagaaa tgatgttcac tgaattcacc
    20341 aatttcttta ataacttcat atattgaagg tgattttaaa acagtctcac catcagtttc
    20401 ttcaaaaata actgaagtat ctgatgctga aaattggaat agttgatggg tcgggttatt
    20461 gatttttatc tgatgtaaaa atccataagt gccataagat gtatatagtt tcttcatatg
    20521 aaaggtcccc cttatgtttt tatttattat acgatgataa actagtcatt accactatta
    20581 ataattgatt aaaattacta taatgaatct tgcgagatat ttagaagtaa tgatacaaaa
    20641 taatggactt tagaatttaa atttacaata aatagttcta tgatt
//
LOCUS       DAKTGS010000034        20543 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.034, whole genome shotgun sequence.
ACCESSION   DAKTGS010000034 DAKTGS010000000
VERSION     DAKTGS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.034"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..887)
                     /locus_tag="O0286_001075"
     CDS             complement(<1..887)
                     /locus_tag="O0286_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCY0169459.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVI"
     gene            complement(996..1133)
                     /locus_tag="O0286_001076"
     CDS             complement(996..1133)
                     /locus_tag="O0286_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169460.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(1252..2310)
                     /locus_tag="O0286_001077"
     CDS             complement(1252..2310)
                     /locus_tag="O0286_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCY0169461.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            complement(2601..3296)
                     /locus_tag="O0286_001078"
     CDS             complement(2601..3296)
                     /locus_tag="O0286_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCY0169462.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            complement(3582..4262)
                     /locus_tag="O0286_001079"
     CDS             complement(3582..4262)
                     /locus_tag="O0286_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCY0169463.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            4734..5579
                     /locus_tag="O0286_001080"
     CDS             4734..5579
                     /locus_tag="O0286_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0169464.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            5598..5957
                     /locus_tag="O0286_001081"
     CDS             5598..5957
                     /locus_tag="O0286_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCY0169465.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            6481..6633
                     /locus_tag="O0286_001082"
     CDS             6481..6633
                     /locus_tag="O0286_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169466.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            6894..7166
                     /locus_tag="O0286_001083"
     CDS             6894..7166
                     /locus_tag="O0286_001083"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0169467.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
     gene            7565..8269
                     /locus_tag="O0286_001084"
     CDS             7565..8269
                     /locus_tag="O0286_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0169468.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            8662..9198
                     /locus_tag="O0286_001085"
     CDS             8662..9198
                     /locus_tag="O0286_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169469.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(9311..10852)
                     /gene="guaA"
                     /locus_tag="O0286_001086"
     CDS             complement(9311..10852)
                     /gene="guaA"
                     /locus_tag="O0286_001086"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCY0169470.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(10877..12343)
                     /gene="guaB"
                     /locus_tag="O0286_001087"
     CDS             complement(10877..12343)
                     /gene="guaB"
                     /locus_tag="O0286_001087"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCY0169471.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(12381..13649)
                     /locus_tag="O0286_001088"
     CDS             complement(12381..13649)
                     /locus_tag="O0286_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCY0169472.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(13649..14227)
                     /gene="xpt"
                     /locus_tag="O0286_001089"
     CDS             complement(13649..14227)
                     /gene="xpt"
                     /locus_tag="O0286_001089"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCY0169473.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(14458..14560)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            14740..15147
                     /locus_tag="O0286_001090"
     CDS             14740..15147
                     /locus_tag="O0286_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCY0169474.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            15290..15952
                     /locus_tag="O0286_001091"
     CDS             15290..15952
                     /locus_tag="O0286_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169475.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            16070..17026
                     /locus_tag="O0286_001092"
     CDS             16070..17026
                     /locus_tag="O0286_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169476.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(17817..17918)
                     /locus_tag="O0286_001093"
     CDS             complement(17817..17918)
                     /locus_tag="O0286_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169477.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            18003..19391
                     /locus_tag="O0286_001094"
     CDS             18003..19391
                     /locus_tag="O0286_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCY0169478.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(19472..20227)
                     /locus_tag="O0286_001095"
     CDS             complement(19472..20227)
                     /locus_tag="O0286_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCY0169479.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
BASE COUNT         6718 a         3662 c         3169 g         6994 t
ORIGIN      
        1 tctatgacat ctgccatgcg attttcttgt aattttttgt gcaattcaaa cgtgtacttt
       61 ccaccgtttt tcattttaat aacaatttta cctgaaccaa cgttaccgta cagattattt
      121 ttttcaataa gttgttttct caatttaaaa tcaagttctt tcaaggaaat ctgttcttta
      181 gtaatcttga attctgaaac atcatgagag attgtacctt tattatcttc cttagtaatt
      241 cttactcctg ctttgtgatc aactttttta ctattactct ttgtgatacc accgacagaa
      301 tatttttcca gattgtaatt attttcttct aaaacgacaa atacatcgac attcctatgt
      361 actccttcac catatttttt atcatcttta ccaactaaag caattttata tatgaaataa
      421 tctgggacaa cattcataaa tcttattgtc gtccattttt ttaaaataat accaatctca
      481 tttttaaatt ctaaacttgg tttcgtataa tacgctctta aatctttaaa tttaggattt
      541 atttctgttg gtacttgttt tgtggttggc gattgtggtg tgtctgattt agtagattgc
      601 attggttgtg gcgtgtttgt tgatggaggt gttgtcactt tagttgaagg cggtgttgtc
      661 gcatttgctg tttgttgcgg tgcttctact ttagttgagg gcggtgttgt cgcgtttggt
      721 tttgattgcg gtgcttctat tttagttgag ggcggtgttg attgtggtgc ttccacttta
      781 gtggaagatg gtgttgtcgc gtttgctgct tgcgttgtcg ttgtgattac acctgttgtt
      841 aaaaggccta gtgctaaact tgttttagca atcgttgtta ttttcatagt tgtatgctcc
      901 attcgtaatt attagatttg ttcgattaca ttcattgaat catacagctt tattatagat
      961 ggcgtattgc tccattcaca ttaaaccttg tttaactata tttgaatcat cgttaagtaa
     1021 attaagaaat ccataatgtt cgttaaataa aaatgatttt gatgtgattc aacacttggc
     1081 acatttgaag tttcgtcact tttaagacat agaaatgcca cttttacaaa caaatgaata
     1141 ttcgtctttt tacatcatta cgcataataa aagaagctaa gcaacatgta aaccgttgtc
     1201 acttaacttc ttgtttttcc gatgacagct tctatttaga gaatgtcatg attattttat
     1261 attcacttca atgttatcaa tattagtgcc tgccatacga tgctcttgca gttttttgtg
     1321 taattcaaac gtatatttcc caccgttttt cattttaata acgattgttc ctgaacccat
     1381 gttaccgtaa agattatgtt tttcaataag ttgttttctc aatttaaaat caagctcttt
     1441 caaggaaatc tcttccttag taatcatgta ttctgaaaca tcgcgtgaaa tcataccttg
     1501 attatctttt ttagtaatgc ttaattctac tttgtgatta acttttttac tattagtctt
     1561 cgtgatgcca ccgacagaat attttttcaa ttgatattta ttgtcttcta aaacgataaa
     1621 tacatcgata ttatcgtaag gtccatcttt atattttttc tcatcttttc caactaaagc
     1681 tattttatag atgaacctat ttggaataac attcataaac ctaaccgtcg tccatggttt
     1741 gagcataaat ccaaactgct tttcaaattc aaaactcggt tttgtataat acgctcttaa
     1801 atcttcatat ttaggagtca tatctgtttg tgcttgtttt atggttggag attgtggtgt
     1861 gtctgattta gtagattgca ttggttgtgg cgtgtttgtt gatggaggtg ttgtcacttt
     1921 agttttcggc gttgtggatt cggttgtcgt ttgtgattgt tcttgtttag gcgctggcgt
     1981 tgctgatata ttaagcgttt tctgctcttc ttgtttaggt tgtgatattt tttctatttt
     2041 ggaagctgag gttttttcct cattagtatt tggtgccttt tcgagtttag gcgtgcgttc
     2101 ttgtcttgtg ttagctgctt gtgttgtcgc tgaatttgca cctgctgtta tgtttatcat
     2161 tgctaatcgc tctgctttaa gcgttggtac tttgtcaact ttagttgatt gtattttttc
     2221 tgctttgacc gattgcgtcg ttactgtaat tgcgcctgtt gttaaaagcc ctagtgctaa
     2281 actggtttta gcaattgttc tcattttcat aattgtatgc tccaatctat attatattcg
     2341 attgtctttt tacgtaattt gaatcataca acatcattat agatggcgtt ctaagataat
     2401 cacattaaac cccttttaac aattattgaa gtattattaa gtaatttaag caaaaaataa
     2461 tgagtgagta tgagattaat atagcgtttc tatgtgcctt tgaaataatt tttaagcatt
     2521 aaaaagaagt taagcaacgt ttgatcgtca cttaacctct ctatttcaat ttcaacttat
     2581 ttcgtcatca agtatatgtg ttatgctttt ataactttga tttcaattct atcaatatct
     2641 gtgacattga taacatcgga catacggtct tcttgtaact ttttatccaa ttcaaatgta
     2701 tactttccat agtatttctt tttgactgta atttttcctg tactcatttc accgtaaaga
     2761 ccataattat caataaggta ttttcttaat ttaaaatcaa tctctttcaa tgacatcgct
     2821 tctttatcta ttttaaatgg gaaaaagtca taatcatatt caccagtatg atcttcttta
     2881 ataactcttg cttctgctat taggtcgaca gctttatcgt ttgcactcgt gataccccca
     2941 atagagtact ttgcaccttc aaatctctta tcctcattaa cgtaaaatat attaagatta
     3001 cgatgtacac ccgtatgata atgttgctta tctttgccaa ttaaagcaat attattaaca
     3061 gaattaccat ctatgatatt cataaattta atacttggtt gaatgaaact gatataacct
     3121 gtcacatttt tatattcaat actaggttga ttataataag cttttaattt tttgctattt
     3181 tcacttatta caataggttt cttttcggca tgaactggtt tttccgttgt agtgtttaca
     3241 cctgttgcta atattcctaa taacaaactt atttttgcaa tatttttcat tttcatagtt
     3301 gtatgctcca atctattata attagattgt tttattacgt aatttgaatc atacacccat
     3361 attataggag ctgtattcgg atattcacat taacctgttt ttaactattc ataaaatatg
     3421 attaagctat ttaagcaaaa gataatgcgt ggtgcgactg tttgtgtgag ttttgtaact
     3481 tttcccctca aaaaaagaag ctaagcaaca gtacgtcgct taacttccat atatttccat
     3541 aacaagcact attcaacata gcgctttgtt gttagaaagt attatttcat ttctactaga
     3601 attttattaa tcttagtgcc gtcgattgac tcacccatac gttctttttc aagtttttga
     3661 cttaaatcga ttgtatgtgt tgtgccatca ttcattgtaa ttgtaatttg accttgttta
     3721 agaccatttg aatacaagcc gtgttgttta atcgcacgtt ctcttaatct ataatcaagt
     3781 tcttttaatg agatgtcttc ttttgaaatg tagtaaaaat ctttaagtac atcttcatta
     3841 tctttcttga tttctaaaat tggcgtattg atataatcaa tatactgcac actatttgat
     3901 tttgtaacgc caccaattga gtaatttgtt gcttgtctac tgtcaccttc tctaaggata
     3961 aacacatcta tatttgagtt ttctccgtct ttaaatttat ctttatcaga tccaagtaaa
     4021 gtgattcgag aatgctgttg ccctactgtg acttctaaat agtgtttacc ctcatcagta
     4081 tatttaaatc cagtcacatt tttacgctct aaaattggtt tattataata gtgttttaac
     4141 tctgattcac tttgttgttt aacttctgct ttcgcttgta ctgattgtac attcgatgta
     4201 attacacctg ttgctaacat tcccaatgct aaacttgctt ttgctatcgc tttaaatttc
     4261 ataattttta gccccaatct atttaaattt gatttttcaa ttacgtaata tgaacaatct
     4321 acctacatta tattgaggcg ctatcccctt tcaaattaaa cctcatttaa ctaaacttga
     4381 atcattgtta aggtaattaa taaaatgcta actgttatta atcaattctt atccgtcgag
     4441 actctttttt atacaaatca cactttatac catttttatt ttaaaaactg taaaactttc
     4501 agacacaaaa cagacatcat attgtcgtag tacctgatta aaattttcaa tgatattagt
     4561 gatacacatc aactaaaacg cgtcattacg gcattttcaa cattaataat atttacgtat
     4621 ttactataca cgtcgacaat aaatacagat tagaattttt tataaaatca aatgttttga
     4681 aaaccatcaa ataagtatta tgttatgtat ataaagatta ggagatgaag tgaatgaata
     4741 ttatgttaac aggtgctaca ggtcatttag gcacacatat tacaaatcaa gccattgcaa
     4801 atcacataga tcattttcac attggtgtta gaaatgttga gaaagttcca gaagattggc
     4861 gcggaaaagt tcctgttcga caattagatt attttaatca agaaagcatg gtagaagcat
     4921 ttaaaggaat ggataccgtt gtgtttattc caagtattat ccatccatca ttcaaacgta
     4981 ttcctgaagt ggaaaattta gtatatgcgg caaagcagag cggcgttgct catatcattt
     5041 tcataggtta ttacgcagat cagcataata atccattcca tatgagtcct tattttggtt
     5101 atgcagcacg tctattggca acaagtggca ttgactatac gtatgtaaga atggcaatgt
     5161 acatggatcc acttaaacca tatttaccag aattgatgaa tatgcataaa ctgatttatc
     5221 cagctggcga tggtcgtatt aattatatta ctagaaatga tattgctaga ggtgtcattg
     5281 ctattattaa aaatccagat acttggggca aacgctactt attatcaggc tacagttatg
     5341 atatgaaaga acttgctgca attttatctg aggcatcagg cacagaaatt aaatatgagc
     5401 ccgtttcatt agagacattt gcagaaatgt atgatgaacc taaaggcttt ggtgcattat
     5461 tggcatcaat gtacgacgca ggagcaagag gactattaga ccaagaatcc aatgatttcc
     5521 aacaattagt caatgatcaa ccacagacac tgcaatcatt tttacaagaa aatatttaat
     5581 aataaaggag cgttatagtg aatatcatct caacaatttt aatcatattt gtggcattag
     5641 agtttttcta tattatgtac cttgaaacga ttgctacaac ttccaaaaag actagcgaga
     5701 catttaatat aagcgtcgat aaattgaaag acaaaaatat taacctactt ttgaagaacc
     5761 aaggcgtata taacggttta atcggagttt tgctaatata cggtttgttt atcagcagta
     5821 atccaaaaga aatatgcgca gctattttag tgtatatcat tggcgttgct atttatggtg
     5881 gcctttcaag caatattagt atctttttca aacaaggcac attgccagta ttggcactca
     5941 tatcaatgct ttggtaagta ttggtgtttg ggggggtgga gatgtagtcg gaggtttgga
     6001 ggatttgagc gaattgtgtg tggactttag actcagagta tttcatccta attatttcaa
     6061 gcagaggtga cagtagcgtt gcctctgttt ccttatagaa aaattattta atgaagaaaa
     6121 cccatatctg atttaatttt cagctgataa atactccata tattagaatg gctactttat
     6181 ctattgcatc aatcctttaa aacaaaaaac ccatgatttc gaaattcccg tatgatgggg
     6241 ttcctactct catggatcag ttaaataaat attatcacta tcagtttatt atttcaatat
     6301 tattaacaat atatgtagtc gtaaaaggaa agaggacatg agaacttcgg tgttgattgg
     6361 cattacataa cgcttccaaa catattattt ggtaacaata agaaactatt tacacaatat
     6421 attttgtata gtaaaattat tttataatat ttaaatccaa ttgcacaagg agtgattatc
     6481 atggtaccag aagaaaaagg ttctattact ttgtcaaaag aagcagctat catatttgca
     6541 atcgcaaaat tcaaaccatt taagaacaga attaaaaata acccacaaaa aacaaatcca
     6601 tttcttaaat tacatgaaaa caaaaaatct taatcacttt atttatagca tttctaatct
     6661 cagaaatgct ataaaggaca acgccctctt tcataactat ataagtcaag aaaccaagac
     6721 atttcaaaca tctttaaccc cttcaatagc acattcccta aaacataaag caataacaat
     6781 gaacgatcaa aattccttac gtacttatga tgtgtcagtg aatcgtctac acttaattga
     6841 acaaattcta tgagaataga tattgttaaa ttaagaaagt agtcaatttt attatgacaa
     6901 aagaaagaag aacatttagt tcagagttta agttacaaat ggttagatta tataaaaatg
     6961 gtaagcttaa gaatgaaatt atacgaaagt atgatttaaa accctcaatt atctcaaatt
     7021 cgataaaaca acaccaaaat actggaccct tcaatcatca agataattta aaaagtgatg
     7081 aaaaagagtt aataaaatta cgcaaagaag ttcaacattt aaaaatggaa catgatgttt
     7141 taaagcaaat tttaaagttg atttagaaac tgaacttttt aaagatacat tacttgaaga
     7201 ttctaattta tctgattata taaagaacca acttccaacg atatggagag actaagaaat
     7261 agtttaaatt tatttaattt caataaaaac tcataataat atttaagtaa gttagattgc
     7321 ttttagcatt aggaattcgc ttataattag atgttcaata tagacttttt tacacataca
     7381 tgaactataa aaagcgaata aatagcaggt cattgcgaaa tttgctaaat acaaaattga
     7441 tgagttccac ctaataaaaa ttgttggttt tatagtaaca tctattttcg taccattcat
     7501 gttaatttca gtgctaataa tgctgatata aagaagaata aattgattag gagaacacta
     7561 tttaatgtct aaaaatatca atcaagcaaa tagtaaatta aatacgtcaa acaaaaaact
     7621 aaaacaagct tattcaaaat ccgacagtaa aaacatgaaa gttatataca tgcctcattg
     7681 gctagagttt tattcaattg ctatacatag cgatgttaca agcaataaaa aaagatatta
     7741 taaatcatat agtagaggaa cagtggtata tgtaaaacta gggagtaata ttggaagtga
     7801 gttttctggt aatcattttt gtgtaatatt agataacaaa gacaataaag gaaaagaaac
     7861 agttacaata gttccacttt cttccaaagg taataaaaat tatttgaagt taaacgaatc
     7921 ggtgctaaat ctaacagcaa cagatttaaa aaaacagatt atcgatatca gtagtaaagt
     7981 acaagcatta gcccaaaaat atagagaaat tgacattaaa cttagcacag aagataaaaa
     8041 gcttttgtct aacttaaatc aaaaagctaa tgaactgtat agagtgatag gtgtttattc
     8101 caagcacaaa gggaaagata catacgttaa tatatcagca attacaacaa taagcaaaag
     8161 acgtataagc aaaataaatg attctgatcc tactggaacg ataataattt cagaagatga
     8221 catgaatgaa atcgaaaagc aattaaagat aaagttcttt tctaattgaa ttgtaatatt
     8281 acatctgata taattgattt acaaattccc ggtaaccatt ccggcttcat tctcttgaag
     8341 aatgacatat tctcagcgtt ttagctgaag gtcagatgat acgtcatctg gcctcttttt
     8401 ttatataaat gccctttaag tgaaatatac aacaatctgc aatcaatatt agacatcaca
     8461 aaacacacaa taaattacac aatgtgttat aagccacatg tcactattat cgcagctagc
     8521 tgcgtcactc aaaacgaata cggaacgcgt tggttatttc aatcataata ttactctgca
     8581 aatacacagc cctgtaaccg agtaaatgga tagagatttg aacaaatgaa aacaatcaac
     8641 taatggaaag gataaaatat tatgcacaac aataatgaat tcaacaaaaa actgaaagat
     8701 tttataggta gcgataaacg gatggcttta gtaaagggtt atgttaacga gtataaatta
     8761 acaactgttt taagagcttt aaataattcc gattacacaa aagggacgat acacactcgt
     8821 tcaataggca acctaaaaca aatagtagat aatcgaatta ttcctaaaaa tataactcaa
     8881 aacacaatct tcaaattgag taatttaacc ttagaggtta acctctatga aagaaacaca
     8941 atttatcacg gagactttgc tatttactat cctgtagaat ctgctctaat aatagaaaca
     9001 gatactccta ggctaattaa tcatataaat aacaacccag tccaaaaaat atttataata
     9061 acaacaaatg actggagctt taatacactt gaattagaaa aaatagtaga cgaaactata
     9121 atttatgatt taaaacaaga agatcctaaa caatatgaaa ttttatataa aaataaacac
     9181 ggaaaattgc cttattgatg aattaaaacg caaacttaga acaccttacc ccttttccgt
     9241 ccttattgta attttccaac tactcaaatt aaaaaccacc cgtgcaaaca ggtggtttta
     9301 ttatatataa ttattcccac tcaattgtgc ttggtggttt tgatgtaatg tcatagacta
     9361 cgcggttgac gtgatctact tcgtttacga tacgactaga aatcttttgt aagacttccc
     9421 aatcgatgcg tgcgaagtca cttgtcatac cgtcgataga tgttactgca cgaataccta
     9481 ctgtgtgatc atacgtacgg tagtctccca taacacctac tgattgaatg tttggtaaca
     9541 ctgtgaagta ttgccaaatt tctctttcaa gaccttcttc tctaatcact tggcgtaaaa
     9601 tcgcgtctga ttctctaacg atttctagtt tatcttcagt aatttctcca agtacacgaa
     9661 taccaagacc aggtcctggg aatggttgtc tccatactaa atgttctgga atacctaact
     9721 caatacctaa tttacgtact tcatctttaa acaatgtatt gattggttcg attaattcga
     9781 attccatgtc ttctggtaat ccaccaacat tgtggtgtga tttgattgtt tgtgctgttt
     9841 tagtacctga ttcgatgacg tctgtatata gtgttccttg cgcaaggaag tctacacctt
     9901 tcagttttga tgcttcatca tcaaatacgt atacaaattc attaccaatg attttacgtt
     9961 tttgttcagg atctgaaaca ccttttaatt tattcataaa gcgatctttc gcattaacac
    10021 gaataatatt catgttgaaa ccttcaccga attgctccat aaccatgtcg ccttcacctt
    10081 tacgaagtaa gccatggtct acaaagatac atgttagttg atcacctatt gctttatgca
    10141 atagtacagc tacaacagat gaatctacgc cgccactcat cgcacataat acacgacggt
    10201 ctcctacgcg ttgacgaatc ttttcaattt cgatttcgat aaagttttcc attgtccatt
    10261 gacctctaca atcacaaaca cgacggacaa aattatttaa taaatcatta ccatattctg
    10321 tatgacgtac ttctggatgg aattgaacac cataaatgcg acgtttctta tcttcgattg
    10381 ctgcatagtc tgtgcttggg ctatcagcga taacttcaaa gccttctgga atttcaataa
    10441 ctttatcaga atgactcatc caaacagttt gttctgctgg taagccagcg aataactcat
    10501 ctgacttcgc attaatgatt gctttaccgt attcacgttc attggcacgt tcaactttac
    10561 cacctaataa tttagtagtt aattgcatgc cgtaacaaat accaagtact ggaattccta
    10621 aattatatat ttccggatca attgtaaatg aaccttcttc ataaactgaa tttggaccac
    10681 ctgataagat aatacctttt ggattcattt tcttaatttc ttcaattgaa atttcatgat
    10741 cgtgtaattc actataaacg cccatttcac gaattcggcg tgtaattaat tggttgtatt
    10801 ggctaccaaa gtctaagaca aggattaact cttgttcttt tgccatttcc atatttgtcg
    10861 ttctccttta tcttaattag aatgagtagt tcggtgattc tttcgtaatt tgaatattat
    10921 gtggatggct ttctgctaaa ccagcaggac ccatacgtgt aaattgtgct tcttcgcgta
    10981 attctcttaa atcgtgtgaa ccagtataac ccataccagc acgcacaccg cccattaatt
    11041 ggtaaattgt atcttgtaac gcacctttat aagccgtacg tccttcgata ccttcaggaa
    11101 caaatttctt aggcgctttg tcctcttgga agtaacggtc gtttgaacct ttttccatcg
    11161 cacctaaaga gcccatacca cggtatactt tatattgtct accttggaaa atttctgttg
    11221 cgcctgggct ttcttcagta cctgctaata agctacctaa cataaccgca tgtccaccag
    11281 cagctaatgc tttaatgata tctcctgaga atttaatacc accatcagca atgatagctt
    11341 taccatgttt gcgtgcttca gttgcacaat cataaattgc tgtaatttgt ggtacaccaa
    11401 cacctgctac aacacgcgtc gtacaaattg aacctgggcc aataccaact ttaacaatat
    11461 ctgcacccgc ttcaaataaa tcttttgttg cttctgcagt tgctacgtta cctgctacta
    11521 atgtgatttc tgggtaagtc ttcttaatat gtttcacttg atcgataaca cctttagagt
    11581 gaccatgtgc tgtatcgata actaagacat ccacacctgc ttcgactaat ttttgagcac
    11641 gaatatcagt atcttttgaa ataccaattg ctgcggctac aagtagacga ccatgttcat
    11701 cttttgctgc attagggaat tcgataactt tttcaatatc tttaatagta ataagacctt
    11761 ctagacgtcc gtctttaact aatggtaact tttcaatctt atgtttttgg agaatttttt
    11821 ctgcttcttc aagtgttgta ttcactggag ctgtaattaa attttcttgc gtcattacat
    11881 ctacaatttt aatcgagaag tcttcaataa aacgtaagtc acggtttgtt aaaataccta
    11941 ctaagttgcg atcttcttta ttatcaacaa ttggtacacc tgaaatacgg tatttaccca
    12001 ttaatgcttc tgcttcataa acgctttctt ctggcgttaa gaaaaatggg tttgaaatga
    12061 caccattttc tgagcgtttt actttttgaa cttcgtccgc ttgttcttca acgcccatat
    12121 ttttatgaat aacacctaaa ccaccttgac gagccatagc aatcgccatt ttagattcag
    12181 ttacagtatc cataccagca gaaataactg gaatatttaa tttaactttg tctgataatt
    12241 gtacgcttaa atcaacgtct ttcggtaaaa tatcagattg tgctggaatt aataacacat
    12301 catcaaacgt taatgattct tttgcaaatt tactttccca cattaaaaac agcctccatt
    12361 tttcaaatta attagttata ttatttcaca ttttcttccg tttgtttata ctttattcca
    12421 ttaaaaaaga aattaagaat aattgcagag attgcgccta aaacaattcc gttttgagtc
    12481 aaccatgcaa attgttcacc taaaccttta aatgcttgtg gtacagcgct tataccagta
    12541 cctaatccta ctgatacagc gataattaat aaattgtttt gatttttaaa atcgatatgt
    12601 cctaatatac taacaccata tgccattacc atgccaaaca tagctatcat cgcaccgcct
    12661 aacacaggta gcggtatgat atttgctaat gcgccaagct taggtataca accacatata
    12721 agtaataaca cgaccatgcc gtatataaca ttgtttttct tagcgccgga taaagaaaca
    12781 agtcctacat tttgcgaata ggctgtatac ggaaatgaat tgaatataga acctaacact
    12841 atcgctagac cttccgcagt ataaccttta cgaaaatctt ttctttctaa cttcttaccg
    12901 gtaatttcac ttaacgcatg atagacacct gtcgactcaa ttaaactaac gatagctaca
    12961 ataaagaaca ctaacgtcga tgtcacatca aagctaaatc cagagaatct aaacggcact
    13021 gggatgccta accaaccggc atgattgact tgattaatat cgaccatccc aagtaagcca
    13081 gcacctatcg ttcctaaaac gagtccaatt aatatggcaa tactcttaat aaatccagtt
    13141 gtgaatcttt gtaaaagaag aataatgatt aatgtcatta aacctaacaa aatgttctta
    13201 acatctccat agtcctttgc accttgacct ccagctaagt aattcattgc tactggcatt
    13261 aaattgatac caatgatagt aacaacacta cccgttacta ctggtgggaa gaattttaca
    13321 agatgtgaaa agaaaggcgc gatgataata actaatatcc ctgataaaaa tagcgaacca
    13381 taaagtacat ctattccttt cgtttgacca attaaaatca tgggcgcaac agccgtgaat
    13441 gtacatccaa gaacgattgg taatcctgtt cctgttactt tattggcttg taaaaatgtg
    13501 gcaaccccac acataaatat atctactgta actaagtaag cgatttgttc aggtgtaaac
    13561 ttcaaacttg taccaacaat gattggaact aagatagcac ctgcgtacat agctaaaaga
    13621 tgttgaacac ttaggattaa atttttcatt attcttctcc caccaatgtc actttgtttc
    13681 cttctagtga agcaaccttg cagagagaag aaactgttaa acctgcttct tctaaacgtt
    13741 gatgcccatt ttggaaactc ttttcaacaa caataccaat accagctgtc ttagcattcg
    13801 cttgctgtgc gatatcgtat aatcctaatg aagcatcacc atttgctaaa aagtcatcga
    13861 tgataagtac agtatcttct tctgataaaa actcttttga aacaatgacc gtacttgttt
    13921 tatttttagt aaatgaatga atagatgttt cataataacc atccgtcaaa gtgctaggtt
    13981 ttgctttttt cgcaaataaa catggcacat caaaatgcag tgcagccatg attgcaggtg
    14041 cgataccgga agcttcaatg gttaagattt tagtaatccc tttatcttta aattgctcgt
    14101 aaaaagtgcg accaacttca ttcattaact ttgcatcaat ttgatgattt aaaaatccat
    14161 cgacttttaa aatcttctca tcaatgacaa cgccgtcttc ctttactttt tgtcctagta
    14221 actccactct taaaacctcc tcagatttgt gtgaaacatt ttgctcttaa attggtgcta
    14281 gatacaaaaa aatccccaaa ctaaataata gtttcagggt ttatgagtga acgaacatgc
    14341 ataacgaatt tgtcatgcaa tcaatgtaaa agaagtttca tcaaataaac tgtgaccatc
    14401 atataaaatg atataaatca cccaccatgg ttacaattta atggctgaag ctactcctag
    14461 tattgtgttg ttactcatag tcatgtcgtt caaggcaaca tggtagaaac ttctaaagcc
    14521 atattcttta gattatatga gtttatgtaa attatttaac gataatagca aattttcggc
    14581 attttttcaa taactgctta ggtaatcttt taatagtttt ataaatgtac agagacgttt
    14641 aatgcgaatg aagacttata acttaataat gtaagggttt tactttataa taagaagggt
    14701 aaaagattaa ataaacaaat ttttaaaggg gcgacaacta tggcagatat tactgtagta
    14761 aatgacactg gcgaattata caacgtaatc aatcaaaaga aatctgaagg ttatttagaa
    14821 tcagagctaa caattattag taaaagtaaa ttacacctaa atgacttaca cgactctgaa
    14881 atctccctta tctcaacaag tggaacattt agtgatagaa tgactaaact gttaacagga
    14941 gaagatggtg aacatgcagt tttatcacgc tacaacttag cgcctgatga attagaaaaa
    15001 tataaacaac ttattttaga cgataaaatg ttagttgtag cggttcgtga caaatcatca
    15061 catcaagaag tgcatgaaaa taattcagct tatgaagaga ttgatattac acattttgct
    15121 gaagcatcta aaggtcccaa agcttagtta tcacactgaa ttcaaaatga gattactgta
    15181 ctgtctaatt taagatagta cagtttttta taccatttaa aagtttatga acagtctttt
    15241 acagcaggcg attatgctat aatattattt aaattgggga ggtgttctta tggaatttaa
    15301 aaccattcaa tctgtcaaag atccacatta caacgaagca cttaaacttt acgatactaa
    15361 gttaaattta tctttaactg aagataaacg tatcatcaaa cgctcattag aaaacaacaa
    15421 aaccgaaaac gattatgtct ttatcgtagg cttagaaaac gatgttgttg tcagtctagc
    15481 aactgcacat tatgaagcca caaccaattc agcattttta atttatttat tcgcaaaaga
    15541 cctacccaac catgatgagt taatgacgca aacattacat caaattgaac atgaactaaa
    15601 cttattatca aaccttgttc attctagaag tattaacttt ttaatgatgg aagtccctaa
    15661 agaacctgca gatttaacac aagataacgg cctcattatt gaacaccgta gacagttttt
    15721 atacgaacat caatttgaaa gacaaactga aattgattac attcatccaa ctaatgatgg
    15781 tcacactaaa aaagtggact tatttttaaa gaaaaatatt aaactgacaa aggatatcta
    15841 cggtacaagt gttaaatcaa attatatttt gaagtatgta tttgccaatg gtatcgcacg
    15901 tgaaattatt tatgctttat tagaagaaat ggatttacgt aaaccatttt aaacaaactt
    15961 gaaatcacta acaataacgt ttaaactatg tatgaagcaa gactgacgtt tcactgcccg
    16021 tagacaaacg tcagtataca taaagtatat gcagaaagga ctttaaagta tgttaactaa
    16081 agaatttgca caacgcgtag aactaagtga gaagcaagta cgtaaaattg ttcaacattt
    16141 agaagaacgt ggttaccaac taagcaagac tgaatatcgt ggtcgtgaag caactgattt
    16201 caaagaagaa gatatcgagc ttttcaaaga cattgccgac aaagtaaaac aaacaaatag
    16261 ttatgatcta gcgtttgatg aattagaaaa agaaaaagac ttcctgcaag tcattgtcaa
    16321 aaacgatgac aaaaacttac ctactaatca aaatgtcgct caactagtag aagatttacg
    16381 cctagaaatc cagaaaatgc gcgaagaacg tcacctactt ggtcaaatga tgaatcaagt
    16441 acatcagcaa caacaagaat taaaagaact tcaaaatcaa cttacatcta aaatcgattc
    16501 aaatagcgaa tccttaaaag ccatccaaac atcacaagag gctatccaag aagcgcaagc
    16561 ctctcaagca aaagcattag ctgaatcaac aaataaagtt gaaaagaatg ctgttactga
    16621 agataaagct gacagtaaag attcgaaggt tgctggagtg aatacatcaa cagacgctaa
    16681 gacggatact aaagcagaaa atgcaggcga tggcactgct actaaagtag ataaagagga
    16741 tcaaatttct gcaactgaag caattgaaaa agccagcgtt gagcagtcta aaaatgacaa
    16801 tgcagctgag acttcaaata aagaagctac agtagatgca gaagcgcaac atgatgctga
    16861 acaacaagta gcagaagccc atgcagaagc ttctaaacaa gctacttcga acgattcatt
    16921 ggaagcgaaa gcagaaaatg acagcacagc ttcacagtca gaaatgtcag aaccaaaacc
    16981 tcaagaagag aaaaaaggtt tcttcgcacg tttattcaac ctgtaaactt gtgatttgaa
    17041 cacaaattta atactcaatt catatatagc tttgatttaa aaactgattt ttataaacct
    17101 atagaactgt aatttacttt caaatttaat actcaattca taaaacctga aacaagctga
    17161 tatactccat tcaatatcta ctttgaagag agcatatcaa actaatttgt ttcaggtttt
    17221 ttaattattc attatgatta atattgaatt ttgaataaat ttttatagta tgttactttt
    17281 aaatgggatg gcttctgtgt acttctaaat agattcatct taataagtcc attcaattta
    17341 aatgtttggc tgtgttagta actgacgaat cgcacaagtg cacactcata ttcacattta
    17401 ctacaaacgc ctttttgctc aaacaaaaat atttgtttac ggtagctttt gaactaccac
    17461 ctgcacacac gaacactcaa aagatgctaa ttttgattcg acagaatttt ttacaacgtg
    17521 tttgttcgaa acgtcttttc aaggttgatc atttattatc ttttcattca ataaatatga
    17581 ggttagcaat ttttatcaaa ccgccatagc cacatctcga ttcaaggata aatttgtttt
    17641 ctcatatctt atctctcatg acagtgcacg atatatatct tttccaaata ttcatcactt
    17701 ttttcctgtt aaaatcattt ctattattaa tgctttaatg acaacttatt taatttattg
    17761 catttaatgc cctgcttcat tttcaaatat taactttaat cgactagctc atcacactat
    17821 ttaccctttc cacctatcac tactgaattc aaaacgattt gtttacaaat ccccttatgc
    17881 caacggtcga atttcatcat gtttacattt aattgcaaat gccctcttga aatattttaa
    17941 aattaatatt attcttagca tactagtcgg aattatattc aaaaatttga ggagtgacgt
    18001 tcatgaatgc ttttcttaca ctgattaata ttattgtatt ggtgattttc atcgtaatat
    18061 tgcacatgat ggctcgcaaa catatatctt ttgcgaaacg tgtgtttaca gctttaggga
    18121 ttggtatagt attcggtgtg ttattgcatc ttatttatgg cacacattcg aatgttatta
    18181 catcaacaag tgactggttc aacattgtcg gacaaggtta cgttgcattg ttgcaaatga
    18241 ttgttatgcc tttaattttc atttctattg tagctgcatt cactaaaatt caaatcggag
    18301 aaaagtttgc taaaattggt agtctcattt ttatcttttt aattggcact gtcaccattg
    18361 ctgcgattgt tggtgttgtg tatgccttgg tgtttggttt agatgcatca acaattaacc
    18421 ttggtaacgc tgaacaagca cgtggaagcg aaattgctaa acaagcgaaa gatttaacag
    18481 cacatacgtt accacaacaa attttggaat tattgcctaa aaatccattc ttagatttca
    18541 ctggacaacg tgcaacttcc acgattgctg tcgtgatatt tgcttcgttt ataggatttg
    18601 cttatttacg cgtagcacgc aaacaacctg atcatggtga attgcttaag cgtgcgattg
    18661 acgctatcta ttcactagta atggcgattg tgacgttcgt cttacgttta acgccatacg
    18721 gtgtattagc aattatggcc aacacattat cgacaagtga ctttggcgcc atttggactt
    18781 taggaaaatt cttaatcgca tcgtacgctg cattaattac gatgtatatc attcacttaa
    18841 ttattttaag cctgttaggt attagcccaa ttcgctatgt taaaaagact ttggaagtat
    18901 taatctttgc attcacatca cgctcaagcg cgggtgcctt accattgaat gtacaaacac
    18961 aaacaagacg tctaggcgtg ccagaaggta ttgccaattt cgctgcgaca tttggattgt
    19021 cgattggaca aaatggttgt gcaggcattt accctgccat gcttgcaatt atggtcgcac
    19081 ccgtcgctaa tgtagaaatt gatttacaat ttattgtaac attaatcgct gtcgtgatta
    19141 ttagttcatt cggcgttgct ggcgtcggtg gcggggctac atttgcatcc atattggtgt
    19201 tgtcaacgtt aaacttgcca gtcgctttag ccggcgtgct catctccgtt gaaccattga
    19261 ttgacatggg tagaacagca ttaaacgtga acgattcaat gctagctggt acaggtaccg
    19321 ccaagttaac taaacattgg gataaagata ctttcgattc aaatgataat gctgcattaa
    19381 cttcacacta attcattgca agaaataccc gccccctcta gctatactta tcattttaag
    19441 ctagaggggg gcgggttatt ttggtatctt gctatcgctg tattaagcct gatttttgca
    19501 attgttctaa catatctaat cttgctttgt ttcctaggaa catctcaatt tgctgcgacc
    19561 atgtttcttt gcgattcccg tttgtacgtt gatcatagta ctcgctgatt gtctggtcgt
    19621 aatctgccat ttgtgcatac tgtgtttcct tatcagcatg atacttatta tgatggaaga
    19681 catggtcaaa tggtaagcgt ggcttggctg caccattttc gtcatctgcg ggttccccta
    19741 ctgccatacc aaataccggg aagacatagt caggtaagtc taaaatttct cgaacgcgtt
    19801 caacatcatt tcttaatgat cctaaaaaga caatgccata ccccatatct tcagcagtta
    19861 ccgcaatatt ttcggcaact aatgctgcat cgattgcacc tactagcaaa ccttccgttg
    19921 aaccatatgc attttccata tcagtttcag catgttgatc aactaaatga tgacgataat
    19981 aatcaataac aaagacgaat aaatagccat tttctacaac ataaggttgt ccagaaactt
    20041 ctcgtaaatt ttctttaatc ttctcatcgt cgataccaat aattgagtat gcttgcagga
    20101 aacttgacgt cgaagcgctt tgtccagctt ctaccaattt cttaacaacg tcttcactta
    20161 aaggtttatt cttaaatttt ctaacagaat gatgcttttt cacaagatta tatacatgtt
    20221 ctgacacgtt acccactcct cgatacttta caattaattt aaaatcttca tattgcaatt
    20281 gtacacgtcc aaactttttt atagcaatta tttgcttata agttaataat tgaacttttt
    20341 aaaatattgt ctcatacttt aaaaatccag tttctcactc attaccttca tccatctcga
    20401 acaaatattc tcacaccatt atcaataagg ttgacttagt tgagaataca aatcttcttt
    20461 ttttacattt tttacattaa aatcaagata ttaaatatag acacaacctt ttaaatcatc
    20521 aactacaaaa tccattatca aag
//
LOCUS       DAKTGS010000035        20013 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.035, whole genome shotgun sequence.
ACCESSION   DAKTGS010000035 DAKTGS010000000
VERSION     DAKTGS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20013
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.035"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(92..682)
                     /locus_tag="O0286_001096"
     CDS             complement(92..682)
                     /locus_tag="O0286_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCY0169480.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(836..2098)
                     /gene="clpX"
                     /locus_tag="O0286_001097"
     CDS             complement(836..2098)
                     /gene="clpX"
                     /locus_tag="O0286_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCY0169481.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(2249..3550)
                     /locus_tag="O0286_001098"
     CDS             complement(2249..3550)
                     /locus_tag="O0286_001098"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCY0169482.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(3598..3708)
                     /locus_tag="O0286_001099"
     CDS             complement(3598..3708)
                     /locus_tag="O0286_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169483.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(3713..4642)
                     /locus_tag="O0286_001100"
     CDS             complement(3713..4642)
                     /locus_tag="O0286_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169484.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(4661..5269)
                     /locus_tag="O0286_001101"
     CDS             complement(4661..5269)
                     /locus_tag="O0286_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0169485.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(5411..5767)
                     /gene="rplT"
                     /locus_tag="O0286_001102"
     CDS             complement(5411..5767)
                     /gene="rplT"
                     /locus_tag="O0286_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCY0169486.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(5814..6014)
                     /gene="rpmI"
                     /locus_tag="O0286_001103"
     CDS             complement(5814..6014)
                     /gene="rpmI"
                     /locus_tag="O0286_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCY0169487.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(6043..6570)
                     /gene="infC"
                     /locus_tag="O0286_001104"
     CDS             complement(6043..6570)
                     /gene="infC"
                     /locus_tag="O0286_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCY0169488.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(6599..6724)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(6799..8292)
                     /locus_tag="O0286_001105"
     CDS             complement(6799..8292)
                     /locus_tag="O0286_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0169489.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(8718..10655)
                     /gene="thrS"
                     /locus_tag="O0286_001106"
     CDS             complement(8718..10655)
                     /gene="thrS"
                     /locus_tag="O0286_001106"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCY0169490.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(10853..10975)
                     /locus_tag="O0286_001107"
     CDS             complement(10853..10975)
                     /locus_tag="O0286_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169491.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(11068..11988)
                     /gene="dnaI"
                     /locus_tag="O0286_001108"
     CDS             complement(11068..11988)
                     /gene="dnaI"
                     /locus_tag="O0286_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCY0169492.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(11988..13388)
                     /locus_tag="O0286_001109"
     CDS             complement(11988..13388)
                     /locus_tag="O0286_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCY0169493.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(13389..13859)
                     /gene="nrdR"
                     /locus_tag="O0286_001110"
     CDS             complement(13389..13859)
                     /gene="nrdR"
                     /locus_tag="O0286_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCY0169494.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(14069..15094)
                     /gene="gap"
                     /locus_tag="O0286_001111"
     CDS             complement(14069..15094)
                     /gene="gap"
                     /locus_tag="O0286_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0169495.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(15264..15887)
                     /locus_tag="O0286_001112"
     CDS             complement(15264..15887)
                     /locus_tag="O0286_001112"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCY0169496.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(15903..16775)
                     /gene="mutM"
                     /locus_tag="O0286_001113"
     CDS             complement(15903..16775)
                     /gene="mutM"
                     /locus_tag="O0286_001113"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCY0169497.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(16791..19421)
                     /gene="polA"
                     /locus_tag="O0286_001114"
     CDS             complement(16791..19421)
                     /gene="polA"
                     /locus_tag="O0286_001114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCY0169498.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(19715..>20013)
                     /locus_tag="O0286_001115"
     CDS             complement(19715..>20013)
                     /locus_tag="O0286_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169499.1"
                     /translation="YKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKD
                     HPRQEVYTVESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT         6049 a         3833 c         2705 g         7426 t
ORIGIN      
        1 acgttgaaaa atgttatcaa ttgtgtacta attaaaagtt tcgtgcttac attattttta
       61 aaatttttgt ataagttttg acgtacaaaa actatgaaat atacggttca attaaattcc
      121 atatttgttg ttgtttatta ttttgaattg atgaataact tacaattgta tcgtctgggt
      181 ccatatctaa ttgtgtctta atatttttaa tatgcttttg aaccttacct tttggaattt
      241 tgtcttcttt agtgcatata actaaagtag gaatatcaaa atgtttcaaa taattgtaca
      301 ttaagatatc atcttgtgtt ggatcatgtc ttaaatcaac taattgaata actaattgca
      361 aattctctct cttagttata tattcctcaa tcattttccc aaatttttca cgttgtgttt
      421 tacttacttt agcatatcca taccctggaa catccacaaa aataagttgt tcatctatat
      481 tataaaaatt taacgtttgc gttttgccgg gttgctgtga tgtacgtgcc atattttttc
      541 tgccaatcat actattgata aatgtagact tacctacatt agatcgaccg ctcagtgcaa
      601 cttcagacaa ttctgtttct ggatattgtt cttcttttac tgcactaatg attaattcta
      661 tattattagg attaactttc attttatatc ctcacttttt tatattctct ttcaattcta
      721 ccattatata atatttgaac ctttcggtat acattataaa aaagctccga tcaaagttaa
      781 actatttgag cgtcaacttt gattggagct tttcactttt ataacacatc aatgattaag
      841 ctgatgtttt actattatta attaaattgc cttctgcgtc gtatagttct ggttcagttt
      901 cttcattaat tgtttgtgct gtaataacta ccttcgttac attttcgtta gaaggcacat
      961 caaacataat atcgattaac gattcttcta tgattgaacg taaaccacgc gcacctgttt
     1021 ttctttcaat tgctttttca ctaattgctg ataaagcttc ttcagtgaac tctaaatcca
     1081 catcatctaa ttccagcatt ttagtatatt gtttcacaag tgcattttta ggttgcgtta
     1141 agatgttttt caacgcagtt acatctaatg tttctaaatt agctacaatt ggcacacgtc
     1201 cgataaattc aggaatcaaa ccataggctt gcaaatcttc tgggcgaatt tgtgctaata
     1261 atgcttgttc gtcatattta tcagcttcat tgcttgagaa accaataact ttttcaccaa
     1321 gacggcgctt aatcacttct tcaataccat caaaggcacc accaagaata aataagatat
     1381 ttgttgtatc aatttgaatc atttcttggt ttggatgttt gcgtccacct tgtggcggaa
     1441 cacttgcagt cgtaccttct aagattttaa gcaatgcttg ttgaacacct tcacctgaaa
     1501 cgtcacgtgt tatagatgtg ttttcagatt tacgtgcaat tttatcaatt tcatctacat
     1561 aaataatacc tttttcggct ttatcaatgt caaagtcagc tgcttgaatt aatctcaaca
     1621 agatattttc aacatcatcg cctacataac cagcttcagt taaacttgtc gcatctgcaa
     1681 ttgcaaatgg tacattcaac gtcttggcta aggtttgagc taataatgtt ttaccactac
     1741 ctgttggccc aattaatgca atgttacttt tttgtaattc aacatcatct tcttttggtc
     1801 ctaattgttg aatacgctta tagtggttat aaacagctac agctaaagat tttttagctt
     1861 tttcttgacc aataacatat tcgtttaaat gatccataat ttctttagga gtaggtaatt
     1921 ctgtcatcgc ttcagaagtg ttttgagcta attcttcttc gacgatttct gagcataatt
     1981 caatacactc attacaaata tatacaccac ttcctgctac aagttttttt acttgatctt
     2041 ggtcttttcc gcagaaagag catttcaaat tttcttcatc ttcattgaat ttaaacattc
     2101 tttttacacc cctattcgtt aaagactata ctagattgga tgttacaatg caacatatta
     2161 acatacaaac tttttgctta aagaatagta gcagatacat aagctatgca tctgctactc
     2221 atttttgtaa tttaatattt aatgaagatt aatcttcttt agttccttca acgaactttg
     2281 cgttatctct taataaatcg ataacttttt ggatacgaac atcattttta atgatatcag
     2341 tattacctaa agtatttttg atatcttcaa ctgagatatt aaattgttta ctcatttttt
     2401 ctaattcttt atcgatatct tcatcagtag cttcgatttt ttcagcttca gcgatcgcag
     2461 ttaaagttaa gttagtttta acacgttgtt ctgcatcgtc tttcatttgc tctcttaatt
     2521 gagtttcatc ttgacctgag atttggaagt acgtttgtaa atctaaacct tgttgttgaa
     2581 ttctttgtgc aaattcagac accatacgat ctaattcagt attaaccatt gcttcaggaa
     2641 tatcgattgt tgtattatca gtagcttttg taatcgcttc ttctttttca acattttcag
     2701 catctgtagc tttttgttca gctaaacgtt tacgtaagtt ttctttgtac tcgtctactg
     2761 tatttgcttc tgcatctaat tcattagcaa tttcatctgt taattctggg acttctttaa
     2821 atttaatttc gttaactttt gttttgaaag ttgcttcttt accggctaat tcttcagcat
     2881 ggtattcttc tgggaatgtt acgacaacat ctttttcttc gtcaactttc ataccttcta
     2941 attgctcttc gaaaccaggt atgaatgaac ctgaaccgat ttctaaatcg taaccttcag
     3001 cttgtccacc ttcgaattct tctccgtcaa ctgaaccact aaagtcgatg ttaactgtgt
     3061 cgccattttc aacaacacca tcttctttaa cgaccatttc agctaaatgt cctaagctgt
     3121 ggtcaatcgc ttcttgtaac tcatcatcag ataattcagt ttcttgtttt tcaatttcaa
     3181 gacctttata gtctcctaat ttaacttctg gctcaactgt aactgttgct tcaaaaatga
     3241 aatctttacc tttttcaatt tgagtaacac ttacttctgg ttgtgcaact ggtttaatat
     3301 cagtttcgtc aattgcttca ccataagcat ctggtaataa aatgtcgata gcatcttgat
     3361 ataatgcttc tacaccaaag cgttgttcaa aaattggacg tggcacttta cctttacgga
     3421 atccaggtac gttaatttgt ttaaccactt ttttgaatgc ttgatctaac gctttgttta
     3481 ctttttctgc aggaacagta acagttaata aaccttcgtt accttccttt ttttcccaag
     3541 ttgctgtcat gtatatatac ctccatgatt aactaattta ttttttcaac ttccctatta
     3601 tatcataggt ctattcccta tacaaacatt gaaatcacaa cgtttatata tttgtaaatc
     3661 aacttttttc gtcaaaacta tattaaaatg ctgagtatta ttttgcaaca atttaactat
     3721 tattattcaa atctaattgc tgaataaaat taaatgcatc gatactttgt aattcacatt
     3781 taagtcctaa catatttttg aaataacatt cgtatgcatt aatccattta ttcgtttcaa
     3841 acaaagtttc aatatcaatt ggataaatca tgatagagtg attattcata atatggtgtg
     3901 cttcttcagc gatatgtaaa gcaccatcat ttaatgtttc cataacgtta ggtataactt
     3961 tttctttaag tgttgtatgt tctagccctt ttaaattagc tggtacaaaa gttacatcca
     4021 taccatactt ttcaattgtc agttcttgtg tacaatttgc gaaccttaaa tactcaatca
     4081 ttaaactaat gaggttataa ctgtacgtat tagattttaa tatatataat accgtttctt
     4141 gaaattgaaa atgaccattg tctattaact tcaaaatcaa gtgcgtctgt tccctcattg
     4201 ataacgtatc aaaatcagct aatgactgag tcaatcgttt ttcatcttca attaattttg
     4261 acttagcaaa ttctttaaga ggatgtaatg ccattcttgt tttgtgatct ttgacttcat
     4321 caataatttg atgaattact tctaccgctt caaaatattg tcccaaacca atcaaacttt
     4381 tcacgtaata aatcatcaat gcatcatatt gttgtatgcc agttttcaat aagacaattg
     4441 tttcttctct taattcaaga aatgaccccg tctcatataa catacggcat ttcatcattg
     4501 ctatttcttc agtgagttca aattgacgtt catattgcac aatatattca tacatcaggt
     4561 catagttttg gtcttgttca gcacgtttta tatctttata taactttttc gaagagttag
     4621 gaaactgaat aatatctgac atatatgtca cctcgtttga ttataaatct tgaaggatta
     4681 atgcactcca cgtttcgaaa tcatcatatg actctatgtt gcctttttca acccatttta
     4741 ttcgtaaagt atctgtttct ttagcttcta catcattcgt acttacagtg attttaaata
     4801 caacaccaat atgtaccttg cccacttcat tattatcgtc attaataaaa ccgatatatt
     4861 ccatattttg tgaatcttgc tcacttaaac ctacttcttc ttctaattct ctctgtgcat
     4921 taactctcaa tacttcgtta atagattctg ctcctggaac atcattcata tgaccgccta
     4981 cacctattga agattgtcca tgcaatcgag cttctccacc gccagataat cgttcataca
     5041 ctaatatctc gccatgctca ttttcaagta aacaataaga aatgagttgt ttatatgatg
     5101 gatcctcttc catatcgccg cgtcgcttaa cttcatattg acttagcgca tcaaaaatat
     5161 tttgaccttc tggtttattc ttatttaaaa acccattgaa agtattcttt tcattattaa
     5221 aaataatttc tctaggtact acaatgattt gttcatcaaa tttagacatc gtataaaact
     5281 cctcacttta atgtttgttc tcttaaatgc attttagcaa atatgtcata ttttagcgat
     5341 taaatgtggc attttgctat ttaaaaaata aaaaagagat gacaatgcca tctctttttt
     5401 ataagtgtta ttattttaaa gcatctttag ctttagttac taattgagca aatgcttttt
     5461 cgtcagaaat tgcgatttct gataacattt tacggttaat gtcgatacca gcttttttca
     5521 aaccgttcat taaacgtgag tagctcattt catgttgacg agctgctgcg ttgatacgtg
     5581 taatccataa tttacggaag tcacgtttac gttgacgacg gtcacggaaa gcatattgac
     5641 ctgatttcat tacttgttgc ttagctactt tgtataatgt atgttttgaa ccgaagtaac
     5701 ctttagctaa tttaatcgtt tttttacgac gcgctcttgt tactgttcca cctttaactc
     5761 gtggcataaa aaattcctcc ttaggtaatt cctaccgatt tctgtatttg ttcttatttt
     5821 ttgtatgcta ataattgttt tacacgtttc atatcgctct tagacactaa tctagcttta
     5881 cgtaattgac gtttttgttt agtgctcttg tttgcgaata agtgagatgt gaaagctctt
     5941 gaacgtttta attgacctga agcagttctt ttaacacgtt tagctgctcc gcggtgagtt
     6001 ttcatttttg gcataatata agtcctcctg taatagcttt aattattttt cagctgttgg
     6061 cgctaacatg ataaacattt gacgcccgtc cattttaggt ttttgttcaa ctgttgctat
     6121 atctttgcat tcatctgcat atttttctag cacacgttga ccaatttcct tatgcgtaat
     6181 ggcacgccct ctgaaacgaa tagatacttt acatttatcg cctttagtta agaatttacg
     6241 tccgtttttc aacttcgttt ggaaatcatg ttcctcaatt gttggactta aacgaatttc
     6301 tttaacattg ataatttttt gtttcttttt catttctttt tcttttttct gttgttcgaa
     6361 tttgaattta ccgtaatcca taattcttgc aactggtggt ttcgcattcg gtgcaacgac
     6421 cactaagtct aaatctacac gttcagccat ttctaaagct tcacgctttg atttaacacc
     6481 aatttgttca ccatcttgac cgattaaacg taattctttt gcacgaattt tgtcattgat
     6541 ttgagtttga tcttttgcta tggttgacac ctccaaaatt tttacgaaat ttgcaccaag
     6601 caaaaaggaa gagcaggtat aaaatacccg ctcttcctta tacacagtta tgtgtaatgt
     6661 gattaacctg ccaactgctt tatgcgtcgc tacaggtgag aagcgggtgc ttctacttgg
     6721 ttcgtttcgt attcaacgtt attaatcata tcaacaattc acatttaagt caacactata
     6781 actgtaatta tttttatttt aaccttttat ttcatccatt gacacgtctt gacgtaaatc
     6841 tacttgttct aatggaattt ttttcgtttt atatcgaagc ttatgataaa taaagaatgc
     6901 taaaaatact ggaattccca tatacgtaat taagaagcga ctaaaattaa aatctcctgt
     6961 tttaataaag tcaacatctt gaccaataat tactacaata cataaaaagc cagcaaataa
     7021 tggtccgaat gggaataatt tagcagtata ttttaattta gatttgtcat aattttgttt
     7081 atcaaatgct cttctaaatc gataatgact tactgctata cctacccaag caataaaacc
     7141 agttaaacca cttgctgcaa cgatatattc gtatgcacct tttgtaaggc tttgtaatac
     7201 gaaaattatc actacaatga ttgctgtaac gagtaacgac atatatggca caccgttttt
     7261 atttgtttta ccaaatgctt caaacgctaa tttatcttta ctcattgaat atagcattcg
     7321 agttgaagca tacatacctg agttacctgc tgataacaca gacgttaaaa tgactgcatt
     7381 cataaatgat gctgcaaacg caaatccagc atttttaaac actaatgtga atggagacgt
     7441 tgctacatta tcactacccc ccattaatgc actactatca taaggaatta acataccgat
     7501 aacaaaaatg gctaaaatgt aaaataataa aattctccag aatacttgtt taattgcttt
     7561 cggcacagca cgttcaggat tttctgattc accagccgta ataccaatta actcagtacc
     7621 ttggaatgag aaaccagcga ttaagaatac acctagaatt gataacaaac ttcctcctaa
     7681 gttgccacca agaattggac cttcaccttt attaaatatt tcgaatccta caacatgacc
     7741 acccatgatt ccgacaatcg ttaataaacc aattgcaatg aaaacaataa ctgtaaccac
     7801 ttttatcaat gccaaccagt attcactttc accatagacg cgaactgata acgaattcag
     7861 actaaaaatt ataactaaga acaacgcact ccatgcccaa gcgggtatgc cttgcaatgg
     7921 tgtccaatat tgaatgactt gtgctgcaat cgtaatatct gctgctacag tcactaccca
     7981 gttaaaccaa tagttccaac caagcgcaaa ccctaaagat ggatcaacaa atcttgtagc
     8041 atatgtacta aatgaacctg atactggcaa atacgtagcc atttcgccaa gtgacgtcat
     8101 taagaaaaat accattattc cgataattgc gtatcctatt aatgcaccca aagcacctgc
     8161 atcatgaatt gctccaccag aagttacaaa taaacctgta ccaatacaac ccccaatcgc
     8221 aatcatagaa atatgacgat ctttaagtcc ccttttgaca acattgttac tttcattttg
     8281 aacttttgac atataagctc cctttccatg ttttagaggc caagaaataa ataaccttta
     8341 ttcactttca tacccttttt tacagaacat agtgatatta aaagtaaaaa agtggctaca
     8401 tacctttaag atatgcaacc actttggcaa ttatctatat aaggtagcac atcacattct
     8461 gtgacagtac agttcctatt cgaaaactgt cccaatagat ataatttatg gtttatatct
     8521 atttcggcat ctttaccttt cacttgttca acttatgtac cataaatact tctgacaagt
     8581 tactaattaa acatgcaacc tctaactcaa tttaatattt taactaactt gtaatataca
     8641 ggattcatca cgcataatca accctgtaaa acttgatacg caataaaagt tttaaagcat
     8701 tttattgcga caactgtcta tctatgtttt ttcaaacgaa tttcatcaac tagattccag
     8761 ataaattcat ctttttcaac tgtttcttgg tcttgcgatc catattgacg cacattcact
     8821 tgattatttt caacttcctt atccccaact acgatttgat aaggtatttt ttgcatttga
     8881 gcttctctga ttttataacc cattttttca ttacggtcat caatacttac acgaacgcct
     8941 tgagatttca attcatcttg taattggcgc gcataatcat aatgtaaatc aacgttaact
     9001 ggaatgattt gaacttgttt tggcgctaac caagttggga atgcaccttt agtttcttca
     9061 gttaaaaatg ctacaaatcg ttccattgtt gatacaacac cacgatgaat aacaactgga
     9121 cgatgatgtt caccatcttg accaatataa gttaaatcaa aacgttctgg taataagaaa
     9181 tcaagttgtg ctgttgataa tgtctcttct ttacccatcg ctgttttaac ttgaacatct
     9241 agtttcggac catagaatgc cgcttcacca atcgcttctt cgtacgataa gccaagctca
     9301 tccgctgcct ctttaagcat attttcagct ttattccaca tatcatcatc atcaaagtac
     9361 ttttctttat cttcagggtc tctataactt aatctaaagc tataatcctc gaaaccaaag
     9421 tctttataca catcaataat catgtttaca acgcgtttga attcttcttt aatttgatca
     9481 ggtcgaacaa agatatgtga atcatttaaa gtcataccac gaacacgttg taatcctgat
     9541 acagcaccac ttgcttcata tctatgcatc gttcctagct cagcgatacg gataggtaat
     9601 tcacgatatg aatgtggttt attcgcataa atcatcatat gatgtggaca gttcattgga
     9661 cgtaatacca tagattcagt ttcatctaac tgcattggtg ggaacatatc ttcttgatag
     9721 tgatcccagt gaccagatgt tttgtataaa tcaacattag caagtactgg tgtataaacg
     9781 tggtcatatc ccatgctaac ttctttatca acaatgtaac gttcaatttc acgtctaatt
     9841 gttgcaccgt taggtaacca taatggcaaa ccagcaccaa ctaattggct atttgtgaat
     9901 agttctaact ctttaccaat tttacgatga tcacgttctt tacgctcttc taacatttgt
     9961 aaatgtgctt tcaattcttt tttatcaaag aaagcagtac cgtatatacg ttgtaacatt
    10021 ttgttgttac tatctccacg ccagtatgca cctgctgtag ataatagttt aaactcttta
    10081 attttagctg ttgatggaac gtgaactcca cgacataaat cagtaaaatc accttgacta
    10141 tataatgtta cattttcatc ttcaggaatc gcgtcgatta attctaattt gtattcatca
    10201 ttgctgaata actctttcgc ttcatctcgt gaaaccactt ttcgttcgat tttcatattt
    10261 tcgttaacga tttgtttcat tgttttttca atttgttcaa agtcatcaga tgagatgttt
    10321 tggtcaatgt cgaagtcata gtagaatcca ccttctatta caggacctac accaaattta
    10381 acattaccat ataacctttt aatcgcgtgt gccattaaat gtgcagtaga atgacgtaat
    10441 acctctaacg cttcttcact acctggtgtc acaatttcaa ttgatccatc agtttcaagc
    10501 ggtttagtta aatctacaag ttgcccgtta aatttgccgg caacagcttt tttacgtaat
    10561 ccaggactaa ttgattgtgc tatatcttca gtagtagtac ctttatcaaa cgccttttta
    10621 ttaccatctg gaaattgaat attaatttgt tccatgttaa accctcctat ttgctttata
    10681 aatactttta tctgacctcg ttaaaaaatt gaacaacaat catcgtttta acattaaacc
    10741 tattaacaat aaacagggaa tttatgacac caacaaacat ttatgtctga aaaaaacaaa
    10801 aaatcccgcc cctatacaag ggacgagatc gtcgtggtac caccctagtt atctaataca
    10861 atttatgtat taaatatctc tgcttaagat aacggtcttg atccgggtat tcattacaaa
    10921 taccataaat gaagtagtaa tcatctaatt tattaaccat attctcatca aacaatggct
    10981 ttctgtgtaa tattgattag atcatcttgt cttcaatcat ttaaacgatt tagatttgta
    11041 ttcattatac accaatcatt ttaaaattca attgtttctg aaattttctc ctgataaaaa
    11101 gtatggtgtt gacaaagatt tgacacgttc aataatacgt gctgctttag tcttctcttc
    11161 accatcacga gtcatcgcta aatgatgttc caattcacta tagtcaaaat tagaactaaa
    11221 gaatgttggt aattcatgaa ccattcgata atgtagcaaa ggtccaatta cctcatctct
    11281 cacccatgga gtcacttctt cagccccaat atcatcaagc attaaaatgt ttgcttctct
    11341 tacgcgatgt aatttctttt caaaagaacc atctttaaag ccacctttta atgttctaat
    11401 aaattccggt aaataaataa ttgtcgaacg taccttctta gatttgagct gattcgcaat
    11461 tgcacctaga ataaaagatt tacctgtccc aaatggacca taaaggtaaa ggcctttcac
    11521 ttgttcccca ttagttattg ctgtacaaat atcatctgct gccatagcta catcaagacg
    11581 gtctcgatga ttcatataaa tatctttcaa tttggcattt aaagtatctc gttgcatatg
    11641 atgagatgta attagctcag cttcaaagcg ttcttcgtcg tacttgattt tacatgggca
    11701 ttgtaaatag cgtattttaa ttcggttatt atcaacatat aactcaggca catgcccctt
    11761 tacgaaattt ggacaatcag caaatttatg accgtcataa tgtttttgtt gatctttata
    11821 ctcttgtaac acatttaagt cttcatcaat catagcattc gttaattcag ctcgatgcgc
    11881 ttccaaaaat tgcttaacat ctgggtcatt gattacttct ttttttatct tttctattct
    11941 tttttcaaag tcctgcgacg tgttaattat acttttaaat tgcttcatta ttgactgtcc
    12001 tcctcccatt ttttagataa tttatctaga aatgcttgtc gatcttgctc taattgttga
    12061 tcatctacgc tattatcttt agccgaatct tcttcactag gtttatctct attttctaac
    12121 catttaggtg ttttttcttt tgaaatacga ttacgctgcc catagtatga accacgcttt
    12181 tggtaatttc cgctagaacc ctcattttta ggttgattaa cttttttagc gtaattatat
    12241 gcttctttag ctgtcttaat accttttttc ttccaatttg atgctatttc caaaatatac
    12301 gctttaggaa gtttcatatc ttcttttaac atgacaaatt gcaacaaaat attaatgacg
    12361 ccaaaagaca ttttttcacg ttcaattaat tcttcaacca ttgtcttttg cgatatagtt
    12421 ggttctgatt cagaccaaga agctaacata tcaattggac tcgtttgttc aagtaactca
    12481 aaccattcat cactttgtgg ctttggattc acttctgaag atttgcccgc cgaagatgat
    12541 gtagcaggag atttcacctg taatttaggc atttgatttt cgtgttccat taagtaatac
    12601 gagcgtgctt gtttacgcat ttcttcaaag gataactgtt gtccacttgt aattgaattt
    12661 aaaataacat gcttcatgcc atctgctgtt aaaccatata aagtcgcgag ttgtgtaatt
    12721 aaacgctttg catctttggt aacaatgtct tgactaataa aatgtttacc taacatttgt
    12781 ctcaacattt caaagtcaaa agattcattt gataaatcga taccttggta cggttcatta
    12841 atcggaatat cacttgtatc gatatctatt tttgtagacg gcactttaaa aacatcagta
    12901 aattgtcttg ttacctgttt aaattcactc aaatcaattt gttgatactc aaagtatttc
    12961 ttcaactcat gaaatcgacg atgctcgact tcactatata aaaagattga caacattgga
    13021 tcattaaaaa ataaatgtgc tgaaggcggt tgaattaatt ggtaaacaaa ttgtgtttct
    13081 tgttcatcat gtttgacaaa cgcctttaac aatccaatcg cttcaagtaa gtccatttgt
    13141 tgtctaaact ctagtaaatt aattttaagt tcattcataa aaatataatg agaaagaatc
    13201 aatgtttcat tatgactttc tttaacgaat tgagtcataa aatgatataa acccactgct
    13261 tgcgttccaa ttagcggtgt atacagtcga ttcaatacct ctaaatgatt cgtatttaaa
    13321 tcaaagtgtt gcataacttt gaattgatcc tttggtctta agccgaattc gaaggcttgt
    13381 cgtcccattt aagcatcact ccgtttgttt tcgcttaaaa tcccttgcat cgatgctaac
    13441 aattgatcaa catctttaaa ttctttatag actgatgcaa atctaacata tgaaacttga
    13501 tcaacatgca ttaacaagtt cataacgtgt tcacctatat ctcgtgaaga cacttccgta
    13561 tgaccttcat ctcgtaattg ccattcaacc ttgttagtta tgtcttcaag ttgttgatat
    13621 ctaactggtc gtttctcaca agaacgcaca agtccattaa gtatcttttc tcttgaaaac
    13681 tgctctcttg tgccatcttt tttcacaact ataagctgac taacttcgat atgttcaaat
    13741 gtagtgaaac gtgttccaca attttcacat tctcttcgtc ttcgaatggc atttaattca
    13801 tcggcatgcc ttgaatctac aactttagat tgtgtagaat tacatttcgg gcatttcatt
    13861 acatcaccct ctttattttg attatgccta attatactat aaatctagag atgaaaaaag
    13921 aatccctcaa tttaattcat ttaaccaaat aatgaaacaa taaaaaacat tatatcgtta
    13981 cttattaagt aatttgcatg acaatattat tgtattaaaa ataaaaaacc taactccgaa
    14041 gtcagagtta ggctataaat taattgtatt aacttgcact tacagtttct tttgatgtca
    14101 aaagtgctcc aatttgctca gcaacatcta caactctatt tgaataaccc cattcattat
    14161 cataccaagc aataactttt actttattcc ctgacatgac cattgttgat tttgcatcaa
    14221 taatagctga atttggatta gtattaaaat caacagacac tagtggttga tgttcgactt
    14281 ctatgatacc ttctaaacct gcattttcaa aagcttggtt tacttcttct gcagttactt
    14341 ctttttctaa atcaacaact aaatcaacga gcgatacatt ctttgttggt acacgtaatg
    14401 ccatgccgtg taatttacct tctaattctg gtaatacttc ttttaaagct ttcgccgcac
    14461 cagtagaagt aggaataatg ctttcattac atgaacgtgc acgtcttaaa tctttatgtg
    14521 gattatcaat atttttttgg tcatttgtaa tagcgtgaac agtagtcatt aaaccattaa
    14581 ctattccaaa ctgattattt aaaacttttg caactggacc aatgcaatta gtagtacatg
    14641 aagcattact aaaaatgtca aatgcttcta tatctaattg gttatcattt acgcctttaa
    14701 ctaccatttg aacatgtcca ccttttgaag gaccagttaa caaaactttt ttggcacctg
    14761 ctttaatatg tgcgatggct ttatcaccat gattaaattt accagttgca tctatagcaa
    14821 tatcgatatc taattctttc catggcaagt tttcaggatt gcgatcagca accaatttaa
    14881 ttttatgatc tccaacttgc aatccatttt caatcggttc aacttttaga ttatattttc
    14941 catgtgtcgt atcgtaattg attaaatgtg caattgtttc gggtggataa ctagcattta
    15001 tcgctactac atttaaattt ttattttgta atgcaatacg taataccatt cttccaattc
    15061 tacccatacc attaattgca atattcgttg acattaaaag caccccttga tctttatgtg
    15121 ttatacttta tggaattagt atagcatatt atatgaaatt tgaaagcgtt ttccgttgat
    15181 ttatttatct tttttataaa aaatagtcat tacttacata catcaacgcg tacgtaaagt
    15241 aatgacttat ttatagtgta atattaatct tcttctccgt aattcggctt ttcaatataa
    15301 ccttcttctt ctaacaatct ctcaaggttt tgttttaatt caagtttatc ccctaaatta
    15361 tcgataacat gatcggccat tcggcttttt ttatcaatag aaatttggct atagacacgt
    15421 gctttcgcat cttctaatga caaattatta cgttgcatta aacgatccat ttgtatactt
    15481 tcagaagtgt atacaaccca cacttcgtct actgtatttt ccaattcatt ttcaaataat
    15541 aatggaatat ccatgattac attatatcct tgttttaaat attcttgctt ttcttcttcc
    15601 ataatatctc gcacgatagg atgtatgata gcatttaatt ctaagcgttt ttctggatga
    15661 ttaaacacta gatcacccat ataacgacga ttcatctcac cattttcatc aattgcttca
    15721 tcaccaaaga cttctcgtac ttgagctaaa cctttactcc cctttttaac agcttccctg
    15781 gctgctttat cagcatctac tactttaaaa ccgaatacgg ataagagttc tgatactgtt
    15841 gattttcctg aggcgattcc acctgttaga ccaataactt tcggcataat ttcactcttt
    15901 ctttattttt gacatactgg acaataatga ctatttcttg tcgcgatgat ttttgtttca
    15961 atttgacttc cacacacttt gcataccggc tgcttatata cattaagatg caattgcatc
    16021 tcaccagttt ttccatcagc atgacgataa tctgaaatac ttgtaccgcc atatttaata
    16081 ccttcttcta gtacttctct aacataataa aaaaccattt cttgttgttg gtgtgttaag
    16141 tcttttactt ttttatctgg taaaacacct gcacgaaaca acgcttcaca tgcgtaaata
    16201 tttccacaac ctgcgattac tttatgatcc aaaatcactt gtttgattgg tttattctta
    16261 ttagactgtt gatgaattcg atttaaataa tacgtcaatg cttcatttga aaaaggttca
    16321 ggcgctattt ctaaaaatga aggataagat gctacagacg caacatttct aatttctcca
    16381 aaacgacgta tatctgaata aattaacttt ttgtcatttg acaactcaaa aataacatgc
    16441 caatgcttac gataattagg tatcataata tcttcaagtt catctacaat gaaaaaaccg
    16501 cccgccatac ctaaatgact aattaatgta cgttgttctc gtttattatc tagctgaaaa
    16561 acgatatatt tacttcttcg ttctacattt gtaatggtat agccttccga taaagtttta
    16621 aaagtatcta attcaattcc ttttataatt gtttccttgc cttgagcttt accttcgatt
    16681 actttatccg aaaatataac gtgttcaatt ttttgattta taacgtaggg ttcaattcct
    16741 ctttttacat gttctacttc tggtaattcg ggcataccat taacctcact ttattttgca
    16801 tcataccagg ttgcaccata acttgagtct acttttaatg gaacatctaa ttgcaatgca
    16861 ttttccatta tctcttctac aaattcacta aatgaatcta cttctgactt aggtacttca
    16921 aaaattaatt catcgtgtac ttgtaataat agtttagctt gatatgttgt ctctttcatt
    16981 ttttgagcaa atttaaccat tgccagttta atgatatctg cagcactgcc ctgtattggc
    17041 gtattcatag cagtacgttc agcaaagccg cgtaaattaa agttacgact cgtaatatca
    17101 ggaatatagc gtcgacgatg tagcaatgtt tccacgtaac ctaaagcttt ggcatctttt
    17161 acaatatcag acatatattg ttttacacct gggaaactag ctaaataatc atcaatgaat
    17221 gcttttgctt ttttacgagt aatacctaaa ctttgactta aaccataatc acttatccca
    17281 taaacaattc caaagttaac tgcttttgct tgacgacgca ttaaactatc gacttgatca
    17341 gcttctacac caaatacttt catagcagtt gctgtatgaa tatcatcgcc gttgataaat
    17401 gcttctttca tactctcatc ttgtgtaatg tgtgctaata cacgcaattc aatttgagaa
    17461 taatctgctg ataatataac gctatctttt gaagttggtt taaaggcttt tcttatttta
    17521 cgcccttctt caagtctaac cggaatattt tgtaaattag gatctacact tgataaacgt
    17581 ccagtttgcg ctaaagtttg attaaaacgt gtatggatac gttgatcatc actaattacc
    17641 ttttgcaatc cttcaacata agtagactgt aacttagata attgacgata ttctaaaata
    17701 taatcaatga taggatgttc accttgcaat tgctctaaga catctacagc agtagaatat
    17761 cctgttttcg tctttttaat aacaggtaat tgtaatgtct caaacaatac aacacctaat
    17821 tgcttaggag aatttatatt aaaatcttca ccagctgcat catggatatt tcgaatcaag
    17881 acgtctaatt tttcttgaat ttctttttcc atttcttcta aatcatgaac atctgtaaat
    17941 ataccaattt cttccatttc acttaaaatc ttagctagcg gtagctctaa atcagccaag
    18001 agttctacct gattgtattc ttctaattgt ttatccatat ttggtttcgc aaagtaaatt
    18061 gcatcagtaa tagaagcaac atatggattt aaaacatcat cttcaggtac cttaaatttc
    18121 ttaccttttc catatatact cacatcgtct ttcacaaaac tttgaccgta caatgaaaca
    18181 actgattgaa catcactaat cgtacgagat ggatcaatta tataactggc caacataata
    18241 tcgaaagaaa tattttgaat atcaatcccc aatctatgtg atgctacata tgttttttta
    18301 gcatcatata cgactttttt cgaattcgga ttttctaacc atgaaactag ttcgacataa
    18361 ttatttatgt catccgcatt aattacaata tgtttctcac ctgtaaataa agagaatttt
    18421 aaaatattat ttcgcaaata gttaccaccg tctaattcga aatggatggc cgcttctttc
    18481 aatgaagtaa aatcaatatt atcaaaagac gtttcaattt caaatgtctt ttctattgca
    18541 tcttcaacgc ttgctgattg atcaatgtca gccaacaatt gtttgaattc taacttctta
    18601 aacaattcga ttttttcttg ttgttcatct tgatgagtca ttaacgtatc ttcaagtttt
    18661 acttcaatcg gactatctac attaatcgtt gctaattctt tactcattaa tgcatcttct
    18721 ttgctatttt gaagtttttc ttttaacttt ttacctgaaa tttcatctaa atgttcatag
    18781 acaccttcta ctgtgtcaaa ttggtttagc aattttattg ctgttttctc tccaacacct
    18841 gcaacacctg gtatattatc agaagtatct cccattaatc ctttcatatc aataatttga
    18901 ttaggtgtta acccgttgta tttttccgca ataaagtcag gtgtataatg atcaacatca
    18961 gtaacacctt ttttagtgta ataaatggtt acattatccg ttgcaagttg tgttaaatct
    19021 cggtctcccg taataataat tgtctgaaag cccgctttat ctgcttcttt acttaaagtt
    19081 ccgataatat catctgcctc atagttatct aattcataac gtttaatatg ataagcatct
    19141 aataattggc gaatataagg aaattgctca cttagttcag gcggcgtttt ctggcgtcca
    19201 cctttatatt cactatattt ttcatgtctg aaagtcgttt tacctgcatc aaacgctact
    19261 aaaaaatgat ttggcttttc ttcttttaaa atcttctcta gtaacattgc aaaaccatat
    19321 actgcattgg tatgaatgcc tgctttgttt gataacaaag gtaatgcata aaaagctcta
    19381 aaacttaagc tattaccatc gattaatact aatttattca caattttaac ctccagaact
    19441 aatttatata tattttagca tattcgactg tgatgtcgta attaaagaaa ctatctcata
    19501 gagtatttat acacgaccta atttcaaata aaacaagtgc ctatatagac ttagctaaaa
    19561 aataaaaaag acataatcat gattgttctt gatgagcact ccataaagtc aggttaaaag
    19621 tccaactttt gagatgcaca ttatcatttc atgattatgt ctacttttta atatttttat
    19681 gctgctattt taaattttta taggtaagaa taagttatga atcgtctgaa ccaaatggaa
    19741 tatatttaac tttggtcatt ttcggtaatt cttcataatt attaaaccac tcttggtaat
    19801 ttttattgat tggtgacgga tgatgcatat aatgttcaaa cggcaatgat tccactgtat
    19861 aaacctcttg tcgtggatga tctttaattt gttgttttaa taactgaact cttttttcat
    19921 gttcataatg aacataaata aatgcactaa gatatatcac agctaaaact agtgatgcac
    19981 ctttaataaa attaacattg attgacttat att
//
LOCUS       DAKTGS010000036        19985 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.036, whole genome shotgun sequence.
ACCESSION   DAKTGS010000036 DAKTGS010000000
VERSION     DAKTGS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19985
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.036"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            11..154
                     /gene="rpmG"
                     /locus_tag="O0286_001116"
     CDS             11..154
                     /gene="rpmG"
                     /locus_tag="O0286_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0169500.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            210..392
                     /gene="secE"
                     /locus_tag="O0286_001117"
     CDS             210..392
                     /gene="secE"
                     /locus_tag="O0286_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCY0169501.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            405..953
                     /gene="nusG"
                     /locus_tag="O0286_001118"
     CDS             405..953
                     /gene="nusG"
                     /locus_tag="O0286_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCY0169502.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            1134..1556
                     /gene="rplK"
                     /locus_tag="O0286_001119"
     CDS             1134..1556
                     /gene="rplK"
                     /locus_tag="O0286_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCY0169503.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            1764..2456
                     /gene="rplA"
                     /locus_tag="O0286_001120"
     CDS             1764..2456
                     /gene="rplA"
                     /locus_tag="O0286_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCY0169504.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    2544..2690
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2728..3228
                     /locus_tag="O0286_001121"
     CDS             2728..3228
                     /locus_tag="O0286_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCY0169505.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            3271..3639
                     /gene="rplL"
                     /locus_tag="O0286_001122"
     CDS             3271..3639
                     /gene="rplL"
                     /locus_tag="O0286_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCY0169506.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            3814..4422
                     /locus_tag="O0286_001123"
     CDS             3814..4422
                     /locus_tag="O0286_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0169507.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            4637..8188
                     /gene="rpoB"
                     /locus_tag="O0286_001124"
     CDS             4637..8188
                     /gene="rpoB"
                     /locus_tag="O0286_001124"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCY0169508.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            8352..11948
                     /gene="rpoC"
                     /locus_tag="O0286_001125"
     CDS             8352..11948
                     /gene="rpoC"
                     /locus_tag="O0286_001125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCY0169509.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            12085..12339
                     /locus_tag="O0286_001126"
     CDS             12085..12339
                     /locus_tag="O0286_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCY0169510.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            12437..12850
                     /gene="rpsL"
                     /locus_tag="O0286_001127"
     CDS             12437..12850
                     /gene="rpsL"
                     /locus_tag="O0286_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCY0169511.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            12916..13386
                     /gene="rpsG"
                     /locus_tag="O0286_001128"
     CDS             12916..13386
                     /gene="rpsG"
                     /locus_tag="O0286_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCY0169512.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            13509..15590
                     /gene="fusA"
                     /locus_tag="O0286_001129"
     CDS             13509..15590
                     /gene="fusA"
                     /locus_tag="O0286_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCY0169513.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            15807..16991
                     /gene="tuf"
                     /locus_tag="O0286_001130"
     CDS             15807..16991
                     /gene="tuf"
                     /locus_tag="O0286_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCY0169514.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(17273..18448)
                     /locus_tag="O0286_001131"
     CDS             complement(17273..18448)
                     /locus_tag="O0286_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0169515.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            18619..19806
                     /locus_tag="O0286_001132"
     CDS             18619..19806
                     /locus_tag="O0286_001132"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCY0169516.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
BASE COUNT         6929 a         3179 c         3942 g         5935 t
ORIGIN      
        1 gtgaattatt gtgagaaaaa tacctttaaa ttgtgaagct tgtggcaata gaaattataa
       61 tgttcctaag caagaaggct cggcaacaag attaacctta aagaaatatt gtccaaaatg
      121 taacgcgcac acaattcata aagaatcgaa ataaatacat tcgaaataat actttgataa
      181 tatgttcaaa ggatttggag gttgagcaga tggctaaaaa agaaagtttc tttaaaggcg
      241 ttaagtctga aatggaaaaa acaagttggc cgacgaaaga agagctattt aaatatactg
      301 taattgtagt ttctactgtt atattcttct tagtcttttt ctatgcctta gatttaggaa
      361 ttacagcatt gaaaaattta ttatttcgtt agaggagtga agacatgtct gaagaagttg
      421 gcgcaaagcg ttggtatgca gtgcatacat attctggata tgaaaataaa gttaaaaaga
      481 atttagaaaa aagagtagaa tctatgaata tgactgaaca aatctttaga gtagtcatac
      541 cggaagaaga agaaactcaa gtaaaagatg gcaaagctaa aacgactgtt aaaaaaacat
      601 tccctggata tgttttagtg gaattaatca tgacagatga atcatggtat gtggtaagaa
      661 atacaccagg cgttactggt tttgtaggtt ctgcaggtgc agggtctaag ccaaatccat
      721 tgttaccaga agaagttcgc ttcatcttaa aacaaatggg tcttaaagaa aagactatcg
      781 atgttgaact cgaagttggc gagcaagttc gtattaaatc aggtccattt gcgaatcaag
      841 ttggtgaagt tcaagaaatt gaaacagata agtttaagct aacagtatta gtagatatgt
      901 ttggccgaga aacaccagta gaagttgaat tcgatcaaat agaaaagctt taattaacaa
      961 ttaaagttat taaactaacc aaaagataaa aaagagtatt gattttttaa ttagaaaagt
     1021 gttaaaatta tgtggtcgcg cttttagagc gcccatttcg tcacgaaatg ttaagagtgg
     1081 gagggcaaaa ctgagccctg tgaccacatc acgatatcaa ggaggtgcac atcgtggcta
     1141 aaaaagtaga taaagttgtt aaattacaaa ttcctgcagg taaagcgaat ccagcaccac
     1201 cagttggtcc agcattaggt caagcaggtg tgaacatcat gggattctgt aaagagttca
     1261 atgcacgtac tcaagatcaa gcaggtttaa ttattccggt agaaatcagt gtttatgaag
     1321 atcgttcatt tacatttatt acaaaaactc caccggctcc agtattactt aaaaaagcag
     1381 ctggtattga aaaaggttca ggcgaaccaa acaaaactaa agttgctaca gtaactaaag
     1441 atcaagtacg cgaaattgct aacagcaaaa tgcaagactt aaacgctgct gacgaagaag
     1501 cagctatgcg tattatcgaa ggtactgcac gtagtatggg tatcgttgta gaataatttt
     1561 acgaatatta aatttgatta catgatttaa acgatgaagc agataacaga gataataatg
     1621 atgaattata aatataatct gaatgactag attaatgatt gatttattca taagattaat
     1681 tcttctgttg tctgctctta acttgcatat agcaagtaat gtgggaggaa attccgctaa
     1741 aaccactaaa ggaggaacta taaatggcta aaaaaggtaa aaagtatcaa gaagcagcta
     1801 gtaaagttga ccgtactcag cactacagtg ttgaagaagc aattaaatta gctaaagaaa
     1861 caagcattgc taactttgac gcttctgttg aagttgcatt ccgtttagga attgatacac
     1921 gtaaaaatga ccaacaaatc cgtggtgcag ttgtattacc aaacggaact ggtaaatcac
     1981 aaagtgtatt agtattcgct aaaggtgaca aaattgctga agctgaagca gcaggtgctg
     2041 actatgtagg tgaagcagaa tacgttcaaa aaatccaaca aggttggttc gacttcgatg
     2101 tagtagttgc tacaccagac atgatgggtg aagttggtaa attaggtcgt gtattaggac
     2161 caaaaggttt aatgccaaac cctaaaactg gaactgtaac aatggatgtt aaaaaagctg
     2221 ttgaagaaat caaagctggt aaagtagaat atcgtgctga aaaagctggt atcgtacatg
     2281 catcaattgg taaagtttca tttactgatg aacaattaat tgaaaacttc aatactttac
     2341 aagatgtatt agctaaagct aaaccatcat ctgctaaagg tacatacttc aaatctgttg
     2401 ctgtaactac aacaatgggt cctggagtta aaattgatac tgcaagtttc aaataataaa
     2461 tgatataaac aattacaggc tgaaagaaat atctttcagt ctgtaaaaat atattgacaa
     2521 taagtaattt ccaagttata ttacttattg tgattatttt acctaagaca gtaggagtta
     2581 tttataactt aaaatttatc ctgccgaggc taaaattgac ttgaacgtga tgatctatga
     2641 tctttcaagc actttttgcc gtgggtagaa agtgcttttt ttattaattt taaaaaaagc
     2701 accaaaaatt taaatggagg tgtctgaatg tctgctatca ttgaagctaa aaaacaacta
     2761 gttgatgaaa ttgctgaggt actatcaaat tcagtttcaa cagtaatcgt tgactaccgt
     2821 ggattaacag tagctgaagt tactgactta cgttcacaat tacgtgaagc tggtgttgag
     2881 tataaagtat acaaaaacac tatggtacgt cgtgcagctg aaaaagctgg tatcgaaggc
     2941 ttagatgaat tcttaacagg tcctactgct attgcaactt caagtgaaga tgctgtagct
     3001 gcagcgaaag taatttctgg atttgctaaa gatcatgaag cattagaaat taaatcaggc
     3061 gttatggaag gcaatgttat tacagcagaa gaagttaaaa ctgttggttc attaccttca
     3121 cacgatggtc ttgtatctat gcttttatca gtattacaag ctcctgtacg caacttcgct
     3181 tatgcggtta aagctattgg agaacaaaaa gaagaaaacg ctgaataatt tttagcgtaa
     3241 aaaaattaaa aataatggag gaattataaa atggctaatc atgaacaaat cattgaagcg
     3301 attaaagaaa tgtcagtatt agaattaaac gacttagtaa aagcaattga agaagaattt
     3361 ggtgtaactg cagctgctcc agtagcagta gcaggtgcag ctggtggcgc tgacgctgca
     3421 gcagaaaaaa ctgaatttga cgttgagtta acttcagctg gttcatctaa aatcaaagtt
     3481 gttaaagctg ttaaagaagc aactggttta ggattaaaag atgctaaaga attagtagac
     3541 ggagctccta aagtaatcaa agaagcttta cctaaagaag aagctgaaaa acttaaagaa
     3601 caattagaag aagttggagc tactgtagaa ttaaaataat tcaagtatct taaacttaat
     3661 aatcaaagtt ttatagcaag tattgctata atataatgat tctttgagaa gttaaaaccc
     3721 cgttattttg ataacggggt tttattcatt taaagactga gtgaaatgtt ataattataa
     3781 tgacgagtta caaagtgaag atgaggtgga ataatgagtc attattacga tgaagatcca
     3841 agtgtaatta gcaatgaaca acgtattcaa tatcaattaa accatcataa aattgattta
     3901 ataactgata acggagtgtt ttcgaaagat aaagtagatt atggttcaga tgttcttgtt
     3961 caaacttttt taaaagcgca tccacctggt ccaagtaagc gaattgccga tgttggttgt
     4021 ggttacggac caattggttt gatgattgct aaagtatcac cacatcattc aattacaatg
     4081 ctagatgtta atcacagagc gctagcctta gttgaaaaaa acaaaaaatt aaatggtatt
     4141 gataatgtga tcgtaaagga aagtgatgct ttgtctgctg tggaagacaa aagttttgat
     4201 tttattttaa ccaatccacc aataagagca gggaaagaaa ccgtgcatcg tatattcgag
     4261 caagcattac atagattaga ctcgaacggt gaactattcg ttgtaattca gaagaagcaa
     4321 ggtatgccat ctgcaaagaa aagaatgaat gaactttttg gaaatgtaga agtggtaaat
     4381 aaagataaag gatattacat tctgagaagt ataaaagctt gaaatgaaat ggatattctg
     4441 ttatagttat ataatgtaaa aatttatgtt caataagtgt gtacttttac gttaaataga
     4501 taagttaatt aagaataaat atagaatcga aaatggtgtc atcattagtg ttgccgtttt
     4561 ctttttgtct ttttattaat atgcttatgg tatttagcta aaagcggatc acataatttt
     4621 tgaggggtga atctgtttgg caggtcaagt tgtccaatat ggaagacatc gtaaacgtag
     4681 aaactacgcg agaatttcag aagtattaga attaccaaac ttaatagaaa ttcaaactaa
     4741 atcttacgag tggttcctaa gagaaggttt aatcgaaatg tttagagaca tttctccaat
     4801 tgaagatttt actggtaatt tgtcattaga gtttgtggat taccgtttag gagaaccaaa
     4861 atatgattta gaagaatcta aaaaccgtga cgctacttat gctgcacctc ttcgtgtaaa
     4921 agtgcgtcta atcattaaag aaacaggaga agttaaagaa caagaagtct ttatgggtga
     4981 tttcccatta atgactgata caggtacgtt cgttatcaat ggtgcagaac gtgtaatcgt
     5041 atctcaatta gttcgttcac catccgttta tttcaatgaa aaaatcgaca aaaatggtcg
     5101 tgaaaactat gatgcaacaa ttattccaaa ccgtggtgca tggttagaat atgaaacaga
     5161 tgctaaagat gttgtatacg tacgtattga tagaacacgt aaactaccat taacagtatt
     5221 gttacgtgca ttaggtttct caagcgacca agaaattgtt gaccttttag gtgacaatga
     5281 atatttacgt aatactttag agaaagacgg cactgaaaac actgaacaag cgttattaga
     5341 aatctatgaa cgtttacgtc caggtgaacc accaactgtt gaaaatgcta aaagtctatt
     5401 gtattcacgt ttctttgatc caaaacgcta tgacttagca agcgtgggtc gttataaaac
     5461 aaacaaaaaa ttacatttaa aacatcgttt atttaatcaa aaattagctg agccaattgt
     5521 aaatactgaa actggtgaaa ttgtagttga agaaggtaca gtgcttgatc gtcgtaaaat
     5581 cgacgaaatc atggatgtac ttgaatcaaa tgcaaacagc gaagtgtttg aattgcatgg
     5641 tagcgttata gacgagccag tagaaattca atcaattaaa gtatatgttc ctaacgatga
     5701 tgaaggtcgt acgacaactg taattggtaa tgctttccct gactcagaag ttaaatgcat
     5761 tacaccagca gatatcattg cttcaatgag ttacttcttt aacttattaa gcggtattgg
     5821 atatacagat gatattgacc atttaggtaa ccgtcgttta cgttctgtag gtgaattact
     5881 acaaaaccaa ttccgtatcg gtttatcaag aatggaaaga gttgtacgtg aaagaatgtc
     5941 aattcaagat actgagtcta tcacacctca acaattaatt aatattcgac ctgttattgc
     6001 atctattaaa gaattctttg gtagctctca attatcacaa ttcatggacc aagcaaaccc
     6061 attagctgag ttaacgcata aacgtcgtct atcagcatta ggacctggtg gtttaacacg
     6121 tgaacgtgct caaatggaag tacgtgacgt tcactactct cactatggcc gtatgtgtcc
     6181 aattgaaaca cctgagggac caaacattgg attgattaac tcattatcaa gttatgcacg
     6241 tgtaaatgaa ttcggcttta ttgaaacacc atatcgtaaa gttgatttag atacacatgc
     6301 tatcactgat caaattgact atttaacagc tgacgaagaa gatagctatg ttgtagcaca
     6361 agcaaactct aaattagatg aaaatggtcg tttcatggat gatgaagttg tatgtcgttt
     6421 ccgtggtaac aatacagtta tggctaaaga aaaaatggat tatatggatg tatcgccgaa
     6481 gcaagttgtt tcagcagcga cagcatgtat tccattctta gaaaatgatg actcaaaccg
     6541 tgcattgatg ggtgcgaaca tgcaacgtca agcagtgcct ttgatgaatc cagaagcacc
     6601 atttgttggt acaggtatgg aacacgttgc agcacgtgat tctggtgcgg ctattacagc
     6661 taagcacaga ggtcgtgttg aacatgttga atctaatgaa attcttgttc gtcgtctagt
     6721 tgaagagaac ggcgttgagc atgaaggtga attagatcgc tatccattag ctaaatttaa
     6781 acgttcaaac tcaggtacat gttacaacca acgtccaatc gttgcagttg gagatgttgt
     6841 tgagtataac gagattttag cagatggacc atctatggaa ttaggagaaa tggcattagg
     6901 tagaaacgta gtagttggtt tcatgacttg ggacggttac aactatgagg atgccgttat
     6961 catgagtgaa agacttgtga aagatgacgt gtatacttct attcatattg aagagtatga
     7021 atcagaagca cgtgatacta agttaggacc tgaagaaatc acaagagata ttcctaatgt
     7081 ttctgaaagt gcacttaaga acttagacga tcgtggtatc gtttatattg gtgcagaagt
     7141 aaaagatgga gatattttag ttggtaaagt aacgcctaaa ggtgtaactg agttaactgc
     7201 cgaagaaaga ttgttacatg caatctttgg tgaaaaagca cgtgaagtta gagatacttc
     7261 attacgtgta cctcacggcg ctggcggtat cgttcttgat gtaaaagtat tcaatcgtga
     7321 agaaggcgac gatacattat cacctggtgt aaaccaatta gtacgtgtat atatcgttca
     7381 aaaacgtaaa attcatgttg gtgataagat gtgtggtcga catggtaaca aaggtgtcat
     7441 ttctaagatt gttcctgaag aagatatgcc ttacttacca gatggacgtc cgatcgatat
     7501 catgttaaat cctcttggtg taccatctcg tatgaacatc ggacaagtat tagagctaca
     7561 cttaggtatg gctgctaaaa atcttggtat tcacgttgca tcaccagtat ttgacggtgc
     7621 aaacgatgac gatgtatggt caacaattga agaagctggt atggctcgtg atggtaaaac
     7681 tgtactttat gatggacgta caggtgaacc attcgataac cgtatttcag taggtgtaat
     7741 gtacatgttg aaacttgcgc acatggttga tgataaatta catgcgcgtt caacaggacc
     7801 atattcactt gttacacaac aaccacttgg cggtaaagcg caattcggtg gacaacgttt
     7861 tggtgagatg gaggtatggg cacttgaagc atatggtgct gcatacacat tacaagaaat
     7921 cttaacttac aaatccgatg atacagtagg acgtgtgaaa acatacgagg ctattgttaa
     7981 aggtgaaaac atctctagac caagtgttcc agaatcattc cgagtattga tgaaagaatt
     8041 acaaagttta ggtttagatg taaaagttat ggatgagcaa gataatgaaa tcgaaatgac
     8101 agacgttgat gacgatgatg ttgtagaacg caaagtagat ttacaacaaa atgatgctcc
     8161 tgaaacacaa aaagaagtta ctgattaata cgcaatttac aaaacaggca aaaagatact
     8221 aagctgaatt ttattgatga ttcagtttag tactttaagc cattttaaat aaatgcaaat
     8281 caatcaaata gcacagctaa tctaaattga aggaggtagg ctccttgatt gatgtaaata
     8341 atttccatta tatgaaaata ggattggctt cacctgaaaa aatccgttct tggtcttttg
     8401 gtgaagttaa aaaacctgaa acaatcaact accgtacatt aaaacctgaa aaagatggtc
     8461 tattctgtga aagaattttc ggacctacaa aagactggga atgtagttgt ggtaaataca
     8521 aacgtgttcg ctacaaaggc atggtctgtg acagatgtgg agttgaagta actaaatcta
     8581 aagtacgtcg tgaaagaatg ggtcacattg aacttgctgc tccagtttct cacatttggt
     8641 atttcaaagg tataccaagt cgtatgggat tattacttga catgtcacca agagcattag
     8701 aagaagttat ttactttgct tcttatgttg ttgtagatcc aggtccaact ggtttagaaa
     8761 agaaaacttt attatctgaa gctgaattca gagattatta tgataaatac ccaggtcaat
     8821 tcgttgcaaa aatgggtgca gaaggtatta aagatttact tgaagagatt gatcttgacg
     8881 aagaacttaa attgttacgc gatgagttgg aatcagctac tggtcaaaga cttactcgtg
     8941 caattaaacg tttagaagtt gttgaatcat tccgtaattc aggtaacaaa ccttcatgga
     9001 tgattttaga tgtacttcca atcatcccac cagaaattcg tccaatggtt caattagatg
     9061 gtggacgatt tgcaacaagt gacttaaacg acttataccg tcgtgtaatt aatcgaaata
     9121 atcgtttgaa acgtttatta gatttaggtg cacctggtat catcgttcaa aacgaaaaac
     9181 gtatgttaca agaagccgtt gacgctttaa ttgataatgg tcgtcgtggt cgtccagtta
     9241 ctggcccagg taaccgtcca ttaaaatctt tatctcatat gttaaaaggt aaacaaggtc
     9301 gtttccgtca aaacttactt ggtaaacgtg ttgactattc aggacgttca gttattgcag
     9361 taggtccaag cttgaaaatg taccaatgtg gtttaccaaa agaaatggca cttgaactat
     9421 ttaaaccatt cgtaatgaaa gaattagttc aacgtgaaat tgcaactaac attaaaaatg
     9481 cgaagagtaa aatcgaacgt atggatgatg aagtttggga cgtattggaa gaagtaatta
     9541 gagaacatcc tgtattactt aaccgtgcac caacacttca tagacttggt attcaagcat
     9601 ttgaaccaac tttagttgaa ggtcgtgcga ttcgtctaca tccacttgta acaacagctt
     9661 ataacgctga ctttgacggt gaccaaatgg cggttcacgt tcctttatca aaagaggcac
     9721 aagctgaagc aagaatgttg atgttagcag cacaaaacat cttgaaccct aaagatggta
     9781 aacctgtagt tacaccatca caagatatgg tacttggtaa ctattacctt actttagaaa
     9841 gaaaagatgc agtaaataca ggcgcaatct ttaataatac aaatgaagta ttaaaagcat
     9901 atgcaaatgg ctttgtacat ttacacacta gaattggtgt acatgcaagt tcgttcaata
     9961 atccaacatt tactgaagaa caaaacaaaa agattcttgc tacgtcagta ggtaaaatta
    10021 tattcaatga aatcattcca gattcatttg cttatattaa tgaacctacg caagaaaact
    10081 tagaaagaaa gacaccaaac agatatttca tcgatcctac aactttaggt gaaggtggat
    10141 taaaagaata ctttgaaaat gaagaattaa ttgaaccttt caacaaaaaa ttcttaggta
    10201 atattattgc agaagtattc aacagattta gcatcactga tacatcaatg atgttagacc
    10261 gtatgaaaga cttaggattc aaattctcat ctaaagctgg tattacagta ggtgttgctg
    10321 atatcgtagt attacctgat aagcaacaaa tacttgatga gcatgaaaaa ttagtcgaca
    10381 gaattacaaa acaattcaac cgtggtttaa tcactgaaga agaaagatat aatgcagttg
    10441 ttgaaatttg gacagatgca aaagatcaaa ttcaaggtga attgatgcaa tcacttgata
    10501 aaactaaccc aatcttcatg atgagtgatt caggtgcccg tggtaacgca tctaacttta
    10561 cacagttagc aggtatgcgt ggattgatgg ccgcaccatc tggtaagatt atcgaattac
    10621 caatcacatc ttcattccgt gaaggtttaa cagtacttga atacttcatc tcaactcacg
    10681 gtgcacgtaa aggtcttgcc gatacagcac ttaaaacagc tgactcagga tatcttactc
    10741 gtcgtcttgt tgacgtggca caagatgtta ttgttcgtga agaagactgt ggtactgata
    10801 gaggtttatt agtttctgat attaaagaag gtacagaaat gattgaacca tttatcgaac
    10861 gtattgaagg tcgttattct aaagaaacaa ttcatcatcc tgaaactgat gaaataatca
    10921 ttcgtcctga tgaattaatt acacctgaaa ttgctaagaa aattacagat gctggtattg
    10981 aacaaatgta tattcgctca gcatttactt gtaacgcacg acatggtgtt tgtgaaaaat
    11041 gttacggtaa aaaccttgct actggtgaaa aagttgaagt tggtgaagca gttggtacaa
    11101 ttgcagccca atctatcggt gaaccaggta cacagcttac aatgcgtaca ttccatacag
    11161 gtggggtagc aggtagcgat atcacacaag gtcttcctcg tattcaagag attttcgaag
    11221 cacgtaaccc taaaggtcaa gcggtaatta cggaaatcga aggtgtcgta gaagatatta
    11281 aattagcaaa agatagacaa caagaaattg ttgttaaagg tgctaatgaa acaagatcat
    11341 accttgcttc aggtacttca agaattattg tagaaatcgg tcaaccagtt caacgtggtg
    11401 aagtattaac tgaaggttct attgaaccta agaattactt atctgttgct ggattaaacg
    11461 cgactgaaag ctacttatta aaagaagtac aaaaagttta ccgtatgcaa ggtgtagaaa
    11521 tcgacgataa acacgttgag gttatggttc gacaaatgtt acgtaaagtt agaattatcg
    11581 aagcaggtga tacgaagtta ttaccaggtt cattagttga tattcataac tttacagatg
    11641 caaatagaga agcatttaaa caccgtaagc gtcctgcaac agctaaacca gtattacttg
    11701 gtattactaa agcatcactt gaaacagaaa gtttcttatc tgcagcatca ttccaagaaa
    11761 caacaagagt tcttacagat gcagcaatta aaggtaagcg tgatgactta ttaggtctta
    11821 aagaaaacgt aattattggt aagttaattc cagctggtac tggtatgaga cgttatagcg
    11881 acgtaaaata cgaaaaaaca gctaaaccag ttgcagaagt tgaatctcaa actgaagtaa
    11941 cggaataaca agtatataac agaggctaat gctttagcct cttgttattt ttatgtaaat
    12001 tatttgattt aatgttgacg aattctcttg ttcaatgtta atatattaaa ggttgatgca
    12061 agcagaactt tggaggataa attattgtct aaggaaaaag ttgcacgctt taacaaacaa
    12121 cattttgtag ttggtcttaa agaaacgctt aaagcgttaa agaaagatca agttacatct
    12181 ttgattattg ctgaagacgt tgaagtatat ttaatgactc gcgtgttaag ccaaatcaat
    12241 cagaaaaata tacctgtatc ttttttcaaa agcaaacatg ctttgggtaa acatgtaggt
    12301 attaacgtca atgcgacaat agtagcattg attaaatgag aattagtaag tgttttactt
    12361 actaaatttt atttaaccta aaaatgaacc acctggatgt gtgggattaa aaagtgaaga
    12421 gaggaggaca tatcacatgc caactattaa ccaattagta cgtaaaccaa gacaaagcaa
    12481 aatcaaaaaa tcagattctc cagctttaaa taaaggtttc aacagtaaaa agaaaaaatt
    12541 tactgactta aactcaccac aaaaacgtgg tgtatgtact cgtgtaggta caatgacacc
    12601 taaaaaacct aactcagcgt tacgtaaata tgcacgtgtg cgtttatcaa acaacatcga
    12661 aattaacgca tacatccctg gtatcggaca taacttacaa gaacacagtg ttgtacttgt
    12721 acgtggtgga cgtgtaaaag acttaccagg tgtgcgttac catattgtac gtggagcact
    12781 tgatacttca ggtgttgacg gacgtagaca aggtcgttca ttatacggaa ctaagaaacc
    12841 taaaaactaa gaatttagtt tttaattaaa tcttaaactt aaaatattta atataaggaa
    12901 gggaggattt acattatgcc tcgtaaagga tcagtaccta aaagagacgt attaccagat
    12961 ccaattcata actctaagtt agtaactaaa ttaattaaca aaattatgtt agatggtaaa
    13021 cgtggaacag cacaaagaat tctttattca gcattcgacc tagttgaaca acgcagtggt
    13081 cgtgatgcat tagaagtatt cgaagaagca atcaacaaca ttatgccagt attagaagtt
    13141 aaagctcgtc gcgtaggtgg ttctaactat caagtaccag tagaagttcg tccagagcgt
    13201 cgtactactt taggtttacg ttggttagtt aactatgcac gtcttcgtgg tgaaaaaacg
    13261 atggaagatc gtttagctaa cgaaatttta gatgcagcaa ataatacagg tggtgccgtt
    13321 aagaaacgtg aggacactca caaaatggct gaagcaaaca aagcatttgc tcactaccgt
    13381 tggtaagata aaagctttta ccctgagtgt gttctatatt aatgaatttt cattaagcgt
    13441 tcatgcttag ggcatcgcca tatctatcgt atttattcag taatataaac tggaaggaga
    13501 aaaaatacat ggctagagaa ttttcattag aaaaaactcg taatatcggt atcatggctc
    13561 acattgatgc tggtaaaacg actacgactg aacgtattct ttattacact ggccgtatcc
    13621 acaaaattgg tgaaacacac gaaggtgctt cacaaatgga ctggatggag caagaacaag
    13681 accgtggtat tactatcaca tctgctgcaa caacagcagc ttgggaaggt caccgtgtaa
    13741 acattatcga tacacctgga cacgtagact tcactgtaga agttgaacgt tcattacgtg
    13801 tacttgacgg agcagttaca gtacttgatg cacaatcagg tgttgaacct caaactgaaa
    13861 cagtttggcg tcaggctaca acttatggtg ttccacgtat cgtatttgta aacaaaatgg
    13921 acaaattagg tgctaacttc gaatactctg taagtacatt acatgatcgt ttacaagcta
    13981 acgctgctcc aatccaatta ccaattggtg cggaagacga attcgaagca atcattgact
    14041 tagttgaaat gaaatgtttc aaatatacaa atgatttagg tactgaaatt gaagaaattg
    14101 aaattcccga agaccactta gatagagctg aagaagctcg tgctagctta atcgaagcag
    14161 ttgcagaaac tagcgacgaa ttaatggaaa aatatcttgg tgacgaagaa atttcagttt
    14221 ctgaattaaa agaagctatc cgccaagcta ctactaacgt agaattctac ccagtacttt
    14281 gtggtacagc tttcaaaaac aaaggtgttc aattaatgct tgacgctgta attgattact
    14341 taccttcacc actagacgtt aaaccaatta ttggtcaccg tgctagcaac cctgaagaag
    14401 aagtaatcgc gaaagcagac gattcagctg aattcgctgc attagcgttc aaagttatga
    14461 ctgaccctta tgttggtaaa ttaacattct tccgtgtgta ttcaggtaca atgacatctg
    14521 gttcatacgt taagaactct actaaaggta aacgtgaacg tgtaggtcgt ttattacaaa
    14581 tgcacgctaa ctcacgtcaa gaaatcgata ctgtatactc tggagatatc gctgctgcgg
    14641 taggtcttaa agatacaggt actggtgata ctttatgtgg tgagaaaaat gacattatct
    14701 tggaatcaat ggaattccca gagccagtta ttcacttatc agtagagcca aaatctaaag
    14761 ctgaccaaga taaaatgact caagctttag ttaaattaca agaagaagac ccaacattcc
    14821 atgcacacac tgacgaagaa actggacaag ttatcatcgg tggtatgggt gagcttcact
    14881 tagacatctt agtagaccgt atgaagaaag aattcaacgt tgaatgtaac gtaggtgctc
    14941 caatggtttc atatcgtgaa acattcaaat catctgcaca agttcaaggt aaattctctc
    15001 gtcaatctgg tggtcgtggt caatacggtg atgttcacat tgaattcaca ccaaacgaaa
    15061 caggcgcagg tttcgaattc gaaaacgcta tcgttggtgg tgtagttcct cgtgaataca
    15121 ttccatcagt agaagctggt cttaaagatg ctatggaaaa tggtgtttta gcaggttatc
    15181 ctttaattga tgttaaagct aaattatatg atggttcata ccatgatgtc gattcatctg
    15241 aaatggcctt caaaattgct gcatcattag cacttaaaga agctgctaaa aaatgtgatc
    15301 ctgtaatctt agaaccaatg atgaaagtaa ctattgaaat gcctgaagag tacatgggtg
    15361 atatcatggg tgacgtaaca tctcgtcgtg gacgtgttga tggtatggaa cctcgtggta
    15421 atgcacaagt tgttaatgct tatgtaccac tttcagaaat gttcggttat gcaacatcat
    15481 tacgttcaaa cactcaaggt cgcggtactt acactatgta cttcgatcac tatgctgaag
    15541 ttccaaaatc aatcgctgaa gatattatca agaaaaataa aggtgaataa tataacttgt
    15601 tttgactagc tagcctaggt taaaatacaa ggtgagctta aatgtaagct atcatcttta
    15661 tagtttgatt ttttggggtg aatgcattat aaaagaattg taaaattctt tttgcatcgc
    15721 tataaataat ttctcatgat ggtgagaaac tatcatgaga gataaattta aatattattt
    15781 ttaattagaa taggagagat tttataatgg caaaagaaaa attcgatcgt tctaaagaac
    15841 atgccaatat cggtactatc ggtcacgttg accatggtaa aacaacatta acagcagcaa
    15901 tcgctactgt attagcaaaa aatggtgact cagttgcaca atcatatgac atgattgaca
    15961 acgctccaga agaaaaagaa cgtggtatca caatcaatac ttctcacatt gagtaccaaa
    16021 ctgacaaacg tcactacgct cacgttgact gcccaggaca cgctgactac gttaaaaaca
    16081 tgatcactgg tgctgctcaa atggacggcg gtatcttagt agtatctgct gctgacggtc
    16141 caatgccaca aactcgtgaa cacattcttt tatcacgtaa cgttggtgta ccagcattag
    16201 tagtattctt aaacaaagtt gacatggttg acgatgaaga attattagaa ttagtagaaa
    16261 tggaagttcg tgacttatta agcgaatatg acttcccagg tgacgatgta cctgtaatcg
    16321 ctggttcagc attaaaagct ttagaaggcg atgctcaata cgaagaaaaa atcttagaat
    16381 taatggaagc tgtagatact tacattccaa ctccagaacg tgattctgac aaaccattca
    16441 tgatgccagt tgaggacgta ttctcaatca ctggtcgtgg tactgttgct acaggccgtg
    16501 ttgaacgtgg tcaaatcaaa gttggtgaag aagttgaaat catcggttta catgacacat
    16561 ctaaaacaac tgttacaggt gttgaaatgt tccgtaaatt attagactac gctgaagctg
    16621 gtgacaacat tggtgcatta ttacgtggtg ttgctcgtga agacgtacaa cgtggtcaag
    16681 tattagctgc tcctggttca attacaccac atactgaatt caaagcagaa gtatacgtat
    16741 tatcaaaaga cgaaggtgga cgtcacactc cattcttctc aaactatcgt ccacaattct
    16801 atttccgtac tactgacgta actggtgttg ttcacttacc agaaggtact gaaatggtaa
    16861 tgcctggtga taacgttgaa atgacagtag aattaatcgc tccaatcgcg attgaagacg
    16921 gtactcgttt ctcaatccgt gaaggtggac gtactgtagg atcaggcgtt gttactgaaa
    16981 tcattaaata atttctaatt tcttagattt tatataaaaa gaagatccct caatcgaggg
    17041 gtcttttttt aatgtgtaaa ttttgtaatg gctattcgat ttagaagaac aataattgat
    17101 gaaagactga ctaataaaac ttataactga taatactgtt taaataaaat tgttgagtct
    17161 tggacattgt aaaatgctcc cttcaaagtt ttcatttttt caatgtctac tttgaaggga
    17221 gcatttcatt agtttatgtc tcagattcat atctttcaat taatttaaat gcttaatttg
    17281 ttttaaatac ttgctctaat tctatgattt ttaaaaatac agctacagcg tattttaatg
    17341 atttttcatc aatatcaaat ttgggattat ggtgtggcgc tgtaatacct ttactttcat
    17401 taccacaacc agtcagaaag aatgcacctg gtcgtacttt caaataatgt gaaaaatctt
    17461 ctccaatcat cattaaatct gattcattaa agcgtacatg taagtcattt gttgcttctt
    17521 taataacttg atatgctttc tcgttattat ggacaggcaa atacccttta atataattca
    17581 aatcatagtt aatatcattt gctattgcta aaccttgtag aagcttatcc attttgtcca
    17641 ttacatgatt ctgtatatct gaatcgaaag ttctaactgt acctttacaa aatgcttgat
    17701 caggaataac gctatctgtg gtgcctgctt gaatcattcc aaatgaaagt acagcttgtt
    17761 taactggatc gatcgtacgt gaaattattt tttgtgcact taaaatgaac tctgccatga
    17821 ttactattgg gtcaatggtt tcatgaggtt tggcaccatg accaccacga cctttaaatg
    17881 tgacgctaaa ttcatctgga gaggccatga ttgcccccgc acgtgaatga atagttccag
    17941 taggataacc actccataaa tgtgtaccgt aaattctatc tacattttcc agacatccag
    18001 catctatcat ttcttgagaa ccacctggca tgatttcttc accgtactgg aatattaata
    18061 caacattacc ttctaataaa tgtttatgtt catctaaaat ctctgctaca gtaagtaaaa
    18121 ttgctgtatg accatcatgc ccacacgcat gcatacatcc tggattttta gacttataag
    18181 gcacatcgtt taattcctcg acaggtaacg catcaaagtc agctcttaat gcaatggtag
    18241 gtcctgtgcc caagccttta aatgtggctt tgataccatt gcggccgata ggagtttcaa
    18301 tatcacaaga taactggctt aattggttaa caatataatc atgtgtttga aattcttcaa
    18361 aagataactc aggatattgg tgtaaataac gtctgagttg aattgtttta ttttctttat
    18421 tatttgctag ttggaaccaa tctaacaccc ttatcactac tttctaaaat aatgtttata
    18481 gtataacatt ttatgaaatt atcgtactaa atgattgctt tgagatattt tatctatgaa
    18541 tgataaggct ttcaagttat gtagaattac tgtatgataa aggtattacc aaacaatact
    18601 taagggggat tatatactgt ggttcaatca ttacatgagt ttttagagga aaatataaat
    18661 tatctaaaag aaaatggttt gtataatgaa atagatacaa ttgaaggtgc aaacggacca
    18721 gaaatcaaaa tcaatgggaa atcatacatt aacttatctt caaataatta tttaggacta
    18781 gcaacaaatg aagatttgaa atcagctgca aaagcagcta ttgatacaca tggtgtaggt
    18841 gcaggcgctg ttcgtacaat caatggtaca ttagatttac acgacgaatt agaagaaaca
    18901 ctagcaaaat ttaaaggaac agaagctgca atagcttatc aatcaggatt taattgtaat
    18961 atggctgcta tttcagctgt catgaataaa aatgatgcta ttttatcaga tgagcttaat
    19021 catgcatcaa ttattgatgg atgtcgctta tctaaagcta aaattattcg agttaaccat
    19081 tcagacatgg atgatttacg tgcgaaagca aaagaagcag ttgaatcagg tcaatacaat
    19141 aaagtgatgt atatcactga tggcgttttt agtatggatg gtgatgtggc taaattacct
    19201 gaaattgtag aaattgcaga agaatttggt ttattaactt atgttgacga cgctcatggt
    19261 tcaggtgtta tgggtaaagg cgctggtacg gttaaacatt ttggtttaca agataaaatc
    19321 gatttccaaa taggtacgct ttctaaagca attggtgtcg ttggcggtta tgtagcaggt
    19381 acaaaagagt taatagattg gttaaaagca caatcacgac cattcttatt ctctacatca
    19441 ttagcacctg gggataccaa agcaataact gaagcagtta aaaagttaat ggattcaact
    19501 gaattacatg ataaattatg gaacaatgca caatatttaa aaaatggatt gtcaaaatta
    19561 ggatatgata caggtgagtc agaaactcca attacaccag taattattgg tgatgaaaaa
    19621 acaactcaag aatttagtaa gcgtttaaaa gacgaaggtg tctatgtgaa atctatcgtt
    19681 ttcccaacag taccaagagg tacaggacgt gtaagaaata tgcctacagc tgcacataca
    19741 aaagacatgt tagatgaagc aattgcggct tatgaaaaag taggaaaaga aatgaagttg
    19801 atttaatatt tatttattcc cacggcaaat attgtcgtgg gcttttttta atgtttagtt
    19861 tattaacagt aagttcgtat atcaatgttt agtgctcccc aaaattgaag tttgaatttt
    19921 aaaagcatct tgtagaattt agttgtattt ttttcaaaga aattcatttt gattattttt
    19981 gataa
//
LOCUS       DAKTGS010000037        19638 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.037, whole genome shotgun sequence.
ACCESSION   DAKTGS010000037 DAKTGS010000000
VERSION     DAKTGS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.037"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            270..917
                     /locus_tag="O0286_001133"
     CDS             270..917
                     /locus_tag="O0286_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCY0169517.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            1148..2047
                     /locus_tag="O0286_001134"
     CDS             1148..2047
                     /locus_tag="O0286_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169518.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            2040..3272
                     /locus_tag="O0286_001135"
     CDS             2040..3272
                     /locus_tag="O0286_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169519.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            3256..3441
                     /locus_tag="O0286_001136"
     CDS             3256..3441
                     /locus_tag="O0286_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169520.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            3490..3816
                     /locus_tag="O0286_001137"
     CDS             3490..3816
                     /locus_tag="O0286_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCY0169521.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            3846..4865
                     /locus_tag="O0286_001138"
     CDS             3846..4865
                     /locus_tag="O0286_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCY0169522.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(5101..5754)
                     /locus_tag="O0286_001139"
     CDS             complement(5101..5754)
                     /locus_tag="O0286_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCY0169523.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            5928..6614
                     /locus_tag="O0286_001140"
     CDS             5928..6614
                     /locus_tag="O0286_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCY0169524.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(6680..7939)
                     /gene="sdpC"
                     /locus_tag="O0286_001141"
     CDS             complement(6680..7939)
                     /gene="sdpC"
                     /locus_tag="O0286_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCY0169525.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            8229..9164
                     /locus_tag="O0286_001142"
     CDS             8229..9164
                     /locus_tag="O0286_001142"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCY0169526.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(9420..9839)
                     /locus_tag="O0286_001143"
     CDS             complement(9420..9839)
                     /locus_tag="O0286_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0169527.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(9961..10845)
                     /locus_tag="O0286_001144"
     CDS             complement(9961..10845)
                     /locus_tag="O0286_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0169528.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            11005..12666
                     /gene="hutU"
                     /locus_tag="O0286_001145"
     CDS             11005..12666
                     /gene="hutU"
                     /locus_tag="O0286_001145"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCY0169529.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            12666..13904
                     /locus_tag="O0286_001146"
     CDS             12666..13904
                     /locus_tag="O0286_001146"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCY0169530.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            14181..15302
                     /locus_tag="O0286_001147"
     CDS             14181..15302
                     /locus_tag="O0286_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCY0169531.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            15413..16294
                     /locus_tag="O0286_001148"
     CDS             15413..16294
                     /locus_tag="O0286_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0169532.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            16781..17350
                     /locus_tag="O0286_001149"
     CDS             16781..17350
                     /locus_tag="O0286_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169533.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      17403..17498
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            17575..18888
                     /locus_tag="O0286_001150"
     CDS             17575..18888
                     /locus_tag="O0286_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCY0169534.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            19055..19561
                     /locus_tag="O0286_001151"
     CDS             19055..19561
                     /locus_tag="O0286_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0169535.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
BASE COUNT         6668 a         2725 c         3868 g         6377 t
ORIGIN      
        1 tattttattt gcatagattg agactctttt aaacgtgaac attgcagtat gacatatttt
       61 tagtatagtt aacgaagcgt tgactttcaa atgagtctcc atatagatgt gtacttgtag
      121 cgggttttca ttttactagt gatgaaatat aaagttcatt ttccaaagtg attgacgatt
      181 gaaatgaagt ggcttgataa tgaatcttgt aaattataac ggtaacaata attaaaaata
      241 taaatatgat taacgaaagg gagattacaa tggaacaatt acctcaagta ggttttatga
      301 aatcattcgt tttattttgg aaaaattatg taaattttaa ggggagatca agacgtagtg
      361 aatattggta tatggcacta tggcatttga tacttatggt tccagctgtg tgtgtgctaa
      421 taataaacct atttctattc tttatttcta tagcggcgga taacgaaaca ttaatagtta
      481 tgagtatatt gatgattatc cttacatctg tgtatacttt tgtctatagt ttggcaacgt
      541 ttattccaaa tttagcgttg tgcgttcgaa gatttcacga tatatcaaga acaatgttat
      601 ttccaatgat tatgacagta ttcagtatta tattttatat agtattacag attatcgata
      661 gttattatga cagtgatttt acattaatgc cgatgggtct taatattttg ttagtacttc
      721 tttattttgt ttatttaggc ctaacaattg ttatgatagt attcctatgc tttgacagta
      781 agccggcaaa taaatatggc gaaagcccaa aatacccgtc tacaattaaa aatcaaccag
      841 tgacatctga aacaccggaa aaagcgtgta catctgaaac accgaaaaca ccggaagaaa
      901 cagataatca attgtaatga tataaatatc tcggcacaat gatgctggga tattttttta
      961 caaataaggc aataaaggca tggaaaaaac atatctagtg ctaatgatac ttaacatgca
     1021 aaaatggcac tatttaaatt tttcaatttc agaaatttta aaaataaatt cattttaaaa
     1081 atattgcatt tatagagaat ggaaatgtta tattaagata aatacatatt tttttaggag
     1141 gcaaagtatg ggacttgtaa ttgaacatgt tacgaaacgt tttggcaaga tgacggcagt
     1201 aaatgatatt tcattaaagt tagaatcagg taaaatgtta ggttttctag gaagaaatgg
     1261 tgcaggtaaa acaacgacat ttcgtatgat tttaggctta agtgaaccaa ctgaaggaca
     1321 cattacgtat aacggtaaaa aactagataa aacaatgtac aatcgtattg gttatttacc
     1381 ggaagaacgc ggtttacatg ggaaattgac agttgaagaa gaattgaaat atttggcaac
     1441 tttaaaggga atgtcaaaaa cagaaataca acagcaaata tcgtattggc ttgagcgttt
     1501 tgatattact gaaaaccgca aaaaacgaat tgatagtcta tcaaaaggga atcaacaaaa
     1561 aattcagctg ttagcaagta tgttacataa accagaatta ctaatattag atgagccttt
     1621 cagtggtcta gacccagtta atgttgagtt attaaaagaa gcagttaaag acttaaatga
     1681 ttggggcagt acaatcgttt atagttctca ccgtatggag catgttgaag aattatgtga
     1741 tgatgtttgt attttagata aaggtcaact tgttgtttct ggtgatatca atcatgtacg
     1801 tgcatctaat ggtaacaaga aagttgtcat tgaatcagaa acaacactac cagatttaac
     1861 aaacattaga ggcatcattc atagtgaaaa catgaagcaa ggtcttcagt tgacgattga
     1921 gaatgaagac gtagccaaag atatttatca agttgtggca catcaaggtt atgttaaacg
     1981 attccaagtt gtagaaccat cattacaaga tatattcatt gagaaggttg gtggcaagga
     2041 tgcataagtt ttgggcgaca tttactttga catatatcaa aaaaattaaa gctaaatcat
     2101 ttgtaatttt tatgattatt attgctgcct taatgattgg cctatctaac attgataaga
     2161 ttattaacat gtttgatgat ggtccagata aaattggtgt agcagcaccg aatgaacaaa
     2221 tctataaggt tttcaagcaa caggcgaata catttcatag tgatgctaag tttactaaag
     2281 tttctataga agatgctgaa aaagaagtaa aaaaacataa acttgataaa gcttatatta
     2341 tcaaggtgaa tcaaaatcgc acgttacaag ggacaatcat tagtgagaag cgagtaagtc
     2401 atgaagattc acaaaaagta caagcactgc ttacagctat tcaaacaaat atggtagccg
     2461 gggaattaaa tatcaacaaa gaagacttac aaaaattaca agcacaaagt aaagttgata
     2521 ataaggtgat ttctaatgat gaagtcgata aagtatcaga aggacaaaag attttcaact
     2581 atgctttagc ttatggcatt atctttctaa tgttcttcat tgttttaaat tatgcatctc
     2641 aaatagctat ggaaattgct agtgagaaaa catcgcgtgt cattgaaatg attattacaa
     2701 gtatttcacc aatccaacat atttttgcta aaattttagg cgttatagca gttgccgtga
     2761 ctcagattat tttaattgta ttgatggcag ttatatgcat ttttgccttt gacttaaaag
     2821 acttgctaca aggttttaac gtggaaatga atcaattgtc ttggcaaatt attattgtcg
     2881 gaattataag tagtattgtt ggaattacgg cgtatgtatt acttgcagcc atcctcggat
     2941 cattaacatc tagaatagaa gatttaaatc aatctttgat gccactgaca ttacttggta
     3001 tgattgcatt ttatatcgcg atattcacga taaataatcc tgatggacaa cttgctttaa
     3061 tcacaagtta tattccgttt ttagcgccat ttcaattagt tgtacgcgct caaacttcag
     3121 ggcttcaaat atatgaggtc atcttaagtt ctttaatctc aattgtaatg gtagctgtct
     3181 tgatatggat tgcaattaaa acatataaag attcagtact tacatttgaa cgtggcttat
     3241 ttaattcaat gaaacgtgta tttaaaaaat aaaattgatg agagtgacac acatttatca
     3301 tataaaatga tgatgaaagc gtgttactct tattttttta tatttagcta tagaataatg
     3361 tctaaaaaag tgtacaatat gaatgtgaaa acgaactttt taaaatggga atgtgaagta
     3421 agaagaaatg taaacaaatg atatgtagcc gatagattat tgatagcaaa tgtgtgatgg
     3481 agtgatttca tgttatatcc gatttttata tttatattag ctggcttatg tgaaataggt
     3541 gggggatacc tgatttggct gtggcttagg gaaggacagt cttcacttgt tgggctaata
     3601 ggcggtgcga tactcatgtt atatggtgtc attgcgacat ttcaatcatt tccatcattc
     3661 ggaagagtat atgcagcata tggtggcgta tttatcatca tgagtttgat ttttgcgatg
     3721 gtcgtagata aacaaatgcc agataaatat gatgttatcg gtgcaatcat ttgtattgta
     3781 ggtgttttag taatgttgct acccagcaga gcttgaacac ttgtggttag aagggagaag
     3841 taaaaatgat agttgaagtt gagcaccaaa gacaaggtgt agatttaatc aaaattgata
     3901 atgatgaaac taaaattatt tttacgaatt atggagcaag agtagtgtct tggaaatatc
     3961 atgataataa tattgttctt ggaaatgttg ttgaagcgga tgaattttat catagtaatc
     4021 cttttaaatt tggtgcttct ataggaagat atagcggtag aattgataat gctaaattta
     4081 agttgaaagg taaagaatat cagttagaaa aaaacaacgg tgaacatcat ttacatggtg
     4141 gatgtcatgg tttggacaat aagttatttg attatgaaat tagaaatgaa attgctcaaa
     4201 ttaaagttat atttaagacg gtgttgaaat cagctgatga tcatttcccg ggtgatatcg
     4261 atgtgacgat tacacatatt tatgatgcgg atcaccaatg gtcgattgaa tacgaagcag
     4321 ttgcatcaga agacacattg tttagtccta cgaatcatgt gtacttcaat ttgaatcgag
     4381 ataataatgt cgttgataat catcgcattt caagtaatca attagatatg tatgtattag
     4441 atgagcgaaa tattgttaca ggtgatattc tagatttaca tgaagtgttt gaagataaca
     4501 agataaaatt gagtgatatt tttacatcac aacatgcgca attaagccaa caaatgacac
     4561 gttttggtgg tctcgatcac ccatttacag ttggtgaaca taaaatgtat gttgaaaatc
     4621 atgaatttat gttagaggtc gatacagata tgcctcatgt tgtgttcttt acttttaacc
     4681 agcctgatga gtgggatagt ccatttaata tttataaacc acattctggc tttacattag
     4741 aaacacaatt tttaccaaat gatattaatt tatatggtga aaaagcaccg tcaattttaa
     4801 aagctggtga agtgttcaat tcgaaaacaa gtttccgtat cgaagaaaaa gtagaaaatg
     4861 attaaaagta tcggcattta tataatattt atgttatttg gcggaatttt gaataggtta
     4921 tcaagaatcg tatcaaatga agtgacatca ttaatataca gtttgaaata aagaaaatat
     4981 ataacataaa aaaggaatca atagcatcag tggttataga taagactatt atacaaacga
     5041 tgtgtaagtt gattcctttt tgttttgaaa agttcattta acacttataa tgaaaggtaa
     5101 ttatttcaat gttctgttat acattttttg aagtttatcg cgtcttgttg gtgacagaaa
     5161 tggattgttt agtgcatatc gtaacatatc ttgattttgt ctatcattat aataaaggtc
     5221 attaagctgt tgtacagata aacgatgttc gattggtgct tcttgtatta agtaaagctg
     5281 atcatttgga gatacatagc cttgttttaa tacccggaaa tagaaacctg ttttaccaga
     5341 tgtagacatg cgcttcacta aatcaggaat attatatttt gcttgaattt tccagcatgg
     5401 ttctctaatt tcagaaacct caattaacgc ttcacctaaa cgatattgat taccaaagta
     5461 aacatcagat tcgtctaaat aatcaaatgt taaattctct ccaaacatcg catgagtagg
     5521 tagtgtaggt aaatcatctt gatacagtgc ataattatgc ttactaaacc cacaaactgc
     5581 tttatgtggt ccaccatgat ctttataagc ctgttcatca ccagtgaaac cagttgaaga
     5641 taaccatgtt gtttgtgaaa tcttagtttt atctaaagca gagcgcatcg gtcttttcga
     5701 gctataaggc aaatcttgga tttttcctgt cgaaattgcg tgaattgata tcatatcatt
     5761 ctccttaacg agtattattt aaaatatatt gtactataaa acatttttag aattgtaaat
     5821 tctgattgat atgtttaaaa aaatgttaga aataagggta tgttaattta tacgagtcat
     5881 gtaaaatatg aattgaatag attacataaa tatgggaggc taccttcatg aaagatgtca
     5941 aagcactcaa gttaatgaca ttaaatgatg ttttaagtca aatcaatggt gacatgacgc
     6001 tgggaattgg tacaggaagt acaatggaat tactcttacc tcaaatggcg caactaatta
     6061 aagaacgtgg ttacaatatt acaggtgtat gcacttctaa taaaattgca tttttagcaa
     6121 aagaattagg tattaaaata tgtgaaatca atgatgttga tcatatcgac ttagcaattg
     6181 atggtgctga tgaagtagat ccatctttaa atataattaa aggtggcggt ggtgcgctgt
     6241 tcagagagaa agttatagat gaaatggcat cgcgttttgt tgtggttgtc gatgaaacga
     6301 aaatagttca atacttaggt gagacgttta agttaccagt agaggtggat aaatttaact
     6361 ggtatcacat attacgtaaa attgaatcat acgctgatat aaaagtagaa cgtcgtgtaa
     6421 acgaagatgt cgcatttata actgataatg gcaattatat tttagattgt aagttaccaa
     6481 aaggaattga cccatataag ttccatgagt atttaattca tctgacaggt gtgtttgaga
     6541 caggatactt cttagatatg gcagatcaag ttattgtagg cactcaagag ggtgttaaaa
     6601 ttttagaaaa ataatatata gtatagaaaa gcgcttgcta gcatcatctt tttaagaggt
     6661 actagcaagc gctttgttat tatttgtttt tatctgaaga ttgttcttca gttttttcga
     6721 tatgtttttc tgcatcaatt acaactgatg aacgtctgtc ttctttataa gtcgcaggtt
     6781 cttttaatga ttgctctatt tcatcttcat ttgaatcgtt tgttaaatcg tcactttgtg
     6841 gtgcttgttt aactgattct gctgattcta ctgactccga agtgatgtca tgatcttcat
     6901 catgattaga ttcagtttga ttacctatgt gatgttgagg ttctgttttt tcagtacctt
     6961 catgaatgct tggttcgtca gccactgtat tttcagtatc atttttagca acaccaaccg
     7021 ttgatgcagt agctacacct gttttttcag catctttaat atttgaagaa gatttttcag
     7081 cctcagtatg attagtttct tcatcgtcat tgacatggtc taaataatta ttaggctcaa
     7141 gttcttcaag gtttcgtctt gttgttaaat atgcaatacc tcggataatt aagcttaaac
     7201 caatgtatcc tactgcaacg attgctgttg aaatggcgat gactttgatt gaaaatagat
     7261 cgccgatttc ttcgctaaac aagaaaataa gtccgaatgt cattgaagca tggaatgtcg
     7321 ttgcaatata aattgtacgt cctttagtcg ctctaattaa ttcaccaaga atcatagaga
     7381 atgagaatgt ataaaggaag ttataagcag caaattctgt accataagtt gtatttgctg
     7441 agaaaactga atacatcaaa ccaacaacaa tacttgcaaa gaacgtgttc attttcgttt
     7501 caacaatatt ttgtaagtat gaacggaatc cgaattctac tacgaacgcc atcagaatat
     7561 gtccaatcag aatgtgtgta ataggtactg ataagcctgt tgattgtaat aaaataaagc
     7621 tatctgcaaa tgtattaaag ctgtacatac caataattag aataattaaa ggtaaaatta
     7681 aagctagcaa tagacgttca attactttca ggctgattga gaattttaaa cctgcaagtt
     7741 ggaccttttt atatttgaaa acgagtatac aaatgattgc agcaattaat ggtgctagat
     7801 ctgtaacttc aaatataaag tgtttgacac caattataga ctggaaatct ctgagcataa
     7861 tggataacgc cattgtaatg acaaagaaga cgaaaatcgt cattgcccat tgaaaaccag
     7921 aaattttatt gttcttcatt atatgtaacc tccattaggt aacaagcaaa atatctttta
     7981 gtaacaatta tacatattac aagccgagag ataaattgtc tatgtcaata ttaatgaaaa
     8041 tgtaatagtc ttgacgtatt ttattaacat aaatcagcta agtattcgtt attacataga
     8101 aaataggggt aaaagtaatg tagataagta tttttaagat acggatttaa ctatgaattg
     8161 aattcaaaat ttcctatacg ttatacttta attgttaata agccacataa ataagagggg
     8221 gaaatgctgt gtacaagcaa ggtgaaccaa atttatggac tggaaggtta gatagtgaaa
     8281 cagacccgaa aaaatttaga cattttcaaa cagtaacatt tgaagatttg tctaagctgg
     8341 aaaagagtag tatgccatca ggggtcggta tattaggcta tgctgttgac aaaggtgttg
     8401 ctttaaacaa ggggcgcatt ggtgcaaaag aaggaccaga tgcgattaaa caagcatttg
     8461 caggtttgcc ggatttgaat caatgtgaaa ctttagtcga ttacggaaat gtttatcatg
     8521 atcatgagga attaattgat actcaaaaag aatttgctat gcttgcagcg aagtcaattg
     8581 ctaatcatag acaaacattt ttattaggtg gtggacatga tattgcgtat gctcaatatt
     8641 tagcaacacg taaagtctat ccaacacaat ctattggtgt gataaatatt gatgcgcatt
     8701 ttgatacacg tgctgaacaa caatctacat ctggaacgag ctttagacaa attttagaag
     8761 aagatgaaaa cacagattat ttagtgcttg gtattgctca aggtggtaat acgcaaagtt
     8821 tatttgatta tgctaaagag aaaaagattg attatgtctt tgcagatgaa ttattgagtc
     8881 acgtatcacc aacaattaaa gatatgattg aacgttttgt acatgaacat gatgtcatta
     8941 tgtttacgat ttgtatggat gtcattgata gtgcgtttgc gcctggggta agtgcgccgg
     9001 cagtgttagg tttgtatcca catactgttc ttgaattagc aaaacgtatt attccaagtg
     9061 ataaggtgtc ttcagttagt attgctgaga tgaatccaac atatgacgct gacaatagaa
     9121 ctgctaagct cgttgctaat ttagtgcatc attttttaaa ataattaaaa atatttatat
     9181 gtgacgagat ttgcattgca gggcagaatg tgagtatttc ttaatagaag ttatattaac
     9241 gttgatatat ttgaaaaata attgcagtaa gaagactctt aagttgtagt catcttactg
     9301 cttatttatg tttgttgaaa tagcaaaaat aacaactgta ttgtttgaaa agttctctta
     9361 ttatataata attaattaaa attttaacgg agttcaagag gcctttttat aatgacacct
     9421 tatttgtaaa atgtcatatg tggtttagcc tctttataat aatttaatct gttctcaagt
     9481 gtgccagtat gtaattctag cttatgacca tcagggtcgg taaagtaaat tgattgtcta
     9541 tctctaatat ctctaactct tccttctaaa atattcacgt tattatcttt taacctctga
     9601 tgccaatatt taaattcgct gtcatctata gtgaaagcta tatgtgtata tgaaaagtga
     9661 atttcattac gtggtatatc tttttcttca tttaaagcaa tccataggcc tgcaagctca
     9721 aaataagcag tttttttacc agtcaatagc aatttcccaa gtaaaatatc tctataaaaa
     9781 tgtattgaat cgtttaaatt tctgactgaa aagcatatat gattaataga ttttaacatc
     9841 tgcttcactt ctttcttaga atttttataa taaattattt ttgtatcgat ttttgaaatg
     9901 acgtcattaa tttttatacg atgatctgcg caactgcata agaggctcta ataacttaag
     9961 ttaaagagcc tctaaaagtc catataaata atcttggcat tgttgtacga atgtatgtac
    10021 ttgttcggag tgtttgcgtt ttttatggta attgatataa atttttcggc ctaagtttgg
    10081 acgaattttt ttatattcta aattagacgt gtgaaatgat tggtaataaa atctcggaat
    10141 gatagcgtaa cctaagccga gatgaacaaa tccaactgct gattcgaatc gatctgtttc
    10201 tacaacgaca tttggacgaa tatttcttct attaaaatag tcatctaagt gtttgcgcac
    10261 ttgagaattt ttgtttggta atatgagtgg caaattttca acatctaccc aattttgatt
    10321 tttaaatgtt tccttgggtg ctaataaaat gtaagattcc tcatataaag gaatggatct
    10381 tatatcttcg tgggttattt tttcatttgt tatagctaaa tgaatattaa aattcagtaa
    10441 ttgctctata gattgatgtt tatcatgtat ttcatataaa cgatattgct gttcagggta
    10501 gtcggaatgg tgctttcgaa ttaaattcgc aatccattga ttcgtagatt caagagtccc
    10561 aatttttatc cttggttctg atgtaacgct taaatcatac attttttcca tcgtggatcg
    10621 atattgttga actaattcgc tcgcataacg ataaaactgt attccttttt cggtaatctt
    10681 gatgtctttt gttgaacgtg taaataagtc ataacctaaa tctgcttcca tttttttaat
    10741 cgtagcagtt aaagatggct ggctaatatg taaaaattgt gcagctttag taaaactatt
    10801 atatttcacg atagccaaga agtattctaa ctgaataatt ttcatgtgtt ttgctccttt
    10861 tatttataga ttcaagctat ggataattag tttatttata tttgttggct atattttaac
    10921 atactataat gaatattgaa gataataatg taagcgtttt aaatttagtg gaattaactt
    10981 cttaaaagaa caggagcgaa ttttatgaga aaaattcaag caaaaaaagg tttgagtatt
    11041 gagtgtaaag gttgggaaca agaagcagta cttagaatgt tatacaacaa tttagaccca
    11101 gaagttgctg aaagaccaga agatttagtt gtttatggtg gtattggaaa agcagcacgt
    11161 aattgggaag cttttgaagc aatagagaaa acgttacgag aattagaatc agatgagaca
    11221 atgttagtac aatcaggaaa gccagttgct gtatttaaaa cacatgaaga agcgccacgt
    11281 gtattaattt caaattcagt attagtgcct gaatgggcaa actgggatca ttttaatgaa
    11341 ttagataaaa aaggtttaat catgtatgga cagatgacag ccggtagttg gatatatatt
    11401 ggttctcaag gtattgtgca aggtacttat gagacatttg ccgagctagg taatcaacac
    11461 tttaatggag atttagcagg cactgttaca ctgacagcag gtttaggtgg tatgggtggt
    11521 gcgcaaccgt tagcaattac tatgaatcat ggggtagcaa tttgcgtgga tgttgatgaa
    11581 acacgtgttg ataagcgaat tgatacgaaa tactgtgatg ttaaaacagc tgatttagat
    11641 gaagcattaa aattagcaga agaggcgaaa gaacgtggag aaggattatc aatcgggtta
    11701 gttggaaatg ctgtagacat ccatcaagcg attctagaaa aaggatttaa aattgacatt
    11761 attactgacc aaacaagtgc acatgatccg ctaaatggat atgtgccaca aggatactct
    11821 gtagaagaag cgaaagtatt gcgtgaaaaa gatccgaaaa aatatgttga actgtcacaa
    11881 gcttcaatgg caaagcatgt tgaattaatg cttgaattcc aaaaacgtgg cgctgtagca
    11941 tttgattatg gtaacaatat tcgtcaagta gccttcaata acggagtgaa aaatgctttt
    12001 gacttcccag gctttgtacc agcttacatt agaccattat tctgtgaagg taaagggcca
    12061 ttccgctttg ctgcgttgag tggtgatcca aaagatatcg agcgtgcgga tgaagaaatg
    12121 cgcaaacttt tcccagaaaa tgaaaagtta ttaagatggc ttgatttagc tgaagaaaaa
    12181 atttcatatc aaggactacc atcacgtatt gcttggttag gctatggaga aagagcgaaa
    12241 atgggcttag ctttaaatcg tcttgtacgt gacggtgaaa tttcagcacc aattgttatt
    12301 ggacgagacc atttagatgc tggttcagtt gctagtccta accgtgaaac agaaagtatg
    12361 aaagatggta gtgacgcggt tggtgattgg gctgtattga atgcactcat taacacagct
    12421 gcaggtggtt catggatttc attccatcac ggtggcggtg ttggcatggg atattcactt
    12481 catgcgggta tggttgttgt agcagatgga tcagagcgtg ctgaaagaag attggaacgt
    12541 gtattgacga ctgacccagg tatgggtgtt gcccgacatg ttgatgctgg ctatgacatc
    12601 gctattcaaa cagctaaaga aaaaggtatt catattccaa tgattgataa agcaggtgat
    12661 aagtaatgaa tgatttaata attaatcata tagcagaatt aattttaccg agatcaacag
    12721 ataaaccttt gaagggtaag gaattagatg aattaaatgt tgtgaaaaat ggtacagttg
    12781 tcattaaaga tggcaaaatt gtatatgctg gaacacatac agatgactac gatgcgactg
    12841 agacgattga tgctagtggg aaggtagtgt ccccagcatt agtagatgca catacccatt
    12901 taacatttgg tggatctcga gaacatgaga tgtcattaaa acgtcaaggt aagtcttacc
    12961 ttgaaatatt agaaatgggt ggtggcatat tatctaccgt taatgctact agagaaacat
    13021 ccgaagatga cttgtttaaa aaagcagagc atgatttgct tactatgatt aaacacggcg
    13081 tgcttgcagt tgaaagtaag agtggttatg gattagatag agaaaatgaa ctgaagcaat
    13141 tgaaagtgtc taatcgttta gctgagaaat atgatttaga catgaaacat actttcctag
    13201 ggcctcatgc tgtacctaaa gaggcaagtt caaatgaggc atttttagaa gaaatgattg
    13261 cgttacttcc ggaagtaaaa caatatgcag actttgcgga tattttctgt gaaacaggtg
    13321 tatttacaat agaacaatcg caacattata tgcaaaaagc caaagaagca ggttttaaag
    13381 tgaaaataca tgcggatgaa attgatccgt taggcggact ggaattagca attgatgaac
    13441 aagcaatatc agcagaccac ttagtagctt ctagtgataa aggaaaagaa aagttgcgaa
    13501 atagtgatac tgtagctgtc ctcttacctg caacgacgtt ctatttaggt aaagaagatt
    13561 atgcagatgc aagaggcatg cttgacaata acggtgcgat tgcattagca actgattata
    13621 accctggtag tagtgtcaca aacaacttac aacttgttat ggcgattgca gccttgaaat
    13681 taaagctatc acctaatgaa gtttggaatg ctgttacggt caatgctgct aaagcaatag
    13741 acattaacgc gggtacaatt aacacaggtg ataaggctaa tttagtaatt tgggatgcac
    13801 caaatcatga atatattcca tatcattttg gtatcaatca cgcagaaaaa gttattaaag
    13861 acggtaaggt catcgtagat aacacagtat cttttaaagc ataaaataat tagaaagctc
    13921 taagcattgg tttaaactaa tgtttagagc tttttgtggt ttgtaaaaac aagtatattc
    13981 gagaacacgt ttttgtatag agtctcaatt ataaaagtta gaatagtttg acaacagtgt
    14041 tacgatatta ttaaacaact gtctatcatt aacgaaagca attaacaaga gtacattgtt
    14101 gtgaaatcat cgtttaatag tcaaagatga tcatcgtagg gtagacaaca tatcattgac
    14161 ttaaggaagg ggaatgaccg atgggattaa aagatcaagc tgttacctgg agaaggtatt
    14221 ttcatcaatt tccagaacta tccgataaag aatttaagac tacacaaaaa attaaagata
    14281 ttttaacaga acatcatatt agaatattag acttaccact tgcgactggg cttgttgctg
    14341 aagtgggaca aggtctgagt tgtatagctg ttcgggctga cattgatgct ttaccgattc
    14401 aagagcttgt tgaacaggat tttaaatctg aaaatgaagg tgttatgcat gcgtgtggtc
    14461 atgatattca catggctagt attttggcta cagctgtaaa attaaaagaa attgagggca
    14521 ctcttactgg gcgtgttaaa ttcatttttc aatcagcaga agaactagga catggcgctt
    14581 ttaaaattat tgaaacacat gcattaaaag atgtacaagc agtattgggt tttcataatg
    14641 acccatcgcg ttcggtaggt acatttgcaa tcaaaacagg ggcaattaca tcagcagtag
    14701 atcgttttga gtttcatatt aaaggcgtgg gtggtcatgc tgcaaagcca gaacaatgca
    14761 acgatccagt tattgtgttg gcgcaattga ttaatagtat tcaatccata gttagtagaa
    14821 atctatctgc gtttgatgaa gcggtagtaa caattggaca aatatcatgt ggtaatactt
    14881 ggaatgtcat tgctgatcat gcgtatgtac aaggcacagt gcgtagtttt gatcctgtgg
    14941 tacgaaagct tgtagaaaca cgattacaag atattgcgga cggattggct caagcatata
    15001 atatgaaaat caatttaaat tatacgcatt tacctggcgc tgtgatgaat gatgaggcat
    15061 tgacgcataa agcaattgca gtagcccaac atgttggata taaggtggaa atgatggaac
    15121 agccgcttac gattggagaa gatttttcag gttatagtca acattttcca agtgtatttg
    15181 cgttaattgg ttctcatagt gaatatgatt tacatcatcc tcaatataaa ccggatgaaa
    15241 ggattctgga aaaagtacct gagtattttg ttgaatttgt aaagagacta ttgcatgaat
    15301 aattcgagct tgtttttata aagaagaaag taatttttag tgtaatcatg attaagttcc
    15361 taggaagatt gggtaaataa taaccattaa gcgaataagg agatgtaaaa aaatggcagc
    15421 tcaagatcct agaacaaaat ttaaaacaac ggattatgaa aaacaagaac aagaggtacc
    15481 gggtttacaa tctgaaatga cgccagcacc cgattgtggc gaaacgtctt accaaggtca
    15541 tcaacgctta caaggctata aaatattagt cacaggtggt gactcagcaa tagggcgtgc
    15601 agcagcaatt gcgtatgcta aagaaggtgc agatgtagcg attaactatc ttccgagtga
    15661 agaacaagat gcacaagaag tacgccaagt gattgaagaa agtggtcaaa aggcagtgtt
    15721 aattcctggt gatataagag atgaacagtt caactatgac cttgttgaac aagcatatca
    15781 acaattaggt ggcttagata atgttacttt agttgctgga catcaacagt atcatgatga
    15841 tattcatgga tttacaactg aggcatttac agaaacattt gaaacgaatg tctatccgct
    15901 attttggaca gttcaaaaag cattggaata tttaaaaccg ggtgcgtcaa ttacaacgac
    15961 atcatctgta caaggttata atcctagtcc tattcttcat gattatgccg cttcaaaagc
    16021 agcaattatt tcattaacta aaagtttttc agaagaatta ggaccaaaag gaattagagt
    16081 gaactgtgta gcgcctggtc cattctggtc accattacaa atttctggtg gacaacctca
    16141 aagtaaaatc ccaacatttg gtcaaaaaac acctttaggt cgtgcaggtc aacctgttga
    16201 gttatgtgga acttatgtgc tattagcgtc agaagaatca agttatacaa ccggacaagt
    16261 gttcggtgtt agcggtggag tgcaaataga ttaggtttgt atgaagcttt taattgaaca
    16321 acataatatt aatttagtta cttcaatcca ttaaattaaa atgaattggt gaagtgtatt
    16381 ttaaatagtc ggtaaaggtg tgtatcttta tcggcttatt ttaatactct gtcataagga
    16441 gcgtttgctc agggtgtgga ttataagttt taagcagaat aaactttctt aatgaggatg
    16501 tcaggacaac aacgaaaaag ttaaaaactg ttgcgggatt gggtttaggg acggttagtg
    16561 gaagaaagtt aaattcatta acaagtgaaa tgcctaaggc tagatattaa acgtcagaac
    16621 ggacaaagaa taataggata tgttttcaat taaatagcga agtatttata tgttaagatg
    16681 atgtaaatta catagaaacg ctacccgtag aagtactgat catagaaacg ctgataaacg
    16741 taaatttgca aactcgatat taaacaaagg ggttgttatt atggcattaa tagtatctag
    16801 agctatatta tttttgattt tagcattagc ttttggaata gcgactgttt ttcctgaaca
    16861 aaaattacca ttatatatag tattcacatt atttgtagca gttgcgatcg tttcgaatgt
    16921 atggtttaca catcaaaacg ttaaaaagca tatcattaag tttaacaaat acgtcgcata
    16981 ctttttatcc tttgtctcag ggttacttta cattggattt gtaatttata cattaactat
    17041 agggagctat gatgttttac caattattat atttgcaata agtatcgtat tggttcaaat
    17101 attagaaaat atactgggaa tcagtataaa aaatacgcgt ctatcaaaca aacagttgaa
    17161 agtcatctcc aagcaacgtg ttcagcaatt taaagtcata tcattaatat tagtaagtgt
    17221 gtctatcgtg ttcattttta ttgcattaat gcttaaatgg tggcttttac ttgttattag
    17281 cattattgtt ttgattatta ttacagtagc aatgattgtt tatgaaaaat attatacgac
    17341 tcctgattaa tttatgattg acgtactaag gattatcttg taacgtaaac gtaaataagc
    17401 atcacttatc tagagaggtg gagggactgg ccctatgaag cctcggcaac atctcgaatg
    17461 tgccaattcc agtaaccgta atggtttgaa gataagcagg taaagcacat gaaaacctct
    17521 ttcttcatcg tttgtgagaa agaggtattt ttaattggaa agcaggtaaa aaggatggaa
    17581 gtacataaaa agagcaatgc ttgggcatta ttccccttgt tattatttgt ggcgttgttt
    17641 ttaggcgtag gtattatcac aggtgatttt acttcaatgc cattaaatgt tgcaattacg
    17701 ataacggtaa ttgtggcgtt attaatgaat cgaaaagaat catttgctaa aaaagttgag
    17761 gtcttcacga aaggtgcagg ccattcaaac attgttttaa tgatgttgat ttttatttta
    17821 gcaggtgcat tttcaaatac agctgaaaaa atgggtggcg taaagtcgac agttaattta
    17881 ggactatcat taatcccaga aaatttaatt attgtaggat tgtttgtgat ttgtatgttt
    17941 gtttctatat ctatggggac gtctgtagga actgttgctg caatagcacc tgttggttac
    18001 ggatttgcac aagcgacaga tgtaccaact gcgctagcaa tggctacagt tgttggaggt
    18061 gcaatgttcg gcgataatct atcaatgatt tcagatacaa caattgctgc ggttagaaca
    18121 caacatacga aaatgaaaga taaattcaga gtaaacttta aaattgtatt gccaggtgct
    18181 attttaacga ttattattct gtatttctta acgaatggca tttctttaaa tcatgctaaa
    18241 aactatgatt ataatctaat taaagttgta ccgtatatct tagtattggt attagcatta
    18301 ttaggtgtaa atgtcattat cgtattgatt ggcggaacat tattagccgg aatcattggt
    18361 ctcatagatg gttcattcgg ttggatgggg ctactagatg cagtgtccaa aggtattata
    18421 agcatggaag atattgctat gattgcgttg ctaattggtg gtttagtcgg tattattcaa
    18481 cacaatggtg gtattgaatg gttgttgcaa tttgttcgct ctaaagtgaa atcaaagcgt
    18541 ggcgcagaat taggaattgc tagtttggta agtgtcgcag atattgccac ggcaaacaac
    18601 accatctcta ttattatgtc aggtccttta gctaaaaata ttgcagatga atatgatgta
    18661 gattcaagga aatcagcaag tattcttgat atatttggag gctgcttcca aggtttatta
    18721 ccatatagcc cgcaagtcat ttcagcggct ggtgtagctg gtatttcacc gttaatgtta
    18781 ttcccataca gcatttatcc tatattatta ggtgtatgcg gacttattgc gattttattt
    18841 aattttccaa agctaaaaaa gaacactagt caagaaatga aacattaatg cttaacaata
    18901 tatcgatgta gccaagtaag gtggcttgtt atattaacag atcatgttgt actcaccgta
    18961 taacgctaat cttttaaaaa tgatgatcat ctatgtttgg aactgagcaa aaatacgtta
    19021 aaataaaagt aagagcgaaa gtgggtggac aacagtggca aaatttaatg tagagaatga
    19081 acatgtcgaa gttgaaattg aaaagcttta taaattttct ccagagttag tatacgaagc
    19141 ttggacaaag aaagatttgt tgaaacaatg gtttatgaca tcagccagaa cgaataaaga
    19201 gattgaagcg gacgttaaag aaggcggtaa atatcgtatt gtagatcaac aacgtaatgg
    19261 caaagtaaat gtgattgaag gcatttatga atctttagtt atggatgaat acgttaagat
    19321 gactatcggt atgcctggac taagtgaaac gcaagatgtc attgaggtag aattttttga
    19381 acgtgaaaca ggtggcaccc aaatgctatt ttattatcgt tcattagttg aaaaagaaag
    19441 acgatttact aatttagaat acaagcaaaa gaaaaaagaa tatcatgatg ccatggtgca
    19501 tggtttcgaa ttgatgtttg ataagatgta ccacgttatc gaaacatcaa cacaacaata
    19561 aaatgataaa aggaagttat gataggctca aggccattaa atcataactt ccttttttct
    19621 aatttgaaat atcattat
//
LOCUS       DAKTGS010000038        19346 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.038, whole genome shotgun sequence.
ACCESSION   DAKTGS010000038 DAKTGS010000000
VERSION     DAKTGS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.038"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(523..1905)
                     /locus_tag="O0286_001152"
     CDS             complement(523..1905)
                     /locus_tag="O0286_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0169536.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
     gene            2009..2896
                     /gene="pdxS"
                     /locus_tag="O0286_001153"
     CDS             2009..2896
                     /gene="pdxS"
                     /locus_tag="O0286_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCY0169537.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            2900..3460
                     /gene="pdxT"
                     /locus_tag="O0286_001154"
     CDS             2900..3460
                     /gene="pdxT"
                     /locus_tag="O0286_001154"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCY0169538.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3668..4882)
                     /locus_tag="O0286_001155"
     CDS             complement(3668..4882)
                     /locus_tag="O0286_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0169539.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            5040..5501
                     /locus_tag="O0286_001156"
     CDS             5040..5501
                     /locus_tag="O0286_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCY0169540.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            5520..6086
                     /locus_tag="O0286_001157"
     CDS             5520..6086
                     /locus_tag="O0286_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCY0169541.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            6076..7083
                     /locus_tag="O0286_001158"
     CDS             6076..7083
                     /locus_tag="O0286_001158"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCY0169542.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            7097..9553
                     /locus_tag="O0286_001159"
     CDS             7097..9553
                     /locus_tag="O0286_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0169543.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            10038..11402
                     /gene="radA"
                     /locus_tag="O0286_001160"
     CDS             10038..11402
                     /gene="radA"
                     /locus_tag="O0286_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCY0169544.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            11427..12500
                     /locus_tag="O0286_001161"
     CDS             11427..12500
                     /locus_tag="O0286_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCY0169545.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            13057..14511
                     /locus_tag="O0286_001162"
     CDS             13057..14511
                     /locus_tag="O0286_001162"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCY0169546.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            14934..15581
                     /gene="cysE"
                     /locus_tag="O0286_001163"
     CDS             14934..15581
                     /gene="cysE"
                     /locus_tag="O0286_001163"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCY0169547.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            15565..16965
                     /locus_tag="O0286_001164"
     CDS             15565..16965
                     /locus_tag="O0286_001164"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCY0169548.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            16958..17362
                     /locus_tag="O0286_001165"
     CDS             16958..17362
                     /locus_tag="O0286_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCY0169549.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            17370..18116
                     /gene="rlmB"
                     /locus_tag="O0286_001166"
     CDS             17370..18116
                     /gene="rlmB"
                     /locus_tag="O0286_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCY0169550.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            18116..18640
                     /locus_tag="O0286_001167"
     CDS             18116..18640
                     /locus_tag="O0286_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCY0169551.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            18721..19290
                     /locus_tag="O0286_001168"
     CDS             18721..19290
                     /locus_tag="O0286_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCY0169552.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
BASE COUNT         7136 a         2645 c         3684 g         5881 t
ORIGIN      
        1 aaaatggatg cgatgagccg cattgagacc gcaaggtctc ttttttttat gtctaaaacg
       61 tcaaaataaa aagcaaacac aaagaaaaat ggcttggcga agtgaaaacg tttgaatctg
      121 acgaaacgag aaaagaacgc aacgagttta gtagagctaa atgagtaagt gagagccgaa
      181 ggagaggaaa gaagcaagcg attgtcacaa gtcaagaaag gttcttagcg aggatggtag
      241 ccaacttacg ttccgctaga gtagaactgg aaatgataat ttaataatgt acactttcga
      301 ttgtctaagt atgtacaact ttaattttgt gtttatataa atttaaaatg atatcatcga
      361 aaacaaaata ttgtataaat agagaagagc agtaagacgg tatctaattg aaaatgatct
      421 tactgctctt ttatatactt tattgaaata caaaaaggaa attaattatt atacaataga
      481 caagctattg cataagtaac actaactttt atcaaagaag tgttacttta taattaatga
      541 ttttattaga gcgtctacat gcggttttaa agcatcatcg tctataccgc caaagcctaa
      601 tataaattta ggggttttct tatagtcttg atcatcatca aaattataaa cttgtaattt
      661 taactttact ttgtttgctc tatcaagaca ctcttgtaat gttaatccat tttttactgt
      721 aattgtaaaa tgcatacccg tttcagcacc ttgaatatca agctgctctt tgtaaggttt
      781 caatcttttt aaaatatagg ttagttttct acgataaatt cgtctcattt tatttaaatg
      841 cctttcaaaa ccaccggaag atataaacgt tgcaataagg ttttgcatat gaacaggtac
      901 agtgttgcct tcaatgtgat tttgagaatg atattttttc attatagaat agggtaacac
      961 catatatgca actcgacagc taggaaaaat agactttgaa aatgtactga tataaatcac
     1021 tttttctcct cttgaatata gaccttgaat tgctggaatg ggtttgccga aatatctaaa
     1081 ctcggaatca taatcatctt ctataataaa tcgttcttct ttttcttgag cccattgtat
     1141 taattgagtt cgttttttta agtccatcac atatccagtt ggaaattgat gggaaggcgt
     1201 tatatatact atattttttt gtgatttaat aacttcatct acgtttattc cattatcttc
     1261 aacttcaatt tgttcatatt caacttgttt tttatctaaa atatttttga ttggtggata
     1321 actaggtttt tcgataataa atgttgaagt ataaagtaaa tcgactaatt gatttactaa
     1381 ttgttcggta gatgagccaa ttataatttg attaggatca caaattacgc cacgattagt
     1441 aaataaataa aatgccagtt gaaaccgcaa atgtaattct ccttgaaaat gtcctctacg
     1501 taattgattt aaatgatttg tatcataaag atctttggaa tactttctga aaagttctat
     1561 agggaaatgt ttcgtatcta tttcatccaa attaaaagca taatcataag cttcatcact
     1621 cgcttttggt ttatatgaat catcatcaaa aagagagggg ataggttgat tgtttaaaat
     1681 tgttaaagat tcaatttcgg acacaaaata tccagagcga ggtcttgaat aaatgtaacc
     1741 ttcgtctaat agaagttgat atgcatgctc tacggttgtt tggctaatag ataaatgttt
     1801 gcttaattgt cttttagaat aaaatttatc gccttcttta aattgacctt caattatttg
     1861 tttttttaat ttttcataaa gttgatggta taaagtgttt ttcaatttta taactgacct
     1921 cctaaattta tcttattttg taccttttta aatatcagtt tatacattac aatgtattta
     1981 atcaacttga aaaggggttt tatgtataat gagtaaaatt attggatcag acagagtcaa
     2041 aagaggtatg gctgaaatgc aaaaaggcgg cgttattatg gatgtcgtta atgctgagca
     2101 agcaagaatt gcagaagaag ctggcgcggt agcagttatg gcattagaac gagtaccttc
     2161 tgatattaga gctgctggtg gtgttgcacg tatggcaaac cctaaaattg tagaagaagt
     2221 aatgaatgct gtttctattc cagtcatggc taaagcacgt attggtcata tcactgaagc
     2281 aagagtatta gaggcgatgg gtgttgacta tattgatgaa tcagaagtgt taacaccagc
     2341 agatgaggaa tatcacttaa gaaaagatca atttacagta ccatttgtat gtggatgtcg
     2401 taatttaggt gaagctgcgc gtagaattgg tgaaggtgct gctatgttac gtactaaagg
     2461 tgaaccaggt acaggtaata ttgttgaagc tgtaagacat atgagacaag ttaattcaga
     2521 agttagtcga ttgactgtaa tgaatgatga tgagattatg acttttgcga aagatatcgg
     2581 tgcgccttat gaaattttaa aacaaattaa agacaatggt cgtttaccgg tagttaactt
     2641 tgcagctggt ggcgttgcga ctcctcaaga tgctgcttta atgatggaat taggtgctga
     2701 cggtgtattc gttggatcag gtatttttaa atcagaagat ccagaaaaat ttgctaaagc
     2761 aattgttcaa gcaacaacac attaccaaga ctatgaacta attggaagat tagcaagtga
     2821 acttggcact gctatgaaag gtttagatat caatcaatta tcattagaag aacgtatgca
     2881 agagcgtggt tggtaagata tgaaaatagg tgtattagca ttacaaggtg cagtacgtga
     2941 acatattaga catattgaat taagtggtca tgaaggtatt gcagttaaaa aagttgaaca
     3001 attagaagaa atcgagggct taatattacc tggtggcgag tctacaacgt tacgtcgatt
     3061 aatgaattta tatggattta aagaggcttt acaaaattca actttaccta tgtttggtac
     3121 atgcgcagga ttaatagttc tagcgcaaga tatagttggt gaagaaggat accttaacaa
     3181 gttgaatatt actgtacaac gaaactcatt cggtagacaa gttgacagct ttgaaacaga
     3241 attagatatt aaaggtatcg ctacagatat tgaaggtgtc tttataagag ccccacatat
     3301 tgaaaaagta ggtcaaggcg tagatatcct atgtaaggtt aatgagaaaa ttgtagctgt
     3361 tcagcaaggt aaatatttag gcgtatcatt ccatcctgaa ttaacagatg actatagagt
     3421 aactgattac tttattaatc atattgtaaa aaaagcatag cttaatgtat gctaaatcaa
     3481 cgaattattg atatttatag atttgttgag aagaaaatat ctccttcaaa cttagctttg
     3541 gaggagttat tttttatgtc aaaattaaaa atgataaaaa ataaagctat acataagaaa
     3601 aaaacccttc aaagagactg agaatagtca aaattttgaa ggggttaatt cgatgttgat
     3661 gtatttgtta aataaagaat ccagcgattg cagctgaaat gaaagatact agtgttgcac
     3721 cgaataataa tttcaaacca aagcgggcaa ctgtatctcc ttttttgtca ttaagtgatt
     3781 taatcgcacc tgaaataata ccgatagagc taaagttagc aaatgatact aagaatacag
     3841 atgtaacacc ttttgcgtgt tcagataaat cactaagttt accaagtgct tgcattgcta
     3901 caaattcgtt agataatagt tttgtcgcca taactgaacc ggcttgaact gcatcttgcc
     3961 atggcacacc gactaagaat gcaaatggtg caaagacaaa accaattaat gtttggaaat
     4021 cccaagaaat agcgccacct gaaactgtac taaagatatt gcttacaatt ccatttaata
     4081 gagcgataat ggcaatgtat ccgattaaca ttgcgcctac aatgacagct actttaaatc
     4141 catctaaaat atattctcct agcatttcga agaatgattg ttgtctttct tcagtttctt
     4201 caactaataa tttgtcatct tcttcattaa ctttataagg gttaataatt gaagcgatga
     4261 tgaaaccacc aaataagttt aagacaacag ccgttacaac atatttaggt tcaattaagg
     4321 taaagtatgc accgataatt gaagcagaaa cagtcgacat tgctgaagct gttaatgtgt
     4381 ataaacgttg cttaggtatg tatggtaatt gttttttaat tgaaataaat acttcagatt
     4441 gtcccaaaat tgctgcagca actgcattgt atgattctaa acgtcccata ccattaattt
     4501 tagaaattaa gaatcctaaa acattaatga ttaaaggtaa aatctttgtg tattgaagga
     4561 taccgataat cgctgaaata aatacgatag gtaataatac actgaagaag aatggtggtt
     4621 gcttaggatc gatatattga ataccaccga atacaaagtt aacaccatct gctgctttta
     4681 ataataagta gttaaaaccg tttgaaatac caccaataac cttgattccc attgtagttt
     4741 taagcaagat aaatgcaaag ataagctgaa ttgcaagtaa aattcctaca tatttccagc
     4801 gaatattttt cctgtctgag ctaaatagaa acgcaagtgc taaaaagaag ataattccga
     4861 taatcccaat tagaatatgc atatatttct cattccttta gttttttcta caatctatca
     4921 tacaataaaa tggaagggct aacatcataa atttttgaaa atataaaaac aaattaattg
     4981 aaaaaggtca aaataggtca tataatatag tcaaagaagg tcaaaaaggg gtgatataca
     5041 tgcacaatat gtctgacatc atagaacaat acatcaaacg tttatttgaa gagtcgaatg
     5101 aagatgtcgt tgaaattcag agagcgaata tcgcacagcg ttttgattgc gtaccatcac
     5161 aattaaatta tgtaatcaaa acacgattca ctaatgaaca tggttatgaa atcgaaagta
     5221 aacgtggtgg tggtggttac atccgaatca ctaaaattga aaataaagat gcaacaggtt
     5281 atattaatca tttgcttcag ctgattggac cttctatttc tcaacaacaa gcttattata
     5341 ttattgatgg gcttttagat aaaatgttaa taaatgaacg tgaagctaaa atgattcaag
     5401 cagttattga tagagaaacg ctatcaatgg atatggtttc tagagatatt attagagcaa
     5461 atattttaaa acgtttgtta ccagttataa attattacta aatgaaatga ggtgttgaag
     5521 tgctttgtga aaattgtcaa cttaatgaag cggaattaaa agttaaagtt acaagtaaaa
     5581 ataaaacaga agaaaaaatg gtgtgtcaaa cttgtgctga gggacaccat ccgtggaatc
     5641 aagctaatga acaacctgaa tatcaagaac atcaagataa tttcgaagaa gcatttgttg
     5701 ttaagcaaat tttacaacat ttagctacga aacatggaat taattttcaa gaagtagcgt
     5761 ttaaagaaga aaaacgttgc ccatcatgtc atatgacttt gaaagatatt gcacatgttg
     5821 gtaaatttgg gtgtgctaat tgttatgcaa catttaaaga tgacatcatt gatatcgtcc
     5881 gcagagttca aggtggacaa tttgagcacg ttggaaagac accacattct tcacataaaa
     5941 agatagcttt aaagcgaaaa atcgaagaaa agaatgaata tttgaaaaaa cttattgaaa
     6001 tccaagattt tgaggaagca gccattgtta gagatgaaat taaagcacta aaagctgaga
     6061 gtgaggtgca acatgatgac gcataatatt catgataata tcagccaatg gatgaaaagt
     6121 aatgaagaaa caccaattgt tatgtcttct agaattcggt tagcgcgtaa tttagaaaat
     6181 catgtgcatc cactaatgta tgctactgaa aatgatggat ttagagttat aaatgaggta
     6241 caagatgcct tgccaaactt tgaattaatg cgtcttgatc aaatggatca acaaagtaaa
     6301 atgaaaatgg ttgcaaagca tttgattagt cctgaactaa taaaacaacc agcagccgca
     6361 gtattagtga atgacgatga atctttaagt gtcatgataa atgaagagga ccatattcgt
     6421 attcaagcta tgggaactga cacgacatta caggctttat ataatcaagc ttcatcaatt
     6481 gatgatgaat tagatcgaag ccttgatata agttatgatg aacaacttgg ttatttaact
     6541 acatgtccta ccaatatagg tactggtatg agagcaagcg tgatgctaca tttgccaggt
     6601 ctatctatta tgaaaagaat gacacggatt gctcaaacca ttaatcgttt tggatataca
     6661 atcagaggta tttacggtga aggttcgcaa gtttatggac atacttatca agtatccaac
     6721 caacttacac ttggtaaatc tgagttagaa atcatagaaa cattaacaga agttgttaat
     6781 caaatcattc atgaagaaaa acaaatacga caaaagttag acacttataa tcaattagaa
     6841 acacaagacc gtgtttttcg ctcgctaggt attttacaaa actgtagaat gataactatg
     6901 gaagaggctt cttatagatt aagcgaagtt aaacttggta tagatttaaa ttacattgaa
     6961 ttacaaaact ttaaatttaa tgaattgatg gtagctatac agtcaccatt tttattagat
     7021 gaagaagatg acaaatctgt aaaagaaaaa cgagcagata tactaagaga acatataaag
     7081 taggaggtca ttatttatgt tatttggtag attaactgag cgtgcacagc gcgtattagc
     7141 acatgcccaa gaagaagcaa ttcgtttaaa tcattcaaat ataggaacag aacacctatt
     7201 attggggtta atgaaagaac ctgaaggaat tgctgcaaaa gtattagaaa gttttaatat
     7261 cactgaagat aaagtaattg aagaagttga aaaattaatc ggacatggtc aagatcatgt
     7321 tggtacattg cattatacac ctagagctaa aaaagtcatt gaattatcga tggatgaagc
     7381 tagaaaatta catcacaatt ttgttggaac ggaacatatt ttattaggct tgattcgtga
     7441 aaatgaaggt gttgcagcaa gagtttttgc aaatctagat ttaaatatta ctaaagcacg
     7501 tgcacaagtt gtgaaagctt taggaaaccc tgaaatgagt aataaaaatg cacaagctag
     7561 taagtcaaat aatactccaa ctttagatag tttagctcgt gacttaacag tcattgccaa
     7621 agacggtaca ttagatcctg ttataggacg tgataaagaa attacacgtg taattgaagt
     7681 attaagtaga cgtacgaaaa acaatcctgt gcttattgga gagccaggtg ttggtaaaac
     7741 tgctattgct gaaggtttag cgcaagccat agtgaataat gaggtaccag agacattaaa
     7801 agataagcgt gttatgtctt tagatatggg aacagtagtt gcaggtacta aatatcgtgg
     7861 tgaatttgaa gagcgtctga aaaaggttat ggaagaaatc caacaagcag gtaatgtcat
     7921 cctatttatt gatgagttgc atactttagt tggtgctggt ggtgctgaag gtgctatcga
     7981 tgcttcgaat attttgaagc cggcattagc acgtggtgaa ttacaatgta ttggtgctac
     8041 tacattagat gaatatcgca aaaatattga aaaagacgcg gctttagaac gtcgtttcca
     8101 acctgtacaa gttgatgaac cttcagtagt agatacagtt gctattttaa aaggattaag
     8161 agatcgttac gaagcacacc atcgtattaa tatttcagac gaagctattg aagcagctgt
     8221 taaattaagt aacagatacg tttcagatcg tttcttacca gataaagcaa ttgatttaat
     8281 tgatgaagca agttctaaag taagacttaa gagtcatacg acacctaata atttaaaaga
     8341 aattgaacaa gaaattgaaa aagttaaaaa tgaaaaagat gccgcagtac atgctcaaga
     8401 gtttgaaaat gctgctaacc tgcgtgataa acaaacaaaa cttgaaaagc aatatgaaga
     8461 agctaaaaat gaatggaaga atgcacaaaa tggcatgtca acttcattgt cagaagaaga
     8521 tattgctgaa gttattgcag gatggacagg tatcccatta actaaaatca atgaaacaga
     8581 atctgaaaaa cttcttagtc tagaagatac attacatgag agagttattg ggcaaaaaga
     8641 tgctgttaat tcaatcagta aagcggttag acgtgcccgt gcagggttaa aagatcctaa
     8701 acgaccaatt ggtagcttta tcttccttgg accaactggt gttggtaaaa ctgaattagc
     8761 tagagcttta gctgaatcaa tgtttggcga tgatgatgcg atgatccgtg tagacatgag
     8821 tgaatttatg gaaaaacacg cagtgagccg attagttggt gctcctccag gatatgttgg
     8881 tcatgatgat ggtggacaat taactgaaaa agttagacgt aaaccatatt ctgtaatttt
     8941 atttgatgaa attgaaaaag ctcatccaga tgtatttaat attctattac aagttttaga
     9001 tgatggacat ttgacagata caaaaggacg tacagttgat ttcagaaata caattatcat
     9061 aatgacatca aacgttgggg cacaagaatt acaagatcaa cgatttgctg gattcggtgg
     9121 ttcaagtgat ggacaagatt atgaaacaat tcgaaaaacg atgttaaaag aattaaaaaa
     9181 ttcattccgt ccagaatttt taaaccgtgt agatgatatc attgtattcc ataaactaac
     9241 aaaagaagaa ttaaaagaaa ttgtaacaat gatggttaat aaattaacaa atcgattatc
     9301 tgaacaaaac ataaatatta ttgtaactga taaagcgaaa gacaaaatcg cagaagaagg
     9361 atatgatcca gaatatggtg caagaccatt aattagagcg atacaaaaaa ctatcgaaga
     9421 taatttaagt gaattaatat tagatggtaa tcaaattgaa ggtaagaaag ttacagtaga
     9481 tcatgatggt aaagagttta aatatgacat tgctgaacaa acttcagaaa ctaaaacacc
     9541 atcgcaagca taattataaa acagtccaaa acaaattaaa gttttgggct gtttttttag
     9601 tagcattgaa ctatagaaat tcgtgaaagt atccatcaac gaaacaatct aataaaacaa
     9661 tcatcaaagg atagttaaga attatatgta acaagttaat gagcctacag cgatagcata
     9721 aaggtatgaa tttttataaa ggttttttgt ttgaagatac tagcaactta cgtcaaaata
     9781 aaataggtct atataatatt gtgataaatc tagagaaaag agtttactaa gaaattatta
     9841 agattttatc tttgaaagat accgactatc aacatgatgt aagtttattt tataatattc
     9901 ataaaaaata aatctggtaa aacagtttgc gttagtagtc atgttaaaat acaaacaaat
     9961 gtaactcaca tttaatttgt cataatggga atgtgcgttt aaaatagatt tgttcaaagg
    10021 aaagtggagg tgcaattttg gccaagaaaa aagtgatttt tgaatgtatg gcttgtggtt
    10081 atcaatctcc taaatggatg gggaaatgtc ctaattgtgg cgcttggaat caaatggagg
    10141 aaattgttga aaaagcagcc aatcctaaac atggagttaa aaccaaggaa ttagcaggta
    10201 aagtacaaaa attaaatagt attaaacatg aaacaacgcc gagagtgtta acagattcag
    10261 cagaattcaa ccgtgtatta ggtggaggta ttgtgagcgg atcgttagta cttattggtg
    10321 gggatccagg tattggtaag tcaacgttac ttttacaaat ttgtgcatcg ttatctcaaa
    10381 agaaaaaagt actatatatt actggagaag aatcgcttag tcagactaaa ttacgtgcag
    10441 agcgattaga tgaagattca agtgaattgc aagtattagc tgaaacagat cttgaagtta
    10501 tttatcaaac agtaaaagaa gaacaacctg atttattagt agtggattcg attcaaacaa
    10561 tatatcatcc tgaaatcagc tctgcgccag gttctgtttc acaagttcgt gaaagtacac
    10621 aaagtttaat gaatattgct aaacaaatga acattgcaac ttttatagtg ggtcatgtaa
    10681 cgaaagaagg tcaaattgct ggcccaagat tgctagaaca catggttgat actgtgcttt
    10741 attttgaagg cgatgaacac cacgcatatc gaattttgcg agctgttaaa aaccgttttg
    10801 gttcaacgaa tgaaatggga atcttcgaaa tgaagcaaag tggattaaaa ggtgtaaata
    10861 atccatctga aatgttttta gaagaacgtt caacaaatgt tccaggttca acaattgttg
    10921 caaccatgga gggaaccaga ccacttttaa tagaagttca agcgctggta actccaacga
    10981 cttttaacaa tccgagacga atggcaacag ggattgatca taatcgatta agtttgttga
    11041 tggctgtttt ggaaaagaaa gaaaattatc tattacaaca acaagatgct tatatcaaag
    11101 tagctggcgg tgtaaagtta acggagccag cagttgattt aagtgtaatt gtagcaactg
    11161 catctagctt taaagataaa gctgtcgacg gattagattg ctatattgga gaagttggtt
    11221 taacgggtga ggtacgtcgt gtatctcgga tagaacaacg cgtgcaagag gctgcaaaac
    11281 taggtttcaa acgtgtaatt attcctaaaa ataatatagg cggatggaca tatcctgaag
    11341 gtatacaagt aataggtgta actactgtac atgaagtatt gtcatttgct cttcattcat
    11401 aaaacatcaa gaaaggagga cattgtgtga atatcgttaa actaatggtt attattattt
    11461 acttaattat tgggagcgca ttaggaataa ttattattcc tgaaattgca aatgatcttg
    11521 gattacaaaa ctccagcttt ttaaaaaatc actatgtaga tggcattatc ggtagtattt
    11581 ttatgttctt aatttttggt gtatttatta gacgagttac taacgctata aaaggtttag
    11641 aacattttat tatgcgtaga agtgctgttg aaatactatt cgcaacaata ggtttaataa
    11701 tcggattact tatttctgtt atggtgtcgt ttatattaga atcaattggc aactctattt
    11761 ttaatcattt cattcctgtc ataattacga tattactatg ttatttcggt ttccaatttg
    11821 gccttaaaaa acgagatgaa atgttaatgt ttttacctga gaatatagcg cgttccatgt
    11881 cacaacatac taaaagtgct acgccaaaaa ttatcgacac aagcgcaatt attgatggtc
    11941 gtattttaga agtcattcgt tgcggtttta tcgatggcaa tattttaatt ccacaaggtg
    12001 ttattaatga attacaaatt gttgcagatt caaatgacag tgttaaacgt gaaaagggta
    12061 aaagaggctt agatatttta aatgaattgt atgatttaga ctatcctaca aaggttatac
    12121 atccaactaa aacacatagt gatattgata cgatgttatt aaaattagca aaacaatatc
    12181 atgcaagtat tataacgaca gatttcaacc taaataaagt ttgtcatgta catggtatca
    12241 aagcattaaa tgttaatgat ttatcagaag caatcaaacc taatgtacat caaggtgatc
    12301 aactgcatat tttactgaca aaaatgggta aggagcctgg tcaggcagta ggatatctag
    12361 atgatggtac gatggtagtt gttgataatg ctaaaaatct tattggcagt catgtcaatt
    12421 tagaagtagt cagcttattg caaacatctt caggaagaat tgtttttgct aaaaaaatcg
    12481 aagatacagt atcattataa aaactgtagt tgacgaaaac aaaatagata ttaaaaggta
    12541 tatgaaatgg cgaagtttgt aatttgaagt ataactataa ataaatgcaa aacatattag
    12601 ttatcaaaaa atacaaatag tgctaatata tcattgaaac aagttcatta agtgttaaat
    12661 tttagatatt aataaagtta ttcttatagc aatcaagaca tacaaaatta gaatcaattt
    12721 taacgtattt gaatagttaa tttgaagttt aatttcacag tacataatat tcatttttaa
    12781 gtcgttgaaa tgtttatgta gtgcagtctt gattcttagg gatagctttt ttaaagtgtt
    12841 gaaaaaacaa cagctttcta tttaaaattt aagcatatta cccagggaat tatgttatga
    12901 ttaaacaatg aaattatttt aaggtcatgt taaaaagcat tgtttcgttt taacaataag
    12961 acgagttaga catttgttat gacggtcgaa gatagcaaat gaacaattta tatgaactaa
    13021 cttgaaaatc tgatatttaa tttaggagtg aaaaaaatga gcgatcgtat aagagtaaga
    13081 tatgcaccaa gtccaactgg gtatcttcat attggtaatg caagaacagc attattcaat
    13141 tacttgtatg ctaaacatta caacggagat tttgtgattc gaattgaaga tactgataaa
    13201 aaacgtaatt tagaagatgg agaaacatca caatttgata atcttaaatg gttaggatta
    13261 gattgggatg agtctgtaga taaagacaat ggctacggac catatcgtca atctgaacgt
    13321 caacatatct accaaccatt aatagatcag ttactagcag aagataaagc atataaatgc
    13381 tatatgacag aagaagaatt agaagctgaa cgtgaagcgc aaatcgctcg tggtgaaatg
    13441 cctcgctatg gtggtcaaca tgcgcatttg actgaagaac aacgtcaaca atttgaagca
    13501 gaaggacgcc aaccatcaat tcgtttccga gtacctcaaa accaaacgta ttcatttgat
    13561 gatatggtaa aaggaaatat ttcatttgat tcaaatggta ttggtgactg ggttatcgta
    13621 aaaaaagatg gcattccaac gtacaatttt gcagtagcta tagatgatca ttacatgcaa
    13681 atttcagatg taattcgtgg tgatgatcat atttcaaaca cgcctaaaca aattatgatt
    13741 tatgaagcat ttggctggga gccacctcgt tttggtcata tgtcattaat tgttaatgaa
    13801 gaacgtaaaa agttaagtaa acgtgatggg caaattttac aatttattga gcaatatcgt
    13861 gacttaggtt atttacctga agcgttattt aattttattg cgttattagg ttggtctcct
    13921 gaaggtgaag aagaaatctt ttctaaagaa gaatttatca aaatctttga tgaaaagcgt
    13981 ttgtcaaaat caccagcatt tttcgataag caaaaattag catgggttaa taaccaatat
    14041 atgaaacaaa aagatactga aacagtattc caattagcat tacctcattt aattaaagca
    14101 aatttgattc ctgaggtgcc gtcagaagag gatttatctt ggggacgcaa attaattgcg
    14161 ctttatcaaa aagaaatgag ttatgccggt gaaattgtac ctttatcaga aatgttcttt
    14221 aaagaaatgc cagctcttgg tgaagaagaa caacaagtga ttaatggaga gcaagtacca
    14281 gagttaatga cgcacttatt cagtaaatta gaagcacttg aaccatttga agcggctgaa
    14341 attaaaaaga caattaaaga agttcaaaaa gaaacaggaa taaaaggcaa gcaattattt
    14401 atgcctattc gtgttgctgt aacaggccaa atgcatggtc ctgaattacc aaatacaatt
    14461 gaagtacttg gtaaagaaaa agtgctaaac cgtttaaaac aatataagta atgaaataga
    14521 caagatcaag gtagtatata cttgaatttt ataacgtaac cacatatgat aaagttgtat
    14581 taacaaatta caacaatatg aataaagtat tttatataaa acgaaggatt agtaattaaa
    14641 tttatacgat gcagagagtg tacggttgct gtgagtacaa cgtagaaatt aatgaatgca
    14701 ccttcgtaaa atgaattaaa tatataatga gagtgatgag cattaagttg acttagtttc
    14761 cttgataatt tggaagcgcc cgcaatatta ttaatgttat tcgctaaatt cagagtggaa
    14821 ccgtgcggaa gcgcctctaa caatacaact tgtatgttag tggtgctttt ttgatattta
    14881 atttcgcagg tacttcaatt atgtatttac gtagttcaat cattgaggag gaaatgatct
    14941 tgttaaaaag aatgagagac gatataaaaa tggtatttga gcaggatcca gcggcacgtt
    15001 caacattaga agtcattaca acgtatgcag gtttacatgc agtttggagt catttgattg
    15061 cacataagtt atacaaccaa aaaaaatatg ttgcagcacg cgcgatatct caaatttcaa
    15121 gatttttcac aggtatagaa atccatccag gtgctaaaat tggaaagcgt ctatttatag
    15181 atcatggtat gggcgttgta ataggagaaa catgtacaat tggtgataat gtgacaatct
    15241 atcaaggcgt gacacttggt gggacaggga aagaaagagg gaaaagacac ccagatatag
    15301 gagacaatgt tttaatagca gccggtgcga aagttttagg aaatattaaa ataaattcaa
    15361 atgtaaatat tggtgcaaat tcagttgttt tacaatcagt tccaagttat tcaacggttg
    15421 ttggtatacc aggacatatt gttaagcaag atggtgttcg agttggaaaa acatttgatc
    15481 atcgccatct acctgatcca atttatgaac aaattaagca tttagaacga caacttgaaa
    15541 agactaggaa tggagagatt caagatgatt acattatata atacgcttac acgtcaaaaa
    15601 gaagtgttca agcctataga accagggaaa gtaaaaatgt atgtatgtgg tcctactgta
    15661 tataactaca ttcatattgg taacgcaaga ccagcaatta attatgacgt agtgagacgt
    15721 tactttgaat accaaggata taatgtagaa tatgtatcaa attttacaga cgtagatgat
    15781 aaattaatta aacgttctca agaattaaat cagtctgttc ccgaaattgc agaaaaatat
    15841 atcgctgctt ttcatgaaga tgttggtgcg ttaaatgtta gaaaagcgac ttcaaatcca
    15901 agggtaatgg accatatgga tgacattatt caatttatta aagatttggt ggatcaaggt
    15961 tatgcatatg aaagtggtgg cgatgtttac tttagaacac gtaaatttga aggttatggt
    16021 aaattaagtc atcaatccat agatgactta aaagtgggtg ctcgtataga tgcaggagag
    16081 cataaagaag atgcacttga ttttacattg tggaaaaaag cgaagcctgg cgagattagt
    16141 tgggatagcc catttggtga aggtagacca ggatggcata tagaatgttc tgtaatggca
    16201 tttcatgagc taggacctac aattgatata catgcgggtg gttcagattt acaatttcca
    16261 catcatgaaa atgaaatagc acaatcagaa gcacataatc atgcgccatt tgctaattat
    16321 tggatgcata atggtttcat taatattgat aatgaaaaaa tgagtaaatc actaggcaac
    16381 tttattttag ttcacgatat tattaaagaa gttgatccag atgtactaag attctttatg
    16441 attagcgtac attatagaag cccaattaac tataatctag aattggtaga atcagcacgt
    16501 agtggactag agcgtattcg caatagttat caattaattg aagagcgcgc acaaattgct
    16561 actaatattg aaaatcaaca gacatatatt gatcaaattg atgcgatttt aaatcgtttt
    16621 gaaacagtta tgaatgatga ttttaataca gctaatgcaa ttacagcttg gtatgattta
    16681 gcaaaacttg cgaataaata tgtactagag aacacaacat caacagaagt aattgataaa
    16741 tttaaagcag tttatcaaat tttcagcgat gttttaggtg taccgttaaa atctaaaaat
    16801 gcagatgaat tattggatga agatgttgaa aaattaatcg aagagcgtaa tgaagcaagg
    16861 aaaaacaaag attttgcacg agcagatgaa attcgagaca tgctgaaatc acaaaacatt
    16921 atattagaag acacacctca aggggttaga tttaaacgtg gataatcaac aagataatca
    16981 cattaaatta ttgaatccat tgaccttagc atatatggga gacgcagtct tagatcaata
    17041 tgtacgtacc tatatcgttt taaagcttaa aagtaagcct aataaactac atcaaatgtc
    17101 taaaaaatat gtatctgcca aaagtcaggc gcaaacgtta gaatatttaa tggagcaaga
    17161 atggtttaca gacgaagaaa tggatatttt gaagcgaggg cgtaacgcga aaagtcatac
    17221 taaagctaaa aacactgatg ttcaaacata tcgtaaaagt tcagcgatag aagcagtgat
    17281 aggttttctt tatttagaaa aaagagaaga acgattagag gcattattaa ataaaataat
    17341 aacaatagta aacgaaaggt agtgacgatg tggaagatac ggttattgtt ggtaggcatg
    17401 ctgttagaga agcgattatt actgggcatc cgataaataa gatattgatt caagaaggta
    17461 ttaaaaagca acaaattaat gaaattttaa aaaatgcaaa agatcaaaaa atcattgttc
    17521 aaactgtacc aaaatctaaa ttagattttt tagcaaatgc accacatcag ggtgttgcag
    17581 cgcttattgc accatatgaa tatgctgact tcgatcaatt tttaaaacag caaaaagaaa
    17641 aagaaggttt attgacagta cttatattag acggcttaga agacccacat aacttgggat
    17701 caattttaag aacagccgat gcaacgggag ttgatggtgt tattattcct aaacgtcgtt
    17761 cagttacact aacgcaaaca gttgcaaaag cctcaacagg tgcaattgaa catgtaccag
    17821 ttattcgagt gacaaattta gctaaaacta tcgatgaact aaaagataat ggcttttggg
    17881 tagctggcac tgaagctaat aatgcaacag attatagaaa tctagaagcg gacatgtcat
    17941 tggctattgt aattggtagc gaaggacagg gtatgagtcg cctagtaagt gataaatgcg
    18001 atttttatat taagattcca atggttggac atgtaaacag tttgaatgct tcggttgcag
    18061 caagtttaat gatgtacgaa gtatttcgaa aaagacatga tgttggagaa atataatgaa
    18121 agaacgttac ttaatcattg atggatacaa tatgatagga caatcaccaa cgctaagcgc
    18181 cattgcaaaa gagaatttag aagaagctag aatgcaatta atagatgcaa ttgcaaatta
    18241 taatgcagtt atttcagatg aaattatttg tgttttcgat gcttatgacc aatcgggtgt
    18301 tgaaagagaa tacatgtatc atggcgttaa aacgattttt accaaggaaa aagaaacagc
    18361 tgatagtttc atagaacgtt atgtttatga actttatgac aagcatacta agcatattac
    18421 agttgtaaca agtgatatga gtgagcaaca tgctatcttt ggatcaggtg catatagaat
    18481 atcatctcgc gaaatgtgga gagatttaaa agaaaatgaa attgatgtga gtaaatcatt
    18541 agatgatata agtgaaaaca agccaagaac tcgaattccg ttatcttctg aaatccttgc
    18601 agaatttgaa aaaatacgaa gaggacatca taagaaatga catttccgtc atcttgaaat
    18661 tttgaatgta acaatattaa actaacctta aattttagat agaaggggtt agtttaatat
    18721 ttgaaatacg atttgacaac tcaagacagt acaatcaaac gtaacaatgc aataaatgat
    18781 aaagacttcg aaaagttagt aatggatcta caaccattaa ttattcgacg catcaaaaca
    18841 tttggattta atcattatga tttagaagac ttatatcaag aaatacttat acggatgtat
    18901 aggtcggtcc aaacatttga ttttagtgga gagcagcctt tcacaaatta tgttcaatgt
    18961 ttaattacgt ctgtaaagta tgattatttg agaaaatatt tagctacaaa taaaagaatg
    19021 gataatttga ttaatgaata tagagttacg tatccatgtg caataaagcg ttttgatgtt
    19081 gaaaacaatt atttgaataa attagcaatt aaagagttga tttgtcagtt taagtatttg
    19141 agtgcatttg aaaaagatgt catgtattta atgtgtgaac aatataagcc gagagaaatt
    19201 gctcaattga tgcatgtaaa agagaaagtg atttataatg ccatacaacg atgtaaaaat
    19261 aaaataaaac gttatttcaa aatgatttga aaagcgcctt aggacgtgaa ttgaattata
    19321 acgtgttact tactgatggt ttgaca
//
LOCUS       DAKTGS010000039        19263 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.039, whole genome shotgun sequence.
ACCESSION   DAKTGS010000039 DAKTGS010000000
VERSION     DAKTGS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.039"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(142..981)
                     /locus_tag="O0286_001169"
     CDS             complement(142..981)
                     /locus_tag="O0286_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0169553.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(1129..1236)
                     /locus_tag="O0286_001170"
     CDS             complement(1129..1236)
                     /locus_tag="O0286_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169554.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            complement(1390..2500)
                     /gene="prfB"
                     /locus_tag="O0286_001171"
     CDS             complement(join(1390..2427,2429..2500))
                     /gene="prfB"
                     /locus_tag="O0286_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCY0169555.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(2669..2773)
                     /locus_tag="O0286_001172"
     CDS             complement(2669..2773)
                     /locus_tag="O0286_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169556.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            complement(2815..5346)
                     /gene="secA"
                     /locus_tag="O0286_001173"
     CDS             complement(2815..5346)
                     /gene="secA"
                     /locus_tag="O0286_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCY0169557.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            complement(5760..6332)
                     /gene="raiA"
                     /locus_tag="O0286_001174"
     CDS             complement(5760..6332)
                     /gene="raiA"
                     /locus_tag="O0286_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCY0169558.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(6393..7067)
                     /locus_tag="O0286_001175"
     CDS             complement(6393..7067)
                     /locus_tag="O0286_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCY0169559.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(7060..8142)
                     /locus_tag="O0286_001176"
     CDS             complement(7060..8142)
                     /locus_tag="O0286_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0169560.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(8506..9372)
                     /gene="fakB1"
                     /locus_tag="O0286_001177"
     CDS             complement(8506..9372)
                     /gene="fakB1"
                     /locus_tag="O0286_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCY0169561.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            9516..10157
                     /locus_tag="O0286_001178"
     CDS             9516..10157
                     /locus_tag="O0286_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCY0169562.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(10327..11382)
                     /locus_tag="O0286_001179"
     CDS             complement(10327..11382)
                     /locus_tag="O0286_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCY0169563.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            11700..12770
                     /locus_tag="O0286_001180"
     CDS             11700..12770
                     /locus_tag="O0286_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCY0169564.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            12966..13727
                     /locus_tag="O0286_001181"
     CDS             12966..13727
                     /locus_tag="O0286_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0169565.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            13745..14239
                     /locus_tag="O0286_001182"
     CDS             13745..14239
                     /locus_tag="O0286_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0169566.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            14253..15479
                     /gene="pepT"
                     /locus_tag="O0286_001183"
     CDS             14253..15479
                     /gene="pepT"
                     /locus_tag="O0286_001183"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCY0169567.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(15668..16792)
                     /locus_tag="O0286_001184"
     CDS             complement(15668..16792)
                     /locus_tag="O0286_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0169568.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(17217..17537)
                     /gene="ytxJ"
                     /locus_tag="O0286_001185"
     CDS             complement(17217..17537)
                     /gene="ytxJ"
                     /locus_tag="O0286_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCY0169569.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(17691..18569)
                     /locus_tag="O0286_001186"
     CDS             complement(17691..18569)
                     /locus_tag="O0286_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCY0169570.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            18689..19207
                     /locus_tag="O0286_001187"
     CDS             18689..19207
                     /locus_tag="O0286_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCY0169571.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
BASE COUNT         6170 a         3423 c         2731 g         6939 t
ORIGIN      
        1 ttatactttc tttttacata tcaatcaatt aaatatcatt taaatatctt ctttatataa
       61 ctctgattaa atgataccaa aaaatcctct caacctgtta cttaaacagg ctaagaggat
      121 agtcttgtct tgatatatta cttagtggat gtaattatat tttcctggat ttaaaattgt
      181 tcttgaagat ttaacattaa atccagcata gttcatttca gaaacagtaa ttgttccatt
      241 agggtttaca gattcaacaa caccaacatg tccatatgga ccagcagctg tttggaaaat
      301 agcgccaact tctggtgttt tatctacttt aaatcctgca acttttgctg cgtaattcca
      361 gttatttgca ttgccccata aacttcctat acttctacct aattgtgcac gacgatcgaa
      421 agcataatat gtgcagtttc cataagcata taagtttcct ctgttagcaa ctgatttatt
      481 gtagttatgt gcaacaggta cagttggtac tgatttttgt acttgagcag gtttgtatgc
      541 tacattaact gtcttagtta ctgcttgctt aggtgcttgc ttaactacta cttttttaga
      601 tgcttgttgt acaggttgtt ttactacctt tttagcttgg cttgcttttc ttactggtga
      661 tttaaccgct ttagtttgtt tcactttatt ttgaggcaca agtgaaatca cgtcaccagg
      721 aaaaattaaa ggtgttacac caggattgta tgaatataat tgattcaacg ttaagtgatg
      781 ctctaaagca atcttatata atgaatcgcc agcaactact gtataagttg tcggtgattg
      841 cgtttgtgct tgaacatttg atacataatt atgttgaaca ggtgttttta cttgtgtgcc
      901 atgttgttgt gcatgtgctg cattatttaa agctaaaaaa gctaacactg acgaaaccgt
      961 cactgtaaga gattttttca tcttgctgtc attcctttgc tgttagtatt ttaagtatgc
     1021 aaatactata gcacaataca ttttgtcaaa agctattgtt ataacgatgt aatcaaatgg
     1081 ttaacaatat aaaaagaata caacctttta tcatagtgta aaatgtattc ataccatgta
     1141 attgagaacg ttttcaataa ttaattcaat accttgaaaa tcgccatagg taatattact
     1201 aaatgcacac tgcatatgtt gttttaacaa acacaacttt taaaaaatat attctaactc
     1261 tatctaccga attgtactta aatattcata aacaaatcat attccaaaat ctaatttaca
     1321 atttatttag ctacctttaa aaaaaccaaa aaccgacgcc cttttagagc ctcggtttta
     1381 aaatatatat taatcgtgcg acattgtctg tcttaaatat gattcgataa atggtccaat
     1441 gtctccatcc atcactgcat caaccttacc tgtttcttcg ttcgtacgat gatctttcac
     1501 cattgagtat ggatggaaaa catatgatct aatttggctt ccccagccga tttctttttg
     1561 ttcgccacga atttcagcca tttcacgtgc ctgctcttcc aattttaatt gatataattt
     1621 agactttaac attttcatag ctgcttcacg gtttttaatt tgagaacgtt cattttggtt
     1681 attaacaact atacctgagg ggtggtgggt aattcgtatt gccgattcag ttttgttaat
     1741 atgctgacca cctgcaccag aagctctgaa tgtatcaact gtaatatcat ccggattgat
     1801 ttcaatctct atttcatcat tattaaaatc tggaataacg tcgcatgatg caaatgatgt
     1861 atgacgacgt cctgatgaat caaatggaga aattcgtact agtcggtgta cacctttttc
     1921 agcttttaaa taaccataag cattatgccc tttgatgagc aatgttacac ttttaatccc
     1981 cgcttcatcc ccaggtagat aatcaacagt ttcaacttta aagcctttct tctcacaata
     2041 acgttgatac attctaaata gcatattagc ccaatcttga gactccgtgc cacctgcacc
     2101 aggatgtaac tctagaattg cgttattggc atcgtgaggc ccatctaata ataattgcaa
     2161 ttcgtattca tccactttag ccttaaaatt aatgacctct tgctctaagt cttctttcat
     2221 ttcttcatca aattcttctt gtaataaatc ccaagtagca tccatgtcat ctacttctgc
     2281 ttgtagtgtt ttataaccat taactattgc ttttaacgca ttatttttat ctataatatc
     2341 ttgcgctttc gtttggttat cccaaaaatt aggttctgcc atcatttctt catattcttg
     2401 aatattagtt tctttgttct ctaagtcaaa gagaccccct aatttgtgtt aaatcttgat
     2461 tatacttatc tatatttcgt ttgatttctg ataattccat agcattcgct cctatttata
     2521 tttcaattca agtcattgat ttgcatcttt tataatgcta aattttaaca taattttgtt
     2581 aaataacaat gttaagaaat ataagcacac tgacaattag tttatgcatt tattgttaaa
     2641 aatcagtaca tttatcatcg acatatgcct aaaccgattt tttaaaacta agtacataac
     2701 aacgtttaac aacttcttca cattttttaa agtatttaac gcttgtaaaa taaaaagact
     2761 cctcccataa cacaaactat aggtgtttaa ttggaaggag ttattttata tcatttattt
     2821 tccatggcaa tttttgaatt ttttaccact accacatgga caatcatcgt tacgaccaac
     2881 ttgatcgcct ttaacgattg gtttcggttt cactttttct ttaccatctt cagctgaaac
     2941 gtgcttcgct tcaccaaact ctgttgtttt ttcacgttca atattatctt caacttgtac
     3001 tacagatttt aaaatgaatt tacaagtatc ttcttcaata ttttgcatca tgatatcaaa
     3061 taattcatga ccttcatttt gatagtcacg taatggattt tgttgtgcat aagaacgtaa
     3121 gtgaatacct tgacgtaatt gatccattgt gtcgatatga tcagtccaat ggctatcaat
     3181 agaacgaagt aaaatcatac gctcaaactc attcatttgt tcttctaaga tatctttttg
     3241 actttgatat gctgcttcaa tcttagccca aacgacttcg aaaatatctt cagcatcttt
     3301 acctttgata tcatcctctg taatgtcacc ttcttgtaag aagatgtcat taatgtagtc
     3361 gatgaatggt tgatattcag gctcgtcatc tgctgtatta atatagtaat tgatactacg
     3421 ttgtaacgtt gaacgtagca ttgcatctac aacttgagag ctgtcttctt catcaataat
     3481 actatttctt tcgttataga taatttcacg ttgtttacgt aatacttcat cgtattctaa
     3541 gatacgttta cgcgcgtcga agttattacc ttctacacgt ttttgtgctg attctacagc
     3601 tcttgatacc atttttgatt caattggtgt agagtcatct aaacctagtc ggctcatcat
     3661 tttctgtaaa cgttcagaac caaaacgaat cattaattca tcttgtaatg ataaatagaa
     3721 gcgactatcc cctttatcac cttgacgtcc agaacgacca cgtaactggt catcaatacg
     3781 acgagattca tgtcgctctg tacctattac tgctaaaccg cctaattcct ctacgccttc
     3841 acctaatttg atatctgtac cacgaccagc catgttagtg gcaatagtaa cggcaccttt
     3901 ttgtccagcg cctgcaacaa tttcagcttc acgttcatga tttttcgcat ttaacacatc
     3961 atgacggata ccacgttttt taagtaaatt tgaaatatat tcagaagtct caactgcaac
     4021 agtacctaat agcactggtt gccctgcctt gtgtttttca acaacatctt ctactactgc
     4081 atcaaattta cctttttggc taatgtaaat taaatcagac ttatcgttac gttgcacagg
     4141 tttatttgtc ggaatttgag ttactgtcat gttataaata tttctaaatt cttcttcttc
     4201 agttttagct gtacctgtca tacccgcaag tttattgtac attctgaaat agttttggaa
     4261 tgtaatagac gccatagttt tagattcatt ttgaatttga acgccttcct tcgcttcaat
     4321 agcttggtgt aaaccttccg agaaacgacg gcctggcatt gtacgtcctg taaattgatc
     4381 gacaattaat acttcgccat caacaaccat atagtctacg tcacgttgta atgtaacgtg
     4441 cgcacgtaaa gctgtgttga tatgactaat aacatcaaca ttttgtacat catataagtt
     4501 ttcaactttg aacatacgtt cagctttatc cgcaccttgt tctgttaaat gtacagcttt
     4561 cgttttttca tcgtatttat aatcttcgtc ctgttttaac attttcgcaa aaacatttgc
     4621 ttgtgtataa agtgacgttg acttttcagc ttcaccagaa ataattaatg gcgtacgtgc
     4681 ctcgtcgatt aaaattgagt caacctcatc aatgattgca aaatgtaatg gacgcattac
     4741 tctatcttca gaataattca ccatgttatc tcgtaagtaa tcaaaaccta gctcattatt
     4801 agtactgtaa gtaatgtctt gtgcgtatgc ttcacgtttt tcttctgtcg tcttactgtt
     4861 taagtttaat ccgacagtca aacctaagaa gttatataac tcagccattt cttcactttg
     4921 aacacttgat aagtattcat tgactgtaat aacgtgaaca cctctaccag ctaatgcatt
     4981 taagtatgtt ggcattgtcg ctgttaatgt tttaccttca cctgttctca tctcagcgat
     5041 atcaccttta tgaattgcaa taccacccat aatttgaact ttatatggtg tcatattgaa
     5101 tacacgttta gagccttctc taacaagtgc atatgcttct ggtaaaattt tatctaaata
     5161 atcattttgc tttttgacat tatcaatgtc agctaattct gtttggaatt gtttcgtttt
     5221 attacgaatt tcttcatcag ttaaaattgc cgttttttct tctaaagcga ttactttatc
     5281 agcaagttta cctaactgtt taatttcttt attattgcca tcaagaattt ttgataaaaa
     5341 tcccatttcg ttcgctcctt tagctaaaaa actgtttggc ctacaacaat atatcttatc
     5401 atttatagtt agaaaattat acttatttac tcatttgtag aatcaatata aatatattta
     5461 tgacatactt cattcacatt ctgttgtcaa caagtttatc actaataaat atattctcaa
     5521 tacgcaatta tacttcctaa taaattatat tataaatatt ttacgatttt cgactcggac
     5581 tatacaatag actgacatac tattattaac ttaacattca aatatataca tccattaaca
     5641 ttagcatagt cactatgttt cattcaacaa attacattat cgaactatga aatagtcata
     5701 atttgctttt ggagtataaa aaagcacttg tgcaaaaaca caagtgcttt aaacttaatt
     5761 tattgttcac tagtttgaat caagccatat ttaccgtctt tacggcggta aacgatactt
     5821 gttccatcag tttctctgtc tgtgaataca aagaagtcat gacctaatag attcatttgt
     5881 aataccgctt cttctgaatc cattggtttt aagctgaatt cttttgaacg aataatttct
     5941 atctcgttat catcgtaagc gtcattatca acttgtgttt cttgcatttc ttgtaattcg
     6001 gcaacaaaca cttcttgatc tcctcgatca cggctcttac gattaatacg tgttttatat
     6061 tttcgaactt gtctttcaag tttattatta attaaatcaa tacctgcgta taaatcatcg
     6121 tttcgctctt cagctcttaa cgtaacattt ttcaatggaa ttgttacttc aattttagta
     6181 gctgaatttg aataagtttt aactttaaca tgcgccactg catttggtac gtcattaaaa
     6241 taacgttcca acttaccaat tttttcctca atatagttgc gaatagcatc tgtgatagtg
     6301 aggttatctc catgaatttc aaatctaatc atagtaatct ctccttaaac ctctttattg
     6361 gtaactcttt attatatttt aacattttta cgctatcgtg caaacgcaaa cactttgaat
     6421 tttctgatat ttttagcata taatttacac cctgcacgat gaattgttaa tccagttgta
     6481 taaatatcgt caacgagtaa tatttcctta ccatttaaat ctaattccgt atcaataata
     6541 aatggatttt catctgccag acgctctttc ttagttaaat gagactgttt tggtctattt
     6601 gacatcttta aaatcttatc aaagcgaatc cctttagctt ttagtactgc ttctaccggg
     6661 ttaaatgttc tagataaatc atgtgccggc gaagaaggaa ttggcacaat atagtcataa
     6721 gatgtttgtg gtatttcaat caaatgtgcc aataattcac ataaataata gtctttcaaa
     6781 aatttatact gatgtatcat ctctttcatt aaaccgtcat attgaaattg acaatataat
     6841 tgttccatta aattaaagtg tgccgataga aacttgcagt ctaaacaata cgcttcatct
     6901 tgatttaagt gttttaagca ccttgaacat cgccttgctt taatatcaag tttaatattg
     6961 tcccaattct ctttgcatct gtcacataat ctattaggtt tcttgaacaa attataaatg
     7021 gttatatttt catataactt agcaccacaa ctcaaacaat tattcatcaa tccaacctct
     7081 ttttaatgct aatttgttca tcctttgaat ctctttttta gcttgaatca tattcatact
     7141 taccccttca tgaaaaaaca atacttttcc agtaggacat tctaattttc gtccaacacg
     7201 tccagcaatt tgtattaaag cctcttgagt atattgatgt gcatcgataa caacaacatc
     7261 caaatttgcc attgtaaatc cacgttctaa tattgtcgta gtaaaaatga catcgaaatg
     7321 tccattcctt aattgttcaa ctttttcaaa gcgaaaaaca tcctcgctat ggacgtatgt
     7381 taatttagta attttctgct tataaaccga aaatgtttta atcattgttt ctatattgtt
     7441 aaaaaacacc agtgtataac gttgattatt aatttgatct tgtaaaattc ggtataacat
     7501 tttctgaatc ttcttattat taagtttgaa ataacgatat ttaggaactg gaagtgattt
     7561 tttatgaaag cgagctggca atttaattat attttcgtgg ggaatctctg acagaagttg
     7621 tttcggtggt gttgctgtca tataaattgt tgcatgttca actttagaag atgacttcaa
     7681 tgcttgttgt aaatttttat ccattgataa aggaaaggca tcgacttcat caataaaaat
     7741 agtatcaaag tgctgtttga atcggtaaag ttgatgcact gtgcatacaa caaaatgccc
     7801 ttcaaattgt tgtcttgatt gctggtgtag tatgtctata tcttcattaa gaaatgcgtc
     7861 tttaatacgt ttactaattt ctacaacaac atctacacgt ggtgacacaa tagctatatt
     7921 atctccctgt attcttgcat attgaatgcc ttgaaacatc atttctgtct tacctgcacc
     7981 tgttacggca tataacaaaa tcgtttgtct ctttctaatg gctcgaacaa tatgttctga
     8041 ggcataagac tgctgttccg acaattcaaa tgagagatga taatatgctt gtgatgaact
     8101 ttgcgaactc tctgttattt tatatcttgt tacattatcc attcgaccca gtgatataca
     8161 atttctgcaa tatacaattt tcttggaatg tactgaggaa tcatactgat aaaaatgctt
     8221 tggatttgta ctttcacatt gaacacaata atagtttgaa ttttgtttga tgacacctgt
     8281 tacttctttt ttaatttttt cattagtaag tgaagtatga ggatgaatta atttcccgta
     8341 atgtttaatg cttaaaacct catttcaacg taaaaaagag cctaatatta taatgatttt
     8401 cttatatctt tagcattttg gctttcaaat gatgaacatt taaagccatg tatacgagag
     8461 ataatttcca attacaatat agactctttt attaaaattt tataattatg ttaatcttat
     8521 ttttctgcca acatagccta aacctaatcc accagaacct aaatgtgctg caacaactgg
     8581 accaaactca gagtatgcta cttgataagc tgaaggacaa tcatcttgta actttttgta
     8641 taacgcttga ccatcttcga aatgatctcc atttatgaca aataaagtta cttcttcaaa
     8701 gtcttttaca atatctaata ctttcttttc taatgtttga atggcacgct ttttagtacg
     8761 aactttttct tctggtataa tcttgccatc ttcaaactta agaactggct tcattttcaa
     8821 taatgttcca acccatgctt gagcaccagt aattcgacca cttttttgta aattctttaa
     8881 gtcatcaaca attaaatatg cacctgtgtg ttcacgcata ttagttaaat catcaataat
     8941 ttgctgtggc tcgtatcctt cttcaaccat ttcaatagca cgtaatacat aacatccttc
     9001 aatcattgct gcaagcttac tatcaaatgc atgtacatta acaccttcaa ccatttcccc
     9061 tgcttgataa ctagattgat aacttccact aatcccactt gataagcaaa tgacaatgat
     9121 atctgtgtaa ccttgatctc ttaacatttc atatttagta atccattcgc caattgctgg
     9181 ttggcttgtt gttggaatcg tttgagacga tgccatttta ttataaaatt cttcaattgc
     9241 tatttcatta ctttctgtaa agttcttccc atcatcaaaa gtcacactta atggcgctat
     9301 ttgaatatta tatttatcga ttaagtcctg cgacagataa cttgtagaat cggtcatcac
     9361 agcaattttc atagtcgtcc tcctaaaata tactcatagc aatgatacaa tatttcagtt
     9421 aaaaagaaaa tggcaatcaa acgatttata gtcatgtcat tttcagtgta taatgaagat
     9481 taaataatct taagcttatg aattaaggag ttattatgac acaaaatatc attactgtta
     9541 aaaaagaaca cattattgaa aatgtgatta gtaaatcaag attcattgct catatcaaac
     9601 ctgttcaaaa tgaagatgaa gctaaagcat ttattgctgc aataaaaaaa gaacataaag
     9661 atgccactca taattgttct gcatatacta ttggacctga aatgaatatt caaaaagcga
     9721 atgatgatgg tgaaccgaca ggaactgccg gtgtcccaat gttagacata ttaaaaaagc
     9781 tcgatgttca taatgcgtgt gtcgtagtaa caagatattt tggtggtatt aaattaggtg
     9841 gtggcggttt aattcgcgcc tatagcggtg ctgttagaga cgtcatttat gatatcgggc
     9901 gtgtcgaact acgagaagca gtaccatgta tagtaacact gaattatgat caaactggta
     9961 aatttgaata tgaattagct tcaactcatt ttatgttaag aaaccaaact tacactgata
    10021 aggtaagcta ctatatcgat gtcgttaaaa gtgactatga agcgtttatt aactttttaa
    10081 atcaaatgac tgcaggaaat ttcgatttga ctgaagaaac tccaaagcag ttacctttcg
    10141 atataccaac tgaataaaaa taaaaagcac tatcataaga cggtaaatat caattgcgta
    10201 ttattaaacg acaattgaag tacacgttta tggcagtgct ttttatattt gaaatttaat
    10261 acaaaaaaat tgtaaagagt aaagccatat aatacaaatt atcccataca gctatgcttt
    10321 cattccctat tcctctttat gagatgactt acgactaatt aaatttaata ttggtcggta
    10381 gttgttatct attaatcctg taaattcaac aattaattca atactaaata tgattaatac
    10441 aaacattaat acaacaccta atggtggcga tacatacaaa ataatgctcg aaagactaaa
    10501 taaaatagag attgaataga ttaataatac tgtttgtcta tgtgtgtagc ctaaagctaa
    10561 tagtttatga tgcaaatgcg atttatcagc ttgcattata tgctgccctt ttttcacacg
    10621 tcgaatcatt gcgaacaaag tatcaatgaa tggaactgct aagataacaa ttgggaagaa
    10681 caatgcaata attgtaatat ttttgaatcc gagtaaagaa aggaatccga tgataaatcc
    10741 aatcattaaa gccccactat cacctaaaaa tattttggca ggatggaaat tgtaaaataa
    10801 aaacccaatt aaagagccta ataaaacaca acaaatcatc gttatgaaaa tattagcttg
    10861 taaaattgca atgaacccta ttgtaatgag tccgattgca gaaacacccg acgccaaacc
    10921 atcgagtcca tcaattaagt taattgcatt tgtaatagca acaatccaaa tcacagtaat
    10981 tggaatacta agaaatccaa aatgaatcgt tgtacccatt ggcaacgaaa taaaatcaat
    11041 agtcacacca taaaaagcaa ctactaaggc agcggcaatt tgaccagcca attttatata
    11101 cggtttcaaa tcgtagatat catctacaag cccaagtacg tacataataa tcgcaccaat
    11161 aataagtggt ttgatttctg tttcaatagg atgaccaatc caaataccta ttaaaaatga
    11221 aaagagaatc actgtaccac ccataactga aataggttta gtgtgtactt ttctaaaatt
    11281 tggtttatca actaaattta atcttttcga tattgcaata acaattggtg ttatcgtcaa
    11341 actgacaatc attgttactg caactagtaa taatgtaacc atttattcac cttcatcgat
    11401 attaattgtt tttaacgtta tatttattta atattgtctt tatagaatga tatatctatt
    11461 ttctacgtat atcttatagc tttattataa attaattcgt tcaatcaatg atatcatatt
    11521 tacaactata cgattcattt cttatatatc gcctattatt cattttttca tcatatcatt
    11581 taaggcaaca ttttaacatc aaagtctata tcatacatta attttatatg taaactaatg
    11641 acaaattgta agttttcact tacaatagct agtggtcaat taaaggagtc gattcagaca
    11701 tgttcgaagc atttatatac aatatatctg ttatagtcgc tggaatctat ttattccatc
    11761 gcttacagta ttcagaaaac aaacgtatgg ttttttctaa ggcttatgtc actgttttaa
    11821 tgacaatcgt atcattatta ttatctgtct accctatccc ttatcgtgaa gattatttaa
    11881 ttcacttaac attcgttcct ttattgtttt taggtagatt tacaaatatg gtctatacat
    11941 tatctgccac agtaattgta gcaattgttg aaattgtagt atttaacaac tcgattatgt
    12001 acggtgttac gttgattgtt attgctgcag taacaagtgc tattggacca tttttaaaac
    12061 aaaatgatgt gctatcatta ttaatactta atgttgttac aataatcatt ttatttggtg
    12121 tcgcgttagt gagtcctatt tacacactat cggaagttat cattttaata ccaatttcat
    12181 taattatcac tttagcttca gcgataacat ttgtcgatat ttggcatttc ttctcactcg
    12241 taaatcgtta tgaaaacgaa gataaatatg attacttaac aggcttaggt aatgtaaaag
    12301 aatttgatag acatttaaat gaaatttcac gaaaagctga aaaagaacat caaagtatcg
    12361 cattattatt aatcgatatc gatggattta aagatgtcaa tgatacctac tcacacaaat
    12421 caggtgatgc tgtattaaaa caaatgtctc aattacttaa aaactatgtg ccgaatcaat
    12481 ttaaaatttt tagaaatggt ggcgaagagt tctcagttgt cattcacaat tattcacttg
    12541 atcaaagtgt taaattagct gaaaatattc gttcgggtgt tgaaaaatca tcattccatt
    12601 taccaaacaa agaagttatt aaattgtcag tgtctatcgg tgttggttat ttaacagatg
    12661 acgatccaaa atcacagcgt aaagtgttta aagatgcaga cgatatggtg catgtagcca
    12721 aaaatcaagg gcgaaacaaa gtaatgttta accctattat caatttataa caacgacaaa
    12781 actgttaatc ctttgctatt attaaatcat agtataatag caaagattaa cagtttttct
    12841 tatttaaatt tgaaccatac aagttcatca tactaacaca aagtctcact attgaaagtt
    12901 gtaacaacaa gttgtaaact tcaattgttc aaataaataa cacatatcat tggaggtgaa
    12961 ttgatatgcc agattcaatc acaattatag atgaaaacaa agtgattgat gttgtattaa
    13021 ttgcaggtag aattttactt gaatcaggtg ctgaaacata tcgagttgaa gatacaatga
    13081 accgtatcgc acatagttat ggtcttcata atacatatag ttttgtcagt tcaactgcaa
    13141 ttattttttc attaaacgat cgaacaagta caagattaat tcgtgtacaa gagcgtacaa
    13201 cagatttaga gaaaatcgct ttaacgaata gtctttcacg taaaatatca aataaagaac
    13261 tcacaattga tgaagctaaa tctgaattta ttcatttaca gcatgcatca ttacaatatt
    13321 cttttttaac gaatttcttt gcagctgcca ttgcatgtgg cttcttccta tttatgtttg
    13381 gtggtgtcgc atcagattgt tggattgcag tcattgctgg cggatccgca tttttaacat
    13441 tcagctttgt gcaacgctat atacaaatta aatttttctc agagtttgtt gcagccgctg
    13501 tggtcatatc cattgcagcc acatttacta aattaggcat tgcaaccaat caagatatca
    13561 ttactatagc cagtgtcatg ccacttgtcc ctggtatatt aattaccaat gccattcgag
    13621 atttattagc gggtgaatta cttgcaggta tgtctcgcgg tgttgaagct gcattaacag
    13681 cattcgcaat cggtgctggt gtcgcaatcg ttttattaat catttaagga aggactgtta
    13741 aagcatgcta ttttatttat ttcattttac aatcagcttt atatcaacag tacttttctc
    13801 tatcattttc aatgcaccca aacgcctctt agtagcatgt ggatttgtgg gtgccattgc
    13861 atggacgatt taccaattaa cggtagattt agagtttgga aaagttggcg cttcattttt
    13921 gggaagctta attttaggct taatgagtca tactatgagt cgcagatata aacgaccggt
    13981 aattatattc atagtgccag gcattatacc attagtacct ggtggtgcag cttatcaagc
    14041 gactcgtttt ttagtatcaa atgattatac aagtgctgta aatacatttt tagaagttac
    14101 actgatttca ggtgcgattg ctttcggtat attagtttct gaaattctat attacctata
    14161 cacacgtatc aaacaactgt atggtaaaat caaaggtaag acatataaaa aatcttacaa
    14221 catgaataat agagtttaag gggtcgaata taatgaagaa ccaattgata gatagattaa
    14281 caagatatac gacaattgat acgcaatctg atccaaaatc tacaacaaca ccttcaactg
    14341 aaaagcaatg ggatttgtta catttattag aaaaagaatt acaacaatta ggattgccaa
    14401 ctgatttaga tgaaaatggc tacttatttg ctacattaga aagtaacatc gatgtcgacg
    14461 tcccaacagt tggatttttg gcgcatgttg atacatcacc tgatttcaat gcttcaaatg
    14521 ttaaaccgca aattattgaa aactatgatg gtaagccata caaactaggc aatactaaac
    14581 gtgtcttaga tcctaaagta tttccagaac ttaacagttt agttggtcat acattaatgg
    14641 taactgatgg tacatcgtta ctaggtgcag acgataaagc gggtattgtg gaaattatgg
    14701 aagctatttg ttatttacaa gaacatccag aaattaaaca tggtaccatt cgcattggat
    14761 ttacaccaga cgaagaaatc ggtcgtggtc cacataaatt tgatgttgac cgcttcaatg
    14821 ctgattttgc ttatactatg gatggtagtc aatatggaga attacaatat gaaagcttta
    14881 acgctgctga agcggttatt acatgccacg gtgtaaatgt tcatcctggt tcagctaaaa
    14941 atgcaatggt aaacgcaata cgtttaggtg aacaattcga tagtttgcta cctgatagtg
    15001 aagttccgga gcgaacagaa ggatacgaag gcttttatca cttaatgaac tttgaaggaa
    15061 ctgttgaaaa agcaactttg caatacatta ttcgtgatca tgataaaaaa caattcgaat
    15121 tgcgtaagaa acgtatttta gaaatacgtg acgatatcaa tgcccatttt gaaaattatc
    15181 cagttaaagt tgatatatcg gatcaatatt tcaatatggc agaaaaaata ttaccattgc
    15241 ctcatattat tgatatacct aaacgtgtct ttgccaaatt agatattcca gcaaacactg
    15301 aacctattcg cggtggtaca gatggttcac aattgtcatt tatggggtta ccaactccaa
    15361 acatctttac aggttgcggt aacttccacg gcccatatga atatgcatcc attgatgtta
    15421 tggaaaaagc agtacaagta atcattggaa ttgtagaaga tatcgctgaa aatcactaaa
    15481 aaccaacgat tatatactag ttcaactcag gcttaattaa tatataaaaa tcacgctaac
    15541 attcagtgca atgatagcgt gatttttcat attttttacg tatactttcg atcttctaat
    15601 tcgttttgct aaccatcata aatgttatta accatcatga cagcatcacc aatttagtcc
    15661 ttctttacta aacctcaaca ttaaaatgtg ttttcaacag ttttaaagac tgaaccgtat
    15721 aatgcctaat ttgtaacccc attttaaagt cagtataagt ttctggcata tcgataaatg
    15781 tattaaacat tgctgtaacc ccttgtgatt caaataaatc aaacttttca gcagttgcac
    15841 aaattgcaat ggcaaccttt tgatgtttat gacaaagttc tgcaatacgc aatgttgtcg
    15901 tttctagcaa ctgatcattt tcatttaatc cttctccaaa aataattaaa tccgcttgtt
    15961 caactaaatt ttctaaatgt gttagttggt ctactaatgc atgactggtt aatatttcag
    16021 cttgatacag tccattcaag actgctgcaa ttccaccacc agcaccacca cgttcaactg
    16081 gaccaattgc aattttcaat tcacttttaa ataactcact aaaataccaa attaaattat
    16141 cgatttctgc tgcttgatta tgattcaact gatacgcatc ataagtttgc atgatttcac
    16201 tttgcttacc atataatcga cttgaaaaat ccgacattac ttgaattctt gctgtttcca
    16261 ttttagggtg taagttcgac atatccatac gacgaatata tttaattaca ccagcacctt
    16321 gtctcatatc tacgacacgc ccttcgtcat catagaattg agcacctaat gcttgtaaca
    16381 tacctgcacc agcatcaaaa ctatcaatcc cacctagtga aattacaaca tgttttgcgt
    16441 cgttatctaa tgcatgttta atcatttctc ctaaaccata acttgttcgt tcaacaattg
    16501 gttttttacc ttttaaaaat aaattcccct cgataacggt catccctgta tcagtttgtc
    16561 cgtaaacacc ttcaacttca ttcatatctg catcatgtac tggtatacga tacttttgcc
    16621 cagattgcca taaaaataca gaatctaata attcatgtct tccattaaac aatggtactt
    16681 gaactacatc tgcagtttca atttggcttg caactgcctc ttcaacatat ctattagctt
    16741 gataacttga aataattcca tgaaactcat ccatggctac taatactttc ataaccttga
    16801 ccaccttact ttattatttg ttgtgatacg ttttttattc ataaaagatt aatcttaaat
    16861 tttagatttt atctcgtgat tatatgcgaa catttaacat gacatgatta ttaattattc
    16921 cagtaaataa caatatgctt cttttggttt agcatatatt tcctattaaa ctaacttaca
    16981 aatcatgttt aaattttctt attggattcg tcgttttcca aaatattttc aataaaaata
    17041 ctcgaatata ttatacatat gtttgtaaaa aagtactaac ttaactgctt ctaaaggtga
    17101 cataaaataa aaggtcagaa catcattata gccaaactac tctgattcac acgaatttaa
    17161 gttgtgctat tcacgtctag tagtgtaagg caaaatgttc caaccctata gtttcattat
    17221 tcttctgctt gtgctaatga cgacacattg atatcaccgt ggtctcgatt ccaaaccatt
    17281 tcaccattta caaaataaaa tgcttgaggt gattcatgtt taacgttcgt ttttttagca
    17341 atataatctg acaaatcgcg ttcttgttgg acaatcaaat aataaccgtc catatcgcgt
    17401 tcatataaaa atttattaaa ttgatcgtac gcatttgccg atattggaca agtttcacta
    17461 tgttttaata caaaaacata tttattttcc tcaataacct gttcaaattg gtcaattgaa
    17521 cttagcttta tagccactct attcacctct aaaaatattt atcacattta aatagcatct
    17581 gcgtgacatt tttatacata tagttaatat caatgtttac cattatacac atagtaagtt
    17641 gacatgttaa ccataaattc aaaattttta actttgaaaa atgattatag ttatttttct
    17701 tctaattttt caagcttttt ttcaaaggct ttatcgccgt gagttatatc tatacccttt
    17761 ttcggcaact tatctacatc gacttttgat aacttctcac taacctttat tgtttcaata
    17821 cgtgtattat aatagttctg cagactgtaa tacatttcta ttgcgttttt ccttgcatta
    17881 ttaacatgct tatcactaat attagtttgg ttaatatcgg taatttgctt ttcaatcatt
    17941 ggcataatgt gatttttaat cgcgcctttt acttcatttt ctttactatc atctaggttc
    18001 ttcttcgcag tatctcttaa cgctttatta ttatgttcta attttgtcgt aagattagtt
    18061 gcttcacttt tattatcagc taatttaatt tctgattcaa ctttgtatct atttttttca
    18121 aattgtttcg tataatctaa aatgtcttcg ttatacttga tagattgatt acataaacct
    18181 atgaattttt ttaattgata tatcgcatcc ttcttctcat ttgcaagcgt catatattct
    18241 ttttttaatt tcttaacttt cattgtatca tcaggcaaat tttttgctga cttataatat
    18301 gcttcaaatt taggtatgag atggttttta acatcttctt gtagtgcctt aaattcttta
    18361 ctatttttat cagttgtatc agttttactt agatgatcaa tttctttcaa atgtatatta
    18421 tccatgacat gttgtaattc tttttgtttt tgctcaacat ctttaaattg cttagtaaat
    18481 tgatgttgtg cctctttttc tttattacca caagcactta ttactacaaa taaaatcgct
    18541 aaaacagcga taataacaat ttttttcatg attgcactcc ttaacagatt cattatacaa
    18601 aaacatgttt attatgaaaa tgaattatgt tatagtattc cagactttat cgtgattaaa
    18661 ttaattttca atatcaaagg agtttatcat gtattcaaat gttcaaccat tcattaatag
    18721 tgatttaact aattgttttc aatcacaata cactgaaatt gaatcattat tattcaacct
    18781 cttagacagc ccagtaaaat atacaaaaca tattggcggt acgcatcatt ttaaatattc
    18841 aacagagcca attttagata ttttagtagg tgtagaaaat ttgcatgata ttacatcctt
    18901 agatgaaaaa cgcttaaatt atgtagggtt ttatcgtttg catcataaat atactaagaa
    18961 agttatgatg gcgaagttta acaattttca tgaactaaaa caacaggtgc gtctacatat
    19021 cattcaaatg aatacccaaa catttgaatc ttatcttgca cttgattacc tcttatcaac
    19081 taatgatgat attgctttgc aattcaatac aaaaaaacac caaatattgc aacatagctt
    19141 aacaattaga caatatgaga tgcaaaagca acaatacttt gaaaaactat gtaaaaaata
    19201 cttataacga caataatgcc gtattttata attatactaa ttctaatact ttcttttcaa
    19261 ttt
//
LOCUS       DAKTGS010000040        19070 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.040, whole genome shotgun sequence.
ACCESSION   DAKTGS010000040 DAKTGS010000000
VERSION     DAKTGS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.040"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(157..258)
                     /locus_tag="O0286_001188"
     CDS             complement(157..258)
                     /locus_tag="O0286_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169572.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            316..756
                     /locus_tag="O0286_001189"
     CDS             316..756
                     /locus_tag="O0286_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0169573.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            743..2065
                     /locus_tag="O0286_001190"
     CDS             743..2065
                     /locus_tag="O0286_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCY0169574.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(2117..2458)
                     /locus_tag="O0286_001191"
     CDS             complement(2117..2458)
                     /locus_tag="O0286_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCY0169575.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(2635..3711)
                     /locus_tag="O0286_001192"
     CDS             complement(2635..3711)
                     /locus_tag="O0286_001192"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCY0169576.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            complement(3724..4707)
                     /gene="mvaD"
                     /locus_tag="O0286_001193"
     CDS             complement(3724..4707)
                     /gene="mvaD"
                     /locus_tag="O0286_001193"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCY0169577.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            complement(4712..5632)
                     /gene="mvk"
                     /locus_tag="O0286_001194"
     CDS             complement(4712..5632)
                     /gene="mvk"
                     /locus_tag="O0286_001194"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCY0169578.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            complement(6219..7055)
                     /locus_tag="O0286_001195"
     CDS             complement(6219..7055)
                     /locus_tag="O0286_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0169579.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(7058..8044)
                     /gene="pta"
                     /locus_tag="O0286_001196"
     CDS             complement(7058..8044)
                     /gene="pta"
                     /locus_tag="O0286_001196"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCY0169580.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            8212..8964
                     /locus_tag="O0286_001197"
     CDS             8212..8964
                     /locus_tag="O0286_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCY0169581.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            9513..10277
                     /locus_tag="O0286_001198"
     CDS             9513..10277
                     /locus_tag="O0286_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0169582.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            10290..10742
                     /locus_tag="O0286_001199"
     CDS             10290..10742
                     /locus_tag="O0286_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0169583.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(10884..12368)
                     /locus_tag="O0286_001200"
     CDS             complement(10884..12368)
                     /locus_tag="O0286_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0169584.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(12489..12857)
                     /locus_tag="O0286_001201"
     CDS             complement(12489..12857)
                     /locus_tag="O0286_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCY0169585.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(12990..13370)
                     /locus_tag="O0286_001202"
     CDS             complement(12990..13370)
                     /locus_tag="O0286_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCY0169586.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(13371..14027)
                     /locus_tag="O0286_001203"
     CDS             complement(13371..14027)
                     /locus_tag="O0286_001203"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCY0169587.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            14211..15041
                     /gene="thiD"
                     /locus_tag="O0286_001204"
     CDS             14211..15041
                     /gene="thiD"
                     /locus_tag="O0286_001204"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0169588.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            15548..15715
                     /locus_tag="O0286_001205"
     CDS             15548..15715
                     /locus_tag="O0286_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCY0169589.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(15916..16185)
                     /locus_tag="O0286_001206"
     CDS             complement(15916..16185)
                     /locus_tag="O0286_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169590.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(16188..16553)
                     /locus_tag="O0286_001207"
     CDS             complement(16188..16553)
                     /locus_tag="O0286_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCY0169591.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            complement(16528..17667)
                     /locus_tag="O0286_001208"
     CDS             complement(16528..17667)
                     /locus_tag="O0286_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0169592.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            complement(17669..19045)
                     /locus_tag="O0286_001209"
     CDS             complement(17669..19045)
                     /locus_tag="O0286_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0169593.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
BASE COUNT         6074 a         3561 c         2890 g         6545 t
ORIGIN      
        1 tcagttgtta aaagtatttc ctcatcatgc gaaatatcaa aacgtatcta aaatacgaat
       61 aagtttatac aatcacacaa catcatcatt caaaatttta ttgctctcat tttcaatatt
      121 catttcatat ctatcagttc atacataatt aaaatttcaa ttgtgcaatc tcaccgttaa
      181 tgcatcaact ttcaaacaat aaatcatcac aataaccaca cctaattcaa cacttttcaa
      241 acataagtat tgacacattg agcaaaatga tttttaattg taactaatac agttacaatt
      301 atgaggtgag aaacattgaa tttagaattt aacattgccg tgcatgtatt agctttttta
      361 actaagcatc attcagaaaa attcaatagt agttcattag cagaattaac ttgtttaaat
      421 cctgttcaat tacgacgcgt gacgactcaa cttgtcgatt taaaaatgat tgacacaata
      481 cgaggtaaag atggcggtta tttagcaaat gatcaaagtg ctgatgtctc tctagcaaca
      541 ttatataaac attttgtctt agagaaagaa caccacacac gtctatttac tggcgacgaa
      601 ggcagtcact gtcaaattgc tcgtaatatt gcaactacca tgtcacatta tcagcaagac
      661 gaacagaata tcattattaa tttttataat ggaaaaacaa tcaaagatgt cattgaagac
      721 attcaaaagg aggatttatg tcatgaaaac atatgattta attgtaatag gatttgggaa
      781 agctggtaaa actttagcga aatacgctgc atcaacaggt caacacgtcg cagttatcga
      841 acaatctccg aaaatgtatg gaggcacttg tataaacata ggatgtatac cttcgaagac
      901 acttgtacat gatggattag aaggcaaatc ttttgaagca agttataacc gtaaaaacga
      961 tgttgtcaat gcgctaaaca ataaaaatta ccacttatta gcagacgata acaacattga
     1021 tgtactggat tttaaagcgc agtttaaatc taatactgaa gtgaatttat tagatcaaca
     1081 tgacgatatc gtcgatagta ttactgcacc tcatatcatt attaataccg gtgctacctc
     1141 tgtcattcct aacattaaag gccttgatca agctaaacac gtcttcgatt cgacaggttt
     1201 attaaacatt agctatcaac ctaagcacct cgttattgta ggtggcggtt atatcgcctt
     1261 agaatttgct tcaatgtttg cgaatttagg tagtaaggta acagtattag aacgtggcga
     1321 atcatttatg ccacgcgaag atcaagatgt cgttgcatat ggtattactg acttagaaaa
     1381 taaaggcatt gcattgcata caaatgttga aacgactgaa ttgtcatctg acaatcatca
     1441 tacaacagtc cataccaacg ttgataactt tgaggctgat gcagtacttt tggctatcgg
     1501 gcgcaaaccg aatacggatt tagctttaga aaatactgat atcgaattag gcgacagagg
     1561 cgaaattaaa gtcaatgctc atcttcaaac aactgtgccg catatttatg ctgcaggtga
     1621 tgttaaaggc ggacttcaat ttacgtatat atctttagat gattatcgaa ttatcaaatc
     1681 agcgttatat ggtaatcagt cacgtacgac tgacaataga ggcagcgtgc cttatacagt
     1741 atttatagat ccaccattat cacgtgttgg attaactagt aaagaagctg ccgctcaaca
     1801 ttatgattac actgaacatc aacttttagt aagtgctata cctcgtcata aaattaacaa
     1861 tgatccaaga ggtttattta aagtagtcat taataatgaa aataatatga ttttaggtgc
     1921 tacattatat ggtaagcaat ctgaagaatt aattaatata attaaacttg cgattgatca
     1981 aaacattcca tataccgtat tacgagataa tatttatacg catcctacga tggccgaatc
     2041 atttaatgat ttatttaatt tctagacaaa acataaaaac ctggtggcac gcattgaatg
     2101 atgctgccat caggctttat tgttgtgctt ttcgcttttc taatttttct ttaagctttc
     2161 tatcttgttc tactttacga cgtttacgtt cttcatgtcg ttttcttaaa cgctcttctt
     2221 cttcaggatc acgtggtttc tttaattgtt gagaaacttt ttcgattaat tcttcttcag
     2281 taagcgcagc cagtgggcgg ttattaacaa aagtgaatgt ttttcggcgt ccaggtccac
     2341 aataagattg acaacctatc acgatttcag catcgggatc taatttttcc aacttctttt
     2401 gtaacgttct tatattgact gcctgacatt catcacaaat aaggaatgta tttttcatat
     2461 tgctacccac ctttctttat catatctata tcgtcgattt cattaatttt ttcgttaact
     2521 ctatctattt tactcttttt aatatttttt tcaagatacg taacacggct gacaataaaa
     2581 aatggagcat ttatcttcta attaaattag atgattgctc ccctatcaaa tcatttattg
     2641 cccatgataa atattaaatt ttaatggttt aataccatgt tttgtccatt catcataaat
     2701 tttttcttta tctacatctt tattgataat tgtaatacca cagtctccac caccagcgcc
     2761 tgatgtttta gatgcgccgt gatacttttc agcaatatca cacaaatatt ttagcttttc
     2821 agtttctata tcaactgtag cttctttatc catacgttga ataattgtac gattctgacg
     2881 caccatcttt tgcacacctt taatgttatt tgttttaaaa gcatgaataa gtttttcaac
     2941 acaacgatgt gaatcttcta agaagtcacc gtaaaatgaa ggatctgatt tcaaacgttt
     3001 cacttcgcta acaaagtgtg gtgatgacgc cggtgagcca gtccaaccga taagtacttc
     3061 catattttca ggtgcttgta atggttcgat gtgcaatcca ggccagtttt tgattaaaac
     3121 ttcttcaacc gtagtatctt caatttgatg cttaacccat tcatgatcaa aagtactata
     3181 cgctagccat ccactatata cactcacagc aatatctccg catgaactta aactttgtaa
     3241 cttcatattt gcaatcactg ctagtttata aatgtataaa ttagataact tcatatcata
     3301 aaattcattt aatactttta taactgacac aagtactgct gcacttgaac ctaatccata
     3361 tttatgacca tttgaatcat ctaaattact atcaatagtc agatgaaaat gcttcatcgc
     3421 tatatcgcaa cttttcgcgt attgttcaaa tatttcaata gctgtgacca cataatttaa
     3481 ttgttttgct gcatgtggat ctgaaatgac aatactatct tcatctctac taaatgtaac
     3541 tgggttatga tgtaatgctt ttgaatgaat ggtaccttta tattggtctg tttcttcaat
     3601 agtagcagtt acaaaacgat ctaacgcaat aagtacagat ttatatcctg gttctgttac
     3661 agcatattct ccagcaatat aaagttttcc gggtgctttg acctgaatca ttttatctct
     3721 tccttactca attatttcaa ttcctgtggc aataatgtca ctatcaataa tttggttatt
     3781 atcaaactgt gttaataatt tatctataat ctgttgcttg tttttctttt ctacaagtat
     3841 tttcacatta ggtcccgcat ccattgtaaa ataacacgga taccccgctt ctcggcattc
     3901 gtgaacaagc gccatgacat cataactttc ttgcacaaga tatgtgaacg gcggtgttga
     3961 tcctagattc gtggcatgca tacgcaaacc attttcttca attacttcac caaggcgttt
     4021 aaaatctttg tcttgaatcg ctgcttttgc ttcagctaaa tcttcatcaa tatgatctaa
     4081 ccaatattga taaaaccttg atgtgtttcg tgtcaatgac ataccatatc gactaggtac
     4141 ctttttagaa tgttgattaa tcacaacaaa tatcatggca aggtcatctt caaaatgatt
     4201 cgattcaagt ggaacggcat atgacgtctc atcactatac cctttttccc attctgcaaa
     4261 tccaccataa atactacgcg acgcagaacc cgaaccaatt cgcgccaatc tcgataaatc
     4321 cttatctgac agctgcatgt ctagcgcttg attacaagct gctgctaaag ctgcatatgc
     4381 gcttgccgat gaagccaacc ctgctgctgt tggtacaaaa ttgtcgcttt caatttctgc
     4441 ataccaatcg atgccagctc tatttctgac aatatccata tattttgaaa ttttctctaa
     4501 ttctttgcca ctaacctttt caccattcaa ccaaaattga tcctgtgtta actggtcgtt
     4561 aaaagtgact ttcgtttcag tgtaaaattt ttctaatgta acagatatgc tattattcat
     4621 tggaatgatt agtgcttcat cttttttacc ccaatatttt ataagtgcaa tattcgtatg
     4681 tgcacgtgct ttgccacttt taatcaacgc attaacctcc taaattctca atccaagtat
     4741 gtgctgcacc agctttttct acagctttta caatattttt cgctgttggt aaatctttgg
     4801 caagcaataa catacttcca ccacgaccag cgccagtaag ttttccagca atcgcaccat
     4861 tttctttacc aattttcatt aattgttcta ttttatcatg actaactgtc aacgccttta
     4921 aatccgcatg acattcatta aaaatatccg ctaaggcttc aaagttatga tgttcaatca
     4981 catcactcgc acgtaaaact aacttaccga tatgttttac atgtgacatg tactgagggt
     5041 cctcacaaag tttatgaaca tcttctactg cttgtcttgt tgaacctttc acaccagtat
     5101 ctataacaac catatagccg tctaaactta acgttttcaa cgtttcagca tgaccttttt
     5161 ggaaccaaac tggtttgcct gatacaatcg tttgcgtatc aataccactt ggtttaccat
     5221 gtgcaatttg ctctgcccaa ttagcctttt caatgagttc ttctttcgtt aatgatttcc
     5281 ctaaaaaatc ataacttgca cgaacaaaag caaccgcgac agctgcactc gatcctaatc
     5341 cacgtgatgg tggtaaattc gtttggatcg ttactgctag cggctctgta atattattta
     5401 attctacaaa acggttcacc aaagacttaa gatggtcagg cgcatcatat aacataccat
     5461 cgtaaacatc gcttttaata gacgaatagt tcccgctctc taaggcttct attaaaactt
     5521 tgattttacc tgcgttaaac ggtactgcaa tagcaggctc tccaaatgta acagcatgtt
     5581 ctcctattaa aataatctta cctgtcgatt ccccatatcc ttttcttgtc atgtcaatat
     5641 caccttttat atttatccta tacttgattc attattttta tttattagta aaagacatca
     5701 tattctaagt tgcatacgca ttctcgttaa atttcattgc agtctttatc tcacattatt
     5761 catattatgt ataatcttta ttttgaattt atatttgact taacttgatt agtataaaac
     5821 taactttcgt ttacttcaaa gtttaaatct tatcgagtga tatttcagat tctttatctt
     5881 tttataaaat agccctacaa tttataattt tccaccctaa ctataatact acaaataata
     5941 attggaatat atagatttac tactaaagta ttagaacatt tcaatagaag gtcgtttctt
     6001 tcatagtcat acgcattata tataccctat tctcaatcta tttaatacgt aaaacatgaa
     6061 attttcttat taaatttatt atttccatca tatcattact tttaatttaa tgatgttcaa
     6121 tttaaatatt aggtcaataa catatttatg ctttttatgg atactttcaa aaataacagc
     6181 cccaaacgat aacttgaaag gggctgttaa atatttaact attgcatttg atctatcatt
     6241 ttcttgtttc tttcaatcat tttatcaaaa tacgtatcgt atctttgcca ttcttcttga
     6301 gtaattggcg tcatatttaa tacaccgcca agatctttga ttgcatataa tagtaaaaac
     6361 attacatctt gaacagtaat cgttttgtta agcaatgtct ctaatgaggc catacaagat
     6421 ggttcaattt caggatattt aaatttagtc acttcacctt ttaaagcatg ttcataaaat
     6481 gtttgcatca tcaatgcacg ttctgaacca gagccttcaa cacaaagata aatttgtaca
     6541 gcaataccgc ctctaactct tcgttgcgat atgcctgcaa atttcttacc atcgatactt
     6601 aagtcaaatt ttcctgggca ataagaatgt tcaatttcca tcgtatcaat atcaacattc
     6661 tcattttcga acattttgct aattaagagg tacatcacag taaacgcttc atcaatcgtt
     6721 gtttctgttt gtcctttgaa catcagcgat atatttaata caccttgatc tagaacgaca
     6781 cctaagccac cagaatttct aacaatggca ttataaccaa tctcattcgt taaataatca
     6841 atgccatctt ttaaaaacgg caatcttgaa tcatgaatac caagaataac agtatgttga
     6901 tgaatccaag tacgcacaac attatctgat atatctttgc ccacactttc gcaaaatgta
     6961 tcatcgaatg cgaaagattg cataggttct aatccagaag aatgatcgat atatcgccag
     7021 ttgacgccat taaaatattt actcgctaaa tccatcgtta ttgtaaggct tgcgctgctg
     7081 taataattga aagattgtat acatcttcaa ttgagcagcc acgtgataag tcatttactg
     7141 gagaatttaa accttgtaat actggaccaa ctgcatcata tccacctaaa cgttgtgcaa
     7201 ttttgtaacc aatattacca gcttctaaac ttgggaatac aaagacattt gcatcacctt
     7261 gtaatttagc acctggcgct tttttctcag caacacctgg tacaatcgca gcatcaaatt
     7321 ggaattcgcc atcaatgatt gcttctaatt tttcttcttc agctttttgt tgtgctaatt
     7381 tgacagcttc ttgaactttt gtcacgtcgt ctgatttagc agaccctttt gttgaaaagc
     7441 ttaacattgc aacttttgga tccatgccaa agcttaatgc tgattttgca ctttctactg
     7501 caatttctgc aagtccttgt gaatcaagtt ctggattgat tgcacaatca ccaaagatgt
     7561 attgttcatc acctttaatc ataaagaaga tacctgatgt tcttgataca cctggtttcg
     7621 ttttgatgat ttgtaaagct ggacgcacag tgtcgcctgt tgaatgtgct gcaccactaa
     7681 ctaaaccatc tgctttacca gcataaacaa gcattgtacc gaagtagttc acattgttta
     7741 ataattcttg tgcttgttct tcagtcgctt tacctttacg tcgttcaaca aatgattgaa
     7801 ctaattcagc tttcaattca cttgtcgcag gattaattaa ttcaatatta gaaatatcaa
     7861 gatcaagttt ttgcgctaaa gattgaacct tagtctcatc acctaacacg attggtgtaa
     7921 cataatctgt tgcttgtaat tgtgttgcag ctgttagaac acgttcgtcc tctccttcag
     7981 gtaatacgat tttaacgttt ttaccagaaa gtttgtcttt taatacattt aataaatcag
     8041 ccataatgtc ctcctgtaat ataaatctta ttaatcattc acggtataat tatacgccat
     8101 tttatttcat aatgccacgt caccaatttg taatcataat tttatcgtta cccctttgct
     8161 ttatgataaa atttataaag aactgatgat ttttgaaaag gagcgataaa catgagtcaa
     8221 gcagccgaaa cattagatgg ttggtatagt ctacatttat tttatgcagt tgattgggca
     8281 tcattacgta tagttccaaa ggacgaacgc gatgcacttg tcactgaatt tcaatcattt
     8341 ttagaaaata cagcaactgt aagatcatca aaatctggtg atcaagctat ttataatata
     8401 actggtcaaa aagcagattt gttattatgg ttcttacgtc ctgaaatgaa gtctttaaat
     8461 catattgaaa atgaatttaa caaattgcgc attgctgact tcctaatccc tacatattca
     8521 tatgtatcag tcattgaatt gagcaattat ttagctggta aatctgatga agatccttat
     8581 gagaaccctc atatcaaagc aagattatac ccagaattac cacattctga ttatatttgt
     8641 ttctatccaa tgaacaaacg tcgtaatgaa acttataact ggtacatgtt aactatggaa
     8701 gaacgccaaa aattaatgta tgaccatggt atgattggta gaaaatatgc tggcaaaatc
     8761 aaacaattta ttactggttc tgtagggttt gatgatttcg aatggggcgt aacattgttc
     8821 tcagatgacg tattacaatt caaaaaaatt gtatacgaaa tgcgctttga tgaaacaaca
     8881 gcacgatacg gtgaattcgg tagtttcttt gtaggacata ttattaacac aaacgaattc
     8941 gatcaattct ttgcgatttc ttaatacatt ggtacgttta taaattaata aaaaaattcc
     9001 aagcttatcg gtttaagctt ggaatttttc gtttatcttc agtatattcc cgtatacata
     9061 agacgtgatt tggtaaatag ttgaaatctg tatgtttaaa cttatatata tgtgctaatg
     9121 tattatcaat aacaaagtac actttgctca tagcaaagtg tacccgagta gtcttccttg
     9181 ggagaacttt aactactatc actacatata aacgttaacc tcaatagaaa ttatacagtc
     9241 gctactctat acaatttttg taatggttaa ctaatattat tttaacctat ttgaaatatt
     9301 tgaaacatat ttttgtcgaa tttttttcaa taatttttcc tttttatact tcaagagaat
     9361 tttaactact aaaaattccg atgattatta ttacaatagt atcaaatatt agttttttaa
     9421 aatcaataac aacttatcaa aaagctcatg tggttatttt atagtgtata aactataatg
     9481 agtattaaat tcttataaac aatggtgatg aaatggacat aaattcagaa gaatacaaac
     9541 aagaggtact tatcaaagac gttgtcatgc ttgctgctcg catactatta gaatctggtg
     9601 cagaaggtac gcgtgtagaa gataccatga cacgtattgc aaaaaaactt ggttacagtg
     9661 aaagtaacag ctttgttaca aacactgtca tccagtttac gttacattcg gaatcgtttc
     9721 ctagaatatt tagaattacc tctcgagata caaacttaat aaaaatttct caagctaata
     9781 aaatttcgcg tcaaattaca aacaatgaaa tttctttagc cgaagcaaaa acgcaacttg
     9841 aaaaaatata tgttgctaag cgtgacagca gtcttccctt taaaggtttt gctgcagcaa
     9901 tgattgcaat gagtttctta tatttacaag gtggtagatt gattgatgtt ttaactgcga
     9961 tattagcagg tagtctagga tacctagtca ctgagatttt agatcgtaag ttacacgcac
    10021 agtttatccc agaattcatt ggttcattag ttattgggat tatcgccgtt attggacata
    10081 cacttattcc aacaggtgac ttggcaacta ttatcattgc ggcagtcatg cctattgttc
    10141 ctggtgtatt aataacaaac gcaatacaag atttatttgg tggacacatg ttgatgttca
    10201 caacgaaatc attagaagca ttggttactg cgtttggcat cggtgctggc gttggtagcg
    10261 tattaatttt agtataggag tatcagacta tgttttggat cttaaacttt atctttagct
    10321 ttttagcttc aatgttcttc tgcgtcattt ttgatgcacc tagaaaatta tatctatcat
    10381 gtggtttcgt tggtacgtgt ggatggatgg tttacacctt attcttcaac ggctttaatg
    10441 tgcacactat atactctagt ttctttggta gtttagcatt aggcttgtta agtcattata
    10501 tggctcgtaa acaaaaagaa cctgccatca tttttatggt aacgggtatc attccattag
    10561 tacctggtgg cttagcatac gatgctacaa aaaatttagt cttattaaat ttcagtacag
    10621 caatcaatac catgctagag gttacactta ttgcaggcgc catcgcatta ggtttattat
    10681 tcgccgacca aatttccaaa ttaattgttt ctgggttcgt gaaatctttt aaacgattat
    10741 aaatatttgt tagaaatggc tcagccctaa ctcttagcaa ttatatataa ggttcagctg
    10801 cctcgtctta aatgtcaatt tataatacaa aaaatcgcac gatacgcctc cgaatgaaga
    10861 aggtttattg tgcgattctt ttattattta gttttttctt tttcaggttc ttcaatcata
    10921 tcatcaactt cattttcact tctatttaaa atcaagcgac ttaattcatc attatccata
    10981 tttttcaatt taggatatcg aatcacaaag aatattaaac cgatgattaa ccatccaagt
    11041 aatgcaatat aagacggtgc agtcagtgct gcaggagaac ctggcactaa taacaacgct
    11101 aagaaaatga atgatacaaa tgagccgata atagcaaacg ttttgtaaac cggtgcatac
    11161 gtattacttt gtttgttata actgaataat ttcgctgcag acaaacatgt aataaagtag
    11221 gcaatggata caccagtaga tgacatatct acaatccaag tcaatgcagt tcttcctagc
    11281 caaggtgcaa ttaacgacac tcctactagg aatatgattg cgacatatgg tgttttgtat
    11341 ttactatgta atttactaaa cattgttggc ataatacctg aacgtcccat agaaaataac
    11401 aagcgacttg aactcatcaa gaatccattt aaaccagtaa atatacccat cataattgca
    11461 attgctaata cacctaatcc aatataacca aatgctgttt gtgtaacagc acctgttaac
    11521 cacaactgcc catttaaact ttgatgactt gttgataacc aaccagtgta taaaatcatg
    11581 acaacataag ttaatgatgc tgctaataaa ctgtacacga taagcttaaa tgtcttgttt
    11641 ggtgcaaagt taaactcttc tgctgtttgt ggaatattat caaatccaac atatgcccat
    11701 ggtgccacgg atacaataac cacaatagac actaaccatc ctttgctagg ttcagctaac
    11761 ggttgtaaat tttcaagtgc aaaattatta ccaaagaatg aaccaaagaa catcaataat
    11821 acgacgatta ccatcgccac acagaaataa tattgtaatg atccagatac acttgcgcca
    11881 cgaatcgtta ctagcatgaa tacaagtagt aatacggtcg caataatgat ttccgtaata
    11941 taaacgtccc agcccgcaat ggtgtatagt ttcccattat ttaagacatc tggcaataag
    12001 aatttaacta gtaaactgaa tgcggtcgca tttaaagcaa cgacacagac ataaccaaaa
    12061 gttaaaaacc atgatgagaa gaaactcaca tatctgccga aacttaagaa actaaaggca
    12121 aacgcgcccc ctgatactgg aaatctctct actaatgcgc cataactaac cgcaattaat
    12181 atcattaata atgcaccaat aactatacca attgatgctg caatcggacc tgactgctta
    12241 atccagtctc ctggtaagat gaatgcgccc catccgatac atgaaccata tgcaatcgcc
    12301 catacaaact tttcagatag gttttgtttt aaatcgcctc tatctatttg cttattcttt
    12361 ttttccataa ataaaaactc acctcgaagg tattctatac ccaaaagatg agtttgtaac
    12421 tctttttctt gattattttt attaaaaatt taatgtttag ttacataggt aatctaaagt
    12481 tttaaaattt aaccagcaaa tttgaatgtc gcaatgatta acattatcca tccaatgatg
    12541 aacaatacgc caccaattgg cgtaatcgca cctaaaactt taatttgagt taatactaaa
    12601 atatataatg atccactaaa gaaaataata ccagcaaata ttaaccagcc agcccagtta
    12661 acattgattg aagttgtacc actaattaca cctataatta ataatgctaa gccatggtac
    12721 atttgatacg tcgttgcttt ttcccatact gataaatagt gatcacttat ttttccttgt
    12781 aaaccatgcg caccaaatgc acctgtaccg acagccatca tcgcgtttaa tgcacctaaa
    12841 ataataaata atttcattgc tacctctcct tatataattc agtattaata aatgtttaaa
    12901 tccgaacact cgaataaaag tatatgcgtc ttgattatta acctacatac ttaacaagct
    12961 attaagtaat tatgtttcat tgttgctttt taaaaatcaa atatggattc accattgcca
    13021 atgtcatcat ctgtcgctaa tctttgttgt tggtttgaag gttgtgaata tgacttatta
    13081 tgatgatgtg ctgaatgcgt atttacttta ccacccatcg cttcaatttc agcaactgtc
    13141 acttgatgag ttggctgcgt tatttgtgat tgtggcattt gattatggtt agcaatacga
    13201 cgattcattt gttgttcacc aatatgtggt gtatttgatg ttgaagcata taaagatgtt
    13261 aatatatgta tggcatacat atgtttttca aagtcatggt catgctgtgc ctgctctgct
    13321 tgtactaatt cttgctcaat tcgtgctatt aaagtttctc tattcaacat ctacgcctca
    13381 ctttcacacc aattaattgg tgattttcct actgactcta aataggcatt cgctttggaa
    13441 tacggttttg atccaaagaa tcctctatat gcagacagtg gactaggatg cactgatttt
    13501 ataatacaat gtttagatgt atcgataagc tttatttttt gctgtgcagg tttcccccac
    13561 aaaataaaga caacatgttc tttataatca gacactgctt taataatttc atcagtaaat
    13621 gtttcccaac caatatcacg atgagaattt gcttcaccct gtcttacggt taaaactgta
    13681 ttcaataaca agacgccttc tcttgcccaa tcttgtaaat gcggtgtttg tctaacgcat
    13741 ccaatatcat ctgctaattc tttatacata ttacgtaaag atggagggaa ttttgcgtta
    13801 ggttgcactg aaaatgctaa tccatgtgct tggtttggac catgatacgg gtcttgtcct
    13861 aatataacaa ctttgatatt ttcaaacggt gttaaatcaa acgcttgata tatattttcc
    13921 ctatcagggt atacgattgc agtcgaatat tctttttcta aaaaatcatg catagcttta
    13981 aagtcatgtt tcgttgttat gtcatgaaaa atttgcgacc attccattca atctcacctc
    14041 aggacatatt ttaacatatc acacttatct aaagtgaatg acattttcaa aagttacaat
    14101 agtatgaatg attcatttca acaatcatca aatattgaat gcttaatatt tttcaattag
    14161 gttaaaaatg atatgatgaa tacaatatta ttatgtagga gtgaagctca atggctttaa
    14221 agaaagtttt aacaattgcc ggttctgaca caagtgctgg cgcaggtatg caagcagatt
    14281 tgaaaacgtt ccaagaatta gatacgtatg gcatggtcgc tttaactgcc atcgttacta
    14341 tggataaaga tacatggtca cacgatgtta caccattacc aatggatgta tttgaaaaac
    14401 aacttgaaac ggctttatca atcggacctg atgctattaa aacaggtatg ttaggtactg
    14461 aggagataat caaacgtgct ggagaagtat atgaggcatc taatgcacaa tattttgtag
    14521 tagatcctgt catggtttgt aaaggcgaag atgaagtgct taatcctgga aatactgagg
    14581 cgatgattaa atatttacta cctaaagcaa cggtagtgac accaaactta tttgaagcgg
    14641 gtcaattatc aggattaggt aaattaaatt caattgaaga tatgaaaaag gctgcaacaa
    14701 ttatctttga taaaggcgca cagcatgtca tcattaaagg tggcaaagct ttagatcaag
    14761 ataaatcgta tgacttatac tatgatggtc aaacatttta tcaactaaca acagacatgt
    14821 tccaacaaag ttataaccat ggtgctggtt gtacatttgc tgcggcaaca actgcatatt
    14881 tagctaacgg taagtcaccg aaagaagctg tgattagcgc gaaagctttc gtcgcttctg
    14941 ctattaaaaa cggttggaaa atgaatgatt ttgttggtcc tgtggatcac ggtgcataca
    15001 accgtattga acatatcgat gttgaagtaa cagaggttta acgattaggc ggttcatcta
    15061 taaatcaaca ttattagcca caccacttaa attgggtatg aactcaattt atgtgatgtg
    15121 gttatttttt tataatcatc atgctctgta tataatttaa atttgaaagt tacatataag
    15181 cgttattagc ttttattgaa aatttcatac atatcgaaca atacatacaa atatttccta
    15241 tacatctaca tctatgacta aataaccctt tattttcaag atgaacttaa actcatctat
    15301 atgctcaata cctctatcat tatcattctt ttgcgataga tgcctctcta atcatatatg
    15361 ttatatttcc caaatgactg ccaggcgact ctataaatat atatttattt agaactttta
    15421 tacataaata gtcttagtaa atcatacgtt ctatgtctga taaaaagttg atacttaagg
    15481 ttgatgtcac acataaaaat gttatttata atataaccat agagtagcaa aggaggtaat
    15541 ataggttatg attatttatc gacagtatca ccatgaaggc gcaccagttt atgaaattat
    15601 aaccaaaacg tttcagcatg tttcaattaa atgtgacgat tcatttagtg atactgaaat
    15661 attcaaattg ctctctttat tacaagacga tatagatcat atgaaagtta gttaaattga
    15721 ctaatcgttc caataatcca gaatttatta aacattgata tagttgttat tattatttta
    15781 gattaatgca tccccgtgca aagaaagaaa cgtaacctat attcatctct gttcccaata
    15841 ataataacaa tacataactg atattagctg tcctttcatc tcctaattaa tatgcgttca
    15901 tgcagctaat atcggttatt tttttgtgct caaagaaatt tttattttaa tctctccatc
    15961 gtcattaatt gctataattt cgtgttcgtg ttgttcaatg aattcgatat tttcatttat
    16021 taaaataggt ttaacgtagt taatttgaca ctggtttgtc tctaatgaca tagccatcag
    16081 catcatttga ctcactaatt gtcctggcac aatctcatta tgtattggat tatcatcatt
    16141 tacgagcgcc aaatattcat ttatccatat ctcattaaac ttcatctcta cttcatcgcc
    16201 ttaataaaag tttgtgtaac cgttatgaca tgttgatcat ttttagttaa tgttaaagcc
    16261 attgtataac gtgtgatatt gcgagtctgt cggcattcaa tatcttctaa atgccctaca
    16321 tatattgtgt ctacttctat cttttgagtt tgattaattg ctgattttgt taaaatcagt
    16381 tcgctatttg catatttttt aaataaatca aattttggcc ataatgacgc acacattaat
    16441 ggtggtactt cttttctata aggttggtta aaacactgat aatatgcagc cacactatcc
    16501 ttagaaaatg acacatttaa tcgctggtta gaatcgagta aataatgctg catttcctag
    16561 acctccccct atccccatag atgcaatcat agtctctttg tcaaacatat aaaataatcg
    16621 agtcactaat tgggcaccgc ttgcaccgta tggatgacct gatgctaatg caccacccca
    16681 tatatttaat tgcgtatttg aaatatttaa agcttgttgg caggcaacta cctgtgcact
    16741 gaacgcttcg ttaatttcaa tgacttcaat attttctatc gttaattgat ttctttttag
    16801 taagttggaa atggctggta ctggaccaat gccaggaaaa ttagaatcaa cacctaccgt
    16861 aacaccatct ttaaataata aaccatgctc gaaacctaat tcgtatgcca tatctttttc
    16921 cataataagc aataaaactg caccatcatt tttcatacaa ctattcgcag cggtaacggt
    16981 cccacctttg atcacgggct taaatcggcc aaagttatct ttcggaatat gtgattttag
    17041 actttcatca gtgttgaata tttctccttt aacggttata ggtaatattt cctgagaaat
    17101 atttccattt tttacatttt ccgctgtcaa ttgatgactt cgataagcaa attcatcttg
    17161 taattctctt gaaacatcat acatcttggc cacattttca gcaccttgaa tcattgatgg
    17221 gtcgctcatt tcaggtgcaa atgatgcacg ctcataaaac tcaggtaatg ctgtttcgta
    17281 cacagaatgc ggtcgtttga ttttccaagg tgctcgactt gtactttcaa caccacctgc
    17341 aatatatacc ttgccagctc cggcttggat catgcgacat gcatattgaa cactttcaag
    17401 tccagaccca cattgccgat cgattgtgac gccaggtatt gaatctttaa gccccgcttc
    17461 aagcaatgct tttcttgcaa tattgccacc attcccaaca acattaccta aaactacatc
    17521 atctatttta gatattacct ctggatactt ctctttaaaa tgttggaata aaggtttaag
    17581 caattgttct ggctctaaat gttttaaagt gccaccatat ttcccaaatg cagttcgttt
    17641 agctgcaact atgactgctt gattcatatt ataattcacc tctcaaatac atcgacatca
    17701 ttttttctct agcaatttta ccacttgcag tgtaatacat cttttctaca tgatgaatca
    17761 tcgatggaat ttcatagcgt ttcactttct tttttaaaaa ttgttttaca tttttatgtg
    17821 taagtgtcac atcaccagaa taaagcaata cgcctatttg accaaaacgc tcatttggaa
    17881 taccgatgat aattgcttca tcaatgctcg aagattgcgt taaaaggcgt tcaacatgtg
    17941 ctggatatat attttgacca ccaataatta acatatcctg ttgacgtccc gttaaatata
    18001 aatactgctc ttttacatag ccattatcat tagtaacaaa ccattcatca ttatttatac
    18061 attgttcact tacatagcca ctaaacatca tattactttt tatacaaata gttcctatac
    18121 cattgtgatc gtgattcgtt gttttcaatt ccacatttgg aaatagcaca cctactgatt
    18181 caactggtgc ttgctgattc aagttatagc tgataaaact ggtttccgat gtaccaaaaa
    18241 attcaatcaa atttatgtca tttgcttgat tttttatctt tttaaaaata gaagaatgca
    18301 gcttatctcc actgctaaaa aatgattgga ttgtatgttc attgttgtaa actaacaata
    18361 atgatttaat catcgttgga acaagaaaca tagcaacttt gtatgatgat attttatgac
    18421 attgattaag taacttttca ggatgaaaag tggtctgtcc tataaaagta cgaccggaac
    18481 ttaaagcaaa caataacgca tataatgtta acgagtgcga tagtggtcca ggggctgcta
    18541 ttgcattttc attttttaac atcaacattt cattaacttc aaaagaagcc aaccatgaat
    18601 cttcatcacg ataatatgct tttggcagtc cagttgtccc tgaagtaaaa ccaatatgta
    18661 ataaattggg gtagtgctgt aattgcgttg aatcaataaa catcggtgag tctatattct
    18721 gcatgagacc tgtatctcca attacatctt taataccata tttttcaatc aattgacgat
    18781 gtatagtact ttgccattta ccttccatca tcataggcac tttatgtaaa cgatgcaaag
    18841 ctaaataaca aataatactt tgcaccggtg atttcatatt aatagcaacg acagggttaa
    18901 gtgaatattt ctgcaaagat tcaactgcgc tagtgatgcg ggcatttagt tgactatatg
    18961 taattgtttc atcatcgata tgtaatgcta tgtcattagg tttattttga gtatgtgttt
    19021 tcaactgttc taaaattacg ttcatcaata tccctcactc aatgtaaact
//
LOCUS       DAKTGS010000041        18733 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.041, whole genome shotgun sequence.
ACCESSION   DAKTGS010000041 DAKTGS010000000
VERSION     DAKTGS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.041"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            704..898
                     /locus_tag="O0286_001210"
     CDS             704..898
                     /locus_tag="O0286_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0169594.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(1131..1982)
                     /locus_tag="O0286_001211"
     CDS             complement(1131..1982)
                     /locus_tag="O0286_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCY0169595.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            2310..3071
                     /gene="sufC"
                     /locus_tag="O0286_001212"
     CDS             2310..3071
                     /gene="sufC"
                     /locus_tag="O0286_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCY0169596.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            3169..4476
                     /gene="sufD"
                     /locus_tag="O0286_001213"
     CDS             3169..4476
                     /gene="sufD"
                     /locus_tag="O0286_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCY0169597.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            4582..5832
                     /locus_tag="O0286_001214"
     CDS             4582..5832
                     /locus_tag="O0286_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0169598.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            5822..6286
                     /locus_tag="O0286_001215"
     CDS             5822..6286
                     /locus_tag="O0286_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCY0169599.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            6437..7834
                     /gene="sufB"
                     /locus_tag="O0286_001216"
     CDS             6437..7834
                     /gene="sufB"
                     /locus_tag="O0286_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCY0169600.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            8021..8158
                     /locus_tag="O0286_001217"
     CDS             8021..8158
                     /locus_tag="O0286_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCY0169601.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(8164..8478)
                     /locus_tag="O0286_001218"
     CDS             complement(8164..8478)
                     /locus_tag="O0286_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169602.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            8843..9883
                     /locus_tag="O0286_001219"
     CDS             8843..9883
                     /locus_tag="O0286_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0169603.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            9897..10964
                     /locus_tag="O0286_001220"
     CDS             9897..10964
                     /locus_tag="O0286_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCY0169604.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            11031..11129
                     /locus_tag="O0286_001221"
     CDS             11031..11129
                     /locus_tag="O0286_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169605.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            11177..12025
                     /locus_tag="O0286_001222"
     CDS             11177..12025
                     /locus_tag="O0286_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCY0169606.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            12038..12865
                     /locus_tag="O0286_001223"
     CDS             12038..12865
                     /locus_tag="O0286_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0169607.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            12892..14211
                     /locus_tag="O0286_001224"
     CDS             12892..14211
                     /locus_tag="O0286_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0169608.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            14295..15212
                     /gene="lipA"
                     /locus_tag="O0286_001225"
     CDS             14295..15212
                     /gene="lipA"
                     /locus_tag="O0286_001225"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCY0169609.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            15342..15725
                     /locus_tag="O0286_001226"
     CDS             15342..15725
                     /locus_tag="O0286_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCY0169610.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(15804..16070)
                     /locus_tag="O0286_001227"
     CDS             complement(15804..16070)
                     /locus_tag="O0286_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCY0169611.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            16168..16602
                     /locus_tag="O0286_001228"
     CDS             16168..16602
                     /locus_tag="O0286_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCY0169612.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            16602..17381
                     /locus_tag="O0286_001229"
     CDS             16602..17381
                     /locus_tag="O0286_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCY0169613.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            17414..18373
                     /locus_tag="O0286_001230"
     CDS             17414..18373
                     /locus_tag="O0286_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCY0169614.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            18627..>18733
                     /locus_tag="O0286_001231"
     CDS             18627..>18733
                     /locus_tag="O0286_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169615.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRIL"
BASE COUNT         6795 a         2678 c         3486 g         5774 t
ORIGIN      
        1 tatagcccgg cagaggccat atatctggct gttggtctca caggagacat cttccttgtc
       61 atcactcata tacatatatc ttgataacat agagttgtta tagtcgctac gccactcata
      121 ctagttactg ggtggttgtt tttatgttat attataaatg atcaaaccac accacctatt
      181 aatttaggag tgtggttgtt ttcaagggca aaaaaagggc ataattttga aacaaagggc
      241 aagggtatga ataataaatt atcttgaaaa ttttataaat gcatatataa caatgttttg
      301 attgcccttg aacttccttg ataatctggc taattattcc tgctaaataa atcatattaa
      361 tggctcagta acgtgatgtt gctgggcttt ttaatttaaa caggtattta tatgatattt
      421 aggaatgaga tgctattacg gataaagtaa aatcctatat gcatgcgtgc tactcattta
      481 ttcttatttt taatttcaca tggctagtga ctaatacatc ataaatcatt agtgattctt
      541 tattatttca gtcccactcc ctcaagttaa tgatgaatct atttcgctag ttataaaatg
      601 acgtcaaatc aatatcaaaa attatttaag taaaatgttt agataatttt tcagtgggta
      661 agtattatat ataacattta attattccga ggaggcattt attatggcag acgaaagtaa
      721 atttgaacaa gcaaaaggta atgttaaaga aacagtaggt aatgttactg ataataaaaa
      781 tttagaaaac gaaggtaaag aagataaagc ttctggtaaa gcgaaagaat tcgttgaaaa
      841 tgcaaaagaa aaagcaactg attttattga taaagtaaaa ggtaacaaag gcgagtaatt
      901 ttacttttta ttcctaatac actgcacatg ccacttagtc ggtataacca tcggctaggt
      961 ggtttttgta ttaaaaaagt ggataccaaa atttattaat aattatttta atgttagaaa
     1021 aaaactaaat aaaaactcgc taatgatatc caataatatg tatacaaaac gagacatata
     1081 ttgcatatga ttaacgagat actgaaaata ttttatcacc cctaaaatga ttattcattt
     1141 tcagcggtaa ttcgacctaa agtcaaactt acaataaaac cgatgataaa tactactaat
     1201 gaaacgaacc acatcacgat attagttggt aaacctggaa atactgcaaa gagggagcca
     1261 acaacaaaac caatgattaa tgcaaaagtc attagtttat gatgtgttag gaaatactgg
     1321 ataattttgc ttgaaataat gaatccagca agcacgccaa atccgactgc aagtaatata
     1381 ggaagacctg caaagttaag tttaacaact tcagatattg ctagcatgac cgtaccatag
     1441 acgccaaata ctaataacat aaatgaccct gaaatacctg ggagtaacat agcactagat
     1501 gcacacatac ctgcaataaa atatttaata ataagactag ttgatagagt aagtgtttct
     1561 ccagcatgtt tatcaccatt attcattaat gtaataacaa ttaagatagc gataccagct
     1621 ataaccatca tgtaatgttt agttgtaaat gacgttttat agttagaaat tttcaataaa
     1681 tatggaacga taccaatgat taatccacca aagaaaaaca tagttggaat atggtgttgg
     1741 cttaataaat aattaaaaag attacttagt gatcccattg ccagtaacat tccaattata
     1801 atggggatta aaaatgtaaa acttggccaa aaacgtcgtg agaatatgcc gctaattgaa
     1861 gcgataaatt gattgtaaat acctaacaat aatgcgatag tcccaccgct aacaccaggt
     1921 accaagtcac tcgttcccat agcaaaacct tttagaatat taatccattt aaattgttgc
     1981 atgaataact cctttcaaac gattggaata aaatcataaa tagcatcata ccatattaca
     2041 aatgtcctag tgaaatgata acatatttta aattcataaa atccattgag aaattatgtg
     2101 cacttattat catttatatt tttaaagaga gcggattaga gttgtacttt agagttatta
     2161 aaaataataa aaagggttta agttgtttat attaaggtat aagtaagtta taattaactg
     2221 aacgcattat tacaaagtct ttttgactac aaattaaaat tattataaac tagttaagaa
     2281 aactttatat tttacggagg gaatataaaa tggcatcaac attagaaatc aaagacctac
     2341 atgtgtctat tgaggataaa gaaatcttaa aaggtgttaa cttgacaatt aacactgatg
     2401 aaatacatgc gattatggga ccaaacggga caggtaaatc aactttatca tctgcaatta
     2461 tgggacaccc aagctatgaa gtaactaaag gagaagtact tttagacggt gtaaatattt
     2521 tagaattaga agttgatgaa agagcaaaag caggattatt cttggcaatg caatatccat
     2581 cagaaattac aggtgttaca aatgctgatt tcatgcgttc agcaatcaat gcgaaacgtg
     2641 aagaaggaca agaaatcaac ttaatgcaat ttattaagaa attagataaa aacatggatt
     2701 ttctagacat agataaagac atggcacaac gttatttaaa tgaaggtttc tcaggtggag
     2761 agaagaaacg taacgaaatc ttacaattaa tgatgttaga acctaagttt gcaatcttag
     2821 atgaaatcga ttcagggtta gacatcgatg cattaaaagt tgtatctaaa ggtattaacc
     2881 aaatgcgtgg ggaaaacttt ggtgcattaa tgattacaca ctatcaacga ttattaaatt
     2941 acattactcc tgataaagta catgtaatgt atgctggtaa agtcgttaaa tctggtggtc
     3001 cagaattagc aaaacgtctt gaagaagaag gatatgaatg ggttaaagaa gagttcggtt
     3061 cagctgaata atcttattaa tacagtatcc atgagatgtt catctatata tgatgaaaat
     3121 gaacatttat acgaaatagt aaatttcatc aagtaggagg aaaaagttat gacaactgat
     3181 attttgaaca tttctgaaga acaacttgtt gattattcta aagcccacaa tgaaccttct
     3241 tggatgacag aattacgtaa aaaagctttg aaattaacag aaactttaga aatgccaaaa
     3301 cctgataaaa caaaattaag aaaatgggat tttgattctt ttaaacaaca cgatgtaaaa
     3361 ggtgatgttt atcaatcttt atcacaatta cctgagtcag taagagaaat tattgacgta
     3421 gatcattcta aaaacttagt aattcaacat aataatacga ttgcgtacac acaagttgat
     3481 gataatgcat cgaaagatgg cgttatcgtt gaaggtttag cagacgctct tatgaaccat
     3541 agtgatttag tacaaaagta ctttatgaaa gatgcagtaa cagtagatga acatcgtatc
     3601 acagcgctac acacggcatt agttaatggt ggcgtatttg tttatgttcc taaaaatgta
     3661 gttgtagaac atccagtaca atacgttgtg ttgcacgacg acgaaaatgc aagcttttat
     3721 aaccatgtta tcatcgttac tgaagaaagc gccgaagtca catatgttga aaattactta
     3781 tcaaatgcat ctggtgaagg aaatcaatta aatattattt ctgaagtgat tgctggtgca
     3841 aattcaaata tcacatatgg ctcagtggac tatatggata aaggctttac aggtcatatc
     3901 attcgacgtg gtattactga agcggatgcc tcaattaatt gggcactagg tttaatgaat
     3961 gagggtagcc aaattattga taatacaaca aatttatttg gtgatcgttc aacaagttca
     4021 cttaaatcag tagttgtagg tacaggcgaa caaaaaatta atctaacatc taaaatcgta
     4081 caatatggta aagaaacaga tggttatatc cttaaacatg gtgttatgaa agaacatgca
     4141 tcgtctgtat ttaatggtat cggctacatt aagcatggtg gaactaaatc aattgctaat
     4201 caggaatcac gtgtattaat gttatctgaa catgctcgtg gtgacgcgaa tcctatttta
     4261 ttaattgatg aagatgatgt acaagctggt catgctgcat cagtaggtcg tgttgatcca
     4321 gatcaacttt actatttaat gagtcgtggt atttctcaaa gagaagcgga acgtcttgtt
     4381 atacatggtt tcttagatcc agtagtacgt gaattaccta tcgaagacgt taaacgtcaa
     4441 ttgagagaag taattgaacg caaagtttct aaataatatt ttgaaaataa aagtttgtaa
     4501 tagatataga ctgtcgatat tggtataaga ctaatacaac gtcagtattt aaatgattag
     4561 gatttttatt taagaaaggt cgtgaatgaa gtggccgaac actcatttga cgttaatgaa
     4621 gtaatcaagg attttccgat attagatcaa aaagtcaatg gcaaacgttt agcatatctt
     4681 gattcaacag cgacaagtca aacgcctgtg caagtgttaa atgttttaga agattactac
     4741 aagcgttata attcaaacgt tcatcgtggt gttcatacat taggatcatt ggcaactgat
     4801 ggttatgaaa atgcccgtga aaccgttcgt cgttttatta atgcgaagta ttttgaagaa
     4861 atcattttta cacgcggaac aactgcgtcg attaaccttg tagcacatag ctatggtgat
     4921 gcaaatgttg aagagggcga tgaaattgtt gtcactgaaa tggaacatca tgccaatatt
     4981 gttccttggc aacagttagc aaagcgtaaa aatgcgacat tgaaatttat accaatgaca
     5041 gctgacggtg aattaaacat cgaggatatt aagcaaacga ttaatgataa aacaaagatc
     5101 gttgctattg cacatatatc taatgtgctc ggtacaatta atgatgttaa aaccattgca
     5161 gaaatagctc atcaacatgg tgcaattatc agtgttgatg gggcgcaagc agcaccacat
     5221 atgaaacttg atatgcaaga aatgaatgct gatttttata gttttagtgg tcataaaatg
     5281 cttggaccaa caggtattgg cgtattattt ggtaaacgtg agttactaca aaaaatggaa
     5341 ccgattgagt tcggtggcga catgattgat tttgtaagta agtatgatgc aacatgggct
     5401 gatttaccta ctaaatttga ggcgggtact ccattaattg ctcaagcaat tgggcttgca
     5461 gaagctattc gctatttaga acgcataggt tttgatgcaa ttcataaata tgaacaagaa
     5521 ttaacgatat atgcttatga gcaaatgtct gcaattgaag gaattgaaat ttatggcccg
     5581 ccaaaggatc gtcgtgcagg tgtaataacg tttaatttac aagatgtaca tccacacgat
     5641 gttgctacag ccgtagatac agaaggtgta gcggttagag ctgggcatca ttgtgcgcaa
     5701 ccgttaatga aatggttaaa tgtgtcttca acagctagag cgagttttta tatatacaac
     5761 acgaaagaag acgttgatca gttaataaat gccttgaaac aaacgaagga gtttttctct
     5821 tatgaatttt aataatctag atcaattata tagatctgtc attatggatc attataaaaa
     5881 tcctagaaat aaaggtgtat tagataacgg gtctatgaca gtagatatga ataacccgac
     5941 atgcggtgac cgtatacgac taacatttga tatagaagac ggcattataa aagatgctaa
     6001 gtttgaaggt gaaggttgtt cgatttcaat ggcaagtgca tcgatgatga cacaagctgt
     6061 taaaggtcat tcacttggag aagcaatgca aatgagccaa gaatttacga aaatgatgct
     6121 tggtgaagac tatgtgatta cagaagaaat gggagatatt gaagcattgc aaggtgtatc
     6181 tcaattccca gctcgtatta aatgtgccac attagcttgg aaagcattgg aaaaaggtac
     6241 tgttgctaaa gaaggtaaag cagaaggtac gactgaagaa gaatagaatg ctgttaatca
     6301 tagataattt tgatattaga catataaaag tataaaaatt tttataagat gtcatgtcat
     6361 tgttataata tggtttacat catgaattaa aaacttacgc acgccgttgt aaatatattt
     6421 ttaaggagtg attgaaatgg ctaaaaaagc acctgatgtt ggggattata aatatggatt
     6481 ccacgacgat gatgtgtcca ttttcagatc agaacgtggt ttaactgaga atatcgttag
     6541 agaaatttct aacatgaaaa atgagccgga atggatgtta gatttccgtc ttaaatcatt
     6601 aaaattgttt tataaaatgc caatgcctca atggggtggc gacttatcag aattgaattt
     6661 cgatgacatt acttactatg taaagccttc agaacaagct gaacgttcat gggatgaagt
     6721 gccagaagaa attaaaagaa ctttcgataa attaggaatt cctgaagctg aacaaaaata
     6781 tttagctggt gtttctgctc aatatgaatc tgaagttgtt taccataata tggaaaaaga
     6841 acttgaagaa aaaggtatta tctttaaaga tacagatagt gctttacaag aaaatgaaga
     6901 attattcaaa aaatactttg cttctgtagt acctgcagca gataacaaat ttgcggcgtt
     6961 aaactcagca gtatggtcag gtggttcgtt catttatgta cctaaaaata tcaaactaga
     7021 tacgccacta caagcttatt tccgtattaa ctctgagaac atgggtcaat ttgaacgtac
     7081 attaatcatt gctgatgaag gtgcttctgt acattacgta gaaggttgta ctgcaccagt
     7141 ttatacaact agttctttac actctgctgt tgtggaaatc attgtgcata aagatgcgca
     7201 cgttcgttat actacgattc aaaactgggc gaacaatgta tacaatttag ttacaaaacg
     7261 tacttttgtt tatgaaaacg gaaatatgga atgggtagat ggtaacttag gttctaagtt
     7321 aacgatgaaa tatccaaact gtgttctttt aggtgaaggt gcaaaaggta gtacattatc
     7381 tattgcattt gctggtaaag gacaagttca agatgccggt gctaaaatga ttcataaagc
     7441 acctaataca tcttctacaa ttgtttctaa atctatttct aaaaatggtg gtaaagttat
     7501 ttatcgcggt attgttcatt ttggacgtaa agcaaaaggt gctcgttcaa atattgaatg
     7561 tgatacatta atcttagata acgaatcaac atcagataca attccatata acgaagtatt
     7621 caacgatcaa atatcattag aacatgaagc caaggtttca aaagtttctg aagaacaatt
     7681 attctatcta atgagtcgtg gtatttctga agaagaagcg acagaaatga ttgttatggg
     7741 attcatcgaa ccatttacaa aagaacttcc aatggaatac gcggtcgaaa tgaaccgttt
     7801 aatcaagttc gaaatggaag gtagtattgg atagctttaa accgcgttgt taagccattc
     7861 ttgacttccg aaaatggcta ttgataccat tttgatactg aatataacaa aaagccacat
     7921 tactgtggct ttttttgttt tataactaaa tcggattgat agataagctt tgtacttatt
     7981 tatatcagtt cgattttttg attggtgtaa aaaataatca ttgatggtgg ataaagcgac
     8041 aacacaaata caacatgatt gtggcattag agtgctggtc tttattaaat taattgaaag
     8101 ctacatcaaa tattctttag ataattcgat attagttcga ttaagattcg ttgtataagt
     8161 gagttaaaat aagaaaacta ttaataatat taagttcact acagatgttg ctaatggacc
     8221 ataagtttta aagacatctt cacttttata accaacaatc gcatctaaaa attgaactaa
     8281 gatcattgca atggatatag ttatcaaaaa tatagcacta tgaatgacta aagaaaaaat
     8341 agctaataaa aataaaggta agcttcgact aagtgcataa tatgcattta tattatggct
     8401 agatgcacat gcttgaattg aataacctaa acttacactg gcactgatta ttgtaaatat
     8461 tgctaaaaca aaatacatgt taatccttct ttctatattt ggatataaac aagtacttgt
     8521 ctaaagttat ttaaaagata attagaataa atttatgaga aactggttgt tatcattata
     8581 atggtttcaa atgattataa ctatgtcata aactgaattt gttgaaattt ttcattatgc
     8641 aaatttatta ataacaaaca gctcgaacta tagcatcatt ttactaatga atgcattaaa
     8701 gtaactatga ctaaaaatgc atattaatta tcattattaa gactattata tataatgaat
     8761 tttaactggt ttattaaacg agaacgtcgg gaattaagta actacaataa aaataagata
     8821 tgacaataag gagactacac gcgtgatcat tgccataatt atattgatat ttatttcgtt
     8881 tttcttttca ggaagcgaga cggcattaac ggctgccaat aaaacaaaat ttaaaactga
     8941 agctgacaaa ggtgataaaa aagcaaaagg cattgtaaag ttacttgaaa aaccaagtga
     9001 gtttattaca acgattctaa ttgggaataa tgtcgcgaat attttattac caacacttgt
     9061 tacaattatg gctttacgtt gggggattag cgttggtatt gcatcagctg ttttaacagt
     9121 tgttatcatt ttgatctccg aagtgattcc caagtctgtc gctgcaacat ttccagataa
     9181 aataacaagg cttgtatatc caattattaa tatttgtgtc attgtgttcc gtcctatcac
     9241 attactttta aataagttga cggacagtat taatcgaagt ttatctaagg gccaacctca
     9301 agaacatcaa ttttcaaaag aagaatttaa aacaatgtta gcaattgctg gacatgaagg
     9361 tgctttaaat gaaattgaga cgagtaggtt ggaaggtgtc attaattttg aaaatttaaa
     9421 agtaaaagat gtagatacaa cacctagaat taatgtgacg gcatttgctt caaatgcgac
     9481 atacgaagaa gtttatgaaa cggttatgaa taagccatac actagatatc cagtgtacga
     9541 gggagatatt gataacatta ttggggtgtt tcattctaaa tatctgttgg cttggagtaa
     9601 taaaaaagaa aatcaaatta caaactattc agctaagcca ttatttgtga atgaacacaa
     9661 taaagctgaa tgggtattac gtaagatgac tatttctaga aaacatttag caattgtgtt
     9721 ggacgaattt ggtggtactg aagcgatagt gtcacatgaa gacttaattg aagaattatt
     9781 aggtatggaa attgaagatg agatggataa aaaggaaaaa gaaaaacttt ctcaacagca
     9841 aattcaattt caacaacgga aaaatcgcaa cgtatctata taaggagcga acagctatgt
     9901 ggaataagaa tcgacttact caaatgttaa gtattgaata tccaattata caagcaggta
     9961 tggcaggaag tacgacaccg aaattagttg catcagtaag taacagtggt gggttaggca
    10021 caataggcgc aggttacttt aatacgcagc aattggaaga tgaaatagat tatgtacgcc
    10081 aattaacgtc aaattctttt ggcgtaaatg tctttgtacc aagtcaacaa tcatatacca
    10141 gtagtcaaat tgaaaatatg aatgcatggt taaaacctta tcgacgcgca ttacatttag
    10201 aagagccggt tgtaaaaatt accgaagaac aacaatttaa gtgtcatatt gatacgataa
    10261 ttaaaaagca agtgcctgta tgttgtttta cttttggaat tccaagcgaa cagattataa
    10321 gcaggttgaa agcagcgaat gtcaaactta taggtacagc aacaagtgtt gatgaagcta
    10381 ttgcgaatga aaaagcgggt atggatgcta tcgttgctca aggtagtgaa gcaggtggac
    10441 atcgtggttc atttttaaaa cctaaaaatc aattacctat ggttggaaca atatctttag
    10501 tgccacaaat tgtagatgtc gtttcaattc cggtcattgc cgctggtgga attatggatg
    10561 gtagaggagt tttggcaagt attgtcttag gtgcagaagg ggtacaaatg ggcaccgcat
    10621 ttttaacatc acaagacagt aatgcatcag aactactgcg agatgcaatt ataaatagta
    10681 aagaaacaga tacagtcatt acaaaagcgt ttagtggaaa gcttgcacgc ggtatcaaca
    10741 ataggtttat cgaagaaatg tcccaatacg aaggcgatat cccagattat ccaatacaaa
    10801 atgagctaac aagtagcata agaaaagccg cagcaaacat cggcgacaaa gagttaatac
    10861 atatgtggag tggacaaagc ccgcgactag caacaacgca tcccgccaac accatcatgt
    10921 ccaatataat caatcaaatt aatcaaatca tgcaatataa ataatcgacc gcaatccaca
    10981 aaagcacaag cacccccaaa cattatttta gtgcttgcca tttttgtgga ttgcgtttct
    11041 attttacaaa tttaatcaaa cgaaaacatc aagctgaaga tcgccgaaag attttaatca
    11101 agcaaaaaca tcaaactaaa gttcgctgaa atgattatga taaaagttat atggtatgat
    11161 gacattggtg atatatatga taaacatcgg attaacaggt tggggtgatc actattcatt
    11221 atatgaagat ttagaacgcc aaaccgataa acttaaaaca tatgctggac attttccggt
    11281 tgtcgaatta gatgcgacat actatgcgat acaaccggaa agaaatatat tgaaatggat
    11341 aaaagaaacg cctgatacat ttgaatttgt ggtcaaaatt catcaagcac tcacattgca
    11401 tgcagactac aaaacatttg cagatacaag gcaagaacta tttgatcaat ttaagaatat
    11461 gttagagccc ttacatacac agaaaaaatt agcaatggta ttggttcaat ttccgccatg
    11521 gtttgactgc aatgcacaaa atatcaaata tattttgtat gtaagacagc aattacaagc
    11581 atttccaatg tgtgtagaat ttaggcatca atcatggttt agtgatgcat ttaaagaaca
    11641 aacattggca tttttaacag aacatcaaat cattcatgca gtagttgatg aaccacaagt
    11701 gaaagatggc agtgtacctt tagtcaatcg aatcacaaat gaaattgcgt ttgtacgtta
    11761 tcatggacgt aatcattacg gttggactaa gaaagatatg tcagatcaag aatggcgcga
    11821 tgtacgctat ttatatgatt ataatgagca agaattaata gacttggcac aaaaggcaca
    11881 aatattagca caaaaagcta agaaagttta cgtcatattt aacaataatt ctggtggtca
    11941 tgcagcaaat aatgccaaaa catatcagcg attattgaat atagaatatg aagggttagc
    12001 accacaacaa ttaaaattat tttaagaggc gacgactatg ttattaacaa ttacattatt
    12061 agttttaatc ggaggtttgt cagcgattat agggtctatc gtaggcattg gaggcggtat
    12121 tattatcgtt ccaacaatgg tttacctcgg tgttgaacat ggattactac ataatattac
    12181 aacacaagta gcgataggga cgtcttcagt cattctaatt gtgacaggac tttcttcatc
    12241 acttggatat ttaaaaacaa aacaagttga tattaaaaat ggttccatct ttttatttgg
    12301 actattacca ggttcattgc ttgggtcctt cattagtaga tatttaacat ttgagtcatt
    12361 taatttatat tttggtatct ttttaatttt cgtagccatt ttattaatgg taagaaataa
    12421 gattaaaccg tttaaaattt tcgataaacc caagtatgaa aagacttatg tagacgctaa
    12481 aggtaaaaca tatcattata gtgttccacc attgtttgct tttattacaa cgtttttaat
    12541 tggtatattg acaggtttat ttggtattgg aggtggcgca ctaatgacgc cactaatgct
    12601 tattgtattt agatttccac ctcatgtagc tgttggaaca agtatgatga tgattttctt
    12661 ttcaagtgtc atgagttcta tagggcacat tgctcaaggt cacgtagctt ggggttatgc
    12721 aatcatttta attatttcta gttattttgg tgcgaaaatc ggtgtcaaag tgaatcaatc
    12781 aattaagtca gatacggtag taacattatt gagaacagta atgttgttaa tgggtatata
    12841 tttaattatt cgtgcgttga tttaatacaa ctttaaaagg aggacgtcaa tttgaggctt
    12901 acaatttatc atacgaacga tattcatagt catttacatg aatacgaacg cattaaagca
    12961 tatatggcag aacatcggcc acgacttaat catccttctt tatatgttga tctaggtgat
    13021 catgtagatt tatccgcacc tataactgaa gcaactttag gtaaaaagaa tgtggcatta
    13081 ctaaatgaag caaaatgtga tgttgcaaca atcggtaata atgaagggat gaccatttca
    13141 cacgaagctt taaatcacct ttacgacgaa gcaaaattta tagtgacatg tagcaatgtt
    13201 atagatgaat caggtcattt accaaataat atcgtttctt cttatattaa ggacatagac
    13261 ggtgtgaaaa tactattcgt tgcagcgaca gcacctttta ccccatttta tcgtgcacta
    13321 aattggattg ttaccgatcc acttgaatct ataaaagaag aaattgaact tcaacgaggt
    13381 aaatttgatg tattaatcgt gctaagtcat tgtggcattt tcttcgatga aacattatgc
    13441 caagaattgc ctgaaattga tgtcattttt ggtagtcata cgcatcatta ttttgaacat
    13501 ggtgaaatca ataatggtgt actgatggcg gcagctggaa agtatggtaa ttatcttgga
    13561 gaggttaatt taacttttga ggcacataaa gtagtacata aaactgcaaa gattattcct
    13621 ttagaaacat tacctgaagt tgaaacttca tttgaagaag aaggaaaaac gttaatgtcc
    13681 aattcagtaa ttcaacatcc agtagtgctt aagcgtagta tgaatcacat aactgaagct
    13741 gcatacttat tagctcaaag tgtttgtgag tatacacatg cacaatgtgc catcatcaat
    13801 gctggcttac tcgttaaaga tattgtaaaa gatgaagtga cagaatatga cattcatcaa
    13861 atgttaccgc atccgattaa tatggtaagg gttagacttt ttggtgtgaa attaaaagag
    13921 attatagcta aaagtaataa acaagaatat atgtatgaac atgcacaagg tttgggtttc
    13981 agagggaata tatttggagg atatattctt tataatttag ggtacattca ttctacaggg
    14041 cgttactatc tgaatggaga agaaatcgaa gacgacaaag aatatgtact aggtacgata
    14101 gatatgtata cgttcggtcg ttatttccca acattgaaag aattaccaaa agagtattta
    14161 atgccagagt ttttaagaga tatatttaaa gaaaaattat tggaatatta aaaagtaaga
    14221 ttattggatt ttcatttgtc atgaatttcg atataatgtt taaagataca cttaacagga
    14281 gggtatgtgt tgttatggcg acaaaaaacg aggaaatatt acgtaaaccg gattggttga
    14341 aaataaaatt aaataccaac gaaaactata caggacttaa gaagatgatg agggaaaaaa
    14401 atcttaatac tgtatgtgaa gaagctaaat gtcctaatat acatgaatgt tggggtgcac
    14461 gtcgtacagc gacatttatg attttaggtg ccgtatgtac aagagcttgt cgtttttgtg
    14521 cggttaagac aggtttacct aatgaacttg atttaaatga gcctgaacgt gtagctgaat
    14581 cagttgaatt aatgaatttg aaacacgttg ttatcactgc tgttgcgcgt gatgatttaa
    14641 gagatgctgg ttcaaatgtt tatgctgaga cagtacgtaa agttagagaa agaaatccat
    14701 ttacaacgat tgaaatttta ccatcagata tgggcgggga ctatgatgcg ttagaaacat
    14761 taatggcgtc aagacctgac attttaaacc ataatattga aactgttcgt cgcttaacac
    14821 cgagagttcg tgcgcgtgcg acttacgaca gaacattaga gtttttacgt cgttcaaaag
    14881 aattacaacc ggatatccca actaaatcaa gtattatggt tggattaggt gaaactatag
    14941 aagaaattta tgaaacgatg gatgatttac gtgcgaatga tgtagatatt ttaacgattg
    15001 gtcaatattt acaaccttca cgtaaacatt taaaggttca aaaatattac acgcctttag
    15061 agtttggtaa attaagaaaa gtggcaatgg ataaagggtt taaacattgc caagctggac
    15121 ctttagtacg tagttcttat catgcggatg agcaagtaaa tgaagctgct aaagaaaagc
    15181 aacgccaagg tgaggcacag ttaaatagtt aatatttaac cattaataag gcataaaggc
    15241 ttagtttgta caaaacgaac gtgtcataga agtaatcatt atttttatga aacactaaaa
    15301 gtataaactg agcctttcat tgtcatagat aggtgaagaa tttgataaaa gtagatcaac
    15361 attactttga attaatagaa aattatcgcg aatgttttaa tgaagaacaa tttattgcta
    15421 ggtattcaga tattttagat aaatatgatt acatagttgg tgactatggt tacgatcaat
    15481 tacgattaaa aggtttttac aaagattcta ataaaaaagc agagatgagt aaacgttttt
    15541 caaatattca agattacata tttgaatatt gtaactttgg ttgtccttac tttgtattaa
    15601 gacatttgtc taaacaagag gttaaaaagt taatcgaaga agttcatccg tctgatgtga
    15661 tagatgacga caataaactt caagatgtga agattaagcc aaccattcaa gatactgaac
    15721 attaataaaa cccttagcta gattgaaaat gggaatcatg caattcaagc atggacctgt
    15781 aatctagtta ggggttttta tctttaatga atgacttcat ttaaatactc agtaatttca
    15841 tcgccttctt cagcatttac acctaaaata tgagcgatat agccttcttc ttttaaatca
    15901 tcagtaccga taataccgaa tttatttgtt tgcatattaa gtacgagtgt cttaccataa
    15961 tgtctatttg tatggactaa catcaaatca tatcgactat gctcgccaac aaaaccaaca
    16021 aactgaactt gactctcttc gttgtcatca tataaataca tatcaatcat tttgtagcga
    16081 ctccttttaa aagtagtaaa gttagtataa cgacaaatga agtatactgc aaaattatga
    16141 taatatataa gtgagaggtg acaaggaatg tattttgtag acaaagataa actaactcag
    16201 aaattagcct atttacaagc attaactgat gattatcatg agagcaagca caatcattat
    16261 gcatttgaac gcattgctca aatgttgata gaatcatcgg tagatatagg gaatatgatt
    16321 atcgatgcat ttattttaag ggatcctggt aattataaag atgtgattga tatattagaa
    16381 ctagaaaatg ttattactaa agaaacacag caggcgatta ataaaactgt cggtattcgt
    16441 aaacaattta catatgatta cacagcctta gatattaaga ttatcatgcc aatgtttgat
    16501 gacgcattac cttattacaa acaatttatt acagaagtaa cgacattttt acatcaagaa
    16561 aatgtaccag taacagcttt tggtaaagga gaaaatcaat aatgaaacag tataaagcgt
    16621 atttaatcga tttagatggc acaatgtata tgggaacaga tgagattgat ggagcaaaac
    16681 aattcatcga ttatttaaat gtaaaaggca ttcctcattt atacgtaact aataattcaa
    16741 caaaaacacc tgagcaagta actgaaaaat tacgtgaaat gcacattgat gctaaaccag
    16801 aagaggttgt aacgtcagcg ttagccactg ctgattatat ttcagaacaa tcaccaggag
    16861 catcagtata tatgttaggt gggagtggtt taaatactgc gttaaccgaa gcgggacttg
    16921 tcattaaaaa tgacgagcat gttgattatg tagttattgg acttgacgaa caagttacat
    16981 atgaaaagct tgcgattgca acgttaggtg taagaaatgg tgcaacattt atttctacaa
    17041 atcctgatgt atcaattcct aaagagcgtg gtttattacc tggtaatggt gctattacaa
    17101 gtgttgtaag tgtatcgaca ggtgtatcgc cacaatttat tggtaaacca gaaccgatta
    17161 ttatggttaa agcattagaa attttaggat tagataaatc cgaagttgct atggtaggcg
    17221 atttgtacga taccgatatt atgtctggta ttaacgtagg tatggatacg attcatgtac
    17281 aaacaggtgt atctacgtta gaagatgtgc aaaataaaaa tgtgccacca acgtattctt
    17341 ttaaagattt aaatgaagca atagctgaat tagaaaaata gatatagtca ttttataaag
    17401 taggtgaatt gatttggtaa aaatagttgt ttcgaggaaa attccagata aattttatca
    17461 acaattaagt aaacttggtg acgttgttat gtggcaaaaa tcattagtgc ctatgcctaa
    17521 agatcaattt gtgacagcac ttcgtgacgc agatgcttgt tttattacat taagtgaaca
    17581 gatcgatgca gaaattttag cgcaatcacc aaatttaaaa gtaattgcga atatggctgt
    17641 aggatatgac aacatcgatg ttgaaagtgc aacagcgaat aacgtggttg tcacgaatac
    17701 accaaatgta cttactgaaa caactgcaga attaggattt acattaatgc ttgctatagc
    17761 acgccgtatt gtagaagctg aaaaatatgt agaagcagat gcatggcaaa gctggggtcc
    17821 ttatttattg tcaggtaaag atgtcttcaa ttcaactatt ggaatatatg gtatgggaga
    17881 tattggtaaa gcttttgcaa gaaggttgca agggtttaat actaatattc tttatcataa
    17941 tcgatcaaga cataaagatg cagaggcgga ctttaatgca acatatgttt cttttgaaac
    18001 gttgttagca gaaagtgatt ttatcatctg tacagcgcca cttacaaaag aaacacatca
    18061 taaatttaat gctgaagcat ttgaacaaat gaaaaatgat gcaattttta ttaatatcgg
    18121 tagaggacaa attgtagatg aaacagcatt aatcgatgca ctagacaata aagaaatttt
    18181 agcatgtggt ttagatgtat tagcaaatga accgattgat catacacatc cattaatggg
    18241 acgtgataat gttctgatta caccacacat tggtagcgca tcagtaacaa cacgggacaa
    18301 tatgattcaa ttatgtatta ataatataga agcggttatg acaaatcagg taccacatac
    18361 tccagtaaat tgaaaataag tatggttttt aatcattggt aaaacaagca aagcaattgt
    18421 taaatatgtt atgcttgact tgcgaatgta tatatttgcg ctgatgataa ttcaataaca
    18481 ttataaactc gtcatattaa tgtgatgagt ttatttgatt ttgacgtata taacaaaatt
    18541 gttatataga ctgtaattgt ttattgttta aataaccttt aaaatgaatg atgttctcaa
    18601 caaagtgtat catttacaat aattgagtga tgacaaccaa ttactatgtt gaatctataa
    18661 aattaaaatt gaatttcatt atgaatatcg atataatgaa ttgtaaaaaa caaattttaa
    18721 aacgtatttt ata
//
LOCUS       DAKTGS010000042        18669 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.042, whole genome shotgun sequence.
ACCESSION   DAKTGS010000042 DAKTGS010000000
VERSION     DAKTGS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18669
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.042"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            83..1429
                     /locus_tag="O0286_001232"
     CDS             83..1429
                     /locus_tag="O0286_001232"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCY0169616.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            1451..2266
                     /gene="ccsA"
                     /locus_tag="O0286_001233"
     CDS             1451..2266
                     /gene="ccsA"
                     /locus_tag="O0286_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCY0169617.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            2308..3234
                     /gene="hemC"
                     /locus_tag="O0286_001234"
     CDS             2308..3234
                     /gene="hemC"
                     /locus_tag="O0286_001234"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCY0169618.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            3256..3924
                     /locus_tag="O0286_001235"
     CDS             3256..3924
                     /locus_tag="O0286_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCY0169619.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            3927..4901
                     /gene="hemB"
                     /locus_tag="O0286_001236"
     CDS             3927..4901
                     /gene="hemB"
                     /locus_tag="O0286_001236"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCY0169620.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            4949..6235
                     /gene="hemL"
                     /locus_tag="O0286_001237"
     CDS             4949..6235
                     /gene="hemL"
                     /locus_tag="O0286_001237"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0169621.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            6296..6397
                     /locus_tag="O0286_001238"
     CDS             6296..6397
                     /locus_tag="O0286_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169622.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            6431..7504
                     /locus_tag="O0286_001239"
     CDS             6431..7504
                     /locus_tag="O0286_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCY0169623.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(7727..8287)
                     /locus_tag="O0286_001240"
     CDS             complement(7727..8287)
                     /locus_tag="O0286_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCY0169624.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     misc_binding    8345..8646
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8690..11320
                     /locus_tag="O0286_001241"
     CDS             8690..11320
                     /locus_tag="O0286_001241"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCY0169625.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            11333..12604
                     /locus_tag="O0286_001242"
     CDS             11333..12604
                     /locus_tag="O0286_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCY0169626.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            12874..13581
                     /locus_tag="O0286_001243"
     CDS             12874..13581
                     /locus_tag="O0286_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCY0169627.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            13578..14264
                     /gene="radC"
                     /locus_tag="O0286_001244"
     CDS             13578..14264
                     /gene="radC"
                     /locus_tag="O0286_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCY0169628.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            14332..14616
                     /locus_tag="O0286_001245"
     CDS             14332..14616
                     /locus_tag="O0286_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169629.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(14733..14930)
                     /locus_tag="O0286_001246"
     CDS             complement(14733..14930)
                     /locus_tag="O0286_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169630.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            15090..15563
                     /locus_tag="O0286_001247"
     CDS             15090..15563
                     /locus_tag="O0286_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCY0169631.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            15637..15756
                     /locus_tag="O0286_001248"
     CDS             15637..15756
                     /locus_tag="O0286_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169632.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            15956..16798
                     /gene="mreC"
                     /locus_tag="O0286_001249"
     CDS             15956..16798
                     /gene="mreC"
                     /locus_tag="O0286_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCY0169633.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            16798..17328
                     /gene="mreD"
                     /locus_tag="O0286_001250"
     CDS             16798..17328
                     /gene="mreD"
                     /locus_tag="O0286_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCY0169634.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    17355..17482
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            17495..17803
                     /gene="rplU"
                     /locus_tag="O0286_001251"
     CDS             17495..17803
                     /gene="rplU"
                     /locus_tag="O0286_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCY0169635.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            17809..18129
                     /locus_tag="O0286_001252"
     CDS             17809..18129
                     /locus_tag="O0286_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCY0169636.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            18141..18425
                     /gene="rpmA"
                     /locus_tag="O0286_001253"
     CDS             18141..18425
                     /gene="rpmA"
                     /locus_tag="O0286_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCY0169637.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
BASE COUNT         6515 a         2539 c         3506 g         6109 t
ORIGIN      
        1 aatttattta agcatcgtac ttaattggat tttaaattat gttataatat ttgtattgtt
       61 agtatatatg ggggcgtttc aaatgcattt tattgcaatt agtataaatc atcgcacagc
      121 tgatgttgca ctaagagagc aagttgcttt tagagatgat gccttacgaa ttgcccatga
      181 agatttatat gaaactaaat ctattttaga aaatgtcata ttatcaacat gtaatcgaac
      241 tgaagtatat gctgttgttg atcaaattca cacaggtcgt tactatattc aacgatttct
      301 agctcgtgca tttggatttg aagtagatga tattaaagca atgtcagaag taaaagtggg
      361 ggacgaagca gtagaacatt tattgcgtgt cacttctggt ttagattcaa tcgtacttgg
      421 agaaactcaa attttaggtc aaataagaga tgcatttttc ttagcgcaaa gcacaggtac
      481 gacaggaaca atttttaatc atctatttaa acaggcaatt acttttgcaa aaagagcaca
      541 taatgaaaca gatatagctg ataatgctgt aagtgtgtct tatgctgcgg tcgagttggc
      601 gaaaaaagta tttggcaaat tgaaaagtaa gcaagctatc attattggtg caggggaaat
      661 gagtgaatta tcactattaa atcttcttgg ttctggaatt actgatatta cagtagtaaa
      721 tagaacaatt gaaaatgcta tgaaattagc agcaaagcat caagtgaaat atgatgaact
      781 atcatcatta ccaaatttac ttgaaagtgc agatattgtg attagttcaa cgagtgcaca
      841 atcttatatc attacaaatg aaatgataga aagaattgca gaaaatagaa agcaagattc
      901 actagtattg attgatattg cagttcctcg agatattgaa cctggtatta gtgccatcac
      961 aaacatcttt aattatgatg ttgatgactt aaaaggttta gttgatgcaa acttacgtga
     1021 gcgacaatta gcggctgcaa caatttcgga acaaattcct gcagaaatac atgcacacaa
     1081 tgagtggatt agtatgttgg gtgttgttcc agtgattaga gctttacgtg aaaaagctat
     1141 ggcaattcaa gcagaaacga tggacagtat tgatcgtaaa ttgccagggt taagcgaaag
     1201 agaacgtaaa attatatcta aacatacaaa aagtatcatc aatcaaatgt tgaaagatcc
     1261 tattaaacag gccaaagaat taagtagtga taagaaaagt aatgaaaaat tagagctatt
     1321 tcaaaatata tttgatatcg aagctgaatg tcctcatgag caagcgaagc aacaaaaaga
     1381 aagcaaggtc aaggaaattt cagcgcgacg tatctttagt tttgaataag catattaata
     1441 tggtgataat atgcaagaaa acctgtttat tcgattcaat gaaattatat tattaatata
     1501 cttaatcagt atcatttgct atttttatga ttttgtacaa aaaagtcata agattagaag
     1561 tttaggcata tatttattgg ggattgtttg ggttttacaa acaatctctt tatctatttt
     1621 tattatacaa actagacata ttccattagg gtctatttca gatgtattct atactttaag
     1681 ttggctgatt atttcaatct ctttaattct taaccttatc aaagttttga atttctctgt
     1741 gtttttcttg aatttaatag ggttaacact tttagggatg aatacatttc aaccgacaca
     1801 ttatactaat aaggttcaga aaattgctgt tgtagacgag ttactgttag tccatatcgg
     1861 tctagctgtg ttaagttatg tcttctttgc cttagctttt gtcaatgcat tactgtatat
     1921 catgcaatac cgtaatttga aggaaaaacg ttttgatcaa aaatacttta gaattggtag
     1981 cgttgctaca cttgaatcaa ttgtgtttta ctcaacgtta agtgggtgga taattctcat
     2041 atttagtatt attttaggta cacagtgggg tgtaatttca gttggtgaac gtatattcat
     2101 tgacccgaag gtaatactct cttcaattat tacagtatta tatggaagtt atattttact
     2161 tagaatcaac aaatggttga attcaagata tttaatatac tataacatta ttttattttg
     2221 cttaaatatg attaatttat tttttgcaac acattttgtg aattgagaaa ataatctaga
     2281 gcaatccgtt cctaggagga attgaacatg cgtaaattag tcgttggctc cagaagaagt
     2341 aaattagctt taacacaaag ccagcaattt attgataaat taaaagctgt cgagccaaat
     2401 ctagaaattg aaattaaaga aattgtcacg aaaggcgatc gtatagtaga taaacaattg
     2461 tctaaagtcg gaggcaaagg cttatttgtt aaagaaatac aacatgaact ttttgaaaaa
     2521 aatatcgata tggcaataca ctcgcttaaa gacgtaccaa gtgtaattcc ggaaggttta
     2581 acattaggtt gtatccctga tagagaatta ccttttgatg cgtatatttc taaaacacat
     2641 acaccactat cccaattgcc agaaggcagt attattggta ctagttcatt acgtcgtggt
     2701 gcacaaatat tatctaagta tcctaattta gagattaaat ggattagagg taatatagat
     2761 acacgattag aaaagttaca aactgaagat tatgatgcga ttattttagc tgcagctggt
     2821 ttaagaagaa tgggctggtc agatgatatt gtaacatctt atcttgatag agatacattg
     2881 ttacctgcaa tcggacaagg tgctttaggg atagaatgtc gtagtgacga tgaagaacta
     2941 ttaacattat taagcaaagt acataatgat gaggttgcaa aatgtgtgac tgctgaacga
     3001 acgtttttag cagaaatgga tggtagttgt caggtgccaa ttgcaggata tgctacaatc
     3061 tcagatcaaa acgaaatcga atttacaggt ttaattatga ccccagatgg taaagaacga
     3121 tttgaatata caatgaacgg aacagatccg gttgagttag gcaaaacagt gagtaacaaa
     3181 ttaaaagagc aaggtgctta tgaaattata aaacgcttaa atgaacaaca ttaaataatg
     3241 gagtgacgtt taaatatgaa gccagttgta gttatgacac aaacaaatga catgcaaagt
     3301 gatttagtat caattattca caaacccttt attgatataa agccactaaa ttttgatatt
     3361 catttgttaa atcaacgcta tgactggctt attttttcat ctaaaaatgc tgtgaaattc
     3421 ttttataaat atctaaaagg aattaatgtt gataacattg ctgtgatagg aagtaagaca
     3481 gcgcaatatt gtgaatcact tggcattcga gttgatttta tgccaaacga cttttctcaa
     3541 gaaggatttt taaaatcatt taatcaaact aaccaaaaaa tacttttgcc ttcgagtgaa
     3601 ttggcgagac cattgttatt agcagcgtta tctaaagata atgaagttgt taaaatagat
     3661 ttatatactt cagtgcctaa caaacaaaat atacaagatg ttaaagaaat gatagaacat
     3721 caacaaatcg atgcattaac attttcaagt tcgtcggcag tacgttatta ttttaatgaa
     3781 ggatttgtac caaaattcaa gtcgtatttt gctattggag aacaaacagc acggaccatt
     3841 aaatcatatc aacaaccagt aacaattgca gaaattcaaa cactcgaatc actaattgaa
     3901 aagattttag aaagtagggg ctaaaaatga aatttgatag acatagaaga ttgagatcat
     3961 cagcgacaat gagagatatg gttagagaga atcatgtaag aaaagaagat ttaatatatc
     4021 caatttttgt agttgaaaaa gacgatgtga aaaaagaaat taagtcattg ccaggtgtat
     4081 accaaatcag tttgaattta cttgaaagtg aattaaaaga agcttatgac ttaggcatac
     4141 gtgccattat gtttttcggt gttccaaact caaaagatga tataggtact ggtgcataca
     4201 ttcacgatgg tgttattcaa caggcaacac gtattgctaa aaaaatgtat gatgacttat
     4261 taattgttgc agacacttgt ttatgtgaat atactgatca tggtcattgt ggcgtgattg
     4321 atgaccatac acatgacgtt gacaatgata aatcattgcc actacttgtt aaaacagcaa
     4381 tttctcaagt ggaagctggt gctgatatta ttgcgccaag taatatgatg gatggttttg
     4441 ttgctgaaat tcgtcgtgga ttagatgaag ccggctatta caatattcct ataatgagtt
     4501 atggtgtcaa gtatgcatca agtttctttg gaccttttag agatgcagca gattcagcgc
     4561 catcatttgg ggatagaaaa acgtatcaga tggaccctgc taaccgtttg gaagcacttc
     4621 gtgaattaga aagtgatctt aaagaagggt gcgacatgat gattgttaaa cctgctctaa
     4681 gttatttaga tatagttcga gatgttaaaa atcatacgaa tgttccagtt gttgcatata
     4741 atgtgagtgg agaatatagt atgactaaag cagcggcaca aaatggttgg atagatgaag
     4801 aacgtgtcgt tatggaacaa atggtttcaa tgaaacgtgc aggtgctgat atgattatta
     4861 cgtattttgc aaaggacatt tgtcgctatt tagataaata aggttttata tttatgattt
     4921 tccataaact gtaggaggaa tttactttat gagatatacg aaatcagaag aagcaatgaa
     4981 ggttgctgaa actttaatgc ctggtggtgt aaatagtcca gtacgcgcat ttaaatcagt
     5041 agatacacca gcaattttta tggatcacgg taaaggttca aaaatttatg atatcgatgg
     5101 taacgagtat atcgactatg tactaagttg gggaccactt attttaggac atagagaccc
     5161 tcaagttatt agtcatttac atgaagcaat tgataaaggt acaagttttg gtgcatcaac
     5221 attacttgaa aataaattgg cgcagctcgt tattgaccga gtaccttcaa tagaaaaagt
     5281 gcgtatggtg tcatctggta cagaagctac attggatact ttaagattag cacgtggtta
     5341 tactggcaga aataaaattg tgaaatttga aggttgctat catggtcata gtgattcgtt
     5401 attaatcaaa gctggttctg gggtggcaac attaggattg ccggattctc ctggtgtgcc
     5461 tgaaggtatt gctaaaaata caattacagt tccatacaat gatttagatg cacttaaaat
     5521 cgctttcgaa aaatttggaa acgatattgc tggtgtaatc gtagaacctg ttgctggtaa
     5581 tatgggtgtc gtaccgccga ttgaaggttt tttacaggga ttaagagata ttacgactga
     5641 atacggcgca ttgctaattt tcgatgaagt aatgactggt ttcagagtcg gttatcattg
     5701 tgcacaaggt tactttggtg tgacaccaga tttaacttgc ttaggaaaag ttatcggtgg
     5761 aggactacct gtaggtgcat ttggtggtaa aaaagaaatc atggatcata tagcaccatt
     5821 aggaaatatt tatcaagcgg gtacgttatc aggaaatcct cttgcaatga caagtggtta
     5881 tgaaacgtta agccaattaa cgccagagac atatgagtat tttaatatgt taggcgatat
     5941 acttgaagac ggtttaaaac gtgtatttgc taaacacaat gtaccaataa ctgtaaatag
     6001 agcaggttca atgattggtt atttcttaaa tgaaggacct gtaactaatt ttgaacaagc
     6061 gaataaaagt gatttgaaat tatttgcaga aatgtatcga gaaatggcaa aagaaggtgt
     6121 gtttttacca ccatctcaat ttgaaggtac attcttatct acggcacaca cgaaagaaga
     6181 tattgaaaaa acgattcaag catttgatac ggctttaagt cgtattgtaa aataaatata
     6241 cggacaaatt gagagcctga actttgttca ggctcttttt aaatgtatat aaggcatggg
     6301 cggcgacttg atagtgaaag tccatatatg ctttgtagtc aaaactgcta gcggatattg
     6361 ttatcttaac aaacgtgaag ctcaagcagc aatttagtca ttttattttt tattgaaaga
     6421 agtggaaaac atggcaatga tatatagaaa taatttcatt gtgttcgttt tatcattttt
     6481 tattagtatt ctactgtatt catcgcacgt attacttcca tttatgtttg gtcctattat
     6541 cgcatcaatc atttgtgtaa aagttttcaa acttgatatt aaatggccat tcttacttag
     6601 tgaattaggt attgtactat taggtgtgca aatcggatca acgtttacga aaaatgtcgt
     6661 tatggatatt aaaaacaatt ggctttcgat tattgttgta tctatttcga tattattaat
     6721 tgcaatagta atggcatttg tttttaaaaa aattgcacgt attaatacag aaacagctat
     6781 tttaagtgtt ataccaggag cactaacaca aatgctggtc atggctgaac aagacaaacg
     6841 tgctaatttg ttagttgtta gcttaacgca aacatcacga attatatttg ttgttgtttt
     6901 agtaccgttc atttcatatt tttttcatga tggtaacatg catgcgaatg gaaagttaac
     6961 aaaagtcttg cctttatcac aagtattaaa catagggcaa atagttattt tagcgatagc
     7021 tatctttata gtttatctaa ttatgtctaa aataaagttt ccaacatttc aattattagc
     7081 accactcatt gtattaattg tttggaattt ttctacaggt ttaacattta cactagatca
     7141 ttggttgttg aacatggcac aactaatata tatgattaga attggagttc aaattgcgca
     7201 tttattgtca gatttaaaag gtagactagc aatcgcaatt acaattcaaa atattatgtt
     7261 gataattggt gcgctaatca tggtttatat catacatttc tttgataata atcctattaa
     7321 tgaattgttt cttggtgcag caccaggagg ggttaatcag atagttttag ttgcattagc
     7381 tactggtgcc gatgttgcta tgattacgag ttatcatatt ttccgaatct ttttcatcct
     7441 atttattatt gcaccaggaa ttaattactt tttaaaatat cgttcgaata agcgtgaaga
     7501 ttagaaacta tcatccatct aagttgaatt atgcttagga tagaattcat tattaaggtt
     7561 taagcaaagg ttgtacatct atttttagat taagatatct aatattagca gagcattaac
     7621 ttggaacttt aacttatgat ttgaataaat tttcacatta aaaaacgaga cagtctatta
     7681 gttaaaaata gatgatgtct cgtttacttt atgatgctat agcaaattaa ttatgtttag
     7741 gctttgatgg acaatcttgt aaatgtgcat catataaacc ggctgcttct aaaaacgaaa
     7801 atactgttac tggacctaaa aatttaaacc cgtattgttt taaatcttta gatagttgtg
     7861 ttgcagtatc atcaactgtg atacgatcag aagcatgttc atactgcaaa tctttaggct
     7921 taccatttac atatgaccat aaaaatttac taaaactacc atatgcttgt tcaattttta
     7981 aatacccttg agcttgatta acaattgctt ctaatttttt acgatgatga acgatatttg
     8041 gaaaagtcat taagcggtcg atatcttgag cggtcatttg tgctaccttt tctggttcga
     8101 aatcataaaa tgcttcttca taggcttctt tcttttttaa aatagttaac caagatagcc
     8161 cagcatgttg tgattctaat gctaaaagtt taaacaatgc cttgctatca tagagcggtt
     8221 gtccccatac atgatcatga tagtctaagt agactggatc tttagtacca aatgcgcatt
     8281 cattcatttt attcccacct atacattgac ttattttgtc aatcattata ctataagtta
     8341 agagtttaat ataggatttg ggaagagtaa gttcaaatta ttttaaagag agcatatgat
     8401 ttggtgtgag tatgtaaatg atttttactg aaggtagccc acagagtatc ttagctgaac
     8461 gtgattaata gtaggctaag acgtgtttga gcgttaatca taatctagag tgcttgaaga
     8521 gatgaagcat ttcaaatttc acgatgatag tattggtatg attattccaa ttatgttaaa
     8581 agagtgaatt tgatattaat catccttctt gaatataggt ggtaccacgg aatatccgtc
     8641 ctatttgtat ataggatgga tatttttatt tttttaggag gtatagcaaa tggaaatgaa
     8701 accaaaatat gatcctcgtg aagttgaagc gggacgttat gaagaatggg taaagaatgg
     8761 ttattttaaa ccgtcagaag ataaatcaaa agaaacatat acaattgtta tcccgccacc
     8821 aaatgtaact ggtaaattac atttaggaca tgcatgggat acgactttac aagatatcat
     8881 tacacgtatg aaacgtatgc aaggatacga tacgttatac ttaccaggta tggatcatgc
     8941 tggtattgcg acacaggcaa aggtagaagc taaattaaat gaacaaggaa taactagata
     9001 tgatcttggt cgtgaaaagt ttttagaaca ggcatgggat tggaaagaag agtatgcgtc
     9061 atttattcgt gcgcaatggg ctaaattagg tctaggttta gattatagta gagaacgttt
     9121 tactttagat gaaggtttaa gtaaagcagt taaaaaagtt tttgttgatt tatacaataa
     9181 aggaattatt tatcgtggcg aacgtattat aaattgggat cctaaagcac gtacagcttt
     9241 atctgatatt gaagtaatac atgaagatgt tcaaggtgcg ttttatcatt ttaaatatcc
     9301 ttacgctgat ggtgaaggtt ttattgaaat tgcaacaaca agaccagaaa cgatgttagg
     9361 tgatacagcg attgttgtta accctaatga cgaacgatac aaagatgtaa tcggtaaaac
     9421 tgttatatta ccaatcgtag gacgcgaact gcctatttta gcagatgagt atgttgatat
     9481 agacttcggt tctggtgcta tgaaagtgac accagcacat gaccctaatg attttgaaat
     9541 tggtcaaaga catcaattag aaaatattat cgttatggat gaaaatggta aaatgaacga
     9601 caaagcgggt aaatatgaag gtatggaccg ttttgattgt cgtaaacagc tagttaaaga
     9661 tttaaaagaa caagatttag ttatcaagat tgaagatcat gttcattctg taggtcattc
     9721 agaacgatct ggcgctgttg ttgaaccata tttatcaaca caatggtttg tgcgcatgga
     9781 agacttagcg aaacgttcat tagataacca aaaaacagat gatcgtattg atttttatcc
     9841 gcaacgtttc gaacatacat ttaaccaatg gatggaaaat attagagatt ggacgatttc
     9901 aagacaatta tggtggggtc atcaaattcc ggcttggtat cataaagaaa caggcgaaat
     9961 atatgttgga gaagaagcgc caactgatat tgaaaattgg caacaagatg aagatgtatt
    10021 agatacgtgg ttctcaagtg ctttatggcc tttctctacg ttaggttggc ctgatttaga
    10081 aagtgaagac tttaaacgat actacccaac aaatgcctta gttacaggtt acgatattat
    10141 ctttttctgg gtagcacgca tgatattcca aggcttagaa tttacagatc gtcgtccatt
    10201 taatgatgta ttattacacg gtttagttcg tgctgaagac gggcgtaaga tgagtaaatc
    10261 attaggtaat ggtgtggatc caatggatgt tattgacgaa tacggtgctg atagcttgcg
    10321 ttacttctta gcaacaggtt catctccagg acatgattta agatactcaa ctgaaaaagt
    10381 tgagtcagtg tggaacttta tcaataaaat ctggaatggg gcacgtttca gtttaatgaa
    10441 tatcggtgaa gactttaaag ttgaagatat cgatttaagt ggtaacttat cattagcaga
    10501 taaatggatt ctaacacgtt taaatgaaac gattgcaaca gttactgatt taagtgacaa
    10561 atatgaattc ggcgaagttg gacgtgcatt atataatttc atttgggatg atttctgtga
    10621 ttggtacatt gaaatgagta aaattccaat gaatagtaat gatgaagaac aaaaacaagt
    10681 tacacgttca gtattgagtt atactttaga caatattatg agaatgctac atccattcat
    10741 gccatttgta acagagaaaa tatggcaaag tttaccacat gaaggtgaca caattgttaa
    10801 agcttcatgg ccagaagtgc gtgaatcatt gatttttgaa gaaagtaaac aaacaatgca
    10861 acaacttgtt gaaatcatta aatctgtaag acaatcacgt gtagaagtaa atacgccatt
    10921 gtctaaagaa atacctattt taattcaagc taaagataaa gaaattgaaa caactttatc
    10981 acaaaacaaa gattatttaa tcaaattctg taatcctagt accttaaata ttagcactga
    11041 cgtggaaatt cctgagaaag caatgacatc agttgtaatt gcaggtaaag tggtattacc
    11101 attagaaggg ctaattgata tggataagga aatcagccgt ttggaaaaag aattagctaa
    11161 acttcaaagc gaattagata gagtagataa aaagctctct aatgaaaact ttgtaagtaa
    11221 agcacctgaa aaggttataa atgaagaaaa acgtaaaaaa caagattatc aagaaaaata
    11281 tgatggtgtg aaggcaagaa ttgaacaatt aaaagcatag gagttagtaa caatgaatta
    11341 cctagagagc ttgtattgga tacacgaaag aactaaattt ggcatcaaac caggtgttaa
    11401 acgtatggaa tggatgctag cacaatttaa taatcctcaa aataacatta agggtattca
    11461 tgtaggtggc acaaatggta aaggctctac agttgcttac cttagaacag ctttagttga
    11521 aaatggttat gaagtaggta catttacgtc gccgtttatt gaaacattta atgaacgaat
    11581 tagtctaaat ggtgtgccaa tatcaaatga cgctattgta gaattagtat cacgtattaa
    11641 accagtaagt gaaatgatgg aacgtgaaac agatttaggt gttgcaactg aattcgaaat
    11701 aatcacagcg atgatgtttt tatattttgg tgaaatacat cctgttgatt ttgtcattgt
    11761 tgaggctgga ttgggtataa agaacgattc gacaaatgtc tttacaccgg ttttatcaat
    11821 cttaactagt atcggtctag accatacaga tattttaggt ggtacttatc tagatattgc
    11881 tagggataaa ggcgcgatta taaagcctaa cgttccagtg atatatgctg ttaaaaatga
    11941 agatgcatta aaatatgttc gtgaacgcgc aattgaacaa catgcaaagc caattgaatt
    12001 agatagagaa attgttgttg tatcgcaaaa tgatgaattt acttaccgtt ataaagatta
    12061 tgaattagaa acaatcattt taagcatgtt aggtgaacat cagaaacaaa atgctgcatt
    12121 agccataaca gctcttattg aattaaatga acaaggatta attgaattag atttcaataa
    12181 gatgatagac ggtattgaat cagttcgttg gactggacgt attgagcagg tgcatgacaa
    12241 acctttaatc attttggatg gcgcacataa ttcagagagt atagatgctc taattgatac
    12301 aattaaacag taccatgata aagaaaaagt agatattttg ttctcagcaa taaacggaaa
    12361 accgattaac gagatggtca aacatttaag tttaattgcg catacgtttt atgcaactga
    12421 atttgatttt ccgaaagcgt tacgcaaaga agaaattgta ggtagtattg aaaatgatga
    12481 aatacaatta gtagatgact acgttgaatt tataaaaaat tatcaaggtg atacattagt
    12541 aattaccggt agtctgtatt tcataagtga agttaaatca aaattagact ttgaagcatt
    12601 ataaaactaa aactaaaata gccatgaaga atctatttaa tcaataggta gagcttcatg
    12661 gttatttttt atgctctaaa tcgtatgtgt ttataaggtt caatttcttg ccattattaa
    12721 ttttgccgag cagtaatggg ttgttaaagt atccatcaat tatgcttagc aatattgaaa
    12781 aatttaaatg aaaaagcggt tcaattcttg aaaaatagtg tatatttaca ttttggtaat
    12841 aactaactat aatttacata aggaggttgt catttggtag tattgttatc ttatagttgc
    12901 agttgtattt ttagttttct atatcaattt atatctatag aagaaacgtc atttgattat
    12961 ttacatagaa gatcgaaatg tgattattgt aattcgtcac tcaaatggta tgaattaatg
    13021 ccgattatta gttttttatt attaaaaggg cgatgtcgaa actgtcgaaa gcgtatttcc
    13081 ctaacacatt tcttagggga aacctttgct ttaataccta tcgtctttat taagtatgat
    13141 ttcacatacg taaatgctac gctatttata actacatatg tttttctgct tatttttact
    13201 atgaccgata tcacttcttt aatgttagat tgtcgcttaa ttataattta ttgtatcgtt
    13261 tctctctcgt taagtatgat ttatccagta gcttttatca ttattagtat gaccacgcat
    13321 atattctact ttttatttcg ggcatatatt ggttatggtg acgttttact aatatctgca
    13381 ctttctttgt ttttccctct ccaattcact atttatgtca ttttatttac atttgtcatt
    13441 gctggtttag ttgctttaat taccatgata tttaagccga ttaaactatt accccttgtt
    13501 ccatttatat ttatttcctt ttttatcaat tcactttttt ataatgatat ccatcaattt
    13561 ttaggaggcg tatatttttg aaaattaaag aaatggtaac ttcagaaatg ccaagagaac
    13621 gtttgttaag ccatggtgca aaaagtcttt cgaatacaga attattagct atattaatta
    13681 acaccggaag aaaaggattc tcgagcatag acattagtaa tgaactgctt aaatctgctt
    13741 cgaatctgaa tgaattgaaa aaatcttcaa ttaacgattt gataaaagtt aaagggattg
    13801 gattacaaaa agcaattact ttaaaagcag catttgagtt aggggaaagg atggggagaa
    13861 gagctgaaaa taatcgtata aaaataacgc aaccaagtga tgttgctgat tatatgattc
    13921 caacaatgaa agatttaaca caagaacatt ttgtcatttt attgttaaat tcaaaaaatg
    13981 tagtgattaa agaaacctgt gtttttaaag gtacattaaa tagttcgatt gtacatccac
    14041 gtgaaatttt tagtattgcg gtgagagaaa atgccaatgc aatcatcgca gttcataatc
    14101 atccatccgg tgatgtaacg ccctcacaag aagatatcat aacaacaatg aggttgaagg
    14161 agtgtggttt gattttaggg atagatttat tggatcatat tataatcggt gataatagat
    14221 ttaccagtct tgtagaagcg ggttactttg atgaaaatga ttgaagttga tagaattaat
    14281 gacgtatctt gtgtataata cctacgaagt actttcattg gaggaaaaat agtgactcta
    14341 tttattatta tcggggttct cgtgccaatg gtttatacca tgcagttaaa tattaaaaat
    14401 gaacctgtaa caaagcgcaa tcttttaata acattagctt tatctacgtt aggtatttta
    14461 gtaaccgcgt tagcaggtgt aatcgttacg aaacaagctt ttcctttatt aagtgtagca
    14521 attggctcaa tttttactgg aatcgtttgg ggccttttac taagtggtag ctacgcgctg
    14581 atacgatttt tatctaacgc atttgggcgt aagtaatagt aatctgttca tcaagtagta
    14641 tccgtgcttg aaaacaaact aaaactccta atgtggaact agttaaaaaa ttctaaacta
    14701 gtacattagg agttttgtta tgcagaataa atttaattgt taaattgaaa gtgcggtaaa
    14761 aatccactat ttatttgaaa aaaatcgagc aaccaaatta aaccttgata tcctaagtaa
    14821 atacataata aaacaagtcc gacataaatt aaaaatcgca aaatagaaag tccaactcta
    14881 aaaaggagga tgactaatag tgctattaaa ataattgtta gtatactcaa tgctacaaac
    14941 ctcctaatac gcttttaaat cataacaaaa ttataagcta ttttaaatga acgtgcatca
    15001 gtcctaagat atatcatgat taaggctgaa gactgaggaa aatatacata ataaaatata
    15061 taattagatg aagacattgg aggcgaacta tgagattgat atttagtatg attaaaaaca
    15121 ttatagcagt aatagcaatt gtgataattg tttatatcgc cttgaaatac gcaccttttt
    15181 taaaagaaca agattggaat cctgtaaatc acgataatga tcaaatgaat caagttacgc
    15241 aacccactaa tgatgcccaa catgtttatg taagtggtga gaaatattca ttaaaagaaa
    15301 atgatttaat aaagaatgta ccattaagcc aaattaaaaa tgtatttaaa atgatagata
    15361 aacaagagtt tatggctgtg tctggaatga atcgcatggc ttataatgat caatatatta
    15421 taggtcaaag aggagacgaa tttattcttt ataaatttgg agatgagtca atgcgtgttt
    15481 acaatactga atttgaaatg caacaagact taaatgaatt agggcaaaat ttacaactaa
    15541 aatctgaaaa tgcttatcaa taggaaatta ttgcataaga aatatatttt agttatcctg
    15601 tcactagaat aaatttaaaa ggttggtgta gttcaaatgg cagaacaatc aaaacagaaa
    15661 caagctaatg aacaacaaaa ggcccagaac ttattcgcac gttggagaca acttcagaat
    15721 tcgaacagtg aatcctcgaa cgatacaaac aaataatttt aaaatttaaa tcgattattt
    15781 aaactactag tgactggaat gtttgctagt agtttttttg tttttaaaat gccacaaatt
    15841 ttttacggag ttgttcattt tcaaagtaat tcgtagttag tgctttattt cataatggga
    15901 gtaatataaa atagaaaagg atatacaata ttttgacata atagaggtgt tctgggtgct
    15961 taagtttttt aaaaataaca aattaattgt tgttttatgt gcaattatcg tttttattgc
    16021 attaattggg ctgtccatac gttcacaatc tcaatcacct cctgaacaat atataggtga
    16081 ttctgtgtct tttggacaac gagttgtgag ttatccagtt aattttgttg ctggtacgat
    16141 tggggacttt tttaaaaaag gagattctaa agaatctaaa aataagatta gccagttaga
    16201 atctaagaac caacaattag aagcggaaaa tgaaaaatta aaaaaagagc ttgatttaaa
    16261 agatatttca aaatttgatc ctatttctac tacggttttg gcaagaaatc cggatcagtg
    16321 gatgaataca attgtaattg ataagggatc taaatcaggt ataacttcaa atatggctgt
    16381 gatgacatca caaggttttg ttggaagagt tactaaagtt aataaatttt cttcacaagt
    16441 tgatttaatc tcaactaata cacgtgcggg taaattatct gtaaatatac aacacggttc
    16501 taaaaatata tttggtttaa ttgatcgtta tgatgaaaag aactcagaac ttgtaattag
    16561 tgacattaat aatagagata atatctcaaa aggtgataaa gtcgttacaa gtggattagc
    16621 tgatcaacta ccaagtaatt tatatatagg agaagtgact aaggttcaaa atgatcaata
    16681 cggcttagct aaagaagtta gggttaagac tggtgcggac ttaacagatt tgagtcatgt
    16741 ttatgttgca aaaagagatc ctaaaacaat tcctgatgat gaaagcaggg ataaataatg
    16801 cgtacactgt attatttttt gataggtatt ttactatttt atatagatac tgcaattggg
    16861 cttcttattc caatgcacat aggtaagttt gaacttgtat ttgtaccaca ccttacattt
    16921 atgtatattt taatgatggt agtgtatcgt ggctttggcg tatcattatt gctcagcata
    16981 tttttaggcg taatgactga tgtatacttt ggtagtattt atggagtgta cttatttggc
    17041 tatatattgt ttttagcact tatagatcga ttctttaaaa ttttttacaa agatcattcg
    17101 atgttattca ttattatatt agccagcacc ttattattag aagtctatgt ggcattaata
    17161 tacggtatgt taggattcat tcaatttgat attattcatt ttgtagtctt tagattattg
    17221 ccaacattaa ttatgaattt tgtactgttg attatgcttt atccgttgat tataaagttc
    17281 cttaaaaaaa caaacaatga cattgacatg aaacgtcgtc aatggtaaga tgcaatagtt
    17341 gagtagttat aagtagctac atacaaccgc tcaaatatag gttcaagaac atttttaatg
    17401 aagaaagcga ttttcccaca gagtgcaaac gcttgactca ataacgaaat gtcacctatg
    17461 tatggcgtga cttattatac aggaggtgca aagtatgttt gctattattg aaacaggtgg
    17521 aaaacaaatc aaagtagaag aaggtcaaga aatcttcgtt gaaaaattag acgtaaacga
    17581 aggagatact tttacatttg ataaagtatt atttgtaggt ggagattcag ttaaagttgg
    17641 agcgccaaca gttgaaggtg caacagttac tgctactgtt aataaacaag gtcgcggtaa
    17701 aaaaatcact gtattcacat acaaacgtcg taaaaattca aaacgtaaaa aaggccatcg
    17761 tcaaccatac actaaattaa caatcgataa aatcaacgcg taattattat gattactgtt
    17821 gatattacag ttaatgatga aggcaaagta acagacgtta ttatggatgg ccatgctgac
    17881 catggtgaat atggtcatga tatcgtttgt gctggagctt cagctgtatt gtttggtagt
    17941 gttaatgcga ttataggatt gacatctgag agaccagata tcaattatga cgacaatggt
    18001 ggtcattttc atataagaag cgttgataca aacaacgatg aagcgcaact aattcttcaa
    18061 acaatgcttg tgtctttaca aactattgaa gaagaatata atgagaatat tagattaaat
    18121 tataagtgag gtgcattccg atgttaaaat taaacttaca attcttcgca tctaaaaaag
    18181 gggtaagttc tacaaaaaac ggacgtgact ctgaatcaaa acgcttaggt gctaaacgtg
    18241 ctgacggtca attcgtaaca ggtggttcaa ttttatatcg ccaacgtggt actaaaattt
    18301 accctggtga aaatgtaggt cgtggtggcg atgatacatt attcgctaaa atcgacggcg
    18361 ttgttaaatt cgaacgtaaa ggtcgcgaca aaaaacaagt ttctgtatat gcagtagctg
    18421 aataattttg tctagttaac accagaagtg aatcttctgg tgttttttac tttttataaa
    18481 atgtttttca ttattatttt cattatgtta ttaaaaaatg ggaattttag acgttatact
    18541 aaatgtgcaa ctgtataaga gcccctaatc actaaactat aagggggaca aaggaataca
    18601 gttgcagcgt ttaaagaata aactgtacca caattggtgc tgagaaatat aagtatttta
    18661 aagcaaaga
//
LOCUS       DAKTGS010000043        18654 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.043, whole genome shotgun sequence.
ACCESSION   DAKTGS010000043 DAKTGS010000000
VERSION     DAKTGS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.043"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            98..2140
                     /locus_tag="O0286_001254"
     CDS             98..2140
                     /locus_tag="O0286_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0169638.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(3300..4229)
                     /locus_tag="O0286_001255"
     CDS             complement(3300..4229)
                     /locus_tag="O0286_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCY0169639.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(4226..5062)
                     /locus_tag="O0286_001256"
     CDS             complement(4226..5062)
                     /locus_tag="O0286_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0169640.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(5056..5799)
                     /locus_tag="O0286_001257"
     CDS             complement(5056..5799)
                     /locus_tag="O0286_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0169641.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            5921..6565
                     /locus_tag="O0286_001258"
     CDS             5921..6565
                     /locus_tag="O0286_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCY0169642.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(6645..7397)
                     /locus_tag="O0286_001259"
     CDS             complement(6645..7397)
                     /locus_tag="O0286_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCY0169643.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            7633..8397
                     /locus_tag="O0286_001260"
     CDS             7633..8397
                     /locus_tag="O0286_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCY0169644.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(8458..9252)
                     /gene="tagH"
                     /locus_tag="O0286_001261"
     CDS             complement(8458..9252)
                     /gene="tagH"
                     /locus_tag="O0286_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCY0169645.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            9599..10411
                     /gene="tagG"
                     /locus_tag="O0286_001262"
     CDS             9599..10411
                     /gene="tagG"
                     /locus_tag="O0286_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCY0169646.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            10510..11613
                     /locus_tag="O0286_001263"
     CDS             10510..11613
                     /locus_tag="O0286_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0169647.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            11610..12671
                     /locus_tag="O0286_001264"
     CDS             11610..12671
                     /locus_tag="O0286_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCY0169648.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            12734..13132
                     /gene="tagD"
                     /locus_tag="O0286_001265"
     CDS             12734..13132
                     /gene="tagD"
                     /locus_tag="O0286_001265"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCY0169649.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(13249..14544)
                     /gene="pbp4"
                     /locus_tag="O0286_001266"
     CDS             complement(13249..14544)
                     /gene="pbp4"
                     /locus_tag="O0286_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCY0169650.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            14965..16692
                     /locus_tag="O0286_001267"
     CDS             14965..16692
                     /locus_tag="O0286_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169651.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            17051..18280
                     /locus_tag="O0286_001268"
     CDS             17051..18280
                     /locus_tag="O0286_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0169652.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            18535..18627
                     /locus_tag="O0286_001269"
     CDS             18535..18627
                     /locus_tag="O0286_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169653.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
BASE COUNT         6372 a         2721 c         3227 g         6334 t
ORIGIN      
        1 ttgtggtcgc taactcaatt tatcctctaa agtataatta caacgagatg tgatacttaa
       61 ttttcaatta aacaatactt tttagagagg tgaaagtttg gaaatatttg aaacaattct
      121 tatatttata gctgttgtga tactaagttc gtttgtccat actttcatac ctaaagtacc
      181 cctagcattt atacaaattt tcttgggcat gttactattt attaccccaa tccctgttca
      241 atttaatttt gattctgaat tgtttatggt aacaatgatt gcgcctttgt tatttgtaga
      301 aggtgttaat gtttctagag tccatttaag gaaatatatt aagccagtga tgatgatggc
      361 attaggatta gtcattacta ctgtgatagg tgtaggttta tttattcatt ggatttggcc
      421 agatttacct attggagcag catttgcaat tgctgccatt ctttgtccta ctgatgcagt
      481 agcagtgcaa gcaatcacta aaggaaaggt cttgccaaaa ggagcaatga caattcttga
      541 aggtgagtca ttattgaatg atgctgctgg tattatttca tttaaaatag ctgttggagt
      601 attagttaca ggtgcttttt cacttgttga tgctgttcag ttgtttttaa ttgcatcaat
      661 tggtggcgca gtggttggtt tacttatagg tatggcatta gtaaggttcc gattaacatt
      721 gatgcgtcga ggatatgaaa acattaatat gtttacaatt attcaattgt taacaccatt
      781 tgttacgtat ttaattgctg aattgtttca cgcatcagga atcattgcag cagtagttgc
      841 aggacttgta catggtttcg aacgtgacag aattatgcaa gtacgtacac aactgcaaat
      901 gagttacaat catacatgga atatactagg ttatgtttta aatggctttg ttttttcaat
      961 attaggattt ttagtacctg aagttattat taaaattatc aaaacagaac cgcacaattt
     1021 aatcttttta ataggcatca ctattgttgt tgctttagct gtctatctat ttagatttgt
     1081 ttgggtttat gtcttatatc cttattttta tttagccatc agtccattcc aaaaaatgat
     1141 gactaaaaat gatgatgata atccaacgac tgagaaacca ccaaagcgaa gtttatacgc
     1201 tttaattatg acgttatgtg gtgtgcatgg aacaatttct ttagcaatcg cattaacgtt
     1261 accgtatttt ttagcagggc atcatgcttt tacgtataga aacgacttat tatttattgc
     1321 atctggtatg gttattatta gtttggtagt tgcgcaagta ttattgccat tattaacgaa
     1381 acctgcacct aaaacagtaa ttggcaatat gtcgtttaaa gttgctagaa tttatatatt
     1441 agaacaagtt attgattatc taaatcaaaa atctactttc gaaacaagtt ttaaatatgg
     1501 taacgtgatt aaagaatatc atgataaatt agcattttta aaaactgtag agaaagatga
     1561 tgaaaactct aaagaattag aacgtctaca aaaaattgct tttaatgtag aaacaaaaac
     1621 attagagtct ttagtagatg aaggacaaat aacgaatagt gtacttgaaa actatatgcg
     1681 ttatgctgaa agaacacagg tatatagaca agcatcatta ataagaagaa tgattgtatt
     1741 attacgaggt gctttattaa aacgaagagt acaaacgaga gtgaactccg catcttcact
     1801 tagtgttacg gataacttaa tggaattaaa taaaattaat aaattagtcc attataatgt
     1861 ggttagtcgt ttgtctaagg aaacaacaaa agataataca cttgaagttg gaatggtttg
     1921 tgacggttat ttaatgcgaa ttgaaaactt aacaccatca aatttcttca actcagcaag
     1981 tgaagatacg attactaaaa ttaaattaaa tgcattgaga gaacaacgtc gcattttacg
     2041 tgagttgatt gatacagatg aagtatcaga aggtacagcg ttaaaactaa gagaagccat
     2101 caattatgat gaaatggtta ttgtagatag tatgacgtag ttcctaatta tgctaaaagg
     2161 gattgatgaa aaactgaagg gcttttcatc aatccctttt attttagggg aattgaatag
     2221 atagttttaa actatacgaa ttattaatat ttgagattta attgaaataa gttttaaaaa
     2281 ttggaggaga tagattaagc gaagtcattt aaaggtgaag ttaagtgtat tcacaaaaaa
     2341 tagccacact catatgacat cggatgagtg tggcttaagg atctatgggg ggaggaaacc
     2401 atagatgttt actttgatag gccagattaa atatcaaagt atgcgattat ttatagcttg
     2461 atgcaaaagt ggtatgccta ttaaaagtta ctgcacatag cttttaatat tccgttcaaa
     2521 ggaaaggggc atacaattga acaatctgta atagtacttt taaccagcta tgctaaaagt
     2581 ctagtaggga gaacagttgt ccaatcacat aagaacctct aacttcgtta gtacgattaa
     2641 gaaaagcttt ttagttagta tgtaatacaa tttattgacg cgcgtgaatc tcttttataa
     2701 gagtgtgtag ggaatggcgt tgtataaatt gtattagaag aacttctaac gcatctctgt
     2761 ggttaaaaga gatgaaggga acgacagttt aattaaaact gcataagaac ttctagcttt
     2821 tctctctcgt tcaaagagaa gcagctgttc gcagtttaat caaaaccaca taaagctttt
     2881 aactttactc tttgatttaa agagtgataa atgtttacag tttaattaaa actgcataag
     2941 aacttctagc ttttctcttt cgttcaaaga gaagcagctg ttcgcagttt aatcaaaacc
     3001 acataaagct tttaacttta ctctttgatt taaagagtga caaatgttta cagtttaatt
     3061 aaaactgcat aagaacttct agcttttctc tttcgttcaa agagaagttc taataccacc
     3121 atatcgtgcg atcgggaacg gtatatatat taataggagg gtaatatata tttaacgcac
     3181 gatatgggac tattagcctt cgactttgtt atgttgatgt gtggcctaaa atattggaga
     3241 taccaatatt ttaggttgca tcaacatcaa ttcatcttac ttcattaaaa cacagcgtgt
     3301 tatttcatgc ttccgtgtac agtttcaata tttgatttca tcattttgta gtaagagtca
     3361 cctttagtgc cttctttacc gattgaatct gtgtacactt caccaaagat atctttcttc
     3421 gtttcttcag ataaactttc cattgctttc ttatcaacac ttgtttctac taataagtgt
     3481 tttaatttgt gctttttaac aaactcaata gcttgtctca tttgttcagg tgtaccttgt
     3541 ttttcagtgt taatttccca aatataacct ggtgtaatac cgtattgttt tgagaagtac
     3601 ttgaaggcac cttcacttgt aatcatggca cgttgttctt ttggaatgtc attaaatttg
     3661 tctttactgt cattatttaa tttttccaat tgagcaatgt atttgttacc ttgcttttca
     3721 taatctgctt tatgtttttt gtcgttatcg ataaatgttt gttgaattgt ttttacgtat
     3781 ttaataccgt tatctaaact taaccatgcg tgtggatctt gtttatcttt gttgccttct
     3841 tcaccgttta aatagatagg tttaacatct tttgatactg cgataacttt tttatctttt
     3901 aatgatttac cagcctgttc taaggctttt tcaaaccaac cgttaccagt ctctaaattt
     3961 aatccgttgt ataaaataac gtcagcgtca gttaactttt taatatcttt aggtttaact
     4021 tcatattcat gaggatcttg accaacaggt acaatactat gaatatcgac gttgtctcca
     4081 ccaacatttt tagccatatc atataaaatt gaattcgtcg ttactacttt taatttgcca
     4141 tttgacttat cactgctttg tttaccacca gtaccacatg cagcaactag aagtaataag
     4201 gctaataata aaggtactaa ttttttcatg ttaaacttcc tcgtttcttt ctattcgtaa
     4261 attttgtgaa aaataatgtg atgatataaa ttacaaacgt acaaagtacg attgtcgcac
     4321 cactaggaat gttgtaaata tagctgtaat aaagtccgac aattgaactt atgacactta
     4381 ttaaacttgc tataatcatc attgagtata gttttttact aattaaaaat gctgtagatg
     4441 caggtgtaat taataatgca actacaagaa taatacctac cgtttgaata cttgctactg
     4501 ttactaatga gagtaacaac atcacaaagt aatgtaataa cgtcgtattt agaccactca
     4561 ttctactaaa cgttggatcg aatgtagaaa tcattaatgg acgatagaaa ataatgatta
     4621 gaataaggac gattgaacca atcacaatag ttgttaaaaa tgcactattt gtgattgcca
     4681 gtaaattacc aaacagaata tggtacaaat ctgtcgtagt gtttattaag ctaataataa
     4741 taatccccga agctaagaaa gcggtaaaac taattccaat agcggcgtca ggtttcgttt
     4801 tactactaga tgtgatataa ccgataaaaa tacttgcgat cataccagtt ataagtgcgc
     4861 ctacaaacat tggaatacca aataagaatg atagggcaac accaggtaat actgcgtgac
     4921 tcattgcatc tcccattaat gaaagaccac gtaatacaat taaactacca actgtaccac
     4981 aaactatccc tacaataatt gaagttatca atgctcgatt caagaattga tatgtaaata
     5041 aatgttcgac aaactctaac atgttatatt gctcctttga ctagggtcac tacagtcagt
     5101 gctactcata aatgtttcgt ttaagcgagt gacactcata gcctcttcac tatcaccaaa
     5161 gtatcgtaat gtttgattta atagaataat gcgatcaaag tattgctttg cttttgatag
     5221 atcatggtgg atgataagaa taagttttcc ttgttgtttt aagttctcga tttttgtcat
     5281 gattaatttt tcgctactaa aatcaattcc gacaaacggc tcatctagaa aataaacttc
     5341 actttcggac atcaatgctc ttgctactag cacacgttgt aattgtccac cacttaattc
     5401 tgaaatttgt cgatgacgta aagattctaa ttctaaatcg cttaataact gtttgagttt
     5461 atcccttgct gatttattag gtcgtctaaa ccatccaatt tctttgtagc aacctgataa
     5521 aatcacttgt tccacactta taggaaaatc taaatcaata tgtgcttttt gtggaatata
     5581 tgtaatatgt tgcagttgtt gttgtatagg tttgttatat aacaatttag taccggtagc
     5641 attaaattca ccaattaaag acttgataag ggaagattta ccagcaccat tcgggcccat
     5701 gataccaatt atttcgccgc gtactggtat cgataaggaa atgtttttaa gtacatgctt
     5761 attacctaaa aacagattta aatcttttgt ttctaacaaa cgtttatacc tcctaattaa
     5821 aagtttaggc taacctaatt aattgtataa taaactgaga atatttatca tgtcaagtaa
     5881 attcgtgata taatatagac aatgtatgtg aggtgaaagt atgttaactg aagaaaaaga
     5941 ggactattta aaggcaatcc ttacgaataa tggcgataaa aactttgtga caaataaaat
     6001 cttatctcaa tttttaaata ttaagcctcc atctgtaagt gaaatggtag gacgtcttga
     6061 aaaagcaggc tatgttgaaa caaaaccata caaaggtgtt agattaacag aggatggttt
     6121 aacgcatacg cttgatatca ttaagagaca tcgactatta gaattatttt taatagaaat
     6181 attgaaatat aattgggaag aagtacatca agaagcagaa attttagaac atcgaatttc
     6241 agatttattt gttgaaaggc tggatagcct gttaaatttc ccagaaactt gcccgcacgg
     6301 cggtgtgatt cctagaaata atgaatataa agagaaatat ataacaacga ttttgaatta
     6361 tgaacctggt gatatcgtta caatcaaacg tgtgagagat aagaccgatt tgctaatata
     6421 tttgtctagt aaagatattt ctattggtaa tgaagtggaa attgtatcga aagatgaaat
     6481 gaataaagta attatcatta aacgtaatga taatgtaatt attgtcagtt acgaaaatgc
     6541 aatgaacatg tttgctgaaa aataaaataa agaagccata aagatatcca tgattgaact
     6601 gataaagaca tatggataat tgctttaggc ttctttttta ttagttaatt tatcaagtga
     6661 gtatatttga gtaaaatatt cactgcataa agattgaaga taatccagat tgtactataa
     6721 atgaagatag gtacatgact gagttcttta agtgcactac catcccactg tggactcgga
     6781 cgctggaaag tcaatttagc aatcgtccaa ctagattgta gaacttcgcc taataataca
     6841 cctaaaatat attgataact cattgtgaca agtagttgaa tttctactat attttcatct
     6901 tttaatataa aatacaacat gatagaaatt aaagttataa caacaatggg tgagcctttt
     6961 ctagatgtta aaattaaaaa ataaataaat atcaataaat aggtaaatat aaagaaacta
     7021 ggtatctgat aatggctcga cgctaaacct atcaataaca taataggtgg cataaaataa
     7081 ccaccaatcg ttgtaagcca ttggcctgct agatgtctag attgtgtaat tgcgaatcct
     7141 tgttgtaatg tctgttgtcg ctctcgtgga cttgttacaa tgactaaatc ttttgcacgg
     7201 ccaccagcga gtttattaaa cagtacatga ccaaattcat gtgttaaaac agggatatag
     7261 tttaaaatga catctaaata gttcaaaaca ggcttatgtc tatattgatg aatagcaata
     7321 taacaagctg caacaataac gataatgtat atattaagtt gaattgtcgt attaaaaaag
     7381 tttgataaat aattcattgt taacctcata taagatatta atttaaagtt tgcttatcac
     7441 ttattataaa tgatattggc atcaatagcg ttagacttta gacttacctt agttaaacta
     7501 attttaattt ttgaaaaggt gaatatgtgt taaaataaag caaaatcatt tcgatataaa
     7561 taggatgaat ataaatactg ttaatattga ttacactaac ataataatga aataagatag
     7621 gagattcctg ttatgactgt tgaagaaaga tccaatacag ccaaagttga cattttaggg
     7681 gtcgattttg ataatacaac aatgttgcaa atggttgaaa atattaaaac cttttttgca
     7741 aatcaatcaa cgaataatct ttttatagta acagccaacc ctgaaatagt gaattacgcg
     7801 acgacacatc aagcgtattt agagttaata aatcaagcga gctatattgt tgctgatggg
     7861 acaggagtag tcaaagcttc gcatcgttta aagcaacctc tagcgcatcg tatacctggt
     7921 attgagttga tggatgaatg tttgaaaatt gctcatgtaa atcatcaaaa agtatttttg
     7981 ctaggggcaa ctaatgaagt tgtagaagcg gcacaatatg cattgcaaca aagatatcca
     8041 aacatatcgt ttgcacatca tcacggttat attgatttag aagatgagac agtagtgaaa
     8101 cgaattaaac tgtttaaacc tgattacata tttgtaggta tgggattccc taaacaagaa
     8161 gaatggatta tgacacatga aaaccaattt gaatctacag tgatgatggg cgtaggtggt
     8221 tctcttgaag tatttgctgg ggctaaaaag agagcgcctt atatctttag aaaattaaac
     8281 attgaatgga tatatagagc attaatagat tggaaacgta ttggtagatt aaagagtatt
     8341 ccaatattta tgtataaaat agccaaagca aaaagaaaaa taaaaaaggc gaaataatca
     8401 tgatgacaaa aataaaaccg aggaaatcct taaatggaga ttctcggttt tttcggttta
     8461 tttaataacg aagcgggact catcgagttt gtttctaaat tctttttgtt cggctttgga
     8521 tttcttttta aaatcgttaa ggaaagcttc atatttaggt aatacatcat caagttcacc
     8581 gtaatctttt aactttccgc cttcaatcca agcaatctta gtacaaaatt gtctcacttg
     8641 tcctaagtta tgactaacga aaaagatggt tttgttttgc tctttaaact cgtaaatttt
     8701 atctaaacat ttttgtgcaa aagtttggtc acctacagat aaagcttcgt caatgactaa
     8761 gatatctgga ttaactgtga tattaattga aaaaccaagt tttgcacgca taccacttga
     8821 atacttttta actggttgat aaataaactc accaagttca ctaaattcaa taatcttagg
     8881 tgtcatcgct ttaatttctt ttcgcttaaa gcccatacat aacattttaa attcgatatt
     8941 ttcaatccct gtaagttgtc cactcaagcc agcactaatt gcgataacgc tgacttcacc
     9001 attacgatcc actttgccaa cagtaggcga caaagaaccg ccaatgatat tgctcaacgt
     9061 tgatttgccg gaaccattga tgccaacaag ccctatgacg tcaccttcat atgcttttaa
     9121 actaatgtca tctaaagcga aaaatgtttt gtttttatgt ttgggaatga gcgcatcttt
     9181 catacgttct ttatttgtac gataaatacg atattctttt gttacatttt taatgtttac
     9241 cgaaacgttc atttgtagac cttccttatt cacatttatc tagattataa tatactactc
     9301 aacagttgtt aaattttaaa acctgttgta aagtgtatag aagattttgt tattatcaga
     9361 gtgggtgttt tgacacaaaa tgttaatcat caatgataac aatgatattt aaaaactaaa
     9421 cttatttcaa cttacatgat tgtatactat aatgtatttg taataaacta atattttaaa
     9481 gaactagaca ataattttga tagcatccat gtatagtgat agtatttaca acaattatta
     9541 taatactatt tagttaagta gagaaatagt taaacatttg aaagtgtggt ttaatggaat
     9601 gtcagcaata ggaacagttt ttaaagaaca tgtaaagaac ttttatttaa ttcaaagact
     9661 ggctcagttt caagttaaaa ttatcaatca tagtaactat ttaggtgtgg cttgggaatt
     9721 aattaaccct gttatgcaaa ttatggttta ctggatggtt tttggattag gaataagaag
     9781 taatgcacca attcatggtg taccttttgt ttattggtta ttggttggta tcagtatgtg
     9841 gttcttcatc aaccaaggta ttttagaagg tactaaagca attacacaaa agtttaatca
     9901 agtatcgaaa atgaacttcc cgttatcgat aataccgaca tatattgtga caagtagatt
     9961 ttatggacat ttaggcttac ttttacttgt gataattgca tgtatgttta ctggtattta
    10021 tccatcaata catatcattc aattattgat atatgtaccg ttttgttttt tcttaactgc
    10081 ctcggtgacg ttattaacat caacactcgg tgtgttagtt agagatacac aaatgttaat
    10141 gcaagcaata ttaagaatat tattttactt ttcaccaatt ttgtggctac caaagaacca
    10201 tggtatcagt ggtttaattc atgaaatgat gaaatataat ccagtttact ttattgctga
    10261 atcataccgt gcagcaattt tatatcacga atggtatttc atggatcatt ggaaattaat
    10321 gttatacaat ttcggtattg ttgccatttt ctttgcaatt ggtgcgtact tacacatgaa
    10381 atatagagat caatttgcag acttcttgta atatatttat atgacgaaac cccgctaacc
    10441 attaataaat ggaagtgggg ttcatttttg tttataattt aagtaaataa catattaagt
    10501 tggtgtatta tgaacgtttt aataaagaaa ttttatcatt tggtagttcg aatactttct
    10561 aaaatgatta cgcctcaagt gattgataaa ccgcatatcg tatttatgat gacttttcca
    10621 gaagatatta agcctatcat caaagcatta aataattcgt cgtatcagaa aactgtttta
    10681 acaacaccaa aacaagcgcc ttatttatct gaacttagcg acgatgttga tgtgatagaa
    10741 atgactaatc gaacattggt aaaacaaatt aaggctttga aaagcgcgca gatgattatt
    10801 atcgataatt attacctatt actaggtgga tataataaga cttctaatca acacattgtt
    10861 caaacgtggc atgcaagtgg tgcattaaaa aactttggct taacagatca tcaagtcgat
    10921 gtgtctgaca aggcaatggt tcagcagtac cgtaaagttt atcaagcgac ggatttttac
    10981 ttagtgggtt gtgaacaaat gtcacaatgt tttaaacagt ctttaggtgc aacagaagag
    11041 caaatgctgt attttgggct tccgagaatt aataaatatt acacagctga tagagcaacg
    11101 gttaaggcag agttaaagga taaatatgga attacaaata agttggtatt atatgtacca
    11161 acatatagag aagataaagc agataatagg gctattgata aagcttattt tgaaaaatgt
    11221 ttaccaggat atacactgat taataaatta catccatcaa ttgaagattc agacattgat
    11281 gacgtatctt caatcgacac gtctacatta atgctaatgt cagatataat tattagcgac
    11341 tatagttcgc tgccaataga agctagcttg ttagatattc caactatatt ttatgtgtat
    11401 gatgaaggaa catatgatca ggtgagaggc ctgaatcaat tttacaaagc aataccggat
    11461 agctacaaag tgtatactga agaagattta ataatgacga tacaagaaaa agaacatcta
    11521 ttaagtccgt tatttaaaga ttggcataag tataatactg ataaaagttt acatcagctc
    11581 acagaatata tagataagat ggtgacaaaa tgaggtttac gataatcata cctacatgta
    11641 ataatgaggc aacaattcga caattgttaa tatctattga gagtaaagaa cactatagaa
    11701 tcctttgtat tgatggtggt tctactgatc aaacaattcc tatgattgaa cggttacaaa
    11761 gagaactcaa gcatatttca ttaatacaat tacaaaatgc ttcgatagct acgtgtatta
    11821 ataaaggttt gatggatatc aaaatgacag atccacatga tagtgacgca tttatggtca
    11881 taaaaccaac atcaatcgta ttgccaggta aattagatag gttaactgct gctttcaaaa
    11941 ataatgataa tattgatatg gtaatagggc agcgagctta caattaccat ggtgaatgga
    12001 aattgaaaag tgctgatgag tttattaaag acaatcgaat cgttacatta acggaacaac
    12061 cagatttgtt atcaatgatg tcttttgacg gaaagttatt cagtgctaaa tttgctgaat
    12121 tacagtgtga cgaaacttta gctaacacat acaatcacgc aatacttgtc aaggcgatgc
    12181 aaaaagctac ggatatacat ttagtttcac agatgattgt cggagataac gatatagata
    12241 cacatgctac aagtaacgaa gaagatttta atagatatat cacagaaatt atgaaaataa
    12301 gacaacgagt catggaaatg ttactattac ctgaacaaag gctattatat agtgatatgg
    12361 ttgatcgtat tttattcaat aattcattaa aatattatat gaacgaacac ccagcagtaa
    12421 cgcacacgac aattcaactc gtaaaagact atattatgtc tatgcagcat tctgattatg
    12481 tatcgcaaaa catgtttgac attataaata cagttgaatt tattggtgag aattgggata
    12541 gagaaatata cgaattgtgg cgacaaacat taattcaagt gggcattaat aggccgactt
    12601 ataaaaaatt cttgatacaa cttaaaggga gaaagtttgc acatcgaaca aaatcaatgt
    12661 taaaacgata acgtgtacat tgatgaccat aaactgcaat cctatgatgt gacaatatga
    12721 ggaggataac ttaatgaaac gtgtaataac atatggcaca tatgacttac ttcactatgg
    12781 tcatatcgaa ttgcttcgtc gtgcaagaga gatgggcgat tatttaatag tagcattatc
    12841 aacagatgaa tttaatcaaa ttaaacataa aaaatcttat tatgattatg aacaacgaaa
    12901 aatgatgctt gaatcaatac gctatgtcga tttagtcatt ccagaaaagg gctggggaca
    12961 aaaagaagac gatgtcgaaa aatttgatgt agatgttttt gttatgggac atgactggga
    13021 aggtgaattc gacttcttaa aggataaatg tgaagtcatt tatttaaaac gtacagaagg
    13081 catttcgacg actaaaatca aacaagaatt atatggtaaa gatgctaaat aaattatata
    13141 gaactatcga tactaaacga taaattaact taggttatta taaaataaat ataaaacgga
    13201 caagtttcgc agctttataa tgtgcaactt gtccgttttt agtatgtttt attttctttt
    13261 tctaaataaa cgattgatta tcatatgaac aataagtgct aatccagcga caaggcatgt
    13321 accaccaatg atagtgaata atggatgttc ttcccacata cttttagcaa cagtatttgc
    13381 cttttgaata attggctgat gaacttctac agttggaggt ccataatctt tattaataaa
    13441 ttctcttgga tagtccgcgt gtactttacc atcttcgact acaagtttat aatctttttt
    13501 actaaaatca cttggtaaaa catcgtaaag atcattttca acataatatt tcttaccatt
    13561 tatcctttgc tcacctttag acaatatttt tacatattta tactgatcaa atgagcgttc
    13621 cattaatgca ttccccatca tattacgttg cttctcgcca ccaaggtttt tatagtctcc
    13681 tgcacccatg ataacttgat taattctaaa tttacctcgt ttggtagtaa tcgtatggtt
    13741 gtaatttgct gtatcacttg atccagtttt taaaccatct gtacccggca aactcatttt
    13801 tgcaccttcc aatgaaaagt tgaatgtgta atacgtaact gcatgcgttg ttggtgctaa
    13861 ctgctttgta aagtctaata ttttaggtgt ctctttaatc acgtgtaaat ctaaaatggc
    13921 atagtctcta gcagtcgtta cagtacgttc ttggtcttta tactttgttg gtgcaaatgt
    13981 acgtaatctt gaattttcag cacccgttgg attgacgaaa tgtgtatttt tcattccgat
    14041 agctttagct ttgttattca ttaaatcaac gaaatcgctg gtgttttttg aaaccttctt
    14101 agctaaaatt aatgccgcgg cattactaga attagatact gtaatttgta ataggtctgc
    14161 gattgtccat acttgtccag gatatagttt cgtattactc aactcaggta gtgtagacat
    14221 aatatattct ttgttcgtca ttgtgactgt gtcatcaagt gaaagctgcc ccttatttac
    14281 agcttccaat gttaagtaca ttgtcattaa tttagtcata gacgctggat tccacttagt
    14341 atcgatattg tattgataca gtaattgtcc agtttgactt acattaacag cactcgtcgg
    14401 ttcgtatgca gccgacaaac ctgcataacc atattgattt gctgcttgta caggggttac
    14461 gtcactgtta gtagcttgtg catatggtgt cataatactt aatgttaaac ataaaatgat
    14521 gataatagat attaaatttt tcataaagcg ttaatcttcc cttttccaat tcttaaatat
    14581 tccctaaaag caatggttat tcctacttac ggaaatcatt gctaattcac ttcaccttaa
    14641 ttaaattgtt gaaaataaag ttttctgcag ttaatttgaa aaataatgca aatatattac
    14701 gtgtgtagct aaaggtgtta taatgtttgt acgaagagca aacttactca aaagcgatta
    14761 attttcatgt tttaatataa agactttgag aagttattac aaaaaatgca atagaaatat
    14821 tctatcatat aaatgttatg agcggtattt tggggcaaca ctttatttga tttttaaagt
    14881 tttgttggga gaaagtatat gatagaaatg catgtatcta tctaaatgaa ttaactataa
    14941 atttcaaaca gaagaggtaa aactatgaaa cgagaaaatc cattgttttt cttatttaaa
    15001 aaactatcat ggccagtggg tcttatcgtt gcagctatca ctatttcatc actagggagc
    15061 ttaagtggac tattagtgcc actgtttact ggacgaattg tagataaatt ttccgtgagc
    15121 catatcaatt ggaatctaat cgcattattt ggtggtatct ttgtcatcaa tgctttatta
    15181 agcggattag gtttatattt attaagtaaa attggtgaaa agattattta tgcgatacgc
    15241 tcagttttat gggagcatat catacaatta aaaatgccat tctttgacaa aaatgaaagt
    15301 ggtcaattaa tgagtcgatt aactgacgat acgaaagtga taaatgaatt tatttcacaa
    15361 aagctaccta acttattacc atcaatcgtt acattagttg ggtcactaat catgttattt
    15421 attttagatt ggaaaatgac attattaaca tttataacga taccgatatt cgttttaatt
    15481 atgattcctc taggtcgtat tatgcaaaag atatcgacaa gtacacaatc tgaaattgca
    15541 aacttcagtg gtttgttagg gcgtgtccta actgaaatgc gtcttgttaa aatatcaaat
    15601 acagagcgtc ttgaattaga taatgcacat aaaaatttga atgaaatata taaattaggt
    15661 ttaaaacagg ctaaaattgc ggcagttgta caaccaattt caggtatagt tatgttgcta
    15721 acaattgcaa ttattttagg ttttggtgca ttagaaattg cgactggtgc aatcactgca
    15781 ggtacattaa ttgcaatgat attttatgtt attcagttat ctatgccttt aatcaatctt
    15841 tccacgttag ttacagatta taaaaaggca gtcggtgcaa gtagtagaat atacgaaatc
    15901 atgcaagaac ctattgaacc gacagaagct cttgaagatt ctgaaaatgt attaattgat
    15961 gacggtgtat tgtcatttga acatgtagac tttaaatatg atgtgaagaa aatattagat
    16021 gatgtgtcgt tccaaatccc acaaggtcaa gtgagtgctt ttgtaggccc ttctgggtct
    16081 ggtaaaagta cgatatttaa tctgatagaa cgtatgtatg aaattgagtc aggtgatatt
    16141 aaatatggcc ttgaaagtgt ctatgatatc ccgttatcta agtggcgacg caaaattgga
    16201 tatgttatgc aatcaaattc gatgatgagt ggtacaatta gagacaatat tttatacgga
    16261 attaatcgtc atgtttcaga tgaagaactt attaattatg ctaaattagc gaactgtcat
    16321 gattttatca tgcaatttga tgaaggatat gacacgcttg taggtgaacg aggattgaaa
    16381 ctgtctggcg gacaacgtca acgtattgat attgctagaa gttttgttaa aaatcctgat
    16441 attttgttac ttgatgaagc aacagctaat ctcgatagtg aaagtgaatt gaaaattcaa
    16501 gaagctttag aaacattgat ggaaggtaga acaacgattg tcattgcgca tcgtttgtct
    16561 acaattaaaa aagccggtca aattatattc ttagacaaag gacaggtaac aggtaaaggt
    16621 acgcattcag aactgatggc atcacatgcg aagtataaaa actttgtagt gtctcaaaaa
    16681 ttaacagatt aattttatat atataagtaa gcttggagca aatacacata taccatcgag
    16741 gaaattaaag tgtggcacat tgatggatat agatgttaat aaattgcttc aagcttttgt
    16801 ctattttaaa tcatttgaga agttacgaca taataattct taaattaatg aaatcgatat
    16861 tttaagaaaa aaatgctcat ggtataatac aagttataag caaacataca tatattaaat
    16921 actgtagcca cgagtcataa ttcttcgtat tttacatagc aatttaactg attttagagt
    16981 ccacggtaca gaagtttgat atttcaatgt ttctaaattt ttaaaaaatt aaatcatagg
    17041 tgggtgccaa atgtttttat taatcaacat tattggtcta attgtatttc ttggtattgc
    17101 ggtattattt tcaagagatc gcaaaaatat ccaatggcaa tcaattggga tcttagttgt
    17161 tttaaacctg tttttagcat ggttctttat ttattttgat tggggtcaaa aagcagtaag
    17221 aggagcagcc aatggtatcg cttgggtagt tcagtcagcg catgctggta caggttttgc
    17281 atttgcaagt ttgacaaatg ttaaaatgat ggatatggct gttgcagcct tattcccaat
    17341 attattaata gtgccattat ttgatatctt aatgtacttt aatattttac cgaaaattat
    17401 tggaggtatt ggttggttac tagctaaagt aacaagacaa cctaaattcg agtcattctt
    17461 tgggatagaa atgatgttct taggaaatac tgaagcatta gccgtatcaa gtgagcaact
    17521 aaaacgtatg aatgaaatgc gtgtattaac aatcgcaatg atgtcaatga gctctgtatc
    17581 gggagctatt gtaggtgcgt atgtacaaat ggtaccagga gaactggtac taacggcaat
    17641 tccactaaat atcgttaacg cgattattgt gtcatgcttg ttgaatccag taagtgttga
    17701 agagaaagaa gatattattt acagtcttaa aaacaatgaa gttgaacgtc aaccattctt
    17761 ctcattcctt ggagattctg tattagcagc aggtaaatta gtattaatca tcatcgcatt
    17821 tgttattagt tttgtagcgt tagctgatct atttgatcgt tttatcaatt tgattacagg
    17881 attgatagca ggatggatag gcataaaagg tagtttcggt ttaaaccaaa ttttaggtgt
    17941 gtttatgtat ccatttgcgc tattactcgg tttaccttat gatgaagcgt ggttggtagc
    18001 acaacaaatg gctaagaaaa ttgttacaaa tgaatttgtt gttatgggtg aaatttctaa
    18061 agatattgca tcttatacac cacaccatcg tgcggttatt acaacattct taatttcatt
    18121 tgcaaacttc tcaacgattg gtatgattat cggtacattg aaaggcattg ttgataaaaa
    18181 gacatcagac tttgtatcta aatatgtacc tatgatgcta ttatcaggta tcctagtttc
    18241 attattaaca gcagctttcg ttggtttatt tgcatggtaa tatgtcgaag agtgactatg
    18301 ataatacatt ttaactaata aatatgtcca ggcatgtcgt ctattgatat aggtgagatg
    18361 cttggacttt tttattattg atataaaggt atttaaatat ttttaaagtt accgaaattg
    18421 aagcattata aaaaccaagt gcacatggta atacacttgg cttttatggg aaatgaatat
    18481 tattgtacat atgacagtaa ggactaggta cagtcatagt acttcgagca aaatttgttt
    18541 tgttattata aacaacacaa aggagataac ttctctattg aagaagttaa aaacattata
    18601 gcagacaatg aaatgaaagt aaattaaaaa ttcagaatat ttttaattat tata
//
LOCUS       DAKTGS010000044        18531 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.044, whole genome shotgun sequence.
ACCESSION   DAKTGS010000044 DAKTGS010000000
VERSION     DAKTGS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.044"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..87
                     /locus_tag="O0286_001270"
     CDS             <1..87
                     /locus_tag="O0286_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCY0169654.1"
                     /translation="CPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            221..1765
                     /gene="miaB"
                     /locus_tag="O0286_001271"
     CDS             221..1765
                     /gene="miaB"
                     /locus_tag="O0286_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCY0169655.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            1766..2131
                     /locus_tag="O0286_001272"
     CDS             1766..2131
                     /locus_tag="O0286_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCY0169656.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            2158..2649
                     /gene="thiW"
                     /locus_tag="O0286_001273"
     CDS             2158..2649
                     /gene="thiW"
                     /locus_tag="O0286_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCY0169657.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            2952..5570
                     /gene="mutS"
                     /locus_tag="O0286_001274"
     CDS             2952..5570
                     /gene="mutS"
                     /locus_tag="O0286_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCY0169658.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            5583..7592
                     /gene="mutL"
                     /locus_tag="O0286_001275"
     CDS             5583..7592
                     /gene="mutL"
                     /locus_tag="O0286_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCY0169659.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            7598..8140
                     /locus_tag="O0286_001276"
     CDS             7598..8140
                     /locus_tag="O0286_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCY0169660.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            8605..9423
                     /locus_tag="O0286_001277"
     CDS             8605..9423
                     /locus_tag="O0286_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCY0169661.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            9552..11048
                     /gene="glpK"
                     /locus_tag="O0286_001278"
     CDS             9552..11048
                     /gene="glpK"
                     /locus_tag="O0286_001278"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCY0169662.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            11206..12879
                     /locus_tag="O0286_001279"
     CDS             11206..12879
                     /locus_tag="O0286_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCY0169663.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            13029..13943
                     /locus_tag="O0286_001280"
     CDS             13029..13943
                     /locus_tag="O0286_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0169664.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            13961..14896
                     /gene="miaA"
                     /locus_tag="O0286_001281"
     CDS             13961..14896
                     /gene="miaA"
                     /locus_tag="O0286_001281"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCY0169665.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            14911..15144
                     /gene="hfq"
                     /locus_tag="O0286_001282"
     CDS             14911..15144
                     /gene="hfq"
                     /locus_tag="O0286_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCY0169666.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(15366..15842)
                     /locus_tag="O0286_001283"
     CDS             complement(15366..15842)
                     /locus_tag="O0286_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0169667.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            15954..17192
                     /gene="hflX"
                     /locus_tag="O0286_001284"
     CDS             15954..17192
                     /gene="hflX"
                     /locus_tag="O0286_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCY0169668.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            17211..18449
                     /locus_tag="O0286_001285"
     CDS             17211..18449
                     /locus_tag="O0286_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0169669.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
BASE COUNT         6892 a         2487 c         3526 g         5626 t
ORIGIN      
        1 tgtccaagta ttattagcca agttaagttt tatcatgtgc cagatggcat cactattgaa
       61 actttaactg aaaaatatga tgaataacat taaaagtgaa gtaaactgga tttgaattgg
      121 cttgttagag atgacgtata actttaactg tttttgcact ttatagttaa atttaatata
      181 attattaaat gatacgggca aatagaaagg attttgtaaa gtgaacgaag aacaaagaaa
      241 agcaagttct gtagatgttt tagctgagag agataagaaa gcagaaaaag attatagtaa
      301 atattttgaa catgtttatc agccgcctaa tttaaaagaa gcgaaaaaaa gaggtaaaca
      361 agaagttcgt tataatagag atttccaaat tgatgaaaaa tatcgcggta tggggaacga
      421 gcgtacattt ttaattaaaa catatggatg tcaaatgaat gcacatgaca ctgaggtcat
      481 tgctggtata cttgaagcat taggctatca agcaacgact gatattaaca ctgcagatgt
      541 tattttaatt aatacatgtg cgattagaga aaatgccgag aacaaagtgt ttagtgaaat
      601 aggtaatttg aagcatttga aaaaagaacg acctgatatt ttaatcggtg tttgtggttg
      661 tatgtcacaa gaagagtcag tagtgaataa aattttaaaa tcgtatcaaa atgtagatat
      721 gatatttggt acacataata ttcatcattt accagaaatt ttagaagaag catacttatc
      781 taaagcaatg gttgttgaag tatggtctaa agaaggagac gttattgaaa atcttccaaa
      841 agtccgtgaa ggcaacatta aagcatgggt caatattatg tatggttgtg ataagttttg
      901 tacatattgt attgttccat ttacaagagg taaagaacga agccgtagac ctgaagacat
      961 tatagatgaa gtacgtgaac ttgctcgtga aggttacaaa gaaataacgc ttttaggtca
     1021 aaatgtaaat tcttatggta aagatttaca ggatatagaa tatgacttag gagatctttt
     1081 acaagcaatt tctaaaatag cgattccaag agttcgtttc acaacaagtc atccttggga
     1141 ctttacagat cacatgattg atgttatttc agagggtggt aatatcgttc cgcatatcca
     1201 cttgccagtt caatctggaa ataatgcagt attaaaaata atgggtagaa aatatacacg
     1261 agaaagttat ttggatttag taaaacgaat caaagataga attcctaatg tagcattaac
     1321 tacagatatt attgtagggt atccaaatga atcagaggaa caatttgaag aaactttaac
     1381 tctgtatgat gaagttggtt ttgaacatgc atatacgtac ttgtattcac aacgtgatgg
     1441 tacgcctgct gctaaaatga aagataatgt acctttaaat gtcaaaaagg aacgattgca
     1501 acgtttgaat aaaaaagttg gtcattattc acaaatagct atgagtaagt acgaaggaca
     1561 aactgtaaca gtactttgtg aaggaagtag taaaaaagat gatcaggttc ttgctggcta
     1621 cactgataaa aataagctag ttaatttcaa agcgcctaaa gaaatgattg gtaaactagt
     1681 ggaagtacga atagatgaag ctaaacagta ttcattaaat ggcagtttta taaaggaagt
     1741 agagccggaa atggtgattc aataaatgta taataaagat gacgtgttga accaagcgga
     1801 taatattgca aataaaatta aaaatttgga tactatcaaa acatatcaac aaattgaagc
     1861 acagattcat cagaaccaaa cgataaagac taaaatggat atgttaaaaa agcatcaaaa
     1921 acaagcagta aactttcaaa attacgggaa acaaaatgcg ctagaacagt cggaacatac
     1981 cattcagagt atagaagcag aaataaatac attgcccata gttgaacagt ttcaaacttc
     2041 acaatatgaa gcgaatcaat tattgaaaat gtttgtatca acaatggaaa cacgtttaaa
     2101 tgaccataat aaagccaagc atagtgatta attacaaaca aaaaggagaa caatcatatg
     2161 aaatcaagaa aactggctat aactgcactt ttaattgcaa taaatgttgt attaagcagt
     2221 attatcatca ttcctctagg accagttaag gcagcaccag tacagcattt tgtaaatgta
     2281 ttaagtgcgg tcatagtagg tccttggtat ggattagctc aagcgcttat atcatcaatt
     2341 ttaagagttc tttttggtac tggtacagct tttgcatttc cgggtagtat gattggagtt
     2401 ttattggcta gtatgtttta catatatcgt aaacatatat ttatggccgc ggtcggtgaa
     2461 gtacttggaa ctggtgtcat cggaagttta atttgtatac cattagcata tttccttggg
     2521 cttcaagact tcttcattaa accgttaatg attacgttca tagtctcaag tgctatcgga
     2581 tctattataa gttatttctt attaattact ctaaaaaaac gtggtattct tcaaaggttt
     2641 ataaaataat tttatgatat atgatttcaa acagagtcat tgttcatttt aatattgaac
     2701 gcccttactt aattaaatgt cttgaatgtt agaattttaa tttaattcta aacatgctat
     2761 tgacacattt tattaggttt gaggcgtttt cttatacatt taagatgctt cgtgattata
     2821 tggtatgaat aggtttgtaa aagtaagtat gttaaaatgt ataagttaaa taattaataa
     2881 ttaaaaagct tacaaacgaa tatattgtaa agacaaattt taaaaagaga caagggttgt
     2941 gaagaaataa tatgtctaat gttacaccaa tgatgcagca atatttaaaa ataaaatcag
     3001 aataccaaga ttgcttatta ttttttagac taggtgattt ctatgaaatg ttttatgaag
     3061 atgccaagga ggcatcacgt gtacttgaaa ttactttaac taaaagagat gctaaaaaag
     3121 aaaatccaat tccgatgtgt ggtgttccgt atcattctgc agatagttat atagatacac
     3181 ttgttaataa tggatataaa gtagctattt gtgaacagat ggaagatccg aaacaaacga
     3241 aaggtatggt tagacgtgag gtagtaagaa ttgtgactcc aggaactgtg atggagcaag
     3301 gtggtgtaga tgataaacaa aataactata ttttaagttt tgttatgaat caacctgaaa
     3361 ttgcgcttag ttactgtgat gtttctactg gcgaattaaa ggttacacat tttaatgatg
     3421 aagcgacttt attaaatgaa attacgacga taaaccctaa cgaagttgtt atcaatgaca
     3481 atatttccga taatttaaaa agacaaatta atatggtgac agaaacaata acagtcaggg
     3541 aaacgttatc atcagaaatc tatagtgtga atcaaactga acataaatta atgtatcaag
     3601 cgacacaatt attgctagat tatattcatc atacacaaaa acgtgattta tcgcatatcg
     3661 aggatgttgt tcaatatgca gctatagatt atatgaaaat ggatttttat gctaagagaa
     3721 accttgagtt aacggaaagc attcgattaa aatcaaaaaa aggaacgcta ctttggctaa
     3781 tggacgaaac gaaaacacca atgggagcac gccgcttaaa acaatggata gatagaccac
     3841 taataagtaa agaacaaatt gaagcacgat tagatatcgt tgatgaattt agtgctcatt
     3901 tcatagaaag agacacctta agaacatatc ttaatcaagt gtatgatatt gaacgtcttg
     3961 ttgggcgtgt tagttacgga aatgttaatg cgagagattt aattcaactt aaacattcca
     4021 tttctgaaat accgaatatt aaagcattac taaattctat gaatcagaat actcttgtac
     4081 aagttaatca actagaaccc cttgatgatt tacttgatat attagaacag agtttagtag
     4141 aagaaccacc aatttcagtt aaagatggcg gactattcaa agttggtttt aatacgcaat
     4201 tagatgaata tcttgaagct tcaaaaaacg gaaaaacatg gttagcagaa ttacaagcca
     4261 aagaaagaca acgtacagga ataaaatcat tgaaaataag ctttaataaa gtgtttggtt
     4321 attttataga aataacacgt gccaacttgc aaaattttga accaagtgaa tttggttata
     4381 tgaggaagca aacgttatcg aatgctgaac gttttataac tgatgaactt aaagaaaaag
     4441 aagatatcat tttaggtgcg gaagacaaag ccatcgaatt agaatatcaa ttatttgttc
     4501 agctacgtga agaagttaaa aaatatactg aacgtttaca acaacaagct aaaattattt
     4561 cagagctaga ttgtttacag agctttgcag aaattgctca aaaatataat tacactaggc
     4621 cttcatttag tgaaaataaa acattagaat tagtggaatc taggcaccca gtagtggaaa
     4681 gagtaatgga ttataatgac tatgtgccta ataattgtcg attagataat gaaacattta
     4741 tatatttaat tacaggtccg aatatgtctg gtaaatcgac atatatgaga caagttgcca
     4801 taattagtat aatggcccaa atgggagctt atgtcccttg taaagaggca gtgttaccta
     4861 tatttgatca aatattcact agaataggtg cggcagatga tttggtttca ggtaagagta
     4921 cgtttatggt agaaatgcta gaagcacaaa aggcattaac ttatgcaaca gaggatagtt
     4981 tgattatttt cgatgaaatt ggacgtggta cttcaacgta tgacggttta gctttagcgc
     5041 aggcaatgat agagtatgta gctgaaacat cacatgctaa aacgttattt tcaacacatt
     5101 atcatgaatt gacaacatta gatcaagcat taccaagtct aaaaaatgtt cacgtcgctg
     5161 ctaatgaata taaaggtgaa cttatattct tgcataaagt caaagatggt gcagttgacg
     5221 atagttatgg tattcaagtt gcgaaattag ctgatttacc tgaaaaagtt attagcagag
     5281 cacaagtgat tctaagcgag tttgaagcgt ctgctggtaa aaaatcatcg atatcaaatt
     5341 taaaaatggt cgaaaatgaa cctgaaatta atcaagaaaa tttaaactta agtgttgaag
     5401 aaacaactga tactttatct caaaaagact ttgaacaagc atcatttgat ttgtttgaaa
     5461 atgatcaaga aagcgagatt gaactacaaa ttaaaaattt gaatttatct aatatgacac
     5521 caattgaggc attggtgaag ttaagtgaat tacaaaatca attaaaatag aggtgttgca
     5581 aaatggggaa aattaaagaa ctccaaacct cattagcaaa taaaatcgca gcaggtgaag
     5641 tagttgaaag accgagttct gttgtgaaag aactgttgga aaatgctata gatgcaggcg
     5701 ctacagaaat aagcattgaa gtagaggaat ctggcgtcca atctattcgc gtagtcgata
     5761 atggaagcgg aattgaagcg gaagacttag gattagtatt tcatagacat gcgactagta
     5821 aattagatca agatgaagat ttatttcata ttaggacatt aggattccgt ggtgaagcac
     5881 tagccagtat ttcatcagtt gctaaagtaa cattgaagac ttgcacggat aatgctaatg
     5941 gaaatgaaat atatgtagaa aatggtgaaa tattaaatca taagcctgca aaagcgaaaa
     6001 aaggaacaga tatacttgta gaatcattat tttataatac accagcacgt ttaaaatata
     6061 ttaaaagttt atacactgaa ctaggtaaaa taacagatat tgtcaacaga atggcaatga
     6121 gccatccgga cattcgaata gcactcattt cagatggcaa aacaatgtta agtacaaatg
     6181 gttcaggacg aactaatgaa gtgatggcag agatttatgg gatgaaagtt gcacgagatt
     6241 tagtacatat atctggagat acaagtgatt atcacattga aggttttgtt gcaaagcctg
     6301 aacattctag aagtaataag cactatattt ctatttttat taatggacga tacattaaaa
     6361 actttatgct aaataaagcg attttagaag gctatcatac actcttaaca ataggtaggt
     6421 tcccgatttg ttatattaat attgaaatgg atccaatctt agtagacgta aatgttcatc
     6481 caacaaaact agaagtgcgt ttatcaaaag aagagcaact ataccaattg attgtgagca
     6541 aaatacaaga agcatttaaa gaccgtatat taattcctaa aaataacttg gattatgtgc
     6601 caaaaaaaaa taaagtatta cattcattcg aacaacaaaa aatcgaattt gaacaaagac
     6661 aaaacacaga gaataaccaa gagaagacgt tttcatctga agaaagtaac agtaagccat
     6721 ttatggaaga aaatcaaaac gatgagatag ttataaaaga agattcatat aatccattcg
     6781 taacgaaaac atctgaaagt ttaatagctg atgatgaatc ttctggttat aataatacac
     6841 gtgaaaaaga tgaagactac ttcaaaaaac aacaagaaat tttacaagaa atggatcaaa
     6901 catttgattc aaatgatggt acaactgtgc aaaattatga gaataaagcg tctgatgatt
     6961 attatgatgt aaacgatatt aaaggaacaa aaagtaaaga ccctaaacga agaattccat
     7021 atatggaaat tgttggacaa gtacatggaa cgtatattat tgctcaaaat gaatttggca
     7081 tgtacatgat tgaccagcat gcagctcaag aaagaataaa atatgaatat tttcgagata
     7141 aaataggtga ggttaccaat gaagtacaag atttattaat cccgttaaca tttcattttt
     7201 caaaagatga acaattagtc attgatcaat ataaaaatga gcttcaacaa gtaggtatca
     7261 tgttagaaca ttttggtggt catgattata ttgtaagtag ctatccagtt tggttcccta
     7321 aagatgaagt agaagaaatt attaaagata tgattgagct aattttggaa gagaaaaaag
     7381 tagatatcaa aaaattacgt gaagatgtag caatcatgat gtcatgtaaa aaatccatta
     7441 aagcgaatca ttatttacaa aaacatgaaa tgtctgattt aattgatcaa ttaagagaag
     7501 cggaagatcc atttacatgt ccacatggtc gtccaataat cattaatttt tcaaaatacg
     7561 aattagaaaa attatttaag cgtgtgatgt agagaggatg aatcaagtga ataacaacat
     7621 attgcctgct ataagaaaca ttaaagattt agagaaactg attaaaacag actataaaat
     7681 gtgtgtgctt ctagatatgc atataggaca tataaaaagt attatggaat tgctgaagca
     7741 aaatcatata gagtgtttta ttcatataga tttgataaaa ggtttaagcc acgatgaatt
     7801 tgcaagtgaa tttattattc agcaatacaa gccaaaaggt atcgtatcga ctaaatctaa
     7861 agtaataaaa aaagctaaat cattaaatac tttaacgatt tttagagtat ttattattga
     7921 tagtcaagca ttgaaacgca gtatagattt gataaaaaaa gttgaacctg attttgttga
     7981 agtacttcca ggtgttgcga gtaaagcgat tcatcatatt cagaaagaaa caaacacaca
     8041 agtcattgca ggtggcctaa ttaatacaat agatgaagtc aatgaagctg ttaaaaatgg
     8101 agcgaaatat gtaactacta gttatgataa actttggtaa atcataagtc tgcaaaaatt
     8161 tcatttttca cgagatttag tcatataaca taataaaaaa gccgttttca tatgggatat
     8221 gcagtaggta tcaaacaaaa aataatcaaa taaaataatt agcacctcta aaacgattaa
     8281 aatcattaaa gttttagagg tgtttattag taaaaaatgc aactagtcat aaaaaatatg
     8341 gctgaagaat gtcaattaaa caaaagagcg tcataaataa attagttagg attggtattt
     8401 ctagttttca aattaaacta cttttataaa gacttgaaaa attaaatttg acaaaatttt
     8461 tacaaaaacc attgacaacg ctttcatatc gatagtatca taacaaatat aataaaagtt
     8521 aatacataga atagagacgg gagatttcta cgagccaaac tgctagtgta ggaatctctt
     8581 tgtctttttg ggaggacatt taatatgaat gtatatttag cagaattcct aggaactgca
     8641 atcttaatcc tttttggtgg tggcgtttgt gccaatgtca atttaaagag aagtgctgcg
     8701 aatggtgctg attggattgt catcacagct ggatggggat tagcggttac aatgggtgtg
     8761 tttgctgtcg gtcaattctc aggtgcacat ttaaacccag cggtgtcttt agctcttgca
     8821 ttagacggaa gttttgattg gtcattagtt cctggttata ttgttgctca aatgttaggt
     8881 gcaattgtcg gagcaacaat tgtatggtta atgtacttgc cacattggaa agcgacagaa
     8941 gaagctggcg cgaaattagg tgttttctct acagcaccgg ctattaagaa ttactttgcc
     9001 aactttttaa gtgagattat cggaacaatg gcattaactt taggtatttt atttatcggt
     9061 gtaaacaaaa ttgccgatgg tttaaatcct ttaattgtcg gagcattaat tgttgcaatc
     9121 ggattaagtt taggcggtgc tactggttat gcaatcaacc cagcacgtga tttaggtccg
     9181 agaattgcac atgcgatttt accaatagct ggtaaaggtg gttcaaattg gtcatatgca
     9241 atcgttccta tcttaggacc aattgccggt ggtttattag gtgcagtggt atacgctgta
     9301 ttttataaac atacatttaa tattggttgt gcaattgcaa ttgttgtagt tattattact
     9361 ttgattttag gttacatttt aaataaatca tcaaaaaaag gtgatatcga atcaatttac
     9421 taaaataaaa agaaacgtaa atagcataat ttaacatgtt tgattcatgg attatgctat
     9481 tttttcgcca aaatttaaca gattttgtac aatgggttag cgattatttt ttaataaagg
     9541 agatactact aatggaaaaa tatattttat ctatagacca aggaacaaca agctcaagag
     9601 cgattttatt caatcaaaaa ggggaaattg caggggtagc acaacgtgag tttaagcaat
     9661 attttccaca atcaggttgg gttgaacatg atgcaaatga aatttggaca tctgtgttag
     9721 ctgtaatgac ggaagtaatt aatgaaaatg atgttagagc tgatcaaatt gcaggtatcg
     9781 gtattacaaa ccaacgtgaa acaacggttg tttgggacaa acatactggc cgcccaattt
     9841 atcacgcaat tgtttggcaa tcacgtcaaa cacaatcaat ttgttcagaa ttaaaacaac
     9901 aaggatatga acaaacattt agagataaga caggattact tttagatccg tattttgcag
     9961 gtacaaaagt taaatggatt ctagacaatg ttgaaggtgc acgagaaaaa gcagaaaatg
    10021 gcgatctatt atttggaacg attgatactt ggttagtatg gaaattatca ggaaaagctg
    10081 cgcatattac tgattattca aatgcgagtc gtacattaat gtttaatatc catgatttag
    10141 aatgggacga tgagttatta gaactactta cagtacctaa aaatatgttg ccagaagtta
    10201 aagcttcgag tgaagtatat ggtaagacaa ttgattacca cttctatggt caagaagtac
    10261 caatcgctgg agtagctggt gatcaacaag cagcattatt tggacaagct tgcttcgaac
    10321 gtggtgacgt gaaaaacaca tatggaactg gtggcttcat gttaatgaat acaggtgaca
    10381 aagcggttaa atctgaaagt ggtttattaa caacaattgc ttatggtatt gatggaaaag
    10441 taaattatgc gcttgaaggt tccatctttg tttcgggttc agcaatccaa tggttacgtg
    10501 atggattaag aatgattaat tcagcaccac aatcagaaag ttatgcgaca cgagttgact
    10561 ctactgaggg tgtttatgtt gttccagctt ttgtaggttt aggaacacca tattgggatt
    10621 ctgaagcacg tggtgcgatt ttcggtttaa cacgtggaac tgaaaaagag cactttatcc
    10681 gtgcaacttt agaatcacta tgttaccaaa ctcgtgacgt tatggaagca atgtcaaaag
    10741 actctggtat tgatgtccaa agtttacgtg tcgatggtgg tgcagttaaa aataacttta
    10801 ttatgcagtt ccaagcagac attgttaata cttctgttga aagacctgaa attcaagaaa
    10861 ctacagcttt aggtgctgca tttttagcag gtttagcagt tggattctgg gagagtaaag
    10921 atgatatcgc taaaaactgg aaattagaag aaaaattcga tccgaaaatg gatgaaggcg
    10981 aaagagaaaa attatataga ggttggaaaa aagctgttga agcaacacaa gtttttaaaa
    11041 cagaataaac ttgtagatta gacttttgta taaacattgt gatacaatca atttaagtta
    11101 atatttgaat cgagaagcga gagatttgtt cgaacatgta caattgaagg attgtttatg
    11161 tgggacaagt ctctcgtttt tacatttttt aggaggcgtt ttggaatggc attgtctact
    11221 tttaagagag aacatattaa aaagaattta agaaatgatg aatatgattt agtaattatt
    11281 ggtggcggta ttacaggtgc aggtattgca ctagacgcga gtgaaagagg aatgaaagtt
    11341 gcattagttg aaatgcaaga ctttgcacaa ggaacaagct caagatctac aaaattagtc
    11401 catggtggtt tgcgttactt aaaacaattc caaattggag tagttgccga aactggtaaa
    11461 gaacgtgcga ttgtttatga aaatgggcct catgttacga ctccagagtg gatgctttta
    11521 ccaatgcata aaggtggaac atttggtaaa ttctcaacat caattggttt aggaatgtat
    11581 gatcgtttag caggtgttaa gaagtctgaa cgtaaaaaaa tgttatctaa aaaagaaact
    11641 ttagctaaag aaccattagt taaaaaagaa ggtctaaaag gcggcggtta ctatgttgaa
    11701 tatcgtactg acgatgcgcg tttaactatt gaagttatga agcgtgctgc tgaaaaaggc
    11761 gcagaaatta tcaactatac taaatctgaa cacttcactt atgataaaaa tcaacaagta
    11821 aatggtgtta aagttataga taaattaact aatgaaaatt atacaattaa ggctaaaaaa
    11881 gtggttaatg cagcaggtcc atgggttgat gatgttagaa gtggtgatta tgcacgcaat
    11941 aataaaaaat tacgtttaac taaaggtgta catgttgtta ttgatcaatc aaaattccca
    12001 ttaggtcaag cagtatactt tgatactgaa aaagatggaa gaatgatttt tgcaattcca
    12061 cgtgaaggaa aagcgtatgt aggtactaca gatacattct atgacaatat caaatcttca
    12121 ccattaacta cacaagaaga cagagactat ttaatcgatg cgattaatta catgttccct
    12181 agtgttaatg ttacagatga agatattgaa tcaacatggg caggaattag accattaatt
    12241 tacgaagaag gcaaagaccc ttctgaaatc tctcgtaagg atgaaatttg ggaaggtaaa
    12301 tcaggtttat taactattgc aggtggtaaa ttaacaggct atcgtcacat ggctcaagac
    12361 attgttgatt tagtatctaa acgcttgaaa aaagactacg gtttaacatt tagtccatgt
    12421 aatacaaaag gtctggcaat ttcaggtggc gatgtaggtg gtagcaagaa ctttgatgcg
    12481 tttgtagagc aaaaagtaga tgtagctaaa ggattcggca ttgatgaaga tgttgcaaga
    12541 cgtttagcat ctaaatatgg ttcaaatgtt gatgaattgt tcaacattgc gcaaacatct
    12601 caataccatg atagcaagtt accattagaa atttatgtag aacttgttta tagtattcaa
    12661 caagaaatgg tatacaaacc taacgatttc ttagttcgtc gttctggtaa aatgtatttc
    12721 aatattaaag atgtattaga ttataaagat gctgtcatcg atattatggc agatatgctt
    12781 gattactctc cagctcaaat tgaagcatat actgaagaag ttgagcaagc aattaaagaa
    12841 gcgcaacatg gaaataatca accagcagtt aaagaataat taatttgtac aatcataaac
    12901 tggtgtcctg ttttaagggc atcagttttt ttatacgaga tacattagtc aattgaaact
    12961 atgcatcact aaatgtatga taataataaa agcgaatata aaagcgttgt tattaaaggt
    13021 gtgagatgat gactgaaaaa caatttaaat taactgtaca agataatacg aatattgaag
    13081 ttaaagtgaa ttttacagat gtagattcaa aaggaattat tcatatattt catggtatgg
    13141 ctgaacatat ggaacgttac gataaattag cacatgcact ttcaaagcat ggcttcgatg
    13201 tgatacgtca taatcatcga ggacatggta ttaatattga tgaatcaaca agagggcatt
    13261 acgatgatat gaaacgagtt atcggtgatg cctttgaagt agcgcaaaca gtgagaggca
    13321 atgttgataa accatacatt ataatcggac attcaatggg atccgttata gctagattgt
    13381 ttgtagaaac atatccgcaa tatgttgatg gtctaatttt aagtggtact ggtatgtatt
    13441 cattatggaa aggtttacca accgttaaag tgttacaact gattacaaaa atttatggtg
    13501 ctgagaaacg agttgaatgg gttaaccagt tagtatcaaa tagttttaat aaaaaaatac
    13561 gtccattacg tacacaaagt gattggattt ctagtaatcc aattgaagta gataacttta
    13621 ttaaagatcc atatagtgga tttaatgtgt caaatcaatt attatatcaa acagcctatt
    13681 atatgctaca tacatcacaa ttaaaaaata tgaaaatgtt aaatcatgcc atgcctatat
    13741 tattagtttc aggatatgac gatcctttag gtgattatgg taaagggatt ttaaaattgg
    13801 cgaatatata tagaaaagct ggcattaaaa atgttaaagt gaatctttat catcataaac
    13861 gtcatgaagt gttatttgaa aaagatcatg acaaaatttg ggaagacttg tttaaatggt
    13921 tgaatcaatt ttataaaaaa taaagaaagt ggaattaaat atgaataaaa ataagccttt
    13981 tattgtagta attgtggggc caactgcttc aggtaaaaca gagcttagca tcgaactcgc
    14041 gaagcgtatc aatggtgaaa tcataagcgg tgactctatg caagtctaca aacatatgaa
    14101 tattggaact gcaaaagtaa cacctgaaga aatggatggt attccacatc atttaattga
    14161 tatcttgaat cctgatgata cattttcagc atatgaattc aagcgattag cagaagattt
    14221 aattactgat ataacgaata gaggtaaagt tccaatcata gcaggtggaa caggcttata
    14281 tattcaatca ttaatatata attatgaatt agaagatgaa acagttacac ctgcacaatt
    14341 atccatagtt aaacaaaagt tatctgcatt agaacattta gataatcagc aactacacga
    14401 ttatttagct caatttgatg cggtttctgc agaaaatatt caccctaaca accgccaaag
    14461 agtgttgcgc gctattgaat attatttaaa aacaaaaaaa cttttgagta atcgcaagaa
    14521 agtgcaacaa tttactgaaa attatgatac attattatta gggattgaaa tgtcgcgtaa
    14581 aacattatat tcaagaataa ataaacgtgt tgatattatg ttggatcacg gattatttag
    14641 agaagtgcaa caacttgttg aacaaggcta tgaatcttgc caaagtatgc aagctattgg
    14701 atataaagaa ttaatacctg tgattaacgg acaaatgatt tatgaagatg ctgtcaatga
    14761 tttaaagcaa cattcacgcc aatatgcaaa acgacaaatg acatggttca agaataaaat
    14821 gagtgttcat tggttagata aagaaaatat gtcacttcaa atgatgttag atgagattac
    14881 aacccagatt aagtaaaagg agtccgacag atgattgcaa acgaaaacat ccaagacaaa
    14941 gcactagaga attttaaagc aaaccaaact gaagtaactg tattctttct aaacggtttc
    15001 caaatgaaag gtgttattga agaatacgac aagtatgtcg taagcttaaa ttctcaaggc
    15061 aaacaacact tgatttacaa acatgcgatc agcacttata cagtagaaac tgaaggtcaa
    15121 gcatctactg aaagtgaaga ataagttgta atcatatggg cacgatttaa tgactcgtgt
    15181 acataacgtt tgaagtatga taaatagata tagaaataaa gtcatatgtg acatcaatta
    15241 aatgatgttc aaatgacaag atacaataga ggaatgtttg taataaaaac gcttcatata
    15301 aaggtcgagt caatatatga tacgacttta tatgaagcgt tttatttgct atgagctagt
    15361 atattttata ataatttttc tatttctctt tcgatttgaa caggtttttt ttgaggtgca
    15421 aatcgtttaa caacgttacc ttcgcgatcc actaaaaact tagtgaaatt ccatttgatt
    15481 ttctcattaa agaatccgtg ttgtgccgca gtcaaatatc taaataaagg taattgatgt
    15541 tcccctttta cgtctatttt ttgatgcata gggaaggtaa caccatagtt taatttacag
    15601 ttttgagctg cttcttcgcc tgaaccaggt tcttggccac caaattgatt acaagggaaa
    15661 cctagaatta caaacccttg atctttgtat ttctcgtata atgattgcaa accttcaaat
    15721 tgtgaagtaa agccacattc gctagctgta ttaacaatta gcataacgtc acccttatat
    15781 gcatctaatt tgtaagtaac acctttattt gtttctacta caaaatcata aattgtctcc
    15841 attgtatcat cctttcgatt tacttaaaat gtaccacaaa atcgtgtaat agtctttact
    15901 aaaactctat gatagaatac tttgagtagg attttattaa ggagatgtat aacatggctc
    15961 agcaacaaat tcatgatact aaaaataaac tagaaaaagc tgtcttagtc ggtgtacatg
    16021 ctcaagatga taagcaattt aattttgagt ctacaatgga ggaattatca tctttatcag
    16081 agacttgcca acttgaagtg ttgggtcaaa ttactcaaaa cagagatcgt gtagatcgca
    16141 aatattatgt tggtaaaggt aaaattgaag aaattcaagc atttattgag ttcaaagata
    16201 ttgatgtagt catcacaaat gatgaattaa cgactgcaca atccaaatca ctaaatgaag
    16261 ctttaggtgt aaaaattatt gatagaactc agttgattct tgaaatattt gcattaagag
    16321 caagaagtaa agaaggtaaa ttgcaagtag agctagcaca acttgattat ttattaccta
    16381 gattgcaagg ccatggtaaa agcctttctc gtttaggtgg cggtattgga actagaggcc
    16441 ctggtgaaac gaagttagag atggatcgca gacatattcg aactcgtatg aatgaaatta
    16501 aacatcaatt gcggacggta gaagaacatc gcgaaagata tcgaaataaa agaaatcaaa
    16561 atcaggtgtt tcaagtagct ttagttggtt atacaaatgc tggtaaatca tcatggttta
    16621 atgttttagc aaatgaagag acgtatgaaa aagatcaatt atttgcaacg ttagatccta
    16681 aaacacgaca aattcaaata aatgatggat ttaatttaat tatttcagat actgttggtt
    16741 ttatacagaa actacctacg acgttaattg cagcttttaa atcaacttta gaagaggcta
    16801 aaggtgcaga tttattagta catgtcgtag atagtagcca tcctgaatac cgtacgcagt
    16861 atgacacagt taatgattta atcaaacaat tagatatgag tcatatttct caaatagtta
    16921 tttttaataa aaaggactta tgtgatcatg catcaaatcg tccagcaagt gatttgccta
    16981 atgtttttgt ttcttctaaa aatgatggtg ataaattact tgttaagacg ttatttattg
    17041 atgaaatcaa aaggcaatta acttattatg atgagacaat tgcgacgaat aatgcagatc
    17101 gattatattt tctaaaacaa catacattag tgactgaact taaatatgat gaaattgaaa
    17161 atgtttatcg tataaaagga tttaaaaaat aataaaagga cgaaattcaa atgaaagata
    17221 taagtaagat agtagctgac gtcgaatcaa cgttagcacc atattttaaa gaaattgaag
    17281 aaacagcata tattaatcaa gaaaaagtat taaatgcatt tcatcatgtc aaagcaaccg
    17341 aaagtgatct acaaggatca acaggatacg ggtatgatga ctttggacgt gatcatttag
    17401 aagaaatata tgcgcaggca tttaaagcag aagatgcaat tgttcgtccg caaattattt
    17461 caggtacgca tgcgattact attgcattac aaagtttatt aaaacatggt gatgaattaa
    17521 tttatataac gggtagtcca tatgacactt tacttgaagt cattggcgta aacggaaatg
    17581 gtattgaaag tttaatggag catggcgtat cgtataaaga tattgcactt aaagaaggta
    17641 agatcgatat tgaaagtgtg ttagatgggg tttctgagcg caccaaagta atagcgattc
    17701 aacgttcgaa aggctatgat caaagacctt caattccgct agatgaaatt gaaaaggtaa
    17761 ttactaggtt gaaaaacgtg catcctaata ttttaatatt tgtggataac tgttatgggg
    17821 aatttgttga aagacgtgaa cctatagaat gtggtgccga tttaatagca ggatcattaa
    17881 ttaaaaaccc tggcggtggt ttagctaaga ttggtggata cattgctggt agaaaagatt
    17941 taattgaacg atgtggttat agattgacag cacctggtat tggtaaagaa gcgggtgcat
    18001 cattaaatgc attgcttgaa atgtatcaag gtttcttttt agcaccacac gttgtcagtc
    18061 agagtcttaa aggtgcattg tttactagtt tatttttaga aaaaatgaat atgaacacaa
    18121 cgccgaagta ctacgaaaaa cgaactgatt taattcaaac agttaaattt gaaacgaaag
    18181 aacaaatgat ttcattttgt caaagtattc aacacgcatc cccaattaat gcacatttta
    18241 gtccagaacc tagttatatg cctggttacg aagatgatgt tattatggca gctggtacgt
    18301 ttattcaagg ttcatcgatt gaattatctg cagatggacc tattcgtcct ccttatgaag
    18361 catatgttca aggaggatta acatatgaac acgttaaaat tgctgtgaca agagctgtta
    18421 atcagttgaa agaacaagga cttatataat agattaaatt tcaccctaaa tgataatgtg
    18481 ttttgggtga aattttttgc ggagatttat cagattattt ttaaaaattg a
//
LOCUS       DAKTGS010000045        18410 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.045, whole genome shotgun sequence.
ACCESSION   DAKTGS010000045 DAKTGS010000000
VERSION     DAKTGS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.045"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(72..764)
                     /locus_tag="O0286_001286"
     CDS             complement(72..764)
                     /locus_tag="O0286_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCY0169670.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1149..1946
                     /locus_tag="O0286_001287"
     CDS             1149..1946
                     /locus_tag="O0286_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0169671.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2079..3026
                     /locus_tag="O0286_001288"
     CDS             2079..3026
                     /locus_tag="O0286_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0169672.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            3628..6582
                     /gene="fdhF"
                     /locus_tag="O0286_001289"
     CDS             3628..6582
                     /gene="fdhF"
                     /locus_tag="O0286_001289"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCY0169673.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            6582..7055
                     /locus_tag="O0286_001290"
     CDS             6582..7055
                     /locus_tag="O0286_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCY0169674.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            7283..7516
                     /locus_tag="O0286_001291"
     CDS             7283..7516
                     /locus_tag="O0286_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169675.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            7538..8314
                     /locus_tag="O0286_001292"
     CDS             7538..8314
                     /locus_tag="O0286_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0169676.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            8625..9749
                     /locus_tag="O0286_001293"
     CDS             8625..9749
                     /locus_tag="O0286_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCY0169677.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(9841..10794)
                     /locus_tag="O0286_001294"
     CDS             complement(9841..10794)
                     /locus_tag="O0286_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCY0169678.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(11156..11656)
                     /locus_tag="O0286_001295"
     CDS             complement(11156..11656)
                     /locus_tag="O0286_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0169679.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(11893..12315)
                     /locus_tag="O0286_001296"
     CDS             complement(11893..12315)
                     /locus_tag="O0286_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCY0169680.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYIIPLTIRFI"
     gene            12563..13645
                     /locus_tag="O0286_001297"
     CDS             12563..13645
                     /locus_tag="O0286_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCY0169681.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            13740..15140
                     /gene="nhaC"
                     /locus_tag="O0286_001298"
     CDS             13740..15140
                     /gene="nhaC"
                     /locus_tag="O0286_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCY0169682.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            15314..15556
                     /locus_tag="O0286_001299"
     CDS             15314..15556
                     /locus_tag="O0286_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169683.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(15573..16376)
                     /locus_tag="O0286_001300"
     CDS             complement(15573..16376)
                     /locus_tag="O0286_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0169684.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            16986..>18410
                     /locus_tag="O0286_001301"
     CDS             16986..>18410
                     /locus_tag="O0286_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0169685.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLI"
BASE COUNT         6458 a         2509 c         3429 g         6014 t
ORIGIN      
        1 tacgaagtat tgtataaata gagaacagca gcaagatatt ttctaattga aaatcatctt
       61 actgctgttt tttaggaatc gatggggttt aaaaattgat gatagttatc aatggatatg
      121 tctctggttt gaatcgtctt accgaatgaa tacgaaatat ctataatatt ttcaatttcg
      181 agttcggcag ttttgtcttg agcaatgtca taaatgactg catgccaatg tccatgttta
      241 cacaaaaggc caataaatat aacgttttca gctttaatat tatcatcaac cctatgatct
      301 actaacatca catttcgttc tgtacaataa attaaaatgt cggaaaacag cattgggagt
      361 gtcatatgtt gtgtgtcatc aaatttaata taaaagtcca tgcgtttaag caattttctt
      421 aagcgtgttt gtggaagact taaacattgt tttataattg cgttgatttc gcttttatat
      481 ggtaatgtcg agtacgactg actttcatta agcactaaaa ataaggctga taattgtgtt
      541 tcagataagt ttaatgcaat tttagattgt gcttgttgta tttggtaacc accatttttc
      601 ccataatgcg cataaatttt aacaccttga ttttctaaat catcaatatc tcttaaaatt
      661 gtgcgtttgg atacgttgca atatttagct aattctaaag cggtcatttt tttattttgt
      721 tgaattgcag taattattaa attttgcctt tcagctttat tcatgacagc cacctcctaa
      781 aagatgatat attacacaaa gtataacata agagtgacaa tgaggacatt ttcaatatct
      841 tttaaaaaaa atcgtaaaaa ttttgtggaa aacgcttaca ttttaaaaaa gattgtgcta
      901 tgatattact tgtaacaggg ggagcgatta aacaaagggg tagagctata taacagatag
      961 cttttaatcg ttcaagttac atcacaacat cttcaatatt tattacttac tttcctttct
     1021 atttgtcggc tagcacgtga ctagccgatt tttttatgca taaaatgaag aagtcttcag
     1081 ttgtattgtt gattctttta atatagggta ataataaaat gataatgata tgagaagtgg
     1141 tggacgaaat gacagataaa acattacagc aaatagataa gttaatatgt tcgtggttga
     1201 aacaaataga caatgttatt ccgcaattaa ttatggaaat gactacagaa actaagcgtc
     1261 atcgatttga tttagttaca aatgtagata aacagattca acaacaattc caacaatttt
     1321 tagcaacaca ttttccggaa caccaactat tagcggaaga aaaaagtaat gaaatgatta
     1381 cgaatgaaat taatcattta tggattatgg atcccattga tggaactgcc aatttagtaa
     1441 aacagcaaga agattattgt attatattgg cgtattttta tgaaggtaaa ccaatgttat
     1501 cgtacgtata tgattatccg cataaaaagc tttataaagc aatacgagga gaaggtgctt
     1561 tttgtaatgg aattaagatg gaagaaccac catcgttaaa attggaagac gctattatat
     1621 cgtttaatgc acaagtgatg aatctagata cggtgcaaga tttatttgac gcctcattta
     1681 gttatcgttt agttggtgct tgcggattag attctatgcg agtggcaaaa ggtcaatttg
     1741 gggcacatat taatacaaat cctaaacctt gggacattgc tgcacagttt ttatttgcag
     1801 aactattgaa tctaaaaatg acgactttag atggtaaggc aattgatcat ttaaagggtg
     1861 caccttttat tataagtaat aaagcatgtc atgaaacggt acttaaaatt ttaaatgcaa
     1921 atggtggtta tcaaaaatac agatagatat tctctagaga taaaaaatga taaaaaatag
     1981 ttatacgtct acagaagtat agtaaaaaaa ttattttact atataataaa tagagaaaac
     2041 agacaaagta tgtaaggaat agtaaaggag tggtgggatt gaaacgttcg tctaaaagta
     2101 aaatgagcct accgaaaaaa atatttttat gggtattcgg tattttagta atcttagcga
     2161 ttgtagctgt tgtgtatgta gctgctaaaa tttttattac tggtaataag atacacaatc
     2221 cgttagatcg caatcattca gaattaagag ataaaaaagt gagtttaaac gatggtgatc
     2281 cattcacaat cgctttattt ggtgtcgatt cagatgctga tcgtaagaaa aagggtggcg
     2341 gagagcgtag tgatagtatt atgattttat ctatcaaccc taaaacgaag aaaactgaaa
     2401 tagtaagtat accgcgtgat acaagagcgg aaattgttgg ccgtggtact actgaaaaaa
     2461 ttgcacatgc ctatgcatat ggtggaccaa atatggctgt taaatcactt gaaaaattaa
     2521 tgaatgtgcc aattgatcat tatgcgacaa tcgatatgga tggtttgcat aatatgatag
     2581 atagcatcgg cggtgttgat gtagtaagta atgatacatt cactgttgat ggtgttcgtt
     2641 ttacaaaagg acagcagaca catgtaaatg gtgatcaagc attgaagttt attagaagtc
     2701 gtaaagaaga aggtgctggt ggtgactttg gtcgacaaca acgtcagcaa attgtgcttg
     2761 aagcgatggc caataagatt gcaagcccat catcgattac acattttaat agtctaatga
     2821 atgaaattca aaataatgtt aaaacagatt taacattagg tgatttgaat acgattagaa
     2881 gcaattataa agatgctaat gacacgatta ataaacatca attgagtggt caaggcggca
     2941 ttcaaagtga cggattgtac tacttcattc ctagtgagca gtcaaaagct gaaagtacta
     3001 agttattaaa agataatcta gagtagtaaa ctaagggaaa ataagttatc aaaattgtac
     3061 acaaattaat tagatagaat gatctgtaaa tagaataacg aatagaacta atagcccgta
     3121 aatagaaggt gataacactt tgtatttatg ggctatttta tttaatgcct gatttgattt
     3181 ttataaatta atccattaca aatttaattt aaagataaat attaccttct aaatgatagt
     3241 agatatttat caaacttggg ttattaattt tcagaaaaag ggtatttata aatagtataa
     3301 cgatttttag gtgaggtgat tattaaatat tgtcattaat aaggttgtga aagatatggc
     3361 aaaaagcgtg aaacagataa ttggaaaact aataatgata attattttca attaaacaaa
     3421 aagatgattt aatttcgcgc aaaatatttc aaataaaaat gattgtaaaa aggcaaaata
     3481 caaaatttta cttaacaact agtacataaa gtaatacaat taaattaatt ctatctgaaa
     3541 gatgtgtggg gcatcgttat tttaggtgga tatgagcaat ttattaaaag tcatttacgg
     3601 aaaatatata tagacggggt gagtaatatg caagaacatt tggtggttac acttgatgga
     3661 aaagattatc ttgtagaacc aggtacgaat ttacttgaat ttattaaatc acaagatact
     3721 tttgtacctt caatttgtta taacgagtcg atgggaccaa ttcaaacatg tgatacatgt
     3781 actgttgaga ttgacggtaa aattgaacgt tcatgtagta cggtgattga tcgtccaatg
     3841 actgtaaata ctgtgaacaa tgatgtgaaa gatgctcaaa aagaagcgct tgatcgaatt
     3901 ttagaaaagc atatgctgta ttgtacagta tgtgattata ataatggtga ttgtgaaatt
     3961 cataatacga tggatgcatg ggggcttcag catcaaacgt atgaatataa agagaagcca
     4021 tacgaaaaag attacggccc attttatcgt tatgatccaa atcaatgtat cttatgtggg
     4081 cgttgtgtag aagcatgtca agatatcgaa gtgaatgaaa ctattagaat tgattgggat
     4141 cgtgaacatc cacgtgttat ttgggataat gatgtaccga ttaatgagtc ttcatgtgta
     4201 tcttgtggtc aatgtgcgac ggtatgtcca tgtaatgcca tgatggaagt gaatatggaa
     4261 ggtaatgcgg gttatatgac tgatacggaa cctggttcat tagcagcaat gattgattta
     4321 actaaaaaag cagaacctgg ttatggtcca ctatttgcaa tctcagattc agaagcagaa
     4381 atgcgtaaag agcgtattaa gaaaactaaa acagtgtgta cttattgtgg tgtgggttgt
     4441 tcatttgaag tttggactaa agatagagaa attttaaaag tacaaccatc acatgattct
     4501 ccagcaaata aaattgcgac ttgtgttaaa ggtaagtttt catggggaca tattaattca
     4561 gatcaacgat taactaaacc actagtaagg aaaaatggtg agttccatga agtagaatgg
     4621 gatgaagctt taaacgtcat tgcagataat tttacagcta ttaaagaaaa gcatggccca
     4681 gatgcactat cattcatttc ttcttctaaa gcgacgaatg aagaatcgta tttaatgcaa
     4741 aaattagcaa gacaagttat tggcacaaat aacgttgata actgttcaag atattgccaa
     4801 gcacctgcaa caaaaggctt atttagaacg gttggacacg gcggtgactc aggtagtatt
     4861 gaagatttag aaaaagcggc aatgtctgta ttgataggta ctaatacagc cgaagctcat
     4921 ccagttatcg catcacgcat gaaacgtgca caaaaattat ttggtcaaaa aatacatgta
     4981 tttgatatta gaaaacatga aatggcagaa cgtgcggatc gtttttatca acctaaacca
     5041 ggtacggatt tagcgtggtt aagtgcagtg actaagtata ttattgatca tgatttacac
     5101 gataaagcat ttattgatga gtgggtagat gattttgatg aatattacaa atcattagaa
     5161 acatttacaa tggcttttgc tgaagaagca acaggtattc ctgaatcaga attgattaaa
     5221 tttgctgaag aatgtgctaa agctgaatct gttgtaattt gttgggcaat gggtattaca
     5281 caacaagaca ttggtagtga ctcaagtaca gcgatttcaa acttattatt agtaacaggt
     5341 aattatcgtc gtcctggtac tggtgcgtat ccattacgtg gacataataa tgttcaagga
     5401 tgtagtgata tgggaagtat gcctgataag attactggtt atcaaagtat tgaagcggat
     5461 gatattcgcg ctaaatttga aaaagaatat ggcgttaaat tgaatccaaa agctggtaaa
     5521 gataatcatg aaatggtaga aggtatacat gacggagaag tacactcatt gtacttatat
     5581 ggtgaagata cgggtattgt ggattcaaat attaattttg tacaagctgc gtttgaaaaa
     5641 ttagatttca tggtagtcca agatgaattt ttaacattca cagcaacata cgcagatgtt
     5701 gtattgccag caagtccttc acttgaaaaa gacggtactt ttacaaatac cgaacgtcgt
     5761 attcaacgtt tataccaagc attagaacct cttggtgatt caaaacctga ctggaaaatt
     5821 ttccaagcaa ttgctaatag attaggattt gattggaatt acaagcatcc tagtgaaatt
     5881 atggatgaag tagcacgctt aacacctcta tatgctgggg taagttatga tcgtttagaa
     5941 ggattcaata gtttacaatg gccagtacaa cctgatggta ctgatgagcc tattctatac
     6001 ttagaaggat tcaattttga taatggtaaa gcaaaattat tcccattatc atttgataat
     6061 tactttaagc aagatgaaat ttatgatatt catgtaaaca atggtcgatt gttagagcac
     6121 ttccatgaag gtaatatgac ttatcaaaca ccaatgatta aatacaaagt gccacgtgca
     6181 tttgttgaaa tttctccaga gcttgctgaa gatagaggca ttcatgaagg tgcagaagtt
     6241 aagctgattt ctgaaacagg agaagcggta ttacaagttc acgtgacaga tcgtgttaaa
     6301 ggtaaagaaa tctatattcc attaaataat gatgcgatgg aaaatggaga tctaggtgca
     6361 attaacttat taactaatag tgatgttgat caatacacgg atacaccatc ttataaacgt
     6421 actagctgtc gtttggaggt aattacgaaa cgtggtaagt caccgttgaa ccctaataat
     6481 ttccgtgtca ataaaaaacg tcaaccgcag tacagtgttc aagtacagaa aaaatgggaa
     6541 cgttcagatt atgttttccc gggaaatcag gtggataaat aatggctgag agaatatctt
     6601 ctaaaattag acgcttagaa aaatcagaag aacaaattaa gttagaaagt ttaaatgaag
     6661 ttacagaggc aattgcagcg aataaagata gtatattaaa agcaatcaag cttattaaaa
     6721 cattagatga tgcaaaacta ttagatgctt taaatggtgc aattcgagga cgtcaagtca
     6781 tcataaataa atttgcagtt gaacttaata aagatattta tacagggcta ttatctaata
     6841 tggcttcaat ggtattttta ttaggtgaac tcaatgtatc agatttaagc gacttcctaa
     6901 ataaagttaa taaaggatta cacgttgcca atcaagcgag tccaaatgct aagacaacta
     6961 taagaagtct attgggcgta ttgaaagatg acgacatgaa ccgtagttta acatacatgc
     7021 ttaatatgct aaaaggtatg tctcgagaag aataacatta caatgaaaaa gtgtttctac
     7081 atgcaagcag tgtaggagca ctttttttaa tttttgtaaa aattaaaaag ggtgtttttg
     7141 atggaataaa tgaataatat tgaaaaacaa aaaaatcacg tgctattgtg aatcaatcat
     7201 cgacaggcct atgaaacatc atgtattatt atgattatgt aagttattta aataaaaagg
     7261 gtgagtgcat attaattcga gaatgttaaa aacgtcagct gtaatttcta atacattatt
     7321 agtcataggt ttagtctgtc tatttatgat gaaattagtg ttagctatta ctttttttgc
     7381 tgtttcatta tcaattagtc tagttgtctt taatatgatg ttcagagata gaacaacgat
     7441 gaaagtaatc gttaacgctt cttttttaat cgtaatatta gcgattgtag tggcatattt
     7501 tttattgtct aagtaagaaa aatgaggttt tgtctatatg aagaagaatt tcaagttacg
     7561 catttcaacg ctactattga tagttatttt agttgttttt gctgtattac tcatcgtgaa
     7621 tgaaactaaa ttgtttaaaa atgatgtgaa ttactctttt gatgaggctg tttcaatgca
     7681 acaagggaaa ggtattgtac agacaaaaga agaggatggt aaatttgttg aagcaaataa
     7741 taatgaaatt gctaaagcaa tgactatttc acataaagac aatgatatga agtatatgga
     7801 tatcacagaa aaagtgccaa tgtcggaatc tgaagttaac caattgctaa aaggtaaggg
     7861 gattttagaa aatcgaggga aagtttttct agaagctcaa gaaaaatatg aggttaatgt
     7921 catttatctt gttagccatg cattagtaga aacaggtaac ggcaaatcag aattagcaaa
     7981 aggcattaaa gatgggaaaa aacgctatta caactttttt ggtataggag cattcgatag
     8041 tagtgctgtt cgtagtggga aaagttatgc tgaaaaggaa caatggacat caccagataa
     8101 ggcgattatt ggtggtgcaa agttcattcg taatgaatat tttgaaaaca atcaactgaa
     8161 tttatatcaa atgcgatgga atccagaaaa tcctgcgcaa catcaatatg cgagtgacat
     8221 tcgctgggca gataaaattg ccaaattaat ggataaatcc tataagcagt ttggtataaa
     8281 gaaagatgat attagacaaa catattataa ataagacatc ggtgcttaaa ggagctggaa
     8341 caatttattg tttcgagctc ctttagcgca ttctgagtgt gttagttaaa tggattttaa
     8401 cctaacaaaa aacgctatat agcatcaaat atgctatatc ccacatcatt gttacaaatg
     8461 tacatgatgt aaatgaatat tgctgtctaa atgtgcatgt aatatacaat ggtgcagata
     8521 atacacttaa gtccttaaaa atgaaacgtt agttccaaga gtcattttta aacaatagtg
     8581 catgtgataa aatagaaaag aatgaaaaat atagaggtga caatatgaag atagcaatta
     8641 taggtgcagg catcggtgga ttaacagctg ctgcattatt acaagaacaa ggtcatacta
     8701 ttaaagtctt tgaaaaaaat gagtcagtta aagaaattgg cgctgggatt ggtatcggag
     8761 ataatgtgct taaaaaacta ggtaatcatg acttagctaa aggtattaaa aatgctgggc
     8821 aaatcttatc tacaatgaca gtgttagatg acaaagatcg cctgttaact actgttaaat
     8881 taaaaagtaa tacattgaat gtgacgttac cacgccaaac attaattgac attattaaat
     8941 cttatgtaaa agatgacgca atatttacaa atcatgaagt cacgcatata gataatgaga
     9001 cagataaagt taccatacat ttcgcggaac aagaaagtga agcatttgat ttatgtattg
     9061 gtgctgatgg aattcattct aaagtgagac aatctgtaaa tgctgacagt aaagtattat
     9121 atcaagggta tacatgcttt agaggtttaa ttgatgatat tgatttaaag catccagatt
     9181 gtgcaaaaga atactgggga agaaaaggaa gagtaggtat tgttccgtta ttaaataatc
     9241 aagcatattg gttcattaca attaactcga aggaaaacaa tcataaatat agttcgtttg
     9301 gtaaacctca tttgcaagca tactttaatc actatccaaa tgaagttaga gagatcttag
     9361 acaaacaaag tgaaacaggt atcttattgc ataatattta tgatttgaaa ccactcaaat
     9421 cttttgttta tggtcgtact attttactag gagatgcagc acatgcgaca acgcctaata
     9481 tggggcaagg tgctggacaa gcaatggaag atgctatcgt attagtaaat tgttttaacg
     9541 catacgactt tgaaaaagca ttacagcgtt atgataaaat acgtgtcaaa catactgcaa
     9601 aagtaattaa gcgttctaga aaaatcggta aaattgccca atatcgtagt cgtttatttg
     9661 ttgcagttag aaatcgtatt atgaaaatga tgccaaatgc attagcagct ggacaaacta
     9721 aattcttata taaatcgaaa gaaaaataat acaacaatat gaaaaccccc gtatgttgaa
     9781 acgagagctc aacatatggg ggttcttgtt tttataatgt tattataata aattcaatta
     9841 ttagttaacg acaaattgtg gtttctcacc ttgaacggca ctaattgcag cattagcaac
     9901 aattttagac atcatgtcac gtgcttcaaa tgtagcatta ccaatatgcg gtgttaatac
     9961 tacattatta agtgatttta agtcatcggt aatatctggt tcaaattcat atacatcaag
    10021 tgcagcacct tcaatttcat tatctttcaa tgcttgcact agtgcttgtt cgtgcacgat
    10081 tggaccacga gaggcattga ttaaatacgc cgtagatttc atcattttaa attgttctgt
    10141 atcaattaaa tgatgcattt taggattata agcagcgttg atagtgataa aatctgcatt
    10201 ctttaatagt gtatctaaat ctacatattt tgcaccgatt tctcgttctt tttcttcttt
    10261 gcgattaggt ccagtgtata gcacatccat gtcaaatgct cttgcacgac gagctactgc
    10321 actaccaatt tcacctaaac cgataatgcc gattgttttc ccagatactt ctctacctct
    10381 gaaaaataaa ggtgcccatc catcaaatcc agttgtacgt gataattggt ccccttcaac
    10441 aatacgacgc gctactgcaa gtactaatcc aattgttaaa tcagcagtcg cgtttgttga
    10501 tgctttaggt gtgtttgtaa catctatact tttttctcgg gcatactcga tatcaatatt
    10561 attaaaacca gcgccatagt tggcaatgat ttttaagtct ttaccagcat cgataacatc
    10621 tttatcaacg tttgtagata ataaactaat taaggcagtc gcgtttttaa cacctttaat
    10681 taaagtgtct ttatcgacta atcctttacc ttcatacatt tcaacttcaa aatgttcttg
    10741 taaaagtttt aaacctactt ctggtattgc accagcaaca taaacttttt ccataaaaga
    10801 tcactccttt tatcttagta tagtagaaga ttagacagta tacaactatg tcatgatgtc
    10861 ttgtgtatca atgatgtaag cgcgtacttt tgatggaggt gatataactt aggcactgta
    10921 gaactatgaa tattgtaatg tggaaaaact ggatcaatta aattagataa cgtagtttta
    10981 aagttaatag tattagaaaa aattaatatt ttgaatatgg gaggaaatat aaataagtag
    11041 gtggcaacga aaaatagcaa aaaaagagct tctcctataa aggaaagctc aaagtttttt
    11101 gatgacatat gtactagaat taagtttcaa gacaatatgt atcatcgtgt ttatattaaa
    11161 tatggatgta gttgtagtta cctgcttcac ttgcagaaat agttctagaa cttactgaga
    11221 aaggtccgcc actataattc atttctgaaa ttgtaactga accatcactg tttacacttt
    11281 ctacatatgc aacgtgacca aatggtcctt cagaagattg taggatagcg cctttagaag
    11341 gtgtatgatt tactgtgaat ccagcacctt gtgcagcagc agcccaatta ttagcatttc
    11401 cccaagtaga accgatttct ccgccaactt tatcatatac ataccaagta cattgtcctg
    11461 cagtgtataa gttaccagaa tgtgaaattg atgatgtagt tgtcgtagtt gtcgtagtcg
    11521 ttgtagtttg agtcgtgttg tagttatagt tgttgtaatt tgtataattt tcagcagcat
    11581 ctgcatgatg tgcttgacct actaatgctg tgccgattcc tgctgttaac gtagttgctg
    11641 ttactaattt tttcatgaat aaagtcctcc aaagttctat atcttttttt ataaataaaa
    11701 cgtagcgact gttttattct cacatctcga attgatgaca atagttactt taacaaaatt
    11761 aatgcttctt gtggggaatg ttattgattt gtaaaagaat aaaaaaactt tgactaattt
    11821 tgtaataaaa attagtcaaa gttacaatga gattaacaga taattaatag gaaatattta
    11881 tttgtaatat gtttaaataa atcgaattgt taaaggtatt atatattctt ggccattata
    11941 atatttgaca cacgcaataa ttgtgaatac aaaagataat attgagaaag cgaatatgga
    12001 taaaataccg ataaacgtaa tgatgaaacc tataataata atgaaatcaa tatctgtagc
    12061 aattaggaaa acgcctatta aagtgataac gactaaaacg atagaccaaa taatataaga
    12121 aatcgtatag ttaagataat tttttccagc acgatcaact agtttcgatt catctttttt
    12181 caataaccat attatcagtg gaccaataat agatgtgaat aaacttaata aatagataag
    12241 catcgccata atgttctcat cattggattt gcgattcggt tgatgatttg ttacgtcgtt
    12301 catttcagtt gtcatattag acactccttt gaaaattgta atattatctt taactataac
    12361 aaaatataat caaaaataaa catgtttatt aaacaattat taaaaataaa aataattggt
    12421 ggacgtcggc gtttaaatag gttaatttaa ggttatatat acttaacatt tataatgatg
    12481 cgtaatgaat tcgcatcatt tttatattgt cttacgtata atttgttttt aattttaacc
    12541 aaagatagaa agagggttgt ttatgaaaat agcaattgta ggatcaggaa atggcgcagt
    12601 tacggcagca gtagatatgg tgagcaaagg ccacgatgtt aaattatatt gtcgtaatca
    12661 atctataagt aagtttcaaa acgcaatcga aaagggcgga tttgatttta ataatgaagg
    12721 tgatgaacgt ttcgtaaaat tcactgatat tagtgatgat atggaatatg ttttaaaaga
    12781 tgctgaaatt gttcaagtga ttattccatc ttcatacata gagtattatg ctgatgtaat
    12841 ggcagagcat gtaactgata atcagttgat attcttcaac atggctgcag caatggggtc
    12901 aattcgtttt atgaatgttt tagaagatag acatattgaa acaaaaccac aactagcgga
    12961 agctaatacg ttgacgtatg gtacgcgtgt cgattttgaa aatgcagcag ttgatttatc
    13021 tctaaatgta cgtcgtatct tcttttcaac atatgataga agctgtctaa atgattgtta
    13081 tgacaaagtt tcaagtattt atgatcattt agtaaaagag gaaagcttaa ttaaaacaaa
    13141 tcttgaaaat ggtaatcctg aagtgcatcc aggaccaaca ttattgaatg tcggtcgcat
    13201 tgattatgct ggcgagttcg ctttatataa agaaggaatt actaaacata cagttagatt
    13261 acttcatgca atcgaattag aacgtttgaa tttaggccgt agattaggtt ttgaattatc
    13321 aacagctaaa gaatcacgta ttgaacgtgg ttatttagaa cgtgataaag aagatgaacc
    13381 attaaatcgt ttgtttaata caagcccagt attttcacaa attccaggac caaatcatgt
    13441 agaaagcaga tatttaactg aagatattgc atatggttta gtactatggt caagcttagg
    13501 tcgtgttatt gatgtaccga caccaaatat agatgcagta attgtaattg catcaaccat
    13561 tttagagaga gacttctttg aggaaggctt aacagttgaa gaaattggtt tagataagct
    13621 tgatttagaa aaatatttaa aataaatgat ggcttgaaga tagaaaagga tatagcatta
    13681 tgcaaaagca ataaattgaa gaaaagaggt ttctcatcaa taagcgaagg ggacgataga
    13741 tgatgaaaag aaaacccacc tttttagaat caatttcgac aatgattgta atggttattg
    13801 ttgttgtaac aggctttgtg ttttttgata ttccaattca agtattatta attattgcct
    13861 cagcatatgc cacatggatt gcaaaacgtg taggcttaac atggcaagat ttagaaaaag
    13921 gcattgcaga acgtttaaat actgcaatgc ctgcaatttt aattatacta gcggtaggaa
    13981 ttatagtagg cagttggatg ttttctggca cagtgccagc cttgatttat tatggcttag
    14041 atttattgaa tccaagctat tttttaatat cagccttttt tataagtgct gttacatctg
    14101 tagcaactgg tacagcatgg ggctctgcat caactgcagg gattgcactt atttctattg
    14161 gtaatcaatt ggggattcct ccagggatgg cagcgggtgc tattatagca ggggctgtgt
    14221 ttggcgataa aatgtcacca ttatcagata caactaattt agcggcgctt gttactaaag
    14281 ttaatatatt taaacatata cattcgatga tgtggacgac gatacctgca tcaatcatag
    14341 gtttattagt atggtttatt gctggatttc aatttaaagg gcattcaaat gataaacaga
    14401 ttcaaacttt gttatcagag cttgcacaga tttatcaaat taacatatgg gtctgggttc
    14461 ccttaattgt gatcattgtt tgtttgctat ttaaaatggc tacagtgcca gctatgctaa
    14521 tatcaagctt ttctgccatt atagtgggga cttttaatca tcatttcaaa atgacagatg
    14581 gtttcaaagc aacatttagt ggttttaacg aatcaatgat acatcagtct catatttcat
    14641 ccagtgtgaa aagcttgtta gaacagggtg gtatgatgag tatgacccaa atattagtaa
    14701 cgatattttg cggatatgca tttgcaggta ttgtagaaaa agcaggatgt ttagaagtct
    14761 tattaactac tatttctaaa ggcatccatt ctgtaggaag tttaatatgt attactgtta
    14821 tttgttgtat tgcgcttgta ttcgctgcag gtgttgcttc gattgtaatt attatggtcg
    14881 gtgtgttaat gaaagatttg ttcgaaaaat accaagtctc tcgttccgtt ttatcaagaa
    14941 cacttgaaga ctcaagtacg atggtcttac ctttaatacc atggggaaca tcaggtattt
    15001 actatacgaa tcaacttcat gtctctgttg aagaattttt catatggaca gtaccatgtt
    15061 atttatgcgc aattatagca attatctatg gttttacagg gataggtatt aaaaagtcat
    15121 cgaattcacg tttaacttaa tgtgagcgtg gaatatatat aatatgttga aacactttaa
    15181 tcatttataa ttgtagcggt tataatttga aaaggtttta acttagaata aatatcctct
    15241 atacatatac tgaatatgtt ttgtagcgga acatgttgat atatgtaatg taagttttat
    15301 gtcatgattt gtaatgacta aattaattga gaatttgaag gcaagtatat ttgtaagtac
    15361 tttaactaaa aatttatcaa tgtatagccg atttgacatg cctaaatttg ggtgtgtcaa
    15421 tggctgtatg ttgtttattc tttattacag agtgaatcgg attggtgaaa atcgaaattt
    15481 tgagattttt accaattcga tttttttcat agaaattaaa aaagccaaca aggctcttga
    15541 aaccttgttg gcgtaaacat agccatcact aattagtgaa tgaagttata accagcagct
    15601 tggctagctg agattgtacg tgaagttaca acacctgggc cataaccata gttcatttct
    15661 gaaactctta ctgaaccatt gctgttaaca ctttcaacgt atgcaacgtg accgtatgca
    15721 ccttgagttg tttgcataat tgcaccagct tttggtgtat tgttcactgt gtaaccagct
    15781 cttgcagctg cgttagccca gttacttgca ttgccccaag ttgaaccgat tttaccacct
    15841 acacgatcaa atacgtagta tgtacattga ccagaagtgt ataagttacg tcctgaagta
    15901 taaccacttg agattgaacg gccatttgat gatggagcca tagttgtagt tacttgaaca
    15961 ttgttgcttg aagtgctgta gcttgcacct aaaccaccag tacggtagct gtttgtgttg
    16021 taactattat agttattgta gttatatgat tgattattat ttgagtagtt gttgtaacgg
    16081 ctgtagttat tgtagctata accgttgttg taattgttat agttattgta accattgtag
    16141 tagtaatagc tgtagtagcc attatcttgg tttaattgac ttggatgcca gttacctttc
    16201 catgtgtaat ggtagttacc ttgtgcatca atagtgtaag tatagctata tgatgttggg
    16261 tcgtttggat tataaccgta gttatcttgc tcagaagcat gagcttgatt tcctgatgca
    16321 attgcgattg tagcgaatcc tgcagttgcg atagtagctg tagcgatttt cttcatttta
    16381 aaaatatcct cctaaaaatt ttaaatctaa aatattttcg taatgtccgt gtgacaaaat
    16441 taatgttata agttatctct cgtaattaaa cgacaagaaa gactataaca gaaattagcg
    16501 tccttgtgtg ctttgttaac gttttgtaat tttttgctaa tatcttgaca caatagaatt
    16561 ttaaaagtat agaaatttgc attttgcaaa acttataact acggcattct ttgtgaaaac
    16621 tgaatgtttc gaaaataagt ctgttacaaa tttgtaatat tactgaaaat tctaaatgta
    16681 tattttgtgc ataatatagg acttttaatc agaaaatgca taaaaattcg acacgaacga
    16741 tcaagtttct atgattatga aagctttggt atttggatga aaatgtatat ttaatggata
    16801 aaatatccta atttagcata aaaaaatgtt ttaataaaag tattatttga tataatcgat
    16861 ttatgttttg ttactgctaa aaaacatgtg gcgatataat tggtagtgaa tattagatgt
    16921 tgacaattga tttgagaaag acaaacagga gtaaatcaat atatttgtat atgtgaggat
    16981 gtaagatgac agataattat tcactacata tttataaaag tatgaaaatg agtagtgtga
    17041 aggaagcgaa cttaaaaatt atatattggt tacaaggtac aggtgtagtt tcaatcaatc
    17101 tacaacaata tgatctaaaa agaaattatg tcaccattat tatgttgaat gatttgtatc
    17161 aaattgatgg taatgatgat agtgtatgtt gtgttgttga agtaagtgca aagacatttt
    17221 tgaagtttat gaatgcaaac tatatgatgc gtggcaagat attaacaaat gaggttgcta
    17281 gcacttttag aatcttgatt aagtatttaa tctacagcaa aattaaacaa cagtcttata
    17341 atgctaatga taagattatt aattacatgt gtgttgaatt aatgtcatta aatacatacg
    17401 atggtagtcg tcatttagta gccgaagaag tgcacgatta tttaacaaac aaccaccata
    17461 aaaagataaa taggaaagat gtcattaatc aagttagtat tacaaacaaa gctttaagtg
    17521 acatgtttaa agcgacacct tatagtaatt ttattcaata tttaaatcat attagactgg
    17581 agcattgctt aattgatatt ttaacttcta agaagcctat tgaagaaatc gcaagtagtc
    17641 atggttttaa tcattattct cgttttatac acctctttaa agaaacgtat ggtaacactc
    17701 ctaaattaat tagaaaaaga tatagtccaa catctcattc aattaaccat tcccaattag
    17761 ttgaaattga taaaaatata attgaattat ttgatgatac taatcaaagt tcttcaagta
    17821 tgcgcgagat tgatatacct tttgagccta caaaaaggag tcaaatgtat caacctaaaa
    17881 acatttatat taaaggaagt aatttttatt ggatggatta ttatactatt aaacaaatca
    17941 ttcaaagatt aggtgttaat cctgaaaact tgcatataat tgttacaatc gacacaagca
    18001 atgagctatc gattgaacaa atcactttat tacttcaaaa gatagtgaat tacaatgtaa
    18061 atgtagtgtt tcgtattaaa catgaatata aggaattgtt aacgagtgct gaaagaggca
    18121 agatagaaaa gctagtagct acaattttta atatgacttt tgagagtaat agatgtaaga
    18181 ttgcattttt aattaacgac ttaaatacta ctgcaattca aaaactgaaa cgtttaatag
    18241 ataattatat aggagtattt gaattaatat atcatctgga accaaaagag ttgaatacgc
    18301 gctatgataa agaaattgat catctaatag actattttat cttaccgcta agacaaatag
    18361 aacaggtgtc tattgcacgt tctaaaatta tatgtgatgt agatttgatt
//
LOCUS       DAKTGS010000046        18259 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.046, whole genome shotgun sequence.
ACCESSION   DAKTGS010000046 DAKTGS010000000
VERSION     DAKTGS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18259
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.046"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..174
                     /locus_tag="O0286_001302"
     CDS             <1..174
                     /locus_tag="O0286_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0169686.1"
                     /translation="KRYDIPTNKAPKLLLKGTGNLKGSSVGYKKIEFTFLENKNENIY
                     FTDSLHLEPSEDK"
     gene            238..1008
                     /locus_tag="O0286_001303"
     CDS             238..1008
                     /locus_tag="O0286_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0169687.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            1075..1842
                     /locus_tag="O0286_001304"
     CDS             1075..1842
                     /locus_tag="O0286_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0169688.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            1936..4173
                     /locus_tag="O0286_001305"
     CDS             1936..4173
                     /locus_tag="O0286_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0169689.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            4324..5502
                     /locus_tag="O0286_001306"
     CDS             4324..5502
                     /locus_tag="O0286_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0169690.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            5504..6892
                     /locus_tag="O0286_001307"
     CDS             5504..6892
                     /locus_tag="O0286_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0169691.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(7385..9046)
                     /locus_tag="O0286_001308"
     CDS             complement(7385..9046)
                     /locus_tag="O0286_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCY0169692.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(9372..11147)
                     /locus_tag="O0286_001309"
     CDS             complement(9372..11147)
                     /locus_tag="O0286_001309"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCY0169693.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(11321..12193)
                     /locus_tag="O0286_001310"
     CDS             complement(11321..12193)
                     /locus_tag="O0286_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0169694.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            12306..13703
                     /locus_tag="O0286_001311"
     CDS             12306..13703
                     /locus_tag="O0286_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0169695.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKV"
     gene            13893..14366
                     /locus_tag="O0286_001312"
     CDS             13893..14366
                     /locus_tag="O0286_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCY0169696.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            14628..16220
                     /locus_tag="O0286_001313"
     CDS             14628..16220
                     /locus_tag="O0286_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0169697.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(16549..18075)
                     /gene="spa"
                     /locus_tag="O0286_001314"
     CDS             complement(16549..18075)
                     /gene="spa"
                     /locus_tag="O0286_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCY0169698.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
BASE COUNT         6098 a         2753 c         3314 g         6094 t
ORIGIN      
        1 aaaagatatg acataccaac aaataaagcg ccgaagctgt tgttgaaagg tacagggaat
       61 ttaaaaggtt catcagttgg atataaaaaa attgaattta cttttttaga gaataaaaat
      121 gaaaatattt actttactga tagtctacat cttgaaccga gcgaggataa ataatcatct
      181 tttaaccaga atatgaaatc aatctaggac aacctaataa aaggagagag ttgtaagatg
      241 atgaaacgat taaacaaatt agtgttaggc attatttttc tgtttttagt cattagtatc
      301 actgctggtt gtggcatagg taaagaagcg aaaataaaga aaagttttga gaagacattg
      361 agtatgtatc cgattaaaaa tctagaggat ttatacgata aagaaggtta tcgtgatgac
      421 gaatttgata aaaatgataa aggtacatgg attattggtt ctgaaatggc aactcaaaat
      481 aagggggaag ctctgaaagt taaaggtatg gtcttgtata tgaatagaaa taccaaaaca
      541 acaaaaggat attattatgt aaatgcaata aagaatgata aagacggaag accccaagag
      601 aatgaaaaaa ggtacccagt taaaatggtc gataataaaa tcattccaac aaaagaaatt
      661 aaagataaaa acataaaaaa agaaatcgaa aactttaagt tctttgttca atatggaaac
      721 tttaaagatt tgtcgaagta taaagacgga gatatttcat ataatccaga ggtgccgagt
      781 tattcggcta aatatcaatt aactaatgat gattacaatg taaagcaatt acgtaaaaga
      841 tatgacatac caacaaataa agcaccgaag ttgttgttga agggtacagg gaatttaaaa
      901 ggttcatcag ttggatataa agacattgaa tttacgtttg tagagaaaaa agaagagaac
      961 atttacttta gtgatgggtt aatttttaaa ccaagtgagg ataaataatc atgactcgat
     1021 caaaatatga aatcaatcca ggaaagataa taagaagaat aggattgtga aattatgaaa
     1081 cgattaaaca aattggtatt gtatattagt tttttgatat tagtcattag tttcactgct
     1141 ggttgtggca taggtaaaga agcggaagtt aagaaaagct ttgaaaaaac attgagtatg
     1201 tatccgatta aaaatctaga ggatttatac gataaagaag gttatcgtga tgacgaattt
     1261 gataaaaatg ataaaggtac atggattatt ggttctgaaa tggttgttca acctaaaggg
     1321 gaacgtatga aatcaaaagg tatggttcta tatatgaata gaaacactaa gactacaacc
     1381 gggaagtata tagtgagtga aacacttcat gatgaagatg gtagacctaa gagtaaagat
     1441 aaagaatatc ccgttaaaat ggtagacaat aaaatcattc caactaaagg tatcaaagac
     1501 gaaaatataa aaaaagaaat cgagaatttt aagttcttcg cgcaatatgg cagctttaaa
     1561 gatttgtcga agtacaaaga cggcgatata tcatataatc cagaggtacc gagttattca
     1621 gcaaaatatc aattaactaa tgatgactat aatgtaaaac aattacgaaa aagatataaa
     1681 ataccaacga ataaagcacc aaagttattg ttgaaaggtt cgggagactt aaaaggttca
     1741 tcagttggat ataaagacat tgaatttact tttgtggaga aaaaaggtga aaatactttt
     1801 tttactgata gtctacatct tgaaccgagt gaggataaat aatcatccac acacacgatt
     1861 caatatgaaa ttcaaatatg ttgctgtaaa ataatgtaaa ataaacgtat tcatattact
     1921 gaattgaggg atagtatgca acgtgattat ttaattcgag tagaaactga gagtatgtca
     1981 gatttcaaaa ggctcaatag tttaatgatt ggttttgtta ttaaaggtga ggcacacatt
     2041 tatgatgaaa ataatatgac gcaatgcaac agtggcgaca ttttcatcat taaccaccgc
     2101 gacttgtatc gatttcaact tcaacaagat ggcatcatat gttatatcca attccaaatg
     2161 aaatatttag cagacaagtt tgatgatgtg cattgtctat attttcactt aacagatgcg
     2221 accacaacaa agaatataca tcaactgaga aatataatgg caagactggt ttcaacacat
     2281 atccgacata atgagttgtc taaattgact gagcaacaac ttgtgattca gttgcttatg
     2341 catatgattc attatgtccc gcgtacatat cattcgaacc aaagtatctt aaatgatgat
     2401 aaagtgaatc aagtatgcga ctatatcgag ttacattttc atgaagattt aagcctttca
     2461 gaattaagcg aatacgttgg gtggtcagag agccatctgt ctaaaaagtt tacagaatcg
     2521 ctaggtgtag gattccatca tttcttaaat acgacgcgaa ttgagcatgc gaaactcgat
     2581 ttgacataca cagatgaaac cattactgat attgcattgc aaaatggctt ttcaagtgca
     2641 gcgagctttg cgagaacatt taaacacttt acgcatcaaa cacctaaaca atatcgaggt
     2701 gatcgtccag caatcactga aaaccagcaa tcggcacaac atgattatca cgaccgtgaa
     2761 ttgatattac ttttaaatga ctacattgaa gaaatgaatc attttattga agatattgaa
     2821 aagatgaact ataaagagat tgcctttcaa ccaaccaatc accaactaaa tcaatttaat
     2881 catattattc aagtgggcta tttgaggaat ttgctcaata cacagtatca atcacagttg
     2941 cttacatgtc atcatgattt tcaagtcaat gaagtattag catatgatgt gattccatat
     3001 atcatgaaaa agctcaatgc gccattcacg tatgatgcag aaatttcgaa tatattttat
     3061 gatattgatt tgtgtttaga ctttttatta gatcataact ttagtttgac catgcatttg
     3121 gatcagtatg actcacgtga ttatatcgat gcatttaaaa tttttatcca tcacgttgcc
     3181 ctgcatgtca gtcatagaaa agatttgaag ttcaacttgt atgtgacgac attgcacacg
     3241 tctttgattg aaatgattga ttattttaaa gcattattcc ctaatggtgg cttgtacatt
     3301 cacttagatc aagctacgga aagacatcta ccattattga aacgacttga gccacacatc
     3361 gaccattttg tatttgatgc caattcaaat gacgctgttg attttaataa aatgaatgat
     3421 gatgaattta aaaccgcgag tcaaatgatt attaataaaa cgaattacct tatcgactta
     3481 atgcatcgtc atcgcctaaa gcgtccactc attttactca attggaatac attgacgggt
     3541 gatacattta ttacaaacgg cgaatatttt agaggtggta tcatcattga gcaattatta
     3601 aaattaagtt ctaaagtaga gggtatcggt tattggttga attatgattt gcacgtcagt
     3661 cattgtaaaa atgaacggga ttatatgaat tctattgaac tatttcatca atataatgga
     3721 aaacgtccgg tctatttcac ggcattgcta tttaataaat taacaagcaa tattttgtat
     3781 tctgatgata catgtattgt cacggggact gattcaaatt ttcaaatatt gttatatgat
     3841 gcaaagcatt ttaatccgta cttagcgttg gacaatcaaa tgaatatgcg tgcgacggaa
     3901 atgattcatt tgaacattaa tgccctggaa gaaggtatgt ataagattaa acattttacc
     3961 ttagataaag aaaatggtgc gttatttaat ctttggcgca aacatcatac gatacatggc
     4021 atggacaagg actctataga ttacgttaat cgaatgagtt ttccgaaatt agaagtatat
     4081 gatatagata tcacggacac actggcatta aacattaaaa tgattacgaa tgggattcac
     4141 ttaattgaag taaaacgtta cccaagttca taaaatgatc acaaatcaca aattttgata
     4201 tacataattt gtgatttttt atattcaaag ctaaaattgc aaaaaattaa tggttaacat
     4261 ctctgttgtt ggcaatataa ataatgatta atcatttatg atgtaactaa ggagatgaag
     4321 gatatgaatc aacaattaat tgaaacttta aaatctaaag aaggcaaaat gattgagatc
     4381 agacgttatt tacatcagca tccagaatta tcttttcatg aagatgaaac ggcgaaatac
     4441 atcgctgaat tttacaaagg taaagatgtg gaagtagaaa cgaatgtcgg accacgtgga
     4501 attaaagtaa cgattgattc agggaaacct ggtaaaacat tagcaatccg tgcagacttt
     4561 gacgcattac ccattactga agatacagga ttatcttttg catcacaaaa taaaggtgtt
     4621 atgcacgcat gtggtcacga tgcacataca gcatacatgc ttgtattagc agagacgctt
     4681 gctgaaatga aagatagttt tacaggaaaa gtcgttgtga tacatcaacc agctgaagaa
     4741 gtaccaccag gtggtgctaa aacaatgatt gaaaatggtg tattagacgg tgttgatcat
     4801 gtattaggtg tacacgtcat gagcacaatg aaaacaggta aagtgtatta cagacctggt
     4861 tatgttcaaa caggacgcgc attcttcaaa ttgaaagttc aaggtaaagg tggtcatggt
     4921 tcatcaccac atatggccaa tgatgccatt gttgcaggta gctacttcgt cacagcgtta
     4981 caaacagttg tatctagacg actaagtcca tttgaaaccg gtgttgtcac aatcggttca
     5041 tttgacggta aaggtcaatt caatgtcatt aaagatgttg ttgaaattga aggtgatgta
     5101 cgtggattaa cagatgctac aaaagcaaca attgaaaaag aaattaaacg tttatcaaaa
     5161 ggattagagg atatgtatgg tgtaacttgc accttagaat ataacgatga ttatccagca
     5221 ttatataatg atccagagtt tactgagtac gtggctaaga cgttgaaaga agcaaacctt
     5281 gattttggtg ttgaaatgtg tgaaccacaa ccaccttcag aagactttgc atattatgct
     5341 aaagaacgcc caagtgcctt tatttataca ggtgcagcgg tggaaaatgg tgaaatttac
     5401 ccacatcatc atcctaaatt taacatttca gaaaaatcat tacttatttc tgcagaagca
     5461 gtagggacag ttgttttaga ttaccttaaa ggagataact aacatgaatg aaacgtatcg
     5521 cgggggcaac aagttaatct taggtattgt attaggtgtt attacatttt ggttgtttgc
     5581 acaatcactt gtaaatgttg taccgaattt acaacaaagt tttggtacag acatggggac
     5641 aattagtatt gcggtcagtc taactgcact attttcaggc atgtttgttg ttggagcagg
     5701 tggtctggca gataaaattg ggcgcgtgaa aatgacgaat atcggtttat tattaagtat
     5761 tattggttca gcattaatta ttattacgaa tttaccggca ttattaattt taggtcgtgt
     5821 tatacaaggt gtatcagcag cgtgtattat gccttctaca ttggccatta tgaaaactta
     5881 ttatcagggt gctgaacgtc agcgtgcctt aagttattgg tctatcggtt cttggggtgg
     5941 cagtggtatc tgttcactct tcggtggtgc agttgcgaca actatgggtt ggagatggat
     6001 tttcatcttc tcaattatcg ttgccgtact ttcaatgtta ctcatcaaag ggacgcctga
     6061 aacgaaatca gaaattacca atacacataa atttgacgtt gcagggctaa ttgttctagt
     6121 agttatgttg ctaagtttaa acgttgtcat tactaaaggt gcagcacttg gttacacatc
     6181 attatggttc tttggtttga ttgcaatcgt aattgtagca ttctttattt tcttaaatgt
     6241 tgagaaaaaa gtagataatc cacttattga ttttaaatta tttgaaaata aaccatatac
     6301 aggtgcaacg atttcgaact tcttattaaa cggtgttgca ggtacattaa ttgtagcgaa
     6361 tacattcgtg caacaaggtt taggttatac agcattgcag gcaggatact tatcaattac
     6421 ttatttaatc atggtgttat tgatgattcg agttggtgaa aaattattac aaaaaatggg
     6481 ttctaagcga ccaatgttat taggtacatt cattgtggtc attggtattg cacttatttc
     6541 attagtattc ttaccaggca tattttatgt tatcagttgt gtcgtaggat atttatgttt
     6601 cggactaggc ttaggtattt atgcaacacc ttctacagat acagctattt cgaatgcacc
     6661 gttagataaa gttggcgttg cttcaggtat ttataaaatg gcttcatcac ttggtggcgc
     6721 attcggtgtc gcaattagtg gtgctgtata tgctggtgca gttgctgcaa cgagcattca
     6781 tacaggtgcg atgattgcac tttgggttaa cgtattaatg ggaatcatgg catttatcgc
     6841 aattttattc gcgattccta atgatgataa acgtgtcaaa gatgcgaaat aataacgatg
     6901 acacgccaag tgttttgata atgatataaa tgaaagtaaa gcaaattaaa tgaggaaatg
     6961 tatagagcag tgcatataga taaatctatg attggattaa cactaatata aatataaatg
     7021 aaaatgaaaa accatccact taaatttttg ataagttagg tggatggtta tttattgcgt
     7081 tgttctaaaa atttggacat ttatatggaa agaactatat ataatgtgaa ggttatggca
     7141 attcgcttca atgaaggcga cgtgtaaaag tgtatattaa acgtttcggg gtcagactcc
     7201 gagaacagtg tccgtcatgc ttaccgcata ctaatgaagc attattatat cagtgtttat
     7261 agccgattta agataataaa ttttgctaaa caaaaaagcc aacccatgaa tgttggattg
     7321 gctttttaca tgcatctgaa tctctaattt taaaaaaata tgaatataaa taagacagta
     7381 aaaattaaat ttcagttgtt gcaatttctt catctgtagg tacatcatcg ttaaggccaa
     7441 caagtgcttc agaaacattt cgtgaatgat aaccgatacg ttcaagaaca ccaatcatat
     7501 cgatatatag taatccgcct tttgttgtac attcaccacg attaaggcgt ttaatatgac
     7561 ctttgcgtag tttatgttca atattaaatg attctctact acgttctaca atttcatctt
     7621 ttttcgtttt gtcataaaca tctaacatgt cgatggcttt atcaaatgac tcagcaacat
     7681 ggttgaataa tttatccata ccgcgttgtg catcttctgt aatgcgaata tcttcatcat
     7741 gttggcgttt taattgagcg acatactctt ctgttagctc tgctactttt aaaatagagc
     7801 gattgacatc aaacataact gctaaacgct caacgtctgc cttcgtaatg gcttttgtag
     7861 aaattctaac taaataattt cgaatgctat cattgattgt ttcaacagct tgatgctttt
     7921 gttcaagctt tttgatcaat tttttatcgt cttttgtaat ttcgcgaatg tcttcaaaca
     7981 ttgataagac aatctgaccc acattttgta attctttttg agtttcttgt aatgcaacac
     8041 caggtgcgtg ataaacaaga tctttgttta agtgctgagg tttatagtca tcagcaatat
     8101 ctttacctgg gacaagcttt gtaacaatcc atgctaaacc tgctacaaat ggtaattgaa
     8161 tcaaagtatt tgttatgttg aagataccat gtgatactgc aatcgtcatc gctggtttta
     8221 agtgccataa atcttgtaac aaactaatca aatgaatcac aactggcaag aaaattgtga
     8281 agataattac cccgattaag ttaaagatga cgtgtacaag cgccgcacgt tttgcagcga
     8341 ttgagccggc taaactagct aagatagctg taatcgtggt accaatgtta tcgcctagta
     8401 acacagggat tgctgcgttt aagctaatta aatcttgttg ataaaattct tgtaaaatac
     8461 caatcgtcgc acttgaactt tgaactagtg ctgttaaccc tgcgccgaca atgacagcga
     8521 gtattggatt tgtagacata tcaagcatta attgcttaaa tccatctaat gatgctaaag
     8581 gtttaacggc atcacccata aattctagac cgaagaatag tgaaccgaaa ccgaatagta
     8641 tgcggccaat gttattgatt ttagagcgtt taaagaaaaa gattaagaat gcacctaatg
     8701 ctaaaattgg cattgcatat tcgcctaaat ctataccgat aataaatgca gttaccgttg
     8761 ttccgatatt agcacccatt atcactccaa tggcttgttt caatgtcata aatccagctg
     8821 ttaccagtcc gattgtgata actgtcgtac ctgaactact ttgtattaaa atagttacaa
     8881 cgatacctgc aataacacct aatactggat ttgatgtaaa tttgtttaaa atatctcgta
     8941 gcctgtctcc tgctgatgct tgaagcccgt ctcccatgat ttttaagccg taaaggaaaa
     9001 tacctaaacc acctaaaaag gagaaaatga cttctgtaac cgacatttcc attatttcac
     9061 ctcaaataag ctttatattt agattatcgc ttataattgt aaatttaatg ttaagattag
     9121 gtaaaattat ttaacaatat atgttatttg tatatgactt gtaaaatatc gtcacttatt
     9181 atgtaaattt tcagtgtgaa atggcaggtt tgcaatcact tgtttaacaa aatgatgcaa
     9241 tcaatcatgt aattatgttt catcaaaaaa atcatgagag tggaacaacg aaataatttt
     9301 tatgaaaaca tcatttctgt cccagtctca tgatttgaaa tcaccaaata aaaatctatt
     9361 aatggttttc gttataacaa tttgtgttct tttaataatg actcaatgta cgtacctttt
     9421 atctttttaa ggaatcctgc taatgcgagt ttttgcattt tcgaatcttt agtaatctca
     9481 cgcaaatctt ggtggtcatt cagttcgtat atggcatcca ttaagacgcg aagatcaaat
     9541 ggactattga tgacttctgg aataccacga tctatattta gtaattgata aacagcttcc
     9601 atggcagtac gaaccgaata ttctgttgta aatacagtgt ctcgctctgt ttctgcaaag
     9661 ttaccaataa atgctaagtt ctgagattga tgcgggacga ctaaaggtct gtcgccgata
     9721 gcacgcgtca tgaaataaga tgtgatatat ggcatataaa caggaatcgt attagatgca
     9781 tgttttgcta agtcttcaat tttgtcagtt gatacaccta agtgatacag ccattcttgg
     9841 catatttcat taccactaca ttctgtaatt ggctttttaa tataatcgcc gtttacatct
     9901 gaatataagg cataaatcca tgtagatatt tcattttcag gttggtcttt aaactgttgc
     9961 tgacgattga ttgtaaaact catttgccat gcagaatcat tgattgtaat aataccgcct
    10021 gtaactgttt tgcctgcaag tgggtcacgt ttacaaatac tttctattgt atcgataatc
    10081 tctttattgt ttgttgtaga agttgctgaa acaaaccaac tttttttagg aatattttgg
    10141 caaaacttat caggattacc aaattcagga ctttgtcgcg ctaaattttt ccatagtgtc
    10201 caactaccac ctaattcgtc agttggtggc gctggtgtat cattatcacc ataagtagag
    10261 ctttctgtaa tactaccgtt tgtcacaaag acaagatcgt ttatagtcag tttaatagat
    10321 tctgcattac cattacggtc aattaatatt tctcgggcaa ttttttgact tgtcgtaaca
    10381 tctattttaa tatcttcgac ttttacatcg tattcaaatt gaaccccatg cgattttaaa
    10441 tattcaacca taggtaatac taaagattca tattgattat atttagtgaa ttttaaagct
    10501 gaaaagtctg cgagaccact aatatgatga acgaatcgca ttagatagcg acgcatttcc
    10561 attgcagaat gccacggttc aaatgcaaac atcgttttcc agtaaatcca aaagtttgaa
    10621 ttaaagaagt catcggaaaa tacatctgtt attttgacat catctaaatc ttcttcattc
    10681 gttaagcata aatctaaaat ttctttaatc gccgttttag tcaaagtgaa gtctccgtct
    10741 gtgactaaac gttgaccctg tttctcaata acacgacagc gagaatagtt agggtcttct
    10801 ttgtttagcc aatagaactc atctaataca gacgcgttat cgatttctaa tgaagggata
    10861 gatctgaata agtcccacaa acattcaaag tggttctcca tttcacgacc accgcggaca
    10921 acatagcctt ttaaaggcat attttcacca tcaagactac cacctgcttt aggtaactct
    10981 tctaaaatat gaatcttcga accttccatt tgaccatccc ttattaaaaa acaagctgca
    11041 gcaagtgaag ctagaccaga tccgattaag taagcggatt tgttttctac attttcaggt
    11101 tttttagggc gcgcaaatgc ttcataattt ccataactgt aatacatatc caccaagtcc
    11161 tttcgtatta gaatacactc agactatacc cctttgtgaa ttgcaaatat tagtatttat
    11221 attggtaaat ttgacgaaat tagatcattt ttatattttt gatttttaaa attttatcac
    11281 tcgaatgttt ttggattctg aggtgcggga cttggaataa ttatgtattt tgaaagaagt
    11341 tacttaaact aacgcctagt agagtgataa ttccgcctat aatagcaagg gttgttggta
    11401 gctcgtctaa taacagataa gataataata aagaaacgat aggtgttaaa taaagagaca
    11461 ttgttgcatc agagacacca actgacttca caatataagc aagcaaaacg tatggaatta
    11521 tagtagggaa tatagctaaa taaagtaccg atactattga tgtaaaagtg gcgccgtgta
    11581 tatcgttgat gatttcagga ataaaaataa gcataaatgg tgagcttgcc attattgtat
    11641 atagtgtgaa agcgatgaag ccgtattttt ctatgtattt tttctggaaa gtaaaataca
    11701 aactttcact aaaagatgca agtaaaataa taaaaacacc taatacatta atagttgtgt
    11761 aatcatcttt acttattgaa ataatggata ttcctataaa tgcgacgagt gaacttaacc
    11821 aattccattt tgaaaaatgc tcttttaaaa atatataagc taaagcacta gaaaaaatag
    11881 gcgttgtaga gactagaatt ccagatatac ctgcactaat caaagcttca ccaaaattta
    11941 aagctgtgtg atatatcaca aatccacaaa atcctaaaat aaaaataaca gggatatctc
    12001 ttagttcagg ggtaggcaat ttctttataa ttacgaacgg caagagaatt attgttgcta
    12061 aaattaaacg aaatgccgac aatgattctg cactaaaatc atttaacgca atctttatca
    12121 ttggaaatgc agatccccac aatatgatag taaataaata tgatagaaaa gtagtgtctt
    12181 gaagtttatt cattaatatc atcactcctt taattatgtg tttctatatt aaaaaatatg
    12241 atttaaaatg agtacaacca attttgaatg gatttaccta tccaatttta aaagggaggg
    12301 agaagatggc taaatataaa gatattgcta gtgacataag agataaaata atcacagggg
    12361 attggtttta tggaatgaaa ataccttcac ataggcagtt ggcgatacag tacaacgtaa
    12421 atagagtaac gattattaaa agtattgagt tattagaagc tgaaggattt atctatacta
    12481 aagtaggtag tggaacatat gttaatgact atttgaatga agcacatatt acaaataagt
    12541 ggtctgaaat gatgttatgg tcctctcaac aaagaagtca gtatacggtg caattaatta
    12601 ataaaattga gacagatgat tcgtatatac atataagtaa aggtgaattg ggtatatcgt
    12661 taatgccaca tattcaattg aaaaaagcca tgtctaatac agccagtcat attgaagact
    12721 tatcttttgg ttataataat ggctatggtt atatcaagtt aagagatatt atcgttgaac
    12781 gaatgtcaaa gcaaggtata aatgtaggta gagaaaatgt aatgatcact tcaggcgctt
    12841 tacatgccat tcaactttta tctattgggt ttttaggtca agatgccata ataatttcga
    12901 atacaccatc atatattcac tctacaaatg tttttgagca attgaatttt agacatattg
    12961 atgttcctta taatcaaatt aatgaaattg ataccatcat tgatagattt attaatttta
    13021 aaaataaagc gatttatata gaacctaggt ttaataaccc gacaggtcgt tctttaacga
    13081 atgagcaaaa gaaaaatata attacttata gcgaaagaca taatattcct atcattgaag
    13141 atgatatctt tagagatatt ttctttagcg atccaactcc ttctatcaaa acttatgata
    13201 aattgggaaa agttatacat ataagcagtt tttcaaaaac gattgcacca gcaataagaa
    13261 taggttggat tgttgcttct gaaaaaataa tagagcaatt ggcagatgta agaatgcaaa
    13321 ttgactatgg atccagtatt ttgtcacaaa tggttgtata tgagatgttg aaaaataagt
    13381 cttatgataa acacttagta aagttaaggt atgttttaaa agataaacga gactttatgt
    13441 taaacatcct caataattta tttaaggata tagcacattg ggaggttcca agtggaggtt
    13501 attttgtatg gttagtcttt aaaatagata tagatattaa atatttattt tacgaattgt
    13561 taagtaaaga aaaaatatta atcaatccgg gttacattta tggcagtaaa gaaaagagta
    13621 taaggctatc ttttgccttt gaatcaaatg aaaatattaa gcatgcgctc tataaaattt
    13681 atacatatgt gaaaaaggtt taattaaaac aataattcga atcattatgt ggcatgttaa
    13741 acagctaaat ataagctatg cacatttaac aagcggatac tcctgacgca attatagtat
    13801 cattacatat tgtttaatcg tttggggtaa tgtaattaaa aggtaaaata ggcagagtgt
    13861 tatacataaa atgtaaagaa agaggcgata gaatgtctaa aatcaggtct tttacaatat
    13921 taagtctact tatttactta gctatgatgt gctatacagt agtgacctat ccaaaattac
    13981 caaccaaagt gcctattcat tataatttag caggggatgc tgataatttt gctgataaat
    14041 gggtgctgct tttgattaat agcgcattta tagtgatttg gcttatattt ttcattgcag
    14101 gtagatacta tgaacgattt gccaaatggt cacattataa tcatacacca cgtgaaattc
    14161 gagcgattaa attattttta agtacgttaa atttagagat tatgagctat atgtctatct
    14221 tcacagtatt agaaatttgg caaatacaac accatcatca attcaattta ctatggttta
    14281 atatgatatt tatcattatc attggtttga cgcttgtcat attttgttta cttcctacaa
    14341 ttcataaaat gagagattct caataaaaat ataaactctc atgatgcagt tttgaagcga
    14401 attcctgaga ttattataaa taatatctag tatttataat taaaatgata tattatttat
    14461 tatagttaaa tattgtgtat attttgtgaa ctttttgtaa aaattcgatt gcctgtcaca
    14521 tataggagtg ttacatttta aatatgtgat catcgcaaaa tataagttga aataggttgt
    14581 agattaatca gaatgataaa tattttatat aaagagaggg agtcattatg acactactta
    14641 ctgtaaatcc attcgataat gtcggattat cagccttagt tgcagcagta cctattattt
    14701 tatttttatt atgcttaacc gtttttaaaa tgaaaggcat ttatgcagca ttgacaactt
    14761 tggttgttac attgattgtg gctttatttg tatttgaatt accagcgcgt gtatcagcag
    14821 gtgcgattac agaaggcgtt gttgccggta ttttcccaat aggatatatc gttttaatgg
    14881 cagtttggtt atataaagtt tctattaaaa caggacaatt ttctattatt caagatagta
    14941 ttgcaagtat ttcagtggac caaagaatcc aactattatt aattggattt tgtttcaacg
    15001 catttttaga aggtgcagca ggatttggtg tgccaattgc gatttgtgca gtattattaa
    15061 ttcaacttgg atttgaacca ttaaaagcag cgatgttatg tttaattgct aatggtgcgg
    15121 cgggtgcctt tggtgcaatt ggtttaccag ttagtattat tgatacgttt aacttaagtg
    15181 gaggcgttac aacattagat gttgcgagat actcagcatt aacacttcca attttaaact
    15241 ttattattcc atttgtttta gtattcattg tagatggtat gaaaggtatt aaagaaattt
    15301 tacctgtcat tttaacagtg agtggtacat atactggatt acaattatta ttaacaatat
    15361 tccatggtcc agaactagca gacattattc catcactagc aacaatggtg gtgttagcat
    15421 ttgtttgtcg taaatttaaa ccgaaaaaca ttttcagatt ggaagcgtct gaacataaaa
    15481 ttcaaaaacg aacgcctaaa gaaattgtct ttgcttggag tccgttcgta attttaactg
    15541 cctttgtatt agtatggagt gcaccattct tcaaaaaatt attccaacct ggaggtgcac
    15601 ttgaaagttt agtaataaaa ttgccaattc caaatactgt gagtgattta tcgcctaaag
    15661 gaattgcgtt gcgtctcgat ttaattggtg caactgggac agcgatttta ttaacagtaa
    15721 ttattacaat tttaattacg aagttaaaat ggaaaagtgc aggtgcttta ttggtcgaag
    15781 caattaaaga attatggtta ccgatcctta caatttcagc tatcctagct attgctaaag
    15841 ttatgacata cggtggtttg actgtagcaa ttggacaagg tattgctaaa gcgggagcaa
    15901 ttttcccatt attctctcca gtattaggtt ggattggtgt gtttatgact ggttcagttg
    15961 taaataacaa tactttattc gcacctattc aagcgacagt ggcacaacaa atttcaacaa
    16021 gcggttcatt acttgtggca gctaacactg caggtggtgt agcagcgaaa cttatttcac
    16081 cacaatcaat tgccattgcg actgcagctg ttaaaaaagt tggtgaagaa tctgcattat
    16141 taaaaatgac gttaaaatac agtattatat ttgttgcttt tatttgtgtt tggacgttta
    16201 tactaacgtt aatattctaa atataaataa tgttgtcact tggattcaaa tgacatttta
    16261 aatctaatta ttcatgaatc gaactagtac gaaatgcaat gagcatcttg tctagttcga
    16321 ttttttaatg cctaaaaatg tcatatatgt aatcagagta gaaagtgttg aggcgtttca
    16381 gaagttgttt agaaaagtaa gtaaaataaa aaatgcactg agcaacaaaa gatgttgctc
    16441 gtgcatttag atgattctta tcatttcaaa taagaatgtg ttaatcaacg tatataagtt
    16501 aaaattggtt tggataaaat gatatctatc gttgtgtatt gtttgttttt atagttcgcg
    16561 acgacgtcca gctaataacg ctgcacctaa ggctaatgat aatccaccaa atacagttgt
    16621 accgatgaat ggattttctt caccagtttc tggtaatgct tgagctttgt tagcatctgc
    16681 atggtttgct ggttgcttct tatcaacaac aagttcttga ccaggtttga tcatgttttt
    16741 atcagctaat ttgttatctg cagcaatttt gtcagcagta gtgccgtttg cttttgcaat
    16801 gtcatttact gtatcaccag gtttaacgac atgtactccg ttaccatctt ctttaccagg
    16861 tttgttgcca tcttctttgc caggcttgtt gccgtcttct ttaccaggtt ttttgttgtc
    16921 ttctttacca ggcttgttgc catcttcttt accaggtttt ttgttgtctt ctttaccagg
    16981 cttgttgccg tcttctttgc caggcttgtt gttgtcttct ttaccaggct tgttgttgtc
    17041 ttctttgcca ggcttgttat tgtcttcttt gccaggcttg ttattgtctt cctcttttgg
    17101 tgcttgagca tcgtttagct ttttagcttc tgctaaaatt tctttgctca ctgaaggatc
    17161 gtctttaagg ctttggatga agccgttacg ttgttcttca gttaagttag gtaaatgtaa
    17221 aatttcatag aaagcatttt gttgttcttt gttgaatttg ttgtcagctt ttggtgcttg
    17281 agcatcattt agctttttag cttctgctaa aaggttagcg ctttggcttg ggtcatcttt
    17341 taggctttgg atgaaaccat tgcgttgttc ttcgtttaag ttaggtaaat gtaagatttc
    17401 atagaaagca ttttgttgtt ctttgttgaa tttgttatcc gctttcggtg cttgagattc
    17461 atttaacttt ttagcttctg acaataggtt agcactttgg cttgggtcat cttttaagct
    17521 ttggatgaaa ccattgcgtt gttcttcgtt taagttaggc atattcaaga tttcatagaa
    17581 agcattttgt tgttctttgt tgaaattgtt atcagctttc ggtgcttgag attcgtttaa
    17641 ttttttagct tcacctaaaa cgttagtgct ttggcttggg tcgtctttaa gactttgaat
    17701 gaagccgtta cgttgcgctt cgtttaagtt aggcatgttc aagatttcat agaaggcgct
    17761 ttgttgatct ttgttgaagt tattttgttg cgcatcagct tttggagctt gagagtcatt
    17821 aagtttttga gcttcaccta aaacgttagc actttggctt ggatcatctt taaggctttg
    17881 gataaaacca ttgcgttgat cagcatttaa gttaggcata tttaagactt gataaaaagc
    17941 attttgttga gcttcatcgt gttgcgcagc atttgcagca ggtgttacgc caccagatat
    18001 aagtaatgta cctaaagtta cagatgcaat acctacacct agtttacgaa ttgaataaat
    18061 gtttttcttt ttcaaattaa taccccctgt atgtatttgt aaagtcatca taatataacg
    18121 aattatgtat tgcaatacta aaatctatat ttataattaa atttaaaggt aagttttaca
    18181 acttataaaa taaatatttt gctgaaagat ttattcagga agtaaaaggc ttaaaaccgc
    18241 aaaatcacgc tatttcgat
//
LOCUS       DAKTGS010000047        17783 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.047, whole genome shotgun sequence.
ACCESSION   DAKTGS010000047 DAKTGS010000000
VERSION     DAKTGS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.047"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            73..2772
                     /gene="clfB"
                     /locus_tag="O0286_001315"
     CDS             73..2772
                     /gene="clfB"
                     /locus_tag="O0286_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCY0169699.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHK
                     TDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            2888..3649
                     /locus_tag="O0286_001316"
     CDS             2888..3649
                     /locus_tag="O0286_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0169700.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            3894..4349
                     /locus_tag="O0286_001317"
     CDS             3894..4349
                     /locus_tag="O0286_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0169701.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(4452..4562)
                     /locus_tag="O0286_001318"
     CDS             complement(4452..4562)
                     /locus_tag="O0286_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169702.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(4632..6056)
                     /locus_tag="O0286_001319"
     CDS             complement(4632..6056)
                     /locus_tag="O0286_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCY0169703.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6254..6454
                     /locus_tag="O0286_001320"
     CDS             6254..6454
                     /locus_tag="O0286_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169704.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            6480..7145
                     /gene="nsaR"
                     /locus_tag="O0286_001321"
     CDS             6480..7145
                     /gene="nsaR"
                     /locus_tag="O0286_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCY0169705.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            7156..8043
                     /gene="nsaS"
                     /locus_tag="O0286_001322"
     CDS             7156..8043
                     /gene="nsaS"
                     /locus_tag="O0286_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCY0169706.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            8151..8906
                     /locus_tag="O0286_001323"
     CDS             8151..8906
                     /locus_tag="O0286_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169707.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            8903..10903
                     /locus_tag="O0286_001324"
     CDS             8903..10903
                     /locus_tag="O0286_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169708.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            11562..11675
                     /locus_tag="O0286_001325"
     CDS             11562..11675
                     /locus_tag="O0286_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169709.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
     gene            12057..12317
                     /locus_tag="O0286_001326"
     CDS             12057..12317
                     /locus_tag="O0286_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169710.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            12327..12824
                     /locus_tag="O0286_001327"
     CDS             12327..12824
                     /locus_tag="O0286_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0169711.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(12932..13024)
                     /locus_tag="O0286_001328"
     CDS             complement(12932..13024)
                     /locus_tag="O0286_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169712.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            13219..15099
                     /locus_tag="O0286_001329"
     CDS             13219..15099
                     /locus_tag="O0286_001329"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCY0169713.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            15162..15767
                     /locus_tag="O0286_001330"
     CDS             15162..15767
                     /locus_tag="O0286_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCY0169714.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            15843..15938
                     /locus_tag="O0286_001331"
     CDS             15843..15938
                     /locus_tag="O0286_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169715.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            16260..17630
                     /locus_tag="O0286_001332"
     CDS             16260..17630
                     /locus_tag="O0286_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCY0169716.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
BASE COUNT         6380 a         2632 c         3224 g         5547 t
ORIGIN      
        1 aaagcaattg atataattct tatttcatta tacaatttag actaatctag aaattgaaat
       61 ggagtaatat ttttgaaaaa aagaattgat tatttgtcga ataagcagaa taagtattcg
      121 attagacgtt ttacagtagg taccacatca gtaatagtag gggcaactat actatttggg
      181 ataggcaatc atcaagcaca agcttcagaa caatcgaacg atacaacgca atcttcgaaa
      241 aataatgcaa gtgcagattc cgaaaaaaac aatatgatag aaacacctca attaaataca
      301 acggctaatg atacatctga tattagtgca aacacaaaca gtgcgaatgt agatagcaca
      361 acaaaaccaa tgtctacaca aacgagcaat accactacaa cagagccagc ttcaacaaat
      421 gaaacacctc aaccgacggc aattaaaaat caagcaactg ctgcaaaaat gcaagatcaa
      481 actgttcctc aagaagcaaa ttctcaagta gataataaaa caacgaatga tgctaatagc
      541 atagcaacaa acagtgagct taaaaattct caaacattag atttaccaca atcatcacca
      601 caaacgattt ccaatgcgca aggaactagt aaaccaagtg ttagaacgag agctgtacgt
      661 agtttagctg ttgctgaacc ggtagtaaat gctgctgatg ctaaaggtac aaatgtaaat
      721 gataaagtta cggcaagtaa tttcaagtta gaaaagacta catttgaccc taatcaaagt
      781 ggtaacacat ttatggcggc aaattttaca gtgacagata aagtgaaatc aggggattat
      841 tttacagcga agttaccaga tagtttaact ggtaatggag acgtggatta ttctaattca
      901 aataatacga tgccaattgc agacattaaa agtacgaatg gcgatgttgt agctaaagca
      961 acatatgata tcttgactaa gacgtataca tttgtcttta cagattatgt aaataataaa
     1021 gaaaatatta acggacaatt ttcattacct ttatttacag accgagcaaa ggcacctaaa
     1081 tcaggaacat atgatgcgaa tattaatatt gcggatgaaa tgtttaataa taaaattact
     1141 tataactata gttcgccaat tgcaggaatt gataaaccaa atggcgcgaa catttcttct
     1201 caaattattg gtgtagatac agcttcaggt caaaacacat acaagcaaac agtatttgtt
     1261 aaccctaagc aacgagtttt aggtaatacg tgggtgtata ttaaaggcta ccaagataaa
     1321 atcgaagaaa gtagcggtaa agtaagtgct acagatacaa aactgagaat ttttgaagtg
     1381 aatgatatat ctaaattatc agatagctac tatgcagatc caaatgactc taaccttaaa
     1441 gaagtaacag accaatttaa aaatagaatc tattatgagc atccaaatgt agctagtatt
     1501 aaatttggtg atattactaa aacatatgta gtattagtag aagggcatta cgacaataca
     1561 ggtaagaact taaaaactca ggttattcaa gaaaatgttg atcctgtaac aaatagagac
     1621 tacagtattt tcggttggaa taatgagaat gttgtacgtt atggtggtgg aagtgctgat
     1681 ggtgattcag cagtaaatcc gaaagaccca actccagggc cgccggttga cccagaacca
     1741 agtccagacc cagaaccgga accaagccca gacccggatc cggattcgga ttcagacagt
     1801 gactcaggct cagacagcga ctcaggttca gatagcgact cagaatcaga tagcgattcg
     1861 gattcagaca gtgattcaga ttcagacagc gactcagaat cagatagcga ttcagaatca
     1921 gatagcgact cagattcaga tagcgattca gattcagata gcgattcaga ttcagatagc
     1981 gattcggatt cagacagtga ttcagattca gacagcgact cagaatcaga tagcgactca
     2041 gaatcagata gtgagtcaga ttcagacagt gactcggact cagacagtga ttcagactca
     2101 gatagcgatt cagactcaga tagcgattca gactcagaca gcgattcaga ttcagacagc
     2161 gactcagatt cagacagcga ctcagactca gacagcgact cagaatcaga tagcgattca
     2221 gactcagaca gcgactcaga ctcagacagc gactcagact cagatagcga ctcagattca
     2281 gatagcgatt cagactcaga cagcgactca gattcagata gcgattcgga ctcagacagc
     2341 gattcagatt cagacagcga ctcagactcg gatagcgatt cagattcaga tagcgattcg
     2401 gattcagaca gtgattcaga ttcagacagc gactcagact cggatagcga ctcagactca
     2461 gacagcgatt cagactcaga tagcgactca gactcggata gcgactcgga ttcagatagc
     2521 gactcagact cagatagtga ctccgattca agagttacac caccaaataa tgaacagaaa
     2581 gcaccatcaa atcctaaagg tgaagtaaac cattctaata aggtatcaaa acaacacaaa
     2641 actgatgctt taccagaaac aggagataag agcgaaaaca caaatgcaac tttatttggt
     2701 gcaatgatgg cattattagg atcattacta ttgtttagaa aacgcaagca agatcataaa
     2761 gaaaaagcgt aaatactttt ttaggccgaa tacatttgta ttcggttttt ttgttgaaaa
     2821 tgattttaaa gtgaattgat taagcgtaaa atgttgataa agtagaatta gaaaggggtc
     2881 atgacgtatg gcttatattt cattaaacta tcattcacca acaattggta tgcatcaaaa
     2941 tttgacagtc attttaccgg aagatcaaag cttctttaat agcgatacaa ctgttaaacc
     3001 attaaaaact ttaatgttgt tacatggatt atcaagtgat gaaacgacat atatgagata
     3061 tacaagcata gaaaggtatg cgaatgaaca caaattagct gtgattatgc ccaatgtgga
     3121 tcatagcgca tatgctaaca tggcatatgg tcatagctat tatgattata ttttggaagt
     3181 gtatgattat gttcatcaaa tatttccact ttccaaaaag cgtgatgaca attttatagc
     3241 aggtcactct atgggaggat atggcacaat taaatttgca ttaacacaag gggataaatt
     3301 tgccaaagct gtaccattat ctgctgtgtt tgaagcgcaa aatttaatgg atctagagtg
     3361 gaatgatttt tcaaaagagg ccataattgg caatctttca agtgttaaag gaactgaaca
     3421 tgatccgtat tacttgctag acaaagctgt agctgaagat aaacaaattc caaaattgct
     3481 cattatgtgt ggtaaacaag actttttata tcaagacaac ttagatttta tcgattattt
     3541 atcacgcata aatgttcctt atcaatttga agatggacca ggagatcatg attatgcata
     3601 ttgggatcaa gcgattaagc gtgctataac atggatggtg aatgattaat tatttcttgg
     3661 aaaatatgtg gctgcattaa atacacagag tgagagatac aaactattta cgcacgacta
     3721 acatttctaa gtgtttaaat tatttttgta ttaatatgat tggcgcaatt tgctgataca
     3781 caaaaatgtt tctcgtgaaa cttagattta gcttatagtt ttatcatcat ttgtatgact
     3841 tacattataa attttattat aatgaggtta acgctttgaa aggagtcatc atcatgtcga
     3901 ccaataaaaa cgattatgag catatgttgt tttattttgc atataaaacc tttattacta
     3961 ccgctgatga aattatagag aagtatggta tgagtcgtca gcatcatcgt tttttgtttt
     4021 ttatcaataa attacctggt attactatta aatcattact agaaatatta gaaatttcta
     4081 aacaaggatc acatgcaaca cttcaaaaat taaaagagca aggtctcatt attgaaaaag
     4141 ttttagagac tgatcgacgt gtcaaaaaat tatattcgac ggataaaggc gatcaactca
     4201 ttgctgaatt gaacaaggcg caagatgaat tattgcaaaa tatatatcaa caagtcggtt
     4261 cggattggta tgatgtgatg gaagcattgg ctaaagggcg acctggcttt gattttatta
     4321 agcatttgaa agatgaaaaa gaaagctagc atcagaaatg ttaaaaatct tcgcattctt
     4381 aaatttaaaa aatatgtcaa aaagtgtata ataaaaacat ataatttaat tgaactcagt
     4441 ttcaacacat cttagaaagg agtttgaatg atgaaaaaat tagcagttat tttaacatta
     4501 gttggcggtt tatacttcgc atttaaaaaa taccaagaac gtgttaacca agcacctaac
     4561 attgagtact aaattaaacc ataaaaaatt cccgaacacc ttgttatagt gctcgggaat
     4621 ttttttatgc tttacttgaa tatatcaaat attatttttg cgctttctgt attttcgata
     4681 ttaccactaa atgattctga tctaggtccg taagcgtagg tattaacatc ctcgcctgta
     4741 tgtccatcgg aagtccaccc tgtataagat ttatcattta ctggcttctg aatagcgtgt
     4801 tgtagggctt ttgtttgcgt ttctacttct gcggattttt cgtctttttc ttttttaagt
     4861 agtcttttta gctttttatt ctctttttta acctttttca tatcatcttg tgaaaattca
     4921 aatccataac cttcattaat aactttttca gggtcttcac ctttagccat tttttctgtc
     4981 atatatgatc cagagtgttt catagattta atcggttgag gattccattc gtatccttta
     5041 tctttaccaa ttgttagacc accagtagag tgatctgctg ttgcaacgac aagtgtatct
     5101 ttatgtttct tagcatattg aatggcgtca tcgaatgctt tttcaaaacc ttccatttca
     5161 gacataacgc ctgtaatatc gttggaatgc gctgatttat ctatagaagc accttcgacc
     5221 attaaaaaga atcctttttt attgcgctca agcttactaa gtgcactttg ttgcatatca
     5281 gctaatgatg gttcgtcttt agaagcatct attgcaagtg gcatattttt atctgcaaac
     5341 aaaccaagaa ctttatcttt atcagatttt gataactcct tactgttcgt ggcaaggtcg
     5401 taaccatctt ttttgaattt tttatctaaa ttgccattac ttttaccgaa atatttagcg
     5461 ccgccgccta ataaaacatc aactttatgc tttccgttga ttttatcttt ataaaattgt
     5521 ttagcgattt cgtttttatc atctctagaa gtcacgtgtg cagcatatgc tgctggtgtt
     5581 gcatctgtta attcagctgt tgaaacaaga ccagtcgact tacctttttc ttttgcacgt
     5641 tcaagcaccg tctttacttt ctgcttgtta ctgtcaacac cgatggcacc attatatgtc
     5701 ttatgaccag aactaaaggc tgttccgcca gctgcagaat cagtaatatt ctgttttggg
     5761 tcatttgaat atgtacgatt tgtgcctttt aaatatgaat caaaagcagt aggggtcatt
     5821 tctttagcat gcggatcatt tttataataa cgataagctg tgttaaatga tggacccatg
     5881 ccatcgccaa ctaaaaagat aacatttttt ggatttttag tattaccaac cgcgaaactt
     5941 tcatctttag aacttttatc ggattgcgca attgcaggtg tgacagaact aaaaaccgtt
     6001 gacacgataa taaggttagc aactgcaaat tttgtggctt ttttaactga taacataaga
     6061 catcctcctg agtatatgac tatgtcttca gtgtaaaaga ggaattttga gcaattatgt
     6121 agttttagtt aaaaatatgt aaacagagtg atttagaata acaaaaaatg aatatatatg
     6181 acaatttgtt atagaaagcg ttagaataga agcgtgtgaa aatatagaat taaatataat
     6241 ttgaggtgga aaaatgatac tagtaatgtt atctccatta ttaatcatat tctttatagt
     6301 gttgtctatt ttagaagagc gtaaacgtac gaagaaaaag caactcgaga aagaaaaagc
     6361 aaatacacta aatcaaaata caaatgacac ggaaagttca aatcaagagc cgtcattgca
     6421 gcaggataaa gaacaaaaag ataacaaagg ataattcaat tgaaggaaga agattataga
     6481 tgaaaatatt aattgttgaa gatgattttg ttatagcaga gagtttagca tctgaactta
     6541 aaaaatggaa ttacggtgtt attgtcgttg aacaatttga tgatatactg tctatcttta
     6601 accaaaatca acctcagctt gtattgctag atattaattt gccaacgtta aatggttttc
     6661 attggtgtca agaaatccga aaaacatcta atgtgccaat tatatttatt agttcccgta
     6721 ttgataatat ggaccaaatt atggcaatac aaatgggggg agatgatttt atcgaaaagc
     6781 catttaactt gtcattaacg attgccaaaa ttcaagcatt attgagacga acttatgact
     6841 tgtcagtagc taatgattca ttgacggtta aaggttgtac attaatatta gatgaagcaa
     6901 aagtcgtgta tcaagaacaa aacatacagc tatctttgac tgaattacaa atattaaagt
     6961 tattatttca aaatgaagat aaatatgtaa gtagaactgc tttaattgaa aaatgttggg
     7021 aatcagaaaa cttcatagat gataacacat tagctgttaa catgacgcgc ctgcgtaaaa
     7081 aattaaatac tattggcgtt aatgatttta tcattacaaa gaaaaatgtc ggatataaag
     7141 tatagggtga atgcaatgac ctttcttaaa agtattactc aggaaatagc aatagtcata
     7201 gttatttttg ctttgtttgg cttaatgttt tacctgtatc atttgccatt agaagcatat
     7261 ttactagcac ttggcgttat tttattatta ttactcatat tcataggtat taaatattta
     7321 agttttgtaa aaactataag ccaacaacaa caaattgaaa acttagaaaa tgcgttgtat
     7381 cagcttaaaa atgaacaaat tgaatataaa aatgatgtag agagctactt tttaacatgg
     7441 gtacatcaaa tgaaaacacc cattactgca gcacaactgt tacttgaaag agatgagcct
     7501 aatgttgtta atcgtgttcg tcaagaggtt attcaaattg ataactatac aagtttagca
     7561 cttagttatt taaagttatt aaatgaaact tctgatattt ctgtcactaa aatttcgatt
     7621 aataatatca ttcgcccaat tattatgaaa tattcaatac agtttattga tcaaaaaaca
     7681 aaaatccatt atgaaccttg tcatcacgaa gtattaactg acgttagatg gacctcttta
     7741 atgatagaac aattaataaa taatgcactt aagtatgcga gaggtaaaga tatatggatt
     7801 gaatttgatg agcaatccaa tcaattacac gtaaaagata atggtatcgg tattagtgaa
     7861 gcggacttgc ctaaaatatt tgataagggc tattcaggtt ataatggcca gcgccaaagt
     7921 aactcaagtg ggattggttt atttatcgta aaacaaattt caacacacac aaaccatcct
     7981 gtttcagtcg tatctaaaca aaatgagggt acaacattta cgattcaatt tccagatgaa
     8041 taaaaacttt caatattgta agtatactag taacattttt ttactaattt aaatgttatt
     8101 agtatttttt tgttttaata tagaactaac aaagaaatga ggtgcatgcc atgttgctag
     8161 aagtgaaaca tgtaaaaaag gtttatggta aaggtttgaa tgctacgaca gcacttaatc
     8221 aaatgaattt atcagttgga gctggtgaat ttgttgcaat tatgggtgag tctgggtcag
     8281 ggaagtctac actactaaat ttaattgctt cttttgatgg actaactgaa ggtgacatta
     8341 ttgtggatgg cgcacattta aataatatga aaaataaaag taaagcattg tatcgtcaac
     8401 aaatggtagg ttttgttttt caagatttta atcttttacc aacaatgacg aataaagaaa
     8461 atataatgat gccattaatt ttagctggtg ctaaacgaaa agatatagaa caaagggtac
     8521 atcagttggc agtacaatta catttagagg gattcttaaa caagtatcct tctgaaatct
     8581 ctgggggtca gaagcaacgc attgccattg cacgtgcatt agttactaag ccgacgattt
     8641 tactagccga tgaacctaca ggtgcacttg attcaaaaac atcaaaggca ttgatgatgt
     8701 tatttcaaga gattcatcaa ttggaacaga caattttaat ggtaactcat tcaaatatcg
     8761 atgcgtctta tgcagagcga gtcattttta ttaaagatgg gcgtctatat catgaaatat
     8821 atcgtggtga agaaagtcaa ttagcttttc aacaacgaat aacagatagc ttagcacttg
     8881 tgaatggagg aagtgtcaat atatgaagtt aagattgtta tggcacatag tgcgacgtca
     8941 atttattacg cagcgacttg taatcattcc attcatttta gcggtaagtg tactattcat
     9001 gattgaatat acgcttgtgt caattgggtt aaatagctac ataaaacaga agaatgactt
     9061 cctagtacca tttattatca tagctaattt ttttatggcg cttttaactt ttatttttat
     9121 tttctatgca aatcacttta tgatgtcaca aagacgaaaa gagtttagca tttttatgac
     9181 attgggcatg accaagaaaa gtatgcgttt aattgtagtg atggaaacta tcttacaatt
     9241 tgtgataatt tcagtcgtta gtattgccgg cggatactta cttggtgcga tatttttctt
     9301 gtttatacag aaaataatgg gcagtgaagt tgcgacgtta aggtattatc catttgactc
     9361 tgtagcgatg tttattactt tgattatcat tgctgtatta atgggcatgc tacttatatt
     9421 caacttgttt agtattaatt ttcaacggcc gataacttat caacatcgtt ccgattctag
     9481 tgtcatatca cgatggttgc gttacgtttt aattgttata ggaagcgcag cactatattt
     9541 aggttacttt attgcattac aacaagatac gacgtttggt gcctttttta aaatatggat
     9601 tgtcatagga ttagttatta tcggtactta tgcatttttt gtaggtataa gtgaaataat
     9661 tattagtata ttgcagcagg tatcaaaagt ttactatcat ccacggtatt tttttgtggt
     9721 agttgggatg cgtgtacgtc ttaaaatgaa tgcagtcagt cttgcaacaa tcactttgct
     9781 gtgtacattt ttgattgtaa cgctcacaat gacattaaca acctatcgtg atatgaatca
     9841 taccattacg aaattgatta cgaatgatta tgatttgtca tttagcgaca attctaagtc
     9901 acaaatagaa cgtcaacaaa caattgagaa tattaaacgt gatatacagg gttcggttaa
     9961 tgcaaaagac ttcaaagtat atgaaacgac attatttaga gcttctttag aaaagaatcg
    10021 tgcttattac aagttaaaac aggctagtga tgatattcca atagacttca ttggtaatga
    10081 aggcgcgata ttttcaagac aatcagtaat gattacagct tttactgcac aagattataa
    10141 tcgatatcac caaaacaaat tacacttaga caatcaaagt ataggtatga ttacgaatgt
    10201 acctattttt aaaaagcagt ctaaggtcgg tcttaataat gaagtcttta aagtgaaaca
    10261 acttgacgca catgcgttaa atttgatgat gattcaagat agtatggttt taattgttaa
    10321 agataacaat caattacaaa aagtaaaagg attttatgca catgaacaaa agaattcgac
    10381 aatatcgata aactttaatg tatttggaaa gacgaaatta acaacatcac aaatacaaaa
    10441 gctatcgaaa aaatacgatg cgtcaataaa ttctaaatca gaaatgctaa tggtttggga
    10501 tcgtttgaca ggtggactaa tatttgtagg tggcgtagta tcaattgtac ttgtcattgg
    10561 aatatttcta atgatgtatt ataaacaagt ctcagaagcc tatgcaaatc agcacaatta
    10621 tgacattatg aaaaaacttg gtttagataa tggaagaata gcaaagataa cacgaaatca
    10681 aatgactttc ttgttcgcaa taccaataac agtagcactg attcacacat taatttctag
    10741 taacattgta tatacgttac taaatatgat aggtattaat aaccatcata tatttttaac
    10801 aagttacgta ctagctgtca ttataatttc gtttctatat atggcgatgt acaaaataac
    10861 ttcctatatt tatgctaaag ttattcatca acaacgcaat taaccatgtt tcagtgaaat
    10921 cacaatcaat catgtatttt cactgaaata tattatatta aaaagaaacc taaaaacatt
    10981 acaaataaca ataacaaatt tcaaccgata agataaaatg tcttgtcggt ttttaatata
    11041 acaagtgata ttttggaaat gccaaaaata tatagattgt ttatataaaa tgatttaaaa
    11101 tgttttataa caacatattt tatgcaacac ataaaatggg taactaacat cacgaaatca
    11161 ttgtatttgt tgttacactg atgaagcgat gtcacaaaga aatgtcgtta tcaaacgaag
    11221 cccgaaatac atactgatac tcgaaataat gtaacgataa ataacaatga taacgattga
    11281 ttactacgca aaaatatatg taaattgacg atttaaatgc aattcgtata ttttgccaat
    11341 gatttacaca ttaaaatgtt tgaaaattgt cacaattcag tctaatttaa aaggaagtta
    11401 attacaaaat taaaaaatca tgcatcgtgt catttagata ttgaaaaaga tgagtcaaat
    11461 ttgtttcata tattttccaa taacaaatta ttatatctaa atatacaatc agaaatatat
    11521 gaatagaaat atcaataaga aaaataatat gattaaaaat gatgaatggc atacttataa
    11581 agtgtctaaa tattggcggt caatattact tacaaacacg aatgttaagt aatgtagaca
    11641 atgctgaaaa aataataagt agaaactcta tatagaatgt tcgtttttat ttttaataat
    11701 tgaataatca ttattgatga tttaatatgt attcggtcgc acacgataca aagtgacttg
    11761 ttttaatgat atgcaataat atgacgttta tcgtcgcgtg taacaatgta tgagtgtgtc
    11821 agtttaattt taaaagggtt ataaggatat ttaataagat aaaggagggc ttaataaaca
    11881 atcacatgtg aagtttttaa ggattatatg agttgggcag ctctttaatc catagttata
    11941 acgaatttta aattgttaga tctaacgtat ggtatcagta tgtatgagtt taacttaagt
    12001 attaatctat agtttaaaga gcggtctaat tgtcttttta aaaatagaaa ataaggatga
    12061 ttataatgag gggttataac ggaagatatt gtagctggac taatacagct atacaatgtg
    12121 attattttaa taaagtaaga caggtgaaaa aagacggtaa atcggatagg ttgagcaaca
    12181 acataaactc gaacattatt tatcgtgtgc tcgaaaaatg tatcacgcta tataatgtgt
    12241 gttcacggtg taaacgcgca cttattttat cagaacattc taaaaagaac atattcaata
    12301 taaaaaaggc tgtgtagggg tgtcttatga ataatagaaa tgtgtatgat atcgaagtaa
    12361 gtgattataa aggcttaact tataaattag aagcatttag aggtaaagtg attttagttg
    12421 ttaatactgc aacagaatgt atatatagcg aacaattgaa aaaactagag actttatttc
    12481 aaaaatataa ggatcgtggg tttgtagtgt tgagttttcc gaataataat tttgacaatc
    12541 gacaaccagg ttctaatgaa gaaatcttga aaattagtcg tgagaaattt ggtgtaacat
    12601 ttccagtgtt agctaaaata tctgtgaacg ggaacgatga acatccgtta tttacgcatt
    12661 taaagaatga acaaccagga atctttgggt ccccaataaa atggaatttc acaaaattta
    12721 taatcgatcg acaaggaaat gttgtaaatc gattcttgcc aatggaagat ccattggata
    12781 tatcgacaaa tatagaaata ttattgcaag aatcttcatc ttaaatttaa atttgagcgc
    12841 ttagtatgca aataacacaa gagcctctta atgatgtact aatagtatgt tattaggagg
    12901 cattttatat ttagaaaatc agtaatttga cctattttac tgaaaggctg atttggtaca
    12961 taataaatag taaataaata aaaaataaca acgatgcatt actgtatgca ttaataactg
    13021 acacgacata atcaaagttt tgtctgaatt actgataatg tcattcaagc aacatgaagg
    13081 ttgtaaatat ttgcaagtat aactatcaga cacaactcaa gtttgtttta atttatacca
    13141 tacatattaa aaaattgact aaactatcga taaaaaaaga gtcaatcatc tatttcaagt
    13201 ttattgaggt gaaaacattt gaaattaaat acatctaata gtccatttac agaaaaacaa
    13261 gtcacagaga ttaacaatct gctgcaaaca ttaacagaga gccaacaaca gtggcttagt
    13321 ggttttttac tagctaatag taacgatacg acaagtgata gtaatcaaca acaattagag
    13381 acagaagtgt ggcaacaatc acaaatatca gaagaacaag caacttcaac aacgtatatg
    13441 ttacaaaata aagagccaca tatcgaagct aatcagcggc atgttacagt gctatatggt
    13501 tctgaatcag gtaatgccat gcgtttagct gaaatttttt cagaacgttt aagtgatatc
    13561 ggacatcaag ttgttttgat gtcaatggat gaatatgata cgacaaacat cgcgcagtta
    13621 gaagatttat ttattattac gtctactcat ggtgaaggag aaccgcctga taatgcatgg
    13681 gatttctttg aatttttaga agacgataac gcacctaatt taaatcatgt gagatattca
    13741 gtactagctt taggtgatca aacatatgaa tttttctgtc aagccggtaa agatgtagat
    13801 gttttactag aaaatctagg cgctgagcgt atatgtaagc gtgtagattg tgatattgat
    13861 tatgaagaag acgcagaaaa gtggatggca gacatcatta atattattga taccacatca
    13921 gaaggtattc aaagtgaatc ggtgataagt gaatcaatta agtctgccaa agaaaagaaa
    13981 tattctaaat caaatccata ccaagcagaa gtattagcga atatcaattt aaatggtacc
    14041 gattcaaata aagaaacacg acatatagaa tttttacttg atgattttag tgaatcatat
    14101 gaaccaggag attgtatagt agcattaccg caaaacgacc ctgaattggt tgaaaaacta
    14161 atattcatgt taggttggga tccgcaatct ccggtgccaa ttaatgatca tggtgataca
    14221 gttcctattg ttgaagcact aacatcacat tttgaattta ctaaattaac attgccatta
    14281 ttgaaaaatg cagatatcta ttttgacaat gaagaattat ctgaacgtat tcaagatgag
    14341 tcatgggcgc gtgaatatgt tataaatcgg gactttatag atttaataac agattttcca
    14401 actatagaat tacaacctga gaatatgtat caaatcctta gaaaattacc accaagagag
    14461 tattcgattt ctagtagttt tatggcaacg ccagatgaag tgcatattac cgttggtacg
    14521 gttcgttatc aagcacatgg acgtgagaga aaaggtgtat gctcggttca ttttgctgag
    14581 cgaattaaac caggcgatat agtaccaatt tatttgaaga aaaatccgaa cttcaaattt
    14641 ccgatgaagc aagatatacc ggttattatg attggaccag gtactggaat tgctcctttt
    14701 agagcatatt tacaagaacg tgaagaactt ggtatgactg gaaaaacatg gttgttcttt
    14761 ggtgatcaac accgtagttc tgacttttta tatgaagaag aaatagaaga atggcttgaa
    14821 aatggaaact taacacgcgt agatttagca ttttcaagag accaagaaca caaagaatat
    14881 gtacagcatc gtataatgga agaaagtaaa cgtttcaatg aatggattga gcaaggcgca
    14941 gcaatctata tttgtggcga tgaaaaatgt atggcgaaag atgtccatca agccattaaa
    15001 gatgtattgg taaaagaacg tcatatttct caagaagaag cagagttatt attgcgacaa
    15061 atgaaacaac aacaacgcta tcaacgtgat gtttattagc gattggtgtt aaatatttta
    15121 aggtgtaatg atgtaaaaag atataaagga tgttgctcaa catgaatatg ccattaatga
    15181 tagatttaac aaataaaaat gtcgtcatag ttggtggagg cgtcgttgca agtcgtcggg
    15241 cacaaacatt aaatcaatac gttgaacata tgacggtcat cagtccgaca atcactgaaa
    15301 aacttcaaaa tatggtagat aacggtgtcg tcatatggaa agaaaaagaa tttgaaccaa
    15361 gcgatattgt agacgcgtat ctagttattg cagcaaccaa tgagccacgt gtcaatgaag
    15421 cggtaaaaaa agccttacct gagcatgccc tttttaataa tgttggagat gcatcaaatg
    15481 gcaatgttgt atttccaagt gcactacacc gcgacaagct aactatcagt gtatcaactg
    15541 atggtgcgag tcctaagttg acaaaatcaa ttatggcaga gcttgaggcg ttatatccac
    15601 catcatacag ttcgtatatc gactttttat atacttgccg acagaaaata aaagtacttg
    15661 atataacata taacgaaaag caacagttac tgtcacaaat tgtgtcacaa gaatatttaa
    15721 atcatgacaa acaagctcaa tttttagcgt ggttggatgt aagataataa tagcggaccg
    15781 tctaaccgtc taaggtaagt cttcttattt taactttaac gcttaatcat tgaaattaag
    15841 acatgggcgg ctttgtgaat agtctaataa tgaaggattt aagcgataat gatatgcgtt
    15901 ttaaatatga atattacaat agagaaaaag atacgtagaa caaacttaat aaaataggtg
    15961 gataaattga aatctggttg aagtcgttac tatcatagcg acctttagcc agattttttg
    16021 tgcaatagaa agcaataata aaaatgatag atcaaaatga aatacaggac aggatataca
    16081 aggattagtc atgccatgtt atcaagtagg aaaatcaaac ttcactattg atagttacgc
    16141 aaaaaagatt tttttgataa aatgagataa cttaaatata aaaaattata ttaattataa
    16201 tatttaagtt aaagaggggg attatgtaaa ttgtattaaa agtggaggga gaaaataata
    16261 tgaatagtga taatatgtgg ttaacagtaa tggggctcat tattattatt tcaattgtag
    16321 gtttactcat tgccaaaaag ataaatccag ttgtaggtat gacaatcata ccttgcttag
    16381 gggcaatgat tttaggatat agtgtgacag atttggttgg attttttgct aaagggttag
    16441 atcaagtcat caacgttgtt attatgttta tctttgccat tattttcttt ggcatcatga
    16501 acgatagtgg tttattcaag ccgcttgtca aacgcttaat attaatgaca cgaggcaatg
    16561 tcgtcattgt ctgtgcaatg acagctttaa ttggcacaat agcccaatta gatggggccg
    16621 gtgcggtaac atttttgctt tctattcctg cattattacc tttatataaa gcgttaaata
    16681 tgaataaata tttattgatt ttactattag cattaagcgc ggcgattatg aacatggtac
    16741 cttggggagg tccaatggct cgtgtagctg cagtgttaaa agccaaaagt gtcaatgaat
    16801 tatggtatgg attaatacct attcaaataa taggtttcat tcttgttatg ttgtttgcgg
    16861 tatatcttgg atttaaagaa cagaaacgta tcaaaaaagc aatagagaga aatgaattac
    16921 cgcaaacaca agatatagat gtacataaat tagttgaagt atatgaacga gatcaagatg
    16981 taaggtttcc tgtaaaagga cgtgcaagaa caaaatcatg gataaaatgg gtgaatacag
    17041 ctttaacttt agctgttatt ctatcgatgt taataaatat tgcgccacct gaatttgcat
    17101 tcatgatagg tgtttcgttg gcacttgtta ttaattttaa atcagtggat gaacaaatgg
    17161 aacgattaag agcgcatgcg ccgaatgcat taatgatggc tgcagtgatt attgcagcag
    17221 gtatgttttt aggtgtacta aatgaaaccg gtatgcttaa agcgattgcg accaatttaa
    17281 tcaaagtgat tcctgcagaa gtaggaccat acttgcatat tattgtaggt ttacttggcg
    17341 taccattaga tttactaact agcacagacg cttattattt tgctgtgtta ccgattgtcg
    17401 aacaaacagc agggcaattt ggtgtaccgt ctgtatcaac agcttattca atggtcatag
    17461 ggaatattat aggtacattt gtcagcccat tttcaccagc cttatggttg gcaattggtt
    17521 tagcagaggc aaacatgggc acgtatatta agtatgcatt cttttggatt tggggattcg
    17581 ctatcgttat gttagtaatt gcaatgttga tgggcattgt gacgatttaa gtatgaaaaa
    17641 atagaaacta tggtcacgtt gcaaaatgaa ataatagttg cataaacatg tcgaaatgac
    17701 ggacgaatct ttaaacaatt ttaaaaatta atgaaataat tgtgtagaaa tatgaatttc
    17761 actaaatgtt aataactttg tga
//
LOCUS       DAKTGS010000048        17562 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.048, whole genome shotgun sequence.
ACCESSION   DAKTGS010000048 DAKTGS010000000
VERSION     DAKTGS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.048"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            143..811
                     /locus_tag="O0286_001333"
     CDS             143..811
                     /locus_tag="O0286_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0169717.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            813..3290
                     /locus_tag="O0286_001334"
     CDS             813..3290
                     /locus_tag="O0286_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCY0169718.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            3633..6263
                     /gene="alaS"
                     /locus_tag="O0286_001335"
     CDS             3633..6263
                     /gene="alaS"
                     /locus_tag="O0286_001335"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCY0169719.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            6326..6586
                     /locus_tag="O0286_001336"
     CDS             6326..6586
                     /locus_tag="O0286_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCY0169720.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            6590..7018
                     /gene="ruvX"
                     /locus_tag="O0286_001337"
     CDS             6590..7018
                     /gene="ruvX"
                     /locus_tag="O0286_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCY0169721.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            7033..7341
                     /locus_tag="O0286_001338"
     CDS             7033..7341
                     /locus_tag="O0286_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCY0169722.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            7626..8264
                     /locus_tag="O0286_001339"
     CDS             7626..8264
                     /locus_tag="O0286_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCY0169723.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            8267..9190
                     /locus_tag="O0286_001340"
     CDS             8267..9190
                     /locus_tag="O0286_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0169724.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            9202..10470
                     /locus_tag="O0286_001341"
     CDS             9202..10470
                     /locus_tag="O0286_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0169725.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            10470..11093
                     /gene="udk"
                     /locus_tag="O0286_001342"
     CDS             10470..11093
                     /gene="udk"
                     /locus_tag="O0286_001342"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCY0169726.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            11121..11597
                     /gene="greA"
                     /locus_tag="O0286_001343"
     CDS             11121..11597
                     /gene="greA"
                     /locus_tag="O0286_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCY0169727.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            11923..12657
                     /gene="pxpB"
                     /locus_tag="O0286_001344"
     CDS             11923..12657
                     /gene="pxpB"
                     /locus_tag="O0286_001344"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCY0169728.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            12647..13657
                     /locus_tag="O0286_001345"
     CDS             12647..13657
                     /locus_tag="O0286_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0169729.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            13659..14108
                     /locus_tag="O0286_001346"
     CDS             13659..14108
                     /locus_tag="O0286_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCY0169730.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            14122..15483
                     /locus_tag="O0286_001347"
     CDS             14122..15483
                     /locus_tag="O0286_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0169731.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            15483..16235
                     /gene="pxpA"
                     /locus_tag="O0286_001348"
     CDS             15483..16235
                     /gene="pxpA"
                     /locus_tag="O0286_001348"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCY0169732.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            16248..17477
                     /locus_tag="O0286_001349"
     CDS             16248..17477
                     /locus_tag="O0286_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCY0169733.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
BASE COUNT         6387 a         2395 c         3452 g         5328 t
ORIGIN      
        1 ttaatggttc ttaagcattt ctattccatt cccgcttttt catgaatgaa gatgatatta
       61 gattatattt tattcgttgt taagtgattc gagacataca atttatcaag atgttataat
      121 tgatgagaaa tgaggttcgt aaatgataga tcaacaaaca atttatcaat acatacaaaa
      181 tggaaaaata gaagaagcgt tacaagcatt gttcggaaat atcgaagaaa atcctacaat
      241 tattgaaaat tatattaatg ctggtatcgt acttgctgat gcgaatgaga ttgaaaaggc
      301 agagcgtttt ttccaaaaag ctttaacaat agatccgaag aatggcgtcg tattttttaa
      361 tctagcaaat gtatattata atcagcaacg ttatcaagaa gctattaaat tatatcaaca
      421 agcattacaa acagagattg aacaagttga ttgtaattat atgatcggta tggcgtttaa
      481 tcagttagaa tcatttaagc tggcattgcc gtatttaatg actgctgcgg aactagataa
      541 agacaaagat gcagaagttc aatttcaata tggtcttgta ttatgtcaat tagaaatgtt
      601 taatgaagcc ataactcaac ttaaacatgt attaacgatt gataaaaatc atgttgatgc
      661 aagatacaat ttgggcttag cgttatttat gaaaaatgaa gatattgatg aagcaataac
      721 tcattttaaa gaagctgtga ctatcgaccc taaacactta ttaagtcagc atgcgctgaa
      781 aacattcact aaaatgaaag aggaggagta acatgtcaga ccctacacta tttgattatt
      841 caatgatcaa aggtactgtt gaagcgatat tgtttcaaaa cagtgataat ttttatacag
      901 tgcttaaagt agatactatt gaaacaaatg aagactttga tacaatgcca actgttgtag
      961 ggtttcttcc gaatattgtt gaaggcgatg tctacacatt taaagggcaa gttgttgatc
     1021 atccacgtta tggtaaacaa ttaaaagcag agacttttga aaaagaaatg ccacaaacga
     1081 aagaagcaat tataagttat ttatctagtg atttgtttaa aggtgtaggt aaaaagacag
     1141 ctcaaaatat tgttaataca ttaggtgaca atgcgataaa tgatatatta gatgaccatt
     1201 cagtgctaga aaaagtttcg ggattatcta aaaagaaaca gaaacaaatt gcagaacaaa
     1261 tttcagcaaa tcaagaatct gaaaaaataa tgattcgttt acatgattta gggtttggtc
     1321 ctaaactttc aatggcaatt tatcagtttt atctcggcga tacactgact attttagatc
     1381 gaaatccata tcaattgatt tatgatatta aaggtattgg ttttaataaa gcagatcaac
     1441 ttgctagaaa tattggtatc gcttataacg ataatgaacg attaaaagct gcgttgctct
     1501 atacgttaga agaagaatgt attaaacagg gacatacata tttgcctatc aatgttgtta
     1561 tagatttaac ggttgatgta ttaaattatc aagatgaaga ggtcatcgaa cctgaaaaat
     1621 tagacgaaat gttgcaatac ttaaatgagg aaaaacgact aattattgac aatgaacaag
     1681 ttgcaattcc aagtttgtat tattctgaaa tcaaaagtgt tcaaaactta ttcagaataa
     1741 agacgcatac aaataaactt acagaaattg aacagtcaga tttacaaatg catattggtg
     1801 aaatagaaga tgcgaatcaa gtgaactatg cagcttcaca aaaagaagct ttgcaaacgg
     1861 ctatcaattc taaggtgatg ctattaaccg gtggaccggg aacaggaaaa acgacagtaa
     1921 ttaaaggtat tgttgaatta tatgctgaga ttcatggctt atcattagat tatgatgatt
     1981 atgtcaatga tgattatcca gttgtattag cagcgccaac aggaagagcg tctaagagac
     2041 tacaagagtc aactggatta gaagcaatga cgattcatcg tttaataggt tggaatcaag
     2101 acacaaagcc agaggatata ttagaaaatg aaataaacgc acgactcata atcattgatg
     2161 agatgtctat ggtagatact tggttgttcc accaattttt aagtgcagtt cctttagatg
     2221 cacaattgat atttgtaggt gatgaggacc aattgccttc tgtaggtcct ggtcaagtat
     2281 ttaaagattt aattgaatcg aaagctatac cacgagtaaa tctgactgaa gtatatcgtc
     2341 aacaagatgg ttcaagcatt atagaattag cacatcgaat gaagttgggt cagaaaattg
     2401 atattacaca gcgttttcat gatagaagtt ttattaactg tcaggcaaac caaataccaa
     2461 cagttgttga gaaggtagtt actagtgctg ttaataaagg gtatactatg gctgatattc
     2521 aagtgcttgc accaatgtat aaaggaaatg ccggtattaa acgtttaaat caagttttac
     2581 aagatatttt aaaccctaaa aagaaagaca cacgagaaat tgagtttggt gatgtagtct
     2641 ttagaaaagg tgacaaagta ttacaacttg ttaataggcc aaatgataac atattcaatg
     2701 gtgacatagg agttattgtt ggtatttttt gggcgaaaga aaatgccctt aataaagatg
     2761 tacttgttgt ggattttgaa ggtaatgaaa ttacatttac gaaacaagat atgatggaat
     2821 taacacatgc ttattgtaca tcaatccata aatcacaagg ttcagaattt cctattgtta
     2881 tcatgcctat tgttaaacag tattttagaa tgttacaaag accgatatta tatactggtt
     2941 taactagagc gaaaacgagt ttagttttac ttggtgatcc ggaagcattt gatattggat
     3001 tgaaaacaaa tggtcaagcg cgtttaacac aattatgtac attgttgaaa aattatttta
     3061 attcagaaga tgaagaaatg ctggaaaaca cagcaactaa tgatacagga gcttcacaaa
     3121 cgacaataga tgatcaagta gaagcgccaa tgtcatcgaa tgatagtgaa gaagttatgt
     3181 ctgattcaac aaaaacagat acaaatgtat taacggaagc tacaatgttt aaaattgacc
     3241 caatgattaa tatgggagaa atcacgccgt atgactttat agaacgttga caacaagcgt
     3301 aaatgtaact aaaatagaat taaattcgta taaagatgag tagttaatga tttgtttatc
     3361 agagatgtac tggttggtga aaagtacaac aaacattagt gaacgctact ttatgcacaa
     3421 ctgtatagtc gaattaaagt gataaatgtg ttgaattgca tttataacta gggtggtacc
     3481 gcgataacgt tcgtcccttt tgaggatggg cgttttttat tttttaataa taaaaaatga
     3541 tattgattta tactagtttt gcaaatcgac tatctgtgtc atcaattttt atggaatggt
     3601 aatttaaaaa cagtaaatag gatgtgtaaa tgatgaaaaa gttaaaagcg agtgaaatta
     3661 gacaaaaata tctagatttc tttgttgaaa aaggacatat ggttgaacct tctgcaccat
     3721 tagtgccaat tgatgatgat acattattat ggattaattc aggtgtagca acattaaaga
     3781 aatattttga tggacgtgaa acacctaaaa agccaagaat tgtaaactct caaaaagcta
     3841 ttcgtacaaa tgatattgaa aatgttggtt tcacagcgcg tcaccataca ttctttgaaa
     3901 tgttaggtaa cttctcaatt ggtgattatt ttaaacaaga agcgattgaa tttgcttggg
     3961 aatttttaac gagtgataaa tggatgggta tggagccaga taaattgtac gttacgattc
     4021 atccggaaga tatggaagca tacaacattt ggcataaaga tattgggctt gaagaaagtc
     4081 gtattattcg cattgaaggt aacttctggg atattggtga agggccttca ggaccgaaca
     4141 ctgagatttt ctatgatcgc ggagaagcat atggacaaga cgatccggca gaagaaatgt
     4201 atccaggtgg agaaaatgaa cgctatcttg aagtatggaa cttagtattt agtgaattca
     4261 atcataataa agatcatagt tacacaccat tacctaataa aaatattgat actggcatgg
     4321 ggcttgagcg tatggcctca gtttctcaaa atgtacgtac taactatgaa acagatttat
     4381 ttatgcctat aatgaatgaa atcgaaaaag tatcaggtaa acaatattta gtaaacaacg
     4441 aacaagatgt ggcatttaaa gtaattgctg accacattcg tacgattgca tttgcaattt
     4501 ctgatggtgc attacctgcc aatgaaggta gagggtatgt attacgtcga ttgttacgtc
     4561 gtgccgttcg ttttagtcaa acgttaggaa tcaatgagcc atttatgtac aaacttgttg
     4621 atattgttgc agacattatg gaaccatatt atccaaatgt taaggaaaaa gcagatttca
     4681 ttaagcgtgt tataaagtct gaagaagaac gattccatga aacattagaa gatggtttag
     4741 cgattttaaa tgaattaatt aaaaaagcta aagcgacaac aaatgaaatt aatgggaaag
     4801 atgcatttaa attgtatgat acgtatgggt tcccaattga attaactgaa gaaatagcag
     4861 tgcaagcagg attgaaagtt gatatgacaa cattcgagtc agaaatgcaa caacaacgtg
     4921 atcgtgcacg tcaagcacgt caaaattctc aatcaatgca agttcaaagt gaagtattga
     4981 aaaatattac atctgcaagt acttttgttg gttatgatac tgcgacagct caaacaacac
     5041 taacacactt gatatacaat ggtgaagaag tttcacaagt tgaagcgggt gaaacagtat
     5101 acttcatgtt aacggaaaca ccattttatg caatcagtgg tggacaagtt gcggatacag
     5161 gtattgttta taatgacaat tttgaaattg ctgttagtga agtaaccaaa gcaccaaatg
     5221 gtcaaaactt gcataaagga gtagtacaat ttggccaagt aaatgttggc gctacagtgt
     5281 ctgctgaagt gaaccaaaat gatcgacgtg acattcaaaa gaaccatagt gcaacacatt
     5341 tattacatgc agcgttgaaa tcagtactgg gtgatcatgt taaccaagct ggttcactag
     5401 tagaagcaga tcgtttacgt tttgatttct ctcattttgg tccaatgact aatgatgaaa
     5461 ttgatcaagt tgaacgctta gtaaatgaag aaatttggaa aggtattgac gttaacattc
     5521 aagaaatgga tattgcttca gctaaagaaa tgggcgcaat ggcattattc ggtgaaaaat
     5581 atggtgatgt tgtgcgtgta gtaaatatgg caccattttc aattgaatta tgtggtggta
     5641 ttcatgtccg caatacttct gaaattggct tattcaaaat agtaagtgag tcaggtacag
     5701 gagctggtgt gcgtcgtatt gaagcattaa caggtaaagc agctttctta tatttagaag
     5761 atattcaaga gaaatttaat acgatgaaat cacagctgaa agtgaaatct gatgatcaag
     5821 tagtcgataa gttaacacaa ttacaagatg aagaaaaagc attattaaaa caattagagc
     5881 aacgtgacaa agaaatcaca tcacttaaaa tgggtaatat tgaagatcaa gttgaagaaa
     5941 tcaatggcta taaagtattg gttactgaag tggatgtacc aaatgcgaaa gcaattcgct
     6001 cgacaatgga cgattttaaa tctaaactac aagatacaat tatcattctt gcaagtaatg
     6061 ttgatgataa agtatcgatg gttgcaactg tccctaaatc tttaacaaat aacgttaaag
     6121 ccggtgatct tatcaaacaa atggcaccaa tcgttggtgg taaaggtggc ggtcgtccag
     6181 atatggctca aggtggcggt acacaacctg aaaatatctc aaaatcatta agctttatta
     6241 aagattacat taaaaatcta taatttaaag ccataatatt gtatgatgat agtgagttat
     6301 atttgtaatt aaaggagtgt cgcaaatgga aaactttgat aaaacaatga aattcgacta
     6361 tgaagaactt ccaacgcaag atgtaagaga tgttttaaat aatgtttatc gaacattaga
     6421 tgaacgagga tataatgccg taaaccaaat tgtaggttat ttattatcag gtgaccctgc
     6481 gtatattcca cgccaaaatg aagcaagaaa tcaaattcgt catattgata gagatgttat
     6541 tatggaagag cttgtttctt actatttaaa agagcaaaat aaataattta tgttacaaca
     6601 taaaatttta ggactcgatg tcggtagtag aacggtagga attgcaatta gtgatataat
     6661 gggttggacg gcacaaggat tagacacact ccgaatcaat gaagaaaata atgaattagg
     6721 tattgaccaa ttagtagaca ttattaaaaa acataatgtg ggtacagtcg taataggact
     6781 acctaaaaac atgaataatt caataggatt tcgaggcgaa gcttcgttaa catataaaga
     6841 aaaattatta gaagcttatc cttctattga gattgtgatg tgggatgaaa gattaagcac
     6901 aatggctgct gaacgatcat tattagaagc agatgtttca agacaaaaaa gaaaacaagt
     6961 gattgataaa atggcagcgg tatttatttt acaaggctat ttagattcac tacattaaaa
     7021 ggagatttta ctatgactga acataatcat gattcacaac tagaaattaa taacgaagaa
     7081 gaattattaa ctttattcga tgaagaggga aatgaagttt tataccgaaa agttttagaa
     7141 ttttatcatc ctgaattcaa aaaagagtat gttatcttag ctgaagaagg tgctcaatca
     7201 gatgaagacg atatgattga gcttgtacca atgatcaatg aaccagatga gtcaggtgac
     7261 ggtggtaagt tagtaccaat cgaaactgat gaagaatggg acatgattga agaagttgta
     7321 aatactgaaa tggaagaata attaaaaaga ttttactctt gctaacctta aagatgggga
     7381 ggcaagagta ttttttaagt caagcaatgt ataaataatt aacgcttata attttattga
     7441 aaatatggta aaatatctaa agttaaattt tatatgaatg attcgcttct ataaataaaa
     7501 tagctttgcg tctaacacga aatcagtcat ttaattggtt ggcaaaatta taatcaattg
     7561 tgaaggctga tttattttat tgtcataatc atattgactt attttacata aaggaagttt
     7621 aaaacatgga tgacctaaat aaaaaatatt taatagattt acatcaacat caaaatagtt
     7681 caatcgaagt tttgcgtgaa tttgccgagg taaatgaagt gccaattgta gatcgtttaa
     7741 cattagattt aattaagcaa ttaattcgta tgaataatgt taaaaatatt ttagaaattg
     7801 gtacagcaat cggctatagt tctatgcaat tcgcttctat atctgatgac attcatgtca
     7861 caacgataga gcgtaatgaa acgatgattc aatatgctaa acaaaattta gctacttatc
     7921 attttgaaaa ccaggttcga attattgaag gtaatgcttt agagcaattt gaaaatgtaa
     7981 atgacaaagt ttatgatatg atatttattg atgcagcaaa agcgcaatca aagaaatttt
     8041 ttgaaatata tacaccactt ttaaagcacc aaggtctcgt aattacagat aatgttttat
     8101 atcacggttt tgtatcggat attgggattg ttagatcgag aaatgtaaga caaatggtta
     8161 aaaaggttca agattataat gagtggttaa taaagcaacc aggatataca acgaattttt
     8221 taaatataga cgatggatta gcgatttcaa ttaaaggaga atgactatga cagaattact
     8281 tgtcacaccc aagtcattaa gccatatgga aacacttatt gatttaggtg cagatgcttt
     8341 tgtaattggc gagcaaaagt ttggtttaag acttccaggg gaattcaata gacaacaaat
     8401 gacagaggct gtcgcattag ctcacaaaaa cgataaaaaa gtatatgcgg cagtgaatgg
     8461 attatttcat aattatcatt tagatgctgt agaagattat attaattttt tacatgaaat
     8521 acgagttgac agaattattt tcggggatcc agcagtagta atgtatgtga aggcgcaaga
     8581 aaatccaata ccacttcatt ggaatgcaga gacgttagta actaatcatt ttcaatgtaa
     8641 ttactggggt aaaagaggcg catcaagagc tgtcttagca cgtgagctga atttggatga
     8701 aattattaat ataaaagaaa attcaaatgt ggaaattgag gtgcaagttc aaggaatgac
     8761 ttgtatgttt caatcaaagc gtatgctact aggcaattat tatacgttcc aagatcgcca
     8821 aatgaaaatt gaacgtcgca atgatgaaca atctttatta ctttatgatg aagaaagaca
     8881 aaataattac ccagtttacg aagattacaa tggtacgcac attatgtcac caaacgacat
     8941 ttgtttaatt gaagaattag caccattttt tgaggcgggt atagattcat ttaaaatcga
     9001 tggtattcta caaacggaag aatatattaa tgtggtaacg gaacagtatc gtcaagcgat
     9061 agatttgtac aatgaagatc ctgaaatcta tgaagatgag aaatttatgt tgatggatcc
     9121 aattgaagaa attcaacctg atcatcgacc atttgacgaa ggtttcttat ataaacaaac
     9181 agtatattaa ggaggttaat catgaagaca atagaagaga ttaaatcaac tcctaaaaca
     9241 gttatgaaga aaccagaatt attagcacct gctggaaact tagaaaagtt aaaaatagca
     9301 gtacattatg gcgctgatgc cgtattttta ggtggtcaag aatatggatt acgttcaaat
     9361 gctgataatt tcacgatgga agaaatagct gaaggtgttg aatttgcgaa ccgttacggt
     9421 gccaaaattt atgttacgac aaatattatt gcacatgatg agaatattga aggtctagaa
     9481 tcatatttgc gtaatttgga aaagactggt gcgacaggta tcattgttgc agatccttta
     9541 attatcgaaa catgtaaaga agttgcgcca aaacttgaaa ttcatttatc tactcaacaa
     9601 tcactttcta attacaaagc tgtagaatat tggaaagaag aaggattgga tcgtgttgta
     9661 ttagcacgtg agaccggcgc gatggaaatg cgtgaaatga aggaaaaagt agatattgaa
     9721 atcgaagcat ttattcatgg tgctatgtgt atcgcctatt caggtagatg tacattaagt
     9781 aatcatatga ctgcaaggga ttccaacaga ggcggttgct gtcaaagttg ccgttgggat
     9841 tatgaattat tagaagttga tgataatggt gaacttgatg ttttttataa tcaaggtgaa
     9901 gttacaccgt ttgcgatgag tcctaaagat ttaaaattaa tcgaatcaat tcctcaaatg
     9961 atggatattg gtgtggactc attaaaaatt gaaggacgta tgaagtcaat tcattatatt
    10021 gcaacagttg tctcagtata tcgtaaagtc attgatgcgt atgcggcaga tcctgacaac
    10081 tttaagatta atccggaatg gttaatagag ttagataaat gtgcaaatag agacactgca
    10141 ccagcattct ttgagggaac acctggttat gaagaacaga tgtttggtca acaacaatct
    10201 aaaaaatcac catttgattt ttgtggtttg gtattagact ataatgaaga tacaaaaatt
    10261 gcgactattc aacaacgaaa taactttaaa ccaggtcaag aaatagaatt ctttggtcca
    10321 gaaattgaaa catttacaca agtagtagaa gcaatttatg atgaagaagg taacagctta
    10381 gacgcggcgc gccatccatt acaaatcgtt caaattaaag tggatcgccc gatatatcca
    10441 aacaacatga tgagaaagga aattggctaa tgaaagctac tacaatcatt ggcatagctg
    10501 gtggatctgg ctcaggaaaa acaactgtaa ctaacgaaat tatgaaaaac ttagaaggtc
    10561 atagtgtcgc tttacttgct caagattact attataaaga tcaaaagcac ttgactttcg
    10621 acgagcgcct agaaaccaat tatgaccatc catttgcatt cgataatgat ttattaattg
    10681 aaaatcttaa agacttgaaa aatggtaaag cagtagaagt accgacatat gattatgcta
    10741 gtcatacaag aagtgacatt accattgatt ttaaacctaa agatgttatt atcgtagaag
    10801 gcattttcgc tttagaaaat aaggtattac gtgatatgat ggatgttaaa atatatgttg
    10861 atacagatgc agacttgaga atattacgcc gtttaacacg agatactaaa gagcgtgggc
    10921 gttcaatgga ctctgttatc aatcaatatt taagtgttgt tagacctatg catgaccaat
    10981 ttattgaacc gactaagaaa tatgctgata taattattcc tgaaggtggg agcaataaag
    11041 ttgcaataga tattatgaca acaaaaattc agtctttagt tagcaagcaa tagtataatt
    11101 cagtaaagga agatgacaat atggaaaatc aaaagcaata tccaatgact caagaaggtt
    11161 ttgaaaaatt agagcgtgaa cttgaagaat taaaaacagt taagcgtcct gaagttgtag
    11221 agaaaattaa agttgcacgt tcatttggtg acttatcaga gaactctgag tatgatgcag
    11281 caaaagatga acaaggattc atcgaacaag atattcaaag aattgagcat atgttaagaa
    11341 atgcattaat cattgaagat actggagata acaacgttgt taaaattggt aaaacagtaa
    11401 cgtttgtaga attaccaggt gatgaagagg aaagttatca aatcgttggt tcagctgaat
    11461 cagatgcatt taatggtaag atttcaaatg aatcaccaat ggctaaagcg ttaattggta
    11521 aaggtttaga tgatgaagtt cgtgttccac tacctaatgg tggcgaaatg aacgtaaaaa
    11581 ttgttaatat ccaataaatg atcaaataga ttatttaatt gcgcgattga agtgtgttaa
    11641 tgatacttcg gtcgtgcttt tttatattca aaatcaaaaa atcggacaga tgaaaagtaa
    11701 aaattttcca tcagaccgaa tgatagcaga acctaaatta gtgtaagagt tacaatatta
    11761 actactccat tttaaaaaag caaaatattt ccgagaaaac aaattgatta tcatttgtta
    11821 ttgtatgcga tttgaattac ttattaaaca acttgtgctt ctcaatgata caatgaaaag
    11881 gtattcaata ctggtttatt gcaatacaaa gggggattta aagtggatgt aagatttatt
    11941 aacgagcaga caattatgat ttattttgaa aataaaatat ccgaagagac atatcgtaat
    12001 gtcacagcaa tggtacggtg gatacgagaa aaagaaatat tagaaattca agatattgtc
    12061 ccttcgtatc gagcagttct aatatatttt gatgaacaag ctataacatc ttctaaatta
    12121 attgaaaatt tagaacttaa caaatttaat gaaaaaaatg ttcatgctgt gaatcaaacc
    12181 aatcgtatta ttaaaatacc tgttcaatat ggtggtacat atggaccaga tattgaagaa
    12241 gtagcaaagc ataatcgaat aactgttgag caagttattg aaaaacatac aagtaaacct
    12301 tatttaatat atatgctagg atttatgcca ggatttccat acttaggcgg acttgatgaa
    12361 caactacata cacctagacg taatcagcca aggttaaaaa ttcatgcagg ttctgtaggt
    12421 attgcgaata atcaaacagg actatatcca tcagattctc ctggcggatg gcaaattatt
    12481 ggacgcacac cgttaaaagt atttagcagc gagcgtgaac caatgtcaat gtacgaagca
    12541 ggagaatgga tacaatttta tgcaatagat gaacagaaat ttatacaaat cgaaagagat
    12601 atatcagatg gcaactttaa tgttgatgac tgggtggtga ttgaaaatgt caattaagat
    12661 tttacaacca ggtctctttt caacggtaca agatctagga agaataggtt atgaacatat
    12721 tggattttca ggcgcaggtg ccatggatca atttagtttt aaggttgcgc agtcattaat
    12781 taacaatgat ggtccagcga ttgaatatac tttgattggt cctaccattc aatttaatac
    12841 gcaaaataca tttgttataa ccggtggtag tattaatgct tcgctaaata ataaaactat
    12901 atcaatgaat tctgtcatat tagctgagaa aggtgacatt ttaaaaatag gtgctataac
    12961 caaaggtgca cgcggttatc ttacttttgg tcattctatc aacgtacctt ctattgctga
    13021 aagttatgca acacataccc gaagcagtat aggaggattc aaaggtagaa agttattagc
    13081 tgatgatgta ataacagtga aaataaataa tgactttaaa gaaaacattg gaaagacgat
    13141 tcatttacag gacgatttat tgccagaaaa taatattata cacattcttc aaggacctca
    13201 attcgaggca ttttctgaag aggctagagc gaaaattgta aatcatccat atttaattac
    13261 tgaacaatca gaccgtatgg gctatcgttt ggaaggtgac agcgttgcac caattaatca
    13321 agcagatatc atttctgaac cggttgctct tggtagtgtt caggtaccaa atgatggtca
    13381 acctattatt ctgcttaatg ataaacaaac gataggtggt tataccaaaa ttgcaactgt
    13441 atgcaaattt gatctgccaa agttagcaca gatgaaacca caagatacaa ttcaatttaa
    13501 atggatatca ttccaagaag ctgtagataa gaatcgggaa caaatgtctc tgtttaatga
    13561 aattcttaag agtcatcaaa agactccaat ttttgataca tctagcttgc gacatacttc
    13621 gaaaaaatta gcaacaattt taaaagggga tttgtaaaat gaatattgaa aaaatcgaac
    13681 aaataatcaa attagtgaag gaaaatgatg ttaagaaatt taaatataaa aattttgaag
    13741 atgaaataga aattgacttc actgactcga atcatttggc tgcacacagt aatcaatcaa
    13801 atcaaagtat gaacaataat gatttgacag cttcaaaagc gaatgataac tccgatgttt
    13861 cgacaaatga ttatcatgac attaaatcac caatgatagg tacattcttt ttacaagata
    13921 gtaaagaatt aactgaacca attgtgaatg tcggtgacaa agttaacaag ggagatatta
    13981 taggatatgt tgaagcgatg aaagtattaa acgaggtaac aacagatgtt gctggagaaa
    14041 ttactgaaat agtagctgat catggcacaa atgttgaata cgaccaagtt ttggtacgta
    14101 ttaagtaaag ggggtaagta aatgcttcgt tgtttaattg cgaacagagg tgaaattgct
    14161 gtaaggatta taagagcttg cagagaatat gggattgaaa ctgtagcagt ttatgcaaaa
    14221 ggggacgagc aaagcttgca tgtacattta gcagatcaag ctatatgtat tggggaagct
    14281 aatgctttag atagttattt aaatattgac cgcatcatat ctgctgcaca aatcactggt
    14341 gctaatgcaa ttcacccagg atatggcttt ttatcagaat caacaaaatt tgctcaaacc
    14401 gttgaggaac aaggcatagc gtttataggg cctactaaaa aaacgatgga aatgatgggg
    14461 gataaaataa cagcaagaca aacagttcat catgcaggtg ttcctgtgat tccaggatca
    14521 aatggtgctg tgaatcatgt gtctgaaatt gagaatcttg ccaaagacat cggttatcca
    14581 gttgttatca aagctgccag tggcggtggt ggtaaaggta tacgtattgt aaagaaagct
    14641 gaagatttag aaaaggcatt taaagaagct aaaagtgaag gaaaaaaata ctttgatgat
    14701 gatcgtgttt atgttgaggc ttttatacct gttgcaaaac atgttgaagt tcaagttatg
    14761 ggagatgggc aggataatta tgtacattta ggggaacgtg attgttctgt acaacgtaaa
    14821 aatcaaaaat taatcgaaga atctccttgt gcagcattaa ctgaagaaag acgacaacaa
    14881 atatgtaacg atgctgttaa agttgcacgt gcagcaaatt atagaagtgc aggtacgatt
    14941 gaatttttag taactgatac cgcacattac tttattgaaa tgaatgctcg aatacaagtt
    15001 gaacatactg ttacagagat gcgagcagaa cgtgatttgg ttgcagcaca actgtattta
    15061 ttggaacata atcatttacc tttttcacaa agcgatattc aatttaatgg tcatgtgata
    15121 gaagcgagaa taaatgccga aaatcctgaa aagaaatttc aaccaacacc aggtaaagtg
    15181 acagcgttac atttacctca aggctttaat gtgcgagtcg attctttact atatcacggt
    15241 tatcaagttt caccatatta tgattcttta gtagctaaag taattgtaaa atctcatgat
    15301 agagcttcag caattgataa gttgaaagtc accttagatg aaatggtgat agatggattt
    15361 tctacaactg ccgatttttt atatgcagtt ttaaattatc cactatatcg tgatggagat
    15421 gcaaaagatg ttgatattaa atttttagaa aaacatcaaa ttgttaaagg ggtggaatta
    15481 taatgcgagt tgatttgaat tgtgatttag gcgaagcatt tggaaattat tcctttggtg
    15541 gtgatcatca aattattccg ttaattacaa gtgcgaatgt tgcttgtggt tttcacgctg
    15601 gtgatgaaaa tgtaatgaat gaaacggtaa aacttgccaa agcacataat gttgcagtag
    15661 gtgcacatcc tggtttacct gatttgaaag gctttggcag acgaaatata gatatctcta
    15721 acgacgagat ttataatttg atgatttatc aattaggtgc attacaaggg ttttgtcgca
    15781 ttcatcaagt taaaattaat catgttaaac cgcatggtgc attgtatcag atgggtgcaa
    15841 aagacagaga aatagcaaac gttatagcac aagctgttta tgactttgat ccatcactag
    15901 tgttagtagg attagcaaat tcatatctaa tttcagaagc aaagaatgtc ggattaatta
    15961 cagcttctga agtgtttgct gatagacgat acgaagatga tgggcagctc gttagtagaa
    16021 aagaaagtga tgctgtgatt actgatactg acgaagcact taagcaggtt ttaaagatgg
    16081 tgaaggaaaa taaagttatt tcaaaaaaca ataaggaagt aacgttacaa gcagatacaa
    16141 tttgtgtgca tggtgatgga gaacatgcat tattatttgt ttcgaaaatt agagaaattt
    16201 taatgaaaga aggcattgat attcaatcct tatagggggc taaaaaaatg gggagaaatt
    16261 taaagcttaa aaaagaaagt gactttgaat tcacaaaaaa tcataaaagg ttattattag
    16321 gttctgtatt tttgatggca acttctgcaa ttgggccagc atttttaacg caaacagcag
    16381 tatttacatc acaatttttt gcaagtttcg catttgccat attactgtct atcatcattg
    16441 acattggtgc acaaattaat atatggcgca tattagttgt aactggttta agaggtcaag
    16501 aaatatcaaa taaagttgtt cctgggcttg gtactgttat ctcaatattg attgcatttg
    16561 gtggtctagc ttttaatatt ggtaatattg ctggtgccgg tttaggttta aatgcaattt
    16621 ttggattaga tgtaaaatgg ggcgcagcta ttactgcaat ctttgcaata ttaatctttg
    16681 taagtaaaag tggccaaaaa attatggacg ttgtttcaat gattcttggt attgtgatga
    16741 ttttagttgt ggcatatgtg atgtttgttt ctaatccacc ttatggtgat gcttttgtgc
    16801 atacatttgc gccagaacat ccaatgaaat tagtcttgcc catcattacg ttagttggtg
    16861 gaactgtagg tggttatatt acctttgcag gtgcacatcg tatattagac tctggcatta
    16921 aaggtaagca atatttacca tttgtaaatc aatcagcaat tgctggtatt ttaactacag
    16981 gtattatgag aacgttacta ttcctagcgg tattaggagt tgttgtaaca ggtgtgacac
    17041 taagttctga aaatccacca gcgtcagttt ttgaacacgc aattggacca attggaaaga
    17101 atatttttgg tattgtgtta tttgctgcag ctatgtcatc agtaattggc tcagcataca
    17161 caagcgcaac atttttaaaa acacttcata aatcacttaa cgaaagaagt aatttaattg
    17221 tgattgtgtt tatcgttatt tcaacaatga ttttcttatt tattggaaaa ccaatcagcc
    17281 ttttaattat agcaggcgcg ataaatggat ggattttacc tattacgtta ggtgcaattt
    17341 tgattgcaag taaaaagaaa tcaattgttg gtgattataa acacccaaat tggatgttca
    17401 tctttggtat cgttgcagta cttgttacaa tattgacagg tatattctca ttcaaagaag
    17461 tacttcagtt attttaaaag acatttcgac taatagcatc actgtaaatg ttgttagtat
    17521 taggcaagat atattttgat ttccggtgat tcatttataa ag
//
LOCUS       DAKTGS010000049        17182 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.049, whole genome shotgun sequence.
ACCESSION   DAKTGS010000049 DAKTGS010000000
VERSION     DAKTGS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.049"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(85..318)
                     /gene="nrdH"
                     /locus_tag="O0286_001350"
     CDS             complement(85..318)
                     /gene="nrdH"
                     /locus_tag="O0286_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCY0169734.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            516..1877
                     /locus_tag="O0286_001351"
     CDS             516..1877
                     /locus_tag="O0286_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCY0169735.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1874..2893
                     /locus_tag="O0286_001352"
     CDS             1874..2893
                     /locus_tag="O0286_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCY0169736.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            3026..3688
                     /locus_tag="O0286_001353"
     CDS             3026..3688
                     /locus_tag="O0286_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCY0169737.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(4037..5734)
                     /locus_tag="O0286_001354"
     CDS             complement(4037..5734)
                     /locus_tag="O0286_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0169738.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(5734..5952)
                     /locus_tag="O0286_001355"
     CDS             complement(5734..5952)
                     /locus_tag="O0286_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCY0169739.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(5981..6103)
                     /locus_tag="O0286_001356"
     CDS             complement(5981..6103)
                     /locus_tag="O0286_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169740.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(6433..6984)
                     /locus_tag="O0286_001357"
     CDS             complement(6433..6984)
                     /locus_tag="O0286_001357"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0169741.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            7349..7975
                     /locus_tag="O0286_001358"
     CDS             7349..7975
                     /locus_tag="O0286_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCY0169742.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            8146..9258
                     /gene="pdhA"
                     /locus_tag="O0286_001359"
     CDS             8146..9258
                     /gene="pdhA"
                     /locus_tag="O0286_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCY0169743.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            9262..10239
                     /locus_tag="O0286_001360"
     CDS             9262..10239
                     /locus_tag="O0286_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0169744.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            10330..11622
                     /locus_tag="O0286_001361"
     CDS             10330..11622
                     /locus_tag="O0286_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0169745.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            11626..13032
                     /gene="lpdA"
                     /locus_tag="O0286_001362"
     CDS             11626..13032
                     /gene="lpdA"
                     /locus_tag="O0286_001362"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0169746.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            13200..13475
                     /locus_tag="O0286_001363"
     CDS             13200..13475
                     /locus_tag="O0286_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCY0169747.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            13619..14158
                     /locus_tag="O0286_001364"
     CDS             13619..14158
                     /locus_tag="O0286_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0169748.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            14171..15265
                     /locus_tag="O0286_001365"
     CDS             14171..15265
                     /locus_tag="O0286_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169749.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            15258..16055
                     /locus_tag="O0286_001366"
     CDS             15258..16055
                     /locus_tag="O0286_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169750.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            16061..16870
                     /locus_tag="O0286_001367"
     CDS             16061..16870
                     /locus_tag="O0286_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169751.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            16870..>17182
                     /locus_tag="O0286_001368"
     CDS             16870..>17182
                     /locus_tag="O0286_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0169752.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LL"
BASE COUNT         5900 a         2501 c         3140 g         5641 t
ORIGIN      
        1 agttctttaa ttaatgttag ttaatttaaa aatcgatatt taatttatga tagctataaa
       61 tgatgaagtt gatttagatg aaaattaatc ttggatattt aatactttgt tgatttcatc
      121 aagatcaaca tggtacattg gattgccatt caacaaaata aacggagttg aaaaagcatc
      181 aaaatctatc atttcgtttc gatattgttg attgttgata tttctctctt caaaatcaat
      241 gtgatgctca tttagataat tttttacaaa tgtacaaggt ggacaatcat tctgcgtata
      301 aacgattatt tctgacatgt atcatgctcc ttcgtgcttt attctaatgt aatatacaca
      361 attatacatt gaaattcaaa tttgtgaaca cattgtgaac tgacataaat tatacacaat
      421 ttcatagcgc ataatgtttt aaaaatacac attatagcaa ttataattaa gttcattgtg
      481 ttgtcatcaa aaaaagaaaa aggtgatgtt tttaaatgga tacagttgaa atcagtcggt
      541 ttttgacagc tatgacttta gcagttcata tcatttttgc aacgattggt gttggtatgc
      601 ctttaatgtt cgcaattgca gaatttttag gtattcgcaa aaatgatctt caatatatag
      661 ctatggccaa aagatgggct aaagcttata caattactgt agcagtggga gttgttacag
      721 gtacaattat aggacttcaa ttatcattga tttggcctac atttatggaa atgggtggac
      781 acgttattgc acttccatta tttatggaaa catttgcgtt cttctttgaa gctattttct
      841 taagtatata tttatatact tgggatcgtt ttaaaaataa atggacacat ttcttaatta
      901 gtataccagt aattattggt ggctctttct cagcattctt cattacttca gtgaattcat
      961 ttatgaatac gcctgcaggt tttgagttga agaatggaaa gatggtcaat gttcaaccta
     1021 tagaagcgat gtttaaccca tcgtttatag ttcgatcatt tcacgtaatt actacagccg
     1081 gtatgacgat ggcatttgtt attgcatcaa tagcagcttt taaattattg cgtaatcgtc
     1141 aacctaaaga tactgtctac cataagaaag ctttgaaaat gtctatgata gttggattct
     1201 tttcaacatt actttctatg ttggcagggg atttatctgc aaaatttttg cataaattcc
     1261 aacctgaaaa attagcagct tatgaatggc atttcgatac atcttcccat gccaaattat
     1321 tattatttgg tgtgttagat gaaaagactc agcaagttaa aggtgcgatt gaattacctg
     1381 gactattgag tttcttagca gataatagtg tcaaaactaa agtacaaggg ttaaatgatt
     1441 ttccaaaaag tttacatccg cctatgatcg tccattattt ctttgattta atggtaacga
     1501 tgggaatttt atgttttgtc atttcaggtg tttatgtctt aactttaatg tttaaaaagc
     1561 ttagaaagtt ttctactcat aaatggatgc tttatggaat attattaaca ggcccagctt
     1621 caatgctagc tatagaattt ggatggttct taacagagat gggtagacag ccttggattg
     1681 ttcgtggtta tatgcgcgtg gcagaagcag caacacaagc aggcggaata accttcgtta
     1741 caattttatt tggcatattg tacatcattt taatgtatac atgtgcatac gtattaattc
     1801 gtatgtttaa aaataaaccg gcgtatgaag atgtaaatcg tttagccaag aagcaaggag
     1861 gagaaataga aaaatgattt atgcatttat aggtataaca gtgttatggt tgtttttatt
     1921 ttgctatatc attattgctt ctatagattt tggggcaggt ttcttcgcat tgcattcaaa
     1981 gttaactggt gatgaaaaga aaattaatca cttaatttca cgttatttaa acccagtttg
     2041 ggaagttacg aatgtattct ttgttttctt cttcgtagga ttcgtaggtt tctttccaga
     2101 gtcaatcaaa tatttaggca cggtattgtt aataccaggt tcaatagcac tgattatgat
     2161 atcgttgaga aacagttttt atgcatttga aaattatggt caagatacaa aattagcatg
     2221 gatgatcatg tatggggtaa gtggattatt aattccagct tcattatcta ctgctttaac
     2281 tattacagaa ggtggctata ttaatgttcg aaacaatgtt atcgatctag attgggtgca
     2341 gttattatta agcccatttg cttggtctgt agtattcttg gcaattattt cagttttata
     2401 tatttcatca ggatttttga catattatgc taaaaaagca aatgacgaac cagcatataa
     2461 tttaacaaga caatggcaca tatttttagg gccgccgatg attatcattt gtttattcgt
     2521 atttctatca ttacgcattc aaaattctga acatttttat tcagctgttt ttgactattg
     2581 gtggatgttt ggtataagtt tcttattctt tgcattagct tcattgttaa cattctttaa
     2641 gaaaaaacat ggtttggctt ttgtatttgt tattttacaa atgatgttcg cgttctttgg
     2701 ctatggaatt agtaaattgc catacctttt atatccgttt gtaaaaatta cagatgcata
     2761 tgttaatcca gaaatgggct ggacattagt gattgtcttt attttaggtt tacttttatt
     2821 acttccatcg ttaatattat tattaagatt atttgttttc gacaaagaat atgttgaagg
     2881 aaagaaatca taagttgaga cgatacccca actttcattt acttgcatta ctgcatttag
     2941 ataatttagt tggggtactc atttataata attttaaatt tatattttca atgaattgat
     3001 gaaaatcatt taatggaggg cgagtatggg taaagaatat gtagtcatcg gtctaggtcg
     3061 ttttggaggt agtatcgttc gtgagttgaa tgcattagac atggatgtaa tggccatcga
     3121 ccatgatgaa aatagagtga atgaatatag tgatatcgct actcatgcgg ttgttgcaga
     3181 cactacagat gaagcagtta tgaaaagttt aggtatccgt aactttgatc atgtcattgt
     3241 ggcaattggt gaaaatattc aatcaagtac gttgacgact ttaattttaa aagagttagg
     3301 tgtaaaaaaa gtaactgcta aagcacaaaa tgattatcat gcaaagattt taaataaaat
     3361 tggagcagat acggttgtgc accctgagcg tgatatgggt agacgtattg cgcataatgt
     3421 tgcgagtgca agtgtacttg attatcttga gttggcagac gagcattcta ttgtagaatt
     3481 gaaagcaact gaaaagatgg cggggcagtc tatcattgat ttagatataa gagcacaata
     3541 tggaattaac attattgcaa ttaaacgagg caaagagttc atcatttcac caaatccaaa
     3601 tattaattta gaaataggtg atattttaat catgattgga catgataatg atttaaatcg
     3661 ctttgaaaaa aatattgcga cgagataatc gggagtggga cagaaatgat attttctcaa
     3721 aatttatttc gtcgtcccac cccaacttac attgtctgta gaaattggga atccaatttc
     3781 tctttgttgg ggcccacacc ccaactcgca ttgcctgtag aatttctttt cgaaattctc
     3841 tatgttgggg cccctgacta gaattgaaaa aagcttgtta caagcgcatt ttcgttcagt
     3901 caactactgc caatataact ttgtagagca ttgaacattg atttatgtct caagctcatc
     3961 atatataaag aaaaagctac taacaatggg tggtgagttt aattaaaact tcacttcaaa
     4021 gttagtagct ttttgattat ttattgtttg attctttttg ttcgtttacc ttcataatga
     4081 ctggtaaaat cattggtttt ctagctgttt tttcaaataa ataaggttgt aatgtttcaa
     4141 tgatagaaga tttaatctga tgccattgaa tatctttatt ttgatttaac ttactaataa
     4201 catcagtttt gattttgcgt tgtgcatcat aaattaattg acctgattcc ctcatatata
     4261 caaatcctcg agaaataatg tctggaccag aaagtaattt atttgtatta aaatcaatac
     4321 taacaacaac gataactaaa ccttcttcag ataatagctt acggtctctt attacaacat
     4381 taccgatatc accgatacca ctaccatcaa caagtacatt accagatgga atgcgaccag
     4441 ctttacgtgc tgaatcgtgt gttaaagcta agacatctcc aatatcaaag atgaagacat
     4501 tatcttcttc aacgccgcat tcaacaccag tctcaccatg tgcttttaac atacggtatt
     4561 caccatgaat aggtaagaaa tatttcggct tgattaatcg aagcattaat tgttgatcac
     4621 cttgagaacc atgccctgaa gtatggatgt tagaaatctt gctatggata acatctgcac
     4681 cagctttata caaggaatta atagttctgt taatactttt tgtattacct gggataggtg
     4741 atgaactaaa tacaacggta tcttcaggta taattttaat ttgcttatga gtaccattag
     4801 caattctaga taatgctgcc attggttcac cttgtgaacc agtacatagt atcaataact
     4861 catgcttcgg tacggtatta attttattag gttcaataaa tgtttcaggt ggtgctttaa
     4921 tataaccaag ttccatacct attttaatat tgttttccat cgaacgaccg aacgtaacaa
     4981 ttttacggtt atttttgata gcagcttcaa ctgcttgttg aactcggtaa atattagaag
     5041 cgaaggtagc aaatataata cgacctttac aattacggaa gatcttatct acgttttgac
     5101 caacttcacg ttcgcttaaa gtaaaatcag gcacaagtga atttgttgag tctgaaagta
     5161 aacatagaac gccttcttcg cctaattgag ccattttagc aatgtttgct ggtttgccta
     5221 caggtgtaaa atcaaattta aagtcaccgg tatgaactac ttttccttca ggtgtatcta
     5281 cgatgacgcc ataagtttca ggaatactat gtgtagttaa gtagaaagaa atcgtaaagt
     5341 gcttagattt aatcacactg tcctcattga tttcatttag tttagcagta cgtaataaat
     5401 gatgttcttc aagtttatta cggattaaac ctaatgctaa aggaccacca taaataggta
     5461 tattaagttg ttttaatagg aagggcacac cgcctatatg gtcttcgtga ccatgtgtta
     5521 taaataggcc aacaatttta tcttggtttt gaactagata tgtgtagtca ggtataacat
     5581 aatcaatccc taataagtta tcatcaggga atttgatacc tgcatcgata atgacaattt
     5641 cgtctttata ctcaactgca taagtatttt taccgatttc acctagacct ccaagtgcat
     5701 atacacctac ttcatttgga tgtaattgtt tcattattta gcaatctcca cattaaagtg
     5761 ttctgagttt tctttttcgt aatctaaatg tgcgccctct aatttagtga taaattcgat
     5821 attaaaatta cgatctttca agtaacgacg tacttgttct tctgtttgag cttcaacata
     5881 aagtgattgt gtattttcac gcacaattac ctcgtctctg ttatgttgat aaaaaacttt
     5941 aaatactgcc atgttaaaaa ttcctcctaa gaatgtttgt ttaatttatt gttaacccta
     6001 gtaaaatcgt attggagtat atatcgataa attcattcca atcatctcta taatgtaact
     6061 taataacgat ttggttaata actaggttca tcatgtcgtt cattttaaaa aattagtgaa
     6121 ataacactaa aatttcagtt aaatcttatt ttacatgatg aatgataata aataaagcag
     6181 tttatctcac tagaaatcta agtatttcga ataaatgtgc agttctattt attaaaataa
     6241 atgtagagat tataaattaa tgttaagaaa gaattaaagg attttatatg aaacggtaaa
     6301 atttaaatga ggggataaaa agtttggtag aaaggtgaat atatttaact gttgtttatt
     6361 cgaaaaagtt gtaaaaggaa aagcttagga catcatattt atcctaagct ttgataatta
     6421 gaaaaatgtg ttttaaactt ctactgcatc tgtatgtggt tgtaatgggt gatctttgtc
     6481 aatgtgatca tagaacatta caccatttaa atggtcaatt tcatgttgga aaacaattgc
     6541 tggatatcct tttagtcgta attgtatatc attaccttcg atgtctttgg ctttaattgt
     6601 aattctatta tgacggtgaa ctagaccagc aacattatca tcgacactaa ggcaaccttc
     6661 accagttggt aaataagctt cttgaacgct atgacttaca atttttgggt tcacaagcat
     6721 atagtcataa gatttgccac tgccatcatc tggtattaaa acagcaatca tacgtttaga
     6781 aatattaatt tgaggtgcag ccaaaccaac gcctgaacgt aaaccatatc gtttcgcgat
     6841 ttcctcatct tgactattta ctaaaaactc tctcatggcg attaatgttt ctttttcttc
     6901 tttagttaat ggtaattcta actcagctgc tttttgacgc aaagttggat gaccatctct
     6961 aatgatgtct ttcattgtta acatatattg caccttcctt attttaattt gttttagttg
     7021 aatgacagta aaaaggttgt taagatactc atacattttt atgtgtaaat atctacaaag
     7081 ttaaccaact actgccaatg tttattttag atagtatatg taaattttca gatatgctaa
     7141 ttgcttaaaa aatgattaaa gtgttggttt caagcaatga tactttagaa atttatttat
     7201 catcttgact ttaaaaatta tattataaat gacgtaactg tcaacagata tacttagtaa
     7261 tgaagatgtg taatgtaatt gtttaaaatt gatttccaag cagattttat ttatcattta
     7321 atttaaatag caagtggagg tacaagtaat gaaatttgga aaaacaatcg cagtagtatt
     7381 agcatctagt gtcttgcttg caggatgtac tacggataaa aaagaaatta aggcatattt
     7441 aaagcaagtg gataaaatta aagatgatga agaaccaatt aaaactgttg gtaagaaaat
     7501 tgctgaatta gatgagaaaa agaaaaaatt aactgaagat gtcaatagta aagatacagc
     7561 agttcgcggt aaagcagtaa aggatttaat taaaaatgcc gatgatcgtc taaaggaatt
     7621 tgaaaaagaa gaagacgcaa ttaagaagtc tgaacaagac tttaagaaag caaaaagtca
     7681 cgttgataac attgataatg atgttaaacg taaagaagta aaacaattag atgatgtatt
     7741 aaaagaaaaa tataagttac acagtgatta cgcgaaagca tataaaaagg ctgtaaactc
     7801 agagaaaaca ttatttaaat atttaaatca aaatgacgcg acacaacaag gtgttaacga
     7861 aaaatcaaaa gcaatagaac agaactataa aaagttaaaa gaagtatcag ataagtatac
     7921 aaaagtacta aataaggttg gtaaagaaaa gcaagacgtt gatcaattta aataattaat
     7981 ataatacaga tggtaggaaa caactaatac agttcctatt atctgtatct ttttttatta
     8041 aaacagaact ttttcaaatg gtttaacagt cccatttatt tgtggtacaa ttagtaagga
     8101 taaaatgaat ttctatacaa ttatgggaaa ggtatggtga attgaatggc tcctaagtta
     8161 caagcccaat tcgatgcagt aaaagtttta aatgatactc aatcgaaatt tgaaatggtt
     8221 caaattttgg atgagaatgg taacgtcgta aatgaagact tagtacctga tcttacggat
     8281 gaacaattag tggaattaat ggaaagaatg gtatggactc gtatccttga tcaacgttct
     8341 atctcattaa acagacaagg acgtttaggt ttctatgcac caactgctgg tcaagaagca
     8401 tcacaattag cgtcacaata cgctttagaa aaagaagatt acattttacc gggatacaga
     8461 gatgttcctc aaattatttg gcatggttta ccattaactg aagctttctt attctcaaga
     8521 ggtcacttca aaggaaatca attccctgaa ggcgttaatg cattaagccc acaaattatt
     8581 atcggtgcac aatacattca agctgctggt gttgcatttg cacttaaaaa acgtggtaaa
     8641 aatgcagttg caatcactta cactggtgac ggtggttctt cacaaggtga tttctacgaa
     8701 ggtattaact ttgcagcagc ttataaagca cctgcaattt tcgttattca aaacaataac
     8761 tatgcaattt caacaccaag aagcaagcaa actgctgctg aaacattagc tcaaaaagca
     8821 attgctgtag gtattcctgg tatccaagtt gatggtatgg atgcgttagc tgtatatcaa
     8881 gcaactaaag aagcacgtga ccgcgcagtt gcaggtgaag gtccaacatt aattgaaact
     8941 atgacatatc gttatggtcc tcatacaatg gctggtgacg atccaactcg ttacagaact
     9001 tcagacgaag atgctgaatg ggagaaaaaa gacccattag tacgtttccg taaattcctt
     9061 gaaaacaaag gtttatggaa tgaagacaaa gaaaatgaag ttattgaacg tgcaaaagct
     9121 gatattaaag cagcaattaa agaggctgat aacactgaaa aacaaactgt tacttctcta
     9181 atggaaatta tgtatgaaga tatgcctcaa aacttagcag aacaatatga aatttacaaa
     9241 gagaaggagt cgaagtaagc catggcacaa atgacaatgg ttcaagcgat taatgatgcg
     9301 cttaaaactg aacttaaaaa tgaccaagat gttttaattt ttggtgaaga cgttggtgtt
     9361 aacggcggtg ttttccgtgt tactgaagga ctacaaaaag aatttggtga agatagagta
     9421 ttcgatacac ctttagctga atcaggtatt ggtggtttag cgatgggtct tgcagttgaa
     9481 ggattccgtc cggttatgga agtacaattc ttaggtttcg tattcgaagt atttgatgcg
     9541 attgctggac aaattgcacg tactcgtttc cgttcaggcg gtactaaaac tgcacctgta
     9601 acaattcgta gcccatttgg tggtggcgta cacacaccag aattacacgc agataactta
     9661 gaaggtattt tagctcaatc tccaggtcta aaggttgtta ttccttcagg cccatacgat
     9721 gcgaaaggtt tattaatttc ttctattaga agtaatgacc cagtcgtata cttagagcat
     9781 atgaaattgt atcgttcatt ccgtgaagaa gtacctgaag aagaatatac aattgacatt
     9841 ggtaaggcta atgtgaaaaa agaaggtaat gacatttcaa tcatcacata cggtgcgatg
     9901 gttcaagaat caatgaaagc tgcagaagaa cttgaaaaag atggttattc tgttgaagta
     9961 attgacttac gtactgttca accaatcgat gttgacacaa ttgtagcttc agttgaaaaa
    10021 actggtcgtg cagttgtagt tcaagaagca caacgtcaag ctggtgttgg tgcagcagtt
    10081 gtagctgaat taagtgaacg tgcaatcctt tcattagaag cacctattgg aagagttgca
    10141 gcagcagata caatttatcc attcactcaa gctgaaaatg tttggttacc aaacaaaaat
    10201 gacatcatcg aaaaagcaaa agaaacttta gaattttaat acattttaaa agttaacgaa
    10261 gttagcgtat tttagtctca ttgattaaaa tgaaatgttt aatttacgaa atcttaggag
    10321 ggcaaaaacg tggcatttga atttagatta cccgatatcg gggaaggtat ccacgaaggt
    10381 gaaattgtaa aatggtttgt taaagctgga gatactattg aagaagacga tgttttagct
    10441 gaggtacaaa acgataaatc agtagtagaa atcccatcac cagtatctgg tactgtagaa
    10501 gaagttatgg tagaagaagg tacagtagct gtagttggtg acgttattgt taaaatcgat
    10561 gcacctgatg cagaagatat gcaatttaaa ggtcatgatg atgattcatc atctaaagaa
    10621 gaacctgcga aagaggaagc gccagcagag caagcacctg tagctactca aactgaagaa
    10681 gtagatgaaa acagaactgt taaagcaatg ccttcagtac gtaaatacgc acgtgaaaaa
    10741 ggtgttaaca ttaaagcagt ttctggatct ggtaaaaatg gtcgtattac aaaagaagat
    10801 gtagatgcat acttaaatgg tggtgcacca acagcttcaa atgaatcagc tgcttcagct
    10861 acaagtgaag aagttgctga aactcctgca gcacctgcag cagtaacatt agaaggcgac
    10921 ttcccagaaa caactgaaaa aatccctgct atgcgtagag caattgcgaa agcaatggtt
    10981 aactctaagc atactgcacc tcatgtaaca ttaatggatg aaattgatgt tcaagcatta
    11041 tgggatcacc gtaagaaatt taaagaaatc gcagctgaac aaggtactaa gttaacattc
    11101 ttaccttatg ttgttaaagc acttgtttct gcattgaaaa aatacccagc acttaacact
    11161 tcattcaatg aagaagctgg tgaaatcgtt cataaacatt actggaatat cggtattgca
    11221 gcagacactg atagaggatt attagtacct gttgttaaac atgctgatcg taagtctatt
    11281 ttccaaattt cagatgaaat taatgaatta gctgttaaag cacgtgatgg taaattaaca
    11341 gccgatgaaa tgaaaggtgc tacatgcaca atcagtaata tcggttcagc tggtggacaa
    11401 tggttcactc cagttatcaa tcacccagaa gtagcaatct taggaattgg ccgtattgct
    11461 caaaaaccta tcgttaaaga tggagaaatt gttgcagcac cagtattagc attatcatta
    11521 agctttgacc acagacaaat tgatggtgca actggccaaa atgcaatgaa tcacattaaa
    11581 cgtttattaa ataatccaga attattatta atggaggggt aaaacatggt agttggagat
    11641 ttcccaattg aaacagatac tatagtaatc ggagcaggtc ctggtggata cgttgcagca
    11701 attcgtgcag ctcaattagg acaaaaagta acaatcgttg agaaaggtaa tcttggtggt
    11761 gtttgcttaa acgtaggatg tattccttca aaagcattac tacatgcttc tcaccgtttt
    11821 gttgaagcac aacattctga aaacttaggt gttattgctg aaagtgtttc tttaaacttc
    11881 caaaaagttc aagaattcaa atcatcagtt gttaataaat taactggtgg tgttgaaggc
    11941 ttacttaaag gtaacaaagt taacatcgtt aaaggtgaag catatttcgt agataacaat
    12001 agcttacgtg ttatggacga aaagagcgca caaacataca actttaaaaa tggaatcatt
    12061 gcaacaggtt caagaccaat tgaaattcct aatttcaaat tcggtaaacg tgttatcgac
    12121 tcaacaggtg ctttaaactt acaagaagta ccaggtaaat tagttgtagt tggtggagga
    12181 tacattggat cagaattagg tacagcattt gctaactttg gttcagaagt aaccatcctt
    12241 gaaggtgcta aagatatctt aggtggcttc gaaaaacaaa tgacacaacc tgttaaaaaa
    12301 ggtatgaaag aaaaaggtgt tgaaatcgtt actgaagcta tggctaaatc agctgaagaa
    12361 acagataacg gagttaaagt tacttatgaa gctaaaggcg aagagaaaac aatcgaagct
    12421 gattatgtat tagtaactgt aggtcgtcgt ccaaacacag acgaattagg cctagaagaa
    12481 ttaggtgtta aattcgctga ccgtggatta ttagaagttg ataaacaaag ccgtacgtct
    12541 atcagcaata tctatgcaat tggtgatatc gttccaggtt taccacttgc tcacaaagct
    12601 agctatgaag ctaaagttgc tgctgaagca attgatggtc aagctgctga agttgattac
    12661 attggtatgc cagcagtatg ctttactgaa ccagaattag ctacagttgg ttattcagaa
    12721 gcgcaagcta aagaagaagg tttagcaatt aaagcttcta aattcccata tgcagcaaat
    12781 ggtcgtgcat tatcattaga tgatactaac ggatttgtta aacttattac acttaaagaa
    12841 gatgatactt taatcggtgc tcaagtagtt ggtactggtg catcagatat tatctctgaa
    12901 ttaggtttag caattgaagc tggtatgaat gctgaagata tcgcattaac aatccatgca
    12961 catccaacat taggtgagat gactatggaa gcagcagaaa aagctatcgg atacccaatc
    13021 catacaatgt aataactgat tatctataaa gattcagtca ttaaaagctg tagcatatgc
    13081 tacggctttt ttgttttagg taaagtaatg taaggaaatt gatttgagat atcgttaaca
    13141 tgtgacatgc atgttatact agcgatgcta acaaaagaat tgaaatggag ggttcaacaa
    13201 tggaatatga gtatccaatt gatttagact ggagtaatga agagatgatt tcagtgataa
    13261 atttctttaa tcatgtagag aagtattatg aatccggcgt gacggcaggc gactttatgg
    13321 gtgcatataa aagatttaaa gaaattgtgc ctgctaaagc agaggaaaaa caaattttta
    13381 atactttcga aaaaagtagt ggctataata gttacaaagc agttcaagat gtaaaaactc
    13441 actctgaaga acaaagagta acagctaaaa aataattcgt tcgaaattaa cacaatttaa
    13501 taggaatttt tctttaaaac tattgctaat aaagctatat tttgatacct ttatcaagtg
    13561 ttaaacaaaa tgtttgataa aagtaaactt aatatagctt ttttaggtgg aaaaataaat
    13621 gaacataggt aataaaatta aaaatcttag aagaattaaa aatttaacgc aagaagaact
    13681 tgctgaacgt acagacttat cgaaaggcta catttcacaa atagaaagtg aacatgcctc
    13741 accaagtatg gaaactttct taaatattat agaggtgtta ggaacgacgc caagtgaatt
    13801 ttttaaagac agtgaaaatg aaaaagtatt atacaagaag gaagaacaag ttatttatga
    13861 tgagtatgat gaaggttata tattaaattg gttagtttca aagtcaaatg aatatgatat
    13921 ggagccatta atattaactt taaagcctgg agcatcatat aaaaatttta atccatcaga
    13981 gtctgatacg tttatttatt gtatgtcagg tcagataaca cttaatttag gcaaagagat
    14041 atatcaagca caagaagaag acgttttgta ttttaaagca cgagataatc atcgtttgtc
    14101 aaacgaatca aacaatgaaa cacgaatact tattgtagcg acagcttcat atttataggg
    14161 gggatcttat ttggaaccgt tattatcatt aaaatcagtt agtaaaagct atgatgatct
    14221 taatatctta gatgacatag atattgatat tgaatcagga tacttttata cattattagg
    14281 tccttcaggt tgtggtaaaa caacaatttt aaaattaatt gcagggtttg aatatcctga
    14341 cagtggtgaa gtgatttatc aaaacaaacc aattggtaat ttaccaccaa ataaacgtaa
    14401 agtgaataca gtctttcaag attatgcatt atttccacac ttaaacgtct atgataatat
    14461 cgcttttggt ttgaaattaa aaaaattatc aaaaaccgaa attgatcaaa aagtaactga
    14521 ggcattaaaa ttagtaaaac tttcaggtta tgaaaaaaga aatattaatg aaatgagtgg
    14581 cggacaaaag caacgtgttg caattgcacg tgctatcgta aatgaaccag aaatattatt
    14641 gttagatgaa tctttatccg cattagattt gaaattgcgt actgaaatgc aatatgaatt
    14701 acgagaattg caatctagat taggtattac atttatattt gtaacacatg atcaagaaga
    14761 agcgttagca ttaagtgact ttctttttgt attaaaagat gggaaaattc aacaatttgg
    14821 cacaccaaca gatatatatg acgaaccagt gaatcgattt gtagctgatt ttattggaga
    14881 atctaatatt gttgaagggc gcatggttag agattatgtc gtgaatattt atgggcaaga
    14941 tttcgaatgt gtcgatatgg gtattcctga aaataaaaaa gtagaagtcg ttattcgacc
    15001 agaagatata tcattaatca aagctgaaga aggattattt aaagcaactg ttgattctat
    15061 gttatttaga ggggtccact atgaaatatg ttgtatagac aataaaggtt atgaatgggt
    15121 aatacaaacg actaaaaaag ctgaagtagg cagtgaagtt ggtctttatt ttgatcctga
    15181 agccattcat attatggttc ctggagaaac agaagaagaa tttgataaac gtattgaaag
    15241 ctatgaggaa gtagacaatg cgtaatacta ataaatttct cttaatcccg tatttactat
    15301 ggatggttat atttattatt gtaccagttg tattactcat ttatttttca tttttagata
    15361 tcaatggaca ttttagtttc acgaattatc aacaaatttt tactacaaaa tatttgaaaa
    15421 tgtttgcata ttcaatttta tatgccgctt caataacaat tattaccttg actatcagtt
    15481 atccagctgc ctattatatt actcgttcga aatttcaaaa tatcttatta atgataatga
    15541 ttattccaac atggataaat ttattgttaa agacatatgc ttttataggt ttattaagtc
    15601 atgatggcgt gattaatcaa tttttccact tatttaattt accatcattc aatttgttat
    15661 ttacaactgg tgcgttttta gtagtggcaa gttacattta tataccattt atgattttac
    15721 ctatatttaa tagcatgaaa gcaattccta ataatttatt gcaggcctca agtgatttag
    15781 gtgccagtcc tttctatact tttagaaaag taatcatgcc gttaacaaaa gaaggtgtta
    15841 tgactgggat tcaagtaacc tttattccat cactttcatt atttatgatt acaagattaa
    15901 ttgcaggtaa taaagtcata aatataggta cggcaataga ggaacaattt ttaacaattc
    15961 aaaattatgg tatgggatca actatagcta tattcttaat tgtatttatg gcattcattt
    16021 taatcattac aaaatcatct aatgggagag ggtgaatcat atgaaatggt atggaaagct
    16081 gtatatcggg atacttttag cgattttata catcccaata ttctttttaa tgttctattc
    16141 attcaattcg gctggtaata tgattcactt tgaacatttt acattagagc attatcaatc
    16201 attatttcaa aatgatcatt taatgtcggt catttttaat acgatagctg tagcactttt
    16261 agcagcctca atttctacag ttattggtac atttggtgcc attgctattt attatttaag
    16321 aaataaaaag tttaaagtaa ctttactaac attgaataat gtcttgatgg tatcatccga
    16381 cgttgtcata ggtgcatcat tcttaattat gtttacaacg attggccatt ttactggtct
    16441 tggtttagga ttttggacag ttctaatatc tcatattgca ttttgcatac ctatagttgt
    16501 gattatcgtc ttaccacaac tgtatgaaat gaataataat atgttaaatg ctgcaagaga
    16561 tttaggagcg actgaaccac aattattaag caacattatt attcctaata ttttaccttc
    16621 tattatagga ggattcttta tggctttaac ttattcacta gacgatttca cagtaagttt
    16681 cttcgttact ggtaatggct ttagtgtgtt atcagttgaa gtttatgcta tggcgagaaa
    16741 aggaattagt atggaaatta atgcgatttc aacattattg tttgctgtta ttgtattagg
    16801 aatactagga tattatttga ttcaatacgt gataaataag aaaaaactaa tcaagcgagg
    16861 tgtaaaataa tgaaacgatt tttacaactc attataggtg cattagttgt gggtatgctt
    16921 tgtcttactt taagtcattg gtttaaatct aaagaacaag tgcatacaaa tcaaaaaatt
    16981 tacgtataca attggggcga atatattgat ccagagttaa ttaagaaatt tgaaaaagaa
    17041 actggcattc aagtcgttta tgaaactttc gattcaaatg aagcgatgga agccaaaatt
    17101 cgcaatggcg gtacacatta tgatgttgct tttcctagtg aatatacagt tcaaaaattg
    17161 aaaagagatc atttattgtt ac
//
LOCUS       DAKTGS010000050        17084 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.050, whole genome shotgun sequence.
ACCESSION   DAKTGS010000050 DAKTGS010000000
VERSION     DAKTGS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17084
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.050"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..657
                     /gene="capA"
                     /locus_tag="O0286_001369"
     CDS             <1..657
                     /gene="capA"
                     /locus_tag="O0286_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCY0169753.1"
                     /translation="LELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANT
                     QILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSM
                     LTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTA
                     VKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN
                     "
     gene            673..1359
                     /gene="cap8B"
                     /locus_tag="O0286_001370"
     CDS             673..1359
                     /gene="cap8B"
                     /locus_tag="O0286_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCY0169754.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1362..2126
                     /gene="cap8C"
                     /locus_tag="O0286_001371"
     CDS             1362..2126
                     /gene="cap8C"
                     /locus_tag="O0286_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCY0169755.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2146..3970
                     /gene="cap8D"
                     /locus_tag="O0286_001372"
                     /pseudo
     CDS             2146..3970
                     /gene="cap8D"
                     /locus_tag="O0286_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            3960..4988
                     /gene="cap8E"
                     /locus_tag="O0286_001373"
     CDS             3960..4988
                     /gene="cap8E"
                     /locus_tag="O0286_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCY0169756.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            5001..6110
                     /gene="cap8F"
                     /locus_tag="O0286_001374"
     CDS             5001..6110
                     /gene="cap8F"
                     /locus_tag="O0286_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCY0169757.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6114..7238
                     /gene="cap8G"
                     /locus_tag="O0286_001375"
     CDS             6114..7238
                     /gene="cap8G"
                     /locus_tag="O0286_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCY0169758.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7241..7867
                     /locus_tag="O0286_001376"
     CDS             7241..7867
                     /locus_tag="O0286_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCY0169759.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7872..8981
                     /locus_tag="O0286_001377"
     CDS             7872..8981
                     /locus_tag="O0286_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0169760.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            8995..10161
                     /locus_tag="O0286_001378"
     CDS             8995..10161
                     /locus_tag="O0286_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0169761.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10154..11359
                     /locus_tag="O0286_001379"
     CDS             10154..11359
                     /locus_tag="O0286_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCY0169762.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            11360..12565
                     /gene="cap8L"
                     /locus_tag="O0286_001380"
     CDS             11360..12565
                     /gene="cap8L"
                     /locus_tag="O0286_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCY0169763.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            12576..13133
                     /gene="cap8M"
                     /locus_tag="O0286_001381"
     CDS             12576..13133
                     /gene="cap8M"
                     /locus_tag="O0286_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCY0169764.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            13133..14020
                     /gene="capN"
                     /locus_tag="O0286_001382"
     CDS             13133..14020
                     /gene="capN"
                     /locus_tag="O0286_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCY0169765.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            14074..15336
                     /gene="cap8O"
                     /locus_tag="O0286_001383"
     CDS             14074..15336
                     /gene="cap8O"
                     /locus_tag="O0286_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCY0169766.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            15413..16558
                     /gene="cap8P"
                     /locus_tag="O0286_001384"
     CDS             15413..16558
                     /gene="cap8P"
                     /locus_tag="O0286_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCY0169767.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(16623..16949)
                     /gene="isdI"
                     /locus_tag="O0286_001385"
     CDS             complement(16623..16949)
                     /gene="isdI"
                     /locus_tag="O0286_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCY0169768.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(16956..>17084)
                     /locus_tag="O0286_001386"
     CDS             complement(16956..>17084)
                     /locus_tag="O0286_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 domain-containing protein"
                     /protein_id="HCY0169769.1"
                     /translation="FSIYMVDVLAVRVGLIIMVIIQTAVLFTFVKTLPKSNHKIEE"
BASE COUNT         6085 a         2398 c         3207 g         5394 t
ORIGIN      
        1 ttagaattaa caaaaattaa agaagtatta caaaaaaact tgaagatttt aattatttta
       61 ccgctattat ttttaattat tagcgctatt gttacatttt tcgtcttatc acctaaatat
      121 caagctaata ctcaaatttt agtgaatcaa actaagggtg acaatcctca gtttatggcg
      181 caagaggttc aaagtaatat tcaacttgta aatacgtata aagaaattgt taaaagtcct
      241 agaattttag atgaggtgtc aaaggactta aatgataagt attcaccatc taaattgtcg
      301 agtatgttga caattacaaa ccaagaaaat acgcaactta tcaacatcca agttaaaagt
      361 ggtcataaac aagattcgga aaaaattgcg aatagcttcg ctaaagttac aagtaaacaa
      421 attccgaaga ttatgagtgt ggataacgta tcaattttat ctaaagcaga cggtacagca
      481 gttaaagtcg caccaaaaac tgtagtgaat ctaatcggtg cattcttttt aggattagtt
      541 gtcgcgctta tatatatctt cttcaaagta attttcgata agcgaattaa agatgaagaa
      601 gatgtagaga aagaattagg attgcctgta ttgggttcaa ttcaaaaatt taattaagga
      661 tggttgctac ttatgtcaaa aaaggaaaat acgacaacaa cactatttgt atatgaaaaa
      721 ccaaaatcaa caattagtga aaagtttcga ggtatacgtt caaacatcat gttttcaaaa
      781 gcaaatggtg aagtaaagcg cttattggtt acttctgaaa agcctggtgc aggtaaaagt
      841 acagttgtat cgaatgtagc gattacttat gcacaagcag gctataagac attagttatt
      901 gatggcgata tgcgtaagcc aacacaaaac tatattttta atgagcaaaa taataatgga
      961 ctatcaagct taatcattgg tcgaacgact atgtcagaag caattacgtc gacagaaatt
     1021 gaaaatttag atttgctaac agctggccct gtacctccaa atccatctga gttaattggg
     1081 tctgaaaggt tcaaagaatt agttgatctg tttaataaac gttacgacat tattattgtc
     1141 gatacaccgc cagttaatac tgtgactgat gcacaactat atgcgcgtgc tattaaagat
     1201 agtctgttag taattgatag tgaaaaaaat gataaaaatg aagttaaaaa agcaaaagca
     1261 cttatggaaa aagcaggcag taacattcta ggtgtcattt tgaacaagac aaaggtcgat
     1321 aaatcttcta gttattatca ctattatgga gatgaataag tatgattgat attcataacc
     1381 atatattgcc taatatcgat gacggtccga caaatgaaac agagatgatg gatcttttaa
     1441 aacaagcgac aacacaaggt gttacagaaa tcattgtaac atcacatcac ttacatcctc
     1501 gatataccac acctatagaa aaagtgaaat catgtttaaa ccatattgaa agcttagagg
     1561 aagtacaagc actaaatcta aagttttatt atggtcagga aataagaatt accgatcaaa
     1621 tccttaatga tattgatcga aaagttatta acggtattaa tgattcacgc tatttactaa
     1681 tagaatttcc atcaaatgaa gttccacact atactgatca attatttttc gaattacaga
     1741 gtaaaggctt tgtaccgatt attgcacatc cagagcggaa taaagcaata agtcaaaacc
     1801 ttgacatact atacgattta attaacaaag gtgctttaag tcaagtgaca acggcgtcat
     1861 tagcgggtat ttccggtaaa aaaattagaa aattagcaat tcaaatgatt gaaaacaatc
     1921 tgacacattt catcggttca gatgcgcata acacagaaat cagaccgttc ttaatgaaag
     1981 acttatttaa tgataagaaa ttacgtgatt attatgaaga tatgaacgga tttattagta
     2041 atgcgaagtt agttgttgat gataaaaaaa ttcctaaacg aatgccacaa caagattata
     2101 aacagaaaag atggtttggg ttataaacag caaatgaggg gttttatggc acatttatct
     2161 gtgaaattgc ggcttttaat actagcatta atcgattcac tgatagtgac attttcagta
     2221 ttcgtaagtt attacatttt agaaccgtat ttcaaaacat attctgtcaa attattaata
     2281 ttggcagcta tatcactatt catatcgcat catatttcag catttatttt taatatgtat
     2341 catcgagcgt gggaatatgc cagtgtgagt gaattgattt taattgttaa agctgtgacg
     2401 acatctatcg ttattacgat ggtggtcgtg acaattgtta caggcaatag accgtttttt
     2461 agattgtatt taattacttg gatgatgcac ttgattttaa taggtggctc aaggttattt
     2521 tggcgtattt atcggaaata ccttggaggt aagtcattta ataagaagcc aactttagtt
     2581 gttggtgctg gtcaagcagg ttcaatgctg attagacaaa tgttgaaaag tgacgaaatg
     2641 aaacttgaac cggtattagc agtcgatgat gacgaacata aacgcaatat cacaattact
     2701 gagggtgtaa aagtccaagg taaaattgcg gatattccag aactagtgag gaaatataag
     2761 attaaaaaaa tcatcattgc aattccaact attggtcaag agcgtttgaa agaaattaat
     2821 aatatttgcc atatggatgg cgttgagtta ttgaaaatgc caaatataga agacgtcatg
     2881 tctggtgagt tagaagtgaa ccaacttaaa aaagttgaag tagaagattt actaggcaga
     2941 gatcctgttg aattagatat ggatatgata tcaaatgaat tgacgaataa aactatttta
     3001 gttacgggtg caggtggttc aataggatca gaaatttgta gacaagtttg taatttctat
     3061 ccagaacgta ttattctact tggccatggt gaaaacagta tttatttaat caatcgtgaa
     3121 ttgcgaaatc gcttcggaaa aaaatgttga tatcgttcct attatagcgg atgtgcaaaa
     3181 tagagcgcgt atgtttgaaa ttatggaaac gtataaacca tacgcagttt atcatgcagc
     3241 agcacacaag cacgtgccgt taatggaaga caaccctgaa gaagcagtac gtaataatat
     3301 tttaggtacg aaaaatactg ctgaagctgc taaaaatgca gaggtaaaga aattcgttat
     3361 gatttctacg gataaagccg ttaatccgcc taatgtcatg ggagcttcaa agcgaattgc
     3421 agaaatgatt attcaaagtt taaatgatga aacgcatcga acaaattttg ttgcagtgag
     3481 atttggtaat gtacttggat cgagaggatc tgtgattcca cttttcaaaa gtcaaattga
     3541 agaaggtggg ccagttactg tgacacatcc tgaaatgaca cgttacttta tgacaattcc
     3601 tgaagcttct agactagttt tgcaggcagg ggcattagca gaaggtggcg aagtatttgt
     3661 gctagatatg ggagaaccag tgaaaattgt agatttggca cgtaatttaa ttaagctaag
     3721 tggtaaaaaa gaagacgaca tacgcattac ttatacaggg attagacccg gcgaaaaaat
     3781 gtttgaagag cttatgaata aagatgaggt tcatcctgaa caagtatttg aaaaaattta
     3841 tcgtggcaaa gtacaacata tgaaatgtaa tgaagttgaa gcgattattc aagacatcgt
     3901 caatgacttt agtaaagaaa aaattattaa ctatgccaat ggcaaaaagg gagataatta
     3961 tgttcgatga caaaatttta ttaattactg ggggcacagg atcattcggt aatgctgtta
     4021 tgaaacagtt tttagattct aatattaaag aaattcgtat tttttcacgc gatgagaaaa
     4081 aacaagatga cattcgaaaa aaatataata attcaaaatt aaagttctac attggtgatg
     4141 tgcgtgatag tcaaagtgta gaaacagcaa tgcgagatgt ttattacgta ttccatgcag
     4201 cagctttaaa acaagtgccg tcatgtgaat tctttccagt tgaggcagtg aagacaaata
     4261 ttattggtac agaaaatgtc ttacaaagtg ctattcatca aaatgttaaa aaagtcatat
     4321 gtttatctac agataaggca gcgtatccta ttaatgctat gggtatttca aaagcaatga
     4381 tggaaaaagt attcgtagcc aaatcaagaa atattcgtag tgaacaaacg cttatttgtg
     4441 gtacaagata cggtaatgtg atggcttcaa gaggatcagt aatacctttg tttatcgaca
     4501 aaatcaaagc tggagaacct ttaacgatta cagatcctga tatgacaaga tttttaatga
     4561 gcttagaaga tgcggtagaa ctagttgttc atgcatttaa gcatgcagag acaggagata
     4621 ttatggttca aaaagcacca agctcaacgg taggggatct tgcgaccgca ttattagaat
     4681 tgtttgaagc tgataatgca attgaaatca ttggtacgcg acatggagag aaaaaagcag
     4741 aaacattgtt gacgagagaa gaatacgcac aatgtgaaga tatgggtgat tattttagag
     4801 tgccggcaga ctccagagat ttaaattata gtaattatgt tgaaaccggt aacgaaaaga
     4861 ttacgcaatc ttatgaatat aactccgata atacacatat tttaacggtg gaagagataa
     4921 aagaaaaact tttaacacta gaatatgtta gaaacgaatt gaatgattat aaagcttcaa
     4981 tgagatagga gagattgacg ttgaatattg taattacagg agcaaaaggt tttgtaggaa
     5041 aaaacttgaa agcagattta acttcaacga cagatcatca tattttcgaa gtacatcgac
     5101 aaactaaaga ggaagaatta gagtcagcat tgttgaaagc agactttgtc gtgcatttag
     5161 cgggtgttaa tcgacctgaa catgacaaag aattcagctt aggaaacgtg agttatttag
     5221 atcatgtact tgatatatta actagaaata cgaaaaagcc agcgatatta ttatcgtctt
     5281 caatacaagc aacacaagat aatccttatg gtgagagtaa gttgcaaggg gaacagctat
     5341 taagagagta tgccgaagag tatggcaata cggtttatat ttatcgctgg ccaaatttat
     5401 tcggcaagtg gtgtaagccg aattataact cagtgatagc aacattttgt tacaaaattg
     5461 cacgtaacga agagattcaa gttaatgatc ggaatgttga actaacgcta aactacgtgg
     5521 atgatatcgt cgctgaaata aagcgtgcta ttgaaggaac tccaacgatt gaaaatggtg
     5581 tacctacagt accaaacgta tttaaagtga cattgggaga aattgtagat ttattataca
     5641 agttcaaaca gtcacgtctc gatcgaacat tgccgaaatt agataacttg tttgaaaaag
     5701 atttgtatag tacgtattta agctatctac ctagtacaga ctttagttat cccttactta
     5761 tgaatgtgga tgataggggt tcttttacag aatttataaa aacaccggat cgtggtcaag
     5821 tttctgtaaa tatttctaaa ccaggtatta ctaaaggtaa tcactggcat catactaaaa
     5881 acgaaaaatt tctagtcgta tcaggtaaag gggtaattcg ttttagacat gttaatgatg
     5941 atgaaatcat tgaatattat gtttctggcg acaaattaga agttgtagac ataccagtag
     6001 gatacacaca taatattgaa aatttaggcg acacagatat ggtaactatt atgtgggtga
     6061 atgaaatgtt tgatccaaat cagccagata cgtatttctt ggaggtatag cgcatggaaa
     6121 aactgaaatt aatgacaata gttggtacaa ggcctgaaat cattcgttta tcatcaacga
     6181 ttaaagcatg tgatcaatat tttaatcaga tattagtaca cactggtcaa aattatgatt
     6241 atacattgaa tcaaattttc tttgatgatt tggaattaag acaaccggac cactacttag
     6301 aggcagttgg aagtaacctt ggagaaacga tggggaatat tattgcgaag acatatgatg
     6361 ttttattacg cgaacaacca gatgcacttt taattcttgg tgatacaaat agttgtttag
     6421 cagcagtatc tgctaaacga ttaaagattc ctgtgttcca catggaagcg ggtaatagat
     6481 gctttgatca gaatgtacct gaagaaatca atcgtaaaat tgttgaccat gtcagtgatg
     6541 tgaatctacc ttatacggaa catagcagac gttatttatt agatgaaggc ctcaataaag
     6601 cgaatatctt tgtgacagga tcaccgatga cagaagtgat agaagcgcat cgagataaaa
     6661 ttaatcacag tgacgtttta aataaactag gattagaacc gcaacaatac attttagtat
     6721 ctgcgcatag agaagagaat atcgataatg aaaagaattt taaatcatta atgaatgcga
     6781 taaatgatat tgccaaaaag tataaaatgc ctgtgattta ttcaacgcat ccaagaagtt
     6841 ggaagaaaat tgaagaaagt aaatttgaat ttgatccatt agttaaacag ttaaagccat
     6901 ttggtttctt tgattataat gcattgcaaa aagatgcatt tgttgtgcta tcagatagtg
     6961 gaacattgtc agaagagtcg tctattttga agttccctgg tgtccttatt cgaacttcca
     7021 cagaaagacc ggaagtacta gataaaggta cggttattgt aggtggtatt acctataaca
     7081 atctaatcca atccgttgaa ctagcaagag agatgcaaaa caataacgaa ccgatgattg
     7141 atgctattga ttataaagac actaacgttt cgacaaaggt agttaaaatt attcaaagct
     7201 ataaagatat tatcaatcga aatacttgga ggaaatgacg atgaggatag cgattgaaaa
     7261 gataattggt ttgctgaaaa accagtcctc taaagaatcg aatgttaaga ttcatcgctt
     7321 ggcgtatatt acaaactcaa aatttgatgg caataactat atagatagat ggtgtaaaat
     7381 caggaattct cacattggtg aatacagtta tattggattt ggtagtgatt ttaataatgt
     7441 agaagtagga agatattgtt cgatatcttc ggatgtaaaa attgggttag gaaaacatcc
     7501 tacacacttt tttagctcat caccgatttt ttattctaat aataatccat ttaacataaa
     7561 gcaaaagttt atagacttta atgaccaacc aagccgtaca acaattaaaa atgatgtgtg
     7621 gattggtgca aatgtaatta ttatggatgg tttaacaata aatactggtg cagtcatagc
     7681 agccggctca gttgttacta aaaatgtagg agcatatgag gttgttggtg gtgttcctgc
     7741 aaaagtgatt aagaagcgat ttgacaataa aacaattgaa aaacttttgg aaagcaagtg
     7801 gtgggagaaa acgcctgaca aactaaaagg attttcggtt gaatatttaa ataaaaagga
     7861 tacttaatga tatgagaatt ttaaatattg tatcgagtaa tattgttcaa gacccaaggg
     7921 tacttaaaca aatagaaaca attaaaggcg ttacggatga ttataaaatt gttggaatga
     7981 ataattcaca agctactaat aagcgattgg aaaatttaga ttgtaattat cgtttgttag
     8041 gtagcaaggt agatccaaaa aatattcttt ctaaattaat taagcgtata agatttgcaa
     8101 caggtgttat ccgagaaatt aaagcttata aacctgacgt gattcatgca aatgatttcg
     8161 acgtattatt aatggtctat ttaagcaatt ataaaaaagc taatattgtt tatgatgcgc
     8221 atgaaatata tgcgaaaaat gcctttatta ataaagttcc acttatttca aagtttgtag
     8281 aaagtataga aaaacacata gtaaaacatc gtgttaatgc cttcgtaaca gtaagtcatg
     8341 cagcaaaaga atattatcaa tctaaaggat ataagaagga agcgaatgtt attacgaatg
     8401 cacctatttt aaatgatagc agagaattta aagaaatcga aaactttaaa gaaattgtat
     8461 atcaaggtca aattgtaatg gacagaggat atgaagagtt tattattgct tcatcagctt
     8521 ttaaacaaaa tgctccttca ttcataattc gagggtttgg tccgcatgaa gaagtgataa
     8581 aagaactgat tagttataac ccggaaaata ttaggttgga taaaccagtt gaagtaaaag
     8641 aattggttga taagttagca gaaagtaatg ttggtgttgt cttgacgaaa ccagtatcta
     8701 ttaattttga atatacagta tctaataaaa tttttgaatg tatacatgct ggtttaccag
     8761 taattttatc tcctgtcaaa gagcatattt atctcaatga aaaatataaa tttggcattg
     8821 ttctaaagga agttacgccg ttagaaattg aaaaggcggt tagaaaatta agagataatc
     8881 acgatttgtt taatcattta cgtcaaaatg caattaaggc gtctaaaatt ttgaattggc
     8941 aaatagagag tgaacggtta gtagaattat ataaatttta aagagaggta gactatgaaa
     9001 ttttttgtac tttgtgcaat tatcagcatg aacatattta tagtaatctc tacatttact
     9061 aaagaagtat tagggttccc tatagagccg gtgtattact caaccatggt tggtatagca
     9121 ttaattacta cggtgtttgc tatttataag ataattgtca cccaagaaat tccgcgaggg
     9181 ttaatattat taattgctat atgtttgctt tatctagctt tttattattt ttcaccagat
     9241 aaggaagaga aactagctaa aaataatatt ctattctttt taacatgggc agttccagcg
     9301 gcaattagtg gtatttatat taaatatata aacaaggcta cggtagaaag attttttaaa
     9361 ttagtatttt tcatattttc tgtttcattt atttttgtaa ttttaatacc aaaacttaca
     9421 ggtgagatac ctagctatat caattttgga cttatgaact atcaaaacgc ttcgtacctt
     9481 tcagcattta ctgccggatt aggcatttat ttcattatga aaggttcagt taaacataag
     9541 tggatatatg ttctatttac aataattgat atccctattg tgtttatacc aggagggcgt
     9601 ggaggtgcta ttttattaat tctttacggc ttatttgcat ttatacttat tacgtttaaa
     9661 agaggaatac ctatcgcagt aaaaagcatt atgtatattt ttgcattaag catatctagt
     9721 gtattgattt actttctttt tacaaaaggt tcgaatacta gaacattttc atatctacaa
     9781 ggtggaacac ttaatttaga aggtacttct ggaagaggac cgatttatga aaaaggtatt
     9841 tactttattc aacaaagttc gttattaggc tatgggccat ttaactatta taaactaatc
     9901 ggaaatatac cacataacat cattattgag ttgattctat catttggctt attagggttt
     9961 tttatcataa tgatttgcat tttgctacta gtttataaaa tgattaggaa ctatgatcca
    10021 aacactatag atttactcgt tatgtttata gcaatctatc caatcacatt attaatgttt
    10081 agttcaaatt atttagttgt aagtgaattt tggtttgtgt tgttctattt tattacaaaa
    10141 ggacggcgtc atcatggcta agaaagtttt tattatggat agcgtaaaga caataattgg
    10201 tacgttgctt atagctttag gattacaatt tttagcttat ccaattatta atcaacgagt
    10261 aggtaatgaa gcgttcggtt ctattttaac gatttataca ataataacaa tcacgagtgt
    10321 tgtattaggc aatacgctta acaatatacg attgattaat atgaatctat acaaatccaa
    10381 tcattactac tggaaatttg catcgatact tttaatctca attctgattg agagtatagc
    10441 tttaattatt gtatttcttt acttttttaa tttgaacatc atcgatatta tctttttaat
    10501 tctacttaat attttaatgt gtttaaggat ttatctgaat gtatttttta ggatgacttt
    10561 aaaatataat cagattttgt atattgctct tattcaattt ttaggtttgc tgataggact
    10621 atttctatat tatttaaccc aaaactggat tgtttgtttt attaccagtg aattgtttgc
    10681 aacgatatat acattggtta aattacgggg attaactata ggcgagtatc aaagtgaaga
    10741 taataatgtg gtaaaagatt atgtgatgct actgagtaca aatagcctta ataatttgaa
    10801 tctctattta gatagattaa tcttattacc aattataggt ggaacagctg taactatatc
    10861 atttctttca acatttattg ggaaaatgtt agctacattt ctatatccga ttaataatgt
    10921 agtactttca tatatttctg taaatgaaag tgacaatata aagaagcaat atttgaaaac
    10981 taatctaatt gctatagctg ccctatgttt agtcatgatt atatgttatc caattacaat
    11041 aattattgtc tctttactgt ataacattga ttcaagttta tattcgaagt ttattatttt
    11101 aggtaatata ggtgttttat tcaatgcagt gagtattatg atccaaactt taaatacaaa
    11161 acacgcatca ataacattac aagcgaatta tatgacgctt cacacgatta catttatatt
    11221 cataactatt ttaatgacaa ttgcgtttgg tctaaatgga ttcttttgga caacgctgtt
    11281 cagcaacatt attaagtatg tgattttaaa tattataggt ttaaagtcta aattcattaa
    11341 taaaaaggac gtcgattaga tgagtgaaaa aaagattttg attttatgtc agtattttta
    11401 tccggaatat gtatcttctg cgacgttacc aactcaattg gcggaagatt taattgcgaa
    11461 tcacattaat gtcgatgtca tgtgtggatg gccatatgaa tatagtaatc ataaacaggt
    11521 ttctaaaacc gagatgcatc gtggtattcg cattcgacgt ctcaagtatt cgaggtttaa
    11581 taacaaaagt aaggttggaa ggatcatcaa tttctttagt ttattttcaa aattcgtgat
    11641 taatatacct aaaatgttga aatatgatca gattcttgtt tactctaatc caccaatctt
    11701 gccattaata ccagacgttt tacacagact gcttaagaaa aaatattctt ttgtggtgta
    11761 tgatatagca cctgataatg cgattaagac aggtgcaact cgtccaggta gcatgattga
    11821 taagctgatg cgttacatta atagacatgt ctacaagaat gctgaaaatg tcattgtcct
    11881 tggtacggaa atgaaaaact acttactaaa tcatcaaatt tctaaaaatg ctgacaatat
    11941 ccatgtgatt cctaactggt atgacatgcg tcaattacaa gacaatcgta tctataatga
    12001 cacatttaaa gcttaccgtg agcaatacga caaaatttta ttgtatagcg gtaatatggg
    12061 gcagttacag gatatggaga cacttatctc atttttaaaa ttaaataagg atcagtctca
    12121 aacgttaaca atactttgtg gtcatggtaa gaaatttgca gatgtcaaaa cggcaataga
    12181 agaccatcgt attgaaaatg ttaaaatgtt tgagttttta acaggtacag actatgctga
    12241 cgtattaaaa attgcggatg tatgtattgc atcgctgatt aaagaaggcg tcggtttagg
    12301 cgtgccgagc aagaattatg gctatcttgc agctaagaaa gcgttggtac tcatcatgga
    12361 taagcaatct gatatcgttc aacatgttga acaatatgat gcgggtatcc aaattgataa
    12421 tggcgatgca catgccattt ataacttcat caacactcac tcgagtaagg aattgcacga
    12481 gatgggtgag cgcgcacatc aactgtttaa agataaatat acgagagaaa ttaatactat
    12541 gaagtattac aatctgttga agtgaggaga taattatgaa gcgattattc gatgtagtga
    12601 gttcaatata tggtttagta gttttaagtc cgattctgtt aattacagca ttactaatta
    12661 aaatggaatc acctggacca gccattttca aacaaaaaag accgacgatt aataatgaat
    12721 tgtttaatat ttataagttt agatcaatga aaatagacac acctaatgtt gcaactgatt
    12781 taatggattc aacatcgtat ataacaaaga cagggaaggt cattcgtaag acctctattg
    12841 atgaattgcc acaattattg aatgttttaa aaggagaaat gtcaattgta ggtcctagac
    12901 cagcgcttta taatcaatac gaattaatcg aaaaacgtac aaaagcgaac gtgcatacga
    12961 ttagaccagg tgtgacagga ctagctcaag tgatggggag agatgatatc actgatgatc
    13021 aaaaagtagc gtatgatcat tattacttaa cacatcaatc tatgatgctt gatatgtata
    13081 tcatatataa aacaattaaa aatatcgtta cttcagaagg tgtgcatcac taatgagaaa
    13141 aaatatttta attacaggcg tacatggata tatcggtaat gctttaaaag ataagcttat
    13201 tgaacaagga catcaagtag atcaaattaa tgttaggaat caattatgga agtcgacctc
    13261 gttcaaagat tatgatgttt taattcatac agcagctttg gttcacaaca attcacctca
    13321 agcaaggcta tctgattata tgcaagtgaa tatgttgctg acgaaacaat tggcacaaaa
    13381 ggctaaagct gaagacgtta aacaatttat ttttatgagt actatggcag tttatggaaa
    13441 agaaggtcat gttggtaaat cagatcaagt tgatacacaa acaccaatga accctacgac
    13501 caactatggt atttccaaaa agttcgctga acaagcatta caagaattga ttagtgattc
    13561 gtttaaagta gcaattgtga gaccaccaat gatttatggt gcacattgcc caggaaattt
    13621 ccaacggtta atgcaattgt caaagcgatt gccaatcatt cccaatatta acaatcagcg
    13681 cagtgcatta tatattaaac atctgacagc atttattgat caattaatat cattagaagt
    13741 gacaggtgtg taccatcctc aagatagttt ttactttgat acatcgtcag taatgtatga
    13801 aatacgtcgc caatcacatc gtaaaacggt attgatcaac atgccttcaa tgctaaataa
    13861 gtattttaat aagttgtcgg tctttagaaa attattcggc aatttaatat acagcaatac
    13921 gttatatgaa aataataatg cacttgaaat tattcctgga aaaatgtcac ttgttattgc
    13981 ggacatcatg gatgaaacga caaccaaaga taaggcataa gtcatctatt aaataaaatc
    14041 aacatacaaa tcgttttatt tggaggttat agtatgaagt taacagtagt tggcttaggt
    14101 tatattggtt taccaacatc aattatgttt gcaaaacatg gcgtcgatgt gcttggtgtt
    14161 gatattaatc agcaaacgat tgataagtta caaagtggtc aaattagtat tgaagaacct
    14221 ggattacaag aggtttatga agaggtactg tcatcgggaa aattgaaggt atctacaacg
    14281 ccagatgcat ctgatgtttt tatcattgcc gttccgacgc cgaataatga tgatcagtac
    14341 cggtcatgtg acatttcgct agttatgcgt gcattagata gtattttatc atttttagaa
    14401 aaaggaaata ccattattgt agagtcgaca attgcgccta aaacgatgga tgattttgta
    14461 aaaccagtca ttgaaaattt agggtttaca ataggtgaag atatttattt agtgcattgt
    14521 ccagaacgtg tactgccagg aaaaatttta gaagaattag ttcataacaa tcgtatcatt
    14581 ggcggtgtga ctgaagcttg tattgaagcg ggtaaacgtg tctatcgcac attcgttcag
    14641 ggagaaatga ttgaaacaga tgcacgtact gctgaaatga gtaagctaat ggaaaacaca
    14701 tatagagacg tgaacattgc tttagctaat gaattaacaa aaatttgcaa taacttaaat
    14761 attaatgtat tagatgtgat tgaaatggca aacaaacatc cgcgtgttaa catccatcag
    14821 cctggtccag gtgtaggcgg tcattgttta gctgttgatc cgtactttat tattgctaaa
    14881 gaccctgaaa atgcaaagtt aattcaaact ggacgtgaaa ttaataattc aatgccggcc
    14941 tatgttgttg atacaacgaa gcaaatcatc aaagtgttga gcgggaataa agtcacagta
    15001 tttggtttaa cttataaagg tgatgttgat gatataagag aatcaccagc atttgatatt
    15061 tatgagctat taaatcaaga accagacata gaagtatgtg cttatgatcc acatgttgaa
    15121 ttagattttg tggaacatga tatgtcacat gctgtcaaag acgcatcgct agtattgatt
    15181 ttaagtgacc actcagaatt taaaaattta tcggacagtc attttgataa aatgaagcat
    15241 aaagtgattt ttgatacaaa aaatgttgtg aaatcatcat ttgaagatgt atcgtattat
    15301 aattatggca atatatttaa ttttatcgac aaataaaatg tgtcaaacta gggcatacat
    15361 gattaaggaa agataagctg tcatgtgttt gaacttcaga gaggataatg ttatgaaaaa
    15421 aattatggtt attttcggta cgagacccga agcaataaaa atggcaccat tagtaaaaga
    15481 aattgatcat aatgggaact ttgaagcgaa cattgtgatt acagcacaac atagagatat
    15541 gttagatagt gtgttaagta tatttgatat tcaagctgat catgatttaa atattatgca
    15601 agatcaacaa acattagcag gccttacggc gaatgcactt gctaaacttg atagcatcat
    15661 taatgaggaa caaccggata tgattttagt acatggtgat actacaacga cttttgtagg
    15721 aagtttggca gcattttatc atcaaattcc ggtcggacat gtagaagctg gacttcgaac
    15781 acatcagaaa tactcaccat ttcctgaaga gttaaatcga gtcatggtaa gtaatattgc
    15841 tgaattgaat tttgcgccaa cagtaattgc agctaaaaat ttactttttg aaaacaaaga
    15901 caaagagcgt atctttatta ctggaaatac agttattgac gcattgtcaa caacagttca
    15961 aaatgatttt gtttcaacga ttattaataa acataaaggc aagaaagttg ttttactaac
    16021 agcgcatcgt cgtgaaaata ttggggaacc gatgcatcag atttttaaag cagtaagaga
    16081 tttggcagat gaatataaag atgttgtctt catttatcca atgcatcgta atccaaaggt
    16141 aagagcgatt gccgaaaaat atttatctgg gagaaatcgg attgaattaa ttgagccatt
    16201 agatgcgatt gagttccata attttacaaa tcaatcgtac ctcgtgctga cagattctgg
    16261 tggtattcaa gaggaggctc ctacatttgg aaaacctgtg ttggtattaa ggaatcatac
    16321 agagcgtccc gaaggcgttg aggcgggaac atcgagagta attggcacag attatgacaa
    16381 tattgttcga aatgtgaaac aattgattga ggatgatgaa gcgtatcaac gtatgagtca
    16441 agcgaataat ccatatggtg atggacaagc atcacgacgt atttgtgaag caatagaata
    16501 ttattttgga ttgcgcacag acaagccgga tgaattcgta cctttacgtc acaaataata
    16561 aaaaacccct aatcatgaag ttggtttaga caaccagcgg tgactagggg tttttaatat
    16621 atttattttt gatagtggta gccaatatca tatttgaata ctttatttga taatattgga
    16681 ctttgctgtc catcgtcatc actttttaaa cgtacatttt tatgagcttc tttaaataca
    16741 tcggaattca accaattatt aaagctatct tcagattccc aaatagttaa gattttaact
    16801 tcgtctgtat cctcggtatt taatgtttta gtgacaaaca tttgttggaa gccttcaata
    16861 gtttcaatac cttgtctatt gtaaaaacgt tcaatcgttt cttccgcact gcctttttgt
    16921 aattgtaatc tattttctgc cataaacatg ggcaatcact cctctatttt atgatttgat
    16981 ttgggtaatg tttttacaaa tgtaaagagt acagcggttt gtatgataac cattatgatt
    17041 aatcctacac ggactgcaag aacatccacc atataaattg aaaa
//
LOCUS       DAKTGS010000051        16751 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.051, whole genome shotgun sequence.
ACCESSION   DAKTGS010000051 DAKTGS010000000
VERSION     DAKTGS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.051"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..321
                     /locus_tag="O0286_001387"
     CDS             <1..321
                     /locus_tag="O0286_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0169770.1"
                     /translation="INIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALV
                     DEFRYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIK
                     MIRR"
     gene            329..1768
                     /locus_tag="O0286_001388"
     CDS             329..1768
                     /locus_tag="O0286_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0169771.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            1835..2770
                     /gene="trxB"
                     /locus_tag="O0286_001389"
     CDS             1835..2770
                     /gene="trxB"
                     /locus_tag="O0286_001389"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCY0169772.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            3650..4561
                     /gene="rapZ"
                     /locus_tag="O0286_001390"
     CDS             3650..4561
                     /gene="rapZ"
                     /locus_tag="O0286_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCY0169773.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            4558..5553
                     /locus_tag="O0286_001391"
     CDS             4558..5553
                     /locus_tag="O0286_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCY0169774.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            5663..6607
                     /gene="whiA"
                     /locus_tag="O0286_001392"
     CDS             5663..6607
                     /gene="whiA"
                     /locus_tag="O0286_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCY0169775.1"
                     /translation="MSFASEMKNELTRIDVEEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            7181..7768
                     /gene="clpP"
                     /locus_tag="O0286_001393"
     CDS             7181..7768
                     /gene="clpP"
                     /locus_tag="O0286_001393"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCY0169776.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(8011..8913)
                     /locus_tag="O0286_001394"
     CDS             complement(8011..8913)
                     /locus_tag="O0286_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCY0169777.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            9615..10244
                     /locus_tag="O0286_001395"
     CDS             9615..10244
                     /locus_tag="O0286_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCY0169778.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            10886..11899
                     /locus_tag="O0286_001396"
     CDS             10886..11899
                     /locus_tag="O0286_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCY0169779.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            11952..12962
                     /gene="gap"
                     /locus_tag="O0286_001397"
     CDS             11952..12962
                     /gene="gap"
                     /locus_tag="O0286_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0169780.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            13101..14291
                     /locus_tag="O0286_001398"
     CDS             13101..14291
                     /locus_tag="O0286_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCY0169781.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            14413..15174
                     /locus_tag="O0286_001399"
     CDS             14413..15174
                     /locus_tag="O0286_001399"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCY0169782.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            15177..16694
                     /locus_tag="O0286_001400"
     CDS             15177..16694
                     /locus_tag="O0286_001400"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCY0169783.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
BASE COUNT         6027 a         2436 c         3203 g         5085 t
ORIGIN      
        1 attaatatcg gtaatcaatc gtcgatagct tataaagtaa tgttagatat tttttaccca
       61 gaactgatta cgattggtag taacagtgtt attggttaca atgtaacaat tttgacgcat
      121 gaagcattag ttgatgaatt tcgttatgga ccagtgacga taggatctaa cactttgatt
      181 ggtgcaaatg ctaccatttt acccggtata acgattggtg acaatgtaaa agttgcagct
      241 ggtacggttg tttcaaaaga tataccggat aatggatttg catatggcaa ccctatgtat
      301 ataaaaatga ttaggaggtg acaattttat ggcgcaaaag aataataatg taattccaat
      361 gacttttgat gatgcatttt atcgtaaaat ggctaaacag aagtttaaac aaagagaata
      421 taaacgagct gctgaatact ttgaaaaagt gttagaattg tcacctgatg atatggaaat
      481 tcaaattgat tatgcacaat gtctagtgca acttggtatt gctaaaaaag cagaacattt
      541 attttatgac aatattattt ataataggca tctagaagat agcttttatg aattgagtca
      601 gctcaacatt gaagttaacg aaccaaacaa ggcattcttg tttggtatta attatgttat
      661 tgttagcgac gaccaagatt atagagatga attagatcaa atgtttgatg tgaaatatca
      721 aagtgaagaa caaattgaac ttgaagctca attgtttgta gttcaaatac tattccaata
      781 tcttttttct caaggtcgat taaaagatgc aaagaattat gtcttacatc aaccacaaga
      841 agttcaagat catcgtgtag tacgtaattt attggcaatg tgttatttat atctcggtga
      901 atatgatacg gctaaagcat tgtacgaagc actattacaa gaggatagta cagatatata
      961 tgcattatgc cattatactt tgctacttta taacactaag gaaaatgaac aatatcaaaa
     1021 atatttaaaa atattaaaca aagttgtacc tatgaatgac gatgaaagtt ttaaattagg
     1081 tattgtatta agttatttaa agcagtatcg tgcatcacaa caattgttgt accctttata
     1141 taaaaaaggg aaatttttat caattcaaat gtacaatgct ttagcatata attattatta
     1201 tttaggtgaa gaagacgaaa gtcattacta ctgggataaa ttgaagcaaa tttctaaagt
     1261 ggaaattgga catgcgcctt gggtaattga aaatagcaaa gaagtttttg accaacatat
     1321 tttgccatta cttcaaagtg atgacagtca ttatcgttta tatggtattt ttttattgga
     1381 tcaattaaat ggtaaagaaa ttgtgatgac ggaaagtatt tggcaggttt tggaaaatct
     1441 aaataattat gagaaattgt atttaacgta tttagttcaa ggtttaacgc tcaataaatt
     1501 agacttcatt catcgcggct tattaacgct ttaccataat gaattatttg taagtgaaaa
     1561 tgatgtaatg gttgcatgga ttaatcaagg tgaactcata attgctgaaa aagtagattt
     1621 aactgatgtt gagccatata tcggtgcgtt tatttatttg tattttaaaa atcaacctcg
     1681 aaacgttaca aagaagcaaa ttacaacatg gttaggcata acacaatata aactgaacaa
     1741 aatgattgaa tttctcttga gcatatagat ttatgaaaag ttagatttat tatataatgc
     1801 gcataatgat taataatgag gaggcgttaa taaaatgact gaaatagatt ttgatatagc
     1861 aattatcggt gcaggtccag ctggtatgac tgctgcagta tacgcatcac gtgctaattt
     1921 aaaaacagtt atgattgaaa gaggtattcc aggcggtcaa atggctaata cagaagaagt
     1981 agagaacttc cctggtttcg aaatgattac aggtccagat ttatctacaa aaatgtttga
     2041 acacgctaaa aagtttggtg cagtttatca atatggagat attaaatctg tagaagataa
     2101 aggcgaatat aaagtgatta actttggtaa taaagaatta acagcgaaag cggttattat
     2161 tgctacaggt gcagaataca agaaaattgg tgttccgggt gaacaagaac ttggtggacg
     2221 cggtgtaagt tattgtgcag tatgtgatgg tgcattcttt aaaaataaac gcctattcgt
     2281 tatcggtggt ggtgattcag cagtagaaga gggaacattc ttaactaaat ttgctgacaa
     2341 agtaacaatc gttcaccgtc gtgatgagtt acgtgcacag cgtattttac aagatagagc
     2401 attcaaaaat gataaaatcg actttatttg gagtcatact ttgaaatcaa ttaatgaaaa
     2461 agacggcaaa gtgggttctg tgacattaac gtctacaaaa gatggttcag aagaaacaca
     2521 cgaggctgat ggtgtattca tctatattgg tatgaaacca ttaacagcgc catttaaaga
     2581 cttaggtatt acaaatgatg ttggttatat tgtaacaaaa gatgatatga caacatcagt
     2641 accaggtatt tttgcagcag gagatgttcg cgacaaaggt ttacgccaaa ttgtcactgc
     2701 tactggcgat ggtagtattg cagcgcaaag tgcagcggaa tatattgaac atttaaacga
     2761 tcaagcttaa ttcgaagtcg aattaagatg ttgagctgta aattatttgg atatttattt
     2821 taatagtgtc atcacagcgt taaaataatg tcttactttt aaattaaagc aaattatata
     2881 gaaaactaga acttagtacg tatcatttgt gcgtttcaat gagttctagt ttttttatat
     2941 gttatattaa acttataact ttatgggagt gggacagaaa tgataaagag ccactaatga
     3001 tttattatgt agtggttctt aaacattagc cacagctaat gtgtacttaa aaataggaat
     3061 acatgagtaa aactcatgca taagaaatac taatttctat agaaaaagta ttactttatc
     3121 gttgtcccac cccaacttgc acattattgt aagctgactt tccgccagct tctgtgttgg
     3181 ggccccgcca acttgcacat tattgtaagc tgacttttcg tcagcttctg tgttggggcc
     3241 ccgccaactt gcattgtctg tagaaattgg gaatccaatt tctctatgtt ggggcccaca
     3301 ccccaactcg cattgcctgt agaatttctt ttcgaaattc tctgtgttgg ggcccacacc
     3361 ccaactcgca ttgcctgtag aatttctttt cgaaattctc tgtgttgggg cccctgacta
     3421 gagttgaaaa aagcttgttg caagcgcatt ttcattcagt caactactag caatataata
     3481 ttatagaccc taggacattg atttatgtcc caagctcctt ttaaatgatg tatattttta
     3541 gaaatttaat ctagacatag ttggaaataa atataaaaca tcgttgctta attttgtcat
     3601 agaacattta aattaacatc atgaaattcg ttttggcggt gaaaaaataa tggataataa
     3661 tgaaaaagaa aaaagtaaaa gtgaactatt agttgtaaca ggtttatctg gcgcaggtaa
     3721 atctttggtt attcaatgtt tagaagacat gggatatttt tgtgtagata atctaccacc
     3781 agtgttattg cctaaatttg tagagttgat ggaacaagga aatccatcct taagaaaagt
     3841 ggcaattgca attgatttaa gaggtaagga actatttaat tcattagttg cagtagtgga
     3901 taaagtcaaa agtgaaagtg acgtcatcat tgatgttatg tttttagaag caagtactga
     3961 aaaattaatt tcaagatata aggaaacgcg tcgtgcacat cctttgatgg aacaaggtaa
     4021 aagatcgtta atcaatgcaa ttaatgatga gcgagagcat ttgtctcaaa ttagaagtat
     4081 agctaatttt gttatagata ctacaaagtt atcacctaaa gaattaaaag aacgcattcg
     4141 tcgatactat gaagatgaag agtttgaaac ttttacaatt aatgtcacaa gtttcggttt
     4201 taaacatggg attcagatgg atgcagattt agtatttgat gtacgatttt taccaaatcc
     4261 atattatgta gtagatttaa gacctttaac aggattagat aaagacgttt ataattatgt
     4321 tatgaaatgg aaagagacgg agattttctt tgaaaaatta actgatttgt tagattttat
     4381 gatacccggg tataaaaaag aagggaaatc tcaattagta attgccatcg gttgtacggg
     4441 tggacaacat cgatctgtag cattagcaga acgactaggt aattatctaa atgaagtatt
     4501 tgaatataat gtttatgtgc atcataggga cgcacatatt gaaagtggcg agaaaaaatg
     4561 agacaaataa aagttgtact tatcggtggt ggcactggct tatcagttat ggctagggga
     4621 ttaagagaat tcccaattga tattacggcg attgtaacag ttgctgataa tggtgggagt
     4681 acagggaaaa tcagagatga aatggatata ccagcaccag gagacatcag aaatgtgatt
     4741 gcagctttaa gtgattctga gtcagtttta agccaacttt ttcagtatcg ctttgaagaa
     4801 aatcaaatta gcggtcactc attaggtaat ttattaatcg caggtatgac taatattacg
     4861 aatgatttcg gacatgccat taaagcatta agtaaaattt taaatattaa aggtagagtc
     4921 attccatcta caaatacaag tgtgcaatta aatgctgtta tggaagatgg agaaattgtt
     4981 tttggagaaa caaatattcc taaaaaacat aaaaaaattg atcgtgtgtt tttagaacct
     5041 aacgatgtgc aaccaatgga agaagcaatc gatgctttaa gggaagcaga tttaatcgtt
     5101 cttggaccag ggtcattata tacgagcgtt atttctaact tatgtgtgaa tggtatttca
     5161 gatgcgttaa ttcattctga tgcgcctaag ctatatgttt ctaatgtgat gacgcaacct
     5221 ggggaaacag atggttatag cgtgaaagat catatcgatg cgattcatag acaagctgga
     5281 caaccgttta ttgattatgt catttgtagt acacaaactt tcaatgctca agttttgaaa
     5341 aaatatgaag aaaaacattc taaaccagtt gaagttaata aggctgaact tgaaaaagaa
     5401 agcataaatg taaaaacatc ttcaaattta gttgaaattt ctgaaaatca tttagtaaga
     5461 cataatacta aagtgttatc gacaatgatt tatgacatag ctttagaatt aattagtact
     5521 attcctttcg taccaagtga taaacgtaaa taatatagaa cgtaatcata ttatgatatg
     5581 ataatagagc tgtgaaaaaa atgaaaatag acagtggttc taaggtgaat catgttttaa
     5641 ataagaaagg aatgactgta cgatgagctt tgcatcagaa atgaaaaatg aattaactag
     5701 aatagacgtc gaggaaatga atgcaaaagc agagctcagt gcactgattc gaatgaatgg
     5761 tgcacttagt ctttcaaatc aacaatttgt tataaatgtt caaacggaaa atgcaacaac
     5821 ggcaagacgt atttattcgt tgattaaacg tgtctttaat gtggaagttg aaatattagt
     5881 ccgtaaaaaa atgaaactta aaaaaaataa tatttatatt tgtcgtacaa agatgaaagc
     5941 gaaagaaatt cttgatgaat taggaatttt aaaagacggc atttttacgc atgaaattga
     6001 tcattcaatg attcaagatg acgaaatgag acgcagttac ttgagaggag cttttctggc
     6061 aggtggctca gtgaataacc ctgaaacatc ttcgtaccat ttggaaattt tttctcaaaa
     6121 tgagagtcat gcagaaggct taacgaaact aatgaatagt tatgagttga atgccaaaca
     6181 tttagagcga aaaaaaggaa gtattacgta tttaaaagaa gcggaaaaga tttcggattt
     6241 tcttagtttg ataggtggct atcaagcgtt attaaaattt gaagacgtac gtattgtaag
     6301 agatatgcgt aattctgtta accgactcgt taattgtgaa acggccaatc taaataaaac
     6361 agttagtgct gcgatgaaac aagttgagag cattaaattg attgataaag aaattggtat
     6421 tgaaaattta ccagacaggt tgagagagat tgctagaatt cgagtagaac atcaagaaat
     6481 ttcgttgaaa gagcttggag aaatggtatc aactggtcca atttcaaaat caggtgtaaa
     6541 tcaccgatta agaaaactga atgatttagc cgataagatt agaaatggtg aacaaataga
     6601 attataagta agaaggtgtt tttggtagtg aattatcaaa aacacttttt ttatgattaa
     6661 aaagtgttta atgaataata gtcgactaac tattaaattg agctggaaag tttaatgaag
     6721 gaaattcaat aaccaagtga tgcctaattt tctcattaaa tagcatcata tgtatcaatt
     6781 tggtcataag aaaatatgaa tgtgataatt aattgagtcc ctgaaagtcc ctgatcatag
     6841 acaaaaacaa aatcagcata aataagaatc ccgacattgc gggattctct gtattgaaaa
     6901 gtattgtatt ttattaaaac agcctccttg aagggaattg aacccctatc ttaagaaccg
     6961 gaatcttacg tgttatccat tacaccacaa ggagcaaata tgtaatcata tctctgtact
     7021 atgagaagta aaataagtac aatctaaaat agatattatc agtttacaaa ggaaagagaa
     7081 aagcgtcaaa caatgtaact atttaaagtc aaagtgtttg accaaatttg acttaatatg
     7141 taaaataatg agtaacagtt attacaagga ggaaatatag atgaatttaa ttcctacagt
     7201 tattgaaaca acaaaccgcg gtgaacgtgc atatgatata tactcacgtt tattaaaaga
     7261 ccgtattatt atgttaggtt cacaaattga tgacaacgta gcaaattcaa tcgtatcaca
     7321 gttattattc ttacaagcgc aagactcaga gaaagatatt tatttataca ttaattcacc
     7381 aggtggaagt gtaacagctg gttttgcgat ttatgataca attcaacaca ttaaacctga
     7441 tgttcaaaca atttgtatcg gtatggctgc atcaatggga tcattcttat tagcagctgg
     7501 tgcaaaaggt aaacgtttcg cgttaccaaa tgcagaagta atgattcacc aaccattagg
     7561 tggtgctcaa ggacaagcaa ctgaaatcga aattgctgca aatcacattt taaaaacacg
     7621 tgaaaaatta aaccgcattt tatcagagcg tactggtcaa agtattgaaa aaatacaaaa
     7681 agacacagat cgtgataact tcttaactgc agaagaagct aaagaatatg gcttaattga
     7741 tgaagtgatg gtacctgaaa caaaataatt caaagtaaag agtagactaa gctgtctgct
     7801 ctttttgtat gagtaaacca aggtgtcaat aatttgttta ctatactttg agcggaaata
     7861 tgattgaatg aagctagttg aaccgtaact atatgaaatg ttcccttcaa agtagacatt
     7921 gaaaggaaca tttcaatcct ttgtttgtaa gtcgctctag acattacatt tagtacatat
     7981 gttgtttcta atgctcatta atggtattga ttattcttta attaaatctt caagtgccat
     8041 ttttaaatta ctatatttaa attggaatcc caatgcttga attttattag gtaatacttt
     8101 ttgagtatcc aatactactg ttgacatttg accaagtatg agacgcattg caagacttgg
     8161 tgcccaagtt tcatgaggct tatgcatagc tcttgctaaa gtgtagccaa ataaattttg
     8221 acgttcaggt ataggtgcag ttaaattaaa cggaccacta gctgactcgt tatttattaa
     8281 aaataaaata gcttgaatta aatcattgat atgaatccat gaataccatt gttgaccaga
     8341 acctaattta ccaccaatgt aatattcgta tggtagtttc attgtttgta acgcaccgcc
     8401 ttcattcgat aaaattatac cgaaacgacc gatgacaact cgtgtaccta attgttcaaa
     8461 ttgttgtgcg aaacgttccc attgatacac aatatctgat aagaaatcaa aaggtaaagt
     8521 tttataaact tctgtgtaac tcataaataa atcaggagga tagtaaccag tggcactagc
     8581 attaaataaa gctttaggtg ctttattacg tgatttaaac aattcatata aagcttgcgt
     8641 agattgaatt ctacttagca ttagcgtttg tttatattcc ggtgtccatc gtttattcaa
     8701 tgtagcacct gctaagttga tgaccacatc gatattttga ggaactttgt gttcccaccc
     8761 agatttagcc cagttgacat atgaaatttt cttatcattt gaaatttggt cgtgtcgcgt
     8821 taatatcgtg atatgtgaat ctgatttttt aatttcatta actaattgag atccaaccat
     8881 accagtccca ccagtaatta agtattgttt cattatcatt caccccatgt aattttgtat
     8941 ttagttacta tttaataatc attattgttg ttcaaaggtt atacattatt tagacaataa
     9001 tatgtcaata actttttttg aattgtattt taataaaaat gatataagtt ataataaagg
     9061 tgtaccttcg ataacgaata aacatctctt aaaagtatgt gtaaaacgct gcatgataca
     9121 aacgaaggta aaaatttgac tccctttagt agtggacccg tacgttaatc gtgcgggtcg
     9181 tttttattta tttcttattc ccattataca tcaatttaaa gcattaattt tttaaacaaa
     9241 tttaagaata catagtaata taacaatcta aacataaaaa cttttaacac aacacttaaa
     9301 ccaatgcttt aattttcaat acgtagctat aatttgttgt aaaatcaaaa aggttaaaat
     9361 gttaattttc aaaaaaggct caaaatatgt ttgatttagt tattaaatgt taagatatat
     9421 aagactacta tttctttgta aaaatgaatc cgatttacga gtgagtaata gtgaaggcag
     9481 ttttaagttg aagaaggcaa aaagagtaaa tgtttattaa tatttgtaga aactaggtaa
     9541 gcaaattagt tgtgaaaatg ttaatggttg cgtgataatt tctatattta aattagtttg
     9601 aagtgaggga gagtatgtcg aatcaaaatt acgactacaa taaaaatgaa gatggaagta
     9661 agaagaaaat gagtacaaca gcgaaagtag ttagcattgc gacggtattg ctattactcg
     9721 gaggattagt atttgcaatt tttgcatatg tagatcattc gaataaagct aaagaacgta
     9781 tgttgaacga acaaaagcag gaacaaaaag aaaagcgtca aaaagaaaat gcagaaaaag
     9841 agagaaagaa aaagcaacaa gaggaaaaag agcagaatga gctagattca caagcaaacc
     9901 aatatcagca attgccacag cagaatcaat atcaatatgt gccacctcag caacaagcac
     9961 ctacaaagca acgtcctgct aaagaagaga atgatgataa agcatcaaag gatgagtcga
    10021 aagataagga tgacaaagca tctcaagata aatcagatga taatcagaag aaaactgatg
    10081 ataataaaca accagctcag cctaaaccac agccgcaaca accaacacca aagccaaata
    10141 ataatcaaca aaacaatcaa tcaaatcagc aagcaaaacc acaagcacca caacaaaata
    10201 gccaatcaac aacaaataaa caaaataatg ctaatgataa gtagtattta gtcaaacaaa
    10261 aatgaaccag tatgacagac aacacaatta attaggttgt ctcgaatatt ggttcttatt
    10321 tttataattg ttaattaggg gagagatgat acttaaatag ttagttattt attttacgga
    10381 tagtgaaatt tattttgagt gaggtgggac agaaatgata ttttcgcaaa atttatttcg
    10441 tcgtcccacc ccaactcgca ttgcctgtag aatttcattt cgaaattctc tatgttgggg
    10501 cccctgactt taattgaaaa aagcttgtta caagtgcatt ttcgttcggt taactactac
    10561 taatgtgact ttttggattc tagagcattg atttatgtcc tagtctcaaa taataagcaa
    10621 atgattatcg agtcagtata agggtataca tttgactaca gcgaataaaa taaaacgttt
    10681 tgttgaataa caaatcgaaa atatattgca agcgctttat caaattattt agaaaattta
    10741 agttttatgc ttgcaatttt tgaaatagaa tagtactatt gcaagtgtaa agaggttaat
    10801 ttttgtccca cgcgggactt aaaaaggcaa ccactggttg tgacatatcc ttatttacat
    10861 ttataaatat aaggaggagg tagtagtgaa agacttattg caagcacagc aaaagcttat
    10921 accggatctc atagataaaa tgtataaacg tttttctatt cttactacta tctcaaaaaa
    10981 tcagcctgtc ggacgtcgaa gtttaagcga acatatggat atgactgaac gtgtactgcg
    11041 ttctgaaaca gatatgctta agaaacaaga tttgataaaa gttaagccta ccggaatgga
    11101 aattacagct gaaggtgagc aactgatttc gcaattgaaa ggttactttg atatctatgc
    11161 agatgataat cgtctgtcag aaggtattaa gaataaattt caaattaagg aagttcatgt
    11221 tgttcctggt gatgctgata atagtcaatc tgttaaaaca gaattaggta gacaagcagg
    11281 tcaattactt gaaggcatat tacaagaaga cgcgatagtt gctgtaactg gcggatccac
    11341 gatggcatgt gttagtgaag caattcattt attaccatat aatgtattct tcgtaccagc
    11401 cagaggtgga ctaggcgaaa atgttgtctt tcaggcaaac acaattgcag ccagtatggc
    11461 acaacaagct ggcggttatt atacgacgat gtatgtacct gataatgtca gtgaaacaac
    11521 atataataca ttgttgttag agccatcagt cataaacact ttagacaaaa ttaaacaagc
    11581 aaacgttata ttacacggca ttggtgatgc gctgaagatg gcgcatcgac gtcaatcacc
    11641 tgaaaaggtc attgaacaac ttcaacatca tcaagctgtc ggagaggcat ttggttatta
    11701 ttttgataca caaggtcaaa ttgtccataa ggttaaaaca attggacttc aattagaaga
    11761 ccttgaatca aaagacttta tttttgcagt tgcaggaggc aaatcgaaag gtgaagcaat
    11821 taaagcatac ttgacgattg cacccaagaa tacagtgtta atcactgatg aagccgcagc
    11881 aaagataata cttgaataag agataaaaag tttaatactt tttaaatatc attttaaagg
    11941 aggccattat aatggcagta aaagtagcaa ttaatggttt tggtagaatt ggtcgtttag
    12001 cattcagaag aattcaagaa gtagaaggtc ttgaagttgt agcagtaaac gacttaacag
    12061 atgacgacat gttagcgcat ttattaaaat atgacactat gcaaggtcgt ttcacaggtg
    12121 aagtagaggt agttgatggt ggtttccgcg taaatggtaa agaagttaaa tcattcagtg
    12181 aaccagatgc aagcaaatta ccttggaaag acttaaatat cgatgtagta ttagaatgta
    12241 ctggtttcta cactgataaa gataaagcac aagctcatat tgaagcaggc gctaaaaaag
    12301 tattaatctc agcaccagct actggtgact taaaaacaat cgtattcaac actaaccacc
    12361 aagagttaga cggttctgaa acagttgttt caggtgcttc atgtactaca aactcattag
    12421 caccagttgc taaagtttta aacgatgact ttggtttagt tgaaggttta atgactacaa
    12481 ttcacgctta cacaggtgat caaaatacac aagacgcacc tcacagaaaa ggtgacaaac
    12541 gtcgtgctcg tgcagcggca gaaaacatca tccctaactc aacaggtgct gctaaagcta
    12601 tcggtaaagt tattcctgaa atcgatggta aattagatgg tggtgcacaa cgtgttcctg
    12661 tagctacagg ttcattaact gaattaacag tagtattaga aaaacaagac gtaacagttg
    12721 aacaagttaa cgaagctatg aaaaatgctt caaacgaatc attcggttac actgaagacg
    12781 aaatcgtttc ttcagacgtt gtaggtatga cttacggttc attattcgac gctacacaaa
    12841 ctcgtgtaat gtcagttggc gaccgtcaat tagttaaagt tgcagcttgg tatgataacg
    12901 aaatgtcata tactgcacaa ttagttcgta cattagcata cttagctgaa ctttctaaat
    12961 aattttagta tagtttttat tcaaatacgc tagtgctcag aactatttag cattaattaa
    13021 agcttatgag taagcgggga gcacaaacgc ttctccgctt atttttatat aaaatttcct
    13081 aattacaagg aggaaacacc atggctaaaa aaattgtttc tgatttagat cttaaaggta
    13141 aaacagtcct agtacgtgct gattttaacg tacctttaaa agacggtgaa attactaatg
    13201 acaaccgtat cgttcaagct ttacctacaa ttcaatacat catcgaacaa ggtggtaaaa
    13261 tcgtactatt ttcacattta ggtaaagtga aagaagaaag tgataaagca aaattaactt
    13321 tacgtccagt tgctgaagac ttatctaaga aattagataa agaagttgtt ttcgtaccag
    13381 aaacacgcgg cgaaaaactt gaagctgcta ttaaagacct taaagaaggc gacgtattat
    13441 tagttgaaaa tacacgttat gaagatttag acggtaaaaa agaatctaaa aatgatccag
    13501 aattaggtaa atactgggca tctttaggtg atgtgtttgt aaatgatgct tttggtactg
    13561 cgcatcgtga gcatgcatct aatgttggta tttctacaca tttagaaact gcagctggat
    13621 tcttaatgga taaagaaatt aagtttattg gcggcgtagt taacgatcca cataaaccag
    13681 ttgttgctat tttaggtgga gcaaaagtat ctgacaaaat taatgtcatc aaaaacttag
    13741 ttaacatagc tgataaaatt atcatcggcg gaggtatggc ttatactttc ttaaaagcgc
    13801 aaggtaaaga aattggtatt tcattattag aagaagataa aatcgacttc gcaaaagatt
    13861 tattagaaaa acatggtgat aaaattgtat taccagtaga cactaaagtt gctaaagaat
    13921 tttctaatga tgccaaaatc actgtagtac catctgattc aattccagca gaccaagaag
    13981 gtatggatat tggaccaaac actgtaaaat tatttgcaga tgaattagaa ggtgcgcaca
    14041 ctgttgtatg gaatggacct atgggtgtat tcgagttcag taactttgca caaggtacaa
    14101 ttggtgtatg taaagcaatt gcaaacctta aagatgcaat tacgattatc ggtggcggtg
    14161 attcagctgc agcagcaatc tctttaggtt ttgaaaatga cttcactcat atttcaactg
    14221 gtggcggcgc gtcattagag tacctagaag gtaaagaatt gcctggtatc aaagcaatca
    14281 ataataaata ataaagtgat agtttaaagt gatgtggcat gtttgtttaa cattgttacg
    14341 ggaaaacagt cacaagatga catcgtgttt catcactttt caaaaatatt tacaaaacaa
    14401 ggagtgtctt taatgagaac accaattata gctggtaact ggaaaatgaa caaaacagta
    14461 caagaagcaa aagacttcgt caatgcatta ccaacattac cagattcaaa agaagtagaa
    14521 tcagtaattt gtgcaccagc aattcaatta gatgcattaa ctactgcagt taaagaagga
    14581 aaagcacaag gtttagaaat cggtgctcaa aatacgtatt tcgaagataa tggtgcgttc
    14641 acaggtgaaa cgtctccagt tgcattagca gatttaggcg ttaaatacgt tgttatcggt
    14701 cattctgaac gtcgtgaatt attccacgaa acagatgaag aaattaacaa aaaagcgcac
    14761 gctattttca aacatggaat gactccaatt atatgtgttg gtgaaacaga cgaagagcgt
    14821 gaaagtggta aagctaacga tgttgtaggt gagcaagtta agaaagctgt tgcaggttta
    14881 tctgaagatc aacttaaatc agttgtaatt gcttatgaac caatctgggc aatcggaact
    14941 ggtaaatcat caacatctga agatgcaaat gaaatgtgtg catttgtacg tcaaactatt
    15001 gctgacttat caagcaaaga agtatcagaa gcaactcgta ttcaatatgg tggtagtgtt
    15061 aaacctaaca acattaaaga atacatggca caaactgata ttgatggggc attagtaggt
    15121 ggcgcatcac ttaaagttga agatttcgta caattgttag aaggtgcaaa ataatcatgg
    15181 ctaagaaacc aactgcgtta attattttag atggttttgc gaaccgcgaa agcgaacatg
    15241 gtaatgcggt aaaattagca aacaagccta attttgatcg ttattacaac aaatatccaa
    15301 cgactcaaat cgaagcgagt ggcttagatg ttggactacc tgaaggacaa atgggtaact
    15361 cagaagttgg tcatatgaat atcggtgcag gacgtatcgt ttatcaaagt ttaactcgaa
    15421 tcaataaatc aattgaagac ggtgatttct ttgaaaatga tgttttaaat aatgcaattg
    15481 cacacgtgaa ttcacatgat tcagcgttac acatctttgg tttattgtct gacggtggtg
    15541 tacacagtca ttacaaacat ttatttgctt tgttagaact tgctaaaaaa caaggtgttg
    15601 aaaaagttta cgtacacgca tttttagatg gccgtgacgt agatcaaaaa tccgctttga
    15661 aatacatcga agagactgaa gctaaattca atgaattagg cattggtcaa tttgcatctg
    15721 tgtctggtcg ttattatgca atggatcgtg acaaacgttg ggaacgtgaa gaaaaagctt
    15781 acaatgctat tcgtaatttt gatgccccaa cttatgcaac tgccaaagaa ggtgtagaag
    15841 caagctataa tgagggctta actgacgaat tcgtagtacc attcatcgtt gagaatcaaa
    15901 atgacggtgt taatgatgga gatgcagtga tcttctataa tttccgacct gatagagcag
    15961 cgcaattatc ggaaattttt gcgaacagag cattcgaagg ctttaaagtt gaacaagtta
    16021 aagacttatt ctatgcaaca ttcactaagt ataatgacaa tatcgatgcg gctatcgtct
    16081 tcgaaaaagt tgatttaaat aatacaattg gtgaaattgc acaaaataac aatttaactc
    16141 aattacgtat tgcagaaact gaaaaatacc ctcacgttac ttactttatg agtggtggac
    16201 gtaacgagga atttaaaggt gaacgccgtc gtttaattga ttcacctaaa gttgcaacgt
    16261 atgacttgaa accagaaatg agtgcttatg aagttaaaga tgcattatta gaagagttaa
    16321 ataaaggtga cttggactta attattttaa actttgctaa ccctgatatg gttggacata
    16381 gtggtatgct tgagccgaca atcaaagcaa tcgaagcggt tgatgaatgt ttaggagaag
    16441 tggttgataa gattttagac atggacggtt atgcaattat tactgctgac catggtaact
    16501 ctgatcaagt attgacggat gatgatcaac caatgactac gcatacaacg aacccagtac
    16561 cagtgattgt aacaaaagaa ggcgttacac ttagagaaac tggtcgctta ggtgacttag
    16621 cacctacatt attagattta ttaaatgtag aacaacctga agatatgaca ggtgaatctt
    16681 taattaaaca ctaatattgt aaaagatgtt aagtaaacgc ttaatgacac ttattttttg
    16741 aaaataatag t
//
LOCUS       DAKTGS010000052        16606 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.052, whole genome shotgun sequence.
ACCESSION   DAKTGS010000052 DAKTGS010000000
VERSION     DAKTGS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.052"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..654)
                     /locus_tag="O0286_001401"
     CDS             complement(<1..654)
                     /locus_tag="O0286_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169784.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     "
     gene            complement(816..1322)
                     /locus_tag="O0286_001402"
     CDS             complement(816..1322)
                     /locus_tag="O0286_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169785.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(1458..2258)
                     /locus_tag="O0286_001403"
     CDS             complement(1458..2258)
                     /locus_tag="O0286_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0169786.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            complement(2242..2961)
                     /locus_tag="O0286_001404"
     CDS             complement(2242..2961)
                     /locus_tag="O0286_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0169787.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(3016..3966)
                     /locus_tag="O0286_001405"
     CDS             complement(3016..3966)
                     /locus_tag="O0286_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0169788.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(4114..5001)
                     /locus_tag="O0286_001406"
     CDS             complement(4114..5001)
                     /locus_tag="O0286_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0169789.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(5380..6015)
                     /locus_tag="O0286_001407"
     CDS             complement(5380..6015)
                     /locus_tag="O0286_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCY0169790.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(6403..8064)
                     /locus_tag="O0286_001408"
     CDS             complement(6403..8064)
                     /locus_tag="O0286_001408"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCY0169791.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(8061..8477)
                     /locus_tag="O0286_001409"
     CDS             complement(8061..8477)
                     /locus_tag="O0286_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCY0169792.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(8570..8677)
                     /locus_tag="O0286_001410"
     CDS             complement(8570..8677)
                     /locus_tag="O0286_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169793.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(8752..9762)
                     /gene="adhP"
                     /locus_tag="O0286_001411"
     CDS             complement(8752..9762)
                     /gene="adhP"
                     /locus_tag="O0286_001411"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCY0169794.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            complement(10261..10782)
                     /locus_tag="O0286_001412"
     CDS             complement(10261..10782)
                     /locus_tag="O0286_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCY0169795.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(10810..12105)
                     /locus_tag="O0286_001413"
     CDS             complement(10810..12105)
                     /locus_tag="O0286_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0169796.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(12145..12621)
                     /locus_tag="O0286_001414"
     CDS             complement(12145..12621)
                     /locus_tag="O0286_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0169797.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(12790..13326)
                     /locus_tag="O0286_001415"
     CDS             complement(12790..13326)
                     /locus_tag="O0286_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCY0169798.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(13537..14475)
                     /locus_tag="O0286_001416"
     CDS             complement(13537..14475)
                     /locus_tag="O0286_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0169799.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(14745..15371)
                     /locus_tag="O0286_001417"
     CDS             complement(14745..15371)
                     /locus_tag="O0286_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0169800.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIFLIPTLKNVCFTLFGYILFGGLT
                     MLFLDALLSMSYQNIIVYFVWIAVIMGFFLVNIALIIDKNIHVILKNQ"
     gene            complement(15395..15640)
                     /locus_tag="O0286_001418"
     CDS             complement(15395..15640)
                     /locus_tag="O0286_001418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169801.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            complement(15606..16241)
                     /locus_tag="O0286_001419"
     CDS             complement(15606..16241)
                     /locus_tag="O0286_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0169802.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            complement(16278..>16606)
                     /locus_tag="O0286_001420"
     CDS             complement(16278..>16606)
                     /locus_tag="O0286_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0169803.1"
                     /translation="IIILGIFLFFLYLNQKLKLQIYNENKNKSNKIIIFPTLKSLCLS
                     IVLYIYLGGGSFFTIYMLLTIEVQNIILFITLFVIFLFFLFLNMCSLYDNKVHVLFKS
                     NGIEKF"
BASE COUNT         5381 a         3063 c         2123 g         6039 t
ORIGIN      
        1 ccatataatt aatgaaaaaa tctgggctga ttgtggatta tgaatcattg catcgacggt
       61 tttttgacaa aataataacc cgattcccga aaactcaagt cgcttataaa atatgccggc
      121 aattactaaa ataataccaa tgaaaaagaa aaattgatgt ccaaaatttg taattggtat
      181 aacgccccaa gtaatattaa atgcttgtaa tataattaat aacaaggata tgatagcgat
      241 aattgtaccc ataatcattt ctattttatt actacgcaag taattctttt gagacaccct
      301 taggtataca acaatagtca ttggaaaaat cactaaacaa taaaatagac tcattccagt
      361 aatagcttga aactgaacat actgtttatc atataaatag gatatgagaa tgaagtttat
      421 aataaagaaa ataactggat tcatttgtaa agcaaacaaa ttcctttgaa tcattaatat
      481 gatttgtgct ggagattttc ttttatattc aatataatct tgatctttct tttcagaatt
      541 ttttatatca gtaattactt tctctttaat atcatcaatc aaatgcggtg ataaaccttt
      601 atgattaaag taatcttcta tattttctag taaaatttta tcgttaatac gcattggtag
      661 tactccttta aaatgaaaca tctttattta ttgtattggt ttaatttccc cttcgcaaat
      721 cataaaaatt aatattcact gtattatgcc atgtaataaa ataaccgaca agtattgcga
      781 tttaacttgt cggttaagca cgattaattt aattattatt tttccaaatc aatacgatat
      841 aattttatgt ttttatcttt tggtgctgag gcaaattgat aagtcgtttt gcctttttca
      901 atatatccaa aattaccagc aactttgcta tattccgtat gtgttactgc tttgccaaca
      961 taatttttaa cagcttgata tgttggacca ttagcatcat tatacatcat tgaaatatga
     1021 gttactttac cattgttttt cttaccatca gtagttacta ataacatacc ttttttagta
     1081 tggaattcgt aataatgttc agttccatct tcattgtaag aatacatcgg atttttgtac
     1141 ttagttaaaa catctttaat agattcgcca attttcacat tttctaatga ctgatcaccc
     1201 ttaattaatt gtttaacagt atcaatagag ttgccacttg ctgcttcggc actagtgttc
     1261 actaagccta ctcccattac aacagaacat gcaattatac ttgccgttag tagctttttc
     1321 ataagcactc tctcctttta tttatatcgt cttgtgctca caaccattat acaacaatca
     1381 tttctaaaac aacagtcata ttacaattat attacaaata ataattacta cttttatatt
     1441 tcacttatca ctaaaaatta aacatgcttt tcatcgtgat gtttgttgat aaatgcagct
     1501 gtatcttcga cgaatctttc ttgctcttca acaaatggga atgcacttga ttcttgatat
     1561 acttcaaagt ccgcattttg gattaaatca gctacttctt tagcttctaa tcttgttgtt
     1621 ctttcaccga attctcctgc aataattttt gtcggtacta cgacatttct ataagtttga
     1681 gaaatatcag cgtttttgaa tacctcttta actgcttgaa tttcttcttt agttgatatt
     1741 tcatttgtat ctacgacatg tttgaggaat cgattcattt ttctcggacg ataatactta
     1801 cgcttattta aaaatttatc ttgtttttca ggatcccagt ttcgaataat atgggcatat
     1861 tttctaaata aacgttcttc cggtaattca ccttcaatag atgttggatt taccaacgta
     1921 agtgatgatg taaattcagg ataacgtact gaaatatccg caccaatgat tccacccatt
     1981 tcatggcata caaatgcaac ttcttcaata tataaatatt taagtaattc aacaatgtca
     2041 tcagaaaaat cttttatttc aatgtgacga ggtttatcag aatagccatg tccacgtaaa
     2101 tcaattaaca caacttgaaa tgattttgct aattctgctg ctaatttatt aaacacagaa
     2161 taattatcaa gtacagtatg aatcaatacg ataggatagc cttcacctaa tgtactgtaa
     2221 tgtatcgatg ttccatcttt tctagtaaat agatccataa ttttctcctt tattatcatt
     2281 ttttattggt aaaatggtta ataattcttc cactgttgta attgtaaaat caacttcttc
     2341 ttcgagtggt tcaatttcag catcttcttg tttataccat acactaacca tgcccatagc
     2401 tcgtgctgga gctacatcat ttaacgcatc atcgccaaca tacataattt gctcaggtaa
     2461 tacccctagt tgatcaatca tatcttcaaa aattttagga tgtggtttac gaaaaccaac
     2521 tgtttctgat gttgataaat aattaataac atgcatcaaa ccaagtgaat gtaatcgaaa
     2581 ttgtttaatc ttagatttac catttgcgat aacaccaatt tgaaagcctt tttgcgatag
     2641 cttttctagt gtatacaaag tatcgtaata aggaaataca taacgataaa aatgcatttc
     2701 aaaatcatta aataagtctt tccaagttaa cctatctaca tggaaacgtt taatgatttc
     2761 tttatataaa tcaggtttat cattatcttc atcgtcatct agctcaataa atgctttttt
     2821 aaaatctgcc agttgaacat gaattaagta gtcatgaaat cgttcatatt gctcttcgat
     2881 aaatttctct cgagatttaa ctctgtccaa caacgtacct tctaaatcaa atacaactgc
     2941 tttaatttga ttcaaatcca tcgcttatgc cctctttatg tttatagtta tttttatttt
     3001 agcaaatgtc gtgtgctagt gtaaccgttt cgactgagat attagcatgt aaaataacac
     3061 tggtatacca attaatgcaa tgataatact tgccggtatt tgtactggtg acaatatatg
     3121 gctaccaata aaatctgcca taaccattat ttgtcctcca gttacaatat ttaaagccaa
     3181 ttgtcttcct aatgattgtt tccactgcca ttttctaatg agttgcggta tcaccatacc
     3241 aatgaatcca atgatgccaa catatgcgac atttatcgcc gttatcatag aagctataca
     3301 taacgcgata tatgtaatca attgaacatt taagcctagt gactgacttt ttagttctcc
     3361 taattgcaat aactttagtt gattaagaat gataaatata ccaaacaatg caatgataaa
     3421 tgtgattgct attatagata catttgagta ttctgcatca ccaaaaccac caaacagata
     3481 attggcaatt gtatttaatt tgcgaggttt taataaaatc aaaaaatata gaagtgaatt
     3541 gaataacgca ccaatcatta aaccacttaa tattaagatt ctaactggat agccttggct
     3601 caataccgac gtaatgacta atacagttat taatgttatc aaactaaatg ttattgaaaa
     3661 tacaggaatc cataacgttg ttaaacctaa aaataatgct aatcctgaac caaatgtagc
     3721 gccgcttgcc aatcctaatg taaagctatc tgccaatgca ttatttaaaa ctgtttgaaa
     3781 tataaggcct gcaacagtta atatcatacc tgtcaacaat gcttctaata atctcggtaa
     3841 tctaacattg attaaaatag actggttaaa tacatcattg atatcgccca actgccaaaa
     3901 tagatatatg ctagttgtta taatcaatat gactatccag ctcaataata ctttattaaa
     3961 tgtcatagca ttctcttttc tttcatcgat aattacattc attatagcaa tcacatactc
     4021 gcgttgctat cattcactaa atatatttta aaaacccctt atctcaaaca tgtatttttt
     4081 acatgtagca ataaggggca taattagaat ggtttatttt ctataaattg catctcttaa
     4141 ttcttttaat ccttcatcaa tacgtggacc tggtcttgat acttcatcac catttacaac
     4201 ttcaatacgt gtattcttga cagcattaat tttattgaat ccacctcttt ttttgatgat
     4261 atccatataa tctgatcttg tcttagcttc cgtcgaaatt aatatatctg ggttcttttt
     4321 aataatactt tccttcgtta cagggttcca accattaatg tcactataca cattttgggc
     4381 ttctaatttt tctaacatat cattaaaaaa tgtatgcttc cctgctgtat atatttcagg
     4441 ctttgatgaa acctcaataa atacttttga ttttttatga tgagcaggaa ttgaatctat
     4501 gactttatcg atattatctt tagtttcctc aacaagttgt tcagcctgct tatcatgatg
     4561 cgttaatttc ccaatttgct taaatgtgtt gtaagtttca tcaattgatt gtgcatcttt
     4621 aacatacact actttgatgc cttgtttctc taatgatgac aatactttat tagcagttgc
     4681 cttttgcgac tcatgcgcaa gaattagatc tggctttgcc tttaaaagtt cctctttatt
     4741 tagattcaaa gcatcaaatt gtttcttacc ctttttcaca tcttttggat aatcatcaac
     4801 cgttgaaaca ccaactatgt atttacctaa tcctaattca tataaaattt cagtattact
     4861 aggcattaac gaaacaattc tatggtacgg cgatttagac gagccattat cattgctacc
     4921 ttgtttatca ttatctttca taccacagcc actcaggaca agcataaaaa tcaaaataaa
     4981 agcaattaac gatttcttca cactatcaca tctccttttt aatcccactt ttcgctttag
     5041 cgattagagg gatttatgca gttgtgcggt agcatccaac tgcgttcctc ttcatcttta
     5101 gcgattagag gcttttatgc agttgtgcct tagcatccaa ctgcgttcct cttcaccttt
     5161 agtgattaga ggcttttatg cagttgctgc acaaacaact gtattccttt actccccttg
     5221 agctttagcg attaggggct cttatgcagt tgctgcacaa acaaacaact gtttttttct
     5281 ttatatgctt ctatttatta tataagtcca catactaaaa gaaaatagta aattattttt
     5341 tatatttcat ataaactcat ctatctaact tcacagcgtt tactttttaa agtaaggttc
     5401 taaaaaatca taattcttta tatctttgtc tctaaagtaa tgtttaccaa atacatctaa
     5461 cagagcatga aattcattag catcttgatt tgtaaaatga tttggtaatg tgactacttt
     5521 ttttaattga tcataatttt tagtgttttc atatcctaat ttatcatata tttttcttgt
     5581 atagctatct ggaataaatt caatacgtcc gaatatataa acaagtaaga catctgctgt
     5641 ttcacttcca atacctttca aagataataa ttcttttctt aatccacctt tatatcgctc
     5701 attaatctct tgataattga aatgatgtcg tgctaaccat gttaataatg ttttaatcgt
     5761 cagtgattta cttttataaa agccacttga atgtatcaat gattgtaacg tttcaatagg
     5821 tagttctaat atatgatttg gattaaaatg cgtatgttct ttaatctgat tcaatgcaat
     5881 ttctgcattt cgccatctag tattttgaac taaaattgca cctaacatca tttcaatgtc
     5941 attatcagca ggccaccaaa attgtggtcc gagatgttca tataaaactt tatataattc
     6001 atcagttcct aacatttaca ccctccattt tagagggagt gggacagaaa tgatattatc
     6061 gtaaaattta tttcgtcgtc ccaccccaac ttgcattgtc tgtagaaatt gggaatccaa
     6121 tttctctttg ttggggcccc gccggcaagg ttgactagaa ttgaaaaaag cttgttacaa
     6181 gcgaattttc gttcagtcaa ctactgccat tataactttg tagagcatag aacattgatt
     6241 tatgtctcag gctctcttat gcagttgtgc agagcatcca actgtgttcc tcttaacctt
     6301 attcacatac taagcattcc ccaatatgtc acatacctaa aaaaacactc caacacaaag
     6361 tatctttcga gaaactctac gttggagtgt atataaatgt tcttacattg attcaggtgc
     6421 gcttacaccg accattgcca atgcattttt caatgtaatt ctgaccgctt caatcatagc
     6481 aacatgtgct tttgtttttt caatatcatc tgttaacact ttttcagcat tatagaattt
     6541 atggaaatga gaagctaaat cttgaatata attagtaatt ctatgtgccg atctatgctc
     6601 agcagcactt tcaattgtag gttcgaaatc agctactttt ttcaacaatt caatcgcttt
     6661 ttcattagta atcgttgtaa aatcattcgc agcagtcact tcaatacctt gctctttcgc
     6721 ttgttttaaa attgaacaaa tacgcgcatg tgcatattga gcatagtaaa ctggattgtc
     6781 ttgagattgc tctttcgcta attccatatc aaaatcaaag tgactatcag gactacgcat
     6841 agttaagaaa taacgtgcag cgtcaacgcc aacttcgtcc ataatttctc ttaatgtaat
     6901 cgcattacca gtacgtttac tcatcttcac ttctttacca ttttccatta aacgaaccat
     6961 ttgcatgatt tgaatttcta aacgattact atctacacca aacgtttcaa gagatgcttt
     7021 caaacgatta atataaccat gatgatcagc accaaataaa tcgattaaaa tgtcattacc
     7081 acgttttact ttatcgaagt ggtacgcaat atctggtaag aaatacgtat atgtaccgtc
     7141 atttttaatt aatactctgt ctttgtcgtc tttaaaatca gttgtacgta accatgtagc
     7201 gccatcagct tcatacgtat aacctaattc tttcattttt gctaaaactt caagaatttc
     7261 tcctttttca tataaagatg tttcactaaa ccaattatca aaatgcgtat tgaactctgc
     7321 taaatcattt ttcaatttag ccatttcgta ttctacgcct aattttctaa attctttcaa
     7381 acgtgcttct tcagaataat ctttaatttc agggtgtttc tctgctaaat ctttacctat
     7441 ttcaataata tcttttccat tatagccatc ttctggcatt gaataactat tgtcacctaa
     7501 agcttcaaag aaacgtgttt caatcgaacg cgctaagtta gtaatttgat taccagcatc
     7561 attaatataa tattcacgtg ttacattata gccagctgca gttaaaatat tagctaaagc
     7621 atcaccaact gctgcatttc tagcatgacc aatatgtaaa tctcctgtag ggttagctga
     7681 aacatactca agcaatacat tttgaccttt tgattcattt acatgtccaa attgatcacc
     7741 tttttcaatt gcttcaggaa taattgctgt taaatactga ttatctaagt aaaaattaat
     7801 gaatcctgga ccagcaatgt caatttgttt tacatgtgct ttttcagtat ctaagttatc
     7861 aacaatcgct tgagcaattt cacgaggatt acgctttgca atcttagtca gtaccatcgc
     7921 aatattagta gcataatctc cattttttgt atctttagga acttcaattt taatatcagg
     7981 aatctcatct gctaatcctg ctttgttaat acttgctgca atttcttcta ctaatgtttg
     8041 tttcacttga tcaataatat tcattcgcct atctccttat agttaatttc atattgataa
     8101 gaacccattt tttcattatc ttgatataat tcataatgaa tctttagctt gccaccgtct
     8161 ccacttacga aatgtaaaat gcgtaatgtt ttaacttcta gtggaattcg tccagccgat
     8221 atatcgtaaa aagttgttgt cgtttgtcct tcaacaaaat gcaaattcat attaatgtcg
     8281 cctttacgaa tcaacttaac gctatcatca tccactttta ttgtaacatt aactgttgca
     8341 tcctcaattt gttctacata ccgaataaag ttagattgcc tttgttgcca agttccttca
     8401 gtagtaaatt caaatttttc tttttcgttg tgctgtttca acacttgctt tgtttgaata
     8461 cttacttttt tatccaattt actactcacc ttaatcttcg cataatagaa atattatagc
     8521 ataaaataag gtcatcttgt tatgccttct taaagctaaa taggttcaat taattaacaa
     8581 ttccatactt aatcaacgct tcatatatgt aagccatttt ataaaataca aacaacacct
     8641 caaaaccaaa aaacacgaga ttttcactat ctttcatgaa aacctcgtgt ttgattataa
     8701 tgatagtgtt cagagcacta ccttctttat ctatatagtc agttgatatt tttaaaattt
     8761 aataaccatt ctaccagtta tagtaccatt ttccatttct tcaaaaatat cattgatttc
     8821 ttctaatttt cttaattgaa ctttaggtgt tactttattt tcagcagcaa attcaaacgc
     8881 ttcacgtaag tcttgtcttg taccaacaag tgaacctact acttcaatac catcaagcac
     8941 taatcttggg atatctaagt tcattttatc aacaggtaaa ccaacggcaa caactcttgc
     9001 accagctttt acaacatcaa ccgcttggtt aaatggcgtc ttagcaactg aagttaccac
     9061 tgttgcatct aatcctttgt tatcagttaa tttcataact tctgcaactg gatcaacatc
     9121 tttagaatta ataatagcat cagcacctaa ttctttcgcg aatgctaatt tatcatcatt
     9181 gatgtcgaag gcaacaattt tagcccccat aacgttttta gcatattgta aagctaggtt
     9241 acctaaacca cctataccaa aaacacctaa ccattgtcca ggttttacat tacttacttt
     9301 tacagcttta taagttgtca cacctgcgca tgtaatagaa gacgctgctg ctggatctaa
     9361 tttttcaggt actttcacag catagtctgc agtaacaata acttgttcag ccattgcacc
     9421 atctactgta taaccagcat ttttcacact acggcaaagt gtttcacgac ctgttgtaca
     9481 atattcacat cttccacagc tttcgaacat ccaagcgata gacacacggt ctccaatttt
     9541 taatgattct acatcttccg caacttcgat gactttacca ataccttcat gacctaaagt
     9601 aacgcctgta acatcaccaa aatcagcatt cttaacatgt aaatcggtat gacaaacgcc
     9661 acaatattcc gtttgtacca acgcttcacc aggttttaaa gctcttaact ttttgtcctc
     9721 aatacttact ttgtgatctt tcgttacaac tgctgctctc ataataatgt cctccttata
     9781 gtcactccag tatgtatgat attaattggc tattactttc aaaaaagaaa acgaataatt
     9841 atagatgtaa tttttatttg taatagacgc cagattaaaa catactactg tgaccttgtt
     9901 tttagttaga tatcaaccgt taacttttaa cgtttcccta gttaatatgt taaaagaaat
     9961 gacataatcg atatgctaac gtgtaatttt tacttcacat atatttattt aaagataatt
    10021 tataatactc ttatcttcac tcttattata atgtgaatta attcacaaag tatatagcta
    10081 tgatcaaaaa aatgtgtcag ttattcaaat tttttattaa ctacagatta atttaatttc
    10141 aaaaaaatta aaaaaactcc caatcaatct aagacattat tttcacctca cgatttacgt
    10201 cgtcattgag aaatgaatca tatctttata gattaattgg gaaattttag tttctaatat
    10261 ttaattaaat gcttgatgta aagcatcaga agaatgcttc cacatacctt catcatggtt
    10321 cattaaaaag tcttttaaaa tagctttatt ctcgtcacca atttgatcta tgataatatg
    10381 atgtttcatt gatttatcca tcatattaac atgttcaggc atacttttat aaccacgacg
    10441 aattgcttta ttcactaata aatagcacgc tgttaaacca gcgtaatatg gtccttgttc
    10501 attacgttct gttgttaccc aacacaacca atattcttta gcttcatcgc cttcaacagc
    10561 agctttatca gtaatccatt tcacaccttt ttcaactttg gcacgagcat gcatgcctcc
    10621 aatatcaata aatgcttctt tgttagcaac atcgataaaa actggtgcaa tattgtctaa
    10681 gctaattgaa ccaatatttg tacctttatg accatctaga gggtcatttt ttatgatatt
    10741 aaaattaaag ccttttttct cagacaatga tataacctcc atatttaacg ataacaattt
    10801 tagccctgtt taattaagct cattaatcaa attttcaata gcttctctaa ttgtcttatc
    10861 tttaatcttt gcaaccattt ctctaggata atataattta tagtcttggc gattaatgcc
    10921 tgtaatacta tgaatgacta atgattgatt gcttatttct ctaatcgtac catcttgtct
    10981 taataaatga atcggtttgc gatttgaccc cggtcgatca tagtcatatg gcaaatcaga
    11041 aaatgcttca ctcacaaaat aataatctgg attaatacca cctgcttcaa acagttcttg
    11101 cagttctgat attgtaataa ttgagccatc aaatggaata tatttaaata agtctcgatt
    11161 aataaatcga cttgctaaat cacttaaaat agcatcatct tcttttgtcc atttttccaa
    11221 ataatacgta actaccgctt catcgagttc aacatattgt tcaatcgtaa ctgtctcttc
    11281 aaaaaatgga ataaaatcat gtggatgcaa cttaaattca tagccttcat tataaagctg
    11341 ttttgcgcgt ttcaaacaat tattaagcag cacttctcca ccacgactaa ctgggtggaa
    11401 gtaaatttgc caatacattt gataacgact cataataaag ttttcaactg catgcatacc
    11461 actttctttg attagtactt catctttaga aggtcgcatt aatcttaaaa tacgctccat
    11521 atcaaaagca ccatatgata cacctgtgaa atacgcatca cgttgtaaat aatccattct
    11581 atccgcatcg atttgtgacg aaatcatcga aatgaccaat ttattatgat gcgttttatt
    11641 aattacttcc gcaacttctc ttggaaactc aggcgccact ttacgtaata cagcattcac
    11701 ctcagtatct ccagtaataa tcgcttgtgt gtatgcttca tggtctgtat taaatatttt
    11761 ttcaaaacta tgtgaaaatg gaccatgtcc taaatcatgt aataatgcag cacataatgc
    11821 caacggtcta tctttattgt cccatgcatc atgaccaata aatgactcat caattaatcg
    11881 tctaactatt tcatacacac ctaaagaatg tccaaagcga ctatgttctg ctgtgtgaaa
    11941 agataggtac agtgttccta gttgtctaat tcgacgtaac ctttggaatt ccttagtttt
    12001 aattaaatcc catatcaatt gatcttcaac atgaatatat cgatgtatcg ggtctttaaa
    12061 aactttttct tctactaatt ttaaatctac atatgcgtta gtcattattc ccctcctttt
    12121 cgtttaatat aatatttaat ttacttaaaa tgctttgtac ataagtgcta agtctaactt
    12181 ttcgccatac atttctggct cataagagcg taagattgta aaaccttgct ctttatagta
    12241 agctactgct tcttcatttt tattatctac ttctaagtaa acaccttcaa atttatcttc
    12301 aaaacgtgat aatccttcat ttaacaatgc tgtaccataa cctgtatgtt gcgattctgg
    12361 tttaacataa tgagctgata aatataattc ttcaccgtaa ataaagttag caaagccaac
    12421 gatgtcatta ccttcttcaa cgactaagaa taattgttct tgaagtcttt tctttaaatg
    12481 atgttcatta tatgaagctt ctaacaagtg attaactgtt gtcgcagcgt atatatttaa
    12541 gtatgtatta aaccaagctt tagttgcgac atctctaatt tgaacaacat ctttttcagt
    12601 tgcttgtctt accttgaaca tgactttctc cccttattaa caagttttaa taacggcatt
    12661 ataccacaac ttgctcaata cttaataaac aatgattgtc tattcaattt atatatttat
    12721 attttccgtt aaaattaaaa ataaaaaata acgaagcaaa aaatcacttc gtttagtatg
    12781 aggtatgtct tattgcaata tactattcca ctcagttgca cgtgctaagg catagttgtc
    12841 tttcatgatg tcaccaggct tttcagcagt tccaataata taaccattta aagtggcacc
    12901 taaaaagtct aaactatatt tcatttgcgt aattgctggc ttcgctttta tttttggaca
    12961 atctccacca actaaaataa ctctaaaatc cttttcggcc atttgtgcct taaaattagg
    13021 atatcgttta tcttgtaatg tttctgacca atgttcgata aatgctttca atggtgctga
    13081 aatgctatac caatacactg gtgatgcaaa aataattgta tcactagcca atattttatc
    13141 tagaatcggc aaatagtcat cgtcatatga agtaatagtc tctgctgtat gtctcacgtc
    13201 acgtatcggt ttaaactgat gttgtgtcac gtcaatccat tgatactcta aatcttgcaa
    13261 agcgaatttt gttaattgtg cagtattacc gtttggtcta ctcccaccaa acaaaacagt
    13321 aatcatttta gcctaacctc acttttgatt aataaatatc tgtgtttttc gttacctaat
    13381 tatactatca taagctttgc ctaccgaata gtaaaacgct tacaactttt atataaattt
    13441 gacgaaattt cgtcatgcct tatataacgt cgtttgtgat acggggctaa ttcatgatga
    13501 aattagatac atatatcacc attaaataca attcatttag tcttcaatcg gaaacagttc
    13561 atcgatatat tgaatctcat catctgataa aacgatatct gcagctttaa tattttcaac
    13621 gacttgttct gcacgttttg caccaggaat aatcacatcg atagctggtc tcgttaaata
    13681 aaatgctaat acaatgttcg caattgaagt ttgatgtgct gcagctatgc tttccaaagc
    13741 ttttacgcga cgcacatttt cttcaaatac acctggttta aaatcacgac gtgtagtacg
    13801 atggtcacta aatttagtgt tctcatcata ttttccagct aaaataccgg atgctaatgg
    13861 gaaatatgga ataaatgtga tttggtgatc aacacaatat tgtaatactg cctcattttc
    13921 gcgatgcaat aaattatatt ctaactgtac aacatcaacg taaccatctt tatttgcttc
    13981 tttaagttga tctaatgtga aatttgatac accaattgct ttaatcttcc cttgttcctt
    14041 aagctcttgt aatgctgcaa ctgcttgatc tttcggagtg ttgttatccg gaaaatgaat
    14101 ataatataaa tcgatataat cagtttgtag acgtttcaaa ctattctcaa cttgttgttt
    14161 taaatattcc ggttgattgt tctgatgtac ttcttgattt tcatcaaatt catgagaccc
    14221 tttcgtagca attttaattt gctctcgcgg atattcttta acaacttctc caaccaattc
    14281 ttctgatcgt tctggcccat aaatatatgc cgtatctaat aaattaatac catgattaat
    14341 ggcttgacga acaacatctt ttccttgttc ttcatctaag ttcggatata aattatgccc
    14401 acctactgcg ttcgtcccaa gtgcgattgg aaacacttca acatcagatt tacctaagtt
    14461 tacaaattgc ttcattagac ccagctcctt atctttataa cttttccact atcacactat
    14521 catgatttaa tacaacttcc aaatgttaga attagagctt gaatttaagc ggtgtaatag
    14581 gaaaacgtta tataaaactc agttaaaata agtattgctt aaattgatat gcatatagcg
    14641 cgattgctcg ttgaccttta aattacatgt acttttgaat cagaaattga aaaacattta
    14701 agaacgacaa tattaaagcc attgcaaatt taatttggta atttctattg gtttttaagt
    14761 atgacatgaa tgtttttatc tataattaaa gctatattaa ctagaaaaaa acccattata
    14821 actgcaatcc aaacaaaata tactattatg ttttgataac tcattgataa tagtgcatct
    14881 aggaatagca tagtcaatcc gccaaataaa atatatccaa ataatgtgaa acaaacattt
    14941 tttagtgtag gtattaaaaa aatttttgac cttttatttt tagtattata aatatttaaa
    15001 gatgattttt taattaggcg tacataaaat attaaaatcc ctataaatgt gatgcaacat
    15061 atggcaataa ctaatttttt atcaagttga atgtcaagta aatgtgtata ttttcttaat
    15121 gcaactccca gcaaagcaga gctacctgca actggccaaa aaacattctt tttagcaggt
    15181 ttaacaatat ttaagttttc aaactcttct ctactaattt tgacgtattc ttttgggatg
    15241 aaccaattaa taaaaggaaa aaataaacta atccaagtgc ttattatatc gaccattaag
    15301 tattcatcat tatatttaat aattctatat tttgggtttt tattgatgat tttagattcg
    15361 catagcaaca tttaccagct ccatttctta gggcttatat gattagaaat actttttgaa
    15421 gcttcaccat gacattttca acaccatcta ctgttttttc tgcagatttt tctacagcat
    15481 ctataccaac gtctagttta tgtcctatcc aatcgttttt atcttttatt ccaaattcat
    15541 ttttttgatt ggtccaatat acaagtattg ccgaaataga acctgcagta aatccagcta
    15601 aaccatcatt tttccaccgt gttagtttta gataatacat aaattttttt gtcaataatc
    15661 gatgacatgt ttaacagaaa gaaacctgca gtcattacca accaaccaat aaagcctatt
    15721 atattttgag gatttaaagt taataacata cttagagcca ttattgaaag accgccaaat
    15781 aaaaaataat aaaatattgt gaaacaaaag ttttttaatg aaggaaataa aattattttc
    15841 cctttactag agttattatt ataaatttcc aaccttagct tacgattcag aaataaaaaa
    15901 agtattaaaa cacccagaaa tattgcacaa catattacaa tcaccatgtt tttctctaat
    15961 tgaatattaa gtgaaggaat atattttcta aacgtaactc cgaacaaaat agtactacct
    16021 gcaactggcc aaaaagcttt atttttaaca ggtttgacaa tgtttaaatc gtcaaattct
    16081 tttttactga ttttgacata tttttttgga attaaccaat taatcatagg aagaaataaa
    16141 gttaaccagg tacttactaa atcaatcatt aaatattcat caccatattt aataactcta
    16201 tacttaggat ttttgttgat aactttagat tcacatagca atatttatca ctaccattcc
    16261 tttgtttatt attcctatta aaacttttca attccatttg atttaaataa tacatgaact
    16321 ttattgtcat atagtgagca catatttaaa aataagaaaa acaaaaatat tacaaacaaa
    16381 gttataaata atattatatt ttgaacttca atcgtcaata acatataaat agtaaagaat
    16441 gaaccaccac ctaaataaat atataaaact attgataaac aaagactctt taaagtggga
    16501 aatatgatta tcttattgct tttatttttg ttttcattat agatttgtaa ctttaacttt
    16561 tgatttaaat ataaaaaaaa cagaaaaata cctagtatta tgatgc
//
LOCUS       DAKTGS010000053        16600 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.053, whole genome shotgun sequence.
ACCESSION   DAKTGS010000053 DAKTGS010000000
VERSION     DAKTGS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.053"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            83..4228
                     /gene="sdrD"
                     /locus_tag="O0286_001421"
     CDS             83..4228
                     /gene="sdrD"
                     /locus_tag="O0286_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCY0169804.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKK
                     QNK"
     gene            4622..8104
                     /gene="sdrE"
                     /locus_tag="O0286_001422"
     CDS             4622..8104
                     /gene="sdrE"
                     /locus_tag="O0286_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HCY0169805.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAAL
                     GSLLLFGRRKKQNK"
     gene            8223..9695
                     /locus_tag="O0286_001423"
     CDS             8223..9695
                     /locus_tag="O0286_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0169806.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(9819..11309)
                     /locus_tag="O0286_001424"
     CDS             complement(9819..11309)
                     /locus_tag="O0286_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0169807.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(11591..12469)
                     /locus_tag="O0286_001425"
     CDS             complement(11591..12469)
                     /locus_tag="O0286_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCY0169808.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(12482..13147)
                     /gene="bshB2"
                     /locus_tag="O0286_001426"
     CDS             complement(12482..13147)
                     /gene="bshB2"
                     /locus_tag="O0286_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCY0169809.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(13161..13523)
                     /locus_tag="O0286_001427"
     CDS             complement(13161..13523)
                     /locus_tag="O0286_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCY0169810.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            13799..14557
                     /gene="nagB"
                     /locus_tag="O0286_001428"
     CDS             13799..14557
                     /gene="nagB"
                     /locus_tag="O0286_001428"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCY0169811.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            14634..15266
                     /gene="hxlA"
                     /locus_tag="O0286_001429"
     CDS             14634..15266
                     /gene="hxlA"
                     /locus_tag="O0286_001429"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCY0169812.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            15268..15816
                     /gene="hxlB"
                     /locus_tag="O0286_001430"
     CDS             15268..15816
                     /gene="hxlB"
                     /locus_tag="O0286_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCY0169813.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            15929..16576
                     /locus_tag="O0286_001431"
     CDS             15929..16576
                     /locus_tag="O0286_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0169814.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
BASE COUNT         6284 a         2586 c         2883 g         4847 t
ORIGIN      
        1 aaatgtaata ctatattgat atacattttt gtatttaaaa caattgtatt atttaaaaat
       61 ttgatgaatt aggagtaatc taatgctaaa cagagaaaat aaaacggcaa taacaaggaa
      121 aggcatggta tccaatcgat taaataaatt ttcgattaga aagtacacag tgggaacagc
      181 atcaatttta gtaggtacaa cattaatttt tggtctgggg aaccaagaag caaaggctgc
      241 agaaagtact aataaagaat tgaacgaagc gacaacttca gcaagtgata atcaatcgag
      301 tgataaagtt gatatgcagc aactaaatca agaagacaat actaaaaatg ataatcaaaa
      361 agaaatggta tcatctcaag gtaatgaaac gacttcaaat gggaataaat taatagaaaa
      421 agaaagtgta caatctacca ctggaaataa agttgaagtt tcaactgcca aatcagatga
      481 gcaagcttca ccaaaatcta cgaatgaaga tttaaacact aaacaaacta taagtaatca
      541 agaagcgtta caacctgatt tgcaagagaa taaatcagtg gtaaatgttc aaccaactaa
      601 tgaggaaaac aaaaaggtag atgccaaaac tgaatcaact acattaaatg ttaaaagtga
      661 tgctatcaag agtaatgatg aaactcttgt tgataacaat agtaattcaa ataatgaaaa
      721 taatgcagat atcattttgc caaaaagtac agcacctaaa cgtttgaata caagaatgcg
      781 tatagcagca gtacagccat catcaacaga ggctaaaaat gttaatgatt taatcacatc
      841 aaatacaaca ttaactgtcg ttgatgcaga taaaaacaat aaaatcgtac cagcccaaga
      901 ttatttatca ttaaaatcac aaattacagt tgatgacaaa gttaaatcag gtgattattt
      961 cacaattaaa tactcagata cagtacaagt atatggattg aatccggaag atattaaaaa
     1021 tattggtgat attaaagatc caaataatgg tgaaacaatt gcgactgcaa aacatgatac
     1081 tgcaaataat ttaattacat atacatttac agattatgtt gatcgattta attctgtaca
     1141 aatgggaatt aattattcaa tttatatgga tgctgataca attcctgtta gtaaaaacga
     1201 tgttgagttt aatgttacga taggtaatac tacaacaaaa acaactgcta acattcaata
     1261 tccagattat gttgtaaatg agaaaaattc aattggatca gcgttcactg aaacagtttc
     1321 acatgttgga aataaagaaa atccagggta ctataaacaa acgatttatg taaatccatc
     1381 ggaaaattct ttaacaaatg ccaaactaaa agttcaagct taccactcaa gttatcctaa
     1441 taatatcggg caaataaata aagatgtaac agatataaaa atatatcaag ttcctaaagg
     1501 ttatacatta aataaaggat acgatgtgaa tactaaagag cttacagatg taacaaatca
     1561 atacttgcag aaaattacat atggcgacaa caatagcgct gttattgatt ttggaaatgc
     1621 agattctgct tatgttgtaa tggttaatac aaaattccaa tatacaaata gcgaaagccc
     1681 aacacttgtt caaatggcta ctttatcttc aacaggtaat aaatccgttt ctactggcaa
     1741 tgctttagga tttactaata accaaagtgg cggagctggt caagaagtat ataaaattgg
     1801 taactacgta tgggaagata ctaataaaaa cggtgttcaa gaattaggag aaaaaggcgt
     1861 tggcaatgta actgtaactg tatttgataa taatacaaat acaaaagtag gagaagcagt
     1921 tactaaagaa gatgggtcat acttgattcc aaacttacct aatggagatt accgtgtaga
     1981 attttcaaac ttaccaaaag gttatgaagt aaccccttca aaacaaggta ataacgaaga
     2041 attagattca aacggcttat cttcagttat tacagttaat ggcaaagata acttatctgc
     2101 agacttaggt atttacaaac ctaaatacaa cttaggtgac tatgtctggg aagatacaaa
     2161 taaaaatggt atccaagacc aagatgaaaa aggtatatct ggcgtaacgg taacattaaa
     2221 agatgaaaac ggtaacgtgt taaaaacagt tacaacagac gctgatggca aatataaatt
     2281 tactgattta gataatggta attataaagt tgaatttact acaccagaag gctatacacc
     2341 gactacagta acatctggta gcgacattga aaaagactct aatggtttaa caacaacagg
     2401 tgttattaat ggtgctgata acatgacatt agatagtgga ttctacaaaa caccaaaata
     2461 taatttaggt aattatgtat gggaagatac aaataaagat ggtaagcagg attcaactga
     2521 aaaaggtatt tcaggcgtaa cagttacatt gaaaaatgaa aacggtgaag ttttacaaac
     2581 aactaaaaca gataaagatg gtaaatatca atttactgga ttagaaaatg gaacttataa
     2641 agttgaattc gaaacaccat caggttacac accaacacaa gtaggttcag gaactgatga
     2701 aggtatagat tcaaatggta catcaacaac aggtgtcatt aaagataaag ataacgatac
     2761 tattgactct ggtttctaca aaccgactta caacttaggt gactatgtat gggaagatac
     2821 aaataaaaac ggtgttcaag ataaagatga aaagggcatt tcaggtgtaa cagttacgtt
     2881 aaaagatgaa aacgacaaag ttttaaaaac agttacaaca gatgaaaatg gtaaatatca
     2941 attcactgat ttaaacaatg gaacttataa agttgaattc gagacaccat caggttatac
     3001 accaacttca gtaacttctg gaaatgatac tgaaaaagat tctaatggtt taacaacaac
     3061 aggtgtcatt aaagatgcag ataacatgac attagacagt ggtttctata aaacaccaaa
     3121 atatagttta ggtgattatg tttggtacga cagtaataaa gacggcaaac aagattcaac
     3181 tgaaaaaggt atcaaagatg ttaaagttac tttattaaat gaaaaaggcg aagtaattgg
     3241 aacaactaaa acagatgaaa atggtaaata ctgctttgat aatttagata gcggtaaata
     3301 caaagttatt tttgaaaagc ctgctggctt aacacaaaca ggtacaaata caactgaaga
     3361 tgataaagat gcagatggtg gcgaagttga cgtaacaatt acggatcatg atgatttcac
     3421 acttgataat ggatactacg aagaagaaac atcagatagc gactcagatt cggacagcga
     3481 ctcagattca gacagagact cagactcaga tagtgattca gactcggata gcgattcaga
     3541 ttcagacagc gattcagatt cagatagcga ttcagattca gacagagact cagatagtga
     3601 ttcagactca gatagcgact cagattcaga cagcgactca gattcagaca gcgactcaga
     3661 ctcagatagt gattcagact cagatagcga ctcagattca gacagcgact cagactcaga
     3721 cagcgactca gactcagata gtgactcaga ttcagatagc gactcagatt cggacagcga
     3781 ttcagactca gatagcgact cagattcaga tagcgattcg gactcagata gcgactcaga
     3841 ttcagatagt gattcagact cagatagcga ctcagattca gacagcgact cagattcgga
     3901 tagcgactca gattcagaca gcgactcaga ctcggatagt gattcagact cagatagcga
     3961 ctcagactca gatagcgatt cagattcaga tagcgactca gactcagaca gcgattcaga
     4021 ctcagacagc gactcagact cagatgcagg taagcacaca cctgttaaac caatgagtac
     4081 tactaaagac catcacaata aagcaaaagc attaccagaa acaggtaatg aaaatagcgg
     4141 ctcaaataac gcaacgttat ttggcggatt attcgcagca ttaggatcat tattgttatt
     4201 cggtcgtcgt aaaaaacaaa ataaataata cagcttatac caggtccgtg aggcctggtt
     4261 ttttatttca aaatttatga aatgtatctt atgtttgaga ttaaatagtg caatttttta
     4321 aaaaatataa cataaatcaa cataaattta attcatcttt acattaaaag tacaaacgaa
     4381 taagaaaagc aattaaacac agtatcaaac catttaaaac aattgatata aaaaacaaac
     4441 gaataattaa aaagtactag aattaattta ttcaaaccaa agtgtaaaat aacagtctta
     4501 gataaataaa tttatttaaa gtattgtgct ttatctaaaa atgtattacg atgggaatac
     4561 aaatttttat atgtaaaaat attaattttt tgcaaatatt gatattttaa aggagattta
     4621 tatgattaac agggataata aaaaggcaat aacaaaaaag ggtatgattt caaatcgctt
     4681 aaacaaattt tcgattagaa agtatactgt aggaactgca tcgattttag taggtacgac
     4741 attgattttt ggtctaggga accaagaagc taaagctgct gaaaacacta gtacagaaaa
     4801 tgcaaaacaa gatgatgcaa cgactagtga taataaagaa gtagtgtcgg aaactgaaaa
     4861 taattcgaca acagaaaata attcaacaaa tccaattaag aaagaaacaa atactgattc
     4921 acaaccagaa gctaaaaaag aatcaacttc atcaagtact caaaaacagc aaaataacgt
     4981 tacagctaca actgaaacta agcctcaaaa cattgaaaaa gaaaatgtta aaccttcaac
     5041 tgataaaact gcgacagaag atacatctgt tattttagaa gagaagaaag caccaaataa
     5101 tacaaataac gatgtaacta caaaaccatc tacaagtgaa ccatctacaa gtgaaattca
     5161 aacaaaacca actacacctc aagaatctac aaatattgaa aattcacaac cgcaaccaac
     5221 gccttcaaaa gtagacaatc aagttacaga tgcaactaat ccaaaagaac cagtaaatgt
     5281 gtcaaaagaa gaacttaaaa ataatcctga gaaattaaaa gaattggtta gaaatgatag
     5341 caatacagat cattcaacta aaccagttgc tacagctcca acaagtgttg caccaaaacg
     5401 tgtaaacgca aaaatgcgct ttgcagttgc acaaccagca gcagttgctt caaacaatgt
     5461 aaatgattta attaaagtga cgaagcaaac aatcaaagtt ggcgatggta aagataatgt
     5521 ggcagcagcg catgacggta aagatattga atatgataca gagtttacaa ttgacaataa
     5581 agtcaaaaaa ggcgatacaa tgacgattaa ttatgataag aatgtaattc cttcggattt
     5641 aacagataaa aatgatccta tcgatattac tgatccatca ggagaggtca ttgctaaagg
     5701 aacatttgat aaagcaacta agcaaatcac atatacattt acagactatg tagataaata
     5761 tgaagatata aaatcacgct taactctata ttcgtatatt gataaaaaaa cagttccaaa
     5821 tgagacaagt ttgaatttaa catttgctac agcaggtaaa gaaacaagcc aaaatgtcac
     5881 tgttgattat caagatccaa tggtccatgg tgattcaaac attcaatcta tctttacaaa
     5941 attagatgaa gataagcaaa ctattgaaca acaaatttat gttaacccat tgaaaaaatc
     6001 agcaaccaac actaaagttg atatagctgg tagtcaagta gatgattatg gaaatattaa
     6061 actaggaaat ggtagcacca ttattgacca aaatacagaa ataaaggttt ataaagttaa
     6121 ctctgatcaa caattgcctc aaagtaatag aatctatgat tttagtcaat acgaagatgt
     6181 aacaagtcaa tttgataata aaaaatcatt tagtaataat gtagcaacat tggattttgg
     6241 tgatattaat tcagcctata ttatcaaagt tgttagtaaa tatacaccta catcagatgg
     6301 cgaactagat attgcccaag gtactagtat gagaacaact gataaatatg gttattataa
     6361 ttatgcagga tattcaaact tcatcgtaac ttctaatgac actggcggtg gcgacggtac
     6421 tgttaaacct gaagaaaagt tatacaaaat tggtgactat gtatgggaag acgttgataa
     6481 agacggtgtt caaggtacag attcaaaaga aaaaccaatg gcaaacgttt tagttacatt
     6541 aacttacccg gacggtacta caaaatcagt aagaacagat gctaatggtc attatgaatt
     6601 cggtggtttg aaagacggag aaacttatac agttaaattc gaaacgccaa ctggatatct
     6661 tccaacaaaa gtaaatggaa caactgatgg tgaaaaagac tcaaatggta gttcggttac
     6721 tgttaaaatt aatggtaaag atgatatgtc tttagatact ggtttttaca aagaacctaa
     6781 atacaactta ggtgactatg tatgggaaga tactaataaa gatggtatcc aagatgcaaa
     6841 tgagccagga atcaaagatg ttaaggttac attaaaagat agtactggaa aagttattgg
     6901 tacaactact actgatgcct cgggtaaata taaatttaca gatttagata atggtaacta
     6961 tacagtagaa tttgaaacac cagcaggtta cacgccaacg gttaaaaata ctacagctga
     7021 tgataaagat tctaatggtt taacaacaac aggtgtcatt aaagatgcag ataatatgac
     7081 attagacagt ggtttctata aaacaccaaa atacagttta ggtgattatg tttggtacga
     7141 cagtaataaa gacggcaaac aagattcaac tgaaaaaggt atcaaagatg tgacagttac
     7201 attgcaaaac gaaaaaggcg aagtaattgg aacaactaaa acagatgaaa atggtaaata
     7261 tcgtttcgat aatttagata gcggtaaata caaagttatt tttgaaaagc ctgctggctt
     7321 aacacaaaca gttacaaata caactgaaga tgataaagat gcagatggtg gcgaagttga
     7381 cgtaacaatt acggatcatg atgatttcac acttgataac ggatacttcg aagaagatac
     7441 atcagacagc gattcagact cagatagtga ctcagacagc gactcagact cagacagcga
     7501 ctcagactca gacagtgatt cagattcaga cagcgactca gattcagaca gcgactcaga
     7561 ttcagatagc gactcagatt cggacagcga ttcagactca gatagcgact cagattcaga
     7621 tagcgattca gactcagaca gcgactcaga ttcagatagc gattcggact cagacagcga
     7681 ttcagactca gatagcgact cagactcaga cagcgactca gattcagata gcgattcgga
     7741 ctcagatagc gactcagatt cagacagcga ttcagactca gatagcgact cagactcaga
     7801 cagtgattca gattcagaca gcgactcaga ctcagatagc gactcagatt cggacagcga
     7861 ctcagactct gatagcgact cagactcaga cagtgattca gacagcgatt cagactcgga
     7921 tgcaggaaaa catacacctg ttaaaccaat gagtactact aaagaccatc acaataaagc
     7981 aaaagcatta ccagaaacag gtagtgaaaa taacggctca aataacgcaa cgttatttgg
     8041 tggattattt gcagcattag gttcattatt gttattcggt cgtcgcaaaa aacaaaacaa
     8101 ataatacaat atgacccagg tccttgtggc ctggtttttt tataattaca catgcaatag
     8161 atgtattttt catataaaat aaacaataaa gatacggaat aaaacttata tgaggcgata
     8221 atatgaatta cattttagga acaattttag aaagtaaaat tacaggtgta gaaaaagcgc
     8281 aaataaatag attgaagttg ttcaaacaac acggcatatc ttcaaaatgt gtatatgtta
     8341 aatggaatcc ttattcatac acatatgcga agcaacatca gattgaaaat gatgtattta
     8401 caatgtatga ctattttcaa aaagcaatca attataaaaa gacaaagcaa gttaactgga
     8461 tacagtattg ggaaaagtca tgtaggtaca cattgaaatt tgtggaaaat tcaaatgatg
     8521 tcagaatata tgatgaagag caatttgtaa tgtatgctca ttttttagat aaacagtatc
     8581 atcaattaaa ttatgtgaat tattttgatc ataaaagaag aaaagtaaaa cgcgaattgt
     8641 atgatggaag aggcttttta agttgttctc gaattttagg tgaaggacaa cggattgtac
     8701 tcgaaaatta ctatacacct aatggggaaa tcgtcatcca aaaatatttt gacgatataa
     8761 aagggaaaaa cacgctcaca aaggttatct taaatgaaga ccagcatcaa caattttttg
     8821 atacagaaga tgaattagtt caatattttc tccatcaatt atgtaaaaat aatgatcaaa
     8881 tcatattaga tcgtcctcat gaattaggaa atgttatagc gggattaaat caaagtattc
     8941 cagttattgt tgtactccat agtacacatt tatccggtgc cggtaatggt ataaaaagtt
     9001 tttataaaac agtgtttaat aatttaacac gttataaagc gattgttgta tcaacagaaa
     9061 agcaatgcca agatatttca caatatattg aaaataaaat accagttatc aatattccgg
     9121 ttggctacgt ggcaaattta aagtatcaat ttgacatcaa tcaaaaggag aaaaatcata
     9181 tcatatcaat tgctcgcctc gttgaaaata aacaaattaa acatcaaatt gaagtaatca
     9241 agcaattagt aacaaaacat cccaatattc aattgaatat ttatggacat ggaaatggtt
     9301 tgtcagaata tcgacaactt gtagaagatt atcatttatc ggaacatgtt aaatttcatg
     9361 gttttaagac gcatattaat gaagagattg ctaaagcaga actgatgtta tcgacaagta
     9421 aaatggaagg ttttggctta gcaattttag agtcgctttc agtaggtaca ccagtgatca
     9481 gttatgatgt agattatggt ccatcagaac tgattcaaga tggatttaat ggctatttag
     9541 tacctcaagg tgacatcaat caaatggttg aaaaggtcga ccaattacta aataatactc
     9601 aaaaattgca acagttttca attaatagca tagaatctgc acaacagtac aatgcaacta
     9661 ctatcagtac aaagtggcaa aatattttaa actaagtcaa aagaaaaaag cattttccta
     9721 gaatcatcta tttaacaaac ctcgttcata cgaacttaca tgtttagtta aatgacatag
     9781 gaaaatgcta tttttgtaaa aataaaggta aagtacattt atataagacg aacaaattgg
     9841 tcccattgtt taattaacga cgctttacta tattgttgtg cttttgccaa actacctttt
     9901 gacagtcgtt gctgtacttc aggatgatca atcacatatt ttactttatc aaatagggca
     9961 tcttcatcat ttttagtaat taaataacca ttgaaatctg aagtaatcag ttcgttaggt
    10021 ccatatttaa tatcataact aataactgga acaccatgtg ctaaagattc aagtagcgct
    10081 aaagagaaac cttccatgtt acttgttatt aaactcaaat aggcatcgct atattcttgg
    10141 tctagattgc ttaaaaagcc gcgtaagtaa acatgatttt ccaatccata tttttgtatc
    10201 aattcattta attttttact ttcagaacca aaaccataca tatgaagctc tatttttggg
    10261 acatacgata ctaagcgttt aattaattca atttgttgat gtaattgttt ttcaggtgaa
    10321 taacgagcaa cggaaattaa tttaacactg cgctgatcta atgtttggac tggtgtatca
    10381 attgtttcac tatagccgac aggaatatta acaactggaa tagtatggtt aatacgtttt
    10441 tcaacatcta atttttgctg ctcagtagaa acgataattg cacgatatcg agataaattt
    10501 tcaaacatcg ctttatatac atttttaaat ggcgatgaat ctaatgcatc aatattttta
    10561 atgtgtgtac tgtgaagcac agctactact gggattgact caggcgttaa gttgaaaata
    10621 ggtgctgtgt acacattacg atcactgaaa aataaatccc catgttgata tagttgttta
    10681 atgaaaaatg cgcctaattc cgtttcatta ttaaagaaat attgtttgtt agcatagtaa
    10741 acaataattt tttgtacttc tggtttgcca tccttgtaag aaaaatactt ttctaatttt
    10801 gtgtcacctt ctggattata gaaaaattca cataatgttt gttgtttatc aacaagaatc
    10861 ctactacaac ttaaaaagcc acgcacatca taaaaatcac gttttacttt tcgtctttga
    10921 ctatcaaaat gatttacata atctaatata cgatatttag gatcttgaaa atgggcatac
    10981 attaagaaac gctcttgatc atatattcta aagtcatgac tattttcaac atgttttaaa
    11041 gtataatgac attcatcagt ccaatacgac aaccagtcaa atggttcatt gcgttctaaa
    11101 tatgttgctt cttggaagaa atcatacata ttaatatagt cagaactagt aatataattt
    11161 tgggcatttc tatataaata tctattccat gacagaaata cacattgcgc tggtcttccc
    11221 atttctttaa ataaatttaa acgattaata attgctttct ctatcccagt taaattaaca
    11281 cctaaactat tacctacaaa ataattcatt tacaacacca cttatatcta ttttttataa
    11341 ttatatcaca taatatttaa ttacttcttt taactggaag atgtgtttat ttataaaaca
    11401 acaaattttg atatttataa tgatagtagt tattcaatca actacgacca atatatcatt
    11461 gtagagctta ggatattgat ttatgactca ggcacatcaa atgagaagat ttataaaaga
    11521 gatatacaac tctagaaggt ataataaaaa cgcgcaacta atgttacgcg tttgaattaa
    11581 tcatatgata ttatttgcga tactttaatt tagcgaaagc atcatgttga tggatagact
    11641 cttcattacg acattcgata tcgaaaccgt ctaaccaatc aaattcaact aagtccgcgg
    11701 caattaaacg aattaagtct tcgacaaaac gtggattttc atatgcacgc tctgtcacac
    11761 gtttttcatc aggacgtttt aaaatagggt atagaattga acttgcatta gcttccattg
    11821 catctaaaat tttattttta tagtcatcaa ctatgtcttg atctttatta atatatgttt
    11881 taacagtgac aacaccacgt tggttgtgcg ctgaatactc acttatttct tttgaacaag
    11941 ggcatagcgt tgtgacagtt gcttcaatag taagttcttt acgtgtaact ttatcaccgt
    12001 caattgctaa tccataagtg acatcggcat taccaactgc tttaatattt gtggttggac
    12061 tatagcgatc aaagaaccat ttcccagaaa catcaacgcc tgccgcattt tgtttcatat
    12121 tcgtttgtaa agtgcgtaac acctgataaa gtgtattaaa ttcaagttca ataccattat
    12181 catagtgctt ttcaacactt tcgattatac ggctcatatt aatacctttt tcgtcttttg
    12241 ttaaacttgt tgaaaaacta aatgtgccag ctgtttgata ctggtcaaca agtacagggt
    12301 acactaagtt tttaatacca acttcttcta tttcaaataa aaaatcttta tgtgtacttt
    12361 gtaaatctgt catttcgttc ttagtagtag gtttcgtgcc ttcaatagga tctacggaac
    12421 caaagtgttt ccaacgacct tctcgtgtcg ataaatcaaa ttcagtcatt tttttcctcc
    12481 gttaagattt aaagtgatat gtccaatatg gttcgactgt taaaaagctg tgttgtttac
    12541 catcgatttc aggacttgct aattgtttta aaaatggacc tgtttgagaa gcatgtgctt
    12601 caaatgcctt aattttaagt tctttaaaat ctgtaatatc attttgaata tcaggttctc
    12661 caagagcttc ggttgcatca ttactgaacg caactaaagt taaacgaggg cgttcttctt
    12721 taggcatgcg ttcaaccgtt cgaattacag cgtctgctgt tgcttcgtga tcaggatgta
    12781 ctgcatatcc aggataaaat gaaataatca atgatggatt tgtatcatcg attaaagatt
    12841 taatcatacc atctatatgt tcatagggtt caaattcgac agttttgtca cgtaaaccca
    12901 tttttcttaa atcagtaata ccgataactt tacaagcttc ttctagttca cgctcacgaa
    12961 tacttggtaa tgattcgcgt gttgcaaatg ggggattacc taaatttctg cccatttgtc
    13021 ctagggttaa acatgcatat gttacaggta tgcctttttg gatataattt gctaatgtgc
    13081 ctgcagatga gaaggtttca tcatcaggat gtggaaatat tactaataca tgtctttcgt
    13141 cagtcatgtt gatgcctcct ctataaatta aatggtcgct cactaatttg aagtgctgca
    13201 gcgagttgac cttcgtaatt aaaacctgca attaaaaatt catcatgctc attgacctca
    13261 aaatgcgtta gaccttgtac ataaacccaa ccaccatttg atagtttaag accaatgcga
    13321 taaggttctt tattaccacc ttttagttgt gcatgcgtat atgttatttg tatgtttctt
    13381 aaaaaagtac cagcattaaa aacacgttga tcgaaatggt tcgcataggc cccatttgtc
    13441 gtttcaacat gcagatacac aggtttatgt tcaaaagaag caagtaaatc tataacttct
    13501 tgttctttaa ttggttccaa cacgttcact ccttacacta tcaatgtgtt tatctttcta
    13561 ttttactaaa aactattcga taattgtata cgattgctca attatttata aattaatttt
    13621 catgaagggt aattactcag gattacgtaa tcatacagca ttagtttttt acttttaaaa
    13681 atcaaaaatt tgttggaatt tgaaaagtgt taaacattaa aaatgatgct atattaatgg
    13741 tgtatgaatg aattcataag tttttaaaat gtattaaatt tgtggaggca tgtaaacaat
    13801 gaaagtatta aacttaggat cgaaaaaaca agcatcattc tatgttgcat gtgagttata
    13861 taaagagatg gcatttaatc agcactgtaa actaggttta gcaactggtg gtacaatgac
    13921 agatttgtat gaacaacttg ttaagttatt aaataaaaat cagttaaacg tagacaatgt
    13981 atccacgttt aatttagacg aatatgtagg tttaaccgca tcacatccgc aaagttatca
    14041 ctattatatg gatgacatgc ttttcaaaca atatccttat tttaatagaa agaacattca
    14101 tattccaaat ggagatgccg atgatatgaa tgcggaagcg tcaaaatata atgacgtttt
    14161 agaacaacaa ggtcaacgtg atattcaaat tttaggtatt ggtgaaaatg gtcatattgg
    14221 atttaatgaa cctggtacgc cgtttgatag cgttactcat atcgttgatt tgactgaaag
    14281 tactattaag gctaatagtc gatattttaa aaacgaagat gatgttccaa agcaagccat
    14341 ttcgatggga cttgctaata ttcttcaagc caaacgtatc attttactcg catttggtga
    14401 aaagaaacgt gctgctatta cacatttatt aaatcaggaa atttctgttg atgttccagc
    14461 cacattactt cacaaacacc cgaatgttga gatatattta gacgacgaag cttgcccgaa
    14521 aaatgttgcg aaaattcatg tcgatgaaat ggattgattg caatgtttaa ttaagaaatg
    14581 cctcgggaaa ggttccaata gaaagataaa aagcattgga aggatgattt ttagtggaat
    14641 tacaattagc aattgattta ttaaacaaag aagacgcggc tgagttagca aataaagtaa
    14701 aagattatgt agatatcgta gaaatcggta cgccaatcat ttacaacgaa ggtttaccag
    14761 cagttaaaca tatggcagac aacattagta atgtaaaagt attagcagac atgaaaatta
    14821 tagatgcagc tgattatgaa gttagccaag caattaaatt tggcgcggat gtaattacaa
    14881 tactaggtgt tgcagaagat gcatcaatta aagcagctat tgaagaagct cataaaaata
    14941 ataaacaatt actagttgat atgattgctg ttcaagattt agaaaaacgt gcaaaagaac
    15001 tagatgaaat gggtgctgat tatattgcag tacacactgg ttatgattta caagcagaag
    15061 ggcaatcacc attagaaagt ttaagaaccg ttaaatctgt tattaaaaat tctaaagttg
    15121 cagtagcagg tggaattaaa ccagatacaa ttaaagatat tgtcgctgaa agtcctgatc
    15181 ttgttattgt tggtggcgga atcgcaaatg cagatgatcc agtagaagct gcaaaacaat
    15241 gtcgcgctgc aatcgaaggt aagtaatatg gctaaattta gtgactatca attaattcta
    15301 gatgaattaa agatgacttt gtcacatgtt gaagcggatg agttttcaac ttttgcatcc
    15361 aaaatactac atgctgaaca tatatttgta gctggcaaag gacgttcagg attcgtggcg
    15421 aatagttttg caatgcgctt aaatcagctc ggcaaacagg cacatgttgt tggagaatca
    15481 acgacacctg cgattaagtc gaatgatgta tttgtaatta tctctggttc aggttccacg
    15541 gaacatttaa gattattagc agacaaagca aaatcagtag gtgctgacat cgtattaatt
    15601 actacaaata aagattctgc aataggcaat ctagctggga cgaacatcgt tttgcctgca
    15661 ggtacaaaat atgatgaaca aggctcggca caaccattag gaagtttgtt tgaacaagca
    15721 tctcaattat ttttagatag tgttgtaatg ggattgatga ctgaaatgaa tgttacggaa
    15781 caaacgatgc aacaaaatca tgctaattta gaataaaata aagatagtcg ataatatgat
    15841 gcctaggcag aaatattatc gattattttt ttatttaaat aataaattat agtataatat
    15901 caataataaa cgaatagggg tgttaatatt gaagtttgac aattatattt ttgattttga
    15961 tggtacgttg gcagacacga aaaaatgtgg tgaagtagca acacaaagtg catttaaagc
    16021 atgtggctta acggaaccat catctaaaga aataacgcat tatatgggaa tacctattga
    16081 agaatcattt ttaaaattag cagaccgacc attagatgaa gcagcattag caaagttaat
    16141 cgatacattt agacatacat atcaatctat tgaaaaggac tatatttatg aatttgcggg
    16201 tataactgaa gccattacaa gtttgtataa ccaagggaaa aaacttttcg tggtgtctag
    16261 taagaagagt gatgtattag aaagaaattt atcggctatt ggattaaatc acttgattac
    16321 cgaagctgtt ggatccgatc aagtaagtgc atataaacca aatcctgaag gcatacacac
    16381 aattgtgcaa cgctacaatt taaatagcca acaaacggtg tatattggtg attcaacgtt
    16441 tgatgttgag atggcacaac gtgctggtat gcaatctgca gctgtcactt ggggtgcaca
    16501 tgatgcaagg tcattacttc attcaaatcc ggattttatt attaatgatc catcagaaat
    16561 taataccgta ttataaaact tgttaaaaca gagaatacca
//
LOCUS       DAKTGS010000054        16371 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.054, whole genome shotgun sequence.
ACCESSION   DAKTGS010000054 DAKTGS010000000
VERSION     DAKTGS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.054"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            41..502
                     /locus_tag="O0286_001432"
     CDS             41..502
                     /locus_tag="O0286_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCY0169815.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            507..2708
                     /locus_tag="O0286_001433"
     CDS             507..2708
                     /locus_tag="O0286_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCY0169816.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            2765..3739
                     /gene="holA"
                     /locus_tag="O0286_001434"
     CDS             2765..3739
                     /gene="holA"
                     /locus_tag="O0286_001434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCY0169817.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(3784..4035)
                     /locus_tag="O0286_001435"
     CDS             complement(3784..4035)
                     /locus_tag="O0286_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCY0169818.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            4381..6204
                     /gene="lepA"
                     /locus_tag="O0286_001436"
     CDS             4381..6204
                     /gene="lepA"
                     /locus_tag="O0286_001436"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCY0169819.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            6767..7891
                     /locus_tag="O0286_001437"
     CDS             6767..7891
                     /locus_tag="O0286_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCY0169820.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            7991..8968
                     /gene="hrcA"
                     /locus_tag="O0286_001438"
     CDS             7991..8968
                     /gene="hrcA"
                     /locus_tag="O0286_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCY0169821.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            9000..9626
                     /gene="grpE"
                     /locus_tag="O0286_001439"
     CDS             9000..9626
                     /gene="grpE"
                     /locus_tag="O0286_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCY0169822.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            9695..11527
                     /gene="dnaK"
                     /locus_tag="O0286_001440"
     CDS             9695..11527
                     /gene="dnaK"
                     /locus_tag="O0286_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCY0169823.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            11663..12802
                     /gene="dnaJ"
                     /locus_tag="O0286_001441"
     CDS             11663..12802
                     /gene="dnaJ"
                     /locus_tag="O0286_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCY0169824.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            12806..13744
                     /gene="prmA"
                     /locus_tag="O0286_001442"
     CDS             12806..13744
                     /gene="prmA"
                     /locus_tag="O0286_001442"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCY0169825.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            13746..14498
                     /locus_tag="O0286_001443"
     CDS             13746..14498
                     /locus_tag="O0286_001443"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCY0169826.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            14505..15851
                     /gene="mtaB"
                     /locus_tag="O0286_001444"
     CDS             14505..15851
                     /gene="mtaB"
                     /locus_tag="O0286_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCY0169827.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            16144..16320
                     /locus_tag="O0286_001445"
     CDS             16144..16320
                     /locus_tag="O0286_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCY0169828.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT         6110 a         2197 c         3036 g         5028 t
ORIGIN      
        1 agaaaatgtt actaaatttt aagtatggaa ggtgaacatc ttggaaagaa tcaaatggga
       61 agaatatttt atggcacaaa gtcatttgct agcattacgt tcaacttgtc aaagattatc
      121 tgtaggtgca acgattgtta aggataatcg tattattgct ggtggttata atggctctgt
      181 agctggcgag gtgcattgta tagatgaagg atgtttaatt gaagatggac attgtatcag
      241 aacgatacat gcagaaatga atgctttatt acaatgtgca aaacaaggtg tatctactga
      301 aggtgcaaca atctatgtta ctcattttcc atgcctaaat tgtacaaagt caattattca
      361 agcaggtata aagcgtatct actatgcaga agattatcat aaccatgaat atgcaactaa
      421 attactcaaa caatctggta ttgaatttaa aaaaattcca ttttcaccag aatatgttgc
      481 taaatatctg actaaaggtt aatgttttgc tgtttgtcgc gttatcaatg attgtaggag
      541 tgctttggaa ttctagcaaa gtgctctcta catttctttt cattttactt ttgtatatta
      601 cttatcgtaa aaataaaatc gtttatgccc ctatttctct ctttttaatc attttctccg
      661 catggtattt acattattca caacaagcaa tatttaatta tatcaattat attgaacgta
      721 attctcagtt taatgagcgt gctcaagtaa tccaaattca acgtcaaggt agtgacacat
      781 ataaaggtag gttgagttta aaaaatgaaa tatatccttt ctttttaaca gataaaaaga
      841 attttgattt aaagaaaatt gaaagtcgta attgtattgt taaaggacaa ttcaaagtta
      901 atgacaataa gtttgtaact cttaaattac aaagtatagt tgtacaaagc tgcctagaat
      961 cgaaccggtc taatttaatt gagaaacata aacagtttat aatgaatcga atttatgatt
     1021 cgggtattaa gtttccggat cgtattatgg cattgattac tggtgacgta aaagaagtta
     1081 atgagcaatt taaggaacgt gttaaagaga tagttatata tcatttgctg gcagttagtg
     1141 gctcgcatat agctgcaatt gtatttttaa tttaccaacc tttaaaacga ttaaatttac
     1201 ctttatttgt cattaaagga attacaatca ttgtattagc tttatttgct caatacacaa
     1261 attatgcacc tagtgctgta agagctataa taatgacaac tcttgtactg gttattacta
     1321 agcaaattaa aataaagagt attcagctat tagcatttgc atttataatt atgtttattt
     1381 taaatccact agttgtttat gatattggat ttcaattttc attcatcatt tcatttttta
     1441 ttatgctact ttttcctttt ttacagcaat tgtcaaagtt acaatcatta ttcataatta
     1501 cgtttattgc acaattagct tcatttatcg ttgccattcc aagctttcat caacttcaat
     1561 gggtgggatt tttatctaat ttgatttttg taccgtacta ttcgattata ttgtttccgc
     1621 tatctatttt attctttatt acaagtcatt ttattgtggg attaacgccg ctaaattact
     1681 tggttgacct aagttttaat tttcatgact ggttactaga cctattcaca agaatcaagc
     1741 aatcacattt ttctgttccc aagtttaatg attggatatt tatagtattt ataatttctg
     1801 tttattacat attttggtta ttggctaaac gtaaatatat attggttacg ttttggacta
     1861 taattattct gacattatta ataacgtttc caacaaattc acatcacaaa attacaatgt
     1921 taaatgtggg gcagggagac agtattttat atgaaggcgg taagaaccaa aatgtcttga
     1981 ttgatacagg tgggaaagtg attgatgata ctaaacaacc tagttattca atttctaaat
     2041 atcatatttt accaacgcta aatgaaagag ggataaatga attagagtat ctaattttaa
     2101 cacatccaca caatgaccat attggtgaag tggaatatat tattagtcat attaaaatta
     2161 aacatatagt gatatacaat aagggatata gtagtaatac attgatgtta ttatcgaaat
     2221 taagccataa gtacaacatt aaacttatgg atgtaagaca agttagtagt tttaaacttg
     2281 gagatagtag ttttctattt tttgatagtt ttattccaaa tagccgagat aaaaatgagt
     2341 attcgattat tactatgatt acatatcaaa ataaaaaagt tttattaatg ggcgatgcta
     2401 gtaaaaataa tgaatcttta ctactaaaaa aatataactt gccggagatt gatattttaa
     2461 aagtaggaca tcatgggagc aagacaagta gttctaaaga atttatagag atgattaagc
     2521 ctaaaataag tttgatttct tctgggaaga acaatatgta tcatcttcct aatatagaag
     2581 ttgttaaacg attgcaaagg attcgcagtc gcatttacaa tagtcaacaa aacggtcaag
     2641 ttacaattga cttagatgat aatttaaaag ttgattcaag ctcttatgga aatgcaagtg
     2701 gtttatagat acagaaacat gatataatct attgatgtgt caaacgaatg aaagggtgta
     2761 atgaatgagc gacaatattg tagctattta tggagatgtg cctgaattgg ttgaaaaaca
     2821 aagtgcagaa atcatatcac aatttttgaa aagtgataga gatgacttta actttgtgaa
     2881 atataattta tacgaaacag agattgcacc aattgttgaa gaaacattaa cattgccttt
     2941 cttttcagat aaaaaagcaa ttttggttaa aaatgcatat atatttacag gtgaaaaagc
     3001 gccaaaagat atggctcata atgtagatca attaatagaa tttattgaaa aatatgatgg
     3061 cgaaaatttg attgtctttg agatatatca aaataaactt gatgaaagaa aaaagttaac
     3121 taaaactcta aaaaagcatg caaggcttaa aaaaatagag caaatgtctg aagaagaaat
     3181 aaaaaaatgg attcaaagta aattaaatga gaatttcaaa gatatcaaaa gagatgcatt
     3241 agatttattt attgagttga caggtattaa ctttaatatt gtctcacaag agatagaaaa
     3301 gttgatttta tttttaggcg atagaccaac aattaataag caggatgtta accaaattat
     3361 taatagaagt ttagagcaaa atgtattttt actgactgaa tacattcaga aaagaaagaa
     3421 agaacaagca attcatttag taaaagattt aataactatg aaagaagaac caattaaatt
     3481 acttgcacta attacaagta attaccgatt attttatcaa tgtaagattc tgagtcaaaa
     3541 aggatatagt ggacagcaaa ttgctaaaac aataggagta catccataca gagtaaaatt
     3601 agcgttagga caagtaagac attatcaact tgatgaatta ttgaatatta tagatgcttg
     3661 tgcggaaact gattataaac ttaaatcatc atatatggat aaacagttaa tactggaatt
     3721 attcattcta tctttataaa aaataaaagt tcaagcatgc gcttgaactt ttatttaaaa
     3781 agattattta tttgcagtca ttaattgtga tttaatacgg tcagctttgt ttgaatgtat
     3841 taaattactt tgagcagctt tgtctactaa cttaactgct aagcttacta attcattttt
     3901 attatcagcg ttatttgaaa cagctgtttt agcgttttta actgctgtac gcattgcact
     3961 cttttgtgaa atgttgcgtg cttcagcttt ttcagttgtt tttacacgtt taattgcaga
     4021 tttgatattt gccatttctg tcacctccta aaagtgagtt catcttatca aaattttatt
     4081 tgattacaac aagaaatatt ttaacaaaag tctaaccgtt gtgcaatcat ttatttgatg
     4141 gggttagctt aaaattttgc agatttaata aattgtgttg gaaacacaat caatacgttt
     4201 tataattata agtacgtaat taacatatta acatatcgca agtatgtatt taaataaaga
     4261 ttgttataat ttcaaagttc atccaagatt atggcgtttg catttaccta ttaaaaacgt
     4321 tattatatca aagatgcgaa agataatacg ggtttatttt atgaaagtga gaaggataaa
     4381 atggataatg agcaacgctt aaaaagaaga gagaatataa ggaatttctc gattatagca
     4441 catattgacc acggaaaatc tacattggct gatagaattt tagaaaatac caaatcagtt
     4501 gaaacaagag atatgcaaga tcagttacta gattcaatgg atttagaaag agaacgtggt
     4561 attacaatca aattaaacgc agtacgttta aagtacgaag ctaaagatgg aaatacttat
     4621 acattccatt taatcgatac gcctggacac gtcgatttta catatgaagt gtcacgttct
     4681 ttggcagctt gtgagggcgc gattttagta gtagatgcgg ctcaaggtat cgaagcacaa
     4741 acattagcaa atgtttattt agcattagat aatgagttag agttattgcc tgttattaac
     4801 aaaattgatt tacctgctgc agaacctgaa cgcgtgaaac aagaaattga agatatgata
     4861 ggtttagacc aagacgatgt tgttttagca agtgctaaat ctaacattgg aattgaagag
     4921 atactagaga aaatagttga agttgtgcca gctccagatg gtgacccaga agcaccacta
     4981 aaagcgttaa tatttgattc tgagtatgat ccatatagag gggtaatttc atcgataaga
     5041 attgtggacg gtgttgttaa agccggagat aaaattcgaa tgatggccac tggtaaagag
     5101 ttcgaagtaa cagaagttgg aattaataca cctaagcagc ttccagttga tgaattaaca
     5161 gttggtgatg ttggttatat tattgcaagt attaaaaatg ttgatgattc tagggttggt
     5221 gacaccatca cattagctag tagacctgca tcagaaccat tgcaaggtta taagaaaatg
     5281 aatccaatgg tatattgcgg actgttccca atagataaca aaaattataa tgatttaaga
     5341 gaagcattag aaaaattaca attgaatgat gcatcattag aatttgagcc tgaatcgtca
     5401 caagcattag gttttggtta tagaactggt ttcttaggta tgttacacat ggaaataatt
     5461 caagaaagaa ttgaaagaga atttggtatt gaattaattg caactgcacc atctgtaatt
     5521 tatcaatgtg ttttaaggga cggttcagaa gtgacggttg ataacccagc acaaatgcca
     5581 gatcgtgata aaattgataa aatatttgag ccatatgttc gtgcaactat gatggttcca
     5641 aatgactatg tcggtgcagt aatggaatta tgtcaacgta aacgtggaca atttataaat
     5701 atggactatt tagatgatat tcgtgtaaat attgtttatg aattaccttt agctgaagtt
     5761 gtatttgatt tcttcgatca acttaaatct aatactaaag gatatgcatc atttgattat
     5821 gaattcatcg aaaataaaga aagtaattta gtcaagatgg atattttatt aaatggtgat
     5881 aaagtggatg cgctaagctt catagttcat agagattttg catatgaacg tggtaaagca
     5941 ttagttgaaa aacttaaaac gttaattcca agacagcaat ttgaagtacc tgtacaggct
     6001 gcaataggac aaaaaattgt agcgcgtaca aatattaaat caatgggtaa aaacgtttta
     6061 gctaaatgtt atggcggtga cataagccgt aaacgtaaat tacttgaaaa acaaaaagca
     6121 ggtaaagcta agatgaaagc agttggtaat gttgaaattc cacaagatgc tttcttggct
     6181 gtattgaaaa tggatgatga ataattttaa aaaatcaatt aacaatttac aatgaataaa
     6241 gtttaataac taaaaagagg gagcctagga taaattaacg tcctgggctt tacaatgtta
     6301 tattggcagc catcgacaga gttaaaatga gcttataaca atggggcccc aacacagaag
     6361 ctgacgaaaa gtcagcttac tataatgtgc aagttggggt ggggccccaa catagagaat
     6421 ttcgaaaaga aattctacag gcaatgcaag ttggggtggg acgacgaaat aaattttgcg
     6481 aaaatatcat ttctgtccca ctcccttatg catgagtttt actcatgtaa ttttattttt
     6541 aaggacatat tacatctggc taatgtgtaa gagccactac ataataaatc attagtggtt
     6601 ctttattatt tctatctcac tccctctaaa caagaataaa tattaaaatg aatcgatata
     6661 ttagacaatc attgattaaa cgttaaagtt aaaagtaaga ataattgcag atagtccaac
     6721 aggatatagc cgattggata aaaagtctga gaagcggggc attaaaatga cggtacaaag
     6781 tgcatatata catattccat tttgtgtaag aatatgtaca tattgtgatt tcaataaata
     6841 ttttatacag aatcaacctg tagatgagta cttagatgca ctaatcacag aaatgtctac
     6901 agcaaaatat aggatcttaa agaccatgta tgtaggtggc ggcacaccaa cggccctttc
     6961 tattaatcag ttggaaagat tacttaaagc aatacgtgat acgtttacaa tcacaggcga
     7021 gtatacattt gaagcaaatc ctgatgagtt aactaaagag aaagtccaac tattagagaa
     7081 atatggagta aaaaggattt caatgggcgt tcaaacattc aagccggagt tattgtctgt
     7141 tttaggtaga acgcacaata ctgaagatat ttacacttcg gtgttaaatg ctaaaaacac
     7201 aggtattaaa tcaatcagtt tagatttaat gtatcattta ccgaaacaga cgattgaaga
     7261 ttttgaacaa agtttagatc tagctttaga tatggatatt caacatattt cgagttacgg
     7321 cttaatactt gaacctaaaa cccaatttta taatatgtat agaaaaggct tgctcaaact
     7381 tcctaatgag gatttaggtg ctgacatgta tcagttgctg atgtctaaga tagaacaatc
     7441 tcctttccat caatacgaaa tatctaattt tgcattagat ggccatgaat cagaacataa
     7501 taaggtttac tggtttaatg aggaatatta tggatttgga gcaggtgcaa gtggttatgt
     7561 agatggtgtg cgttatacga atatcaatcc agtgaatcat tatatcaaag ctataaataa
     7621 agaaagtaaa gcaattttag tatcaaataa accttctttg actgagagaa tggaagaaga
     7681 aatgtttctt gggttgcgtt taaatgaagg tgtgagtagt agtaggttca aaaagaagtt
     7741 tgaccaatct attgaaagtg tctttggtca aacaataaat aatttaaaag agaaggaatt
     7801 aattgtagaa aagaacgatg tgattgcact tacaaataga gggaaagtca taggtaatga
     7861 ggtttttgaa gctttcctaa taaatgatta aaaaaattga aatttcgagt ctttaacatt
     7921 gacttacttt gaccaatttg ataaattata attagcactt gagataagtg agtgctaatg
     7981 aggtgaaaac atgattacag ataggcaatt gagtatatta aacgcaattg ttgaggatta
     8041 tgttgatttt ggacaacccg ttggttctaa aacactaatt gagcgacata acttgaatgt
     8101 tagtcctgct acaattagaa atgagatgaa acagcttgaa gatttaaact atatcgagaa
     8161 gacacatagt tcttcagggc gttcgccatc acaattaggt tttaggtatt atgtcaatcg
     8221 tttacttgaa caaacatctc atcaaaaaac aaataaatta agacgattaa atcaattgtt
     8281 agttgagaat caatatgatg tatcatcagc attgacatat tttgcagatg aattatcaaa
     8341 tatatctcaa tatacaactt tagttgttca tcctaatcat aaacaagata ttatcaataa
     8401 tgtacacttg attcgtgcta atcctaattt agttataatg gttatcgtat tttcatcagg
     8461 tcatgttgag catgtacatc ttgcttcaga cataccgttt aataatgaca aactaaatac
     8521 aatatcaaat tttgttacaa acaaactaac tgagtttaat cagaatttac aagatgatat
     8581 tgtctctttt gttcaatcag agcaggaaga aatttttatc aataaattgt taaatacgat
     8641 gaataatcat atttctaatc aaagtaatag catttatatg ggtggaaagg ttaaacttat
     8701 tgatgcattg aatgaaagta atgtatcttc aattcagccc attctacaat atatagaatc
     8761 aaatagaatt gcagaattat tacaagatat atcttcacca aatattaatg taaaaattgg
     8821 taatgaaatt gatgacagct taagtgatat ttcaattgta acaagtcaat atcattttga
     8881 tgaaacgtta aaaggtcaaa tcgctgtgat tggacctaca gctatgcatt atcaaaatgt
     8941 cattcaatta ttaaatagaa tttggtaatg atggattaga aagtattgga ggacagaaaa
     9001 tgacaaataa agacgaatca gttgaaaaaa acactgaatc aacagttgaa gaaacaaacg
     9061 tcaaacaaaa tattgatgat tcagttgaac aagctgaaga aagtaaaggt catttacaag
     9121 atgaagcaat agaagaaacg tctgacgaga atgttattga agaaatagat ccaaaagatc
     9181 aaaaaattaa tgaacttcaa caattagcag atgaaaacga agagaaatat ttaaggctct
     9241 acgctgagtt tgaaaattat aagcgtagaa ttcaaaaaga aaatgaaata aacaaaacat
     9301 atcaagcaca acgtgtgtta acagatattt taccagcaat agacaatata gaacgtgcac
     9361 ttcaaattga aggtgatgat gagactttta aatctcttca aaaaggtgta caaatggtgc
     9421 atgaaagttt gattaacgca ctaaaagata atggtcttga agttattaaa actgaaggtg
     9481 aagcatttga tccaaatatt caccaagctg tagttcaaga tgataaccct gattttgaat
     9541 ctggcgaaat cactcaagaa ctacaaaaag gatacaagct taaagataga gtattaagac
     9601 catcaatggt caaagtaaac caataactta aatttggcga aaagacattg tttaaaatta
     9661 aattaattta atgattaaat ggaggaattt tattatgagt aaaattattg gtatagactt
     9721 aggtacaaca aattcatgtg taacagtatt agaaggcgat gagccaaaag taattcaaaa
     9781 ccctgaaggt tcacgtacaa caccatctgt tgtagctttc aaaaatggag aaactcaagt
     9841 tggtgaagta gcaaaacgtc aagctattac aaacccaaac actgttcaat ctattaaacg
     9901 tcatatgggt actgattata aagtagatat tgaaggtaaa tcatacacac cacaagaaat
     9961 ctcagctatg attttacaaa acttaaaaaa tacagctgaa agctatttag gtgagaaagt
    10021 tgacaaagct gtaattacag tacctgcata ctttaacgat gctgaacgtc aagcaactaa
    10081 agatgctggt aaaattgctg gtttagaagt tgagcgtatc attaatgaac caacagctgc
    10141 agcattagca tatggtttag acaaaactga taaagatgaa aaagttcttg tttttgactt
    10201 aggtggcggt acatttgacg tatctatcct agaattaggt gacggtgtat tcgaagtact
    10261 atcaacagcc ggtgacaaca aacttggcgg tgatgatttt gaccaagtaa ttattgacta
    10321 cctagttgca gaattcaaaa aagaaaatgg cgtagactta tctcaagata aaatggcatt
    10381 acaacgtttg aaagatgctg ctgaaaaagc taaaaaagac ttatcaggtg tatcacaaac
    10441 tcaaatctca ttaccattta tctcagctgg tgaaaacggt ccattacact tagaagtaaa
    10501 cttaactcgt tctaaatttg aagaattatc agattcatta attagaagaa caatggaacc
    10561 tacacgccaa gcaatgaaag acgctggctt aacaaactca gatatcgatg aagttatctt
    10621 agttggtgga tcaactcgta ttccagcagt acaagaagct gtcaaaaaag aaatcggtaa
    10681 agagcctaac aaaggagtaa acccggacga agtagtggca atgggagctg caatccaagg
    10741 tggcgttatc acaggtgacg ttaaagacgt agtattatta gacgtaacac cactatcttt
    10801 aggtattgaa attttaggtg gacgtatgaa tacgttaatt gaacgtaaca ctacgattcc
    10861 tacatctaaa tctcaaatct attcaacagc agtagataat caaccatcag tagatgtaca
    10921 cgtattacaa ggtgaacgtc caatggctgc ggataataaa acacttggta gattccaatt
    10981 gactgatatt ccaccagctg aacgtggtaa acctcaaatt gaagtaacgt ttgatatcga
    11041 taaaaacggt attgtaaatg taactgcaaa agacttaggt acaaataaag aacaaagaat
    11101 tacaattcaa tcaagttctt cattatcaga cgaagaaatc gaccgtatgg taaaagatgc
    11161 tgaagttaac gctgaagcag ataaaaaacg tcgtgaagaa gtagacttaa gaaacgaagc
    11221 tgacagtcta gtattccaag ttgaaaaaac tttaactgat ttaggcgaaa atatcggtga
    11281 agaagataaa aaatctgctg aagagaaaaa agacgctctt aaaactgctt tagaaggtca
    11341 agatatagaa gatattaaat ctaaaaaaga agaacttgaa aaagtgattc aagaattatc
    11401 agcaaaagta tatgagcaag cggctcaaca gcaacaacaa gcacaaggtg ctaatgctgg
    11461 tcaaaacaat gatagtactg tagaagatgc tgaatttaaa gaagtaaaag acgacgacaa
    11521 aaaataatta aacaatacaa ttagaatgtt gtcatgatct gaaaagtcaa agtcaataga
    11581 acattggctt tggcttttta tttggatagt atgacatatt acgttaaact atatgagtta
    11641 attaagaaaa ggagagatag ctgtggccaa aagagattat tatgaagttt taggcattag
    11701 taaggatgct tcaaaagatg aaatcaaaaa agcgtatcga aagctttcga aaaaatatca
    11761 tccagatatt aacaaagaag aaggtgcaga tgaaaagttt aaagaaattt ctgaagccta
    11821 tgaagtttta agtgatgata ataaacgcgc aagctatgat caatttggac atgatggtcc
    11881 acaaggtttt ggtggacaag gattcaatgg ctctgacttt ggcggtttta gtggcttcgg
    11941 tggcggcggt tttgaagata tctttagctc tttcttcggt ggcggcagac aaagagatcc
    12001 aaatgcaccg caaaaaggtg atgatcttca gtatacaatg acattaacat ttgaagaagc
    12061 ggtatttggt acaacaaaag aaatttcaat ccgtaaagat gtaacatgcg aaacatgtca
    12121 tggtgatggt gcaaagcctg gaacaagtaa aaagacttgt agttactgta atggcgctgg
    12181 tcatgtagct gtagaacaaa acacaatttt aggtagagtt cgtactgaac aagtttgtcc
    12241 taaatgtaat ggaagtggtc aagaatttga agaagcttgt ccaacatgtc acggaaaagg
    12301 tactgaaaat aaaacagtta aattagaagt gaaagtacct gaaggtgtcg acaatgaaca
    12361 acaaattaga ttagctggtg aaggctctcc aggcgtgaat ggaggacctg ctggtgattt
    12421 atacgtagta tttagagtta aaccatctga aacatttaaa cgtgatggtg atgatattta
    12481 ttacaaatta aatgttagct tcccacaagc agctttaggc gatgaaataa aaataccaac
    12541 attaaataat gaagttatgt taacaatccc tgctggtaca caaacaggta agcaattccg
    12601 cttaaaagaa aagggtataa aaaatgttca tggctatgga tacggagatt tatatgttga
    12661 tattaaagta gtaacgccta ctaaattaac agatagacaa aaagaactaa tgaaagaatt
    12721 tgcccaatta aatggtgaag aaattaatga acaaccttcg aattttaaag atagagcaaa
    12781 aagattcttt aagggagaat aaagtatgaa ctggacagag ctttcaatta ttattaatca
    12841 tgaagcagta gaattggcta ccaatatact tgaaaatcat ggatcaaatg gtgtcgtgat
    12901 agaagattca gatgatttaa ttaaccaacc agaagataaa tacggtgaaa tttacgcttt
    12961 gaaaaaagag gattatccag ataagggagt aagattgaaa gcctatttta atgaaatgac
    13021 ttatgatgat aagttgcgac agcaaattaa agatgagtta ttaaatttag atgaacttga
    13081 tcaacataac gttcaattca gtgagcaaat tattgcagag acggattggg aaaatgaatg
    13141 gaaaaactat ttccatccat tccgagcgtc gaagaagttc acaatagttc ctagttggga
    13201 aacatatgct aaagaagcgg atgaagagct ttgcattgag ctcgacccag gtatggcttt
    13261 tggaacaggt gatcatccga ctacaagtat gtgtttgaag gcaatagaaa catatgtatt
    13321 gccacagcat tcagtaattg atgttggtac tggctcaggt atattaagta ttgcaagtca
    13381 tctaatcggt gtaaaacgta ttaaagcgtt ggatattgat gaaatggcag tgagtgtagc
    13441 taaagaaaac ttcagaagaa atcattgtga aacgttaatt gaagctgttc caggtaactt
    13501 attgaaagac gaaacagaaa aatttgatat tgtaatagca aatattttag cgcatattat
    13561 tgatgaaatg attgaagatg cttataatac tctaaatgaa ggcggttatt ttattacttc
    13621 tggtattata aaagagaagt atgaaggtat acagtcacat atggagcgtg taggttttaa
    13681 aattatttca gaacaacatg acaacggttg ggtttgtctt gttggccaga aagtgagtga
    13741 ataatgtgca acgttatttc atagaccaaa acgctgatgt aagtcagcgt ttttttatta
    13801 caaaaaaaga agatattcat catataacaa atgtaatgag atatgatgtt ggtagtaaaa
    13861 ttattttaac ttttaatgat caaacagttt atttatgtga aattgctgac ataacaagtg
    13921 ataaaattga agttgattta aaagaaaaac aaaatattaa tacagaattg ccagttgatg
    13981 ttacgatttg cagtggacta atcaaagctg acaaatatga gtggttgcta caaaaagcta
    14041 ctgaattggg tgcttcatca tttatagctg tgagcatgga acgttcaatt gttaaattaa
    14101 acgaggcaaa agttgccaaa aagatagagc gttggcaaaa aataattaaa gaagctgctg
    14161 aacaaagcta tcgtttagtg attccatcta ttcaattcga gtcgaattta aaattaattt
    14221 gtgatactat tgataattat gactatattc ttattgcata tgaagaggaa gcaaaagatg
    14281 gtgagttaag caatttcaag caaactttac aacaattcaa tgctcaggat aaagtgttga
    14341 tgatttttgg tcctgagggt ggtttgtcag aaaatgaaat ttcattattt agtaatacta
    14401 gtacagttgt tggtcttggg ccgagaattt tacgggcaga aactgcgcca ttgtatgcgt
    14461 taagtgcaat aagttatgaa aaagaattaa tggggtgaat atttatgtca acagttgcgt
    14521 ttcacacatt aggttgtaaa gtaaaccatt atgaaactga agcaatctgg caattattta
    14581 aagaagcaaa ctatgagcgc gttgactttg aagcgaatgc tgatgtattt gttattaata
    14641 cttgtacagt aacgaatacg ggtgataaaa aaagtcgtca aataattaga cgtgcaataa
    14701 gacaaaatcc tgatgctgta atctgtgtaa caggttgtta tgcgcaaact tcatcagctg
    14761 aaattatgga aattcctggt gtcgatgtag tagttggtac acaagataga cataaactat
    14821 taggttacat tgacgaattc cgtaaagaac gccaaccaat taatggtgtt ggaaatatca
    14881 tgaaaaatcg taaatatgaa gaattagatg tcccttattt tacagataga acacgtgcgt
    14941 cattaaaaat tcaagaaggt tgtaacaact tctgcacatt ctgtattatt ccatgggctc
    15001 gtggcttaat gcgttcaaga gatccggaaa aagtagttga acaagcgacg caactagtga
    15061 attcaggata taaggaaatt gtattgacgg gaattcatac aggtggatat ggtcaagatt
    15121 taaaagatta taacttggcc caattattac gtgatcttga aacgattaat ggattagaac
    15181 gaattcgaat ttcttcaatt gaagcaagtc aacttacaga tgaagtaatt gacgttttag
    15241 aacgttcaac aaaagttgtg cgtcatttgc atattccatt acaatctggt tcagatacag
    15301 tattaaaacg tatgagacgt aagtatacaa tggatagatt ttcagaacga ttaacaaaat
    15361 tgcataaagc tttaccagac ttggcagtta cgagtgatgt aattgttggt ttcccaggtg
    15421 aaactgaagc tgagttccaa gaaacatatg attttatcgt aaagcataag ttctctgaac
    15481 tgcatgtttt cccttattct cctagaattg gcacgccagc tgcaagaatg gatgaccaaa
    15541 ttgatgaaga aattaaaaat gaacgggtgc ataagttaat tacgctaagc aatcaactcg
    15601 gaaagttata tgcgtctaaa tttgatcaag atgtgcttga agtaattcct gaggaacagg
    15661 gtgacacaga aggtacatta gttggatatg cagataatta tatgaaagta caatttgaag
    15721 gtgacgaatc actcataggt caaattgtaa aagttaaaat tacgcaagca aattatccat
    15781 taaatgaagg gcaagcaatt aaagttgttg atttcgcaac aaataaatct gatagagaag
    15841 ttttagttta agtattcctt gattcatacc atatggttcc gtattaaaga tataggtttt
    15901 gtaacgttgt tttaacggct tcacaattta ctatataagc aactcttctc attaaataaa
    15961 ttattaagag atttttagaa caaaatctaa aagtctttgt gattttgatg tgaaatatgt
    16021 ttcaaataaa aaacaattta ctaattgacc ataaattaca gatatattat acttataaat
    16081 gcatagtttt actgtgcaat tgactataaa gttccgttga tatttggagg gagggaaata
    16141 cagatgtcta aaacagtagt acgtaaaaat gaatcacttg aagatgcgtt acgtagattt
    16201 aaacgttcag tttctaaaag tggaacaatc caagaagtac gtaaacgtga attttacgaa
    16261 aaaccaagcg taaaacgtaa aaagaaatca gaagctgcac gtaaacgtaa attcaaataa
    16321 ttaatacctc tgttgactcc ctcaacacga atattaatta tataaaacaa a
//
LOCUS       DAKTGS010000055        16303 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.055, whole genome shotgun sequence.
ACCESSION   DAKTGS010000055 DAKTGS010000000
VERSION     DAKTGS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.055"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            307..1875
                     /locus_tag="O0286_001446"
     CDS             307..1875
                     /locus_tag="O0286_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0169829.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            2017..2775
                     /locus_tag="O0286_001447"
     CDS             2017..2775
                     /locus_tag="O0286_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0169830.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
     gene            2969..3478
                     /locus_tag="O0286_001448"
     CDS             2969..3478
                     /locus_tag="O0286_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCY0169831.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(3591..4781)
                     /locus_tag="O0286_001449"
     CDS             complement(3591..4781)
                     /locus_tag="O0286_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0169832.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(4759..5934)
                     /locus_tag="O0286_001450"
     CDS             complement(4759..5934)
                     /locus_tag="O0286_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCY0169833.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            6367..7848
                     /locus_tag="O0286_001451"
     CDS             6367..7848
                     /locus_tag="O0286_001451"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCY0169834.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            7832..8092
                     /locus_tag="O0286_001452"
     CDS             7832..8092
                     /locus_tag="O0286_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCY0169835.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            8092..9654
                     /locus_tag="O0286_001453"
     CDS             8092..9654
                     /locus_tag="O0286_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCY0169836.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            9956..10759
                     /locus_tag="O0286_001454"
     CDS             9956..10759
                     /locus_tag="O0286_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCY0169837.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            10978..13302
                     /locus_tag="O0286_001455"
     CDS             10978..13302
                     /locus_tag="O0286_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0169838.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            13319..14677
                     /locus_tag="O0286_001456"
     CDS             13319..14677
                     /locus_tag="O0286_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0169839.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            14816..>16303
                     /locus_tag="O0286_001457"
     CDS             14816..>16303
                     /locus_tag="O0286_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0169840.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSD"
BASE COUNT         5705 a         2248 c         3039 g         5311 t
ORIGIN      
        1 ttatttagta tatcctaaat agcagtgctt aaaaaggtta atatttttta aatatactta
       61 aattaaccat tcactttcta ttttaagata tttactttca atagtttgtg agacttttca
      121 atgactcgta agtctgcaat tggatcaaac agcaccagtt tatatgaata acttatttta
      181 atattaaacg tgtttaaata gtgaaatgat catattaata tttataagaa tattctgaaa
      241 aatatattga agtgtctcgt gcttaatcgt ataatatttt tatgtttaaa caataggggg
      301 aatcttatga ttgaaaaatt agtaaccttt ttaaatgagg ttgtttggag taagccatta
      361 gtttatggtt tgctaattac tggtgtgcta tttacattgc gtatgcgatt ttttcaagtt
      421 agacatttta aagaaatgat tcgattaatg tttcaaggag agaagtctcc taatggtatt
      481 tcaagttttc aagcgatagc catgtcttta gcaggcaggg ttggtacagg taatattgtc
      541 ggtgtatcta ctgcaatatt tataggagga cctggtgcag tattttggat gtggattact
      601 gcgtttttag gtgcaagtag tgcttttatt gaatctacac ttggtcaaat attcaagaga
      661 gttgaaaata atgaataccg tggtggacca gcgtattata ttgaatatgg tattggtggt
      721 aaatttggta aaatttacgg aattatcttt gctattgtta cgattatctc agtaggtcta
      781 ttgcttcctg gtgtgcaatc taacgctata gcaagttcta tgcataatgc gattcatgtt
      841 ccacaatggt taatgggtgg tattgttgta gttattttgg gattaattat ttttggtggt
      901 gtacgtagta ttgccaatgt tgcaacagcc gttgtaccat ttatggcaat tatttacata
      961 ctgatggctg tcattatcat ttgtatcaat atacaagaag tgccagcgtt atttgcatta
     1021 attttcaaat cagcatttgg attacaatct gcttttggtg gtatcgttgg cgcaatgata
     1081 gagattggtg ttaaacgtgg attatattca aatgaggctg gtcaaggtac aggtccacac
     1141 gcagcagcgg cagcagaagt atcacatcca agtaaacaag gtctagtaca agcattttca
     1201 gtttatattg atacattatt tgtatgtact gcaactgctc tgattatact tatttctggt
     1261 acatataatg tgactgatgg tacggttaat gcgaatggca caccgcattt aattaaagat
     1321 ggcggtattt atgttgaaaa tgcaacaggt aaagattatt caggtactgc gatgtatgca
     1381 caagccggca ttgataaagc gttccatggc agtggttatc aatttgatcc tactttctct
     1441 ggcgtaggtt cgtactttat tgcatttgct ttattcttct ttgcatttac tacaattttg
     1501 tcgtactact acattacaga aacaaatgtt gcttatttaa cgcgtaatca aaataatcaa
     1561 gtttcatcga tatttattaa tattgctcgt gtgattattt tgttcgctac attttacggt
     1621 gcagttaaaa cagctgatgt agcatgggca ttcggtgatt taggtgtagg tctaatggct
     1681 tggttaaata tcattgcgat ttggatttta cataagcctg ccgtaaatgc tttaaaagat
     1741 tatgaaattc aaaagaaacg tttaggcaac ggttataatg cagtttatca acctgatccg
     1801 aataaattac ctaatgctgt cttttggttg aagacgtatc cagaacgttt aaaacaagca
     1861 cgtgccaaaa agtaatctac ttttgtttat agtatatgta gtgatcattt gataaaaaag
     1921 aaaagtattg agaattttag gtgctcagaa atttgaattt taaaaatata gtgtctcttg
     1981 gtacaataac aatacaacta ctaggggcac ttttttatgt cagaatttaa aactggtaag
     2041 attaataaac atgttttata tagtaatatt ttaaatagag atgtcacgtt aagtatttat
     2101 ttaccagaat cttataatca acttgttaaa tataatgtca ttctttgctt tgacggatta
     2161 gattttttac gtttcgggag aatacaacgt acatatgaat cgttaatcaa agaagcgcgt
     2221 attgatgatg cgatcattgt tggattccat tatgaagacg ttgataagcg tagagaggaa
     2281 tttcatccac aaggaagtcg ttctcattta actattcaat cagtcggtaa agaaatattg
     2341 ccatttattg actcgacgtt ttctacactg aaagtaggta atgcaaggtt attagtaggg
     2401 gatagtttag cgggtagtat tgccttatta acggcgttga cctatccaac gatttttagt
     2461 cgtgtagcaa tgttaagtcc acattcagat gaaaaagtat tagataagct aaatcaatgt
     2521 gcaaataaag aacaattgac aatttggcat gtcattggtc tagatgaaaa agattttact
     2581 ttaccaacaa atggtaagcg tgccgatttc ttaacaccga atagagaatt agctgaacaa
     2641 attaagaaat ataatataac ttattattac gatgaatttg atggtggtca ccaatggaaa
     2701 gattggaaac cattgctgtc agatatatta ttgtattttt taagtaaaaa cacagatgat
     2761 caactttatg aataatttac attagtagat ttagtatgaa ttgtcttcat atagtctggt
     2821 ctataatata atttataaaa gattttactg tttaatttaa tttaaatttg acgaaattgc
     2881 aaaagatgta taatgaatta tttttaatgt aacggttttc aaagaaattt gatataatag
     2941 caataggtta aacaaaggag gaattcagat gattttagga ttagcattaa ttccatcaaa
     3001 gtcatttcaa gaagcggtgg attcttaccg taaaagatat gataaacagt attcacgaat
     3061 taaaccacat gtgacaatta aagcgccatt tgaaattaaa gatggtgatt tagattctgt
     3121 cattgaacag gttagagctc gtattaatgg tataccagca gtagaagttc atgctacaaa
     3181 agcttctagc ttcaaaccaa cgaacaatgt gatttacttt aaagttgcga agacggacga
     3241 cttagaagaa ttgtttaatc gctttaatgg agaagatttc tatggagaag ctgaacatgt
     3301 ttttgtgcca cactttacaa tagcacaagg actatctagc caagaattcg aagatatttt
     3361 tggtcaagta gcattagctg gggtagacca taaagaaatt atcgatgaat taactttgtt
     3421 acgttttgac gatgacgaag ataaatggaa agttattgaa acgtttaaat tagcttaagt
     3481 aacataatag tattgttaat cgtagtatgt ttgaattaat aagaaaatgg tcatttttat
     3541 tgaatgtaat aaaaatgacc attttcttta ttttaaaata cgttttaacc ttacttagct
     3601 ttttctctat ttactataaa gtagcttcca taaaatacag ctaagactaa aaagattaat
     3661 gccgagaaat aaaatgtatt gtttaaattg ttggtaaatt gtgtaattaa tccgccaaat
     3721 aatggcccta tcattgagcc gaatccttgg atactattaa aaacacccca agtttcttct
     3781 tgttcatctg atttgataaa tcgtgccata aaggtattcc atgctggtaa taagatgcca
     3841 tacattagac cgatagctaa agcgataatc cacaagatgt gaatattaac aatcatagat
     3901 agagtaaaaa ttaatatcat gtataaaata aatccgctta gaataacacc atacataaag
     3961 tttctgctgc ggttatctat tagtttcgat aaaaatagca tcgaaactgc acagccgata
     4021 ccaccaataa tgattgcaac agtatattca attgtgctta cgttaataac cttagtagca
     4081 tatgttggta atataggaac tagggcagca attgcggcac cttgtaaaag aataccaggg
     4141 aacaacaata aatggcgctt tgtcacatca acaatttgtc tcaattgagc tttaactgga
     4201 cgagtattat aatttgttaa ctttacatcg acaaaataat ataatatcca tgcaattaaa
     4261 acgactaaag acatcatgaa ggcaaagcgt gttgggtgca ctttgataag tagattcata
     4321 aaaaccatac ctaccaatag gcctaacaac catgaaaaat aaacatagcc catttgtttg
     4381 ccacgtttat cttcttcaac actggataac ataatgaccc aaataggact aactgcaata
     4441 ccgagcatca tagcactaaa tatgattaca aaaggtgatg ctggaaacca aataactaaa
     4501 aataaacttg taaatgctaa aataaatcca gtcgttaaaa cgatttttgt gccgaatttt
     4561 ttcagtaaaa atcctataac aaagtttgta gatgcatcag caataaaatg tattgaaaat
     4621 gctagagacg ttattgctac agcaatggat gtaactgttg gcaagaaatt aatatagctt
     4681 aggatataca tgcctctcgc aaattccatt aaaaataaga taataagcat taaaatgaaa
     4741 tttttatgat tagcgtaatt atttaacgaa gaatcttgca tataaaggaa cctttccata
     4801 aatctcttgt ggttgtgatg aatgaccgat taaatcaagt aagtctcgac atattgtctg
     4861 tgtagcatac ttaattttat cttgttccat tgtactaatc atgttagtta attgctcatt
     4921 accgttagtt aaacttgcta caatttttat tgcttcttct ggagtatcag cgattttacc
     4981 aaaacctttt tcttcaaagt aaagggcatt ttcaagctct tgaccaggtg caggatttag
     5041 gaaaatcatt ggaatacaac gggcgaaacc ttcagttatt gtgataccac caggtttcgt
     5101 aatcataagt tgacttgatg ccatccattc attcatgtgt ttggtataac ctagaatcaa
     5161 tacattctcg ttagatttaa acttagctgt taaagaacgc tttagctctt tgctcttacc
     5221 acaaatcata actacttgtg catttgcact tttcgctaat atatcagtaa tcatcgtgtc
     5281 aaaaccttta gatacaccaa atgcaccagc tgacattaaa atagtttgct tatctggatc
     5341 taagttgttg tctattaacc actgcttttg attaataggc gtttcaaatt tgttatcaat
     5401 aggaatacct gtcactttaa ctgttgaagg atcaatacct acgtctatga agtcttgttt
     5461 cgtttctttt gttgccacat aatatcttgt tgaatacggc gtaatccagt ttttatgtaa
     5521 gcgatagtct gtcatcactg tagcaactgg aatattaatg ttaaattgct cagttagtac
     5581 cgacataact ggtgtaggaa acgttaataa tattaaatct ggcttttctt ttatcaataa
     5641 attaattaac ttattaagtc catagtattt gtaaaaacat ttgtctagtt tatctgggcg
     5701 gctgtaataa aaccctttgt acatatttct aaaatattta aagctattga tataccattt
     5761 tttacaaata gaagtcaaaa ttggatgagc ttccataaat aaatcgtgct caatgacgct
     5821 taaatggtct agattcatat cattaagttg attaacgata ctctgtgtaa cttgcatatg
     5881 accgttaccg aatgagccag taataatcaa tatcttttta ttttgagtaa ccattaatag
     5941 ccaccctccg ttagtttgaa aattttattt aagtgtaact tattttacgg cattataaaa
     6001 gaaataaaga cgcaaagtcg ttacatttat agcaatttta atctatagat gaattgatac
     6061 aaaataaaac gttattttat aaagcaattt attgttctat gttttatttg tatatttaaa
     6121 attatccagt atacaattat agcatatttt tggaaacaat tatgatatta taccatgtta
     6181 caagatggtt ttaataattt aagatgagcc ataattgtaa aactaattca taataccgta
     6241 tgttttattt ttaatagtag aaattagaaa atgctgatta gtaggatata acagtgaaat
     6301 tataaattta ttaacatcaa caaaacgtgt ataataaaca tattgtagaa aaaggagcgg
     6361 ttcagtttgg atgcaagtac gttgtttaag aaagtaaaag taaagcgtgt attgggttct
     6421 ttagaacaac aaatagatga tatcactact gattcacgta cagcgagaga aggtagcatt
     6481 tttgtcgctt cagttggata tactgtagac agtcataagt tctgtcaaaa tgtagctgat
     6541 caagggtgta agttggtagt ggtcaataaa gaacaatcat taccagctaa cgtaacacaa
     6601 gtggttgtgc cggacacatt aagagtagct agtattctag cacacacatt atatgattat
     6661 ccgagtcatc agttagtgac atttggtgta acgggtacaa atggtaaaac ttctattgcg
     6721 acgatgattc atttaattca aagaaagtta caaaaaaata gtgcatattt aggaactaat
     6781 ggtttccaaa ttaatgaaac aaagacaaaa ggtgcaaata cgacaccaga aacagtttct
     6841 ttaactaaga aaattaaaga agcagttgat gcaggcgctg aatctatgac attagaagta
     6901 tcaagccatg gcttagtatt aggacgactg cgaggcgttg aatttgacgt tgcaatattt
     6961 tcaaatttaa cacaagacca tttagatttt catggcacaa tggaagcata cggacacgcg
     7021 aagtctttat tgtttagtca attaggtgaa gatttgtcga aagaaaagta tgtcgtgtta
     7081 aacaatgacg attcattttc tgagtattta agaacagtga cgccttatga agtatttagt
     7141 tatggaattg atgaggaagc ccaatttatg gctaaaaata ttcaagaatc tttacaaggt
     7201 gtcagctttg attttgtaac gccttttgga acttacccag taaaatcgcc ttatgttggt
     7261 aagtttaata tttctaatat tatggcggca atgattgcgg tgtggagtaa aggtacatct
     7321 ttagaaacga ttattaaagc tgttgaaaat ttagaacctg ttgaagggcg attagaagtt
     7381 ttagatcctt cgttacctat tgatttaatt atcgattatg cacatacagc tgatggtatg
     7441 aacaaattaa tcgatgcagt acagcctttt gtaaagcaaa agttgatatt tttagttggt
     7501 atggcaggcg aacgtgattt aactaaaacg cctgaaatgg ggcgagttgc ctgtcgtgca
     7561 gattatgtca ttttcacacc ggataatccg gcaaatgatg acccgaaaat gttaacggca
     7621 gaattagcca aaggtgcaac acatcaaaac tatattgaat ttgatgatcg tgcagaaggg
     7681 ataaaacatg caattgacat agctgagcct ggggatactg tcgttttagc atcaaaagga
     7741 agagaaccat atcaaatcat gccagggcat attaaggtgc cacatcgaga tgatttaatt
     7801 ggccttgaag cagcttacaa aaagttcggt ggtggccctg ttgattaata aaagatttat
     7861 tgatgaaggt aaaactattg atgtttattt attcgaagca ttaaataacc agataatcat
     7921 tgctatacca gattggtttt ggtcatatca gatggcaatg acattagatg aagaaacttg
     7981 ttttgaagca atactcatgc aattgtttgt ttttaaagaa gaggaagagg cagaatcgat
     8041 tgcatcacaa ctaacagatt ggatagaaac atataaaaag gagaaagact aatgaactta
     8101 aagcaagaag ttgagtctag aaagactttt gcgattattt cacatcccga tgcagggaaa
     8161 acaacgttaa ctgaaaaact attgtacttc agtggtgcta ttcgtgaagc gggtacagtt
     8221 aaagggaaga agactggtaa atttgcgaca agtgactgga tgaaagttga acaagagcgt
     8281 ggtatttctg taactagttc agtaatgcaa tttgattacg atgattataa aatcaatatc
     8341 ttagatacac caggacatga agacttttca gaagatacgt atagaacatt aatggcagtt
     8401 gacagtgctg tcatggtcat agactgtgca aaaggtattg aaccacaaac attgaagtta
     8461 tttaaagttt gtaaaatgcg tggtattcca atctttacat tcattaataa attagaccga
     8521 gtaggtaaag aaccatttga attattagat gaaatcgaag agacattaaa tattgaaaca
     8581 taccctatga attggccaat tggtatggga caaagtttct ttggcatcat tgatagaaag
     8641 tctaaaacaa ttgaaccatt tagagatgaa gaaaatatat tacatttgaa tgatgatttt
     8701 gagttggaag aagatcatgc aattacaaat gatagtgatt ttgaacaagc gattgaagaa
     8761 ttaatgttgg ttgaagaagc gggtgaagcc tttgataatg acgcgctgtt gagtggagac
     8821 ttaacacctg tatttttcgg ttcagcttta gctaactttg gtgtacaaaa tttcttaaat
     8881 gcatatgttg attttgcgcc aatgccaaat gcgagacaaa caaaagaaga cgttgaagta
     8941 agcccgtttg atgattcatt ttcaggattt atctttaaaa ttcaagccaa catggaccct
     9001 aaacaccgtg atagaattgc ctttatgcgt gtcgttagtg gtgcatttga acgtggtatg
     9061 gatgttactt tgcaacgtac taataaaaag caaaagatca cacgttcaac gtcatttatg
     9121 gcagacgata aagaaactgt gaatcatgct gtagcaggcg atatcattgg actatatgat
     9181 actggtaatt atcaaattgg agatacttta gttggtggaa aacaaaccta cagtttccaa
     9241 gatttaccac aatttacgcc agaaattttt atgaaagttt ctgctaaaaa cgtcatgaaa
     9301 cagaagcatt tccataaagg tattgaacaa ttagtacaag aaggtgcgat tcaatactat
     9361 aaaacattac acacaaacca aattatttta ggtgctgttg gtcagttaca atttgaagtt
     9421 ttcgaacata gaatgaaaaa cgaatataat gttgatgttg ttatggagcc agtaggccgt
     9481 aaaattgcac gttggattga aaatgaagac caaattacag ataagatgaa cacatcaaga
     9541 tcgattttag tgaaagatag atatgacgat ttagtattct tatttgaaaa tgaatttgca
     9601 acaagatggt ttgaagagaa attccctgaa attaaattgt atagtttact ttaacagctc
     9661 aattgtataa tcgaatttgt tacattaaaa ataattgttt cgttgaagaa aaataaattg
     9721 tatattttaa aagaaaaagg tatactatga tgtatcaaat gaataaccta tggcattttg
     9781 tcagagggga gtaacttaag aatcatgacc gtataaatga ttcgacactt tatcgtcatt
     9841 acgaagatat cttccggtaa agtgggcaat ttaaattgct tagtgagacc tttgctattt
     9901 atttagcata ggtctttttg tttgtactta acttatttat ttaaaggagt tgtacatgtt
     9961 aatggatcca agtttgatct taccttattt atgggtactt gtcgttttag tatttttaga
    10021 aggcttatta gcagcagata acgcgattgt tatggctgta atggttaagc acttaccacc
    10081 cgaacaacgt aaaaaagctt tgttttacgg tttgttaggt gcatttgtat ttagattttt
    10141 agcattattc ttaattagta ttatcgcgaa cttttggttt attcaagctg caggagcggt
    10201 ttacttaatt tatatgtcaa tcaaaaatct gtggcagttc tttaaacacc cagaaattga
    10261 aagtcctgaa gctggagatg atcatcatta tgatgaatct ggtgaagaga ttaaagcaag
    10321 taacaaatca ttctggggaa ctgtgttgaa aatagaattt gcagatatcg catttgccat
    10381 tgattctatg cttgctgctt tagctattgc tgtaacactt cctaaagttg gtattcactt
    10441 tggtggtatg gacttaggtc agttcgtagt catgttccta ggtggaatga ttggtgttat
    10501 tctaatgcgt tatgcagcaa catggtttgt agagctatta aacaaatatc caggacttga
    10561 aggtgcagcc ttcgcgatcg ttggttgggt aggtgttaaa ttagttgtca tggtattagc
    10621 gcacccagac atcgctgtat tgcctgagca cttcccacat ggcgtattat ggcaatctat
    10681 tttctggaca gtactaattg gattagtaat tatcggttgg ttaggttcag ttgttaaaaa
    10741 taaaaaatcg cataaataat tgatgtgaag cggacaatct taatttagtt taaggttgtc
    10801 ctttttcatt taattgagtg atttatgaaa aatggatttt gaagaatgtg aatcaaaaga
    10861 tgcgatatag tattaagaaa atgtgccttt tatatttagc attttttcaa tagaaattat
    10921 atagatttta aagcaaatta ggtgttaatg tgtcataatg ataagtgatt ttattgaatg
    10981 gagtggacat tagtggatat tggtaaaaaa catgtaattc ctaaaagtca gtaccgacgt
    11041 aagcgtcgtg aattcttcca caacgaagac agagaagaaa atttaaatca acatcaagat
    11101 aaacaaaata tagataatac aacatcaaaa aaagcagata agcaaataca taaagattca
    11161 attgataagc acgaacgttt taaaaatagt ttatcatcgc atttagaaca gagaaaccgt
    11221 gatgttaatg agaataaagc tgaagaaagt aaaagtaatc aggatagtaa gtcagcatat
    11281 aacagagatc attatttaac agacgatgta tctaaaaaac aaaattcatt agattcagtg
    11341 gaccaagata cagtgaaatc aaaatattat gagcaaaatt ctgaagcgac tttatcaact
    11401 aaatcaaccg ataaagtaga atcaactgaa atgagaaagc taagttcaga taaaaacaaa
    11461 gttggtcatg aagagcaaca tgtactttct aaaccttcag aacatgataa agagactaga
    11521 attgattctg agtcttcaag aactgattca gacagctcga tgcagacaga gaaaataaaa
    11581 aaagacagtt cagatggaaa taaaagtagt aatctgaaat ctgaagtaat atcagacaaa
    11641 tcaaatacag taccaaaatt gtcggaatct gatgatgaag taaataatca gaagccatta
    11701 actttaccgg aagaacagaa attgaaaaga cagcaaagtc aaaatgagca aacaaaaacc
    11761 tatacatatg gtgatagcga acaaaatgac aagtctaatc atgaaaatga tttaagtcat
    11821 catataccat cgataagtga tgataaagat aacgtcatga gagaaaatca tattgttgac
    11881 gataatcctg ataatgatat caatacacca tcattatcaa aaacagatga cgatcgaaaa
    11941 cttgatgaaa aaattcatgt tgaagataaa cataaacaaa atgcagactc gtctgaaacg
    12001 gtgggatatc aaagtcagtc aactgcatct catcgtagca ctgaaaaaag aaatatttct
    12061 attaatgacc atgataaatt aaacggtcaa aaaacaaata caaagacatc ggcaaataat
    12121 aatcaaaaaa aggctacatc aaaattgaac aaagggcgcg ctacgaataa taattatagt
    12181 gacattttga aaaagttttg gatgatgtat tggcctaaat tagttattct aatgggtatt
    12241 attattctaa ttgttatttt gaatgccatt tttaataatg tgaacaaaaa tgatcgcatg
    12301 aatgataata atgatgcaga tgctcaaaaa tatacgacaa cgatgaaaaa tgccaataac
    12361 acagttaaat cggtcgttac agttgaaaat gaaacatcaa aagattcatc attacctaaa
    12421 gataaagcat ctcaagacga agtgggatca ggtgttgtat ataaaaaatc tggagatacg
    12481 ttatatattg ttacgaatgc acacgttgtc ggtgataaag aaaatcaaaa aataactttc
    12541 tcgaataata aaagtgttgt tgggaaagtg cttggtaaag ataaatggtc agatttagct
    12601 gttgttaaag caacttcttc agacagttca gtgaaagaga tagctattgg agattcaaat
    12661 aatttagtgt taggagagcc aatattagtc gtaggtaatc cacttggtgt agactttaaa
    12721 ggcactgtga cagaaggtat tatttcaggt ctgaacagaa atgttcctat tgatttcgat
    12781 aaagataata aatatgatat gttgatgaaa gctttccaaa ttgatgcatc agtaaatcca
    12841 ggtaactcgg gtggtgctgt cgtcaataga gaaggaaaat taataggtgt agttgcagct
    12901 aaaattagta tgccaaacgt tgaaaatatg tcatttgcaa tacctgttaa tgaagtacaa
    12961 aagattgtaa aagatttaga aacaaaaggt aaaattgact atcccgatgt aggtgttaaa
    13021 atgaagaata ttgccagtct aaatagtttt gaaagacaag cagttaaatt gccaggaaaa
    13081 gttaagaacg gtgttgttgt agatcaagtt gacaacaatg gtttagcaga tcaatctggt
    13141 ctgaaaaaag gtgatgtaat tactgaatta gatggcaaac ttttagaaga tgatttacgc
    13201 tttaggcaga ttatatttag tcataaagat gacttgaaat caattacagc gaagatttat
    13261 agagatggta aagagaaaga aattaatatt aaactaaaat aatgttgagg tgaacaacgt
    13321 gtcaattttt agccagtttt taaaaagatc aagccctcaa caaggtattg tattgtacta
    13381 tatcgtcgca attgtcattg catttttatt attaaactta ccgtatgttc ataaaccagg
    13441 tgtagaagta aatccaattg acacattatt tgttgccgta tccggaatta gtgttactgg
    13501 attgtctccg ataagtattg tcgataccta ttctacattt ggacaattaa ttatcctcgt
    13561 gatattaaat attggtggaa ttggcgtcat ggcaattggt acgatgttat gggtggtact
    13621 aggtaaacat attggaatta gagaacgtca gttaattatg ttagataata acaaaaacac
    13681 aatgagtggt accgtcaaat tgattattga tattgtaaaa tcaatatttg taatcgaact
    13741 cgtaggagcc atgttattag cattttactt ttatcgagat aatccagatt taaaatatgc
    13801 aatcatgcaa ggtgtttttg tttctatttc tgccactacc aatggtggat tagatattac
    13861 aggtaagtca ttaattcctt atgcacatga ttattttgta caagcgatag ttatattttt
    13921 aataatttta ggatcaatcg gcttcccagt attattagaa gttaaagctt atattcaaaa
    13981 tagggttact aattttagat tttcattatt tactaaaatt acgacatcaa catatttatt
    14041 cctatttatt gttggggtat tagccattct attatttgaa cataaccatg cgttcaaagg
    14101 tttaagttgg catcaatcgt tattctattc gctgtttcaa tcagcgacta caagaagtgc
    14161 gggtcttcaa acaattgatg tgacaacact aagtgacccc actaatatta tcatgggtat
    14221 tttaatgttt ataggatctt cgccaagttc ggttggtggc ggtattcgta caacaacttt
    14281 cgctatttta attttgtttt taattaactt tagtaataat gccgataaaa catccattaa
    14341 agtttacaat agagaagtac acattatgga tattcaacgt tcatttgcag tatttacaat
    14401 ggcgacaatt ttaacatttt taggaatgct aattatatca gctactgaaa atggtaagct
    14461 tacattttta caagtatttt ttgaagtcat gtctgcattt ggaacttgtg gactatcgct
    14521 tggtgtcaca agtgatatta gtgatatttc taaggtcgta ctaatgatac tcatgtttat
    14581 aggacgtgtt ggcttaatat catttatcat tatgatagca ggacgtcgag aaccagataa
    14641 attccattat ccaaaagaac gtattcaaat aggataatat aatagcaatc taagtttagt
    14701 taatgtagat tttaactgga acttagattg cttttttagt ttgtattttt aacttatttt
    14761 ataagacgat tggtttcgaa aatggtaaac tagtaacaat gagaggtgta acatgatgga
    14821 aaaaaatgaa aacattaatg tagagatttt aactacgtca gatatgcata gtcatttctt
    14881 aaatggtgat tatggttcaa atatttatag agctggtact tatgttaacc aagtaagagc
    14941 acaaaatcat cgcgtcattt tattagatag tggcggaagt ttagctggct cgttagcggc
    15001 ctattattat gctattgttg caccttataa acgacatcca atgataaagt taatgaacag
    15061 aatgcattat gatgctagcg gtgtgagtcc aagtgaattc aagtttggtt tatcattttt
    15121 aactcgttca attgctttgg cacgttttcc atggttatca gcaaatattg aatacaatgt
    15181 tactaaggag ccttattttt caactccata ttgtattaaa cattttggtg acttaaaaat
    15241 tgctatcgta ggcgtcacag cagatggttt aatggaaaat gagtattctg aaatggagca
    15301 agatgtatct attgaaaaga cattagtggc atcaaaacgt tggattagat atatccatga
    15361 agttgaagag ccagattttt tgattgtaat ttatcatggt ggattgaata aaattagtaa
    15421 tagtacgaaa aataaaaagg caagttcgaa tgaagctgaa aaattaatgg aagaactcgg
    15481 tgttatagat ttaatgatta cagctcatca gcatcaaaca atagtaggtc aagatcatga
    15541 aacgtattat gttcaggctg gtcaagatgc caaagagctt gtacatcttt cgattaattt
    15601 taaaaagaga acaacaactt atgatgttga aagcattgat tctaaagtga ttgacttaaa
    15661 tgagtatgaa gaggatcaag aattattaga tttaacattc tatgatagaa aagcagtggc
    15721 ttattggtca caggaaatca taagtgataa aggtttgatg ttatcagtaa atgggttaca
    15781 agatttagtc tgtcaaacac atccattttc gcaattatta catgatgcaa ttcaccttgc
    15841 atttgataat gatataacat gtgtccacgt gcctatgaac ggagagaagg ggttgagtgg
    15901 acagattcga aatgaagatt tgtatcatgc atacccatat ccagataagc caatggatat
    15961 gacaattagt ggtcaaaata tcaaagatat attggagtat agttattcac atttagattt
    16021 tgttaacgag caattaagct taacaattat tgatgaaacg ttatgtacaa tgtggcaagg
    16081 attcaattat gagattgata tgaatcaaga acctgggcaa cgagtaatgt tagatcaaat
    16141 tgatttgact aagagttata gagttacaat gactgactat tgttatcgta actacaagaa
    16201 ttatttaaaa aatgctatta tacatgaatc atacgatgaa acaatgagta cattaattgc
    16261 agagaagtta agagatccga attatcgtat ttcatgtagt gat
//
LOCUS       DAKTGS010000056        15886 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.056, whole genome shotgun sequence.
ACCESSION   DAKTGS010000056 DAKTGS010000000
VERSION     DAKTGS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.056"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            459..2981
                     /gene="mprF"
                     /locus_tag="O0286_001458"
     CDS             459..2981
                     /gene="mprF"
                     /locus_tag="O0286_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCY0169841.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(3184..3693)
                     /gene="msrA"
                     /locus_tag="O0286_001459"
     CDS             complement(3184..3693)
                     /gene="msrA"
                     /locus_tag="O0286_001459"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0169842.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3830..4813
                     /locus_tag="O0286_001460"
     CDS             3830..4813
                     /locus_tag="O0286_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0169843.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(4901..5086)
                     /locus_tag="O0286_001461"
     CDS             complement(4901..5086)
                     /locus_tag="O0286_001461"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCY0169844.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            5234..6496
                     /locus_tag="O0286_001462"
     CDS             5234..6496
                     /locus_tag="O0286_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCY0169845.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(6633..7724)
                     /locus_tag="O0286_001463"
     CDS             complement(6633..7724)
                     /locus_tag="O0286_001463"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCY0169846.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            7889..8920
                     /locus_tag="O0286_001464"
     CDS             7889..8920
                     /locus_tag="O0286_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0169847.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            9412..10818
                     /locus_tag="O0286_001465"
     CDS             9412..10818
                     /locus_tag="O0286_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCY0169848.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            10815..11381
                     /locus_tag="O0286_001466"
     CDS             10815..11381
                     /locus_tag="O0286_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0169849.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            11387..12385
                     /gene="trpD"
                     /locus_tag="O0286_001467"
     CDS             11387..12385
                     /gene="trpD"
                     /locus_tag="O0286_001467"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCY0169850.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            12387..13169
                     /gene="trpC"
                     /locus_tag="O0286_001468"
     CDS             12387..13169
                     /gene="trpC"
                     /locus_tag="O0286_001468"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCY0169851.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            13169..13801
                     /locus_tag="O0286_001469"
     CDS             13169..13801
                     /locus_tag="O0286_001469"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCY0169852.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            13794..15008
                     /gene="trpB"
                     /locus_tag="O0286_001470"
     CDS             13794..15008
                     /gene="trpB"
                     /locus_tag="O0286_001470"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCY0169853.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            15001..15729
                     /locus_tag="O0286_001471"
     CDS             15001..15729
                     /locus_tag="O0286_001471"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCY0169854.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
BASE COUNT         5573 a         2290 c         2892 g         5131 t
ORIGIN      
        1 atatgtttgt tggtattttt tatggtgctt ataatgttgg gcagttacat ttatgattat
       61 cagcacatgt ttagcttata aatttttacg aaagtttagc cgtttataaa gcacatgcat
      121 aatgaaacga gtatttgcca cttgattagt acttcattat tatgtcgaaa ataaaaataa
      181 gtggtatttt taatatatta agaagcactc ataatcggct gttaattaat aatatttttc
      241 ataagtattg attcatcatt ttctttatgt taaatatgaa tcagtataga taaccatatt
      301 gttctgtttg agatagaaat attttctatt tacttcttaa tacagatatc aatatgacaa
      361 aagtgttata taaatcaaag gtaaatgata tgctattttt atggtttata tgatatattt
      421 tgatttataa cagaaataat tagaattgat gtgaaaaaat gaatcaggaa gttaaaaaca
      481 aaatattttc aatcttaaaa attacgtttg ctacagcttt atttattttt gtagcaatca
      541 cattgtatcg ggagttatct ggtattaact ttaaagatac gttggttgaa tttagtaaga
      601 ttaaccgtat gtccttagtg ttactattta ttggtggtgg ggcatcgctt gttattctat
      661 caatgtatga tgtgatttta tctagagctt taaaaatgga tatatcctta ggcaaagttt
      721 taagagtaag ttatatcatc aatgcattga atgcgattgt aggtttcggt ggctttattg
      781 gtgcaggcgt tagagcaatg gtttataaaa actatacgca tgataaaaag aaattagttc
      841 actttatatc cttaatactt atttcaatgt tgacaggttt aagcttatta tcattgctaa
      901 ttgtattcca tgttttcgat gcatctttaa tcttagataa gattacatgg gtaagatggg
      961 tattatatgt agtgtcattt ttcttaccat tattcattat ttattcaatg gttagaccac
     1021 ccgataaaaa caatcgtttt gtaggattgt actgcacttt agtgtcgtgt gttgaatggt
     1081 tagcagctgc agttgtatta tatttctgtg gtgtaattgt tgacgctcat gtatcattca
     1141 tgtcctttat tgcaatattt atcattgctg cattatcagg tttagtcagc tttattcctg
     1201 gtggtttcgg cgctttcgat ttagttgtat tactaggatt taaaacttta ggtgtccctg
     1261 aggaaaaagt attattaatg ctacttctat atcgttttgc gtactatttt gtaccggtaa
     1321 ttattgcatt aattttatca tcatttgaat ttggtacatc agctaagaag tacattgagg
     1381 gatctaaata ctttattcct gctaaagatg ttacgtcatt tttaatgtct tatcaaaagg
     1441 atattattgc taaaattcca tcattatcat tagcaatttt agtattcttt acaagtatga
     1501 tcttttttgt aaataactta acgattgttt acgatgcttt atatgatgga aatcacttaa
     1561 cgtattatat tctattggca attcatacta gtgcttgttt attactttta ctgaatgtag
     1621 ttggtattta taagcaaagt agacgtgcca ttatctttgc tatgatttca attttattaa
     1681 tcacagtggc gacattcttc acttacgctt catatatttt aataacatgg ttagctatta
     1741 tttttgttct gcttattgta gctttccgta gagcacgtag gttgaaacgc ccagtaagaa
     1801 tgagaaatat agttgcaatg cttttgttca gtttatttat tttatatgtt aaccatatat
     1861 ttattgctgg aacgttatat gcattagata tttatacgat tgaaatgcat acatctgtat
     1921 tgcgctatta cttctggctt acgattttaa tcatcgctat catcataggt atgattgcat
     1981 ggttgtttga ttatcaattt agcaaagtac gtatttcttc taaaattgaa gattgcgagg
     2041 agattattaa tcagtacggc ggtaattatt tgagtcactt gatatatagt ggtgacaagc
     2101 agtttttcac taatgaaaat aaaacagcat ttttaatgta tcgttataaa gcaagttcat
     2161 tagtggttct tggagatccg ttaggtgatg aaaatgcctt tgatgaattg ttagaagcat
     2221 tctataatta cgctgagtat ttaggctatg atgttatatt ctatcaagtt acagatcaac
     2281 acatgccttt atatcataat ttcggtaacc aatttttcaa attaggtgaa gaagcaatta
     2341 ttgatttaac gcaattttca acttcaggta aaaaacgccg tggatttaga gcgactttaa
     2401 ataaattcga tgaacttaat atttcgttcg aaattattga accaccgttt tcaactgaat
     2461 ttataaatga acttcaacat gtaagtgatt tatggctaga taatcgtcag gaaatgcatt
     2521 tctctgttgg tgaatttaat gaagaatact tatctaaagc gccaattggt gtaatgcgaa
     2581 atgaagaaaa tgaagtaatt gcattttgta gtttaatgcc aacatacttt aatgatgcca
     2641 tttcagtcga tttaattaga tggttgccag agttagattt accattaatg gatggtctat
     2701 acttgcatat gttactttgg agtaaagaac aaggttatac aaaatttaat atgggtatgg
     2761 caacgttatc gaacgttggt caattgcatt attcatattt aagagaacga cttgcaggcc
     2821 gtgtctttga acatttcaac ggtctatatc gtttccaagg attacgtcgt tataaatcta
     2881 aatataatcc gaattgggaa ccacgctttt tagtttatcg taaagataat tcgctttggg
     2941 aatcactttc taaagtaatg cgtgtaatac gtcacaaata attaaaatcc aagtgctaag
     3001 aggtatacag ttatgcctga tgcgcttgga ttttttggtt tctataaaga atgatttata
     3061 aaaaacggta cagataagaa tatttaagtt tcctatctgt accgtatttt aatattgaaa
     3121 tgagctaaag ttaagtcgcc taacttttat tatcagtcca atcagtttca tcaagtataa
     3181 atgttattgc ttatttttgt attcttggcg tattttttgt tcttctgcat agcgctctgg
     3241 atttttctta taaaaatctt ggtgatagtc ttcggctttg taaaattgtg acgctggtaa
     3301 tatttttgtt gcaattgcct tatcagcatt aatcgtattt ttaagctgct cgatataagt
     3361 ctcagcgagt tctttttgat gatcattagt gtagaaaata gctgtttgat attgaggacc
     3421 acggtcttga tattgaccac ctgtatctaa tgggtcaatg actgagaaaa atatttctaa
     3481 taacttattg tatgagaata atgcaacatc atattgaatt tcaacagttt ctaaatgacc
     3541 actcgtacct gattttactt gttcgtaagt aggattttca atatgtccgc ccatatatcc
     3601 agaagttact ttttctatgc cgtcaaaggt gtcaaatggt ttcgtcatac accaaaagca
     3661 acctccggca aaataagctg tattaatatt catttttgac atcctttcat tagaccttag
     3721 tacgatttat taagaaatca cttgcttttt gaattgtttt tatataacgt taatatgtga
     3781 ttattatagt acacaaaaat ctatttttga agatataagg taggtaaata tggataaaga
     3841 aactaatgac aacgaatata gacgtcaaag tgaacatcgc acttcggcgc ctaagcgaaa
     3901 aaagaagaag aaaattagga aattacctat cattcttctg attgttgtaa ttttacttat
     3961 cgcattagtt gtatatattg tacatagtta caatagcggt gtagaatatg ccaagaaaca
     4021 tgcgaaagat gttaaagtac atcaatttaa tggaccagta aaaaatgatg gtaaaatttc
     4081 tattcttgta ctcggtgcag ataaagcaca aggtggacaa tcaagaacag attctatcat
     4141 ggttgttcaa tatgacttta tcaataaaaa gatgaaaatg atgtctgtca tgcgtgatat
     4201 ttatgcagat attccaggat atggaaaaca caaaattaat tcagcatacg ctttaggtgg
     4261 tccagagcta cttagaaaaa cacttgataa aaatttagga attaatcctg aatattatgc
     4321 agtagttgat tttactggat ttgagaaaat gattgatgaa ttaatgcctg aaggtgtacc
     4381 aattaatgtc gaaaaagata tgtcgaaaaa tattggtgta tctttgaaaa agggtaacca
     4441 taggttgaat ggtaaagaat tacttggtta tgcaagattc cgtcacgacc ctgaaggtga
     4501 cttcggacgc gtgcgacgtc agcaacaagt gatgcaaaca ttgaaaaaag aaatggttaa
     4561 ttttagaaca gttgttaaat taccaaaagt tgcaggtatt ttaagaggct atgtgaatac
     4621 aaacattcct gattcaggga ttttccaaac aggtttgagt tttggtatcc gaggtgaaaa
     4681 agatgttaag tcattgactg tgccaatcaa gaactcatac gaagatgtca atacaaatac
     4741 tgatggtagt gcattacaga ttaataaaaa cacaaataaa caagctatta aagacttttt
     4801 agatgaagat taaaaataaa caaggcgatt tctatcatac tcagatagaa gtcgccttgt
     4861 tcgttttcaa agaattaatt ggcatgcatc ttatgaagaa ttattgatct gactttctta
     4921 cgccagccac accataatgg tttggtttca tttcttctat aacaacgtga attgcttgtc
     4981 tatttgcccc cgttgttttt tctacggcgt cagttacttc gctaactaaa ttttttaatt
     5041 gttcatccga acgaccttct aataatttta cattgacgat tggcatcatc caaacctcct
     5101 aatcattatt aatttgagta tatcatgtaa tacttaaaaa ttcatttgca aaataacaga
     5161 acatttgttc taaaatagtt gaaatagaac acgtgttcgt atataataag attcaagtaa
     5221 gggaggaata acagtgtata attatcattt attagaagat agggatgttc tatgtattga
     5281 ccaaaaaagt ttttttgcga gtgtttcttg tattgaaaag gggctagatc cattagaaac
     5341 aaagctagct gttgttgcag atactaagcg tcagggttct gtaatattgg ctgcgacacc
     5401 taaattaaaa gaattaggca tcaagacagg gtcgcgattg tttgaaatac cacatagaaa
     5461 tgatatttac attatcaatc caagtatgcg taaatatctt aatgtttcag ttgctatttc
     5521 taagattgca ttgcgttata ttccacctga agatttacac caatatagta ttgacgaatt
     5581 ttttatggat gttactgata gctatcatag atttagttct acagtacatg cattttgcga
     5641 aagacttaaa cgtgaaattt atgaagaaac aggcatttat tgtactgtgg gcattggttc
     5701 taatatgtta ttaagtaaaa ttgctatgga tgttgaagcg aagcatagtc aaaatggtat
     5761 agctgaatgg cgatatcaag atgtaccaac gaaattatgg ccaattcagc ccttgcgaga
     5821 tttttggggt attaatcgtc gaacagaagc caaattgaat aaaagaggaa tttttactat
     5881 aggagattta gcgaaatatc catataaatt tttaaaaaaa gagttcggta ttttaggtgt
     5941 tgatatgcat ctacatgcga atgggataga tcagagtaaa gtacgtgaaa agcacaagat
     6001 cagcaatcca tcgatatgca aaagtcaaat attaatgaga gattatcatt ttgatgaagc
     6061 aaaagtagta atgcaagagt taattgaaga tgttgctagc agagttcgag caagaaaaaa
     6121 agtggcaaga acgatacatt ttgcctttgg ctatagtgat gaaggcggtg tacataagca
     6181 atatactttg aaagatccaa caaacttaga aaaagatatt tataaagtag taatgcattt
     6241 cgcagataaa ttatgtaata aacaagcact atatcgtacg ctaagtatat ctttgagtca
     6301 atttattaat gaggatgagc gacagttaag tctgtttgaa gatgaatacc aacgcaaacg
     6361 tgacgaatgt ctagctaaaa cgatagacca attacatttg aaatacggca aaggtattgt
     6421 gtccaaagca gtatcgttta cagaagcagg tacaaaacac ggcagattag gtttaatggc
     6481 tggacataaa atgtaatgac tatacggttt aagtaatata taactgtgat tcgtataaaa
     6541 taagtctcta aagataaata tttcatatat cacaatagat tttcacaata atatctaaga
     6601 atacatggaa tttatcaaaa gagacttaat aattattgga tataacaatc aaaatcactc
     6661 aatgcttgca taccgcgttc tcggtcagta gggtttttga aactaatttt taaagcaccg
     6721 tatatatctt cgcgtacttc taagattctt aagttgctta tagatatgtt atgtaaactc
     6781 aggatataag tcactttact tatcatacct gattcatccg gaatgtctac atatagatca
     6841 tacgcagtat ttagtccacc tagttgttta gcgggtagtg cgtcgcgata cgatttagct
     6901 tgggcaaaaa atgataacaa tttttcagaa tcattgcttt caattagtct ttctaaatct
     6961 tgaaactgac tttttagctg tcgaatcatt tctaaaatat acgttttatt actcaaggtg
     7021 atatctttcc acatttgtgc attactacta gctatacgag tgatatcacg aaaaccacca
     7081 gctgcaagtt tattaactaa atgatgttct tgaccgttct tttgactaac atgaactaaa
     7141 ctagatgcaa cgatatgagg taaatgactt acgacgcttg ttacgtagtc gtgttcttca
     7201 gcagtagtta caataaattt agcaagagta ggtgataaca gttcttttaa cgtgtttgct
     7261 gcttgctcat ttcttggctc attgtagact aaaatataat aagcgttttc aaataagtgc
     7321 tttttagcat ttagtacacc agatttatga ctaccagcca ttggatgacc actgactaaa
     7381 tgaatattat gctttaataa attgcattcg tgttgctgta tcattgcttt agtactacca
     7441 gtatcagaaa caataacacc aggtttagtt ggcatatcta taagctcgct aagatattta
     7501 tttgtgatag caacaggtgt tgcataaata attacatcgg cttttttaat agcttcacta
     7561 taatttaaac atttttcatt aataatgccg attgatttag ctttatctaa ctgagaagta
     7621 tctgcatcgt atgcaataat attagtatta gggttatggt attttatatt gctagcaaga
     7681 cttccaccaa ttaatccaag cccaacaaat aaaactgttg tcatataaat caccttattt
     7741 cgaaattttc agaataataa cattgtaaat gagctgttga cacagtgcaa tagtaaataa
     7801 aaatcgataa tagcattaat agataaacgg agataaatca tctacaataa agagtatagt
     7861 aacacaatgg caacggaggg gtaaatcaat ggaaccaata ttagaaatga ttaaaacatt
     7921 aacaggtatt aatagtcctt caggagacac agaagaagca attcaatttg tcgaaaaata
     7981 tgcaaaagac ttgggttatc aaacaacact aacaaataaa ggtgcgttat taataacagt
     8041 gccaggcaaa aatgatgaag tacaacgctg tattactgct catgttgata ctttaggtgc
     8101 aatggttaaa gaaattaaag aagatggtcg cttagcaata gaattaattg gaggattcac
     8161 gtataacgcg attgagggtg aatattgcca aattaaaact gatgctggtc aaatatatac
     8221 aggaacaatt tgtctgcatg aaacaagtgt tcatgtatat agaaataatc atgaaatacc
     8281 tagagatcaa aagcatatgg aaataagaat tgatgaagta actacatcag aagaagatac
     8341 aaagagttta ggtatttcag taggtaattt tgttagcttt gatccacgta cagttatcac
     8401 gtcatcaggt tttattaaat ctcgtcattt agatgataaa gctagcgtag cgatgatact
     8461 acaattacta aagaaattaa aagaagagca aataatatta ccacatacaa cgcaatttta
     8521 tatttctaat aacgaagaaa taggttacgg tgcaaatgca tcaattgatt cgaaaatcaa
     8581 agaatatatt gcattagata tgggcgcgtt gggagacggt caagcatcgg atgaatatac
     8641 agtttctatt tgtgccaaag atgcttcagg tccatatcat aagcaattga aatcgcacct
     8701 agttaatctt tgcaaaataa ataacattcc atataaagta gacatatatc catattatgg
     8761 ttcagatgct tcagcagctt tacatgctgg tgcggatatc agacatggtt tatttggcgc
     8821 tggcattgaa tcatctcatg caatggaacg aacacatatt gattctatta aagcgacaga
     8881 gaaattacta tatgcatatt gcttatcacc aattgagtaa acaattagtg ttgacaaatg
     8941 tgaacgacct atgtaatata atgaactata aaaataatta gaattttcta aagaaatagt
     9001 agcagatatg aaacgtagca aatagaaagc taatgggtga tgggaattag cacgccatat
     9061 cttgtgaatt ggactttgga aaacaattga atgagttttg aaagtgaaca tgaattatgt
     9121 taactaaggt ggcaccacgg taacgcgtcc ttacaggtat atgcgttatg tggtgtcttt
     9181 ttatttagac aaaatgtagt agttaattaa aggtagcaac agaaagttag tggatgatgt
     9241 gaactaacac cgagattaat gaaattgggt tttgtctgca acagaaaaat tatatatagt
     9301 aaagagtgaa ctatgaatat ttcgaatatt cggttaattt aggtggtacc acgcgtcagc
     9361 gtcctttata ttgataagga tgctggcgct tttttgaaag gagcgtatag aatggatata
     9421 ttttataaaa aaataaaagc aaatgtaacg cccgaagttt tagcacaact tcattccaag
     9481 aagatcattt tggaaagtac aaatcaacaa caaactaaag gtcgctattc agttgttatt
     9541 tttgatattt atggcacttt aactttagat aatgatgtat tatcagtaag tactttaaaa
     9601 gaatcgtatc aaatcactga aagaccgtac cattatttaa cgactaaaat aaatgaagac
     9661 taccataata ttcaagatga gcaacttaag tcattaccat ttatatctgg atatgttggg
     9721 acgtgtagct ttgatttagt aagacatgaa tttcctaaat tgcaatcaat acaattagaa
     9781 gatcacaagc agcacgatgt aaggttatat atggttgaac aagtttatgt atttgaccat
     9841 tacaaagatg agttatatat catcgcgacg aatcaatttt caaattcaac aaaatcagat
     9901 cttgagaatc gagttattaa gtctatcgaa gacttaacta aaatccaacc attcatgcct
     9961 acacaagatt ttgattttaa aactaaagaa attcaatcaa acatttctga agaaagattt
    10021 atcgaaatga ttcagtattt caaagagaaa ataacagaag gggatatgtt ccaagttgtg
    10081 ccatcaagaa tttacaaata tgcgcatcat gctagtcagc atttaaatca actttcgttt
    10141 caactgtatc aaaatttaaa acgacaaaac ccaagtccat atatgtatta tcttaatatc
    10201 gatcaaccat atattgtcgg tagttctccc gaaagtttcg taagtgtcaa agatcaaatt
    10261 gtaacaacta atcctattgc aggtacgatt caacgtggtg agacgacaca aatagataat
    10321 gagaatatga aacaactact taatgatcca aaagaatgca gcgaacatcg tatgctagtt
    10381 gatttaggac gtaatgatat tcatagagta agtaaaatcg gtacctcaaa aattactgaa
    10441 ttaatggtta ttgaaaaata tgaacatgtt atgcatatcg taagtgaagt cacaggtaaa
    10501 ataaatcaaa atttatcgcc aatgacagtt attgcgaatt tattaccaac aggtaccgtt
    10561 tcaggtgcac caaaattacg tgcaattgaa agaatatatg aacaatatcc acataaacgg
    10621 ggcgtttata gtggtggtgt tggatacata aattgtaatc ataacttaga ttttgcatta
    10681 gcaattcgaa cgatgatgat agatgagcag tatatcaacg tagaagctgg ttgtggcgtt
    10741 gtatatgatt ctattcctga aaaagaactg aatgaaacga aattgaaagc taaaagctta
    10801 ttggaggtga gcccatgatc ttagttgtag ataattatga ttcctttaca tataacctag
    10861 tggatattgt tgctcaacat actgacgtca ttgttcaata ccctgatgat gataatgtgc
    10921 tgaatcaatc ggtggacgct gttattatat ctcctggtcc agggcatcca ttagacgatc
    10981 aacagttaat gaaaatcata tcaacctatc aacacaaacc cattttaggt atttgtttag
    11041 gggctcaggc actgacttgt tactacggtg gagaagtcat taaaggcgac aaggttatgc
    11101 acggcaaagt tgatacacta aaggttatat cgcatcatca acatctgtta tatcaagata
    11161 taccagaaca gttttcaatt atgagatatc attcattaat aagtaaccct gacaattttc
    11221 cagaagaatt gaaaattact ggacgtacca aagattgtat acagtcattc gagcataaag
    11281 aaagaccgca ttatggtatt cagtaccatc ctgaatcatt tgctacagac tatggtgtca
    11341 aaataattac aaatttcatt aatctagtga aggaaggatg aaaaccatga cattactaac
    11401 aagaataaaa actgaaacta tattacttga aagcgacatt aaagagctaa tcgatatact
    11461 tatttctcct agtattggaa ctgatattaa atatgaatta cttagttcct attcggagcg
    11521 agaaatccaa caacaagaat taacatatat tgtacgtagc ttaattaata caatgtatcc
    11581 acatcaacca tgttatgaag gggctatgtg tgtgtgcggc acaggtggtg acaagtcaaa
    11641 tagtttcaac atttcaacga ctgttgcttt tgttgtagca agtgctggcg taaaagttat
    11701 aaaacatggt aataaaagta ttacctcaaa ttcaggtagt acggatttgt taaatcaaat
    11761 gaacatacaa acaacaactg ttgatgatac acctaaccaa ttaaatgaaa aagaccttgt
    11821 attcattggt gcaactgaat catatccaat catgaagtat atgcaaccag ttagaaaaat
    11881 gattggaaag cctacaatat taaaccttgt gggtccatta attaatccat atcacttaac
    11941 gtatcaaatg gtaggcgtct ttgatcctac aaagttaaag ttagttgcta aaacgattaa
    12001 agatttaggt agaaaacgtg caatcgtttt acatggtgca aatggtatgg atgaagcaac
    12061 actatctggt gataatttga tatatgaatt gactgaagat ggagaaatca aaaattacac
    12121 attaaatgcg actgattatg gtttgaaaca tgcgccgaat agtgatttta aaggcggttc
    12181 acctgaagaa aatttagcaa tctcccttaa tatcttgaat ggtaaagatc agtcaagtcg
    12241 acgtgatgtt gtcttactaa atgcgggttt aagcctttat gttgcagaga aagtggatac
    12301 catcgcagaa ggcatagaac ttgcaactac attgattgat aatggtgaag cattggaaaa
    12361 ataccatcaa atgagaggtg aataatatga cgattttatc agaaattgtt aaatataaac
    12421 agtcactttt acaaaatggc tattatcaag acaaacttaa taccttgaaa agtgtgaaga
    12481 ttcagaataa aaaatctttt ataaacgcaa ttgagaaaga accaaagcta gcaattattg
    12541 cagaaattaa atcgaagagt cctacagtta atgacttacc tgaacgagat ttatcgcaac
    12601 aaatctcaga ttatgaccaa tatggtgcaa atgccgtgtc cattttaact gatgaaaagt
    12661 actttggtgg tagttttgaa agattacaag cattgacgac aaaaacaaca ttacccgtat
    12721 tatgcaaaga ctttattata gacccgcttc aaattgatgt tgctaaacaa gctggtgcat
    12781 ctatgatttt attgatcgtt aacatcttat ctgataaaca attgaaagat ttatataact
    12841 acgctatatc gcaaaatcta gaagtgttag ttgaagtaca tgatcgccat gaattagaac
    12901 gtgcctataa ggttaatgct aaattgattg gtgtaaataa cagggactta aaacgatttg
    12961 ttacaaatgt ggaacataca aatactattt tagaaaataa aaaaacaaat cattattata
    13021 tttctgaaag tggtattcac gatgcatctg atgtaagaaa aatcttgcat agtggtatcg
    13081 atggcttact aataggtgag gcgcttatgc gttgtgacaa tctatctgaa tttttaccac
    13141 aactgaaaat gcaaaaggtg aagtcatgat gaaattgaaa ttttgtggct ttacatcaat
    13201 aaaggatgtt acagcggcca gtcaattacc tattgatgcg ataggtttca tccattatga
    13261 aaaaagtaaa aggcatcaaa caattaccca aataaaaaag ttagcgtctg ctgttccaaa
    13321 tcatatcgat aaagtatgtg tcatggtaaa tcctgattta acaacaattg aacacgtatt
    13381 aagcaatacg tcaattaaca caatacagtt acacggcaca gaatctattg attttataca
    13441 ggaaattaaa aagaaatatt caagcattaa aatcactaaa gctttagctg cagatgaaaa
    13501 cataatccaa aacataaata aatataaagg gttcgtagat ttatttatta tcgacacacc
    13561 ctcagtgtcg tatggtggta ccggtcaaac atatgactgg actattttga agcacataaa
    13621 agacatacct tatttgatag caggaggcat taactctgaa aatattcaaa cagttaatca
    13681 acttaaatta tcacatcaag gttatgatct tgcatcaggt atagaagtaa atgggcgaaa
    13741 agatatagaa aaaatgacag caattgtaaa tattgtgaaa ggagatagag acaatgaata
    13801 aacaaataca aacagaagca gatgaattag gtttctttgg tgaatatgga gggcaatatg
    13861 ttccagaaac attaatgcca gcaattattg agttgaaaaa agcttataaa gaggcaaaag
    13921 cagacccaga gtttcaaaga gaactggaat actatttatc agagtatgta ggacgcgcga
    13981 caccacttac atatgctgca tcatatactg aaagcctagg tggcgctaaa atatatttga
    14041 aacgagagga tctaaatcat acaggcgccc ataaaattaa taatgcgtta ggtcaagcgt
    14101 tgcttgctaa aagaatgggc aagaagaagc ttgttgctga aactggtgcg ggtcaacatg
    14161 gtgtagctag tgctacggtt gctgcattat ttgatatgga acttgttgtc tttatgggaa
    14221 gtgaagatat taaaagacaa caacttaatg tatttagaat ggaattactt ggtgcaaagg
    14281 ttgtggcagt tgaagatggt caagggactt tatcggatgc agttaataaa gcattgcaat
    14341 attgggtaag tcatgtagat gatacacatt atttattagg ttctgcatta ggtccagacc
    14401 cgttcccaac gattgttaga gattttcaga gtgtgattgg taaagaaata aaatcacaga
    14461 tattgaagaa agaaggtcga cttccggatg caattgtagc atgtatcggt ggtggctcaa
    14521 atgcaatcgg tacattttat ccatttatta aagatgatgt tgcattatac ggtgttgaag
    14581 ccgcaggtca aggcgatgat actgataaac atgcacttgc aattggcaaa ggatcacctg
    14641 gcgtattaca tggtactaaa atgtatttaa ttcaagatga agatgggcaa gtgcaactag
    14701 cacattctat ttcagcagga cttgattatc ctggtattgg accagaacat tcttattacc
    14761 acgacattgg tagagtaact tttgaaaatg ctagtgatac acaagcaatg aatgctttaa
    14821 tcaactttac aaaacatgaa ggtattatac ctgcaattga aagtgcacat gcactgagtt
    14881 atgttgaaag actagcgcct acgatgtcga aagaagatat tattgtagta actatttctg
    14941 gacgtggcga taaagatatg gaaacaatta gacaatatat ggtagagcga ggtcttgcaa
    15001 atgactaaat tatttatacc ttatattatg ggcaataaag atttgattga aaatgcaaca
    15061 ttgttgagtg aaaatggtgc agatataatt gaaattggag tacctttctc tgatccggtt
    15121 gctgatggtc cagttatcat ggaagcaggt caacaagcga ttaaacaagg catcacgata
    15181 gattatattt tcaatcaatt agaaaaacat ggtgatcaaa ttaagtgtaa ctatgtatta
    15241 atgacgtatt ataatattat ttgtcattat ggagaacaag cgttttttga aaaatgtcga
    15301 gatactggtg tctacggctt aattattcct gatttaccat atgaattatc gcagcgttta
    15361 aaacaacaat ttagtcacta tggcgtcaaa atcatatcgt tagttgcgat gactactgat
    15421 gacaaacgta taaaagatat cgtatcccat gcggaaggct ttatttatac tgtgacgatg
    15481 aatgcgacaa cagggcaaaa cggtgcgttt catccagaat taaaacgaaa aattgagtca
    15541 attaaagcga tagccaatgt gccagttgtc gcaggatttg gtataagaac accacaacat
    15601 gttgcagata taaaagaggt tgcagatggc attgtcattg gtagcgaaat cgttaagcga
    15661 tttaaatcta acacgcgtga ggaaatcatt aaatatttac aatctatcca acaaacattg
    15721 aataattaag tttacttgat ttaaaaaaat taggcgaata ctgtttgaaa aagtgaaaaa
    15781 cggtgaatta taaaattgaa tagaaatgat acaaaagtaa tatgagcaaa cccaaacgtt
    15841 catattactt tttttgaaat tgtattcaaa aatctaaata ttacta
//
LOCUS       DAKTGS010000057        15851 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.057, whole genome shotgun sequence.
ACCESSION   DAKTGS010000057 DAKTGS010000000
VERSION     DAKTGS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.057"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..375
                     /locus_tag="O0286_001472"
     CDS             <1..375
                     /locus_tag="O0286_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169855.1"
                     /translation="SSRYLTKYLNYKFVSEICLVIFLIIYTYQSFIAVTISMIFLGIS
                     SGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGYLYTIGTIQYLILFISL
                     LIFIYLIIIFYFTRREQHENKN"
     gene            359..874
                     /locus_tag="O0286_001473"
     CDS             359..874
                     /locus_tag="O0286_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCY0169856.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            1185..1619
                     /locus_tag="O0286_001474"
     CDS             1185..1619
                     /locus_tag="O0286_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0169857.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(1876..2061)
                     /locus_tag="O0286_001475"
     CDS             complement(1876..2061)
                     /locus_tag="O0286_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCY0169858.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            2356..3297
                     /locus_tag="O0286_001476"
     CDS             2356..3297
                     /locus_tag="O0286_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCY0169859.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            3309..4553
                     /gene="fabF"
                     /locus_tag="O0286_001477"
     CDS             3309..4553
                     /gene="fabF"
                     /locus_tag="O0286_001477"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCY0169860.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(4608..4979)
                     /locus_tag="O0286_001478"
     CDS             complement(4608..4979)
                     /locus_tag="O0286_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCY0169861.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            5222..6148
                     /locus_tag="O0286_001479"
     CDS             5222..6148
                     /locus_tag="O0286_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169862.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            6148..7218
                     /locus_tag="O0286_001480"
     CDS             6148..7218
                     /locus_tag="O0286_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169863.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            7235..8317
                     /locus_tag="O0286_001481"
     CDS             7235..8317
                     /locus_tag="O0286_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169864.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            8307..9248
                     /locus_tag="O0286_001482"
     CDS             8307..9248
                     /locus_tag="O0286_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0169865.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            9267..10922
                     /locus_tag="O0286_001483"
     CDS             9267..10922
                     /locus_tag="O0286_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0169866.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            11134..12849
                     /locus_tag="O0286_001484"
     CDS             11134..12849
                     /locus_tag="O0286_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0169867.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            12900..13886
                     /locus_tag="O0286_001485"
     CDS             12900..13886
                     /locus_tag="O0286_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169868.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            13889..14869
                     /locus_tag="O0286_001486"
     CDS             13889..14869
                     /locus_tag="O0286_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCY0169869.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            14862..15824
                     /locus_tag="O0286_001487"
     CDS             14862..15824
                     /locus_tag="O0286_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169870.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
BASE COUNT         5829 a         2173 c         2912 g         4937 t
ORIGIN      
        1 tcttcacgtt atttaactaa atacttgaat tataagtttg tgtcagaaat ttgtttagta
       61 atatttttaa taatatatac atatcaaagt ttcatagcag ttactatttc tatgatattt
      121 ttaggtattt cttcagggtt aactcgtcca caaactataa ataaactttc tagcagtagt
      181 aacttaagag tgatgcttaa ttatgcagaa acgttatatt ttatttttaa tatcgcattt
      241 ttacttatgg gtggttactt atatacaata ggaactattc aatacttaat attatttatt
      301 tcgttattaa tttttatata tttaataata atattttatt ttacaaggag agagcaacat
      361 gaaaataaaa actgaattta aagggaacaa tataccatat gaatacgcag caggtgcaga
      421 tgtgagtgat tctattaacg ggaatccaat taagtcattt ccatttgaag taattgaatt
      481 accggaaggg actaaatatc ttgcttggtc tttaattgac tatgatgcaa ttcctgtatg
      541 tggctttgct tggattcatt ggagtgtagc taatgtaagt gttagtggca attcaatttc
      601 tataaaagca gatttatcaa gaacaaaggg cgactatgta caaggtaaaa atagctttac
      661 tagtgggttg ttggctgaag atttttcaga aatagaaaat cactatgtag gacctacacc
      721 acctgatcaa gatcatcaat atgaattaac agtttatgcg ttagatcatt ctttaaattt
      781 gaagaatggg ttctacttga atgaattttt aaaagaagta aatcaacata aaattgatca
      841 aacaagtatt aaccttatag gaagaaaaat ttaatactaa atatctcatc aatataaaat
      901 tgttcaatta aaagtacaaa gaaacaaagg ttttaattta tatattaggt acggcgttcg
      961 ctataatgca aagaagtaat taaatttaag aaatgtaaac ttagttattg taatgtgaat
     1021 ttatttgaaa aaatagaaag tattaacaat tatagctttt acattaatta aaatttattt
     1081 ttaaaaacaa gtaaacaatt tacatactta taatttttga aacttttcaa tttgtgttat
     1141 attgattttg taagatactt taactcacaa aggagagaga ttatatgaaa ttaaaatcat
     1201 ttataactgt aactttggca ctgggcatga tcgcaacgac tggcgctact gtggcaggta
     1261 atgaggtatc tgcagcagaa aaggacaaac taccggcaac tcaaaaagct aaagaaatgc
     1321 aaaatgttcc atatacaatt gcagtagatg gcattatggc tttcaatcaa tcttacttaa
     1381 atttaccaaa agatagccaa ttatcatatt tagatttagg aaataaagtt aaagctttgt
     1441 tatatgatga acgcggtgta acacctgaga agattcgaaa tgcaaaatct gccgtttaca
     1501 cgattacttg gaaagatggt agtaaaaaag aagtggatct taagaaagat agctacacag
     1561 caaacttgtt tgattcaaat tcaattaaac aaattgatat taatgtaaaa actaaataaa
     1621 cacttaaaat tatcaaattc aacatgaagc atttaatttt acagtgatga ttataaaata
     1681 attgccttga tacaaagatt actcgtaaat gacatctttg tattaaggct ttttctaaat
     1741 ttaaaagtga tgggttagag gtcattgagc tttaaaatat tcaaaataca aaacattaat
     1801 ggccaaaaat aaaagccgcc tttatctggg cagcttcaat aataagaaag acatatttca
     1861 ttttatacta aatagttatt gtgatgaatc tttcggcggt ttaattactg cagcaaaaat
     1921 tgctgtgaaa atcgtgaaca atactgccat gataattgga ttcactacat ttaagctgtc
     1981 tccacctact aggctattaa gtacaaagtt aaccatttgc attaataata atgcccaaaa
     2041 gaatgttacg aggtgtttca tgtcattcta cctccacttt aattatatat attttatttt
     2101 aagtgaaagt tagaaatttg tatagtaaca tctcatatat tttgaccata ttatacagtt
     2161 taaataaatg attttatctg aatggctatt ctaaattaag cgcattaaaa ccaatttcat
     2221 actgaaattt gacgataata aagcattaaa attttattaa ctagtcaata ttcctacctc
     2281 tgacttgagt ttaaaaagta atctatgtta aattaatacc tggtattaaa aattttatta
     2341 agaaggtgtt caactatgaa cgtgggtatt aaaggttttg gtgcatatgc gccagaaaag
     2401 attattgaca atgcctattt tgagcaattt ttagatacat ctgatgaatg gatttctaag
     2461 atgactggaa ttaaagaaag acattgggca gatgatgatc aagatacttc agatttagca
     2521 tatgaagcaa gtttaaaagc aatcgctgac gctggtattc agcccgaaga tatagatatg
     2581 ataattgttg ccacagcaac tggagatatg ccatttccaa ctgtcgcaaa tatgttgcaa
     2641 gaacgtttag ggacgggcaa agttgcctct atggatcaac ttgcagcatg ttctggattt
     2701 atgtattcaa tgattacagc taaacaatat gttcaatctg gagattatca taacatttta
     2761 gttgtcggtg cagataaatt atctaaaata acagatttaa ctgaccgttc tactgcagtt
     2821 ctatttggag atggtgcagg tgcggttatc atcggtgaag tttcagatgg cagaggtatt
     2881 ataagttatg aaatgggttc tgatggcaca ggtggtaaac atttatattt agataaagat
     2941 actggtaaac tgaaaatgaa tggtcgagaa gtatttaaat ttgctgttag aattatgggt
     3001 gatgcatcaa cacgtgtagt tgaaaaagcg aatttaacat cagatgatat agatttattt
     3061 attcctcatc aagctaatat tagaattatg gaatcagcta gagaacgctt aggtatttca
     3121 aaagacaaaa tgagtgtttc tgtaaataaa tatggaaata cttcagctgc gtcaatacct
     3181 ttaagtatcg atcaagaatt aaaaaatggt aaaatcaaag atgatgatac aattgttctt
     3241 gtcggattcg gtggcggcct aacttggggc gcaatgacaa taaaatgggg aaaataggag
     3301 gataacgaat gagtcaaaat aaaagagtag ttattacagg tatgggagcc ctttctccaa
     3361 tcggtaatga tgtcaaaaca acatgggaga atgctctaaa aggcgtaaat ggtatcgata
     3421 aaattacacg tatcgatact gaaccttata gcgttcactt agcaggagaa cttaaaaact
     3481 ttaatattga agatcatatc gacaaaaaag aagcgcgtcg tatggataga tttactcaat
     3541 atgcaattgt agcagctaga gaggctgtta aagatgcgca attagatatc aatgaaaata
     3601 ctgcagatcg aatcggtgta tggattggtt ctggtatcgg tggtatggaa acatttgaaa
     3661 ttgcacataa acaattaatg gataaaggcc caagacgtgt gagtccattt ttcgtaccaa
     3721 tgttaattcc tgatatggca actgggcaag tatcaattga cttaggtgca aaaggaccaa
     3781 atggtgcaac agttacagca tgtgcaacag gtacaaattc aatcggagaa gcatttaaaa
     3841 ttgtgcaacg cggtgatgca gatgcaatga ttactggtgg tacagaagca ccaattactc
     3901 atatggcaat tgctggtttc agtgcaagtc gagcgctttc tacaaatgat gacattgaaa
     3961 cagcatgtcg tccattccaa gaaggtagag atggttttgt tatgggtgaa ggtgctggta
     4021 ttttagtaat tgaatcttta gaatcagcac aagctcgagg tgccaatatt tatgctgaga
     4081 tagttggcta tggtactaca ggtgatgctt atcatattac agcgccagct ccagaaggtg
     4141 aaggtggttc tagagcaatg caagcagcta tggatgatgc tggtattgaa cctaaagatg
     4201 tacaatactt aaatgcccat ggtacaagta ctcctgttgg tgacttaaat gaagttaaag
     4261 ctattaaaaa tacatttggt gaagcagcta aacacttaaa agttaactca acaaaatcaa
     4321 tgactggtca cttacttggt gcaacaggtg gaattgaagc aatcttctca gcgctttcaa
     4381 ttaaagactc taaagtcgca ccgacaattc atgcggtaac accagatcca gaatgtgatt
     4441 tggatattgt tccaaatgaa gcgcaagacc ttgatattac ttatgcaatg agtaatagct
     4501 taggattcgg tggacataac gcagtattag tattcaagaa atttgaagca taactataaa
     4561 aatcttcagt aacgttgttt tagttactga agattttttc agtttcttta tactaagatg
     4621 agcgacagca caatcgtcat aataaaatat aaaatattta ttaataataa ggggattatc
     4681 catgtagaaa caaagtaatg ctcttttttt acctcttgtg ggttgaaaaa tggatcatca
     4741 gagatagact tcttcttttt cgaagatgac atttgatact ttaatcttct aaaaccataa
     4801 cttgtcgcat caaaaatgcc ttcttgtaca agtaaaatca aaaatatgct aataaaaata
     4861 attaatgaaa cataaaacaa tatatttaaa tatgtaatga tagtatggct attaaaaagc
     4921 catataataa acgttaatat tggcgttatt agtgccattc caagccattt tttcaacatt
     4981 tgatcactcc cacttataga aaactcttac gcatagttta cattaaaatc agacattgag
     5041 gaatgatttt ttaatttctt cagctttatt gaaattctaa aatcaatcat tcttcattag
     5101 tttaaagcaa aaaaatattg atatatagta aatattgtat atataatatt agttaagatt
     5161 tcagaaaatt ttgaagggaa tggaaattta gaaatcggaa tttgttagag gaggggatta
     5221 gatggggaaa tatattttca aacgatttat ttatatgctt atttctttat ttattattat
     5281 tacaattaca tttttcttaa tgaaattaat gccaggttcg ccatttaacg atgctaaatt
     5341 aaatgctgaa caaaaagaaa ttttaaatga aaaatatgga ttaaatgatc ctgtagctac
     5401 gcagtattta cattatttaa aaaatgttgt tacaggcgat tttggtaatt cattccagta
     5461 tcataatcaa cctgtgtggg atttgattaa accgagacta ctaccttctt ttgaaatggg
     5521 tcttacagca atgttcatcg gtgtgatact gggacttatt ttaggtgttg cagcagctac
     5581 taaacaaaat tcttgggttg actatacaac tacagttatt tcagttattg cagtatctgt
     5641 accatctttt gtacttgctg tacttttaca atatgtattt gcagttaaat taagatggtt
     5701 cccagtagct ggatgggaag gtttttcgac cgcggtatta ccgtcacttg cattatctgc
     5761 agctgtttta gcaactgtcg ccagatacat aagagcagag atgatagagg tattaagttc
     5821 agactatatt ttattagcga gagctaaagg taattcgaca atgcgtgtac tttttggaca
     5881 tgcacttaga aatgctttaa ttccaattat tacaattatc gttcccatgt tagcaagtat
     5941 tttaacaggc actttaacaa ttgaaaatat ttttggagtt cctggattag gggatcaatt
     6001 cgtacgttca attacaacaa atgatttctc agtaatcatg gcaatcacac tattatttag
     6061 cacactgttt atcgtttcta tttttattgt agatattttg tacggtgtga tagatccacg
     6121 aattcgtgtt caaggaggta aaaaataatg gctgaaaata aaaacaattt gtcgattaac
     6181 gacgatcatt ctaatgcagc tatgacgcat acctctgacg ctatcgcatc atctgatttt
     6241 attattagag aattagattt gaatcaggaa cctgaaatgc aacgagaaag caaaaacttt
     6301 tggcaagatg cttgggctca gttaaaacga aataagttag ctgttgtcgg tatgataggt
     6361 ttaattatca ttgtaatatt tgcttttatc ggtccagtta taaataaaca tgattatgct
     6421 gaacaaaatg tagaacatag aaatcttccg gcaaaaatac ctgtattaga caaagttcca
     6481 tttttacctt ttgatggtaa agatgcagat ggcaaggatg cttataaagc agcaaatgct
     6541 aaagaaaatt attggtttgg tactgatcag ttgggtcgag atttatggac aagaacatgg
     6601 aaaggtgctc aaatttcatt gtttatcggt gttgttgcag cgatgttaga tatttttatt
     6661 ggtgttgtat atggtgcgat ttctggattc ttcggtggac gtgtcgatac gattatgcaa
     6721 cgtatacttg aagtcatagc atctattccg aatttaattg tcgtaatttt atttgtatta
     6781 atttttgaac catccatttg gacaattata ttggctatgt ctatcacagg ctggttaggc
     6841 atgagcagag ttgtacgtgg agaattttta aaattaaaaa atcaagagtt tgtcatggct
     6901 tcgaaaacat tgggggcttc aaaattcaaa ttgatattta agcatatttt acctaataca
     6961 ttaggtgcta tcgtggttac atcaatgttt acagtaccta gtgctatttt cttcgaagca
     7021 tttttaagtt tcattggtat aggtgtaccc gcacctcaaa catcgttagg gtcattagta
     7081 aatgatgggc gcgcaatgtt attaatttat ccacatgaat tatttatacc agcaatgatt
     7141 ttaagtttat taattctatt cttttactta tttagtgatg gattacgtga tgcatttgat
     7201 ccgaaaatgc gtaaataaaa agggggcata gcatatgact gaaagaatat tagaagtaaa
     7261 tgatttgcat gtttcctttg atattacagc aggggaagtg caggcagtga gaggcgtaga
     7321 tttttatttg aacaaagggg aaacattggc aattgttggt gaatcaggtt caggtaaatc
     7381 tgtaacaaca aaagcaatta caaaattatt ccaaggggac acaggaagaa ttaaaaaggg
     7441 agaaatttta tttttagggg aagatttagc aaaaaaacct gaaaatgagt tgattaaatt
     7501 acgtggcaaa gatatttcaa tgatctttca agatccaatg acatctttaa acccaacgat
     7561 gcaaattggt aaacaagtca tggaaccatt aattaagcac aaaaattata gtaaagcaca
     7621 agctaaaaag cgcgcattgg aaatactaaa tcttgtaggt ttaccaaatg cagaaaaaag
     7681 atttaaagca tatcctcatc aattttcagg tggacaaagg caaagaattg ttattgcaac
     7741 cgcattagct tgtgaaccta aagtgctcat tgctgatgaa ccaacgactg cattagacgt
     7801 aacgatgcag gcacaaattt tagatttaat gaaagaacta caacaaaaaa tcgatacagc
     7861 aattattttt ataacgcatg atttaggggt tgttgcgaat attgctgata gagtggcagt
     7921 tatgtatggt ggtcaaatgg ttgaaacagg agatgttaac gaaatatttt atgatccaaa
     7981 gcatccatat acatggggat tattatcgtc aatgcctgat ttatcaacaa caaatgacac
     8041 accattacta gcgattcctg gagcgccacc tgatttatta cacccaccta aaggtgatgc
     8101 atttgcgaga cgtagtcaat atgcattaga tattgatttt aaagtagaac caccgtggtt
     8161 taaagtttca ccgacacatt ttgtgaaatc ttggttatta gacgcacgtg caccaaaagt
     8221 tgaactaccc gagctggtaa aacaacgtat gaaaccgatg cctaataatt atgaaaaacc
     8281 actcaaggta gaaagggtgt cgttcaatga aaaatgatga agtgctatta tctattaaaa
     8341 atttaaagca atattttaac gcaggaaaga aaaacgaagt gagagcgatt gaaaatattt
     8401 cgtttgatat atacaaaggg gaaacattag gtttagtagg agaatcgggg tgtggtaaat
     8461 ctacaactgg taaatcaatt attaaactta atgatattac aagtggagaa attttgtatg
     8521 agggtattga tatacaaaag attcgtaaac gtaaagattt gcttaaattt aataaaaaga
     8581 tacagatgat ttttcaagac ccatatgcgt ctttaaatcc taggttaaaa gtaatggata
     8641 tagtagctga aggtattgat atccatcatt tagcaactga taagcgtgac cgaaaaaaac
     8701 gtgtctatga tttacttgaa actgttggat taagtaaaga acatgccaat cgctatcctc
     8761 atgaattttc aggtggacaa cgccaacgta ttggaattgc ccgtgcatta gccgttgaac
     8821 cagaattcat tatcgcggac gaaccaatat cggcattgga tgtttcaatc caagctcaag
     8881 tagttaattt attattaaaa ttacaacgtg aaagagggat tacgttccta tttatagctc
     8941 atgatctatc aatggtgaag tatatttcag atcgtattgc agtcatgcat tttgggaaaa
     9001 tagttgaaat tggaccggca gaagaaattt atcaaaatcc attacacgat tatactaagt
     9061 ctttattatc agccattcca caacctgatc ctgaatcaga acgcagtcgc aaacgattta
     9121 gttatattga tgatgaagca aataatcatt taagacaatt acatgaaatt agaccgaatc
     9181 actttgtctt tagtactgaa gaagaagcgg cacaactacg agaaaataaa ttggtgacac
     9241 aaaattaagg ggaaggggga aatgcaatga cgagaaaatt tagaacactt attttaattt
     9301 tgattgctac aattgcatta agtggttgtg ctaatgacga tggtatttat tcagataaag
     9361 gtcaagtatt cagaaaaatt ttgtcatcag acttaacatc ccttgataca tcattaataa
     9421 cggatgaaat atcttctgaa gtgactgcgc aaacattcga aggtttatac acattaggaa
     9481 aaggtgacaa accggtgtta ggtgttgcga aagcttttcc tgaaaagagt aaagatggta
     9541 aaactttaaa ggttaaatta agaagcgatg ctaaatggag caatggtgac aaagtgactg
     9601 cacaagactt tgtttatgct tggagaaaaa cagttgaccc taaaacaggt tctgaatttg
     9661 catacattat gggggacatt aaaaatgcga gtgatattag tactggtaag aaacctgtag
     9721 agcaattagg tatcaaagca ttaaatgatg aaacattaca aattgaatta gaaaagccgg
     9781 ttccatatat taatcaatta ttagcactta atacctttgc acctcaaaat gaaaaagttg
     9841 ccaaaaaata tggtaaaaat tacggtacgg cagctgatag agcggtatac aatggtccat
     9901 ttaaagttga tgattggaaa caagaagata aaaccttact atctaaaaat cagtattatt
     9961 gggataaaaa gaatgtaaaa ttagataaag tgaattataa agttattaaa gacttacaag
    10021 ccggtgcatc attgtatgat actgaatcag tagatgacgc agttattact gcagatcaag
    10081 taaataaata taaagacaac aaaggattaa actttgtgtt aacgactggg acattttttg
    10141 taaaaatgaa tgaaaaacaa tatcctgatt ttaaaaacaa aaatttaaga ttggctatcg
    10201 cacaagcaat agataaaaaa ggatacgttg attcagtgaa aaacaatggc tcaattcctt
    10261 ccgatacact aacagccaaa ggaattgcga aagcgcctaa tggcaaagat tatgcgagta
    10321 ccatgaattc gcctttaaaa tataatccta aagaagcaag agcgcactgg gacaaagcta
    10381 aaaaagagtt aggtaaaaat gaagtgacat tttcaatgaa cacagaagat acaccagatg
    10441 caaaaatatc tgctgaatat atcaaatcgc aagttgagaa aaatttacca ggagttactt
    10501 tgaaaattaa gcaattaccg tttaaacaaa gagtatcact agaactgagt aacaattttg
    10561 aagcatcact tagtggttgg tctgcagatt accctgatcc tatggcttat ttagaaacaa
    10621 tgaccacagg tagcgcacaa aataatacag actggggtaa taaagaatat gatcaattac
    10681 ttaaagtagc aagaaccaaa ttggcacttc aaccgaacga acgatatgaa aacttgaaaa
    10741 aagcagaaga aatgttccta ggagatgcac cggtagcacc aatttatcaa aaaggtgttg
    10801 cacatttaac aaatcctcaa gtaaaaggat taatttacca taaatttggt ccaaataact
    10861 cacttaaaca tgtatatatt gataaatcga tagataaaga aacaggtaag aagaaaaaat
    10921 aatatgcttt gtaaattagg ctggagacat atctccagtc tttttgtgtt ggataaaaac
    10981 tttgggaata aaaatttaaa ataagtcgtt ttttaaatta ctgaaattga ttaaatgcat
    11041 aaataactga atattctaaa aataaacttg taataatttt ttctatgagt aaactaaaaa
    11101 gaaaaaatta gattgaaagt aggaggcata tgtatgggga agctaattaa atatatttca
    11161 atacttctta ttgtcgtttt agtgttgagt gcttgcggaa aaagcagtaa taaagatgaa
    11221 ggagtaaaag atgctactaa aacggaaacc tcaaaacata aaggtggtac cttaaatgta
    11281 gcattaacag caccgccaag tggtgtttat tcttcgttat taaatagtac acatgcagat
    11341 tctgtagttg agggatattt taacgaaagc ttattagcaa ctgataaaaa aatacgtcct
    11401 aaggcatata ttgcttcatg gaaggacatc gagccggcta agaaaataga atttaaaatt
    11461 aaaaaaggta ttaaatggca tgatggtaat gaattgaaaa ttgatgattg gatttattca
    11521 attgaagtct tagctaacaa ggactacgaa ggtgcttatt atccaagtgt agaaaatatc
    11581 caaggtgcga aagattatca tgaaggaaaa actgatcata ttagcggatt gaagaaaata
    11641 gatgactaca ctatgcaggt tacatttgat aaaaaacaag aaaattactt aacaggattt
    11701 attactggac ctttattaag taaaaaatat ttatcagatg taccaattaa agatttagcg
    11761 aaatcagata aaatccgaaa atatcctatt ggtattggac cgtataaagt taagaaaatc
    11821 gttccaggtg aggctgttca actcgttaaa tttgatgatt attggcaagg taagcctgca
    11881 ctagacaaaa tcaatttaaa agttattgat caagcgcaaa ttattaaggc aatggaaaaa
    11941 ggcgatattg atgttgcgaa tgatgctacc ggtgcaatgg caaaagatgc taagtcatct
    12001 aatgctggtc tcaaggtatt atctgcgcca agcttagact acggtttaat aggattcgta
    12061 tctcatgatt acgataaaaa agctaataaa actggtaaag tgagaccaaa atatgaagac
    12121 aaagaattac gtaaagcaat gctttatgca attgatagag aaaaatggat caaagcgttt
    12181 ttcaatggtt acgctagtga aatcaatagt tttgtaccat ctatgcattg gatagcagcc
    12241 aatcctaagg acctaaatga ttacaaatat gatcctgaaa aagctaaaaa aatcttagat
    12301 aagttaggtt ataaagatag agatggtgac ggatttagag aagatcctaa aggtaataaa
    12361 tttgagatta actttaaaca ttattcaggt tctaatccta cttttgaacc aagaactgct
    12421 gcgataaaag atttctggga aaaagttggc ttgaaaacaa atgtgaagtt agtagaattc
    12481 ggtaaatata atgaagactt agcaaatgca tctaaagata tggaagtgta cttcagatca
    12541 tgggcaggag gtacagatcc agatccatca gatttatacc acactgatag acctcaaaat
    12601 gaaatgagaa cagttttacc aaaatcagat caatatttag atgatgcatt agacttcgaa
    12661 aaagtaggca ttgatgaaaa gaaacgtaaa gatatttatg ttaaatggca aaaatatatg
    12721 aatgatgagt tacctggatt accaatgttc caaggtaaat cgataactat tgttaacgat
    12781 aaagtacgaa acttagacat tgaaattgga actgatcaaa gtttatataa tttaactaaa
    12841 gaagcttagt aatgtaacct cgacgatttt gtcgaggttt acttatatgg ggggatgtta
    12901 tgaataatgt attgttagag gttaaagatt tagaaacatc attaaaaata aataatgaat
    12961 ggttagcaac tgttgaaaat atttcttttg aattatctaa aggagaagtt ttgggtatag
    13021 taggggaatc tggttgcggt aagtccatat taagtaagtc aattattaaa ttattaccag
    13081 aaaagatatc taaactaagt aatggagaag ttatatttga tggtaaacga atcgatacgc
    13141 tcaatgagaa gcaattgcca gatattcgag gaaatgatat tgctatgatt tttcaagaac
    13201 ctatgactgc tttaaatcct gtatttacca taaaaaatca acttgtggaa tctataaaat
    13261 cacataaaaa aatttctaaa aaagaagcaa ataaattagc aaaagattta ctaaaaaaag
    13321 ttggaattgc tagacaagat gaaatattaa atagctatcc tcatcaatta tctggtggta
    13381 tgagacaaag agtaatgatt gcaatggcca tttcatgttc tcctaaatta ttaattgctg
    13441 atgaacctac aacagcattg gatgtcacga ttcaagcgca aatattagac ttattaaaag
    13501 aattgcaaaa ggaaacgcaa atggcaatta tgatgattac acatgatttg agtgtagttg
    13561 ctgagttttg cgataaagtc ttagttatgt atgcaggtca aattgtagaa tttggaggca
    13621 taaaagaaat actacacaat ccgaaacatc cttataccca aaaattatta tcaacaattc
    13681 caaaacttaa agaagagcag aaacgacttg aaacgataga aggaattgtg ccatcaatcc
    13741 aagcatttca cgttaataag tgcagatttg caaatagatg taacaaaaaa ctggatattt
    13801 gtaataatca atctcctaaa atgcatgttt gtgaagatgt cattgtacgt tgtcatttgt
    13861 acaaaaatga atataaggag atataagaat ggaaaatatt ttagaagtca accaaataaa
    13921 aaaatactac aaaattaaaa ctggattatt acaaaaaact cagtacgtta aagctgttga
    13981 tgacgtatcg ttttcaataa aaaaaggaca aacttttgga ttagtaggag aatcgggttg
    14041 tggtaagtca acgttaggta aagtgattat caggcttgaa gatgcaactt caggctcaat
    14101 aattgttaat ggtgaagata taacaagatt acaaggtaaa aaactcagaa aatcacgaca
    14161 acaatatcag atgatatttc aagatccgta tgcatcattg aatccgatgc aaatggttgg
    14221 agatatcatt tcagaaccta ttttaaatta taaaaaattg ccaaaagaag aaataaaaaa
    14281 agaagtacta tatttattaa aatgtgttgg cctaagtgaa gatgcatatt ataaatatgc
    14341 acatgaattt tcaggtggac agagacaaag agtgggaatt gcaagagcat tggctttgcg
    14401 tccgagttta attgttgctg atgagcctgt aagtgcatta gatgtatctg ttcaatctca
    14461 agtactgaat ttattaaaag atttacaaga acaatttaac ttaagctatt tatttatcgc
    14521 acatgattta agtgtagtaa aacatataag tgatgtcatt ggagttatgt atttaggtca
    14581 tatagttgaa atcgcatctg ataaagaaat ttatgaaaat cccaaacatc catatacaaa
    14641 agcgttgatt tcatcaatac cacaaattga taaacataat aacaatagaa ttatattaaa
    14701 aggagaatta ccttcgccaa gtaatccgcc gcaaggttgt ccttttcata caagatgtcc
    14761 aattgctcaa gatatgtgca aaaaaagtat gccagaatta aaggacattg gtaatgaaca
    14821 tcaagttgct tgtttctacg tagataaagt aggtgattta aatgattaaa ttaatactta
    14881 aaagattagg gttaatgatc ccgttactaa ttttaatctc aatagttgta ttttcattag
    14941 caatcataca accaggagat ccattttcag atctacaaaa cggtaaaata aaacaagaag
    15001 caataaatgc acaaagggaa aaattaggtc tcaacgactc aataccacat caatacatta
    15061 gatgggtcaa tcatgttata cacggtgatt taggtgagtc aatcaaatat aaaaggccgg
    15121 taattgatgt tattgaggaa agaattccaa atacaatatt actcggtgct atgtcattaa
    15181 ttattactta tattatctca tttgctttag gaataacgtc aggtagatat tcttacagtt
    15241 tgacggatta tactgtgcaa atatttaatt atttgatgtt agccattcca tcttttattg
    15301 cgggagtatt tgcaattttt attttttctt ttgaattaca atggttcccg tttcaaggtt
    15361 ctgttgatat taaccttaaa gaaggtactt ttgaatatta tatgagtaaa atatatcaca
    15421 catttttacc tgcattcact ttaggtttat tatctactgc tggttatatt caatatttac
    15481 gtaatgatat tattgaaaat tctaaaaaag attatgtatt gacggcaagg tcaaaaggat
    15541 tatctatgaa taaaatttat aataaacata tattgagaaa ttctctaata cctattatta
    15601 catttttagg tgctgatatt gtaagtattt taggtggagc cgtgattacg gagactatct
    15661 tttcatataa cggtatcggt aaattatttt tagaatcggt aataggccaa gactatccat
    15721 taatgatggc attaacgtta tttttctcat ttttaggttt actgggtaat ttgatttctg
    15781 atattactta tggatttata gatccaagaa ttagaagtaa ctagggggga ttagaatgca
    15841 aaataagtca a
//
LOCUS       DAKTGS010000058        15845 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.058, whole genome shotgun sequence.
ACCESSION   DAKTGS010000058 DAKTGS010000000
VERSION     DAKTGS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.058"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(223..1125)
                     /gene="cvfB"
                     /locus_tag="O0286_001488"
     CDS             complement(223..1125)
                     /gene="cvfB"
                     /locus_tag="O0286_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCY0169871.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1272..2873
                     /locus_tag="O0286_001489"
     CDS             1272..2873
                     /locus_tag="O0286_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0169872.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3733..4938
                     /locus_tag="O0286_001490"
     CDS             3733..4938
                     /locus_tag="O0286_001490"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0169873.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            5002..5991
                     /locus_tag="O0286_001491"
     CDS             5002..5991
                     /locus_tag="O0286_001491"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCY0169874.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5993..6880
                     /locus_tag="O0286_001492"
     CDS             5993..6880
                     /locus_tag="O0286_001492"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCY0169875.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6877..7599
                     /locus_tag="O0286_001493"
     CDS             6877..7599
                     /locus_tag="O0286_001493"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCY0169876.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7626..8345
                     /gene="dapD"
                     /locus_tag="O0286_001494"
     CDS             7626..8345
                     /gene="dapD"
                     /locus_tag="O0286_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCY0169877.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8488..9639
                     /locus_tag="O0286_001495"
     CDS             8488..9639
                     /locus_tag="O0286_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0169878.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9644..10729
                     /locus_tag="O0286_001496"
     CDS             9644..10729
                     /locus_tag="O0286_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0169879.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10719..11984
                     /gene="lysA"
                     /locus_tag="O0286_001497"
     CDS             10719..11984
                     /gene="lysA"
                     /locus_tag="O0286_001497"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCY0169880.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12224..12625)
                     /gene="msa"
                     /locus_tag="O0286_001498"
     CDS             complement(12224..12625)
                     /gene="msa"
                     /locus_tag="O0286_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCY0169881.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
     gene            complement(12822..13022)
                     /gene="cspA"
                     /locus_tag="O0286_001499"
     CDS             complement(12822..13022)
                     /gene="cspA"
                     /locus_tag="O0286_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCY0169882.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            complement(13193..13501)
                     /locus_tag="O0286_001500"
     CDS             complement(13193..13501)
                     /locus_tag="O0286_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCY0169883.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            13664..13933
                     /locus_tag="O0286_001501"
     CDS             13664..13933
                     /locus_tag="O0286_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCY0169884.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            13952..14581
                     /locus_tag="O0286_001502"
     CDS             13952..14581
                     /locus_tag="O0286_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCY0169885.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            14613..15749
                     /locus_tag="O0286_001503"
     CDS             14613..15749
                     /locus_tag="O0286_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCY0169886.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
BASE COUNT         5655 a         2492 c         2760 g         4938 t
ORIGIN      
        1 caacgagctg tacattataa aatacatatc aaaaaagctg atttccatca aataattaat
       61 agaaatcagc ttttttacat tgcctaagaa cttaatgtcc cagcctattc gtttgaatta
      121 tttaattttt tactttgaaa ggaatgttta aaaaactact tcatctgtcc gatttaatat
      181 tgaataaaaa aaacacgcat cctacgacac gtgtaaaaat gattattctt ttgagtccat
      241 tcgactccaa ccttttttag ttaaagcgat tttacctgtt tctatattaa taatcttctg
      301 tttatataag tgaccgattg cacgtttgaa tgaaccttta ctcatattga atacttcttt
      361 aatcgcttca gggcttgatt tgtcccagaa tggtaattca ccatcatatt caactagtaa
      421 atcaaagatg acttggccgt catcgtctaa acgttcatgt gcaagtggta aaaatgaacc
      481 atttaactca cctttatcat tatgcccgat aattctaact tgaacagatt cacctaatct
      541 tggttcagct ttacgttctg attcatgtac gaaaattttg taacctgatt cgcttaataa
      601 aaagctacca attcgtaata cgcggtaagg tttggcttca atgacttcgt tttttaaatt
      661 atcgtcgtgt acaggtgtaa acatattttc tacaacagat tcactcgcta aacgtccata
      721 catatgattc tcacggtcaa ttcgtaatgt aactagcaaa taatcaccag gttgtggcca
      781 tagtgatttc acttttggta aatcttccca tggtactaac acttcacggg gtaatccaac
      841 atctatacgt gccccatcgc gatccgtttt aagtacttta gcaaagtcat atttatcttt
      901 cgtaatatca ggcatatttt gagttgcaaa taattcacct gaacggtttg gataaatgaa
      961 gaaactatat tcttcaccta cttctaattc atcatcatcg ttcatttctg attggtttaa
     1021 ctttacgttt tcaccgtttg gtccttttaa aaggtaagtt gaaccttgta accctactac
     1081 ttcaaggaat tctatagaac ctactatatc tttgtctaat gccatgttat tctccttcga
     1141 ttcttgtatt ctttattatt ataacatgtg tgagtcattc tatcattaca acttatatga
     1201 ggtcgaaact gtgacgataa tatgatataa ttcttccgtt agaatatcga atgaaaaaaa
     1261 ggagaaaatg catgttacaa gtaactgatg tgagtttacg ttttggagat cgtaaactat
     1321 ttgaagatgt aaatattaaa tttacagaag gtaattgtta tggattaatt ggtgcgaatg
     1381 gtgcaggtaa atcaacattc ttaaaaatat tatctggtga attagattct caaacaggac
     1441 atgtttcatt agggaaaaat gaacgtctag ctgttttaaa acaggaccac tatgcttatg
     1501 aagatgaacg cgtgcttgat gttgtaatta aaggtcacga acgtctttat gaggttatga
     1561 aagaaaaaga tgaaatctat atgaagccag atttcagtga tgaagatggt atccgtgctg
     1621 ctgaacttga aggtgaattt gcagaaatga atggttggaa tgctgaagct gatgctgcta
     1681 accttttatc tggtttaggt atcgatccaa ctttacacga taaaaaaatg gctgaattag
     1741 aaaacaacca aaaaattaaa gtattattag cgcaaagttt attcggtgaa ccagacgtac
     1801 tattactgga tgagcctact aacggtctcg atattccagc aatcagttgg ttagaagatt
     1861 tcttaattaa ctttgataat actgttatcg tagtatcgca tgaccgtcat ttcttaaata
     1921 atgtatgtac tcatatcgct gatttagact tcggtaaaat taaagtttat gttggtaact
     1981 atgatttttg gtatcaatct agtcagttag ctcaaaagat ggctcaagaa caaaacaaga
     2041 aaaaagaaga aaaaatgaaa gagttacagg actttattgc tcgtttctca gctaacgctt
     2101 ctaaatctaa acaagcaaca agtcgtaaaa aacaactcga gaaaattgaa ttagatgata
     2161 ttcaaccatc atcaagaaga tatcctttcg ttaaattcac acctgagcgc gaaatcggta
     2221 atgacttact aatcgttcaa aatctatcta aaacgattga cggtgaaaaa gtattagata
     2281 atatttcatt cacaatgaat ccaaatgata aagcaatttt aattggggat agtgaaattg
     2341 cgaaaaccac attgcttaaa atattagccg gtgaaatgga accagacgaa ggttcatata
     2401 aatggggtgt aacaacgtca ttaagttact tccctaaaga taactcagaa ttctttgagg
     2461 gcgttaatat gaaccttgta gattggttaa gacaatacgc accagaagat gagcaaactg
     2521 aaacattttt acgcggcttc ttaggccgta tgctatttag tggagaagaa gttaagaaaa
     2581 aagctagtgt actttcaggt ggagaaaaag tacgttgtat gctaagtaaa atgatgttat
     2641 caagtgcaaa cgttctttta cttgatgaac caacaaacca cttagactta gaaagtatta
     2701 ctgctgttaa tgatggactt aaatcattca aaggttctat tatctttact tcatatgact
     2761 ttgaatttat caatacaatc gcaaaccgtg ttatcgattt aaataaacaa ggtggcgttt
     2821 caaaagaaat tccatatgaa gaatatttac aagaaatcgg cgttttaaaa taaacataat
     2881 atgtagaatt tcttttcgaa aatctctgta ttgaggcccg accaacttgc acattattgt
     2941 aagttggctt actgtcacct tctatattgg gacttattcc gcaacttgta ttgtttgtag
     3001 aaattaggaa tccaatttct ctatgtgttg caccgacaag gattgaaaaa agctagctac
     3061 aaagtcattt tcattcagtc aactactgtc aatataacat tgcagggcct agggcattga
     3121 ttcatgtccg agactatatt caatttaata gacattaact gggcgtctcg atgctaatat
     3181 ttatagcatc gaggtgtcct ttttatttta ttttttaaca catcagcata gtgaacaccc
     3241 cttctctaaa ttacaaatac agttatcaac gcattaaaag agtacattta tttaatgtgc
     3301 atattgatta tgctataagt attttgaatt ttatttttca aaaagtctca ttatcaccac
     3361 cttttaaaat ataagcaacg ctataacttg aattttcagg ttattccaaa aaatctaaat
     3421 tatcggttta cattcatatt tgtcatatgt ataatgaaca cataccaatt taataatatt
     3481 ttgatgaggc gcatcaatca tgagtaaagt ttagattact gtctgctaac agctaaattt
     3541 gaaagggtgc gatgccgaag caattataat agcagttata atttgttgga ctttttggtt
     3601 aagagctgag agtttgtcat tatttaaaaa taatggagtg catcacttgt atatagatta
     3661 agagcaagtt cgcattccga acttgttctt tttttattat tgtgtgccct tcctaacaat
     3721 taggaggatt atatggtaac aagaagtgtg ttgaaatttg gcggatcatc cgtcagtgat
     3781 tttacaaaaa taaaaaggat cgctgaaatg ttaaaggagc gagtcaatca agatgaacaa
     3841 ttaattgtcg ttgtaagtgc tatgggtaac acaacagatc aattaatgac gaatgtatca
     3901 accttgacta aagcaccaaa acaacaagaa ctggcattat tattgacaac cggagagcaa
     3961 caaactgtat cttatttatc aatggtatta aatgatatcg gtatgaatgc caaagcaatg
     4021 actggctatc aagcgggtat taaaaccatt ggccatcatt taaaaagtaa aattgctcaa
     4081 attaatcctc aaacatttga acaagccttt caagaaaacg atattttagt agttgctgga
     4141 tttcaaggca tcaatgaaca tcaggaatta acaactttag gcagaggtgg ttctgatacg
     4201 accgctgtgg cacttgctgt tagtaatcaa ataccttgtg aaatttatac cgacgttgat
     4261 ggtgtgtatg ccactgaccc aagactttta ccaaaggcta aacgactaga catcgtctca
     4321 tatgaagaaa tgatggaaat gagcgcttta ggtgctggtg tacttgaaac aagaagtgtt
     4381 gaattagcta aaaactataa tatcccttta tatttaggaa aaactttatc gaacgtgaaa
     4441 ggaacatgga ttatatcaaa tgaagaaata ttagagaaaa aagcagttac tggtgtggct
     4501 ttggataaac atatgatgca tgtaacaatt agttatcccc tacctgacaa tcagctactt
     4561 acccaactat ttacggaact tgaagaaggt gctgtaaatg ttgatatgat ttcacaaatc
     4621 gtcaacttgg atgggctaca actatccttc acgattaaag atagtgattt tcatcaaatt
     4681 tctatgattc ttgaaacatt aaagaatcaa tatgaagcat tagcttataa aatcaatgag
     4741 cattatgtca aaatttcatt aattggctca ggcatgcgtg atatgtcagg tgtggcatca
     4801 aaagcatttt tgacattaat tgaaaataat atacctttct accaaacaac aacatctgaa
     4861 ataagtattt catacgtcat tgatgatttt aatgggcaac aagcggtaga aaaactatat
     4921 gacgcattta acatttaatg gtaaaatgat tgttaaaata ttctaaaaat tggaaattat
     4981 tataaaatgg agtgacaagt tatgacaaag ttagcagttg tgggtgcaac aggattagta
     5041 ggtacaaaaa tgttggagac attaaatcgt aaaaatattc ctttcgatga attagtatta
     5101 ttttcatcag cacgttctgc agggcaagaa gttgaatttc aaggaaaaac atatacagtt
     5161 caagaattaa ctgatgctcg tgcaagtgaa catttcgatt atgtattaat gagtgctggt
     5221 ggcggtacaa gcgaacactt tgccccactt tttgaaaaag ctggtgcaat cgttatagac
     5281 aattcaagtc aatggcgtat ggcagaagat attgatttaa tcgttccgga agtcaatgaa
     5341 cctacattta caagaggtat cattgccaat ccaaactgct ctacgattca atctgttgta
     5401 cctctaaaag tattgcaaga tgcttatggt ttaaaacgag tggcatatac aacatatcaa
     5461 gctgtatcag gttcagggat gaaaggtaag aaagatttag ctgaaggtgt aaatggtaaa
     5521 gcaccagaag catatccaca tccaatttat aataatgtgt taccgcatat tgatgtgttt
     5581 ttagaaaacg gatatacaaa agaagaacaa aaaatgattg atgagacgag aaaaatttta
     5641 aatgcgccag acttaaaagt aacagcaaca tgcgcacgtg tgcctgttca agatagtcat
     5701 agtgttgaaa ttgatgtaac gcttgacaaa gaaacaacag cagaagatat taaagcgtta
     5761 tttgatcaag atgaccgcgt tgttttagta gacaatccag agaacaatga atatccaatg
     5821 gcaatcaatt ctactaataa agatgaagtg tttgttggcc gtatacgtag agatgattca
     5881 ttagaaaata ctttccatgt atggtgtaca tcagacaatt tattaaaagg tgctgcatta
     5941 aatgctgtac aagtattgga acaagttatg cgtttaaaag gagcgaatta aaatgacaca
     6001 tttatttgag ggtgttggcg ttgcacttac aacccctttt acaaataaca aagttaatat
     6061 tgaagctttg aaaacacacg ttaatttttt actagaaaat aatgcccaag caatcatcgt
     6121 taatggaact actgctgaga gccctacttt aacaacagat gaaaaagaac gcattctaaa
     6181 aacagttatt gatcttgtag ataaacgtgt tcctgtcata gcaggaactg gcactaatga
     6241 tactgaaaag tcaatccaag cttcaatcca agctaaagcc ttaggggctg atgcaattat
     6301 gttaattacg ccctactaca acaaaacgaa ccaacgtggt ttagtcaaac actttgaagc
     6361 gattgcagat gctgtgaaat taccagtcgt gctgtacaat gttccttcaa gaacgaacat
     6421 gacaattgaa ccagaaactg tagaaatatt aagtcaacat ccttatatag ttgctttaaa
     6481 agatgctacg aatgattttg agtatttaga agaagtgaaa aagcgcattg atacaaattc
     6541 atttgcatta tatagtggca atgatgacaa cgtcgtcgaa tactatcaac gtggcggtca
     6601 aggggttatc tctgttattg ccaatgtcat tcctaaagaa tttcaagcgt tatacgatgc
     6661 tcaacaaagt ggattagata ttcaagatca atttaaacca atcggcacac tgttatcagc
     6721 tttatcagtt gatattaacc caattcctat taaagctcta acaagttatt taggatttgg
     6781 aaattatgaa ttacgtctac cattggttag cctagaagat acagatacta aagtgcttcg
     6841 tgaaacatat gacacattta aagcgggtga aaatgagtga aaatattact aattggctat
     6901 ggcgcaatga atcaacgcgt tgctagatta gcagaagaaa aaggacatga aatcgttggg
     6961 gtcattgaaa atacaccgaa agcaacaacg ccatatcaac aatatcaaca tattgcagat
     7021 gttaaaggtg ccgatgttgc aatagatttt tcaaatccaa atctgctttt ccctttatta
     7081 gatgaagatt ttcatttgcc attagttgtg gcaacaactg gcgagaaaga aaaactactt
     7141 aataagttag atgaattgag tcaaaatatg cctgtgtttt tcagcgcgaa catgagttat
     7201 ggcgttcatg cattgactaa aattttagca gctgctgttc ccctacttga tgatttcgac
     7261 atcgaattga ctgaggcaca tcataataaa aaagtagatg caccaagtgg tacgttagaa
     7321 aaattgtatg atgtgatcgt atctttgaaa gaaaatgtaa cacctgtgta tgatagacat
     7381 gaattaaatg aaaaacgcca gccacaagat attggtatac attctattcg tggaggtacg
     7441 attgtcggtg aacatgaagt tctatttgct ggcactgatg aaacgattca aatcacgcat
     7501 cgtgcacaat caaaagatat ttttgcgaat ggtgcaatac aagcagcaga acgcttagtt
     7561 aataaaccaa acggctttta tacgtttgac aacttataaa catattaaag gagatcgatt
     7621 attttatggt acaacattta acagctgaag aaattattca atatataagt gatgctaaaa
     7681 agtctacacc aataaaagta tatttaaatg gtaattttga aggcatcaca tatccagaaa
     7741 gttttaaagt atttggttca gaacaatcta aagtaatctt ttgtgaagcg gatgattgga
     7801 aaccttttta cgaagcatat ggtagtcaat tcgaagatat agaaattgaa atggatcgtc
     7861 gcaattctgc tattccatta aaagacttaa caaatacgaa tgcacgaatt gaaccaggtg
     7921 cgtttattag agaacaagcc attattgaag atggtgctgt cgttatgatg ggcgcaacaa
     7981 ttaatattgg cgcagtcgtt ggcgaaggta caatgattga tatgaatgct actctcggtg
     8041 gtcgtgctac aactggtaaa aatgtacatg taggggctgg cgcagtatta gcaggtgtga
     8101 ttgaaccccc tagtgcttca ccggttataa tcgaggatga tgtattaatc ggtgcaaatg
     8161 cagttatttt agaaggtgta cgtgttggta aaggtgctat tgttgcagct ggcgcgattg
     8221 tgacacaaga tgtaccagct ggtgcagttg ttgctggtac acctgcaaaa gtgattaagc
     8281 aagcttctga agtacaagat actaaaaaag agattgtagc agcattaaga aaactgaatg
     8341 actagtgacg tcaacgtata ataatttcaa ggttgagata cgattatgtc tcaaccttat
     8401 tgtttagcat tgaataaact tatgatcaca aatttcgaaa tacgttacac tatttatata
     8461 gtaatttaac acaaaggatt gataattatg aatgaattag aatttgttac gaaacatcgc
     8521 cgtcatttac atcaacatcc tgaattaagc ttacatgaat ttgaaacaac tgcttatatt
     8581 aaagcgtttt tagatagttt aaatattaaa tacgattgcc cattggaaac tggcgtcatt
     8641 gcatacttag aaggtaatgg ctcacatacg atagcgtata gagctgatat tgatgcgtta
     8701 cctattttag aggaaaatga tgtgccttat cgcagtcaat ctgatcatgt gatgcatgct
     8761 tgtggacatg atggtcatac aactgcatta atgctttttg tacaacgttg caaagacatg
     8821 caagatgcag gtcaattacc gcaaaatgtc gttttcattt tccaacctgc agaagaaact
     8881 ggtggcggtg caaatcgatt aataaaagcc ggtgcctttg ataagtatcc aattgaagcg
     8941 gtatttggta ttcatgttaa cccatttgct gatgaaggca ttgcagtgat aagagatgaa
     9001 gaaattacgg ccagcgcaac agagtatcgc tttttcttaa caggcctgtc aagtcatgtt
     9061 gctgataaag aacaaggtca ttcttgtggt gaagcattac aacatgtatt aactcaaata
     9121 tcacaaattc aacaatttca ccttaacggt ttgaaacgaa atattgttca tattggtcat
     9181 tttaaagctg gtgaagcgat taacactgta ccaagtaatg gctatttaga aggtactatt
     9241 cgtacatatg atattgatga tttaacaatc gttaaaaatc aaatgcacaa gatagcagaa
     9301 agtgtcaagc ttctgtttaa tgtagattgt gaagttaaat ttgcagaagg ttatccccct
     9361 acaatcaata gtccgaaatt acgtactcaa atagaggacg ccttaataaa agctgattta
     9421 aatgtctatg acaaaccaac gccattctta tttggggaag attttagttt ttatggtcaa
     9481 caactagctc cagcttactt tgtttttata ggaacacgaa atgaagataa aggttttgta
     9541 actggtttgc acacatcaca tttaaatttt gatgaaaaag tgttaataaa cgtggttaat
     9601 ttttacgaaa atttattaaa taattacaaa gaggtgtaat acattgacag caacatggtc
     9661 tgtaaataag aaaatatttt tacaaaatgc aatcacagtc aaaaacaatc agccattaat
     9721 ggcagttgtt aaaaataatg catatcacta tgacctagaa tttgctgtaa ctcagtttat
     9781 ccatgcaggt atagatacat ttagcacaac atcactacga gaagcaattc aaattagaca
     9841 acttgctcca gatgcaacaa tctttttaat gaatgcagtt tacgagtttg atttagtccg
     9901 tgaacatcaa atacatatga ctttgccgtc gttgacatat tactataacc ataaaaatga
     9961 tttagctggt attcatgttc acttagaatt tgaaaattta ttacatcggt ctggatttaa
    10021 agatttaaac gaaattaaag aagtattgaa agatcaccat cataatcaaa atgcaaaaat
    10081 gattattagt ggtttatgga cccattttgg atatgctgat gaattcgatg tgtcagatta
    10141 taatgttgaa cgttcacaat ggatggaaat tgttgaagca cttttatctg aaggttatca
    10201 gttcgaccta atccatgctc aaaatagtgc gagtttttat cgggaaggac aaatattact
    10261 accccaccat acacatgcgc gtgtaggtat tgcgttatac ggttcaagac catatagttc
    10321 actgaatcaa catgatatag ttcagtcatt aactttaaaa gcacatgtta ttcaagtgcg
    10381 cgaagtacaa gctggtgatt attgcggtta tagctttgcc tttgaagtga ctaaaaacaa
    10441 tacaaaatta gctgtagttg atatcggtta tggcgatgga attttaagaa ctcgtgctaa
    10501 acatgaagca cttatcaatg gtaaacgcta cccgatacgt gcattaatga tgagccatat
    10561 gtttgttgaa gtagatggca atgtacatgc acaagatgaa gttattcttt ataataatga
    10621 tatccgcatc gatgaatata cctttaaagg tgttggtgca aattctgaac aattaagtgc
    10681 tatgaatcat gattctttaa aaaaggagta catttcaaat gactgttaaa tataatcaaa
    10741 atggcgaatt aacaatggat ggtattagtt taaaaacgat tgcacaaagc tttggtacac
    10801 ctaccattgt ttatgatgaa ctacaaatta gagaacagat gcgccgttac catcgcgcat
    10861 ttaaagatag tggattaaaa tacaatattt catacgcctc aaaggcattt acttgcattc
    10921 aaatggtcaa acttgtagct gaggaagatt tacagttaga tgttgtttct gaaggtgaat
    10981 tatatacagc tttagaagca ggttttgaac cgagtcgcat ccatttccat ggtaacaata
    11041 aaacgaaaca tgaaattagg tatgctttag aaaataatat cggttatttt gttatagatt
    11101 cattagaaga aattgaatta atagaccgct atgctaatga tacggttcaa gttgtattac
    11161 gagttaatcc aggtgttgaa gcacatacac acgaatttat tcaaactggg caagaagata
    11221 gtaagtttgg attatcaatt caatatggct tagctaaaaa agcaattgac aaagtccaac
    11281 aatctaaaca cttaaaatta aaaggtgtac attgtcatat tggttcacag attgaaggta
    11341 cagaagcatt tattgaaact gctaaaattg ttttacgttg gcttaaagag caaggcattc
    11401 aagttgaatt attaaacctt ggtggtggct ttggtattaa atatgttgaa ggtgacgaaa
    11461 gtttccctat cgaaagtggt attaaagata ttacagacgc aataaaatcc gaaattaaag
    11521 ttctaggtat agatgcacca gaaataggta ttgaaccggg acgatcaatt gtaggtgaag
    11581 ctggcgttac tttatatgaa gttggaacca ttaaagaaat tccagagatt aataaatatg
    11641 tttcaatcga tggcggtatg agtgatcata tcagaactgc actttatgac gcaaagtatc
    11701 aagcattgct tgttaataga aatgaagaag cagatgacag tgtaactata gctggaaaat
    11761 tatgtgagtc tggtgatatc attattaaag acgctaaatt accttcatca gtcaaacgtg
    11821 gagactatct tgctatatta tcaactggtg catatcatta ctctatggca tccaattaca
    11881 atcaaatgca aaagccttct gtgtttttct taaaagatgg caaagcacgt gaagttataa
    11941 agcgacaatc gttaagacaa ctcattatta atgatacaaa ataaaaataa acaaagtaat
    12001 ccccgaagca cagaaaatta tactgtgact cggggaattt ttatgcatta tagatgactg
    12061 tagaaaacat gcttggacat atactctaaa aaaggaagaa cagcacattt ttagttagaa
    12121 agtatttatt tctttttgtc attaatcctt tatatttagt tagccctatt caactaattt
    12181 aaagcatcat atacatacca cactttaaac aagcatcatg aaattagatt ttatgcttta
    12241 cttgcttttt caaataatat aaaacgataa gaattaacaa caaatataat gtataaaaca
    12301 gaatttcatc aaatccaaaa tctttacttg taattaatac atcaatatct tctaattcca
    12361 tcatctctgc tttaatataa ttattaaact ttggcaaagt cattaaatgt acattgtaaa
    12421 tatagtaata aaagtcgata aatatgatgt taaaaaatat gtatgtttta tcaatattag
    12481 ttttgaaaaa tagtatccca gaaatcatta tcggaatcac taatacaatc atcattgctg
    12541 ctaaaatatt aatatttaat ccaattgcag ataatacacc gaaaactaat aaattagcaa
    12601 ctaaactaag gataagatat ttcataaaat cacctcgtcg ttgtacacca taattatatt
    12661 atataaatat atttttctat tgcaaacaat gttggtgata taaaaaaaga gttctcaatt
    12721 tggttgagaa actcatcgcg ttaagtattt gtaataagca tataaaaaca ccttacttct
    12781 tggtaaggtg ttagttaaaa tcaatgattc aaatctaaga attatagttt aacaacgttt
    12841 gcagcttgtg gaccgcggtc gccttcaact acttcaaact caacagcttg accttcttct
    12901 aatgatttgt aaccatcttg gttaattgct gaaaaatgta cgaatacgtc attttctcct
    12961 tcaacttcga taaagccgaa tcctttttca gcgttaaacc atttaactgt accttgtttc
    13021 ataatctgaa acctccaaga ctaaaattca ttcaatatgc ttattcgcat attacaaaaa
    13081 atacatgcaa atttcactca aatattcttt gcaatatgga ataatcatct gcttaacttg
    13141 tattatagtg cagttattac caaattgcaa tacaataata aaaaatattc atttaatcaa
    13201 ttattggtag tttataatat acttcattat tttgtaaaac aatgaaacta taaaatattt
    13261 gtaaatcctc ttcacaatct tcgcaatagt tcatgtcaca attattataa aatgcataaa
    13321 tattatattt gcctgtgaca tagttatcga actgaacttt acattgatta atcaattgtt
    13381 gatagtgctt aaacatgtca tcaaaatctt gatattgata tttttcaaca atgttttctg
    13441 gccagtcact gaataaccac catccctcat aatcggctct aattttggta actgtccaca
    13501 ttattaaaga ccccttccat acttcaaaaa ctctatactt atttttatac ctttatcctc
    13561 tgaaaatcaa gtttgacgac ttgaaacaaa caaagtagta actatagtaa ttattagttt
    13621 ttttaagtaa aactacgtat aataacatta tgaggtggta aaaatgagac atatacattt
    13681 acaagtattc ggacgcgttc aaggcgtcgg atttagatat tttacacaac gcattgcaat
    13741 gaactataac attgtcggta ctgttcaaaa tgtagatgac tatgtagaga tatatgcaca
    13801 aggggatgac gcagatatag agagatttat tcaaggtgta attgaaggtg cctcaccagc
    13861 atcaaatgta acaagccatc aacttgaaga gttagaacta aatcaaaaat tatcggattt
    13921 tcgatcaata taaaatgcta aataaaggaa gatgacagtg agatataata tttctcatat
    13981 atttggggtg ttagtgggaa ttcctgtagc gtttttaaca agcatatttg ggatgattgc
    14041 acttgatgta tcttttttaa ttgatatgtc tattggtatt gttggctttt taatgacata
    14101 cctaccgata caaaaactca cttcacgcaa atatttaaac gaaattggtt tgactagaaa
    14161 agactatcgc tatattcgaa atcagttaaa tcatacacac caaaaactta gaggtatttt
    14221 aaaaacgtat gtcaatataa gatcaattaa agattttagg cagattaatg atatatacca
    14281 aatttcacgt tctatttata cgacagttag acagagacct gcatcatttt ataaagttga
    14341 aggctttttt tattctcata ttgataatgc tttaaatttg gttgatgcat atacacgtct
    14401 agcaaaaatg cccaaaaaat caattaatga acagcaaaag ttagaacaaa cacgaattac
    14461 tttggatgag gtcaaacgaa cattaatcgc tgatttaaag cgtctcaacg aagatgatta
    14521 tgaacgttta gatattgaaa tggaattaaa taagttacat caaaaacatc atcaagattg
    14581 atgtaaaata gcaactattg gagagatgat tgatgactga aaataaaagt ttcaaagaaa
    14641 gccatccact agatgatttt ataagcgata aagaattatc gaatactact attcaaaaag
    14701 aaaagttaac aattgaacaa caaaaacagg tagacacaat cagtaaacaa attaaccctt
    14761 tagacaatga aggtttatta gcgtttggtt ctgatttaca gaaacaaatg tctcaatttt
    14821 cacatcaaat gttggatgaa gtacaaagta aagatgttgg tcctattgga gatactttgt
    14881 cagatctaat gtcaaaacta aagtcagtta atccaaatga gttaaatact gataaaccat
    14941 ctatgttaaa aagaattttt agcagagcaa agtcgtctat caatgaaatc ttttcaagaa
    15001 tgcaatcagt tagtgctcaa gtcgatcgca taacgattca actgcagaaa catcaaacac
    15061 atttaacaag agatattgaa ttattagata cgctatatga taaaaacaaa caatactttg
    15121 atgacttatc attgcatatc attgctgcac agcaaaaaaa gttgcaatta gaaaatgaaa
    15181 agctaccaca attgcaacag caagcgcagc aatccactaa tcaaatggat attcaacaag
    15241 ttgcagatat gcagcaattt atagatagac tagataaacg catatatgac ttacagcttt
    15301 caagacaaat agctttgcaa actgcgccac aaattcgtat gattcaaaat gttaatcaag
    15361 cacttgccga gaagatacaa agttcaattt tgacaagtat tccactatgg aaaaatcaaa
    15421 tggccattgc gcttacatta atgagacagc gtaatgcagt tgctgcacaa cgagctgtca
    15481 ctgatacaac taatgattta ttaacagcaa atgctgaaat gttgaaacaa aatgcgattg
    15541 aaactgcaac agaaaatgag cgtggcattg ttgatcttga tacattgaaa cgtacacagc
    15601 gtaacattat tgagacaatt gaagaaacat taattattca acaacacggt cgcgaagaac
    15661 gacaattagc tgaaaaagaa ttacaacaat tagaacaaga tttaaagtca catttagtga
    15721 acatcaaagg accgaataaa caatcataaa ttacgaatta gatataacaa aaaagagctt
    15781 gggacattaa gttccttaaa gttcttaggc aatgtaaaaa agctgatttc tattatttat
    15841 ttgat
//
LOCUS       DAKTGS010000059        15798 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.059, whole genome shotgun sequence.
ACCESSION   DAKTGS010000059 DAKTGS010000000
VERSION     DAKTGS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15798
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.059"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..1047)
                     /locus_tag="O0286_001504"
     CDS             complement(<1..1047)
                     /locus_tag="O0286_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCY0169887.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREKTKHNNIE
                     YNVSLFFKALDALKK"
     gene            1186..1476
                     /locus_tag="O0286_001505"
     CDS             1186..1476
                     /locus_tag="O0286_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169888.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            1476..3263
                     /locus_tag="O0286_001506"
     CDS             1476..3263
                     /locus_tag="O0286_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCY0169889.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            3497..4846
                     /locus_tag="O0286_001507"
     CDS             3497..4846
                     /locus_tag="O0286_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0169890.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            4868..6496
                     /locus_tag="O0286_001508"
     CDS             4868..6496
                     /locus_tag="O0286_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0169891.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            7018..7368
                     /locus_tag="O0286_001509"
     CDS             7018..7368
                     /locus_tag="O0286_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169892.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            7455..7766
                     /locus_tag="O0286_001510"
     CDS             7455..7766
                     /locus_tag="O0286_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCY0169893.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            7781..8287
                     /locus_tag="O0286_001511"
     CDS             7781..8287
                     /locus_tag="O0286_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0169894.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            8339..9946
                     /locus_tag="O0286_001512"
                     /pseudo
     CDS             8339..9946
                     /locus_tag="O0286_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(9937..10197)
                     /locus_tag="O0286_001513"
     CDS             complement(9937..10197)
                     /locus_tag="O0286_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0169895.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(10169..11155)
                     /locus_tag="O0286_001514"
     CDS             complement(10169..11155)
                     /locus_tag="O0286_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0169896.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            11252..13261
                     /gene="mecA"
                     /locus_tag="O0286_001515"
     CDS             11252..13261
                     /gene="mecA"
                     /locus_tag="O0286_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0169897.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(13307..13735)
                     /locus_tag="O0286_001516"
     CDS             complement(13307..13735)
                     /locus_tag="O0286_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0169898.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(13832..14575)
                     /locus_tag="O0286_001517"
     CDS             complement(13832..14575)
                     /locus_tag="O0286_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0169899.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(15412..>15798)
                     /locus_tag="O0286_001518"
     CDS             complement(15412..>15798)
                     /locus_tag="O0286_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
                     /protein_id="HCY0169900.1"
                     /translation="QLCNRTVLWNRDVGNIYTGSLYLSLISLLQNHTFQPEEKVCLFS
                     YGSGAVGEIFSGSIVKGYDKALDKEKHLNMLESREQLSVEEYETFFNRFDNQEFDFER
                     ELTQDPYSKVYLYSIEDHIRTYKIEK"
BASE COUNT         5755 a         2651 c         2402 g         4990 t
ORIGIN      
        1 tttctttaag gcatctaatg ctttaaaaaa caacgataca ttgtactcaa tattgttatg
       61 ctttgttttt tctctaggaa agcagtaggc tttataataa tcaattttaa ctttcaacat
      121 attttgttca atagataaag ttgcatttaa taacgattct tctaatccat ttttaaatct
      181 tttaatctga tcttttacgt atctatactt tttaatagat tcttctatca gtgtaattct
      241 tttgtaatta tctttttctc cttcacatgt atctacaatg tctctaataa tatataatct
      301 aacaacaggt aatcgtatat ctataagtaa aataatgaca tatgattgaa taaattcgtt
      361 taattttttc ccaaactcat aatctgtttt cttttgtcct ttaaatgcct tcaaatacaa
      421 ttctatcaaa taaaatagtt catttaaaaa agcccaagca tattcctgtt ctaatgcaac
      481 ataactaatc aattgattat taagattata aaactgaatc tcattatata aatatttcca
      541 atgattctcc aattttgtat acgatatttt ttctcctaac cgtacatatt cattttcttt
      601 ttttgactga ttatagagtg tgtctatctt tgtaactaat cttttttgcg ttttgtttat
      661 ttctgccaat gcttttgaag taatatttga atcataactc atatgtgcat ctactttctt
      721 tatcgcgagt agtgatttca attgttcttc atttataaat ggattacgtt taaaattatt
      781 attttgacta ttgatatgcg tcatgatatt taaattactg taaatcttat tgatacttct
      841 aaactcagta tcatctaata aataatcgaa atttgagttt aaatgttttt ttataaatga
      901 aactctatcc atcttttgat gttcatttaa atcgcttaaa cttcttaata acaacaaatc
      961 ttcataaacc tcaactgtta catcaaattt caaataatca actaagtttc catacttatc
     1021 aataatttct tctagcgtag gtatcaatat ataccccccc tccccatttt ttgttccgcg
     1081 tctaatcata atgcttcatt tatgcttatt atagcaatca attgaagata gtttaaccta
     1141 tatgatattg attgactcct taaaaattat tggaggaatt ttaaaatgaa taagaaatct
     1201 aagcagcaag aaaagcttta taattttata attgctaaat cttttcaaca accagttgga
     1261 agtacgttca cttatggtga attaagaaag aaatataatg tggtttgtag cacgaatgat
     1321 caacgtgaag taggacgtcg ttttgcttac tggattaagt acacgccagg attaccattt
     1381 aaaattgtag gaacaaaaaa tggcagttta ttatatcaga aaatagggat aaacccatgc
     1441 aataatagca cgccttcgaa aggaggtgat tgctaatgag ttacacattg tttgaaattg
     1501 gcccatttgc tttagattcc ttcggttgga aagaaacgat accacctaaa aaagatggtg
     1561 acagtgaaaa agtggttaga atggctagcc ctattattgt taatgcaaga ttttctgatc
     1621 caattactgg tgttgaaaag ttgattatta ctaacaacaa tggtaaaaaa gacatttttg
     1681 agtctgatat cttaacaaca cgaaacttac cttctttaat taaatatggc tacagtatta
     1741 acgagaagta tattagatca ttgagttatg ccttgcaatt gatgcgagat agattaccac
     1801 tttctgaatt atatgagggt gtaggtatac tagagacacc ttttggctac ttgatatcgc
     1861 tagataaagt tctcaaatct attcaattta atcagtcctc accttcatac cctattgtag
     1921 atagtgctta tgacttaact cctaaaggta catttgataa ttggtttaac atgtatattg
     1981 acgaggttaa aggtcatctt cttttagaat tggcagtcat atttgggatt tctgccttag
     2041 tgacaagttt tctgaagcat aaacatgaaa tcgaatttgc aggaattctc tttagtttta
     2101 caggtcaatc ctcaaccggt aagtcaaccg cagctgcttt agctgtatca gtcgctggta
     2161 accctaccaa aggtaatgaa actcttttta gaagttggaa tgccacacga aacgcacttg
     2221 aaggttattt gagtaacaat tatggcattc ctatagtatt tgatgagctt tcatctacga
     2281 cacttagaga taccacgggt ttgttatatt ccatcgctga aggtcaaggt cgtcaacgtt
     2341 caaacgttca tggagaagtt aaaacgccta agaattgggg tacttctgtc atttcaacgt
     2401 cagaatacag tatttttaat gattcagctc aaaacgatgg attacgtgta agaaccatcg
     2461 agattaatga acaattcact acaaatgcga ccaatgctga caatattaaa aaagctgtag
     2521 cactaaacta tggtcatgtt ttaccgttag tagctaaata tcttataaat cgtgaagatg
     2581 aagttattca atggttttac aaggaagtag attggtttga agctaaattg aaagatgaaa
     2641 caaacaatac aggtattcgt atgtttaagc gttatgcagt cattacaaca tctgcgaaaa
     2701 tattaggtcg tgttttatct acagatattg atatcgctaa tataagagac tactttattg
     2761 attatcatac tcatacagtc tctgaacgct cattagctga taaagctatt gatgtcatca
     2821 ttcaatttgt ggcacaaaac agaggtaagt tttcagatga aggtgctttg aaaaatatgt
     2881 ttgagaacta tggattaatc tcattaaaag atgaccatat cgaagtaaaa ataattgcga
     2941 acgtttttaa acagatgcta aacaatcatc aatttcaaga tgttaataat gttgtcaacg
     3001 ccttaagaga taaaggtttc atattagctg accgtggacg ccaaacaact aaaagaagtg
     3061 tcaaagataa tagcggtaaa aaacaatcac ttgtatttta ccatctaaaa ttagatgtag
     3121 agtttgcatc tatcctcgga ttaactaaag ataagtcgtt acttcaaaat tggacaccgt
     3181 caaatgataa caaagctgcg aaagaacttt ttaaatccgc taatgaaggg ataggccctt
     3241 caggagttca cgaagatttc taattgtctt tatcatacaa ctgtgaagca tcaaactgaa
     3301 taatcgacgt attgaagata gaacaaataa cggagtgaaa aacatggatg aacttatcga
     3361 gatattagcc gatttggtaa ttgaagaaat caatcaaaca aatgactaat atggacgccc
     3421 aacatctcat tgatgttggg cttatatatc aattcatcca taaatatata ttatcactgt
     3481 gaaaggagga gccgatatga aacaagtcat aggctattta cgtcaaagta cgatgaaaca
     3541 acaatctctt gcagcacaga aacaagctat cgaagcaata gccgaaaaac atcatattca
     3601 acatatcaac ttttatagcg acaaacaatc aggtcgcaaa gataatcgta gtgggtatcg
     3661 acaaataaca caattaattc aacaaggaca gtgtgacata ttatgctgtt atcgtcttaa
     3721 taggttgcat cgtaatctga aaaatgcatt aaaactcatc aaattatgtc aaacgtacca
     3781 tgttcatatc ttaagcgtac atgatggtta ctttgatatg gatcaagctt tcgaccgact
     3841 caagcttaat atcttcatca gtttagccga acttgaatcg gataacattg gagaacaagt
     3901 cagaaatggg cttcaagaaa aagcaaagca aggtcgattg attacaacac atgcaccttt
     3961 tggttacgaa tatcacaacg gaacattcat catcaatcaa aatgagtcac caacggtaaa
     4021 ggctgtattc aattattaca ttaaaggtca tggttataag aaaattgcac agttattaga
     4081 agaagataac acgtatatca atcgacaacc ctatcaagtt cgtaacatta tcatcaatcc
     4141 taattattgt ggtcgtgtca acaatcaata tggccaattc gacaatatgt ttccttctat
     4201 tgtttccaca agtatatatg agcaagcgca gagacttcga ttgcaaaaac aaaccaaaca
     4261 gacaccttcg gataatcaac tcaaacaaaa aatcaaatgc ccatgttgta atgcaacact
     4321 tacaaatatg accattagaa aaaagaatca tacattacgt tactacgtct gtcctaaaaa
     4381 catgaatgct tcacgctttg tctgtgattt taaaggcatc aatgcacaaa cacttgaaga
     4441 taaagtatta gaagtgtgcc gagactttta tcaaaatcaa cgcatctaca caaaaattaa
     4501 aagtgcgatt gacaaacgca tcaaaagaca aagaaacata gaaaaacatc acacattgac
     4561 tcaagaacaa ctgatagaaa agttggcaca aggcatcatt gatgcagaaa cgttcagaga
     4621 gcaaacacaa tcattacgtc aacaaccgca acgcactaca tctatcaatg ggcatcaaat
     4681 acaacacatc attcaaaata ttattcaaaa acgtttcacg ttaaacatat tgtaccccta
     4741 tattgaaacc attcacatta cgaaagataa aaatcttata ggaatctatt tcaaaaatga
     4801 accactcaat attgtcaatc aaaccatgca atcatcgatt gcataaatga agaaaggatg
     4861 taacataatg caacaactta aaacaaaacg tgtcggtatc tatgtacgtg tatcaacaga
     4921 aatgcaaagc acagaaggtt atagtatcga cggacaaatc aatcaaatca aagaatactg
     4981 tgacttccat cattttgaag ttaaagatat atacgctgac cgtggtattt caggtaaatc
     5041 tatgaacaga cctgagctcc aacgtatgtt gaaagatgca aaagaaggca atatcgactg
     5101 tgttatggta tacaaaacaa accgattagc tcgtaataca tctgatcttc tcaaaattgt
     5161 cgaagattta cacaaacaaa atgtcgaatt tttcagtttg tcagagcgta tggaagtcaa
     5221 tacttcttct ggtaaactca tgttacagat acttgcgagt ttctcagaat tcgaacgtaa
     5281 taacattgtc gagaacgtat ttatgggtca aacgagacgt gcccaagaag gctattatca
     5341 aggcaattta ccactaggtt atgacaaaat accagatagt aaacacgagc taatgattaa
     5401 ccaacatgaa gctaatattg taaaatatat attcgagtgc tatgccaaag gacatggcta
     5461 tcgtaaaatt gccaatgcat tgaatcacaa aggatatgtc actaaaaagg ggaaaccttt
     5521 cagtattagt tcaatcacat acatcttagc taaccctttc tatatcggca aaattcaatt
     5581 tgcgaaatac aaagattgga gtgaaaaacg tcgtaaaggg ctgaatgata aaccagtgat
     5641 agctgaaggt aagcattccc ccattattaa tcaagattta tgggataaag tacaaatgcg
     5701 taagaaacaa gtcagtcaaa aaccccaagt ccatggcaaa ggaacgaatc tgcttacagg
     5761 cattattcac tgtccccaat gtggcgcacc tatggcagca agcaatacca cgaatactct
     5821 taaagacggg actaagaaac gtattcgtta ctattcatgt agtaattttc ggaacaaggg
     5881 ttccaaagta tgttcggcaa acagtgtaag agctgatgtg attgaagatt atgtgatgaa
     5941 gcaaatactt gaaatagtca aaagtgataa agtcattcaa cgcgtagtaa cacacgtcaa
     6001 tcaagaaaat caagttgatg gcgctgcact tcatcacgat attgcttata agcaacaaca
     6061 atatgatgaa gtacaaatca aaataaataa cttgattaaa accatcgagg ataatccgga
     6121 cttaacatca gtaatcagac caagtattca aaaatatgaa aagcaactca atgacattac
     6181 gaatcaaatc aaccaactca aaaatcaaca aaatgaagat aagactttat ttgatgccaa
     6241 agaaatcagt aaactattac aacacatctt tcatgatatt aagcacatag aaaaatctcg
     6301 actcaaagca ttgtatctat cagtgattga tcgcattgat attaaaaaag atggtaatca
     6361 taaaaaacaa ttctatgtca cactcaaact taataacgaa atcattaaac aacttttcaa
     6421 taataaacaa ctcgacgaag tgcatctcag cacttcgtct ttatttttgc cccaaacact
     6481 atatcttact atttagctaa tagaatcatt gttataacaa taattttcac tatattcttt
     6541 cgcttatttt atctaactat tttcaagaat tgctctttat caatttcaat aacacttatc
     6601 taatgaaaca gttattgtcg taattttttc ataactatac aatgcttata ttgaaattgc
     6661 taaagaacat cccacaatga ttttttatcg ggaaatgatt gggatttgca gattcgaaga
     6721 aacagaagga ttgtgcaagc acaatcccaa aatcccgtta ctttttataa ataaataata
     6781 tatataagga agcagagaca caaccctatc atcaatactt ataaataatg agttgattgt
     6841 ttgtcaaaat aggcggaatt tcaggatttc cttggcatac caacgtttgt agcgggtttt
     6901 tatatggaat tttctcttat tcccaccggg attcgtaata aatgacaaat caaatatata
     6961 ttatctcaat gatgtatatg aactacatca ctattacact atctaggagg caataatatg
     7021 aaaaccatca ctcaagaact caaacaatat ataacccacc tattccaact atctaataac
     7081 gaagcttggg aatgtgaagc gctggaggaa gcagcagaaa atattctacc ggaacgtttt
     7141 attaatgatt ctccactggt acatctcaca ctcgagactt atacctacta taatgatgaa
     7201 ctacatgagc taagtatcta tccatttcta atgtatgcca ataaccaact catcagtgtc
     7261 ggatacttag accattttga catggacttt ctgtacctta cagatactca aaatatcatt
     7321 attgatgaac gtcatttact aaaacaagga ggtcaggacc atgaataatt ggatcatagt
     7381 tgctcaatta tccgtcacag ttatcaacga aatcatcgac atcatgaaag aaaagcagaa
     7441 gggagggaaa tagcatgaaa atcaatcgat acatcacaag aggaattaat gaaagtattc
     7501 cactagacct tcaaatctta ctttggcaca tggtagaaaa aaaagataac cagcctcata
     7561 cagattacct acacattttt aaattacaag aagatgagaa tatactctca atcacacatg
     7621 aacaagaaca acccacatac aaattggaat atcactatac aaactatgaa aaaaatcaaa
     7681 atgcattacc taagaaagtc tacgtcattc gtgaagatga cgtggacgct ttttattatg
     7741 tgatgctttt accagaagaa tactaaagga agtgacgatg atgaatacaa tcaaaagtac
     7801 gatacacaca gaagccatat ttagcgatga tgaacagcac cgctatttac tcaagaaaac
     7861 atgggatgaa aagaaagctg catgcacagt gataacgatg tatcctcatt tagacggtgt
     7921 attatcactc gatctcacaa ctgttcttat cctcaaccaa ttagccaatt cagaacgata
     7981 cggtgctgta tatcttgtaa atctattctc taatattaga acacccgaaa acctcaaaca
     8041 tatcaaaaat ccatacgatg agcacactga tattcatttg atgaaagcga ttagtgaaag
     8101 tgacacagtg attcttgctt atggtgccta tgcgaagcga ccagttgtta tcgaccgtgt
     8161 cgaacaagtg atggaaatgt taaaacctca taaaaagaaa gtaaaaaagc tcatcaatcc
     8221 agtaacaaat gaaattatgc atccactcaa ccctaaggca cgtcaaaaat ggattttgaa
     8281 atcatagcaa atacatggag gtcaatatgg aaacaaaaca aatcaacatc gttttactgt
     8341 aaatgatatt gttgaaacaa ttcctgacaa tgaattcgac gaattcagac atcaccgtgg
     8401 tgcaacttcg taccatccta aaatgatgtt aaaagtgatt ctatatgcct acacacaatc
     8461 tgtattctca ggtcgtaaaa tagaaaaaat gcttaatgat agcatccgaa tgatgtggct
     8521 atcacaaaat caaaaacctt cttataaaac aattaatcaa tttagagtaa atccaaaagt
     8581 agatgcttta ttagaatctt tatttattca attttacagt cagtgtgtaa aacaaaatct
     8641 tatagatgat aaagctattt ttattgatgg tacaaaaatt gaagcaaatg ccaatcgata
     8701 tacatttgta tggaaaaaga gtattcaaaa ccatgaatca aagatgaatg aggattctaa
     8761 agccctctac catgaattgg taaccaataa aatcataccg gaaattaaag aagatcatga
     8821 taatgaatta acaaaagaag aaatagattt gattggtagt cacttagata aagaaatcga
     8881 agatttaaac caacatatca acaatgaaaa atgtactaaa acaagaaaac aaatacgtct
     8941 caaaagaact aaaatcaaaa aatacaaaaa gcaaatcaat gattattttg agcgaaagta
     9001 tcgatacgaa tttcaaaaat ctattttaaa ggatagaaat agttattcta agacagatta
     9061 tgatgcgaca tttatgagaa tgaaagaaga tcacatgaaa aatggacaac ttaagccagg
     9121 gtataattta caaatagcaa caaattccca atttgtttta tcttataatg tgtatcaaaa
     9181 tccaacggat actagaacga tgattccatt tttaaattca attcaagaga cctacggtca
     9241 tttacctgaa tatattgtag ctgatgcagg ttatggtagt gaatcaaatt ataaggcaat
     9301 tatagatgac tttaatcgaa cgccactcat aacatatgga atgtttataa aagataaaac
     9361 taaaaaatat aaaagtgaca tctttaatac tcaaaattgg aactatgacg aaattaatga
     9421 cgaattcatt tgtccgaata ataaacggct aggttttaaa agatatgcct atcgtcatga
     9481 taagtatggt tataagcgag acttcaaatt atatgaatgt gatgattgtt cagaatgtcc
     9541 tctgaaaaat caatgtatga acttcaattc aaaaacaaac aaaaaaataa tgaagaatta
     9601 taactgggaa tattttaaat cccaaattaa caaaaagctt tcagaaccag aaacaaaaaa
     9661 tatctacagt caaagaaaaa ttgatgtgga acctgttttt ggatttatga aggctatttt
     9721 gggtttcact cggatgtctg tccgaggact caataaagtc aaaagagaac ttggttttgt
     9781 attaatggca cttaatataa gaaaagtagt agctcaacga gctgaaaata atcaaaaaat
     9841 ttataaaaaa gacaatttct atattatttc aatagaaatt gtcttttttc acttatccaa
     9901 gaactttatg tcccggactc attatgattt atttttttat tgctgacttg ccattaattc
     9961 tgcttgctct acaacttgtt ccacagccat tttttgaaga tctggtggat agccatattt
    10021 ctttaatagg cgtctaactt gaactctcat tttagcttta gcactgtctc gtttagacca
    10081 atcaacactc atgttatcct taactgtttt agttaactca tgtgcaatgg ctcttaactc
    10141 tttatcgccc aaagcttctt tagctgtttc atgtgaagct cgatatacta catacaatat
    10201 acgcaacaat acgcttgttt cgattaggtt taggcattga atcataaagt gcaatatact
    10261 taacacgttc tttaatattt gaattaaaac ctagtaaata ttgtgctgcc acattattta
    10321 tgtgctgaga ttttaaaata gagcatttta atatcgattc accataacgt atatgttcat
    10381 ggcgattcaa aatttttaaa acgtttctat cacatacttt ttcacagtca ttgtccatca
    10441 ttgttttact tatatatagt gcaggattaa accagaatat cattttaaaa acaacataaa
    10501 gctggttgaa tattaagtca tgacttttca catgtgatag ttcatgtaga ataatatatt
    10561 caatttcttt gtcattcatg gtttcgacta cgacagttgg tagtacaatt tgggatttca
    10621 ctaaaccaaa taccatcgga ttatcaatgt ttgaactata actaattgtt atatgctttt
    10681 tgtagaactg catcttactt tgacatactt taagtcgttc attaagatat gacgattcca
    10741 atgacgaact tttaataaca tcaatttgtc ggaatgcctt aatcatataa aataagcaca
    10801 acaaactacc aaatacccat atcaaaagaa tcatatacgt tatatttgag gtctcaaact
    10861 gattaacatt aattgctaag tctttcgtaa cagatgattg ttgaccatct aacatatgac
    10921 taaccgaaga agtcgtgtca gatacatttc gattcatcat atcttttgaa aatgtaaaat
    10981 tcgatatttt gtaaaatggt attaatggaa ttaacgtgga gacgagcact aataaccaaa
    11041 tcttatgtga cataatattt tgagtatatt ttatatagag cattctcact aaaaaaatta
    11101 cacatatcgt gagcaatgaa ctgattatac ttaacattaa aaaagatgat aacaccttct
    11161 acacctccat atcacaaaaa ttataacatt attttgacat aaatactaca tttgtaatat
    11221 actacaaatg tagtcttata taaggagtat attgatgaaa aagataaaaa ttgttccact
    11281 tattttaata gttgtagttg tcgggtttgg tatatatttt tatgcttcaa aagataaaga
    11341 aattaataat actattgatg caattgaaga taaaaatttc aaacaagttt ataaagatag
    11401 cagttatatt tctaaaagcg ataatggtga agtagaaatg actgaacgtc cgataaaaat
    11461 atataatagt ttaggcgtta aagatataaa cattcaggat cgtaaaataa aaaaagtatc
    11521 taaaaataaa aaacgagtag atgctcaata taaaattaaa acaaactacg gtaacattga
    11581 tcgcaacgtt caatttaatt ttgttaaaga agatggtatg tggaagttag attgggatca
    11641 tagcgtcatt attccaggaa tgcagaaaga ccaaagcata catattgaaa atttaaaatc
    11701 agaacgtggt aaaattttag accgaaacaa tgtggaattg gccaatacag gaacagcata
    11761 tgagataggc atcgttccaa agaatgtatc taaaaaagat tataaagcaa tcgctaaaga
    11821 actaagtatt tctgaagact atatcaaaca acaaatggat caaaattggg tacaagatga
    11881 taccttcgtt ccacttaaaa ccgttaaaaa aatggatgaa tatttaagtg atttcgcaaa
    11941 aaaatttcat cttacaacta atgaaacaga aagtcgtaac tatcctctag gaaaagcgac
    12001 ttcacatcta ttaggttatg ttggtcccat taactctgaa gaattaaaac aaaaagaata
    12061 taaaggctat aaagatgatg cagttattgg taaaaaggga ctcgaaaaac tttacgataa
    12121 aaagctccaa catgaagatg gctatcgtgt cacaatcgtt gacgataata gcaatacaat
    12181 cgcacataca ttaatagaga aaaagaaaaa agatggcaaa gatattcaac taactattga
    12241 tgctaaagtt caaaagagta tttataacaa catgaaaaat gattatggct caggtactgc
    12301 tatccaccct caaacaggtg aattattagc acttgtaagc acaccttcat atgacgtcta
    12361 tccatttatg tatggcatga gtaacgaaga atataataaa ttaaccgaag ataaaaaaga
    12421 acctctgctc aacaagttcc agattacaac ttcaccaggt tcaactcaaa aaatattaac
    12481 agcaatgatt gggttaaata acaaaacatt agacgataaa acaagttata aaatcgatgg
    12541 taaaggttgg caaaaagata aatcttgggg tggttacaac gttacaagat atgaagtggt
    12601 aaatggtaat atcgacttaa aacaagcaat agaatcatca gataacattt tctttgctag
    12661 agtagcactc gaattaggca gtaagaaatt tgaaaaaggc atgaaaaaac taggtgttgg
    12721 tgaagatata ccaagtgatt atccatttta taatgctcaa atttcaaaca aaaatttaga
    12781 taatgaaata ttattagctg attcaggtta cggacaaggt gaaatactga ttaacccagt
    12841 acagatcctt tcaatctata gcgcattaga aaataatggc aatattaacg cacctcactt
    12901 attaaaagac acgaaaaaca aagtttggaa gaaaaatatt atttccaaag aaaatatcaa
    12961 tctattaact gatggtatgc aacaagtcgt aaataaaaca cataaagaag atatttatag
    13021 atcttatgca aacttaattg gcaaatccgg tactgcagaa ctcaaaatga aacaaggaga
    13081 aactggcaga caaattgggt ggtttatatc atatgataaa gataatccaa acatgatgat
    13141 ggctattaat gttaaagatg tacaagataa aggaatggct agctacaatg ccaaaatctc
    13201 aggtaaagtg tatgatgagc tatatgagaa cggtaataaa aaatacgata tagatgaata
    13261 acaaaacagt gaagcaatcc gtaacgatgg ttgcttcact gttttattat gaattattaa
    13321 taagtgctgt tacttctccc ttaaatacaa tttcttcatt ttcattgtat gttgaaagtg
    13381 acactgtaac gagtccattt tcttttttta tggatttctt atttgtaatt tcagcgataa
    13441 cgtacaatgt attacctggg tatacaggtt taataaattt aacgttattc atttgtgttc
    13501 ctgctacaac ttcttctccg tatttacctt cttctaccca taatttaaat gatattgaaa
    13561 gtgtatgcat gccagatgca atgatacctt taaatctact ttgttctgct ttttctttat
    13621 ctatatgcat atattgagga tcaaaagttg ttgcaaattg gataatttct tcttctgtaa
    13681 tatgaaggct ttttgttttg aatgtttctc ctactataaa atcatcgtat ttcatatatg
    13741 tctctctttc ttattcaaat taatttttta gtatgtaaca tgttaaaggt aagtctaccg
    13801 tcactgaaac gtaagactca cctctaactt tctattgaga caaatgcacc attttatctg
    13861 cattgtctgt aaagatacca tcaactcccc aattagcaag ttggtttgca cgtgctggtt
    13921 tgtttacagt ccatacgttc aattcataac ccgcttcttt taccattttt acttttgctt
    13981 tagtaagttt ggcatcttca gtgtttacta ttttagcatt acagtaatct aaaagtgttc
    14041 tccagtcttc acgaaacgaa gttgtatgga atataactgc tctgttatat tgtggcatga
    14101 tttcttctgc aagtttaaca agcacaacat taaagcttga aatgagcact tcttgattct
    14161 gatttaagtt tgttaattgt tcttccactt gcttaaccat acttttagaa agtgctagtc
    14221 cattcggtcc agtaatacct tttaattcta catttaaatt catattatat tcatttgcta
    14281 tttttactac atcatcgaaa gttggcaaat gttcatcttt gaatttttca ccaaaccaag
    14341 atcctgcaga agcatcttta atttcatcat aattcaattc agttatttcc ccggacatat
    14401 ttgtagtccg ttctaaataa tcatcatgaa tgataatcag ttgttcatct tttgtaattg
    14461 caacatctaa ctccaaccag tttatacctt ctacttctga agcagcttta aatgatgcaa
    14521 ttgtattttc cggagcttta ctaggtaatc ctctatgtcc atatacagtt agcatattac
    14581 ctctccttgc atttttattt ttttaattaa cgtaactgta ttatcacatt aatcgcactt
    14641 ttatttccat taaaaagaga tgaatatcat aaataaagaa gtcgatagat tcgtattgat
    14701 tatggagtta atctacgtct catctcattt ttaaaaaatc atttatgtcc caagctccat
    14761 tttgtaatca agtctagttt ttctgtaccc cttatctgca attttactta ggattgcttt
    14821 taacttaccc cttatcagca attttactga gaactgcttt taacgcacct cttatctgca
    14881 attttgccta gaactgcttt taacgtacct cttatctgca attttactga gaactgcttt
    14941 taacttaccc cttatcagca attttgcatg gaattgcttt taacgtacct cttatctgca
    15001 attttactta gaactgcttt taacaaacct cttatctgca attttactta gaactgcttt
    15061 taacgtacct cttatctgta attttactga gaactgcttt taacaaacct cttatctgca
    15121 attttactta gaattgcttt tactattcct cttattagta taatctcagt aagaatgcgt
    15181 ataaaaatga aaattacaac cgattttgta agtgctgacg cctgagggaa tagtatgtgc
    15241 gagagactaa tggctcgagc cataccccta ggcaagcatg cacgtacaaa atcgtaagat
    15301 aaaaaaataa gcatatcact gtaaacttta aaaaatcagt ttagtgatat gcttatttat
    15361 ttcgagttag gatttatgtc ccaagctcat caagcacaat cggccactag tttatttctc
    15421 tatcttatat gttctgatat ggtcttctat actgtataag tatacttttg aatatggatc
    15481 ttgtgtcaat tcacgttcga aatcaaattc ttgattatca aatctgttaa agaatgtttc
    15541 gtattcttcg actgataatt gctctctaga ttctagcata tttaagtgtt tctctttatc
    15601 taatgctttg tcatatcctt taacgattga accactaaag atttctccta ctgctcctga
    15661 accataacta aatagacata ctttctcttc tggttggaat gtgtggttct gtaataacga
    15721 aattaaactt aagtataatg atcctgtata aatgttacca acatctctat tccataatac
    15781 ggttctgttg caaagttg
//
LOCUS       DAKTGS010000060        15396 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.060, whole genome shotgun sequence.
ACCESSION   DAKTGS010000060 DAKTGS010000000
VERSION     DAKTGS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.060"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(433..531)
                     /locus_tag="O0286_001519"
     CDS             complement(433..531)
                     /locus_tag="O0286_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169901.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(736..1332)
                     /gene="recR"
                     /locus_tag="O0286_001520"
     CDS             complement(736..1332)
                     /gene="recR"
                     /locus_tag="O0286_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCY0169902.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1339..1656)
                     /locus_tag="O0286_001521"
     CDS             complement(1339..1656)
                     /locus_tag="O0286_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCY0169903.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1746..3443)
                     /gene="dnaX"
                     /locus_tag="O0286_001522"
     CDS             complement(1746..3443)
                     /gene="dnaX"
                     /locus_tag="O0286_001522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCY0169904.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3512..4036)
                     /locus_tag="O0286_001523"
     CDS             complement(3512..4036)
                     /locus_tag="O0286_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0169905.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4174..4275)
                     /locus_tag="O0286_001524"
     CDS             complement(4174..4275)
                     /locus_tag="O0286_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169906.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4344..4613)
                     /gene="ffs"
                     /locus_tag="O0286_001525"
     ncRNA           complement(4344..4613)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O0286_001525"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4675..5403)
                     /gene="treR"
                     /locus_tag="O0286_001526"
     CDS             complement(4675..5403)
                     /gene="treR"
                     /locus_tag="O0286_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCY0169907.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5428..7068)
                     /gene="treC"
                     /locus_tag="O0286_001527"
     CDS             complement(5428..7068)
                     /gene="treC"
                     /locus_tag="O0286_001527"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCY0169908.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7132..8559)
                     /gene="treP"
                     /locus_tag="O0286_001528"
     CDS             complement(7132..8559)
                     /gene="treP"
                     /locus_tag="O0286_001528"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCY0169909.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9030..9122)
                     /locus_tag="O0286_001529"
     tRNA            complement(9030..9122)
                     /locus_tag="O0286_001529"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9084..9086),aa:Ser,seq:tga)
     gene            complement(9391..10854)
                     /locus_tag="O0286_001530"
     CDS             complement(9391..10854)
                     /locus_tag="O0286_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCY0169910.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10872..15371)
                     /gene="gltB"
                     /locus_tag="O0286_001531"
     CDS             complement(10872..15371)
                     /gene="gltB"
                     /locus_tag="O0286_001531"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCY0169911.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
BASE COUNT         4701 a         3430 c         2152 g         5113 t
ORIGIN      
        1 acaagaatta attatacatt ttattaacat ttaagtcaat aactttttaa aattaatttt
       61 cgttagtaat tcaagttatt tcttaagttg agttgttttg tttgcctcaa caaataaata
      121 ctatacaggc tttcaaactg cttaacaaca gtaaaaatta cacctttgtc atctgtactg
      181 aacgccctta tcctaaaaat cgaacttaaa aaatccccac ttataaatag tacgtttttt
      241 agaacaagaa acagattttt ctctaaccat atcaatcaaa aaaaacgcaa aacagaacgt
      301 tattttttta ccagaaatta aaaataaatt tacaagtttt aaataatctc tcaaaacacc
      361 gctatacagg ctgatttaat aagctttaga ccaaagaagt acaacttact aagccagcat
      421 ctatcatttc aattacaaaa gaatttcatt tgcatagtca ttaaccgcat ttatcaaatt
      481 aatagcacca acacaccata accatagatc taattcgata atccaattca catcattact
      541 attagaagta acaaatcaca accaacggct tgcttcctaa agattaccca tccctcagta
      601 aaactaggta aaccatacca ccgccacacc gcaaataaag tagtacatca caccacacca
      661 taaaaaaagc cacaatgatt acactgtgac ttttcttact atagtattaa aatcaaaaat
      721 gtttaataga agacattaca tttctgttct acctgcgatt gctttagata atgttacttc
      781 gtcagcatac tctaaatcgc cacctaccga taacccttgt gctaatctcg tcactttgat
      841 acctataggc ttaactaatc tagaaatata catggctgta gattccccct ctaagttcgg
      901 gttcatagct aagattaatt cgctaacttc atcgtttttc aagcgttcaa tcaatgaagg
      961 aatattaata tcttctggtc caatgccatc cataggcgaa atagacccat gtaaaacgtg
     1021 atataaacct ttgtattctc tcattttttc catagctatg acatctttgt catcttccac
     1081 aacacaaata actgaacgat ctctttgctt atcttcacaa atataacatg gatcattttc
     1141 agtaatgtga ccacatacgc tacaatatgt taattctctc ttaacatcta ctaatgcttt
     1201 ggcaaactga acaacatcgt cttctttcat atctaaggta tgaaaagcca gacgttgggc
     1261 tgtctttgga ccaatgcctg gcaatttcat aaagctatca ataagttttg atataggttc
     1321 tggataatgc atctatgatc acattccagg gatgtttaag ccttgagtat gtttacctaa
     1381 acgttcttga gtaagctcat cagctttatt catcgcttca ttagtagctg ctaacactaa
     1441 gtcttgtagc atttcaatat cgtctgggtc tacagcttct tctttgattt caacgtcgac
     1501 aacttcttta tgaccagtta cagtaactgc aaccatgcca ccgccagctg ttcctacaat
     1561 acgctcttct ttaagttttt cttgttcttg agccattttc ttttgcattt tttgcatttg
     1621 tttcatcatt tgttgcatgt ttccgccacc gcgcatattc catttcctcc ttgaaaatca
     1681 ataaatattt atcaataaaa tgatgtttct ttcattatac atacgattat atcgcttgtc
     1741 atgtatcact cttcatctat cacatgtaca gtttcttcac cgaaaagatc ttttgctttt
     1801 tgagcaatat ctgtttgttg tgcttgttgc tttggcatat catcgccttc gtttttacga
     1861 ttttgtaaat attccgttcg aactctttgc cattgatctg atggtacacc aacaacttta
     1921 acgtttttat taacgatatt acatacaaca ctttctatac tactacgttt ctcgtcgtct
     1981 ttattgacga tttcacaatg gatctcttcc tcaaatttca caagtacgtg atcttcactt
     2041 gccgccacag gttccgaatt ttgcaataaa ctaacgagtg attttttatc attatttttg
     2101 gcatgatcaa tcacttcttg ccaatgatct ttcaacaatt tgatatctgc cttattcgct
     2161 ttatctagca cttttgcaat ttgttgcatt gaaaatgcat ttttagattt ttgtatgcct
     2221 ctcgcaggct ttttcgaaga tttttgaaca ggagcgacac tcactccttg tgcttttagt
     2281 gtttttagtt cttgctctaa ctgttccata cgttgcaaca atacatctgt gtttggcgat
     2341 gaagcaattt gtgctggttc agctacattc gcaatcactt gtggttgacc cttaatctgc
     2401 tcagctaatt ttactaacaa cacttcaaaa tgaacgtttt gattcacact aaaacgaatc
     2461 gacactaatg tatcattaat aagatcaatc atttgatata acatatctaa ttctaagttc
     2521 atcagtgctc gatactcagt atctttctca gatgttttat tcataatcgt atctctgaca
     2581 aaataaatca tatcatttat taggcgattc acttctttac cttctgttat aaactgatgg
     2641 tattttttaa aagatgcttg tacgtcacct tgtacaatat catcaaacaa gtgatccaac
     2701 gcttcatcat gtacgctacc tgtgacattc aacgcatctt gcaatgttaa cgtaccatca
     2761 ccaaatgcaa tagcctgatc cataatactt aatgcatcac gcataccccc ttcagacgct
     2821 ttagcgataa atgccaaggc ttcatcttca cattcaattt gttgtgcatc tgctacaaat
     2881 tttaaacgtt caacaatttg atctaggcta attgctttaa aatcaaaacg ttgtgcccta
     2941 gaaatgattg ttggagggat tttatgtggt tctgtcgttg ccaatataaa aatagcgtgt
     3001 gctggaggtt cttctaacgt ctttaaaagg gcattaaaag cacctgttgt tagcatgtgc
     3061 acctcatcta taatataaac tttatatttc gattcacttg gtgcatattt aactttgtct
     3121 ctaatatttc ttatttcatc aaccccatta ttactagcag catcaatttc tatcacatct
     3181 gaattagtcc cctgcgtaat gcctttacaa atatgacatt cattacaagg ttctccatca
     3241 gtgctattta gacagttgat tgctttagca aacactttgg caatactcgt tttccccgta
     3301 cctctcggac cactaaaaat ataagcatgc gactgttttt ctttcgaaat cgcattgcgc
     3361 aatgtcttcg tgacatgttc ttgtccgacg acatcctcga aactttgggg tctgtacata
     3421 cgatataagg cttgataatt caagttagca cctccataaa caattacctc tcattatagc
     3481 atgataatac ctttacttct taattgaaca attaataaaa atgttctgga aattttacga
     3541 ttccgtgtgg tgcatccgtc aaattatccc ataaatattt tacaagtaac ggtgcatcac
     3601 cagattctaa tttaatgtca tgctcagcag cattttgata acccaacttt tcaaagtagt
     3661 caaaacaatg gtctacaaca accgtactat actcttgtgc tttggcacgc tcttctactg
     3721 cttgaaccaa gccacgacct aatttttgtc cacgtaattc aggatgaact gataaagagg
     3781 caatcgccaa accataatac gtcttatcat cactattaat ttctacttca attaataaaa
     3841 cgtgtccaac gacatcgtta ttttcatttt tcgctattac ttctaattca aaattatagc
     3901 aaggagattt tcttaaatgt tttactttcg cacgtgcttg ccaactcgtt tcaggattat
     3961 catcaaaact ttcttcaata ctatttaaag atttatcata atctaactct gttaaagtac
     4021 ttaaatatat ttgcatatgt cctccgtagg catttgattg tcaataatca taacgtatct
     4081 cattaataat tctattgtaa gatactcccc ctcatttcac catcatttct ttatcatcaa
     4141 attataactt cttacttttc attgacacaa aaatcattca aactgcacat cgagttcact
     4201 tgaatcaaac ttcacatata aaaaagctac ttcccacaaa catgttccac gtataatacg
     4261 ctgaattgtc ttcaagaaag tagcttctat aattatatat tttcaactct taataatcgg
     4321 tttaatattt aaaaaataaa aaaccgtgca cctaagcatc gaacgtaatt cacgaacgta
     4381 accaaagtcg ttagctaaat ctcggctacc ctacggcaca tatgatgatc cacttaatgc
     4441 tgcttccgtc aggacctgac atggttcatg ggttcatatt gcataggacc gaaatcttca
     4501 aacactacgt gctttgggca gacttcgcaa aaatacggcc tcaacaaagg aattaagcct
     4561 cgcataaagc ggatttcgag tacagggaac cgctacctcc ccacctagca cggcaagata
     4621 tatattacta tattttaata gttaattgca agtataaatc atttatatca ttgtttactt
     4681 tatacgacgt cttgagaagt cattaaattt aaattcattt gcaagatgtt ttgaaatatt
     4741 atattgaaac ggcattgtat tttctaaata cacaatactt cgaactgttg ctgaataagc
     4801 caccgataca tcaccaaaca attgatatgc ttgttcatca aacggttcaa aagtaataga
     4861 cttacttgaa taactaatat taagatttaa taccttttcc aaataatcat aaatagaatc
     4921 actcgcaaca tcattaccta tatctgaaac aatcgacttt agaaaataat cttcatcaac
     4981 aatcttcaca tgttggttaa gccgacgagt tctaacaata tgaatgagtg attcactaga
     5041 attgatgttt aaagcatgtt gaacttctgg aacttcatgc gcttcaacaa cctcattcac
     5101 aacaacttca gttaaatatg cgacgcccat ttcttcttgc atttctttaa aactaacaag
     5161 ttcagaaaat ggaaactctg taacctcctg ataaatgaca agtgaccctt taccatgaat
     5221 cttttgaatc atgccgtcta atgccaacaa atctaatgcc ttacgcacgg tctctcgaga
     5281 tgactggtac aattgcacca aatcatgttc agacggaatt tgttcaccat attgaatctg
     5341 cccgtttaat atatcttctt tcaacgcctc ataaatcttc ataaactttt tttgtttcgc
     5401 catataaacg catccactca atttatttta cgcgtttgtt tcgattacaa ttgcaccaaa
     5461 cccgcttatc gtattatttt ccactgtgcc tgtttgaatc acaacacaac cttctctagc
     5521 caatccttct ggcaaatcaa cagccgatgc tgagaaattc gcaattacta gccattgttg
     5581 attcttataa tgacgttcat aaacaaataa atgatcatga tccataaaac gtggcacaat
     5641 gtctccatac gtaataatat catgcgtatg tcttaattgt attaattttt gatacgtata
     5701 gaaaatagac tctttattct gaagtgcttg tctaacgttg acctgatgat aattttccga
     5761 aatatcaatc caaggcttac cagctgtaaa tccagcatta acatcatcac tccattgcat
     5821 aggcgttctc gaattgtctc gtgacttctg tcctaaaatc gctaacacat ccgcttcagc
     5881 atgcccttca cttaacaact gatggtaggc attaatcgat tcaacatcac gatattgtgc
     5941 tattgatgta aaatgtgggt ccgtcatacc aatttcttca ccttggtaaa tatatggcgt
     6001 cccttgcaac atatgcagtg cgatagctaa cattttagca ctttgtatcc tcatctcttc
     6061 cgacgtatca tcaccaaatc tagacactac ccgtggctga tcatgattac accagaaaat
     6121 cgcgttccat ccgccaccgt cataaatacc tcgttgccat tgcatcagaa tttcctttaa
     6181 cttatgaaaa tcaagcttcg catttgtcca cttttcacca tcaacataat caacctttag
     6241 atgatgaaaa ttaaaaacac tattcaattc ttggcgttct ggttgtgtat acttaataca
     6301 attttcaatc gtcgtcgaag acatttctcc tatagtcatc atgtcagtgt taccaaacgt
     6361 ttgacgattt aattcatgca gaaactcatg cactctagga ccatccgtat aaaattcttt
     6421 acctattttg tcagagtcct taaattcacc tttagaaatt aagttaatga catcaaatcg
     6481 aaaaccatca acgccgaagt ctatccaatg attgactatg cgatataacg attgacgtac
     6541 ttccggatta tcccaattta aatcagcttg actgacatca aataaatgta aataatattc
     6601 atctgtctca gaatcatact tccatgcatt accaccgaat ttagaatacc aatttgttgg
     6661 cggcccgtct tcagatgatc tgaaaaagta ataatctcta taagggttat ctttagattt
     6721 acgggcttct ttaaaccatt catgctccgt cgacgtatga ttaatgacaa tatctaacat
     6781 cactttcaag tctttttgat gcgcaacttt gattaacttt tcaaaatcat ccatcgttcc
     6841 aaagtcttca ttgatttcta aataattgct gatatcatag ccattatcat tcatcggtga
     6901 ttcatacact ggtgttaacc aaatataatc aacacccaat aacttgatat aatccaattt
     6961 ttctataatt ccattgatat ctcctatacc attccccgtc gtatcattaa acgacttagg
     7021 ataaatttga tatacaacgg attttctcca atctatttct ttcgacacac aattaccacc
     7081 ttataacgta ataaaggacg tccaaataac gaacgcccct tttttatttt attaatcttc
     7141 aacaatttct ttcgcttttt gtttactaaa atgagacatc acaattgtta gtatacatgg
     7201 tacaacaata gcaatagctg tcacaataag atatactggc caaaattctt tttgaattga
     7261 aatgaatgct ggcacgccac caacaccaac ttttccaagt acgttattca taccaacgat
     7321 tgcccccaat acacaagacg ttgatatcgc agcgataaat ggatatttca gaggtaagtt
     7381 cacaccgaac atggctggtt cagtaacacc taacatacca gaaatacaag atgttaatgc
     7441 caagccttct tctttaacca ttttacgacg tttatagaca aaccatgctc caaatgctgc
     7501 agagccctga caaatattgg aaatcgcaac aattggccat aaatacgtac cgcctaagct
     7561 gctacccatc aattggaaat ctactgctaa aaacatatgg tgtagtcctg taattacaag
     7621 tggtgcatat aacaatccat atattgctcc gccaagccat cctgcatgtt ggaatataaa
     7681 tgtaacacca gatgtaatac ctgtaccaat caataacgca actggtccaa tgataataaa
     7741 tgctaaaaat ccagtaacta aaagcgctac gggtccaacg accaacattt ttatcgaatc
     7801 gtgaacgact ttatttaatc ctttttcaat tttagctaga acgtaagctg caattaaaac
     7861 tggcaacact tgaccttggt aattcaactg cttaatctct aagccaaata agttccacgt
     7921 cggaatattc ccttttgcca aatcatactg agatactaat tgcggatgca ttaaaatcaa
     7981 acctaagact atgcctagaa tcggactacc accaaataca cgcatactac tccaaccaat
     8041 taatgctggt aagaaaataa atgccgtact cgcaatcaca ttaatgatgt ttgaaatatc
     8101 agcaatttgt ggatacatct caataagtgc ttttggacca aataaacctt tcattgtaag
     8161 taaattattg attcccatta acaaaccagc tgtcacaatc gcaggtaata ttggtataaa
     8221 aatatccccc aacaatttga tcaaacgttg tactggattc cctttttgtg cagctgcttg
     8281 tttcgcttca tctttcgaag cttcttgagc acctgtttca tcaataaact gcttatacac
     8341 ttcatcgact gttcctggac caatgacaat ttgatattga tggtctgctt taaactgccc
     8401 cttgaccaac gcgttattac ttaatgcgtc tttatcaact ttactttcat ccttcagcac
     8461 taaacgtaat cgtgttacac aatgcgttgc agcttcaaga ttttcttttc ccccaatagc
     8521 ggttacgatg gcttttacat cttctctttt tacagccata tatttcactc ccttaatgtc
     8581 ttaaaacgag tataacttgt ctagacaagt tatgtaaagg ctttcaacaa aatattttta
     8641 ttttgtcaca attatctggg gtagccattt cagaaaatca atgagccata acgacatatg
     8701 tagcaataac tcctcgctac aattgcgtta ttaacaatac ctcaccacgc tctcaccatt
     8761 ataaagtgta gatgcatggc caactcctaa attgaaaaat gcatatctat aaatagcaaa
     8821 cttgtccaca caagccatcc atcatatttc acaaaacaaa aaagacattt agctatgtat
     8881 attgcacaag ccaaatgccc ttccaatctc aatgccttat catttctatt atcaaaaatt
     8941 acgagccatt caaagacaaa taaaaaaggt aaatactcaa aatttgagca cttaccttca
     9001 ttatatatag aatttaatat aaatattgat ggcggaggaa gagggattcg aacccccgcg
     9061 agccgttaag cccctgtcgg ttttcaagac cgatcccttc agccggactt gggtattcct
     9121 cctaaacaca atatttatca tatatgaacg ttctgtaaaa gtcaatattg tttttaagca
     9181 ttacatgatt accacaaatc attgtcttcc actacagttg acctataaac gaatgtaaaa
     9241 aatcactact tataattaat ctatttgttg tcttacaaac ctttgttttt aggttacaaa
     9301 gatggcatgt gacgttgata gatactagat tttttcaaac tcgattcagc atgtcacaac
     9361 gtcaacgtaa ccaccatttc catacaaaga ttatacacaa actttactag ctaaatactg
     9421 atctactgct ttcgctacgc ctctaccttc tttaattgcc catacaacta aactttgacc
     9481 acgtctagca tctccagcag caaatacctt ttcattatta gtttgatagt ttgtatcatc
     9541 cgccacgatt cgatttctat ccgttttaat gttaaaagca ttcggtactg ttggttctgt
     9601 accttcgaag ccgattgata ataatacaag gtcagcaggc caaaatcttt caggtccttc
     9661 tttcatgacc ataccatttt cgccttgctc taaaatttga gtatacaaac cacgtatgtg
     9721 tcctttatcg tcaacatcgt aacgcattgt ttgaacacca tatgcacgtg gttccttacc
     9781 aaacttagct tcgtactctt ggtgcgcata gtccatttta aacaccggca ttgctaaagg
     9841 ccatgatgca ttttctgtaa atgtaattgc ttctggcaat ttcgtatatt tattaaattg
     9901 aacaatcgat ttacaatttt ctcttaatgc tgtcgctaca cagtctgccc ctgtatcacc
     9961 agcaccaatg ataatgacat tcttatcttt tgcagttatt gttatatcat caatttctcc
    10021 atttaacaac tgcgtttgtt cagttaaata atccatagcg aaatgtatac catcacccat
    10081 gcgtccttct aaaggtaaat ctctaccttt ttgtgcacca gtacataata taatggcatc
    10141 atactcagat tctaacgttg ctttatcaat atcaacgccg acttcaacac cattaatgaa
    10201 agtaatgccc gcttcttcca ttaacttaat acgacgtcga accacatctt tatcaagttt
    10261 catattcgga ataccacaca ttaataaacc gcctgattct ctagcacgtt cataaatagt
    10321 tacttgatat cctagtagat taagttcttc agcagcagct aatcctgctg gaccgcttcc
    10381 aacgattgcc actttttcat ctctacggcg actcggaact ttcggcgcta cccaaccatt
    10441 ttcaaaagct tcatcaataa ttgtgcgttc aataccttta atcgcaatcg attctctatt
    10501 aatcttcatc acacaagcac tttcgcatgg tgcaggacat acacgccctg taaagtcagg
    10561 aaagttattt gtttcgctta agcgttcata agcagtttta aaatcttgat gatacactaa
    10621 gtcgttccat tcaggaatgt agtttccaat tggacaacct attgtttccc taccatactg
    10681 ttgtccggtt tgacaaaacg gcgttccaca atccatacat cgtgcacctt ggatagaggc
    10741 atcttcttta gtaaatcgtt gttgatatgc tttatgatgc ttcaaacggt ctaccagtga
    10801 taattcacct aagtactgtt tgtcatactt cataaatcct ttaaattcac ccatcgtatc
    10861 tccccctttc cttaatacac aacggctggt ttatgtttag catcgattgt tttactgtca
    10921 tcgtaaaatg cagctaacat cgcttcatct tcattgtcat gtaatgattt gtgcaaatga
    10981 attttttgca tcattaattg ataatcttta ggaataactt taacgacgac atcttcaatg
    11041 cgatcaaaat gttttaacac atgaatcgct ctcgtactat tcgtgtgtga cacatgttct
    11101 tccagcattt gcttaatgaa tgctttttct tcttggtgtt taatctttgt aaacgaaagc
    11161 gtatctagtt gattattttc aacaaaagct tctacatcag acgggataac gtaagcaata
    11221 ccaccactca taccttgacc gaagttctta cctacatcac ctaaattaat gacatgtcca
    11281 ccagtcatat actctaatcc atggtcgccg ataccttcaa cgacaacatc tacaccacta
    11341 tttctaatac agaatctttc tcctgcacta ccgttaataa atgccttacc acttgtcgca
    11401 ccatagaatg agacgttacc agcaataatt tcattttgtc gttcttcaaa aggtgctttg
    11461 acaatgaccg taccaccaga taatccttta ccaacatagt cattcgcatc tccagtatga
    11521 tgaatcatta agcctttcgg tgcatatgct gcaagacttt gaccagcatg accattcgta
    11581 taaacattaa ttgtattttc aggaagtcct gcttctccat attgtttcga aatctcacta
    11641 cctgtaataa cccctacatc acgttgttca ttatttactg taaagctacc tgtatagcga
    11701 cgcccttcag caatatatgg cttcgttact tcatataaat ttgttaaatc aaatccatgc
    11761 tcaagattat gattttgttg aatttctttt gtgtttggcc catcgaaagg acataacagt
    11821 ttttcaacat caatactagc cgctttgcta ttcgctttta atgttgatga tcgttgtaat
    11881 aaatcagttc ttccaactaa gtcttctaca cgtttcaaac ctaaagatgc taaaatttct
    11941 cttaattctt gtgcaataaa atgcataaaa ttaacaacat gatgtgcttt acctctatat
    12001 aaagcacgta aatctttgtt ttgagttgca actcctactg gacatgtatc tttatggcat
    12061 acacgcatca taatacagcc caacaccact aatggtgcag ttgcaaatcc aaattcttcc
    12121 gctccaagcg cacatgcgta cgctacatct ttaccagtta ataacttacc gtctgtttct
    12181 aacttaacac gacttcttaa gtcatttagt tttaatgttt gatgtgtttc tgctaaacca
    12241 atctcccaag gaacaccggc atgctgaata ctcgttttgg gtgaagcccc tgtaccacca
    12301 tcgtaaccac tgatgacaat tttatctgca aatgcttttg ccaccccaga tgcaatggta
    12361 ccaacacctg ttttcgaaac taattttacc gcgatatctg catctttatt cgcatttttc
    12421 aaatcatgta tcagttgcgc taaatcttct attgaataaa tatcatgatg tggcggtggt
    12481 gaaatcagac cgatacctgg cgttgaccct cttgtcttcg caatccacgg atatacctta
    12541 gtaccaggta attgaccacc ttcaccaggc tttgcacctt gcgcaacttt aatttgaatt
    12601 tctttggcat gttgtaaata atcactagtt acaccaaaac gcccagaagc aacttgttta
    12661 atcgcactta ctttgttgct tccatcaact tgtacttcat aacgttttgc atcttcgcca
    12721 ccttcaccac tattactctt tccacctaat tggttcatgg cttgtgctaa cgtttcatgt
    12781 gcttccgctg aaatcgatcc ataactcatc gcccctgtat taaagcgttt gacaatgtca
    12841 cttaccggtt caacttggtc gatgtcaatc ggtgtacatg ctttaaattc aagtaaatgt
    12901 ctaatgtgat ctgttctatt tttgttcacc gcttcagagt atgctttaaa ttgcgcatag
    12961 tcattttctt tacatgcgtg ctgcaataag aaaatagatt ccggattaaa agcatgatgt
    13021 tgaccttgtt gtctccattg gaatgtacta cctgatgcaa gataattatc atcactttgt
    13081 tgacgtgctt tattttcagc atcaatttga tcaatcgaaa taccagataa cttagactgt
    13141 gtcccagtaa aataacgatc aatcacatca tgagacaagc caatcgcttc aaatatttgt
    13201 gccccttgat aactttgcac tgtcgaaatt cccatcttag ccattacttt aatgacacct
    13261 tctgacaata catccgtata tgtcttaaca ttatcgacaa cggtgccttg taacccttct
    13321 gtcaatgtca gttgttcaac tgtacgttgc gctaggtatg gcacaattgc attcgcgcca
    13381 tatgcgagta aacaagcaac atgatgcact tctcgtgtct caccagattt agcgactaaa
    13441 cttgtagaca tacgtaaatc tgctttaata agtaattgat gcacatgact tattgcgagt
    13501 aacatcggca ttgcaaagcc attgctatca actaatccac tatcatctaa cactagaatt
    13561 tgagcgcctt gctttacagc attcactgct tctcggccta atgcttctaa cgcatcttcc
    13621 aaatcccctt catataccgt tgataaataa gttaatttaa aatgttcctg atcaatcgct
    13681 gctaagtgtg attcattcaa taccggcctt ttcaattgaa tacgatctaa aaccgtttcg
    13741 tcaggtgcta gtaagttacc ttcgccacct aaataagaaa gttcactcgt tacgattttt
    13801 tcacgatacg catcaattgg tggattcgta acttgtgcaa acagctgttt aaagtaatta
    13861 aatagtgatt ctggtcgctc gttcaacact gcaattggcg catcatatcc cattgcaccg
    13921 ataggatcct tcttaccttc tacaagttcc tgaatatact tatgaatctc ttctttcgtg
    13981 tatgcaaact gacgttgtaa tttaaataac gtctcatctt tccattgcga atcttgatat
    14041 tgtatatttt caaaatcaaa gtcaacttta tggttatcaa tccacgcttt atatggtaat
    14101 tctccagcaa tcgcaccttt taaatcatta ttttcaatga ctttattctg tttaaaatca
    14161 acaagcaata actttccagg attcaattga cctttaaaag caacattact ttcaggtacg
    14221 tccacaacac ccacttcaga tgaaaagaca ataaagttat ctttagtaat cgtataacga
    14281 cctggacgta atccatttct atctgtaagc gcgccaagtt tgtcaccgtt acagaacgaa
    14341 atcattgtag gaccatccca cggttccatt aaataactat aaaattcata aaacgcacgt
    14401 acatttgcat cattcgcttc attatataac caaggttcag gtatgagtaa catcgctgcc
    14461 ttttctggct ccatggctaa cgataagaac tctagcgcat tatctacaat ggcagagtca
    14521 ctaccatcct catcgacaat ttgaaacact ttatgttgat cctcgccaaa taatgtttcg
    14581 attaatttat gttggcgtgc tcgcatccag tttacattac ctttaatcgt gttaatctca
    14641 ccattatgca ttaacatacg gttaggatgt gcccttttcc aactcgggaa tgtattcgta
    14701 ctaaatctcg aatgcactaa ccctagcttt gattgatata aatcatccga taaatctgta
    14761 tatagttttt taatttggtc tgatcgtaac caacctttat atacaattgt tttgcgtgat
    14821 aagctcgtaa aatacaattc taaatcgcac tgagtcgaat agaactctaa ttgttttctc
    14881 gctaaaaaca aacgcttttc aacatcttca atgtccctaa tatcaataaa cacttgttga
    14941 atgactggca tcgtatctgc tacatgttta gcaatggcat ctttattaac tggtacatta
    15001 cgataaccaa gaattgataa cccttcgcct tcaaaatatt ttttaaaaac tacttcatgt
    15061 tcagaaccta aaatgcgttc tttggaaaaa aataacccca cggcatattc accttcacct
    15121 gggatatcaa agtccgttac atgttgtttg aaaaatgcaa aaggtatttc agtcataata
    15181 cctgcgccat caccagtgat gccatctgcg ccgaccccgc ccctgtgatc taagcgtcgc
    15241 aacatttcaa gcgatttatc aatgatgtcg tgagaccttt tattatccat attcgcataa
    15301 aaaccaatac cacacgcatc atgttcctca cgatagtcat ataagccttt aattaatttc
    15361 tcattgtgca tgatgcacac ccctttttta gaatat
//
LOCUS       DAKTGS010000061        15310 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.061, whole genome shotgun sequence.
ACCESSION   DAKTGS010000061 DAKTGS010000000
VERSION     DAKTGS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.061"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            21..299
                     /locus_tag="O0286_001532"
     CDS             21..299
                     /locus_tag="O0286_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCY0169912.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            314..682
                     /locus_tag="O0286_001533"
     CDS             314..682
                     /locus_tag="O0286_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169913.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            670..966
                     /locus_tag="O0286_001534"
     CDS             670..966
                     /locus_tag="O0286_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCY0169914.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            1300..2406
                     /locus_tag="O0286_001535"
     CDS             1300..2406
                     /locus_tag="O0286_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCY0169915.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            2400..3740
                     /locus_tag="O0286_001536"
     CDS             2400..3740
                     /locus_tag="O0286_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCY0169916.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            3838..4728
                     /locus_tag="O0286_001537"
     CDS             3838..4728
                     /locus_tag="O0286_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0169917.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(4875..5705)
                     /locus_tag="O0286_001538"
     CDS             complement(4875..5705)
                     /locus_tag="O0286_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0169918.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(5725..6735)
                     /locus_tag="O0286_001539"
     CDS             complement(5725..6735)
                     /locus_tag="O0286_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0169919.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(6833..7537)
                     /locus_tag="O0286_001540"
     CDS             complement(6833..7537)
                     /locus_tag="O0286_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0169920.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(7530..8087)
                     /locus_tag="O0286_001541"
     CDS             complement(7530..8087)
                     /locus_tag="O0286_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0169921.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            8218..8517
                     /locus_tag="O0286_001542"
     CDS             8218..8517
                     /locus_tag="O0286_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCY0169922.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            8537..9358
                     /locus_tag="O0286_001543"
     CDS             8537..9358
                     /locus_tag="O0286_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCY0169923.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            9375..9752
                     /locus_tag="O0286_001544"
     CDS             9375..9752
                     /locus_tag="O0286_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0169924.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(9902..10132)
                     /locus_tag="O0286_001545"
     CDS             complement(9902..10132)
                     /locus_tag="O0286_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0169925.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(10352..10852)
                     /locus_tag="O0286_001546"
     CDS             complement(10352..10852)
                     /locus_tag="O0286_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169926.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(11050..11619)
                     /locus_tag="O0286_001547"
     CDS             complement(11050..11619)
                     /locus_tag="O0286_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0169927.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            11774..11902
                     /locus_tag="O0286_001548"
     CDS             11774..11902
                     /locus_tag="O0286_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169928.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            12029..13084
                     /locus_tag="O0286_001549"
     CDS             12029..13084
                     /locus_tag="O0286_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0169929.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            13692..14393
                     /gene="isaA"
                     /locus_tag="O0286_001550"
     CDS             13692..14393
                     /gene="isaA"
                     /locus_tag="O0286_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCY0169930.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            14669..15157
                     /locus_tag="O0286_001551"
     CDS             14669..15157
                     /locus_tag="O0286_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0169931.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
BASE COUNT         5142 a         2369 c         2789 g         5010 t
ORIGIN      
        1 ttaaatggag agaggtgtaa atgatgagta cagttcaaag tgatattttt aagaccaata
       61 gtgcatcatc atctattaaa agcgctgttg aaacatgtaa taatgtgtcg aaaccggata
      121 aagatgaaag tacaacagta agtggaaata ataatgctca tagtgtgata gatgatttga
      181 tgagtaagaa tcaatctgtt gctgaagcaa tacgaactgc gagcgataat atacaaaaag
      241 ttggtgaggc ttttgaccaa actgacgtaa tgattggtaa tgaaattggt aaaaattaaa
      301 acgtggtgaa atgatgtcga ataaactgga tgaaatcaat aaaataatca cagcgaaaca
      361 tgagcaaatg gatgacttat atgatgaaaa gcgagaggtt aaagcattga tagatgaaag
      421 tgatgcgctt aatcattcga tagatcaatt atatcaacat ttaggtgagc gttattatag
      481 tagcaatatg gctagtcgta tggaacagtt ccgcgatgaa tttcattttg cgaaacgacg
      541 ttcaacggaa gcgttatacg agcagcaaca gcaaattcaa catggcattc gtaaagtgga
      601 agaagagatg attgacttgg aaatgcgaag gaatgttgaa attgagacgg tgacaaagga
      661 ggaaaataaa tggaaacaat aggaagcatt atttatttaa aagaaggttc gcaaaagtta
      721 atgattatta atagaggacc aattgtagaa attgaaaatc aaaagtatat gtttgactat
      781 tctgcatgta aatatccgat tggtgttgta gaagatgaaa tttattattt taacgaggaa
      841 aatatagatt cagttatttt taaaggttat tctgatcaag atgaggttag atttcaagag
      901 ttgtttgaaa atatgaaaca aaatttggat agtgaaatac aacgtggaga agttacacaa
      961 caataaagaa atactttttc tttattgggg tgggacgacg aaataaattt tgtaaaaata
     1021 tgatttctgt ctcaccgcca aaatgttatc tccaagtact gaggctggat gataccatga
     1081 aatgtcattc ataatcattt gaactaaacg tagcagcctt aaattttaaa aaaagacaca
     1141 taccaacttc cgaaatgtag atgaattctc tacaataacg gaagtttttc ttttaatatt
     1201 gaaatttctc aaggataggt ctatacttta taaatcgtaa ttattacgat ttataatcaa
     1261 aaacaataac ttgaaataga tcattgaggg agtgttaata tgcaacatca taaagtggct
     1321 attatcggtg ccggtgctgc aggtataggt atggccatta ccttaaaaga tttcggtata
     1381 acagatgtca ttattttaga aaaaggaaca gtaggacatt catttaaaca ttggccgaaa
     1441 tcgacccgta cgatcacgcc atcatttacg tctaatggat ttggcatgcc tgatatgaat
     1501 gcaatttcca tggatacttc accagcattt acatttaatg aagaacatat ttccggagaa
     1561 acatatgctg aatatttaca agtggttgcc aaccattacg agctgaatat ctttgaaaat
     1621 acagttgtca caaatatatc tgtagatgat gcatattata cgattgcaac gacaacagag
     1681 atatatcacg cggattatat ctttgtcgca acaggtgatt ataatttccc taaaaagcca
     1741 tttaaatatg gtattcatta tagtgaaatt gaagactttg ataactttaa taaggggcaa
     1801 tatgtggtta tcggaggtaa tgaaagtggc tttgatgctg catatcaact tgcaaaaaat
     1861 ggctctgaca tcgcacttta tactagcaca accggtttaa atgatccgga tgctgatcct
     1921 agtgttagat tgtcacctta tacacgtcag cgactaggta atgtcattaa gcaaggtgct
     1981 cgcatcgaaa tgaatgtaca ttatacagtt aaagatattg attttaacaa tggacagtat
     2041 catatcagtt ttgatagcgg acaaagtgtg cttacacctc atgaaccaat actagcaact
     2101 ggctttgatg caacaaaaaa tccaatcgtt caacaattat ttgtgacaac aaatcaagat
     2161 attaaattaa caacacatga tgaatcgaca cgttatccga atatttttat gattggtgca
     2221 acagttgaaa atgataatgc caaattatgc tatatctata aatttagagc gcgatttgca
     2281 gtacttgcac atcttttaac acagcgggaa ggcttaccag ctaaacaaga tgtcattgaa
     2341 aattatcaaa aaaatcaaat gtatttagat gattattcat gttgtgaagt gtcatgcaca
     2401 tgttagaagt gaaatatgat atgagaactg ggcattatac gcccatacct aatgaacctc
     2461 attatttggt tattagtcat gcggataaac ttaccgcaac agaaaaagcg aaattaagat
     2521 tattaatcat aaaacagaaa ttagatattt cattggcaga aagtgtagtt tcttcgccta
     2581 tagcgagtga acatgtgata gaacaattga cactatttca acatgagcga cgacatttaa
     2641 gacctaaaat aagtgcgaca tttttagcct ggttgttgat atttttaatg tttgcattgc
     2701 caatcggtat cgcttatcaa ttttcagatt ggtttcaaaa tcagtatgtg tcagcatgga
     2761 tagaatattt aactcaaaca acattgctca atcacgatat attacagcat atattatttg
     2821 gtgattatgg tgtgctatca cttggaacat attcgctcgt atgggcattg ccggttgtaa
     2881 tattgattag tttatcaact gctataattg atcaaacagg actcaaatca tggatgatat
     2941 gggcaattga accgtcaatg ttatggatag gattacaagg taatgatatc gtgccactat
     3001 tagaagggtt tggatgtaat gcagcagcta tttcacaagc agcacaccaa tgccatacct
     3061 gcacgaagac acagtgtatg agtttaataa gctttggtag ttcttgtagt tatcaaatag
     3121 gtgcgacatt atctattttt agtgtagctg gaaagtcatg gctatttatg ccgtacttaa
     3181 tattagtact tttaggtggc atcttacata ataggatatg gttgaaaaag aatgatcaac
     3241 aacttagcgt tccgctacct tatgataggc aattacatat gccaaatata cgtcaaatgt
     3301 tgctacaaat gtggcaaaat atacaaatgt ttatcgttca agcgctacct atttttatca
     3361 caatctgtct tattgttagt attttatcac taacgccaat tttgaatgtt ttatcacaaa
     3421 tatttacacc tatattatcg ttattaggca tctcgtcaga attgtcacca gggattttat
     3481 tttcaatgat tcgaaaagac ggcatgctct tgtttaattt gcatcagggc gccttattac
     3541 aaggaatgac agcaacacag ttactactac ttgtgttttt tagttcaaca tttacagcgt
     3601 gctcggtcac aatgacgatg cttttgaaac atttaggtgg tcagtcagca ctaaaattaa
     3661 ttggaaagca aatggtgaca tcattgtctt tagttattgg tgtaggcatc attgttaaaa
     3721 tagtaatgct gattatttaa aaaaaatgaa ctataactga atatagagtc atgtcagtca
     3781 ataggagatc tatcttggaa tatgctattc atatgaagta taagaggaga gtcgcagatg
     3841 aaaatagtta ttataggtgg gtttttaggt ggcggtaaaa cgactgtctt aaatcatttg
     3901 ctcgctgaat cattaaagga atcgctgaaa ccagcagtca tcatgaatga atttgggaaa
     3961 atgagtgttg atggtgcctt agtatctgaa gacatacctt taagtgaact gacagagggg
     4021 tgtatctgtt gtgcaatgaa agcagatgta tcagaacagt tacatcaatt atatttaaaa
     4081 gagcaaccag acattgtatt tattgaatgt agtgggattg cagaaccggt ctctgtctta
     4141 gatgcttgtt taacgcctat tttagctccg tttacaacaa ttacacatat gattggtgta
     4201 atagacgcaa gcatgtataa acacattaaa tcattcccta aagacatcca aggcttattt
     4261 tatgagcaat tagcatattg ttctgtctta tttgttaata aaatagattc agcagatgtt
     4321 gaaacaacga gcaaactatt gaaagattta gaagttatta acccagaggc cgatatacaa
     4381 gtcggtatgc atggcagcgt cactttgcca atatcagtta gacaaatgac agcaacttct
     4441 gacaataaac ataagtcttt acatcaaatg attaatcatc aatttgtgca atcaccagtc
     4501 aaatgtacta aagcagagtt tataaaacgt ttagcatgcc ttccgtctca tatttatagg
     4561 ttgaaagggt ttatgacatt tgaagacacc gcacatacgt atctcattca atttacacaa
     4621 ggacaatatg aattaacacc tgtagcattt tcaaaaaaag tgccagaata tttggtgctt
     4681 atcggaaagg gtatttcaaa agaagactat caatgtttgg aacagtagtg ttttcagtgg
     4741 aagagaatgg ttaacatgcc ttcatgtata ataacgagtt gatttgaacg tttaagcgta
     4801 aataaaaata agcttggtca gccatcaaat ataatttgaa aactgtccaa gctgttttat
     4861 tagagaacaa tcaattaacc ccacatattt aataatacat cagcaaagcc ttcaggtttt
     4921 tgaatataac ctaagtgacc gcctggaata tctacaatag gtatgccagt ttctttattt
     4981 atataaaagt taacatcttg tgggaaggag cctctagaat ctgtcccatt tagtagggtg
     5041 attttatcgc tgtattttgt gaaatcatcc aaagtaatat ttgaatgcgt atattgtcta
     5101 atttcaaatt ctaaccagaa catcgtacgt ttgtactgtt ctatacgtcc ttcttcagta
     5161 tcagcaggtt gagacatcat ttttgcatca attggtgcga tatttaatgt ttcgccaaat
     5221 gttttcatgc ctttttctaa gccttctgtt aaaatttgat gcacaatgtc atcattttta
     5281 tctttccaat aagtactgtc tggtaaaaat gtattaattg gtggttcgtg aaatgcaatc
     5341 tttttaacga cttcagggta atcttttaac acatgcatcg caacgattga acctgaactt
     5401 gaacctaata tatagacagg ttcatcactt aatgactttg caagttcggc aatgtcctgt
     5461 gcgtcgcgtt tgacacgata atcactgtca gggtttgaag cggaatcagg gagtggttca
     5521 gttaactcgc tttctccata atcacgacga tcaacggcta caacagtaaa atggtctttt
     5581 aactgttctg caagaggcag aaaaatgtct ccggtaccgt ttgcaccagg aataaagatg
     5641 agcacgggtc cttgtccgac ttggtggtat cgtaatttag cgccttgtaa ttctaaagtt
     5701 tccatattca atgacctcca tttgttaatt gttaggtgat aaacctaata atttagcacc
     5761 atttgtataa cttattttct ctttttcttc atctgttaaa cccagttcat ctaaaaatac
     5821 acctaatttt tcaggctcaa tatatggata atcagcagca taaagaattc tatcaatacc
     5881 tacttctttc ttgactaaat caaactgtgg cttcgttaac atgccactcg gtgtgatata
     5941 aaaattattt ttaaagtaat agcttacagg gtggttcaaa tgttcagcga ataaagcttc
     6001 atccatacgt tctaagaaga atgggataaa ctcaccccaa tgtccaataa tcatatttaa
     6061 ctttggataa cgatcaaaaa taccagataa tactagatgt attgcatgaa tgccgacatc
     6121 aatgtgccaa ccataaccaa aacaagcaaa tgttgccgca gttacttcag gataatttcc
     6181 tttatagtat gattgataaa tgtcactgtt aactggcgcg ggatgtagat aaatcggtac
     6241 gtctaaattt tcagctgttt tgaaaataat gtcatatttg tcttgatcaa gaaaaccatc
     6301 ttgtgcacgt cccataatga gcgcaccttt gaatcctaaa tcattgatgc aacgttcgaa
     6361 ttctcgcgct gcggcttcag gctcattgat aggtaaagtt gcaaagccta caaagcgatt
     6421 gggatattgt gcaatatagt ttgccaattg atcatttgct ttttgacata attcaatggc
     6481 tttctgacca accaaatttg aaggagaacc atttccataa gataagactt gaatttgaac
     6541 gtcttgatta ttcataaatt ggatacgttc atcatgatgt gataattcgt cggcatttgt
     6601 aaaacctgtc tttttttcaa ggccttctaa cattactttc atcggtacac ctttaggatc
     6661 tgctgatatc gcattcatcg tttctttttg aatatcttca atgacataat gttcttcaaa
     6721 cgtaatactt ttcatttact tcgcctccat attgtattgc atgtttattg catctattgc
     6781 agaagcattt tttatatacc tctaatttca atgtttgtaa cataaaattg atctaccaag
     6841 gcatctctcc atcgccatta ataaatgtac ctgttgggcc atctgcacca atcgttgcta
     6901 attgaatgat tggcttgatt ccttcagaaa cgtgtttgga attattacta aaatcaccaa
     6961 ctaaatcagt atttgtagcg cctggatcag cagcattgat ttgcatgtta ggtaatcctt
     7021 tagcgtattg tagcgttagc attgttactg ccgatttaga cgaacaataa gctaatgaat
     7081 tcactttaga ttcagctgtt tcggggtttg taaccattcc aaatgaacct aaaccacttg
     7141 atacgttgac gacaacaggt tgttcagatt tttctaagag agggacgaat gtattcatca
     7201 ttcgtacgat accgaataca ttcgtttgat atacttcttc aacgtcacga ggtgtcaatt
     7261 tggaaggtgc tgaaaattga ccagatatac ctgcattgtt aatgaggata tcaagacggc
     7321 cttctttttc agcaatcatg ttataagcat ttttgactga gtagtcactt gtaacatcta
     7381 attgtacata atgaacacct aatttttgtg atgcttgttg tcctcttaca tcattccgag
     7441 aacctatata aactttgtaa cccaatgctt taagtgcctc tgcacttgca tagcctaacc
     7501 ctttattgcc tcctgtgatt aacacaattt tagtcattac gtcccacctc atctaaataa
     7561 atgtttaata aataatttct gtacgcttca attgaaatat ggcgatgctc tatttggaag
     7621 gcaaatacac tagttgataa tgattgcaac agcatatctg ttttgaactt cgtgtaagtg
     7681 tcgtcatcgc ttttaaataa gtcataataa aaatcaaata attcttgata aaatgcgctt
     7741 tggtaaaaac gtaatttatt gttgcctgct tcaatacatt gcagtagtgc cttattatcg
     7801 attttaaatt gtaaaagata atctaacgac acttgcataa cctcataatt agaatgatag
     7861 tcatctttaa tttgcttaaa atgagtgata aaaatatcaa ggtctctttg tatgacgtag
     7921 tagcataaat cgcttttatc tttgaaatgt cgatacaatg tccccatacc gatacctagt
     7981 tctttagcaa tacgattcat actaatgttt tcaacgcctt cttcatcaaa aagtttgtgc
     8041 gctatttctt caattcgttg cctattctct tttgcatctt ttcgcatgat tacacctact
     8101 taaaattctc taaaattgac aaacggataa ctctccgttt attataaaac gtgttaagaa
     8161 agttagcaat gaatttgcaa taactattaa atatcataaa agaaaagagt gttgataatg
     8221 tctttaaata aagagcaaag acgcatcaca gctgaagagt tgcaagcaca tttcgaagca
     8281 tctaccttat cggttcaaat gattgctgaa aaactgaatg tcactacaga agatgtggaa
     8341 aaagtattag ctatgacagc gccactaggc atttttagtc atcaattaca acgatttatt
     8401 catttagtat gggatgtcag agatgtaata aacgacaata ttaaaggaaa tggacaaaca
     8461 ccagaaccat atacgtattt aaaaggtgaa aaagaggact attggttttt aagataaaaa
     8521 gaggagtgga tctaaaatga aagatatttt agtaataggc gcaacaggga agcaaggtaa
     8581 tgcagttgta aaacagttgt tagaggatgg atggtatgta agtgcattaa ctagaaataa
     8641 gaataatcgt aaactttcag acataggaca tccgcattta tcaatcgttg aaggtgatct
     8701 gagtgataat gttagtttgc aatcagcgat gaaaggtaag tatggcttat acagtattca
     8761 gccaatcgtt aaagatgatg ttagcgaaga attaagacaa ggtatgaaga taattgaaat
     8821 agctgagcaa gaaaatattc aacatattgt gtatagtact gcgggagggg ttaatcgaaa
     8881 tcgaactggc ccacactttg aagtactagc aaaaatagaa aatagattaa tggaatctaa
     8941 tataaatgca acagttatca aaccatcatt ctttatggat aattttttac gcattgctaa
     9001 agtcgaagat gaacgtatta cattaccaga atttattaat ccgaatataa aattcacaat
     9061 gatttcttct attgatatag ctaaaattgc atcgtatatt tttgcacatc cacaaagctt
     9121 tacgcatcaa tcaatagaga ttggttccga tgaagttaca ttaagtgaag cagcaacaat
     9181 ttttagtgaa gtgacaggta aatctactgt tatagaagga gaatttgtta gtggtgttgc
     9241 agaaaagcaa tggttggaag aaaaaggtta tgaagtagac tttgaattaa tggctgaaat
     9301 aaatccaaca agattatcat taagtgattg gcttaaagtc caaaactata ataagtaatg
     9361 aatggggtgc aatcatgatt caatcaatgt ggtttaattt gcatgtgcaa gatttagaaa
     9421 agagcgcaca gttttataaa gcgttaggat ttaaaataaa tagaaaccca caaatgttag
     9481 ataaaatggt cggcattcaa ataggtcaaa caaccgtaat tttaatagaa aacaagcatt
     9541 ttcaaaatgt aagtcagcaa agccttaata ctgaaccaaa tgaagtgatg atttctctag
     9601 gtgtgaacac aaatgaagaa gttgaccagt tggtgaataa agtgaaagaa gcgggtggca
     9661 cagtcgttca agaaccaaca gtaagccaag ggttttatgg agcaatgttc aaagatcttg
     9721 atggacacca ttttaatttt ttggtctgct aaaatgaaat tctcaaaaga ataaataaaa
     9781 agcccataca gagatgaagc gtaatgtgca ccatggtgcg acacattagt gtcttcaatc
     9841 agtatgggct ttgtttatga ggtcagttgg caccttaagt gcaatacctc gtgagcaaat
     9901 attaattttg aatataaccg tattgttggc gaagatcata aactttatct ggtgtgacat
     9961 cctgaccgat tggatcaacc acttttattg tggaaaatgt tgttaatact tgtcctcgga
    10021 tcatgcttaa gtagtcttga cgcaatgcat gttgttcttg tttttcagct acagtaagtg
    10081 gttgtacttt ttctttattt gcaagttcat taattctatc taaaattttc atattgtcat
    10141 atcctcctta gaatcaagtg ttgatatatt ttgagattat ttttatatgg taatttatca
    10201 acacattaag taatgttttt agtttctcgg tcttttgacc agcagatttt tggaattaaa
    10261 ataagggatt tgagacacga ttttactagt agtacgatgg caaagtgatg ttattgaagt
    10321 gtcgtttgat atacatgtct gcaatgttgt tttataagac aggatgacgt aactgagcaa
    10381 agaaatgagc attgtcgaat gacatcgtgt gtcttattaa aattgcaatg ggcatagcat
    10441 aggtatattc acagtcgatt ttcttgattt ctacattatc ataatacgta gtgtaaaagt
    10501 tatacgacag tcgatagtcg attcgctttt taccacgagc aattattatg tgttgcttca
    10561 acgtcgactt taatttattt gtaatgtatg tcactacacc cagactactc tgattaatat
    10621 ttaatatatt atgcgtgcgt tcaaacccct gaggttcatc tgtctcgctc acgtcaatcc
    10681 ctcctatctt agtatttaaa caacgcatat caactatgca acatatgttg tttagacata
    10741 ctatactact atgtcgtacg tttgccaata aacacgcaca tatcatttgt tgcctaaaca
    10801 acatattgcc tgaagtgttg tttaagtacg caaaaacaaa aatgcttgtc actaaaatct
    10861 atatgtttta atgacaagct tattttcatt cagttaacga ctgctaatat gacattgtag
    10921 agtataggac gttgatttat gtcctagaca cggctaatca atcgtaatac gttaaatttt
    10981 attgtgtgta caagtatgaa cctaagtcac atagtgattt caagatgcat gactaaatac
    11041 caagttttat taaaaatgtg ctatatgatg aattaaaaat ggaaggttgc gatcgataaa
    11101 cacatcggct gtctcttgac tcgtgttaaa ccaatgtaat gctaagcctg agataccagc
    11161 tgctaaaaaa ctcgcactat ttacaatgat atctcgttga tgctccggat agctgttttc
    11221 taacataatc gcaaaaatat cttctaattc attattaata cgttgatgtg ctttgttgca
    11281 aaatgcttca ctatttaatt cgcaagactt tgcagcatct ttgatataac tcgcaattga
    11341 aatgaaaata ttacgcagaa ccttttcatt aatgacatta gaaatgctca aattatcatt
    11401 caagtctttt aaaatggtta cagataatgt gtagtctagg aggtcttctt tatcagtgaa
    11461 atgagcgtaa aatgttgcac gatttagcgt agcaatatca gtgatatctt taactgtaat
    11521 ttgagataat ttcttttctc tagaaatttt aagaaaagca tcgacaagca attgtttggt
    11581 tctaacgatt cttggatcta tggatttact ttccatcatt tgttcactac ctaacatctg
    11641 ttgttttaac acttattatc agtatcttct attatattgt caattgtgtt gatgatatgt
    11701 aagtgctgat acgctcctaa caaataatgt acctttttgg taagcatttg aatgtctttc
    11761 ttctattgta ttaatggatt ttaaaaatga agataaaata aaagtgtatt gtaacaatat
    11821 gtttgaaaaa ttgccaattt ctttatctga aaattcagaa ttttgtgata tactattttt
    11881 catagtcttg agttatagat gatactgcta gcctaaatat aacaacatcg gcatgcacat
    11941 cttagaattt aagctagtgg cagcttataa cttaaggctc tttattattt gcatacagat
    12001 gtatatacat aaatggaagg atgatattat gacatcaatt aaaacaatta cacccaaaga
    12061 ttttatattt agagttttat caggtgtcgc cataggtatt gttgcgggtt tagttcctaa
    12121 tgcgatactt ggagaaattt ttaaatactt tatggattat catccgattt ttaaaacgtt
    12181 attaggcgtt gtcgttgcaa ttcaatttac agtacctgct ttgattggtg cattggttgc
    12241 catgaaattt gacctatcgc cattagcaat tgctgttgtg gcaagtgcag catatgtagg
    12301 aagcggtgca gcgcagttta aaaatggtgc ttggatgatt acgggtattg gtgatttaat
    12361 taatacaatg attacagcag cgattgcagt gttattcatt ttacttattc aacatcgcgt
    12421 aggtagtatg gcattgattg tattcccaac agttgttggc ggtatttcag gtgcaattgg
    12481 tgttttaata ttgccgtata cgaaaatgat tacaacagcc atcggtaata tggttaatgg
    12541 ttttacagaa ttacaaccga ttgtcatgag tattttgatt tcaatggtat ttagtcttat
    12601 tattatttcg ccactatcaa ctgtggccat tgcctttgcg ataggtatta caggtttagc
    12661 agctggatca gcgtcaattg gtatctccgc gactgaagca gtattgataa ttggtacaag
    12721 taaagtgaat cgtttagggg tgccgttatc cgtcttcttt ggaggcgtta aaatgatgat
    12781 acctaatatg gtgaaatatc caattcttat gttaccaata ttaacgacag ccattgtatc
    12841 aggtttggta tcagcattag taggtattca tggaacgaaa gagtcagctg gatttggttt
    12901 tataggaatg gtaggaccga ttaatgcatt taaatttatg gaggttgatt ctgcttggtt
    12961 gagtgtctta ctaattgtag tagcgttctt tgttgtacca tttgtaacag cttggcttgc
    13021 agatatcatt tatcgtaaag tgtttcgttt atatactaat gatattttta aattcatggg
    13081 ataataacag tatgtttttt gaaaatatga gaccaaaaat tatttaatgt tattgtgaat
    13141 tgcgagaatg aagttgaaca atataattga gctgcagttt atttgcgcta ataaaagata
    13201 agacaattca caaaagatgt gtgttttaaa actttgaata tacaacaata atagcaatca
    13261 atacatgtgt agaaacatat ttgttgattg ctttttaatt gcgtttaatg tacgaaaata
    13321 agagaagtta catgtcagat atattacagc tatgtaacaa aaatacaatc tgtaatatta
    13381 cgaaagctgt aatagttgag ctaaatatag taaaaatctt atgtgttaaa gactatttca
    13441 agggttgatt ggattctaaa gggcacatat ttcacattac agtataaaac agccatattt
    13501 caacattaca aataacactt gatattgtaa tgttttgtaa agaaagtgta atttactggc
    13561 tggttttttg tgatatagtg agtaccgttg aaagaaataa aagacaattt aaacttgcac
    13621 tacgacattc aaattcaaaa tataattact aaaaacataa acttacaatt attactggag
    13681 gattttttac tatgaaaaag acaattatgg catcatcatt agcagtggca ttaggtgtaa
    13741 caggttacgc agcaggtaca ggacatcaag cacacgctgc tgaagtaaac gttgatcaag
    13801 cacacttagt tgacttagcg cataatcacc aagatcaatt aaatgcagct ccaatcaaag
    13861 atggtgcata tgacatccac tttgtaaaag atggtttcca atataacttc acttcaaatg
    13921 gtactacatg gtcatggagc tatgaagcag ctaatggtca aactgctggt ttctcaaacg
    13981 ttgcaggtgc agactacact acttcataca accaaggttc aaatgtacaa tcagtaagct
    14041 acaatgcaca atcaagtaac tcaaacgttg aagctgtttc agctccaact taccataact
    14101 acagcacttc aactacttca agttcagtga gattaagcaa tggtaatact gcaggtgcta
    14161 ctggttcatc agcagctcaa atcatggctc aacgtactgg tgtttcagct tctacatggg
    14221 ctgcaatcat cgctcgtgaa tcaaatggtc aagtaaatgc ttacaaccca tcaggtgctt
    14281 caggtttatt ccaaactatg ccaggttggg gtccaacaaa cactgttgac caacaaatca
    14341 acgcagctgt taaagcatac aaagcacaag gtttaggtgc ttggggattc taattccttc
    14401 ataataaata aaagtaatgt ttagacatta aatatatagc tcttcccata gaaattgggg
    14461 agagtttttt tattaattaa aaatacattt tttaaaaagt ggatattgaa gttagggaaa
    14521 tttattggac tttattgtat tactgctaaa atacttgtct ttattaagtg ccaagtggaa
    14581 tgaaacttca tttgaggctg tactgagtac actagataca aactaaacgc acattttata
    14641 attaaattaa atgaaaaagg agataaaaat ggctataaac ataattgaat ataataggtc
    14701 ttataaggaa gagctaatcg agtttatttt atcgattcaa aaaaatgaat ttaatataaa
    14761 aattgataga gatgatcaac ctgatttgga gaacatcgaa cataactatt tgaataatgg
    14821 agggcaattt tggcttgcaa taaacaatca tcaaaatatt gttggtacta taggtttgat
    14881 tagactagat aataatatgt ctgcacttaa aaaaatgttt gttgataaag gttataggaa
    14941 tttaaaaata ggtaaaaaac ttttagataa agttataatg acatgtaaag aacaaaatat
    15001 cgatggtatt tatttaggaa caattgataa atttattagt gcacagtatt tttattctaa
    15061 taatggcttc cgtgaaatta agcgaggaga tttaccaagt agttttccta aattagatgt
    15121 agataatagg ttttattaca gaaatttaaa agattaattc gatgcaataa taattgatat
    15181 gacaatctta gtaatattca ttttaaaagt gacacatagc gtttaaatat aaagtctgac
    15241 gagatatatc gtttggttta cattagagga taggctgcat ttcgcttttt tttcaataca
    15301 tgttaattta
//
LOCUS       DAKTGS010000062        15083 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.062, whole genome shotgun sequence.
ACCESSION   DAKTGS010000062 DAKTGS010000000
VERSION     DAKTGS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.062"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(31..1131)
                     /locus_tag="O0286_001552"
     CDS             complement(31..1131)
                     /locus_tag="O0286_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCY0169932.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            complement(1134..1811)
                     /locus_tag="O0286_001553"
     CDS             complement(1134..1811)
                     /locus_tag="O0286_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCY0169933.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(1942..3048)
                     /gene="rpoD"
                     /locus_tag="O0286_001554"
     CDS             complement(1942..3048)
                     /gene="rpoD"
                     /locus_tag="O0286_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCY0169934.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(3272..5071)
                     /locus_tag="O0286_001555"
     CDS             complement(3272..5071)
                     /locus_tag="O0286_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0169935.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(5132..5950)
                     /locus_tag="O0286_001556"
     CDS             complement(5132..5950)
                     /locus_tag="O0286_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCY0169936.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(5961..6584)
                     /locus_tag="O0286_001557"
     CDS             complement(5961..6584)
                     /locus_tag="O0286_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0169937.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            6919..8310
                     /locus_tag="O0286_001558"
     CDS             6919..8310
                     /locus_tag="O0286_001558"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCY0169938.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(8460..9206)
                     /gene="recO"
                     /locus_tag="O0286_001559"
     CDS             complement(8460..9206)
                     /gene="recO"
                     /locus_tag="O0286_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCY0169939.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(9234..10133)
                     /gene="era"
                     /locus_tag="O0286_001560"
     CDS             complement(9234..10133)
                     /gene="era"
                     /locus_tag="O0286_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCY0169940.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(10134..10538)
                     /gene="cdd"
                     /locus_tag="O0286_001561"
     CDS             complement(10134..10538)
                     /gene="cdd"
                     /locus_tag="O0286_001561"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCY0169941.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(10549..10893)
                     /locus_tag="O0286_001562"
     CDS             complement(10549..10893)
                     /locus_tag="O0286_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0169942.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(10896..11363)
                     /gene="ybeY"
                     /locus_tag="O0286_001563"
     CDS             complement(10896..11363)
                     /gene="ybeY"
                     /locus_tag="O0286_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCY0169943.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(11364..12311)
                     /locus_tag="O0286_001564"
     CDS             complement(11364..12311)
                     /locus_tag="O0286_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCY0169944.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(12615..13313)
                     /locus_tag="O0286_001565"
     CDS             complement(12615..13313)
                     /locus_tag="O0286_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCY0169945.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(13330..14319)
                     /gene="floA"
                     /locus_tag="O0286_001566"
     CDS             complement(13330..14319)
                     /gene="floA"
                     /locus_tag="O0286_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCY0169946.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(14337..15020)
                     /locus_tag="O0286_001567"
     CDS             complement(14337..15020)
                     /locus_tag="O0286_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCY0169947.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT         4681 a         2833 c         2070 g         5499 t
ORIGIN      
        1 ttcaaaattt agaaatcccg agattttagt ttaaatatat tgaaatggat ctgtattaat
       61 tgtagatgct tcaacatcta aatttatttt ttctgtattg aaccaattca ttagtaacgc
      121 ttttaaacct tctttcatca catattcgct gtaatgatta atatcaatta aattcacacc
      181 atgaatttta gcatctaagg catcatgatg tttaatatca cctgtaacaa agacatctgc
      241 gccttgttgg acagcttgat attcatatcc aatacctgaa ccaccaataa ttgcaatacg
      301 tttaattttc tgattagact caccaacaaa acggacactt gggatattta atttagattt
      361 aatatcagct gcgaaatctt ccaatgtcat ttgattatcc acttctgcca taacgccaag
      421 tccataaagg gatgtttgtt ttatctcaat aaaatcaaat accggtgttt catatggatg
      481 gtattgttta attaattgct cagcccttga cttttgatat gcatctatca taaattcaat
      541 tttaacttca tctacatatt caattttatc aatttgtcct attgttggat tagcttcacc
      601 aactggtttg aattgccctc ttccttcact ttcaaagaaa caatattcat aattaccttc
      661 ttgcgctaat ccattttcac taagcttatc tttaaatggt ccaacattat ccttaggtat
      721 atatgtttga actttatagt atacatcttg ttgattattt attattgaaa tgttcttcaa
      781 acccatcgcc ttcgccaaca tcatattgac accatacgga tttacatcta aatttgtatg
      841 catcgctatt aaattaatgt catgttgaat tagttttcta atgatcaaac cataaccatt
      901 agcttttaat gatgttacgc ctttaaagat tagaggatga tgactaataa tagtattata
      961 acctttttcg attgcttcat ttactacttc caacgtacag tctaatgctg ttaaaacacc
     1021 agtaacttca acatcttcat cacctattaa caatcctaca ttatcccaag attcagcagt
     1081 actaaatggc acatgatgat ctaacaatgt cattaaatca gctattttca ttactataac
     1141 accctttcaa ttacagcaat ttcgtcatta atttgagcta aacgttgatg atgttgttca
     1201 gtattgagtt tcgatttaat atgataaagt gcttctaact ctctttgcca tttttttata
     1261 aaatattcat ttttgttgtt cagcaatttt ggtccgaatt tcaattcatc agatgatagc
     1321 tctattaatt ctgtagaata ttctgctaca acaatttcat aaatatggcc tttttcttcc
     1381 attattattt catcaattat ttcataattc aattgttgta atgtttgtct taaattttca
     1441 gtttggatat tactttgtaa aatcaacctt ggatgttgac ttaacttatc ttgcccatct
     1501 tttaaaattt tagcaataag tggtccgccc ataccacaaa ttgtgatatt atcgattacg
     1561 tcctcaggtt gaataacact taagccatcc cctaaacgta catcaattct atctactaat
     1621 tggtttgcag ctacattttt cacagcagct tgaaaagggc cttgaataac ttctccagca
     1681 ataccgcatt cgcataaatg gttttgaatt gcatagattg gcaaataagc atgatctgag
     1741 ccaatatccg cgattgtacc ttgttttaaa aatcgactta ccgtcgttaa tcggttattt
     1801 aacgaaatca ttttattctc ctttatgaaa aaagaggttg aacattattc aatgtaccaa
     1861 cctcactcaa gttaaatctt aatctaaaaa taaataagcg cttattcatg tcttggtatc
     1921 aatcattaaa attgatataa attaatccat aaagtctttc aaacgtttac tacgacttgg
     1981 atgtcttaat tttctaagtg cttttgcttc aatttgtcga atacgttcac gtgtaacacc
     2041 gaaaacttta ccaacttctt caagtgttct tgttctgccg tcatcaagac caaatcttaa
     2101 tcgtaataca ttttcttctc tatcagttaa tgtatcaagc acatcttcta attgctcttt
     2161 taataattca taagcagcat gatctgaagg actttgtgct tcctgatcct caataaagtc
     2221 tcctaaatga ctatcatctt cttcaccaat tggtgtttct aatgaaacag gttcttgcgc
     2281 aatttttaaa atttcacgaa ctttttctgc tggtaaatcc atttcttcac caatttcttc
     2341 tggtgctgga tctcgaccta agtcctgtaa taattgacgt tgaacacgaa ttaatttatt
     2401 aattgtttct accatatgca cagggatacg aatcgtacgt gcttggtcag caattgcacg
     2461 agtgattgct tgtctaatcc accatgttgc atatgttgaa aacttaaatc ctttgttaaa
     2521 gtcaaatttt tcaacagctt taataagacc catattacct tcttggatta aatcaaggaa
     2581 taacatacca cgacctacgt atcttttagc aatacttaca actaaacgta agttcgcttc
     2641 tgcaagtctt gattttgcta cttcatcacc ttgttcaata cgtttggcta attcgatttc
     2701 ttcttgtgca cttaataagt taacacgccc aatttcttta aggtacatac gaactgggtc
     2761 atttatttta acacctggag gggcactaag atcacttgga ttcagtttct cgtcagtatc
     2821 tgaactatct ttttcattaa ctagtgaaat atcattatca tttaattgat caaagaaatc
     2881 atccatttga tcagagtcga tatcaaaatt ctgaagtttt tcagcaattt cttcatgact
     2941 taaatgaccc tcttttttac ctttttcaat taattgcttc ttaacatctt ctaatgttaa
     3001 tgtcggatca attgtttgtt ttttaatttt aactgtgtta tcagacatga aacggcctcc
     3061 cgattttaaa tatgaacatt cgaaatttat tcaatattgc tattttaaac taaattctta
     3121 attaattcat ccatattttt aattttattt tacaaattgg aactaatccc aatatttatt
     3181 tttcaatagt gttgtttaaa tgcggtaact atcatttacc cttatacatt ttgacataat
     3241 cattattcgt attattcttt aaaatcacat gctacatgcg ttctttattc ttagcaacaa
     3301 tttgctgtaa atagtatttt tgtaattcta catcgccaat ccttgtagct tcccttaatt
     3361 tatgattcaa tgactcaatt gtttcttgtc ctttttcatt aataacattg acataatcat
     3421 caatttcatt ttcatatggt tcgtcattca aattatattg ttctaagcta attagtgttt
     3481 ctctcaactc atttgaatta acatactgca cagcatcact gatattatat tgatcatttt
     3541 ccgcataaaa atcatgtaag acttcgaata catatttaaa atgctgattt gtgaagttat
     3601 ccttatcaac actttcataa taatttaaaa atgtatcttt atctctcatt aaatgtttta
     3661 aaaatgctcg ctccgctttt tcttgacggc tcaaattgtc aaattgtgcc ataccaattg
     3721 gctcaggttc aatgtaaccg ccatactcat cttctggata ataattggct ggtgcttgat
     3781 tgaattgtat ttcgttagct aattgctcag gactaacatt gaaaaatggt gcaacatcat
     3841 ttaaagcctt ttgttgcaaa atcgatgatt tcataagcga aatatcatga cttagttctt
     3901 tcaaataacg ttcatatgaa aggtcattat gtgcaatttc atcttttaat atactcactt
     3961 tataatgtgc aaatgacttt ttgtcatttt ttacaaaagc agtaaatgca tcgttgccat
     4021 acttaccaat gtattcatcc ggatccatgc ctgatggcaa ttgtataaca aatacattta
     4081 gcccttgctg taacaaattt tgacctgttt taagtgttgc ttcactaccc gcaaaatccc
     4141 catcaaacat taatgttata tttgatgtta actttcgtat aaaagtaata tgttcatctg
     4201 acaactgtgt acccattgtt gcaacaacgt ttttcaagcc agcagtatca gattttataa
     4261 catccataaa accttctagt aatacgattt catctaattt tctaattgat ttacgcgctt
     4321 tatctaagtt gtataacaac tttctttttt gaaagatagg tgtttcagga ctatttaagt
     4381 attttggttc ttgaccggta tatgttcgac ctgaatatcc aacaattctt ccttgcgcat
     4441 ttttcaaagg aaacataata cgatttcgaa atctatcgta ataactgaaa ttttcttcgt
     4501 tacgtgataa taatccggct tcatatgcta attcaatatc gtaacccttt ttttgaagaa
     4561 aatcatgaca aaaatgtgag ctatcgggtg caaagccaat gcctcgctct ttaataagcg
     4621 catctgtaaa accacgttct tgtaagtacg ttaatgcttg ttcgccttcg actgtctttg
     4681 ttaaagcgta ataataaaat tcttgtatta actcatgcat ttcaatcatt tgtaaatcat
     4741 cagaagcaat ttgaacattt gagttagatt gtgttgcctc aatatctaca gcaacattaa
     4801 ctctatcacc taattcttta accgcttcaa caaatgatat gtctttaatt tcttgagtaa
     4861 attgaaaaac attgccacct tttttacaac caaaacaatg acaaatttgt ttatcttcag
     4921 aaactgtaaa tgaaggtgtc ttttcatcat gaaaaggaca caaacctata taattgcgtc
     4981 ctctcttttc taattttaca tattcactta ccaagtctaa aatgtcggtt ttatctttta
     5041 tttcattaat gatcgattga tctattcgca aattaaatca cctattaagc attagttata
     5101 ctattatacc taattttcga caaaaatgaa atcatttcga tttattttgt tcaatataat
     5161 ggattatatc gtttgctgtt tcttcgattg ctttttgaga aacatcaatg acaggacatc
     5221 caatttcact tacgatttct tcaaagtaat tcaattcttc ttgaattcgt gcttctgttg
     5281 catatcgagc tgtgtcacct agtcctaatt gttttagtcg ctcttttcta atgcgattta
     5341 atttttcttc acttatttta agtgcgatac atttctttgg attaatatca tataagccat
     5401 ctggcggtgt cacttctggt acaatcggta cattcataac tttgtaactc ttatgcgcta
     5461 aatactgaga taatggtgtc tttgaagttc tcgaaatacc aagtaaaaca atatcagctt
     5521 taggtaatcc tttaggatct ttaccatcat catatttaac tgcaaactct atcgcatcaa
     5581 ttttcttgaa atatgcatca tctaatctat gaacgatacc tggctcatta taaggttttt
     5641 cttcaaccga agcagataat aaatccatta atggccccat gatatcgaca gacttcaatt
     5701 ggaattctgc tactttctca ctcatatatt gcttcatttc aggtttaata agtgtataaa
     5761 caatgatagc atttgtatct tttgcaactt gaatcacttc atcaacatct tcaaaagatt
     5821 caatatatgg atatcttaat aattcatttt tacattgctt aggattgaat tgtgaaatac
     5881 ctgccctagc aactaactct gccgtttcac ctatagaatc tgaagctacg ataattttaa
     5941 ttttttccat tacttctcac ctattcttta tataatgcta ctaataactt agctattgtt
     6001 gttttggaaa ttcttccaat tacttcatac ttttgattat ctttttttct tacaattgga
     6061 atcgaatcaa tttctttttc aatcattcta tctgctgcgt atatgactaa ttcgctttcc
     6121 tctaaataag tgacattagg catacgtgtc atatttacac tgataggtac tttatgaata
     6181 tctgctccaa tcattgaagc tcttaataaa tcttttcttg aacacacacc aacaaaatcg
     6241 ttatcttcat taataataaa taatgtactt acatcttcta aaaaaattgt acaaatagcg
     6301 tcataaactg ttgtattctc tcttagcaca acaggttgag acatatagtc cttaacctca
     6361 aattgtcgaa gtttttcatt aaaaaatttg ccttttgatt tacctgaata ataatatcca
     6421 actcggggac gcgcttctaa aaaacctgac attgttaata tcgctaaatc tggtctaagc
     6481 gttgctcttg ttaaattcaa cttatctgct atttgttcac cagtaatggg tcctttagtt
     6541 ttaacaattt cgatgattcg ttcttgtctt tgactgagtt ctataggtct tcaccccttt
     6601 tttattcacc tattattata ccgatatttc tttcatgtca caaacacatt aattttactt
     6661 gcctttaaat aatctatcaa ttagaataaa cgtacagcga gttaaggata gtgtaagctt
     6721 aacaataaga ttggcgcaac gaatcatttt aaaataaaag cgagtgacta cactaatttg
     6781 ggtggaaccg cgggttaaat acgtaactta ttttgtatta tatacaattt aaaatattaa
     6841 ctcgtcccat ggaagtaaag catatttact atgctgttat tttccatggg gcgttttcat
     6901 gtatgaggag aggtaattat ggcaaaagat atggatacaa ttgtttcatt agcaaaacac
     6961 agaggttttg tgttccctgg tagtgatatt tacggtggtt tatcaaacac atgggattat
     7021 ggtcctttag gtgttgaatt aaagaataat gttaaaaaag cttggtggca aaaattcatt
     7081 acacaatcac cgtttaacgt tggtatcgat gctgcaatct taatgaatcc aaaagtatgg
     7141 gaagcttcag gacacttaaa caacttcaac gacccaatga ttgataataa agatagtaaa
     7201 attcgatatc gcgctgataa attaattgaa gattatatgc aagatgttaa aggtgatgaa
     7261 aacttcattg ccgatggttt aagttttgaa caaatgaaaa aaattattga cgatgaaggt
     7321 attgtttgtc ctgtaagtaa aactgctaac tggactgaaa ttcgccaatt caatttaatg
     7381 tttaaaacat tccaaggtgt aactgaagat tctacaaatg aaattttctt acgtcctgaa
     7441 acagcacaag gtatttttgt aaactataaa aacgtgcaac gttcaatgcg taaaaaatta
     7501 ccatttggta tcggtcaaat tggtaaatca ttccgtaatg aaatcactcc aggtaacttc
     7561 attttcagaa caagagaatt tgaacaaatg gaacttgaat tcttctgtaa acctggagaa
     7621 gaaatcgaat ggcaaaatta ttggaaaact tttgcaagtg actggttaac aagcttaaat
     7681 atgagcagtg aaaatatgcg tttacgtgat catgatgaag atgaattatc tcattactca
     7741 aatgcaacaa ctgatattga atataaattc ccatttggtt ggggtgagtt atggggtatc
     7801 gcaagtcgta cagacttcga cttacgtaaa catgctgaac actctggtga agatttcaga
     7861 taccatgatc cagaaacgaa cgaaaaatat attccatatt gtatcgagcc atcacttggt
     7921 gcagatcgtg taacattagc tttcttatgt gatgcatatg atgaagaagg cgttgaaggt
     7981 agtaaagatg cacgtacagt tttacacttc catcctgcat tagcaccata taaagcagcg
     8041 attttacctt taagtaagaa attatctggc gaagcgatta agatttttga gcaattaagt
     8101 tctaaattct caatcgattt cgatgaatca caatctatcg gtaaaagata ccgtcgtcaa
     8161 gatgaaatcg gtacacctta ttgtgtaaca ttcgactttg attcattaga agataatcaa
     8221 gttacagtac gtgacagaga ttcaatggaa caagttcgta tgccaatctc agagttagaa
     8281 gctttcttaa ctgaaaaaac aaaattctaa ttaaaaagac tttccaccat atgtggtggc
     8341 tatataattt ataaaaagag atactggtta gtaaaattgc cagtatctct tttctattca
     8401 aaaaggtatt caaataaaca catctatgtg gctacttgaa taccttttat tattttatgt
     8461 tattgttcca atctttttaa ttggttgatt agtttctgac ttttaaaaaa catacctgca
     8521 tattcacgat ataacattaa aatgatatct gacatttcat caataatttc ttgatggata
     8581 ttcaatgaat tcattttatc tatcggtaat ttttgtaata catctaataa atatagtgtt
     8641 ttattcgata atataactgc atgtacatct ttagaagcct cttgccttga aatcgcaccg
     8701 tcaaacttaa aactataacc tattaaatct gcttgtgtgt cattaccact cacagcacag
     8761 cgattaaatg atgcagtaaa accaaatcgt ttcatgcact ttaacataac tacgactgac
     8821 attaactgtg cagatgtacc tgattctatt ttttcaagaa caaattgtaa taattgatag
     8881 ttatatggtg caatgtcacc ttcatccatt gagcgctcaa tagtttcagc tgccagagcg
     8941 gcataactgc ttacgtaaag gtccatttgt aatttataat gttgactaat aacatctaca
     9001 gaatttaacg ttcccatacc tcgccactga ttgtaaataa acaaaccata aacaaacaat
     9061 tgcgtttgcg cttgtaaacc cgtcttaact tttttagcac gccttgccat aagtggtact
     9121 tttgcaccat gctcatttaa aatcgtgata attttatcag attcaccata atcaactgct
     9181 ttgatgataa tccctttttg gcgcattaac aattatcttc accactttta agattaatct
     9241 tggtcttcaa cataaccaat ttggcgaata aagttaactt tgtttcgcca gtctctttga
     9301 actttgaccc ataattctaa gtaaacttta gagcctagaa gcatttctat atcacgtctc
     9361 gcacgttttc ctacttcttt taacttttta ccgccttttc caatgacaat tcctttttgc
     9421 gaatctcttt caacatatat agttgcttcg atatgaacac gatcttcgct ttctttaacc
     9481 atacggtcca cattaacacc aatcgcatga gggatttctt cacttgtaag atgaaggatt
     9541 ttttcacgaa tgatttcacc cactacaaat tgttcaggat ggtctgaaat ttgatcatct
     9601 ggataatatt taggtccttc gggtaaatac gtctttaaaa catcaataaa atgatcgaca
     9661 tttagccctt ctaatgctga aataggtaca atctctgtaa agtccatata actttgatat
     9721 tcttcaatct ttggcattaa ttcatctgga tgcactaaat ctattttatt taatactaaa
     9781 aatactggtg tcttaacatt tttcaacatt tctataatat attcatcgcc tcgtccaatt
     9841 tcctcattgg cattaaccat aaacatgatt gcatctatct cagataatgt atttttagcg
     9901 actttcatca tatagtcacc taatttgtgt ttaggtttat gaatacctgg cgtatcaatg
     9961 aatataattt gcgcgtcatc tcttgtcata acaccttgaa ttttatttct agttgtttga
    10021 gctttatcgg acatgattgc tattttatgg ccgatcactc tattaacaaa tgttgacttt
    10081 cctacatttg gtctacctat aattgaaaca aatcctgatt tatgttctgt catttattct
    10141 aaatcctttc ctgaaaatcc aaatggtagt aactctgcga ctgtcatcat aaccatatct
    10201 cctttatgat ttgtcatata cacaggcata tcatcatcac ataattcctt caaaacttga
    10261 cgacatgcac cacaaggtga tgacggttta tctgcatcta cggttacagt tattgattca
    10321 aaatcacctg gtctgtatcc ttgagaaatt gccgatacca aactagctcg ttcagcacat
    10381 atcgataatg gataagaagc attttctaca ttggtaccat aaaaagttct accgtctttc
    10441 gtttttaaat aagcccctac tttaaattga ctgtatggcg aatatgattc ttgttgtgct
    10501 tttctaactt cttgaaaata atgaggttga taactcatat atgcctccct aaaataacgc
    10561 tataaaatgt ggtaaaaata ctattaaacc tataataaat gctaatattg aaactataag
    10621 tacactaaaa gccgcaatat ctttagcgta tttagctaaa tcatgatatt caacggtcac
    10681 taaatcgaca acatattcaa tagcagtgtt taaagcttca acagtgagaa ctaatgcaat
    10741 agcaatgagt ataaatatcc actcaatccg attaatattt agtacgagac caaagacaat
    10801 agcaacaatc attgcaaaca catgtaaaag aaatttatag tctttttgaa ttaagatttt
    10861 cagcccatca agtgcatatt taaacctttt cataattagt ctcgtgttaa tccatatgcg
    10921 tttaatattg tatcttgtcg accaaacatt tccttttcat ccgcttcagt catatgatca
    10981 taacctaata gatgcaaaaa tccatgtaat gctaaaaatc ctaattctcg ttcaaaagaa
    11041 tgtccgtaat tgtttgcttg ttcttgcgct acatccgtac agataattat atcccctaaa
    11101 acacgtggta tatcaagacc actaaaatca atctctggct catcttcttc taaagcaaat
    11161 gagattacat ctgtaacttt atctttatct ctatatgttc gattaatttc ttgtatttct
    11221 tgtttatcta caaatgtaac agaaagctca gcatcgtctt ctatatgctc ttctttttta
    11281 gcaaattcta ataaatcttc aatttgttta taccaagcat ctttaactaa gcctgtgtga
    11341 tcgctaaaat ctatcgtaaa catttaattc tctccttcat aatgttcaat gatcttactt
    11401 accaatggat gtctgactac atcgctctga tctaatttca atatacttat acctttaacg
    11461 ttgtgtaacc tactgaccgc ttccttaagt ccacttttaa cacctttagg taaatcgatt
    11521 tgagtttggt caccagtaac taccattttt gagccaaaac ctagtcttgt taaaaacatt
    11581 ttcatttgcg catgtgtcgt attctgcgcc tcatcaagaa ttacaaatgc atcttctaat
    11641 gttcgtccgc gcatatatgc aagtggcgct atttcgataa tgcctctttc aataaatcgc
    11701 tctgtttgtt cacgcccaag aacagtatat agaccatcat ataaaggtct taaatatgga
    11761 tctacctttt ctttcaaatc tcctggtaaa aatccaagtg actctcctgc ttcaacagca
    11821 ggtcttgtta atacaatacg tttaacagca cctttacgga gttgctttgc tgcataaact
    11881 acagctaaga atgtcttacc tgtaccagca ggacctatac caaatactaa atcattattt
    11941 ttcatggcat taacatatat acgttgcccc atcgttttcg cacgaatcgt cttaccaaat
    12001 gcatctttag ttatctcttc atcatataaa tctaacagat gttgaattgt gttattatgc
    12061 gccattttaa tagctgcttc aacatcttta attgtaatat tattacctaa atcaataacc
    12121 ttcagcaaat tgattaatac tgattccgct ttttctacgt tttctatttt tgtaccttta
    12181 acggcaactt cttgtcctct tgcatggatg acaacatcga aactctcttc aattgctttt
    12241 aaatgttcat cattatttcc aattaaagct tgagattggt tcatatcgtc tatttgtata
    12301 attccaggca tacacgcgct ccttttcata tatcatttat attcattcta tgatgcttta
    12361 atatctactt aattatatca tgcaattgat tgatttaaac atctttgcat tgacttactc
    12421 atttttcttt aatttaaaat aatcatttat acttttcacc ttgtcataaa agcagattca
    12481 attttagact aatcaatttt ttgctgtaat ttaaataact aatatttctc gaaaaattct
    12541 ctttgttttt taatttttct taacgtaaaa aaacaagcaa ctaccattac gatagtgctt
    12601 gttttcaaaa aattttataa ttgttttggt ttagctaaaa tttctgacca tatcatacca
    12661 ttgattactt catctttatc aaattggaac gctgatttag tcaaatcttt ttcgacattt
    12721 tgagaattaa gcagctgctt taatttcaaa cgttttgtac gttcagataa gtatttatcc
    12781 tcaataatat ctctagcacg tttttccatt ttagcaattt gcttttcttt ttctctatca
    12841 atgtcagtac gaatcgtttt aatatcatct ctaagtgatt tttctaattg acgtctaatt
    12901 tcgtcagaat tatcttgtct agaaggtcta tgtttctgct ccaccggttc tgctttaggc
    12961 actgtgattg gtttctctgt catcggtttt gtttcttgtt gttttttagg tgccataggt
    13021 tctacaacag gtttcgattt aggctcttcc tttggaagtt catcgaataa aggtggtaac
    13081 gtatcatcat attttcgttt cgatgatttc ttttcttctt catttaattc ttcacttatt
    13141 tctttaaacg ttcgctcaat ttcttcaaaa aagccacctt tttttggttc attatcggta
    13201 gatgtttttt gaggtggctt ttgattttgt ctatctttat gactattttc gcgcatagta
    13261 gtaatgatag aaatgatcac tgatatgaca aaaattagaa taccgacact catttaatca
    13321 cctctcgact taatgttcag gtgactcatc atcactttga tcagttcgtt tattaattgc
    13381 atttctcatg cctgtatcag cttcgatatt tttcaaatta taataatctt taacactgat
    13441 attacctgaa cgtaatgctt cagccatagc taatggtact tcagattcgg cttcaactac
    13501 tttagcatgc atttcttgta cacgcgcttt catttcttgc tcagttgcta cagccatagc
    13561 tctacgttct tcagcttttg cttgtgcaat atttttgtct gctaatgctt gttcagtttg
    13621 taagtctgca ccaatatttt tactaatatc aacgtcagca atatcaattg ataaaatttc
    13681 aaatgcagta cctgaatcta aacctttgct taaaactgtt ttagaaatat tatctgggtt
    13741 ttcaagtact tctgtatgat gcttactaga accaattgtt gaaacgatac cttcaccaac
    13801 acgtgcgatg attgtttctt caccagcacc accaacaagt cgagcaatat tagctctaac
    13861 tgtgatacga gctttggctt tcacttcaat accgttcatt gctacacctg cgataaatgg
    13921 tgtttcaatg actttaggat taacagacat ttgaaccgct tctaatacgt cacgtcctgc
    13981 aaggtcaatt gcagcagcac gttcgaaagg aagatcaatg tcagcacgtt gtgcagcaat
    14041 attagcgtca acaactctgt caacatttcc tcctgctaga taatgcgatt ctaattggtt
    14101 tgttgttaat gctagtcctg ctttgtgcgc tttaattaat ggcgctataa cttttcttgg
    14161 agatacacga cgtaaacgca taccaaccaa tgtacctata ccaacatgaa cgccagctgc
    14221 taacgctgaa atccataaac caatgggtac aaatgagaat aaaataagta atgcaactac
    14281 tataataact gctattacga taaaacttaa actaaacatg gtatcgctcc ttttaattaa
    14341 tctacttccc tcacaactac tcttgttcct tcaacttcaa ggatttttac cgttttattg
    14401 cgcaaaataa agttgccatc tgaaacagca tcaatacgtt cattttcaca aaaaataatc
    14461 cctgcaggtc gaagatctgt aactgtttga gcagtctttc ctacgaggtg cgagcggtta
    14521 tcatgagaat tgtaacctga ctcagaatta gttgaatctt ttaagataac tttatccaaa
    14581 aacggaatct ttctgttgaa aatcttcact aatatcaccc attctacaat cgttaaaatc
    14641 aaggcaacga taacattcgc aagcataaat agcaaattat caccgagcgt tgttatgctt
    14701 atagttatca gtatcatgcc aataatacca attactgcac caactacaaa taattcaatt
    14761 acaactaata taacgccaat tgagaatatt aagatagaat gcatattgac atttccttgg
    14821 attagaaatc ccaaaaataa aataagtaat gataatgtgg cgataatacc agctgcattg
    14881 atttttttag agtaaagttg atatacaaat cctaagaatg tcaaacaggt taatattaac
    14941 gtaaaaatag gttgaacaat tatattgcta acttgttcaa cccaagtatc tccagccgtt
    15001 gattccaaga tagttgtcat ttgtaaaaaa ttattataac tcacaatttc acctcgccct
    15061 cacattaatt atacatgaaa ttg
//
LOCUS       DAKTGS010000063        15036 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.063, whole genome shotgun sequence.
ACCESSION   DAKTGS010000063 DAKTGS010000000
VERSION     DAKTGS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.063"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            8..1198
                     /locus_tag="O0286_001568"
     CDS             8..1198
                     /locus_tag="O0286_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0169948.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1307..2551
                     /locus_tag="O0286_001569"
     CDS             1307..2551
                     /locus_tag="O0286_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCY0169949.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3054..3983)
                     /locus_tag="O0286_001570"
     CDS             complement(3054..3983)
                     /locus_tag="O0286_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0169950.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4225..5604)
                     /gene="argH"
                     /locus_tag="O0286_001571"
     CDS             complement(4225..5604)
                     /gene="argH"
                     /locus_tag="O0286_001571"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCY0169951.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5594..6799)
                     /locus_tag="O0286_001572"
     CDS             complement(5594..6799)
                     /locus_tag="O0286_001572"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCY0169952.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7150..8481
                     /locus_tag="O0286_001573"
     CDS             7150..8481
                     /locus_tag="O0286_001573"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCY0169953.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8806..9381
                     /locus_tag="O0286_001574"
     CDS             8806..9381
                     /locus_tag="O0286_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCY0169954.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9386..9910
                     /gene="lepB"
                     /locus_tag="O0286_001575"
     CDS             9386..9910
                     /gene="lepB"
                     /locus_tag="O0286_001575"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0169955.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            9926..10501
                     /gene="lepB"
                     /locus_tag="O0286_001576"
     CDS             9926..10501
                     /gene="lepB"
                     /locus_tag="O0286_001576"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0169956.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10661..14137
                     /gene="addB"
                     /locus_tag="O0286_001577"
     CDS             10661..14137
                     /gene="addB"
                     /locus_tag="O0286_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCY0169957.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14138..>15036
                     /locus_tag="O0286_001578"
     CDS             14138..>15036
                     /locus_tag="O0286_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HCY0169958.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDA"
BASE COUNT         5127 a         2286 c         2759 g         4864 t
ORIGIN      
        1 agatatgatg actaaatctg aaaaaattat tgagttaaca aatcattacg gagcacataa
       61 ttatttacca ttgccaattg tcatttcaga agctgaaggg gtatgggtta aagatcctga
      121 aggcaataaa tatatggata tgttatctgc atattccgct gttaaccaag gtcatagaca
      181 tccgaaaatt attcaagcat taaaagatca agctgataaa gtgactttag tttcacgtgc
      241 ttttcatagt gataacttag gtgaatggta cgaaaaaatt tgtaaactgg caggtaaaga
      301 taaagcttta ccaatgaata caggtgctga agcagtagaa acagctttga aagcagcacg
      361 acgctgggca tacgatgtta aaggaattga gccaaataaa gcagaaatca ttgcatttaa
      421 tggtaacttc catggtcgaa caatggcgcc agtttcatta tcttcagaag cagaatacca
      481 acgtggttat ggtccgttat tagatggatt tagaaaagtt gattttggag atgtagatgc
      541 attgaaagct gcaattaatg aaaatactgc agcagtttta gtagaaccaa ttcaaggtga
      601 agcgggtata aatataccgc cagaaggata tttgaaagca attagagaat tatgtgatga
      661 acataatgtc ttatttattg ctgacgaaat ccaagcagga ttaggtcgtt cgggtaaatt
      721 atttgctacg gattgggata atgtaaaacc tgatgtctat attttaggta aagcactagg
      781 tggtggagtc ttcccaattt ctgttgtatt agcagataaa gaagtattag atgtctttac
      841 acctggctca catggttcaa catttggtgg taatccactt gcttgtgctg catcaattgc
      901 tgcattagat gttatcgttg atgaggattt accaggccgc tctttagaat taggagatta
      961 ttttaaagaa caattaaagc aaattgatca tccatcaatt aaagaagtcc gtggacgtgg
     1021 tttgtttata ggtgtggaac ttaatgaaag tgctagacca tattgtgaag ctttgaaaga
     1081 agaaggctta ttatgtaaag aaacgcatga tactgtcatt cgttttgcac caccattaat
     1141 tattactaaa gaagaattgg accttgcact tgaaaaaata agacatgtat ttcaataatg
     1201 tcaaaaagtt gtaacaatca gtttgtaagg gctttaaaaa ggaatacgtt atgttacaat
     1261 atgtttgaaa gcgaaatcat taggtaaaga agaggcgaaa aggatcatga ctgagaacaa
     1321 taatttagta acttctactc aaggaattat taaagaagca ttgcataaat tgggatttga
     1381 cgaaggaatg tacgatttaa ttaaagaacc tttaagaatg ttacaagtgc gtatccctgt
     1441 acgaatggat gatggcacag taaaaacatt cacaggttac cgtgcgcaac ataatgatgc
     1501 tgttggacca acaaaagggg gcgtgcgttt ccacccagat gttgatgaag aagaagtaaa
     1561 agcattatca atgtggatga ctttgaaatg tggcattgta aacttaccat acggtggtgg
     1621 taagggtggt atcgtttgtg atccacgtca aatgagcatt catgaagttg aacgtttatc
     1681 acgcggatat gtaagagcaa tttcacaatt cgtaggtccg aacaaagata ttccagcacc
     1741 agatgtattt acaaactcac aaattatggc ttggatgatg gatgaatata gtgcattaga
     1801 taaatttaat tcaccaggtt tcatcacagg taaaccaatt gtattgggtg gttctcatgg
     1861 acgcgacaga tcaactgcac taggtgtagt tattgcaatt gaacaagctg caaaacgtcg
     1921 taatatgcaa attgaaggtg ccaaggttgt tattcaaggt ttcggtaatg ccggaagttt
     1981 cttagctaaa ttcttatatg atttaggtgc aaaaattgta ggtatctctg atgcttacgg
     2041 tgcattacac gatccaaatg gcttagatat agattattta ttagaccgtc gtgatagttt
     2101 tggtacggta acaaatttat ttgaagaaac aatctcaaat aaagaattgt ttgaattaga
     2161 ttgtgacatt ttagtaccag cggctatttc aaaccaaatt acagaagaca atgcacatga
     2221 tattaaagct agtatcgttg ttgaagctgc taatggacct acaacaccag aagcaacacg
     2281 tattttaact gaacgtggta tattattagt tccagacgta ttagcaagtg ctggtggtgt
     2341 aacggtttct tacttcgaat gggtacaaaa taatcaaggt tattattggt ctgaagaaga
     2401 agttgatgaa aagctacgtg aaaaattaga agcggcattt gatacgattt acgaattgtc
     2461 tcaaaaccga aaaatagata tgagacttgc agcatatatc ataggtatta aacgtacagc
     2521 agaagcagct agatatcgtg gttgggcata attaattatc atatgtgatt taacgagctt
     2581 gggacagaaa acaaagccct aagctcgtta attttatttt agcagtagtt gactgtaaaa
     2641 caatgcccgt gtaacacgct cttttcaaaa atagtcgggg ccccaacaca gaggctggtg
     2701 gaaagtcagc ttacaatagt gtgcaagttg gcggggcccc aacacagagg ctggcggaaa
     2761 gtcagcttac aatagtgtgc aagttggggt ggagccccga cacagagaaa ttagctcctc
     2821 aatttctaca gacaatgcaa gttggcgggg ccccaacaca gaagctggcg aaaagtcagc
     2881 ttacaatagt gtgcaagttg gggtgggacg acgaaataaa ttttgcgaaa atatcatttc
     2941 tgtcccactc ccaaaaagac agcagtagga taatttcaat ttggaaatat cttactgctg
     3001 ttttttatag taatagctat atttttgtat ttatgttttc tagtttaaca ttactactta
     3061 atgacttctt tatatttatc agcgaaattt gtaaagacac catcaacgcc atatttattt
     3121 aatcgtaaca tatcagcttt ttcattcact gtataaggat gtactataaa tcctaagtct
     3181 tttaaatgat gggtattttg ttcagttaaa tctgtataat caggacctaa tccaatcgca
     3241 taagagcgta tctcttttaa gcgttggtcg ttaaattgtt gtagttcacc tttatcaact
     3301 aattttacta atggcacatg cttattttga cgatgaattt tctttaaact ttcgtcagaa
     3361 aatgattgaa tcattacatg tccatttttt aatttattgt tatttaaaag gtgatgcttt
     3421 ttcaatgaag ctaataattg ttcttccatt cctgggtata catcaggtga ctttgtttca
     3481 atataatagt ttgcattcgg gccataacgt tctaaaattt catctaaagt gggtacttta
     3541 gcatttttat aacttgctct tgcgtatttt ggatattttt tattaaacca acttcctgca
     3601 tctaactgtt ttaattcatc aagggtataa tcctcaactt taccgtgtcc atttgttgta
     3661 cggttaacag tttcatcatg catagcaact aaatggccat ctttggtacg ttgtaaatca
     3721 atttcgatat aagatgcttt taactcatta tgactcttat catatgcttg aaacgtatgc
     3781 tcgggtgcat agccacttgc gccacgatgt gcgatagtag tgaatcgctc attcgttaaa
     3841 tttgtatgcc attgaatagc ctgaggttta tttgcaattt gatttgtttg ttcagcgcca
     3901 gcagtaggta ctgataaaaa tcccatagta aaaacagcag aagcagccat aaatttagtg
     3961 aagcttttcg aagagttagt catagtcttt atcctttcat ctctttattt gtagtctaac
     4021 aattttaaaa tactacaaaa acctgcttgt gaaaacgctt tacacatttc ttttacaatt
     4081 tataacattt ttgtaatata aatttaaaag aattatcaaa cattttagga atcttaaaaa
     4141 tgtgatgttc aacaagacga agagagcatt aaaataccca caatgtaatt ttaatagaca
     4201 ttgtgggtag gtagattaac gtatttattg tgatagtaat tgtttagcaa catcaagttg
     4261 ttgtttgacc gatgattgac ctgttgaacc gtaactttga cgtcgtttta aacaattttc
     4321 aggctgcaaa taatcgtaaa tatcggcatc aatactagaa tgatgttgtt gatatgttgc
     4381 taaaggaaca tctaataaat aatgaccttg ttgtatacat tctaagacga tttttcctac
     4441 aatttcatgt gcagttctaa atggaatatt tttagttact aaataatctg ctagttccgt
     4501 tgcatttgaa aaatcttctt taacagtttg attgagtcgt tctttattaa ttgtcatcgt
     4561 ttgaatcata ccttcgaaaa tacgtaaaga acctttaatt gtatggacag catcgaataa
     4621 accttcttta tcttcctgca tatctttgtt atatgctaga ggtaatcctt ttaaagtcat
     4681 aagcatgctc attaaatgac cagtcgttcg accaacttta cctctaatta attctgccat
     4741 atcaggattt ttcttttgtg gcataataga tgagccagtt gaaaatgcat ctgataatgt
     4801 aatgaattta gcttcgtctg tggaccagaa aataatttcc tctgcaaagc gtgataagtg
     4861 aaccatcgtt aaagaaatat tatgcaatgt ttcaataata tagtctctgt cactaacagc
     4921 atctaggcta ttctcataga gactgccaaa gttcaacaat gctgttgtct cgtgtctatc
     4981 gatagggtat gtggtaccac ttaaggctgc tgcacctaaa ggattaatat cgattcgttt
     5041 taaactatct tcaaatcgtt gttggtctcg ttgtaacatc caaaaataag tcataatatg
     5101 atgtgcaaat gaaattggct gtgcacgctg taaatgagta taactaggca taattgtatc
     5161 aacattattg gaagcgatgt ctacaattac actttgtaac gacttaatta atgcgatgat
     5221 atcttgcact tgtttcttag tgtacaagtg catgtctgtt gcaacttgat cgtttctact
     5281 gcgtccagta tgcaacttac caccagcatc accgatacgt ttaattaatt catgttcaat
     5341 atttaaatga atatcttcta atgatgcact aaattgaatt tgatcttgat gataatcatg
     5401 ttgaatagat tttagtcctt gtataatttg ttcgctgtct tgttgactaa taatgccttg
     5461 attcgcaagc atagttgcat gtgcaatgct gccttcgata tcttgatcta tgagcgtttg
     5521 atcaaaagta atggatgcgt taaagtcgtc aacccactct tcaggttgta cttcaaatct
     5581 accgccccaa gctttattgc tcattgctat agcctccatg caacatcgca tttacttgag
     5641 taggtaaacc atagatatcg ataaagccaa cagcagcgtc ttgattaaat gcatcttctt
     5701 ttgtataagt tgctaatttt tcatcatata atgtgtaagg tgattttcta ccattcacga
     5761 tggcattacc tttgaataat ttaattctga catcaccact tacgtattgc tgagtactat
     5821 caataaataa tttcaagcta tcagttaaag gtgagaacca aagtccattg tatagttgtt
     5881 cagcaaattg cttctcaatg attggtttaa agtgtgcgac atctttcgtt aacgtaatcg
     5941 tttctaatgc tttatgcgct tttaaaataa cttctgcagc aggtgcctca taaatttctc
     6001 ttgatttgat acctacaagt ctattttcta catggtcaat tcttccgata ccatgcttac
     6061 cagctaatgc attcaacgtt aaaattaaat cgtctaattc atatgttttg ccatcaattt
     6121 gaactgggat gcctttatca aacgttaaaa tgatttcatc agcagtatct ggtgtttctt
     6181 ctaaagcatt tgttagatcg aacgcatcct ctggtggcgc agcataagga tcttctaaaa
     6241 taccacattc attcgctctg ccccatagat tttgatcgat agaataaggt gaatcatggt
     6301 tgattgatac agggatatta tgtttaattg cataatcgat ttcttcttca cgactccatg
     6361 cccactcacg tacaggtgcg aatgctttca atgatgggtt taatgcttta atggcaactt
     6421 cgaaacgtac ttggtcattc cctttaccag tacaaccatg tgcaatacct actgaatttg
     6481 ttttctcagc aatctctact aattttttag cgattaatgg tcttgataaa gctgaaacta
     6541 atggatatgc attttcatac attaaatttc ctttgattgc ataacttaca tactcatcac
     6601 taaattcttt tgttgcatca ataatatgac attcaactgc tcccatatct aaagcttttt
     6661 tataaacgat gtctaaatct ttaccttcac caacatctag gcaacaagct acaacgtcgt
     6721 atcctttgtc gataagccat tgaacggcca cacttgtatc tagtcctcct gaatatgcta
     6781 aaacaatttt ctctttcata aaattcacct cattgtagtt atctgaatat ttcgtatgaa
     6841 cacatgatag caaactttta atgaaaataa aaccattatt ttacatattt attcataaaa
     6901 tgcgatagag atagttagag ttgtaagtgt aaatgcttat ttatcaatgt ttaaaagtat
     6961 tttaaaaaac atgcaaattg tataaaaaat gtttgagtgt ttgacgccag tttgaaatgt
     7021 ttgttgaaaa ataggtgtga aacgtcacat aaatatcaat tttattgatt aagtacaaac
     7081 ttgtgaattg aatcgcaaac gtaaacttag taaaattaag atattaaatg aaacaggagg
     7141 cttttataga tgactcatat tcaattagat tttagtaaaa cgttagaatt tttcggtgaa
     7201 cacgaattaa aacaacaaca agaaattgtt aaatcaattc acaaaacaat tcatgaaggt
     7261 actggtgcag gtagtgactt cttaggctgg gttgatttac cagttgatta cgacaaagaa
     7321 gaattttcaa gaattgttga agcatcaaaa cgcattaaag aaaattctga tgttttagta
     7381 gtcatcggta ttggtggttc ttacttaggt gcacgtgcag caatcgaaat gttaacgtca
     7441 tcatttagaa acagcaatga ataccctgaa attgtatttg ttggtaatca cttatcatca
     7501 acatatacga aagagttagt tgattattta gcagacaaag atttctctgt aaacgttatt
     7561 tctaaatctg gtacaactac agaaccagca gttgcattta gattgttcaa acaattagtt
     7621 gaagaaagat acggtaaaga agaagcacaa aaacgtatat ttgcaacaac ggataaagaa
     7681 aaaggtgctt taaaacagtt ggctacaaac gaaggttatg aaacgtttat cgtacctgat
     7741 gatgtaggtg gaagatattc tgttttaaca gcagtaggat tattaccaat tgcaacagct
     7801 ggaattaaca tcgaagctat gatgattggt gctgcaaaag cacgtgaaga attatcttca
     7861 gataaattag aagaaaacat tgcataccaa tatgcgacaa ttcgaaacat tttatatgca
     7921 aaaggttata caacagaaat gttgattaac tatgaaccat ctatgcaata ctttaatgaa
     7981 tggtggaaac aattatttgg tgaatcagaa ggtaaagact tcaaaggtat ctatccttca
     8041 agtgccaact acacaactga tttacattct ttaggtcaat atgtacaaga aggccgtcgc
     8101 ttcttattcg aaacagttgt aaaagtaaat catcctaaat atgatattac tattgaaaaa
     8161 gatagtgatg atctagacgg attaaattat ttagctggta aaacaatcga cgaagttaac
     8221 acaaaagcat tcgaaggtac attattagcg catactgatg gtggtgttcc taacatggta
     8281 gtgaacattc cacaattaga tgaagaaact ttcggttacg tcgtatactt cttcgaactt
     8341 gcttgtgcaa tgagtggata ccaattaggc gtaaatccat ttaaccaacc tggtgtagaa
     8401 gcatataaac aaaacatgtt cgcattatta ggtaaacctg gttttgaaga cttgaaaaaa
     8461 gaattagaag aacgtttata aaatacatta cttcaaagat tagtgaagtt tgaaaagata
     8521 gaactagacg ttaactattt aaagcatatt ttcgaggttg tcattacaaa tgtaaaaatg
     8581 taatgacaac ctcgttttta tttatatgca agaactaggt tactagctaa tgtgacaaga
     8641 tgttaagaga aaattaaaga taaaataata tctgccttac aataatattg ttatactact
     8701 agagactgat ttattagcat gattacatgt taatgtttct ttacttagta attaacttta
     8761 taatgtaaga ataattatct tcaaccaaag aaagggattg atgatttgtc gtttcatcaa
     8821 gtagaagaat ggtttgagat atttcgacag tttggttatt tacctggatt tatattgtta
     8881 tatattagag ctataattcc agtatttcct ttagcactct atattttaat taacattcaa
     8941 gcttatggac ctattttagg tatattgatt agttggcttg gattaatttc tggaacattt
     9001 acagtctatt tgatctgtaa acgattggtg aacactgaga ggatgcagcg aattaaacaa
     9061 cgtactgctg ttcaacgctt gattagtttt attgatcgcc aaggattaat cccattgttt
     9121 attttacttt gttttccttt tacgccaaat acattaataa attttgtagc gagtctatct
     9181 catattagac ctaaatatta tttcattgtt ttggcatcat caaagttagt ttcaacaatt
     9241 attttaggtt atttaggtaa ggaaattact acaattttaa cgcatccttt aagagggata
     9301 ttaatgttag ttgtgttggt tgtattttgg attgttggaa aaaagttaga acagcatttt
     9361 atgggatcga aaaaggagtg acatcgtgaa aaaagttgta aaatatttga tttcattgat
     9421 acttgctatt atcattgtac tgttcgtaca aacttttgta atagttggtc atgtcattcc
     9481 gaataatgat atgtcaccaa cccttaacaa aggggatcgt gttattgtaa ataaaattaa
     9541 agttacattt aatcaattga ataatggtga tatcattaca tataggcgtg gtaacgagat
     9601 atatactagt cgaattattg ccaaacctgg tcaatcaatg gcgtttcgtc agggacaatt
     9661 ataccgtgat gaccgaccgg ttgacgcatc ttatgccaag aacagaaaaa ttaaagattt
     9721 tagtttgcgc aattttaaag aattagatgg tgatattatt ccgccaaaca attttgttgt
     9781 gctaaatgat caagataata acaagcacga ttcaagacaa tttggtttaa tcgataaaaa
     9841 ggatattatt ggtaatgtta gtttacgata ctatcctttt tcaaaatgga ctgttcagtt
     9901 caaatcttaa aaagaggtgt caaaattgaa aaaagaaata ttggaatgga ttatttcaat
     9961 tgcagtcgct tttgtcattt tatttatagt aggtaaattt attgttacgc catatacaat
    10021 taaaggtgaa tcaatggatc caactttgaa agatggcgag cgagtagctg taaacattgt
    10081 tggatataaa acaggtggtt tggaaaaagg taatgtagtt gtcttccatg caaacaaaaa
    10141 tgatgactat gttaaacgtg tcatcggtgt tcctggtgat aaagtagaat acaaaaatga
    10201 tacattatat gtcaatggta aaaaacaaga tgaaccatat ttaaactaca atttaaaaca
    10261 taaacaaggt gattacatta ctgggacttt ccaagttaaa gatttaccga atgcgaatcc
    10321 taaatcaaat gtcattccaa aaggtaaata tttagtgctt ggagataatc gtgaagtaag
    10381 taaagatagc cgtgcgtttg gcctcattga tgaagaccaa attgttggta aagtttcatt
    10441 taggttctgg ccatttagtg aatttaaaca taatttcaat cctgaaaata ctaaaaatta
    10501 atatgaaaca aatacaacat cgtttgtcgg ttttaatact gataaacgat gttttatttt
    10561 gttagtacca caataaaagc taagttcgaa atgaacttat aataaatcaa tcacaatcac
    10621 tttgtgttaa aatatgtgtc aaaggaagtg agggtttgtc atgacattac atgcttattt
    10681 aggtagagcg ggaacaggta agtctacgaa aatgttgacc gaaataaaac aaaaaatgaa
    10741 agcagatccg cttggagatc caatcatttt aattgcgcca actcaaagta catttcaatt
    10801 agaacaagcc tttgtcaatg atccggaatt aaatggtagt ttaagaacag aagtgttgca
    10861 ttttgaacga ttaagtcatc gtattttcca agaagttggt agttatagcg aacaaaagtt
    10921 atctaaagct gcaacggaaa tgatgattta taacattgtt caagaacaac aaaagtattt
    10981 aaaactttat caatcacaag caaaatatta tgggtttagt gaaaaattaa cagaacaaat
    11041 tcaagatttt aaaaaatatg cagtaacgcc tgaacattta gaacacttta ttgctgataa
    11101 aaatatgcaa actcgaacta aaaataagtt agaggatatt gctttaatat accgtgagtt
    11161 cgaacaacgc attcaaaacg agtttattac gggtgaggat tcattacaat attttattga
    11221 ttgtatgccg aaatcagagt ggctaaaacg tgctgatata tatattgatg gttttcacaa
    11281 cttttcaacg attgagtatt taataatcaa aggattaatt aaatatgcga agagtgtcac
    11341 aattatattg acgacagatg gtaaccacga tcaatttagt ttatttagaa aaccatcgga
    11401 agtgttacga catattgaag aaatagcaaa tgaactcaat atttctattg aacgtcaata
    11461 tttcaaccaa ttatatcgct tcaataatca agatttaaag catcttgaac aagaatttga
    11521 tgcacttcaa atcaatcgag tggcatgtca aggtcatatc aatattttag aatctgcgac
    11581 tatgagagag gaaataaatg aaattgcgcg acgtatcatc gttgatattc gtgataagca
    11641 attacgatat caagatattg ctattttata tcgtgatgaa tcttatgctt atttatttga
    11701 ttccatatta ccgctttata atattcctta taatattgat acaaagcgtt cgatgacaca
    11761 tcatccggtc atggaaatga ttcgttcatt gattgaagtt attcaatcta attggcaagt
    11821 gaatccaatg ctacgcttat tgaagactga tgtgttaacg gcatcatatc taaaaagtgc
    11881 atacttagtt gatttacttg aaaattttgt acttgaacgt ggtatatacg gtaaacgttg
    11941 gttagatgat gagctattta atgtcgaaca ttttagcaaa atggggcgta aagcgcataa
    12001 actgaccgaa gatgaacgta acacatttga acaagtcgtt aagttaaaga aagatgtcat
    12061 tgataaaatt ttacattttg aaaagcaaat gtcacaagcg gaaactgtaa aagattttgc
    12121 aactgctttt tatgaaagta tggaatattt cgaactgcca aatcaattga tgacagagcg
    12181 agatgaactt gatttaaatg gtaatcatga aaaggcggag gaaattgatc aaatatggaa
    12241 tggcttaatt caaatccttg atgacttagt tctagtattt ggagatgaac caatgtcgat
    12301 ggaacgtttc ttagaagtat ttgatattgg tttagaacaa ttagaatttg ttatgattcc
    12361 gcaaacattg gaccaagtaa gtattggtac gatggatttg gctaaagtcg ataataagca
    12421 acatgtttac ttagtaggta tgaatgatgg aacgatgcca caaccagtaa ctgcgtcaag
    12481 cttgattaca gatgaagaaa agaaatactt tgaacagcag gctaatgtcg agttaagtcc
    12541 aacatcagat attttacaga tggatgaagc atttgtttgt tatgttgcta tgactagagc
    12601 taagggagat gttacatttt cttacagtct aatgggatca agtggtgatg ataaggagat
    12661 cagcccattt ttaaatcaaa ttcaatcatt gttcaaccaa ttggaaatta ctaacattcc
    12721 tcaataccat gaagttaacc cattgtcact aatgcaacat gctaagcaaa ccaaaattac
    12781 attatttgaa gcattgcgtg cttggttata tgatgaaatt gtggctgata gttggttaga
    12841 tgcttatcaa gtaattagag atagcgatca tttaaatcaa ggtttagatt atttaatgtc
    12901 agcattaacg tttgacaatg aaactgtaaa attaggtgaa acgttgtcta aagatttata
    12961 tggtaaggaa atcaatgcca gtgtatcccg ttttgaaggt tatcaacaat gcccatttaa
    13021 acactatgcg tcacatggtc tgaaactaaa tgagcgaacg aagtatgaac ttcaaaactt
    13081 tgatttaggt gatattttcc attctgtttt aaaatatata tctgaacgta ttaatggcga
    13141 ttttaaacaa ttagacctga aaaaaataag acaattaacg aatgaagcat tggaagaaat
    13201 tttacctaaa gttcagttta atttattaaa ttcttcagct tactatcgtt atttatcaag
    13261 acgcattggc gctattgtag aaacaacact aagcgcatta aaatatcaag gcacgtattc
    13321 aaagtttatg ccaaaacatt ttgagacaag ttttagaagg aaaccaagaa caaatgacga
    13381 attaattgca caaacattaa cgacaactca aggtattcca attaatatta gagggcaaat
    13441 tgaccgtatc gatacgtata caaagaatga tacaagtttt gttaatatca ttgactataa
    13501 atcctctgaa ggtagtgcga cacttgattt aacgaaagta tattatggta tgcaaatgca
    13561 aatgatgaca tacatggata tcgttttaca aaataaacaa cgccttggat taacagatat
    13621 tgtgaaacca ggtggattat tatacttcca tgtacatgaa cctagaatta aatttaaatc
    13681 atggtctgat attgatgaag ataaactaga acaagattta attaaaaagt ttaagttgag
    13741 tggtttagtt aatgcagacc aaactgttat tgatgcattg gatattcgtt tagaacctaa
    13801 attcacttca gatattgtac cagttggttt gaataaagat ggctctttga gtaaacgagg
    13861 cagccaagtg gcagatgaag caacgattta taaattcatc caacataaca aagagaattt
    13921 tatagaaaca gcttcaaata ttatggatgg acatactgaa gttgcaccat taaagtacaa
    13981 acaaaaattg ccatgtgctt tttgtagtta tcaatcggta tgtcatgtag atggcatgat
    14041 tgatagtaag cgatatcgaa ctgtagatga aacaataaat ccaattgaag caattcaaaa
    14101 tattaacatt aatgatgaat ttgggggtga gcaatagatg acaattccag agaaaccaca
    14161 aggcgtgatt tggactgacg cgcaatggca aagtatttac gcaactggac aagatgtact
    14221 tgttgcagcc gcggcaggtt caggtaaaac agctgtacta gttgagcgta ttatccaaaa
    14281 gattttacgt gatggcattg atgtcgatcg acttttagtc gtaacgttta caaacttaag
    14341 cgcacgtgaa atgaagcatc gtgtagacca acgtattcaa gaggcatcga ttgctgatcc
    14401 tgcaaatgca cacttgaaaa accaacgcat caaaattcat caagcacaaa tatctacact
    14461 ccatagtttt tgcttgaaat taattcaaca gcattatgat gtattaaata ttgacccgaa
    14521 ctttagaaca agcagtgaag ctgaaaatat tttattatta gaacaaacga tagatgaggt
    14581 catagaacaa cattacgata tccttgatcc tgcttttatt gaattaacag agcaattgtc
    14641 ttcagataga agtgatgatc agtttcgaat gattattaaa caattgtatt tctttagcgt
    14701 tgcaaatcca aatcctaaaa attggttgga tcaattggtg acaccatacg aagaagaagc
    14761 acaacaagcg caacttattc aactactaac agacttatct aaagtattta tcacagctgc
    14821 ttatgatgct ttaaataagg cgtatgattt gtttagtatg atggatagcg tcgataaaca
    14881 tttagctgtt atagaagatg aacgacgttt aatggggcgt gttttagaag gtggctttat
    14941 tgatatacct tatttaactg gtcacgaatt tggcgcgcgt ttgcctaatg taacagcgaa
    15001 aattaaagaa gcaaatgaaa tgatggtcga tgcctt
//
LOCUS       DAKTGS010000064        14976 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.064, whole genome shotgun sequence.
ACCESSION   DAKTGS010000064 DAKTGS010000000
VERSION     DAKTGS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.064"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            107..940
                     /locus_tag="O0286_001579"
     CDS             107..940
                     /locus_tag="O0286_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCY0169959.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            1222..2019
                     /locus_tag="O0286_001580"
     CDS             1222..2019
                     /locus_tag="O0286_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0169960.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            2055..3059
                     /locus_tag="O0286_001581"
     CDS             2055..3059
                     /locus_tag="O0286_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0169961.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            3056..4072
                     /locus_tag="O0286_001582"
     CDS             3056..4072
                     /locus_tag="O0286_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0169962.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            4306..5274
                     /gene="dhaK"
                     /locus_tag="O0286_001583"
     CDS             4306..5274
                     /gene="dhaK"
                     /locus_tag="O0286_001583"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCY0169963.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            5316..5900
                     /gene="dhaL"
                     /locus_tag="O0286_001584"
     CDS             5316..5900
                     /gene="dhaL"
                     /locus_tag="O0286_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCY0169964.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            5893..6255
                     /locus_tag="O0286_001585"
     CDS             5893..6255
                     /locus_tag="O0286_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCY0169965.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            6371..6868
                     /locus_tag="O0286_001586"
     CDS             6371..6868
                     /locus_tag="O0286_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169966.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            7324..8391
                     /locus_tag="O0286_001587"
     CDS             7324..8391
                     /locus_tag="O0286_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169967.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            8542..9585
                     /locus_tag="O0286_001588"
     CDS             8542..9585
                     /locus_tag="O0286_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0169968.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            9914..10342
                     /locus_tag="O0286_001589"
     CDS             9914..10342
                     /locus_tag="O0286_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0169969.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(10551..11057)
                     /locus_tag="O0286_001590"
     CDS             complement(10551..11057)
                     /locus_tag="O0286_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0169970.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            11170..12093
                     /gene="graX"
                     /locus_tag="O0286_001591"
                     /gene_synonym="apsX"
     CDS             11170..12093
                     /gene="graX"
                     /locus_tag="O0286_001591"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCY0169971.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            12109..12783
                     /gene="graR"
                     /locus_tag="O0286_001592"
                     /gene_synonym="apsR"
     CDS             12109..12783
                     /gene="graR"
                     /locus_tag="O0286_001592"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCY0169972.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            12776..13816
                     /gene="graS"
                     /locus_tag="O0286_001593"
                     /gene_synonym="apsS"
     CDS             12776..13816
                     /gene="graS"
                     /locus_tag="O0286_001593"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCY0169973.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            13960..14721
                     /gene="vraF"
                     /locus_tag="O0286_001594"
     CDS             13960..14721
                     /gene="vraF"
                     /locus_tag="O0286_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCY0169974.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            14711..>14976
                     /locus_tag="O0286_001595"
     CDS             14711..>14976
                     /locus_tag="O0286_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0169975.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELA"
BASE COUNT         5250 a         2018 c         2793 g         4915 t
ORIGIN      
        1 agttaaatag taaatctttg ttagaactct ttccatagag ttgtgacagc atttttgttg
       61 tgctactatt tttatagtct aataaatata taaaggggat ggtttcgtga ataaaacggt
      121 taaagattta atactagttg tcttaggttc atttatcttt gctgcaggtg taaatgcatt
      181 tattatttct ggtaacttag gtgaaggcgg ggttacaggt ttagcaatta ttttatatta
      241 tgcgtttcat atttcaccag ccatcactaa cttcttggtc aacgcagtat tgattgccat
      301 aggttataaa tttttgagta agagaagtat gtacttaact attcttgtaa caattcttat
      361 ttcaatattt ttgagtttaa cagaatcatg gcaagtagaa actggaaaca gcattgtgaa
      421 tgccattttt ggtggtgtaa gcgttggact aggaatcgga gtaattatcc ttgcaggcgg
      481 tacaacagca ggtacaacaa ttttggcgag aattgcaacg aaatacctcg atgtaagcac
      541 gccatatgct ttgcttttct tcgatatgat cgttgttgca atttcactta cagttattcc
      601 acttgataaa gtattagtaa cagtaatatc actttatata ggaacaaaag tgatggaata
      661 tgtcatagaa ggtttaaaca ctaaaaaagc tatgacgatt atttcaacta atcccgacaa
      721 acttgccaaa gcaatagacg agcaaattgg aagaggttta accattttaa acggacatgg
      781 ctattatacg cgtgaagaaa aagatgtctt atacgttgtt atttctaaaa cacaagtttc
      841 aaaagcaaag cgattaatta aacaaatcga taaagatgca ttcctcgtaa ttcatgatgt
      901 aagagatgtc tatggtaatg gctttcttgc agatgaataa ataaatggta tgagcacaca
      961 tacttaaata gaagtccacg gacaagtttt tgaactatga agacttatct gtgggcgttt
     1021 tttattttat aaaagtaata tacaagacat gacaaatcga gctatccaat ttaaaaagta
     1081 atgttagtca ataagattga aaaatgttat aatgatgttc atgataatca ttatcaattg
     1141 ggatgtcttt gaaaattgat aatttaaaaa tagaaattat tttttataaa cagaaagaat
     1201 tttattgaaa gtagggaaat tatgaatcgt ttgcatggac aacaagttaa aattggttac
     1261 ggggataaca cgattataaa taaattagat gttgaaatac cagatggcaa agtgacgtca
     1321 atcattggtc ctaacggctg cgggaaatct actttgctaa aggcattgtc acgtttattg
     1381 gcagttaaag aaggcgaagt atttttagat ggtgaaaata ttcatacaca atctacgaaa
     1441 gagattgcaa aaaaaatagc cattttacct caatcacctg aagtagcaga tggcttaact
     1501 gttggggaat tagtttcata tggtcgtttt ccacatcaaa aaggatttgg tagattaact
     1561 gctgaggata agaaagaaat tgattgggca atggaagtta caggaactga tacattccga
     1621 caccgttcaa tcaatgattt aagtggtggt caaagacaac gtgtttggat tgcaatggca
     1681 ttagcacaaa gaactgatat tatcttttta gacgaaccaa caacatattt agatatctgt
     1741 catcaattag aaatactaga attagttcag aagctaaatc aggaacaagg ttgtacaatt
     1801 gtcatggttc ttcatgatat caaccaagcg attcgtttct cagatcatct tattgcgatg
     1861 aaagaagggg atatcatcgc tacaggttca acagaagacg tattaacaca ggaaatatta
     1921 gaaaaagttt ttaatattga tgttgtttta agtaaagatc ctaaaactgg aaaaccttta
     1981 ctggtaactt atgacttatg tcgcagagct tattcttaat taagtaagtt aatatgataa
     2041 aaaggacaat taacatgaca aatagagaga acccaacgcc attgaagttt ttatcctata
     2101 ttataggttt aagtatgata ctactaatca cactatttat ttctacatta ataggtgacg
     2161 ccaaaattca agcctctaca attatagagg ctatttttaa ttataatcct agcaatcaac
     2221 agcaaaacat catcaatgag attaggattc ccagaaatat agcagcagta attgtaggta
     2281 tggcgcttgc agtttctggt gcgattatac aaggtgttac tcgtaatggt cttgctgatc
     2341 cggcgctcat aggtttaaat tcaggtgctt catttgcttt agcattaaca tatgcagttt
     2401 taccaaacac ttcattttta atattgatgt ttgctggatt tttaggtgct attctaggag
     2461 gtgctattgt attaatgata ggccgatcta gacgtgatgg atttaatccg atgcgtatta
     2521 ttttagcggg tgcagcagta agtgctatgt taacagcgct aagtcaaggt attgcattag
     2581 cttttagact aaatcaaaca gtaacatttt ggactgctgg aggcgtttca ggcacaacat
     2641 ggtcacacct taagtgggca attccattaa ttggtattgc gttattcatt atattaacaa
     2701 ttagtaaaca acttaccatt ttaaatcttg gtgaatcatt agctaaaggt ttaggtcaaa
     2761 atgtaacaat gatcagaggc atatgtttaa ttattgctat gattctagca ggtattgcag
     2821 ttgctatcgc tggacaagtt gcatttgtag gtttgatggt acctcatata gcaagatttt
     2881 taattggaac tgattatgct aaaattctac cattaacagc cttgttaggt gggatactcg
     2941 tgcttgttgc cgatgtgata gcacgatatt taggagaagc gcctgttggt gcaatcattt
     3001 catttatcgg tgttccttac tttttatatt tagttaaaaa aggaggacgc tcaatatgat
     3061 tagttcaaat aataaacgca gacaattgat agcactggct gtttttagca ttctactatt
     3121 tctaggttgt acttggagta ttacctcagg tgaatacaac atacctgttg aaagattttt
     3181 caaaacttta attggacaag gtgatgccat tgataagtta atcttattag atttcaggtt
     3241 acctcggatg atgattacta ttttggctgg cgcagcgctt agtattagtg gtgcaatagt
     3301 gcaaagtgtc acaaaaaatc caatagctga accaggtata ttaggtatta acgcaggtgg
     3361 cggatttgca atcgcattat ttattgcaat tggtaaaatt aatgctgaca actttgttta
     3421 tgtactgccg ttaataagta tactaggtgg tatcaccact gcattgatta tttttatttt
     3481 cagttttaat aaaaatgaag gtgttacacc tgcgagtatg gtattaatag gtgtaggttt
     3541 acaaacagca ttatatggtg gctcaattac aattatgtca aaatttgatg ataagcaatc
     3601 tgatttcatc gctgcttggt ttgcaggtaa tatttggggt gacgaatggc catttgtcat
     3661 tgcattttta ccgtgggtgt tgattattat tccttactta ctatttaaat cgaatacact
     3721 aaatattatt catacgggtg ataatattgc acgaggtcta ggtgtaaggt taagcagaga
     3781 acgtttaata ttattcttta tcgcagtgat gttatcatct gctgctgtag cagtagcagg
     3841 ttcaatttcg tttatcggat taatgggtcc gcatattgcc aaacgtatcg ttggaccacg
     3901 tcaccagttg tttttaccaa ttgccatttt agtaggggca tgtttacttg ttatagctga
     3961 tacaattggc aaaattgtat tacaaccagg tggggttcca gcaggtattg tcgtagcaat
     4021 tattggtgca ccgtatttct tatatttaat gtacaaaacg aaaaatgtat agtgtcaatg
     4081 gacacaactt attgctatga aaggcacttt attataaggc ttttcatagc attttttatt
     4141 taatgagcca ctcaagacta tttatttttt caataatgaa ccattaagtt atcaagagga
     4201 tcttatcaaa aatatatttg ataacggtat caggttaatt ctttatgata gcgcattcat
     4261 ttattctgtt ttatactatg actgataata ccaaggaggt acaacatgat gaaaaagtta
     4321 atcaataaaa aagaaacatt tttaactgat atgcttgaag gattgttaat tgcgcaccca
     4381 gagttagatc tgattgctaa tacagttatt gtaaaaaaag ctaagaaaga acatggtgta
     4441 gcaatagtct ctggaggtgg aagcggacat gaacctgcgc atgccggttt tgttgcagaa
     4501 ggtatgctag atgcagcggt ttgtggcgaa gtatttacat cacctacacc tgataaaata
     4561 ttagaagcta ttaaagcagt agatactggt gatggtgtat tactagttgt aaaaaactat
     4621 gcaggtgacg tgatgaattt cgaaatggca caagagcttg cagaaatgga aggtataaat
     4681 gttcaaactg ttattgttcg tgacgacatt gctgtgacaa acgaagtaca acgtcgtggt
     4741 gttgcaggaa cagtgtttgt tcataagctt gccggttatc ttgctgaaaa aggttattca
     4801 ttaacagaga taaaatcgcg tgtagaagcg ttgttacctg aaattaaaag tattggtatg
     4861 gcaattgagc caccgcttgt tccaactact ggaaaatatg gctttgatat tgaagacgac
     4921 aaaatggaaa tcggtattgg tatacatggt gaaaaaggta ttcataggga agaagtaaag
     4981 gatattgatc atattgttgg aacattgtta gacgaattgt ataaagaagt tactgccaat
     5041 gatgtcatat taatggtaaa tggtatgggt ggtacgccgt tatctgaatt aaatatcgta
     5101 actaaatata ttcaacaaaa tttagctgca agaacggtta atgttgctaa atggtttgtt
     5161 ggtgattata tgacatcttt agacatgcaa ggtttttcta taactatcgt gcctaataaa
     5221 ccagaatatt tggaagcatt tttagcacca acaacaagtc aatactttaa ataagaagtg
     5281 aatatgaata aatatacatt tatgaggtgg cacaaatgaa agtgaatgat atgaaagcac
     5341 gtttattaaa tttagaagaa acgtttaaaa aacatgaatc tgaattaact gaattagatc
     5401 gagcaattgg tgatggtgac cacggggtta acatggttcg tgggtttagt agtcttaaag
     5461 acaaacttga tgatagctca atgcaatcat tgttcaaatc aactggtatg gcattgatgt
     5521 caaatgttgg gggtgcatca ggaccactgt atggctttag ctttgttaaa atgtctgcag
     5581 tcaccaaaga tgatatggat aatcaagatt tcattacact aattcaggca tttgccgaag
     5641 cggttgaatc acgtggtaaa gttactttaa atgaaaagac aatgtatgat gtagtagcgc
     5701 gagcagcaga gaagcttaaa aatggtgaaa ctttaacatt caatgattta cagcaattag
     5761 cagataatac aaaagatatg gtagcaacga aaggtagagc tgcatatttt ggagaagaat
     5821 caaaaggtta tattgatcca ggtgctcaaa gtatggttta tattttaaac gctttgattg
     5881 gagatgaaga taatgcctaa aattatactt gttagccaca gtaaagaaat tgcaagtggt
     5941 acaaaatctt tgttaaagca aatggcaggt gacgttgata ttataccaat cgggggatta
     6001 ccagatggtt caattggaac ttcatttgat atcatccaag aagttttgac taaattagag
     6061 gatgatgcat tgtgttttta cgatattgga tcttcagaaa tgaatgtaga tatggcaatt
     6121 gaaatgtatg atggtaatca tcgtgtgtta aaagttgatg caccaattgt tgaaggcagt
     6181 tttatcgcag cagtaaagct atcaatcggc ggttcaattg atgatgcatt agcagaaatc
     6241 aaacaatcat tttagttaaa atttactaat aatgaaaaat gtaaaccttt ttcaaatgaa
     6301 actttataaa aaatatgata gtatatatgt aaatgtttaa taaaatctgg agaaatagga
     6361 ggacattgcc atgcaacacc ttataaaaaa acatgtattg aatggcgagt ttgatttagt
     6421 acgacaattg atgtccgaaa cagattttat ggaatttgaa gaagcatata tttcaagtgc
     6481 gcatgaagta gaaagtatga tgttttatac atgtatttta gatatgatta agtacgaaga
     6541 atcatctgaa atgcatgact tagcattttt attgcttgtg tatccactaa gtgaatatga
     6601 aggtgctttg gattctgctt attatcatgc agacgcttcc ataaaactta ctgacggcaa
     6661 agaagttaaa agtttgttac aaatgttatt attgcatgcg ataccaacac ctgttatttc
     6721 agataagaag gcttttgata tcgccaagca aattttaaaa ttagatccta ataataatgt
     6781 tgctcgtaac gtcttaaaag acactgccaa acgtatggac aacgttgttg ttgatataaa
     6841 tgaattacac caacgtaatg cacgttaatt acatttcaat tatattagct taataatagt
     6901 tttaacattt ggttgggttg ggcatatgtt ccagcctttt ttaatactta aaaactaacg
     6961 aagtatactt gtgtgcacaa atggttttta tacaacattt tataaattta tacattttaa
     7021 taaagaacat acgatagatg gtttaaacct tgttaactga gaaattttga tatgtattct
     7081 tcgaaattta actaaatata cgaaattcaa gaagcacaat aattaatcat ttttcctata
     7141 caaaagttcg tatgactgca ttataaaagc ataaatttat aattttttta aatgtcattg
     7201 aacgtgataa tgtgaatgga ttgagcaatt ttgaaaaagt gaaaaataac ctatgcggct
     7261 tgcaattaat tttcagtacg ttataatgca cactgtgcaa aattaaggag gtctattatt
     7321 cacatgatga tgaataaaga agcaacaaaa attggatttg cctacgtcgg cattgtagtg
     7381 ggcgcaggat tttcaactgg acaagaagtt atgcaatttt tcactaaata tggcttgtgg
     7441 gcttatttag gtgttattat atctggtttt attttagctt ttattgggcg ccaagtagca
     7501 aaaattggta ctgcctttga agcgacaaat catgaatcaa cattacaata cgtattcggt
     7561 gaaaagttta gtaaagtctt tgattatatt ttaatcttct tcttatttgg tatagctgta
     7621 accatgatag ctggtgcagg cgcaacattt gaagaaagtt ataacatacc tacatggcta
     7681 ggtgctttaa ttatgacatt agcgatttat attacgttgc tattagactt taataaaata
     7741 gtacgtgcac taggtatcgt tacaccattt ttaattgttt tagttgtatt aatcgctggc
     7801 gtttatttat ttaaaggtca tgtttcatta gcagaagtta accaagtagt gcctgaagca
     7861 agtatttgga agggaatctg gtttggtaca atatatggtg gattagcttt ttctgtaggt
     7921 tttagtacca tcgtagcaat cggtggggat actgaaaagc gtacagtgtc aggtgcaggc
     7981 gcgatgtatg gtggtattat ctatactgta ttactagcat tgatcaactt tgcattgcaa
     8041 agtgaatatc caactattaa aaatgcctca attcctacat tgacgttagc aaataatatc
     8101 catcctttaa tagcaacagt gttatctgtt attatgctgg cggttatgta taatactatt
     8161 ctaggactaa tgtattcatt tgcagcacgt tttacagaac catacagtaa aaattatcat
     8221 atctttatta ttataatgat ggtagcaggt tatttattaa gtttcgtagg atttgctgaa
     8281 ttaattaata agttatatac aattatggga tatgtaggct tatttattgt agtagctgta
     8341 attattaaat atttcaaacg taaaaatgcg gataaaaaac atattgctta atatcatatg
     8401 agggatatcc gaaactttac aattgaatca ctttgtttta accttaaaag caattcgtct
     8461 ctactcttat cgggcgaatt gctttttata tttattcagt ctattaatat gagcgtctaa
     8521 caaatagaga ggtacgatgt aatgaataaa gataataaat ggacgatgat aactgcgctt
     8581 tttataactg taatcagtgt attgttagca tttcatctga aacaacatta tgaccaaatt
     8641 acaaatgaga accatgctaa taaagacaaa attaatatta aaaataaaaa tgtgcgcatt
     8701 tatcaaaacc ttacatacaa tagagttttc cctaacagta aattagatat tattacacct
     8761 gttgatatgt cttctaatgc caaactgcca gttatttttt ggatgcacgg tggtggttat
     8821 attgcgggtg ataagcagta taaaaaccca ttattagcga aaattgctga acaagggtac
     8881 attgttgtga atgtaaatta tgcattggcg ccacaatata aatatcccac accattaatt
     8941 caaatgaatc aagcaactca attcattaaa gaaaataaaa tgaatttacc tattgatttt
     9001 aatcaagtaa ttattggcgg tgattctgca ggtgctcaat tagctagcca atttacggca
     9061 atacagacga atgatcgctt aagagaagcc atgaaatttg atcagtcatt caaaccatcg
     9121 caaattaaag gtgctatact atttggtggt ttttataata tgcaaacagt tagagaaact
     9181 gagtttccaa gaatacagtt atttatgaaa agttatactg gcgaagaaga ttgggaaaag
     9241 agttttaaaa acatttcaca aatgtcgaca gtaaaacaat cgacaaaaaa ttatccacca
     9301 acatttttat ctgttggaga tagcgatcca ttcgaaagtc aaaatataga attcagtaag
     9361 aaattacaag aattgaatgt accagtagat actttgtttt atgatggtac gcatcattta
     9421 catcatcagt atcaatttca ccttaataaa cctgaatcga tagataatat caaaaaagtg
     9481 ttacttttct taagtcgtaa tacatcctct agtggtattc aaactgaaga gaaaccacaa
     9541 atagaaaatc cgagtaatga attaccgtta aatcctttaa actaatgata aacagtagta
     9601 atttattgct taagcaacat ttaagatttt caaattaaaa acgaagaatt taaaacatgt
     9661 ggtgctaatg tgtaagagtc tgagatataa taaattgtta gcggttcttt atcatttcta
     9721 tctcactcta ttatgatgtg acatttattt tacaaactaa tttgttttga acctgaaaat
     9781 aactttttac aactaaattg tcgaaaaaca gtgtgtacat gatataataa atttataaat
     9841 tgaaaagaat tcaaaagaaa aattaaaata tagattgagc acgaagtgat ttgaaataag
     9901 gttgtgaaag ggaatgacaa ggtcagcatt aaaaccattt aaaaataaac gcgttatggt
     9961 tactggacgt atacaacgtg ttttgtttaa aaattattta gatagacata gcacatttaa
    10021 gccgaatgta aggatattat taaaagatgt atttgtttca ggtgtatcaa tagatcattt
    10081 atggttatat gagacaaata aatactatgc attggcaatg gaacttattc atcaacgagt
    10141 aaaatttagt gcgaatgttg taccgtatta caaaataaat agaaataata atttattcgt
    10201 acaagattat ggaattaagc gtaaaggtag gttaattact gaagaagctt acaatcaaaa
    10261 caatcagtat caggataaga tatatgaaaa attaccggat atagatttta gactcgaaga
    10321 tttttatagt aaggaaaact aaacaacata tataaagcac cactaatcgt agttgatagt
    10381 taatcaatta cacattagtg gtgttttatt ttgaatttaa atttgataaa tgaataagag
    10441 tgtacgtatt tatttgagat tagataacat tgataagtat gagtaatgta ttaggtaacc
    10501 attaaattcg ttgttatacg attctgatac aagattatga taaaatagct ttaattaaga
    10561 attttagcca tcatatagtc atcataatat ttaccatcga taaataactt atcttttaaa
    10621 acgccttcga tttgaaaatc ggcactttta aaaagctcga gggcaggttg gttattgagt
    10681 ggtacatttg cttcaattcg gtgtatttga ttgtttaaac accaagccat aatggcatca
    10741 agaagtgctt ggccaattcc acgatgttga tataatttct ttacacctaa atcaatttta
    10801 gcaacatgtt taatgcgttg aaatggtgtc gtattaacaa aggcaaagcc aacgagttgt
    10861 tcatcacttt cagcaacgaa gatgacttta tgcggagaag tgatatattc ttctaattgt
    10921 ttactagccg atgtgacgct aggatcatat tctcctggtg tgtagaacat atacggagat
    10981 tcgtcgtata tgttcgctaa cattgaaatg aaattttcta catctttgat actaactcta
    11041 cgtataatat gggccataaa aagcctccag tattttgaaa ttttaaaaca tttgctacta
    11101 ttataatata tatgtaacta aaaggtggag taatatgttt tttggttaca gtttaaagga
    11161 gtattttaga tgaaacctaa agttttatta gcaggtggaa caggatatat tggtaagtat
    11221 ttaagtgaag tgattgaaaa tgatgctgaa ctttttgcta tatcaaaata tccagacaat
    11281 aaaaaaacag atgatgttga aatgacttgg attcagtgtg atatatttca ttacgaacag
    11341 gttgttgcag caatgaatca aatagatatt gctgtattct ttatcgaccc aacaaagaat
    11401 tctgccaaaa taacacaatc atcagcaaga gatttaacat taatcgcagc agataatttt
    11461 ggtcgagcag cggctatcaa tcaagtaaaa aaagtaatct acatacctgg gagtcgttat
    11521 gataatgaaa caattgaacg cctaggtgca tatggtacac ctgtagaaac aacaaattta
    11581 gtttttaaac gttctttagt taatgtagaa ttacaagttt caaagtatga tgatgttaga
    11641 tcaacgatga aggtagtttt accaaaggga tggacattaa agaacgttgt aaaccatttt
    11701 attgcatgga tgggttacac taaaggaact tttgtgaaaa cagaaaaatc acatgatcaa
    11761 tttaagatat atattaagaa taaggtgcga ccgctcgcag tatttaaaat agaagaaaca
    11821 gctgatggaa taataacttt aattttattg agtggaagtt tagtgaaaaa atatacagtt
    11881 aatcaaggga agttagaatt tagattaatc aaagagtcgg cagtcgttta tatacatcta
    11941 tacgattata tccctcgatt attttggccg atttattact ttatacaagc accaatgcaa
    12001 aaaatgatga ttcatggctt tgaagttgac tgccggatta aagattttca aagtcgatta
    12061 aaatcaggag aaaatatgaa atatactaaa tgatattggg tgatatggat gcaaatacta
    12121 ctagtagaag atgacaatac tttgtttcaa gaattgaaaa aagaattaga acaatgggat
    12181 tttaatgttg ctggtattga agatttcggc aaagtaatgg atacatttga aagttttaat
    12241 cctgaaattg ttatattgga tgttcaatta cctaaatatg atgggtttta ttggtgcaga
    12301 aaaatgagag aagtttccaa cgtaccaata ttatttttat catctcgtga taatccaatg
    12361 gatcaagtga tgagtatgga acttggcgca gatgattata tgcaaaaacc gttctatacc
    12421 aatgtattaa ttgctaaatt acaagcgatt tatcgtcgtg tctatgagtt tacagctgaa
    12481 gaaaaacgta cattgacttg gcaagatgct gtcgttgatc tatcaaaaga tagtatacaa
    12541 aaaggtgatc agacgatttt cctgtccaaa acagaaatga ttatattaga aattcttatt
    12601 accaaaaaaa atcaaatcgt ttcgagagat acaattatca ctgcattatg ggatgatgaa
    12661 gcatttgtta gtgataatac gttaacagta aatgtgaatc gtttacgaaa aaaattatct
    12721 gaaattagta tggatagtgc aatcgaaaca aaagtaggaa aaggatatat ggctcatgaa
    12781 taatttgaaa tgggtagctt attttttgaa atctcgcatg aactggatat tttggatatt
    12841 gtttttaaac ttccttatgt taggcattag tctaatcgat tatgattttc caatagacag
    12901 tttattttat attgtttctt tgaatttaag tttaacaatg atttttcttt tattgacata
    12961 ttttaaagaa gtaaaattat ataagcattt tgacaaagat aaagaaatag aagaaattaa
    13021 acataaagat ttagcggaaa cgccatttca acgtcataca gttgattatt tatatcgtca
    13081 aatctcagcg cacaaagaaa aggttgttga gcaacagttg caattgaaca tgcatgaaca
    13141 aaccattaca gaatttgtgc acgacataaa aacacctgtg acagctatga aattattaat
    13201 tgatcaagaa aaaaatcaag aaagaaaaca agcattacta tatgaatggt ctcgtataaa
    13261 ctcgatgcta gatacacagc tgtatattac tagattagaa tctcaacgta aagatatgta
    13321 ttttgattac gtgtcactta aacgcatggt cattgatgaa atacaattaa caagacatat
    13381 tagtcaggtt aaaggtattg gttttgatgt tgactttaaa gtggatgatt atgtttatac
    13441 agatataaaa tggtgtcgta tgattattag acaaattttg tcaaacgcat tgaaatatag
    13501 tgagaatttt aatattgaaa ttgggacaga attaaatgat caacatgttt cgttatatat
    13561 taaagactat ggcagaggta ttagtaaaaa agatatgccg cgaatatttg aacgaggatt
    13621 tacgtcaacg gctaacagaa atgaaacgac gtcttcaggt atgggtctat atttagtaaa
    13681 tagtgtaaag gatcaattag gtattcacct gcaagtcacg tcgactgttg gtaaggggac
    13741 aactgtcaga ttgattttcc cattacaaaa tgaaattgtt gaacgcatgt cggaagtgac
    13801 aaatttgtca ttttaaacat gcgttttgtt acttagaatt gatacatcaa tgcagcttca
    13861 acgttataat aagatagatg ttagtcatat gttaaattga agatacaagt gccaaagcct
    13921 aaaggaaatg aagttaagat aaattatagg agtgttaaag tggcaatttt agaagtaaaa
    13981 caattaacaa aaatatatgg aactaaaaaa atggcacaag aagtgttgcg agatatcaat
    14041 atgtctattg aagaaggcga gtttattgct attatgggtc cctctggatc tgggaaaacg
    14101 acattattaa atgttttaag ttcaattgat tatatttcac aaggttctat tacattaaaa
    14161 ggaaaaaaat tagaaaagct ttcaaacaag gaattatctg atatacgcaa gcatgatatt
    14221 ggttttattt ttcaagagta taatttactg catacattga ctgttaaaga aaacataatg
    14281 ttaccactaa cggtacagaa gttagataaa gaacatatgt taaatcgtta tgaaaaagta
    14341 gcagaagcat taaatatatt ggatattagt gataaatatc cctctgaatt gtctggtgga
    14401 caaaggcaac gaacatcagc tgccagagca tttataacat tgccttctat tatatttgct
    14461 gacgaaccaa caggtgcatt ggattctaaa agtactcaag atttattaaa acgattaaca
    14521 agaatgaatg aagcatttaa gtctacaatt attatggtaa cgcatgatcc tgttgcagca
    14581 agctatgcaa atcgagtagt gatgctaaaa gatggtcaaa ttttcactga attataccaa
    14641 ggggatgacg ataaacatac ctttttcaaa gaaataatac gtgtacaaag tgttttaggt
    14701 ggcgttaatt atgaccttta acgagataat atttaaaaat ttccgtcaaa atttatcaca
    14761 ttatgccatc tatctttttt cgttaattac gagtgtagta ttgtatttta gctttgtagc
    14821 attaaaatac gctcataaac taaacatgac agagtcatat ccaattataa aggaaggctc
    14881 acaagtcgga agctactttc tatttttcat cataattgca tttttgttat atgccaatgt
    14941 gttatttatt aaacgacgaa gttatgagct tgcatt
//
LOCUS       DAKTGS010000065        14707 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.065, whole genome shotgun sequence.
ACCESSION   DAKTGS010000065 DAKTGS010000000
VERSION     DAKTGS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.065"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(134..1132)
                     /gene="sirC"
                     /locus_tag="O0286_001596"
     CDS             complement(134..1132)
                     /gene="sirC"
                     /locus_tag="O0286_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCY0169976.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1129..2124)
                     /gene="sirB"
                     /locus_tag="O0286_001597"
     CDS             complement(1129..2124)
                     /gene="sirB"
                     /locus_tag="O0286_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCY0169977.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2140..3132)
                     /gene="sirA"
                     /locus_tag="O0286_001598"
     CDS             complement(2140..3132)
                     /gene="sirA"
                     /locus_tag="O0286_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCY0169978.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3363..4343
                     /gene="sbnA"
                     /locus_tag="O0286_001599"
     CDS             3363..4343
                     /gene="sbnA"
                     /locus_tag="O0286_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCY0169979.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4340..5350
                     /gene="sbnB"
                     /locus_tag="O0286_001600"
     CDS             4340..5350
                     /gene="sbnB"
                     /locus_tag="O0286_001600"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCY0169980.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5371..7125
                     /gene="sbnC"
                     /locus_tag="O0286_001601"
     CDS             5371..7125
                     /gene="sbnC"
                     /locus_tag="O0286_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCY0169981.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7118..8374
                     /gene="sbnD"
                     /locus_tag="O0286_001602"
     CDS             7118..8374
                     /gene="sbnD"
                     /locus_tag="O0286_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCY0169982.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8364..10100
                     /gene="sbnE"
                     /locus_tag="O0286_001603"
     CDS             8364..10100
                     /gene="sbnE"
                     /locus_tag="O0286_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCY0169983.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10009..11859
                     /gene="sbnF"
                     /locus_tag="O0286_001604"
     CDS             10009..11859
                     /gene="sbnF"
                     /locus_tag="O0286_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCY0169984.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11834..12610
                     /gene="sbnG"
                     /locus_tag="O0286_001605"
     CDS             11834..12610
                     /gene="sbnG"
                     /locus_tag="O0286_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCY0169985.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12610..13812
                     /gene="sbnH"
                     /locus_tag="O0286_001606"
     CDS             12610..13812
                     /gene="sbnH"
                     /locus_tag="O0286_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCY0169986.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13816..14580
                     /gene="sbnI"
                     /locus_tag="O0286_001607"
     CDS             13816..14580
                     /gene="sbnI"
                     /locus_tag="O0286_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCY0169987.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
BASE COUNT         4878 a         2425 c         2945 g         4459 t
ORIGIN      
        1 taatatgtaa ttaagaaagt cgacgattgt taacaataac aacaactata tgtataaaaa
       61 aacctcgcaa cggttagtta actgaatcat ttagctaact tcgttgtgag gtcattttgt
      121 tttaataata tcgttataac tttttcacgg ttaataataa gtatatgaag aatggggcac
      181 caaaagcagc aataaataca cctgctggca cttctttagg caagaataag gtacgcccaa
      241 ttaagtctgc aataacaatt gatatggcac caatcattgc tgacattagt aactttttag
      301 cataacttcc gcgaacgatt gttttcgcga tatgtggtgc gattaaaccg acaaacccaa
      361 tgttacctac taaactgatt gccatagata cgagtatagt agaagtgatt aattggatta
      421 gtttcatacg ttgtacatgt aagcctaagc caatcgctac agggtcatca agtatagata
      481 ttttcatttt tggtataaca agaaataaca acggcacaac agctaaaata accataccca
      541 aaatgattgt atctttaaac gtagcaccgt aaagacttcc gactagccat gtataagctt
      601 tggcagcaga taattgcttc gttgtaatga gtaatccttg gacaagcgca ataaacaacg
      661 tttgcatcga aataccgatg attatgagtg ttgtcgggcg tatttgtcct ttcgtttgaa
      721 acactaatag tatcatcatt gcaactgcgc cacctaatac tgcaaatagt ggaagtaaat
      781 gtattgttaa atggctgaaa aatgcaataa agacaacagc acttaagcta gcaccacctg
      841 tgataccgat aatatcaggt gaggcaattg gattttttaa tacattttgc aacattaaac
      901 cactcattcc tagtgcggca cctgctaaaa tcgcaagtgt aatgcgaggt aagcgtaata
      961 cttctaaagt gaattgatcc atactgtcat ttggatttat aaagtacatc agtacgcgtt
     1021 gtaatggtat aaagcttgaa ccaatcatca tacttaccac tgaaacgatg gctaaaaaga
     1081 ttaacgcgaa gatgagatgg taattgtctt ttttattaat cttttcggtc ataagcgttg
     1141 acgtcctttc ttcataatat agattaagac aatagcgcca atgacagcgg taacgacacc
     1201 gataggcaac tctagtggct taattattat acgagcaaca atgtctgaaa tgatcattag
     1261 gattgctcca gctaatgcag taaaaggaat taaatactta tagtttggtg gtaataatcg
     1321 tttgctaata ttcggtacga taagacccac aaagacgatt gatccagcta cggctaccga
     1381 aataccggct aacatactga tgagcataat aatcatccat ttgattaatt ttatgttttg
     1441 accgaggccg gttgcaatgt cgtcacttgt catcaagatg ttgatgtgtg cagccatgct
     1501 aaatgcaatt aaaataagta tcaatacaag cggaataatc catgggatat cccaaatatt
     1561 acgtaatgaa acggagccac ttaaccaaaa taataggcct tgtaagtctg tttcgttcat
     1621 aataagtatg ccttgagtaa aggctgtaaa tagcatcgca atcgcagcac ctgccaaaat
     1681 gacacggtga ggtgagaata gtgtttgtct aaacatacct agtgcaacaa ctaatacagt
     1741 aacaacaata gcccccaaaa atgcaataac tacaatcatt ttaaaagatt gaatttggat
     1801 aaatgtaata ctaaaaatga caaaaaatac tgcgcctgca ttgacaccga aaagccctgg
     1861 tgaggctatt gggtttcgtg taagtgcttg catcaacaaa cctgagacag caagggcagc
     1921 accagtcaat aacgcaatga ttgttctcga cgcccgtgca ccagtgacaa catcatgtaa
     1981 atcgttttca ctatcaaagt tgaataacgc ctgtatcacc gtacctggtg acacaagcgt
     2041 atttccaatc attaaactta agatagctac tattgcaaga cataaaccag caataacgat
     2101 ttggtatttt ggtttaagta gcatcgtaaa actccttaat tattttgatt gtttttcaat
     2161 atttaacttt tcatataaat cgtcaataag ttttaatgaa gatttatatc cgccagctaa
     2221 gttccaagtg atttcatcta aatcatcaga tacttggttg tttttaactg cgtctaaatt
     2281 tttccactct ttacttgaag tccattcgct ttcagtcttt ttaactaatg cagcatcttt
     2341 cgcatttgga tctgatttta ctacaaaaat atgatcagcg ttcattaatg gaatgctttc
     2401 tttagatgta agttggataa tatctttacc attatcaact tgtttttgta agtctttatt
     2461 acgtttgaat cctaaatcat ttaagatttc accagcatat ccaccagcat aaattcttgt
     2521 atgatcagca cggaagttaa caactgaagc tttcaatggc catgcatctt tatactttgc
     2581 ttttgcatct ttttggaatg cagctacttt atcatcgtac tttttaagta aatcttcagc
     2641 ttctttttct ttccctaaag ctttccccat taacttagtt gtatctttga atttgaaaac
     2701 tgtatcagta gaaactgttg gtgcgatttt agataattga tcgtaaactt tttcatttct
     2761 aacttttgac gcgacaatta agtccggttt taatttagag atttcctcta agttaggtgc
     2821 aggttcttga cctacaatct tagtatcttt taaatcattt tttatgtatt cgaatttcgg
     2881 tttttgtgtc catgattcta cagcacctac aggtttaaca cctaaagata cagcgacgtc
     2941 agtggcacct tgatatagcg taacaacacg ctttggtttc cctttaattt cagttgtacc
     3001 cattgcatgt ttaattgaag ttgtttcctt atctttgtta tcagatgatt gtttatttga
     3061 attcccacta catcctgcta aaacaagtag gaaagcaagc gtaacaacaa gcattttaat
     3121 tactttattc attgactaat tagcctcctt cgtgatgtat gacaatgaga atcattatca
     3181 cgatttagta tgaattaaat tttttcctaa gtcaataaaa tatttatgat ttacatgcaa
     3241 cttataatta tttgacatat aaatgcataa aaaatataat cctaattact tgatagtgag
     3301 aatcattatc aattaggtaa cacacaatat tatagaattt taaatttgag gaggaagcgc
     3361 ttttgattga aaaaagtcaa gcatgtcacg attcattgtt agattctgta gggcaaacac
     3421 ctatggttca acttcatcaa ctatttccga aacatgaagt gtttgcaaag ttagagtata
     3481 tgaatcctgg aggcagcatg aaagatcgac ctgccaagta catcattgaa catggtatta
     3541 aacatggttt aatcactgag aatacacatt taattgaaag tacttctggt aatttaggca
     3601 ttgcgttggc aatgatagct aaaatcaagg gattaaaact cacgtgtgtt gttgatccta
     3661 aaatatcacc aacaaatttg aaaattatta aaagttatgg tgccaatgta gaaatggttg
     3721 aagaacctga tgcacatggg ggttatttaa tgactcgtat tgcaaaggtg caagaactgt
     3781 tagccactat tgacgatgca tattggatta atcaatatgc gaatgagtta aattggcaat
     3841 cccattatca tggtgcaggc acagagattg ttgaaacaat taagcaacct atagattatt
     3901 ttgtcgcgcc agtcagcacg acaggtagca ttatgggtat gagtagaaaa ataaaagaag
     3961 tgcatccaaa cgcacaaatt gttgctgttg atgcgaaagg gtcagtcatt tttggtgaca
     4021 aacctattaa tagagaatta cctggtatcg gtgctagtcg tgtacccgaa atattgaata
     4081 gatcagaaat taatcaagtg atccatgtag atgattatca atctgctttg ggctgtcgaa
     4141 aactgattga ttatgaaggc atatttgccg gaggttcaac aggttcgatt attgcagcga
     4201 ttgagcagtt gataacgtca attgaagaag gtgcaacaat tgtcacgatt ttaccagatc
     4261 gaggcgatcg ttacttagat ttagtttatt cagatacatg gttagaaaaa atgaaatcaa
     4321 gacaaggagt taaatcagaa tgaatagaga gatgttgtat ttaaatagat cagatattga
     4381 acaagcggga ggtaatcatt cacaagttta tgtggacgca ttaacagaag cattaacagc
     4441 ccatgcgcac aatgattttg tacaaccgct taagccgtat ttaagacagg atcctgaaaa
     4501 tggacacatc gcagatcgaa ttattgcaat gccaagtcat atcggtggtg aacacgcaat
     4561 ttcaggtatt aagtggatag gtagtaagca cgacaatcca tcgaaacgta atatggagcg
     4621 tgcaagtggc gtcattattt tgaatgatcc agaaacgaat tatccaattg cagttatgga
     4681 agcaagttta attagtagta tgcgtactgc agcagtttca gtgattgcag caaagcattt
     4741 ggctaaaaaa ggatttaaag acttaacaat cattggatgc gggctaatcg gagacaagca
     4801 attacaaagt atgttagagc aattcgatca tattgaacgc gtgtttgttt acgatcaatt
     4861 ctctgaagca tgtgcacgct ttgttgatag atggcaacaa cagcgtccgg aaattaattt
     4921 tattgcgaca gaaaatgcta aagaagcagt atcaaatggt gaagtagtca ttacatgtac
     4981 cgtaacggat caaccataca ttgaatatga ttggttacaa aagggtgcat ttattagcaa
     5041 catttctatc atggatgtgc ataaagaagt ctttattaaa gctgacaaag tcgtagtaga
     5101 tgactggtca caatgtaatc gagaaaagaa aactattaac caattggtgt tagaaggtaa
     5161 attcagcaaa gaagctcttc atgctgaact aggacaactt gtgacaggtg acataccagg
     5221 acgtgaagac gatgatgaga tcatattact taatccgatg ggtatggcta tcgaagatat
     5281 ttcaagtgct tattttattt atcaacaggc acaacaacaa aatattggga caacattgaa
     5341 cctatattaa gaatgcgagg tgtctgaaca ttgcagaatc atacagcagt caatacagca
     5401 caagcgataa tattaagaga tttagttgat gcattattat ttgaagatat agccggaatt
     5461 gtatcgaata gtgagattac taaagaaaac ggacaaacgc ttttgatata cgaacgtgaa
     5521 acacaacaaa taaagatacc tgtttatttt agtgctttaa atatgtttcg ttacgaaagt
     5581 tcacaaccaa ttacgataga gggaagggtg tctaagcaac ctttaacggc agctgaattt
     5641 tggcaaacaa ttgctaatat gaattgtgat ctaagtcatg aatgggaagt ggctcgcgtt
     5701 gaagaaggac tgactactgc tgccacacag cttgctaaac aattatcaga attagattta
     5761 gcgtcacatc cttttgtgat gtcagagcag tttgcaagtt taaaagatcg tccatttcat
     5821 ccattagcta aagaaaaaag aggattaaga gaagcggatt atcaagtgta tcaagctgaa
     5881 ttaaatcaat catttccttt aatggttgca gcagttaaaa agacacatat gattcatggc
     5941 gatactgcaa atatcgatga attagaaaat ttgacagtac ctataaaaga acaagcgaca
     6001 gacatgttaa atgatcaagg gttatcaata gatgactatg tactatttcc ggtacatcct
     6061 tggcaatatc agcatattct gccgaacgtc tttgcgaaag agattagtga aaagttggtt
     6121 gtactattac cgttaaaatt tggagattat ctgtcgtctt caagtatgcg ttcattaatt
     6181 gatattggcg caccgtataa ccatgtcaaa gtaccatttg caatgcagtc attaggcgca
     6241 ttaaggctaa cgcctacgcg ttacatgaaa aacggagaac aagcagaaca attattacgt
     6301 cagcttatag aaaaagatga agcactagct aagtatgtca tggtttgtga tgaaacagct
     6361 tggtggtcat atatgggtca agataatgat attttcaaag atcaattagg tcatctaact
     6421 gttcagctaa gaaagtatcc cgaagtgcta gccaaaaatg atacgcaaca gctagtgtca
     6481 atggcagcac tcgcggcaaa tgatcgcact ttatatcaaa tgatttgtgg aaaagataat
     6541 atttctaaaa atgatgtcat gacgttattt gaagatatcg cgcaagtctt tttaaaggta
     6601 acactatcat ttatgcaata cggcgcatta ccagagttgc atggtcaaaa tatattgttg
     6661 tcatttgaag atggacgtgt acaaaaatgc gtgttacgtg atcatgatac tgtcagaatt
     6721 tataaaccat ggctaacagc acatcagctt tcattgccga agtatgtcgt cagagaagat
     6781 acacctaata cgctaattaa tgaggatttg gaaacattct ttgcttattt tcaaacatta
     6841 gctgtatcgg taaatctata tgccattatt gatgcaattc aagatttatt tggtgtaagt
     6901 gagcatgaac ttatgtcgtt gttaaaacaa attttaaaaa atgaagtggc aactatttcc
     6961 tgggttacaa ctgatcagct agctgtcaga cacattttat ttgataaaca gacgtggcca
     7021 ttcaaacaaa ttttattacc attgctatat caacgtgata gtggtggagg tagtatgcct
     7081 tcaggtttaa ctaccgtacc aaatccaatg gtgacatatg attaatcagt ctatatggcg
     7141 cagtaacttt cgcattttat ggctcagtca gtttatagcg attgctggac tgacagtact
     7201 tgtgccatta ttgccaattt atatggcatc actacaaaat ctatcagtcg tagaaataca
     7261 gttgtggagt ggtatagcga ttgctgctcc agctgtaacg acgatgatag cttcgccgat
     7321 atgggggaag ctaggtgata agatcagccg aaaatggatg gtgttaagag cgttacttgg
     7381 tttggcggta tgcttatttt taatggcatt gtgtacgaca ccattacagt ttgtacttgt
     7441 gaggttattg cagggactat ttggtggtgt tgttgatgca tcaagtgcgt ttgcgagtgc
     7501 agaggcgcca gctgaagatc gtggaaaggt attaggaaga ctgcaaagtt cagtcagcgc
     7561 agggtctctt gtggggccat taattggcgg tgttacagct tcgatattag gttttagtgc
     7621 gttactgatg agtattgccg ttattacttt tattgtctgt attttcggtg cattaaaatt
     7681 gattgaaacg acacatatgc caaaatcaca aacaccaaat attaataaag gtattcgccg
     7741 ttcatttcaa tgtctattat gcacacaaca aacatgtcga tttattatcg ttggcgtttt
     7801 agcaaacttt gctatgtatg gcatgctaac tgcattatca ccacttgctt catcagtgaa
     7861 tcatacagcg atagatgacc gtagtgtgat tggattttta cagtccgcat tttggacggc
     7921 ttcgatatta agcgcgcctt tatggggacg ctttaatgat aaatcatatg ttaaatcagt
     7981 atatatattt gccacgattg catgtggttg tagtgcgata ctgcaaggtt tagcgacgaa
     8041 tatagagttt ttaatggctg caagaatact tcaaggatta acatatagtg cattgattca
     8101 aagtgtcatg tttgttgtcg tgaatgcgtg tcatcaacaa cttaaaggca catttgttgg
     8161 aacgacgaac agtatgttag ttgttggtca aattattggc agtcttagtg gcgctgccat
     8221 tacaagttat actacaccag ctactacgtt tatcgttatg ggcgtagtat ttgcagtaag
     8281 tagtttattt ttaatttgtt caaccatcac taatcaaatc aacgatcaca cattaatgaa
     8341 attatgggag ttgaaacaaa aaagtgcaaa ataaagaatt aatacaacat gcagcgtatg
     8401 cggctatcga acgcatttta aatgaatatt ttagagaaga aaatttatat caagtaccac
     8461 ctcaaaatca tcaatggtct atacaattat cagagctcga aactttaacg ggtgaatttc
     8521 gctattggtc tgcgatgggg catcatatgt atcatccaga ggtatggctt atcgatggaa
     8581 aaagtaaaaa aataacaact tataaagaag caattgcgcg tattttgcaa catatggctc
     8641 aaagtgcaga taatcaaacg gcagtgcaac aacatatggc gcaaattatg tctgacatcg
     8701 ataatagcat tcatcgcacg gcacgttatt tgcaaagtaa cacaatagac tacgtagagg
     8761 atcgttatat cgtttcagaa caatctttat acttaggtca tccatttcat ccgactccta
     8821 agagtgcaag tgggttttca gaagcagatt tagagaaata tgcacccgaa tgtcatacat
     8881 cattccaatt gcattattta gctgtgcatc aagatgttct gctcacgcgc tatgtagaag
     8941 gtaaagaaga tcaggttgag aaagtgttgt atcaattagc agacatagat atatcagaga
     9001 tacccaaaga ttttatttta ttaccaacac atccttatca aatcaatgtg ttgcgacagc
     9061 atccacagta tatgcaatat agtgaacaag gtttaataaa agaccttggc gtttccggtg
     9121 attcagtgta cccgacgtct tcggttagaa ctgtattttc aaaagcatta aacatttatt
     9181 taaaattacc gatacacgtt aaaatcacta attttatacg tacgaatgac cttgaacaga
     9241 ttgaacggac aattgatgcc gcgcaagtta tcgcatcagt caaagatgag gttgaaacac
     9301 cccattttaa attgatgttt gaagaaggat atcgtgcatt gttaccgaat ccattagggc
     9361 aaacagttga acctgaaatg gatttattaa caaatagtgc catgattgtt cgtgaaggga
     9421 taccgaatta ccatgctgat aaagatattc atgtattggc gtcattattt gaaacgatgc
     9481 ctgattcacc gatgtctaag ttatcacaag tgattgagca aagtggttta gcaccagaag
     9541 catggcttga atgttatttg aatcgtacat tattgccgat attaaagctg tttagtaaca
     9601 caggcattag tctagaagca catgtacaaa atacattaat tgaattaaaa gatggcatac
     9661 ccgacgtatg ctttgtcaga gatcttgaag gcatttgtct atctagaacg attgctactg
     9721 aaaaacagct tgtgccaaat gttgtggcag catcaagccc tgttgtatat gcacatgatg
     9781 aagcatggca tcgtcttaaa tattacgttg tagtaaatca cttaggacat ttagtatcaa
     9841 ctattggtaa agcgactaga aatgaagttg tgttatggca acttgtagcg catcgtctta
     9901 tgacttggaa aaaagaatac gcgaataacg cagtatttgt tgactgtgta gaagatttat
     9961 atcaaacgcc gaccattgcg gctaaagcga atttgatgag taaattgaat gattgtggtg
    10021 caaaccctat ttatacacat ataccaaatc caatttgtca taacaaggag gtatcgtatt
    10081 gtgaatcaaa caattcttaa tcgtgtaaag actagagtga tgcaccaact ggtatcatca
    10141 cttatttatg agaatattgt tgtgtataaa gcgtcatatc aagacggtgt cggtcatttt
    10201 acaatagaag gacatgattc agagtatcgt tttactgctg aaaagacaca tagctttgat
    10261 cgtatacgta tcacatcacc aattgagcgt gtcgtaggag atgaggcaga tacaacaaca
    10321 gactatacac aattattgag agaggttgta tttacatttc ctaaaaatga tgaaaagcta
    10381 gaacaattta ttgtcgagtt attacagaca gaattaaaag atacgcaaag tatgcagtat
    10441 cgagaatcaa acccaccagc aacacctgag acatttaacg actatgaatt ttatgcgatg
    10501 gaagggcatc agtatcatcc aagttacaaa tcacgtttag gatttacgtt gagtgataat
    10561 ttgaaatttg gtcctgattt tgtaccaaac gttaaactgc agtggttagc tatcgacaaa
    10621 gataaagtag aaacgacggt atcaagaaat gttgtagtta acgaaatgtt acgtcaacaa
    10681 gttggcgata agacttatga acattttgta cagcagattg aagcatctgg caaacatgta
    10741 aatgatgttg agatgatacc tgtacaccca tggcagtttg aacatgtcat ccaagttgat
    10801 ttggctgaag aaaggcttaa tggcacagta ctatggttag gggaaagtga tgagctatat
    10861 caccctcaac aatcgattcg tacgatgtcg ccaatagaca cgacaaaata ttatttaaag
    10921 gtaccaataa gtataacgaa cacttcaacg aaacgagtgt tggcgcctca tacaattgaa
    10981 aatgcagcgc aaattacgga ttggttaaag cagatacagc aacaagatat gtatttaaaa
    11041 gatgaattaa agacagtttt tctaggggaa gtcttaggac agtcttattt aaatacacaa
    11101 ctttcgcctt ataaacaaac tcaagtttat ggtgcgttag gtgttatatg gcgtgaaaat
    11161 atatatcata tgttaatcga tgaagaggat gcgataccat ttaatgcact ttatgcaagt
    11221 gataaggatg gtgtaccatt cattgaaaat tggattaaac aatatggttc tgaagcttgg
    11281 acaaagcaat ttttagctgt agcgattcgt ccaatgattc atatgcttta ttatcacggt
    11341 attgcctttg aatcgcatgc acaaaatatg atgctcattc atgaaaatgg ttggcctaca
    11401 cgtattgcct taaaagattt ccatgatggt gttcgtttta agcgtgagca tttaagtgaa
    11461 gctgcttcac acctgacatt aaagccaatg ccagaagcac ataaaaaagt gaatagtaat
    11521 tcatttattg aaacagatga cgaacgttta gtacgcgact ttttacatga tgcatttttc
    11581 tttattaata tcgccgaaat catcttattt attgaaaagc aatatggtat cgatgaggag
    11641 ctgcaatggc aatgggttaa aggcatcatc gaggcgtatc aagaagcatt tccagagttg
    11701 aataactatc aacatttcga tttgtttgaa cctacgattc aagttgaaaa gttaacgaca
    11761 cgtcgattat taagtgactc cgagttaaga attcatcatg ttacaaatcc attaggtgta
    11821 ggaggtatca atgatgcaac aactatctct gaaacataga ttaaacaatg gtgattcagt
    11881 ttatggcatt tttaattcta taccggaccc attgatgatc gaggttatcg cagcaagcgg
    11941 gtatgacttt gttgtgattg atacagaaca cgtggcgatt aatgatgaga cactagcgca
    12001 tttaattcgt gcagctgaag cagcgcatat tataccaatt gtacgtgtca ctgcagtgat
    12061 agatagagat atcattaaag tgttggatat gggtgcgaga ggtattattg tgccacacgt
    12121 taaagatcgt gagacagttg agcatattgt gaaattaagt cgttattacc cgcaaggatt
    12181 aagaagtttg aatggtggtc gcatggcaag atttggacgt acaccattac ttgatgcaat
    12241 ggagatggct aatgagcata ttatggtgat tgccatgata gaagatgttg aaggggttat
    12301 ggccattgac gatatagcac aagtcgaagg tttagacatg atagtcgaag gtgccgcaga
    12361 tttatcgcag tcacttggca taccatggca aacgcgtgat gatcaagtaa catcacatgt
    12421 tcaacatatt tttgaagttg tgaatgcaca tggtaaacat ttttgtgcat taccacgtga
    12481 agatgaagat attgcaaaat ggcaggcaca aggtgtacaa acatttattt taggtgatga
    12541 tcgcggaaaa atatatcgcc atttaagtgc atctctagcg acgtctaaac agaaagggga
    12601 tgaaggctaa tgcgtatagt tcaacctgtt attgaacaat taaaagcaca atctcatcca
    12661 gtttgtcatt atatctatga tttagtcgga ctggaacatc atttgcaaca tattacatcg
    12721 tcattgccga gtaattgtca aatgtactat gcaatgaaag caaatagtga acgaaaaatc
    12781 ctagatacaa ttagtcagta tgttgaagga ttcgaagttg catctcaagg tgaaatagca
    12841 aaaggtcttg cttttaaacc agcaaatcat attatttttg gtggccctgg taagacagac
    12901 gaggaactaa gatatgcagt aagtgaaggt gttcagcgta ttcatgttga aagtatgcat
    12961 gaattacaac ggctaaatgc catcttagaa gatgaagata agacacaaca cattttattg
    13021 cgtgttaatt tagcaggacc atttcccaat gcaacgttgc atatggcagg acgcccaaca
    13081 caatttggta tttctgaaga cgaagttgat gatgtcattg aagctgcgct cgcaatgcca
    13141 aagattcatc tagatggatt tcattttcat tctatttcta acaatttaga ctcgaattta
    13201 catgtcgatg tagtgaaact ttattttaaa aaagcaaagg catggtctga aaaacatcga
    13261 tttccactca aacatatcaa tcttggtggt ggcataggcg ttaactatgc agatttaact
    13321 aaccaatttg aatgggataa ttttgtagaa cgttttaaaa cacttatcgt tgagcaagaa
    13381 atggaagatg tgacattgaa ctttgaatgt gggcgcttta ttgtggcaca tattggttac
    13441 tatgtgacag aagtgctaga cattaagaaa gtacatggtg cttggtatgc catattaaga
    13501 ggaggtacgc aacaatttag actgccggta tcttggcaac ataaccatcc ttttgacatt
    13561 tatcgctata aggacaatcc atattcattt gaaaaagttt caatttcgag acaggacaca
    13621 acgttagtcg gtcaattatg tacaccgaaa gatgtctttg ctagagaagt acagatagac
    13681 gcaatcagta caggcgacgt tattgttttc aaatatgcag gtgcatacgg atggtctatt
    13741 tcacatcacg atttcttaag ccatccacat cctgaattta tttatttaac gcaaacaaag
    13801 gaggatgaat aactattgaa tcatattcat gaacatttaa aattggtacc agtagataag
    13861 attgatcttc acgaaacatt cgaaccttta agattggaaa aaacgaaaag tagtattgaa
    13921 gcagatgatt ttatacgtca tcctatttta gtgacagcga tgcaacatgg tagatatatg
    13981 gttatagatg gtgtgcatcg gtatacaagt ttgaaagcgt taggatgtaa gaaagttcca
    14041 gtgcaagaaa tccatgaaac acaatattca attagtacat ggcaacataa agttccattt
    14101 ggtgtgtggt gggaaacgtt acaacaagaa catcgcttgc catggactac tgagacaaga
    14161 caagaagcgc catttattac aatgtgtcat ggtgatacag aacaatattt gtatacaaaa
    14221 gatttaggcg aagcacattt tcaagtatgg gaaaaggttg tcgcaagtta tagtggttgt
    14281 tgttctgtag agagaattgc acaaggtaca tatccttgtc tttctcaaca agatgtactc
    14341 atgaagtatc agccattgag ttataaggaa attgaagcgg ttgttcataa aggggaaact
    14401 gtgccagcag gtgtgacacg ctttaatatt tcaggacgat gtcttaatct tcaagtacca
    14461 ctggcattac ttaaacaaga tgatgatgtt gaacaactgc gcaattggaa gcagttttta
    14521 gcagataagt ttgccaatat gagatgctat actgaaaaag tatacttggt ggagcaatag
    14581 ttttactgtg atgttgaggg aaatatgatg atttagcgta ttgatagcga aaatataata
    14641 aaacaatata gtgtggagaa cttttgatat tttataaata ttgaagttct ccatttttgt
    14701 attttgc
//
LOCUS       DAKTGS010000066        14678 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.066, whole genome shotgun sequence.
ACCESSION   DAKTGS010000066 DAKTGS010000000
VERSION     DAKTGS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.066"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            61..852
                     /locus_tag="O0286_001608"
     CDS             61..852
                     /locus_tag="O0286_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0169988.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            1220..1648
                     /gene="lacA"
                     /locus_tag="O0286_001609"
     CDS             1220..1648
                     /gene="lacA"
                     /locus_tag="O0286_001609"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCY0169989.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            1664..2179
                     /gene="lacB"
                     /locus_tag="O0286_001610"
     CDS             1664..2179
                     /gene="lacB"
                     /locus_tag="O0286_001610"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCY0169990.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            2192..3124
                     /gene="lacC"
                     /locus_tag="O0286_001611"
     CDS             2192..3124
                     /gene="lacC"
                     /locus_tag="O0286_001611"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCY0169991.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            3128..4108
                     /gene="lacD"
                     /locus_tag="O0286_001612"
     CDS             3128..4108
                     /gene="lacD"
                     /locus_tag="O0286_001612"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCY0169992.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            4130..4441
                     /locus_tag="O0286_001613"
     CDS             4130..4441
                     /locus_tag="O0286_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCY0169993.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            4447..6159
                     /locus_tag="O0286_001614"
     CDS             4447..6159
                     /locus_tag="O0286_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0169994.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            6177..7589
                     /gene="lacG"
                     /locus_tag="O0286_001615"
     CDS             6177..7589
                     /gene="lacG"
                     /locus_tag="O0286_001615"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCY0169995.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            7855..8724
                     /locus_tag="O0286_001616"
     CDS             7855..8724
                     /locus_tag="O0286_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0169996.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            9007..10008
                     /locus_tag="O0286_001617"
     CDS             9007..10008
                     /locus_tag="O0286_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCY0169997.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            10242..11243
                     /locus_tag="O0286_001618"
     CDS             10242..11243
                     /locus_tag="O0286_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0169998.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            11550..13112
                     /locus_tag="O0286_001619"
     CDS             11550..13112
                     /locus_tag="O0286_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0169999.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            13287..13823
                     /gene="amaP"
                     /locus_tag="O0286_001620"
     CDS             13287..13823
                     /gene="amaP"
                     /locus_tag="O0286_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCY0170000.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            13836..14075
                     /locus_tag="O0286_001621"
     CDS             13836..14075
                     /locus_tag="O0286_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCY0170001.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            14138..14647
                     /locus_tag="O0286_001622"
     CDS             14138..14647
                     /locus_tag="O0286_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0170002.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT         5170 a         2116 c         2870 g         4522 t
ORIGIN      
        1 cgcaattttt attatagaat aagcatgtat attaaaattg atgtctaaac ataaaggata
       61 atgacccatc gcctaaataa aggaggcaat tttacattga ataaacatga acgtttggac
      121 gaaattgcta aactagtgaa taaaaagggc acgataagaa cgaatgaaat cgtcgaaggt
      181 ttaaatgtgt ctgatatgac agttcgaaga gatttaattg aattggaaaa taaagggatt
      241 ttaacgaaga ttcatggtgg tgcacgcagt aattcaacat ttcagtataa agaaatatcg
      301 cataaagaaa aacatacccg tcaaattgca gaaaaacgat ttatagctaa aaaagctgca
      361 tcattaattg aagatgggga tactttgttt ttcggaccag gaacaacagt ggaactatta
      421 gcagaagaag tcaatcatca tacgctcaca attattacga attgtttgcc ggtgtataaa
      481 attttgttag aaaaacaaac agcacatttt cgtgtctatt taattggtgg tgaaatgcgc
      541 catattacag aagcatttgt aggtgaaatg gcgaacgcca tgttggaaaa actaagattt
      601 agcaagatgt tctttagtag taacgcagta aataaaggtg ctgtgatgac atctacatta
      661 gatgaagcct atacgcaaca actggcacta agtaattcaa ttgaaaaata cttgttaatc
      721 gatcatacga aagttggcaa agaagatttt acatcatttt gtcagttaaa cgaattgact
      781 gcagtggtca tggactatga agatgaagaa aaagtagaaa cgattaaaac atatattgaa
      841 gtagtcgact aaattaaaag tataactgca ttgttaaaat atgtatctat atgctcataa
      901 gtgaatgtga atttgcattc atttatgggc tattttttat gcaaatttat gtcaacggga
      961 acctcgacaa agttttacta caatttcaga cgaataccta attaaaacaa caccacaata
     1021 tgaatgttca cttattgttt gaaaatacgt cataaacaaa catattataa acatattatg
     1081 ttgatttatt gtttgtttat gtttataatt taaacgtaat caaattgaaa gccttttctc
     1141 aagattttca taacctacaa ataaatatgt cacattttaa gaaaggtatt tcaaaattaa
     1201 agtaaaaagg agtcttatta tggcgattat tattggttca gatgaagctg gcaaacgatt
     1261 aaaagaagtc atcaaatcat acttattaga caataaatat gatgttgttg acgtaacaga
     1321 aggacaggaa gttgactttg ttgatgcaac tttggctgta gcaaaagatg ttcaaagtca
     1381 agaaggtaac ttaggtattg ttatcgatgc atttggtgca ggtagcttta tggttgctac
     1441 aaaaatcaaa ggcatgattg ctgctgaagt atcagatgaa cgctctggat atatgactag
     1501 aggccataac aattcaagaa tgatcactat gggatctgaa attgttggag acacattagc
     1561 gaaaaatgtc gttaaaggtt ttgttgaagg taaatacgat ggtggtagac accaaatccg
     1621 cgtagatatg cttaataaaa tgtgctaagg aggaaaaatt actatgaaga ttgcattagg
     1681 atgcgaccat attgttacag atacaaaaat gcgtgtatct gaatttttaa aatcaaaagg
     1741 acatgaagtc attgacgtag gaacatacga tttcacaaga acacattatc caatttttgg
     1801 taaaaaagtt ggcgaacaag ttgttagcgg taatgcagac ttaggtgttt gtatttgtgg
     1861 aacaggtgtt ggtattaaca atgctgtaaa taaagtacct ggcgttcgtt cagcactagt
     1921 acgtgatatg acatcagcgt tatacgctaa agaagaatta aatgcgaacg ttattggctt
     1981 cggtggacgt attataggtg agttattaat gtgcgatatt atcgatgcat ttattaatgc
     2041 tgaatataaa ccaactgaag agaacaaaaa attaatcgct aaaattaaac atttagaaac
     2101 aagcaatgca gatcaagctg atccacattt ctttgatgaa ttcttagaaa aatgggacag
     2161 aggcgaatac cacgattaag ggacgacaca tatgatttta actttaacat taaatccatc
     2221 cgttgatatt tcatacccat taacagcatt gaaattagat gatgttaatc gtgttcaaga
     2281 agtaagtaaa acagctggtg gtaaaggttt aaatgtaaca agagtacttg cgcaagtagg
     2341 tgagccagtc cttgcgagtg gttttattgg cggtgaatta ggtcaattta ttgctaaaaa
     2401 attagatcat gccgacatca agcatgcatt ttataacatt aagggtgaaa cacgaaactg
     2461 cattgcaatt ttacatgaag ggcaacaaac agaaatttta gagcagggac cggagattga
     2521 taatcaagag gctgcaggat ttattaaaca ttttgaacaa ttattagaaa aagttgaagc
     2581 agttgctatt tcaggtagct tacctaaagg attaaatcaa gattattatg cacaaatcat
     2641 tgaacgatgc caaaacaaag gtgtgcctgt gatattagat tgctcgggtg caactttgca
     2701 aacagttttg gaaaatccat acaaacctac agtcattaaa ccaaacattt ctgaactata
     2761 tcaattgctt aaccagccac ttgatgaaag tttagaaagt ttgaaacagg cagtgtcaca
     2821 acccttattc gaagggattg agtggattat tgtgtcttta ggtgcacaag gtgcattcgc
     2881 taagcacaat catacattct atagagtgaa tattccgaca ataagtgtat taaatccggt
     2941 tggttcaggt gattctacag ttgctggaat tacttcagca attcttaatc atgaaaacga
     3001 ccacgattta ctaaaaaaag ccaatacttt aggcatgctg aatgcacaag aagcacaaac
     3061 aggttatgtc aatttaaata attatgatga tttattcaat caaattgaag ttttagaggt
     3121 gtaaaaaatg tcaaaatcga atcaaaaaat cgcatcaatt gaacaattaa gtaacaacga
     3181 aggtatcatt tctgcattag catttgacca acgtggtgct ttaaaacgca tgatggcaaa
     3241 acatcaaact gaagaaccaa ctgtagctca aattgaacaa ttaaaagtat tagttgcgga
     3301 agaattaaca caatatgcat cttcaatttt attagatcct gaatatggtt taccagcttc
     3361 agatgcacgt aacaaagatt gtggattatt acttgcttat gaaaaaactg gatatgacgt
     3421 aaacgctaaa ggtcgtttac ctgactgttt agtagagtgg tctgcaaaac gtttaaaaga
     3481 acaaggcgca aatgctgtta aattcttact ttactatgat gttgatgatg ctgaagaaat
     3541 taacattcaa aagaaagcat acattgaaag aatcggttca gaatgtgttg cagaagatat
     3601 tccattcttt ttagaagtat taacatacga tgacaacatt cctgataacg gtagtgttga
     3661 attcgcaaaa gttaagccta gaaaggttaa cgaagcaatg aaattattct cagaaccacg
     3721 ctttaacgtt gatgtattga aagttgaagt accagtaaac atgaaatatg tagaaggctt
     3781 tgctgaaggt gaagtggttt atacaaaaga ggaagcggca caacacttta aagatcaaga
     3841 tgcagcaact catttaccat acatttactt aagtgctggt gtttctgcgg aattattcca
     3901 agaaacttta aaatttgctc acgaagctgg tgctaaattc aacggggtac tttgtggccg
     3961 tgctacatgg tctggtgctg ttcaagtata cattgaacaa ggtgaagatg ctgctcgtga
     4021 atggttacgt acaactggtt tcaaaaatat tgatgactta aataaagtat taaaagatac
     4081 tgcgacatct tggaaacaaa gaaaataagt ttaaaagggg attaatgcta tgaatagaga
     4141 agaagtccaa ttattaggtt ttgaaattgt tgcatttgca ggggatgcac gttctaagtt
     4201 tttagaagca ttaacagcag ctcaagctgg agattttgca aaagcagatg cactgattga
     4261 agaaggaaac aattgcattg cggaagcgca tagagcacaa acaagcctgt tggctaaaga
     4321 agcgcaaggt gatgatattg catacagtgt aacgatgatg catggtcaag atcatttaat
     4381 gacaacaatt ttactgaaag atttaatgaa gcatttatta gaattttata aaagagggtg
     4441 atgacaatga tgcaaaaatt gatcgcacaa attgaaaaag ggaagccatt tttcgagaaa
     4501 ttatcacgta acatatattt aagagccatc cgtgatggat ttatttctgc aatgcctgtc
     4561 atcttattct caagtatctt tctattaatt gcatatgtgc caaatatatt tggttttaaa
     4621 tgggataaag gcatggaagc cattttaatg aaaccataca actatacgat ggggcttgta
     4681 gcatttttag tagcaggaac gacagcgaaa tctttaacag attcatttaa ccgtaagcta
     4741 gaaagtacaa atcaaattaa ctttatttct acaatgctag cagcgatgtg tggattcttg
     4801 ttcttagctt ctgatccagc caaagatggt ggattcttaa gtgcatttat gggaactaaa
     4861 ggtttattaa cagcattctt atcagcattt gtaactgtta ttgtttataa cttctgtgtg
     4921 aagcgcaata ttacaattaa aatgccgaaa gaagtaccgc cgaatatttc acaagtattt
     4981 aaggacttaa ttccattttc agcggtaatc atcattcttt atgcattaga tttagtcatt
     5041 cgcaacagct ttaaatcaaa tgtagcggaa ggtattttaa aattattcga accattattt
     5101 acagcagcag atggatggat tggtgtcaca attatctttg gtgcctttgc attattctgg
     5161 tttgtaggta ttcatggtcc gtcaattgta gagccagcaa ttgcagccat tacatatgcg
     5221 aatatcgaag cgaacttcaa gttgcttcaa gctggagaac acgcagataa aattattaca
     5281 tcaggtacac aaatgtttat cgttacattt ggaggtacgg gtgcaacatt ggtcgtcccg
     5341 ttcatgttta tgtggatgac gaaatctaaa cgaaacaaag cgattggacg tgcatcagtt
     5401 gtaccaacat tctttggtgt aaacgaacca attttatttg gtgcaccact agtattgaat
     5461 cctgtgttct ttattccatt tgtattagca ccaattgtta acgtatggat tttcaaactt
     5521 ttcgttgaag tgttaggaat gaatagtttt agtgtgaatt taccttggac aacaccaggt
     5581 ccattgggca ttatcatggg tacaggtttt ggtttatggt cattcgtact agctattact
     5641 ttgattgttg tagatattat tatttactac ccattcctaa aagtttatga tagtgaaatt
     5701 cttgatgaag aagaaggacg taaagaaagt aattcagatt taaaagaaaa agttgcagca
     5761 aactttgata cgaaaaaagc tgattcaatt ttagcggcaa gtggtgtatc agacgatgca
     5821 gctaaagctt caaatatcac agaacaaaca aatgtcttag tactttgtgc cggtggtggt
     5881 acaagtggat tattagccaa tgcacttaat aaagcagctg aagaatatca tgtgccagtg
     5941 aaagcggcag ctggcggata tggcgcacat atggacatta tgaaagagta ccaattaatc
     6001 attttagcgc cacaagttgc gtctaactat gaagatatta agcaagatac agatagatta
     6061 ggtattaaat tagcgaagac tcaaggtgct gaatatatca aattgacacg agatggccaa
     6121 gcagcccttg atttcgttca acaacaattt gaaaattaag aataggagtt tttcatatga
     6181 ctaaaacatt acctgaagat tttatttttg gtggagcaac agcagcatat caagcagaag
     6241 gtgcgacaaa tacggatggt aaaggtcgcg tagcatggga tacgtattta gaagaaaact
     6301 attggtacac tgcagaacca gcgagtgatt tttataacag atatccagtg gatttagaat
     6361 taagtgaaaa attcggtgtt aatggtatcc gaatttcaat tgcatggtct cgtatcttcc
     6421 caaatggata tggcgaagtt aaccctaaag gtgttgagta ttaccataag ttatttgcag
     6481 agtgtcataa acgtcatgtt gaaccatttg taacattgca tcattttgat acaccagaag
     6541 tgttacacaa agatggcgat tttctaaatc gtaaaacgat tgactacttt gtagattatg
     6601 ctgaatattg ttttaaagaa ttcccagaag taaaatattg gactacattc aatgaaattg
     6661 gacctattgg tgatggtcaa tatttagtag gtaaattccc tccaggtatt aaatatgatt
     6721 ttgaaaaagt attccaatca catcataaca tgatggtggc tcatgctaga gcagtaaaat
     6781 tatttaaaga tggcggatac aaaggagaaa ttggtgttgt acacgcattg ccaacaaaat
     6841 atccgttcga tccatcaaat cctgaagatg ttcgtgcagc agaattagaa gatatcatcc
     6901 ataataaatt catcttagat gcaacatatt taggtaagta ttctcgtgaa acgatggaag
     6961 gtgtgcaaca tatcttatct gttaatggcg gtaaattaaa cattacagat gaagattatg
     7021 ccattttaga tgcagccaaa gatttaaacg acttcttagg tatcaattac tacatgagtg
     7081 attggatgag aggttacgat ggagaatctg aaatcacgca taatgcgaca ggtgataaag
     7141 gcggttctaa ataccaactt aaaggtgtcg gacaacgtga atttgatgtc gacgtacctc
     7201 gcacagactg ggactggatg atctatcctc aaggtttata tgatcaaatc atgcgtgttg
     7261 ttaaagatta tcctaactat cataagattt atatcactga aaatggttta ggatataaag
     7321 atgaatttat tgaatctgaa aaaacagttc atgatgatgc acgtattgat tatgtaagac
     7381 aacatttgaa cgttattgca gatgctatta tagatggtgc aaatgttaaa ggttacttta
     7441 tttggtcatt aatggatgtg ttctcttggt caaatggtta tgaaaaacga tatggtttat
     7501 tctatgtcga ttttgagact caagaacgtt atcctaagaa aagtgcttat tggtacaaag
     7561 agttagccga aacaaaagaa attaaataat tatagatatt aaaaggccta tcagtcgagt
     7621 gctggtaggt ctttatttat agaaagagat aaattaaaca tgcatataca tatttatata
     7681 ttaatcaatg ggtgacacac cattgaaagc taaagaagca atcaattaac aaacgttaac
     7741 ggacaacttt ccaaagtcat cttaaaagtt aatgatgtat tcaaaatcaa ctaaaggaca
     7801 tttttaatca catcctttat acttatataa aagacatgaa tgggagttaa aagtgtgaag
     7861 aaaatttttg taattattac gacattgtta gcggtcgcta ttattatagg ctctattatt
     7921 atggtggtgt ttagtcaacg acaggcacaa acatttaaaa tacaacaaca gcaatttgtt
     7981 aaaaagccaa ttccaacatt gttcttacat ggatttggtg gtagtgccaa ttctgaaaag
     8041 tttatggtga agcaagcaga aaaaagaggg gtaactaaag atatcatcac tgcttacgtt
     8101 tcaaaggatg gtgcagtgac atttaaaggg aaactaagaa aagatgcagt gaatcctatt
     8161 gtgaaaattg aacttgaaaa taacagacaa ggctatttgg ataagaatgc tgcgtggttt
     8221 aaaaatgtac tgactaagtt acaatctgaa tacaatttcg ataaattcaa ctttgtcggt
     8281 cactcaatgg ggaacttaac atttgcacaa tatatgatga catatggtaa cgacaagtca
     8341 ctgccacagt taaacaaaca agtgaatatt gcagggacat ttaatggtgt tttaaatatg
     8401 aatgaagatg tcaatgaaat tacagttgat aaagatggta aaccaagtcg aatgaatcaa
     8461 ccttatcagc aattacgcgt gctaaaagat atttataaag gtaaaggtat cgaagtgcta
     8521 aatatttatg gggatttgaa agatggtaca cattcagacg gcagagtgtc taatagttct
     8581 tcgaaatcat taaaatattt actgggaaat agtccgaaaa gttatcgaga atctaaatat
     8641 gaaggtgagc ctgcacaaca tagtcagctt catgagaatg aaaatgttgc taatgagtta
     8701 atcgacttct tatggaaaaa ataaagcaac tgctagtttt tctggggcaa aaaagcttgt
     8761 aatattagag cttttcaaaa attatctatt gtttgagagt ttccataaaa tgtctaagtc
     8821 gtaacatttt aaatttatga ttatagcaaa tataatgtgg ttgaagttac atgaatgaat
     8881 cagtgcttaa gtcaaaatat aacaactaag aatgtatttg catacagaca tcacgaagac
     8941 acaattcaaa cttaacgctt aagtaacaaa taagtaaaag tttcaaataa agaaaggtga
     9001 ttaatgatgc aaaataaaca aattcttttt aacaaaatac cagaaggcat gcctcaagag
     9061 gatacattca aaattgaaga gattgacacg cctaaacttg agagcgatgg tgtacttgta
     9121 caaacgctgt atatttcagt ggatccatat atgagaggac gtatgactaa ggcagattcg
     9181 tatgtacagc cttttgaaat tggtaaaccg attgttagcc atgtcgttgc aaaagtaatt
     9241 gattcgacac tcgcagatta taaaaaagga gatgtcgttg taggtatgtt gccttggcga
     9301 atcattaatc atgtccaagc ggatcaaatt actaaagttc caacgacaga tgtaccactt
     9361 gatttatatt taagtgtttt aggaatgcca ggacaaacag catatcatgg tctgcttgat
     9421 attggacaac ctaaagccgg tgatactgtt gtggtttcag ctgcatctgg tgctgtaggt
     9481 tcagttgtag gtcaaattgc taagattaaa ggttgccgtg tagtaggtat tgcgggtggc
     9541 gacaaaaaag taaattattt aacagagaca ctgggctttg acgctggtat tgattataag
     9601 aaagaggatt ttgcagaagc gttagcacaa gcagtaccag atggtattga tgtttatttt
     9661 gaaaatgttg gtggaacagt tggcgatgaa gtatttaagc atttaaatcg ttttgcacgt
     9721 gtaccagtat gtggcgcaat ttcgtcatat aatcatcctg aagccgatat cggaccacgt
     9781 attcaaggta cgcttattaa gaagcaagta atgatgagag gcttcttggt aagtgaattc
     9841 gctaatgctt ttaaagaagc tagtgaacag cttgcaacat gggttcaaga aggtaaaatt
     9901 cagtcacaag taaccattga agatggtttt gaaaatgcgc cacatgcatt taaaaattta
     9961 tttactggcg ataactttgg taaacaagtt attaaagtaa cggaataata aagtatacgt
    10021 tttaacaaac taactttgtt aattgaccaa aatttaataa ttttttatct aaagtaaatt
    10081 tgttcagcaa catggtagaa ataagtctaa aatttaatac gaaagtcgtc tgtaattatt
    10141 tatatattca aatgaaaaag gagtcatgat gctgcgatat aagcacacat ggcttctatt
    10201 ttgtataata agaaataagt tcataaatga ggtgaaacga tatgaaaatg ataggatttg
    10261 agaagccctt taaattggaa gaaggaaatt tatttaaagt atacgaacaa agaaagccaa
    10321 cgccagaaaa cgatgatatc ttagtcaaag taaatagcat tagtgtaaat cctgtagata
    10381 caaagcaacg acaaatggaa gtcactcaag cacctcgtgt acttgggttt gatgctattg
    10441 gtactgttga agctatcgga ccagacgtta cattatttag tccgggtgac gttgtatttt
    10501 atgctggatc gccaaataga caaggatcaa atgcgacata tcaattggtg tcagaagcta
    10561 tagttgcaaa agcaccacac aacataagtg ctaatgaagc ggttagtcta ccgctaactg
    10621 gtattacggc atatgaaacg ttttttgata catttaaaat ttctacgaat cctgctgaaa
    10681 acgaagggaa gtctgtctta atcattaatg gtgccggtgg tgtaggcagt atagccactc
    10741 aaattgctaa acggtatggt ttaactgtca ttacaacagc atcacgacaa gaaacaacag
    10801 agtggtgtga aaagatgggc gcagatatcg tattgaatca taaagaggat ttagtgcgtc
    10861 aatttaaaga aattccatta gtagattata tattttgcac ctataataca gacttgtatt
    10921 acaatactat gattgaactg ataaaaccat tagggcatat tacgactatc gtagctttta
    10981 atgaagatca agatttaaat gcattaaaat taaaaagtat aacatttaca catgaattta
    11041 tgtttgcgcg tccaattcat cgaacgcctg atatgattaa acaacatgag tatttagaag
    11101 atattacgaa aaatatagaa tcagggcatt accaaccaac aacgacacaa gtttttgaag
    11161 gtttatcacc tgaaaactta tatcaagcac atcaattgtt ggaaaaacaa tcaatgattg
    11221 gaaaattagt aattaatata tagattagta agtaaagtcg ccttatgcag aaaatactgt
    11281 ataggcgact tattttttgt gtctagcgac tattgattct aaacggaaat atggcaactt
    11341 tgcaggtatc aaatcaataa tgcactagaa aggcattact taaatacaat aaaatacttt
    11401 gttgctcaat gataaataat ttgagtgtgt tttatatatc atttttaaaa gatgaggcat
    11461 tattaattgt gtctatataa aattacaaat gattaaaatg ttgcagttat gggtatgaac
    11521 ttaccattac acagaaaggg tttgtattta tgaaaaataa atcagtaagt ctagtgtttt
    11581 gggtatcttt agtgatatgt acgatatttg ttgcttttgg agcaatattt ccgaaacagt
    11641 tagaaaaact cacgcaaaac attactacat ttattgcgct acatttttct tggtattatt
    11701 tattgctcgt tttagttatt ttatttgtct gtgtgtatat tttattttcg agatacgcaa
    11761 acattacatt aggtgaagaa ggcgaagatc ctgaattctc attgccgtct tggtttgcta
    11821 tgttatttag tgctggtatg ggaataggac ttgtgttctg gacaacggca gaaccaatca
    11881 gtcatgcctt caaattaacg cctattcata aagctggcac gcaatctgca attaatgatg
    11941 caatgcagtt ttcatttttc cattggggga ttcatgcatg ggcagtctat ggtattgtcg
    12001 cgctagtgtt tgcatatttt agctttcata aaggatatcc tggcttagtc agcgctacat
    12061 tgacgccttt attaggtgaa aaggcaatgc gtggaccttt aggtggtgcc attgatgtat
    12121 tggcagtcat agctacagta acaggcgttg ctgcaacatt aggtttcggt gcattgcaaa
    12181 taaacgaagg tttacatttt ttatttaatg taccgagtaa cttcacgatg caagtaatct
    12241 tgattgtcat tgcaaccata ttatttactt ggtcagcatg gtcaggtatc gataaaggta
    12301 tcaaaacgtt gagtaatatc aacatgttac ttgcttttgt agtacttatt ggtcttttta
    12361 ttgttgggcc aacgctatac attttaaata catttacgaa tggtctaggt aattatattg
    12421 caaatttctt tagtatgagt ttacgcattc cgacaggtgg tcaaaaattc caatggttac
    12481 aaaattggac aatcttttac tgggcatggt ggatatcttg ggcaccattt gtaggtatat
    12541 ttattgcacg tgtgtcaaaa ggacgtacga ttaaagaatt tattctaggt gtactttttg
    12601 tcccggcgtt agtttgcttt atattctttg cggtattcgg cgcgtcagca atttacttac
    12661 aagataatca tattgccgat attgctaaag ctgcgacaga aacagcaact tttgcgacgc
    12721 tacaacatta tccgctaggt tttgtattaa gcttaattac tttacttgtt attatgattt
    12781 tctttgttac atcagcggat tcggcaactt atgttttagg tatgttgagt tcaagtggtg
    12841 atattaatcc gaagtcattt gtcaaagtga gttggggtat cattatggca ttatttgcaa
    12901 tcatcatgat ttacacaggt ggtactcaag caattcaaaa tttattaatc attgctgcgt
    12961 taccattttc cgtggtcatt atagcgatga tatggtcgct tataaaatct ttgagtgagg
    13021 agaaaccgag aaatagtaat aaggttttaa ttaaacatcg tgacccagat attttagaat
    13081 atcgttcttc aaaacatatt gacgaaaatt aattaataat gaggttttat ttacaaaaac
    13141 tagggaatat attactaaat ggtacttcat cttatatatt aaaaataaat ttaaagttat
    13201 taatttatta tgtttaagaa aaaaactgat gggtacatat ttaatatgtt tttaaattaa
    13261 ctttaactaa ggaggtgtcg ttaggggtga aacgacttaa aaactttatc cttggcttat
    13321 taatagtagt aatcgtgggg ttcctgttat tcatgtatat ccaagatgga cgtataactg
    13381 aatatcaaga ctacttcctt caatttgaat ggttccaacc attacttatt tcacttgctg
    13441 cactattgat attaataggt cttatattag tatttagtat cttcaaacct acgcatcgaa
    13501 aacctggatt atacaaagac ttcgatgatg gtcatgttta cgtatctcgt aaagctgttg
    13561 aaaagacggc attcgatacg gttgcaaaat atgatcaagt aagacaacca aatgtagttg
    13621 cgaaactata taacaaaaaa aataaatctt atatcgacat taaaactgac tttttcgtac
    13681 caaataatgt tcaagttcaa tctttaactg aagcaattcg ttcggatatt aaaaagaatg
    13741 tagaatattt cacagaaatg ccagtacgta agttagaagt taatgtgaga gatcagaaaa
    13801 cttctggtcc acgagtgttg taagggaggt atatcatggc taacaatcat aaccaaaacg
    13861 gacaagactc tacacaacaa gtgattaatt ttttaaaagt atttaaatgg agaatcgttg
    13921 gtttcctagc gtttctatta atcgcgatat tattcttaac gttaggtttt tggaaaacag
    13981 tacttatcat cgttttatgt ttaattggtg taggtattgg gtatatgaaa gaccgtaagc
    14041 aagattttat gaatttttta aatcgatgga gttaattcat caatttagaa tttaattaaa
    14101 aaaatataaa aattaacaag taaaaggaga atctattatg actgtagata acaataaagc
    14161 aaaacaagca tacgacaatc aaactggtgt taacgaaaaa gaaagagaag agcgtcaaaa
    14221 acaacaagaa caaaatcagg agcctcaatt caaaaacaaa ttaacattct ctgatgaagt
    14281 tgttgaaaaa attgctggta tcgctgcacg tgaagttaaa ggtatcttag atatgaaagg
    14341 tggcttaact gatacattca ctaatgcatt ctcaagtggc aacaatgtta ctcaaggtgt
    14401 atctgttgaa gttggtgaaa aacaagctgc tgtagactta aaagtaatct tagaatatgg
    14461 tgaatcagca ccaaaaatct tccgtaaagt aactgaatta gttaaagaac aagttaaata
    14521 tattactggt ttagatgtag ttgaagttaa catgcaagtt gacgatgtaa tgactcaaaa
    14581 agagtggaaa caaaaacacg aaaaaaataa cgaaaacaat aaccaagaaa gacaaggttt
    14641 acaataagtt attcgataaa aagggcttgg gacattaa
//
LOCUS       DAKTGS010000067        14635 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.067, whole genome shotgun sequence.
ACCESSION   DAKTGS010000067 DAKTGS010000000
VERSION     DAKTGS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14635
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.067"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            47..985
                     /gene="lukS-PV"
                     /locus_tag="O0286_001623"
     CDS             47..985
                     /gene="lukS-PV"
                     /locus_tag="O0286_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCY0170003.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            981..1964
                     /gene="lukF-PV"
                     /locus_tag="O0286_001624"
     CDS             981..1964
                     /gene="lukF-PV"
                     /locus_tag="O0286_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCY0170004.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            2213..2398
                     /locus_tag="O0286_001625"
     CDS             2213..2398
                     /locus_tag="O0286_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170005.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            2489..3406
                     /locus_tag="O0286_001626"
     CDS             2489..3406
                     /locus_tag="O0286_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0170006.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            3463..4401
                     /locus_tag="O0286_001627"
     CDS             3463..4401
                     /locus_tag="O0286_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0170007.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKY
                     DNLYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIH
                     KNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDM
                     PIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            4394..4600
                     /locus_tag="O0286_001628"
     CDS             4394..4600
                     /locus_tag="O0286_001628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170008.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            4615..5529
                     /locus_tag="O0286_001629"
     CDS             4615..5529
                     /locus_tag="O0286_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0170009.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            5728..5847
                     /locus_tag="O0286_001630"
     CDS             5728..5847
                     /locus_tag="O0286_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170010.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      5930..6064
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6126..6671
                     /locus_tag="O0286_001631"
     CDS             6126..6671
                     /locus_tag="O0286_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0170011.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(6777..7025)
                     /locus_tag="O0286_001632"
     CDS             complement(6777..7025)
                     /locus_tag="O0286_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCY0170012.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            7133..8086
                     /locus_tag="O0286_001633"
     CDS             7133..8086
                     /locus_tag="O0286_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0170013.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            8076..9455
                     /locus_tag="O0286_001634"
     CDS             8076..9455
                     /locus_tag="O0286_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCY0170014.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            9608..11068
                     /gene="ebpS"
                     /locus_tag="O0286_001635"
     CDS             9608..11068
                     /gene="ebpS"
                     /locus_tag="O0286_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCY0170015.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            11193..11309
                     /locus_tag="O0286_001636"
     CDS             11193..11309
                     /locus_tag="O0286_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170016.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            11470..12456
                     /locus_tag="O0286_001637"
     CDS             11470..12456
                     /locus_tag="O0286_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCY0170017.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(12571..13539)
                     /locus_tag="O0286_001638"
     CDS             complement(12571..13539)
                     /locus_tag="O0286_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCY0170018.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            13616..14275
                     /locus_tag="O0286_001639"
     CDS             13616..14275
                     /locus_tag="O0286_001639"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCY0170019.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            14306..14422
                     /locus_tag="O0286_001640"
     CDS             14306..14422
                     /locus_tag="O0286_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170020.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
BASE COUNT         5688 a         2116 c         2504 g         4327 t
ORIGIN      
        1 taatataaaa atgtaataaa atttataaag aaaggaaatg atttttatgg tcaaaaaaag
       61 actattagct gcaacattgt cgttaggaat aatcactcct attgctactt cgtttcatga
      121 atctaaagct gataacaata ttgagaatat tggtgatggc gctgaggtag tcaaaagaac
      181 agaagataca agtagcgata agtggggggt cacacaaaat attcagtttg attttgttaa
      241 agataaaaag tataacaaag acgctttgat tttaaaaatg caaggtttta tcaattcaaa
      301 gactacttat tacaattaca aaaacacaga tcatataaaa gcaatgaggt ggcctttcca
      361 atacaatatt ggtctcaaaa caaatgaccc caatgtagat ttaataaatt atctacctaa
      421 aaataaaata gattcagtaa atgttagtca aacattaggt tataacatag gtggtaattt
      481 taatagtggt ccatcaacag gaggtaatgg ttcatttaat tattcaaaaa caattagtta
      541 taatcaacaa aactatatca gtgaagtaga acgtcaaaat tcaaaaagtg ttcaatgggg
      601 aataaaagct aattcattta tcacatcatt aggtaaaatg tctggacatg atccaaattt
      661 atttgttgga tataaaccat atagtcaaaa tccgagagac tattttgttc cagacaatga
      721 attaccccca ttagtacaca gtggtttcaa tccttcattt attgcaactg tttctcatga
      781 aaaaggctca ggagatacaa gtgaatttga aataacgtat ggcagaaata tggatgttac
      841 tcatgctact agaagaacaa cacactatgg caatagttat ttagaaggat ctagaataca
      901 caacgcattt gtaaacagaa attacacagt taaatatgaa gtgaactgga aaactcatga
      961 aattaaagtg aaaggacata attgatatga aaaaaatagt caaatcatca gttgttacat
     1021 caattgcatt gcttttgcta tccaatacag ttgatgcagc tcaacatatc acacctgtaa
     1081 gtgagaaaaa ggttgatgat aaaattactt tgtacaaaac aactgcaaca tcagattccg
     1141 ataagttaaa aatttctcag attttaactt ttaattttat taaagataaa agttatgata
     1201 aagatacatt aatactcaaa gctgctggaa acatttattc tggctataca aagccaaatc
     1261 caaaagacac tattagttct caattttatt ggggttctaa gtacaacatt tcaattaatt
     1321 cagattctaa tgactcagta aacgttgtag attatgcacc taaaaatcaa aatgaagaat
     1381 ttcaagtaca acaaacggta ggttattctt atggtggaga tattaatatc tctaacggct
     1441 tatcaggtgg aggtaatggt tcaaaatctt tttcagagac aattaactat aaacaagaaa
     1501 gctatagaac tagcttagat aaaagaacta atttcaaaaa aattggttgg gatgttgaag
     1561 cacataaaat tatgaataat ggttggggac catatggcag agatagttat cattcaactt
     1621 atggtaatga aatgttttta ggctcaagac aaagcaactt aaatgctgga caaaacttct
     1681 tggaatatca caaaatgcca gtgttatcca gaggtaactt caatccagaa tttattggtg
     1741 tcctatctcg aaaacaaaac gctgcaaaaa aatcgaaaat tactgttact tatcaaagag
     1801 aaatggatag atatacaaac ttttggaatc aacttcactg gataggtaat aattataaag
     1861 atgaaaatag agcaactcat acatcaattt atgaagttga ttgggaaaat catacagtta
     1921 aattaataga tactcaatct aaggaaaaaa atcctatgag ctaaacagat agataatcaa
     1981 aaaatcttaa atatgttaaa atttacaaac actttctttc tatattaggg taaccacgtc
     2041 ttaattgacg tggttatttt ttcagggcaa aaaaagggcg gattatttaa ataaggacaa
     2101 acacttgtgg aaaatttaaa aggttaaaaa taataaagaa cttggtataa caagggtttt
     2161 atacatttgc gtacaacgac gaaatgtcaa tttaccatca cattatgacg atatgtttat
     2221 tttaaacaca caagctcatg cgcgtcttga tcaaatggca caacagtttg aagttgtttg
     2281 taatggcttg aacgaaaatg aaggacaagc aattcaaacg atggatcaat ctgcctctct
     2341 aatacggtca aacttaattc aagttaaaga acaattagaa aaactagctg tatactaatt
     2401 aatttattaa atgctacttg ttttcattga gaataagtag ctttttcaaa cataaaagtt
     2461 ttacaaacac ataaatgggt gatgagctat gtttaaaaga actaaactaa tcttaatagc
     2521 aacattactg ctatcaggat gttcaactac caataacgaa tctaataaag aaacaaaatc
     2581 agtgccagaa gaaatggaag cttcaaaata tgtaggtcaa ggcttccaac cacctgcaga
     2641 aaaagatgtg gttgaatttg cgaaaaagca taaagataaa attgccaaac gaggcgaaca
     2701 attttttatg gataatttcg gtctaaaagt taaagctacc aatgttgtag gtagtggaaa
     2761 aggcgtagaa gtattcgtgc attgtgatga ccacgatatc gtatttaatg cgagtattcc
     2821 atttgataaa tcaataattg agagtgatag ctcattaaga agtgaggaca aaggcgatga
     2881 tatgagtact ttagttggta cagtgttgag tggctttgaa tatcgaacac aaaaagaaaa
     2941 gtatgacaat ttatataaat ttttcaaaga taatgaagag aaataccaat atacaggctt
     3001 tacaaaagaa gcaataaaca agacgcaaaa tgtcggatat aaaaatgaat atttttatat
     3061 cacttactcg tcaagaagct taaaagaata tcgtaagtat tacgaaccat tgattcataa
     3121 aaatgataaa gaatttaaag aaggaatgga acaagccaga aaagaagtga attacgctgc
     3181 taatacagat acagtaacaa cattgtttag tacaaaggaa aattttacta aagacaatac
     3241 agttgatgat gtaattgaac tgagtgataa actatataat tttaaaaata agccagaaaa
     3301 atctacaatt acaattcaaa taggaaagcc tactattaat actaaaaaag ccttttatga
     3361 tgataatgat ccaatagaat atggagtgta tcgtaaagat gaataaattt ttttaaaatg
     3421 ctttttaata ttctatataa acacataaat gggtgatgag ctatgtttaa aaaggcgaaa
     3481 ttaatcttaa tagcaacgct actgttatca ggatgttcag cgatgaataa tgaatcaaaa
     3541 aaagacacaa atacagaaac aaatacagaa acaaaatcag taccagaaga aatggaagct
     3601 tcaaaatatg taggacaagg ctttcaacca cctgcagaaa aagatgcgat tgaatttgtg
     3661 aagaagcatc gtaaagaatt tgaaaaagta ggtgaacaat tctttaaaga taactttgga
     3721 ctaaaagtta aagctacaaa tgttgtaggt aaagatgatg gtgtagaagt ttatgtgcat
     3781 tgcgaagatc atggcattgt atttaatgca agtctacctt tgtacaaaga tgccatccat
     3841 caaaaaggat caatgcgtag taatgacaac ggtgatgata tgagtatgat ggtgggtaca
     3901 gtgctgagtg gctttgaata tcgagcgcaa aaagaaaagt atgataattt atataaattt
     3961 ttaaaagaaa atgaaaagca atatcaatat acaggtttta ctaaagaagc aattaacaag
     4021 acgcaaaacg tcgggtatca aaatgaatat ttttatatta catatttatc aagaaattta
     4081 aaagaatata gaaaatatta cgaaccattg atacataaaa atgataaaga gtttaaagaa
     4141 ggtatgcaac gagctagaaa agagctaaac tatactgcta atacaaatac tgtagcaacg
     4201 ttgtttagta cgaatgatga aaggaataga aaagaaaaga taaataatgt aatagattta
     4261 tccgagaaaa ttgaaagaac aaaagatatg ccaatcaaga atactataac tactcaatta
     4321 ggaaataaac ttattggcac aaaaaaagct cgttttgatg ataagaaagt agtgtcgttt
     4381 ggagcatttg aagatgaata aaataaatga tagagattta acagaattga gtagctatag
     4441 ggtttatcaa gacatcaata aagataatga ctttacagtt aacgaaaaac gatttaagca
     4501 ggcagatgta tttgaagatt tatatagaga gaaactaaaa gacacaaata aattaagaga
     4561 gtataattat ttacaaaatg aaacttttaa aagcgcataa ataggtgatg agatatgctt
     4621 aaaaaagcaa aatttatctt aatggcaacg atactactat caggatgttc aactaccaat
     4681 aacgaatcca acaaagaaac aaaatctgta ccagaagaaa tggatgcttc aaaatatgta
     4741 ggacaaggat tccaaccacc tgcagaaaaa gatgcgattg aatttgcaaa gaagcataaa
     4801 gataaaattg ctaagcgagg cgaacaattt tttatggata acttcggtct aaaagttaaa
     4861 gctacaaatg ttataggtag tggcgatggt gtagaagtat tcgtgcattg tgatgaccac
     4921 gacatcgtat ttaatgcgag tattccattt gataaatcaa ttattgacag tgatagctca
     4981 ttaagaagta aggataaagg tgatgatatg agtactttag ttggtgcagt actcagtggg
     5041 tttgaatatc gagcacaaaa agaaaaatat gataaattat ataaattttt caaagataat
     5101 gaagagaaat atcaatatac aggatttaca aaagaagcaa ttaataagac gcaaaatagt
     5161 ggttatgaaa atgaatattt ttatatttcg gccatacctt ataatttagc tgagtataga
     5221 gactattttg aaccattgtt aaacaaaagt gacagtgaat tttcaaaaga attgtcaaat
     5281 gttaagaagc aattaaaaga taagtctaaa gtttcggtaa ctactactct atttagtaaa
     5341 aaaaagaact atactaaaaa aagtaacagt gaaaatgtaa taaaaatggc agaagaaata
     5401 aaaaaagata aagagatacc aaacggtata gagcttagta taaaattttc ggacaataaa
     5461 ataaatacgg ttaaaccaaa ttttaacggt gaaagcactt cagaatatgg tgtgtttgat
     5521 caagaataaa attaatgatg aaaatttaac ggagaatagt gtatattgag tagatcaaga
     5581 ataaaaagat aattctacta ttgttgtgaa ggcaaataag tagaagattt taagtgtaat
     5641 ttctggtgat ttaaataata atataaatgg aagtactgat ataaaacttt ttaacctact
     5701 agattcttat aatttgcttt ccattttatg acgattttta ctcaattgag tgatagaatc
     5761 aaaaaagcca tctcaaaaat taatcaagca aacaacattc caaacaatgc tcgcaaatca
     5821 ccaatgtatc actctccaat tacgtaacta tgatttaatt taagcatagt tattgaggtt
     5881 ttgtgatata tagtataaaa ttaatgagaa ttaaatttaa taatgtaaaa ttcatcttcg
     5941 gggtcgggtg taattcccaa ccggcagtaa ataaagcctg cgacctgcta gtatgtatca
     6001 tattagtggc tgatctagtg agattctaga gccgacagta tagtctggat gggagaagat
     6061 ggaggttttt tgttgtgcaa taatcctcct attcttacga gatgaatgga aggagaaaat
     6121 tgaatatgca acaaaataaa cgtcttatca caataagtat gttgagcgcg attgcgtttg
     6181 tgttaacttt tatcaagttt cctataccat ttttgccacc atacttaact ttagatttta
     6241 gtgatgtacc gtcactacta gctacattta cgtttggacc agttgccggt atcgtagttg
     6301 cactggttaa aaatttattg aactacttat ttagtatggg cgatccagtt ggaccatttg
     6361 ctaacttttt agcaggcgca agtttcttat taactgctta cgccatctat aaaaataaac
     6421 gttcaacaaa atctttgatt actggattaa tcattgcaac aatcgttatg actatcgtgt
     6481 tgagtatttt gaactatttc gttctattac ctttgtacgg tatgatattt aacttagctg
     6541 atatcgcaaa taatcttaaa gtaatcattg tttcaggaat tataccattc aatattatta
     6601 aaggtatcgt tatttctatt gtatttattt tactatatag aaggcttgcg aatttcttga
     6661 aaagaattta atcaaattaa agcaaaataa tatacacata ataataaaaa gcaggtgact
     6721 atcaataaac gatagcttgc ctgctttttc tatagaacat ttgtctaaaa aatcaattat
     6781 tcaaatttta aagcgtcccc atcaaatgat tcgtctgcaa ttttaataga atctgtaggg
     6841 catccatcaa ttgcatcttc catatcttca tataattcct caggtacttc tgcagtacct
     6901 tggttatcgt caaggattac gaaagcaata ccttcgtcgt cgtaatcata tatatctggc
     6961 gctgctgcac cgcatgcacc acatgcaata caagtatcca tatcaacgat tgtatatttt
     7021 gccaatgtct tcgcctcctt tgataaaaat gctaaaatag taatgtgact aaaattttag
     7081 acagcatcat ttttattttc aaattatccg ttttacagag tgagggttaa atttgcaaca
     7141 cattataaaa acagcattac aacaaacatt taactataaa acaaataaaa gtatttacaa
     7201 tatcttagtt ggtaagaaat ctcaccaaac cttttttgac gcttgtagtc aacaacagtt
     7261 gtcattatat cacagtttac cactattaaa atatccgtct tttgagctat ttttagaaaa
     7321 aatcaatgaa tttaatgctg aaatggaaat catgttgcat cctagatata catttgaaag
     7381 catggggcaa acatttcaag caattcaact attagtgcaa accatgtcta ataccaaaca
     7441 acatgttttt cattttgtac caatctctca aaataataag atacaagaaa ctgtaaagat
     7501 tgtctataac tacattaaag aaaaccaatt acaaattgat tttgaaaatg aactacataa
     7561 cttgtttaaa gcaataactt taaaagggcc atgttactta cattattatt tgcaaggcta
     7621 tgatgaacca atgtatacga gacagcaagt tagtttaata gaaaagctat ctcaacagca
     7681 attgtttgaa tacgaaatga ataatttagt gacaatgatg tttgaattag aaagtggaga
     7741 atatactatt ttatcaaaaa taataatgaa acctacatta ttaaatcaaa cttatattac
     7801 ttatacaaaa ttgcttgaac aattcacgat ggaagatata gcggctcaac aacaagttaa
     7861 aatcaatact atcgaagatc atgtacttga aatcttaatc aaaggttaca tgtctaatta
     7921 cgatgattat gttgaactag aagatcaact ccagtttttg aatttttatc aacagcatcg
     7981 tggcgaacga ttaaaatttt acaaagaaca atttgacacg ttatcatatt ttcaattaaa
     8041 agtattaatc gttggatttg aaagaggtga tctgaatgtt gcatgatatt ttacgaaaca
     8101 aatttggatt cgagagtttt aaaccgggac aacaggaaat tatagaaagt ataatgtctc
     8161 aacaacacac tctaggtata cttccaactg gaagtggaaa gagtttgtgt tatcaaatac
     8221 ctacgtattt atcaggtaag ccgacattaa ttatctcacc gttaatatct ttaatggatg
     8281 accaagttat gcagttgaaa ataaatggag aaaaacgtgt aacatgtatt cactctggta
     8341 tggatgaaat tgagaaaaag cataatatta aatgtttacg acatagccgc ttcatctttc
     8401 taagtccaga atttctcctg caaccgtcaa attttaaatt aatatctatg atagactttg
     8461 gcatgattgt tctagatgaa gcacattgcc tatctgaatg gggatatgat ttcagaccac
     8521 attatgctct aataggaaaa gtaacaaagc attttaaaga agcggttgtc ttagcattga
     8581 cagcaactgc accaccgcat ttacaagatg atttgacgga aatgttagcg attcaattca
     8641 atgttattaa aactacaatg aatcgcccaa atataagctt taagcatctt aattttcatg
     8701 atgatgaaga taaaattgaa tggttgctgc cgtttctaca acagtcggga ccaacgatta
     8761 tttatgtctc atcgaaaaag atgtgtctga atttagcgca acttatttat gattcaggtt
     8821 ttcttacagg tatttatcat ggtgatatga attatcaaga gcgacacaca gttcaacaac
     8881 aatttttaaa taatgatatt ccgattatag tcgcaacgag tgcttttggt atgggaatta
     8941 ataaaaaaga tattcgcaca atcattcact ttcatctttc aacaagtcct tctaactaca
     9001 ttcaagaaat tggccgtgcg ggtcgcgatg gtgaactaag tcaggcaatt agtttattcc
     9061 aaccggacga taaatatatt ttagaaacgt tattatttgc agatatgata acagaagaag
     9121 atgtacaaaa tttcgaaata ggagaatttt tagctcccga taaacaagcc gttttgacaa
     9181 cgttgcaatc attctatagt atcggcgcct tgaaacagat atttaagcaa tcatttaaac
     9241 gaaagcaatt aggattcttt cgcatgattg gctattgcaa attggatcaa tgtagacgga
     9301 agtatttatt agaatttttc ggtgaatatc caccggcaca agatcgatgt tgtgacaatg
     9361 attctaatat aactgatatc gcaattttaa ataagaagaa ggtaattaga agtattggat
     9421 ttgatgaaaa gttgcaaaat ttatttctca gatagtatta ctttactaaa agaaaattga
     9481 caagctataa ttagtgtata cacaattgaa aaatgattga aataattttg aaaaatatac
     9541 ataaacatat gtcatgtggg tatattttat gtaaaatcat tgtaatagaa tagaaaggaa
     9601 gatggctatg tctaataatt ttaaagatga ctttgaaaaa aatcgtcaat cgatagacac
     9661 aaattcacat caagaccata cggaagatgt tgaaaaagac caatcagaat tagaacatca
     9721 ggatacaata gagaatacgg agcaacagtt tccgccaaga aatgcccaaa gaagaaaaag
     9781 acgccgtgat ttagcaacga atcataataa acaagttcac aatgaatcac aaacatctga
     9841 agacaatgtt caaaatgagg ctggcacaat agatgatcgt caagtcgaat catcacacag
     9901 tactgaaagt caagaaccta gccatcaaga cagtacacct caacatgaag aggaatatta
     9961 taataagaat gcttttgcaa tggataaatc acatccagaa ccaatcgaag acaatgataa
    10021 acacgatact attaaaaatg cagaaaataa cactgagcat tcaacagttt ctgataagag
    10081 tgaagctgaa caatctcagc aacctaaacc atattttaca acaggtgcta accaatcaga
    10141 aacatcaaaa aatgaacatg ataatgattc tgtaaaacaa gatcaagatg aacctaaaga
    10201 acatcataat ggtaaaaaag cagcagctat tggtgctgga acagcaggtg ttgcaggtgc
    10261 agctggtgca atggctgctt ctaaagctaa gaaacattca aatgacgctc aaaacaaaag
    10321 taattctggc aaggcgaata actcgactga ggataaagcg tctcaagata agtctaaaga
    10381 tcatcataat ggcaaaaaag gtgcagcgat cggtgctgga acagcaggtt tggctggagg
    10441 cgcagcaagt aaaagtgctt ctgccgcttc aaaaccacat gcctctaata atgcaagcca
    10501 aaaccatgat gaacatgaca atcatgacag agataaagaa cgtaaaaaag gtggcatggc
    10561 caaagtattg ttaccattaa ttgcagctgt actaattatc ggtgcattag cgatatttgg
    10621 aggcatggca ttaaacaatc ataataatgg tacaaaagaa aataaaatcg cgaatacaaa
    10681 taaaaataat gctgatgaaa gtaaagacaa agacacatct aaagacgctt ctaaagataa
    10741 atcaaaatct acagacagtg ataaatcaaa agaggatcaa gacaaagcga ctaaagatga
    10801 atctgataat gatcaaaaca acgctaatca agcgaacaat caagcacaaa ataatcaaaa
    10861 tcaacaacaa gctaatcaaa atcaacaaca gcaacaacaa cgtcaaggtg gtggccaaag
    10921 acatacagtg aatggtcaag aaaacttata ccgtatcgca attcaatact acggttcagg
    10981 ttcaccggaa aatgttgaaa aaattagacg tgccaatggt ttaagtggta acaatattag
    11041 aaacggtcaa caaatcgtta ttccataata taactatata aattgtaact gaactgctat
    11101 atacaaacgt gcatattata agtatctcta aatattttgg agaacttaaa ttgcgcttat
    11161 aagtatgtag cggttttttc atttttcaaa gtttgttatt taacaaggtc ttttctcgaa
    11221 tattggcata tcaatttaac tttttaaata gtcatcaaaa agataaaaca ccacaatcaa
    11281 caaatttaac gaggaagaat aaaaaataaa tcaacatatt aaattgtagt gttattcaac
    11341 tccgtagcta acaattctct atgcacatta aacaaattgt caaaaatata tcataaatct
    11401 tcaagcacag acttagcgca tcaatcactg aactgttata atagtttggg attaaaggag
    11461 gccgaaacaa tgcaaaaagt tgaaagtatc ataattggtg gagggccatg cggattaagt
    11521 gcggctattg aacaaaaaag aaaaggtatt gataccttaa ttattgaaaa gggtaatgtc
    11581 gttgaatcaa tctacaatta tcctactcac caaacatttt tctcatcaag tgataaatta
    11641 agtattgggg acgtaccgtt tatcgttgaa gaaagtaaac caagacgtaa tcaagcgcta
    11701 gtttattacc gagaagttgt aaaacatcat caattaaaag taaatgcatt tgaagaagta
    11761 ttaactgtta aaaaaatgaa taataaattt actattacta cgacgaaaga tgtttatgaa
    11821 tgtcgatttt taacaatcgc gacaggctat tatggtcagc ataatacatt agaagttgaa
    11881 ggtgcggatt tacctaaagt gttccattat tttaaagagg cacatccgta ttttgatcaa
    11941 gatgttgtaa ttatcggtgg taagaattcg gctatcgatg ctgctttgga gttggaaaaa
    12001 gctggtgcta acgtgacggt tctatatcgt ggtggagatt attcgccttc aattaaaccg
    12061 tggatacttc caaatttcac agcattagta aatcatgaaa aaattgacat ggaatttaat
    12121 gctaatgtta cccaaataac tgaagatact gtgacttatg aagtaaatgg tgaaagtaaa
    12181 acgatacaca atgattatgt atttgcgatg attggttatc atcccgatta tgaattttta
    12241 aaatctgtag gcattcaaat taatacaaat gaatttggaa cagcgcctat gtataataaa
    12301 gaaacatacg aaacaaatat cgaaaattgc tatattgcag gtgtaattgc tgcagggaac
    12361 gatgcgaata ccatttttat tgaaaatggt aaattccacg ggggcattat tgctcaaagc
    12421 atgctagcta agaaacaaac gcccttagaa tcataaaaat aaaggtctat gtactaaata
    12481 acttagtttt acaacgactg acattcatga tatgtcaaat gaggttgatg actaatgatt
    12541 gtacatagac ctttttatgt tacgtattca ttataattca aaatatgatt tgatttcagc
    12601 tttatctaaa ttgttgctta acgcgactaa taattttaat cttgcttttg gaccattcaa
    12661 gccgttagaa aaaataaaac cttgttgtgc gagttggtaa ccaccaccat cgtatgcgta
    12721 agttggactc acaataccat taaaggaacg tgaaactagc acaataggta tatttaaaga
    12781 tactaattgt tgaatgcctt ctaatgcgct tggaggtatg ttgccttgtc ctaacgcttc
    12841 aataaccata ccatcgatac cttctcgact ataaaaacta aaaatgtcac ctggcatacc
    12901 catatatgct tttactaatg gtacatttag tttgtcattg acattttcca atgcttgttg
    12961 gcgatatggc atatgatgga attgcacacg atcctttgtc aatacaccta gcggaccatg
    13021 atttggactt tgaaatgtgt ttgtattaga cgtatgtgtt ttggtaacat tacgcgccgt
    13081 atgaatttca tcattaaata caaccatcac gcctttatga cgggcctttt catcagaggc
    13141 aacgcgaata gcggaaatat aattatataa tccgtcagaa ccaatttcat tagacgagcg
    13201 cattgcgcca gtaataacaa caggttgctc gatacctaat attaaatcaa gtaaaaaggc
    13261 agtttcttct aacgtatcgg taccatgcgt gataacgaaa ccatcataat atttatttgt
    13321 tactgcttct aaaataatat cctttaattg tttaacatgt tggattgtca tatgaggtga
    13381 tggtacatta aaaggattta attcatctat ttgtgcatat tgatttatga catcttgatg
    13441 cattgaaata gggttgatat catttgttac tactttatta gattggtctt gtgacatact
    13501 aatggtgcca ccagtatgaa taacaagtag atgtttcata tatttcctcc tatatttaat
    13561 ttacctaatt atgataaaat attattcata aaacgacaag gaagggaaat gacgcatgaa
    13621 agccattaat attgcattag atggtccagc tgctgccgga aaaagtacaa ttgcgaaacg
    13681 tgtagccagc gaactatcaa tgatttatgt cgatacagga gcaatgtatc gtgcattaac
    13741 atacaaatat ttaaaattaa acaaaactga ggactttgca aaactagttg accaaacaac
    13801 attagattta acttataaag cagataaagg tcaatgtgtc attttagata acgaagatgt
    13861 aacagacttt ttaagaaata atgatgtgac gcaacatgtt tcatacgttg catctaaaga
    13921 gccagtacgt tcattcgccg ttaaaaaaca aaaagagtta gctgcagaaa aaggtatcgt
    13981 aatggatggt cgcgatatcg gaactgtagt gctaccagat gcagatttaa aagtatatat
    14041 gattgcatca gttgaagagc gagcagaaag aagatataaa gataatcaat taagaggtat
    14101 cgaatcaaat tttgaagatt taaaacgtga tattgaagct cgtgatcaat atgacatgaa
    14161 ccgtgaaata tcaccattaa gaaaagcaga tgatgcagtg acattagata cgacaggcaa
    14221 gtcgattgaa gaagttactg acgaaatttt agcgatggtg agtcaaatta aataatctaa
    14281 taagtcataa ctatctattt gtaatgtgca acgcttaacg catatacaaa atgaatgtgc
    14341 tgataatgat ttactcaaat taaaaggtga tttttattca atgatgaatg aaagttgcct
    14401 ttttattttt ggtaaaagtt aatgcgtcag tgaattgtgt aagtttttca aaaagtaaaa
    14461 agaaataata aaggtgaatt attagaattc caaaaataat tcattacatt cataaagcat
    14521 tttacaaatg gtaagaaaat gagtgttaca aatctaaata ttgcaaaaga agctgattta
    14581 gtcacaaaaa atgtcctatg taataattcg agaaagatgc actatatacg gtctt
//
LOCUS       DAKTGS010000068        14621 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.068, whole genome shotgun sequence.
ACCESSION   DAKTGS010000068 DAKTGS010000000
VERSION     DAKTGS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.068"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..183)
                     /locus_tag="O0286_001641"
     CDS             complement(<1..183)
                     /locus_tag="O0286_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0170021.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYK"
     gene            complement(460..612)
                     /locus_tag="O0286_001642"
                     /pseudo
     CDS             complement(460..612)
                     /locus_tag="O0286_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(612..1103)
                     /locus_tag="O0286_001643"
     CDS             complement(612..1103)
                     /locus_tag="O0286_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0170022.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            complement(1287..2345)
                     /locus_tag="O0286_001644"
     CDS             complement(1287..2345)
                     /locus_tag="O0286_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCY0170023.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            complement(2332..2538)
                     /locus_tag="O0286_001645"
     CDS             complement(2332..2538)
                     /locus_tag="O0286_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170024.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(2763..3107)
                     /locus_tag="O0286_001646"
     CDS             complement(2763..3107)
                     /locus_tag="O0286_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0170025.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            complement(3778..6150)
                     /locus_tag="O0286_001647"
     CDS             complement(3778..6150)
                     /locus_tag="O0286_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0170026.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(6242..6490)
                     /locus_tag="O0286_001648"
     CDS             complement(6242..6490)
                     /locus_tag="O0286_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCY0170027.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(6491..6874)
                     /locus_tag="O0286_001649"
     CDS             complement(6491..6874)
                     /locus_tag="O0286_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0170028.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(6876..7079)
                     /locus_tag="O0286_001650"
     CDS             complement(6876..7079)
                     /locus_tag="O0286_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0170029.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(7046..7219)
                     /locus_tag="O0286_001651"
     CDS             complement(7046..7219)
                     /locus_tag="O0286_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0170030.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(7231..7503)
                     /locus_tag="O0286_001652"
     CDS             complement(7231..7503)
                     /locus_tag="O0286_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCY0170031.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(7496..7759)
                     /locus_tag="O0286_001653"
     CDS             complement(7496..7759)
                     /locus_tag="O0286_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0170032.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            7952..8284
                     /locus_tag="O0286_001654"
     CDS             7952..8284
                     /locus_tag="O0286_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0170033.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            8296..8754
                     /locus_tag="O0286_001655"
     CDS             8296..8754
                     /locus_tag="O0286_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0170034.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            8771..9499
                     /gene="seq"
                     /locus_tag="O0286_001656"
     CDS             8771..9499
                     /gene="seq"
                     /locus_tag="O0286_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HCY0170035.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
     gene            9523..10251
                     /gene="sek"
                     /locus_tag="O0286_001657"
     CDS             9523..10251
                     /gene="sek"
                     /locus_tag="O0286_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HCY0170036.1"
                     /translation="MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDL
                     KDVKDNDTPIANQLQFSNVSYDLISESKDFNKFSNFKGKKLDVFGISYNGQCNNKYIY
                     GGVTATNEYLDKPRNIPINIWINGNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEY
                     NIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKI
                     YEDNKTVESEKFHLDVDISYKETI"
     gene            10339..11559
                     /locus_tag="O0286_001658"
     CDS             10339..11559
                     /locus_tag="O0286_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0170037.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(11702..12523)
                     /locus_tag="O0286_001659"
     CDS             complement(11702..12523)
                     /locus_tag="O0286_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0170038.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(12541..13236)
                     /locus_tag="O0286_001660"
     CDS             complement(12541..13236)
                     /locus_tag="O0286_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170039.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(13229..14254)
                     /locus_tag="O0286_001661"
     CDS             complement(13229..14254)
                     /locus_tag="O0286_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0170040.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(14343..14446)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(14504..>14621)
                     /locus_tag="O0286_001662"
     CDS             complement(14504..>14621)
                     /locus_tag="O0286_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCY0170041.1"
                     /translation="CKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
BASE COUNT         4767 a         2535 c         1992 g         5327 t
ORIGIN      
        1 cttatattta tcaggaacca aaaaattacc attaacatct agttctaaaa ataaatcttt
       61 ataaccattt atgatattat aataattttc attttctaaa tctcttttag cactactcgg
      121 tacttccatt cctcttcttc ttagaatttt caattgttta ttatgacttt caaatggctt
      181 cataaattta cccctataaa agtagccata acccgaatag agttatggct agatcgttat
      241 atatataata catttaattt tatcggttgt aaataaataa gaaataacaa aaagagatat
      301 tttacacaat tacctccttt attttattgc tactcctcaa acccaccaat attatcaata
      361 aacactggtg ttgttacatt taagtctact ttctcagtaa ataagctatg gtatcaaact
      421 aattagtatt aatttataaa tagcatagct tcattttctt caatcctcta acggtatatc
      481 atccacaatc acagtatgat tatggttagc gttagataca tcttgcactg ccttatctaa
      541 ttcctcatca tctccgtccc attcaccaat attaatgaat ataggaatat tcccgttaat
      601 atcatgctta tctatgaaag ttctttttta acttgttcta aatgcttatt aaactctatt
      661 cgatgtaatt cttctcgctg gttttgtcgt tcttgacgct ttctactttc aactttataa
      721 atatctactt ttaaacgttc aatgactcct tcatttttaa ctttcccact acttaacatg
      781 tacctcataa catcctgttg ttcattttca gaatagtttt tgatgatatt tttaaacaaa
      841 tttagcctat cctgagctgt tttcatgtaa tattcaagtt tatctttctc ttctataata
      901 taaagtacta atttatctaa tggatatgat acagatacca gcatatcgtt atcattcgtg
      961 atcacatggt tcatgttcaa gtgatataag ctttctattt gtgatctcat cgctttaatt
     1021 ttgctattaa tgactttcgg attgtaatgc attaaaagtt ctacttcagt gattttatca
     1081 ttgctttcaa agtcagatac caaataacca cacctttaca tatcgaattt aatatttata
     1141 agtatctgaa aaagggataa atcaagttat ccccctttca taaaagttta tccgcgttgc
     1201 aagcgaaggg cccccgccgg tacccggcga aaaaacattt taagccgatg ggcagggggc
     1261 tataaaattt tatttaaata atttttttat ttaatattaa ctatgcgcaa taactctgga
     1321 attattttta tatcaaacac atagttcaat gcaaattgat aaataccttt taatgcttgt
     1381 ttctttttgt tatacataat aggttcgtat ttagcatata tggcatgtcc aaagtctcca
     1441 acaaagacat catgtgcatc atcagtaacc actacattag tcacattaaa taggttactt
     1501 aaatcacctg caagtccacc taatcccatc tgtgccaact ctttgatgag cttatcatga
     1561 tgttctgtgt tcatcacaat agatacatta cgcctaaacg tttgtggtat atctttcatc
     1621 gcttgcttga taccatcgta tattgatgtg ttacttactt ctttgatacc ttgattataa
     1681 agtgataagt cttggttacc tttatcaatt aaaccacttg aattattctt atctaataac
     1741 aatgttgttt catatgcgac acactcatta ataaatatct ctttaagtag atcatctaat
     1801 gttctatcag agccatgaac tacactgtct gatacttctg tgtagatatg tccctcacgt
     1861 ctatcaaagt atttactact gccattaagt gatagctcaa taccgttagt agctaactta
     1921 tctttaatgt atgttagtga tggtatagat tcaacatgtt catcatcagt aatggtaatc
     1981 acctcacgta gtgattgagg tagctcacaa ttcaccaata actctttaaa tacttcttta
     2041 cgtgcagttg gtaactcttg aatattaaag ttattctcac tactaactaa ttgttttgtt
     2101 aaggtaccta cccatgtatt acttgattga tttacttcat tcatatttaa cactcctaat
     2161 aattttttgt taatttgctc tggtaataaa acttctgttt cagtaatact taattgcttt
     2221 aacccctgtt caatcatact tacatcttta cctatatcct tttgatatag attggcatat
     2281 cctttaatat ccatatcatc aaagttagat agttgatacc caaattgttc tttaaactct
     2341 ttcataatct tcatctccta atttgttttt agtatgaaca actaaaccat atatacgttc
     2401 gttgttttta tcttcttcat agaacgaaat gttatgaaga aagtatttgt ctaataattc
     2461 gtgatactta tttaatagtt cttgcttatc tctatactta aacacacgtt ttacagattc
     2521 atttgtatga tctagcacgt cttgcctctc tccttttaat ctgatagata tacattttaa
     2581 taagttcaaa atcagcacgc ttttttgtat agcgccttac aacgcgattt atatatttcg
     2641 tattcattaa ttgaacgttc acaggtgtat gtttgattat ttgatatgcg attcttttat
     2701 gattaataaa tatacacact cccatatcta tacttgatat tttaaatgct tatctgcatg
     2761 ttttaattta ctaaactaaa tgttactggt agccaatgat ctatttttat atttttattc
     2821 tttacaattg gtagccttag acctttacca tcaataagat agtacattgg cggacaaatt
     2881 tgcatttcaa ttagcccgtc gcgttttaaa tctgccacaa tgttaaacgc ttcttcattc
     2941 caacccatcc aaaatataat attcttatgg ttgccactgg tatatgcgcc gttacctttg
     3001 tatttaaaac cttgttcttc aaatacattc tcaatctcta caaatgaagt gctgtcattg
     3061 ttctttatat attctaagat gatttgtttc attctatttc tattcatgtt actctagtcc
     3121 tccaatctat ccagttacta aagcaaccac gttagatact aaaaaccact gaatttatgt
     3181 ttttcaataa ccactgtaaa cacagtgatg tcaatacata caggctgttg gttactgagt
     3241 tactgaaaat tcacggtatt ttatataatc ttttattacc ttaattctct attatacatt
     3301 tctactaaaa agaaataatt tatagtaact cagtaacttt ttgtcaaaaa gactaatatg
     3361 aaagtaaaaa ctgtggttac tgaattctag aattttagta tcttttagtg acttaaacgt
     3421 tttttagtaa ctttagtatc ttaggcaatt ttgtataact gcaagtctct tccgattatg
     3481 tgcaactgaa atttacagct atgcaactcg ttttaaattt tccagttgca atttttttct
     3541 tactgtcaca aggggttaat ccacttttgc aactatgcaa ccgaaatcac acaaattagt
     3601 ttatataatt tttgatacta tttcttattt atattttggt tatttacttt tctactaata
     3661 agttatagat tttgagttgc tcagttgcat aagtgcccta aacccttgat ataactgtga
     3721 ttataggtgc aacccgtttt aaaattttcg gttgcattcg gttgcattcg gttgcaacta
     3781 tattgctttt agcttcacct tttcatacga tttatagttt tttccacttt caataggttc
     3841 aatgtaattt ctattgtcat taaagttgta tatcctctta gcaagttctt cattgtcata
     3901 tcttcgctgt gcatcagttt tccaataatt ctctaaataa tgttcaaatt gtttataaaa
     3961 cttgtttgaa ctcaatgcat tatagccatt ttcatcacaa tattctttat aaaatgcgta
     4021 tacaatatat ttcggcactt ttctaatagt ccattgatca aacatattta ctttaaaccc
     4081 ataaactgga tcgttatctt ctttaaatac ttcaagcatt tttttagatg catcaggaat
     4141 gtcaaaggtt tcaaaatcta aattaattgc tttataaagc acatactcta gcacttgctg
     4201 attttttata tagtcttctt tgattttaaa attctcttta atgccattaa aattggcatt
     4261 aaacggtact atcaataacc ttcttaaagt cccgcctgtt ttgtctttaa atttaggcat
     4321 tccattcgtt gattgaataa ctgtacactt aaaagtcgct ctatataagg gtttattttt
     4381 aaactcaacc aacactggat cgccagtaac tacactttta aagtttgaag aatcatcgac
     4441 atacacacca actggtacat cgtctccaat tactgctgtc ttgccctcta acacactcaa
     4501 tttaaaacgt tcatcaaact cattcacttt taagctagca atattgctat aacctattac
     4561 attagacaat aattcttgaa acgtcccttt accattattt ccatcaccaa caaagaaaat
     4621 agccttttta cgagtgtagt taccattcat tgagtcatta attacttgcc acaataattt
     4681 aacaacctga ttatcattac aagcaatctc ttctatccac cgatctatat tccagccatt
     4741 tatttcaggt actatatctt gccttacata cgatgtatct attttactgg taaatatata
     4801 atcaggtgta aatgattcta gttgcttcgt tttacggtta aatacaccat ttttaactgg
     4861 tattaagtat ggtgagttag ttttctcttt tatatctacc atattggtta aatgataaat
     4921 aacttcatca gctttgttgc tattatgttt gggctctaaa taggaaatca ctcgttttat
     4981 aattgtagtg ttctgtgtat atatcccttc atcaaattga tacatagcta acttagtatt
     5041 ttcttcatca tcaaaaagta taaatgttaa atgctcattc aaaatatatg cacattttat
     5101 agggctaatt gttgtaggct ttcgccctcg ttttccttct tcaatccaat tttcttccat
     5161 ttttgttctt tcttcatgcc ccaattgatt caacaactgt ttaacatctt tactcccctg
     5221 tggtataaca ttaaattcat ctatcgattt agtataaatt gtcttttgat tctttatagc
     5281 tttatctaac tcaagctgtc cccaagtcgt attacctcgt ttactttccc atttatccgt
     5341 aagattattg tagtttaaaa atatacgctc catttgctgt ttgtttttac ctgtgtaaaa
     5401 cgctaagtag tgtaataagc tttgtactgc ttcgcttgaa ctctcaaaat atgattcata
     5461 tgtgccttgt aatagatctt taattttatc tttttgttta gatttcatca taatgtttat
     5521 aatatcttca tcagataatt gtatattggt attagattcc tctctcacaa cctcatttgc
     5581 tggcaaattc tctttaaaat attcatcaac tagagtattt aatacttctt gattatcaca
     5641 aatatcactt tgaccaattg tgcatcctgt aacagtcata aaccttgctg aatcatataa
     5701 ctctatgtct aaatctgttc ttttcttttt acggttatct ggtagtttac ctttaaagaa
     5761 acaatgtaat cctgtaccac ttggagactt ttcacaatat gtgagttgca tcatttttag
     5821 tgctaattca gaattgattt gtcctttttt atcaattgca ttatcaatat ctaggcaaac
     5881 aaaattatta ccatcactta atacaaaacc tataccgcta tattgctcat caacttcata
     5941 ttcaatactt acactttcaa agtcacacca tgttttttta ttggttgtac tagctctgta
     6001 accattaata ctatatggta cttttccaaa attttgttgc ttctcgttcc attcagctcg
     6061 ccataatacc caattgggca ctaattttaa ttcttgcggt atttctaatt ctttaactcc
     6121 aataattcta tcttttttct taattgccac agaatgcctc ctttctaatc tgatttctct
     6181 gtattttcaa tgcaactaat gttaaaatat taatggtatg attttataaa tttttatttt
     6241 tttatgcgtt atcttggctt tggtcggcga aagatgacgc tttttctaat gcatcaaaca
     6301 tttgatcatt gtgaatacgt ctctcatgat atgaaaatcc ttctttaata gcatcgtctt
     6361 tatttaaggt atgatttggc tcatgtagat ataaatcttc aaaatgcgag ccatgattat
     6421 cctttaaatc ctcaaacttt tctttcaata tctttaaatc acacattaaa tttttaattt
     6481 cccaattcat ttataattcc tccacttcaa tatttcccac aatgtaatct aatgcccact
     6541 ctaacattcc aatcacgtgt cctttgcgat ctgttgtatg tttatgttca cctttttcgt
     6601 ctatgacact ataacggtaa acgtgttgtg tttctttcat gacatcactt agcgtcattg
     6661 ttacttcatc aagaattaaa aatgcttcat cttcaaaatc taattcagca agaatattga
     6721 acaatccatt atgaactaac tttaaagcat attcatataa tgctttatcc tgataatgat
     6781 agtccttatc ggattgaaac ttatcttcct cgtataatac tacttctaat attttaacca
     6841 cttttgataa ttgatatttt tgtttgattt ccatcttaac tacctacttt tttcttattt
     6901 ttaatttctg taatttttga taaatccatt tctaaacacg caacttgaac atctttacta
     6961 acatcaggaa agtactgttc aaatactttt ggcggtatat ttaacattaa attatgttga
     7021 gtatctttaa tgttgtacca gccaactact gtttttgtaa taataacttg ttttcgcacg
     7081 ttgtaatctc tcctgttaaa ttaaatccat aagttaccat catgccgtac acactaaaag
     7141 cgacatacat gttagatatt gctagtaata atattgttaa caatgaaact aagcagatat
     7201 aagttaagta cattttcatt gccttgcctc ctacatccat tttttatgac gcgccttcat
     7261 gtactcctcg aatcgcggaa tactgataac aatcattgtt gatgataacg agtaatataa
     7321 atcatcaaca cctttagaat ctttttccca ctcttttaaa atgcgattca ccgaactata
     7381 tgaaattccc aaaatactag ctagtgcatt aggtttcgca aacaaaggat tcactacaac
     7441 ttgctttggt tctgtaattg tattttcttt agctggtaca tcatgcaatt ttgttctaga
     7501 catttaattc acctcctttt aagatgtttt ttcgttcttt tccgggaacg ttattggtaa
     7561 aaaaaatgtc taggttattt gtttcataac ctaatatttt tgccatttta ataaattcat
     7621 tcgctccaat atctactatt ccattttctc tctttgcata aggcgttctt gttttccacc
     7681 ccattttgtg agccatttca tcttgcgtaa taccacaagc tattctttcc gctctcaatc
     7741 tttttaaatt tagtaccacg tttacacctc ctatcgttct cgttcgagaa ctaattacaa
     7801 tttaccattt acgttctcgt tcgtcaacaa tttttactta aaaaaattca aaaagttttt
     7861 tcttccttat atattgtatt cttttgggaa cggtgttata atcaaatcgt tcacaaataa
     7921 gaacaaatat tcaattcagg agatacaaga aatgagaact aatgatgaaa taatcacaat
     7981 aattaaaaca tcaatgaaag aacaaaatat gtcactaagt gaattagctc gtcgtgtagg
     8041 tgtagcaaaa tcagcagtat caagatattt aaatttaact agagagttcc cattgaatcg
     8101 tgctgaagat tttgcgaaag tacttggaat aaaaacagaa tatttattag gatttgctga
     8161 acgcgaagaa tctacaaaac aagatactat cgccgcgcac ttagatggag attttacaga
     8221 ggaagaatta attgaaatta gaaagtatgc ggagttagtt agaaaagcac atcgaaatca
     8281 gtaaggggtt attgcatgta tttatacgaa aagatggtta ttgaaaacaa agagatacca
     8341 attgatgatg ggaaatcttt aggtaatttt gagggtctct atgacaatgg ggttatttta
     8401 attaataaaa atttgtcaga aacacgtaaa gcagaagtgt tatatgagga acttgcccac
     8461 cacaagttga cgtatggcaa cattttagac caatcaaaat ggattaatag aaaatttgaa
     8521 aattatgcac gtagacacgg ctttatctca gctgttccat tacgtgaaat tgtagaagct
     8581 tataattatg gcgtacgtaa cttgtatgag ttgtccgagt acctacaact gagtgaaaaa
     8641 tacatattag aagcgataga acaatataaa aagatatacg gtattggtac ccactacggc
     8701 gagtattcaa ttacatttga gccgttgaga gtttttaaat ataaagaaat ataacaaagg
     8761 agaaatgaaa atgaataaaa tatttagagt actcactgtt agcttgtttt tcttcacatt
     8821 tttaataaaa aacaatctag catatgctga tgtaggggta atcaacctta gaaactttta
     8881 tactaattat caaccagaaa cgcttcaagg agttagttct ggaaattttt ctacttctca
     8941 tcaattagag tatattgatg gaaaatacac tttatattca cagtttcata atgaatatga
     9001 agcgaagaga ttaaaagatc ataaagtaga tatctttgga ataagttact caggtctttg
     9061 taatacaaaa tatatgtatg gcggaattac gttggcgaat caaaatttag ataaacctag
     9121 aaatatacct attaatctct gggtcaatgg taagcaaaac actatatcta cagacgaggt
     9181 ttctactcaa aaaaaagagg taactgctca agagattgat attaagttac gaaaatacct
     9241 acaaaacgaa tacaatatat atggttttaa taaaacaaaa aaaggtcagg aatatggata
     9301 tcagtcaaaa tttaattctg gatttaacaa aggaaaaatt actttccatt taaataatga
     9361 aacttctttt acatacgatt tgttttacac cggaactggt caagcagaga gttttttaaa
     9421 aatttacaat gataataaaa ctatagatgc agagaatttt catttggatg tagagatttc
     9481 atatgagaag actgaataaa tagaatttaa ggagaaaatg caatgaaaaa attaatagtt
     9541 atcatattaa taaatataat aactttaagt gtctctaata gtgccagcgc tcaaggtgat
     9601 ataggaattg ataatctcag gaatttttat acaaaaaaag actttgtaga tttaaaagat
     9661 gtaaaagaca atgatactcc tatagctaat caactacaat tttcaaatgt atcttatgat
     9721 ttaatttcag aatcaaaaga ttttaataaa tttagtaatt tcaagggaaa aaaacttgat
     9781 gtttttggta ttagttataa tggccagtgt aacaataaat acatatatgg tggagtcaca
     9841 gctactaacg aatatctaga taaacctaga aatatcccta taaatatatg gatcaatgga
     9901 aatcacaaaa ctatttctac caataaagtt tcgacaaata aaaaatttgt taccgctcaa
     9961 gagattgatg tcaaattaag aaagtacctt caagaagaat acaacattta tggacataac
    10021 ggcactaaaa aaggagaaga atatggtcat aaatcaaaat tttattctgg gtttaatatt
    10081 ggtaaagtaa cgttccattt aaataataat gacacttttt catatgattt attctacaca
    10141 ggagatgatg ggttaccaaa aagtttttta aaaatttacg aagacaataa aactgtagag
    10201 tctgagaaat tccatttgga tgtcgatatt tcttataaag aaacgatata aatctattaa
    10261 ttatgtcata atcattattt tccgggtagc atgcctaccc ttattatttt ttgccaattt
    10321 tgaggaggga gaagcaaaat gtggttagaa aaatttaaaa ataagaataa agaaactaaa
    10381 tatagatatt acgagaagta caaagaccct ctcactgcca aatggagacg tgttagcgtg
    10441 gtacttaata agaatggtaa gcaatcacaa aaagaagctc aaagactctt aaatgagcgt
    10501 atagaagaaa aactgaatga taagaaacct actacactta agtcactaac tttccatgtc
    10561 gcatgtgatg agtggtttca gaattatatc aaaacgtctg ggtcaaaaag aacaactatt
    10621 aaaactaaat tgagtaagct aaacactttg aagaaatttg tagatgaaga tattttaatc
    10681 aataaaataa cactttcata tgcgcaacaa gttttcgatg aaatggatag taaaggttat
    10741 gtatatcaag taaacaaaga tgcgttaagc atattcaaaa atgtatttga atacactaga
    10801 cgtatctata aactgcaaga tttagaattt ttaaaagata taacattaaa taaaagaatt
    10861 aagtcttatg atgaagtgaa agctaaacgt aataagtacc tcgaattaaa tgaaatagaa
    10921 tctatcatca aagatattaa tacgaaggct cagaagatgc actcaggtat ccataagcgg
    10981 ttttatttat tcgttgcact catgacagaa ttccaagcct taaatggtat gcgtattggt
    11041 gaaatgttag ccattcaaaa tgaagatata gactttgata ataagagttt aaatattaac
    11101 ggaacaatcc attggtttca cgatgaatct ggtggattcg gtgtaaaaga taccactaaa
    11161 acagagtcta gttatagaac aattggattg agtagcagaa gttgcgagat attaaagaaa
    11221 gcaatactgg agaacaaaaa ggattcaaaa tggaatgatg gatatctaaa tagaaatttt
    11281 gtatttacta atcataaagg caacccaatg cagactgaaa gattcaataa aatccttagg
    11341 gaagcagcta aagatgttgg tattgataaa gaagtttctt cacatatatt aagacatagc
    11401 cacatatcat tactctctca acaaggcgtg tcacttaaag ctattatgga tcgtgtaggc
    11461 cattccgacc accgtacaac actttccatt tattctcacg ttactgaaca gatggataag
    11521 gatatgatga acaaacttga acaggtaaaa cttggataat gcgctcaaat ctgagcgtat
    11581 ttttttgttt aaaattcgat aatttgcata tactgcccct tttgtgccct tttttatttt
    11641 caataccaca aaaataaccc cttaatcgtt gttattaaag ggtattttta tttaattggt
    11701 attatttagc aggaataact gcaccattgt atttttcatt aatgaagtct tgaatatctt
    11761 tagattgtaa tacttcaatt aatgctttga ttttcttatc atcttgatgt ccttctttaa
    11821 cagcaattaa gtttgcataa ggattatctt tcgcactttc tacagcaata gaatcttttt
    11881 taggatttag tttttgttcg attgcaaagt tcgaattaat gataacagca tcagcgtctt
    11941 cattttgata aattttaggt aagaattctg ctgattgttt attattaaac ttaatatctt
    12001 ttttattctc tgtaatatca ctaaacttag catcttcaat ttttacgcct tttttgattt
    12061 taattaaacc tgcatcaacg aagaatttta agaaacgtcc ttgttcagct ggattattag
    12121 acacatagac tgttgcacct tttggtaatt cttttaaact tttatacttt ttagagtata
    12181 cagccatagg ttctaagtga acatcaccgg cacttacgat tttgtaacct ttatcctttt
    12241 tctctgtgtt taaatatggt gtatgttgga aatagtttgc gtcaatttca cctttgtcta
    12301 gtaatttatt aggtgtagtg taatcgttaa ttgttttaat atctagttca taaccttttt
    12361 tctctaataa tggttttgct ttttctaaaa tttcagcatg tggtgctggt gaagcaccta
    12421 ctgttatttt cttgtcgtca ctgccacttt tgtttccatt accgcaagct gctaatacaa
    12481 ctgcaaatgt taatactaaa ataagaccaa ataatttttt cataaaatga aacccccaat
    12541 ttatcgttta tcaagtttat ttgtaagcca atccccaatg aattggatta taaatacaat
    12601 aattaaaata aaaactgttg atactaaaat gacatcattt tgatttcgag tgaaacctgt
    12661 taagtatgct aaatttccta aaccaccggc accaattaca cctgcaactg ctgttgaacc
    12721 aactaaagcg attgctgtaa ctgtaatgcc agacactagc gctggcatag cttcaggtaa
    12781 aaggacttta cgaattactg tccaagtatt agcgcccatt gaccaagccg cttcgatgac
    12841 acctttatca atttctttaa aagcaatttc tacgagcctt gcataaaacg gtgctgcgcc
    12901 aatgatcaag gctggtaacg cacctgtcgg accacttatc gttccaagta tcaaacttgt
    12961 aaatggaatt aataataaaa ttaaaataat aaatggtatc gctctaaata agttaacaat
    13021 gaaagaaacg atagaataaa ataaccttgc accgatagac ttacctttag cagacaagaa
    13081 taataacaca cctaaaataa gaccaagtat aaatgcaaat atagttgaga cgactgtcat
    13141 gtatagtgtt tcgactattg cagtccaaac ttctggccac tgaatattag gcattgtaat
    13201 catttcattt ataatttcac taaatgattt acccatgtct taacacctcc attttaactt
    13261 gtcgctcaat taactctttt tcgaattttc cgaaatctac acttgaaata tatggaatat
    13321 gcagaactaa aaagccgact gttccatttt ttgtattttt aatatttgct tctaaaatat
    13381 taattttaat atcataggca gttgatagac tcgatacaat aggctcggtt gttgttgaac
    13441 cagcgaaaac taatctaacg atatatgcat ctttttctaa tggctctaat tctgttaaag
    13501 atgtttcgaa atcatcattt aaatcgtctt tcacaaatcg ttttgtcaca gtgtgttgcg
    13561 gattttcaaa aacctgtgtc accggtcctt gttctatcac tttaccactt tccataactg
    13621 caacttcatc acaaatacga cgaatgacat gcatttcatg cgtaattagt acaattgtta
    13681 aattttgttg ttctctaatt tttagtagta gatctaaaat ttcatctgtt gtttgcggat
    13741 caagtgcact tgttgcctca tcacaaagca agaccgttgg atcattagct aacgctcgtg
    13801 caatcccaac acgttgcttt tgtccacctg ataactctga tggataagcc ttttctctac
    13861 cttttaaacc gacgagttcg acaagttcta atgctttttg cttagctctc cttctaggga
    13921 cacctgcaat ttcaagcgga aacataatat tttttaacac agtccttgac cataacaaat
    13981 taaaatgttg gaagatcata cttacttttt gtctttttgc tcttaatcca tttttggaca
    14041 attgacctat atggtctcca tctataataa cttcacctga tgtaggcgct tctaaatgat
    14101 taaacattcg aatcaaagta ctttttcctg ctccagaaaa accaatgacg ccataaatcg
    14161 atcctgctcg aatcgataaa ttaacgtgat ctacagcaag gacttcttta tttttagtcc
    14221 gatattcttt aacaacttct tttaattcaa tcacgttgat tcctccctgt gttgcttaat
    14281 aaaataaaaa atgctttctc aatatcgata gaaaaattga gaaagcaata gtagtattgt
    14341 ttctctcatc ttcaaaagtt aaaactttat gtgaattggc accatttcta tataagacgg
    14401 ttgccgggct tcgtagggca catccctcca ccactctcga taagagttta cgcatcattt
    14461 aatttgtatt aatcctaaca ccttagtaaa atttcgtcaa taactatttt aaattttcta
    14521 acaaatcagt caccgattta aatgcataaa ttcgttttac ttctttatct ttattcatca
    14581 acaataaaat cggcgtagac atgatttgca tatctttaca a
//
LOCUS       DAKTGS010000069        14567 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.069, whole genome shotgun sequence.
ACCESSION   DAKTGS010000069 DAKTGS010000000
VERSION     DAKTGS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.069"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            40..648
                     /locus_tag="O0286_001663"
     CDS             40..648
                     /locus_tag="O0286_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCY0170042.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            985..2193
                     /gene="gltS"
                     /locus_tag="O0286_001664"
     CDS             985..2193
                     /gene="gltS"
                     /locus_tag="O0286_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCY0170043.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(2241..3290)
                     /locus_tag="O0286_001665"
     CDS             complement(2241..3290)
                     /locus_tag="O0286_001665"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCY0170044.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(3321..4268)
                     /gene="corA"
                     /locus_tag="O0286_001666"
     CDS             complement(3321..4268)
                     /gene="corA"
                     /locus_tag="O0286_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCY0170045.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            4365..4856
                     /locus_tag="O0286_001667"
     CDS             4365..4856
                     /locus_tag="O0286_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCY0170046.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            4853..5272
                     /locus_tag="O0286_001668"
     CDS             4853..5272
                     /locus_tag="O0286_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170047.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(5380..6300)
                     /locus_tag="O0286_001669"
     CDS             complement(5380..6300)
                     /locus_tag="O0286_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0170048.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            6508..7146
                     /locus_tag="O0286_001670"
     CDS             6508..7146
                     /locus_tag="O0286_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170049.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(7392..9323)
                     /locus_tag="O0286_001671"
     CDS             complement(7392..9323)
                     /locus_tag="O0286_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCY0170050.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(9336..9983)
                     /locus_tag="O0286_001672"
     CDS             complement(9336..9983)
                     /locus_tag="O0286_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCY0170051.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            10104..10655
                     /locus_tag="O0286_001673"
     CDS             10104..10655
                     /locus_tag="O0286_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0170052.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            complement(10885..12093)
                     /locus_tag="O0286_001674"
     CDS             complement(10885..12093)
                     /locus_tag="O0286_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0170053.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(12203..12331)
                     /locus_tag="O0286_001675"
     CDS             complement(12203..12331)
                     /locus_tag="O0286_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170054.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            complement(12362..13744)
                     /locus_tag="O0286_001676"
     CDS             complement(12362..13744)
                     /locus_tag="O0286_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170055.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(13984..14439)
                     /locus_tag="O0286_001677"
     CDS             complement(13984..14439)
                     /locus_tag="O0286_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0170056.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
BASE COUNT         4768 a         2548 c         2086 g         5165 t
ORIGIN      
        1 ttttatttaa attaatcata taattgcgag gagaatatta tggatttcgt taataatgat
       61 acaagacaaa ttgctaaaaa cttattaggt gtcaaagtga tttatcagga taccactcaa
      121 acgtatacag gctacatcgt ggaaacggaa gcttacttag gtttgaatga tcgtgcggct
      181 catggctatg gcggtaaaat aacacctaaa gtcacgtcat tatataaacg tggtggtaca
      241 atttatgcac atgtcatgca tacgcattta ctcattaatt ttgtaacaaa atctgaaggt
      301 atacctgaag gcgtacttat ccgcgcaatt gaaccagaag aaggtttatc cgctatgttc
      361 cgtaacagag gtaagaaagg ctacgaggta acgaatggcc caggaaaatg gactaaggca
      421 tttaacattc cacgggctat cgatggcgct acgttaaatg actgtagatt gtctattgat
      481 actaagaatc gtaaatatcc taaagatatt attgctagtc cacgaatcgg tattccaaat
      541 aaaggtgatt ggacacataa atctttacgt tacacagtga aaggtaatcc atttgtgtct
      601 cgcatgcgta aatcagattg tatgtttccc gaagatactt ggaaataaat gccatctttc
      661 attgattact atcatgaaaa tgaaatctat ctccttataa gtcaatcaat cgtgccgtca
      721 acatgcggat gggttgattg tttttctttg tatccatcat attttttgat tcatctcctc
      781 ttattgaact tgttcttaat tataaaatat aacaatagaa ttatttataa ttattaaatt
      841 tagatgcatt aatattattg atattatttt caaaaactag aaatattgat ttgttgcatg
      901 tataatgtta aaagcgccct tttataacgc ttacatataa aagcttattt agggagaggg
      961 atattcaaca agggggattt gaaaatgata gaacttaatg caattacaac attatgttta
     1021 gcttgtatcc tttatttact tggtaaggct atcgttaatc acgttaattt tttaaaacgt
     1081 atttgtatac cagcaccagt gattggcggc ttaatctttg ctattttagt tgcggctttg
     1141 gattcatttg gcatggttaa gattaaatta gatgcttcat tcattcaaga tttcttcatg
     1201 ttagcattct ttacgacaat cggtcttggt gcatcattga aattatttaa attaggtggc
     1261 aaagtcttgc tattatactt tatgttttgt gctatcattt cagtcattca aaacatagtt
     1321 ggtgtatcac tagcaaaagt attaaatatt aaacctttgt taggattaac agcaggttcc
     1381 atgtctatgg aaggcggtca tggtaatgct gctgcttatg gtaagacaat tcaagattta
     1441 ggtattgatt cggcactgac agcggctctt gcagctgcaa ctttaggtct tgtatttgga
     1501 gggcttatcg gtggtccagt tgtaaaattt ttaatcaaac gttataactt aaagcctcaa
     1561 catagtgacg atacatttaa agattatagc caagtagcat ataacgaaca tttacatagt
     1621 aaatttaatg ccactgaagt attcttcatt caatttacaa tcgttgtatt ctgtatggca
     1681 gttggaagtt atttcagtca tttgtttaca gctcaaacag ggattaatgt tccaatttac
     1741 gttggctcat tatttgtagc tgttattgtc cgaaatatct ctgaaagttt taattttaat
     1801 attgtagatt taaaaattac taatcaaatt ggcgatgtcg cattaggtat tttcttatct
     1861 cttgcgctaa tgagcattca attaatcgaa atttataaac ttgctatacc tcttattatt
     1921 atcgttttag ttcaagttgt cgttatgatt ttatttgctg ttttaatttt atttagaggt
     1981 ttaggaaaag attatgatgc tgcagtaatg gtaggtggtt ttatcggtca tgggcttggt
     2041 gcaacgccaa atgccatggc aaatttagat gttattacta aaaaatatgg aaactcacct
     2101 aaagcatatt tagttgtacc tattgttggt gcattcttaa tcgatttaat tggtgttata
     2161 gtcattatgg gattcataca atggtttagt taaacaccaa actcataaat aaaagaggag
     2221 gccttcgcct cctcttttat ttatcctcga tgtatattca agttacgttg ttctatccat
     2281 gacaatattt ccggactaaa tacgatttgt ttttgtgtta agtcgtcaat atttttagca
     2341 tctaacatcg tcattattga tttcatgtgt tcaataaatg attctacata agctactgta
     2401 tgtgcaatgc cattattttc aacttgattt aaaaacggac gtgacatacc agttgccttt
     2461 gcaccaagtg ctaaactttt aattgcatcg agtggtgtac gtaaaccacc actcgcgaaa
     2521 actgaaattt cgctttgata agccgttgtt tcaagtaatg actcaactgt agactgtccc
     2581 catgatgata agtaatccat atctttattt gcacgacgtt cattttcaat atctacaaag
     2641 ttagtaccac ctttgccact aacatcgaca tacttgacgc ctatttgttg taagtcatgc
     2701 attaattctt tgctcatacc aaatccaact tcttttataa tgactggaac agacactcgt
     2761 gatacaatcg acgctatatt atctaaccaa gtcacaaatt cacgattccc ttcaggcata
     2821 actaattctt gaggagaatt aacatggatt tgtaacgctt gtgcctcaag taattcaact
     2881 gcttccaaag ccttttctac tggtacgtcc gcaccaacat tgctaaaaat catgccttca
     2941 ggattcattt ttcgcgcaat cgtaaacgtc tcagccatgc gtggatttct caatgccgca
     3001 tgtgttgatc caactgccat cgctaagcca gtttctcttg caactacagc tagcttttca
     3061 ttgatgtttt tcgtccactc gctaccaccc gtcattgcat taatataaac cggatatgcc
     3121 atcgttaagt caggcgtctg tgatgtcaaa tcgatatcat ttacattaat tgatgggata
     3181 gaatgatgca caaaacgcat cttatcaaaa tctgaatgca ttgcgtcaga ttgggccatt
     3241 gctatttcaa catgttcatt ttttctctgt tctctttgaa aatcactcat gattaaacct
     3301 accttttcgt catttctaat ttaaaaccat tttttacgtc tgaaaaagat gattaatacg
     3361 acagctataa caaacattac agctaagcaa atataatatc cataatgtaa atgtaatgcc
     3421 ggcatattta caaagttcat accataaatc ccagctatga atgttaacgg tgaaaatata
     3481 actgatacta atgtcagtac ttgcataata ctattcattc taaatgacgt gtatgactca
     3541 aaattttctc gtatttcgtt tgtcatttct tgagcagtac gaatgatatt acgttgctta
     3601 atcaagtggt catcgatatg ttgaatgtat agcgaatgtt tattatctat aatcaaatca
     3661 ccattttgtt tcattgtatc aattagctct tgcataggaa acagtacacg ttttacttta
     3721 atcaaatccg aacgtaactt aaagacacta tccatgacca ttttattaaa gcgatcatct
     3781 acatggcggt cttcaaaatg ataaacacta tcttcaagtg catatacaaa gttgaaatat
     3841 ttatcaacca tcatatctaa aattaatatg acgacatctg cacaatctaa ttctgcatct
     3901 aatgtattca tatacttata gactacttta tttaatgatt ccaacgtttg atgatgatat
     3961 gttactaata cattgtcttg tataaaaata tttagtgcta ttggtgaata gtttgacccc
     4021 ataatactat ggaatactaa gtattgataa tctttataag atttatattt agctcgtggc
     4081 ataccgttaa ttgcatcatc cacttctaaa tcattaaaat taaaatgtgc tttaaaccat
     4141 tcattttctt gttcattcgg ttcatcaaaa tcataccaaa caatagtcgc atcttttggt
     4201 atctctttga tatcatcaac tactttaaac ggttcatatg tagtttgata ccgtatcttt
     4261 aaagccatcg atactccccc taaataacga attctctatt attttatcat gaattaaata
     4321 acgtgtatgt cttaatttat tttagtatga tagtcactaa ggagatggtt attatcaaac
     4381 aactttttac acatactcaa accgtaacat ctgaattcat tgaccataac aatcatatgc
     4441 atgatgcaaa ttataatatc atttttagtg acgtcgtgaa tcgttttaat tacagccacg
     4501 gtctttcttt aaaagaacgc gaaaatttag catatacgct atttacacta gaagaacata
     4561 cgacatacct ctcagaattg tctcttggcg atgtatttac tgttacttta tatatttatg
     4621 attacgatta taagcggttg catttatttt taacattaac taaagaagat ggtacactag
     4681 catcaacaaa tgaagtaatg atgatgggaa ttaatcagca cacacgtcgt tctgatgctt
     4741 ttcctgaatc attttcaaca caaatagcac actattataa aaatcaatca actatcactt
     4801 ggcctgaaca attaggacat aaaatagcaa ttccacacaa aggagcatta aaatgacaga
     4861 tgcattacaa caaaagattc atatcgaatt actagattta ttagatgatg ttaagtttga
     4921 attaacagaa ttaaatgcac aaaaagggtt atacattaac ggaccagcaa atcagctact
     4981 taagcgtggc gtgcatatgg cttatgttca aggacaaaag caagccatcg ataatattat
     5041 gactattgtg gaacaacagc ttgaagatca acatttccta gaacattatg ataaatttca
     5101 aaatgaggtt gctcatcgca attatgataa aacagctaac tttgcagaac ttagtgatat
     5161 tccacgtcaa ttcgataatt ttttagatca attttatcaa attaaagggc aatactttat
     5221 catcacacat atcaatacac ttattggtga ttttcactca gaagctcatt aacaattagt
     5281 ctatataacc cttgctatat tttcaaaaac aaaacccaat tacgttttca tgtcaaatat
     5341 catcttgcat gaaatcgtaa ctgggtcatt tatatgttat tagttatttt gtgttacatc
     5401 ctcatctatc gatttggcaa tttgtttaat agctttatgt gattgtctaa ttggataaat
     5461 tggaaaatca tgtaccatct taggataatc ataaaactca atgtattgat gatgttgcaa
     5521 catcatttgt tcaaatagct tcatatcagg atgtgtcatt tcacgtccac caccaaacat
     5581 ataaactggt ggcaatcctt ctattgtgcc attaattggc gatatgcgct tatctgttaa
     5641 tggtaggcca ttcgcccatt ttttcataat ctcattgaca ccaaactgac ttagaaccgc
     5701 atcttgttcg attaaggcgt ccgaaatatc tttattagat agtgttgcat ctaaaattgg
     5761 tgagattaaa tacaatttat tcggtaatgg ctgttgatta tctaaaagag attgtacaaa
     5821 ggataatgcc agtgcaccac ctgaaccatc acccatgact acgacatttt gatgtcctac
     5881 ttcagatact aattgatcat aaacacgttg tatcgcttgg aaagtatcgt caatatgaaa
     5941 ctctggtgtc tttggataga taggcagtac aacctcatat aatgtactta aagtgatttt
     6001 atccaacaat ctccaatgga acggtgatgg ttgtagtgca ttgaatccac cgtgaatata
     6061 taaaattttc ttatcaattt gatgtctgaa attaaagcga aagacttgca tatcatctaa
     6121 tgacaatttt tctaaatttg ctttaacatt taatgttgaa ggctgcttat gtttttttct
     6181 attttcaatt tctcttttat aaaaaaatct ttcaacatct tgatcatttt taaacataat
     6241 cgagcgattg tgaagcaaat atttattgac aacgctattc ataacacggt ttctaatcaa
     6301 tgtcttaacc tacctttata tattttatgt atccaatgat tgtctatccc ctacattctt
     6361 tgccaaaaaa agtatataat gtagaagata ttttcttttt cactttcaaa tttaagacta
     6421 caattgaaca gtgatttttc atcattataa cagacaacta gacatattga taagtaaaga
     6481 aaagaacttt atacggaggt accttgcatg acaaatccaa atcaacgatt agaaccattt
     6541 gatgagacat ttcaacaacc gaatattcat cgtggtaagc gatatggtaa gaaaaaacgt
     6601 tcattggtaa gcatgattat tcaaatcatt gttgttatat taaccgcaat cgctggatac
     6661 agcatgtgga aacaacccat ttttaatatt gtcttcacca aagaatctgt agattttgat
     6721 ggcattaaaa attttaacga gacaattgga aaaatgcatc aattaaatat taatttgggt
     6781 aatatcaatg atttacaaca aggaatcgat cgcttaattt tagtctttaa cggtttcttt
     6841 attttatgta tcgttagcct agtcataaca atactgacaa ttgtatttaa tcgcacagtt
     6901 ctaaaagtag taaatatttt agtgttaaca attatgttag tcattacatt gtatttcagt
     6961 tatattatta aagcactcgc acaagaaatt gcagatgttt tcaaaaaata ttatttaaat
     7021 gtaccaccag atcaagtgat tacagaagct gacgctattc ataacggcat cattttactt
     7081 ggctgtagca tcggtttact tgttataagc ttattcttcc gtaatcgcat gcctcgttta
     7141 aaataatgtt atatagtagg gattgggaca gaaatgttat tttcacaaaa ttcatttcgt
     7201 tgacccactt cctgcttttt ctttattaat gacctactac gtcaatgcca aattttaata
     7261 taattcctct aacattctat aaatcaacaa ataaaaaaag tatcgataac catatttggc
     7321 accgatactt taaaaatcat gtccccacaa cataattttt gattagtcac acttcaatta
     7381 atttatttta tttattcatg attgatacta ttatctgcat ctaacttttg agctgtagct
     7441 tttgcttttt tattactttg taaaaataaa cataaaatta aggcgatgat gctaaatatc
     7501 gttgcaacta taaatgcatc attaatacct tcaaccgttg ctagtttatt tacaaattgt
     7561 agtaacactt tcattgcgcc ttcttgtccg ccatattgtg atgctaattc atgcatatga
     7621 tcttgtacaa caggattcgt tttatctaac tcttccccaa aagctgataa gtgttgtgta
     7681 gtttgtgttg tcattacagt aactaagatt gctgtaccta tagagcctgc taattgacgc
     7741 atcgtattta agaaagcatt accatgagag gcaagtcgtc ccggtaacgc attaatagct
     7801 gcagttacca ttggcatcat tataaatgcc ataccaaatg aacgaagtac atagataccc
     7861 atgattgtca tatatggtgt atccatattt aatttagtta attcccatgt tgcataagtc
     7921 attacagcaa taccaaagat agctaatggt tttaaaccaa tagtatctaa caatttacct
     7981 gcaaatggtc ctagtagacc cataattaga gaaccaggta ataataacaa tccggaatct
     8041 aatgctgaga atccgcgtaa attttgtaaa taaatcggta ataaaatcat accaccatat
     8101 aaacttaaca ttacaaccat attaataatt gttgttaatg taaatgttgg gaatttcaat
     8161 acttctaaat tcaacattgg tgatttcatt cttaattctc taataacgaa tagaataata
     8221 aagataatac caatcgcaaa cattgtttct atctctactg aaccccaacc tttgttgcca
     8281 gcttctgaga aaccatataa caaagcacca aaaccaatcg tactaaaaat gatacctggg
     8341 atatcagctt tagggtttgt tgtatattga tataacttaa accatacaaa accaattaaa
     8401 atagcgataa tcccgataat gaacataccg taaaacatca cattccaatg gtaattttgt
     8461 acaatataac ctgataatgt tggaccaatt gcaggtgcta aaatcattgc gatacccatt
     8521 gtacccatgg cagcaccacg tttttcaggt ggataaattg taataataac aattgaacct
     8581 aatggcatta gtacacctgc accaatggct tgtaatacac gtccaaccat catgattggg
     8641 aaattcattg aaatcgcaca gattaatgaa ccaattgtaa agagtactaa cgcaactaaa
     8701 aataattttc gatatgaata tttattaaat agatacgccg taattggtat taaaataccg
     8761 tttactaaca tgaatcccgt catcaaccat tgccctgttg acgcagaaat attaaattcc
     8821 gtattaattt ttggtaaagc aacatttaat aatgtttggt ttaaaatcgc aataaacata
     8881 ccgaataata atgccgctaa tattttaccg cgtgaaacac cttcaccaaa aataaagttt
     8941 ttatgttctt tttttatttt ttcattcact ttatattctt ctgattcagg atttttagca
     9001 gcaactgctt cctcatcctt attattagtg aatgcttctt gatctttctc agaccccttt
     9061 gtgtaaccat ttagactaac ttggctatga tcatcttgat tgtcaacacg caactcttca
     9121 tgcgtcatac gttgtgtcga ttgatcagtt gttttgtcta aatcactagc tttaaattta
     9181 gattgatttg attgacgtgt cgtaaattgt tgttcctttt gttggcgttt gccttttttt
     9241 cttgatctta ttaaaaataa attgataacc ccaacaataa tgagcgctaa aataatgtag
     9301 ctaataatga aggtcgtagt catttaatga cccccttaat ttttatggat ttttacttca
     9361 gcgttcattc caggaacaac ttgtttagac ggttctgatt ctagagtgat tttaacaggt
     9421 attacttgag aaactttagt gtagttacca tcactatttg atgatggcat taatgaaaag
     9481 cttgcagcag ttgcttttcc aatactatca actttacctt taatagaagc tttttgaccg
     9541 tcaatagtca catcaacatc tttacctact tcaacatctt taatatcttt ttcgtcaata
     9601 tttgctgtta catataaatc atctaaattg tatgcataag cgattgggtt accagcttgc
     9661 accattgaac cttccatacc atctaatttg gcaattgtac ctttttgagg cattttaaga
     9721 tccatatctt tcgtttcgcc atcttgacct tgtacagtaa caattgctac tttgtcacct
     9781 ttatcgagtt tgtcaccttg tttaacatta agtgatttaa tttgtccaga tgcaggactt
     9841 gctattttaa tttgatcgcc atttactttt gcattatcag ttgttacata gcttgttgtt
     9901 ttattccaaa aataaaagcc agcaatccca attgctaaca gtacaacaac cgtaatgaca
     9961 ttaattaata tcatcttttt catgaaacat ttcctcctat ttgcttttaa aattcacttt
    10021 atttattata gagacatgat aactaaaaaa acaccaacaa tttttataag agtgttggtt
    10081 aaaattcaca aaggtgtaac aatatgaaac gacaagccaa aatagaaatt caaaacgcac
    10141 ttgttgattt gatggctgaa tatccttttc aagaaatatc cactaaaatg atttgcgcgt
    10201 attgtaatat taatcgttct actttttatg attattacaa agataaattt gatttattgg
    10261 ataccatcaa ctcaaagcat aaggaaaaat tccaatttct tctaagcgcg ctacatcata
    10321 atttcgaaaa tattaaacag gataagctta aactttataa attcttcatt attattgcta
    10381 agtatatcaa gcacaatgag cagtttttca aagacatttt agttacctat ccaatgaaaa
    10441 cattattcat agactatata aatttagcga gagattacta tcaacaaatt atgaatgact
    10501 atagtactac agtaaccaat aaacagcttt atgttacata tatcataggc ggtcaagctg
    10561 gggtgtttat taattggctt tcgaatggtt gtaatgaatc acctgaagaa gttgctgaca
    10621 ttttattggc taatacgatt aaacttcaaa actaataata cggtttcatt ctaataaaga
    10681 aatgctataa aacaactaga taaagttaaa ccaaatggat agataatagt acatatgtaa
    10741 cggtgctatc aatataaaaa tgccgagatt tccacataat aatctcggca tttttatttt
    10801 tgatgctaaa ttcataggtc tatgaatagc tcaagttttg ttttttaatt tttcaattca
    10861 atataataga agaaactatg tttatcatcg atttgtttta aataccttca tattataaat
    10921 ctgtaaaata attaatataa ctgctgtagc tataataata attatgtaag gtataggatt
    10981 atcttcacct tttaccccca ctaatggcga tgcaacacca ccaaataaaa actgtactaa
    11041 acctaataaa cttgatgaac tccctctacc actactactt tcatccatag ctattgtaaa
    11101 accaagtgtc gcaacacctg tcactggtgc tattaaaatg ataaatccta tcgctaaaat
    11161 ccaaaagttc caatggttaa gcaatacaat cgtcactaaa attacaccga taatctgaat
    11221 catcgtcatg cctctcatca atttttgtga gtctataaaa tcaacaaggt acccagtaag
    11281 ttgacttgat ataatgagcg taataccaat tcctgcaaac atccaactaa attgtattgc
    11341 agtcatgcca taaatttttt gaataataaa cggagacgca gaaatataag taaataatat
    11401 tacaaatgtc ataccttgaa tgagcattgg taatacaaaa cgtggcgtct tcaataatat
    11461 tttgaagttt ttaaacattg tctttaatcc actacttgac tcacgatttg tcactgttaa
    11521 tgattcaggt acttttaata aagaacctat gaccatgacg aagccaaaga tagtcagaat
    11581 gacaaagacc atacgccaga cagaataatt taaaattatg ccccctattg ttggcgcaac
    11641 aactggtgca ataccattaa caagcatcaa taatgccata aacttagtta gttcattacc
    11701 actatacata tcacttgcta tagctcttga aataactgct gctgcgccac ctgtcactcc
    11761 ttgaagaaat cttaatgcaa ccatcagcca aatattatgt acaaaaacaa tacctaaact
    11821 tgctaatgta aaaataatca tagctataat aagcggcttt ctgcgcccag ttgaatctga
    11881 aattggacca gcaaacaaat taccaaacgc taaaccaatc ataaacatcg ataatgttaa
    11941 ttgtgcgtta gacgtcgttg tttgaaaatc atgtctaata tctggtaatc caggcaaaaa
    12001 catatctatc gataatgctc caatggccgt caaagatcct aaaataataa taaatatcgg
    12061 tgaacgcttg ttaaaatgcg ttaaatcatt catatgacta tctcctttat ataatgatta
    12121 gtttataact atcacgaaca gaaactaaat ttaaatatgc tcttttatat attagcatta
    12181 gtcacaagtt tcatgaacat cattacaact tataaaaggt ataaaatgat ataaacctca
    12241 gcacttcata aatttttcca caaaaaacaa tcccttcaaa gtaattcaca ctaattgtaa
    12301 atactttgaa aggatgttgt ttgtattcca tgacacatgc atcttattta agttttatac
    12361 tctatttttc tgatgtcttg attaattgca agcggtcttg cgagccttgt tcaagctcat
    12421 attctttatc tacttgctta ccgttttcat ttattaatct tacggttgcg gtaatcttat
    12481 caccatgttt ttcagcatct ataatttgtg gtgaactaat catcattaaa cttcctttag
    12541 atacacgctt ctttacatca tcataaaaag atgatccttt ttttatatat gatgatacaa
    12601 aatcaaaatc cgactgatta aacgcagcat tattcgctaa agaatatcca gcaaagaatt
    12661 ctatcaattt gttcttcaag ctgttttctt ctttttcttt cttttcaaca tagtcttcga
    12721 tatcttcact gtcaaaattc aaagttatct ctgtattgta ttttaaatcg ctagctttaa
    12781 tcgtcttcgt ctgtgttgta aatgttttat ctttcgcttt acctgaagct gaaatggtaa
    12841 tatctttatt ttgtggatat ggaccatacg ttttggaact tgaaaatgcc atttcatggt
    12901 catttatagt tactttttta gaactatcat ttaattttgt atcgcctttt aaagttacag
    12961 atatatttgc ttcttcaaaa tcttcagtaa catctaccgt ttcagaatta ctttgtctaa
    13021 aatcaaattt taaatggcct gcaaaatcac cgttctcagt tgatttcata gctggaattc
    13081 tatatgtccc cggtataaaa ttacctattg gcgtcacttt atttgcttca gctataacca
    13141 tctttttctt accaccagat ttaaactcat attttgtttt ttctttcggt ttaacaattg
    13201 gttgcttggt aggtgcagta aagctcatat tatcgaaaaa gatataacgt gtgccatttt
    13261 tacttacacg taatatattt tgaccagatc tggtttgaat ataagaagct acgctggtct
    13321 tactcttatt caatttatgt accgtatttt taaggtcgct gacaaattgc ttaagcccaa
    13381 cttcatcttt gatatagtta atgtatactt ttgcttcttc agaatctact ttattatctt
    13441 tagtacttaa taatgttgct actttttgtt tatcattatt ctcaatcgca ttcactaata
    13501 ttttagtttg cgcttcaggt gaattgaagt ttcttagtaa aaagaacaat ataataatta
    13561 agattaaaat gaaaaaacca attgcccaag gcaccatttt tctcatcttg atgttattag
    13621 tttgtatatc ttcatcggtt gactttctat ataatgcttg acgactatca aatttatgtc
    13681 cacattgtgt acatatctgt acatcatctt gtgcttgttg tccgcacttc gggcaagatt
    13741 tcatactttc acctcaattc tgttcctctt caattgttat atgtatatca caaaaattat
    13801 ttcattattt taccattatt attttacaaa tattatccca agaatacaat tcaagaaatg
    13861 attaacttta ctttagaatt gtaccttatt acattattta tgaaagtact ctctcaatta
    13921 taactaaaaa gaaggtgttc aatacataca tatttagtct tatatttgaa caccttcaaa
    13981 aacctattta tgattgctat atgttttaat gcgatgattg atgacttcta atacttgcct
    14041 tacattttca atatcttcag aatttaaatc attagtaata tcttgcgcaa tatctgtcat
    14101 aatcgcatta cgttctttca aatatgctct acctttgtca gttaaggtta tgaatttcaa
    14161 acgttgatca atatttaaat ttggtttatc taacttaact aatttagcat cgattaactt
    14221 tttaattcgt cggcttactg cggccttatt tacaccttgt ctttgcgtga tttcactaat
    14281 tgtcaaaggt tctttattta acatacctaa tacgttagac tgctctaatg aaatttcata
    14341 ttcattttgt aaatctttca acatttttgc agttaatgcg ttaacgttat ttataaactg
    14401 ctctaaaaat tgaatatggt cttgtaaatg ttttaccata attatttctc cctaattact
    14461 atttttataa agtatcctca gcaatcattg tatcgcacat ctaaaaaaca caaaaatatt
    14521 gacccatata taatataaca aattaagatt tacatgttgc tgctttc
//
LOCUS       DAKTGS010000070        14303 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.070, whole genome shotgun sequence.
ACCESSION   DAKTGS010000070 DAKTGS010000000
VERSION     DAKTGS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.070"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O0286_001678"
                     /gene_synonym="sfnaD"
     CDS             complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O0286_001678"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCY0170057.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2200..3393
                     /gene="sfaA"
                     /locus_tag="O0286_001679"
     CDS             2200..3393
                     /gene="sfaA"
                     /locus_tag="O0286_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCY0170058.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3380..5137
                     /gene="sfaB"
                     /locus_tag="O0286_001680"
     CDS             3380..5137
                     /gene="sfaB"
                     /locus_tag="O0286_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCY0170059.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5144..6214
                     /gene="sfaC"
                     /locus_tag="O0286_001681"
     CDS             5144..6214
                     /gene="sfaC"
                     /locus_tag="O0286_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCY0170060.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6315..6440)
                     /locus_tag="O0286_001682"
     CDS             complement(6315..6440)
                     /locus_tag="O0286_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170061.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6606..7589
                     /locus_tag="O0286_001683"
     CDS             6606..7589
                     /locus_tag="O0286_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0170062.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7601..8632
                     /locus_tag="O0286_001684"
     CDS             7601..8632
                     /locus_tag="O0286_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0170063.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8629..9597
                     /locus_tag="O0286_001685"
     CDS             8629..9597
                     /locus_tag="O0286_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0170064.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9833..11203
                     /locus_tag="O0286_001686"
     CDS             9833..11203
                     /locus_tag="O0286_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0170065.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11335..11595)
                     /locus_tag="O0286_001687"
     CDS             complement(11335..11595)
                     /locus_tag="O0286_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170066.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11772..12287
                     /locus_tag="O0286_001688"
     CDS             11772..12287
                     /locus_tag="O0286_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0170067.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12331..13518
                     /locus_tag="O0286_001689"
     CDS             12331..13518
                     /locus_tag="O0286_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCY0170068.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13537..14220
                     /locus_tag="O0286_001690"
     CDS             13537..14220
                     /locus_tag="O0286_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCY0170069.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
BASE COUNT         4743 a         1989 c         2610 g         4961 t
ORIGIN      
        1 cctgtttttt gtgattttta ataatttgaa tatggaaaat gtattattct ctcatttgta
       61 tagattgtat ttaataagtt aatgtaatcc ttgagctcac gattaataaa attctataac
      121 cttaattatt ttctcgatac aaagggttat taactttaat ataagtataa tgatgtgcct
      181 catcttcaag acgcattgtt gtaacacact tataatctat aaatggcgcg aacatggtat
      241 ctttaatttc atttatgcga ttctcattta cttgattaga tttgtgtgtc gaaagtacaa
      301 gttgatcaaa aatgttatct agtacatcac gaacgatata ccacatatgt ctttctaagt
      361 ttgaatcatt tgatgcttta gagattgtaa gaattaattc gcctaaatgg ttttgaacgg
      421 tagaataaaa ggctttgtta aacacagacg tttttgaatc agtaagaatt cttgattttt
      481 catggaaatg agatgtactg tatcccattt catttagttg tgctttatca attcttaaac
      541 cttcaaagtc tctaatatac atcgtgtcca atagaccatc tttccgaaat gttgcaatag
      601 cattttgcaa gtgagcctct aatgcaatgc catatttagt aactagtggg attacgagac
      661 caagcaatgc tttactataa gtttcaaccc acgatttcgc cgatgattca aaatcagaca
      721 atgatgcagc tgattgataa cgtttaatca atgtcacgat aggtgattca ttgttaaatg
      781 ggtaggttgc aactaagctt gaaggaatca atggtgtgac ttcttgtgga atcatttggt
      841 atatattttt tctaaataat gtacctaatt gttcacttcg ttcagtttga tagtctgctt
      901 cgtcttgctc attataaaaa tgaataccag caacttcatc aataattgtt gatgcatagg
      961 acttaaatat gacatctttc tccaaaatat catttaaaat acgtgtcatt agtggaccat
     1021 tgtgcgtcgt ttgttctgat aatgtacgaa tctcacctgt aatatgaacg tttgtcgaca
     1081 atttgatgtg tggcgacata gctgggtatt taggaactaa tgttctgaaa gataaaccag
     1141 cataataatc caacgtatgt tttgcttcaa tgattaattc tttatctact tctgcttgat
     1201 aatcagaatg taatacgtca tctaattgcc atggatgaac aatcataatg tgataatcat
     1261 taaagttaaa ctttggcgta aattcatttt ctaattgttt aattaagtcc ggaaatagtt
     1321 gatgaacagt tgtatcataa tctttagaca gtgacatagt acggcttaat ttactgtgaa
     1381 tcagtactat tttcaactta ataggttgat tgaattctga agaatatagg aatgtttgta
     1441 atgcatttaa acctttacgt aatttagccc caggatgtag cggatgacct tcaataacgg
     1501 cttgctctga acgcaagtaa ctatcttcgc tattttcgat aatattaaat aaaggtgcag
     1561 aatcatgttg cattgacagt gcttgatagc taattgcaaa tgtcatatta gttgcactgt
     1621 ttattaaatc ttgctgaaat tgatcactag cagcattttt taaatctggt gcttcaatta
     1681 aaatacactc aagaatttca tttggatggt gtactcgtgt aatcgtattt gtaatgtcat
     1741 ctttaatgta gaaagggcct tcaacatcaa ttcgatcaaa ggcgtgttct ccagtgatag
     1801 gagcatataa tgtttgctta gcttgtggga actggatttc tagtatatga gtcgttgaga
     1861 tatctaacat aatcaaatca cgactcaata ttttcttact ttgagtgcgc gctttaacta
     1921 agttttcgcg atgcattgat gtgaccaatc tctgagtgac tttatctctt ccttgtaaaa
     1981 tcatctcttt aaaaatatta gcccaatcgc tattatgttg ttgtaaaaat aaatatgttt
     2041 cttgttcttc tttgttaaat tttaatgtct gttctttaaa aattaagttc aagttcataa
     2101 ttcacctcta tgaaatattt tacaaaagca agatagattt gtataatcca tattaatgat
     2161 aatgattctt attatcaaca gaatgcgggt gtaagtttta tgacaaaata tttttttagc
     2221 agttcttttc tactatttct aggtaattgg attggacaaa tagggctaaa ttggtttgta
     2281 cttaccactt atcataacgc agtttatctg gggattgtca atttttgcag acttgtacca
     2341 atattattac taagtgtgtg ggcaggggca attgccgata aatatgataa agggcgattg
     2401 ctgagaatta caatttcatc atcattttta gtaactgcaa ttttatgtgt gctcacgtat
     2461 agtttcactg caattccaat tagcgtcatt attatatatg cgacattaag agggatttta
     2521 agtgcggttg aaacaccttt aagacaagca atcttaccag atttatcaga taaaatatct
     2581 actacacaag ctgtatcatt tcattcattc atcattaata tttgtcgttc aatagggcct
     2641 gccattgctg gtgtcatatt agcagtctat catgcgccaa caacatttct tgcacaggca
     2701 atttgttatt ttatcgcagt tttattatgc ttaccattac attttaaagt aactaaaata
     2761 cctgaagatg catcaagata catgccgtta aaagttatta tagattactt caaattacat
     2821 atggaaggtc gacaaatatt tataacatca ttattgatta tggcgacagg tttttcatat
     2881 acgacacttt taccagtttt gacaaacaaa gtatttccgg ggaaatctga aatatttggt
     2941 atcgctatga cgatgtgtgc cattggtggt attattgcaa cgctagtttt acctaaagta
     3001 cttaaatata ttggtatggt aaatatgtat tatttaagtt catttttatt tggcattgct
     3061 ttgttaggtg tggtatttca caatattgtc atcatgttca tttgtattac attgattggg
     3121 ttatttagtc aatgggcacg tacgacaaat cgcgtttatt ttcaaaataa tgttaaagat
     3181 tatgaacgtg gtaaagtact gagtattatt atgatggata gaggtatgat tccattggga
     3241 agtctattaa tgagtatatg tgcagatgtg tttggcattg ttagaacttt ttcaataatg
     3301 ggaataagta ctatatgcat tacaatggta ttctatttta taaatagaaa gttgaagtta
     3361 aagttggagg aaagtaatca tggtatatct tgaatgggca aaggcagata gaaatattca
     3421 atatcgtgta attaacgcca ttattaaaga acgtatttac cccgagcaaa catttatttc
     3481 gcaaaaagga tctttaattg aaattcagta tcatatgcat gtgttgacta ttgaagttgt
     3541 tagaaaaagt gcattagaac gctatgagtt tacaggtgat attacttatt taaataaagg
     3601 tgaaacgtca ttaattataa ctttagaagg tttattagat gtgttgaatc atgactttga
     3661 tatccctatt tcagagcgac tacgcgaaga gttaatacac agtcgagata gtttagttga
     3721 aacatataag caaatgtctc acagacaaac gttaataagt caaagtttta aattttcaag
     3781 gttaccacaa gatattaact ttttttcatg gttacaacat gtaaaagata gtgataagac
     3841 agatgattta acttattctg agagtttggt accagagggg catccaacac accctttaac
     3901 caaaacgaaa ttgcccttaa ctatggaaga agtacgagca tatgcacctg agtttgaaaa
     3961 agaaatccct ttgcaaatta tgatgattga aaaagaccat gttgtgtgca cagctatgga
     4021 tggtaatgat caatttatta ttgatgaaat aattcccgaa tactacaatc agattcgtgt
     4081 gtttttaaag agtttaggtt tgaaaagtga agactataga gcgattttag tacatccttg
     4141 gcaatatgat catacgatag ggaaatattt tgaagcatgg attgctaaaa aaatattaat
     4201 tccaacgccg tttacaatac tttcaaaagc aactttatca tttaggacga tgtctttaat
     4261 tgataaacca taccatgtta agttgcccgt cgatgcacaa gcaacaagtg ccgttagaac
     4321 agtctcaact gtgactactg tagatggacc aaagttaagt tatgctttac aaaacatgtt
     4381 gaatcaatat ccaggattta aagttgctat ggaaccgttc ggtgaatatg caaatgttga
     4441 taaagatagg gcacgtcagc ttgcttgtat tataagacaa aagcctgaaa ttgatggaaa
     4501 aggtgcaaca gtagttagtg caagtctagt taataaaaat ccaatagatc aaaaagttat
     4561 cgtggatagt tacttagagt ggttaaatca aggaattact aaagaaagta ttacgacatt
     4621 tattgaacga tacgctcaag cattaatccc gcctttaatt gcttttattc aaaattatgg
     4681 aattgcttta gaagcacaca tgcaaaatac agtagtgaac ttggggccac attttgacat
     4741 tcaattttta gtgagagatt taggtggttc tagaattgat ttagaaacat tacaacatcg
     4801 tgtatcagat attaaaatta caaatgatag tttaatagct gattctatag atgcagtgat
     4861 tgcaaaattc caacatgctg ttattcaaaa tcaaatggca gaattaatcc atcattttaa
     4921 tcagtatgat tgtgttgaag aaaccgaatt atttaacata gtacagcaag tagtagcgca
     4981 tgccattaac ccaacactac cacatgcaaa tgagttaaaa gatattttgt ttggaccaac
     5041 aattactgtc aaagcgttgt taaatatgag aatggaaaat aaagtaaagc aatatttaaa
     5101 tattgagtta gataatccga taaaaaaaga ggtgtagtac tacatggcac acgttaacat
     5161 aaatatatcg aagattaaat ataacgccaa agtacttcaa acagtttttc aaagtaaaaa
     5221 tatgcaattc acaccagtaa ttaagtgcat agctggtgac cgtacaattg tagaaagctt
     5281 aaaagcgtta ggtatcaatc atgttgcaga atccagattg gataacataa ttagtattgc
     5341 agatcaggat ttaacatata cgttattgcg tacgcctgcc aaaaaagaga tttcagatat
     5401 gatagaaaaa gttgatatga gtatccaaac agaattatct accatacatc aaataaatga
     5461 agtagcagaa gttttaggaa aaaaacataa gattttatta atggtagatt ggaaagatgg
     5521 ccgtgaaggt gttctgactt atgatgtttt agattatatt aaagagatta ttcacttgaa
     5581 aaatatccat tttgtaggtt tagcattcaa ttttatgtgt tttaaatctg atgcgccaag
     5641 tgatgatgat atctttatga ttaatcgatt cgtctcagct gttgaaagag aaataggata
     5701 tcgattgaaa attatttcag gtggtaattc gagcatgtta ccacaacttt tatataatga
     5761 tttgggtaaa ataaatgaat tgcgaatagg tgaaacgttg tttagaggtg ttgatacaac
     5821 gacaaatcaa gcgatcgcta tgttatatca agatgcgatt acattggagg ctgagatttt
     5881 agaaattaaa cctcgagtta atacacaaac gcatgaatca ttcttacaag ctattgtaga
     5941 tattggttat ttagatacaa aagtcgataa tatttcacct atggatcagc atatcaatat
     6001 tttaggcgct tcgagtgatc atttaatgct tgatttaaat ggtcaaggac actatcaagt
     6061 tggagatcat atatcttttt cattaaatta tgaagcactt tctcattcga tgtatatgaa
     6121 aaatttacat aaggtatata ttgatgattc aaagattgat acgctacttc aaaattttga
     6181 tgtgaaatct cctgctatgg taaaccacta ttaatatatt tatcaataag tctaagttga
     6241 caagttatac tacatatcgt ttgcacggtt gtatcataat tgttcaactt agattttttg
     6301 tatttgttga tttatcaaat taagtgcaac agttcgtcac ataaaattgc aacagataat
     6361 atcagctgaa ttacagggat aacggtcatg ctaaatggtg tcaattgtat taatgcaaag
     6421 atgatatagc aatgatacat tatcagtatt ttgtctaagg aaatgtgcta attgtagtca
     6481 taattattag aggaaatata tagtcataca ttttagaaat acaaaaaaga ttgaacgcta
     6541 cttgacaatg ataattgtta tcaataaaat aataaatgaa gttatacata ttaaggagtg
     6601 gaacgatgag aggtctaaaa acttttagta tattgggatt aatagttgcc ttacttttag
     6661 ttgcagcttg tggtaatacg gataattcaa gtaaaaaaga atcatcaact aaagatacta
     6721 tttcggtaaa agatgaaaat ggtacagtaa aagtacctaa agatgcaaaa cgtatcgttg
     6781 tattagagta ctcatttgca gatgcattag cagcattaga cgttaaacca gttggtattg
     6841 ctgatgatgg taagaaaaaa cgtatcatta aaccagttag agaaaaaatt ggggattata
     6901 cttctgtagg tacacgtaaa cagccaaact tagaagaaat tagtaaatta aaaccggatt
     6961 taattatcgc tgatagcagt agacataaag gtattaataa agaattaaac aaaattgcac
     7021 caacattatc attaaagagt tttgatggag actacaaaca aaatattaat tcgttcaaaa
     7081 caattgctaa agctttaaat aaagaaaaag aaggcgaaaa acgtcttgct gagcatgata
     7141 aattaatcaa taagtataaa gatgaaatta aatttgatag aaatcaaaaa gtgcttccag
     7201 cagtagttgc taaagctggt ttattagcac atccaaacta ttcatatgtt ggacaatttt
     7261 taaacgaact aggatttaaa aatgcattaa gtgacgatgt aacaaaaggt ttaagtaaat
     7321 atttgaaagg accttactta caattagaca ctgaacattt agctgattta aatccagagc
     7381 gtatgatcat tatgacagat catgctaaaa aagattctgc tgaattcaag aagttacaag
     7441 aagatgcaac atggaaaaag ttgaatgcag ttaaaaataa tcgcgtggat attgttgacc
     7501 gtgatgtttg ggcaagatct cgtggcttaa tttcttctga agaaatggct aaagaacttg
     7561 ttgaattatc aaaaaaagaa caaaagtaag gtggaagtaa atggctataa aagaaataag
     7621 tagccaatct gccatagatc ataaaagaaa aagacgcaca acactcacgt atatagtgag
     7681 tttgtgcttt ctttttattt gtatatattt aaatatggcg attggttctt cgaaaattaa
     7741 ttttagcgat atcattcact atgttactgg tcatacagat acgaaagcaa cgtttttatt
     7801 gcataatgta cgtatgccaa ggatgattgc agggttattt attggcggtg cattagcggt
     7861 atctggtttg ttaatgcaag caatgacacg caatccgcta gcatcaccta aaatttttgg
     7921 tgtcagttct ggtgcatcgt ttttcattgt atttgtgacg attatcattc cagcattaga
     7981 atattatgca ttatatttag gtgtgatagg tgcatttata ggtggtttaa ctgtttatac
     8041 actttcaggt gctacgaaag gtatgacacc cattaaatta gcattggctg gtatggccat
     8101 ccatttgttt tttagtagta tgaccgaagg tattattatt ttgaatgaga attcaaacga
     8161 acaagtgatg ttttggcttg tcggttcatt atccagtatg aagtgggatg aaattttaac
     8221 cattctacca tggattattg gtgctttaat tgtaacgatt tttattggac gtcaactaac
     8281 gataatggaa ctaggcgatg acattgctaa gggattgggt caaaatataa ataaagttag
     8341 aattatcatt ggactattag tcattattct tacaggtatg tcggtgtcag tggcaggacc
     8401 aattggattt gttggcttaa tcgtaccgca tatagtcaaa cgctatgtga gtaagaatta
     8461 tttagtcatg attccattaa catttattat aggtgcagat ttgttactac tatcagatgt
     8521 attaagtcgt ttaattacat atccttatga atcaccagta gggattgtta catcattcgt
     8581 aggtgcactt tacttcttat ttataacgat taaaggggtg aaaagaatat gatgacaacg
     8641 aaaattatac gtcgttatag tttaatcaca atccttttaa ttgtgagtat attcataagt
     8701 ttatgtgtag ggtctgtcat gattcatccg attgatgcta ttaagggaat ctttacacaa
     8761 gatgatttta ttttaaatga gtatcgtata cctagaacat tacttggtat tattatcggt
     8821 agtagcttgg ctatttcagg tgcaattata caagctgtaa taagaaaccc gttagcctca
     8881 cctgatgtta taggtatttc taaaggtgcc agtttagctg ctgtgattat tattatgacc
     8941 tttccaacag caccattgtt tgtattacct ataggttcat tcttaggtgc ctttgcagta
     9001 agtttatttt tatcattttt aatttcaaaa tatgatgtta aaggttcaaa gcttgcctta
     9061 attggtttag caataggcgc aatatgtacg gcgattgtgc agttcttatt aatcagaaat
     9121 ccactggaag caaacaaagc gcttgtttgg ttgacaggta gtctatatgg acatgatatg
     9181 tcgaatattt atactatatt accttggttt ttaataacga taccaatcat cattttattg
     9241 agttatcaat tagatgtatt gaatctaggt gatgctgttg caaccgcact cggtttaaaa
     9301 gttaaaacta ttaaaatgct attgttaatt ttagcagtaa tattagcagg atccgcaatt
     9361 tcagttgtcg gtggtattag ctttttagga ttaattgcac ctcatattgc gagacaatta
     9421 ataggaaata aaacattaca cgttattatc atgtcggggt taattggtgc tatattgtta
     9481 acatttggag atggtttagc ccgtggcatt catccgccac tggatattcc tgttggggtt
     9541 atcattgcaa ttatcggtgc accatacttc ttaatattat tacgaagaat gaaataacta
     9601 acatatgatt agagtttaaa aaaggttaag tacgaaatca gtaattgtac ttaacctttt
     9661 ttgaattcat cttgaaaaat tacaagaaat agcgtatatt taataactgt aattattcat
     9721 tattttgcat acataaacat tttgaaagat agaatgataa ggaatatgtt tgttaactga
     9781 atagaattct gtatatactt aaaacgaata catagagaaa tggattgatt ttatggatag
     9841 cttttcaagg aaagagatag ttattgggag acttaagttt attacgatgt ctttaattgg
     9901 tatcttatta tttttagtac caattccagt cgaacaagat ggacagaagc aaacaacgtt
     9961 gcccgtggca tttttagcag gcgtgcttaa agatgtatta ggtggcgtaa tgccattttt
    10021 aattgtgacg ataattactt tatcgggtat cataactctg atttgttcaa ctattttaaa
    10081 agataaattg aaacctgacg ggctaatgaa caatgctttt aacgttagaa ttggttggtt
    10141 aattttacga atattagcag ttgtttttgc atggatgaca tttttacgaa ttggttcaaa
    10201 ggttatttac tcagatgaaa cgggtggatt attgttctca agtttattgc caacattagt
    10261 ggcagtattc ttatttgcag ctctattttt accgctatta atggaatacg gtttgttaga
    10321 gatgttgggt cctattttta gaccggttat gagaccttta tttacattgc caggacgttc
    10381 gacggtagat aatttagcat catttattgg agacggaact gttggtgtct taattacaag
    10441 tagacaatat ggtgaaggat attattctcg tcgtgaggca acagttattt caacgacatt
    10501 tagtgtagtt tctatcacct ttgcaattgt agttgctgaa acagtgcaca tgcaaaatca
    10561 attttttgct ttctatttat cagtgatagt atcctgcctc gttgcagcag tcattatgcc
    10621 aagaatttgg cctttaaata aaattcctga tgagtatgct aaggaggtac ctgaaagtgc
    10681 gcgtactgag gcattgccag aaggaaagac ggcattgaga catggctttg atactgcaac
    10741 agaagtaggt attaaagcac cgggcgttat tgactttttt aagtcaggat taaaaacagt
    10801 cattgatatg tggtttgtta ttttgcctgt agtaatgagt attggtacta ttgctaccat
    10861 tattgcgaac tacacgccct tttttgtcat attgggtaaa ccttttgttc catttttaga
    10921 gttaatgcaa atacctgaag cggcacaggc ttctcaaacg attattatcg gttttgccga
    10981 tatgttttta ccttctatat taattgaagg tgtacaaaat gatataactc gttttgttat
    11041 tggggcactt agtatatcgc agttaatata cttgtctgaa gtaggtggcg tcatacttgg
    11101 ttcgaagatt cctgtaagca tcggcaaatt gtttatgatt ttcttgatta gaacaatcat
    11161 tacactacca attatttctt tgatggcaca tcttttgtta taaatatatg tgaaaaagga
    11221 caactgttaa aattatggga acagttgtcc ttttgtattt gtttatgctg ggtgttgaat
    11281 caattaaaaa aatcattcat gaagccctca ccatgaatga tttttttata caatttattt
    11341 agttaattca ttgtaaactt tttgatcatt ttgagtataa ataatgtatt cgtggtcttc
    11401 agtatcgata ataacgcgat tcgttttacc aaacgttgag ccgattctag atacatcttt
    11461 gctgtctaat ttaggaactg catgaatatc ttggtcttgt gtaatatttt taatttcact
    11521 tgtaggaatt ttgatatcag caactctcca ttgaatacgc acttcattat tatccttttt
    11581 tactgtcatt gccattattc aaacacatcc tttaaatatt ttgtaaatac atcataaacg
    11641 attttgtaat cgttttcaag agttatgtga tactttttta aattgtgtca tactaatgaa
    11701 ttttaacccg aaaatatgtt cgtgtttttg ttagggatgt ggtaaaatgt gagtattaaa
    11761 ggagaacaaa tatgacaggt aaaacacatg cttcatgcgg catgctcgtt ggagcgctaa
    11821 caacacaata ttttcaaacg gatatttttt cgtcggttac ggttatcatt ctagcaactc
    11881 tagctagctt attacccgat atatgtcaca ctcaaagtaa gattggcaga aggtttaagg
    11941 taattagttt ttttgtgaga ttgatctttg gacatcgaac ctttacgcat tcaatattat
    12001 ttatcgcaat catagcattt ttattgcaaa ttattcagac gcctaattat tatatggcag
    12061 caattataat tggattagtg tcacatgtta tactagatat gataacacca aaaggcgtga
    12121 aattatttta tccgttaccg tttaatgtga agacaccgat tcaatttaaa acaggtggac
    12181 ttgtagactt atctttagct acagcgttaa tggtcggaac agtttatgtt ttatttcagc
    12241 ctttcatcaa tgatataata agcaactgga atactaagtt tttatagaaa ataaattatt
    12301 aaaggttaca aactcagcag gaggcataaa atgctagata aaaatcaatt agctaaatat
    12361 aaacaagatc atttgtgtga atatgaaaaa ataatgagta acaatgaaaa agaagcactt
    12421 gaagagaagg tagcgtcatt agatttagat tttattgcca aattatataa cgatttatac
    12481 atcaataaga aaacaataga tgatgtatct gcagtttctg aagtgaaata tgatattaaa
    12541 tcacaaatga gtgatgatga gattaagcgt ttagaggaac aaggacttca agctattaaa
    12601 gaaggacaat ttgcagtact tttgatggca ggtggtcaag gaacaagact tggttacaag
    12661 ggccctaaag gttcgtttga aattgaaggt gtaagtttat ttgaacttca agctaaccaa
    12721 ttaaaaacat tgaatcatca atctggacat acgattcaat ggtatattat gacaagtgat
    12781 atcaatcatg aagaaacttt agcttatttc gaagcacata gttattttgg atatgatcaa
    12841 gaagcaatcc atttctttaa acaagataat attgtggcgc ttagtgaaga agggaagtta
    12901 attttaaatc aacagggtcg tattatggaa acgccgaatg gtaatggtgg cgtgtttaaa
    12961 tctttagaca aggcaggata cttagaagaa atgtctaata atggcgttaa gtatattttc
    13021 ttgaataata tcgacaatgt tttagtaaga gtgttggatc cactatttgc aggatttacg
    13081 gtagaacatg attacgatat tacttctaaa acgattcaac caaaaccagg agaaagtgtt
    13141 ggacgtttag ttaatgtcga ttgtaaagat acagtactgg agtattcaga attagatcca
    13201 gaagttgcaa atcaatttaa caatgctaat atcggtattc atgcatttaa acttggtttt
    13261 attttaaacg ctgtaaatcg tgagttacct tatcatttag ctattaagaa tttgaagcaa
    13321 ttagatgaga actttggtgt tattgaacaa ccaactttaa aattcgaatt gttctatttt
    13381 gacatattta cttatggaac tagttttgtc acgttacaag ttcctagaga agaagaattt
    13441 tcacctctta aaaataaaga aggtaaagat agtgttgcaa ctgcaacaga agatttacgt
    13501 agaatgggtt taatttaatg ataggcggtg actatgatgt ccaaatcaac taaaaaaagt
    13561 aatgacacaa tcgtagaaac atttaaggat atcatccctt tatcatttgg agaagaaatt
    13621 ggtaatgcgg catctcatgg tgttgcagct ttgttaacat tattagtgtt accctatgcg
    13681 gctgtgcaca gttatattgc acatggtacg cttgcgtctg tgagtatgtc tatttatgtt
    13741 atttctatct ttatgatgtt tatttcatct actatttatc attctatgca aaatgaaaca
    13801 cctcataaat atattttaag gattatcgat cacagtatga tttacgtagc aatttctggt
    13861 acatatacgc ctattttgtt aacagttgta ggtggttgga taggttggac agtatttatt
    13921 ttattatggg gaacgacact ttgggggatt ttatataaat ccatagctgt caaagttaac
    13981 caaaagttaa gcttaattgt ctatttagtg atgggctggg taggtattat cttgttacca
    14041 attattttta ttcgcacatc atggatattc atattattca ttttcttagg tggtgtgtct
    14101 tatacaattg gtgcatggtt ttatgcacaa aagaatagac catattttca tatgatttgg
    14161 catattttta ttgtaatagc atcactattg catttaattg caatcttata ttttatgtag
    14221 tcgtttagaa aagctggggg aaactccgct ttttttatta cataaatcat tctaataggg
    14281 gattgtattg taatagaatt atc
//
LOCUS       DAKTGS010000071        14006 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.071, whole genome shotgun sequence.
ACCESSION   DAKTGS010000071 DAKTGS010000000
VERSION     DAKTGS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.071"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(72..2030)
                     /locus_tag="O0286_001691"
     CDS             complement(72..2030)
                     /locus_tag="O0286_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0170070.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(2036..2956)
                     /gene="pfkB"
                     /locus_tag="O0286_001692"
     CDS             complement(2036..2956)
                     /gene="pfkB"
                     /locus_tag="O0286_001692"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCY0170071.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(2953..3717)
                     /locus_tag="O0286_001693"
     CDS             complement(2953..3717)
                     /locus_tag="O0286_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0170072.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(3967..4449)
                     /gene="ybaK"
                     /locus_tag="O0286_001694"
     CDS             complement(3967..4449)
                     /gene="ybaK"
                     /locus_tag="O0286_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCY0170073.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(4623..5075)
                     /locus_tag="O0286_001695"
     CDS             complement(4623..5075)
                     /locus_tag="O0286_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170074.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(5368..6534)
                     /gene="norA"
                     /locus_tag="O0286_001696"
     CDS             complement(5368..6534)
                     /gene="norA"
                     /locus_tag="O0286_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCY0170075.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            6743..7165
                     /locus_tag="O0286_001697"
     CDS             6743..7165
                     /locus_tag="O0286_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0170076.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            7351..7968
                     /locus_tag="O0286_001698"
     CDS             7351..7968
                     /locus_tag="O0286_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCY0170077.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            7965..8249
                     /locus_tag="O0286_001699"
     CDS             7965..8249
                     /locus_tag="O0286_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170078.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(8404..9777)
                     /locus_tag="O0286_001700"
     CDS             complement(8404..9777)
                     /locus_tag="O0286_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCY0170079.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(9863..11416)
                     /locus_tag="O0286_001701"
     CDS             complement(9863..11416)
                     /locus_tag="O0286_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCY0170080.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(11699..11986)
                     /locus_tag="O0286_001702"
     CDS             complement(11699..11986)
                     /locus_tag="O0286_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170081.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(12021..12929)
                     /locus_tag="O0286_001703"
     CDS             complement(12021..12929)
                     /locus_tag="O0286_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCY0170082.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            complement(13032..13958)
                     /locus_tag="O0286_001704"
     CDS             complement(13032..13958)
                     /locus_tag="O0286_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0170083.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
BASE COUNT         4593 a         2612 c         1965 g         4836 t
ORIGIN      
        1 ctaacatata ttcattgaac atacaactta atatgaagct ctttgctaac aatcatttta
       61 catcattaaa actactcgtc cattgatttt gaagcttcga tttctgtttc agttaacttt
      121 ggtttgatta aaccgtaaat taatgctgaa actaatgtgc caactagaag tgcaataaga
      181 gtttgaagta agtgtgcacc atcagtacca acaattacaa taataccacc atgtggcgca
      241 gtaattcgtg aacctaagcc taaagcaatt gcgccaccta tacctgaacc aatcatcatt
      301 gaaggaataa cacgtaatgg atcggcagct gcaaatggaa tcgcaccttc tgtaataaat
      361 gacataccca tcacatagtt agggataatt gaaccacgtt gttcttttgt aaatttacgt
      421 ctaaaaatta acatcgctgt cgcaattgct aacggtggaa tcataccacc aatcattgca
      481 gctgtaattg gtgctgcatt accttcaatc aacgcacctg ttgcaaaaac atatgccgct
      541 ttgttgaatg gaccgcccat atcaatcgcc atcatagcgc caataactaa acctaataat
      601 acaatattag aacctgataa attgtttaat ccatctaata acaaatgatt taaccaagat
      661 gctggtggat taaaggcata aatcatcaat aagcctgtag ccgtcactgt taatagtgga
      721 taaattaatg ttggctttaa tccctctaat gcttgtggta acttacgtgt catggcttta
      781 acaccttgtg ttaagtaacc tgctaagaaa cctgcaataa taccaccaat aaatcctgaa
      841 ccacctgaaa ttgctaacat accacctaca agacctgaag cgaaaccagg tttatccgca
      901 atactacgtg caatgaatcc agataaaatt ggaatgatta acgcgaatgc acttttacta
      961 ccaatgttcc aaagctgctc tgcaaacgca ttgtactctg agctttttgg attaaatgaa
     1021 tttgctccaa ataaaaatac aattgccatt aaaataccac cagatattac aagtggcaac
     1081 atgttagaaa caccgttcat taagtgttta tagaatgctt tacctgggct taatttttct
     1141 tgactgtcat ttgagttacc ttttctttga ccatcacggg caacaaaagg tttacgactt
     1201 gtatctaatg ctttattaat taattcttct gggcgtttaa taccatctgc tactggtact
     1261 tctacgacat ttttaccatc gaagcgatcc gtctcaacat gaacatcagc agcaacaatg
     1321 atacctgttg cattttcaat atcttgttca gttaaatggt ttttaatgcc gcttgaacca
     1381 ttcgtttcta ctttaatttt aatacccatt ttatccgctt gctttttcaa tgcatcacgt
     1441 gccatatatg tgtgtgcaat acctgttgga caagcagtta ctgctaacac atatggctca
     1501 ttagattgtt tagatgattg ttctgcagtt gcaacttgtt gtgcttcagc ttctgcctct
     1561 tcttcttttg tcacttcatc atcagcctca tctatgatcg ctagtacttc ttcaggtgat
     1621 gaagcatgta ataatttctc acgtacattt tcatccatta aaataccaga caacttagct
     1681 aaagcatcta gatgtgtttg ggcgccacct tctggcgctg caatcataaa gaataagtgt
     1741 gctggttgca tatccaaact ttgataatct acgcctgctt tagatttacc aaacgcaata
     1801 gctggtgact taactgcggc cactttggca tgtggaatgg caataccttc gccgatacca
     1861 gttgtacttt gtgattctcg attgtgaatc gcttccttaa atgacgcgac atcacttaat
     1921 ttacctgctt tgtctaattg atttactaac tcatcaataa caccattttt gtcatttgcc
     1981 attaaatcca ttgctattgt atcttttgtt aataactctg ttactctcat tattttcact
     2041 ccccatcaag tacgctaatc gtaacttgtg attttatttt ttctatagcg tcccgtgttg
     2101 ctaagtcctc atcaaatgcc gtggcagtac cgcatgcgac tgcttgttgg aatgcttttt
     2161 caatcgttaa acctgaagca attccagcca ccatgcctgc aactgtacta tcaccagagc
     2221 caactgtatt aaccactttc ccttgtggat taactgcttt aatactgatt tctttatcaa
     2281 tataaatagc accatcaccg ccaagcgaga caataacaga ttgcgcacct ttatcaacta
     2341 acaaacgacc atatttaata acatctgtgt ctgagttcac tgttgtatta aacatcactt
     2401 ctaattcatc tttattaggt ttaataaata gtggatgata tggtaaaacg ctttcagcca
     2461 attctttttc agcgtcgact actaatttag cacctgtctg tgctgtaatt tgtgcaattt
     2521 gcgcatacgc atcgcttgga atactacttg gtacacttcc agcaacaata actatatctt
     2581 cgcttgttgt atttttaatt tgttgtaaca gttgttcaaa ttgtgttgac gttatatgag
     2641 gacccggtgc attgatttct gtttcttgtc ctgtttttaa tttcacatta atacgtgtat
     2701 cttcatcaac ttcaataaaa ttcgattgaa ttgcactgtt atttaatgta tctataatga
     2761 atttcccagg aaatccacct gcaaatccca aggcagttga ctcaacatcc aatgtcttta
     2821 agacgcgcga gacattaata cctttccccc cagcgaattt atatgttgct gttgctctgt
     2881 tcaaaccatc aattttaaaa tcattcgtaa aaatgacata gtcaattgaa ggattgaaag
     2941 tcactgtata aatcataaag tccctcctat aaagtgatac ttttgttggt attcttttaa
     3001 cgattcttga tttaatgctt tttcagatgt gatgattgtc gtactttcta gcaaaggtac
     3061 acgagcaaaa tatactttat taaacttaga atgatctata agtacaaatg attgattggc
     3121 taatgacatt gctgtttgtt taactaatgc ctcttgctca tcgggagtag ttaatccaag
     3181 ttcaatatct aatccattca tcccgataaa agctttatcg aaacaatatc gtcttaatat
     3241 ctccatagca ctagaaccaa tcgtagcaag tgtattttct ttaacttgac cacctagcat
     3301 aattgtttta ataccttttt taagtaaagc ttctacatgt gttaaaccat tagttaccac
     3361 aatgatatct ttcgcttgaa tatatttaat tagctccaat gtagatgaac cagcatcgat
     3421 aaataagcat tcattatcgt tgatttgatt agctgctatt ttagcaatca ttttcttttc
     3481 atcaagattc gttgctaatt tttcagttaa attcgcctca accatacgat tttcttttaa
     3541 cattgcacca ccatgcacac gttgcagttt ccctaattgt tgtagtttag ataaatctct
     3601 tcgtattgtt gaagcactgc aaccagttcg atctattaat tcttgtaaag tcaaaaaatc
     3661 tttgtgcgaa agttcttcta atattaactc gtgtcttttt tcagtaatta tcatcatcac
     3721 cccatctcac tttgtattat aatgagtacg ctttcacttt tcaatcataa attaccaaaa
     3781 acaatcacaa acactcaaat tgtgacgata aaattacatt tttatgttca aatactcaat
     3841 tataatgact gtttatgatc gtaaaatgct aattcaatca aaaaggagtg tggcagaaat
     3901 gaattttata aaaatttcat ctctgtcaca ctcctaatct tgccccatac tttttgttgt
     3961 tattaatcat tcatggataa ctgctccaat tttgccttta gttattgtaa tcaaatcctc
     4021 aacagctatt gtgatttgca ttgttcgaag cccaccactc acactgatat gactataatt
     4081 ttcacacgat ttgtcaacga ctgttggaaa caatgttttc ataccaacag gcgaacaccc
     4141 accacgaatg tatcccgtta catttttcaa attatctaaa ggcataagct gcaatttctt
     4201 ctctccaacc aaagcagctg cctttttcat atctaaagtt tcacttactg ggataacaaa
     4261 tacaaaatgt tcatgttttg tattttctag aacaagcgtt ttaaatactg ttttaacatt
     4321 tgcttgtatg agttctgcga cttcttgacc agataaatgt tcttctggta cctcaaagct
     4381 atgaacttcg tatttaattt ttgcacgatc aagcatacgc atcgcgttcg ttttcttatt
     4441 tttagccatt tcaacacctc atttatactt cttcagaact atattaacat tttaaaatac
     4501 attacggatt ggttgatttt gacataacat ttacatcatt ttataagttg aattcacctt
     4561 tttaaattaa attattgcaa aataaaaaca gtatatccaa ttagaataca ctgttccatc
     4621 tattatttcg ttattatgcc ttgatttgaa atacttacat gttgcttact agttgaatta
     4681 tcattggaat acacataatt atatccatga tttgtcttag aaattctttc taaaccataa
     4741 ttatcattga tatgcttatc tgcataacga tcagcaataa cttggcactc ttgttcgtta
     4801 aaagaatttg atttatttgc attcacacga tgtttaaaat gattttgctt tttcacattt
     4861 tcattcactt cagatgcttt agcagtatga atattgagaa tatccatttt ctttttataa
     4921 gtatcttcag ttgatacatt tatcgcgtta agcgtattaa ttgttttatg taccttagca
     4981 agcgcatctt catttgttgc taatattaat aaaccaatta aacaaacggt taatattaca
     5041 gcaacaaagc ctaaaaataa attttttact gtcatcccct tacccacatt tccttactca
     5101 ttttttatca tcttaacaca ttaacgttaa tgaattaggt atgtggattg caatttaaaa
     5161 agaatgtaat atttgtaaca tgtttattac tcaaaatcat tttttgttac atataacatt
     5221 taaatataaa gagtaaaaca tttagtcaat cgaatctttt taaatgtaaa gcatcgaatt
     5281 ttcttaataa aattcataag aaaaacgatg ctaatcattc ataatattac gctttacttt
     5341 atatcaatat acaccaaaat acttatgcta catattttgt tctttcaatt ttgctctatg
     5401 ttgcttttca attaaaacaa taacaacacc tgctaatgaa acacctatag ccatataaat
     5461 tggtgcttca atgtgtacat caaataacgc acctgcgatt aaaggaccta tgaaattacc
     5521 catactagtg aatgtcgagt tcaatccgcc tgcaaagcct tgcctttctc cagcaatatt
     5581 agaaaaataa tttgtaatgg ctggtcgtat catatcaaaa cctatgaaga caacaaaact
     5641 gattaacatt attgaccaat agtcattagc aaaaactaat aatattaaga caacaactga
     5701 atataataat gaccaagcta taaatgttaa ctctgagaaa tacttcataa atttatcgaa
     5761 gaaatagatt tggaaaagtg ccccaaatat accgccaccc gtaatagcaa tcgaaatatc
     5821 tttaggtgaa taatttacct tgtcagctgt gtatagtgaa tacaatgttt caaatgcaga
     5881 taaaccaaac gataatacaa gtgttaaaat aactggtgta ataaacactt tccagttaat
     5941 tttcgttagc aattgtggct ctaacttttg gaaaccactt gtcgtagact ttttcggatc
     6001 gtgaatcaat acaattgaca ttataaatgc tagaatacct aatgctcctg caaagtaaaa
     6061 tggcatacga tgtgaaactt ctgccataaa tccaccaatc cctggtccta aaatgaatcc
     6121 agaattgata atcgctgaca tgtagccaaa gttttttgct ttttgatggc ttggtgaaat
     6181 gtcagctatt aaacctgtca caccaggcat taccatacca gcactcatac caccaatcac
     6241 tctcgataac atcaataccg aaaaattgtg gccaactgca aacataaatt ctgacactga
     6301 aaacaaaatt aatcctatac atataattaa tttcttccct aatttgtcag ctagcgtacc
     6361 accaaacggc gatataatca tttgagataa cgcaaaagca gcaactagta atcctaaatc
     6421 actaccagtt aatcccaaat cttttaaata aacaggcaag actggtatta ctaaaccgat
     6481 acctaaaaaa atcaagaaaa tattaaaata taagacaaaa atctgtttat tcatatgctc
     6541 acctcttttt tctccatgtc atgcttaaag ctgttattct tcttttccac gacagattgc
     6601 aattcatgct aaatattcgt aaaaagtttc tatattgtat tacaacattg ctacatttga
     6661 caatattttt tctttgtaat tatcactatt ttccatttaa ttgtataatt aatgacatat
     6721 taaaataaag gggattgatc atatgaaatt gcaaaaaagt aatcatacta tactactagt
     6781 gttggaaaaa ggagaagata tcgttgaatg tataacaacc tttgcagatg accaagactt
     6841 aacatttaca tctgtcagcg gcattggtgc atgtgatgac gttgtattaa aatttttcaa
     6901 tttaacaacg aaacaatacg aagaaaaaca tattactgag ccactggaat tgacgagctt
     6961 attaggtaat atttctcgtc tagacaatgg tcattttgca catttgcatg ctactttcgg
     7021 tactcaatca tatgaaactt ttagcggaca tctcgctaaa gcaattgttt ctgcaactgc
     7081 tgaaatcatt cttactgtaa ctgatttgga cattcaacgt tcattcaaag atgcagtcgg
     7141 tttaaattta cttgatcctc aatagcactt tactttttag ttgaatcaaa ttttattaca
     7201 agttatcata agtgcttaat cataaatgca ttctcgttta ttaatatact tcaacgagtt
     7261 aaattaaatt tttaattagg aattgaatgc gtttataact atcacatttg aatgaataac
     7321 tttttcatta gaaagtagtg tttattattg atgcaatctc gatacatcgc tagaatttac
     7381 tttttaattt tatttatcgt ctcccttttt gagactaaaa tttttcaatt tatgacttta
     7441 aatttgtttt tagcatatat accttttgag cttagtctat tattacgatt attcaaaccg
     7501 aataaaaagt atgaatggcc actctttatc atatatagtt ttatatttgt gttactttta
     7561 ccaaatacat tttatatggt aactgattta atccatttga accaatttca attcaacttt
     7621 tacgcaggac taaatttaag tgagtggaaa tactttacct atttactgct tggtgtcttc
     7681 ttagcggttt atgtgatgat tttaatctat tttgaaatac taacattgac ttctcaccgt
     7741 tggcttaata gattaattgt tgtcgtgcta atgtttttaa atggattcgg aatttatatt
     7801 ggtcgtttct tacgcttaca ttccgtttat ttctttaatg agcccttaca tgttctaaat
     7861 cacattctag aaagtctaac gcttaaaact gctatgtttg tatgttttat ggttgtcatg
     7921 caagcagcaa ttctattatt tggaaaagga gtgcgtttga acaagtgaca ataaatataa
     7981 tcgttttaat agtatctatc attgtctttc aattgattat tggacacata tggcatgata
     8041 ttggtttaag ttatttaaga agtatcttac tcatgatgct gccatttgga ctaggtgtat
     8101 ttatacaaca agtgtcgtat tacgaacgac aatatcctaa atggcaagtg cctcaaaaca
     8161 taaaagttag attgaaatat atttatctcg ctacgttttt agagtatgtc gtactatacc
     8221 tcactttatt tacggacata ctaagataac atcaaattaa taagaaaact aaatacaact
     8281 tatatatttg aggaaagcca atttctatca catcaataat agaaattggc ttatttatat
     8341 tgcttaagaa ctttatattc tgctccagat tttactcatg ccagatctat caattgagcc
     8401 gaattagtct agcgctttaa atgtagctaa aacttgtgta cgtttttctt gatgatctac
     8461 catttgtctt ggataatgac tacctaattc aatcccctgt tcaaaaaggt tggactcatt
     8521 gcgttgtgta tcatgcaaat attttgcatc aatttgatta aaaatcggaa gatatgtttt
     8581 gatatacaaa gctttagcat caaagcgttc actctgtctt attggattaa acattctaaa
     8641 atacggcact gcatccgtac ctgtagaagc agaccattgc catccatgaa tatttgatgc
     8701 tgcatcatag tcaataaggt gctttctaaa gaatttttct ccccatgtcc aatctataaa
     8761 taaatctttg gttaaaaatt gcgacacaac cattctcatt cgattatgca taaaaccagt
     8821 ttgtgtcaat tccattattg ctgcatcaat gattggaaat cctgtttgcc cttcgcacca
     8881 tgcattaaaa tccgcttcat tttgcgacca ttttatctgt cgatatttag gtttgaaagc
     8941 ttggtatgag gtttcaggat actgtgtcat taacacataa taaaattctc taaaaatgag
     9001 ttcacgtata aatgcttcat agtttgcctc atcttcatca taacgcgcca ataaatcatt
     9061 aaaaatttca ataatatcta ataatccata tgctaaagca acacttagct gacttgttaa
     9121 tacttcaggt aaatagtctc ttccgctttc gtaattctgt atatcttgat ctaaaaaatg
     9181 ttgccattta ttctgttcaa ttatttgtga ggatccaaat gctatgtcat caaacactaa
     9241 atcatcaggt gatgcaatga caaagtcttc gaatgatttt aaatcatagt gatatacgtc
     9301 tctatgcctc aagtaaggtc gccatttttt atagaaacta gtaaaaacat tgtatggctc
     9361 cccttgttga ttcattgttt tactcgcttt aaagtaatga ttcccacgta tcaaagtaac
     9421 ggcaatgcgt gcttcattga ataaagaacg ttgatgtaaa atatcatatt cttcaagatg
     9481 ataactcata atatcacccg cagtcactac ttcagagata ttgttttctt tacaaaatga
     9541 aatgatatcg tcataagaca taacataagg tgtaagcttt tctgcgtgca acgattggta
     9601 aaagcgctgc aaagtaccgc gataaaactg tgctttcaaa tcagaagctg aagacatgtc
     9661 ctcttccgga atgataaaat aacatgcatc aatgtcttct ttttgttgat aaatatattc
     9721 aaataatgga ttgtgttcca ttcgaaacat tcgatttaat aacacagcaa ttgccataaa
     9781 caaaacaccc ttcaattatc tttttatttt attaagcttt ttgtcacttt aaaataacct
     9841 agattcttat ttatattttt aattaaagca aacctaacca tttccaataa gtcatgctaa
     9901 aaactatcac tagaatatac cctacaattg tcaatggtat acctgccttc aagaaatctt
     9961 taaccgtaaa agtaccagtg ccataagcca acatattttg aggtgcacta acaggtaata
    10021 agaaaccaaa actaataaca aattgttgaa ttaaaacaaa tcctatagac tggtctccta
    10081 agtgtaacgt agaggttagc gaaataaaaa caggtattaa cgctgatgat aaacttgttg
    10141 cactcgcaaa gcccaaatga atcaatatat taaaaagcgt gataagcgca attgtcgcga
    10201 taataggtaa atgttttaaa cctaaaacac caaaagtttg atcacttaac cattgagctg
    10261 cacctgtttt caaaagaacg ttacctagtg aaatacctac accaaacacg ataattgttc
    10321 cccatggtat tttattttca acatgtttcc atgtcatgac accaattttc ggcattaaca
    10381 taacacctaa agcaataata gtaatggatg cagagtcaat cggatgtaat actttttcag
    10441 ttgaccaaaa cagtaataat aacatcgata taacaattaa acgccattca cgtgggctaa
    10501 cggggccaag tttatgcaat tcttctttta ttaaatcttt accaccttct attgtattaa
    10561 tttctggagg catcacttta atcatgatga aatataaagc tacggacata actatggacc
    10621 aaggcgctgc atataagaac cactcgcccc atgaaacatc aaatcctaat tgatggttta
    10681 taaaattaat cgctacgata ttttgtgctg ccgccgtttt gataccaata ttccaaattg
    10741 acacagcttg tactgaagta attattaata aagacgctaa cttgctatct ttggaaactt
    10801 taaatgccgc aatcataccc agcaagattg gtacaactgc ccctgctcta gctgttgcag
    10861 aaggaacgaa aaatgcaagt acaattgaaa cgataattgc tccaataact atatttctag
    10921 ttttattacc aacaattgat aacactaaaa gagctagtct tttatgcaaa ttcgtttctt
    10981 gcatagcagc agccaaaaat aatgcagctg caacgagagc tacagctgaa gttgcaaatc
    11041 cactaaacgc taatgataat gcatgattag ttcctagaag gtcacttcca gctaaaatag
    11101 cactgccact tttcggatta cctagcttct cccctaaatt ttgaacaggg ctaaatccta
    11161 aaagtaatat cattaagcca ataattaaag ttgctgacac cggatatgat acagcttccg
    11221 ttacccacat aataactgca aaagctaaaa tagctagtac tgccttagcc attatcggca
    11281 gacttgaagg tgccgggagt aatagtacaa ctataagtat tataaaactc atgacaatcc
    11341 aaaatggctt gtattgcttt tttgtctttt ctttcttttg ttgttcatgt tcaactacat
    11401 gtttttcttc tgacataaca gtcacccctt tgtttaatct taatttaatt ataacaattt
    11461 tttaaaacat acatttttaa tttatgcaaa atacacaatt ttatcgaaat attttgtaaa
    11521 taatattgct aggtgatgag ttatctcggt taattacaaa acgatatcta agtttaaata
    11581 ttagatcata aaaaaactat ctactgaata tcctcatagc atcatttttc taagcaaaag
    11641 ttaaacttag ttagataaaa tgcaaatgag attattgtag atagtctctt tttaatgttt
    11701 aaaaatgatt aatgccataa acataaataa ttccaaagaa tgccgcgcca ctaagcactg
    11761 ttaatataag acttagtaac aatgtgcgtt tataatgttt aacaaatgct atgaacaaaa
    11821 tgactacatt atacgcaaag aaaagtccga ataatgtcat tgttattttc aaatcagaat
    11881 agaaaatatt taatagtaat acaatgactg cataaattaa atacggaata actataaatt
    11941 tgcgttcaaa tcttaattgt tcgaaacgtt tatcttcgtt tgtcatgata tgctccttta
    12001 tgatttaaaa gtaaataagt ttacggaata tcttgtacga taatagcggt gtaaatgtca
    12061 aaccacgact gatcatctaa attaagttgt agcccttcga ttgcttgatc aatacgcttt
    12121 aactgacttg ttccaagtat cggcatgata cgatgcggta tttttactaa ccacgctatc
    12181 atcacagctg tgtcactcac accatatttg tcagctattg attgaacaac tttcataata
    12241 cgttgcgctt taatatcttc cttgtcgaaa attttaccgc ctgcaaaagg actccaagcc
    12301 atgatttgaa catggttttg atacattgaa tccattgttc catcttgtaa actatcaacg
    12361 tgatatggcg ataattctaa ttgattgatg ctaatatgta gtctttcttt cataatatat
    12421 tgatttaaca attggtattg tgaatgatta aaattcgaca ccccgaatga cttcaactta
    12481 ccttgtttaa caagtttagt taatgcatca gcaacttgtt ctggatccat caatggtgaa
    12541 ggacgatgaa tgagtagact atctaaataa tctacattca aattgattaa tgactgttca
    12601 acagatttca cgatgtgctt actactcaaa tcataacgat gtccatttgt aaaatcaaat
    12661 tgcttagaag gcaaaatgat accacatttc gtaacaattt gaattttatt tcttaattcg
    12721 ggtgataaat ccaaagcatt accaaacagt gattcacatt gataatctcc ataaatatca
    12781 gcatgatcca tcgttgtaat tccacgttca actaattcat ttataaaata atttaactct
    12841 ttcgcagtca tcttccattc atttgcacgc caaaaacctt gtacaagcct agaaaaatga
    12901 acatagtgat taatcattat ttgttccata tctcatccac tccatccctc aatgtcacaa
    12961 aatattcaca ataaaagtat taagaaatta atgtgctatt tccgttaaat tgaaacaaca
    13021 cattttctga tttacgtaaa ttgtagcata tccaattgat tacgtatgtg atttgtatct
    13081 aaagtttcac caataataac aatcgttaat ggtaattgca tgccaattat tccatagtct
    13141 ggtaaaccat atgcatattg aaattcataa attgcatttg gttgatctct aaatgacaca
    13201 taacctttca aacgtagaac agattccggt aatttcatta taaattgata aaacaaatga
    13261 cgatcaatcg gacctgtaaa cgtgtaagtc atacttttaa tcccatgatg atggtgatga
    13321 tgagacgata tctctctgtc tttaacagtt aagtccaatt cttccaacga aacttttcca
    13381 taggttgttt tataagttgg aatacttgca caaatcatac ctaattgctt atcaattttc
    13441 tcaagactgt catcagttat aagatcaatt ttattaataa taatcatgtc acttagtttc
    13501 aactgatctt ccatcagcga aactgtattt tcggtatatt gatgacgttc taaaaatcga
    13561 gtcgcatcta atacaccata aataatcggc ttttcaaaga aattaacgat ttgcggatct
    13621 tgacatgcaa ctagtaattc caaaggatgc gcaatgcctg tcgcttctat gatgacatga
    13681 ttaacgtccc cttttaaagc aatggctttt agttcataga caagttcttg tttaagatcg
    13741 caacaaacac aaccattaat caatgaatgg acttcaattt catttgaaat actattgcta
    13801 tcaatatcaa aagtaccgaa ttcattcatg atgattttaa ttttctcatc attttttaat
    13861 aattcactaa tataatgtgt cagtaacgtg gttttaccac tacccaaaaa tccgttaatt
    13921 atggatattc ttattttttc atctttatta tttttcattt tatattctct tttctattac
    13981 agttattttt aaaaatgata acatat
//
LOCUS       DAKTGS010000072        13958 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.072, whole genome shotgun sequence.
ACCESSION   DAKTGS010000072 DAKTGS010000000
VERSION     DAKTGS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.072"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            736..2031
                     /gene="purB"
                     /locus_tag="O0286_001705"
     CDS             736..2031
                     /gene="purB"
                     /locus_tag="O0286_001705"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCY0170084.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            2140..2442
                     /locus_tag="O0286_001706"
     CDS             2140..2442
                     /locus_tag="O0286_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170085.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2614..3306
                     /locus_tag="O0286_001707"
     CDS             2614..3306
                     /locus_tag="O0286_001707"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCY0170086.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3303..5495
                     /gene="pcrA"
                     /locus_tag="O0286_001708"
     CDS             3303..5495
                     /gene="pcrA"
                     /locus_tag="O0286_001708"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCY0170087.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5499..7502
                     /gene="ligA"
                     /locus_tag="O0286_001709"
     CDS             5499..7502
                     /gene="ligA"
                     /locus_tag="O0286_001709"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCY0170088.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7515..8714
                     /locus_tag="O0286_001710"
     CDS             7515..8714
                     /locus_tag="O0286_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCY0170089.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8803..10341)
                     /gene="putP"
                     /locus_tag="O0286_001711"
     CDS             complement(8803..10341)
                     /gene="putP"
                     /locus_tag="O0286_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCY0170090.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10708..11010
                     /gene="gatC"
                     /locus_tag="O0286_001712"
     CDS             10708..11010
                     /gene="gatC"
                     /locus_tag="O0286_001712"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCY0170091.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            11012..12469
                     /gene="gatA"
                     /locus_tag="O0286_001713"
     CDS             11012..12469
                     /gene="gatA"
                     /locus_tag="O0286_001713"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCY0170092.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12482..13909
                     /gene="gatB"
                     /locus_tag="O0286_001714"
     CDS             12482..13909
                     /gene="gatB"
                     /locus_tag="O0286_001714"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCY0170093.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
BASE COUNT         5250 a         2033 c         2581 g         4094 t
ORIGIN      
        1 tagtagctta tttactaaat tgcaaacatc tcattcaata attaatatga tcgaaagttt
       61 catgaaaata tattagtcga ttttgaaaga agcgtagtca atttatgtct ttctataaaa
      121 atttactatt aaaaaaatct ctgcgtattt ttctataatc agtttcgtat ttatacgatt
      181 ctggagttca atattaatgt cttatattta aaatgccaca cctatgcaat agaatatata
      241 ttctttttgt gagtagaaaa tttgttatat tagaagcgaa taattttaaa acatattatg
      301 aaatgtaaga agaaatatta tgaaactagg aaaagtagat tgttgaaaat tccatacaat
      361 aggagaagta aagtgaataa attctggtat tactaaagct attgattgtc ctaattgtaa
      421 taaaaagttg gatagtgtag ttgaggggag aaaaactaaa atgcaaaaat atattctcaa
      481 tggatcatta taaataccaa ttagattaaa gtattgcgaa catttacaat tagaaatcag
      541 tattaatctg ccatagataa ttgtattaat gtaaaaaata aaatagaatg agaatatacg
      601 gaaatttttt atacatttat aaccaattct gttaaactgt gaagagtaga gttaaaaacg
      661 aactttttgt ttatttttat taaaaaatgt tctgtttttg aaaattcgag tatacatacg
      721 ataggagtta aaaaaatgat tgaacgctat tctagagaag aaatgtctaa tatttggact
      781 gatcaaaatc gctatgaagc atggttagaa gtggaaattt tagcatgtga agcatggagt
      841 gaattaggac atataccgaa agctgacgta caaaaaattc gtcaaaacgc aaaggtaaat
      901 gtcgaacgtg cacaagaaat tgaacaagaa acgcgccatg atgttgtagc ctttactaga
      961 caagtttctg aaacgctagg tgaagaacgt aagtgggtac attatggttt aacttctact
     1021 gatgttgtag atacagcttt aagtttcgtt attaaacaag caaatgacat tattgaaaaa
     1081 gatttagaaa gatttatcga cgttttagct gaaaaagcaa aaaattataa atatacattg
     1141 atgatgggac gtacacatgg agtgcatgca gaaccaacta catttggtgt caaaatggca
     1201 ttgtggtaca cggaaatgca acgtaactta caacgtttca aacaagtaag agaagaaatt
     1261 gaagtaggga aaatgagtgg tgcagtaggt acttttgcta acattcctcc tgaaatagaa
     1321 agttatgtgt gtaaacattt aggtattggc acagcgcctg tatcaacgca aacattgcaa
     1381 cgcgatcgac atgcatacta tattgcgaca ttagcattaa tcgcgacatc actagaaaaa
     1441 tttgccgttg aaatacgtaa ccttcaaaaa accgaaacga gagaagttga agaagcattt
     1501 gcaaaaggcc aaaaaggttc atctgcaatg cctcataaac gaaatccaat tggttctgaa
     1561 aatatcacag gtatttcaag agtcattcgt ggttatatta ctacagctta tgagaatgtg
     1621 ccattatggc atgaaagaga tatttctcat tcttctgctg agcgtattat gttaccagat
     1681 gttacaatag cactagatta tgcattgaat cgtttcacaa atattgtgga ccgtttaact
     1741 gtatttgaag ataatatgcg taataacata gataaaacat ttggtctgat tttctcacaa
     1801 cgtgtcttat tagcattaat taataaaggt atggttcgtg aagaagcata cgacaaagta
     1861 caaccaaaag cgatgatatc ttgggaaacg aaaacaccgt tccgtgaatt aatcgaacaa
     1921 gatgagtcta ttacaagtgt tttaacaaaa gaagaattgg atgaatgttt cgatcctaaa
     1981 catcatttga atcaagttga cactattttc gaacgcgctg gattagcata aaattaaata
     2041 aagtaacgat tcagatttaa taaaatataa atcgctacac tttaatagat taatgcgtta
     2101 aaatgacata aagaataaaa ttataggggt tgtttcgata tgcaaattga aaaattacga
     2161 ggtgcagcgt tagatgaatt gtttgatgca atactaacgt tagaaaatag agaagaatgt
     2221 taccaatttt tcgatgattt gtgtactgta aatgaaattc aatcactgtc tcaaagatta
     2281 caagttgcta aaatgattaa gcaaggttat acctatgcaa cgattgaaca agaatctgga
     2341 gcatcgactg caacgatttc tagagtgaag cgttcattac aatggggtaa tgatgcttat
     2401 acaatgattt tagatcgtat gaatattgaa acaaatgaat aataaatgaa cgataaacaa
     2461 tggttatcta tctgcactaa taaggtagat aatcattgtt ttttcacgaa aaaatttaca
     2521 gagtaaaaga acttaaattt catattaagt ctttagaact cgacacttaa aaatgctata
     2581 atcatatgta tgttaaaaaa ggagtttcgg aaaatgtatg acattaaaaa atggcgccat
     2641 atttttaaat tagacccagc taaacatatt tcagatgatg atttagatgc gatttgtatg
     2701 tctcaaacag atgcaattat gattggtgga actgatgacg ttactgaaga taatgtcatt
     2761 catttaatga gcagagtaag aagataccca ttgcctttag tacttgaaat atcaaacatc
     2821 gaaagtgtaa tgcctggttt tgatttttat tttgtaccta cagtactgaa cagtacagat
     2881 gttgtatttc acaatggtac attattagaa gcgcttaaaa catatggaca tagtatagat
     2941 tttgaggaag taatatttga agggtatgtc gtgtgcaatg ctgatagcaa aatggcaaaa
     3001 cataccaaag caaatacaga tttaacaaca gaagatttag aagcatatgc ccaaatggtc
     3061 aatcatatgt atcgattacc ggttatgtat atagagtata gtggcattta tggcgacgta
     3121 tcaaaggttc aagctgtctc agaacatcta acagaaacgc aactttttta tggtggcggt
     3181 atttcctcag aacaacaagc gacagagatg gcagctattg cagatacaat tatcgtcggt
     3241 gatattattt ataaagatat taaaaaagct ttaaaaacag taaaaataaa ggagtctagt
     3301 aaatgaatgc gttattaaat catatgaata cagagcaaag tgaagctgta aagacaacag
     3361 aaggaccatt gttaattatg gcaggtgctg gttcagggaa gacacgtgtt ttaacacata
     3421 gaattgctta tttattagac gaaaaagatg tctcaccata caatgttttg gctattactt
     3481 ttacaaataa agctgcaaga gaaatgaaag aacgtgttca aaaattagta ggtgatcaag
     3541 cagaagttat ttggatgtca acattccact caatgtgtgt tcgtatttta cgtcgtgatg
     3601 cagatcgaat tggtatagaa cgcaatttta cgataattga tcctacagac caaaaatctg
     3661 ttattaaaga cgtcttaaaa aatgaaaata ttgatagtaa aaagtttgaa cctcgtatgt
     3721 ttatcggtgc gatcagtaat ttgaaaaatg aacttaaaac acctgcagat gctcaaaaag
     3781 aagccacaga ttatcactcg caaatggtag caacggttta tagtggatat caacgccaat
     3841 tgtcacgtaa tgaagcgtta gattttgatg accttattat gacaacgatt aacttatttg
     3901 agcgtgtacc agaagttcta gaatattatc agaacaaatt ccaatatatt catgtagatg
     3961 agtatcaaga tactaataaa gcacaataca cattagttaa attattagca agtaagttta
     4021 aaaacttatg tgttgtaggt gactcagatc agtcaattta tggttggcgt ggtgctgata
     4081 ttcaaaatat cttatcattt gaaaaagact atccagaagc gaatacaatc tttttagagc
     4141 aaaattatcg ttcgacgaaa acgattttaa atgcggctaa cgaagtgatt aaaaataatt
     4201 ctgaacgtaa gccaaaagga ctgtggactg caaatacgaa tggtgagaaa attcattact
     4261 atgaagcaat gacggaacgt gatgaagcgg aatttgtaat acgagaaatt atgaagcatc
     4321 aacgtaatgg taagaaatat caagatatgg caattttata tagaacgaat gcacaatcac
     4381 gtgtacttga ggaaacattc atgaaatcta atatgccata cacaatggtt ggtggccaaa
     4441 agttctatga ccgtaaagaa atcaaagatt tattaagtta tttgcgtata attgccaata
     4501 gtaatgatga cattagtttg caacgtatta ttaatgttcc aaaaagaggt gtaggtcctt
     4561 catctgttga aaaagttcaa aactatgcac ttcaaaacaa tatcagtatg tttgatgcac
     4621 ttggagaagc tgattttatc ggattgtcaa aaaaggtgac acaagagtgt cttaattttt
     4681 acgagttaat ccaaagcttg ataaaagaac aagaattttt agaaattcat gaaatagttg
     4741 atgaagtatt acaaaagtct ggctatcgag aaatgcttga acgtgaaaat acattagaat
     4801 ctcgaagtag attagaaaac atcgatgaat ttatgtcagt accaaaagac tatgaggaaa
     4861 ataccccatt agaagaacag tcattaatta actttttaac ggatttatcg ttagtagctg
     4921 atattgatga ggcagatact gaaaatggcg taacactaat gacgatgcac tcggctaagg
     4981 gccttgaatt tccaattgtc tttataatgg ggatggaaga atctttattc ccacatatta
     5041 gagcgattaa gagtgaagat gatcatgaaa tgcaagaaga acgtcgtatt tgttatgtag
     5101 caattacaag ggctgaagag gtgttatata tcactcatgc gacatcaaga atgttatttg
     5161 gtcgccctca gtcaaatatg ccatccagat ttttaaagga aattccagaa tcactattag
     5221 aaaatcattc aagtggcaaa cgacaaacga tacaacctaa ggcaaaacct tttgctaaac
     5281 gcggatttag tcaacgaaca acgtcaacga aaaaacaagt attgtcatct gattggaatg
     5341 taggtgacaa agtgatgcat aaagcctggg gagaaggcat ggtgagtaat gtaaacgaga
     5401 aaaatggctc aatcgaacta gatattatct ttaaatcaca agggccaaaa cgtttgttag
     5461 cgcaatttgc accaattgaa aaaaaggagg attaagggat ggctgattta tcgtctcgtg
     5521 tgaacgagtt acatgattta ttaaatcaat acagttatga atactatgta gaggataatc
     5581 catctgtacc agatagtgaa tatgacaaat tacttcatga actgattaaa atagaagagg
     5641 agcatcctga gtataagact gtagattctc caacagttag agttggcggt gaagcccaag
     5701 cctctttcaa taaagtcaac catgacacgc caatgttaag tttagggaat gcatttaatg
     5761 aggatgattt gagaaaattc gaccaacgca tacgtgaaca aattggcaac gttgaatata
     5821 tgtgcgaatt aaaaattgat ggcttagcag tatcattgaa atatgttgat ggatacttcg
     5881 ttcaaggttt aacacgtggt gatggaacaa caggtgaaga tattaccgaa aatttaaaaa
     5941 caattcatgc gatacctttg aaaatgaaag aaccattaaa tgtagaagtt cgtggtgaag
     6001 catatatgcc gagacgttca tttttacgat taaatgaaga aaaagaaaaa aatgatgagc
     6061 agttatttgc aaatccaaga aacgctgctg cgggatcatt aagacagtta gattctaaat
     6121 taacggcaaa acgaaagcta agcgtattta tatatagtgt caatgatttc actgatttca
     6181 atgcgcgttc gcaaagtgaa gcattagatg agttagataa attaggtttt acaacgaata
     6241 aaaatagagc gcgtgtaaat aatatcgatg gtgttttaga gtatattgaa aaatggacaa
     6301 gccaaagaga gtcattacct tatgatattg atgggattgt tattaaggtt aatgatttag
     6361 atcaacagga tgagatggga ttcacacaaa aatctcctag atgggccatt gcttataaat
     6421 ttccagctga ggaagtagta actaaattat tagatattga attaagtatt ggacgaacag
     6481 gtgtagtcac acctactgct attttagaac cagtaaaagt agctggtaca actgtatcaa
     6541 gagcatcttt gcacaatgag gatttaattc atgacagaga tattcgaatt ggtgatagtg
     6601 ttgtagtgaa aaaagcaggt gacatcatac ctgaagttgt acgtagtatt ccagaacgta
     6661 gacctgagga tgctgtcaca tatcatatgc caacccattg tccaagttgt ggacatgaat
     6721 tagtacgtat tgaaggcgaa gtagcacttc gttgcattaa tccaaaatgc caagcacaac
     6781 ttgttgaagg attgattcac tttgtatcaa gacaagccat gaatattgat ggtttaggca
     6841 ctaaaattat tcaacagctt tatcaaagcg aattaattaa agatgttgct gatattttct
     6901 atttaacaga agaagattta ttacctttag acagaatggg gcagaaaaaa gttgataatt
     6961 tattagctgc cattcaacaa gctaaggaca actctttaga aaatttatta tttggtctag
     7021 gtattaggca tttaggtgtt aaagcgagcc aagtgttagc agaaaaatat gaaacgatag
     7081 atcgattact aacggtaact gaagcggaat tagtagaaat tcatgatata ggtgataaag
     7141 tagcacaatc tgtagttact tatttagaaa atgaagatat tcgtgcttta attcaaaaat
     7201 taaaagataa acatgttaat atgatttata aaggtatcaa aacatcagat attgaaggac
     7261 atcctgaatt tagtggtaaa acgatagtac tgactggtaa gctacatcaa atgacacgca
     7321 atgaagcatc taaatggctt gcatcacaag gtgctaaagt tacaagtagc gttactaaaa
     7381 atacagatgt cgttattgct ggtgaagatg caggttcaaa attaacaaaa gcacaaagtt
     7441 taggtattga aatttggaca gagcaacaat ttgtagataa gcaaaatgaa ttaaatagtt
     7501 agaggggtat gtcgatgaag cgtacattag tattattgat tacagctatc tttatactcg
     7561 ctgcttgtgg taaccataag gatgaccagg ctggaaaaga taatcaaaaa cataacaata
     7621 gttcaaatca agtaaaagaa attgcaacgg ataaaaatgt acaaggtgat aactatcgta
     7681 cattgttacc atttaaagaa agccaggcaa gaggactttt acaagataac atggcaaata
     7741 gttataatgg cggcgacttt gaagatggtt tattgaactt aagtaaagaa gtatttccaa
     7801 cagataaata tttgtatcaa gatggtcaat ttttggacaa gaaaacaatt aatgcctatt
     7861 taaatcctaa gtatacaaaa cgtgaaatcg ataaaatgtc tgaaaaagat aaaaaagaca
     7921 agaaagcgaa tgaaaattta ggacttaatc catcacacga aggtgaaaca gatcctgaaa
     7981 agattgcaga aaaatcacca gcctatttat ctaacatttt agagcaagat ttttatggtg
     8041 gtggagatac aaaaggtaag aatattaaag gtatgacgat tggtttagct atgaatagtg
     8101 tttattacta taaaaaagaa aaagatggat cgacttttag taaaaaacta gatgatagcg
     8161 aagttaaaaa gcaaggtaaa caaatggcta gtgagatatt atcaaggtta cgtgaaaatg
     8221 atgatttaaa agatatacca attcattttg caatttataa gcaatcaagt gaagattcaa
     8281 tcacaccagg tgaatttatc actcaagcga ctgcagaaaa gagtcaaaca aagcttaatg
     8341 aatggcataa tatcaatgaa aaatcagctt tattaccttc ttcaacagca gcagattatg
     8401 atgaaaattt aaataataat ttcaagcaat ttaatgataa tttgcaatca tatttttcta
     8461 atttcacaca agcagtaggg aaagttaaat ttgttgataa aaagccacaa cgattagtag
     8521 tagatttacc aatcgattac tatggacaag ctgaaacaat tggtattaca cagtacgtta
     8581 ctgaacaagc gaataaatat ttcgataaaa tcgataacta tgaaattcgt attaaagatg
     8641 gtaaccaacc acgtgcttta attagtaaga caaaagatga caaagaaccg caagttcata
     8701 tttacagtaa ttaattgtaa attaaagata ataaaaaggt accaatgtta gtcataattt
     8761 tgattaacat tagtaccttt ttaatttgtt aagacttatg aattattttt ctctaacgat
     8821 gtcacgaact ttgtttaagt cagtttcaac aaatgcacca ggttttttag taagtttact
     8881 tacaacatat gtaacgatta cacttacaat aaatccagga ataatttcat ataagccgaa
     8941 tatttcgttg atatgtgcca atggtttaat ccacgcaatc caaacgataa cgactaaggc
     9001 acctgaaacc attccactta cagcaccggc acgtgtcaaa cctttccagt aaagtgcaaa
     9061 tagcacaagt ggactgaacg atgcaccaaa tccggcccaa gcgttaccta ctaagtttag
     9121 aattgtgtcg tttggattcc atgcaatcgc gatggcaaca attgctacaa ctaatacaga
     9181 taatcttcca atcataacaa attctttttg gtgcgtttta gctttttctt caccacgaat
     9241 taatttataa aagtcttccg ttagtgaact agatgttaca agtaattgtg aagaaatcgt
     9301 gctcataatt gcagctagaa tcgcagcaag taagaaacca cctacaagag gatggaagag
     9361 tacttgactc atcacgatga ataatgtctc aggatcttct agtttaatat gataagcagg
     9421 tacgaatgca atacctgtta aaccaacagc cacagcgcct aataaaccaa cagccatcca
     9481 gctaatacct aaacgtctag ctttaggtag catcttgtgt gatttaatag acataaacct
     9541 tataatgata tgaggttgac cgaaataacc taatccccat gaaaatagag agataattcc
     9601 tataaatgat aaccctttaa ataaatttaa atttgtaggt ttcatagctg ctacatcatg
     9661 aaacgttccc cagccgttta aattcatcat agcaacaata gggaccataa ccatcgcaat
     9721 taacataatg acaccttgga agaaatctgt aattgataca gctaaatatc caccaaagaa
     9781 agtatagaaa atgacaatga aagcaactaa tattaaaccg aaatgataat ctaatccaaa
     9841 agcactttca aatagtttac caccagatac gaaaccagaa tgtgtatata atgtaaagaa
     9901 tactacgata atcaatccag aaataatctt taacacatta tttttatcgt ttaaacgatt
     9961 tttaaagaaa tctggtaatg taattgcatc tccagctaat tcggtataaa cacgaagtct
    10021 aggagcaaca acaaagtaat ttatataagc acctaatgtt aaaccgattg taatccacat
    10081 agctgataga ccagtgctat agacagaacc aggtagcccc ataatcatcc atccactcat
    10141 atctgaagct ccagctgata atgcagtaat atacggtcca atactacgtc cacctaacat
    10201 gtactcgctt aggttaccag ttgcttgctt gtaaccgtaa aagccaataa cgattagtat
    10261 caagaagtag acggcaatca taatataagt ttgccaattg gcatctactt gttgacttaa
    10321 tgctgtcccc attgtaagca tgataaacac tccctttatt taatttataa tttagttact
    10381 tgaaaacatt atacataaag ataaatgttt agaaaagtag gaaaattaaa tttattcttt
    10441 ttgaaatact tgcataccaa tatagaaagc gctttcgtat tttttggata tgagcaaatt
    10501 tttataatta aaattgcata atcataatat tgaattgggt tgtctaatat taaaagagtg
    10561 ttgttttcat aaaaaatgat ttgataaacg aatgataatg tttatacagt ttatatgaaa
    10621 ttaaagtagc acctcataaa tacttagatt tttaattgga aatttgatac aatttagtga
    10681 tgaatgactt aaaggaggct tttattaatg acaaaagtaa cacgtgaaga agttgagcat
    10741 atcgcgaatc ttgcaagact tcaaatttct cctgaagaaa cggaagaaat ggccaacaca
    10801 ttagaaagca ttttagattt tgcaaaacaa aatgatagcg ctgatacaga aggcgttgaa
    10861 cctacatatc acgttttaga tttacaaaac gttttacgtg aagataaagc aattaaaggt
    10921 attccacaag aattagcttt gaaaaatgcc aaagaaacag aagatggaca atttaaagtg
    10981 cctacaatca tgaatgagga ggacgcgtaa gatgagcatt cgctacgaat cggttgagaa
    11041 tttattaact ttaataaaag acaaaaaaat caaaccatct gatgttgtta aagatatata
    11101 tgatgcaatt gaagagactg atccaacaat taagtctttt ctagcgctgg ataaagaaaa
    11161 tgcaatcaaa aaagcgcaag aattggatga attacaagca aaagatcaaa tggatggcaa
    11221 attatttggt attccaatgg gtataaaaga taacattatt acaaacggat tagaaacaac
    11281 atgtgcaagt aaaatgttag aaggttttgt gccaatttac gaatctactg taatggaaaa
    11341 actacataat gaaaatgccg ttttaatcgg taaattaaat atggatgagt ttgcaatggg
    11401 tggttcaaca gaaacatctt atttcaaaaa aacagttaac ccatttgacc ataaagcagt
    11461 gccaggtggt tcatcaggtg gatctgcagc agcagttgca gctggcttag taccatttag
    11521 cttaggttca gacacaggtg gttcaattag acaaccggct gcatattgtg gcgttgtcgg
    11581 tatgaaacca acatacggtc gtgtatctcg atttggatta gttgcttttg catcttcatt
    11641 agaccaaatt ggtccattga ctcgaaatgt aaaagataat gcaatcgtat tagaagctat
    11701 ttctggtgca gatgttaatg actctacaag tgcaccagtt gatgatgtag actttacatc
    11761 tgaaattggt aaagatatta aaggattaaa agttgcatta cctaaagaat acttaggtga
    11821 aggtgtagct gatgacgtaa aagaagcagt tcaaaacgct gtagaaactt taaaatcttt
    11881 aggtgctgtc gttgaggaag tatcattgcc aaatactaaa tttggtattc catcatatta
    11941 cgtgattgca tcatcagaag cttcgtcaaa cctttctcgt tttgacggaa ttcgttatgg
    12001 ttatcattct aaagaagctc attcattaga agaattatat aaaatgtcaa gatctgaagg
    12061 tttcggtaaa gaagtaaaac gtcgtatttt cttaggtaca tttgcattaa gttcaggtta
    12121 ctatgatgct tactataaaa aatctcaaaa agttagaaca ttgattaaaa atgactttga
    12181 taaagtattc gaaaattatg atgtagtagt tggtccaaca gcgcctacaa ctgcgtttaa
    12241 tttaggtgaa gaaattgatg atccattaac aatgtatgcc aatgatttat taacaacacc
    12301 agtaaactta gctggattac ctggtatttc tgttccttgt ggacaatcaa atggccgacc
    12361 aatcggttta cagttcattg gtaaaccatt cgatgaaaaa acgttatatc gtgtcgctta
    12421 tcaatatgaa acacaataca atttacatga cgtttatgaa aaattataag gagtggaaat
    12481 catgcatttt gaaacagtta taggacttga agttcacgta gagttaaaaa cggactcaaa
    12541 aatgttttct ccatcaccag cgcattttgg agcagaacct aactcaaata caaatgttat
    12601 cgacttagca tatccaggtg tcttaccagt tgttaataag cgtgcagtag actgggcaat
    12661 gcgtgctgca atggcactaa atatggaaat cgcaacagaa tctaagtttg accgtaagaa
    12721 ctatttctat ccagataatc caaaagcata tcaaatttct caatttgatc aaccaattgg
    12781 tgaaaatgga tatatcgata tcgaagtcga cggtgaaaca aaacgaatcg gtattactcg
    12841 tcttcacatg gaagaagatg ctggtaagtc aacacataaa ggtgagtatt cattagttga
    12901 cttgaaccgt caaggtacac cgctaattga aatcgtatct gaaccagata ttcgttcacc
    12961 taaagaagca tatgcatatt tagaaaaatt gcgttcaatt attcaataca ctggtgtatc
    13021 agacgttaag atggaagagg gatctttacg ttgtgatgct aacatctctt tacgtccata
    13081 tggtcaagaa aaatttggta ctaaagccga attgaaaaac ttaaactcat ttaactatgt
    13141 acgtaaaggt ttagaatatg aagaaaaacg ccaagaagaa gaattgttaa atggtggaga
    13201 aatcggacaa gaaacacgtc gatttgatga atctacaggt aaaacaattt taatgcgtgt
    13261 taaagaaggt tctgatgatt accgttactt cccagagcct gacattgtac ctttatatat
    13321 tgatgatgct tggaaagagc gtgttcgtca gacaattcct gaattaccag atgaacgtaa
    13381 agctaagtat gtaaatgaat taggtttacc tgcatacgat gcacacgtat taacattgac
    13441 taaagaaatg tcagatttct ttgaatcaac aattgaacac ggtgcagatg ttaaattaac
    13501 atctaactgg ttaatgggtg gcgtaaacga atatttaaat aaaaatcaag tagaattatt
    13561 agatactaaa ttaacaccag aaaatttagc aggtatgatt aaacttatcg aagacggaac
    13621 aatgagcagt aaaattgcga agaaagtctt cccagagtta gcagctaaag gtggtaatgc
    13681 taaacagatt atggaagata atggcttagt tcaaatttct gatgaagcaa cacttctaaa
    13741 atttgtaaat gaagcattag acaataacga acaatcagtt gaagattaca aaaatggtaa
    13801 aggcaaagct atgggcttct tagttggtca aattatgaaa gcgtctaaag gtcaagctaa
    13861 tccacaatta gtaaatcaac tattaaaaca agaattagat aaaagataat ttaaatcatc
    13921 aaactatgaa gatttaaaaa ataaaccctt gattgctg
//
LOCUS       DAKTGS010000073        13906 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.073, whole genome shotgun sequence.
ACCESSION   DAKTGS010000073 DAKTGS010000000
VERSION     DAKTGS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.073"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            86..2191
                     /gene="clpL"
                     /locus_tag="O0286_001715"
     CDS             86..2191
                     /gene="clpL"
                     /locus_tag="O0286_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCY0170094.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDSALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(2260..2430)
                     /locus_tag="O0286_001716"
     CDS             complement(2260..2430)
                     /locus_tag="O0286_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCY0170095.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(2443..4437)
                     /gene="feoB"
                     /locus_tag="O0286_001717"
     CDS             complement(2443..4437)
                     /gene="feoB"
                     /locus_tag="O0286_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCY0170096.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(4449..4676)
                     /locus_tag="O0286_001718"
     CDS             complement(4449..4676)
                     /locus_tag="O0286_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCY0170097.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(4892..7381)
                     /gene="farE"
                     /locus_tag="O0286_001719"
     CDS             complement(4892..7381)
                     /gene="farE"
                     /locus_tag="O0286_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCY0170098.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            7526..8074
                     /gene="farR"
                     /locus_tag="O0286_001720"
     CDS             7526..8074
                     /gene="farR"
                     /locus_tag="O0286_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCY0170099.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(8151..8318)
                     /locus_tag="O0286_001721"
     CDS             complement(8151..8318)
                     /locus_tag="O0286_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170100.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(8430..9974)
                     /gene="pruA"
                     /locus_tag="O0286_001722"
     CDS             complement(8430..9974)
                     /gene="pruA"
                     /locus_tag="O0286_001722"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCY0170101.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(10164..10763)
                     /locus_tag="O0286_001723"
     CDS             complement(10164..10763)
                     /locus_tag="O0286_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCY0170102.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            11002..11193
                     /gene="cwrA"
                     /locus_tag="O0286_001724"
     CDS             11002..11193
                     /gene="cwrA"
                     /locus_tag="O0286_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCY0170103.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            11433..13841
                     /locus_tag="O0286_001725"
     CDS             11433..13841
                     /locus_tag="O0286_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0170104.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT         4582 a         2562 c         2226 g         4536 t
ORIGIN      
        1 caattgcaat tgtatatagt ttgcataacg cttcaaaagt aatttctttt ttgtttagtt
       61 caaaaaaatt tagaggtgat gttatatgaa taacggtttt ttcaatagcg actttgattc
      121 aatttttcga agaatgatga aagatatgca aggttcaaat caagtcggaa acaaaaagta
      181 ctatattaat ggtaaagaag tttcacctga agaactagcg caactcacac aacaaggtgg
      241 caatcactct gctgaacaaa gtgcgcaagc ttttcaacaa gcagcacaaa gacaacaagg
      301 gcaacaaggt ggcaacggca attatttaga acaaattggt cgtaacctta cgcaagaagc
      361 acgtgacggt ttattagatc cagtcattgg tcgtgataaa gaaattcaag aaactgctga
      421 agttttaagt agacgaacta aaaacaatcc tatattagtt ggagaagctg gtgttggtaa
      481 aactgcgatt gttgaaggtt tagcacaggc aatcgttgaa ggaaatgtac cagcagcaat
      541 caaagacaaa gaaattattt ctgtagacat ttcatcatta gaagctggaa cgcaatatcg
      601 tggtgctttt gaagaaaata ttcaaaaatt aatcgaaggt gttaaatctt cacaaaatgc
      661 cgtactattc tttgatgaaa tccatcaaat tatcggttca ggtgccacag gaagtgattc
      721 aggtagcaaa gggttatctg atattttgaa acctgcatta agtcgtggtg agatttctat
      781 tattggtgca acaacacaag atgaatatcg aaacaatatt cttaaagatt ctgcattaac
      841 gcgcagattt aatgaagtgc ttgttaatga accaagcgct aaagatactg ttgaaatttt
      901 aaaaggtatt cgcgaaaaat tcgaagaaca ccatcaagta aaattaccag atgacgtatt
      961 aaaagcatgt gttgacttat caattcaata tattccacaa cgattattac cagataaagc
     1021 aatcgatgtg ttagatatta cagcagcaca tttatctgcg caaagtccag ctgtcgataa
     1081 agttgaaact gaaaaacgaa tttctgaatt agaaaatgat aaacgtaaag cagtaagtgc
     1141 tgaagaatat aaaaaagctg acgacattca aaatgaaatc aaatcattac aagataaatt
     1201 agaaaatagt aatggtgaac atactgctgt tgctacagtt catgatattt cagatactat
     1261 tcaacgatta actggtattc cagtttctca aatggatgat aacgatattg aacgtttaaa
     1321 aaatatttct aatcgtttaa gaagtaaaat cataggtcaa gatcaagctg tagaaatggt
     1381 ttcacgtgca attcgccgta atcgtgctgg gtttgatgac ggcaaccgtc caattggcag
     1441 tttcctattt gttggcccta ctggtgttgg taaaacagag cttgctaaac aattagcaat
     1501 tgatttattt ggtaataaag atgcactgat tcgacttgat atgagtgaat atagtgacac
     1561 aacagctgtt tcaaaaatga ttggtacaac tgctggttat gttggttatg atgacaattc
     1621 aaatacgtta actgaaaaag tacgccgtaa tccatactca gtcattctat ttgatgaaat
     1681 cgaaaaagca aatccacaaa ttttaacatt gttattacaa gtaatggatg atggtaattt
     1741 gactgatggt caaggtaatg tcatcaactt taaaaataca attattattt gtacatcaaa
     1801 tgctggcttt ggcaatggca atgacgctga agaaaaagat attatgcacg aaatgaaaaa
     1861 attcttccgc cctgaattcc ttaaccgctt caacggcatc gttgaattct tacatttaga
     1921 taaagatgca ttgcaagata tcgtcaactt attattagac gatgtacaag ttacattaga
     1981 caaaaaaggt attacgatgg acgtttctca agatgcgaaa gattggttaa ttgaagaagg
     2041 ctatgatgaa gaattaggtg cacgtccatt aagacgtatt gttgaacagc aagtacgtga
     2101 caaaattaca gattactatt tagatcatac agacgttaaa catgtggata tagatgttga
     2161 ggataacgaa ttagtcgtaa aaggtaaata acgacacttt aacatatcgc gcatcaaaaa
     2221 tgagcatcag gtcgcccttg cctgtgctca tttttttaat tatttccctg gaaaatgatt
     2281 cgctgtgtgc tgttctgttc cacaacaatc acgattaatg tcacatgtac cacattttcc
     2341 ttgttttgaa cgcttgaaaa attttactag tgtatataag gcatatccga aaattgctaa
     2401 aaaaattaaa atgttaataa tgactgacac tttaaccact ccttaaacaa ataaatgtcc
     2461 gacttgataa aaaatgaatg ttaagacata tgcagtgact agaggatagg caactgcaag
     2521 tgccgtccat ttccatgaat aagtctcttt acggattgct gctactgtag aaacacaagg
     2581 aatatacaat agtataaata tcataaatgc atacgcagat agcggtgtga attgattttg
     2641 aatcacatta acaaggcctg catcacctga tgaatagata atcgccatcg aacttacgat
     2701 aacttctttt gctaaaaatc ctggcactaa cgtagcacct gcttgccatg ttccaaatcc
     2761 gagcggttgc actaacatac caaagaaact accaaccata tgtaaaaaac tttgattgat
     2821 attcacattg ataccatgtg gtcctacata acttaatagc caaatgacta ctgagccgcc
     2881 aaaaataaat gtacctgctt tacgaacaaa gcccttagcc ttttcccaag tactacgcca
     2941 caacgtttta atggaaggca cacggtatgt tggcaattcc acaataaaga ttgcattatc
     3001 attttttaaa atcgtcttag taagtactgt actgactaaa aatgccataa taatacctaa
     3061 aacatacagg cttaatacta ctaaagattg attctctttg aaaaagatac ctacgaacaa
     3121 cgcatacact ggcagtctag cagagcatga catgaatggt gcaattaata tcgttgttaa
     3181 acgctctttt tcattttcaa tactgcgcgc agccataata ctcggtacat tacaaccaaa
     3241 tccgataatc attggtataa aagacttccc gcttaaaccg aacgattcca taatacgatc
     3301 cattaaaacc gcaatacgtg ccatataacc cgaatcttct aataaagata taaagaaaaa
     3361 gagcacaaca atttgtggta caaagactaa tactgatcct acaccagcaa taatgccatc
     3421 tgtaattaaa tcttgtaaaa atggtataac accaagataa ttcataatcg tcttcacact
     3481 atctgtaaat gtaccaccta taaatgcatc gagttgatcc gacaaaggtg tgccaatcca
     3541 tgtaaatgta gtttgaaaga tcaaccacat aattgctaga aagataggca tccctatata
     3601 tttatgtgtt aatatcttgt ctattctaga gctgaaatat tgcttatctt catctggata
     3661 cgttaccacg tcttgcaata acgtctcaat ataatgattg cgtatacgct ccatctctcg
     3721 acgaacagat acagccccta cttgttcagc aacttgatca cgtaaactcg acaatttatt
     3781 tacaacctct gaattaagtt cgtttgcaat ttgcatgtta tttaataaga attgaatcgc
     3841 aataaaccta gcttgatact tatcatgaga tgtctctgtc attattattt gacacatatt
     3901 tttaattgtc tcttcaatct tctcaccata attgatttta aaatgcggtt gatacccttc
     3961 ccctagatgc tttatttcgc caagtaaata ttttgttcct ttgcctgtac gtgccacaac
     4021 tggaaaaata ggtgttttta actttttcat caatttatga taatcgattt ttatcccgcg
     4081 ctttgtagct acatcaatca tatttaatcc gatgtatatt ggttgattaa gttctaacaa
     4141 ttgtactgtt aattgcatat ttctttttag ttgactcgca tcaacaatgt taatgattcc
     4201 tgaaaatgaa tcgtttaata aatagtctgt cactacagtt tcatctttag aaatcggcga
     4261 taaatcatat gtacctggta aatcaattaa ttgtcctaca ttttctttaa gtttccctac
     4321 ttttttctct accgttacgc cactccagtt gcctatatat tcatacgaac cagttaaagc
     4381 gttaaacaaa gatgttttac caacattagg atttcctaaa atacaataat tttccattcg
     4441 tccggctcct attcttctaa tgcaatagaa caagcatcgc aatgtctaat acttaactgt
     4501 tgtccgttta cttcaataat acatggccct ttaaataaac atttttgttt aatcgttatg
     4561 atagcgtcat ctgttaaccc aaaggcactt agacgataca acatattctc attagcaata
     4621 tccattcgct ttattttata agccttattc atttcaccat ttttaatgtt taacatacta
     4681 tttcgctctc ctattagaaa taataatcat tatcacttaa aaatcataac ccttaaaatt
     4741 gtagctcgca atactttatt taaataattt tcatttttca tgtaaaattt gtgacattgc
     4801 aaaaatgttt tgtataatca cttccatgca aaaaacctcc gcttcaaatg tcattgatag
     4861 acatttaaag cggaggcatg tacatgttat tttatgattt atttcgtaga tttttttcta
     4921 acaattgatt gagcttttca atatttttat tcaactgttc tatattcgtt gcttgttttt
     4981 ttgaatttag ttgatgcttg ttttgataat ctattaaagc atcatataag aacaatgaac
     5041 ttgctgattg ctgtttcact tcttggtaca atgccatcgt tttatcatct agtactattg
     5101 aatgtgcatc atcttgccca tgtgcatctt gatgtttcga tacatttgtt gcaccttgtg
     5161 gatatacttt aaaggttgtg tcgtatgttt ccttactatc tttagcttca gattcctgtg
     5221 attcaaccgt tttatatttt tcaagtgcat gtccttcaat atcaactcgt ggaataatgc
     5281 gattcaacca tgctggtaaa taccacgaac cttttccaaa caatttcgtt aatgcaggaa
     5341 ttaacatcat tctgactacg aaggcatcaa agagtacacc aaacgctaat gccataccca
     5401 ttgatttaat catgacatct tcttggaata caaacgcaaa gaagacacta aacataatta
     5461 atgcagctgc tacaataaca ggaccgcttt ctttcaatcc tactttgata gaataatcat
     5521 tatcccctgt tttactatat tcttcatgaa ttcgcgacat aaggaagact tcataatcca
     5581 tcgctaatcc aaataagata cctatagtaa taaccggtaa aaatgctagc attggtcctg
     5641 tcgtttcaat accaaacaga cctttcataa aaccatcttg cattactaat gttgtaaatc
     5701 ctaatgttgc cattaatgac aagacgaatc ctaaaactgc ttttaatggt attagaattg
     5761 aacggaagac aatcattaat aagaaaaatg ctaatacaac aatgactgag gcaaataaag
     5821 gtatcgcctc atttaacttt ttagacatat caatattaat gacactttgt cccgaaatct
     5881 ccgttttgaa cccatattta tcttgtgcat ctttatgata atctcgtaaa tcatgcacta
     5941 aatcatttgt actctctgca ttaggccctt gcttaggtat cacgaccatc aaagcgtaat
     6001 cattatcttt actcatttgt ggtggcgtaa cgatatctac atttttctta tctttaatat
     6061 ctttatatac agactgtaaa tcttgttgta atccttgtgg atcatccttt ttatctttca
     6121 catttatcaa catcggtatt tggccattaa atccttcacc aaatttatcc gagataatat
     6181 cgtaagcttt tttctgtgta gaatctgctg gtttaacacc gtcatctgga ataccaagtc
     6241 gcatatgact aactggtatt gcagctgcta ctaatatgat taaacctagt aatactgccg
     6301 caagtgcatt tcctgtaata aatttagacc atggcgtatc aatatctttt ttgaatttag
     6361 actgtaattt attcacttta atgcgtttat ggaaaatgct tattaatgca ggtaataaag
     6421 ttaaagcgct aagtactgca aaaacaacac taattgccga agcaaatccc attaccgcta
     6481 agaagtcaat gcctactaat gataaaccac atactgcaat tacaactgtt acaccagcaa
     6541 aaacaactgc actacctgct gttcctattg caagaccaat gcctttaatg taatctgttt
     6601 cagttttcat aacttgtcga tatctgaata aaataaataa tgcataatcg ataccaactg
     6661 ctagtccaat cattacggct aatgtcagtg tgacatttgg tatatcgaat gcataagtta
     6721 acaaactgat aatacctaca ccagaggcta gaccaatcaa tgcacttata attggtaatc
     6781 ctgcagcaat gactgaaccg aatgtgatta acagtacaac aaatgcaaca ataataccaa
     6841 ctagttcaga attaccgcct acttctgtag atgtcattcc tgtaccagtt aactctgttt
     6901 gtacattatg attgtcacgt aagtctttca aatgactttt aacattatct ctagagccat
     6961 cttttaaaga tgtttgacta acgtcatatg tgatatctgc aaatgcagtt gttttatctt
     7021 tactaatttg cttattttca taaggatctg atattttatc aatgtgcttg tcatcttttt
     7081 taatatcatc taacgttttc ttaatatctt tagtaatgtt cggttgcaca ataccatcat
     7141 ctttagtcgt cttaaagaca acacgtattt gtgccttttc actatcttga ttaaaatgtt
     7201 tttcaatctt tttattcgta tctaacgact ctaatcctgt cattttaata tcattgtcaa
     7261 atttcggtgc atttgtagca agtggtatca atattgcagc tacaatcact atccatgcaa
     7321 tgaccgcgga ccatttatgt tttgcgatga atgtccccat cttatataaa aattttgcca
     7381 aagtatattg cctcctttta aaatcaacgt tatagtttaa atatacagtg tagattattg
     7441 ttcgattata gtatctatcc ccgacctctt aaagaatcaa ttggaaaatt ttgtatatta
     7501 aactacacac aaaggagaaa tgtagatgaa agagactgat ttacgagtta taaagacaaa
     7561 aaaagcattg tcgagtagct tgctacaatt gttagaacag caattattcc aaacgattac
     7621 tgtcaatcaa atttgcgaca acgcactcgt acaccgtaca acattttata aacattttta
     7681 tgataaatat gatcttctag agtacttgtt caatcaattg actaaagact actttgctag
     7741 agatatcagt gaccgtctta atcatccatt ccaaacgatg agtgatacga ttaataataa
     7801 agaggatttg agagaaatcg cagaattcca agaagaagac gctgaattta ataaagtatt
     7861 aaaaaatgtc tgcattaaaa ttatgcataa cgatatcaaa aataatagag accgtatcga
     7921 tattgacagc gacatcccag ataatctcat attttatatt tatgactcgt tgattgaagg
     7981 ttttatacat tggataaaag atgaaaaaat tgattggcct ggcgaagata ttgataacat
     8041 tttccataga ttaatcaata ttaagattaa atagtagatg agaaactcat gagcgttacc
     8101 aacattcata ataaaaacga tagtgtacac gttaatgaat tcgtgtacta ctatcgtttt
     8161 ttatttttat cgtgcttatc gctattaaaa caactgatac acaacacata aactatgaag
     8221 aaaaaaataa atccgctatc taaatgactt tgactcagtt gtttaaatga ccaaattgct
     8281 aatacaattc ccattattat tgaaataacg tatctcacat tcttatacct ataatccttt
     8341 tctaaaaata tggttgctat tacttaattt ttaaagttat aaataaaaag agccaaccgc
     8401 aatggatggc ccttgttcat tatgaagcat tagaacattt ctgaaacaac cttttgttct
     8461 aagaagtgta ataagtagtc tggactacct gttttagcgt ccgtacctga cattttgaaa
     8521 ccaccaaatg gatggtatcc aacaactgct gaagtacagc ctctgttaag gtataaattg
     8581 cctacatcaa attcgtttac cgctttaatc caatgctcgc gattatttgt aatcactgca
     8641 ccagttaaac cgtaatctgt atcatttgca acctcaattg cttcatcaaa atcgttaact
     8701 ttcacaaagc caacaactgg accaaaaatt tcttcttgca tgattctatc tttagattta
     8761 agtcctgaaa tgattgttgg ttctacaaag taaccttttg aatcatcagt gccgccacct
     8821 tgttctaatt taccttcttc tttaccaatc tcaatataat ttttaatctt atcaaattgt
     8881 tttttattaa taactgggcc catatacgta ttgtctacag tattgcccaa cgttaattct
     8941 tttgttaatt tgattgattt ctctaatact tcgtcataaa cgtctttatg cacaattgca
     9001 cgtgaacatg ctgaacattt ttgaccagaa aaaccaaatg ctgacgttac aatagcttct
     9061 gctgccatat ctgtatcaat attttcatca actacaatgg catctttacc acccatttca
     9121 gcgataacac gtttcaagaa gttttgacct tcttgaacaa cggcactacg ttcataaatt
     9181 ctagtacctg tcgcacgtga tcctgtaaat gtaacgaaat gcgtatcttt atgatcaact
     9241 aagtaatcac caatttcttt cggatcacca ggaacaaagt taactacgcc ttttggtaat
     9301 cctgcttctt ctaaaatttc cattaattta taagcgatat aaggtgtatc ctcagcaggt
     9361 ttcaataaca ctgtattacc tgccacaact ggtgctaaag ttgtaccagc cataatcgca
     9421 aacgggaagt tccacggcgg aattgtaaca cctgtaccaa ttgatttata gaaatattta
     9481 ttgtgttcac cttcacgatc aagtactggc ttaccttgag ccaagtccat cattgaacgt
     9541 gcatagtatt caataaaatc aataccttca gctgcatcac caactgcttc atcccatggc
     9601 ttacctgctt cataaaccat aattgctgca atttccgctt ttcgacgacg aataattgcc
     9661 gaaacacgta acataagctc tgcacgatca tttgctgacc atgttttcca agatttataa
     9721 gcttcgtttg ctgctttaaa cgcatcttca acatcttgtt ttgttgcctt tgatgcattt
     9781 gcaatcactt gtgatgtgtc tgcaggattg attgatttaa ttttgtcatc tttgaaaatc
     9841 ttctctccat taatcactaa tggtatgtct tgacctaatt ctttttccac gtctttcaat
     9901 gctttcttaa acatatccac attttcttgg actgaaaaat cgtaaccagg ttcattttta
     9961 aattctacta ccatgtacac ttacccccta taaattttga aagtggttta accctttgat
    10021 ttaatgatat aacatcattt aaactcattt tactatgatt aaggttagtt ttgcaatcgc
    10081 tttcattttt atgttttatc acttattctc aagtattttg aaattgattg gttacttttt
    10141 aaaatttata tgggtcgcaa ctgctacttt atcgtttcgt catttaatgt ttcggatggt
    10201 aggtcattat caattttacg aacgacttta caagggtttc caaccgctaa gctgtgtggc
    10261 ggaatatctt tagtgacaac actaccagca ccaatcacac tgccttctcc aatcgtcacc
    10321 cctggtaaca cggctacatg accgccaaac caagtattac tgccaatatg aatgggtccg
    10381 gctttttcaa aaccttcatt tctatgatgg aaattaagtg gatgtgtcgc tgtgtagaat
    10441 ccacaattag gtcctataaa aacattatcg ccaattgtaa tctgtccacc atccataaag
    10501 taacaattgg tgtttacata gacatttttt cctagtttaa cgttccaacc ataatctgta
    10561 tcaaaaggaa tcgaaatact tacattgtct gttgttgttt gaaataattg atcaattaat
    10621 tcctttcttt tatttgtagc actcggtctt gtatgattta attcaaagca aatatctttc
    10681 gctcgtgcac gttcattgat taagtattga tcaaagtttg catcgtacca tttttctgct
    10741 aacatttttt ctttttcagt cattacacct ttcaactcct aataacttat ttacttgttt
    10801 aaaagttaat caaataaacc ttcgcctatg caactaatac gctataacat tatgaaatca
    10861 tgaccttatc acccttatct atacaattct cgcatcaaat actgctaaag tagtagataa
    10921 attcaatact acagacgcat tcatttttta atctattaac gtacaatgtg agtaagagaa
    10981 atataaagga gtatgatagc gatgagaata ttaattacag gcacagttgc tatcttaatc
    11041 attctaggtt tggtcaaaac gatacaagat tacgaaatga caaacgacac gagtcgtcag
    11101 ttgtcagaca acaaagatga tgataaagtc atccatctta ataattttaa aaatttacat
    11161 gcgaaagaat ttaacccatc tgatttcttt taagtcacct aagaattgca aatccagaag
    11221 tcatttaagt tttacctttc attcatacat cctttaatat taattacgac ttcttttata
    11281 tagatgctaa gtagagagat tgttgtgcaa tgtttgcacg gcaatctctc tttttctttt
    11341 taaaattggt aaaagtaaaa cgcaacgatt gacttatata cctatagggg gtacattaga
    11401 cgtgtaacaa tgaatcacag ggaggcaata atgtggctaa tacgaaaaaa acaacattag
    11461 atatcactgg tatgacttgt gccgcatgtt caaatcgtat cgaaaagaaa ctgaataaac
    11521 ttgatgacgt taatgcccaa gtgaatttaa ctacagagaa agcaactgtt gagtataacc
    11581 ctgatcaaca tgatgtccaa gaatttatta atacgattca acatttaggt tacggtgtcg
    11641 ctgtagaaac tgtcgaatta gacattacag gtatgacttg tgctgcatgc tcaagccgta
    11701 ttgaaaaagt gttaaataaa atggacggcg ttcaaaatgc aacggtcaat ttaacaacag
    11761 agcaagctaa agttgactat tatcctgaag aaacagatgc tgataaactt gtcactcgca
    11821 ttcaaaaatt aggttatgac gcgtctatta aagataacaa taaagatcaa acgtcacgca
    11881 aagctgaagc gctacaacat aaattgatta agcttatcat atcagcagta ttatctttac
    11941 cactattaat gttaatgttt gtacatcttt tcaatatgca tataccagca ctatttacga
    12001 atccatggtt ccaatttatt ttagctacac ctgtacaatt tattattgga tggcaatttt
    12061 atgtaggtgc ttataaaaac ttaagaaatg gtggcgccaa tatggatgta cttgttgctg
    12121 ttggtacaag tgcagcatat ttttacagta tttatgaaat ggttcgttgg ctaaatggct
    12181 caacaacgca accgcattta tactttgaaa caagcgccgt actaattacc ttaatcttat
    12241 tcggtaagta tttagaagct agagcgaagt ctcaaacaac caatgcgctt ggcgaattat
    12301 taagtttaca agctaaagaa gcacgcattt taaaagatgg taatgaagtg atgattcctc
    12361 taaatgaagt acatgttgga gatacactta tcgttaaacc aggtgaaaag atacctgttg
    12421 atggcaaaat tattaaaggt atgactgcca tcgacgaatc tatgttaaca ggtgaatcta
    12481 tccctgttga gaagaatgtt gatgatactg taattggttc aacgatgaac aaaaacggta
    12541 ctattactat gacagcaaca aaagttggcg gggacactgc gttggcaaat attattaaag
    12601 ttgtcgaaga agctcaaagt tctaaagcgc cgattcaacg attggcagat attatttctg
    12661 gttatttcgt tcctatcgtt gttggtatcg cactattaac atttatcgtg tggattactt
    12721 tagttacacc aggtacattt gaacctgcac ttgttgcgag tatttccgtt ctcgtcattg
    12781 cttgtccatg cgcattggga cttgctacac caacttctat tatggtaggt actggtcgcg
    12841 ctgctgaaaa tggtatttta tttaaaggtg gcgagtttgt tgaacgcaca catcaaattg
    12901 ataccatcgt tttagataag acgggtacca ttacaaatgg tcgtccagtc gtgacagatt
    12961 atcatggtga caatcaaacg ctacaactac ttgctactgc tgaaaaagat tctgaacacc
    13021 cattggcaga agccattgtc aattatgcaa aagaaaagca attaatatta actgagacaa
    13081 caacatttaa agcagtacct ggccatggta ttgaagcaac gattgatcat cactatatat
    13141 tggttggtaa ccgtaaatta atggctgaca atgatattag cttgcctaag catatttctg
    13201 atgatttaac acattatgaa cgagatggta aaactgctat gctcattgct gttaattatt
    13261 cattaactgg tatcatcgca gtggcagata ctgtcaaaga tcatgccaaa gatgctataa
    13321 aacaattgca tgatatgggc attgaagttg ccatgttaac tggcgataat aaaaacactg
    13381 ctcaagccat tgcaaaacaa gtaggcatag atactgttat tgcagatatt ttaccagaag
    13441 aaaaagctgc acaaattgcg aaactacagc aacaaggtaa gaaggttgcg atggttggtg
    13501 acggtgtaaa tgatgcacct gcattagtta aagctgatat cggtatcgcc attggtacag
    13561 gtacagaagt tgccattgaa gcagctgata ttactattct tggtggcgac ttgatgctta
    13621 ttcctaaagc catttatgca agtaaagcaa ccattcgtaa tattcgtcaa aatctatttt
    13681 gggcattcgg ctataatatt gccggtatcc ctatagctgc attgggctta cttgcgccat
    13741 gggttgctgg tgctgcaatg gcactaagtt cagtaagtgt tgtcacaaac gcacttagat
    13801 tgaaaaagat gcgattagaa ccacgccgta aagatgccta gattccttaa taatgaagga
    13861 ttcgttggtg attctgagat aggctagtga ttggctctat aatgtc
//
LOCUS       DAKTGS010000074        13739 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.074, whole genome shotgun sequence.
ACCESSION   DAKTGS010000074 DAKTGS010000000
VERSION     DAKTGS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.074"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            196..516
                     /locus_tag="O0286_001726"
     CDS             196..516
                     /locus_tag="O0286_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCY0170105.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            530..832
                     /locus_tag="O0286_001727"
     CDS             530..832
                     /locus_tag="O0286_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170106.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            1007..2098
                     /locus_tag="O0286_001728"
     CDS             1007..2098
                     /locus_tag="O0286_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCY0170107.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            2159..3214
                     /locus_tag="O0286_001729"
     CDS             2159..3214
                     /locus_tag="O0286_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0170108.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            3219..3479
                     /locus_tag="O0286_001730"
     CDS             3219..3479
                     /locus_tag="O0286_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170109.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            3491..3874
                     /locus_tag="O0286_001731"
     CDS             3491..3874
                     /locus_tag="O0286_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0170110.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            3909..6404
                     /locus_tag="O0286_001732"
     CDS             3909..6404
                     /locus_tag="O0286_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0170111.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            6458..7816
                     /locus_tag="O0286_001733"
     CDS             6458..7816
                     /locus_tag="O0286_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCY0170112.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            7821..9668
                     /locus_tag="O0286_001734"
     CDS             7821..9668
                     /locus_tag="O0286_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170113.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            9658..10704
                     /locus_tag="O0286_001735"
     CDS             9658..10704
                     /locus_tag="O0286_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0170114.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            10711..11301
                     /locus_tag="O0286_001736"
     CDS             10711..11301
                     /locus_tag="O0286_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170115.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            11357..11713
                     /locus_tag="O0286_001737"
     CDS             11357..11713
                     /locus_tag="O0286_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCY0170116.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            11822..12325
                     /locus_tag="O0286_001738"
     CDS             11822..12325
                     /locus_tag="O0286_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0170117.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            12306..12842
                     /locus_tag="O0286_001739"
     CDS             12306..12842
                     /locus_tag="O0286_001739"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCY0170118.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            13062..13610
                     /locus_tag="O0286_001740"
                     /pseudo
     CDS             13062..13610
                     /locus_tag="O0286_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         5006 a         1552 c         2605 g         4576 t
ORIGIN      
        1 atttcaattg aagacttaga attttttgag gtgatgaacc gataggtttg attaaaaata
       61 tgataggtca ggagaagcat agacactcca ttatcaaaaa tgcagtggta cggatttgcg
      121 ggagttattc ctattcgtgc gaccctttag aagtcgttta attttattaa aaattttgta
      181 aaggatggat atattatgaa accagtattg gatattaaaa aaactttagg acaattaaac
      241 tttttaggtg tagatgaaaa atataaatat gaaaatggag agcgtactga taaaactgtt
      301 tatgcatata aattagcgag tcaagaacaa ggcgaacaaa tcactgttaa aacgcctaat
      361 aaagtggaat tagactattt acaagaatgc gaattagttg aaccagacgt taaaatgtat
      421 gttcaaatgt caggcgattt tggaactatc gcatatagct ggaatgcaga agatattaaa
      481 gtagttggtt ctaatagtgc acttaaacaa aaataattag gtgatgtaaa tggattatta
      541 tacagcagat agattatatc gctacacaaa tagttcaaat ttgagtgaac ctattttaaa
      601 ctatgttgct agtcgtataa attggggcga taaagtttca ttaatgactt tagctaaaga
      661 aatacagtct aaatttaatg atagttacgt taaagaaaat acggtaaaag gtcggcctaa
      721 aatttacgct gacctttgtc tattgtgtat gagcttgagt gaagcaggac atggcagaat
      781 gctacaagtg aatttggaag attgtattta tattggcgat attgatgttt aacatatagt
      841 attagtgatt taacatgacc gtgtagcttt tagcgtatgc aggcgcagaa atgcaagcac
      901 ggagtgcgtt aagcatttgg gcggagcctg cgacaaatac gcgttaaaaa agcgagaacg
      961 gtcatgtatc aaatcgcttg cgatttgata ggaggcataa ataaatatga ctttaaaaaa
     1021 ccagtgtacc ccccttacta ataggggggt agagcgtaca aataaaagtg ctgtggaggc
     1081 agttgttgac tgggttcagg tcactttcca tatagcccct atttcagcag taattgagga
     1141 cgttatcggt ttgcctataa cgttgttcaa aaaacgaaat agtggcattt acttttataa
     1201 tcgcggttat gaattttcaa acattaaatt gtattattca agtgatgatg aatcaatggg
     1261 cattcactta cagttaactg gtacaggttg tagagagttt gaacatcatt tacagcagtt
     1321 gaataaaact tggcaagatt tttttgacaa atgtttatca gttaatgcaa attttacaag
     1381 aattgatatt gcaattgatg attataaaac atatttaaaa gtgcctttat taattaaaaa
     1441 ggctgaaaaa gctgagtgtg tttcacgttt tagagctgga agcgcaataa acggttttaa
     1501 tttgtcagac ggaagaagta aaggcgctac tttttatatt ggttctaaac aaagcaattt
     1561 atattgtcgt ttttatgaaa agaattatga acaagcgttt aaacgtcatt gtgatgtcga
     1621 agacattggg ctatggaatc gttatgaaat acaaatgcga aaggcatatg cagttaattg
     1681 tgcgaaagtg ttgagtagaa cagataatat aagcgaaatt gtaaagtcta tattgcataa
     1741 taatttgcgt tttatctctc ctcctaaagg tggaaatgat aaaaatcgta agcggtggcc
     1801 attatataga ccttgggcgt tatttattaa agatactgaa aaattgaatt taactactag
     1861 accaacatta aagtccattg aggataatct tgattggtta tgtaaacaag ttgctactac
     1921 tttagataca gtgttaactg ccgaaagtat ggcgcaatct gaggggttgc ttacagatac
     1981 tgatttttta gataaaattt tagcgcattc acaatttaat gatgagcata cgaatagaat
     2041 taatcattat ttggaggctt taaaacaaaa aaagcactta tcaaaagata agtgttaaaa
     2101 aaataactgg gatgtgattt ttattaaaaa tttcgtaaag gattttagaa aatcgtttat
     2161 gaaaataata agcaagtaca ttttaacacg attttctaaa aacggaaagc attttgatat
     2221 tcctaaagaa caacgccgag taattccaaa atttaaaaat cgtcgtaaga tgattgtaat
     2281 ttgtttttac tcattattag ctattttgtt aattctttta ttagcttctt ttattaaagc
     2341 tacaagagca aataatgaca gtaaagaagc agttaataaa acgaatatga ttcaaaaaaa
     2401 gtatgaagat aatgcgaata cagttcagta tagtcctaaa ttaaagttat atgctgataa
     2461 gtttattgat acgtatatga atatctcgaa agattcaaaa gaattggaat ctagagaaaa
     2521 agaattgttg aaatattttc catcagatta taaaaaacct gatgaaaaaa tttcagatac
     2581 agaacgaaaa ttgaatagta aagaatttta taatattaaa cgtaaagata aacaaacaat
     2641 tatacagtat attgttaatt atgatgtgaa tattactgaa aagaaagaag ttaaagttaa
     2701 gaagaaaaag aaaagtgaaa aagataaaga tgagtatgaa acaaaaacag aagaaaagca
     2761 acgtaaagta aatcaaaaca ttttaataaa tatacctatt aagagtgaaa ataataagta
     2821 tgttgttgtt gaatatcctt attttacgcc aatccctgat agtcaattga atcaagctaa
     2881 aatggttgag gataatttga aagacaataa acgggaagac aatccgaaag caaaagcttt
     2941 tattgaagat ttctttaata aatatgcttc tagtaagtct gatgatatgg cctatttaat
     3001 ggataaccct gagggcttag aaggaactag agaagtttct cagataagag aaattaggtt
     3061 atatccaaaa ggcgatgatt atgttgctaa agttgaaatt ttgatgaaag ataaagattc
     3121 tcctttggag aatttggagc attacacatt agatataaca aaaaaagatg gtaaatatta
     3181 cgttaaaaat atgactaatt ctattggagg ttaatgttat gagtggttta tttaattttt
     3241 ttgttttagg tgctgaccgt ccatcacttg gtggagttgg tgactggata agtaatgaag
     3301 taggtacggg tattggttta gtagtattgg ttgttggtat tgtaaaatgg gctagtggta
     3361 aatatgggca tatggttata ttatttgttg ttggtggctt tttattctta gtttctaaag
     3421 gtcctgaaca ggtatttaat gcgattgcag gtgtttggaa aatgattttt ggcggttgag
     3481 gtgattccat ttggataaaa aagcttataa tttaaagcga acttttgaac aaccgatagt
     3541 tatgtatgaa tttacagata agtttcgtat taataagggt tttcgtttag atttttgggc
     3601 tacatttttg attgtgtggt ttatattatt tttgttattt tggtatttat tacagcctgt
     3661 aattatgtcg attggtggac ttatgtttat ttactttacg gttgcacctt attatataac
     3721 gaaatatatt gttaagttaa aacaagatgg aaagaaatta tttttcttct tatgggattt
     3781 tgtaatattt gtttttaatg tgcagttaag aaaagttaaa ttaagttatg atgaagaagt
     3841 agaatatcat gataaaaaaa taacatttaa atagccccta ttggggttat ttttattgga
     3901 gggatgacat gaatttaaaa gcaccgtatg caggtttaag agataatttg ttattaacga
     3961 aaactggcga agtttgggcg tattatcgtg ttcgttctga gtctatatca acagcaaatt
     4021 atgatgcaaa agaagaatca aaaaagcgta tgcgttattt tttagagtcg attaaaggtt
     4081 atcaagattt tcattttgaa atgtatgacc acgatttaga tttgcgaaat aaatttaaag
     4141 aaattagtaa agattttgat gatgaagctt ttaatgtagc tgaatattat tcaaaagaaa
     4201 ctattgacgt attagaacaa gaattacaaa tgattacgca taattctttt gttatgggtg
     4261 ttaaaatacg tgaattagct gacgatgcag ttacacttaa agagatttta aaaagtacat
     4321 tatcagatac ggctgagcgt attgtttcga attttggttt tgatgtgaat ttagacgata
     4381 aaattttaga taagtataaa acatttgaac gagatttggc tgatagtttt ctaggtattg
     4441 gaggcgagcg tttaactgag aatgaattgg cttatattat tagaaagcct tttatttcaa
     4501 atgcaacaca tgacgtacag gaagaatctt caagaagagc tattacagat atttataatg
     4561 cagtaattga ccctactcgc ttgagtgttt tagaagttga aaatgataac gagaaatttt
     4621 atacaacaac agttgttgtt gatgattttc cgttagatat ggaatataca catttatttg
     4681 agaaagctca aaatatgcct tttccggttg aatgccatgt aaaagcaaaa atcattaata
     4741 atgagaaagt taagaaaaaa tttgaccgtg cgaaaataaa tcataaacaa caagcaaaag
     4801 aaaagcaaga tactggcgac agtgcttcgg aagatgttca agataatttg tttgtattga
     4861 atcaaatgga acgagaagtg actggctcag gtgtatttat tgagtgggta tttatgtttg
     4921 taattaaaag tgatgattat agagattgta aacgtaaagc aaagcgtttg atgaaacgat
     4981 taaaggaaac tcaaatatat gcagttaatc cattagctga ccagttacaa ttgttttatc
     5041 aatgtttaca tggtaatgat ttatttatta ataagtattg gttacaaaga acaacggcaa
     5101 caggtatagc agagaattta tttggtgtgt ctcaaagttt gggaacaaaa acgggttttt
     5161 atatagggcg tattgataaa tttatacgtt ctgtttcacg tgaggaagct gttgcaagtt
     5221 caagggatat aattttgttt agtttattgt tggccgcaaa aggtattaag ggggctgtca
     5281 gtgatagtcc gcatgtttta attactggtc aaactgggaa aggtaaatca tttttagcaa
     5341 aattattatg gatttataca tcatttttta aaggtcaaat gttgtactgg gatccaaaat
     5401 ctgagtttgc agaatggttt gacagagtaa cagaaagtaa ggaaatgcaa gaaaagtatc
     5461 ccctatttat taatcattta aaaacattta aatatgtaac attgaattat gaagattcaa
     5521 ccaattatgg ttgtctagac cctattgtat ttttagatgg tattgaagct aatgagatgt
     5581 tgaaatcagt gtttgatgaa attaaatctt ttgaaaatta tcatcatatt gaaacagcaa
     5641 tttatcaagc tatttctgat acggttgaag aacgtgaaaa tggtaaaaaa gttggttcat
     5701 taaatgtaat tgaaaaatta cagaataatg aagatgagca tgtaaaaaat gcaggcgatt
     5761 tattatttga aaaaacgcaa aacaatattt taaaacttgt ttttagtgat ggtacgaatc
     5821 cagctttaaa tattcaagaa aaagcaacta ttttacaggt taaaggttta gatatgccta
     5881 aagcagatga tgatacatca agttattcaa caagtgagaa aaatgggatt acattaatgt
     5941 tattaattgg taagttttta gagaaattcg gttctcgtag agatgttcaa acgactatat
     6001 ttattgatga gggttgggct tttagtgctt caagacaagg taaaaaagtt ggtaagcgta
     6061 taaaaagaac gggacgttct gagaataatt cacttgtatt tattacacaa tcggtaaaag
     6121 ataaagcaga tgatgatggt ggtaactttg gttgtcattt tgcatttgat gaaaaagatg
     6181 aacgtgagga tattttaaaa tctttaggct tagaatattc aaaagaatct cctgaaaaca
     6241 tggagatgtt gaaagatttg aagaaaggtc aatgtatatt cagtgatttt tatggacgtg
     6301 ttggaaaaat ggttgtacat tgtccatttg aggaaatgac tgaagcattt agaacacaag
     6361 aagattctgc aagctctaaa gctgaagaaa aatttgcgat gtaattttta gcacatacaa
     6421 aattgtatgt gctttttatt ttaaaggagt ggatttgatg gcaatgaatg aaattgcttt
     6481 atttaaaggc gtacgtatac agccttatca gaagtatatt gattttatgt ttgcaagtgt
     6541 attagcattt atttttttgg tatatcgtat atatattttg tttattgatt ataaatcagt
     6601 taataaaaaa ttggatatat tgacgttgtt tgcatatttt aaaccgcatt tattgtattt
     6661 attaattggt acagcaatta tatttgtgat atgtaagctt attgctcagt ttttaatacg
     6721 ttttaaaaca aaagatttgg ctgaaatgat tgaaacacaa ggttttcata atcgtacagt
     6781 aatcaagaaa acaactgagt atatccctat tgattataaa caaactgaat atgattatta
     6841 tccaacaatg ttttataagc gtggtaaaaa atcttttgtt attcatatta agaaagacgg
     6901 ttcgaggttt caagataatt atttagagtt agagacgatt attgaaccaa tgtttaattc
     6961 tgaattagta gaaaaaaagc atattggtcg ttatttgaga tatgaattta tgccacttaa
     7021 atataaaaag cgtattgtta tgaatggcga acaagaatca aatacaattt tttatgatac
     7081 aaaaattgct attacgcatc aaattacttg ggattttgtg aaagcacctc atggattggt
     7141 tacaggtata accggaggtg gtaaaacata ttttttattt tatgtgattc gtgagttatt
     7201 ccgtagacat tcagaagtac gtttattaga cccaaaagtt tcagatttga gctttatgaa
     7261 gcgcgttatt ggtgatgata aagttgcaga tactaaaggt caaatattaa aacaattgag
     7321 agaagctaat aatgaaatgg aagagcgttt tagattaatg aatgatagtt cggattataa
     7381 aatcgggaat gatttccgta attttgatat gcgcccctat ttcattattt ttgatgaggt
     7441 aacagctttt acttcaactt tagataaaaa agaattacaa gaaatgaatg attatttaat
     7501 taatattatt atgaaaggtc gtcaagctgg tgtatttatg tttttaacag cacaaagacc
     7561 agatgcagat gtgattaaag gtaatgtgcg tgaccaatta gggttacgtg tgtctttagg
     7621 taatttgtca aatgatggtt atcgtatgac ctttggtcaa actgataaag aatttcaaac
     7681 gattcatgat agtgatatcg gccgaggtta tatttctata cttggacaat ataatgaacc
     7741 aattttattt gatgcacctt taatggaaca atatgatttt gtggaagatg tgaaacaaat
     7801 attaaataag gagtgatttg atgaagcata aaagtgtgta tcttttatgc tttttaattt
     7861 tgcctattct gcttttaaca tctgtgagtg cttatgctgt ttctaatcca gttggagagc
     7921 cctcacatgc agttaagcca aaaattgaaa agtatgattt atcacattat cgttcaatat
     7981 atagtgaaaa aggcgactgg aatccgtttg gtcaagaaga aatttcacgt caaattaata
     8041 atgtgtcaga ttttttcttt tctatgacta agattttagc aggtgttaca gattatgcga
     8101 ttgaaaattt atttcaatta gatgttatta atgattttgc ggataaaatc ggcgattttg
     8161 ttggcgatat atatcaaaag ttattgagta atttagcatt aacattattt attattgttt
     8221 gttttaacgc atttattatt tttagtgtac aaggtaatgc aagagaagca ttaaaacgag
     8281 cgtttttaat tatgtgttta attggttttg gtgttggtat acttgcgaat gcaggtaata
     8341 ttatacgtgg tacaaacaat attggtaagg acttaaataa tataattatg aatagtacat
     8401 cttctattga tggtaatatt gattatattc atgagaattc aggtatgaat catatacgta
     8461 atcaattatt tgatatgacg atatatagaa catatttagt aatgaattat ggaactgtcg
     8521 acgaaaaaga gataaaagca aaaggtaaag accgtattga taacatttta aaacttgatt
     8581 atgataaaaa atcagaaaaa gaattagatg atattgttaa agatgaggtt gaaaagaaaa
     8641 ataataaata tatgacgcaa ggttatgtat ttcaaaagtt ggctatttca gtgattggtt
     8701 ttattattac actttttatg tcgatagtat ttttatcaat aagttttgcg aagttgattt
     8761 tttctacatt tgctttattt ttattcttat ttttagtatt cagttggata gtgagtttta
     8821 taccaggatt tgaattatct gttttctctg cgtttgcaaa aacgttaggc tatattattt
     8881 taagtgcttg tatgacattc ttatttgtga tagttggttt gtgtatcaag cttgcaaata
     8941 gttttattga accggatagt caaaatgctt attttttaaa ttcaatattt ataattgtaa
     9001 tattgtttgt aatgtataaa aaacgggctc aaattatcaa ttttgtaagt cgtggtaata
     9061 ttagtttttc tcctagtgca attggtgctg gtgtgatgaa tcgaacacaa gaacgtttta
     9121 ataagattcg tcatgaacaa gcgcaaaata agaaagctaa acgagaaaat caaagagatg
     9181 aacccgcccc accacttcaa aatgataatg atttaagaag aagacaacag gataaaccaa
     9241 tgcctttatt tataaataaa gataatcaaa aaaatggtaa taagcgcaga gaacaacaag
     9301 agtcaatgaa tggtaacgat gtaaaatctg cttctgttga atcaaatgca aataattatt
     9361 ctaaacaacc acaaaaagca tcacagcaag aacatcaagt acgagaaaca agacagcgaa
     9421 aagatataca aaggtcgcca caagtggtta atcagccatt aaataatgag aatcattcaa
     9481 taaatcgtaa agaacagaag tcagttcaaa ctgcttatga tactgatgtt caaaaacgac
     9541 aaatacaaaa tgcgacacag aatcaacaaa gtagacaatc tggaaatcgt aatcaaccta
     9601 ttacgcgtaa ttctcagtca aaagaccgtt taaaagagca aaaggatatc aataagcatg
     9661 gtaaataaaa aagatattgc taaaaaagta ataggtaaaa cgccaatagg cgttaaattg
     9721 aaattagcga aagtcatcat cattttatgt gtggtggctt tttttatatt tccagtaatt
     9781 ataatgtttt tgttagcgcc tgatttaaat aaaggtaaag atgatgctgg ttgtactgtt
     9841 agtggcggta atgtttcggc aaatggtatt gataagttta acgaaaatgc taaaggtgga
     9901 aaattagagg gtaaaggaaa agaaatacag aaaattgcag aaaaaaataa agtacctgtg
     9961 aatattttta tggccattat tgcgagtgaa tcacaatggg gtaaaggaga aaatgcgaca
    10021 agacaaaata atcctttgtc tgttatgggt tcaaaatcga ttcatgattc aacatatcca
    10081 actattgaag atggtcttaa tgcgggagct aaaaatttgt atgatgttta tatttctaaa
    10141 ggtttagata ctcctaaaaa aataggtccc aaatatgcgc ctgttggtgc gtcaaatgac
    10201 cctaataata tgaatgcccg ttggatacca acagttgaaa aaattatgaa agatttaggc
    10261 ggttcagaag ctaaaacgtc atgtagtaat ggtaaaggaa agtctataaa gtttaatggt
    10321 aaattgccac attggtcaaa tgatgaccct ggaaaaggta atttatacac tgctggtcaa
    10381 tgtacttggt atgcatacgg tatgcgtcaa aaaatgggta aacctgtatc gacttattgg
    10441 catgatgcac ataaatggaa tgatagagct aaagctgagg gttataaagt tgataaaaat
    10501 ccggaacctg gtgcattatt tattgctgaa caaggtgcag gcggtcatga tgggcattat
    10561 ggtcatgtgg ctgttgttat tggtgtaagt gatggtggta aaacatttag aataacagaa
    10621 atgaattggg aaggtgcttt taaagttaat gaacgtacat tgaaaatgac tgacggatat
    10681 agttttattc atgataagga gtgaaaataa atgaagcaaa aaattattat gtttactctt
    10741 gtaacagtaa tattattttg tgctgtttta attggttatc agattcctaa acaacaagtt
    10801 aaaatgaaac aaaatcaaat tgaagatttg caggaagaac aacgtatatt aagagataaa
    10861 aatggagagt taaataaatt agtaaagaga caatcaaaaa cagttatttc tgatgaagaa
    10921 aaacaaatac gtgaagtttc ttcaaatttt gttaaacaga tgtttgaaat gaaaaaagat
    10981 tcttctttta aatcaaaagc accacaaata aagcctttag ttacaaagga ttattacgat
    11041 acattattta aggattctaa agataaatat gatttatatg atgatataac ggttaatgat
    11101 attcatgttt attttgatac ttatgaccct aaaaaagata gttataaagt ttttgttcag
    11161 ttcgacgaac gtattgaaac agatggagat gataaaattg aacatcgtca aacatctgca
    11221 caattggatt tagttcgtac agctgaaggt tggcgtattg ataatttaaa acgtttcaat
    11281 ttaaaacctc taggacgtta gtttgtttca agacgatagc aatgatataa tttacgtaaa
    11341 aaggagtgaa tttatcatga ggcgatggtt tgtattaatt ttaggattag ttatattatt
    11401 atctgcatgt ggtcagaaat atgataaaga aattgatgaa gttacaaagt tagaaaaaga
    11461 aagtattcaa gatgtgaaaa atacaaagaa gtataaaaat gttgagcggt caaaaagtta
    11521 ttataagatt tataatgatg gtgaggttat tataatgact tatatgcctt ttaaagatag
    11581 taatacaaaa gtttcaagag tatataaaat aaatcagact agcgataaat atgaagaaga
    11641 ttctaatatt gatgctgaaa agtttgagaa agataataaa ccagtttatg aagaaaataa
    11701 tatgaaaaaa taaagaatca actccctata cgctttagcg atggtatcgg gagttgatga
    11761 acatatatcc tcttataact tattttataa ataacctttt taaaagtaaa ggaggatata
    11821 catgaaaaaa agaaaaaaac gtaaagaatt agactataaa acacgtctag ctatcgcaaa
    11881 gtatttaaat gatggtttaa acatcattca gatttctaag attttaggtc gtgatgatgc
    11941 agttctttat agagaagtaa ataagcgttc tgttgatggt gtttataatc ctttattagc
    12001 tgaggaagat gctataaaat cgcgtagtat ttcagatgaa caagttgcta aacgtagacg
    12061 tttgaatgat gaaacaaaag catatattga agaaaaactt tcattaaaat ggtctcctag
    12121 acagatagca agtcaaattg aaaaagatac agggtattat ataagttatc ctactatata
    12181 tagatatatt cgcaatggat tggtaaaagt tgatgttaag cgtgatatga gacaagcagg
    12241 caagaaatat aataagtctt ctgaaaagcg tggcaaatta gacgtagggt ctcgtgttat
    12301 aaagtatcgg ctcaaaaagt attaataatc gtgaagaatt tggacattat gaaattgata
    12361 ctgtttggag tgttagacca tcaacatatt gcttgttaac tattattgaa agaaagacaa
    12421 gatatttata tgctagtaga ttaaatacac gtaaatccaa tgtagtttgt aatgagatta
    12481 taaatattat gaaaccatta ttacccaaaa gtataactat ggatagaggt aaggagtttg
    12541 ctctatttga agaaatagaa gatgaattga attgtagtat ttattttagt gatttgggtt
    12601 gtccatatca acgtggttct attgaaaatg caaatggact tttaagacag tattatccta
    12661 aaggtacgga ttttgcaaat ataacacaaa agtcattaga tgaagctgtt aaacaaatta
    12721 atgcacggcc tcgaatgata tttgactata aatcgagtga ggaaatgcta aagtaccatg
    12781 taagcacaca aaattgtgaa cctatattaa atgactgtgt gaggcacgaa cccgtcaatt
    12841 aatataggtt aatctaagcg aatggtttac aatgagcgcg gaaggacagg acgaataaat
    12901 tttgtatgta aaatgcataa tctaaaaaga agcatactat ttaaatctgt ggtttttgac
    12961 agatattact agtaaaatgc ttctttttct tcttctgtac tcttttagta atatttatta
    13021 aaaaaattta agaaattctc atttttctat tgacacactg ccatttaatt aaagcaattg
    13081 gagtattgtt actcattgtc ggcatcgtca taatgaatca atttaacaag aataatttat
    13141 tactaactga tcaaaagtat ttactgtttt ggcttctatt aggatttatt tttggtttct
    13201 ttccacctat tcaaacgaca attaatagtg ctttagctag tcatactcat tcaccagcct
    13261 ttgcatcatt agtatcattt acaattgggt caatagcgct attgatttta accgctattt
    13321 ttaatcgttc tttaaaacta aaaacaagtc atttaaaatt cggtaaatta aagcctatct
    13381 attttactgg cggtatactt ggcatggctt ttgtaacagc taacattatc ttaatgcctc
    13441 atatgggtgc agcattaaca acacttattg ggatgtttgg ccagattcta atgggcatat
    13501 tgatagatca ctttggatta tttggttcac ctaaaatagc aatgacatcc agaaaaacta
    13561 ttggtctatt atgtattttg acaggcatta tacttttaag attattttaa attaactttt
    13621 agcttatcat tttaacttgt aattattttt aaaagtgata agctattttt ttgtggtcta
    13681 aaaatcttta gaaaagcgtt gatttgggta gtatgctttg acacaacaaa ttttaattt
//
LOCUS       DAKTGS010000075        13643 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.075, whole genome shotgun sequence.
ACCESSION   DAKTGS010000075 DAKTGS010000000
VERSION     DAKTGS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.075"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            95..388
                     /gene="esxA"
                     /locus_tag="O0286_001741"
     CDS             95..388
                     /gene="esxA"
                     /locus_tag="O0286_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCY0170119.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            472..3501
                     /gene="esaA"
                     /locus_tag="O0286_001742"
     CDS             472..3501
                     /gene="esaA"
                     /locus_tag="O0286_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCY0170120.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3501..3959
                     /gene="essA"
                     /locus_tag="O0286_001743"
     CDS             3501..3959
                     /gene="essA"
                     /locus_tag="O0286_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCY0170121.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3931..4173
                     /gene="esaB"
                     /locus_tag="O0286_001744"
     CDS             3931..4173
                     /gene="esaB"
                     /locus_tag="O0286_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCY0170122.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4186..5520
                     /gene="essB"
                     /locus_tag="O0286_001745"
     CDS             4186..5520
                     /gene="essB"
                     /locus_tag="O0286_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCY0170123.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5542..9981
                     /gene="essC"
                     /locus_tag="O0286_001746"
     CDS             5542..9981
                     /gene="essC"
                     /locus_tag="O0286_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCY0170124.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            10011..10403
                     /gene="esaC"
                     /locus_tag="O0286_001747"
     CDS             10011..10403
                     /gene="esaC"
                     /locus_tag="O0286_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCY0170125.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            10419..10733
                     /gene="esxB"
                     /locus_tag="O0286_001748"
     CDS             10419..10733
                     /gene="esxB"
                     /locus_tag="O0286_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCY0170126.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10733..11407
                     /locus_tag="O0286_001749"
     CDS             10733..11407
                     /locus_tag="O0286_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCY0170127.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            11407..11724
                     /locus_tag="O0286_001750"
     CDS             11407..11724
                     /locus_tag="O0286_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170128.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11734..13578
                     /locus_tag="O0286_001751"
     CDS             11734..13578
                     /locus_tag="O0286_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCY0170129.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
BASE COUNT         5318 a         1904 c         2590 g         3831 t
ORIGIN      
        1 aatttatata ttatttgagt aaggagcact ttttcaaaaa atagtgtccc taaaaagttt
       61 tgataaactt aaaatattca ggaggtttct agttatggca atgattaaga tgagtccaga
      121 ggaaatcaga gcaaaatcgc aatcttacgg gcaaggttca gaccaaatcc gtcaaatttt
      181 atctgattta acacgtgcac aaggtgaaat tgcagcgaac tgggaaggtc aagctttcag
      241 ccgtttcgaa gagcaattcc aacaacttag tcctaaagta gaaaaatttg cacaattatt
      301 agaagaaatt aaacaacaat tgaatagcac tgctgatgcc gttcaagaac aagaccaaca
      361 actttctaat aatttcggtt tgcaataagc attctgaaat tggcaaagtc acattttcta
      421 atgtggcttt gcttatcatt tttttaagaa aacaactgaa aggaaataag catgaaaaag
      481 aaaaattgga tttatgcatt aattgtcact ttaattatta taattgccat agttagtatg
      541 atattttttg ttcaaacaaa atatggagat caatcagaaa aaggatccca aagtgtaagt
      601 aataaaaata ataaaataca tatcgcaatt gttaacgagg atcaaccaac gacatataac
      661 ggtaaaaaag ttgagctggg tcaagcattt attaaaaggt tagcaaatga gaaaaactat
      721 aaatttgaaa cagtaacaag aaacgttgct gagtctggtt tgaaaaatgg tggataccaa
      781 gtcatgattg ttatcccaga aaacttttca aaattggcaa tgcaattaga cgctaaaaca
      841 ccatcgaaaa tatcgctaca gtataaaaca gctgtaggac aaaaagaaga agtagctaaa
      901 aacacagaaa aagttgtaag taatgtactt aacgacttta acaaaaactt agtcgaaatt
      961 tatttaacaa gcatcattga taatttacat aatgcacaaa aaaatgttgg cgctattatg
     1021 acgcgtgaac atggtgtgaa tagtaaattc tcgaattact tattaaatcc aattaacgac
     1081 ttcccggaat tatttacaga tacgcttgta aattcaattt ctgcaaacaa agacattaca
     1141 aaatggttcc aaacatacaa taaatcatta ttgagtgcga attcagatac gttcagagtg
     1201 aacacagatt ataatgtttc gactttaatt gaaaaacaaa attcattatt tgacgagcac
     1261 aatacagcga tggataaaat gttacaagat tataaatcgc aaaaagatag cgtggaactt
     1321 gataactata tcaatgcatt aaaacagatg gacagccaaa ttgatcaaca atcaagtatg
     1381 caagatacag gtaaagaaga atataaacaa actgttaaag aaaacttaga taaattaaga
     1441 gaaatcattc aatcacaaga gtcaccattt tcaaaaggta tgattgaaga ctatcgtaag
     1501 caattaacag aatcactgca agatgagctt gcaaataaca aagacttaca agatgcgcta
     1561 aatagcatta aaatgaacaa tgctcaattc gctgaaaact tagagaaaca acttcatgat
     1621 gatattgtca aagaacctga tacagataca acatttatct ataacatgtc taaacaagac
     1681 tttatagctg caggtttaaa tgaggatgaa gctaataaat acgaagcaat tgtcaaagaa
     1741 gcaaaacgtt ataaaaatga atataatttg aaaaaaccgt tagcagaaca cattaattta
     1801 acagattacg ataaccaagt tgcgcaagac acaagtagtt tgattaatga tggtgtcaaa
     1861 gtgcaacgta ctgaaacgat taaaagtaat gatattaatc aattaactgt tgcaacagat
     1921 cctcatttta attttgaagg cgacattaaa attaatggta aaaaatatga cattaaggat
     1981 caaagtgttc aactcgatac atctaacaag gaatataaag ttgaagtcaa tggcgttgct
     2041 aaattgaaaa aggatgctga gaaagatttc ttaaaagata aaacaatgca tttacaattg
     2101 ttatttggac aagcaaatcg tcaagatgaa ccaaatgata agaaagcaac gagtgttgtg
     2161 gatgtaacat tgaatcataa ccttgatggt cgcttatcga aagatgcatt aagccagcaa
     2221 ttgagtgcat tatctaggtt tgatgcgcat tataaaatgt acacagatac aaaaggcaga
     2281 gaagataaac cattcgacaa caaacgttta attgatatga tggttgacca agttatcaat
     2341 gacatggaaa gtttcaaaga cgataaagta gctgtgttac atcaaattga ttcaatggaa
     2401 gaaaactcag acaaactgat tgatgacatt ttaaataaca aaaagaatac aacaaaaaat
     2461 aaagaagata tttccaagct gattgatcag ttagaaaacg ttaaaaagac ttttgctgaa
     2521 gagccacaag aaccaaaaat tgataaaggc aaaaatgatg aatttaatac gatgtcttca
     2581 aatttagata aagaaattag tagaatttct gaaaagagta cgcaattgct atcagataca
     2641 caagaatcaa aatcaattgc agattctgtt agtggccaat taaatcaagt cgacaataat
     2701 gtgaataagc tacatgcgac aggtcgagca ttaggcgtaa gagctaacga tttgaatcgt
     2761 caaatggcta aaaacgataa agataatgag ttgttcgcta aagaatttaa aaaagtatta
     2821 caaaattcta aagatggcga cagacaaaac caagcattaa aagcatttat gagtaatccg
     2881 gttcaaaaga aaaacttaga aaatgtttta gctaataatg gtaatacaga cgtgatttca
     2941 ccgacattat tcgtattatt gatgtattta ctatcaatga ttacagcata tattttctat
     3001 agttatgaac gtgccaaagg acaaatgaat ttcattaaag atgattatag tagtaaaaac
     3061 catctttgga ataatgtcat tacgtcaggt gttattggta caactggttt ggtagaaggg
     3121 ttaattgtcg gtttaattgc aatgaataag ttccatgtat tagctggcta tagagcgaaa
     3181 ttcatcttaa tggtgatttt aactatgatg gtcttcgtac ttattaatac gtatttacta
     3241 agacaggtaa aatctatcgg tatgttctta atgattgctg cattgggtct atactttgta
     3301 gctatgaata atttgaaagc agctggacaa ggtgtgacta ataaaatttc accattgtct
     3361 tatatcgata acatgttctt caattattta aatgcagagc atcctatagg cttggtgcta
     3421 gtaatattaa cagtacttgt gattattggc tttgtactga acatgtttat aaaacacttt
     3481 aagaaagaga gattaatcta atgttgatga atagcgtgat tgctttaact tttttaacag
     3541 catctagcaa taatggcgga cttaatattg atgtgcaaca agaagaggaa aagcgaatca
     3601 ataatgattt aaatcaatat gatacaacgc tatttaataa agacagcaaa gcggttaatg
     3661 atgcgattgc taagcagaaa aaagaacgac aacaacaaat aaaaaatgat atgtttcaaa
     3721 atcaagcgag tcactcgact cgcttgaatg aaactaaaaa agtgttattt tccaaatcta
     3781 acttagaaaa gacttcggag agtgataaaa gcccctatat tcaaaacaag caggagaaaa
     3841 aaatattccc gtacattttg atgtctgtag gggctttttt gactttagga tttgtcattt
     3901 tttcaattca taaagggaga cgaacgaaaa atgaatcagc acgtaaaagt aacatttgat
     3961 tttactaatt ataattacgg cacatatgac ttagcagtac cagcatattt accgataaaa
     4021 aacttaatag ctttagtatt ggatagtttg gacatttcaa tatttgatgt caatacacaa
     4081 attaaagtga tgacgaaagg tcaattactt gttgaaaatg atcgactcat tgattatcaa
     4141 atcgctgatg gagatatttt gaagttacta taggaggaaa aatagatggt taaaaatcat
     4201 aaccctaaaa atgaaatgca agatatgtta acgcctttag atgctgaaga agcagctaaa
     4261 acaaaattac gcttagatat gagagagatt cctaagtctt caattaaacc agaacatttt
     4321 catttaatgt acttattaga acaacattct ccatatttta tagatgctga attaactgaa
     4381 ctacgtgaca gtttccaaat acattatgac attaatgaca atcatacacc ttttgataat
     4441 attaaatcat ttactaaaaa tgaaaaatta cgttacttac tcaatatcaa aaatttagaa
     4501 gaagtaaatc gtacacgcta cacatttgtg ttggcaccag atgaattatt tttcacaaga
     4561 gatggattac ccattgctaa aacaagaggg ttacaaaatg ttgttgatcc attacctgtg
     4621 tcagaagctg aatttttaac aagatataaa gcgctggtta tctgtgcatt caatgagaaa
     4681 caatcatttg atgctttagt tgaaggaaac ttagaactac ataaaggaac gccatttgaa
     4741 actaaagtta ttgaagcggc aacgttagat ttactaacgg catttttaga tgaacagtat
     4801 cagaaacaag aacaagatta tagtcaaaat tatgcatatg tacgcaaagt aggacatacc
     4861 gttttcaaat gggttgctat cggtatgaca acgttaagtg ttttattaat tgcattctta
     4921 gcctttttat atttttcagt aatgaagcat aatgagcgca ttgaaaaagg ataccaagca
     4981 tttgtaaagg atgattatac gcaagtacta aatacgtatg atgatttaga tggtaaaaaa
     5041 ttagataaag aggcacttta catttatgcc aaaagttata tccaaacaaa taaacaaggt
     5101 ttagaaaaag ataagaaaga aaatttactt aataatgtga caccaaattc aaacaaagac
     5161 tacttattat attggatgga attaggacaa ggacatcttg atgaagcgat taatattgcc
     5221 acttatttag atgataacga tattacaaag ttagcgttga ttaataaatt aaatgagatt
     5281 aaaaataacg gagatttatc gaatgataaa cgttctgaag aaacgaaaaa gtataacgat
     5341 aaattgcaag atattttaga caaagaaaaa caagttaaag atgaaaaagc gaaatctgaa
     5401 gaagagaaag caaaagcgaa agatgagaaa ttaaagcaac aagaagagaa cgaaaagaaa
     5461 caaaaagaac aagcacaaaa agataaagaa aaacgccaag aagctgaaag aaaaaaatag
     5521 tataggactg aggcaaagac aatgcataaa ttgattataa aatataacaa acaattgaag
     5581 atgctcaatt tgcgagatgg taagacatat actattagcg aagacgagcg tgcagatatt
     5641 acgttgaaat cgttaggcga agtcattcat ttagaacaaa ataatcaagg tacttggcaa
     5701 gcgaatcata cttctattaa taaggtgctt gttagaaaag gtgaccttga tgacattaca
     5761 ttacagcttt atacagaagc tgattatgca tcatttgcgt atccttcaat tcaagatacg
     5821 atgacaattg gaccaaatgc gtatgatgat atggttattc aaagcttgat gaatgccatc
     5881 attattaaag attttcaatc aatacaagaa tcacaatacg tacgcattgt gcacgataaa
     5941 aatacagatg tgtatattaa ctatgaacta caagagcaac taacgaacaa agcttacatt
     6001 ggtgatcata tttatgttga agggatatgg ctcgaagtac aagctgatgg tttaaatgta
     6061 ttgagtcaga atacagtggc atcgtcatta attcgcttaa cacaagagat gccacatgca
     6121 caggcagatg attacaatac gtaccatcgt tcgccaagga ttattcaccg tgaaccgacg
     6181 gatgatatta agattgaaag accgccacag ccaatacaga agaacaatac agtgatatgg
     6241 cgttccatta taccgccatt agtaatgatt gctttaactg ttgtcatctt tttagtgaga
     6301 ccaattggta tttatatttt aatgatgatt ggtatgagta cagtaacgat agtatttggt
     6361 attacaacgt atttctctga aaagaaaaag tataacaaag atgttgaaaa acgagagaaa
     6421 gattacaaag cttatttgga taataaatct aaagaaatta acaaagcgat taaagcacaa
     6481 cgttttagtt tgaattacca ttatccaacg gttgctgaaa ttaaagatat cgttgaaacg
     6541 aaagcaccaa gaatatatga aaaaacatcg catcatcacg atttcttaca ttataagtta
     6601 ggtattgcga atgtagaaaa gtcattcaaa ttagattacc aagaagaaga atttaaccaa
     6661 cgtcgtgatg aactattcga cgatgctaaa gaattgtatg aattttacac agatgtagaa
     6721 caagcaccat taatcaatga tttaaatcat gggccaattg catatattgg tgcacgacat
     6781 ctcattttag aagaattgga gaaaatgcta attcaattgt caacattcca tagttatcat
     6841 gatttagagt ttctatttgt gacacgtgaa gatgaagttg aaacattgaa atgggcacgt
     6901 tggttgccac atatgacatt gagagggcaa aacattagag gatttgttta caatcaacga
     6961 acgcgtgacc aaattttaac gtcaatttat agcatgatta aagaacgtat ccaagctgtg
     7021 cgtgaacgca gcagaagtaa tgagcaaatt attttcacac cgcaattagt gtttgtcatt
     7081 acagatatgt cattaattat tgatcatgtc attttagaat atgtaaacca agatttatca
     7141 gaatatggta tttcattaat ctttgttgaa gatgtgattg aaagtttgcc agagcatgta
     7201 gataccatta ttgatatcaa gtctcgtact gaaggcgaac tgattacgaa agaaaaagaa
     7261 ttagttcaat tgaaatttac acctgaaaat attgataacg tcgataaaga atatatcgcg
     7321 cgacgtttgg cgaatttgat acacgtcgaa catttgaaaa atgcaattcc tgatagtatt
     7381 acatttttag agatgtataa cgtgaaagaa gtagatcagc ttgatgtggt taatcgatgg
     7441 agacaaaacg aaacatacaa aacgatggca gtacctttag gtgtaagagg taaagatgat
     7501 attttatcat tgaacttaca tgaaaaagca cacgggccac atggtttagt tgctggtacc
     7561 actggttcag ggaaatctga gattatccaa tcatacattt tatctttagc tattaatttt
     7621 caccctcatg aagttgcatt cctattgatt gactataaag gtgggggtat ggcgaactta
     7681 tttaaagatt tagtccattt agttggtacg attacaaact tagatggcga tgaagcgatg
     7741 cgtgccttaa catcaatcaa agccgaattg agaaaacgtc aacgtttatt cggagagcat
     7801 gatgttaacc atattaatca ataccataag ttatttaaag aaggtattgc gacagaacca
     7861 atgccacatt tattcattat ttccgatgag tttgccgaat taaaatcaga acaacctgat
     7921 tttatgaaag aacttgtatc aacggcacgt attggacgtt cgttaggtat tcatttaata
     7981 cttgcgacac aaaaaccatc gggtgttgtt gatgaccaaa tttggtctaa ctctaaattt
     8041 aagttggcat taaaagtaca agatagacaa gacagtaatg aaattttaaa aacaccagat
     8101 gcagcagaca ttacattacc aggtcgtgcg tatttacaag ttggtaataa tgaaatttat
     8161 gaattattcc aatctgcatg gagtggtgca acatatgaca tcgaaggcga taaattagaa
     8221 gttgaagata agacgattta catgattaat gactatggtc aacttcaagc aatcaacaaa
     8281 gacttgagtg gacttgaaga tgaagaaacg aaagaaaatc aaactgagtt agaagcggtc
     8341 atagatcata tcgaatctat tacaacacga ttagaaatcg aagaagttaa gcgtccatgg
     8401 ctaccaccat tgccagaaaa tgtatatcaa gaagatttag tagaaacaga tttcagaaaa
     8461 ttatggtcag atgatgcaaa agaagtggaa ttaacattag gacttaaaga cgtaccagaa
     8521 gaacaatatc aaggaccgat ggtattgcaa ttgaaaaaag ctgggcacat cgcgttaatc
     8581 ggaagtccag gatatggtag aacaacgttc ttacacaaca ttattttcga tgttgcaaga
     8641 caccatcgtc ctgatcaagc acacatgtac ttgttcgatt tcggtaccaa tggtttgatg
     8701 ccagttacag acataccaca tgtcgctgat tactttacag tagatcaaga agacaagatt
     8761 gctaaggcga tacgtatatt taatgatgaa attgatcgtc gtaagaagat tttaagtcag
     8821 tatcgtgtca ctagtatttc tgaatatcga aaattaactg gtgaaacaat tccgcatgtc
     8881 tttattctta ttgataactt tgacgcagta aaagattcac ctttccaaga agtttttgaa
     8941 aatatgatga ttaaaatgac gcgtgaaggg ctagcattag acatgcaagt aaccttaact
     9001 gcttcaagag ctaacgctat gaaaacacca atgtacatta atatgaaaac gcgtatcgca
     9061 atgtttttat atgataaatc agaggtgtcg aacgtagtag gacagcaaaa atttgcggtt
     9121 aaagatgttg tgggtcgagc attgttaagt agtgatgaca acgtatcatt ccatattggc
     9181 caaccattta aacatgatga gaccaaatca tataatgatc aaattaatga tgaagtatcg
     9241 gcgatgacag aattttataa aggtgaaaca ccaaatgata ttcctatgat gccagatgaa
     9301 attaaatatg aagattacag agaatcatta aacttaccag atatagttgc aaatggtgct
     9361 ttaccaattg gattagatta tgaaggtgtt acactacaaa aaattaaatt aactgaacca
     9421 gcaatgattt catcagaaaa tccgagagaa attgcgcata ttgctgaaat tatgatgaaa
     9481 gaaattgaca tattaaatga aaaatatgcg atttgtatcg cagactcaag tggagagttt
     9541 aaagcttata ggcatcaagt ggctaacttt gccgaagaaa gagaagacat taaagcgatt
     9601 catcaactaa tgattgaaga cttaaagcaa agagaaatgg acggcccatt tgaaaaagat
     9661 tcactttata ttatcaatga ttttaaaaca tttattgatt gcacgtatat tccggaagat
     9721 gatgttaaaa agcttattac aaaaggacca gaacttggct tgaacatttt atttgtcggc
     9781 attcataaag aattaataga tgcttatgat aaacagattg atgttgcacg taaaatgatt
     9841 aaccaattta gtataggtat tcgtatttca gaccaacaat tctttaaatt tagatttatt
     9901 caacgagaac ctgttattaa agaaaatgaa gcatatatgg tcgcaaacca agcttatcaa
     9961 aagattagat ggtttaaata gcaatgaatt aaataggagg gaggtatgtt atgaatttta
    10021 atgatattga aacaatggtt aagtcgaaat ttaaagatat taaaaagcat gctgaagaga
    10081 ttgcgcatga aattgaagtt cgttctggat atttaagaaa agctgaacaa tataagcgat
    10141 tagaatttaa tttgagtttt gcactagatg atattgaaag cacagcaaag gacgtacaaa
    10201 ctgcaaaatc tagtgctaat aaggacagtg taactgttaa gggaaaggcg cccaatacgt
    10261 tatatattga aaaaagaaat ttgatgaaac aaaagcttga aatgttgggt gaagatatcg
    10321 ataaaaataa agaatccctc caaaaagcta aggaaattgc tggcgaaaag gcaagtgaat
    10381 attttaataa agcaatgaat taatattgag gtgaagatat gggtggatat aaaggtatta
    10441 aagcagatgg tggcaaggtt gatcaagcga aacaattagc ggcaaaaaca gctaaagata
    10501 ttgaagcatg tcaaaagcaa acgcaacagc tcgctgagta tatcgaaggt agtgattggg
    10561 aaggacagtt cgccaataag gtgaaagatg tgttactcat tatggcaaag tttcaagaag
    10621 aattagtaca accgatggct gaccatcaaa aagcaattga taacttaagt caaaatctag
    10681 cgaaatacga tacattatca attaagcaag ggcttgatag ggtgaaccca tgatgaaaga
    10741 tgttaagcga atagattatt tttcttacga agaattaaca attttaggtg gtagtaaatt
    10801 gcctctcgta aattttgaat tgtttgatcc atcaaatttt gaagaagcta aagctgcttt
    10861 aattgaaaag gaattagtaa cagagaatga caagttaact gatgcaggtt ttaaagtggc
    10921 tacattagtc agagagtata ttagcgccat tgtaaatatt cgaattaatg atatgtattt
    10981 tgcaccattt agctatgaaa aagatgaata tattttgtta agccggttta aaaataatgg
    11041 gtttcaaata cgaattatca ataaagacat tgcatggtgg tcgattgtac aatcatatcc
    11101 tttattgatg agacaagaaa agtccaatga ttgggacttt aaacaaattg acgatgaaac
    11161 attggagaac ttaaataatg aaagtatcga tacgattggg cgtgttttag aaattgaaat
    11221 atacaatcat caaggtgacc ctcaacaaag tttatataac atttatgaac aaaatgattt
    11281 gttattcatt cgatacccat taaaagataa agtgctgaat gttcatattg gtgtcattaa
    11341 tacatttata cgagaattat ttggattcga tactgatgaa aatcatatta ataaagcaga
    11401 ggagtaatga cgttgagtgg aaaaattagt gttaaagctg aaacgattgc acatgttgta
    11461 aaagaattgg aaagcataag tcaaaagtat gatgaaatag ctcaaaactt tggaaaaata
    11521 gcgcaattaa attactacag tagtgaaaaa gctgcacatt ctatggaaaa tggctatagt
    11581 agtgctgcaa cagtcattag tggtctcaaa ggtccattga gtacactcgg tggtggcgtc
    11641 atgaattcag cacaaaagtt ctttgaagca gatgaacatt ggggtacgga atttgccaag
    11701 ctttactata atattgaggg ataggtgcat gacatgacaa aagatattga atatctaaca
    11761 gctgattatg acaatgaaaa gtcatctatc caaagtgtaa tagatgcaat agagggtcaa
    11821 gacttcttag atgtagatac aacaatggat gatgcggtaa gcgatgtcag ttctttagac
    11881 gaagatggcg caatatcatt aacaagtagt gtagtaggtc cacaaggatc taaattaatg
    11941 gggtattatc aaaatgagtt atatgattat gcatctcaat tagattcgaa aatgaaagaa
    12001 attattgaca cgccatttat agaagatata gataaagcat tcaaaggtat aacgaatgtt
    12061 aaattggaaa atatactaat taaaaatggt ggtggacatg gtagagatac ctatggggct
    12121 tctgggaaaa ttgcaaaggg agatgccaag aaaagtgaca gcgatgttta tagcatcgat
    12181 gaaatattaa aatcggatca agaatttgta aaagtaattg atcagcatta caaagaaatg
    12241 aaaaaagaag ataagaaatt atctaaaagt gattttgaaa aaatgatgac tcagggcgct
    12301 tcttgtgatt acatgacagt agctgaagcg gaagagctag aggagcaaaa gaaaaaagaa
    12361 gaagctatag agattgcagc actagctggt atggtagttt tatcttgtat taatcctgtt
    12421 gctggagcag tagctattgg tgcttattcc gcttattcag cagcaaatgc agccacagga
    12481 aaaaatattg taactggaag aaagctatct aaagaagaac gaatcatgga aggactttcg
    12541 cttattccat tgccaggtat gggcttcctc aaaggtgctg ggaaaagttt aatgaaatta
    12601 ggcttcaaag gcggagaaaa atttgcagtt aaaacaggat tgcaaaagac aatgcaacaa
    12661 gcagttagtc gtatttcacc taaaatggga atgatgaaaa acagtgtgtt gaatcaatct
    12721 cgtaactttg ctcaaaatac tcatgttgga caaatgctga gtaacatgcg tggtcaagca
    12781 actcatactg ttcaacaaag tagaaattgg attggacaac aagcacaaaa tgtcaaacga
    12841 atagtgaata atggacttga taaagaaata gcacatccat ttaaacaaca acttgcacca
    12901 gcgggaatgg gtggtataaa atttgctgaa acaactactt tgagaaacat gggtcaaaac
    12961 ataaagcgtg ctgttacacc acaaaatcac gtgacacatg gtccaaaaga tagtatggtg
    13021 agaagtgaag gcaaacatag tataagtagc catgaaatga attcatcaaa gtatgttgaa
    13081 tcaccaaact acaccaaggt tgaattcgga gaacactatg caagacttag acctaagaaa
    13141 ctaaaggcga atattgaata cacaacacct actggtcaca tatatcgaac cgatcataaa
    13201 ggtcgcataa aagaagttta tgtagacaat ctctctctaa aagatgggga tcgtaatagc
    13261 catgcacaaa gaactgtggg gggagaggat agattaccag acgatgatgg aggtcactta
    13321 atcgctagaa tgtttggtgg ttcaaaagac attgataacc ttgtggcaca aagtaaattt
    13381 atcaaccgtc catttaagga gaaaggtcat tggtataatc ttgagaaaga gtggcaagag
    13441 ttcttaaact ctgggaaaga ggtgaaaaat attaaaatgg aagtaaaata tagcggtaat
    13501 agtcaaagac cgactatatt taaagttgaa tatgaaatta atggtgaaag aaatattaga
    13561 agaatattaa ataagtagag gtgccaacat gacatttgaa gagaagctta gcaaaatata
    13621 caatgaaatt gcgaatgaga tta
//
LOCUS       DAKTGS010000076        13410 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.076, whole genome shotgun sequence.
ACCESSION   DAKTGS010000076 DAKTGS010000000
VERSION     DAKTGS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13410
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.076"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            33..902
                     /locus_tag="O0286_001752"
     CDS             33..902
                     /locus_tag="O0286_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0170130.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVF
                     IKSFKKYIANEQL"
     gene            complement(961..3570)
                     /gene="clpB"
                     /locus_tag="O0286_001753"
     CDS             complement(961..3570)
                     /gene="clpB"
                     /locus_tag="O0286_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCY0170131.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(3773..5587)
                     /locus_tag="O0286_001754"
     CDS             complement(3773..5587)
                     /locus_tag="O0286_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0170132.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            complement(6134..6442)
                     /locus_tag="O0286_001755"
     CDS             complement(6134..6442)
                     /locus_tag="O0286_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCY0170133.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            6556..7380
                     /locus_tag="O0286_001756"
     CDS             6556..7380
                     /locus_tag="O0286_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0170134.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            7432..8748
                     /locus_tag="O0286_001757"
     CDS             7432..8748
                     /locus_tag="O0286_001757"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCY0170135.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(8919..9308)
                     /locus_tag="O0286_001758"
     CDS             complement(8919..9308)
                     /locus_tag="O0286_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCY0170136.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(9637..10539)
                     /locus_tag="O0286_001759"
     CDS             complement(9637..10539)
                     /locus_tag="O0286_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCY0170137.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            complement(10705..>13410)
                     /gene="addA"
                     /locus_tag="O0286_001760"
     CDS             complement(10705..>13410)
                     /gene="addA"
                     /locus_tag="O0286_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCY0170138.1"
                     /translation="VKSDYFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRS
                     KNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFQEILVDEYQDTNRVQEKILSCIK
                     TGDEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTIDGDGTGRRIDLSQNFRSRK
                     EVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYGAPYDESDHPVNLKVLVEADQEHSDLT
                     GSEQEAHFIVEQVKDILEHQKVYDMKTGSYRSATYKDIVILERSFGQARNLQQAFKNE
                     DIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQFKEDELAQIRI
                     LSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYND
                     HYVIQYFSGLIGGRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEE
                     NVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKDFNKRDLKQPVILNQQFGLGMDYFD
                     VDKEMAFPSLASVAYRAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLELE
                     QLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQIEDSSRPNVN
                     ISIVYFEDVSTETILDNDEYRSVNQLETMQNGNEDVKAQIKHQLDYRYPYVNDTKKPS
                     KQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQGKRKANEIGTLMHTVM
                     QHLPFKKERISEVELHQYIDGLIDKHIIEADAKKDIRMDEIMTFINSELYSIIAEAEQ
                     VYRELPFVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMT
                     DEEIGTQLKNKYKIQMKYYQNTLQTILNKEVKGYLYFFKFGTLQL"
BASE COUNT         4430 a         2387 c         1859 g         4734 t
ORIGIN      
        1 gaattattaa cttttttgaa agaggtaagt tcatgaattt agattggtac tatacatttg
       61 tcattttagc gaagacgtta aactatcgat tagctagtga agaaattaat ctcactatac
      121 cttctataca taagcaaatt aaaaatttag aacagcatct caatgtgaaa ctatttgaaa
      181 cttataaaaa tcaaattatc ttgactgaag atggtcatac atttcttcca attgcgcaaa
      241 gttttattga acaatatgaa agtggcatca aacatatcca acttaaaaaa acgatgtttc
      301 aatcgaaatt aaatgttgtc gtgtcttcat atatcgcgac attcattatg cccaaatttt
      361 taaaatcttt ctttaacgag catcctttta tcgatgtttc tcttcatgtc aaaaatgaaa
      421 atattgaaaa agatattaat aatcatacgt atgacatcgg gattagcaga aatcaaccta
      481 aactaagaga agttcattct gaaaaagtat gcgaaggtaa aattgtattg attgctccca
      541 ataaagagaa taatcatctt ctaactgagg catctttatt tgaaaaatac aaaataataa
      601 gtgataacca tccagaatat tggtcatcac ttaaaaataa tattttaaat atttatgaaa
      661 aagcacaatt tctatctatc aatgatgtgc atacttcaat taaattaatc gaaatgaatc
      721 aaggtatatc attcctgcct ctttatataa caacaaatag tgattacaat atttcggtta
      781 ttaacacgaa aattttacaa gcacctattt catttacata tatatacagc aaaaaagaaa
      841 gcccagaaat attggtgttt attaaatcat ttaaaaagta tattgccaat gaacaattat
      901 aataaatttc aaatctaaaa aaccaagaat gcgattaatc atcacattct tggttcaatt
      961 ttattcatga attttttcaa cattaaacgt taagttattg tctgaattta aattaacttt
     1021 aatcgttgta ccttctggga atccctcttt aatcatcata cgtgctaatg gtgtttcaat
     1081 ttggcgttgt acaaaacgtt ttaatggtct tgcaccgtat tgaggttcat aagcttcttg
     1141 acctagccaa gctttagcat catcagaaac ttcaattgag attcgttgtt ctaataatct
     1201 tatatttaat tgcgttaaga ttttatctac aatcatactc atgtcatcaa tagataatgg
     1261 tttaaataat acgatatcat ccatacgatt caaaatttct ggtttgaaat atgcatttaa
     1321 acttgtcata acagcttttt ctgttgattc tgtaatttca ccagtctctt ttacgttttc
     1381 taataaaact tgagatccaa tattacttgt cataataata atagtatttt taaaatcaac
     1441 gctacgtcct ttagaatcag ttaaacggcc ttcatctaaa atttgcaata atacattaaa
     1501 gacgtcagta tgcgcttttt caacctcatc taataaaata actgagtatg gattacgacg
     1561 aaccgcttca gttaattgac ccccttcatc atgtccaata tatcctggag gtgcccctat
     1621 caatcttgat actgcatgtt tttccatata ttcactcata tcaatacgaa tcatatgttt
     1681 ttcagaatca aataatgatg cagctaatga tttagctaat tcagttttac ctactccagt
     1741 tggacctagg aataagaaac taccaatagg tctgtttgga tctttaatac ctgctcttgc
     1801 tctaactact gcatctgaaa ccaggtcaac cgctttatct tgacctacaa cacgtttatg
     1861 caagatgtca cttaagtgaa gtaatttttc acgttctgtt tcaactaatt ttgaaactgg
     1921 tatgcctgtc cattggctga caatatcgcc aatttcttcg tctgttacaa cttcacgaat
     1981 cattcgatct gtatcttcac cttgctcatc ttggaaatta tcctctaatt ctctaagttc
     2041 tttttccaat tgaggaattg ttccatattg tagttcagca gctttttcta aattgttatt
     2101 tgtttgtgca tcttccaacg cttgtctact ttcatctagt tgcgcacgtt tttcttgtaa
     2161 atttgctatt ttttcttttt ctgattctac acgagattga agtgctgctt gtttctcttt
     2221 ttcattggca agctcttctt gtagttcttg taatctctgt ttgctcgcat tgtcagattc
     2281 atttttaagt gcgctttctt caatttctaa ttgcatgaca cgtctattaa cttgatccaa
     2341 ttcagttgga tttgatccca tttccgtacg aattgttgca catgcttggt caactaaatc
     2401 aatcgcttta tctggtaaaa aacgatcagt gatgtaacga tcagacaatt cagcggcagc
     2461 aactaaggct ctatcttgaa tacgcacacc atgatacact tcatatcgtt cttttaaacc
     2521 acgtaaaatt gaaattgtat cttcaacatc aggctcacta actgctactt tttggaaacg
     2581 acgctctaat gccgaatctt tttcaatata ttctcgatat tcatttaaag ttgttgcacc
     2641 aatacaatgt aactctcctc gtgctaacat tggttttagc atgttgcctg catccatggc
     2701 accatctgtt ttaccagcac ctacaagcat atggatttca tcaataaata atataattct
     2761 accatcagac tctttaactt cttttaggac tgcttttaat ctctcttcaa attcaccacg
     2821 atatttagcg cccgctacta atgcgcttaa atctaactca aaaacagttt tatctaataa
     2881 tgattctggc acatctttct taactatacg ttgcgctaat ccttcaacaa ttgcagtttt
     2941 accaacacct ggttcaccaa tgagcacagg gttgttttta gttttacgac ttaaaatacg
     3001 aatcgtattt cgaatttctt catctcttcc tataacagga tccattttac cttgtctaac
     3061 ttcttctact aagtcgcggc catatttagc taatgcttcg taattaactt ctggattttg
     3121 tgatgtcacg tgatttcccc ctcttacttt tttaataatt tctttgataa cttctacttt
     3181 attatttata taatgttttg ttgtttgatc aatgtccatt gccgaacgta aaatatgctc
     3241 cattgaaata tattcatctt catattcttt catgtatgat tcagccttag ttatcaattg
     3301 gtttgcttgt tggctaatat attgaccata ttgtatattg tcaccttcta cagatgcata
     3361 cgtgtttagt ttgtcttcat aagctttgtt taattgatct acctcaatat ttgctcgttc
     3421 taaaatactt ttatataagc tttcactttc atttaaggca gcgcttaaaa ttgcctcaat
     3481 ttctatattt tgtaatttat gttgctgact cagttcaact gcttgttgta aagcactttg
     3541 aacagcatat gtcattttat ttatatccat ataatttcac ctctaatttg tagagttggt
     3601 caagttgcac ttttgacttt gactttcttt gaccttaatt atattatagt cccaatataa
     3661 tcatttatca actcttttac acttaaattt cctaatattt gtaaatattt ttttgaataa
     3721 aaaaatctag ctaatgttac gttacacatt aactagactt attaaacata tgttatttct
     3781 ttttaagtgc ttttaatatt tcatctttta aagctaggac tcctttgtac tctaaatgta
     3841 caccgtctgg tgcaaaatat tcactatgtc cttgtgatcg cttataccaa tcaattaatg
     3901 tgacattgga ctttcgtttc gccgcgtcag ctaataatcg atttacattt gcctcataaa
     3961 ttcttggaac acgtgtatta actaaataaa tcttggcttt tccaaattga ttaagtaaat
     4021 cgtcgagctg tttgacagta aagtcgccat ttgtacctaa ttctaatacg acttgatcag
     4081 atgatttttt atattgtgaa taattcgctt taactaaagg taaggtttga tacaattgac
     4141 gccctacttt tccatcaatt ctagacttag gaactgatga cttaaatgac tcaccgatat
     4201 caaccattac tgaatcaccg attaaaagag gtttaatatt attatatacg tcggaagatt
     4261 cctttttctt ctcacttact aagccatcaa tgtgtatgtt atcaattggt gctatccgca
     4321 ttaaataatt atcttcaatt gtggtatcaa agctattcgc tttttctcca ataatgtctt
     4381 taccatattt attgaatgca cctactaaaa tcaacataaa tggaattaac aaggttacta
     4441 caattgccat tcttataaat tgtggtatat aagaaggtcg ccaatttaaa gctttaatac
     4501 cttcttttct aaatggagtt tctataaagc gatatgatag ctctgcaaat ataattgtta
     4561 aacttatatc tataaagtac acatatacag gtatctgtcc gtctacatag taactatgta
     4621 cgaaactaat tactgcaaaa tgccataaat ataaactata agaccttttc ccgataaata
     4681 ctaacactgg atttgaaaat atcttcgcta tccatgtaga tggatgaacg acactagcaa
     4741 taataaataa cgttaatatg gatattaaat agaaaccacc atcatatatc caattcgtct
     4801 catcattaat aatgaaaaat aataatataa gtactataaa tgataaacta cctatgctat
     4861 caataacata ttttacaact ttaggtggat catttttcaa tttaaacggt ggccataaaa
     4921 aagctaaaat aacacccagt aacaatgtct gtaatctagt atctgtacca aaatacactc
     4981 gtgaatgatc cccattaata ctatagataa acatcattaa ccctaaagaa attattgata
     5041 ctccccaaaa aataaatcct attttgtatc gctttttaat tgttaacaat aatgtaacca
     5101 aaataactgg gaaaaatatg taaaactgtt cttcaattgc taaagaccat aaatgcttta
     5161 atggcataaa tgaaaattgc tcaaaataat taacatcttt tgctatatac caccagtttg
     5221 atacataaaa tatcgcagca ataatatcat gtttaaccct aatgatatta tctgatttta
     5281 ataataaggt tgctgtccct acaaccatta ataaaactat gactgctggt aataaacgtt
     5341 ttaaacgacg tatccaaaag cttttcaatt taatgatacc tgtgtcatca tactctttga
     5401 gtaataagct tgtaattaaa taaccagaga tcacaaaaaa tgtatccaca cctaaaaagc
     5461 cacctgtcaa ccattgctta tttaagtggt aaataataat tcctagaaca gcgattgccc
     5521 tcaaaccatc gagccctgga atatatctca ttttcttata ctttgtaaaa ccctttgttt
     5581 tgttcatttt tgcattcttc ccttttaaaa ctgttcttct tagatgctta attaaattta
     5641 gttatgctgt ttaaaagaat attgaaatgc atatgtatat tattgaatta cgacatcatc
     5701 aaaatcatat tgactaaaat actgttaaat taaaaaaatt accaatgatg attcttactt
     5761 cgaaatccaa tttgtaatgc aactcggcaa cttaaaaact atgaagtatt atgtattgta
     5821 atataactgt aatataaatt aaatttatta taaaaatttt caagaaaata ttcaactaga
     5881 aaatgaattg tacactcttg gaagtgcaag tcactgtctt aattcatatt ttttgaaaca
     5941 agttagatat aaattttcaa aataaaatca gaaactagaa cataaataag gctcccttca
     6001 aaattttcat ttttcaatgt ctactttgaa gggagcttat tcacaatgaa ttatactcta
     6061 caatgttata ttgactgcgg gcccaaacac agagaatttc gaaaagaaat tctacaggca
     6121 atgcaagttt atgttagctc acaccaagtg caatcttagc gtaacgtgac atcatatctt
     6181 ttgtccaagg tggactccat acgatattca cttcagtatc ctgaatttca ggaatctctg
     6241 ctaatactgt tttaacttga tcaataattt gaggtcccat tggacatccc attgatgtta
     6301 aagtcatatc aactgtacat acgccttcat catcaacatt cactttgtat actaaaccca
     6361 aattaacgat atcaattcct aattcagggt caattaccat ttctaatgca cctaagatac
     6421 tatctttcaa tgcctcttcc atccatatca cctctttaat gtcatattat tcataatata
     6481 tcaaatatcc gacaaaacgc caataaaatg ctatgatgta tctatatgaa ctaagcaact
     6541 tatgaggaga gagatatgca accacattta atatgtctag acttagacgg aacattatta
     6601 aacgataaca aagaaatttc atcatatact aaacaagtat taaatgaatt acaacaacgt
     6661 ggacaccaaa ttatgattgc gactggcaga ccttatcgtg caagtcaaat gtattatcat
     6721 gaattaaatt taacgacacc aattgttaat tttaatggcg cttacgtaca tcaccctaaa
     6781 gataaaaact tcaaaacttg ccatgaaatt ttagatttag gcatcgcaca aaacattatt
     6841 caaggattac aacaatatca agtatcgaat attatagcag aagtgaaaga ttatgttttc
     6901 attaacaatc atgatccaag attatttgaa ggtttttcaa tgggtaatcc aagaattcaa
     6961 actggtaatt tacttgtcca cttgaaagaa tcccctacct caattttaat tgaagccgaa
     7021 gaaagtaaaa tacctgaaat caaaaatatg cttactcatt tttatgccga tcatattgag
     7081 catcgacgct ggggcgcacc attccctgtc attgaaattg taaaacttgg tattaataaa
     7141 gcaagaggca ttgagcaagt tagacaattt ttaaatattg accgaaataa tattattgca
     7201 ttcggtgatg aagataatga tattgaaatg attgagtacg cgcgtcacgg tgttgctatg
     7261 gaaaatggtt tgcaagaact taaagatgta gcgaacaata ttacattcaa caataatgaa
     7321 gatggcattg gtcgatattt gaatgatttc tttaatttaa atattagata ttactgttaa
     7381 tttataacta atcattttat aatattttaa aacaatagga ggtaagttac gatgcccaaa
     7441 atagtcgtag tcggagcagt cgctggcggt gcaacatgtg ccagccaaat tcgacgttta
     7501 gataaagaaa gtgacattat tatttttgaa aaagatcgtg atatgagctt tgctaattgt
     7561 gcattgcctt atgtcattgg cgaagttgtt gaagatagaa gatatgcttt agcgtataca
     7621 cctgaaaaat tttatgatag aaagcaaatt acagtaaaaa cttatcatga agttattgca
     7681 atcaatgatg aaagacaaac tgtatctgta ttaaatagaa agacaaacga acaatttgaa
     7741 gaatcttacg ataaactcat tttaagccct ggtgcaagtg caaatagcct tggctttgaa
     7801 agtgatatta catttacact tagaaattta gaagacactg atgctatcga tcaattcatc
     7861 aaagcaaatc aagttgataa agtattggtt gtaggtgcag gttatgtttc attagaagtt
     7921 cttgaaaatc tttatgaacg tggtttacac cctactttaa ttcatcgatc tgataagata
     7981 aataaattaa tggatgccga catgaatcaa cctatacttg atgaattaga taagcgggag
     8041 attccatacc gtttaaatga ggaaattaat gctatcaatg gaaatgaaat tacatttaaa
     8101 tcaggaaaag ttgaacatta cgatatgatt attgaaggtg tcggtactca ccccaattca
     8161 aaatttatcg aaagttcaaa tatcaaactt gatcgaaaag gtttcatacc ggtaaacgat
     8221 aaatttgaaa caaatgttcc aaacatttat gcaataggcg atattgcaac atcacattat
     8281 cgacatgtcg atctaccggc tagtgttcct ttagcttggg gcgctcaccg tgcagcaagt
     8341 attgttgccg aacaaattgc tggaaatgac actattgaat tcaaaggctt cttaggcaac
     8401 aatattgtga agttctttga ttatacattt gcgagtgtcg gcgttaaacc aaacgaacta
     8461 aagcaatttg actataaaat ggtagaagtc actcaaggtg cacacgcgaa ttattaccca
     8521 ggaaattccc ctttacactt aagagtatat tatgacactt caaaccgtca gattttaaga
     8581 gcagctgcag taggaaaaga aggtgcagat aaacgtattg atgtactatc gatggcaatg
     8641 atgaaccagc taactgtaga tgagttaact gagtttgaag tggcttatgc accaccatat
     8701 agccacccta aagatttaat caatatgatt ggttacaaag ctaaataaat gaatcatgcg
     8761 attcaatcta atcttaattg tctaggttag aattatgttg gactggtact actatccagt
     8821 ccatttttta tgtttaacat ttttagaatc aaaaaagaca taaggtcttg gactaataat
     8881 tgtccatgcc ttatgtcata tactatatgt cttatcaatt agccaatacc gaataatttt
     8941 gatataggcc ctaacggtag aatgacacct aataccattg tgatgataat taatgcaatt
     9001 gttatccaaa acattgtgtg actttgttca tgtctctttc ttttagcaat cgacacttcc
     9061 atcaatccaa ctactgcaac accacacagc attttcaatg taagcaacat atgatttgcc
     9121 ccgccattca taaatgactg aattaatatc caaaatcctg aaattaacgt caacagcata
     9181 aataagcgta aaatcatgtg caacggtttg aaaaatggtg atctgccttg attttttgaa
     9241 atgtttaagt atgtagcgat aaataaaata atcgctaata cccaacttaa tatatgtaaa
     9301 tgtaacatac tgattccccc cactttaatt atttatatta ttaaattaaa gcttcttggg
     9361 attaataccc acttgcttgt aatttaatca tgatttgatt atacacgaat atatattcta
     9421 ccacacttct atatttgaga ggaagaacat gacattttat tccttattag aatattgtga
     9481 atctgctgta aaataatcaa ctacttctta atatcaatat ttcactttca tctcaaaatg
     9541 gtaacattat aaataattta tctttaacac ctttttagaa aagcaagaaa aaactaacca
     9601 atctatataa agactggtta gctttttaaa tgataattat ttagcgatat aagttgtcag
     9661 cgttccaata ttatcaatag tcactttaac ttcatcacct ggttgtaaaa atttaggtgg
     9721 ttgcatacct gcaccaacgc ctgctggtgt accagttgca ataatatctc ccggatgtag
     9781 tgcaacatat tttgaaattt cttctattaa ttcatcaatt ttaagaatca tttcgccagt
     9841 gttaccatct tgtctaattt cattgttaac ttttgtaaca atatttacat tttcaggtaa
     9901 tggtagttcg tctttagtaa cgatataagg acccattggg caaccgccag ttaaactttt
     9961 tgataaaaat gcttgatctt gttcactttg tgctttgcga tcagtgatat cgttaataat
    10021 tgtatagccg taaacataat ctaaagctaa tgcttttgga atcttttcac cagacttacc
    10081 aataacaata cctaattcac cttcataatc taattgatca gtaatatctt tatgatttgg
    10141 aattgttgca ttatctcctg ttaaagatga cgctgctttt gtaaatacat ataatttttc
    10201 cacttcatga tttaattcgt tcgcatgatc tttgtaattt ctaccaaaag caatcacatt
    10261 attcggaggt gttactggtg gtaaaaattc aatgtcatta aatgaaattt tatagtcttc
    10321 agctttgccg ctatcttctg ctgctacaac tgctttacgt acttgttctt gaaaatctaa
    10381 agtatgattt tgttgtaaac cagctaacaa tgttttagga tggaaatctc cttctgcaaa
    10441 gtcagcaaat acttgtgtta aatcccatac agcatcttcg cgttttactt taacgccata
    10501 tgaagttttg tcattatact tgaatgataa gaatttcatt cattctcaac tcctcatctt
    10561 tatcttaatt cacattataa cttttttcgt tatcaaataa caaataaata agtaagacaa
    10621 ttttgaaaat gagttgtgtt cattctgcta caaggacttt gcacttaatc gaaattattt
    10681 tttattcttt tgaaaatcaa aatactatag ttgcaatgta ccaaatttga agaagtataa
    10741 ataaccttta acttctttat taagaatcgt ttgaagcgta ttttgataat atttcatctg
    10801 tatcttatat ttatttttta attgtgtacc aatttcttca tctgtcatcc cacggcgacg
    10861 attaaatgca tcggttttat agtctacaaa ataatgcaca ccatctttaa caaagattaa
    10921 gtcaatcata ccttgaataa ttgagacgtc ttcgtctcct tgtggcaatt ggtcaactaa
    10981 tgcttggtta actacaaacg gtaattcacg ataaacttgc tctgcttcag caataatcga
    11041 atataactca ctattgataa atgtcattat ttcatccata cggatatctt ttttcgcatc
    11101 tgcttcgata atatgtttat cgattaatcc atcgatatac tgatgtaact caacttcaga
    11161 tatgcgttct tttttgaatg gtaaatgttg catcactgta tgcattaacg taccaatttc
    11221 attcgctttt cgtttacctt gttcacttag aaatttaggt cgttcatacg ttgaaaaacc
    11281 gatacgatat tgccttactc gttcgtaact tgtgccactt tcttctgttt catattgtct
    11341 tttcaattca gaaacagatt gttttgaggg ctttttagta tcatttacat atggatatcg
    11401 ataatcaagt tggtgtttaa tttgtgcttt aacatcttca ttaccatttt gcatagtttc
    11461 taattgatta accgaacgat attcatcatt atctaaaatg gtttctgtag acacatcttc
    11521 aaagtacaca attgaaatat ttacattcgg acgactacta tcttcaattt gtgctatatc
    11581 tttttcaaat tttaaatcat ctggaattga cgcagattga tgtttagata aaatactata
    11641 aataagatgg aacggatttg gtgaagttaa tcgttcattg acagcaatgt gctcaccaga
    11701 aatagacaat tgctctagtt ctagtaatga tttatcattt ttcactctac caattaaata
    11761 aagttgttct ttcgctcttg ttaatgctac atagactaat cgcatttctt ctgacacaag
    11821 ttctttttcg gcaacagctc tatatgcaac cgaagctaaa gatggaaatg ccatttcttt
    11881 atccacatca aaataatcca ttccgagacc aaattgctga tttaaaataa ctggttgttt
    11941 caaatcacgt ttattaaaat cttttgacaa tccagaataa atgacaaatg gaaactctag
    12001 acctttacta ctatgaattg tcatcattct aacgacatta tcgtttggac caactacatt
    12061 ttcctcacca aaatctttgc ctctttcaat caattcatcg ataaaacgaa taaattgata
    12121 taaacctcta aaacttgaat tctcaaactc gatagcttta ttaaataaac cataaagatt
    12181 tgcacgtcgt ccacgtccac caataagtcc actaaagtat tgaataacat aatgatcatt
    12241 ataaaattta tcaattaact gatacaccgg atgatcttta ctatattgtt ggtaactttg
    12301 aatatctgat aaaaacattt ttaatttatc aactaaaatt gcatctgctg cttcgtcatt
    12361 aatgtaattt acaatcgatt gatagaagta gtcatcattt ggactcaata ttctaatttg
    12421 agctaattcg tcttctttga actgatatat aacggagcgc attaacccaa ctaaataaat
    12481 atcttgtaat ggattatcta tcgctcttaa aaatgataat actaagcgga cttctgtttg
    12541 ttcaaagtaa ccttcacgac tattcacatg gaatggaata tcttcatttt taaaggcttg
    12601 ttgtaaattg cgagcttgtc caaagctgcg ttctagaata acgatatcct tgtatgtcgc
    12661 acttctatag cttcctgttt tcatatcata aactttttga tgttctaaga tatctttaac
    12721 ttgttctact ataaaatgcg cttcttgttc actaccagtt aaatcactat gttcttgatc
    12781 cgcttcaaca aggactttta agtttactgg atggtccgat tcatcatatg gtgcaccata
    12841 atacaactgt gccgcttcat catattttac ttcaccgact tgttcatcca tcatatgttt
    12901 gaatatatag ttagtcgttg acagtacttc ttttcgagaa cggaagtttt gcgacaaatc
    12961 aattcgacgt ccagtgccat ctccatctat agtaaagcgt tgatactttt caataaataa
    13021 acttggatca gcttgtctaa atttataaat ggattgctta acatctccaa ccataaataa
    13081 attaccatta tgttcatcac ccgttttgat gcaagatagt attttctctt gaactcggtt
    13141 cgtatcttga tactcatcga ccaatatttc ttggaagtgt tgacggtatg attcggcaat
    13201 ttctgaaggc gaaccatcct cattagttaa aatttgtaat gcaaaatgtt cataatcaga
    13261 aaaatccaaa atatttttgc tacgcttttt tcgattgaat tctgacataa catctttcac
    13321 aatacgcgca aggtacttta ctcgtggcgc caattgttgc atatcagctt tcaaatcatc
    13381 agcttctctt gaaaagtaat cactcttcac
//
LOCUS       DAKTGS010000077        13338 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.077, whole genome shotgun sequence.
ACCESSION   DAKTGS010000077 DAKTGS010000000
VERSION     DAKTGS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.077"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            117..7553
                     /locus_tag="O0286_001761"
                     /pseudo
     CDS             117..7553
                     /locus_tag="O0286_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(7872..8978)
                     /locus_tag="O0286_001762"
     CDS             complement(7872..8978)
                     /locus_tag="O0286_001762"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0170139.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(8978..9412)
                     /locus_tag="O0286_001763"
     CDS             complement(8978..9412)
                     /locus_tag="O0286_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0170140.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMENGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(9424..11556)
                     /locus_tag="O0286_001764"
     CDS             complement(9424..11556)
                     /locus_tag="O0286_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0170141.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            complement(11591..13129)
                     /locus_tag="O0286_001765"
     CDS             complement(11591..13129)
                     /locus_tag="O0286_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCY0170142.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
BASE COUNT         5109 a         2363 c         2225 g         3641 t
ORIGIN      
        1 aattttaatt agatagagtt gctttgtgtt tttaacgcag atgctactac ttatcttaac
       61 agttgattaa gtgaaatcat ttaacagcga gaataatcaa ccaggaggat gacttaatga
      121 atttattcag acaacaaaaa tttagtatca gaaaatttaa tgtcggtatt ttttcagctt
      181 taattgccac tgttactttt atatctacta acccgacaac agcgtctgca gcagagcaaa
      241 atcagcctgc acaaaatcaa ccagcacaac cagctgatgc caatacacag cctaacgcaa
      301 atgctggtgc tcaagctaat cctacagcac agccagctgc acctgccaac caaggacaac
      361 cagcagtaca accagcaaac caaggtggac aggctaatcc agcaggagga gcagcacaac
      421 caaatacaca accagctgga caaggtaatc aagctgatcc gaataacgct gcacaagcac
      481 aacctggaaa tcaagcaaca ccggcaaacc aagcaggtca aggaaataac caagcaacac
      541 ctaataataa tgcaacaccg gcaaatcaaa cacagccagc gaatgctcca gcagcagcgc
      601 aaccagcagc acctgtagca gcaaacgcac aaactcaaga tccaaatgct agcaatactg
      661 gtgaaggcag tattaatacg acattaacat ttgatgatcc tgccatatca acagatgaga
      721 atagacaaga tccaactgta actgttacag ataaagtaaa tggttattca ttaattaaca
      781 acggtaagat tggtttcgtt aactcagaat taagacgaag cgatatgttt gataagaata
      841 accctcaaaa ctatcaagct aaaggaaacg tggctgcatt aggtcgtgtg aatgcaaatg
      901 attctacaga tcatggtaac tttaacggta tttcaaaaac tgtaaatgta aaaccagatt
      961 cagaattaat tattaacttt actactatgc aaacgaatag taagcaaggt gcaacaaatt
     1021 tagttattaa agatgctaag aaaaatactg aattagcaac tgtaaatgtt gctaagactg
     1081 gtactgcaca tttatttaaa gtaccaactg atgctgatcg tttagattta caatttattc
     1141 ctgacaatac agcagttgct gatgcttcaa gaattacaac aaataaagat ggttataaat
     1201 actattcatt cattgataat gtaggtctat tctcaggatc acatttatat gtcaaaaata
     1261 gagatttagc accgaaagca actaacaata aagaatatac tattaatact gaaatcggta
     1321 acaatggtaa ttttggtgct tcattaaaag cagatcaatt taaatatgaa gtaacattac
     1381 cacaaggtgt aacttacgtt aataattcat taactacaac attccctaat ggtaatgaag
     1441 acagtacagt attgaaaaat atgactgtta attatgatca aaatgcaaat aaagttacat
     1501 ttacaagcca aggtgtgaca acggcacgtg gtacacacac taaagaagtt ttattcccag
     1561 ataaatcttt aaaattatca tataaagtta atgttgcgaa tatcgataca cctaaaaata
     1621 ttgattttaa tgaaaaatta acatatcgta ctgcttcaga tgttgtaatt aataatgcgc
     1681 aaccagaagt tacactaact gcagatccat tttcagtagc ggttgaaatg aacaaagatg
     1741 cgttgcaaca acaagtaaac tcacaagttg ataatagtca ttacacaaca gcatcaattg
     1801 cagaatacaa taaacttaaa caacaagcag atactatttt aaatgaagat gcgaatcatg
     1861 ttaaaactgc aaatcgtgca tctcaagcgg atattgatgg tttagtaact aaattacaag
     1921 ctgcattaat tgataatcaa gcagcaattg ctgaattaga tactaaagct caagaaaagg
     1981 ttacagcagc acaacaaagt aaaaaagtta cgcaagatga agttgcagca cttgtaacta
     2041 aaattaacaa tgataaaaat aatgcaatcg ctgaaattaa taaacaaact acagcacaag
     2101 gtgtcacaac tgaaaaagat aatggtatcg cagtgttaga acaagatgtg attacaccaa
     2161 cagttaaacc tcaagcgaaa caagatatta tccaagcagt tacaactcgt aaacaacaaa
     2221 ttaaaaagtc aaatgcatca ttacaagatg aaaaagatgt agcaaatgat aaaattggta
     2281 aaattgaaac aaaggcaatt aaagatattg atgcagcaac aacaaatgca caagtagaag
     2341 ccattaaaac aaaagcaatc aatgatatta atcaaactac acctgctaca acagctaaag
     2401 cagcagctct tgaagaattt gacgaagttg ttcaagcaca aattgatcaa gcacctttaa
     2461 atcctgatac aacaaatgaa gaagtagcgg aagctattga acgtattaat gcagctaaag
     2521 tttctggtgt taaagcaatt gaagcgacaa cgactgcaca agatttagaa agagttaaaa
     2581 acgaagaaat ctcaaaaatt gaaaatatta ctgactctac gcaaacaaaa atggatgcct
     2641 ataatgaagt taaacaagct gcaacagcta gaaaagctca aaatgctaca gtttcaaatg
     2701 caacaaatga agaagtagca gaagctgatg cagcagtaga tgcagctcaa aagcaaggtt
     2761 tacatgacat ccaagttgtt aaatcaaaac aggaagttgc tgatacaaaa tcaaaagtat
     2821 tagataaaat caatgcaatt caaacacaag caaaagttaa acctgcagct gatacggaag
     2881 tagaaaacgc atataataca cgtaaacaag aaattcaaaa tagcaatgct tcaactacag
     2941 aagaaaaaca agctgcatat acagaattag atactaaaaa gcaagaagca agaacaaatc
     3001 ttgatgctgc aaatacaaac agtgatgtaa caacagctaa agacaatagt attgctgcaa
     3061 ttaatcaagt tcaagctgcc acaactaaga aatcggatgc aaaagcggaa atcgctcaaa
     3121 aagcaagtga acgtaaaaca gcaattgaag caatgaatga ttcgactact gaagaacaac
     3181 aagcagcgaa agacaaagtg gatcaagcag tagttactgc aaacgctgat atagataatg
     3241 ctgcagcaaa caatgatgtg gataatgcaa aaactacaaa tgaagctaca atcgcagcca
     3301 ttacacctga tgcaaatgtt aaaccagcag caaaacaagc aattgcagat aaagtacaag
     3361 ctcaagaaac agcaattgat ggaaataacg gctcaacaac tgaagaaaaa gcagctgcta
     3421 aataacaagt tcaaactgaa aaaacaacag ctgatgccgc aatagatgca gcacatacaa
     3481 atgcggaagt tgaagcggct aaaaaagcag caattgctaa aattgaagcg attcagccag
     3541 caacaacaac taaagataat gcgaaagaag caattgctac gaaagcgaat gaacgtaaaa
     3601 cagcaatcgc tcaaacgcaa gacattactg ctgaagaaat tgcagcggct aatgcggacg
     3661 tagataatgc tgtgacacaa gcaaatagca acattgaagc tgctaatagt caaaatgatg
     3721 tagaccaagc gaaaacgaca ggtgaaaata gtattgatca agtaacacca acagttaata
     3781 aaaaagcaac tgcacgtaat gaaatcacag caattttaaa taacaaattg caagagattc
     3841 aagctacgcc agatgcaaca gatgaagaaa aacaagcagc tgatgctgaa gcaaatactg
     3901 aaaatggtaa agcaaatcaa gccatttcag cagcaactac taacgcacaa gttgatgaag
     3961 ctaaagcaaa tgcagaagca gcgattaatg cggtaacacc aaaagttgtg aagaaacaag
     4021 cggctaaaga tgaaattgat caattacaag caacgcaaac aaatgttatc aataatgatc
     4081 agaacgctac aacagaagaa aaagaagcag ctattcaaca attagcaaca gcagttacag
     4141 acgcgaaaaa taatattaca gctgcaactg atgataatgg tgtagatcag gcgaaagacg
     4201 ctggaaagaa ttcaattcaa agcacgcaac cagcaacagc ggttaaatca aatgctaaaa
     4261 atgatgttga tcaagctgtg acaactcaaa atcaagcaat tgataataca actggtgcta
     4321 caactgaaga gaaaaatgca gcaaaagatt tagttttaaa agctaaagaa aaagcgtatc
     4381 aagatatctt aaatgcacaa acaactaatg atgttacgca aattaaagat caagcagttg
     4441 ctgatattca aggtattact gcagatacaa caattaaaga tgttgcgaaa gatgaattag
     4501 caacaaaagc aaacgaacaa aaagcgctta ttgcacaaac tgcagatgcg actactgaag
     4561 aaaaagaaca agcaaatcaa caagtagacg cacaattaac acaaggtaat caaaatattg
     4621 aaaatgcaca gtcaatcgat gatgtaaaca ctgcaaaaga taatgcaatt caagcaattg
     4681 acccaattca agcatcaaca gatgttaaaa cgaatgcaag agcggaattg ctaactgaaa
     4741 tgcaaaataa aataactgaa atacttaata ataatgagac tactaatgaa gaaaaaggta
     4801 acgatattgg accagttaga gcagcatatg aagaaggttt aaataatatt aatgcagcaa
     4861 ctactacagg tgatgtaact actgctaaag atacagcagt acaaaaagtt caacaacttc
     4921 atgcaaatcc tgttaagaaa ccagcaggta aaaaagaatt agatcaagct gcagctgata
     4981 agaaaacaca aatagaacaa acaccaaatg catcacaaca agaaattaat gatgcaaaac
     5041 aagaagttga tactgaatta aatcaagcga aaacaaatgt cgatcaatca tcaacaaatg
     5101 aatatgttga taatgcagtt aaagaaggaa aagctaaaat taatgcagtt aaaacattta
     5161 gtgagtacaa aaaagatgct ttagctaaaa ttgaagatgc atataatgct aaagtaaacg
     5221 aagcggataa ctctaacgca tcgacttcaa gtgaaattgc tgaagcgaaa caaaaacttg
     5281 ctgaattaaa acaaactgcg gatcaaaatg ttaatcaagc tacttctaaa gatgacattg
     5341 aagttcaaat tcataatgac ttagataata ttaacgatta cacaattcca acaggtaaaa
     5401 aagaatcagc tacaacagat ttatatgctt atgcagatca gaagaaaaat aatatttcag
     5461 ctgacactaa tgcaacacaa gatgaaaagc aacaagcaat taagcaagtt gaccaaaatg
     5521 ttcaaactgc attagaaagc attaataatg gtgtggataa tggtgacgtt gatgatgcat
     5581 taacacaagg taaagcagca attgatgcta ttcaagtaga tgctactgtt aaacctaaag
     5641 cgaaccaagc tattgaagtt aaagcagaag atacgaaaga atctattgat caaagtgacc
     5701 agttaactgc tgaagaaaaa actgaagcat tagcaatgat taaacaaatt acagatcaag
     5761 ctaaacaagg tattactgat gcaacaacaa ctgctgaagt tgaaaaagcg aaagctcaag
     5821 gacttgaagc atttgataac attcaaatcg actcaacaga aaaacaaaaa gctatcgaag
     5881 aattagaaac tgcactagac cagattgaag caggtgtaaa tgtcaacgct gatgctacaa
     5941 ctgaagaaaa agaagcgttt acgaatgctt tagaagacat tttatcaaaa gcaactgaag
     6001 atatttctga tcaaactaca aatgcagaaa tcgctactgt caaaaatagt gcgcttgaac
     6061 aacttaaagc acaacgtatt aatcctgaag ttaagaaaaa tgctttggaa gcaatcagag
     6121 aagtggttaa caagcaaata gaaataatta aaaatgcaga tgcagatgca tcggcgaaag
     6181 aaattgcacg tacggattta ggtagatatt ttgacagatt tgctgataaa ttagataaaa
     6241 cacaaacaaa tgcagaagtt gctgaattac aaaatgtaac gatacctgca attgaagcga
     6301 ttgtgcctca gaatgatcca gatgctaatg atactaataa tggtatagat aataatgatg
     6361 caacagcaaa ttcaaatgct aatgcaacac cagaaaatac tggacaacct aacgtatctg
     6421 agacgacagc taatggcaaa gcagacgctt ctccgacaac accaaataat agtgatgcgg
     6481 caacaggtga aacaacagca acatcagcaa ctgatgatgc aaatgataaa ccgcaagcta
     6541 ataacaattc tagtgtagat gcatcaacaa acagtccgac aatggataac gatgtaacta
     6601 gtaaaccaga agtagaatca actaataacg gcacaactga taaacctgta acagaaactg
     6661 ataatgcaac gccagcagaa agcacaacaa ataacaatag tacaactaca gcaacaaacg
     6721 agaatgcacc aacaggatca acagcaacgg ctccaacaac tgcgagcaca gaagcagcat
     6781 catctgctga tagcaaagat aatgcatctg taaatgattc aaaacaaaat gctgaagtaa
     6841 ataatagtgc tgaatctcaa tcaactaatg acaaggttgc acaaccaaaa tctgaaaata
     6901 aagctaaggc agaaaaagat ggtagtgatt caacaaatca aagtatggtt gaatcaacaa
     6961 ctgaaacatt gccttcagca gacataacag aaccaaatgt accttctaat acatcaaaag
     7021 ataaagaaga aagtacgaca aatcaaactg atgcaggaca acttaagtct gaaactaacg
     7081 ttgcatctaa cgaagcagat aaatcgccaa gcaaagctga tactgaagtt tcgaataaac
     7141 catcaacatc tgcatcttct gaagcaaaag aaaaaatgac ttcaactaat gttagccaaa
     7201 aagatgatac ggcaacagca gatactaatg atacgcaaaa atcagttggt tcagctgcaa
     7261 acaataaagc tacgcaaaac gacggtgcca atgcatctcc agctacagtt tcaaatggaa
     7321 gcaatagtgc taatcaagat atgcttaatg taactaacac tgacgaccac caagctaaga
     7381 caaaatcagc tcaacaagga aaagttaata aagctaaaca acaagctaaa actttaccag
     7441 atactggtat gtcacataat gacgatttac catatgctga attagctcta ggtgcaggta
     7501 tggcattctt aattagaaga ttcactaaga aagaccaaca aactgaagaa taaaatttat
     7561 tcaagaatgt gaacatctag ttatttcata gggaaatatt ccttactaag taaccaagtc
     7621 taacagttaa tatgaatttg ttttagtaat gaccaatagc cattgaatag gtggctgtgg
     7681 ctcgaaaata aaacaaatta ttaacgcaaa aatactcctt tcatacataa cacaagttaa
     7741 gtgtctatgt tgaagggagt attttttatg ttcagaaatt taatattgtt aattcaaggg
     7801 aactactaca ttattactga ttgttaacga ataacgttgg aaacttcttg tttcaaacaa
     7861 agctcaataa attatgaaag tgaattgtac gcttcaatta tttcatcggc taagccatcg
     7921 tcaactttag catattcact taaaaacgct tttaaaccgt gttctttaat gtaattattt
     7981 ttctcaacag tttcttggtc tgctgtatca tcatacttca ataacaatgc agctgtattt
     8041 aataaaccag tgcgttctaa atctttatta taaagatatg ttaatggttt tataattcta
     8101 tctttagggc caattttacg taatgtacct cgtccgacac gtgttacttc atcagataaa
     8161 taagaattgt tgaaacgatc tattattttt tcaacataag cagcttgttc cgcttcagta
     8221 aaatcaaatt cattagtaat atattgacta gtttcagcta aaacacggcg taagccagct
     8281 tcaattgaac tatcttcaac tgcatctaaa actgtagctt tacctgcaaa tttaccagca
     8341 tacgctaaat atgcatgtcc tgtattcaca gttaataatt tacgctcaat atatggtgtt
     8401 aaatcatcaa catatttaat atggtttagt tctggaccat accatgcatc tttttcaaca
     8461 acccattcgt aaaatggctc aaccataacg tctaatatat tttcattctt ttgtaatggt
     8521 acaattctat caactgctga gttagcaaaa tgaatattgt taccaagagg gccagtaata
     8581 tcaagtactg cttttttcaa tgtatcagtt gccataatag cattctcaca agcaacaata
     8641 ttaacatggt ttgttttttc ttttaagaaa ggcgcaaaag atttagcaat aataggtagt
     8701 atgttaacac caacagctgt tgtaataata tcagcttcta gaattgcttg tttcaacgct
     8761 tcagaaggtt gcattgaatt aattgcatca acattattca cgcgcgtcgt tgttttagac
     8821 tcatcagcta aaataacatc gtattgatga tcatgagcta aagcattaat gatttcttca
     8881 ttgacgtctg caaatgttac tttaacattg ttgtctgcaa gaatataacc aatgaaacca
     8941 cgacctatgt taccagcacc aaagtgaact gctttcatta tgcatctgcc tcctcgaata
     9001 cttgtttaat ttcttctgca gattttgctt gaacgatacg atccacattt tcttcttcgc
     9061 taaatgtaat tgcaatttta gataacaagt ctaaatgttc gccatcttta ccagcaattc
     9121 ccacgacaac tttaactact tcgccatccc agtcaacgcc ttcagggatt tgtaataatg
     9181 ttaaacctga gtgaatcaca tttgttttag cttcatctgt gccatgagga attgctaagc
     9241 cattttccat aaatgttgat acgacttgct cacgatcttt cattgcttga atataagcat
     9301 ctgttacagc accactatca actaaggctt tacctgcttt ttcaattgct tcattttggc
     9361 tgttaacatt tacatttaaa aagatattgt cattactaaa taattcgctc attttgtaca
     9421 ctcctaaata aattgttttt taattcgttc taaaaatttg tttcttaata atgtttcaag
     9481 ttcctgtgga ttttccataa aagtatcaat agattctaat ttcaaactta aaaattcgga
     9541 caagtcactt accagtgatg ccataaaatc attgtcagaa ataaacatgg aaatcatata
     9601 tttaattgtt tgattgtcat tttgaatact aggcaatgtt aacggttctt ctaaaactgt
     9661 gattagaatc atagggtgtt taatcatatt gtctcttagg tgtggtattg caacaggata
     9721 tggacttaat atccatcctg gattgtcaat caatcgttgc tctagtagtt cagcgaatga
     9781 cgttggatcg tttattacat cgcattgata tagataatct gcgatatatg tttgccactg
     9841 tttaacttcg gtactaacta ttttcactga atcaagcaaa gttaaacccg aacgcatatg
     9901 atgcaataat tgttctgatt tttcgtcact taaatgtttt tcgaaaactg aagttaagta
     9961 tgaagtttgt tcattttcaa aagatgtgtt gccatcaaca tctttcgttt caacatgaac
    10021 atcatcctta tcaatcatgt ttgatgattt atcatgcgtc tcttggaacg tagactttgt
    10081 atttaaaaac tgtgcgacat aactgatatc actatctggt aataatgggt ttaccgttaa
    10141 atagggggag tcgatgtcta aattcacagt agaaataatg ccatcatatt gacttaaatc
    10201 tagtgacttc aaatcgctga ctgatgcttg tgtaatacgc tcaatctcac taaaaacttg
    10261 atctagacga gttgataata gacgactagt tcccatacca ctgctgcaaa cgactaatat
    10321 gtttaaaaat cgattacctt ggtttttaat cgagccacca aagtgtaaaa ctataaaagc
    10381 aatttcactc tctggaaaaa tcaaatcagg ccaacaatca tttaaagcta atcttacatt
    10441 ttcaaatagt cttggatatt tgaacttaat catgtctgtt aacggattat aggtctcgat
    10501 attagcttgc aaacgattga ttgcaggatt aaggtgtagc gtcagtccat ctactaaagt
    10561 atccaaatct gcaaatgtca taccggaaat catttctact ttgttaacaa acgctttaat
    10621 tttgttttct tcacaacgat tatttaatga tgtatcatta agattttttc gtttagctcc
    10681 acgtaaatgg atagtaatga aagcaatttc tgcctgatta aacgtgacgt catatatttg
    10741 accaagttta tcagcaagtt cgcttgctac tttgtgttca aatgtgtttt gtacagaatc
    10801 ataaatatca tcgtttaatg cgacatactc tccatttttt atacgtgaaa tggagagcac
    10861 gatatggaca gttaaagtta aataacttga ttcggtaagt tggtagggta aggcacttag
    10921 gtagtccatt aatagtcttt caacattaaa aatgcgctct aagtcaacaa agtctttgtg
    10981 gatttgattt aatgaatgaa agacaaaatg attttcaatt actgaataaa cgctagtact
    11041 atttaagtta ttcaccatta attgacttaa aaattcacgt ttctttgatt cagtacctac
    11101 caagtaaatg ccttcgccac gctttcgaga taaagatagt tggtacttat ttaaatcaag
    11161 ctctaaatca tctaacatct ttgctaaagt ctggacagaa acgccaattt cttgcgctaa
    11221 actatattgt ttaactggct ccttggcttg tatcaaagcg tatataataa ttactttctg
    11281 ctcttcaaca gataagtcaa tggtttgatg ttgtgcaatc gattgcttca aatcgtttaa
    11341 atcagaatct gtgccagcaa tgcgtagccc ttttttgttt gcgcgttcta aagttaatga
    11401 aaatgaagtt aaatatgctt caacaccttt taattcacgg tgaatagtac gagaggacac
    11461 cgcaagttgc tgagcaatgt cgtgtatagt gatatattga ccgtggtact taatcaatag
    11521 ggctatcatt tctttttcac gtgtactcaa taacatggcg gtatcctcca ttttttaatt
    11581 tatttaatta ttatgcttga tcatcttttt ttagattatt taaaagttct tcatatcttg
    11641 gtgaattaag gaaattatcc actgaaatat ggatggcatt tggtgtttgt ttaatagcac
    11701 gatcagttag ttttttctga gtgataacta attgagcatc ttttggcaat tgattaatcg
    11761 cagtatttgt aactgtaata tcattaatgc ccgccttttt aaatttatta cgtaacatgc
    11821 ttgcacccat tgcactagaa cccatacccg catcgcaggc aaaaataaca tgatttatat
    11881 tattgtaatt gtgtgcatca acatcttcag tgttgtaatt atccaatagc gcttcaggat
    11941 catcatctga tgaagatgtt tcactaacat taccactagc attttcttct gtattaacat
    12001 ttttatcaga agatactaac ttagaagcaa cgcttgattt tttcccttta gtattttcca
    12061 tttgagctgt cgcagcttct aaatcctgct ttggttctct agtgaacttc ataattaaag
    12121 cagctacaac gaatgataca agtgcagcaa ggaagacacc gagtaacatg tgcaagaatt
    12181 cacctctagg tgcatttaaa cagtaaacta taaatgaacc tggtgacgcg ggacttttaa
    12241 atccaaatcc tgttgcttga taagttgcaa caccagtcat tccacctaaa ataacagcga
    12301 taaataataa aggacgcatt aatacatatg ggaaataaat ttcatgaata ccacctaaga
    12361 agtggataat tccagcacca tatgacgttg cttttgcagt gccttttcca aaaatcatat
    12421 aagcaagtaa gatacctaaa cctggtccag ggttagattc aattgtgtat aaaattgatt
    12481 gaccagcttt tgcagcttga tctgcaccaa gcggtgtgaa tacaccatgg ttaatcgcat
    12541 tgtttaaaaa tacaattttt gcaggctcta ctaaaatact tacaagtgga agtaggtgtg
    12601 catgtactaa tgcttcaact gccactgata aaatatgcat aataaatttc ataagtggtg
    12661 ctaaaatttt aaatcctgca atcgtcatga taaatcctaa aataccagca gaaaagttat
    12721 taaataacat ttcaaaacct tgcggcgttc taggttgaat caattggtcg gtcttcttca
    12781 ttaaccaacc aacaagtgga cccataatca ttgcaccaag taacattggt gtatcaggta
    12841 atgcaacgat gacccccata gttgctgttg ctgcgatgat accaccacgt aaatcataaa
    12901 ttaaacgacc accactaaat gcgatcaata atgggattaa ataagtaatc attggtcctg
    12961 ctaaagtagc taaatctttg ttaggtaacc atccattatc tataaaaatg gccgcgataa
    13021 aaccccaagc gatgaaagcg ccaatgtttg gcatgatcat actacttaag aatgatccaa
    13081 atgcttgaac acgacgacca attccttttt tctcttcagt ttgtgacatg tttacacctc
    13141 ttttttaata tatgtgacta tatatagtgt atagagaggt tacttgttac tcaatataaa
    13201 caaaaatcaa ctttgtcaaa ataaatgtga caaaattaaa taaagtgtca tcaatgtgac
    13261 agtatagata ttttgaaaaa gtaaaacaaa aaaattgttt taggattttt aaaattttat
    13321 tgtgaaaata tttgcaaa
//
LOCUS       DAKTGS010000078        13090 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.078, whole genome shotgun sequence.
ACCESSION   DAKTGS010000078 DAKTGS010000000
VERSION     DAKTGS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.078"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..1803)
                     /locus_tag="O0286_001766"
     CDS             complement(<1..1803)
                     /locus_tag="O0286_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCY0170143.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIE"
     gene            complement(1827..2798)
                     /locus_tag="O0286_001767"
     CDS             complement(1827..2798)
                     /locus_tag="O0286_001767"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCY0170144.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(2785..3987)
                     /locus_tag="O0286_001768"
     CDS             complement(2785..3987)
                     /locus_tag="O0286_001768"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCY0170145.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(3992..5134)
                     /gene="bshA"
                     /locus_tag="O0286_001769"
     CDS             complement(3992..5134)
                     /gene="bshA"
                     /locus_tag="O0286_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCY0170146.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(5375..5692)
                     /locus_tag="O0286_001770"
     CDS             complement(5375..5692)
                     /locus_tag="O0286_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0170147.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(6029..6709)
                     /locus_tag="O0286_001771"
     CDS             complement(6029..6709)
                     /locus_tag="O0286_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCY0170148.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(6781..7368)
                     /locus_tag="O0286_001772"
     CDS             complement(6781..7368)
                     /locus_tag="O0286_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCY0170149.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(7358..7933)
                     /locus_tag="O0286_001773"
     CDS             complement(7358..7933)
                     /locus_tag="O0286_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCY0170150.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            complement(7947..9191)
                     /locus_tag="O0286_001774"
     CDS             complement(7947..9191)
                     /locus_tag="O0286_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170151.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(9198..10496)
                     /gene="aroA"
                     /locus_tag="O0286_001775"
     CDS             complement(9198..10496)
                     /gene="aroA"
                     /locus_tag="O0286_001775"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCY0170152.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(10506..11570)
                     /locus_tag="O0286_001776"
     CDS             complement(10506..11570)
                     /locus_tag="O0286_001776"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCY0170153.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(11596..12762)
                     /gene="aroC"
                     /locus_tag="O0286_001777"
     CDS             complement(11596..12762)
                     /gene="aroC"
                     /locus_tag="O0286_001777"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCY0170154.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         4171 a         2432 c         1747 g         4740 t
ORIGIN      
        1 ttcgatattt ttaaatttat cttttaaata caacaactgt ttccgtaatg atttaactgt
       61 tttatgacta atgccattga atatttctag cgttttattt aacttatcga taatcgcatg
      121 taaatccttc aaaatgtctt ttgtttcaaa agtaaataca ttatggaagc gatgaatatc
      181 atcatcataa acatcagaat cattgataat cgtaaatatc gttgagaaca attgctcatt
      241 taactcatga atctcattca tactagcctt caagccaaaa atatcaattg gtgcaatatc
      301 taatttttcc aaaattcgct gcttttccag ttgatcaatt gcctttaaca atttttcatt
      361 ttcgttttta ccaatcaaac caagctgata tttaatatca gcataactca actcatttgt
      421 cacttgattt aaggcatagt ctggtaagcg atgtgcttca tccactatac aatcatcaaa
      481 caattgatat attgaatttt caacatcaga atgaattaaa tgtgcatgat ttgtaatacc
      541 aatttgaatg ttctgtgcat ttcgcttaat aaaattataa taatgaacat cgtgacgtgc
      601 cggtacatat gtttcaattt tctggtcaaa atacatcttt tgaccacctt ttaaatttaa
      661 ttcctgtata tctccggacg gcgtctctgt aatccaaatc agcaattgca ttttcaagat
      721 attcacttcg taattacttg tgtcatcttt taaaatttga ctaataagcc ccaatgaaat
      781 gtaatcactt ttacttttaa tcaatagtgc attaatttta aaattcaacg cttcattcat
      841 tgctggaata tctttttcta acaattgact ttgcagtaat ttagtattgg tagaaatcat
      901 gacatgcttc ccagtttcaa tattatacat caaggccgca agtaaatatg ctaatgattt
      961 accactgcct agtgatgctt caatcattgc tttttcacta tgcatgagct gatctaatat
     1021 agtttccgct aaatataatt gttgcggtcg atatgttaag ccaagttgat ctacagcttt
     1081 gctatataaa gacttcaagc tgccattata atttgttgtc ggctttttaa aatcaacttg
     1141 cttacgatag ataatctgtt cgaacttttc gtacgattta tccaatggct ttgcatcata
     1201 ttgcctaacc atctcaaaga aaatatcata caaatcgtat ttcaactgtt tacttaaata
     1261 atataattgc ttcaaagtat ctaacggtaa cttttcaaat ttttcaaaag ctaatatcat
     1321 caatttagca gtagtagcgg catcttcgtc agctcgatgg gcatttgcta aggtaatacc
     1381 atgtgcctct gctaattcac ttaattgata gcttttatct gtaggaaaag ctattttaaa
     1441 gatttctagt gtatctataa cttttttggg acgatattga atattacaat ctttaaatgc
     1501 ctttttaata aaattcaaat caaaatctac attatgagct acaaaaatgc aatcttttat
     1561 cttatcgtag atttcttgtg caacttgatt aaaatatggc gcttgttgta gcatattttc
     1621 ttcaatggat gttaacgctt gaatgaacgg cggaatctct aaatttgttc taatcataga
     1681 atgatatgta tcaataattt ggttattgcg cacaaacgtt ataccaattt gaatgatatc
     1741 gtcaaaatct aattggttgc ctgttgtttc caaatccaca acggcatagg ttgccatacc
     1801 catagctatc tctccttgct ttagtgttaa aaatctatat ctgcactaat taaacggtgt
     1861 gattcacccg cttcatctct aacaattaga tagccatcgt aatctaaatc aattgcttgt
     1921 cctttaaact gtttatcatt ttctgtaaat agcaacgttc tattccaaat attagaagct
     1981 gcagtatatt cttcacgaat ttcagaaaaa ggtaacgtta aaaattgatt atatcttttt
     2041 tcaatttctt gaagtaatat ctctaaaaat tgatatctat ctaatttatt tttatcatgt
     2101 aattgtatac ttgttgctct atgtctaata ctttcatcaa agttttctag ttgttgcgtc
     2161 aaattaatac ctataccaca tattattgct tctataccat cattattagc aaccatttca
     2221 gttaagaaac cacacacttt accattatca atatatatat cattcggcca tttcactttg
     2281 acttcatctt gactaaaatg ttgaatcgca tctcttatcc ctaatgcaat aaataaatta
     2341 aatttagata tcattgagaa tgcaacgtta ggtcttaaca cgacagacat ccaaagtcct
     2401 tgcccttttg aagaactcca atgtctatta aatcgcccac gacctttcgt ttgttcatca
     2461 ctcaagataa aaaatgaaga ttgatttcca acaagtgact ttttcgcagc aagttgtgta
     2521 gaatctattg aatcgtatac ttcactaaaa tcaaacaaag cagaactttt tgtatattgg
     2581 tctattatac cttgatacca aatatctggg agctgttgta ataaatgccc tttatgattt
     2641 actgaatcta ttttacatcc ctctaacttt aattggtcaa tcactttttt tactgcagtg
     2701 cgtgaaatat taagtgattc cgcaatgctt tgtccagata tataattcgg tttattttta
     2761 tagagtaatt gaagtacatc ttgactatat tttgacatga ttatccaccc atttcaaaat
     2821 ttcagtttct tcgttgctta ctttacctgt tacaatcgct atctcaattt gtcttagcac
     2881 atcttttaac cacggaccac ttttggcatt taaatgtgcc ataagtacac cgccattaac
     2941 catcatgtct tttctattat gcataggtaa acgatgtaat gtttcatcaa tcgtttgaag
     3001 gttaacgatt aatggttcat gtccttgaat atcatttgcc tttaacacgt ctgctgcaac
     3061 cattacattt ttaatgagat gcgtatcata atcataaaca aacattttta attgttcttt
     3121 tgtaataata cttggtaatg cattcataat ttgaatatat tgattgatat cttttacttg
     3181 tcggttactt agctttaaag gcttcaatga gtaattaata tcaaatttaa ctgatactat
     3241 agcaatcaac aattctaaat caattgcttc agttacatta atttgattca tatcaagatg
     3301 ttcgaaatac ggcatataat taaatgcttt cagcgatttt aaatgattaa aactcttttc
     3361 aacattaata cctcgcatta atttagttag ttcaatcact atacgctcaa ttgataaaaa
     3421 tttaatatct gccatttgta tacgcatcgc ttcgaatgtt tccgttgcaa tatcaaatga
     3481 taattgtgac tggaacctta aacatcgaat catacgtaaa gcatcttctt ggaatcgttc
     3541 ctcagctata cctacagttc ttattattcg attattaata tcttgttgac catcaaaata
     3601 atcatacaat ttgtatgctg tatccattgc tatcgcattc atcgtgaaat ctcgtcgttg
     3661 caaatcttcg tataaatcac gaacaaatgt aacaccactt ggtctacggt gatcgacata
     3721 atcttcttca gcccggaatg ttgtcacttc ataattttca tcattaaaaa ctacatttat
     3781 cgtgccatgt tctttaccta caggtatcgt atgactaaag atagattcta tttcatccgg
     3841 cgttgcactt gttgtgatat ctatatcatg aatatttctt cccatgacat aatctcttac
     3901 agagccacct acataatatg cttcaaaacc attgtcttga atttgttcta atataggcct
     3961 tgcctgttca aataatgatt tatccatatt attactcgcc tttacttttg ttatgctcat
     4021 ttagcatttt ttgataataa tactcatatt gatctgtaat aagttctgat ccaaaacgtt
     4081 cagcaatatc tgctagcatg tttttctgaa gtttgttgta taacacctta tcttcaagta
     4141 atcggatagc atagtcactc gctgaatcac aatcacccac atctacgaca aatccagttt
     4201 caccatgttt aataacctct ttaattccac cggcatttga accaattgga acgacgcctg
     4261 ttttcatagc ctcaagtaaa gttagtccaa agctttcttt ttcacttaat aataatacta
     4321 agtcagataa ttggtaaaat tcacttacgc aatcttgttt ccctaaaaat aaaacatcct
     4381 cttctacgtt taactctttc gtcaattgac gcattggcac taattcagga ccatctccaa
     4441 gtaaaattaa tttactaggt atcttttcac gtacttttgc aaatgtttct ataatagtat
     4501 ctatgcgttt tacttgtcta aaattcgata catgtattaa cactttttca tctggtgcta
     4561 taccaaattg tgattttaat gctgtgttat gtttagttgg aaactcattt tcacgtacaa
     4621 aattataaat cggtataatt tctttgttag tttcaataat ttcatgtgtt tcttgtgcta
     4681 aagatttact cacacttgtc acaatatcac ttttttcaat gccaaattta attgcacctt
     4741 ggagtgaatg atcatagccc aaaacagtaa tatcagtacc gtgtagcgtt gtcataattt
     4801 ttatatcttt acctgacatc tcacgagcta aaatcccaca aattgcatga ggtacagcat
     4861 agtgcatatg caacaaatca agatcatatt ctttaataac ttcagcgatt ttagtactta
     4921 acgtaatatc atacggtgga tactgaaata ctgcatattg attcacttca acttgatgaa
     4981 aaatcatatt cggtaatggt tttcttattc taaacgggat atttgaagtg ataaaatgta
     5041 cttcgtgacc tcgctctgct aatttaattc ctaattctgt ggcaataatt ccagaaccac
     5101 ccatggacgg gtaacatgtt atacctatct tcattcgctt gcccatcctt tctttctatt
     5161 tctctctatt attctcgatg cgtagataat tgtttaaatt taagtttata gtaatgttga
     5221 gtttataatt tcatatatct aaaaacaggt gttgtatata taatcattca tctagttata
     5281 cttactttaa aaataatata atttcatgcg atgcaattca ttgatggatg tttttaatct
     5341 taatcaaatc caaataaagc atatattttt aaattcactt tctttcgaat cgatttttat
     5401 ctcttgtatt aaacttttcc attgtttcat taaagctctc tgtcatatct attcccattg
     5461 aattcgctaa acataacaac acaaataaat tatcacctaa ttctgcttta atcgtatttg
     5521 cttcctctga atctttcttc tttttttcac cataggtatg atttatttca cgtgcaagtt
     5581 cgcccacttc ttcagtcaat ctagctaagt tagctaatgg tgaaaaatat cctgttttaa
     5641 attgtccaat atattcatca acttcacgtt gcatttctac cattgatttc atttctacgt
     5701 tctccttata ttgcatttct aatatagtat atatcaattt gaagtctcat gcatgtttag
     5761 tgcaatctac tgactctagc taaactagat gacaagttgt ttattttaga agtttaataa
     5821 ttcagtttat ataaatgtaa tgcattccta actaaattaa atcaattgaa attgggatta
     5881 taactttatg atacgtacca ctacaataaa ataatatagt gaataatcta ccattagaaa
     5941 aataagcaca aaaaaactag caaccacaca aaaatgtgat tagctagtta ataagtgtct
     6001 aatttaagtt aattgttaat ctataagatt aatcacttga acgcgcaatc aaaataatac
     6061 gtacaagctc tgctacagcg actgcagttg ctgcaacata agtcattgct gctgcagata
     6121 atactttacg cgcatgcttg tattcttttt catttacaat gttcaatgcc gtaatttgtt
     6181 tcatcgctct tgaactcgca tcaaactcaa ctggtaacgt aacaattgag aataataccg
     6241 ctaatgacat taaaccagca ccaatccata aagcagttga accaaatgca ctacctatcg
     6301 ctgttaagat aatacctaac atgatgatca tataacttaa tgaactccct aggtttgcaa
     6361 caggtactaa tgctgctctg aatcttaaga accaatatcc ttggtgatct tgaatggcat
     6421 gaccaacttc gtgggctgca attgcagttc cagcaactga tggtctgtca tagtttgcag
     6481 gagatagtga aacaactttc tttttaggat cgtaatgatc tgttaagaat ccttcacctt
     6541 taacaacttc gacatcataa ataccgtttg catgtaaaat ttctaatgca acttcacgac
     6601 ccgttttacc actagttgat ctaacttgtg aatatttctc atagttagat ttaactttgt
     6661 gttgtgccca taaaggaagc accattaata ttacgaaata aattatcata gtaaaaattg
     6721 aagacaataa actcactctc ctttataaat attttactgt catttgccgt ttttatcaaa
     6781 tcatttacac tttaataatt tgtttaattc aatataaagc aaaagtccaa aaacacttag
     6841 acaacatgat aatacaccaa tttgccacac atgtgtagtt ataaaatcat aatatggaaa
     6901 ttgaaggtga aaatagtcaa tataatcatt caaaaacacc caaatcatcg ctacactgat
     6961 tccaatcata gaacgtttaa acctaggata gaagtaaatt gcctgaacag ccattatact
     7021 gtgggaaaac attaatacca aaccatttac tgtaatatca ccttgttcaa taataaataa
     7081 tatattcatt ataactgccc aaatcccata tttgaataat gttacaaatg ccagtgcatc
     7141 gataatacta ttttgttttt gaattaatat caatgagata gaaataacta agtataatat
     7201 tgcagttggg ctatctggaa caaaaatctt aaaatgccag ggcgtatgac ttaattgttc
     7261 accataccat atataaccat aaatcatccc taatatatta caaatgagta gcatcattaa
     7321 ccaagaacgt tgataaagtg tatattgcca aaatgcttta attgtcatct gctaagtcct
     7381 caaattgatt atgtttattt actagcttga gtgtatttaa aatttgcgtt agttgataaa
     7441 aacgttgctt ttcattcatc tgtaaactta aatcaatatt gtgtaacaag taatctatta
     7501 ataacgcatg tttatgccga tctatagcca tactatttaa gtcatgaaga taagtttgat
     7561 aactgggcga ttccgttagt tgagcaacta ttaaatcatc tagcctttgc tcacgctttg
     7621 acacgtttgc gaagtgaatt tgaatatcaa aagcacagtt atgattagcg atataatcaa
     7681 atatttcatt tgtattcatt aactttatat tacgcttagt aaattgaatt gcagaagcgt
     7741 gacttcccac ttctgcaatt tctaatgttt catgatgatt aatttttgta tctacaaaat
     7801 gaatgtttgc caatttcgcc tcattcactt ttatatagtt aagcacccaa actgcaatac
     7861 gcgacttaaa tcgatattga aaaagtaaat attcaataaa actttcttta atttgattga
     7921 atgtctctga catcaaatac cccattttaa gattgcaatc ttgataattc gtcatgccaa
     7981 ttttcgttac ttggctctag ttccaacaat tgatttaaaa tagtaattgc ttgttccttt
     8041 tgaccaattt caattaaata gaaataataa tcactcataa aatcaatatt tgttttcatc
     8101 gttggatatg ctaattcaaa gaaatgttga gcttctttat ctcgctcttc ttgaccatag
     8161 gcgaacgcta aatgccacat gaatgtagga tccaaatctt cttcatctac atatgttaat
     8221 aattcaatga ttgcttcata atcttcttca ttacgatata aatcgcttaa aatcaataaa
     8281 ggttcttggt aagcattatc aacctctaat gcttgtttta acaataatac accttcatta
     8341 gcatcgccgt gttctatttc caaacatcca gttgtataca ttaactcttt ataaaattga
     8401 cttagtcgta atccttcttt acccgtttca atggcatctg gataattttt ttcattttca
     8461 tataatgatt gtaaatacaa gtagccttga atataatcag gatctttaga aagtaatgta
     8521 gtcattattt taattgcttc ttgagtgatg tcatttttat cgtaagaaat ggcttttttg
     8581 agataatctt ctgaagtcat ttcatcttca ttaatttcat cgtataagcg aatcgcatca
     8641 ctatagttac cactttgtaa actacaatct gccatacgag agaataagtt tacaccatta
     8701 acttgatatt caccagtttc taaaacggtt tcgtattcag aggtagcacg taaatattga
     8761 ccatcataat ataacatttc agccaatgca aaatggatta ttggatcatt tggctctagt
     8821 tcaagtgctt cttgtaattt atcaatagca acttccatca tattaatttg ttgatataaa
     8881 tctgcttcta acatcaactt ttcaggtgat ggttcaacat aacttaaata ttctaacgct
     8941 tcgtcagttt gattttcaga cattaaacct tcaataaaat aaatcagcaa ttcactttcg
     9001 tctggatatt tgtgatataa cacgcggaat acttccaaac cttgtggcat taatccaaaa
     9061 ttgtaaagtg tctctcctag aataaataat gcgtcatcgt tgtcagtagt tattgcttca
     9121 ttaacacgag agtctaaatt ttctagtttt tgtagattga tatcgtctat taatttatag
     9181 atatcttcca ttttatatta tccctcattt tctaaaagct ttagttttgg taaaaatcct
     9241 ggaaatgata catttacagc atcaaattgt ttgattttga caggctcgct tgaaagtaga
     9301 gaagcaactg caagcatcat tcctattcga tgatcagtta aactatcaac tgttgcattt
     9361 gttttaaatt ctgacggatg aataatcaat ccatcattag ttggttgtaa ttcaaaccct
     9421 aacaagttta acatatcagc cgttgtatca attctatttg tttcttttac ttttaattcc
     9481 tcggcatctt taattgtact cgtgccaact gcttgtgtac aaagtaatgc tattacaggc
     9541 agttcatcaa ttgcttttgg aactaattct ccttcgattg ttattggttg aagcattggt
     9601 gtgtattgaa tacgaataga agcagtaggt tcagcaccag ttgtttgatt gaaaagttgg
     9661 atattaccgc ccattttttc aacaatatca ataatacctg aacgtgttgg attgattcca
     9721 acattatgaa ttgttacatc acttcctggt gtgataagtg ctgcaacaat aaagaacgct
     9781 gcagatgaaa tatcgccagg aacatgaaaa tctgcaggtt taatgtatcg aattgcttca
     9841 ggggttgtat taattgataa cccttctgct tcaattggaa tattaaaatg tttgaacatc
     9901 gtctcagtat gatttcgact tacatctaat tctttaatga tggtcggttc cttagaaaac
     9961 aaacttgcaa ataaaatggc actttttact tgtgcacttg caacttccat ttgataattt
    10021 atacctttta tgacagatgg cttaataatt aatggtgtat aattatcttc aataccttca
    10081 atattcgcat ccataagttt caatggtctc aagacacgat ccattggcct tttaccaatt
    10141 gaaacatcgc cagacaaaac actttcaata cctaaaccac ttaacaaacc tgccaataat
    10201 cgtgttgtcg taccagaatt acctgtatac aatacttgat gtggcgtgtt aaaagattga
    10261 tatcctgggg aagtcacaac taatttttca tcatcttctt tgatttctac acctaacagt
    10321 cggaaaatgt ccatcgtacg acgacaatct tcgccaagta gtggcttata tatagtagat
    10381 acaccttcag ctagcgacgc caacatgatt gcacggtgtg tcattgactt atcgcccggc
    10441 acttctattt cgccctttaa cggacctgaa atatcaatga tttgttcatt taccatttca
    10501 ttcacctact taaaatatgt ttttaattgt tcacatgcat gttgtaatgt tagttgatca
    10561 acatgttgta caacgatatc tccaaattgt ctaatcaaga ccatttgtac accttgctta
    10621 tcattctttt tatcacttag catatattgg tataacgttt caaaatccaa gtcagttatc
    10681 atgtctaaag gatagccgag ttgtattaaa tattgaatat aatgattaat atcatgctta
    10741 gaatcaaaca aagcattcgc aactataaat tgatagataa tgccaaccat cactgcatga
    10801 ccatgaggta ttttatgata gtattcaaca gcatgaccaa atgtatgacc taaatttaaa
    10861 aatttacgta caccttgttc tttttcatct gcaataacaa tatccagctt cgtttcaata
    10921 cctttagcaa tatatttatc cataccattt aatgactgta atatctctct atctttaaag
    10981 tgctgttcga tatcttgcgt cgctgattca ccattcaata acgcatgctt ataaacttct
    11041 gcatagccac ttaatatttg ctcaaatggt aacgtcttta aaaagtctaa atcataaatc
    11101 acagcagttg gacgataaaa tgcaccgata aggtttttac cttgctttga gttaataccc
    11161 actttaccgc caacactaga atcatgcgct agtatagtcg ttggcacttg tataaagtga
    11221 acgcctcgta aaagtgtcgc cgcaataaac ccagcaaaat caccagttgc accaccacca
    11281 acagcaataa ttgctgtatt acgagttaca tgatgggata aaatatactc taatgtttct
    11341 tgatattgct caaatgtttt cgtcttttca ccagctggaa taataacttt atgtacattt
    11401 tcatatgata aaatatcatc aaatttatca gcaaaatatt gatttacatg ctcgtcaatt
    11461 aatataaaac tttgatcaaa ctgatcaata tacgtgctaa tatggtcaat tgcaccgtgt
    11521 tcaacatata ttggataatt atttgaaggg tatgttgttt gtaatttcat gattacacct
    11581 caattgttct tgttgttaaa actcaatatt taattgtctg cgctcaataa tttgttgttt
    11641 aagttgctca atatgatttg attggaattc ttccaataat gcttttgcta tttcaaatgc
    11701 tacgacatgt tcgcagacga tacttgctgc aggaacagca caactatcag aacgttcaat
    11761 tgttgcttta aagtcttctt tagtattaat gtctactgaa tttaatggtt tatataacgt
    11821 tggaattggt ttcattacac cattaacgat aattggcatt ccatttgaca taccgccttc
    11881 taaaccacct aagtgattag atccacgata ataaccaatt tcactattat atagaatttc
    11941 atcttgaatc tcactacctg gcttttcagc tgctttaaat ccttcaccaa agcttacacc
    12001 tttaaaagca tttatgctga caacaccttg tgcaatctta ccatctaact tacgatcata
    12061 atgcacataa ctacctacac caacaggcat attttcaact acaacttgaa cgacaccgcc
    12121 aattgaatct ccttcatttt tagcttcgtc aattttatct cgcattgctt gtgcgatact
    12181 gtcatcaatt acacgaacat cattacgatc aagatttgct ttaaatgttt ctgaatcata
    12241 aaaatcttta tctttaattc cacctatttc aacaacacga ctgtatatat cgatatctaa
    12301 ctgttgtaat aacactttac ataaggcacc gactgcaact cgagctgctg tttctctagc
    12361 agatgatcgc tctagcacat ttcgtaaatc acgatgatta tatttcatac ctccaaccaa
    12421 atctgcatga ccaggtcttg gttttgtaat agtacgtttc atattttcac gttcttcttc
    12481 acttattgga gctgctccca taatttttct ccaatgcgta aagtcatcat tggttacaac
    12541 catagtaatt ggactaccta atgtataacc atttctaacg cctgatacta tttctactgt
    12601 atctttctca atttgcatac gacgtccacg tccgtaaccg ccttgacgct taaacatttc
    12661 tttattaata tcctcaacct taatttctat atttgcaggt acaccttcaa caataactgt
    12721 taattgaggt ccatgtgatt ctcctgatgt taggtatctc atacacactc gctcctttca
    12781 tacttccacg ataacattat aatatgttgt taatcttatc atattcattg tgcattcata
    12841 aatgtttgtt ttcaagctca attaagaaaa tggatgacat ctcattttaa atgtcagaat
    12901 tatcttaata ttaaacgaac taaaattaat tataatatat tcgtctgtag aaaagtatca
    12961 tgaatttcta aacagttgca tcatataatc attaattaaa aatgattagc cgttttaata
    13021 taatcgtatc gcaactttag caaataatat actgactaca tttcttaata caaaagaacg
    13081 aactgttaat
//
LOCUS       DAKTGS010000079        12850 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.079, whole genome shotgun sequence.
ACCESSION   DAKTGS010000079 DAKTGS010000000
VERSION     DAKTGS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.079"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            963..2300
                     /locus_tag="O0286_001778"
     CDS             963..2300
                     /locus_tag="O0286_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCY0170155.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            2302..2919
                     /locus_tag="O0286_001779"
     CDS             2302..2919
                     /locus_tag="O0286_001779"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCY0170156.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2947..3276
                     /locus_tag="O0286_001780"
     CDS             2947..3276
                     /locus_tag="O0286_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCY0170157.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            3490..4416
                     /locus_tag="O0286_001781"
     CDS             3490..4416
                     /locus_tag="O0286_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCY0170158.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            4417..5220
                     /locus_tag="O0286_001782"
     CDS             4417..5220
                     /locus_tag="O0286_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCY0170159.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            5237..5584
                     /gene="yabA"
                     /locus_tag="O0286_001783"
     CDS             5237..5584
                     /gene="yabA"
                     /locus_tag="O0286_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCY0170160.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5858..6583
                     /locus_tag="O0286_001784"
     CDS             5858..6583
                     /locus_tag="O0286_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCY0170161.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            6576..6824
                     /locus_tag="O0286_001785"
     CDS             6576..6824
                     /locus_tag="O0286_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCY0170162.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6826..7665
                     /gene="rsmI"
                     /locus_tag="O0286_001786"
     CDS             6826..7665
                     /gene="rsmI"
                     /locus_tag="O0286_001786"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCY0170163.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7950..9923
                     /gene="metG"
                     /locus_tag="O0286_001787"
     CDS             7950..9923
                     /gene="metG"
                     /locus_tag="O0286_001787"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCY0170164.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9954..10727
                     /locus_tag="O0286_001788"
     CDS             9954..10727
                     /locus_tag="O0286_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCY0170165.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10894..11430
                     /gene="rnmV"
                     /locus_tag="O0286_001789"
     CDS             10894..11430
                     /gene="rnmV"
                     /locus_tag="O0286_001789"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCY0170166.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            11441..12334
                     /gene="rsmA"
                     /locus_tag="O0286_001790"
     CDS             11441..12334
                     /gene="rsmA"
                     /locus_tag="O0286_001790"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCY0170167.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            12434..12697
                     /locus_tag="O0286_001791"
     CDS             12434..12697
                     /locus_tag="O0286_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCY0170168.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
BASE COUNT         4687 a         1719 c         2571 g         3873 t
ORIGIN      
        1 ccatgccgaa cacagaagtt aagctcctta gcgtcgatgg tagtcgaact tacgttccgc
       61 tagagtagaa cgttgccagg caaaaaatgg atgcgatgag ccgcattgag accgcaaggt
      121 ctcttttttt atgtctaaaa cgtcaaaata aaaagcaaac acaaagaaaa atggcttggc
      181 gaagtgaaaa cgtttgaatc tgacgaaacg agaaaagagc gcaacgagtt tagtagagct
      241 aaatgagtaa gcgagagccg aagaagagga aagaagcaag cgattgtcac aagtcaagaa
      301 aggtctttag cgacgatggt agccaactta cgttccgcta gagtagaacg ttgccaggca
      361 agttgagacc gtgaggtctc tttttttatg tctaaaacgt caaaataaaa agtaaacaca
      421 aagaaaaatg gcttggcgaa gtgaaaacgt ttgaatctga cgaaacgaga aaagagcgca
      481 acgagtttag tagagctaaa tgagtaagcg agagccgaag gagaggaaag aagcaagcga
      541 ttgtcacaag tcaagaaagg ttcttagcga ggatggtagc taacttacgt tccgctagag
      601 tagaacgttg ctagacaaga aatgaatgcg atgagccgca ttgagtgtga aattgatatt
      661 ttaataatgt gcacttttga ttatttaaat ctgtgactag aagtataagt gaagacattc
      721 agaagtatta taaaaagtga acagcagtaa gatagttttt aatcataaat catcttactg
      781 ctgtttttag attttatgtc taatatcttt taaatcgaag tacaaaaagg aaattaatta
      841 ttatacaata gacaagctat tgcataagta acactaactt ttgtcaaaga agtgttacta
      901 tataattaat acttttgaaa gtaactaatt ccaaaacagt gtaaataaag gaagcgtata
      961 tcatgaagca acctatttta aataaattag aaagtttaaa tcaagaagaa gcgatttctt
     1021 tgcatgttcc gggtcataaa aatatgacta tcggtcattt atctcaatta tcaatgacaa
     1081 tggataaaac tgaaatacct ggattagatg atttacatca tcctgaagaa gtcattttgg
     1141 aaagtatgaa gcaggtggag aaacattcag attatgatgc ttatttctta gtgaatggca
     1201 ccacttcagg aatattatct gtcatccagt ctttttcaca gaaaaaaggc gatatcttaa
     1261 tggcaagaaa tgtacataaa tctgtgttac atgcgctcga tattagccaa caagaagggc
     1321 attttattga aacgcatcaa agtccgttaa cgaatcatta taataaagtt aatttaagcc
     1381 gtttgaataa tgacggtcac aaacttgctg tgttgactta tcctaactat tacggtgaaa
     1441 catttaatgt agaagaggtt atcaaatttt tgcaccaatt aaatattcct gtactcattg
     1501 acgaagcaca cggcgcgcac tttggattgc aaggatttcc agattctaca ttaaattatc
     1561 aagctgacta tgttgttcaa tcttttcata aaacgttacc agctttaacg atgggctcgg
     1621 tactttatat tcataaaaat gcaccttata gagaaactat tatagaatat ctaagctact
     1681 tccaaacatc tagtccttcg tatttgatta tggctagttt agagtcagct gccgagttct
     1741 ataaaacata tgatagtacc gtgttttttg ataagagagc gcaattaatc gaatgtttgg
     1801 agaagaaggg ttttgaaatg cttcaagttg atgatccgtt gaagttgctg ataaaatatg
     1861 aaggttttac aggtcatgat attcaaaatt ggtttatgaa tgcacatatc tatttagaat
     1921 tagcggacga ctatcaagca ttagcgatat tgccgttatg gcatcatgat gatacgtatt
     1981 tatttgattc gcttttacgt aaaattgaag atatgatttt accgaaaaaa tcagtttcta
     2041 aagttaaaca aacacaactt ttaacaactg aaggtaacta taaaccaaaa cgctttgaat
     2101 atgttacttg gtgtgatttg aaaaaggcaa aaggtaaagt tctggcgcga catattgtcc
     2161 cgtatccgcc agggattcct attattttca aaggagaaac aataactgaa aatatgatag
     2221 aattggtaaa tgaatatctg gaaactggaa tgatagttga aggaattaaa aataataaaa
     2281 ttttagttga ggatgaataa aatgtcagct tttataactt ttgagggccc agaaggctct
     2341 ggaaaaacaa ctgtaattaa tgaagtttac catagattag taaaagatta tgatgtcatt
     2401 atgactagag aaccaggtgg tgttcctact ggtgaagaaa tacgtaaaat tgtattagaa
     2461 ggcaatgata tggacattag aactgaagca atgttatttg ctgcatctag aagagaacat
     2521 cttgtattaa aggtcatacc agctttaaaa gaaggtaagg ttgtgttgtg tgatcgctat
     2581 atcgatagtt cattagctta tcaaggttat gctagaggga ttggcgttga agaagtaaga
     2641 gcattaaacg aatttgcaat aaatggatta tatccagact tgacgattta tttaaatgtt
     2701 agtgctgaag taggtcgcga acgtattatt aaaaattcaa gagatcaaaa tagattagat
     2761 caagaagatt taaagtttca cgaaaaagta attgaaggtt accaagaaat cattcataat
     2821 gaatcacaac ggttcaaaag cgttaatgca gatcaacctc ttgaaaatgt tgttgaagac
     2881 acgtatcaaa ctatcatcaa atatttagaa aagatatgat ataattgtta gaagaggtgt
     2941 tataaaatga aaatgattat agcgatcgta caagatcaag atagtcagga acttgcagat
     3001 caacttgtta aaaataactt tagagcaaca aaattggcaa caacaggtgg gtttttaaga
     3061 gcgggtaata caacattctt atgtggtgtc aatgatgacc gtgtagatga aatattgtct
     3121 gtgattaatc aaacgtgtgg taatagagaa cagttggttt cacctattac acctatggga
     3181 ggcagtgcgg attcgtacat tccatatcca gttgaagttg aagttggcgg tgctactgta
     3241 tttgttatgc cagttgatgc attccatcaa ttttaattct ataatacaat catcaattag
     3301 agatacttaa aatagtgtat taataagtta ttaacaattt tgggttgctt gactgcgact
     3361 agttcagatg ccaatagatt tgatttttgt ggttctaaaa ataatcacaa atcatggtcg
     3421 ctattgttgc agtaattagt tgctctttgg caaccttttt atataaaagc aaaagggagt
     3481 ttgtaatgaa tggatgaaca gcaacaattg acgaatgcat atcattcaaa taaattatcg
     3541 catgcctatt tatttgaagg tgatgatgca caaacgatga aacaagttgc gattaatttt
     3601 gcaaagctta ttttatgtca aacagatagt caatgtgaaa caaaggttag tacatataat
     3661 catccagact ttatgtatat atcaacaact gagaatgcaa ttaagaaaga acaagttgaa
     3721 caacttgtgc gtcatatgaa tcaacttcct atagaaagca caaataaagt gtacatcatt
     3781 gaagactttg aaaagttaac tgttcaaggg gaaaacagta tcttgaaatt tcttgaagaa
     3841 ccaccggaca atacgattgc tattttattg tctacaaaac ctgagcaaat tttagacaca
     3901 atccattcaa gatgtcagca tgtatatttc aagcctattg ataaagaaaa gtttataaat
     3961 agattagttg aacaaaacat gtctaagcca gtagctgaaa tgattagtac ttatactacg
     4021 caaatagata atgcaatggc tttaaatgaa gaatttgatt tattagcatt aaggaaatca
     4081 gttatacgtt ggtgtgaatt gttgcttact aataagccaa tggcacttat aggtattatt
     4141 gatttattga aacaggctaa aaataaaaaa ctgcaatctt taactattgc agctgtgaat
     4201 ggtttcttcg aagatatcat acatacaaag gtaaatgtag aggataaaca aatatatagt
     4261 gatttaaaaa atgatattga tcaatatgcg caaaagttgt cgtttaatca attaattttg
     4321 atgtttgatc aactgacgga agcacataag aaattgaatc aaaatgtaaa tccaacgctt
     4381 gtatttgaac aaatcgtaat taagggtgtg agttagatgc caaatgtaat aggtgttcag
     4441 tttcaaaaag cgggaaaatt agaatattat acacctaatg atatacaagt agatatagaa
     4501 gactgggtag ttgtcgaatc taaaagaggc atagagatag gtattgttaa aaatccatta
     4561 atggatattg ctgaagagga tgttgtgtta cctcttaaaa atattattcg cattgctgat
     4621 gacaaagata ttgataaatt taattgtaat gaacgagatg ctgaaaatgc attaatacta
     4681 tgtaaagaca ttgtaagaga acaaggtttg gacatgcgtt tagtcaattg cgaatataca
     4741 ttagataaat cgaaagttat ttttaatttt acggcggatg atcgtattga ttttagaaaa
     4801 ttagtaaaaa tattagcgca acatttaaaa acacgtatcg agttgagaca aattggtgta
     4861 agggatgaag ccaaattgct tggcggtatc ggaccttgtg gtaggtcgtt atgttgttct
     4921 acatttttag gggattttga accagtatcg attaagatgg ctaaggatca aaatttatca
     4981 ttaaatccaa ctaaaatttc tggtgcatgt ggtcgtttga tgtgttgttt aaaatatgaa
     5041 aatgactatt atgaggaagt acgtgcacaa ttacctgata ttggtgaagc aattgaaacg
     5101 cctgatggta acgggaaagt agttgcttta aatatattag acatttctat gcaggtgaag
     5161 cttgagggac atgaacagcc acttgaatat aaattagaag aaatagaaac tatgcattaa
     5221 ggaggcatta ttacatttgg atcgcaatga aatatttgaa aaaataatgc gtttagaaat
     5281 gaatgtcaat caactttcaa aggaaacttc agaattaaag gcacttgcag ttgaattagt
     5341 agaagaaaat gtagcgcttc aacttgaaaa tgataatttg aaaaaggtgt tgggcaatga
     5401 tgaaccaact actattgata ctgcgaattc aaaaccagca aaagctgtga aaaagccatt
     5461 accaagtaaa gataatttgg ctatattgta tggagaagga tttcatattt gtaaaggcga
     5521 attatttgga aaacatcgac atggtgaaga ttgtctgttc tgtttagaag ttttaagtga
     5581 ttaatcaagc acactcaaat agtgttataa ttataaatga atatggtttg gataagtctg
     5641 agacaatgca tgtttcaggc tttaattgtg tataaagttt tggtgattgc ataagagatg
     5701 gcggtactaa atgttattat taagtgtgca cgcagtatca ttagttataa aatgtagctg
     5761 ttaaaagtca aaaatacatc gaatgtagtt aggcatataa tataaaaaga gttttcaatt
     5821 actcaataga aaaaggttgt cttcatagga gttaaaaatg ttaaaagaga atgaacgatt
     5881 tgatcaacta atcaaagaag attttagtat tattcaaaat gatgatgttt tttcattttc
     5941 aacggatgct ttgttgttag ggcattttac aaaacctaga acaaaagata ttgtgttgga
     6001 cttatgttca ggcaatgggg tgataccctt gttattgttt gcgaaacatc cacgacatat
     6061 agaaggtgtt gagattcaaa aaacacttgt cgatatggcg cgacgcacat ttcaattcaa
     6121 tgatgttgat gaatatttaa caatgcatca catggatttg aaaaacgtta ctaaagtatt
     6181 taaaccttca caatatactt tagtaacgtg taatccgcct tattttaaag agaatcagca
     6241 acaccaacat caaaaagaag cacataagat agcgagacat gagattatgt gtacacttga
     6301 agattgcatg attgcagccc gtcatttatt aaaagaaggt ggcaggctaa acatggtaca
     6361 tcgtgcagag agactaatgg atgtcttgtt tgaaatgaga aaagtgaata ttgaacctaa
     6421 gaaagtcgtt tttatatata gtaaagtagg gaaatcagca caaacgatag tagtagaagg
     6481 tcgaaaaggt ggaaatcaag gtttagaaat catgccccca ttttatattt ataatgaaga
     6541 tggtaattat agcgaagaaa tgaaggaagt atattatgga tagtcatttt gtatatattg
     6601 taaaatgtag tgatggaagt ttatatacag gatacgctaa agacgttaat gcacgtgttg
     6661 aaaaacataa ccgaggtcaa ggagccaaat atacgaaagt aagacgtccg gtgcatttag
     6721 tttatcaaga aatgtatgag acaaagtctg aagcattgaa gcgtgaatat gaaattaaaa
     6781 cttataccag acaaaagaaa ttgcgattaa ttaaggagcg atagtatggc tgtattatat
     6841 ttagtgggca caccaattgg taatttagca gatattactt atagagcagt tgatgtattg
     6901 aaacgtgttg atatgattgc ttgtgaagac actagagtaa ctagtaaact gtgtaatcat
     6961 tatgatattc caactccatt aaagtcatat cacgaacata acaaggataa gcagactgct
     7021 tttatcattg aacagttaga attaggtctt gacgttgcgc tcgtatctga tgctggattg
     7081 cccttaatta gtgatcctgg atacgaatta gtagtggcag ccagagaagc taatattaaa
     7141 gtagagaccg tgcctggacc taatgctggg ctgacggctt tgatggctag tggattacct
     7201 tcatatgtat atacattttt aggatttttg ccacgaaaag agaaagaaaa aagtgctgta
     7261 ttagagcaac gtatgcatga aaatagcaca ttaattatat acgaatcacc gcatcgtgtg
     7321 acagatacat taaaaacaat tgcaaagata gatgcaacac gacaagtatc actagggcgt
     7381 gaattaacta agaagttcga acaaattgta actgatgatg taacacaatt acaagcattg
     7441 attcagcaag gcgatgtacc attgaaaggc gaattcgtta tcttaattga aggtgctaaa
     7501 gcgaacaatg agatatcgtg gtttgatgat ttatctatca atgagcatgt tgatcattat
     7561 attcaaactt cacagatgaa accaaaacaa gctattaaaa aagttgctga agaacgacaa
     7621 cttaaaacga atgaagtata taatatttat catcaaataa gttaatcact ttatcgatta
     7681 tatgaaattt taaacgattt tataaacgca agctgtaatt ttaaatggta agttatcatt
     7741 ttgcattgat actgataaaa tgatgttgac tatgataaaa aaatgatgac atcgacgttt
     7801 tttaatgtaa aataaataca ttgaaagtaa taaatacctt aacattgaat aagatgaaaa
     7861 tgagatgacg agataaatgt tcgcgtccgt tgaaatgcat agaaatctta gatattattt
     7921 gaagtgagac attacgagga ggaacagtta tggctaaaga aacattttat ataacaaccc
     7981 caatatacta tcctagtggg aatttacata taggacatgc atattctaca gtggctggag
     8041 atgttattgc aagatataag agaatgcaag gatatgatgt tcgctatttg actggaacgg
     8101 atgaacacgg tcaaaaaatt caagaaaaag ctcaaaaatc tggtaagaca gaaattgaat
     8161 atttggatga gatgattgct ggaattaaac aattgtgggc taagcttgaa atttcaaatg
     8221 atgattttat cagaacaact gaagaacgtc ataaacatgt cgttgagcaa gtgtttgaac
     8281 gtttattaaa gcaaggtgat atctatttag gtgaatatga aggttggtat tctgttccgg
     8341 atgaaacata ctatacagag tcacaattag tagacccaca atacgaaaac ggtaaaatta
     8401 ttggtggcaa aagtccagat tctggacacg aagttgaact agttaaagaa gaaagttatt
     8461 tctttaatat tagtaaatat acagaccgtt tattagagtt ctatgaccaa aatccagatt
     8521 ttatacaacc accatcaaga aaaaatgaaa tgattaacaa cttcattaaa ccaggacttg
     8581 ctgatttagc tgtttctcgt acatcattta actggggtgt ccatgttccg tctaatccaa
     8641 aacatgttgt ttatgtttgg attgatgcgt tagttaacta tatttcagca ttaggctatt
     8701 tatcagatga tgagtcacta tttaacaaat actggccagc agatattcat ttaatggcta
     8761 aggaaattgt gcgattccac tcaattattt ggcctatttt attgatggca ttagacttac
     8821 cgttacctaa aaaagtcttt gcacatggtt ggattttgat gaaagatgga aaaatgagta
     8881 aatctaaagg taatgtcgta gaccctaata ttttaattga tcgctatggt ttagatgcta
     8941 cacgttatta tctaatgcgt gaattaccat ttggttcaga tggcgtattt acacctgaag
     9001 catttgttga gcgtacaaat ttcgatctag caaatgactt aggtaactta gtaaaccgta
     9061 cgatttctat ggttaataag tactttgatg gcgaattacc agcgtatcaa ggtccacttc
     9121 atgaattaga tgaagaaatg gaagctatgg ctttagaaac agtgaaaagc tacactgaaa
     9181 gcatggaaag tttgcaattt tctgtggcat tatctacggt atggaagttt attagtagaa
     9241 cgaataagta tattgacgaa acaacgcctt gggtattagc taaggacgat agccaaaaag
     9301 atatgttagg caatgtaatg gctcacttag ttgaaaatat tcgttatgca gctgtattat
     9361 tacgtccatt cttaacacat gcgccgaaag agatttttga acaattgaac attaacaatc
     9421 ctcaatttat ggaatttagt agtttagagc aatatggtgt gcttaatgag tcaattatgg
     9481 ttactgggca acctaaacct attttcccaa gattggatag cgaagcggaa attgcatata
     9541 tcaaagaatc aatgcaaccg cctgctacta aagaggaaaa agaagagatt cctagcaaac
     9601 ctcaaattga tattaaagac tttgataaag ttgaaattaa ggcagcaacg attattgatg
     9661 ctgaacatgt taagaagtca gataagcttt taaaaattca agtagactta gattctgaac
     9721 aaagacaaat tgtatcagga attgccaaat tctatacacc agatgatatt attggtaaaa
     9781 aagtagcagt tgttactaac ctgaaaccag ctaaattaat gggacaaaaa tctgaaggta
     9841 tgatattatc tgctgaaaaa gatggtgtat taaccttagt aagtttacca agtgcaattc
     9901 caaatggtgc agtgattaaa taactgtatt tttaaaaatt aggagagata attatgttaa
     9961 tcgatacaca tgtccattta aatgatgagc aatacgatga tgatttgagt gaagtgatta
    10021 cacgtgctag agaagcaggt gttgatcgta tgtttgtagt tggttttaac aaatcgacaa
    10081 ttgaacgcgc gatgaaatta atcgatgagt atgatttttt atatggcatt atcggttggc
    10141 atccagttga cgcaattgat tttacagaag aacacttgga atggattgaa tctttagctc
    10201 agcatccaaa agtgattggt attggtgaaa tgggattaga ttatcactgg gataaatctc
    10261 ctgcagatgt tcaaaaggaa gtttttagaa agcaaattgc tttagctaag cgtttgaagt
    10321 taccaattat cattcataac cgtgaagcaa ctcaagactg tatcgatatc ttattggagg
    10381 agcatgctga agaggtaggc gggattatgc atagctttag tggttctcca gaaattgcag
    10441 atattgtaac taataagctg aatttttata tttcattagg tggacctgtg acatttaaaa
    10501 atgctaaaca gcctaaagaa gttgctaagc atgtgtcaat ggagcgtttg ctagttgaaa
    10561 ccgatgcacc gtatctttcg ccacatccgt atagagggaa gcgaaatgaa ccggcgagag
    10621 taactttagt agctgaacaa attgctgaat taaaaggctt atcttatgaa gaagtgtgcg
    10681 aacaaacaac taaaaatgca gagaaattgt ttaatttaaa ttcataaagt taaaagtgag
    10741 aaagatcacc gccataaatg taaacgatgc tatattcgtt taatatgcta tggttctttc
    10801 tcactttttt aaattaaaat atcgtgcatg tggaatacgt gcgatagaga tggttagagc
    10861 tttgaaatta agaattgtag gaaggcgttt taaatgaaaa tcaatgagtt tatagttgta
    10921 gaaggacgag atgatactga gcgtgttaaa cgagctgttg aatgtgatac gattgaaacg
    10981 aatggtagtg ccatcaacga acaaacttta gaagtaatta gaaatgctca acaaagtcga
    11041 ggcgttattg tattaacaga tccagatttc ccaggagata aaattagaag tacaattact
    11101 gaacatgtca aaggtgttaa acatgcgtat attgatagag aaaaagctaa aaataaaaaa
    11161 gggaaaattg gtgttgaaca tgccgaatta attgatatta aagaagcgtt aatgcatgtt
    11221 agttcaccct ttgatgaagc ttatgaatca attgataaat ctgtgctaat agagttgggg
    11281 ttaattgttg ggaaagatgc aaggcgccgt agagaaattt taagtagaaa attgcgaatc
    11341 ggccattcca atggtaagca gttattgaaa aagttaaatg catttggtta taccgaagcg
    11401 gatgtaaggc aagctttaga agatgaatga ggaagtgaaa atgttggata ataaagatat
    11461 tgcaacacca tcaagaacgc gagcgttgtt agataaatat ggctttaatt ttaaaaaaag
    11521 tttaggacag aactttttga tagatgtgaa tatcattaat aatatcattg atgcaagtga
    11581 tattgatgca caaactgggg tgattgaaat tggtccaggc atggggtcat tgacagaaca
    11641 attggccaga catgctaaaa gagtattggc atttgaaatt gatcaacgtt taatacctgt
    11701 attaaatgat acactatcac cttatgataa tgtgacggtg attaatgaag atattttaaa
    11761 agcgaatatt aaagaagctg ttgaaaatca tttacaagat tgtgaaaaaa taatggttgt
    11821 tgcaaacctg ccgtactata ttacgacgcc aattttatta aatttgatgc aacaagatat
    11881 accaattgat ggctacgtgg tgatgatgca aaaagaagtg ggcgaacgct taaatgctga
    11941 agtaggttca aaagcatatg gttcgttatc aattgtcgta caatactata cagagactag
    12001 taaagtatta acggtaccta aatctgtatt tatgccacca cctaatgttg attcaatagt
    12061 tgtaaaactg atgcagagaa ctgaaccgtt agtaacagta gataacgagg aagcattctt
    12121 taagttagca aaagcagcat ttgcacaaag aagaaagaca attaacaata actatcaaaa
    12181 ttattttaaa gatggtaaac aacacaaaga agtgatttta caatggttgg aacaagcagg
    12241 tattgatcca agacgtcgcg gtgaaacgct atctattcaa gattttgcta aattgtatga
    12301 agaaaagaaa aaattccctc aattagaaaa ttaaatgatt gacaaagcaa agcactattg
    12361 ttaaaattta aattttgttt gacgaaaacg ttgcaaatat ggtattatgt aacttgtagc
    12421 gaggtggagc aatatgccaa aatcaatttt ggacatcaaa aattctattg attgtcatgt
    12481 aggaaatcgt attgtactga aagccaatgg aggccgtaag aaaacaataa aacgttctgg
    12541 aattttaaaa gaaacatatc cgtcagtttt cattgttgag ttagatcaag acaaacacaa
    12601 ctttgagaga gtatcttata catacactga tgtgttaact gaaaatgttc aagtttcatt
    12661 tgaagaggat aatcatcacg aatcaattgc acactaaata agacatatag agatgttaga
    12721 cgtttcttag tataagaagt aaatattatg ataattattt gagtgttggg cattatgttc
    12781 aatactcttt ttatttacaa aatgtttaac actgatgttt cgcttataga tttttcagta
    12841 aatggataat
//
LOCUS       DAKTGS010000080        12648 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.080, whole genome shotgun sequence.
ACCESSION   DAKTGS010000080 DAKTGS010000000
VERSION     DAKTGS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.080"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(112..627)
                     /locus_tag="O0286_001792"
     CDS             complement(112..627)
                     /locus_tag="O0286_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170169.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            818..1822
                     /locus_tag="O0286_001793"
     CDS             818..1822
                     /locus_tag="O0286_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0170170.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(1868..2269)
                     /locus_tag="O0286_001794"
     CDS             complement(1868..2269)
                     /locus_tag="O0286_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170171.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(2305..3339)
                     /locus_tag="O0286_001795"
     CDS             complement(2305..3339)
                     /locus_tag="O0286_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0170172.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(3350..3466)
                     /locus_tag="O0286_001796"
     CDS             complement(3350..3466)
                     /locus_tag="O0286_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170173.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(3571..4002)
                     /locus_tag="O0286_001797"
     CDS             complement(3571..4002)
                     /locus_tag="O0286_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCY0170174.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(4209..5486)
                     /locus_tag="O0286_001798"
     CDS             complement(4209..5486)
                     /locus_tag="O0286_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0170175.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            5647..6270
                     /locus_tag="O0286_001799"
     CDS             5647..6270
                     /locus_tag="O0286_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0170176.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            6316..7260
                     /locus_tag="O0286_001800"
     CDS             6316..7260
                     /locus_tag="O0286_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCY0170177.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(7346..8788)
                     /locus_tag="O0286_001801"
     CDS             complement(7346..8788)
                     /locus_tag="O0286_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCY0170178.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            8971..9354
                     /locus_tag="O0286_001802"
     CDS             8971..9354
                     /locus_tag="O0286_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCY0170179.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            10842..>12648
                     /gene="rsp"
                     /locus_tag="O0286_001803"
     CDS             10842..>12648
                     /gene="rsp"
                     /locus_tag="O0286_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCY0170180.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFL"
BASE COUNT         4299 a         2164 c         1803 g         4382 t
ORIGIN      
        1 tttctataaa tttaattatt ttactttaac aattcattac gcatttagca tttcaaggta
       61 tacacaatat ttattactat gatttcattt tatctgctgc aaaaacaatc attataactc
      121 tttttccata attaaatctg tatccgttac atcacctgtt tgaaaatgat gttcaccaac
      181 cactttaaat ccatgacgtt tataaaatgc ttgagcacga ggattatgct cccaaactcc
      241 tagccaaatt ttatgtttat tatgttcttg agcaattttt tcggccaatt ctatcaattg
      301 tgaacctctt ccgccacctt gaaagtcttt caaaaaatat atgcgctgca cttctaaata
      361 ggtctccccc atttcttcag tttgagcact attaatattc atctttatat aaccaacatt
      421 cgcaccatct tcttgataaa aataatgaaa tgaatctaca tggttaatct cttgtgtaaa
      481 tttctctaca gtataattgt ctttaaaaaa ttgatcaaaa tctttgtcat catagtaaga
      541 accaaacgtg tcataaaatg ttctagttgc taattcaact aattcactag cattttgttc
      601 tgaaatttct ttgattatcc cagccatata aatcctccaa taaacagtga tcgaatcaaa
      661 atattactta tgttattttt cagccaaaac tatttaaaaa tacattaaca caaatcaatt
      721 acaaattgta ttgattgtgt gtaacatcaa taaatgatac atttattcca gtaaaatggc
      781 cgtattttca aaagagaaaa agagaggatg tatcgttgtg atagaaacat ttaaagcgtt
      841 tgtaattgat aaagatgaga gtggtaaagt gacaccaact ttcaaacaat tatcgcctac
      901 tgatttacct aaaggagatg tgctgattaa agtacattac tctggtataa attataaaga
      961 tgctttagcg actcaagatc ataatgcagt cgtaaaatcg tatcctatga ttccaggaat
     1021 agatttagct ggaacaattg ttgaatccga agcaccaggc tttgaaaaag gagaacaagt
     1081 aattgtaacg agttatgacc taggtgtcag ccattatggc ggttttagtg aatatgcgcg
     1141 tgtaaaatca gaatggatta tcaagcttcc tgatacttta acattagaag aatcaatgat
     1201 atatggcaca gctggttata ctgccggttt agcaattgaa agacttgaaa aagttggaat
     1261 gaatattgaa gatggtcctg tactcgttcg cggtgcttca ggtggtgtcg gtactttagc
     1321 agtactcatg cttaatgaac ttggttataa agttatcgca agtacaggta aacaagatgt
     1381 tagcgatcaa ttacttgaac ttggtgccaa agaagttatc gatcgacttc ctgttgaaga
     1441 tgatcataaa aagccactcg catcatcaac ttggcaagct tgtgtagacc ctgttggtgg
     1501 cgaaggtatt aattatgtta caaagcgttt aaatcatagt gggtcaattg cagttattgg
     1561 tatgactgcc ggtaatactt atactaattc tgtattccct cacattttaa gaggtgtaaa
     1621 cattttagga attgactcgg tatttactgc tatgaaatta agacagcgcg tttggcgtcg
     1681 tctcgcaaaa gatttaatgc ctgaaaattt acatgagatc aagcaagtta ttacatttga
     1741 tgaacttcca gaacaactta acaaagtaat taaacatgaa aataaagggc gcattgttat
     1801 cgatttcggt gtagataaat agtattcatg aaaaagacat cccgttatgc gagatgtctt
     1861 ttttaattta gtatttgata tacataccgc ctgaatctgg ttcggtaggt ataaatccaa
     1921 attttgtata taatttatcc gctgggtagt ctgcaatcag actaacgtat gtactctcaa
     1981 cagccacacc tttaatatat tgcataatat gctccataat tagactgccg taaccttgac
     2041 cttggtaact tttcaaaact gcaatatcaa caatttgaaa aacagttccg ccatcgccaa
     2101 tcactctacc cataccaatt aaccgatctt tatcatacaa ggttactgta aataaggcat
     2161 taggtaatcc tttttcagct gcttcgcgcg tctttggact catacctgcg ttaatcctta
     2221 atgcgcaata atcctcgcaa gtcggaatat catatgtcac tttaaccatt atttacccca
     2281 cttttcatca cacaatatat caacctagta taaatgttta tttacaatag tcttattcgc
     2341 ttctttaaac acttcatgat gacttgaaac ataaccctct gcattcgcat ctggttggat
     2401 atatgtttta gcaaggttcg ctgcatttgc accatcacta aatgcacttg caattagatg
     2461 tgattttgca tcatgataaa caatatctcc acacgcatag ataccaggta tactagttgt
     2521 cgtattacca aatcctttaa cacgacaatc atcatgcata tctagctttg aagatgtttc
     2581 actcaataat gtattacaac gatcaaaccc atgactaata atgacatcgt caaatttaac
     2641 tgtatgccta tcgccacttt caacatgttc caaaacaact tcacttatat gcgtttcatc
     2701 atcattgccg accaagtatt taatacgtgt ttttgggcat agtttcacat ttaaatctgt
     2761 caccaacgtt ttcatcgctt catgaccact tacatcttct tttcgataaa caactgtcac
     2821 gcttttagca atcttggcaa tatcatgcgc ccaatctaat gctgtatttc ctccacctga
     2881 tattaataca tctttatctt tgaaacgtct gtaactttgt acaacataat gtaaattagt
     2941 taattgatat ctctctacac ctttaacatc taattgtttt ggattaataa tacccgcacc
     3001 aattgcaatg ataactgctt tcgatgtata tatttctccc gcttctgttt caacttcgaa
     3061 atgacgttct gcctttttcc taatatctac cacacgttca ttcaaatgaa cttccggttt
     3121 aaaatataat ccttgcttaa ttgtatcttt taaaatttca tgacaaggtt ttggcgcaat
     3181 gccgccaata tcccaaataa ttttttcagg gtaaattctc atcttacccc ctaattcaga
     3241 ttgaacatct atcaatctta cagacatatc tcgcaatcca gcataaaagc ttgcatacaa
     3301 accagacgga ccgccaccaa tgattgtaac atctttcatt atgtgcctcc tatgactctc
     3361 tatattcatt tctttcatta acgtgctcaa attgataatt attatcattt aaagccatta
     3421 tactattaat atttatattg ttaaaataaa tcgcatagtt agccatgaat tatcaataac
     3481 tatcccatca caatataaaa acacaccttt caaagtaaga aacaacaatg ttcctttgaa
     3541 agatgtgtat attttttagt tctagttata ttatttttta aaagactcat cacgtggttc
     3601 tttaagaatt gcttgtctta aaaggaaaaa tagcaacaat aaacctgcaa gcatacctgt
     3661 gtgcccaata cctgcaaagc ctgcaaatgc ttctggagag tatgatttac cagtgacttg
     3721 gaagaatcct tttgtcacca tcatacctat agtaacgata acacctatgt tatatacata
     3781 gaagaaccag ttaaataagt agtaacttga taatttaaat aacttctcca atggtaataa
     3841 tattaaaaac ataaacatac ctaaaataag tgtatgtgtg tgtacaagtg ataattgtgt
     3901 tgtaccagta aaatcatatg cttttgttag ctctctatag aaaaagccac taaataaacc
     3961 tatgatcata taaaaaagaa atgaatataa taacctacgc attttgaatc ctccctcacc
     4021 ttttcattat atccgattat acctattata acagtgacaa aaaagcgaag atatgacaca
     4081 actgaatttg gtttagcaca tagtatgaca gctaaaataa ctttacatca agcgctatac
     4141 aaataaaata gctataccat gacgctcgct acaaacgtct tgtatagcta ttatttctat
     4201 ttattaaatt atgacggcac ttcagtacgt tttgtgctat gttttctaac aatagctgaa
     4261 actaaacttg atacagcgcc aaaaatcata aaccctataa acatccacat tgtactattt
     4321 aattcaatat gatgattcaa gtataatagt tctcttacac ttgttgcata gtgtgtgaat
     4381 ggattccaat catatacata tttttgataa ctttcaggca acatttgttt aggtaacgtt
     4441 acaagttgca tactaaagaa cattaaaatg aagaaaattg gaactgactt catacctagc
     4501 cataccataa cacctaaaat aaggcccacg aaaccaagaa tcgcaaatgc tacaaaaatt
     4561 gcgatacgat ttggatggtc aaaatcaaat ctttgaacgc cttgcatgaa ataaatataa
     4621 acaaagctac ctgcaaatgc tgcaacaact gcaaatatca tctgtccaat tgaagcaatg
     4681 atacgatgtt gcacgacgat attgttacta gttctaaatg caaagaacaa taagatagac
     4741 gttacgattg aacctatcca aattggcata aacattagga atggtgcatt accacctgct
     4801 tggtgatctt taactttatt aagtttttca ttatccactt ttactggatt cgtcaaacca
     4861 ttgatgtccg cggcattgac tttaacattt tgactcgtta atgtttccaa actttgcttc
     4921 gtaatttgag cattaatatt atcacccata ccagttaaca cagctgatgc aatttgtgaa
     4981 ccttgtaagc ttgatccttc acttacaatc gttttaaatt tagcctgctt aacctctact
     5041 tgttgattcc ccattttttg tttcatttgt tgaaccactt gtggcggaat ttcacctgaa
     5101 gcaacttttt gttgcatttc ttctttttta ctatccataa ctactttttg tgttttactc
     5161 atagcatctt ttgaaaagtc tttatcaata atagctactc caaagatttt ttgatctttc
     5221 aaatcttttt caaggtcttt ttcactatca accttaaccc atttaatttt attagaatca
     5281 ctatctaata atttatcctc taatttttta ccaatgttaa catttttatc ttgaatcgtt
     5341 gtacctttat cctcgtttaa tataccaatt ggtaaatctt ttggtttagg attatatgca
     5401 ggataaaaag ctaaagaaaa gattaccaag ataatcatag ttgctattgg tgcaatccat
     5461 aataatttat ttttaaatat attcatttta ttaattacct ccttattaga caatgtgttg
     5521 aatatttcgc atgttgtcta ttataatatg taagtgggaa taaacaatac acaatgttca
     5581 tcaatacgtt cattaataga caaatacacc cacattgttc aataacacaa ggaggttgat
     5641 ttatttttga aagaagatag gcgaattaga aagacaaagt cctccattaa acaagcgttc
     5701 actaaattac tacaagaaaa agatttagaa aagattacca ttcgcgacat aacaacacgc
     5761 gctgatatca atagagggac attttactta cattacgagg ataaatatat gttactcgca
     5821 gatatggaag atgagtatat ttcagaacta acgacatata ctcaatttga tttgttacgc
     5881 ggttcttcaa ttgaagacat tgcgaatact tttgtaaata atatactcaa aaatattttc
     5941 caacatattc atgataattt agagttttat catactattt tacaattgga acgcacgagt
     6001 caattagaat tgaaaatcaa cgaacatatt aaaaataata tgcaacgtta cattagtatc
     6061 aatcattcta tcggaggcgt tccagagatg tatttttaca gttacgtttc cggagcaaca
     6121 atttcaatta ttaaatactg ggtaatggac aaacagccca tttcagttga tgaattagct
     6181 aaacatgtgc ataatattat ttttaatggt ccattaagaa taatggcaga aaaccgtttg
     6241 cataaatcaa accttgattc actaacttaa gtaatactcg cttttcctaa atcctttaga
     6301 aaggaactat atatcatgat tacttcattc agacattctg aagatataga taaacatatt
     6361 ataaaaacac cattagatca tacagcgtca tggattaatg tagtagaacc agaccgagaa
     6421 gaaattgaaa atcttatgga acaatataat atacctgaag actttatacg tgaccctttg
     6481 gactcagaag aaagttcccg tattgaatat gacgaagata ctggttactc attaatcatt
     6541 attgatttac ctatcgtcaa ttcaactaat cgtagcgttc tatcttttgt aacgattcca
     6601 ttaggcatta ttattggcaa cggtattatc gtaacagttt gtgacgctga aaatgaattt
     6661 ttagaaaatt tacctaagcg tgatattaat ttaaaatttc acagcagatt tgcattagaa
     6721 attttaacta ctattgccga ccattataat cgtaacttac gattacttaa taaaagtaga
     6781 attcgtattg agaaagaact aaaaaataac attactaaca aacaactttt caaattaatg
     6841 gaagttgaaa aaagtttagt atacttttta gctgccttaa aaggtaacga tacaattatt
     6901 aaaaagttat tccgtttacc tgcaattaaa cgttttgaag aagatgaaga attactagaa
     6961 gatttaatca ttgaaaataa ccaagccatc gaaacaacag aattacatca acgtatctta
     7021 gaaagtatca caacatcata cgcttcctta ttatctaatg atatgaatac gattatgaag
     7081 acattaacac ttttcacggt actattaacg ttaccaacac tcgtatttag tttcttcggt
     7141 atgaatgtgc cgttgccaat tgatgaccat agttatatat cttggattat tgtggtaggt
     7201 atttcattaa ttcttgtcgt aattgttagt attttcttat ggcgtaaaca gaaattataa
     7261 ctaaattaaa aggtatgaca gcttaaagtt tcatcctatt gcatttgcaa tgactgatac
     7321 tttgattact gtcatacctc tttttttatt ctacaacttc tttatttgct tttcttttag
     7381 ataaaattaa agtaactgtt atagcaatga tgaatgatat tgtcatacca acaaagtagt
     7441 gtaaccatcc tgcatgtact ggattgattg aaataaatcc tggcaatcca gcagttccta
     7501 atgcgattgc tttaaccttg aagaaagcaa tatatgctga accaatacct gatccaacga
     7561 tagcgccaat aaatggatat cttagtttta agttaacacc aaacatagcc ggttctgtaa
     7621 taccaagtaa tgctgaaata cctgcggcag atgccacacc ttttaacttc ttattttgtt
     7681 taataataaa gaacgctgca attgctgcac caccttgtgc aacattagac attgtcgcaa
     7741 tcgggaatat aaatgatcca cccgttttag tggcatctgc aattaatgtc gtttctacag
     7801 ctataaagct atgatgcata cctgtaataa caatcggagc atacaataat ccgaatatta
     7861 atccaccaat tgcaccacca aattcataaa gccatgttaa accatctgat aaccagtaac
     7921 ctaattgtcg agtgattgga cctacaaatg aaaatgttag aaatgctgtt ataaaaatag
     7981 ataacaatgg tgttaacaaa ttatctaaca ccgttggaat aactttgcgt aacccttttt
     8041 caattgaggc taagatataa gctgctacaa gcataggtaa cacttgtcct tgataaccta
     8101 cttgattaat atgcaaacca aaaacatccc aatatggaat agccttacct gcttcaactg
     8161 cttttgggaa atcgtatgcg ctcattagcg atggatgaac tagtatcata cctaatgcag
     8221 cacctaaaaa tggattgcca ccaaagcgtt ttgctgcact aaatccaatt aaaattggta
     8281 ataatgtaaa tggtgcattc gcaaaaacat ttatcatttc agctaatcca gcaaattgac
     8341 tatatacatc tatcaatgat ttacctgaaa agaataaatc tttcgcagtc aaaatgttat
     8401 ttaaccccat taataaacca ccagcaacga tggctggtat aatcggaaca aagatatctg
     8461 aaagcatttt tacaaatcgc tgtaacggat tcatattttt agcagattgt gctttgactt
     8521 ccgaagtggt tgatgcttct tttccagtta atttttccag ttcactaaat actttattga
     8581 ctgtaccaga cccaataata atttggtatt gtcccccagt agaaaacgtc cctttaacta
     8641 catccatatt gtttagcgcc tcttcattta ctaaactttc atcatttaaa actaatcgta
     8701 gtctcgttgc acaatgcgcc attgcatcta aattctcttc tccgcctatc gcgttcaaaa
     8761 tttcttctgc ggattgctta taattcatat atttagcctc ctaattagaa ccggttccat
     8821 ttattccact ttattgtaac cggttccaaa aatatttgca acactctatt atcattttat
     8881 ataaacacct acaccatttt tgatagcgca ttcattttta ttgctatact acatgcatgt
     8941 atattaacat taattatcgg aggtaatttt atgaaagaac gtggctatat cgaacagctt
     9001 tggcgtgagg aaaaatatca tgtattgtta cacagtcaac agtcttacca aatgataagg
     9061 aatgctttaa aaacggattt atcacttcat caagttcaac aaatgattga tgttgctttg
     9121 ttaattgaac cgagtatagg aagtgtatgt aatgcttttg atcatatgtg gggatattta
     9181 aaaaaatgtg cgaatgaaga agaaagacaa caatcaaaac tacttaaagc tgattttatt
     9241 aatggtaaaa tcgacaccca aactttatta gattttttag cagaacttgc taataaatat
     9301 gatgttcaat atttactaca aagtcgtgtt ttaaacacaa aaagaaagag atagattgaa
     9361 actttagttg taacggacaa atagtagtca ttggagttca gcaattcata ctttgtacta
     9421 aagtttacta taatctatct ctcaaggttt aaatgtacat atctatatta aaatgcgttt
     9481 catgatgtta gaagatacca tacacattta attagttaca gcataaatca atttataccc
     9541 ttaattatta cattgtttat attttaaatt cgggggagta attttaagta atatcttgtt
     9601 gctgctaatt ctattgttaa taaattgata ttagatgtag aaattgagtg tgaaagttaa
     9661 taatagatga tttagctagt aacataatta attcatatat agtcaaaaag ttctgttaga
     9721 tactgttctt gatgaatatt ctttgatgtt aggtgttagc tattctagat gagtttctta
     9781 gatgtggatg cctgtcttga tgaagttatc tatgatgtta atataatact tgatgttgat
     9841 tcttagatgt tagtgcgatt tcaagatgct gattctttgt tgttgcacta tcttggtgac
     9901 gtttctagtt gtttatagtt atcgcatctc ttgatgtaaa atctaagatg tttatgcatt
     9961 tctagatgtt aattcttcgc tgcttaagaa ttatcttgat ggtaaatcta gttgtgccat
    10021 gcattttctc atgatgaatc taaggtgtta attcattgat gtgtgctgtt tcttgttgct
    10081 tcattatctt gatggtgaat ttcgttgtct aatgcacttt ttcaaatgat tattctaatt
    10141 gttacagcaa taactagatg gattttctta gatgttcact tttctagatg tttatactta
    10201 tcttgatgtt ttctaaacag aacttttaaa cgctttattt tttagtagat tgtttaaaat
    10261 ttcttagttg atggttattg ttgttgattt gaataatttg tagcgaatat tttgtaacag
    10321 ggctactaag aatttttata acaagaagtg atttcaatcg tactaatttt cagcgtttga
    10381 tgacaaaagt gtatgtccaa acttaaataa tggcgataaa atttaagtca tgggtacatt
    10441 taatgttttt tgattcaaac ttccattttg gtcaatttgc tttttttata acatagttga
    10501 atatcaataa aagattagca agttagttca aaatggctat gtattgtttt ttgaaataca
    10561 taggtttgaa atctatatta attatgcacc aaataattta attagactct atcgaaaatt
    10621 tccaaatttt gcttactatc tttctaattt tacttttata ttgttcgcaa tgcataaaaa
    10681 caagcgtttt cctaggagtt ttcattaatt tcttttattt ttcactatta ccttttcaaa
    10741 aatattcaac tataataggt atactatcca tgattttaaa aatttccatg ttttacattt
    10801 aaatgataga tttcaatata tattaggcag gagagagaaa tatgacatgc caacttaaaa
    10861 tacatcatac attatcgaca ataccaagtc gtaatattaa taatattatg gtgctatttt
    10921 cacttacaag taaactcaat attacgatta atggtgaaac caaagacgta agtaattata
    10981 ttatcttaat taaccatggt gatatttata atattaacca cggcgaaaat ataatcgaat
    11041 taatgatacc cgttttttat ttttatcaac aagatgatga tttctttaat ggttatttag
    11101 atcgtcattt attacaatca agtaattata ttaagtcatt aattgccgat ttaatcagta
    11161 caccaacaag ttcttcttta atggggaaaa atataggtca aagtattata gacactttac
    11221 ttaaagaagc ttttataaga atagatcatg aatatttacc aaatattgct ttaagcaatc
    11281 cggtgttcat agactgtgta aactatattc atgacaatat tgatgcgcac ttgtctttaa
    11341 aagatatagc gatgcattgc aacatctctg aatcatattg ttctaatctt tttgttcgat
    11401 atttgagtat gaattttaaa gattatttta caagtattaa acttgttaac gccatcaatt
    11461 tattactttc tacaaaacat tctattacaa ctgtttctga attggccggt tttaacagtc
    11521 atacaaattt tgcaaatcaa tttaagaatt acttgcattt tagtcctaaa cagttccgtt
    11581 cactcgtttc caagattact gaaccaccac aaatacactt ccaacaagat aacgtatcac
    11641 aattcactga gttaatttca acaattgatt taacagctca actggctaca aatacaacag
    11701 atattcatat agatgatttt aatccgaaag atcgaagtca gcgtgccaaa gtatttgttc
    11761 gatttagtaa tttcaatgaa ctatttcaat ttattttcaa tgaatattac gatattaact
    11821 ttgagcactt accaaaacct gtcgttttca ttgacgatat tcacgatatt gagattagcc
    11881 aaacgaatta caacctgtta aatagatgtt ttgaaaagct tttcgaaaaa aatataggtt
    11941 tagcaatcgc tattaaatca acacaacaat tcgaaacgat gaagcaatta atactaacat
    12001 ttttacaagg caatcaagac tataagacaa gcaagaaatt agttaaattt atgctcgtat
    12061 tttgctctaa ttctatgaca gcagaagaaa ttcatttatg tcatcttaaa attaaaaaca
    12121 aaaataaaga aattaaatat agtgtgacag tcgatggctt tttagaaact tattcaaccg
    12181 tggaacaagt ttatgatgtc atgcagcgtc ttaaattcca ttattacttt attgatattg
    12241 aaaattcaaa aactgcaaca catcttatca ctaaaaacca acactatcat caaactgaca
    12301 ctcattttga acagtataag aaatttattt tagattcagg tatatcatca acacaatttg
    12361 tatataataa cctgtctgta agcggattta aatatactaa tgatggtaag aatccaattc
    12421 aattatctga catagtgtat cacttaatcg cattattacg atatggcggt ggcattagct
    12481 atcaattatt agatgaccat tcaaattata tttccttgta caacaaatat ggtagccccc
    12541 taccattaat gcatttatat aaaatgttta gaccttttgt taatgaagat attgaaatta
    12601 caaataatta tgtattgagt cgtaaagata ataactacca tttcttat
//
LOCUS       DAKTGS010000081        12600 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.081, whole genome shotgun sequence.
ACCESSION   DAKTGS010000081 DAKTGS010000000
VERSION     DAKTGS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.081"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            127..942
                     /locus_tag="O0286_001804"
     CDS             127..942
                     /locus_tag="O0286_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HCY0170181.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETELTYN"
     gene            1264..2442
                     /locus_tag="O0286_001805"
     CDS             1264..2442
                     /locus_tag="O0286_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCY0170182.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
     gene            2702..3394
                     /locus_tag="O0286_001806"
     CDS             2702..3394
                     /locus_tag="O0286_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0170183.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            3391..4944
                     /locus_tag="O0286_001807"
     CDS             3391..4944
                     /locus_tag="O0286_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0170184.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            4947..5903
                     /locus_tag="O0286_001808"
     CDS             4947..5903
                     /locus_tag="O0286_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170185.1"
                     /translation="MLKRTIKFILYLIVSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            5903..6670
                     /locus_tag="O0286_001809"
     CDS             5903..6670
                     /locus_tag="O0286_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170186.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            6637..7404
                     /locus_tag="O0286_001810"
     CDS             6637..7404
                     /locus_tag="O0286_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170187.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            7397..8032
                     /locus_tag="O0286_001811"
     CDS             7397..8032
                     /locus_tag="O0286_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0170188.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
     gene            9052..11115
                     /locus_tag="O0286_001812"
     CDS             9052..11115
                     /locus_tag="O0286_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0170189.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            11133..11678
                     /locus_tag="O0286_001813"
     CDS             11133..11678
                     /locus_tag="O0286_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HCY0170190.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            complement(12106..12240)
                     /locus_tag="O0286_001814"
     CDS             complement(12106..12240)
                     /locus_tag="O0286_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170191.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            12204..12296
                     /locus_tag="O0286_001815"
     CDS             12204..12296
                     /locus_tag="O0286_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170192.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
BASE COUNT         4549 a         1818 c         2308 g         3925 t
ORIGIN      
        1 taaaaaacat gggcttaaag ggttaattta tatagtatta ctgcaaacat tgatttgggt
       61 caaaaattag gggtattaaa aaaatgaatg aatcataatt ttatcaagct gattggagag
      121 gttaaaatgc attatataaa atttattgag tcaaaagata atacaaaact ttatatgaaa
      181 gtgaatgata ttcaagatgc aaaagcgaat atcattatag ctcatggtgt ggcagaacat
      241 ttagatcgtt atgatgagat aacagcatat ttaaatgaag cgggttttag tgttattaga
      301 tatgatcaaa gagggcatgg tcgttctgaa ggcaagcgtg ccttttatag caatagtaat
      361 gaaattgtcg aagatttaga tgcgataata aattatgtga agtcaaactt tgaaggtaaa
      421 gtttacttaa tcggtcatag tatgggtggt tatacagtca ctttatatgg aacgaaacat
      481 ccaaatacag tgaatggtat tataacttct ggagcattaa cacgttataa taataaacta
      541 tttggcaatc ctgatagaaa catatcacct gatacttata tagaaaacaa tttaagtgag
      601 ggggtatgtt ctgatttaga ggtaatggaa aaatataaac ttgatgattt gaatgcgaaa
      661 caaatctcta tggggctcgt cttttcaata atggatggtg ttaggtattt gaaagacaat
      721 gctcaacaat ttacagataa tattttgata ttgcatggca aggaagatgg gctagtaagc
      781 tatgtagatt ctttacagct ttatcaagaa ataggatcag cacataaatc attacacatc
      841 tatgatcgtt tggagcatga aatatttaat gaaagttctt ataatagaac tatttttaac
      901 gaagttattg aatggcttga aacggaatta acttataact aaaacagtat agttccgtgt
      961 atttgattat aagaaattat gaggatatta aacatactaa gattagctat gaagaaatct
     1021 atgacgatag attttttcat agctattttt tatagttata gagaggagta gactgtccag
     1081 actcttggat tttaaatccg taaaaaaaac aagtcagctt tactctcacc ttttgaaatt
     1141 cgtttgtagt atgttgggtt cttgaaaccg tgtataggaa aatgaaatga gaaaggttaa
     1201 gtaaagtttt tagcttctca actattcaaa ggaggttttt ttatcgatta cttaggtgtt
     1261 gatattagta aaaggagtag tgtagttgca cattataaaa atggaaaatt ccaaaaagag
     1321 tttttcatcc aaaataataa aaatggctac aattatttac tcaagtattt gaatgactta
     1381 gaccacccac aactcatttt tgaatctaca ggtatctatt caagaggtat ggaacgattt
     1441 tgttgtgtaa atcaaattaa ctatattcaa atgaatccgt tagaagccaa atttaaaacg
     1501 agcgctctaa gatcatggaa aactgatcag gcagatgctc ataagcttgc ttgtttagga
     1561 ccgacgctca aacaaacagg cagcttacct atacatgagt taatattctt tgaattaaga
     1621 gaacgtgccc gttttcatct agaaatcgag aatgaacaaa atcgacttaa atttcagatt
     1681 cttgaattac tccatcaaac attccctggt ttagaaagat tatttagtag tcgatattca
     1741 atcattgcac tcaacatcgc agaaattttt actcatccag acgtggttct tgatatcgac
     1801 aaggatgtac ttattacaca tatattcaat tctacagata agggaatgtc aatggataaa
     1861 gctacaaaat atgcacttca attaagagtg attgctcaag aaagctatcc taatgtcgat
     1921 agacattcct ttctagtcga aaaattacgc ttacttattc aacaattaaa acaatctatt
     1981 catcatctca aacaattaga tgatgccatg attcaattag cacaacaact cgattatttt
     2041 gaaaatattc attcgatacc tggtattggt aagctaagca cagctatgat tattggggag
     2101 attggtgata ttaagcgatt taaatcaaat aaacaactca acgcttttgt aggcattgat
     2161 atcaaacgat atcaatcagg tcatacacac tgtagagata ccatcaacaa gcgtggtaat
     2221 aaaaaagcga gaaaactttt attttgggtg attatgaata taataagagg gcagcatcat
     2281 tatgacaatc atgtcgtcga ttattactac aaactaagaa agcagcctaa tgagaaacct
     2341 cataagactg ccatcattgc ttgtataaat cgattattaa aaacgattca ttatctggta
     2401 atgaatcata aattgtacga ttatcaaatg tcaccacatt agccaaacgt acaattaaat
     2461 atattttaat accttattca aaaaattaaa atgaacggtt tagttaagta atgcttattt
     2521 taattataag tacttgacta atcgtaagaa agagcctagg acataaatca atgtctcgcg
     2581 accacagctt aatttttggt gttcattcat gactttaaaa aatccttatt gcataaatgt
     2641 acatagtgta gtactattca aaacgtaatt attacgattt gaattaagcg aggagaatga
     2701 aatgactaag acttatgacg tttggtggca aaaaggtcaa gaatcagatg atgatatggc
     2761 acgagaccat caagaagctt gggagagaac aataaaaatg cttgatacat ctgacatcga
     2821 agggaaaacg attttagatg tgggatgtaa tcaaggcgga tttttacgac agttatacga
     2881 tacaacaccg tttaaaaaag gtgttggcat agatttagca cgtttatctt tggaaaaggc
     2941 agagacatta aaaggacaac gtccacttac atactattta acagataaac cgcaagaaac
     3001 gaagcacgtg tttgatacgg cagtaagtac gtctgtcttg tacttaatag aagatattcc
     3061 gcaacatgca aaagatttaa aagaggtatt gaaaccaggc ggtgtttatt acgcttcatt
     3121 cgcggattta actaataacc caagtcgtca gtttatggat gacacgatta atcaatatgg
     3181 tgcaacacct tctcagaatc actctctaaa acatatcgtt gatagctttg tggatgcagg
     3241 atttgaagtt gcagtaatga aagagcatgt acctgacgtg attgatttaa cacattatag
     3301 cgatttttat ttatcaccga atgattattt acaaacacta tatgaagaat cgtttttaat
     3361 aaaagcaagt gtgaaagaag gtactgagaa atgaggaaat gtgtattaat gacggtagca
     3421 gcaagtgcta cgctcttatt ggcaggttgt ggcaatggtc aaaaagaaga taaagatgtt
     3481 acggtatcgc tacctactga agcaaaggcg gataaacttg acgcgcaagg ctatgatgca
     3541 gcgatgcccg tttatagtgc agtgtatgat gcattagtta aatatgataa agataagggt
     3601 attaaagcag gtttagcaga taaatggagc gttgatgaat cagggaaagt ttatgaattc
     3661 catttgaaaa agaatgttaa attctcagat ggttcagcat tagatgctaa ggacgtgaaa
     3721 ttctcgattg atcgtgcgaa agcgatgaac aaagattcga ctgtagaaac gttaaaaaaa
     3781 ttagataagg tcgttgttaa aaatgagcac gtggtccaaa ttagattgaa atctccttca
     3841 aatcaagtgt taaatgaatt aacacaagtg agaccgttgc gtattatgag tccacattca
     3901 gtagaagatg gtaaagtaaa cggtaaattt gaaaaagcga ttggaacagg tgcatttgtt
     3961 gttgataaaa ctggtaaaga aaaaacgaca atgaagccaa ataaatattt tgacaacggt
     4021 cacccagtca attatcatct tgcattccaa acgattgaag atggggactc aagaaattct
     4081 gcagtacaaa gtggttctgt agatatttct ggtggtgctt taggtatgct ctcagacgaa
     4141 caaatcaaac aagataagaa aaataagaac ttaacgattg aagatagacc tagcacagta
     4201 agtcacttta tggcatttaa ccctaaaaat gatgtattaa atcaacgcac aattcgtgaa
     4261 gcgataagta agagcatcga tgcgaaagac attgcgggca aatctgtaaa tggtctgttc
     4321 cagaagaacg tacaatttgt gactaaaaat aatcaacagc cacacgatta tgatatgaaa
     4381 gcggctgaaa ggttacttaa atcagaagga tatcataaaa acgatgacgg catctttgaa
     4441 aagaatggca aacctttatc atttaactta gtcattcaaa ctgcagagtt cccaaattgg
     4501 aaagataaag ctgaaaaagt gcaacgtcag cttaaacaag ccggtattaa gttaaatgtg
     4561 aaaacgttag attcacaatc atactatgat acattatgga cgaaaaaaga ctatgatttg
     4621 attttctata gaacgtattc agatgcatta atgccttaca actttataag ttcagtgttt
     4681 aaaaataatg atggtcaacc aggggtgtta gctgatgatg aaacattaac gaaacagcta
     4741 gacgatttcc catcaaccgt atcaaaagaa gaccaacagt gttcatttga tgacatattt
     4801 aaacacttta atcaacaata ctatggtgtg ccaattgctt atccaaatga gacgtttgta
     4861 gtgagtgata aagtaaaaca attcaaattc tctggactta cggatgcacc aattgattat
     4921 aaagcgttga aagttaatga atagcaatgc tcaaacgtac aattaaattc atactttatt
     4981 taatcgtaag ttcgtttatt atcttcattt tagttgagaa gacatctggt aatccagcga
     5041 ttctgtatct acaacgtcat ggttatacgt cgattacgca agacaatatt gaagcggcac
     5101 aacatcaact tggcttagga caacatgtgt tactaagata tatcgattgg gttggacatg
     5161 cactcacggg caacttagga tacggcttta gtacgaacga agcagttacc gctatgataa
     5221 tggaagccat cgtgccgacg cttgtgctaa tcattgtctc tagttgtatc atgttgccat
     5281 ttggctatat tgttggttac ttcgttggga cgcgtccgca tacacgttac gctaatggaa
     5341 ttcgtggatt cgcccaagtg atgacctcaa tgccagaata ctggttagct attttattca
     5401 tttattattt aggcgtacgt tggcaattgt taccatttgt aggtagtgat tcatggcaac
     5461 actttgtgct gccaatcttc acaattgttg ttatagaagg gtgtcatatc ttattgatga
     5521 cagcacatct gattacacaa acgttagatc aagatgcgta tcaactggcg cagttaagac
     5581 atttttcgtt aaaagcgcgt atcatcgtac aaattaaaga gatatttgca ccactaatga
     5641 cgatttcaat taacagtatc attcatttaa ttggaaaagc cgtaatacta gaagtcatct
     5701 tcagcatgtc tggtataggt aaattgttga ttaatgctat taaccaacga gattatccac
     5761 tgattcaggg cattgtcatc tttatcattg tctttattat gctaatgaat tatttaggcg
     5821 atgtgattat tttgaagaat gaacctagac ttcgacgacg tcatacccag cagtcaggca
     5881 atgagaaaag aggtacgatg tgatgaaaaa atatcaaacg tacatcgcaa taggttcact
     5941 attgagtttg atggttgtat taattacgta tggtttaatg caagacacgc aacatttgaa
     6001 cccacttgag tcacctaatg gacaacattg gttgggtacc gatcaattag gcagagactt
     6061 cttagtaaga ctgattgtcg gtagtcttgt cacattgagt ttaacaggca tagtgattct
     6121 attaagcgtt tgtatgggac ttatctttgg cttaattgca ggcatagaaa gacgatggtt
     6181 agatcaaatc atcatgtttg ttgccgatat gttgctggct attccgtcat ttattatcgc
     6241 attagtcatc ttaagtttag taagtaactc catgataggt ttgatacttg ctttaacgat
     6301 tggatggata ggacgttatt tacgttactt cagaaattta acgcgagata ttcaaaaacg
     6361 tccatttgtt caatatgcac gattgagtgg gaactcaaca ttcaaaacga cagtaacaca
     6421 tgtgattcca catttattaa gtagtatatt cgctttggta acggctgact ttggcaaaat
     6481 gatgctcagc atatctggac ttgcttttct aggactaggt attaaaccgc cgacgcctga
     6541 gttaggaaca attctttttg atgggaaaag ttatttcaac ggcgcaccgt ggctcttctt
     6601 cttccctggt gtattgttag gaggtttcgc cttattatgt caaattatca acaaaaaaat
     6661 aacgcagtaa atacggtagt caacgtcaat caattatcga ttttagatca agagaaagta
     6721 ttgttaaagg atgttgattt gacagtaact aaaggtgcat ttcattgcat tataggtgaa
     6781 agtggcagtg ggaaatcact gttaacaaga acaatacttg gaatgaaaca atcacaatta
     6841 tgttatcaag gagatattga catcgattta actcaaacag atgcagtgtt tcaagatgtt
     6901 caaagtaata tgtttcaaaa tataacatta gctaagcatt tccaatacat ttatgaagcc
     6961 aatcgcacac atctcactaa acagcgtatt aaggaagatg tcttacagat gatgcaatta
     7021 cttggtttaa gacaagggga acaattgctt gagcgttatc ccttcgaact tagtggaggt
     7081 atggcacaac gtgtcgcctt tataatgtca ttaattagac gtccgaacta cttattttta
     7141 gatgaaccaa cgagtgcact tgatcaagaa aatattaaaa agtttatgca ttaccttctt
     7201 agggcacagg agcgctacca aatgaccatt gtttttatca cacatgatat taacttagtg
     7261 aaagattgtg ccacacatat tagtattatg cagcaaggta aattgataga aaatggtgag
     7321 gccgcgtcga tcttaactaa gccgacacat aattacacga aaaaattaat tacgattgca
     7381 catcggagac aaccttatgc ttaaaataga gagattaacc aaatatatag acacgcaact
     7441 gatatttaaa gagatatcat gtacaattaa cgaccagcac ttactcataa gtggggagag
     7501 tggttgtggt aaatccacat tagccaagat tatcgctggc ttagatacgg attatcaggg
     7561 cgaattatat cttaatgggc gcttacgtga atcttatacg tctaaagagt ggatgaagca
     7621 catccaatat gtacctcaat atcaacgtga tactttaaat cagcgtaaaa cggtattagc
     7681 tacattatta gaaccactta agaattataa ggtaaataaa cagcgttata catcaagcat
     7741 tgaagcagtg cttgatcagt gtaatttacc acacgatata cttaatcata aagtttcgac
     7801 attaagtggt ggccaatttc aacgcgtctg gatagctaaa gctttaatat tagaaccaga
     7861 gattctcata ttggatgaag ctacaaccaa cttagatgtc attaatgaag aagctatact
     7921 tcaaatgttg atttccttaa agatgacaca attaatcatt atttcacatg atacatacgt
     7981 cttaagccaa tttgaaggaa ttcagttaca gctaaataaa ttgaataatt aagatcacaa
     8041 atcttaatat ggtgaatatt taatggtacc taaaaaataa aatttaaact acaatgtcta
     8101 aatccatatg ttgtttcatt agaggattta aaaatgatta taacactaaa agatttcaaa
     8161 ttattatatt taatataaat ttacatatga taaacgaata acaattccaa tataaattat
     8221 tttttgatta tttttattta taactatata ttttatatga aaaatataag ttataataat
     8281 aagtttaata ttgcctcgtg gttctgagct tgaacttatc tctaaatcat tttgagctac
     8341 ttatctatca attcatatat tctataacaa tatttgtgac atcacgtgct atttcatgaa
     8401 gtgattttac gatatcacct tctttagaaa aaatatattt cagtaggcac cgacgtatac
     8461 agaatcattt gagtattaaa aataaaaact agaaaaagaa accgcatcat taactgatac
     8521 gcagaatcat attataaata aaactaaaaa tgaggttgta tataactcac tctgaaattg
     8581 attgaatata tagtatcttt aataaaatgc agctattgtg gcgtagaatt tgagaatcaa
     8641 aaaatgatta atatagtttg aagagactga gcataaatac tagaaaaatg gccagtaaat
     8701 gagtttacta taaactcatt tactggcttc tctaataatt atcaagacaa tttgcgtttc
     8761 taggcatact ttgaaatgcg ctattttctt taagaatatt aatatatgac ttgtgttggt
     8821 aatgataacc ttggttagag tgtattgtca tgtgatagtt aagattagat cttttaatta
     8881 aaacattgtt ttaaatatcg atgacaaggt ctaatgtagg acgtgtagat ataaggttcg
     8941 gaattatata aatccataaa aggagataaa tcgaaaacct ttataccctg ggtgggtata
     9001 tgttatagta taagtaactt tactataaca ttttcattag gaggggttaa tttgaataat
     9061 aatggtgaag agcataatca tcaaaatcac atgaatcatt ccaatcaaat gcatcatgat
     9121 aaccatgcct cacatgatca tcatagtggc catgcacatc atcatggaaa ttttaaagtt
     9181 aagttttttg tttcattaat ttttgcaata cctatcattc ttttatcgcc actgatgggt
     9241 gttaacttac cttttcaatt cacatttcca ggttctgaat gggtagtgtt aatattaagt
     9301 acaattttat tcttttatgg tggtaaaccg ttcttgtctg gtggtaaaga tgaaattgct
     9361 acaaaaaaac caggcatgat gaccttagtt gccctaggta tttcagtagc ttatatttat
     9421 agcttgtatg ctttttatat gaataacttt agtagtgcaa ctggtcatac aatggacttt
     9481 ttttgggaat tagcaacctt aattttaatt atgctattag gacattggat agaaatgaat
     9541 gctgtcggaa atgctggaga tgctttaaag aaaatggcag aactgttacc taatagtgct
     9601 attaaagtta tggataatgg ccaacgcgaa gaagttaaaa tatcagacat catgactgat
     9661 gatatcgtcg aagtaaaagc cggagaaagc attccaacag atggtattat cgttcaagga
     9721 caaacatcta tagatgaatc cctagtcact ggagaatcta aaaaagtaca aaaaaatcaa
     9781 aatgacaacg tcatcggggg ttctattaat gggtctggaa caatacaagt caaggttaca
     9841 gctgtgggag aagatggata tctttctcaa gttatgggac ttgttaatca agcacaaaat
     9901 gataaatcta gtgctgaatt gttatctgat aaagtagcgg gttatttatt ctactttgct
     9961 gtaagtgttg gcgtgatttc ttttattgtc tggatgctca ttcaaaatga tgttgatttt
    10021 gcattagaac gtcttgtaac tgtgttagtc attgcttgtc cacatgcttt aggcttggca
    10081 atacctttag tcactgcacg ttctacttca attggtgcac ataatggttt aattattaaa
    10141 aatagagagt ctgtagaaat agctcaacat atcgattatg taatgatgga caaaactggt
    10201 actttaactg agggtaactt ttctgtgaat cattatgaga gctttaaaaa tgatttgagt
    10261 aatgatacaa tattaagcct tttcgcctca ttagaaagtc aatctaatca cccattagct
    10321 ataagtattg ttgattttgc gaaaagtaaa aatgtttcat ttactaaccc acaagacgtt
    10381 aataatattc caggtgtcgg attagaaggt ctaattgata ataaaacata taaaataaca
    10441 aatgtctctt atcttgataa acataaactt aattatgacg atgacttgtt tactaaatta
    10501 gctcaacaag gtaattcaat cagttattta attgaggatc aacaagtcat tggcatgatt
    10561 gctcaaggag atcaaattaa agaaagctca aaacaaatgg tagctgattt actatcaaga
    10621 aatattacac cagtcatgct tacaggtgac aataatgaag tggcacacgc tgtcgcaaaa
    10681 gaattaggta ttagtgatgt ccacgcacaa ctcatgccag aagataagga aagcattata
    10741 aaagattatc aaagtgacgg taataaagtc atgatggtcg gagacggtat caacgatgcg
    10801 ccgagtctta taagagcgga tattggtata gcaattggtg caggtacaga tgttgcagtg
    10861 gattcaggtg atatcatact tgttaaaagt aatccatcag atatcattca tttcttgacc
    10921 ctttcaaata atactatgag aaaaatggtg caaaacttat ggtggggtgc aggttataat
    10981 attgttgctg tacctttagc agctggtatt ttagcattta ttggcttgat tttatcacct
    11041 gcaataggtg ctattttaat gtctttaagt acaattatcg ttgcaattaa tgcctttaca
    11101 ttaaaattaa aataaaagat aggagtttta ttatgattaa aaaattattt tttatgatat
    11161 taggatcatt actaatatta tcagcttgct ccaataatga tgaaaaagat aaagacacta
    11221 atgaccaaaa aagtgagagc catatgaagc ataatgatga aagtaaagtt ccagaagata
    11281 tgaaatcgac taatgagggt gaatttaaag tgggagataa agtaacgatt acagcagggc
    11341 atatgccagg tatgaaaggt gcagaagcta ctgtaaaagg tgcgtataaa acatatgcct
    11401 atgttgtaag ttataaaccc acaaatggaa atgaaaaagt aaacaatcat aaatgggtcg
    11461 taaacgaaga gattaaagat gcacctaaag atggatttag taagggcgat actgttaaat
    11521 tagaagcaag tcatatgtct ggtatgaaag gtgctacagc caatatagat aacgtgaaaa
    11581 agactactgt ttacgtagtt gattacaaat ccaaagataa tggtaaaatc attaaaaatc
    11641 ataaatggat gacaggaaat gagctgaaag cacgataaaa atctagttct aaattgagaa
    11701 ataaatagat ataaaaatat cctccttaat caataattta aataacttat tattgttaag
    11761 gaggatattt tttagtgtgt aaattaaaaa gaattttaga agaataacat ttatcaaaaa
    11821 actgttcatt accttattaa ttgaaattat ataattaaaa accgcatcat taaccgatac
    11881 gcagaggcgt atcataagta aaactaaaaa attctgtatg aggagataat aatttggagg
    11941 gtgttaaatg gtggacatta aatccacgtt cattcaatat ataagatata tcacgataat
    12001 tgcgcatata acttaagtag tagctaacag ttgaaattag gccctatcaa attggtttat
    12061 atctaaaatg attaatatag aatgcttctt tttgtcctta ttaaattata aaagtaactt
    12121 tgcaatagaa acagttattt cataatcaac agtcattgac gtagctaagt aatgataaat
    12181 aatcataaat aaaattacag atattgacaa aaaatagtaa atataccaat gaagtttcaa
    12241 aagaacaatt ccaagaaatt gagaatgtaa ataataaggt caaagaattt tattaagatt
    12301 tgaaagagta tcaatcaaga aagatgtagt tttttaataa actatttgga aaataattat
    12361 cataatttaa aaactgacaa tttgcgagac tcataaaatg taataatgga aatagatgta
    12421 aaatataatt aaggggtgta atatgaagat taatatttat aaatctattt ataattttca
    12481 ggaaacaaat acaaattttt tagagaatct agaatcttta aatgatgaca attatgaact
    12541 gcttaatgat aaagaacttg ttagtgattc aaatgaatta aaattaatta gtaaagttta
//
LOCUS       DAKTGS010000082        12344 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.082, whole genome shotgun sequence.
ACCESSION   DAKTGS010000082 DAKTGS010000000
VERSION     DAKTGS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.082"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..901
                     /locus_tag="O0286_001816"
     CDS             <1..901
                     /locus_tag="O0286_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0170193.1"
                     /translation="GNKPDLIKRIDDNFHIINNLDLPYVYIPTKKGEEILKKTEYLTS
                     FIQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEFQRKYDNGEFDFNHGYNFELNM
                     LIDHYKRDVKDYDNARKYSNIYLYFGLRDFLKKLMSNYSYYDSKGNIDLNEIQNDLNR
                     FINSSASGMYERLIYNENLSNNIMFELFKKDTQDYSDLEEQLIEKFINYVVSNVKKES
                     RSNTLIELSKILENGYTIDKEEFKKEDDYLSKYIFTDIDYLKKLESKINVAIDIRSGE
                     IHLVLDDDSLDILIQNQKYGNEF"
     gene            944..1427
                     /locus_tag="O0286_001817"
                     /pseudo
     CDS             944..1427
                     /locus_tag="O0286_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1588..1692
                     /locus_tag="O0286_001818"
     CDS             1588..1692
                     /locus_tag="O0286_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170194.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(2192..3283)
                     /locus_tag="O0286_001819"
     CDS             complement(2192..3283)
                     /locus_tag="O0286_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0170195.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(3549..4529)
                     /gene="czrB"
                     /locus_tag="O0286_001820"
     CDS             complement(3549..4529)
                     /gene="czrB"
                     /locus_tag="O0286_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCY0170196.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(4531..4851)
                     /gene="czrA"
                     /locus_tag="O0286_001821"
     CDS             complement(4531..4851)
                     /gene="czrA"
                     /locus_tag="O0286_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCY0170197.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            5003..5668
                     /locus_tag="O0286_001822"
     CDS             5003..5668
                     /locus_tag="O0286_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0170198.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            6103..7041
                     /locus_tag="O0286_001823"
     CDS             6103..7041
                     /locus_tag="O0286_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCY0170199.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            7072..7302
                     /locus_tag="O0286_001824"
     CDS             7072..7302
                     /locus_tag="O0286_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170200.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            7974..9347
                     /locus_tag="O0286_001825"
     CDS             7974..9347
                     /locus_tag="O0286_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCY0170201.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            9805..10218
                     /locus_tag="O0286_001826"
     CDS             9805..10218
                     /locus_tag="O0286_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCY0170202.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            10418..10861
                     /locus_tag="O0286_001827"
     CDS             10418..10861
                     /locus_tag="O0286_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCY0170203.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(10982..11692)
                     /gene="deoD"
                     /locus_tag="O0286_001828"
     CDS             complement(10982..11692)
                     /gene="deoD"
                     /locus_tag="O0286_001828"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0170204.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            12007..>12344
                     /gene="deoC"
                     /locus_tag="O0286_001829"
     CDS             12007..>12344
                     /gene="deoC"
                     /locus_tag="O0286_001829"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0170205.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
BASE COUNT         4505 a         1670 c         2183 g         3986 t
ORIGIN      
        1 aggtaataaa cctgacttaa ttaaacgtat tgacgacaat tttcatatta ttaataactt
       61 agatttacca tatgtatata taccaactaa gaaaggagaa gaaattttaa agaaaacaga
      121 gtacttaacc tcttttatac aaagttatgg tgaaatttct cttgagcgtg cttattattt
      181 ggttgaaaac tatatagatg aaaattgcga tgataaagtt gcagaaatat acaagtttga
      241 atttcaaaga aaatatgaca atggcgagtt tgattttaat catggatata atttcgaatt
      301 gaatatgttg atagatcact ataaaagaga tgtaaaagac tacgataatg ccagaaagta
      361 ttcaaatatt tatctttact ttggtttgag agatttctta aaaaaattaa tgagtaatta
      421 ttcatattac gatagtaaag ggaatataga tttgaacgaa atacaaaacg atctgaatag
      481 atttatcaac tctagcgctt ctggtatgta cgagcgatta atatataatg aaaatttatc
      541 caataatatt atgtttgaat tatttaaaaa ggacacacaa gattatagtg atttggaaga
      601 acaattgatt gaaaagttca taaactatgt agtgtctaat gtaaaaaaag aaagtaggag
      661 taatactctt atagagttat caaaaatttt agagaacgga tatacaattg ataaagaaga
      721 atttaaaaaa gaagatgatt atctttctaa gtacatattt actgacatag attatttgaa
      781 aaagttagaa tcaaaaataa acgttgctat tgatattcga agtggagaaa ttcatttggt
      841 attagatgat gatagccttg atatattaat acaaaatcaa aaatacggca atgagttttg
      901 agtcataact aaatataata tgttgaagag aggttttatt taaatggcta aaattggtta
      961 tgatcgtgta tcaacgaaag attaatatcg atacacttaa agaatttggg tgtgggtgtg
     1021 tatatagtga aagaataagt ggtgtcaaac gtacagagct tgttaagtgt ttagattatt
     1081 tacgagagag cgacacatta gttgtctatc aacttgatcg gttaggtaga acgacaaaac
     1141 tattaattga attatcacaa tgattcgatg ataacggaat tgacttacaa attagtaaca
     1201 tgaacatttc aacgaaagac acaatgggca aaatgttttt tacgatgatg agtgcatttt
     1261 tcaggattag aagttaattt actatgtgag tgtataaaat agacttagca gcaacaagag
     1321 cgagaggccg aaaaagcggg cgcccctctt taccagagaa taaaaaatga gaaattaaat
     1381 ttttatatga tgaacaaacg ataacagggg aagaaatagc tagttagaca agggtatgtc
     1441 actcaactgt ttatcgagtt gattaagaaa tgaaaaaact tatactatga attactgttt
     1501 aaaagtgtgc atgttataat atttattgag caagttggat agatggtggc taatctctta
     1561 ataaaggggt gatgcctatg gttatagttg ttactcctag aaaggactag catgtctgat
     1621 tttgaaatgc ttatggttgt attaacaatc attggtttag tattgattag tactcaagac
     1681 cataaaaaat aaccttctat tcgctttgac cggcattttt gaaggctatt tttaaataaa
     1741 atataaggtc accgtctttt taacgggctc attagggtaa catgtttccg agtgttgccc
     1801 tttttgtgtt tcaagagtta atgattttca tcttttgctt ctacttacta caaatatatt
     1861 ttaacacatt tttcttatga attgtagttc tgaacataat cagaattaat aaaaccaact
     1921 ttccatacag cagaaaatac aattaaaagt atagaatgta atcacgaatt atataaaaag
     1981 tattggagac ctcttcatat atagataatt cacttagtta ttttagaaag aagcccctaa
     2041 caactaaagt tgaaaaatag aggaacacag ttggatgcta cgcatcaact gcataagagc
     2101 ccctaacaac taaagtttaa aagtaggagc gcctgcgcta agcgcatgca taagagcccc
     2161 taacaactaa agttgtaagg ggctctaaaa tttattttgg ttgattgtct tctggtttat
     2221 ctgaagtcat tgtttttgtt gtattatcat ttaatgaatc ttgtgctttt tctgtttttg
     2281 tttcaacaga tgttgtcact ttatcatttt ctacttttgt attttttggt tcagtgacaa
     2341 cttttttatt cttgtcagct gtttttactt tatctttagt aattttttga ccacgtttta
     2401 agtaatattg aacgataccc attaaaatga ttgcatgaat aacaacgaat aaaataattg
     2461 tcattactgt ataaactctt atgatatttt ctgcaacact ttgagagagt gattgcgtat
     2521 taaatgaaat caagtaacca acaggtgttt taaataaaat aataattaag tttaataata
     2581 gtattccaat gaaaaatttg aaaaatgttt tttgaccatt tttcattgct ttaaatccat
     2641 tagctaagtg tgtttttact ttatcgttag ttgattcaac gaaactagtc ataaagttga
     2701 aaataggtat taacaataac caagtaataa ttgctttaat caataaaaca acaatgatta
     2761 tgatgctttg agttgtaata gcaataccaa tcaggtgtgt actatctgca tatgatgatt
     2821 gtactgaatt cataatcatt tctgatactg gagaaaataa tttgtatagt aatgatgtca
     2881 gaagtgaaag aacgataatc attgctatag atacaagacc gatcagaacg ctctttaaat
     2941 aattaccttt tttaaatgca ataaataaat cggtaaattt tactttttca tgactcattg
     3001 ctcgtttcat aacatttgta attccgataa aaatttgaac taaaacaaac agtgaaacta
     3061 cagctgctat aagtaataca gcgataactt ttaaatatgc atctactggt ggtttttgcc
     3121 caaattgtga ataaattgca atagtttgtg catacttagc aagctggaaa ttagcaagta
     3181 aatatacaac tgcaattaca gcaaatgcta taagtgcata tatcaatgtt attaataatt
     3241 gtggtttagc atttttaaat gctgatttaa agtaagtaaa caaagtggtg cctccttttc
     3301 ttcatataaa tacaataaca aaaaacacgt tattttcata ttaatatcta ataaaaatta
     3361 attaaagtga ctaaagttag agaattataa tagtaaattg tcaaaaatat gactaatgta
     3421 agaaataata gttgaaaatt tgttaataaa cattgcatca tcaaagacca aacaagtcgc
     3481 cataaagaca taaaaaacct actgccattg aaaagtttaa tcgatgcaat ggcagtaggt
     3541 taaaattatt aatgtacatg cgcgtgatga tgagcatgat ggttatgtga atgactatgt
     3601 gttcctgaac atataatcga ttcatcatgt ttgtgattag gcgtttctaa ttgaatagtc
     3661 atatggtgaa tatttaaatg taataaatca tgctcaatgt tttctaataa taattcacat
     3721 tctttcattg tcaatgtatg gtctacaaca acatgacaac tcaatgcatt catatcattt
     3781 gaaattgtcc aaacatggca atcatgcaca ctttgtattc gtgaatcctt tttaatagta
     3841 gttataactt catctatatc aacatcactt ggtgtgcctt ccattaaaat gttaattgaa
     3901 gattttgtga taccccaagc actttttaaa ataataacgg aaactaaaat acttgcgata
     3961 ggatcggcga ttgtccatcc aaatgcccaa attaaaatag ctgcagtaat ggcgccaact
     4021 gaacctaata agtctccgat aacatgtaga aaagcaccac gcatatttaa attgtgtgaa
     4081 gtgtcgccgc ctttaaacat aaagaatgca acaacgatat tgacaattaa accgataata
     4141 ctaatgatta acatttcttt tgattgaact tcagaaggaa caaagaaacg tttaatcgct
     4201 tcaaaaacaa tcaaaatact tattacaaaa agcgttacac cgttaaataa cgctgcgagt
     4261 acttcgaaac gtttataacc aaatgttttt gtagttgtgg cattcttttc agcataaata
     4321 aatgcgacaa gtgcaacacc taatgagaat gtgtcgctaa acatatggat accgtcagat
     4381 agtaatgcca agctgttagc aaggagaccg ccgatgattt cgataaacat atatagaccg
     4441 attattaaaa acgatataaa caatactttc ttattatttg tagttacatg actatgcata
     4501 tggtcatgat gatgatgtga atgagacata ttataaccca ctttctttag gatgattcgc
     4561 gtgatgtatg gcttgcttta acatagttgc tacgtggatg tcatctaatg aataaatcat
     4621 tgattggcct tgtcgttttg ctttcacaag atgcacactt ttaagtaatt ttaattggtg
     4681 cgagacattt gattgagata aattcaattg atgtgaaatg tgaccaacac ttgcttcgct
     4741 gactgataac aattccatga tacgtattcg attgtaatcg cctaatgcct tgaaaatttc
     4801 agttacgcgt tctaatgtat ctgtatttat ttctgaatat tgttctgaca ttatatgaca
     4861 atcctttcat gtgaatattt gttcatatat taatttaata cgttcccagt agtatgtcaa
     4921 ttgattttta tagttgtaac aagttttaca tatctcataa agtggtatgg catagagaat
     4981 aataaatagg agagtgttgt aaatgaatat tttggttata ggtgctaatg gcggtgtagg
     5041 ttcgttacta gtgcaacaat tagcaaaaga aaatgtacca tttactgctg gtgttaggca
     5101 atcagatcaa cttaatgcgt taaaatcaca aggtatgaaa gcaattcttg ttgatgttga
     5161 aaatgattca atagagactt taactgagac gtttaaacca ttcgataaag ttatcttttc
     5221 agtaggttct ggagggaaca cgggtgcaga taaaacaatt attgtcgatt tagatggtgc
     5281 tgttaaatca atgattgcga gtaaagaggc caatattaaa cactatgtca tggtttcaac
     5341 atatgattcg agacgtcagg cttttgatga tagcggtgat ttaaagccat atacaatagc
     5401 taaacattac gctgatgact atttaagacg ttcaggtttg aattatacaa ttttacatcc
     5461 aggggcactt acaaatgcag caggatctgg aaagatagaa gctgcacaat attttgatgg
     5521 taaaggtgaa attcctagag aagatgttgc gacagtatta aaagaaattg tcacatccaa
     5581 tcattttaat caccaagagt ttcaaattat tagtggtgat caggacatta aagatgcttt
     5641 aactcaattt gaaaatgaaa cggattaata aggatagtaa atatgatttg aagcgggtta
     5701 ctttttcgta actcgttatt ttttgaaaat gatgaattat aagtatgaat aagtacgtat
     5761 tgtctatgcg ttttacaaag aatttgaaca atgtatggca caactattaa agttgtttat
     5821 taattggtat aatgggcgag taaatttaaa gggaaatatg tacaaatgta agagagatat
     5881 aatctctatg ctataaattt ttgaaaatgt taaagtactg taaatttaag tgaagcgctt
     5941 taattggcag tactgcaatg ttagtaaata tggtacaatc actaatagtt aatttgaaat
     6001 gtggaagtta ataatacgat gtaaatgaga acaaaatcaa aatataatgg aacaacaaca
     6061 tcagagttct attgcttact taatcacaat ggaggtgcaa caatgccatt atttttacaa
     6121 ccaattttaa aaacaaaatt atggggcggt caacgtctaa gtgagtttgg atatcaatta
     6181 gacaatgata caactgggga atgttggtgt gtgtcagcac atccaaatgg tacgagcgag
     6241 attattaatg gaccatatca aggtcaaaca ttagaccgta tttggtcaga acatcgtgaa
     6301 ttgtttggtg atttcccaag caaagatttt ccgcttctaa ctaaaatagt ggatgcaaga
     6361 gaatcacttt ctattcatgt gcaccctgat aattcttatg cttatgagca tgaaaacggg
     6421 caatatggca aatctgaatg ttggtatatt atagatgcag aagaagatgc agaaatagtt
     6481 atagggacat tagcagagtc tagagaagaa gttgcgaatc atgttcaaca cggaacgata
     6541 gagtcgatac ttagatatat taaagtaaaa cctggagaat tctattttat tccagcagga
     6601 acagtacata ctatttcttc aggaatatta gcatacgaaa cgatgcaatc gtcagacatt
     6661 acatatagac tttatgattt caatcgtcaa gataatcaat ataatgatag accgttaaat
     6721 attgaaaaag ctttagacgt tattcagtac aatgcaccat tacctaatat tttgcctgaa
     6781 agcgaaatta ttgaaaacca taagtgtaca cacattgtat cgaatgattt ctttacattg
     6841 gttaaatggg aaatttctgg cacgttaaat tatatgaagc ctagagagtt ctgtttagtt
     6901 acagtgttgg aaggcgaagg gcaaatgatt gtcgatggtg aaattttcaa actgactact
     6961 ggtacaaact ttattttgac ttctgaagat ttggatagtg tctttgaagg tgatttcaca
     7021 ttgatgatta gctatgtgta atagtgtaat aacaatttag gagtgaatga tttgaagcaa
     7081 tttctatata ttgcgttagt atgtggtgtg atagcaggtc ttggtgcttt cttacatata
     7141 ccgcagtatc cgagcatgac aattccacgt atagtagcta ttttaggaat tatcagtgct
     7201 atgttgactt ttaaagacaa gcaaatcagc gcctcattaa agtttagcgc attgttaatt
     7261 aatgtgctgc cattatgcgg tacctttgta gcttcaaatt aattgtcgat agcgcgtttg
     7321 aatataatac agtgcattaa cagaagaaat atgattgtgt taatgacatt gatgaaaata
     7381 aagcacatca acataacctc tcacttgcat agtagtgagg gggtttttgt tggtttatat
     7441 aaaatgaagt aattaatatt taatgaaaat taagacatga taaaattcta acggcagtat
     7501 agatatttaa aaatgtgtat caatggcgct atagagataa gcaagcatag tggtatcata
     7561 tataaatttg taatttttat tgcatagtgt atgcaagcat gttttttata tgcaaaatag
     7621 gtagctaaat atgttaaact gtttgtaaca atttttatca ttaagacaga tataataaat
     7681 aaaatataga aaactgatta aaaggtatac agttgctcgt atgtgtgtcc aactgtattt
     7741 agagtcgcac ttgttgtaaa gcaaaatagt tcgacttact tagaagtgaa aacgataagg
     7801 gtctgggacg taaattaatg tcctgggcgc tacaatgtta taggaaatgc gattaaaatc
     7861 gcagtgaaca aagggtatac agttgttcgc atacgcgtcc aactgcataa gaaacactaa
     7921 aagaatgtaa ttcatagtgt ttctaaaatt tattcaggtt aaggagtatt aagatgacag
     7981 cagaaacgaa ttatttttgg ttgaattgtg gatataaccg ttggaatcat aatgaaccgt
     8041 tagtaggaca aacagcttta tttgaatcag gcgcacattt taacccatct caaggattta
     8101 gagcgtttaa aaaggctaaa gtaggagatc aagtcatctt ttatcaagtt cagacggata
     8161 ctggactact tggatgtggt gaaattatta gtgttgaaac aggtgcacaa aataaaatac
     8221 gtgtacagtt tcgttttaat gaacaattga aaccattaac agcagattat ttaaagcgta
     8281 gtgaagcttt ggaatttcgt ataagtaata tgaaagagac attgttcaat caaatcactg
     8341 cagaagagtt tgatttaata agtggtcttg gtaaaggcga aattaagata ccgcgttatt
     8401 tctttttagc tgaaactgaa gaatttgaac ctggaaacca atatacgatt tatacgcaca
     8461 cgtacaacgg tattaaacgt aatggttatc atttttatac tcagttagaa gaaggcgaca
     8521 atattatttt ttataatcga acgaaaaatc aatctgtcgt tggaattgga gaagtatcaa
     8581 agcatattca tgaaaaacca ccaattcctg gtagaacaaa tagtacagtg attgaagtgt
     8641 cttatgagaa agacattaca ccgattacat taagtacatt aaataagcat cctaaattga
     8701 aaaacttata tttccttcaa gaaaatgcaa aacaggctat tgcaagtatg tcacaagcac
     8761 agtatgatgc aattattgaa atgagcgata ataatggatt gaaatctcca tttgaaatgg
     8821 ttcaaaaacc agatatgctt gaaagtgaaa aagaagaagc gttaaagcca tttattttac
     8881 ttgttgtaga tcgaaaagaa gaaggcttaa aagcggctaa tgacttattg caaaaagcga
     8941 atgcaaatcc tgttataaca acaggtcatc ctgatttttc agaagatatg ctttatggca
     9001 agtatttacc aaatgaaaca ggggcattat actatcgtga agggtttatt actcaattaa
     9061 tgcctaaaaa ggataaaagt tatttagtta ttgataattt taatcgtata gatacagaca
     9121 ttttccaaac gtatattaat gtgttagaag gctatgaagt aacattacca cgttataata
     9181 aagacggtaa tatgattaaa tggtcacgac aaaaagattc tttctattat ttcaatccta
     9241 attggcatat tgtaggtatt acatatgaca gtttagagga aattaaggag aaatattcag
     9301 agcagttttt aaaatacaca cgtatagtca aagtcaaaca tgactaatga tatatggtag
     9361 tttttgaaaa gaagtaagga taggattaaa aattgcataa ttgcatattt gaataaccgc
     9421 ataaagaaaa aatgacgggt aatcaaccat aagagctaat taagagctta tgatagatta
     9481 ctcgttttta ttttaataaa taataaagac agtatatgaa tataaaagaa tttatgttac
     9541 agaattaaaa ctttatatga gttttccagt ggtatgttga ataagacgaa tgaccaaacg
     9601 ctaagttaat aataagaaat aagattacac tgtagattga gaaatttaaa gtaaataaaa
     9661 aatacattat acaataaata taaaactgaa aaataaattt tatggcaatt tcgtgtcgcg
     9721 aggacttatc aagtgacaac cttgtttaaa tgtttttaaa ttaggaaaaa tgactataag
     9781 tactattaaa ggagcgaata aattatgcca atcgtatatt atgatggaaa ctgtatatat
     9841 tgttataact atgtcatttg gttaattcaa catgacctac caagaaatta tcaatttgcc
     9901 acgttaaaag gtgaagttgg ccaacaattt tttaaacaac atccagaagc ggcaaataaa
     9961 aatagtgtga ttttacagaa aggtgatcgt atatattttg aatcgcaggc gattattaaa
    10021 ctaatcacag cactacctaa tcgaactaaa ttattgggcg taggtttatg gcttgtacct
    10081 aaaccagtcc gtaatttcgg ttatcgaatg tttgctaata atagaaatag aatgtggaaa
    10141 acaacatggc accaaccaaa tgattatgaa aaatcgttct ttttagatga taatgcgaaa
    10201 gtaaaactta ctgattgata aaacatactt gctaattgat aatggatata ttagatgatg
    10261 aattaaaatt taggcattta aatagcggaa cactttacgg ttagattaga ataattataa
    10321 aaaagagagc aaaaacactt tacagattag aattattata atataataat taatataaac
    10381 aagcaagacg tagacaattt taaggagtgt attaaatatg agtaatcaac aagatgttgt
    10441 aaaagaattg aatcaacaag tagcaaactg gacagtagct tacacaaagc tacacaattt
    10501 ccactggtat gtgaaagggc ctaacttctt ctcattacac gttaaatttg aagaattata
    10561 taatgaagca agccaatatg tagacgaatt agctgaaaga attttagcgg taggaggaaa
    10621 ccctgtaggt acattaactg aatgcttaga gcaatcaatt gttaaagaag ctgcgaaagg
    10681 ttactctgca gaacaaatgg ttgaagaatt atcacaagac ttcacaaata tctcaaaaca
    10741 attagaaaac gcaatcgaaa ttgctggtaa tgctggcgat gatgtatcag aagatatgtt
    10801 tataggtatg caaacatcag tagataaaca taattggatg tttaaatctt acttaagcta
    10861 atctacagat aagtaataat ataaataaaa gccaacacat aaatggggat acgcccagaa
    10921 tgtgttggct ttttgagttt aatcgcattg ttaaagagca attaaagtca atttaaataa
    10981 gttactctgc gatttctaaa gcgatttcca tcatttgtgt aaatgaattt tgacgttctt
    11041 caggtgtagt agcttcgtca cgtaaaatat gatcacttac tgtgaaaata ccaagtgctt
    11101 ttttaccagc atgaatcgca tttaaatata aaccagctga ttccatttcg atacctaaaa
    11161 tacccatttt tttccaagca tcattgaatg ttggatcggc attgtaaaat gtatcagaag
    11221 ataatacgtt acctacgtgt gtagtagcac cgatttggtc agcgacattt ttagctttag
    11281 ttactaactc gaagtcagcg ataggcgcga aatgacctgg aatattgtat tgatctacat
    11341 aatttgaatt agttgatgca gcttgtgcaa taataacatc gtataagtta acattttctt
    11401 gtaatgcgcc acaagaaccg atacgaatga ttgtatctac atcaaagaag ttgtataact
    11461 catatgagta aataccaata cttggaatac ccataccaga acccattaca gaaacttctt
    11521 tacctttata tgtaccagtg taaccaaaca tgttacgtac atcgttaaat tgttcaacat
    11581 tttctaaaaa attatcagca atatattttg cacgtagcgg atcgccaggc attaatactg
    11641 ttttagcaat ttttactcca tttggttgaa tatgtggtgt accttttgtc atttgttatc
    11701 ttctccttaa tattcattaa taattttaag ataacatttg tttccctttt ttgcgaattt
    11761 ttgtactata gaattaaaga attataaaaa tataatcctg attagcttgt tgtcaagtca
    11821 tcgttcataa tgaagatatc acgttcaatt gtattgtttg tttatgggaa atgaattaat
    11881 gtaatagtat atgtatgcgg ttacataaaa agcgaacatc taacctgata tttaaatgaa
    11941 cctgacgctc aatcaactaa tttacaaccg tatttttata atcaaccata aaggaggaga
    12001 tagaaaatga atagtgcaaa attgattgat cacactttat tgaagcctga gtcaacacgt
    12061 acgcaaatcg atcaaatcat cgatgaagcg aaagcatacc attttaaatc tgtatgtgtg
    12121 aatccaacgc atgttaaata tgcagcagag cgactagctg attcagaggt gttagtttgt
    12181 acggtaatag gattcccatt aggtgcatcg acaactgcga cgaaagcatt tgaaacagaa
    12241 gatgcgattc aaaatggtgc agatgaaatt gacatggtca tcaacatcgg cgcattaaaa
    12301 gatggacgtt ttgatgatgt acaacaagac attgaagcag tggt
//
LOCUS       DAKTGS010000083        12234 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.083, whole genome shotgun sequence.
ACCESSION   DAKTGS010000083 DAKTGS010000000
VERSION     DAKTGS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.083"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..143)
                     /locus_tag="O0286_001830"
     CDS             complement(<1..143)
                     /locus_tag="O0286_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCY0170206.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVIKNTKVIILKNKIFVFEQ
                     SIK"
     gene            294..1292
                     /locus_tag="O0286_001831"
     CDS             294..1292
                     /locus_tag="O0286_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0170207.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1524..2405
                     /gene="rbsU"
                     /locus_tag="O0286_001832"
     CDS             1524..2405
                     /gene="rbsU"
                     /locus_tag="O0286_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCY0170208.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2420..2824
                     /gene="rbsD"
                     /locus_tag="O0286_001833"
     CDS             2420..2824
                     /gene="rbsD"
                     /locus_tag="O0286_001833"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCY0170209.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2852..3766
                     /gene="rbsK"
                     /locus_tag="O0286_001834"
     CDS             2852..3766
                     /gene="rbsK"
                     /locus_tag="O0286_001834"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCY0170210.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3864..3998)
                     /locus_tag="O0286_001835"
     CDS             complement(3864..3998)
                     /locus_tag="O0286_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170211.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            4017..4778
                     /locus_tag="O0286_001836"
     CDS             4017..4778
                     /locus_tag="O0286_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0170212.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5212..6648)
                     /locus_tag="O0286_001837"
     CDS             complement(5212..6648)
                     /locus_tag="O0286_001837"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCY0170213.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6664..7455)
                     /locus_tag="O0286_001838"
     CDS             complement(6664..7455)
                     /locus_tag="O0286_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0170214.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7604..8308
                     /locus_tag="O0286_001839"
     CDS             7604..8308
                     /locus_tag="O0286_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0170215.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8416..9117)
                     /gene="lrgB"
                     /locus_tag="O0286_001840"
     CDS             complement(8416..9117)
                     /gene="lrgB"
                     /locus_tag="O0286_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCY0170216.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9110..9553)
                     /gene="lrgA"
                     /locus_tag="O0286_001841"
     CDS             complement(9110..9553)
                     /gene="lrgA"
                     /locus_tag="O0286_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCY0170217.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9666..10406)
                     /locus_tag="O0286_001842"
     CDS             complement(9666..10406)
                     /locus_tag="O0286_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCY0170218.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10409..12163)
                     /locus_tag="O0286_001843"
     CDS             complement(10409..12163)
                     /locus_tag="O0286_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0170219.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
BASE COUNT         3973 a         2298 c         1949 g         4014 t
ORIGIN      
        1 tttttaatac tttgttcaaa aacaaatatt ttattcttta aaataatgac ttttgtattt
       61 ttaatcacaa taaacatttt aaaattcttg ttatcataat cattaaaagg tattaacctt
      121 aataatatat tctctcgtct caaccttaat cgtatacttc agacgtctgt ttgtagacaa
      181 taaaagtcat tcacgtcttc atatgtcatc aaatgtttat catgatatga tgaatataat
      241 aatcgggtat ataactgtat gattaattac acaataaaga gaaggaatat aatatgaaaa
      301 aagtgtcaat taaagatgtt gctagagaag ctggtgtatc agttacaact gtgtcacata
      361 ttttaaatca taatgatagt cgtttttccg caacaacgat aaaaaacgta catgctgttt
      421 cagaacgttt aggctatgcc cctaataaac atgcaaaaca attgcgcggc agtaaaattc
      481 aaactattgg cgtcattttg cctagcttaa caaatccgtt tttctcagca ctgatgcaaa
      541 gtattcatga ccataaacca tctgatgttg atttatgctt tttaacatct acagcaactg
      601 atttgtatga caatattaaa catttaattg atcgaggtat tgacggatta attatcgcac
      661 aatacatatc atccccggac gccctaaata actatctaaa gaaacatcat gtaccttatg
      721 tcgtactgga tcaaaatgac catcaaggct atacagattt tgttcggaca aatgaatatc
      781 aaggtggaca acttgcagca caacatttaa tagaactcgg tcacaaccat atgataattg
      841 ttgcaccata tgacatgatg gcgaatatgt cgactcgtgt cgctggattt gtcgatactt
      901 tgcgcgcgaa tcaattgcca gaaccacaaa tcgtccatac tgaattatct aagcgcggtg
      961 ggctaaccat tgttgatgac atcatggttc aatctgccac tgcaatcttc gctattaacg
     1021 atgaactcgc tattggcatt ttacgaggac taattgaaca tggcatcagt atcccgaaag
     1081 atatctcatt aataggttat gacgacattg attatgcagc gtacgtctcg ccacctttaa
     1141 ctactgtggc acaacctata actgatattg gcaaaacatc tttaacctta ttacttcaac
     1201 gattacagca cttagataaa tccattgata tgattgaatt accaacgact ttaaaaattc
     1261 gtgcaacaac tggctatcat ctttcaaact aactacgtat cttccgaaat atactcatca
     1321 ttgttaggcc cttagcgttg ctttaatgct gagggttttt aatcataatt attttactaa
     1381 gaaattaaaa taataatgta tgaattttta aatatgattt aaacgttttc agtttttatg
     1441 aaaacgcatg cattttacaa ataaaaatgg tacgatggca ctggtaaaac gttttactaa
     1501 aaacaaatca tgaggtgtat aacatgagca ttgttgcatt acttatcggg ttaggcccct
     1561 taattggctg gggcttcttc ccaacagtcg cttcaaagtt tggtggtaaa cctgtacatc
     1621 aaattatcgg tgctactgta ggtacgttaa tcttcgctat tatattagcc gtagtcacat
     1681 caagtggctt acctactgga accaatttgc tattcgcctt attatcaggt gcaggatggg
     1741 gattcggaca aatcattaca tttaaagcgt tcgaattagt cggctcatct cgtgccatgc
     1801 cagtcacaac agcattccaa ttattaggcg catctttatg gggtgtcttt gcattaggaa
     1861 attggccagg cattggtcat aaaatcattg gatttacagc tttagtcgtt attctaattg
     1921 gagcgcgtat gacagtttgg agtgaacgca aagaagcaag taacgccaaa aatttacgtc
     1981 gtgcagtggt acttctgtta attggtgaat ttggatactg gttatattca gctgcaccgc
     2041 aagcaacttc tattgatggc ctaactgcct ttttacctca agcaatgggt atggtaattg
     2101 ttgcagtcat ttatggcttt atgaatatga aagcagagaa tccattccgt aataaaatta
     2161 cgtggttaca aattatttca ggtttcttct ttgcatttgg tgctttaaca tatcttattt
     2221 cagcacaacc taatatgaat ggtttagcaa ctggatttat tctttctcaa acatccgttg
     2281 tgcttgctac attaactggt atttatttct taaaacaaca taaaacgtca aaagaaatgg
     2341 ttattacaat catcggctta gtactcattt tagtagccgc ttctgttaca gtatttataa
     2401 aataaggagt gtagatgtca tgaaaaaatc agctatttta aatgaacata tttcaaaagc
     2461 aatcgcgaca attggtcatt ttgatttatt aacgattaat gacgctggca tgccaattcc
     2521 aaatgatcat cgtcgtatcg acctagctgt aactaaaaac ttaccacgct ttattgatgt
     2581 cttagctaca gtgttagaag aaatggaaat ccaaaaaata tacttagcag aagaaataaa
     2641 agaacataac cctacacaat tgcaacaaat taaacaattg atttcatcgg aaatcgaaat
     2701 cattttcatt cctcacgaag aaatgaaaag taacttagct cacccattaa ataaaggtaa
     2761 tattcgtact ggtgaaacaa cgccctactc taatattgca ttagaatcga atgttacttt
     2821 ttaaaagtta taacttgaaa ggagcgtaca catgaccaac aaagttgtta ttttaggttc
     2881 aacgaatgtc gatcaatttt taacagttga aagatatgca caaccaggcg aaacattaca
     2941 tgttgaagaa gcacaaaaag cattcggcgg aggtaaaggt gccaaccagg ctattgccac
     3001 tgcacgcatg caagcagaca caacatttat tactaaaatt ggcactgatg gcgttgctga
     3061 tttcatctta gaagatttta aagtagctca tattgataca tcatatatta tcaaaacagc
     3121 tgaagcaaaa acgggccaag cctttatcac tgtgaatgca gaaggacaaa acaccatcta
     3181 tgtttatggt ggtgcgaata tgacgatgac acctgaagat gttattaacg caaaagacgc
     3241 tataatcaat gcagactttg tcgttgcaca attagaagta cccatcccgg ctattatatc
     3301 tgcatttgaa attgccaagg cacatggtgt gacgacagta ttaaatcctg caccagcgaa
     3361 agcattacct aatgaattat tatcattaat cgatattatt gtgccaaacg aaacagaagc
     3421 cgaattgtta tctgggatta aagtaactaa tgaacaatct atgaaagaca atgccaatta
     3481 ctttttatct ataggcatta agactgtttt gattacgcta ggtaagcaag gtacatattt
     3541 tgctactaaa aatcaaagcc aacacatcga agcttataaa gtaaatgcga ttgatacaac
     3601 tgctgcaggc gacacattta ttggtgcatt tgtcagtcgc ttaaacaagt cgcaagataa
     3661 cttagcagat gctattgatt ttggtaataa agcgagctca ctcactgtac aaaaacacgg
     3721 cgcgcaagca tctattcctc tactagaaga agtaaatcaa gtttaaatga atcaaacaca
     3781 gctatgatat gaaggtttag catataacat gcaacattcg tatatcatgg ctgtgctttt
     3841 ttatctttat aaaacatcat ctattagaaa taattttcca ctaaacctat gcttgttgac
     3901 tcatgtttag ttataaatga agtgacaatt ttttgtaatc tttttaactt ccaaattatc
     3961 gcatataaat atgctatatt aatgataata attatcaatt aaaaggaggt tatgctatgt
     4021 ctaaagaagc tggtcataca tttttagcta aattaggaaa aactcgtcta cgccccggtg
     4081 gtaaagaagc aacagattgg ttaatacaac aaggggcatt ttcacaagat aaacaagtgt
     4141 tagaagtggc atgtaatatg tgcacaacat ctatttatct agctcataca tatggctgtc
     4201 acattcaagg cgttgatata aataagaaag cattagaaaa agcacaggaa aacatttcag
     4261 cagcaggtct tgaatcatat attcaagttc aacaagcgaa tgctgttaaa ttgccctttg
     4321 atgacaatca attcgatatc gttttaaatg aagcaatgtt aacaatgtta cccatcgcca
     4381 taaaggaaaa agcattacgc gagtactacc gagtcttaaa gcctgggggt atcttgttaa
     4441 cacatgatat tgtcatcgtt aatgaatcac atgccacaca tgttgttaaa tcattatctg
     4501 cagcaattaa tgtcaatgtc tcaccgcaga cgaaacttgg ctggttagat ttatataatc
     4561 aagctggttt taatcatgtg cattatcata ctggtccaat gagtttaatg acaccaaaag
     4621 gtttaattta tgacgaaggt attgttggaa ctataaagat tatcaacaat gctttgaaaa
     4681 aagaaaatcg accaatgttt tgtaaaatgt ttaaaacgat gactaaattg cgtaaagata
     4741 tgaattatat tacttttgtc gctaaaaaag agcactaaat ataatgccac taactgtact
     4801 ttgtatctat gtttgactat cactttaatt tctttgtgac actaatcatc tacttaacaa
     4861 tatcgttatc gttgattagt aagtcatcaa ttttggttaa agactttcat aaacactcaa
     4921 acattaacac tatacatagt tagtggcatt atttttttct taaaatttta acatcacggg
     4981 attggaacag aaatgatatt ttcgcaaaat ttatttctgt ccttccccaa cttgcattac
     5041 ctgtagaatt tcttttcgaa tttctctgtg ttggggcccc gccaacttgc attacctgta
     5101 gaatttcttt tcgaaattct ttgtgttggg ggcccgccat tataacactg taggtcctac
     5161 tatattaatt tatgtctcgg gctctaaccc taaaatactt ataatatacc tttataattt
     5221 atctccattt gatgctatca cttgttgata ccaatagaac gaatcttttt tcaagcgttc
     5281 catcgtgcca tgaccatcat tatctcgatc aacatatatt aaaccatagc gtttcttcat
     5341 ttcaccggtt gtaaatgaaa caatatcaat gattccccac ggtgtataac cgattaaatc
     5401 tacaccatct tgatcaactg cttctatcgc tgctgtaata tgtgctttta aatattcaat
     5461 gcgataatca tcatgaatat gtccatctac cacttcatca actgcaccaa aaccattttc
     5521 cacaataaaa agtggtaact gataacgatc atataacaca ttcaatgtat atcttaagcc
     5581 atctggatca atcgcccaac cccaatcact cgttgcgata tgcggattct ccacagaatg
     5641 attcaaacca ccgttgacga tgttgttttc aactgtagta tcaacatcat gttttacagc
     5701 cgtagacatg taataactaa agccaatata atcaaccgtg ccctgctgta aaatactgtc
     5761 gtccccttct tgccatccaa catcatatcc ttttcgtgcc aacatttttt tagcatagct
     5821 tggataataa cctctcactt ggacatccgg gaaaaagaaa cgtaagcgat tcgcaatttg
     5881 tgcttccatc atatctttcg gatgacacga atatggataa atgggtacat gtgaaatcat
     5941 tgttccaatc ttaaacttcg gattaatctc tttaccaaga cgaactgcta aagcactggc
     6001 taaaagttca tgatgtgcta cttgatacaa gacttcttca ggattatcat tttctgtcaa
     6061 tgctacccca gaattcgtcc ataaaaagat aggatttgat gtgtccatct gattattaat
     6121 ttcattaaac gtcatccaat atgtaacttt atctttatat ctttcaaata caacacgcgc
     6181 aaaatgcaca aaataatcga caacttctct atttctaaat ccaccataat gtttcgctaa
     6241 atgaagtggc atctcaaaat gtgataacgt cacaacaggt tcaatacctt gtgcaattaa
     6301 ttcatcaaag atacgatcat aaaaggcgag tccttcttca tttggcacat cttcatcccc
     6361 attcggaaag atacgtgtcc acgcaatcga cgtacgtaaa catttcaatc ccatttcttt
     6421 aaacaaggca atatcttcct tataacgatg ataaaaatca ataccttcat gatttggata
     6481 atagtgattg ggatctatag attctgtaat ctgacgtgct ttgccatgtg caccactcgt
     6541 cataacatca attacactta accctttacc acctttatca tatccacctt caaattgatt
     6601 tgcggcaaga gcgccacccc acatgaaatt ttgcggtaat tttgtcatta ttacaacaca
     6661 ctcctagttt ttaaatattt taaaaataac atcttcagtc gtaatgattt cattcattgt
     6721 cagttctact tttcctaaat cggcagagtt gctaataacg acaataacgt cagcattgta
     6781 gccttgttgt tgtatatatt gctggtcgaa ttgcaacaac gtttgccctg cttcaacatg
     6841 atcattttgc tttacaaagc acttgaaccc tttaccttcc aagtcaactg tattcaagcc
     6901 aatatgaatg actatgtcca caccgtcttc tgattgaata ccaactgcat gcttagttgg
     6961 tacaatcata gatattaaac cattgaacgg tgcgattact ttggattctt catgagcttt
     7021 gattgctaag ccttcaccta ccattttctc tctaaaaatt gaatctctta cttctttaag
     7081 taaaatacga cgaccagcga ttggtgcttt cactaaaatt ggtgttgtta cttttacttg
     7141 aggtgtataa cttgtatctt gactattaat attgtcagta ttctcactct cttcgacatt
     7201 agcattctta attaatcgct gtaattgctg atacacttca tcgacatttc catttaattt
     7261 tatgactaac tgcgtatcat tttctgctac agatgtcaca tcaactattt gtttcacttc
     7321 atttgctgta gaaggaattg tatgttgcat atgaattgta atgcacttcg tatcatacgt
     7381 tgcatcaaca atgttttcga caccacctac agctgtaaga atgtcttggg cttgttcttt
     7441 atatttattg ctcataaata ttaccccctc aacttgatag tagaatgaaa atgtatttac
     7501 ttacctccaa ttataattgt accggttcaa tttgtaaacg ccgatacaat tataatattt
     7561 tgtgctataa taattacaga caaagtgaaa cgaggacaga atattgttaa agtatgaaca
     7621 tattgctaag caacttaatg cgtttataca tcaatctaat ttcaaacccg gtgataaatt
     7681 gccaagcgtg acgcaattaa aagaacgtta tcaagtaagt aagagtacta tcattaaagc
     7741 attaggctta ttggaacaag atggtttgat ctatcaagca caaggcagtg gtatttatgt
     7801 gagaaatatt gctgatgcca atcgtatcaa cgtctttaag actaatggtt tctctaaaag
     7861 tttaggtgaa caccgaatga caagtaaggt acttgttttt aaggagattg caacgccacc
     7921 taaatctgta caagatgagc tccaattaaa tgcagatgat accgtctact atttagagcg
     7981 attaagattc gtggacgatg atgttttatg tatcgaatat tcttattatc ataaagaaat
     8041 cgtgaaatat ttaaatgatg atattgctaa gggctctatc ttcgactatt tagaatcaaa
     8101 catgaaactt cgtattggtt tttcagatat tttctttaat gtagatcaac tcacttcaag
     8161 tgaagcttca ttactacaat tgtctacagg tgaaccatgt ttacgttacc accagacttt
     8221 ttatacaatg actggcaaac cctttgattc atctgacatc gtatttcatt atcgtcatgc
     8281 acagttttat attcctagta aaaagtaata aatacataaa aacgtctata tcccagttat
     8341 aaactggagt atagacgttt ttttacgata ataacaatgg ctcaaattgc tattatcttg
     8401 cttaggtttt tcgttttaga agaatattgc tacaaagaca ggcacaactg ctacaacaac
     8461 tacaccaact aacactaaag ctatacttgc cattgattct tctacaggtc ctaattcttt
     8521 ggctggtgct acacctaatg tgtgaccact tgttccaagt gctaatcctc gggcaatagg
     8581 gttagtaatt cggaaaagct ttaagaattt attacctagg gcataaataa tgacaccatt
     8641 taaaataact gctaatgatg ttaattcttt tataccaccg ataccagctg atactggtaa
     8701 cgcaatcgct gtagttgctg cttgaggtaa cattgataaa ataacatcat tggcaaattg
     8761 tgctaacttc gcaaaagtta aaataattaa taacgctaca actgtaccga taccaatacc
     8821 tccgatgata cgatgccaat gtttaacaag cacttcacgc tttttatata acggaatcgc
     8881 aaaacagatt gttgccggtt ctaagaagaa gtaaataatg tctccaccta ttttgtaagt
     8941 cttatacgga atgcctgtta aatagaggaa ggccacacca aataccatac tgacaaatag
     9001 cggtgcgaat aagaagaaac gattagtttt ttcaaataat atggtcgcta agaaaaatgg
     9061 tataacggat aacagtattc cgaagtaagg tgtgtttagt gctaagtggt taatcatgag
     9121 cttgtgcctc ctctattttg atcttttttg tgactttgtc acctttagat ctcgaagtaa
     9181 ctttcataat aatttgtgtg acatagccag tacaaataag taatagtatt gttgagacga
     9241 ttattagtcc aatgattaaa aatggtgctt ggctaatgac acctaaagag ttaacaactg
     9301 agataccggc tggtacgaag agtaagccaa tgttatttgt tagtgtcgtt cctacttttt
     9361 cgacttcgcc taacttaaca gcaccagtac ataataatac aaataataat actaaaccga
     9421 ttactgatgc aggcatagga attggcataa atgattcaat tattttcgat acaaagagta
     9481 ctaaagcaat tacaatgact tggtgaaaaa agtgtgctgg ttttgatgcg tctttttgtt
     9541 gtttcacgac cattgcctcc tacgtttgat ttaactaaag tatagatggc tcacttcgat
     9601 ttgcgtgatt tttagtccga aatacaaaat atcataggta aaatgcataa aaaaaaggat
     9661 tactgttaaa gtaatcctat cgacgcttta aaatctttca taaatgaacg tccaacttgc
     9721 atcttgacac catttgtcaa tattaccata taagtatagt taaaccattg ttgcacttct
     9781 ttaatgtgtt tcgtgttaat aatatatgaa cgatgaatac gtataaaata agtgggattc
     9841 aatcgttttt cataacgatt caatggctct gttgtttcgt atttatgatt cgttgtatgt
     9901 atggttgtaa taccattatg tgtgccaatc ccaataatat tttgttgctt taacatgtga
     9961 attttatcgt caatttcaac aggtaagctt tgatcaaaat tcgccgacat atcattcgca
    10021 attgcacttg cgttattatc atctttggct ttagtcgcac gcactttatt gactgcttgt
    10081 tcaatacgtt tttgaccaaa cggtttcaaa atatagtctg tcgcatttaa ttcaaatgcc
    10141 tgtactgcgt attggtcatg tgcagttgca aaaataatcg caggtggctc tttcatcttt
    10201 tgaatcttag ctcctaattc gatcccattt tcatccatta aattgacatc taaaaatata
    10261 atgtcatatt gattgatcag tagtgcttcc aatgtttctt ttacattttc tgcctcatta
    10321 atttcttcaa aaccaccaat ttcatttaat aaatatgtta attcattacg tgctaatggc
    10381 tcatcatcta tgattaatgc tttcatattt attcctcctc ttgtctttca taaggaagta
    10441 cacaccaaaa agtggtaccg ctcgatgtcg attcaaattg taatgctgcg gattttccaa
    10501 ataatccttt taggcgtaag tttaaatttt ctaaagcact accagttcca gactctgatt
    10561 ctacagatgt ttctcccaac aaatgcattt tatctttaga aataccctga ccattatctt
    10621 gtacaataat acgtacatgt gttgcagttt ctttaatcac tgacacgtca atatcgttac
    10681 cttgctttcg attcgtaaac gcatgtttga tggcattttc aactaaaatt tgaattaaaa
    10741 atggtggtac aagcacatcg cgatatttgt cttcaacatt aatattgata ttaaatcttc
    10801 ctggataacg tgcttgttcg agtgataagt atgcacgcac ttgacttaac tctttatcta
    10861 aagtaatcgt atgttgctta gagccttgta aattcgctct gaaaaaataa ctcaattcta
    10921 atagtaactc tcgtgccttt tcgctattta ttctaactaa agctgagatc gtgttaattg
    10981 aattgaagaa aaaatgtgga ctcacttgtg cctgtaatga cttaatctca gcatctttca
    11041 ataacttact ttgcgtttcg gcttcaccaa gttcaatttg gctactaaaa atatttgcca
    11101 atccttctgc aagttgacgt tccacaaaag ttaaatcatt agggtttgta aaatacatct
    11161 tcaatgtacc gacgatagaa ccatgcatct caagtggtat cacgatagct gctctaagcg
    11221 ggcaattcgg atgactacaa ccaatctctt ctttagtatg aacttctttc aactttcctg
    11281 atttcaatac atctttagac agacttgtta atatttcatt tgttggtatg tgatgatcac
    11341 tacctgcacc tacatgcgat aagatttcat ttttgcttgt aattgctacg gcagatactt
    11401 tcattaaatt tttaataatc atcgcaattt gctgtgccga ttctctattc aatccttctt
    11461 taaaatacgg caatgtctgg ttcatcaatt gcagtacatc atgtgtttga acagccttca
    11521 tttgctcctc ttgctttaat gttgaaatga taatagacat aaaaatcgcc gtaccaacgc
    11581 tattaacaat aatcattggt agtgcaatta atgatatgag gtcaaccgca tatgctttgt
    11641 cgtgggaaaa tgttaaaatg ctcaacattt gaatcatttc cataacaatt ccaatcatgg
    11701 cacttttcgc aatactcggg taacgcttgc gtctttgagc ttgtaagcca aaataaccag
    11761 caattatacc aataaatata gatgagataa gataaacttg tgcatccgcc ccacccatat
    11821 acactctgaa aatacctgaa ataacgccaa caaatagacc tacaaaaggg ccaccaacta
    11881 atcctgcgac acctatcgtt aatacacgtg tgttagctaa agatacatca tcatctaaac
    11941 ggaagtacac acttcctgac aaactatgtt gatgatcgat gacgatacca gttaaattag
    12001 acattaaggc aaacaaactg aaaataatac ataattgcca acgtgctttc catgtacgtc
    12061 gacgattcat taagttttta aaatatggaa tattcatcaa cacataggcc aaaataataa
    12121 ttaaacctac acgctcaagt aataacattg ttagcgatag cacgatagca cctcagtgaa
    12181 ttataattat gtttatttta tcataatagt gcatgtaaaa ttcatgaata aatt
//
LOCUS       DAKTGS010000084        12104 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.084, whole genome shotgun sequence.
ACCESSION   DAKTGS010000084 DAKTGS010000000
VERSION     DAKTGS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.084"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            874..3579
                     /gene="acnA"
                     /locus_tag="O0286_001844"
     CDS             874..3579
                     /gene="acnA"
                     /locus_tag="O0286_001844"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCY0170220.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3759..4226
                     /locus_tag="O0286_001845"
     CDS             3759..4226
                     /locus_tag="O0286_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0170221.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(4433..4729)
                     /locus_tag="O0286_001846"
     CDS             complement(4433..4729)
                     /locus_tag="O0286_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCY0170222.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(5105..5713)
                     /gene="plsY"
                     /locus_tag="O0286_001847"
     CDS             complement(5105..5713)
                     /gene="plsY"
                     /locus_tag="O0286_001847"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCY0170223.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5919..7910
                     /gene="parE"
                     /locus_tag="O0286_001848"
     CDS             5919..7910
                     /gene="parE"
                     /locus_tag="O0286_001848"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCY0170224.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7910..10312
                     /gene="parC"
                     /locus_tag="O0286_001849"
     CDS             7910..10312
                     /gene="parC"
                     /locus_tag="O0286_001849"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCY0170225.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDYSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10562..12022
                     /locus_tag="O0286_001850"
     CDS             10562..12022
                     /locus_tag="O0286_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0170226.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4235 a         1747 c         2320 g         3802 t
ORIGIN      
        1 tttaataaca agtagtatgt cattctagta gctagaacag atatactact tgtttgtttt
       61 tgtggaaaat tgagtatatt caaaaggata agaacgagaa tttcgttatt aaactacgaa
      121 ttctcgattt ttttatattt taagataggt ttatttctga aaacttaata gaaaggggtt
      181 tgacaaagct aaagtgaagt ttgacggtat aaacgcaaat taaatatact tttatagaaa
      241 attaactcag gctgggacat aaatcaatat tctatgctct acgaagttat attggcagta
      301 gttgactgaa cgaaaatgcg cttgtaacaa gcttttttca attctagtca ggggccccaa
      361 cacagagaat ttcgaaaaga aattctacag gcaatgcgag ttggggtgag ggccccaaca
      421 cagaagctga cgaaaagtca gcttacaata atgtgcaagt tggggatggg ccccaacaaa
      481 gagaaattgg attcccaatt tctacagaca atgcaagttg gggtgggacg acgaaataaa
      541 ttttgcgaaa atatcatttc tgtcccactc ccatcaaaag aatgacattg aaaaaaatat
      601 taaagtttgg tgccataatt ttctttctgc tcaatgaaat aatacgttta aaaattgaat
      661 tgaatcattc gcgaaattga taattattct caattaataa aaaatactta ttacacctta
      721 cctgtatgat aagttttgct tatatactct gattaaaaag tcaaaaccta aattaagcgt
      781 tttctaagca tgatgagttg tttatcaata aatataataa tacaatttaa tcaaggcata
      841 taaatataaa aatgtatcaa gggggatcat taaatggctg caaattttaa agagcaatca
      901 aaaaaacatt ttgacttgaa tggccaaagt tatacttact atgatttaaa agctgtagaa
      961 gagcaaggta ttactaaagt ttccaattta ccttattcaa ttcgtgtttt gttagaatct
     1021 ttacttcgtc aagaagatga ttttgtaatt acagacgatc atattaaagc tttaagtcag
     1081 tttggaaaag atggaaatga aggcgaggtg ccatttaaac cttctcgtgt tattttacaa
     1141 gatttcacag gtgtaccagc cgtagttgat ttagcttctt tacgtaaagc aatggatgac
     1201 gttgggggag atattactaa aattaatcca gaagtaccgg tggatttagt tattgaccac
     1261 tcagttcaag tggatagcta tgcaaatcca gaagctcttg aacgtaatat gaaattagaa
     1321 tttgaacgta actatgaacg ttatcagttt ttaaattggg caacgaaagc atttgataat
     1381 tacaatgcag ttcctcctgc aactggaata gttcaccaag ttaacttaga atatttagca
     1441 agtgttgtac atgttcgtga tgtagatggt gaaaaaactg catttccaga tacattagtt
     1501 ggtactgatt cacatacaac aatgataaat ggtattggcg tactaggatg gggtgttggt
     1561 ggtattgaag ctgaagctgg aatgcttgga caaccttctt atttcccaat tccagaggtt
     1621 attggtgtac gactagtaaa ttcattacca caaggcgcaa cagcaactga tttagcgtta
     1681 agagtaactc aagagctacg taaaaaaggt gttgttggta aatttgtgga gttctttggt
     1741 ccaggtgtac aacatttacc actagcagac cgtgctacaa ttgcaaacat ggcaccagag
     1801 tatggagcaa cttgcggatt cttcccagtt gatgatgaat ctcttaaata tatgaagtta
     1861 actggtagat cagacgaaca tatcgcgcta gtaaaagaat atttgaaaca aaaccatatg
     1921 ttctttgatg ttgagaaaga agatcctaat tatacagatg ttatcgaatt ggatttatca
     1981 acagttgaag catcgctttc aggaccaaaa cgtcctcaag atttaatttt cttaagtgat
     2041 atgaaatcat catttgaaaa ttctgtaaca gctccagcag gcaaccaagg acacggttta
     2101 gataaaagtg aatttgataa gaaagctgaa attaacttta aagatggatc aaaagctaca
     2161 atgaaaacag gtgatattgc aatagcagca attacatcat gtacaaatac atctaaccct
     2221 tatgtaatgt taggtgcagg tttagttgct aaaaaagcag ttgaaaaagg cttgaaagtt
     2281 cctgaatacg ttaaaacttc tctagcacca ggatcaaaag ttgttaccgg atatttaaga
     2341 gatgctggct tacaacctta tttagatgat ttaggcttca acttggttgg ttatggatgt
     2401 acaacttgta tcggtaattc aggtccttta ttaccagaaa ttgaaaaagc gattgctgat
     2461 gaggacctat tagtgacatc tgtattatct ggtaaccgta actttgaagg tcgtatccat
     2521 cctcttgtta aagccaatta cctagcttca ccacagttag ttgttgctta tgcattagct
     2581 ggaacggttg atattgattt acaaaatgaa cctattggta aaggtaatga cggtgaagat
     2641 gtatatttga aagatatttg gccatcaatt aaagaagttt cagataccgt tgatagtgtt
     2701 gtaacacctg aattatttat tgaagaatat aataacgtat acaataacaa cgaattatgg
     2761 aatgagattg atgtaactga tcaacctcta tatgactttg atcctaattc aacatacatt
     2821 caaaatccat cattcttcca aggattatct aaagaaccgg gtacgattgt tccattaaat
     2881 ggtttacgtg ttatgggtaa attcggtgat tctgtgacaa ctgaccacat ctctccagca
     2941 ggtgcaattg gtaaagatac gccagctggt aaatatttac aagatcatca agtgcctatt
     3001 cgtgaattta attcatatgg ttcaagacgt ggtaatcacg aagtaatggt tcgaggtacg
     3061 tttgctaata tacgtattaa aaaccaatta gcgccaggta ctgaaggtgg ttttacaact
     3121 tattggccaa caaatgaagt aatgcctatc tttgatgctg caatgaaata taaagaagat
     3181 ggtacaggtt tagttgtatt agctggtaac gattatggta tgggttcatc tcgtgactgg
     3241 gcagcaaaag gtacaaactt attaggtgtt aaaacagtta ttgcacaaag ttatgaacgt
     3301 atccatcgtt caaatttagt tatgatgggt gtattaccat tagagtttaa aaaaggtgaa
     3361 tcagctgatt ctcttggtct agatggtaca gaagaaattt ctgttaatat tgatgaaaat
     3421 gttcaaccac atgactacgt caaagttact gctaagaagc aagatggtga tttggtagaa
     3481 tttgacgcta tggttcgttt tgactcactt gttgaaatgg attactatcg tcacggtgga
     3541 attttacaaa tggttttaag aaataaatta gcgcaataaa aaatagatat cacagtaaaa
     3601 ttttaatcgg tatttgaggc actttgatag tgctcaaaaa tacttctata aactttatga
     3661 tataataggt cggttcaaaa cctttgtgtt cttggccggc ctcttcttgt attttatgtt
     3721 agaatgagat ataaagttaa actgaaagaa ggcttaaaat gatatatagt attacagaaa
     3781 tagaagcgcg ttatgctgaa actgataaga tgggtgtaat ttatcacggg aattatgcaa
     3841 cttggtttga agttgcgcgg ttggattata tatcgaagtt aggttttagt tatgctgata
     3901 tggaaaaaca aggaatcatt tcacctgtga ctgacctcaa tgtcaattat aaaaagtcta
     3961 ttttttatcc agaaaaagtt aaagttaaaa cttgggttga aaaatattcg agattacgtt
     4021 cagtgtataa atatgagatt tttaatgaaa aaggtgaact tgcaactaca ggttccacag
     4081 aattgatttg cattaaagaa gatactttta agcctatacg gttggatcgt tatttcccag
     4141 attggcatga agcttatagt aaagtgcaaa cgctcaataa tgaagggaaa acagtagaga
     4201 taatggatgg tattgattct ttataagtta ctgtaaatgt ttggagaggc aatattaaca
     4261 attaatctat aaaaaatagc caatacgtta acattaatgt aattagcgtg aagataagct
     4321 aatatatagc aaaaacgaac ttgttcatac aacatcagat gtgctaaacc aaagtacaac
     4381 ttccgaggtt caggacacat cagaatgtgt aaacaagttc gttttatttt agttattttg
     4441 tggaataaga aatttcatct tcgtgatcaa ctacatttac aataatgtgg tcatcttcaa
     4501 agtaccacaa atctttttcc gctacgacaa catttaaatc gtcatattgt tgttcatagc
     4561 caatatcaac atcttccttt ggttcaactg taaaagcagg actaaatcct tgcttgagtt
     4621 ggaattcgcc accatatctt acaaaaaaca cgagcacttt attattttca ggcaactcaa
     4681 gttcattttt aaaccaagtt actgctgcat cagtaagttc tatttgcata acaatgacct
     4741 cctatattta ttacagaatg ggagtgggcc aacttgtatt atttgtggaa tttcttatcg
     4801 aaattctctg tgttggagcc caccccaacc tgctcattat tgtaagctga atttccataa
     4861 gcttctatgt tgggaccccc gaccagaatt gaaaaaacct agttataagc gcattttgtt
     4921 cggttaacta cagctaatat aacattgtgg agcctatgac attgatttat gtctcggtct
     4981 caaatgttct tgtaaaaaaa ctaagtatag ttgaatttta agttaaaaaa attgtttttg
     5041 caaaaataaa ataatccatc aactgcaaaa aaatatgcaa tgatggacta atgtcaaaaa
     5101 attgttacat ccattttatt ttaggttctt cgcctctaaa aatccttgcg atattagagc
     5161 gatgtctaat tatcaatatg attgaaacta agaaactaac gactaataaa atatagtctt
     5221 gaatgataag cgagccaatc acacagcaaa ttgctgcaac gatacttgct aaagaaacat
     5281 atttaaaaat cttcaataca ataaagaaga taattgcaag tattagtaaa agtatcggat
     5341 tgactcccaa gacgacacct gcactagttg caacagcttt gccaccttgg aattttaaat
     5401 aaacaggata aacgtgtcca agtatagcga ataagccaac aattaaacca tttgtaaaaa
     5461 aagtactaat agggccatct gcgtgaactt gtaaccataa agggaagaaa acagttatga
     5521 accctttgaa aatatctaga aatgttacca agaatcctgc aggacgacct aatactctaa
     5581 agctattagt agcgccagta ttaccactac caaattgtct aatatctttt ttgaaaaata
     5641 attttccaat tacgaatcca cttgggaaag cgccgataag ataacttagt agtaacatga
     5701 cgattatcat cataaatatt acacatcctt taatatctta ggactatttt atcatattca
     5761 tttaaattac ggctaaaaat tttaaaaacg gggattaata tatggaatta agctatgaaa
     5821 gttaattgat acttgcattt tacgctgatt tatataagaa taactattgt atagttttaa
     5881 aaacgaacgt acgtttgcag gaggcgaaat cattggcaat gaataaacaa aataattatt
     5941 cagatgattc aatacaggtt ttagaggggt tagaagcagt tcgtaaaaga cctggtatgt
     6001 atattggatc aactgataaa cggggattac atcatctagt atatgaaatt gtcgataact
     6061 ccgtcgatga agtattgaat ggttacggta acgaaataga tgtaacaatt aataaagatg
     6121 gtagtatttc tatagaagat aatggacgtg gtatgccaac aggtatacat aaatcaggta
     6181 aaccgacagt cgaagttatc tttactgttt tacatgcagg aggtaaattt ggacaaggcg
     6241 gctataaaac ttcaggtggt cttcacggtg ttggtgcttc agttgtaaat gcattgagtg
     6301 aatggcttga agttgaaatc catcgagatg gtaatatata tcatcaaagt tttaaaaacg
     6361 gtggttcgcc atcttctggt ttagtgaaaa aaggtaaaac taagaaaaca ggtaccaaag
     6421 taacatttaa acctgatgac acaattttta aagcatctac atcatttaat tttgatgttt
     6481 taagtgaacg actacaagag tctgcgttct tattgaaaaa tttaaaaata acgcttaatg
     6541 atttacgcag tggtaaagag cgtcaagagc attaccatta tgaagaagga atcaaagagt
     6601 ttgttagtta tgtcaatgaa ggaaaagaag ttttgcatga cgtggctaca ttttcaggtg
     6661 aagcaaatgg tatagaggta gacgtagctt tccaatataa tgatcaatat tcagaaagta
     6721 ttttaagttt tgtaaataat gtacgtacta aagatggtgg tacacatgaa gttggtttta
     6781 aaacagcaat gacacgtgta tttaatgatt atgcacgtcg tattaatgaa cttaaaacaa
     6841 aagataaaaa cttagatggt aatgatattc gtgaaggttt aacagctgtt gtgtctgttc
     6901 gtattccaga agaattattg caatttgaag gacaaacgaa atctaaattg ggtacttctg
     6961 aagctagaag tgctgttgat tcagttgttg cagacaaatt gccattctat ttagaagaaa
     7021 aaggacaatt gtctaaatca cttgtgaaaa aagcgattaa agcacaacaa gcaagggaag
     7081 ctgcacgtaa agctcgtgaa gatgctcgtt caggtaagaa aaacaagcgt aaagacactt
     7141 tgctatctgg taaattaaca cctgcacaaa gtaaaaacac tgaaaaaaat gaattgtatt
     7201 tagtcgaagg tgattctgcg ggaggttcag caaaacttgg acgagaccgc aaattccaag
     7261 cgatattacc attacgtggt aaggtaatta atacagagaa agcacgtcta gaagatattt
     7321 ttaaaaatga agaaattaat acaattatcc acacaatcgg ggcaggcgtt ggtactgact
     7381 ttaaaattga agatagtaat tataatcgtg taattattat gactgatgct gatactgatg
     7441 gtgcgcatat tcaagtgcta ttgttaacat tcttcttcaa atatatgaaa ccgcttgttc
     7501 aagcaggtcg tgtatttatt gctttacctc cactttataa attggaaaaa ggtaaaggca
     7561 aaacaaagcg agttgaatac gcttggacag acgaagagct taataaattg caaaaagaac
     7621 ttggtaaagg cttcacgtta caacgttaca aaggtttggg tgaaatgaac cctgaacaat
     7681 tatgggaaac gacgatgaac ccagaaacac gaactttaat tcgtgtacaa gttgaagatg
     7741 aagtgcgttc atctaaacgt gtaacaacat taatgggtga caaagtacaa cctagacgtg
     7801 aatggattga aaagcatgtt gagtttggta tgcaagagga ccaaagtatt ttagataatt
     7861 ctgaagtaca agtgcttgaa aatgatcaat ttgatgagga ggaaatctag tgagtgaaat
     7921 aattcaagat ttatcacttg aagatgtttt aggtgatcgc tttggaagat atagtaaata
     7981 tattattcaa gagcgtgcat tgccagatgt tcgtgatggt ttaaaaccag tacaacgtcg
     8041 tattttatat gcaatgtatt caagtggtaa tacacacgat aaaaatttcc gtaaaagtgc
     8101 gaaaacagtc ggtgatgtta ttggtcaata tcatccacat ggagactact cagtgtacga
     8161 agcaatggtc cgtttaagtc aagactggaa gttacgacat gtcttaatag aaatgcatgg
     8221 taataatggt agtatcgata atgatccgcc agcggcaatg cgttacactg aagctaagtt
     8281 aagcttacta gctgaagagt tattacgtga tattaataaa gagacagttt ctttcattcc
     8341 aaactatgat gatacgacac tcgaaccaat ggtattgcca tcaagatttc ctaacttact
     8401 agtgaatggt tctacaggta tatctgcagg ttacgcgaca gatataccac cacataattt
     8461 agctgaagtg attcaagcaa cacttaaata tattgataat ccggatatta cagtcaatca
     8521 attaatgaaa tatattaaag gtcctgattt tccaactggt ggtattattc aaggtattga
     8581 tggtattaaa aaagcttatg aatcaggtaa aggtagaatt atagttcgtt ctaaagttga
     8641 agaagaaact ttacgcaatg gacgtaaaca gttaattatt actgaaattc catatgaagt
     8701 gaacaaaagt agcttagtaa aacgtatcga tgaattacgt gctgacaaaa aagtcgatgg
     8761 tatcgttgaa gtacgtgatg aaactgatag aactggttta cgaatagcaa ttgaattgaa
     8821 aaaagatgtg aacagtgaat caatcaaaaa ttatctttat aaaaactctg atttacagat
     8881 ttcatataat ttcaacatgg tcgctattag tgatggtcgt ccaaaattga tgggtattcg
     8941 tcaaattata gatagttatt tgaatcacca aattgaggtt gttgcaaata gaacgaagtt
     9001 tgaattagat aatgcagaaa aacgtatgca tatcgttgaa ggtttgatta aagcgttgtc
     9061 aattttagat aaagtaatcg aattgattcg tagctctaaa aacaagcgtg acgctaaaga
     9121 aaaccttatc gaagtatacg agttcacaga agaacaggct gaagcaattg taatgttaca
     9181 gttatatcgt ttaacaaata ctgacatagt tgcgcttgaa ggtgaacata aagaacttga
     9241 agcattaatc aaacaattac gtcatattct tgataaccat gatgcattat tgaatgtcat
     9301 aaaagaagaa ttgaatgaaa ttaaaaagaa attcaaatct gaacgactgt ctttaattga
     9361 agcagaaatt gaagaaatta aaattgacaa agaagttatg gtgcctagtg aagaagttat
     9421 tttaagtatg acacgtcatg gatatattaa acgtacttct attcgtagct ttaatgctag
     9481 cggtgttgaa gatattggtt taaaagatgg tgacagttta cttaaacatc aagaagtaaa
     9541 tacgcaagat accgtactag tatttacaaa taaaggtcgt tatctattta taccggttca
     9601 taaattagca gatattcgtt ggaaagaatt gggacaacat gtatcacaaa tagttcctat
     9661 cgaagaagat gaagtggtta ttaatgtctt taatgaaaag gactttaata cagatgcatt
     9721 ttatgttttt gcgactcaaa atggcatgat taagaaaagt acagtgcctc tatttaaaac
     9781 aacgcgtttt aataaacctt taattgctac taaagttaaa gaaaatgatg atttgattag
     9841 tgttatgcgc tttgaaaaag atcaattaat taccgtcatt actaataaag gtatgtcatt
     9901 aacgtataat acaagtgaac tatcagatac cggattaagg gcagctggtg ttaaatcaat
     9961 aaatcttaaa gctgaagatt tcgttgttat gacagaaggt gtttctgaaa atgatactat
    10021 attgatggcc acacaacgcg gctcgttaaa acgtattagt tttaaaatct tacaagttgc
    10081 taaaagagca caacgtggaa taactttatt aaaagaatta aagaaaaatc cacatcgtat
    10141 tgtagctgca catgtagtga caggtgaaca tagtcaatat acattatatt caaaatcaaa
    10201 tgaagaacat ggtttaatta atgatattca taaatctgaa caatatacaa atggctcatt
    10261 cattgtagat acagatgatt ttggtgaagt aatagacatg tatattagct aaaaactata
    10321 tgcaatcacg aaattaaatg ataaaataca gtaatgttaa attttgacta aattcaaggg
    10381 atttatatta aatgctgacc aagtacttat cgttaaatta gcgatatact ttgaccaaac
    10441 taatataaat ctcttgtatt gtataagaaa tgatgatgta tctctatatt tactgaaggt
    10501 tcaattcggt ctaattttat agaaggtaca tgattcatgt agaggatgcg aggggtttat
    10561 tttgaaagat ttcgatagtt taatacctgg atggtttaaa gaatttgtcc atgttggtac
    10621 cgatttaata tggtctcaat atttaattgg tctattattg acagctggat tcttctttac
    10681 gattagttct aaattcgttc aattacgaat gttacctgaa atgtttagag ctttagtaga
    10741 acgtccagaa actttagaag atggtaagaa gggtatttcg ccattccaag catttgcgat
    10801 tagtgctggt tcgagagttg gtactggtaa tattgctggt gttgcgactg cgattgtttt
    10861 aggcggtcca ggtgcagtat tttggatgtg ggttattgca tttataggtg cagcgagtgc
    10921 atttatagaa gcgactttgg ctcaggttta taaagtacat gataaagatg gtggattccg
    10981 tggtggtcca gcttactata ttactaaagg tttaaatcaa aaatggctag gtatcgtatt
    11041 tgcgatttta attacaatta catttgcatt tgtatttaac acagtgcaat ctaatacaat
    11101 tgcggagtcg ttaaatacgc aatataatat tagtccagta atcacaggta ttattttagc
    11161 aatcgtaaca gctattatta tatttggtgg tgtacgtagt attgctacgt tatcttcgtt
    11221 aattgtaccg attatggcta tcatttacat tggtatggtt ttagtaatat tgctatttaa
    11281 tttagatcaa attgttccta tgataggtac gattattaaa agtgcatttg gtatcgaaca
    11341 agtaactggt ggcgctgtag gtgctgcggt tcttcaaggt atcaaacgtg gtttattctc
    11401 taacgaagct ggtatgggtt ctgcgccgaa tgcagcggca actgctgccg taccacaccc
    11461 tgttaagcaa ggtttaatcc aatcattagg tgtgttcttt gatacaatgt tggtttgtac
    11521 agcaactgca atcatgattt tactatattc aggactgaaa tttggtgata acgcacctca
    11581 aggtgttgca gttactcaat cagcacttaa tgagcattta ggttctgctg gaggtatttt
    11641 cttaacaata gcagttacac tgtttgcatt ttcatctgtt gtaggtaatt actattacgg
    11701 tcaatctaat attgaatttt tatcaacaaa ccgtgtaata ttatttatct ttagatgtct
    11761 tgttgtagta cttgtctttg tcggtgcagt tgtaaaaaca gaaacagtat ggaatacggc
    11821 agacttattt atgggcttaa tggcaattgt aaacattatt tccattatag gactgtccaa
    11881 tgtagctttt gcattgatga aagattatca aaagcagaaa aaagaaggca agaaccctgt
    11941 ctttaaacct gaaaacttag aaattaactt atttggaatt agtgcttggg gcgctaacaa
    12001 atataagaac tctgataaat aaatattttt aatgtaacga ttcattatat aaaaatcaaa
    12061 taaaccctga ggttttcaaa ttaaagcgaa aatctcaggg tttt
//
LOCUS       DAKTGS010000085        12095 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.085, whole genome shotgun sequence.
ACCESSION   DAKTGS010000085 DAKTGS010000000
VERSION     DAKTGS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.085"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..275
                     /locus_tag="O0286_001851"
     CDS             <1..275
                     /locus_tag="O0286_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease
                     C-terminal domain-containing protein"
                     /protein_id="HCY0170227.1"
                     /translation="QETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIR
                     INQQPDKRSIDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS"
     gene            297..815
                     /locus_tag="O0286_001852"
     CDS             297..815
                     /locus_tag="O0286_001852"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCY0170228.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            1243..3432
                     /locus_tag="O0286_001853"
     CDS             1243..3432
                     /locus_tag="O0286_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCY0170229.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            3444..3896
                     /locus_tag="O0286_001854"
     CDS             3444..3896
                     /locus_tag="O0286_001854"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCY0170230.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            3893..4768
                     /locus_tag="O0286_001855"
     CDS             3893..4768
                     /locus_tag="O0286_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0170231.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            5229..6491
                     /locus_tag="O0286_001856"
     CDS             5229..6491
                     /locus_tag="O0286_001856"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCY0170232.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            6507..8273
                     /gene="aspS"
                     /locus_tag="O0286_001857"
     CDS             6507..8273
                     /gene="aspS"
                     /locus_tag="O0286_001857"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCY0170233.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            8351..8541
                     /gene="ssrS"
                     /locus_tag="O0286_001858"
     ncRNA           8351..8541
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O0286_001858"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            8606..8734
                     /locus_tag="O0286_001859"
     CDS             8606..8734
                     /locus_tag="O0286_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170234.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            8734..9507
                     /locus_tag="O0286_001860"
     CDS             8734..9507
                     /locus_tag="O0286_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCY0170235.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(9668..10942)
                     /locus_tag="O0286_001861"
     CDS             complement(9668..10942)
                     /locus_tag="O0286_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCY0170236.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            11027..11449
                     /locus_tag="O0286_001862"
     CDS             11027..11449
                     /locus_tag="O0286_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0170237.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            11549..11731
                     /locus_tag="O0286_001863"
     CDS             11549..11731
                     /locus_tag="O0286_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0170238.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            11771..11917
                     /locus_tag="O0286_001864"
     CDS             11771..11917
                     /locus_tag="O0286_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170239.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
BASE COUNT         4372 a         1661 c         2361 g         3701 t
ORIGIN      
        1 aacaagaaac aaatatccag aaagagggta tgttattgtg tcaacattta agtgtgaaac
       61 cagatacact taaattcatg ttgaaagttt tcttagactt aaaatttgta acacaagaag
      121 atggtttaat tcgaatcaat caacaacctg ataaaagatc gattgattcc agcaaagtat
      181 atcaattaag acaacaacgt atggatgttg aaaagcaatt attatatcaa gatttttcag
      241 aaataaaaaa ttggataaag tcacaattgt cgtgagcaat ttaggaggaa atattaatgg
      301 atttaaagca atacgtatca gaagttcaag attggccgaa accaggtgtt agtttcaagg
      361 atattactac aattatggat aatggtgaag catatggcta tgcaacagat aaaattgtag
      421 aatacgcaaa agacagagat gttgatatcg ttgtaggacc tgaagcgcgt ggctttatca
      481 ttggctgtcc tgtagcttat tcaatgggga ttggctttgc acctgttaga aaagaaggga
      541 aattacctcg tgaagtcatt cgttatgagt atgacctaga atatggtaca aatgttttaa
      601 caatgcacaa agatgcaatt aaaccaggtc aacgtgtgtt aattacagat gatttattag
      661 ctactggtgg tacgattgaa gcagcaataa aattagttga aaaattaggc ggtatcgtag
      721 taggtattgc atttataatt gaattgaaat atttaaatgg tattgaaaaa attaaagatt
      781 acgatgttat gagtttaatc tcatacgacg aataataaat aatataattt tatcaaatga
      841 aatccttcat caaatgtata agaaccaatg acttaattaa aaaagttgtt taagttttct
      901 taacatgaga tgttaggatt ttttatttac tgaaaatgtt agatgattga gcattatacc
      961 ttaataacat cgtttattta tttcataaat tgtagtatca tagaactaat atttaaaaaa
     1021 tgaaacagta gatttaggtc gaatttttgt aaaagtttta aaagtaggaa tagtatacaa
     1081 attaaactcg ctcaagtaaa attaatatta cgattaatga cgacaggata aatatttatc
     1141 gtcgacggac gtatgattgg tgtgggacaa atactattca acaagagtac ctaaatcatt
     1201 gtttaaggcg aagtaataaa tatgaatggg gtgtatcata taatgaacaa cgaatatcca
     1261 tatagtgcag acgaagttct tcacaaagca aaatcatatt tgtcagcaga tgaatatgag
     1321 tatgttttaa aaagctatca tattgcttat gaagcacata aaggtcagtt ccgaaaaaac
     1381 ggattaccat acattatgca tcctatacaa gttgcaggta ttttaacaga aatgcgatta
     1441 gacggaccga cgattgtcgc aggttttttg catgatgtaa ttgaagatac accgtataca
     1501 tttgaagatg taaaagaaat gttcaatgaa gaagttgctc gaattgttga tggtgtgacg
     1561 aagcttaaaa aagtaaaata ccgctcaaaa gaagaacaac aagctgaaaa tcatcgcaag
     1621 ttatttattg cgattgccaa agatgtacgc gtaattttgg tgaaattagc agacagatta
     1681 cataatatgc gtaccttgaa agccatgccg cgcgaaaaac aaattagaat ttctcgagaa
     1741 acattagaaa tttatgcacc attagcacat cgtcttggta ttaatacaat caaatgggaa
     1801 ctagaagata cggctcttcg ttatattgat aatgtgcaat attttagaat agtcaattta
     1861 atgaagaaga aacgtagtga acgtgaagcg tatatcgaaa cggctattga tagaatacgt
     1921 actgaaatgg accgaatgaa tatcgaaggc gatataaatg gtagacctaa acatatttac
     1981 agtatttatc ggaaaatgat gaagcagaaa aaacaatttg atcaaatttt tgatttgttg
     2041 gcgatacgtg ttattgtcaa ttctattaat gattgttatg cgatacttgg gttggtgcat
     2101 acgttatgga aaccgatgcc aggacgtttt aaagattata ttgcaatgcc taaacaaaat
     2161 ttgtatcagt cattgcatac tacagtagta ggcccaaatg gagacccgct cgaaatccaa
     2221 atacgaacgt ttgatatgca cgaaattgct gagcatggtg ttgcagcaca ctgggcttac
     2281 aaagaaggta aaaaagtaag tgaaaaagat caaacttatc aaaataagtt aaattggtta
     2341 aaagaattag ctgaagcgga tcatacatcg tctgacgctc aagaatttat ggaaacctta
     2401 aaatatgact tacagagtga caaagtatac gcatttaccc cagcgagtga tgttattgag
     2461 ttgccatatg gtgctgtgcc gattgatttt gcttatgcga ttcacagtga agtaggtaat
     2521 aagatgattg gtgccaaggt gaatggcaaa attgtaccaa ttgactatat tttacaaaca
     2581 ggcgatattg ttgaaatacg tactagtaaa cattcatatg gaccaagtcg tgattggttg
     2641 aaaattgtta aatcgtctag tgccaaaggt aaaattaaaa gtttcttcaa aaaacaagat
     2701 cgttcatcta atattgaaaa aggccgaatg atggttgaag ctgaaataaa agagcaagga
     2761 tttagagtcg aagatatttt gacagagaaa aatattcagg ttgttaatga aaaatataac
     2821 tttgcaaatg aagatgattt attcgcagct gtaggatttg gcggcgtgac atccttacag
     2881 attgttaata aattaactga aagacaacgt attttagata aacaacgtgc tttaaatgaa
     2941 gcacaagaag ttacgaaatc attgcctatt aaagacaaca tcattactga tagtggtgtc
     3001 tatgtagaag gtttagaaaa tgtacttatc aagttgtcaa aatgttgtaa tcctatacca
     3061 ggtgatgata ttgtaggtta tatcaccaaa ggtcacggta ttaaagtaca tcgcactgat
     3121 tgcccaaata ttaagaacga aactgaacga ctaattaatg ttgaatgggt aaaatcaaaa
     3181 gacgcaactc aaaaatatca ggttgattta gaggtaactg cgtatgaccg aaatggcttg
     3241 ttgaatgaag tactacaagc tgttagctcg acagccggca atttaattaa agtttcagga
     3301 cgttcagata ttgataaaaa tgcaataata aatattagtg tcatggtgaa aaacgtgaat
     3361 gatgtttatc gtgtggtaga aaagatcaaa caacttggtg atgtttatac agtaacaaga
     3421 gtttggaact agaggtgcaa aatatgaaag tagttgtaca aagagttaaa gaagcatcgg
     3481 tgacgaatga tacattaaat aatcaaatca aaaaaggata ttgtttatta gtcggtatcg
     3541 gtcagaactc tacagagcaa gatgcagatg taattgcaaa gaaaattgct aatgcaagat
     3601 tatttgaaga tgacaataat aaattaaact ttaatatcca acaaatgaat ggtgaaatac
     3661 tatcagtttc acaatttact ctctatgcag atgtaaaaaa aggtaaccgt ccaggtttct
     3721 caaattctaa aaatcctgat caagcggtaa aaatttatga gtattttaat gatgcgctac
     3781 gagcgtatgg tcttactgtg aaaacaggtg aatttggaac acacatgaat gttagcataa
     3841 ataatgatgg tccagtcact attatttatg aaagtcagga cggcaaaatt caatgaaaaa
     3901 aatagaggca tggttatcta aaaagggtct taaaaataaa cgtactctaa tagtagtgat
     3961 tgcctttgtc ttatttatca tctttttatt tttattgctg aatagcaata gtgaagatag
     4021 tgggaacatc acgataactg aaaatgctga attacgtaca ggtccaaacg ctgcgtatcc
     4081 agtcatatat aaagttgaaa aaggtgacca ttttaaaaag attggtaaag taggtaaatg
     4141 gattgaagtt gaagatacat ccagtaatga aaaaggttgg atagctggat ggcacacaaa
     4201 tttagatatt gtcgcggata atacgaagga gaaaaatcct ttgcaaggta aaacaatagt
     4261 gcttgatcct ggtcatggag gtagtgacca gggtgcttca agcaatacta aatataaaag
     4321 tttagaaaaa gattatacgt tgaaaacagc aaaagaattg cagcgtactt tagaaaaaga
     4381 aggcgcaact gttaagatga caagaacaga cgatacatat gtttcactag aaaatcgtga
     4441 tatcaaaggc gatgcctatt tgagtataca taatgatgcg ttagaatcat ctaatgcaaa
     4501 tggaatgaca gtttattggt atcatgataa tcaaagagct ttagcagata cgttagacgc
     4561 tacgattcag aagaaaggtc tactttctaa tcgcggttca agacaagaaa attatcaagt
     4621 gttaagacaa acaaaagttc ctgctgtttt attagaatta ggttatatta gtaacccaac
     4681 tgatgaaacg atgattaaag atcaattaca tagacaaatt ttagaacaag caattgttga
     4741 tggccttaaa atttattttt ctgcgtaggg cttgcaaaaa tatgtgaaag tagttatcat
     4801 tgatattgaa ttttataact aaaaccgtta gtattcttga aatggtaaat gaaataggta
     4861 gcaatctaac taagattgtg taggaatata atccatagac tgaaagatta tgctgagtag
     4921 tttatataca ttgaacacaa gaagaggtgc tttatgaaaa gtaaagccgt taaacgtacg
     4981 ttaaacgttt tgagtgggtt tattaaatgc acgcttataa aaagtaatga tgattacaat
     5041 taggcatgtt ttttaaacca atgagggtgg caacacggat attcgtccct tgtgcagttt
     5101 caggctgtgc aagggctttt tcttttgctt ttaagattat gatttatcgt gcaaagttaa
     5161 gtggtcgtat atagttttag ttttaaaaag gtaattaaat aaaatagttt gccgagggag
     5221 atgtcaaaat gattaaaata cctagaggga cgcaggatat tttacctgaa gattcaaaga
     5281 aatggcgtta cattgaaaat caattagatg aattaatgac attttataat tataaagaaa
     5341 taagaacacc aatttttgaa agtacagatc tttttgcaag aggtgttggt gattcaaccg
     5401 atgtcgtaca aaaagaaatg tatacattta aagataaagg cgatagaagt attacattaa
     5461 gacctgaggg aacagctgca gttgtgcgtt catatattga acataaaatg caaggtaatc
     5521 caaaccaacc aattaaactt tattacaatg gaccgatgtt tagatatgaa cgtaagcaaa
     5581 aaggacgcta tcgtcaattt aatcaatttg gtgtagaagc tattggtgct gaaaatccta
     5641 gcgtagatgc agaagtatta gctatggtta tgcatattta tcaatcattt ggattaaaac
     5701 atttaaagct tgttattaat agtgtagggg atatggcgtc tcgaaaagaa tataacgaag
     5761 cgttagtgaa acactttgaa ccagtaattc atgaattttg ttcagattgt caatcacgtt
     5821 tgcatacaaa tccgatgcga attttggatt gtaaagtaga ccgtgataaa gaagcgatta
     5881 agactgcacc tagaatcact gatttcttaa atgaggaatc taaggcatat tatgaacaag
     5941 taaaagctta tttagatgat ttaggtattc catatattga agatcctaac ttagttcgtg
     6001 gattggatta ttatacacat acagcatttg aattaatgat ggataaccct aactatgatg
     6061 gtgccattac aacgctttgt ggtggtggcc gttataatgg tttattagaa ttgctagatg
     6121 gtccaagtga aacaggtatt ggttttgcgc taagtataga acgattattg cttgcacttg
     6181 aagaagaagg tatcgaatta gatattgaag aaaacttaga tttattcatt gttacaatgg
     6241 gtgatcaagc agatcgatat gctgtgaagc tattaaatca tttgagacat aatggtatta
     6301 aagcagataa agactattta cagcgtaaaa ttaaaggaca aatgaaacaa gcagaccgtt
     6361 taggtgccaa gtttacaatc gttattggtg atcaagaatt agaaaataat aaaatcgatg
     6421 ttaaaaatat gacaactggt gaatctgaaa caattgaatt agacgcatta gtcgaatatt
     6481 ttaagaagta gagagggcgt taaaatatga gtaagagaac aacttattgt ggattagtta
     6541 ctgaggcatt tttaggacaa gaaattacat taaaaggatg ggttaacaat cgtcgtgacc
     6601 ttggtggatt gattttcgtt gatttaagag atagagaagg aattgtacaa gtcgtgttta
     6661 atcctgcatt ttcagaagag gcattgaaaa ttgctgaaac agtacgttct gaatatgttg
     6721 tagaagttca aggtacagtt acgaagcgtg accctgaaac agttaatcct aaaattaaaa
     6781 ctggccaagt tgaagtacaa gttacaaata ttaaagtgat taataaatct gagacaccac
     6841 cattttctat aaatgaagaa aatgttaacg ttgatgaaaa tattcgatta aaataccgtt
     6901 atttagattt acgtcgtcaa gagttagcgc aaacatttaa aatgagacat caaattacac
     6961 gttctattcg tcaatatttg gatgatgaag ggttctttga catcgaaaca ccagtactaa
     7021 cgaagtcaac acctgagggt gcacgtgact atttagtacc atctcgtgtt catgatggtg
     7081 aattttatgc attaccacaa tcaccacaat tatttaagca attattgatg attagtggat
     7141 ttgacaaata ctaccaaatc gtaaaatgct tccgtgacga agatttacgt gcagatcgtc
     7201 aacctgaatt tacacaagtc gatattgaaa tgagttttgt agaccaagaa gatgtgatgc
     7261 aaatgggtga agaaatgctt aaaaaagttg ttaaagaagt taaaggcgtt gaaattaatg
     7321 gcgctttccc acgcatgaca tataaagaag cgatgcgtcg ctatggttct gataaaccag
     7381 atacacgttt tgaaatggaa ttaattgacg tttctcaatt aggacgtgat atggacttta
     7441 aagtatttaa agatactgtt gaaaatgatg gtgaaattaa agcaattgtc gctaaaggtg
     7501 cagctgaaca atatactcgt aaagatatgg atgctttaac agaatttgta aacatctatg
     7561 gtgctaaagg attagcgtgg gttaaagttg tggaagatgg tttgacaggt ccaattggac
     7621 gtttctttga aacagaaaat gttgaaacat tacttacatt aactggtgct gaagctggtg
     7681 acttagtaat gtttgttgca gacaaaccaa atgtcgttgc acaaagttta ggtgcattac
     7741 gtgtcaaatt agctaaagaa ttaggtttaa tcgatgaaac aaaattaaac ttcttatggg
     7801 tgacagattg gccattatta gaatatgatg aagatgcgaa acgttacgtt gcagcacatc
     7861 atccatttac atctccaaaa gaagctgata ttgctaagct tggcactgcg ccagaagaag
     7921 ctgaggcaaa tgcttatgac atagtattaa atggttatga attaggtggc ggttcaatca
     7981 gaattcatga tggtgagtta caagaaaaaa tgttcgaagt tcttggattt actaaagaac
     8041 aagcacaaga acagttcggc ttcttactag atgcatttaa atatggtgca ccaccacatg
     8101 gcggtattgc attaggatta gaccgattag tcatgttatt gactaataga acaaacttga
     8161 gagatacaat cgcattccct aaaacagcat ctgctacatg tttattaacg aatgcgcctg
     8221 gtgaagtttc tgataaacaa ttagaagaac tttctttgcg aattcgtcac taaattttaa
     8281 aaaatcaata aactttggtt tgttataatt tcattaattt taattatgtg gtaatataac
     8341 atcataagat agtcatctga gttgttcgta agtttgcttt ttaatttggg cctaacactc
     8401 tttgatcaag ggagcccaat gggttttctt gcagcgtaca cgccttatat gaaggacgta
     8461 caaaacaaga aacagggcac ccacctgtat ataacaggcc gaatgatcaa gctattataa
     8521 ctacggcaat acggactcta tcgatacgca agactttgtc ttgcgtattt ttatgcgttt
     8581 ctttaagttt ttaataaaga attagttggg ttttatagcg tttaataaat gtttcctcgt
     8641 tgaattttgg gaaaataatc aaagtgataa tgctaaatat aaaattttaa gttactatgt
     8701 acttatgtat aaatcagtga aagaggtaaa ataatgaaac atcaattttc aagaaatgaa
     8761 ctagcgattg gacaacaagg tttagattta ttgaagcaac aaactgttgt catacttggc
     8821 gttggaggtg ttggttcatt tgctgctgaa gcattagcaa gaacgaatat tggtcatatt
     8881 atattaatag ataaagatga tgttgatatt acaaatgtaa acagacaatt acacgcttta
     8941 actacaacgg taggacaaag taaagtttct ttgatggaag aacgtattaa attaattaat
     9001 ccagattgta aagtcacgcc cttacatatg ttttatacag aagagacata tgaagaaatt
     9061 ttcgataact acgatataga ttattttgta gatgcaagtg atacaataat gtacaaagta
     9121 cacttaatga aagagtgtct tgaccgaggg attaaagtga tttccagtat gggtgctgca
     9181 aataaaacag atccaacacg ttttacaatt tcagatatct ctaaaacaca taccgatcca
     9241 atggccaaaa ttattcgtca aaaattgaaa aaactaggaa ttcgtaaagg gattccagtt
     9301 gtattttcag atgaaagtcc aattgtcata agagaagatg taaaagatat agttggagat
     9361 aaaaatgcta tcaatcgaaa aggacaaatg ccaccttctt caaatgcctt tgtgccaagt
     9421 gttgttggat taatttgtgc aagttatgtg gtgaatgacg tattaaaaga tattccagtt
     9481 cgtcgcatta aagacaaagg gcaataattc attttgaaag ggataatttt caacgtaagg
     9541 caagtgtaac cacaccataa aactaaaact gactagttcg caagcatagt agaatatgcc
     9601 tcgtgtacta gtcagttttg atttgattat agctaaaata tcaaaggtaa tcgatttaat
     9661 cttattttta cggcctttgt ttaagtaagt tgttatatat ttctttgagt tgttgttcac
     9721 ttttagatgt cgtttttggt tcgtaataaa ttttgttttt tagtttatca ggtaaatatt
     9781 gctgtgaaac atagccatta acatattggt gtggatattt gtaaccaata gatcggccta
     9841 gatctttagc accttgataa tgtccatctt ttaaatggtt tggaatttgg cccacatgac
     9901 cgtttctaat atcggacaat gcactatcaa tggcactcat tgctgaattt gacttaggtg
     9961 ataagcatag ttcgattact gcttggctta gtggaattct agcttctggt aaacctagac
    10021 gttctgctga ttcaatagca gcaagtgttc tctgaccagc attaggtgag gctaagccta
    10081 tatcctcata gctaattaca agtaatcgtc gaactattgt aggtaaatct ccagcttcaa
    10141 ttaatcgtgc taaataatgt aaagcggcat tgacgtcgct accacggata gatttttgga
    10201 aagcgctcat aacatcgtaa tgcatgtcac catccttgtc acttacaaat gcaccttttt
    10261 gtaaacagtc tttagcatct tgcaatgtaa catgtcgata accgtctttg tcattatctg
    10321 cgcttaatac agctaattcc aatgcattta acgcactacg aacatcacct tggctttgtg
    10381 tagaaaagta ggtcatggca tcttcatcaa ttttgggttg atatgttttc aaaccattct
    10441 catcatcttc tattgcacga gttaacgctt ggcgcacatc ttcgtcattt aaaggatata
    10501 actcgaaaat ttgcgctctt gaacgaatcg ctggattgat agcatgataa ggatttgaag
    10561 ttgtagcacc gatcaagacg attttgccat tttctaaatg aggtaataaa aagtcttgtt
    10621 tagctttatc tagtcgatgt atttcatcta ataacaagat aacttgacca gacattttag
    10681 cttcttcaac aacaagttgc atatcttttt tagtgttagt tacagcattc aattgtctga
    10741 atttatattg cgtactgccc gaaattgctt tggcaatact tgttttgcct ataccaggtg
    10801 gaccataaaa aatcattgaa gttaattttt ttgtatcaac cattcttctg ataatgcctc
    10861 ttggtccaac taaatgttgt tgggaaatga tttcatctat attttttggg cgcattctcg
    10921 atgctaatgg ttctgtactc acatttgtca cacctttctt atactatctt aactcaaaaa
    10981 atgctaaaat gggaatatca atattcgaat taagtgaggt tatacaatga aaatttctac
    11041 taaagggaga tatggactta cattgatgat ttctcttgct aaaaaagagg ggcaaggatg
    11101 tatatcatta aagtcaattg ctgaagaaaa taatttgagt gatttatatt tagaacagct
    11161 tgtaggtcct ttaagaaatg cggggttaat tcgaagtgta cgcggtgcta aaggtggata
    11221 ccaattaaga gtgccagcgg aagaaatctc agcaggggat attataagac tgttagaagg
    11281 tccaattaca tttgttgaaa gtattgaatc agaaccacct gcgcaaaaac aactatggat
    11341 tcgcatgaga gatgcagtga gagatgtttt agataataca acattgaaat atttagcgga
    11401 atatgtagat acaagtgaag atttagacgg atacatgttt tatatttaat cgtataaata
    11461 ctatactata acataaaaac ttcatattat aatgtttagc gaacctcctt agtggtatat
    11521 aaatatatac atccaaggag gtttcactat ggcagacgaa agtaagttcg atcaatttaa
    11581 aggaaatgtt aaagaaacag taggtaacgt tactgataac aaagaattag aaaaagaagg
    11641 tcagcaagat aaagtgattg gtaaagcaaa agaagttgtt gaaaatgcta aaaacaaaat
    11701 aactgatgca attgataaac ttaaaaaata agttcaatta aactaatcaa atataagagg
    11761 agcgattaat atgagtttta tggataaagc taaagacgca gtagaaaaat ttaaaaatag
    11821 cgataatgaa caagttaaaa atgtaaaaga taagataaat gaatatacag gatcgaataa
    11881 cgaagagaaa aaagaaaatg aagataaaga gaaataaatt tctttaaatt ttggatatgc
    11941 ttttcaatat tttgaaagcg ccccatcaaa taaacactta cacagtgtaa agttgaaggg
    12001 gcgctttcat atttgcatta gaattttaag tgaatttgaa tcattactaa aataaatgat
    12061 tgaacaataa agacatcttt cattcaaaat gctgt
//
LOCUS       DAKTGS010000086        11948 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.086, whole genome shotgun sequence.
ACCESSION   DAKTGS010000086 DAKTGS010000000
VERSION     DAKTGS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.086"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(787..1230)
                     /locus_tag="O0286_001865"
     CDS             complement(787..1230)
                     /locus_tag="O0286_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170240.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(1395..1772)
                     /locus_tag="O0286_001866"
     CDS             complement(1395..1772)
                     /locus_tag="O0286_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170241.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            complement(2090..2680)
                     /locus_tag="O0286_001867"
     CDS             complement(2090..2680)
                     /locus_tag="O0286_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCY0170242.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(2677..2889)
                     /locus_tag="O0286_001868"
     CDS             complement(2677..2889)
                     /locus_tag="O0286_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCY0170243.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            2950..3496
                     /locus_tag="O0286_001869"
                     /pseudo
     CDS             2950..3496
                     /locus_tag="O0286_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3591..5207)
                     /gene="groL"
                     /locus_tag="O0286_001870"
     CDS             complement(3591..5207)
                     /gene="groL"
                     /locus_tag="O0286_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCY0170244.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(5283..5567)
                     /gene="groES"
                     /locus_tag="O0286_001871"
     CDS             complement(5283..5567)
                     /gene="groES"
                     /locus_tag="O0286_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCY0170245.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            5742..6485
                     /locus_tag="O0286_001872"
     CDS             5742..6485
                     /locus_tag="O0286_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0170246.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(6510..7763)
                     /locus_tag="O0286_001873"
     CDS             complement(6510..7763)
                     /locus_tag="O0286_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170247.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNAS
                     DGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEV
                     TDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGE
                     IGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMM
                     VVFSIMLFLFVKRNKKKNKNESQRR"
     gene            7960..8586
                     /locus_tag="O0286_001874"
     CDS             7960..8586
                     /locus_tag="O0286_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCY0170248.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            8947..9732
                     /locus_tag="O0286_001875"
     CDS             8947..9732
                     /locus_tag="O0286_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCY0170249.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(10329..10463)
                     /locus_tag="O0286_001876"
     CDS             complement(10329..10463)
                     /locus_tag="O0286_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCY0170250.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            10699..11268
                     /gene="agrB"
                     /locus_tag="O0286_001877"
     CDS             10699..11268
                     /gene="agrB"
                     /locus_tag="O0286_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCY0170251.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            11265..11405
                     /locus_tag="O0286_001878"
     CDS             11265..11405
                     /locus_tag="O0286_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCY0170252.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
BASE COUNT         3879 a         2037 c         1671 g         4361 t
ORIGIN      
        1 aaacaggtag ttttttattt attttaggcc gattaaatgt cttatgtctt taattaagta
       61 aaatgcacaa gtacaccttt aaaaagattt actcaaaact aaactatacc tagttaaccg
      121 cttgtgatta agaaatttta aatgtggttt cttttaatta tgtcaaccac ccataaggat
      181 tttaatcaag cactcattca ttcctgcact cgcaatatgc attacttata gaagtggatg
      241 cactctcata ttatggcaat accataactc ccgcaccata atgatttaaa taccattatg
      301 aatctcaatt acaaattagc acataaaaaa gacgcctccc ataagtctag tcaccaaatg
      361 gaaaacgtct cgtaattaaa atatatttgc gtgcattcta atttgtactt agaatacatc
      421 atactcgttc ataatgtaat tttctaatta gtcaaaacta tatacagttt tacatcattc
      481 ccggcattat gcactaataa aaacttactt taatacacgc tttaaagatt attaaatcag
      541 cgttttaatt tttagacaac gctattattt ttagtttttc aattctatta tgtcataatt
      601 atgtcactca aaaactgttt tccaacattg atttattgtt ggaaaatctc gaaaatctag
      661 tctatttctc caacaatcga agaattatgc ccttttttct ccctttaaat aagtcataat
      721 acgaggcata catgcaacat ttacaataaa atatgttcct acatcgtatt atacgaatgc
      781 tcacacttat agttgtttag taaacccaga tgtattgcta acatacccat tatcactata
      841 tagtttatgt gcagatagtc tttcatttct attaccacta tttagtgtta ttgctttaca
      901 attcaaccgt ttagaaaatt cttcagaatc agctaataat ctctttccat aacctttttt
      961 cctaaattca gaatgtataa caaacgcaag gattctcata tactctgcat ttttttcgta
     1021 aaacatcatt ttacacatac cacttaaacc aattatttta ttttctttta tcaaaagcag
     1081 taggaagtaa tcatcatgat tcgttatttt ctttagtctc ttttttaaat cattctcatt
     1141 tgttggataa cctagatcat catataattt acatagttga tttaattttt caaaatcact
     1201 gttattaaac aatcttgtaa ttatgctcat ataacccacc ctaaaaatat tatgtaccta
     1261 ttgttacata caatagatac gcaaaatcat ttctcactgc aaatcaggac gtttctcagc
     1321 gtaaacaaat catttctatt cttttgaaaa atatctgcat atcttcacag taattttata
     1381 acagattaat aaaattaaaa aaacataaca acaagtatta taataaatat tataatagct
     1441 atcgtattaa atagtttaga atatgcaaac tgaaaaataa aaatcacaat taaaatacta
     1501 agtaatgtaa atggtaatag catcatcaga tctttaacat tatttctttg ttcttgctcc
     1561 caatcgctaa tagccctaaa actacttttt ttatctatag gcgatgtaaa aataccatat
     1621 attgaaggtg ctatacctcc taaaatagca gttcccaaag ttgtcattac tgaaattact
     1681 gcgaaagtat catccgaaag caataaattc aaactaatgc attgtttatt acccatcgaa
     1741 tttattgacc aaatagctag agaaataaac aacccaaaat ttaaaataaa tgatatagta
     1801 atagcaattg tttacaaaac acgaaatttt tcatttttat ttatattatc catttttctc
     1861 cctttttctt aaatcatttt attatatatt acaataatca atctgaaatg ttgatgtaat
     1921 ttgaaaaaaa tatcatactt ttactcctga aaacctccct aaatcatcaa tatgaaaatc
     1981 agtattagga tattgcgttt acaactcttt taaatctcac tcattcttct catcgtctta
     2041 accgatacta tcaataaata ctggcaacac cataaaatat atacccaatt tataaaaacc
     2101 taattgactc ataatcttct aacggtatat catccacaat cacagtatga ttaggattat
     2161 cgttagatac atctttcact gccttatcta acccctcatc acctccgtcc cattcaccaa
     2221 tattaatgaa tataggtaca ttaccgttta tatcatgctt atctgtaaat aatttatgat
     2281 atttacccaa catatcacga gcttttaaac gatcactagg ttttattggt acctctatca
     2341 gttcaacatg ttcattatag actaattgta ctttgccact ttgtggattt tctttatatt
     2401 ctccacgctt gactacaact tctttcgttt ctgtttcatc accgactgcc gcattcgtta
     2461 gcacatgtaa taactctttt gcggttaata cattctcatc tataatctta tctttttgtt
     2521 cttgtatata ttgcttgatg tgcggctttt tcaataacct acacgctgtc acatgtgcgc
     2581 tatttgcgtt atatcctgct tttatggcac tttgtgttac attcagtgtt ctaatatact
     2641 cattcacaaa acgtgcttgc tttgcagtta actcactcat tttatcaccc ccacaatttt
     2701 atctaatatg gtttcatacc ataatattac agattgttct gaacaatcta aggcactact
     2761 aatatcttga taactaagtc cttgtataag ggagtcaaaa atataaaact ctttatcggt
     2821 cgctaatctg tcaacaatca tttctatgtg attctttata atatgatcat tgacattatc
     2881 gtctgtcatc aattcgtcag aatcttcatc acctattaaa aagaaatcat cagtatttat
     2941 ttcatcatcg ccccgttaac tagctttgaa gtctttagca cacttgcata taccggctgt
     3001 cgtgctggcg agatactagc attgaagtgg tctgatattg attttgaaaa caacacgatt
     3061 agtattacta aaacatatta caatccaaat aataacaaga agaaatatca gatacttccc
     3121 cctaaaactg aaagttctat cggtaagatt tccgttgacc caaatgtaat aaaggtgttg
     3181 cgtgattata agataaacgt tcaaaataat tggaaaaacg aattatataa tgataaccat
     3241 tgtaatgaaa aaactatcaa tgtggataca aaccattatg tcacgtacca gcatactcaa
     3301 aaatatctct acgcactcat tcagatacac acattgcgca ttgctcatag aatcaggtgt
     3361 acacattaaa gaaatacaag aacgattacg gcataaagat atcaatacca ctatgaatat
     3421 ctatgctaaa atcacaaatt catacaaaaa agacgcctcc cataagttta gtcaccaaat
     3481 ggaagacgtc tcgtaattaa aatatatttg cgtgcattct aatttatact tagaatgaat
     3541 catactcgtg cataatgtaa ttttctagtt agtcaaaact ataaacagtt ttacatcatt
     3601 cctggcatgc cacccatgtt aggttggtca ttattttttt ctggaattga tgctacaacc
     3661 gcttcagtcg ttaagaacat tgctgcaaca cttgcagcat gttgtaatgc tgagcgtgtt
     3721 actttagttg gatcaacgat accttcttct aacatattaa cccactcgtt tgtagcagcg
     3781 ttaaaaccaa cacccggctc tgcgtttttc aaacgttcta caataacaga accttctaat
     3841 cctgcatttt cagcaatttg acgaactggt gcagttaatg ctttaagtac aatatttaca
     3901 cctgtttcaa tgtcaccttc agcttcaatt tcacttactt tttggtaaac atttactaat
     3961 gcagtaccac cacctgcaac aataccttct tcaactgctg cacgtgtaga atttaatgca
     4021 tcttcaatac gtaatttacg ttctttaagc tctgtttcac ttgctgcacc tactttgata
     4081 actgcaacac cacctgctaa tttagctaag cgctcttgta atttttcacg atcaaagtca
     4141 gattcagttt cttcaatttg agatttcaat tggctaacac gtgcatcaat gctgttttcg
     4201 tcaccgtcac catcaacaac agtggtatta tctttagtta cttctacttt acttgcagta
     4261 cctaacatat caattgatgc atcttttaaa tctaagccta aatcatcagt aatcacttgc
     4321 gcaccagtta aaatagctaa atcttcaagc atcgcttttc tacgatcacc aaaaccaggt
     4381 gcttttactg caacagctgt aaatgtgcca cgcatacggt ttagcacgat atttgttaat
     4441 gcatcgcctt caacttcatc agctacaatt aagattggac gattagattg aaccacttgt
     4501 tctaataaag gtaagatatc ttggaaagac gagattttct tatctgttac taaaatgtat
     4561 gggcgttcta attcagcaac cattttatct gaatcagtaa ccatatacgg tgattgataa
     4621 ccacgatcaa attgcatacc ttcaaccact tctagttcag tgtttagtcc atttgattct
     4681 tcaattgtaa tgacaccatc gttacctact ttttccatag cttcagaaat ataacgtcca
     4741 atttcttcat ctgctgctga aatcgcacct acttgcgcaa tttcattttt attttcaact
     4801 ttttgagaat tttcatgtaa cgcttcaaca gcaactttaa ctgctttgtc gataccttgt
     4861 cgtaaaccaa ctgggttcgc accacttgta acatttttca agccttcttg aatcattgct
     4921 tgagctaata ctgttgcagt tgtcgtaccg tcaccagcaa tttcatttgt cttatttgcg
     4981 acttcttgaa ctagtttagc ccccatattt tcatatggat cttctaattc gatttcttta
     5041 gcaatcgtca caccatcatt cgtaattaaa ggtgctgtaa actctttatc taatacaaca
     5101 ttacgtcctt taggaccaat cgttacttta actgcatttg caagttggtc aacaccacgt
     5161 aacattgctt gacgtgcatc ttcagagaat ttcaattgtt taaccataaa tgataaacct
     5221 ccatttgttt agttgtgttt cattttcgtt ctatttaatt tatttatgaa ttaagttctg
     5281 tattattcaa taactgctaa aatatcttct tcatttaata ccagatatgt ttcattatct
     5341 cgtttaactt ctgtaccagc atattgttgg aacacgacac ggtccccttc tttcacttca
     5401 ggagtcactc ttgtaccatc atttaatagg cgtccagttc ctactgcaac gataacgcct
     5461 tcgtttgatt tttctttagc actatcagtt aaaacaatac cacttttagt tgtttgttct
     5521 tgttcttttt tctcaataat cacacgattt ccaattggtt ttagcatgat tgttcctcct
     5581 taaaaaacct aaagtttagc acttaacatt aaagagtgct aacatacatt tataataatc
     5641 aaatttggtc aaaatttcaa gtcataacct tttaattaag ttttgtaact tcaagttatt
     5701 tacgataaaa taaattataa acaaatattt ggaggaaaat tatgacaaga ttatgggcat
     5761 cattgctaac tgttattatt tatatattgt ctcaattttt accgcttctc attgtaaaaa
     5821 aattaccatt tgtacaatat agtggcatag aactgactaa agcagtcatt tacatacaac
     5881 ttgttctatt tttaatcgcc gccacgacga ttattttaat taatttaaaa attaaaaatc
     5941 caacaaaatt agaattagaa gttaaagaac ctaaaaaata tatcattcca tgggcattgc
     6001 ttggatttgc attggtaatg atttatcaaa tggtagtgag cattgtatta acgcaaattt
     6061 atggtggaca acaagtaagt cctaatacag aaaagctaat tattattgct cgaaaaatac
     6121 ctatatttat cttctttgta tctattattg gtcctttatt agaagaatat gtattcagaa
     6181 aagtaatctt tggagaatta tttaatgcga ttaaaggtaa tcgtatcgtg gcatttatta
     6241 ttgctacaac agtaagttca ttaatatttg cattagcaca taatgatttc aaatttattc
     6301 cagtttattt tggtatgggt gtcatttttt cattagcata tgtttggaca aaacggcttg
     6361 ctgttccaat tattatccat atgttacaaa acggatttgt cgttatattc caattactta
     6421 atcctgaggc tttgaaaaaa gccacggaac aagcgaattt tatatatcac atttttattc
     6481 cataataaaa aaaacgaatc acacacggat tatcgtcgct gtgattcgtt tttatttttc
     6541 tttttatttc gctttacaaa taagaatagc atgattgaaa ataccaccat catagaacat
     6601 acaattgcta gccaactttt actatatgca gttgacgcac ttttcttatt atcttcttgt
     6661 tttttaacta ctgtaaaatc gtctttttca tgcggttcaa atgagcgttg ttttgaatcc
     6721 ggcttatctt cgggcttttt caaatctggc attgatccaa tttcaccagt aattaatgcg
     6781 agtacttgtt catctaaatt acgatagtac ctttctgatt tcaatggatt gaaatattgg
     6841 aatctaaagt aattcgattg ctgttgtaat ccatttaaat atgcatttcc gctgaaattt
     6901 tgttttcgaa taatattata aatgtcttca tcagtaactt ctccataatc tttgcccaac
     6961 ttattaattt gatttaaaat atacggatta tacttgtaag ccccatttgc caaggctaaa
     7021 aatcgttggg atacaaaatc attaccagta ggattttgtg agtttccttg attcccattt
     7081 ggttgccatt gaccttgatt agatccatct gaagcatttg ggttccatgt ccccccattt
     7141 ttcgagccac cagaatgatt ggaatcccca ggttgatcgg gatctggtga cgggttagga
     7201 tttggcttat tagggtctgg ttttggattc ggatttggct ttggtttatc tggatctggt
     7261 tttggatccg gatttggctt attagggtct ggttttggat ctggatttgg tttcgggtta
     7321 tctggatctg gttttggatc cggatttggc tttggtttat ctgggtctgg ttttggatcc
     7381 ggttttggtt tcgggttatc tggatctggt ttaggattcg gacttgggtt ttggttattt
     7441 gggtctggtt ttgaatctgt acttgggttt tggttgtttg agtctggctt cacattattc
     7501 gaatcaggat tagcatcacg actattcgaa cctgttcctt cattacccgt ttgatttgat
     7561 tcgtgaggtg tcccagtctc attattattc atttcactat ttactgatgg tgaagttata
     7621 ttatgattca aattagtagt tggtttttct ccatttaagt tatcctttgc ttcaactgtg
     7681 tgcgttgcaa gccctaaact actactactt aaaattaaaa tacttgttga aagtaacatt
     7741 ttcttaaacc aatgatatga catttcacga gacacctccc tttttcatgt aagtattaat
     7801 ctaagtataa taaatatgcg ttgaaaatgc atttatattt ggaatttgaa aatgaatatt
     7861 aatattcttg aaattttcgt aaatattgct ttacaagtac gagtataaat tttatactta
     7921 gtctattaag tattctttac taaagaagga gcaataatta tgggcttatt taaaaaagat
     7981 aaaaaagcaa tgacttttga caatgcaatg gaagaaagac gttcaatata taatttgaaa
     8041 gattcgatat caattagcga tgacgaatta gaatcaatga ttgcacatgc agttaaacat
     8101 gttccttcat catttaattc tcaatcaact agaattgttc tcttgttaaa tgacaacaat
     8161 aataaatttt gggataacac aaaagcaatt ttaaaagaag tcatgggaga gaatcgtgat
     8221 ttcgagccaa ctgaacaaaa aattgataac ttcaaacatt catacggtac aattttgttt
     8281 tatgaagatc aagatgttgt aagtggttta caagaacaaa tgccaaacta ctatgataat
     8341 tttgcaatat ggtcaactca aacaaatgcc atgcatcaat ttgcaatttg gactgcatta
     8401 gctactaaag gcataggtgc ttcattacaa cactataatc cacttgttga tgagatgact
     8461 tcaaatgaat ttaatattcc taaaagttgg aaacttattg ctcaaatgcc atttggtgat
     8521 attcgtgaag ctgctggcga aaaaacattt aatccagtgg aagatcgttt cgtaataaaa
     8581 aaataatcaa tatattgtta ggaatgggac agaaatgata aagaatcatt agttattcat
     8641 tatgtagtag ttcttacaca ttagccgctg ctaatgtgca cttaagaata ggaatacatg
     8701 agtaaaactc atgtataaga aatactaatt tctaaagaaa aagtatttct ttatgttaga
     8761 gcccacccca acttgcattg tctgtagaaa ttggtgttcc aatttctcta tgttggggcc
     8821 ccatcaacta ctgtcaatat aactttgtag agcctaggac tttgatttta tgtcttgtgc
     8881 tcgttttttg cgtttcaaca taacttttag taagttatat ataagatcta tcaaggatgt
     8941 gatgttatga aagtccaaat ttatcaatta ccgattgttt ttggagatag ttctaaaaat
     9001 gaaactcaaa taacgcaatg gttcgaaaaa aatatgaatg ctgaagtaga tgtagtcgtt
     9061 ttacctgaaa tgtggaataa tggctatgat ttagagcatt taaatgaaaa agcagataat
     9121 aatctaggac aaagtttttc atttattaaa catttagctg aaaaatataa agtcgatatc
     9181 gtagctgggt cagtttctaa cattagaaac aatcaaatat ttaatacagc ttttagtgtg
     9241 aataaaagtg gccagctgat taatgaatac gacaaagttc atttagttcc tatgttaaga
     9301 gagcatgaat ttttaaccgc tggcgaatac gtagctgaac cctttcaatt gtctgacggt
     9361 acttatgtga cccaacttat ctgttatgat ttacgttttc cagaattatt aagatatcct
     9421 gctcgtagtg gtgctaaaat tgcattttat gtggcgcaat ggcctatgtc acgtcttcaa
     9481 cattggcatt cattactaaa ggcacgtgca attgaaaata atatgtttgt cattggaact
     9541 aatagcactg gattcgatgg taacacagaa tatgctggtc attcgatagt tataaaccca
     9601 aatggagatt tagttggaga attaaatgaa tccgcagata ttttgactgt agatttaaac
     9661 ttaaatgaag tagaacagca acgcgaaaat atacctgtat ttaaaagtat taaattagat
     9721 ttgtataaat aagaagcgcc cgaaataata tttaacacat gtcatgacta aacatagatt
     9781 tatgagagat ttttcataag tctatgttta tttttttata atagataact cagtaagaac
     9841 ccatttcgcc ctttgcaaat gaatgatttt cttaattaaa ttgattaatt ttttgacctt
     9901 ttccaacatt agacttattc atatatttta acggcgggtc tcataatgat gattaactca
     9961 tcccttcttc attacaaaaa aggccgcgag cttgggaggg gctcacgacc atacttatta
    10021 ttaagggaat gttttacagt tattttttca atctattttt ggggatgtta ttaattatga
    10081 aaaaaatttt atttatcaaa taatgataaa tcgatgttgt ttacgatagc ttacatgcta
    10141 gaaataatta tctgtaatga taattaagaa aaatacatag cactgagtcc aaggaaacta
    10201 actctactag caaatgttac tcacttgcta attttacatg gttattaagt tgggatggct
    10261 taataactca tacttttagt actatacgaa gataacaaat ttacaatgaa agtaattaat
    10321 tattcatctt attttttagt gaatttgttc actgtgtcga taatccattt tactaagtca
    10381 ccgattgttg aaatgatatc ttgtgccatt gaaatcactc cttccttaat taagataaaa
    10441 attcttaaaa ttaaacaact catcaactat tttccatcac atctctgtga tctagttata
    10501 ttaaaacatg ctaaaagcat ttattttcca atttttctta actagtcgtt ttttattctt
    10561 aactgtaatt ttttttatgt taaaatatta aatacaaatt acatttaaca gttaagtatt
    10621 tatttcctac agttaggcaa tataatgata aaagattgta ctaaatcgta taatgacagt
    10681 gaggagagtg gtgtaaaatt gaattatttt gataataaaa ttgaccagtt tgccacgtat
    10741 cttcaaaaga gaaataactt agatcatatt caatttttgc aagtacgatt agggatgcag
    10801 gtcttagcta aaaatatagg taaattaatt gttatgtata ctattgccta tattttaaac
    10861 atttttctgt ttacgttaat tacgaattta acattttatt taataagaag acatgcacat
    10921 ggtgcacatg caccttcttc tttttggtgt tatgtagaaa gtattatact atttatactt
    10981 ttacctttag taatagtaaa ttttcatatt aactttttaa ttatgattat tttaacagtt
    11041 atttctttag gtgtaatctc agtatatgct cctgcagcaa ctaaaaagaa gcccattcct
    11101 gtgcgactta ttaaacgaaa aaaatattat gcgattattg ttagtttaac ccttttcatt
    11161 atcacactta tcatcaaaga gccatttgcc caattcattc aattaggcat cataatagaa
    11221 gctattacat tattacctat tttctttatt aaggaggact taaaatgaat acattattta
    11281 acttattttt tgattttatt actgggattt taaaaaacat tggtaacatc gcagcttata
    11341 gtacttgtga cttcataatg gatgaagttg aagtaccaaa agaattaaca caattacacg
    11401 aataatttaa atagagagtg tgatagtagg tggaattatt aaatagttat aattttgttt
    11461 tattcgtatt aactcaaatg atattaatgt ttacaatacc agctataatt agtggtatta
    11521 agtacagtaa acttgattat tttttcatca tagtaatttc gacattatcg ttatttctat
    11581 ttaaaatgtt tgatagcgcg tccttaatca tattaacttc atttattatt ataatgtatt
    11641 ttgtcaaaat caaatggtat tctattttgt tgattatgac ttcgcagatt attctatact
    11701 gtgctaacta catgtatata gttatatatg catatatcac caaaatttct gatagtatat
    11761 ttgtaatatt ccctagcttt tttgtagttt atgtgactat tagtatacta ttctcatata
    11821 taataaatag agttctcaaa aaaattagca caccatatct aatactaaac aaaggatttt
    11881 taatagttat ttcgactatc ttactgctta ctttttcatt atttttcttt tattcacaaa
    11941 taaactcg
//
LOCUS       DAKTGS010000087        11915 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.087, whole genome shotgun sequence.
ACCESSION   DAKTGS010000087 DAKTGS010000000
VERSION     DAKTGS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.087"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            1..407
                     /locus_tag="O0286_001879"
                     /pseudo
     CDS             1..407
                     /locus_tag="O0286_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(515..1180)
                     /locus_tag="O0286_001880"
     CDS             complement(515..1180)
                     /locus_tag="O0286_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170253.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(1180..2235)
                     /locus_tag="O0286_001881"
     CDS             complement(1180..2235)
                     /locus_tag="O0286_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170254.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            2371..3045
                     /gene="hssR"
                     /locus_tag="O0286_001882"
     CDS             2371..3045
                     /gene="hssR"
                     /locus_tag="O0286_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCY0170255.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            3038..4411
                     /gene="hssS"
                     /locus_tag="O0286_001883"
     CDS             3038..4411
                     /gene="hssS"
                     /locus_tag="O0286_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCY0170256.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4574..5017
                     /locus_tag="O0286_001884"
     CDS             4574..5017
                     /locus_tag="O0286_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0170257.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            5014..5469
                     /locus_tag="O0286_001885"
     CDS             5014..5469
                     /locus_tag="O0286_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCY0170258.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5535..7013)
                     /gene="mqo"
                     /locus_tag="O0286_001886"
     CDS             complement(5535..7013)
                     /gene="mqo"
                     /locus_tag="O0286_001886"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0170259.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(7394..8992)
                     /locus_tag="O0286_001887"
     CDS             complement(7394..8992)
                     /locus_tag="O0286_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0170260.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            9309..10982
                     /locus_tag="O0286_001888"
     CDS             9309..10982
                     /locus_tag="O0286_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0170261.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(11135..11764)
                     /locus_tag="O0286_001889"
     CDS             complement(11135..11764)
                     /locus_tag="O0286_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170262.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
BASE COUNT         4047 a         2089 c         1701 g         4078 t
ORIGIN      
        1 ttaattccat ttttaacaat gattcaaaat accttcggtt atttaccgga gtatattgta
       61 gctgatgcat gttatgatag tgagcaaaac tatatggcta ttatagatga ttttaataaa
      121 acgccactta ttacgtatgt catgtttatt aaagataaaa ctagaaagtt taaaagtgac
      181 atttttaaca ctcaaaattg gaaatatgac gaacttaatg atgaatttat atgtcctaat
      241 aacaaaagaa taggttttaa aagatatgca taccataatg atagatatgg ttttaaacgt
      301 gacttcaagc tatatgaatg caacgagctg tacattataa aatacatatc aaaaaagctg
      361 atttctatca aataattaat agaaatcagc ttttttacat tgcctaagaa cttaatgtcc
      421 cagcctgttt tttgtaaacc caaatcgtat tctgttatct aacttttata ctactatgtc
      481 ttaaatcatt aacatttagc gtctttacaa gccactaatc agttattttg ccatcttcta
      541 attcaatcac tcgatctgca tattcaaata atcttcgatc gtgtgtaatc ataataccaa
      601 tcatttgttg ttctttaatt tgttgacgta tcatctcaac aacttttgtt gctctatcgg
      661 catctaaact tgctgtgggc tcatctgcta aaatgatttt cggattattc ataaatgctc
      721 tcataatcgc aacacgttgc ttttcaccgc cagataactg atgcggatat acattcaagc
      781 gatcttctaa accaatattt ttcaaaagtt gtattgctct tgttgaactt tgttgtttgg
      841 tcattcccgc ttcttgacct acgagtgtca attgctctat cacttttaaa taaggaacta
      901 aatgtgaaga ttgaaaaata aaaccaatat cttccaatcg taaatcacta ggacgatgct
      961 gtttatcaaa caatggcgca tcattgtaaa gcactgtacc actcgtttga cttaacaatc
     1021 cgcctaatat cgttagcaat gttgttttcc cagaaccaga ggcaccattt aaaatgacaa
     1081 attccccttg ttccacttca aaattaatac cttttaaaac ttttgtttca gacaaacctt
     1141 ctccgaaatt tttgacgata tcttcaacga ctaatgccat tattctgcac ctccaattgc
     1201 ttcgatagga tccactttaa ataatttgat aaatgatagt gaggcaccta aaatcgctac
     1261 taatataaat atccccacca ttaataaaat attttgcgtt gttaagtaaa aaggcatcgt
     1321 taccggcatc ataaatgata gtcctactat gatgatcaca gcaataccta ccccaattat
     1381 tgttagtgtt aaaatttgta aaactaacgc actcaataaa tgtcttgtct taataccaat
     1441 tgctttcaaa atgccaattt gtgatatttt ttgaatcgtc ataacataga aaaatgcact
     1501 tagaacgatt gctgtaatag caaacaaact aacaatcatc atatttaacg gtgcttgctc
     1561 tgcttgataa ctcgcaatat tacctgttaa atctttttca ctcacaactt gaacgtcatt
     1621 tattttatta agctcatcac gttgttgttg tgttaaattt ttcactggat aaaatgccgt
     1681 attaacctta ttcaatgcat taaatccgtt atcgttcaat agcacaatgg aactatgcgc
     1741 atacattgtg tcgtttaaaa taccagaaac tctataacta gttgaattat ttttaaaatg
     1801 taatctatcc ccgactctaa tgttgtctgc agctaactta tcattaatag cgacctcatg
     1861 agcattttta ggataatcac cttttttaag ttgtatgcgt tgttgtttaa cgtcattcat
     1921 tgtaatcaca tcttgttctt tattagataa tttcaatgtt tgaatattca ttttatatgg
     1981 ttcttgatga attacctttt taatttgatt ctgttgcgta tcactaagtt gcgatttctc
     2041 aatttgcggc tctttcattt tttgaacaac atattcatca ttatcaaagt gttcaaataa
     2101 cgaaatattc tccctaccaa gcccttgagc taaaccacta ataaataaca ccataatact
     2161 taataaaaga atgattaatg tgattaaaat ataacgaaat ttgtaaaaca taatctcttt
     2221 tatcgctaat ttcatatcga ttcacttctc cctatttctt ctttagcgtc agtataaggt
     2281 tgaaatatga actcaatatg aacaattgtt tttttacgca aaaatatttt acatgtataa
     2341 taaatgaaaa agataaggga gtttatagct atggtgcaat gtcttgttgt cgatgacgat
     2401 cctcgaatcc taaattatat agctagccat ttacaaacag agcacattga tgcatacaca
     2461 caaccaagtg gtgaagctgc attaaaactt ttagaaaaac agcgtgtcga tattgcagtg
     2521 gtagatatta tgatggatgg tatggacggc tttcaattat gtaatacatt aaaaaatgat
     2581 tatgatatac cagttattat gttaacagcg cgggatgcac ttagtgacaa agagcgtgcg
     2641 tttataagcg gtactgacga ttatgtaacc aaaccctttg aggttaagga acttattttt
     2701 agaattcgtg ctgtattacg tcgatataat atcaattcaa attcagaaat gactattggc
     2761 aacttaacgc taaaccaatc ctatttggaa ctccaagtat ctaataaaac gatgacgtta
     2821 ccgaacaagg aatttcaatt attatttatg cttgcagcac gtcctaagca aatctttact
     2881 cgtgaacaaa taatagaaaa aatttggggc tatgattatg aaggagatga gcgaacagtt
     2941 gacgttcata ttaagcgact acgccaaaga ttaaaaaaat taaatgccac acttacaatt
     3001 gaaacagtaa gaggacaagg ctataaggtg gagaatcatg tttaaaacac tctatgctag
     3061 aattgcgatt tattccatta cggttatttt atttagtgca ttaataagtt ttgtattaac
     3121 aaatgtttac tatcattata atttaaaagc atctaatgac gcgaaaatca tgaagacgct
     3181 taaagaagca agacaatatg aacaatcagc taaaccaacg cacattcaac aatatttcaa
     3241 acatttaggg caaatgaatt accaaataat gaccattgat caaaaaggtc ataagacatt
     3301 ttatggggag ccatttagag aagatacttt atcacaaaat gctattaaca acgtattaaa
     3361 taatcaagat taccatggta ttaaagataa accattcgca ttattcgtaa ctggattttt
     3421 cgacaatgta acagataata ctgttggtat taactttaag acaaaagacg gttcaatagc
     3481 agtatttatg cgtccagata ttggagaaac atttagcgag tttagaacat tcttagccgt
     3541 cttgttaatg ttgttattat ttatctcgat ttcattagtt atcgcatcaa cctattcaat
     3601 cattcgtcca gtaaaaaagt taaagctagc gaccgaacgc ttaattgatg gtgattttga
     3661 aacacctatc aaacaaacac gcaaagatga aattggaaca ttacagtatc actttaataa
     3721 gatgagagag tcattgggac aagttgatca aatgaggcag cattttgtac aaaatgtatc
     3781 tcatgaaatc aaaacaccat taacacatat tcatcattta ttaagcgagt tacaacaaac
     3841 ttctgataaa acattaagac aacaatatat aaatgatatc tataccatta caacgcagct
     3901 gagcggatta acaactgaat tgttgctctt atccgaatta gataatcacc aacatttatt
     3961 attcgatgat aagattcaag tcaatcaact cattaaagat atcattcgac atgaacaatt
     4021 tgctgctgat gaaaagtcgt taatcatttt agctgaccta gaatcaatca atttcctagg
     4081 aaatcaacgt ttgttacatc aagcattgag taatttatta ataaatgcta ttaagtatac
     4141 agatgttggt ggtgccattg acatcgcttt gcaacatagt cacaacaata tcattttcac
     4201 aataagtaat gatggttcac caattagtcc acaagctgaa gcacgtttat ttgaacgttt
     4261 ttataaagtg agcaaacatg ataatagcaa tggtttaggt cttgcaatta caaaatccat
     4321 cattgaattg catcatggta caatccaatt cacacaaagt aacgagtatg taaccacctt
     4381 tactattaca ctgccaaata attcacttta atctctacct cctgaaaaaa catttctgtt
     4441 tttatttcag gaggtatctt ttatattgat tagcgctttc tagccactta acatttaatt
     4501 tctaccattc aacaaaattt aaatatatcg atggtcattt atggtttaat aattttgaag
     4561 gaggcattaa cctatgatga aactcaattt atttatcaat gcaaatgaga cggaatctta
     4621 tattgatata catgcaccta aaatgaatga taacgttcaa agtattatta atgcagtcaa
     4681 tgatttagac aaatcacata cattagtcgg atacatagac aaagaaatcc atatcattaa
     4741 tgtatcagat gttataacat tccaagttat taataaaaat gtaacagcca ttacaagcaa
     4801 tcaaaaattc aaactaaaat tacgacttta tgaactagaa aaacagttac cacaacattt
     4861 cattcgcatt tccaaatcag aaatcgtcaa caagtattac attgaaaaat tattattaga
     4921 gccaaatgga cttatacgta tgtatcttaa agatgcacac tacacctatt cttctagacg
     4981 ttatttaaaa tctataaagg agcgcttatc aatatgaaaa atttgaaaaa ctcattattt
     5041 attagcttaa tcattggttt gtccctttca ctgttcttta gtatgttatt tgcagacggc
     5101 aaatattatc cactgaaccc acaatcaact attggcatat tgtattatac gcatttcaca
     5161 gaaactaccg taatgttgat ttctatcatt ttatggttac tcataggcgt cgtctttttc
     5221 cttggagatt ttatctttaa atacacagat tggagcatta ctaaagcaac tataatgcat
     5281 ttcataacaa cgtatgtcgg attcttgcct ttagcaatgc ttgccggttg gtttccacta
     5341 acagtgcatt atctaatcat attcacaatt atcttcattg tggtttatgt attaatttgg
     5401 ataattcaat tctttaaaaa caagaactac gtagacacta ttaacaagca actgaaacaa
     5461 ttaaaatgat ttttattaac cactaaaaaa cggtaatgtc tatcacatta aaaaagacat
     5521 taccgtttcg tttattattt aacttgtaaa tacttagtta cttcttcatt aattttatga
     5581 tataaatcct catgatctgt taagcgataa ccaaatgacg gcaccatttc tttaatcttt
     5641 ggttcccatc ctttgaattc atcacgatag catctctgta aaacatcaaa cataatatct
     5701 acagctgttg acgcaccagg tgatgcacca agtaatgcag ctaatgtgcc atcatctgac
     5761 gtaataactt cagtaccaaa ttgtaagtta cctttagaat cctcagtatc cttgattact
     5821 tggacacgtt gccctgctgt aatcacttcc caatcttcat ttttagcatt tgggaaaaag
     5881 actcttaaat catccatacg ctcatcatta gataacatca attgtgacac taaatacttc
     5941 gtaagactca tttctttgat acctgcagat aacatcgtca cgatattatt tggtttaacc
     6001 gatttaatta aatccatatg tgaacctgtt tttaaaaatt taggtgagaa acctgcaaat
     6061 ggaccaaata acaatgaacg cttgccgtct acaaaacgtg tatctaagtg cggcactgac
     6121 attggtggcg cacccactgc tgcttttccg tacactttag catgatgacg atcaatcact
     6181 tcttgatttg tacagcgcag gaataatcca cttaccggga aaccaccaat atgttttgat
     6241 tgtttaatcc cagtcttttg gagtaatggt aaactcgcac cgccagcacc aataaataca
     6301 aaatcagatt catatgttgt cgtttcatta gtttctaaat ctttaacttt aactagccat
     6361 accccagatt tctgtttctt gatgtctaat acattctgct tatattgaat tcccacacca
     6421 cgttgctcta aattatcaaa taactttgca gttaacgcac caaagttaac atctgtacca
     6481 gtttcatcat aagttaaagc aattggttca tcagacttac gaccttcaat cattaacggt
     6541 acccaagatt tcatttcttc ttcatcttgc gataatttca ttttttcgaa taaaatactt
     6601 ttctttaacg ttgcaacacg actttttata aaagctacat tatcttcgcc aatgacaaaa
     6661 ctcatgtgtg gcaccgcttg aataaagcga tctgggttat ctaattgtcc tgttttaact
     6721 aaatatgccc aaaactgttt tgaaatttgg tactgctcat ttatcttaat tgctttacta
     6781 caatcaactg tgccatcctt accttctttt gtatagttca actcgcaaag tgctgaatgc
     6841 cctgtaccgg cattattcca tacatttgaa ctctcttcgc caggttgtgc taatttttca
     6901 aacactttaa tatttttctc aggtgataat tctttaagta atgttcctaa tgttgcactc
     6961 ataataccgc caccaattaa gatgacatct gttttgctat gttgtgttgt cataacaaat
     7021 acagtccccc tttttaaaat gaataatgga acgttcgaga aatacgacat attaatacca
     7081 ctttaaatca ataagtgtct caataggttc ccatggtgat gctctattac tatctactat
     7141 gtatattaat attttataat aacgaaacta ctttaattat atactttatg tgcgatagca
     7201 caaagacaaa accacaaacc ttaacagcta tcaaccaatt taatttgatt gctgattact
     7261 cactattcat tttaacataa agcgctacca ttattataat gtcaaacttc tttcattaat
     7321 gatccataaa aaaaagatga gatgttagaa aacatctcac cttctgtata tttatatttt
     7381 gaaatacgtt tttttataat aatgacaaga tgaaagtcca aatacagatg aatattagta
     7441 aacatacact gtatttcaat gtcattttta ataattctga ttccttacca acttgtttta
     7501 ctgctgcagt tgcaattgca attgattgtg gtgaaatcaa ttttgccgct acaccaccaa
     7561 ctgtattagc agatacaaga agtgaaccac ttgttccaat ttgttgtgca acagaagctt
     7621 gaattggtgc aaataatgag ttgttattta caactgatcc tgtcataaac acacctatcc
     7681 aacctaaaat tggtgataga actgggaaga cattacctgc tttagcaata ccttgaccca
     7741 ttgctgcact taaaccacca taagttgtga ttttagaaat tgctaagatg aaacaaattg
     7801 taagaactgg taaccacaac tctttaaatg taacgccgaa taatctacct gcatctttaa
     7861 agttaacctt tttagacatt aaaattgtaa taataatagt taataaaata gctgtacctg
     7921 tttgtccaat aatatttaaa gtcaatacta atggtttatg cgtaacttcg ctgattgttc
     7981 caggtaagtt gaatttaaat actaatgatg ataaagcacc atttggtaag aataagtttt
     8041 taaagaatgg cgcactccaa atcattacaa tgactgttaa tacaatgaat gggctccatg
     8101 catgtaatac tgcttttgca gaatgtgctt ttgcaggttc aatttcttca tctttattaa
     8161 cacgataaat gtgttttggt tggaattttt tagaaaatac tgctaatgct aacattgtta
     8221 ataacggtgg aataatatct gctaattcag gtccactgaa tacagttaat aatccttgag
     8281 taaatgtata agtgattgaa actactaaaa ttgctggtaa tgtttcttta acacctctaa
     8341 aaccatcaat aataaagata agtaagaatg gaataatgaa atttatgatt gccaatgtta
     8401 aagttgctga ttgagaaaca cctaatactg aaacatctcc aggtaatttc aacgtttcta
     8461 caacacctac agggatacca atcgcaccaa aagcaccaga cgctgcattt gcgactaaac
     8521 ataacatcgc agcttttaat ggattaaatc ctaattgtgt taataaaagt gcacaaattg
     8581 caattggtac accaaatcct gctgcacctt ctaaaaatgc gttgaatgca aatccaataa
     8641 gtaaaacttg aatacgttgg tcttgtgaaa tatttgtaat actatcttga attgttaaaa
     8701 attgtccaga ttcaacagta attttgtata acaatactgc catcataacg atatagccaa
     8761 tcggaatgat accttggaag aaaccttcga ctactgcacc agaagcgata ccaactggca
     8821 atttgaaaaa tggtattgca attagtaatg ttacaacaag tgttgtaata gctgcataaa
     8881 tacctttcat tttaaaaaca gttaaacata gtaaaaacag tacaatcggt attgctgcaa
     8941 ttaaagacga taataataaa ttatcaaatg ggttaaacgt atttactaac ataggctgac
     9001 tcttctttcc ataaagtagt agtgtaattg cttcattaaa atcatgctct ggctatagca
     9061 ttttgttatc gataacattt tacagaacag tgagattaat gtgatttttt tcacaattaa
     9121 aatataccat tttttcagac tattaacaat atcactttcc tatttaattg ttttattttt
     9181 cattttaaat ctgttcatca aaatgaattg tacttaatgt aatgaagtaa cttatactat
     9241 tcatgaattt tacataagac cgtgctaaaa taaggaagat actacgatga ggaatggaag
     9301 ttatagcaat gattaaacag ataaatgttt ctaatatgca aaagtttgaa tctcaattaa
     9361 tgaaagctca gtctgaaggt tatacacatg tcgttccata tgccaatgag ataatgattt
     9421 atcaatcgat gttagatgcg gttcaattat atcctaagtc tatcgtggtt gattatactg
     9481 tggatggaca atacaaaaat gattgtcact atttcggtca atcatcgatc aacattgccg
     9541 attgggctca aaacaataat tattatccta acctgatata tgcgattcaa cagactcttg
     9601 atttaataca ttattactct gtagaaacga ttttcgattt agctttattg acacttctga
     9661 aaggtgattt atcaatcgat ggtcatgtcg tttttgattt taaagcccct atagcaacaa
     9721 gtgcttcaat atgggaaact attaaaacta ttgaagattt tgatatgatg tctcagtttt
     9781 atttgaataa aatggcttat attgatcatc atcctatacc ttttcgcaac ctttttatcg
     9841 aggattcaga acaacttaat tcgccagata attggttgta ttctaccaag tttatgctac
     9901 ctaaatggtt atataaaata gcaaagcaac gtgctgataa caaacaatta caaaacttcg
     9961 gcctgtatac gaagcaacca aacgttttaa aagatcacat cgtttttatc ggtgatcatc
    10021 atcaatatat aggtaattca aaatatttat ttacttactt cgttaaacat aatccaatga
    10081 ctgcctgtta ttttgttact gacgatcgtc gcggaccaca tttcatatca cctaagtctg
    10141 aaaaagctga cgaattgatt aacagcgcac gcgtagttat agttgaaaat gatatacctg
    10201 aaacgttaca accaaacggt actttaattc aattacatca aggcacacca ataatgcaat
    10261 tatttttaga tagtaaagag cctattaaaa atatagaaac acctttttat cgtgctaaaa
    10321 ggtataatcg ctggttgcaa ttcgattatg ttatacattc tgctgatgat attagccact
    10381 tttatcaaac ggcttttcct agtcaccaag caaatgtatt ggcttatggt aatcctaaac
    10441 accaatattt actacaaaag agtaacgaaa gtactactca acaacagtat aagaaatcgt
    10501 ttaaaataaa tgatcaaaaa ccagttttat tatacgcacc tattggatta gtgagtgcgc
    10561 aacaattacc actatcagat gctttattca aagcctatca tgttgtggtt cagggtgttg
    10621 atgaagcgat cttaccagaa gaggcgcttg ttgcaccaaa gtatcttagt gctcaagact
    10681 taattctaat gtctgatgtc gttatcactg attactcaaa tataatattt gatgcgatgg
    10741 ctatagacaa aacagtagca ttatacactc caaatcacag tcaatacatt gagtctcaag
    10801 gtgttaacga agatatttgg cgtcatctat caaaaatttg gtatacagac cgacaattat
    10861 taattaataa tttaatttcc caggcaatac cagtgattaa atatccacaa atacaacata
    10921 aagaacaacc attagaatct atttcacaac ttatattgtc taagatgaca tctaatcaat
    10981 agtgtttaaa tttctcagtg gctgtgaatg aggtttaaaa gtactataaa acgtaaactt
    11041 tgatacttta aaatacgcaa aaaacggtaa accctaattc atattataga gtttaccgtt
    11101 ttatttttta acttgcatca tagttatatt aacattattg ttggtagttt ggatcagtaa
    11161 ccattgcttg tccagtataa tcaaccgtta caattgaata ttttccattt gcatttgggt
    11221 ctttaaaact aaacacatac ttatagttgc cattatgttc ttcaatagaa taatcattat
    11281 acactttatt attactacca aatttatttg cttcattatt agccgcattt aaagctgttt
    11341 ggaaatttgg caattgctgt aaagcttgat ttttatttcc attaaacgga taaatttgac
    11401 gtgcaaccgg cgcggcattt tgtccataat atggtgcaac gtaacttgat ttttgattat
    11461 tattcgcttg gttattactt gattggttat tatttgtttg gttttggtca ttgtttgttg
    11521 catttgaatt agattgttgc tggttatcgt ttgcactatt atctttatta tctttgttta
    11581 cgtctttact atcatcttta ttatctttct tatctttaga tgaatcattt gtttttttat
    11641 cttgttgttc agttttcgct ttatcatctt tttctttatt accgtctttt tgttggtcac
    11701 tatcttgacc acatgcagct aaaaataatg ataatgctag taaccctgta actaatcttt
    11761 tcatacatat ctcctcctat aattcgatat tcattgaata atcttgaaat acatatctac
    11821 catgtgtatc ttttcatggc ttgttaccaa agacgtcaac attaatatga tctgaattat
    11881 cacaatatcc ataactacaa tctaaggttc ttttt
//
LOCUS       DAKTGS010000088        11900 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.088, whole genome shotgun sequence.
ACCESSION   DAKTGS010000088 DAKTGS010000000
VERSION     DAKTGS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.088"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            311..2122
                     /locus_tag="O0286_001890"
     CDS             311..2122
                     /locus_tag="O0286_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0170263.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            2483..3250
                     /locus_tag="O0286_001891"
     CDS             2483..3250
                     /locus_tag="O0286_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0170264.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            3425..3913
                     /gene="crtO"
                     /locus_tag="O0286_001892"
     CDS             3425..3913
                     /gene="crtO"
                     /locus_tag="O0286_001892"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCY0170265.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            3906..5399
                     /locus_tag="O0286_001893"
     CDS             3906..5399
                     /locus_tag="O0286_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0170266.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            5405..6532
                     /gene="crtQ"
                     /locus_tag="O0286_001894"
     CDS             5405..6532
                     /gene="crtQ"
                     /locus_tag="O0286_001894"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCY0170267.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            6569..7432
                     /locus_tag="O0286_001895"
     CDS             6569..7432
                     /locus_tag="O0286_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCY0170268.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            7444..8952
                     /locus_tag="O0286_001896"
     CDS             7444..8952
                     /locus_tag="O0286_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0170269.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            9379..10533
                     /locus_tag="O0286_001897"
     CDS             9379..10533
                     /locus_tag="O0286_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0170270.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            10556..11554
                     /locus_tag="O0286_001898"
     CDS             10556..11554
                     /locus_tag="O0286_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0170271.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(11644..11850)
                     /gene="copZ"
                     /locus_tag="O0286_001899"
     CDS             complement(11644..11850)
                     /gene="copZ"
                     /locus_tag="O0286_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCY0170272.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
BASE COUNT         4107 a         1740 c         2310 g         3743 t
ORIGIN      
        1 tgattctgtt acagtaatat gcataaaata taaaacgaac cttttttgta aaaatataat
       61 gaaaataaca gttattttgc taaaaattca agaatctatg ataatctatg ttatgtagta
      121 aaatcgacaa atttatatgt ggtttgcagt aatgaaatca actgtaataa ttgttttcta
      181 cttaataaaa aatttaaaac tatcttaatt aagggttaaa tcaattaatg aaaatcagtg
      241 caatatttta ttttcaaaaa tattgtgata tgctacaaaa tagagaaaaa taaatggggc
      301 gtttattaac atggatacaa aagactttaa acgtttagaa aaaatgtatt ccccgcgata
      361 cttacctgga ttagatggat tgagggcatt cgcagttata ggaatcatta tttatcactt
      421 gaatgcacaa tggttaagtg ggggcttttt aggagtagat acattcttcg ttatttcagg
      481 ttatttaata acaagtttgt tgataagtga gtattatcgg acgcaaaaaa tcgatttgct
      541 agagttttgg aagcgacgat tgaaacgact cattccggca gtgttatttt taatctgtgt
      601 cgtgcttacg ttcacactga tatttaaacc ggaattgatt atacaaatga aacgagatgc
      661 tattgcagct atattctatg tttcaaactg gtggtacatt tcacagaatg tagattattt
      721 taaccaattt gctattgaac cactcaaaca tttatggtct ttagccattg aagaacaatt
      781 ttacttgctt ttcccattgg ttatcacgtt cttattacat agatttaaac cgagaaatat
      841 tattcaaacg ctatttattg tatcgttgat ttctttagga cttatgatag tgattcattt
      901 catcactgga gataattcac gtgtgtattt tgggacagat acacgactgc aaactttatt
      961 gcttggttgt atattagcat ttatttggcc tccgtttgct ttgaaaaaag atatttctaa
     1021 aaagattgtc gtatcattag atattatagg gatatctggt tttgcggttc taatgacttt
     1081 gttctttata gttggagacc aagatcaatg gatctataat ggaggatttt acattatatc
     1141 atttgcaact ttattcatta ttgcaattgc ggtacatcct tctagtttat ttgctaaatt
     1201 tttaagtatg aaacctttac taattatagg taaacgatca tatagcttat acttatggca
     1261 ttatcctatc attgtttttg tgaacagtta ttacgtacaa ggacaaatac cggtatacgt
     1321 ttatattata gaaattttgt taacagcgtt aatggctgaa atttcgtatc gctttattga
     1381 aacacctata cgtaaaaaag gatttaaagc ttttgcattt ttacctaaaa agaaggggca
     1441 atttgctaga acagtgttag ttatcctatt attggttccg tctatcgttg tgctcagtgg
     1501 acagtttgat gcacttggca aacaacatga agccgagaag aaagagaaga agacggaatt
     1561 taaaacaacg aagaaaaaag tcgttaaaaa agataagcaa gaggataagc agacagcgaa
     1621 tagcaaagag gatattaaaa agtcatcacc actattaatt ggtgactcgg tcatggtgga
     1681 tattggtaat gtctttacta agaaaatacc aaatgcacaa attgatggta aagttggacg
     1741 gcaactcgtt gatgctacac caattgtgaa atcgcaatat aaagactatg ctaaaaaagg
     1801 tcaaaaagtt gtagtagagc ttggtacaaa tggggcattt acgaaagatc aattaaatga
     1861 actattggat agttttggaa aagcagacat atatttagtt tctattagag tacctagaga
     1921 ttatgaaggt agaataaata aattaattta tgaggcagct gaaaagcgct ctaatgtaca
     1981 tctagtcgat tggtataaag cttctgcagg tcatccggaa tactttgcat atgacggtat
     2041 tcacttagaa tatgcaggta gtaaagcgct gactgatttg attgtaaaaa cgatggaaac
     2101 acatgctaca aataagaaat aatttgatgc actaaacttt tgaaatatta cattacttct
     2161 gatatttatt atcaaaaatg atgtatttca ttaaaagttt agtgcttttt tattttcaaa
     2221 tcccatagta acggtgcaga aaaagtgttg taaacattct aattggtata ttacattcaa
     2281 tgaagcttta ttaggaacag attacattat gataacaaag cccgcaagac acctaatctc
     2341 tgttatagtt tgttttgtcg caaaactata aaagttataa ttgtttgcat actaaaaaaa
     2401 taaaaaatat aaaatttaaa ataattgagt cgctaatgac tatatgtata gagtgttttg
     2461 attattggga ggatatttaa ttatgaaaaa aatcgttaca gctacaatcg ctacagcagg
     2521 acttgcaact atcgcatttg caggacatga tgcacaagcc gcagaacaaa ataacaatgg
     2581 atataattct aatgacgctc aatcatacag ctatacgtat acaattgatg cacaaggtaa
     2641 ttatcattac acttggacag gaaattggaa tccaagtcaa ttaacgcaaa acaacacata
     2701 ctactacaac aactacaata cttatagtta taacaatgca tcttacaata actactataa
     2761 tcattcatat caatacaata actatacaaa caatagtcaa acagcaacaa ataactatta
     2821 tactggtggt tcaggtgcaa gttatagcac aacaagtaat aatgttcatg tgactacaac
     2881 tgcagcgcca tcttcaaatg gtcgttcaat ttctaatggt tatgcatcag gaagtaactt
     2941 atatacttca ggacaatgta cttattatgt atttgatcgt gttggtggga aaattggttc
     3001 aacatggggt aacgcaagta attgggctaa cgcagctgca tcatctggct atacagtgaa
     3061 caatacacca aaagttggtg ctatcatgca aacaacacaa ggctattacg gtcatgttgc
     3121 ttacgttgaa ggcgttaaca gcaacggttc tgttcgtgtt tcagaaatga actatggaca
     3181 tggtgctggt gtggttacgt ctcgtacaat ttcagcaaac caagcaggtt catataattt
     3241 cattcattaa tcaaatgtaa atcaaatgac gtcaatattc tctaacatga gagtattggc
     3301 gtttttgttt tatataaata taaatgagag cggtttattc actgatcttt agggaactaa
     3361 gtaataaagt gataatttat actatgtcag tatgattgag agtgattcaa tttagatgaa
     3421 aaccatgaaa aaatatatta aaacagcatt tttttgtagt atgtattggt taattgttca
     3481 actaaatata gcaaatttag gtacaagaat tcctgataag tattttcgtc agaagtacat
     3541 aatatttaaa tcatttaact ttgagaagca tggaaaattt tggaacaaat ggttttacgt
     3601 aagaaaatgg aaacataaga ttttagatgg tcatcagctt aatcaaaata tatatgatca
     3661 gcgtcattta atgacaatca atactgatga aattgaaaaa atgattatag agacaaagag
     3721 ggcagagttg attcattgga tatcgatact tccagtcatc atattcaata aaggccctcg
     3781 tttagtaaag tatataaata ttttctatgc aatgatagct aatgttccaa tcattattgt
     3841 gcaacgctat aatcgaccga gattaacgca gttactacgc atattaaaac gaagaggtga
     3901 acgtcatgac taaacatatc atcgttattg gtggtggctt aggtgggatt tctgcagcaa
     3961 ttcgaatggc acaaagtggc tattcggtct cattatatga acaaaataat catataggag
     4021 gcaaagtgaa tcgtcatgaa tcagatggct ttggctttga tttaggtcca tctattttaa
     4081 cgatgcctta tatttttgaa aaattattcg aatatagcaa gaagcaaatg tcagactacg
     4141 ttacaatcaa gcgattgcca catcaatggc gtagcttttt tccagatgga acgactatcg
     4201 atttgtatga aggtattaaa gaaacaggtc agcataatgc gatattgtcg aaacaggata
     4261 tagaggaact gcaaaattat ttgaattata caagacgaat cgatcgtatt actgaaaaag
     4321 ggtatttcaa ctatggttta gatacactat ctcaaattat taaatttcat gggccattaa
     4381 atgctcttat taattatgat tatgtacata ctatgcaaca ggccatagac aagcgtatct
     4441 cgaatccata cttgcgacaa atgttaggct attttatcaa atatgtaggt tcttcatcat
     4501 acgatgcgcc agctgtatta tctatgttat tccatatgca acaagagcaa ggcctttggt
     4561 atgtagaagg tggaatccat catttagcca atgccttgga aaagctagcg cgtgaagaag
     4621 gtgtcacaat tcatacaggt gcacgtgtgg acaatattaa aacatatcaa agacgtgtga
     4681 cgggtgtcag attagataca ggtgagtttg taaaggcaga ttatattatt tcaaatatgg
     4741 aagtcatacc tacttataaa tatttaattc accttgatac tcaacgatta aacaaattag
     4801 agagggaatt tgagccggca agctcaggat atgtgatgca tttaggtgtt gcttgccaat
     4861 acccgcaatt agcacatcat aatttctttt ttacggaaaa tgcttatctc aattatcaac
     4921 aagtttttca tgaaaaggta ttgccagatg atccgaccat ttatctagta aatacgaata
     4981 aaactgatca cacacaagcg ccagtaggtt atgaaaatat caaagtctta ccacatattc
     5041 catatattca agatcagcct tttaccactg aagattatgc gaagtttagg gataaaattt
     5101 tggataaatt agaaaaaatg ggacttactg atttaagaaa acacattatt tatgaagatg
     5161 tttggacacc ggaggatatt gaaaaaaatt atcgttctaa tcgtggtgca atatatggtg
     5221 ttgtagcaga taaaaagaaa aacaaaggat ttaaatttcc taaagaaagt cagtattttg
     5281 aaaacttgta ctttgtaggt ggatcagtaa atcctggtgg tggcatgcca atggttacat
     5341 taagtgggca acaagtcgca gacaaaataa acgcgcgaga agcgaagaat aggaagtgat
     5401 atctatgaaa tggttatcac gaatattaac agtaatagtg accatgtcta tggcgtgtgg
     5461 tgcattgata tttaatcgta gacatcagct aaaggcgaaa acgctgaact tcaatcataa
     5521 agcattaaca attattattc cggctagaaa cgaagaaaaa agaataggtc atttactaca
     5581 ttcgataata caacagcaag ttccagtaga tgtcattgtt atgaatgacg gatcgacaga
     5641 tgaaacagca cgtgtagcac gttcatatgg tgcgactgta gtagatgttg ttgatgatac
     5701 tgacggcaaa tggtatggga aatcacatgc ttgttatcaa ggtgtgacgc atgcatgtac
     5761 gaatcgcatt gcctttgtag atgctgatgt aactttctta aggaaagatg ctgttgaaac
     5821 gttgattaat cagtatcaat tacaaggtga aaaaggattg ttaagcgtac agccttatca
     5881 tataacaaag cgtttctacg aagggttttc agcgatattt aatttaatga cagtcgttgg
     5941 tatgaatgta ttttctacct tagacgacgg tcggactaac cagcatgcat ttggaccggt
     6001 gacattaaca aataaagaag attattatgc aactggaggt cataaaagtg caaaccgtca
     6061 tattattgaa ggatttgctt taggaagtgc atatacttca caatcattgc ccgtaacagt
     6121 ttatgaaggg tttccatttg ttgcatttcg catgtatcaa gaaggatttc agtcattaca
     6181 agaaggatgg acaaagcatt tgtcaactgg ggcaggtggc acaaagccta agatcatgac
     6241 agcaattgtg ttgtggttgt ttggttctat agcgagtatt ttagggctat gtcttagttt
     6301 aaaatatcgc caaatgtctg taagaaaaat ggtagcactt tacttgagct atactacaca
     6361 atttatttat ctgcatcgaa gggtcggcca attttctaat ttattaatgg tatgtcatcc
     6421 attgttattt atgtttttta ctaaaatttt catccaatct tggaaacaaa cgcatcgtta
     6481 tggtgtagtt gaatggaaag gtcgtcaata ttctatatct aaagaacaat aaatcaaggt
     6541 aatggcattt caatatagga ggactagtat gacaatgatg gatatgaatt ttaaatattg
     6601 tcataaaatc atgaagaaac attcaaaaag cttttcttac gcttttgact tgttaccaga
     6661 agatcaaaga aaagcggttt gggcaattta tgctgtgtgt cgtaaaattg atgacagtat
     6721 agatgtttat ggcgatattc aatttttaaa tcaaataaaa gaagatatac aatctattga
     6781 aaaataccca tatgaacatc atcactttca aagtgatcgt agaatcatga tggcgcttca
     6841 gcatgttgca caacataaaa atatcgcctt tcaatctttt tataatctca ttgatactgt
     6901 atataaagat caacatttta caatgtttga aacggacgct gaattattcg gatattgtta
     6961 tggtgttgct ggtacagtag gtgaagtatt gacgccgatt ttaagtgatc atgaaacaca
     7021 tcagacatac gatgtcgcaa gaagacttgg tgaatcgttg caattgatta atatattaag
     7081 agatgtcggt gaagattttg acaatgaacg gatatatttt agtaagcaac gattaaagca
     7141 atatgaagtt gatattgctg aagtgtacca aaatggtgtt aataatcatt atattgactt
     7201 atgggaatat tatgcagcta tcgcagaaaa agattttcaa gatgttatgg atcaaatcaa
     7261 agtatttagt attgaagcac aaccaatcat agaattagca gcacgtatat atattgaaat
     7321 actggacgaa gtgagacagg ctaactatac attacatgaa cgtgtttttg tggataagag
     7381 gaaaaaggca aagttgtttc atgaaataaa tagtaaatat catagaatat aggtggttga
     7441 ataatgaaga ttgcagtaat tggtgcaggt gtcacaggat tagcagcggc agcccgtatt
     7501 gcttctcaag gtcatgaagt gacgatattt gaaaaaaata ataatgtagg cgggcgtatg
     7561 aatcaattaa agaaagacgg ctttacattt gatatgggtc ccacaattgt catgatgcca
     7621 gatgtttata aagatgtttt tacagcgtgt ggtaaaaatt atgaagatta tattgaattg
     7681 agacaattac gttatattta cgatgtgtat tttgaccacg atgatcgtat aacggtgcct
     7741 acagatttag ctgaattaca gcaaatgcta gaaagtatag aacctggttc aacgcatggt
     7801 tttatgtcct ttttaacgga tgtttataaa aaatatgaaa ttgcacgtcg ctatttctta
     7861 gaaagaacgt atcgcaaacc gagtgacttt tataatatga cgtcacttgt gcaaggtgct
     7921 aagttaaaaa cgttaaatca tgcagatcag ctaattgaac attatattga taacgaaaag
     7981 atacaaaagc ttttagcgtt tcaaacgtta tacataggaa ttgatccaaa acgaggcccg
     8041 tcactatatt caattattcc tatgattgaa atgatgtttg gtgtgcattt tattaaaggc
     8101 ggtatgtatg gcatggctca agggctagcg caattaaata aagacttagg cgttaatatt
     8161 gaactaaatg ctgaaattga gcaaattatt attgatccta aattcaaacg ggccgatgcg
     8221 ataaaagtga atggtgacat aagaaaattt gataaaattt tatgtacggc tgatttccct
     8281 agtgttgcgg aatcattaat gccagatttt gcacctatta aaaagtatcc accacataaa
     8341 attgcagact tagattactc ttgttcagca tttttaatgt atatcggtat agatattgat
     8401 gtgacagatc aagtgagact tcataatgtt attttttcag atgactttag aggcaatatt
     8461 gaagaaatat ttgagggacg tttatcatat gatccttcta tttatgtgta tgtaccagcg
     8521 gtcgctgata aatcacttgc gccagaaggc aaaactggta tttatgtgct aatgccgacg
     8581 ccggaactta aaacaggtag cggaatcgat tggtcagatg aagctttgac gcaacaaata
     8641 aaggaaatta tttatcgtaa attagcaacg attgaagtat ttgaagatat aaaatcgcat
     8701 attgtttcag aaacaatctt tacgccaaat gattttgagc aaacgtatca tgcgaaattt
     8761 ggttcggcat tcggtttaat gccaacttta gcgcaaagta attattatcg tccacaaaat
     8821 gtatcgcgag attataaaga tttatatttt gcaggtgcaa gtacgcatcc aggtgcaggc
     8881 gttcctattg tcttaacgag tgcgaaaata actgtagatg aaatgattaa agatattgag
     8941 cggggcgtat aagggagtag tctaagagaa agatgtgaga aaagtataag gggaacgtaa
     9001 aaggcatagt gaaataagta tgtgcttaag aagttttatt taacaatgaa tgacgcctgt
     9061 taaggttcac gtttgaatac gtaccttgat gggcgttata ctttatataa gcatatctaa
     9121 ctggttgcgc atgcggatat gcatgacagt taaatcataa cgattgagtg catattaatc
     9181 tgaaaattcc taatattgac aataatatta tacctaccta aaatgaaaat aaattcattc
     9241 aatacaacgt gtgtcataag ttctgttgaa cataacggtg aatggcgatg tcattgtaac
     9301 catctaaatg agagtgatac aagttgttag gattatgata gtcgcccagg ctatgtttaa
     9361 tgatagagga ggaatttgat gatctctaac aaattagcaa atatccctga tagttatttc
     9421 gggaaaacga tgggacgtaa aatcgaacat ggaccattgc cattaattaa tatggcagtt
     9481 ggaatccctg atgggcccac gccacaaggt attattgatc attttcaaaa agcactaact
     9541 attcctgaaa atcaaaaata tggtgctttt catggtaaag aagcgttcaa gcaagctatt
     9601 gtagattttt atcaaagaca atataatgtg acgttagata aagaagatga agtatgcatt
     9661 ttatatggta caaaaaatgg attagtggca gtaccaacat gtgttatcaa tccaggagac
     9721 tatgtattac tacctgatcc aggctacacg gattatttag caggtgtact tttagctgat
     9781 ggcaagccag tcccgcttaa tttagaaccg ccacattatt tgccagattg gtccaaagtt
     9841 gattcacaaa taatagataa aacaaaactc atttatttaa cgtatccaaa taatccaact
     9901 ggatcgacag ctacgaaaga agtttttgat gaagcgatag ctaaatttaa aggtacagac
     9961 actaaaatcg tgcatgattt tgcttatggt gctttcggtt tcgacgctaa aaatcctagt
    10021 atacttgctt cggaaaatgg gaaagatgtt gcgattgaaa tttattcctt gtctaaaggt
    10081 tataacatgt caggttttag agtcggtttt gcagttggta ataaagatat gattcaagct
    10141 ttgaaaaagt accaaacgca cacaaatgct gggatgtttg gtgcattaca agatgccgca
    10201 atatatgctt tgaatcatta tgatgatttt ttagaagaac aaagcaatgt atttaaaaca
    10261 cgtcgtgacc gattcgaagc catgcttgca aaagctgatt tgccatttgt tcatgctaaa
    10321 ggtggcatat atgtatggtt ggaaacaccg cctggttatg acagcgagca atttgagcaa
    10381 tttttagtgc aagagaagtc aatacttgtt gctccaggga aaccatttgg agagaatggt
    10441 aatcgttatg tgagaatttc attggcgtta gatgaccaga aattagacga agctgcaata
    10501 agattaacag aactagcata tttatatgaa taataaagtt aagggtgatg ttaaaatgac
    10561 aaaaattaaa atcatgagtg tacgtgatga agatatgcct tatatcaagg cgtgggcaga
    10621 gaaacatcat gttgaggtag atattactaa agaagcatta actgatgaca atgttgaagg
    10681 tgtcgctggt tatgatggct tatcgctatc acaacaaata ccattatcag agcatgttta
    10741 taaacgatta aatgaactag gtattaaaca aatcgcacaa cgtagtgctg gatttgatac
    10801 atatgatttg gagttagcaa acaagtataa ctttattgtg tcaaatgtgc cgtcatactc
    10861 tccgaattca attgcggagt ttgctgtgaa ccaggctatt aatgtggttc gtcattttaa
    10921 tcaaattcaa acgaaagtgc gggagcatga ttttagatgg gaacctacta ttttatcgaa
    10981 atccattaaa gatttaaaag tagctgttat tggtacaggt cgaattggcc gtgtagtagc
    11041 cgatatattt gccaatggtt atcaaagtga tgtggtcgca tacgacccgt ttcctaatgc
    11101 taaaattgca acgtatgtcg attacaaaga tacgattgag gaagcggttg aaggtgctga
    11161 tatcgtgaca ttacatgtac ctgcaacgaa atataatcat tatctattta atgctgaatt
    11221 atttaaacat tttaaaaagg gcgcagtatt tgtcaattgt gcgagaggtt ctttagtaga
    11281 tactaaggcg ttattagacg cattagacaa tggtgtgatt aaaggtgcag cacttgatac
    11341 gtatgaattt gaacgcaaac ttttcccaag tgatcaaagg ggcaaaacac tgaacgatcc
    11401 attgttagaa tcgttgattg acagggaaga tgtcatatta acaccacata ttgcgtttta
    11461 tactgaagct gcagttaaaa atctaattgt cgatgcatta gatgcaacat tagatgtatt
    11521 gcagactgga gatactaggt tacgagtaaa ttaaaaatcg aactgatgag ataatttgga
    11581 ttgttgggat tctcatcagt tcgatttttt aatttggtgt tgatgacgtt gaatattgcc
    11641 taattaaacg acatcgtaac cttgatcttc aattgcgtct ttcatttgag atacagcaac
    11701 tttactgtca tcatattgaa cacttacttg accattttca aggttaacgt cagctgaagt
    11761 gacaccgtca atattattta atgcagattc aacagcactt ttgcagtgac cacagctcat
    11821 accttcaaca tttaaaattt cttgtgacat agcattcacc tccttttaga gccacttatt
    11881 atttataata attagtggct
//
LOCUS       DAKTGS010000089        11870 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.089, whole genome shotgun sequence.
ACCESSION   DAKTGS010000089 DAKTGS010000000
VERSION     DAKTGS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.089"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(263..1621)
                     /locus_tag="O0286_001900"
     CDS             complement(263..1621)
                     /locus_tag="O0286_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCY0170273.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(1738..3291)
                     /gene="gntK"
                     /locus_tag="O0286_001901"
     CDS             complement(1738..3291)
                     /gene="gntK"
                     /locus_tag="O0286_001901"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCY0170274.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(3315..3995)
                     /locus_tag="O0286_001902"
     CDS             complement(3315..3995)
                     /locus_tag="O0286_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0170275.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(4149..4913)
                     /locus_tag="O0286_001903"
     CDS             complement(4149..4913)
                     /locus_tag="O0286_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0170276.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(5078..5770)
                     /locus_tag="O0286_001904"
     CDS             complement(5078..5770)
                     /locus_tag="O0286_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0170277.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(6001..6417)
                     /locus_tag="O0286_001905"
     CDS             complement(6001..6417)
                     /locus_tag="O0286_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCY0170278.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(6556..8379)
                     /locus_tag="O0286_001906"
     CDS             complement(6556..8379)
                     /locus_tag="O0286_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCY0170279.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(8857..10134)
                     /locus_tag="O0286_001907"
     CDS             complement(8857..10134)
                     /locus_tag="O0286_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170280.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            10681..11292
                     /locus_tag="O0286_001908"
     CDS             10681..11292
                     /locus_tag="O0286_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCY0170281.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(11301..11399)
                     /locus_tag="O0286_001909"
     CDS             complement(11301..11399)
                     /locus_tag="O0286_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170282.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(11389..>11870)
                     /locus_tag="O0286_001910"
     CDS             complement(11389..>11870)
                     /locus_tag="O0286_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170283.1"
                     /translation="TLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMI
                     LRVTITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGK
                     VLGVFNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
BASE COUNT         3780 a         2281 c         1709 g         4100 t
ORIGIN      
        1 ggcttgtatt ttttatactt atttttttaa attaaattca tcattatcta atttaaaaca
       61 atatactaaa cgtttcataa ttatcgcctg tacaatacgc acaaaaacat gtcttgaaac
      121 gcctttcatc actctaaaat acccaatata ctttttatat cgttcggatt ctgagtattt
      181 cagacgattt tctgcataaa aataaacgtg tttcaaggca atatattgca attacctaaa
      241 aacacgttta cttaatattt agttaaacaa ataagctaat gaataaaatg aagatgatac
      301 ctgaaacgga aataatcgtt tctaataatg accatgttaa gaatgtttct tttacagtta
      361 aaccaaaata ttctttaaac atccaaaatc ctgcgtcatt tacatgagac aaaatcacac
      421 tacctgcacc tatcgcaagt acaactaatg caacatttac atctgatgat tgtaataatg
      481 gtaagacaat acctgtagtt gaaatcgcag ctactgtagc cgaacctaat gcgatacgta
      541 gcacagctgc aacaatccat gctagtaaaa tcggagacat ctctgtacct tcaaacattt
      601 tagcaattgt atttccgaca ccgccgtcaa ttaatacttg tttaaatgta ccgccaccgc
      661 caataatcaa taacatcatt ccgattggat aaatcgcatt cgtcactgat tccataatat
      721 gattcatctt acgctttctc attaatccca tcgtaacgat tgcaaataat actgctatta
      781 gcatggctgt ccctgctgtt cctatcatat aaatgataga ttcaaataga tttgtaggtt
      841 tgtcatgccc agttacaagt tgcgttatcg tagacactaa cattaatatg actggtaatg
      901 ttgctgttaa taaactcata ccaaatcctg gcatctcttg atccgtaaat tctttttgtg
      961 cacctaacgc tgaaatatcg ccttctcgtg tatacgcaga cggaatcatt ttttgtgcaa
     1021 ctttgttaaa tataggccct gcaatgagtg taactggaat ggcaataatc ataccataca
     1081 gtaatacatc tccaacattt gcctttaatt cttttgcgat gactaccggt cctggatgtg
     1141 gtggtaaaaa gccatgtgtc actgataaag ctgttaccat aggtagtcct agttttaaca
     1201 ctgaaacatt tgcgcgtttt gctactgtaa atactaatgg aatcagtaag actaaaccta
     1261 cttcaaagaa caatgcaata ccgacgataa atgctgcaac aagcattgcc cattgtacat
     1321 gtttttgacc aaatttttga atcaacgtgt ctgcgattcg agttgcacca ccaccatcag
     1381 caagcaattt cccaagtatg gcacctaaac cgaatatcag tgcaatgtgg ccgagcgtac
     1441 tgcccattcc tttctcaatc gtctccataa ttttagtcaa tggtatacct agcattaacg
     1501 ctgtaatcat cgatgtgata attaatgaaa taaatgtatt taatttaaac ccaataatta
     1561 atactaataa aataacgata cctaaaacaa cactgattaa cggccatatt tcgttaaaca
     1621 tgacattccc ctctttctct tttcaataga atgtaacacc gtcgtcgcgc acgtattcca
     1681 cataatatat agtagtcatg gcacaacaac tttatctaag atgcttgagt gacgtattta
     1741 ttgtgtttta ttttcagcga tatgttggcg ttgaaaatct gcaatttgtt cataattctc
     1801 tgttaaagaa cgacttaaat tgataaaaat ggatacgatc tcttggtaaa cagtgacatt
     1861 ttcttcaatc ggcgtatgat tgtttgtggc accgaccatc gatgaaacga ttgaaaaatc
     1921 ttcaatgtca cctacagctt taagtccgag cacgcaggca cctaagcatg aactttcata
     1981 actttcagga accactaact ctgtgtcaaa tatatctgac atcatttgac gccatacttc
     2041 acttttcgca aaaccacctg ttgcttttat catcttaggt gtttcattca ttacttcaat
     2101 aagcgcaaga tagacggtat acaaattgta aagaacacct tctaatgcag cgcgaatcat
     2161 atgttctttt ttatgagata aagttaaacc gaagaatgaa cctcttgcat ttgcgttcca
     2221 aagcggcgca cgttctcctg ctaaataggg atggaatatt aaaccatctg cacctggttt
     2281 aacacgcttt gcaatttgag ttaagacatc ataaggatca acaccgagac gtttcgcagt
     2341 ttcgacttca ctcgctagca actcgtcgcg caaccatctc aatacgacac caccattatt
     2401 tacaggacct ccgatgacgt agtggtcctc tgttaagaca taacaaaata ttctaccttt
     2461 gtaatcagta cgcggtttat ctatcacagt acgaatcgcc ccagatgtac cgattgtgac
     2521 agcaacttct cctttaccaa cactattgac acctaaatta gaaaggaccc catcactcgc
     2581 accaataaca aacggtgtat ctttattaag ccccattaat gttgcataac gttctttcat
     2641 acctttcatc acatacgttg ttggaactaa ttccggcaac atttccttgg aaatacccag
     2701 cagttctaat gcctcaacat cccaatctaa tgtttctaaa ttaaacatcc ctgttgcgga
     2761 agccattgaa taatcaatga tatatgtatc aaataaatga tagaaaatgt atgttttaat
     2821 atctgcaaac ttagcagtac gttgaaatac atcttgccat tcatgtttca tccaaaaaat
     2881 cttcgctaat ggcgacatag gatgaatcgg tgtgcctgtt cgctggtaaa tcgcattgcc
     2941 atcatgcact tcatttatta ctgttgcata ttttgcagcg cggttatctg cccaagtaat
     3001 attatttgtt aatctttgat gttgctgatc catcgcaatc aagctatgca tttgcgcact
     3061 aaatgacaca aacttaatgt cgtctttatt aactttggat tctctcataa catatttaat
     3121 agtcattagt actgcatcaa ataattcatc tgggttttct tctgagacat caacgtttgg
     3181 tgtgtgtaaa tcatagccta tttgatgttt catgataaaa gttccatttt catcatataa
     3241 gactgacttg gtactcgtcg ttccaatgtc gacaccaatc atatatttca tgataaatcc
     3301 ttctttcttt cattttaatt caaccaaaat ccttcaatat ctttaccaac atcgtcgaaa
     3361 tttaaatgaa acgcttcttt caaaatttga ctgtcgtatt gttccactgc atcaataaac
     3421 acttgatgat tatgatgtat gcgttcaaaa tcttgcgggt tctgttgcat acgttgtcgc
     3481 atggatgtta aaacgagtgc catcattact ggttttaaat gactccaaaa tgagtttaaa
     3541 tattgatgat tagatgcttt gattaatgtt tcatgaaatt caaagtcatg cttcgtaaat
     3601 gattctgcat cctcaaattt tactgccact ttcatcattt caagttgttt cttcatttct
     3661 tttacgatgg gtagtcgctc ttgattttta actcttgaaa atgcaaatga ctctaacatc
     3721 agtcgcaaat catacatttc tttcttttct tgttccccaa acggcaacac atgtgcaccc
     3781 attctttcta attggatgag ttgattttgt tgcaataatt taaatgcatc tcgaattggc
     3841 gaacgactca cattaaattg ctttgccatt tgattttcag tgagtaacgt accttcagct
     3901 atgtgaccat tcacaatgcc taagcgtaat tctgccgcga taccttctcc agttgtcata
     3961 ccttccaacc atttctctgg atatccatac atcatcaaag tcactccttc attacacgac
     4021 atacttgtat acaagtatgt taatatagtt attatgagtt tgcaagcgct ttctttacga
     4081 gcactaaaat agtgaccacc ccttttcgat ttaaatttaa aggaaatggt cactatcaca
     4141 cgaatgattt aattgttatg ttgtatgtgg gatatttcta attgttctgt actcatatgc
     4201 gctttaggta cttcaatgca ataatgcgtt tcatgacagt ttggacattc gaatcgacgt
     4261 gttgtcgctg tatgtttcgc tttgataact gcccacaaag atggtgagaa tatatgctgg
     4321 cagttaggac ataaataggc aaccttttgt tggtaataaa aagtaacacc aatgccataa
     4381 ccaatcataa atggtaaagc aattaaaaac ggccatttat ttttcatcaa aattgcactt
     4441 ataatgctag aatattgaat tattcctata ataccagcac taatccaaat gttacgacga
     4501 atacttttca tttcagctga tttactcatg acatgctcta tgtcttttaa gtgtgtgatt
     4561 ggagacgtcg acgcttcatt tacgtaatat tgaacatttt taattttgtt taataccgct
     4621 tgttgctgtt taacttgttg gttaatttct tgttgtttca tagttagtaa agtattgagc
     4681 gtcttcaaag taccttcacc ttttagcaac atatctatat cgcttaacgc acaacctaaa
     4741 tctttaagca ataagattaa ctctaatgtt tgtcgctgtt gttctgtata cacacgacgc
     4801 tttccttctg taaatccttg tggtttcaaa atacctttgc gatcataata ttgaatcgtt
     4861 cgtgttgtca cattgcataa ttttgcgagt tctccagtcg aatagttaga catagattcc
     4921 acctcctata attaccatag ttgatgaccc gacgtcacga gcaagtacaa tttccacatt
     4981 ttaaagaaat ttattatact aggcgtctta tttttatgat ttcgtaccat gttgatttac
     5041 aaactcactc aaactaagta acacacctac taaacatcta ctctgttatt tcagaatgaa
     5101 tttgttgtaa tttatcttca acttcagtaa tctctgtcgc acattctttc agtaaatctc
     5161 gatacttttc cgtctctgca ttgtttttat aacgtatttt atgttctaaa cttgcccaca
     5221 tatccatacc tatcgttcta atttgaattt caacaggcaa tacctcaacg cgttctgcta
     5281 aaaagactgg aatagataca acgatatgta aactgcggta accattttct ttagggtgct
     5341 gaatataatc tttacgtttt atcaattgta cgtcttcttg tttaagcaac atctcttcaa
     5401 tcaaataaat atcatctaag taattacata ctacgcgaat tccggcaata tccagtatat
     5461 gttctttggc agtttctgcg ctaatctgta atcctttacg attaagcttt tctattaaac
     5521 tacgcatttc tttcacacgt cgctccatat gatgtatagg attgtgttta taaatgtgat
     5581 tgaaattgtc atctaaaata ctcagtttag tgcttatttc ctttagtgct gacgaataaa
     5641 tatgatctaa ctctacgaaa cctaataacg tatcaaatgc ttcatcacca ttttcaaatt
     5701 tacttaagct atttttaaaa tcatgtcgca aatcctctaa atatagtgat ggttttcgat
     5761 ctacatacat ttttatacta acctccgata tataatcatc tttattgtac ctaacatttt
     5821 attaagataa tactaataca ctatcgaact ttgggctgat actgaaacag cacaaagaaa
     5881 ctcaaacaat tcgaattatg tatcaaaacc ttcaattaca ggaagtgatt ttaaaatgat
     5941 ttaacgcaaa aaaacacctg ttaccgttat ataggtacaa gtgcttaatt gatagagatg
     6001 ttatacgtct ttaaatgatt ccacaacttt tggatgtgga ccatccataa gcggttctct
     6061 ttgtcgaacg ccaccacttt ggttgccaat tgattcgcta tcaaagtacg atttatcttg
     6121 atttgattgt tcttgaatat gttcttcatt atcagttgtt gcatattcac tatccacctc
     6181 tgttttttcc attgttgctg tatggaattg tacgaagttc tcttcttcta atgctttgat
     6241 ttcttctttc gataacgctc gataccaatc tttcgctttt ttcgccgcaa taggaacaac
     6301 atctttttca agatatcctt cttttaacat tgcattcgca atttctatcg ctttttgccg
     6361 tttaattact tcaaattctt tccatttttc ggggtaacct ttcattgtaa agggcatttc
     6421 cttaacctcc aatatgttat aattcatgtt atataccacc ataattataa aatgaaacat
     6481 attaaacaca tttttttaga ctcccgtcat aataacttca gacaaacgtt agtctgacct
     6541 gataaatatt taattttaaa cgttcttgtc tctctcacga aaaccattgt gttgaatatt
     6601 catcattgct gattgaatat aattatactg tggtaaatct ggtaatatct ttatttctaa
     6661 ttctgtattt aattcgaaat gcttagcgat gttttcaaat attgctaaat actcgcccat
     6721 taattgttca tttattgtaa gtctatcttc attagccatg gctctattta acataaagct
     6781 aatttcttct aatgcgaata agctaggata ataattttga atcaatgtct tatcactaaa
     6841 taattcacca ttagctgcat tataaacttg cgtcatgtta tttaatttca cagataggtt
     6901 catagccgtg tttttctgat acacatcatc tttatattga ttttcagaaa ataaataatg
     6961 aaagagcgtt gcttctttac gaacaacttc agccattaca ttaggaagca atgcggatgc
     7021 ggtacgttgt ccaaggacaa ataaacctat gattgcaatc acaataccca ttgcaacgtc
     7081 aataactctt ggaaacgcaa tgttaattgt taaattctgt gatgctaatc cgttcattaa
     7141 aataacttgt attgtaataa aaactactgc aatcgcataa tttgctccca ccaatgcttc
     7201 agtaaacaat gctgcaatgc ccattaaaat tactgcaaca ggtgttggta ttgaaaacaa
     7261 caatatgact gaaagtacta acacacctaa aatagtacct aaacctcgtg ccatacctct
     7321 ctcgattgca tgtatagttg atgttcctag taatattgta tgtgcagata acggtatcca
     7381 gtatgctttt tcaaaatcaa acattaacgc aataaatatc gctatcatca taatcgctgt
     7441 atatctcaat gtatttctaa aaacaataga gtctaatgtt aaattttgat aaatgcgttt
     7501 actatataaa ggttttcgca tgtctgcttc atacgcaata cgcgcttcat ttgcatgcac
     7561 catttcatca attttcaata tatggttgaa taaattttga aattcctctg ttactgtcac
     7621 ttcttttcgc caatatctta cattttcctc actattatct agttgtgcaa tgatatgatc
     7681 catcatttca attaactcat ctggcaatgg tcgaatttgt ttagcgttca actctaacag
     7741 ttcagaataa atcccttggg cagatgtgtg taataataat aatttttgga aacgtctact
     7801 taatttatcg ttacttgaag tagaagtgat taataattta tcagatgctt taaaactatc
     7861 caccgctgtt tttgctactt gtaaaaaagc agatttatca ttataagtgt gtaacaactt
     7921 agatatgagt ttaaaatcat tttgaattgc ttgttcttca gctttgtttt tagaaaatac
     7981 gatcgtgatt aacaccatca tagtggcaag tataccgcct actaaaatcg caaagcctct
     8041 atataatgct tcttcgggag ctataggtaa gttaatgggt agactgaatg tcacaatgaa
     8101 gaatgtcgat gacggtccag ctatttttaa ggcagtaaat atataaaatg gtataaccgt
     8161 aacaattaat aatgtcattc caaaaacgag tggcgttttg gctgttagcg taccaagcat
     8221 catacatatt gcaaacgcta aattacaaat tattacagtt cgcagcttag atcgcgacgg
     8281 acctttaaaa acataaatat gggctagtgt tccggttgca actaataatc caaattggaa
     8341 attaccacat aagtaaccga ttattgcagg aataatcatt aacagtccct gtcttacacc
     8401 tttataaaag tctaagttaa gtcgattgat ttttatgagt gatgtcaaaa agatgtcatt
     8461 tcgacttctc ctttccttgt caaaatattt ccataatctt tttattagca tagttaaaat
     8521 gaatttgttg ctataaattt taattacgca acatatgtca ttataacacc ttatgctcaa
     8581 ttatctaaat ttaaaaactt ttaaagtcca attaaaatta atttctaatg ttatttaaca
     8641 tcgttgaacc tttggtcgcc cacctttgcg ctagttaaaa tttatatgta cttttacaag
     8701 cgttacctga ctaatgtttt aaaaccttat ttctttacat atatcatttc aataacttca
     8761 ttttcatctt aaaccatatc tgttttaaaa agtttcaatt tcaacgcaaa aaaagcaata
     8821 agccccaact atgtgaaagc ttactgcttg tttaaattat ttttctattg ctttatcatc
     8881 actaacaatt aaaggttgat ctggtttcac atagaaccac ataatcacag cgattactgc
     8941 tggaacgatt aacaattgga atgtcattgt ccaacccaaa tgttctacga atagacctgc
     9001 taatagcgga ctgattaatg cacctatatt accccataag ttcatccacc ctgatactgt
     9061 accagagaag ttacgtccta agtcagttgc tgcagcccaa ctcataccca ttgaaatacc
     9121 gataccacca agacctagtg ataaccagaa aatacttaca tataaatttc ctgtgcgtac
     9181 agcaaagata attgaaactg caaacacaat aaatcctgca attgcaatta cacctctagc
     9241 tacgaatttt gaacgaccta atcctaatac acggtcagaa attgcacctg ctgataagat
     9301 taagaagaac attaataacc aaggtaatga actaatgctc atttctttaa agttaacgtg
     9361 gaatacttct gttaaatacg tcggtaacca aattaagaat aatgtaatga taaattgtac
     9421 aacaaagtat tgaattgcaa ttgcatagaa gctaaaacgt ttaaagaaat cattccatgg
     9481 tggtgatgac ttttcagtag ctacgatatc acgattttcc ataatgaaac gtttctccgc
     9541 ttcattaacc attctatgtt gctcaggtaa gtctttggca ataatcgccc ataatacagc
     9601 cattaaaata cctactgcac caaaaatgta aaatactgct tgccagttaa atgcgttaac
     9661 aatagcaatt gtaactattg gtgctaatac aggtccgaaa tatgatcctg ctaataatgc
     9721 acttgatgct ctaccttttt catttttaga gaaccaaaat gaattaaaca cagcattaga
     9781 agggtacatt ggcgcctcac ctacaccaaa taagaatctc actaaataaa ttaaaccgtg
     9841 gttcttaatc atacccgtta agattgtaaa tgcactccac caaactaatg caattgtaat
     9901 catcttacgt ggaccgaatt tttcagccaa catccccgat ggtacttgca ttaatgcata
     9961 acctagagag aagaatgatg ccaataaacc aaattgtggt tttgtcatcc ctaaatcatc
    10021 catcatttgc ttagcgatat atgaaatgtt tgctctatcc atatacgcaa taaccccaat
    10081 aaagaagaac gcaagcgcga acatccacct tacgttagtt cgtttttcct tcataactat
    10141 tcctacttcc ctaattaatt ttttaaaact taccctgaaa cgttaaaatt ttcggacatc
    10201 tggcatgtcc acccctactt tgtcacaaaa ttgtaccaaa gttatttttt ataatattat
    10261 cagacatgga aagcgtttac aatagaatct ataaaaattt tattaaattc cgcttaactt
    10321 ttgctattaa aacgatataa aggtactttt agttacatag tagcctatcg tttgttaatt
    10381 aaaaatactg caatcctagt gttttaaaac gtatattgca ggctttctta catattcgac
    10441 attcatttta aattgcatat aactacatta accattcatt gtgcagcgcc tgttgtttag
    10501 atgatcattt attgactttt tgctcttata gcaagcaatt ttttgattta ttgcttataa
    10561 tacattttaa gacaaatgtt tgtttcaaca tcgcatgcga catcattaat tattcatttg
    10621 aatttatcca gtttctattt gtctgcaatt gctgaaaatt aaaggagctt atttttaata
    10681 atggaacaaa ttatcactga atttattagc cgttttgggt atgcagccat ttttatatta
    10741 attttattag aaaatgtact acctatcgta ccatcggaaa ttattctgac atttgctggt
    10801 cttatgtctg ttaaatcaca tttatcaatt ttaactttat ttattattgc aactatcgca
    10861 tcgtttatag ggctgttaat tttatattat atctgccgtt tgatttcaga agaacgtcta
    10921 tatcgtttta ttgatcgaca cggtaagtgg attaaattga aaagtaagga tttaaagcga
    10981 gcaaatgatt ggtttaaaaa gtatggcgta tgggctgtat ttatctgtcg tttcatacct
    11041 gtattacgtg tattgattac cattccagct ggtgtaaacc gcatgaatgt tgtgacattt
    11101 accgttattt cattaatagg tactacaatc tggaatttcg gtttaatttt actaggacgc
    11161 actttgagcg atagctttgg tatgttgatg actggtcttc atacatattc acgtattatg
    11221 tatgttgtca tcattatcgc agtcatttac tttgctatac gttacattgg caaacgtaag
    11281 agagtaaaat aatattgttg ttagcataaa ggcatgaatt ttaaatttct ataaacaaaa
    11341 agcagtgaat cattccatct attgaaatga ttcactgctt ttttaagttt atttggcata
    11401 ctttagctga aagtcaacta tctttatata aagaaatatt actattaaca tgatagttat
    11461 agttgtgaag taaaaggcta ccaacggctg agaaatgata ccaattaata atgcaccgac
    11521 agtcattcca atatatctca caaaattaaa tacacctagc actttccctt ttatatgttc
    11581 aaacctttta gaaattatag tagacagcaa agctggcaca ataccaacac aagtacctaa
    11641 tataaatata attactgata atataattac atttgattcg tttaaccatc caaacaataa
    11701 cgtaaatata gcaaatccag ttatagtaac acgaagtatc attatttcat tatatttttt
    11761 tgaaaatcgc ttataaaata aacttcctaa tataacacta acagtaatag gtaaaaacaa
    11821 aactcctgat attccaacgc ctatatcata ggtattattt aataatgttg
//
LOCUS       DAKTGS010000090        11800 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.090, whole genome shotgun sequence.
ACCESSION   DAKTGS010000090 DAKTGS010000000
VERSION     DAKTGS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.090"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            66..194
                     /locus_tag="O0286_001911"
     CDS             66..194
                     /locus_tag="O0286_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170284.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(338..2266)
                     /gene="abc-f"
                     /locus_tag="O0286_001912"
     CDS             complement(338..2266)
                     /gene="abc-f"
                     /locus_tag="O0286_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCY0170285.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            2519..3154
                     /locus_tag="O0286_001913"
     CDS             2519..3154
                     /locus_tag="O0286_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCY0170286.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            3458..4537
                     /locus_tag="O0286_001914"
     CDS             3458..4537
                     /locus_tag="O0286_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCY0170287.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            4597..4821
                     /locus_tag="O0286_001915"
     CDS             4597..4821
                     /locus_tag="O0286_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCY0170288.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            5029..6279
                     /locus_tag="O0286_001916"
     CDS             5029..6279
                     /locus_tag="O0286_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCY0170289.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            6462..7412
                     /locus_tag="O0286_001917"
     CDS             6462..7412
                     /locus_tag="O0286_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0170290.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            7561..9045
                     /locus_tag="O0286_001918"
     CDS             7561..9045
                     /locus_tag="O0286_001918"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCY0170291.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            9042..10002
                     /locus_tag="O0286_001919"
                     /pseudo
     CDS             9042..10002
                     /locus_tag="O0286_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
     gene            complement(10190..10297)
                     /locus_tag="O0286_001920"
     CDS             complement(10190..10297)
                     /locus_tag="O0286_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170292.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(10369..11085)
                     /locus_tag="O0286_001921"
     CDS             complement(10369..11085)
                     /locus_tag="O0286_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0170293.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(11104..>11800)
                     /locus_tag="O0286_001922"
     CDS             complement(11104..>11800)
                     /locus_tag="O0286_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCY0170294.1"
                     /translation="ISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVN
                     ILTTLSEYIREDDMPGLRDYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKI
                     LRAQEMNFPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIES
                     ENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIE
                     NGFFIQKVEIINN"
BASE COUNT         3975 a         1674 c         2101 g         4050 t
ORIGIN      
        1 ataataataa aaatatactt caatattatt attatttcat aaagaaaatg tcttaacaat
       61 gaattttgat aaaaacaatg tcaatagaga acatgcgtac ttatcaacaa agagtgaata
      121 acttgtggat aaaaggtgtt gataacgtca tttattgtgg atatcaggtg ggaaaacaga
      181 aaataaatga ttgattagtg ttaatttact tgtgggtaac taggataagt ctgtggatag
      241 ctataatatc agcgttttta ttgtgaataa aaaaattaaa atttgtaaaa aaataactct
      301 aactatagcg attttacagt tagagttatg tatattttta tagtttttgt tgtaattctt
      361 cccatttaga catagcgtgt tctaattttt gttcgctatc ttgtttttgg atagctaatt
      421 cgtttgcttt ttgtggattg ttatacactt ctggttgggt taattgctcg tctatttgta
      481 agatagtagt ctctaaagtt tctatttcat tttcacactc ggatatttgt cgttcaagtt
      541 tgcgttgttc acgtctttgt tctttttggt tatgatacga cgattgttcc atagactttt
      601 tatgtgcttg tacgttaact gactgttctt cttggtgcgc tttcaacgct tccgcttcct
      661 caactttttc aatgtaatat tgataatctc ctaaatacat ctttccgcca tcaattgtta
      721 aatcaaatac cttgtttgct aattggttaa taaagtaacg atcatgggaa acaaatagta
      781 ttgttccttc aaaatgttgg agtgcttgct ctaacatttc ttttgaatct atatcgaggt
      841 ggttggtagg ttcatctaaa ataagtacat tgtcacgttg caacataagt agtgctagta
      901 gtaaacgtgc tttttcacca ccagataaat cattaattat ctttttaaca tcgtcttgta
      961 caaataagaa acgtccaaga actgctcgaa tatctttttc attcattaac ggatattgat
     1021 cccacacata atctaaaatc gttttactag atttaaattc tgcttgcttt tgatcataat
     1081 aaccaatttg taaatttgcg ccgaaagtaa tatcgccatt aagcgctttt tgttgattag
     1141 caatagtttt aattaaggtc gattttccaa taccatttgg cccaatgatt gctatatgat
     1201 cgcctttaga gacctctata ctcataggtt tggtaattgc agtttgataa ccgatttcta
     1261 aattttttac atgcatgacg tcattacctg tattccggtc aaagccaaat tgaatatttg
     1321 cacttttggc atctaacatt ggtttatcaa tgcgttccat tttttctaaa atcttacgtc
     1381 tactttttgc cattccactt gttgaagcac gggtaatatt tttctcaaca aaagtttcta
     1441 atcgttttat ttctgcttgt tgactttcat attcttgcat tcgtttttga taatataaat
     1501 cccgttgctg tataaattcc tcgtaattac caacatagcg tttgacatca cctaaagcca
     1561 catcataaat ttgagtaact attttatcta aaaagtaacg atcatggctg atgatgacga
     1621 ttgcaccttt aaaataacgt agataatctt caagccactt tgtcgtttct aaatctaagt
     1681 ggttagtagg ttcatctaaa agtaataaat caggttcgtt taatagcatt tgagctaaag
     1741 ataaacgtgt tttttggcca ccgctaaaat cattgatagg tttattgaaa tcttcttcac
     1801 taaaatttaa cccgtaaagt actgttttaa ttttactttc atattgatat ccttctaatt
     1861 gttcaaattg attcgataaa gattcataac gggacatatg tgttttatat gtatcactat
     1921 cgtaatcatt tgcatgtttt gataaacaat ctgtttcttc tttgattaag ctttccattc
     1981 gtttaatatg ttcaaatggt tttgacattt cttcaaaaac tgttgcatta gaatttaaag
     2041 tcatttgttg agttaaataa cctagtttta agtttttaat ttttgacaca tttccagaat
     2101 catagttttc tacgccagct ataattttca tcaatgttga tttaccggcg ccatttcttc
     2161 ctacgatacc aattcgttca ccagttttta cttcaaaatc aacatcagta aatatatctt
     2221 caccatcgaa cgattttgat atatgattga gttgtaaaag tatcattcga tcttcacctt
     2281 tcgtctttct caaattttat taacttatga aaaaagtcgt aattattaca ttaataacca
     2341 ttttacagta taaaacgccg tcttgaaact aagatattta tttttaaaat tcaataatgt
     2401 ataatattta ggtattcatt gtaatcaatt atttgttttt taataatcta acaattacgc
     2461 tctaactcgt ttgaatacat agatataatt cattcaaaca cgaaggagga aatagcgaat
     2521 gagtgaccaa gttaaaattc ctcgagcaac tttaaaacgt ttgccgttat attatagatt
     2581 tgtcagttca ttaaaatcta aaggtataga tcgtgtaaat tcaaaagcga ttagcgatgc
     2641 gttacaaatt gactcggcaa caattcgtcg tgacttttca tattttggcg aattaggtaa
     2701 aaaagggtac ggatataata tagatagttt attggatttc tttaaatctg aactaagcga
     2761 gagtgacatg atcaaaatcg caattgtcgg agttgggaac ctagggaaag ctttgctcac
     2821 atataacttt tcaatacatg acgatatgac gattacagaa gcgtttgacg taaaagaaga
     2881 tgttattggc cagaaaatag ggaacgttat tgttaaagat aacgatgaat taataacaac
     2941 attgaagaag gaagaaatag atgttgtgat tctaactaca ccagaaagag ttgcacagaa
     3001 agttgcagat gaactcgtcc aagctggtgt gaaaggtatt ttaaacttca ctcctggtag
     3061 aattaatacg ccttcagatg tgcaagtaca tcaaattgac ttaggtatag aattacagtc
     3121 attattattc tttatgaaaa attacagtga ataaatgtga tgttagcttt gaatgattaa
     3181 ccttgttata tctttaatta attatagaac agtctgggac taagtatgtc tcaggctttt
     3241 tctgttatgt ttgaaaatga aaggaaaaac tgttgtgttg ataaatatag tataggttaa
     3301 taaattaaca cgtagagata taatgatgaa tcgtaattaa tcgccttcct tatatgaata
     3361 taatataaat atcaaaaata ttgtgtataa ggttgaaaac aagaaaatca tcacctataa
     3421 tacatactaa acttatcgga aatgagaggt taagattatg tggatgattt ttagtggctt
     3481 aattgtcggg ggactcttag gttttgtcat gcaaagaaca agattttgtt taacaggtgg
     3541 ctttcgagat atgtatgtgc aaaagaataa taagatgttc tatgcattat taatcgctat
     3601 tactattcaa agtataggat tattgatttt gacggcaaca gatattttac aaattcctgc
     3661 acatagtttt ccaatattgg gaacaattat aggttctttt atttttggaa ttggaatagt
     3721 attggctgga ggatgtgcaa caggtacttg gtatcgcgct ggtgaagggc taattggtag
     3781 ttggattgca ttagtattat atgctgttac tgcagcaatc actaaaacag ggattttaaa
     3841 gccagtaatg gataaaatta atcaaccaac gaatgtaaat agtgatatgt ctcaaacaac
     3901 tggcattccg ttttggggat tagtcgttat attaactata atcaccattt ttctagttgt
     3961 aagaacactc aataataaga aagttcgagt tgctgttcca aaactaaagc aacgttatac
     4021 aggtattaga tattaccttt tcgaaaaacg ataccatcca tttattgcag caattgtaat
     4081 tggacttatc gcactcttag cttggccaat gagtgcatca actggaagaa atgacggttt
     4141 aggtataaca acgccttcag caaatttagt acactttttg attacaggtg aaactaaatt
     4201 tattgattgg ggtgtctttt tagttctagg aattttcatt ggttcatata ttgcagctag
     4261 aggatcaaga gaatttaaat ggcgattgcc agacaagatt acaatacgaa acagtgccat
     4321 tggtggcata tgtatgggat ttggtgcgtc agttgctggt ggttgttcta tcggtaacgg
     4381 tttggttgaa acggcaacga tgacttggca aggatggatt gcgctagcat cgatgatagt
     4441 tggtgtatgg acaatgagtc attttatctt tgttcgtcca atgaaaaaag tacaccaaca
     4501 atctgcaaag gttaaacagc aaacgcaaat agtatagaag attattatgc aaatgatgtt
     4561 gatcaaataa aagtgattgg aaaaggagaa ataattatga tacacgaatt aggtacagta
     4621 ggaatggtat gtccatttcc gttaattgaa gcgcaaaaga aaatggcaac attgcaatct
     4681 ggagatgaat taaaaattga ttttgattgc acgcaagcga cggaagccat tccaaattgg
     4741 gctgcagaaa atggttatcc tgtaacaaac tatgaacaaa ttgataatgc ttcatggaca
     4801 attacaattc aaaaagttta acgttatcat tttaacaata aaatagatat tagattctat
     4861 ggctacttcc gctaatttaa aagtgagtaa gtagtctttt ttgttttagt tcatgaaatc
     4921 atttttatat agtgtggcac attttattcc aaaagatgta ataaaactta acgcattttt
     4981 gctttttata aattgtcaga ttattatgaa aaaaagggag tggtaagtat gaatcttaac
     5041 gatacgatat ttatgttttt gtgtacatta ttagtttggt taatgacacc aggattaagt
     5101 ttattttatg gtgggttagt tcaatctaaa aatgcgctta atactgtcat gcaaagtatg
     5161 gcagcaattg tgcttgttac atttgtatgg ataacagttg gttttacaat tagttttggg
     5221 aatgggaatt tatggttcgg aaattgggaa tatacttttc ttaatcatgt aggttttgcg
     5281 actcaagaag atattagccc acatattcct ttcgctttgt ttatgttatt tcaaatgatg
     5341 ttttgtacga ttgcaatttc tattttatct ggttcaatcg ctgagaaaat gaagtttatt
     5401 ccttatttat tattcgtagt aatatggact gctcttgtat acagtccagt agcacattgg
     5461 gtttggggcg gcggttggat taacaaactc ggtgtattag atttcgctgg aggtacggtt
     5521 gttcatatta catcaggtgt ttctggttta gtattagcta ttatgattgg aaaaggaaac
     5581 aaacattctg aatcaacacc acataatctt atcattacgt tgattggcgg tatattcgtg
     5641 tggattggtt ggtatggatt taatgtaggt agtgctttta catttgataa tattgcgatg
     5701 cttgcattta caaatactgt catttcagcc agtgcaggtg ctataggttg gttaatttta
     5761 gaatatattt ttaaaaagac gacaagttta cttggacttt tactcggtgc attagcagga
     5821 ttagttgtca ttactcctgc agcaggatat gtaacatatc ttagtgcaac aataatggct
     5881 ttaataggag gtatctgttg ttatattgtc attaattaca tcaaggtaaa actaaaatat
     5941 catgatgcat tagatgcatt tggtattcat ggtgttggtg gtattattgg tgctgtttta
     6001 acagcagttt tccaaagtaa aaaagccaat cctgacattg agaatggctt tatttatact
     6061 ggtgacatac atattatact tgtacaaata ttatgtgtaa cagcagttgt aatttttagt
     6121 atagtcatga cgtttattat tgcgaaagta attaaattaa ttacaccatt atctgttacg
     6181 gaacaagaaa cgaatatagg attagacaag attgttcacg gtgaacatgc ttactttgaa
     6241 ggtgagctaa atagattcaa taaacatatt cgatattaga atatatttac atagaatatt
     6301 cattgtcctg acatttaact aaaggttgat gttgggacat tttgttatac aaaagtttta
     6361 ttttgaaatc tttttatgaa agaagcagaa atattattta aagcggttac acatatgcta
     6421 aaataaggct aagtgtcaca aataatgata ggtgaataag tatgaaaaat atatctgata
     6481 ttgccaaatt ggcaggcgtt tcaaaaagta cagtatctag atttttaaat aatggatctg
     6541 tcagtaaaaa aacaagtgaa aaattaacaa gaattatagc agaacatgac tatcaaccga
     6601 atcaatttgc tcaaagttta agagcgagac aaacacacct tattggtgcc attataccta
     6661 gaatgaattc atatgcagta gatgaaacaa tcaaaggatt ggcaaaacaa tgccaaaaat
     6721 atgaatcaca attaatttta aattacacag gtttaaatat cgaagcagaa atacaagcgc
     6781 ttgaaacatt agcacgcagt aaagtagatg gtattgtttt aatggctaca gacataacag
     6841 agagacatat tgaagtcatt aataaaatga atgtaccaat cgttattgtt ggtcaacaac
     6901 atgaacaact tcatagtatt gtgcatgatg attataaagc aggtcaaatt ataggcgaat
     6961 ggattggtca acagggatat caacaagttg aagtgtttag tgtaagtgaa aaagatattg
     7021 cagttggtat acatagaaaa cgtggtttac ttgaccagtt agctaaatac caaattaaac
     7081 ctaatataca tgaaacaaat tttacttatg tggaagcaca aaaagatgtt gcaaatgttt
     7141 tggaaaatgt ggagcaagta gatgcggttg ttggagcaac tgatacgatt gcattagctg
     7201 cctataaata ttattctgat aaaaaagatg ttatgaaacc acatcaaata tatggttttg
     7261 gtggtgaccc aatgacacaa ttagtgtctc catcgataaa aacaattcat tataattatt
     7321 ttgaagctgg ccaatgcgcg atggaagaga tacaacagat gcttaaaaag caagatatgc
     7381 catatagcgt cacagtagat gttaatattt agacgctgta ttttttaaaa taaatgtgga
     7441 accgatacca tataactata aatggatagg ttaaaagtta aagaacgtag gtaaaatttg
     7501 ctataataga atataaattg ttattagcat aaattataaa aggaggactg ggtaaatatt
     7561 atgaccgaat ggactagaga agaacgttat caacgaatcg aggacgttga tactgagtat
     7621 tttaaaacat taaaacaaca agttgatcaa tcaaaatttc gtcaacaatt tcatatacaa
     7681 ccagaaacag gcttattaaa tgaccccaac ggacttattt tttataaagg gaagtattat
     7741 gtttcacatc aatggttccc attaggcgca gtacatggct taaagtattg gtataactac
     7801 acgagtgatg acttaataaa ctttaaagct gaagggccaa ttttaaatcc agatactaaa
     7861 tatgacagcc atggtgtata tagcggtagc gcttttgaat ataacgggca tttatattat
     7921 atgtacacag gaaatcatcg agataatcat tggcaacgac atgcgagtca gatgatcgca
     7981 cgattgaaag aagacggttc agttgaaaag tttccaaagc cagtaattag ccaacaacca
     8041 gaaggatata cgagtcattt tagagatcct aaagttttta aatatgatga gaaatattat
     8101 gcaatcattg gtgcacaaaa taatgatcag caaggtcgat tattacttta taatactgaa
     8161 gatataatta attggcatta tttaggtgaa ataaatacag agttggatga ttttggatat
     8221 atgtgggaat gcccagatta ctttaatgta gataatcaag atgtcatact tatttgtcca
     8281 caaggtattg aaccaaaagg cgatcagttc aaaaatattt atcaaagtgg ttatatactt
     8341 ggaaagtttg atattgaaaa gttaacatat gaacatgaaa attttgtcga gcttgataat
     8401 ggttttgatt tctatgcacc tcaaacattt ttagatgaaa aaggccgacg agtactaatt
     8461 ggatggatgg ggttaccgga aatcgaatat cctactgata atgaaggatg ggcccattgc
     8521 ttaacaattc ctcgtgtatt aaatgtagaa aatggtcaac ttaagcaacg tccatatcca
     8581 gcgttggaaa aattacgtca caataaagag acagcattag gctacgcaaa taaatttact
     8641 cgaaaattac atccgtatga aggtaaacag tatgaattaa tcatagatat tttggataat
     8701 gatgctaccg aagtgtactt tgaattacgt acatctaaga cttcttcaac attaattgct
     8761 tataacaagc gtgaaaataa aataacatta gatcgcagcg acagtggttt attgccgaca
     8821 aatgttgaag gtacgacgcg tagtacgata ttagacacgc cattaaagca attacaaatt
     8881 tttgttgata catctagtat agaaattttc tgtaatgatg gagaacgtgt tttgacatct
     8941 cgaattttcc cgacagaaga cgcactaggt attaaaacat caacagaatc agggcaagta
     9001 tatttacaat ttactaaata tgatttaaag gatgaacata aatgagacgt ctattttcaa
     9061 taggagaagc gttaattgat tttattccaa atgttacaaa tgcgaattta aaagatgttc
     9121 aaacatttac gaagcaaatt ggtggcgcgc catgcaatgt agcttgtaca gttcaaaagt
     9181 taggacaaca agcgtatatg attacacaat taggtaatga tgcatttgga gatagcatta
     9241 ttgaaacgat atcaagtatt ggtgtagatg tatcaaaagt gtatcgcacg aatgaagcaa
     9301 acactgcgtt agcttttgtg agtttgactg aagcgggcga gcgagatttt tctttttatc
     9361 gtaagccctc tgctgatatg ttatttgaac caagttttgt gaatgatatt gatgtgaatg
     9421 aaaatgatgt agtacatttt tgttctgttg atttggtcga tagtccgatg agagatgcac
     9481 attatcaatt gattaccaaa acattaaatg caaatggtac tgtcgtattt gatcctaatg
     9541 taagattgcc attatgggat aacgctgaag atttaagaca aactattcat acctttttgc
     9601 cattggcgca tattgtaaaa gtttcagatg aagaattaga atttattaca ggtattcatg
     9661 atgaaaatga agccattcaa tcattattta caggtaatgt tactgtagtc atttatacga
     9721 agggagcaga tggtgctgcc gtatatttga aaaatggtat taatcattat catagtggct
     9781 ataaggtaaa acctgttgat acaactgggg gcaggggatg catttattgg tgcagttatt
     9841 agcaggattt tagcaaccga tgtgttaaat ttaactcagt tatttgaaaa tgaaggtgag
     9901 gaaattttag cgtttagcaa tcgcgttgct gcaatagtga caacgaagta tggtgctatt
     9961 aatagtttgc caacattaca agaggttgaa caagcatttt aaataattca tttataaaac
    10021 agtgtaggaa acaagttgaa tcttgtgcta cactgttttt taaaacaaaa taagattcac
    10081 ggagtaggaa attatagcat tttatttatg gaaactgtca atttcaaatt tagtatattt
    10141 tgttaaaaat tgcgccatag gattgtagag tgaaatagaa attgataaat tatgtagtaa
    10201 taggaatagg tatacaaaga gacgttacat cattttttaa ttcacgcgtc atatttaatt
    10261 ttgttaataa aattaaaaag ccagctatac agtgcatttg ctagttatct tgttaaaatc
    10321 caacaagatt tacaattgaa tacgccgtta actgacttta ttatcttatt atattttttt
    10381 aacgtttctc accgatgcat agcagtgttc tttattttta aaatagacaa ttcgctcttt
    10441 cgaatctata gattcaatat tatggcgatt gacgacaaag ctattatgac atctaaagaa
    10501 acgatcatct aattgactca gttcttttaa attaccataa aattcaattt gacggttatc
    10561 taaatgggca atgagtctgt gagattttgt tgatgattca aaaaacataa tatcatcata
    10621 ttgaacatac actgaattac tgccacgttt taattcaatc gtttcaacgc tattatcttt
    10681 agacaacaat tgtaagcgtg tatgtgcagt ttctaaacag tctataattc gagttcttaa
    10741 ttcagctgga tcatctttaa aaataaaatc catcgctgca actttgtaga caaatgttaa
    10801 ataggtaagt tcactgtgac tcgtaacgaa aataatgtta ccaactgggt catgcttacg
    10861 aatttcactg cctaatttga taccattaat atcagttgaa agttgaatat ctaaaaagta
    10921 acagcctatg tcattcatat ttttagcttg ctcaagcacc tcataaggat tatcagttgc
    10981 gagggcaatt tccataggct tttcttctat cattatataa tttttaataa tggtaaccat
    11041 gttttctctt tgttttggat cgtcttcgca aatgaaaatt ttcatacatt cacatcctta
    11101 tggctagttg ttaataattt caactttttg aataaagaaa ccattttcga taattgtatc
    11161 taataagaca ttgtctgcat tatcagcaat ttcttttaaa gttgatagac ctaaaccacg
    11221 accttcacct ttagtagaaa aactttcttg gaacaattca tgaatgcgtg gtatatcatc
    11281 agcgcattta ttcataacaa taaacgttac tgaattttca ctttcaataa atgcaacgcg
    11341 aatgataggg tcatcaattt cagttgatgc ctcaattgca ttatcaagaa taataccaat
    11401 actgcgactt aaatcgatca tattcaagtt aatgctactt acttcatcgg gtatttcgat
    11461 actaatcgga aaattcattt cttgtgcacg taaaattttc gcagtaatta agcctttaat
    11521 ttcacgtact ttaagattct cgataccatt taattttata gcattcattt gtaaattgtc
    11581 tttcataggt acaatatttt tattgaaata atcacgtagg ccaggcatgt catcttctcg
    11641 aatgtattct gaaagtgtcg ttaagatatt gacataatca tgacggaact tgcgcatttc
    11701 gttgttgata gcttcaatct tcaatgtata ttcataatag gtttcaattt cttcttgatt
    11761 acgtttatat ttcatctctt taaggagaaa ttgagaaata
//
LOCUS       DAKTGS010000091        11504 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.091, whole genome shotgun sequence.
ACCESSION   DAKTGS010000091 DAKTGS010000000
VERSION     DAKTGS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.091"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            474..1460
                     /locus_tag="O0286_001923"
     CDS             474..1460
                     /locus_tag="O0286_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0170295.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            complement(1950..2363)
                     /locus_tag="O0286_001924"
     CDS             complement(1950..2363)
                     /locus_tag="O0286_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170296.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(2392..2502)
                     /locus_tag="O0286_001925"
     CDS             complement(2392..2502)
                     /locus_tag="O0286_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170297.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            complement(2588..3319)
                     /locus_tag="O0286_001926"
     CDS             complement(2588..3319)
                     /locus_tag="O0286_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0170298.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(3321..4634)
                     /gene="murT"
                     /locus_tag="O0286_001927"
     CDS             complement(3321..4634)
                     /gene="murT"
                     /locus_tag="O0286_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCY0170299.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            4926..5426
                     /gene="ftnA"
                     /locus_tag="O0286_001928"
     CDS             4926..5426
                     /gene="ftnA"
                     /locus_tag="O0286_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCY0170300.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(5508..5600)
                     /locus_tag="O0286_001929"
     CDS             complement(5508..5600)
                     /locus_tag="O0286_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170301.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            5815..6369
                     /locus_tag="O0286_001930"
     CDS             5815..6369
                     /locus_tag="O0286_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCY0170302.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(6437..7507)
                     /gene="dinB"
                     /locus_tag="O0286_001931"
     CDS             complement(6437..7507)
                     /gene="dinB"
                     /locus_tag="O0286_001931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCY0170303.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(7754..8284)
                     /locus_tag="O0286_001932"
     CDS             complement(7754..8284)
                     /locus_tag="O0286_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0170304.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(8454..9815)
                     /gene="rlmD"
                     /locus_tag="O0286_001933"
     CDS             complement(8454..9815)
                     /gene="rlmD"
                     /locus_tag="O0286_001933"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCY0170305.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(9896..10843)
                     /locus_tag="O0286_001934"
     CDS             complement(9896..10843)
                     /locus_tag="O0286_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0170306.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         3693 a         2082 c         1626 g         4103 t
ORIGIN      
        1 atatactaaa gatgttactt tagtattttg gaaagtgagt gagacatagt gtgataaaga
       61 aaaaatattt ctttatcgtt gtaccccaac ttgcattgcc tgtagaaatt gtagatccaa
      121 tttctctatg ttggggcccc gccaacctgc acattatcgt aagctgactt ttcgtcagct
      181 tctgtgttgg ggccccgcca acctgcattg cctgtagaat ttcttttttg aaattctcta
      241 tgttggggcc ccggactata attgaaaaat gcttgttaca agtgcatttt atttcagtca
      301 actactaaca atataacatt gtggagccca gaactttgat taatgtatat gaaaatcaaa
      361 gtaatgctta tgtatgatta tttcaaatat tttacataca tgaacttttc caatgtacga
      421 tactattatt ataaagcgct cgctaagatt ttacgatgat tagaggttaa aaaatgaacg
      481 atcaatggta taaacattta attggtgcca gaacaattaa aactggtatt gccatttttt
      541 taacagctgt cttttgtatg gcactagatt taacacccat ctatgccatt ttaacagctg
      601 tagtcacaat tgaaccaact gccaaggcat cacttattaa aggttatcgt agattacctg
      661 ctacggtaat tggtgcaggc tttgcagtat tatttacata tttattcggc gatcaatcac
      721 cttttacata tgcattgagt gcaacgttca cgattttatt ctgtacaaaa ctcaaattgc
      781 aagttggtac gaatgtcgct gtactaacat cattagctat gattccaggt attcatgatg
      841 cctacatatt caactttttg tctcgaacat tgacagcaat tattggactc gtgacatcag
      901 gtttaattaa cttcatggtg tttccaccta agtattatgg tcaggtcgaa gaaaaattaa
      961 gtaagacaga tgccttgatg tataaattat tttacaatcg ctgccaagaa cttatcttat
     1021 caagactgca atctgataaa agcgaaaaag catataaaaa tattttcaat ttaaataatc
     1081 aagttgaaac gttaataagt tatcaacgtg atgaattaag ctatcataag aaaaaagaat
     1141 gtgattggaa attactcaat caattaacta aacgtgcata tacgaatcgt ttgtttatca
     1201 ctcatttatc aaatatcatt tacttgccta aaaatactcg tgttaacttt tcaggtgacg
     1261 aaaaaatggc attgctaaaa attagtagta gcatcaaaga tatattttat gatggttctt
     1321 ttaagagaga agatgattct gtagaaactt tacgttcgac tataaaagca cttgaaatta
     1381 gtggtgaaaa tcaaattaaa agtcatatac tatacgaagt tctaatgatt tatcgactgc
     1441 tagacagcag atatgcttaa tttaagtacc ttatgctaat tggcaccatg ggagtgggac
     1501 agaaatgata ttttcataaa atttatttcg tcgtcccacc ccaactcgct ttgcctgtag
     1561 aatttctttt cgaaattctc tgtgttgggg cccacacccc aacttgcatt gtctgtagaa
     1621 attgggaatc caatttctct ttgttggggc ccacacccca acttgcattg tctgtagaaa
     1681 ttgggaatcc aatttctctt tgttggggcc cctgactaga attgaaaaaa gcttgttaca
     1741 agcgcatttt cgttcagtca actactgcca atataacttc gtagagcata gaacattgat
     1801 ttatgtcccc gcctcttttt atcgtatata gcatgtccct ttttaaaatt acaagcataa
     1861 atattcagac tcaaatatag ccacacgcct aaactacgga gggatgtggc tgtctttttt
     1921 gattgtaatt acatatcaga tttaaatcat taatgtaaag aggctaattc attaataaat
     1981 ttagtaaaat ctgatttagc aacatttatc cttaatgaaa taccagattc tgttgccatg
     2041 tttgaatgcc ttaaaccaga atcaaaatca atataaatac aaagcagttc ttgattaaaa
     2101 tcaataattg taaaatgtaa atcaggttct ataaaatata tttcagtaac aggattgtcg
     2161 caatccatat gtttcaaatt gtccatgtca ctatcaaaga gtgcaaattc aaatttaact
     2221 tcttgatctt cgaaagttgc actagtgtcg aaataataaa ttttattatc ttcttcgata
     2281 actacatctt ttaaaataaa tgttatcgat gttttgctat gtaaataaga tgcatctttt
     2341 aatatcactt tagtaggctt catcgtcaaa ttcctttcat cattcatttt ttcatgttgc
     2401 ataatcacca ttactattga taattctagg atagatactc attgtataat aattattcac
     2461 tagaacaaaa aaacacacat caatttggtt gtttccatcc aaaataatgt gtgtgtttat
     2521 acatatatga ttaattacta aacttcatcg tttatcaata ttaaatccat tgatgcgatg
     2581 ttcagagtta acgagatttc ttctgtctat ttgctctgtc tattaatact tgtttcgctt
     2641 gtatttccgc ttcattatct atttctttag gctcaaacgg aataccctta cgttcacaag
     2701 ctttttctaa cagataatca gtgatttcgt aatttttagg taaaattggt ccatgtaaat
     2761 aagtacctaa taaattttta taatgaatgc cttctttttt atcttcatca ttattaccat
     2821 aaccaaaagt aacatgacca agtgtaccga aatcatgata tgttctacca ccgtgatttt
     2881 caaaacctac aatagttcca aaagtatcac tttcgataac aatatctcct gttaatcggt
     2941 ttgtctttga ttcagtataa aaatctaaaa tacctaaccc ttctaattct gtaccatcag
     3001 gcgtgatata ttttttccct aaaaattgat agcctccaca aatcgttaat cccggcatac
     3061 catcttcaat cgcttcttta agtggtgtct taattttact taattctttt gttgctaatg
     3121 cttgttctct atcacttcca ccaccgataa agaaaatatc acattcatca aaggtaatac
     3181 cttctgtttc attgatttct acgacattaa ctttaatatt tcgtttttta gcacgttgtc
     3241 ttaaagcaat aatatttcct atatcactgt ataaattcaa tttatctgac ataaaatgat
     3301 aaatagtcaa ttcatgcata ttatgattga cctccttcaa acgaacggtt taattgttca
     3361 agcataggcg ctaatgatgt atagtttggt attgcaactg tgaaaccttt ataatccata
     3421 gtctttgccg ttgctttata aatatcacgc tcaactataa ttggtacttc aacctctgct
     3481 aacttcaatc gcaattgaag ttcttctgct cgtgtacctg tcacgatgat agcttcaatt
     3541 tgttgcttag ataatttttc aaaatctgca tcataaatcc atgaagtatc tcgaccatct
     3601 gcagcgttat catttagcga aataacatac actttttcgc cttctaattg ttcaccaact
     3661 gataaacttg cattcattcc tgcaggattt ttagctaaat tgatcatcgc ttcttttcgt
     3721 tcttttttaa agtactgcat acgaccattg tctgatgtat acgtttcaaa gccattttta
     3781 attgtttgtt catttaaccc tagctctctt aaaacagtat atgctgctaa cgcgttataa
     3841 gcgttaaagt cacctgcaat tttcatatca tatttttcat cattgatatt taaatataaa
     3901 aacggtgcca catcaaaact tgatatttca tattttgctt gctctcgttt gaaaccacac
     3961 tgacagtgat aatgaccaat ttgattataa tgaatataat cgtattgcaa taagcgacca
     4021 cagtttggac aatatctact ttcattcatc gtactttgtt caaattcatg ggcatgtgct
     4081 ttcataccat agtacacaat cgtatcactt gcgattttca aacgactcac aaatggatca
     4141 tcagcattta gcaataattt gatgccttta ttactaattg tctctgcaat gttattaacc
     4201 ataatatcaa tttcaccgaa gcgatccatt tgatctctaa agaaattagt aaataccatc
     4261 attgaaggtg taacttcttt taacacacgt ggaatcgaac cttcatcaat ttcgattacc
     4321 gcaattttag tcttaggtgt tgattgcatg atgaatgcag aagttatacc tgcagccata
     4381 ttagcacctt cattattgtg tataatttga atattatttg cttttaaagt atgtccaatt
     4441 aagtttgaag tcgttgtttt accatttgtt ccactgataa atacaatatc atcaacttgc
     4501 tctgctaatt ttcttaatat atctgtatcc acttttctag cgatttgtcc aggtaaatct
     4561 gttcctcttt tacctactgc tctacttgct ttacgcgcca atttcgctag atggattgcc
     4621 gtccactgtc tcatgtgttt cctcctcaaa tttccactcg catcattata acatgacaag
     4681 gcaacttcaa aaaagtttct caatcacaaa ttgatacagt gtttctaaat tataatcatt
     4741 ctcatttaga taattaaaaa tgatatttat tctcaattaa atatcgctaa atgatacatt
     4801 ttctcaatta gagcgctaaa ataaggcatt aaattataat tattattatt tacaattgag
     4861 aatgagtttg ttatactaag tttaattcat agtaatttta atttacaaat aagaggtgta
     4921 tcaaaatgtt aagtaaaaat ttattagaag cattaaatga tcaaatgaac catgagtact
     4981 ttgcagcaca cgcatatatg gcaatggcag catactgtga taaagaatcg tacgaaggat
     5041 ttgcaaactt cttcattcaa caagctaaag aagaacgttt ccatggacaa aagatttata
     5101 actatattaa cgacagaggt gcacatgcag aattcagagc agtttcagca ccaaaaattg
     5161 acttttcaag catactagaa actttcaaag acagcttatc tcaagaacaa gaagtaacaa
     5221 gacgtttcta taacttatct gaaatcgctc gtcaagataa agattatgca actatctcat
     5281 tcttaaactg gttcttagat gaacaagtcg aagaagaatc aatgtttgaa actcacatca
     5341 attatttaac tcgtatcggc gatgacagca atgcattata tctttacgaa aaagaacttg
     5401 gcgctcgtac attcgacgaa gaataattaa acatcactac aatagacaga taaatatcat
     5461 acgacatgat aggcatttgg gtcacttaca ataacccaat gtctatatta ttttgcttta
     5521 cggagatcac tagattcatt ttctgaatca ttgatctgcg ttttttcatt ttcaaggcta
     5581 attattgtat ttttagtcat ttatttttta aactactaat gttaataact ctaaatttga
     5641 tgttgaatta atttgacgat tttaaagcat atcatcattt actttttaat cagagttaca
     5701 tccaaatgat agatttcacg ttataccttc acgtataata ttatgtatcg tttgtaagca
     5761 aatgactaaa agtctattaa tatatacatt taattaattg aaaggattga ctacatgata
     5821 caagatgcgt ttgttgcact tgattttgaa acagcaaatg gtaaacgtac aagtatttgt
     5881 tctgtcggaa tggttaaagt cattgatagt caaataacag aaacatttca tactcttgtg
     5941 aatccgcaag actatttttc acaacaaaat attaaaattc atggcataca accagaagat
     6001 gttgaaaatg cacctacgtt tgactacgta tttccatata tgatgcaatt tattgcagat
     6061 ttacctgttg tcgcacataa cgcggcattt gatatgaacg tcttacatca aagcattcaa
     6121 aatattggtt taccaactcc aaatttaact tacttttgta gttatcaact tgctaaaaga
     6181 accgttgatt cgtatcgata cggtttaaaa catatgatgg agttttatca attagatttt
     6241 catggtcatc atgatgcatt gaatgatgcc aaagcatgcg caatgattac ttttaggcta
     6301 ctgaaaaatt atgaaaattt aacatatgta actaatattt atggtaaaaa tctaaaagat
     6361 aaaggctagg actaaataaa atactccctt caaaagtaag cattgtaaaa atgtaaactt
     6421 tgcagggagc tttattttat ataaagtcat atatcgtcat atttttataa gttgattgtt
     6481 ctaaattacc tacagtgaca ccaataagtc gaattggtac atcagggtct tttaaatcgt
     6541 tataaagtaa atatgcaata ttataaatat cttcttcaga actaaccgaa tctcttaaac
     6601 tcatctgttt agatagcgtt tcaaattgat aagttttaat tttaaccgtt acagttttag
     6661 ctgacttctg taatttattt agacgttcag ctgttttacc tgacaattcc catacttttc
     6721 ttaaaatctc ttcatcatca ttcacgtctg ttgcaaatgt gcgttcagtc cctactgatt
     6781 ttcttactct tgatgatttc acttcactat ggtcaatacc gcgtgccttg ttatataaac
     6841 cccgacctct ttttccaaac aaacgtatta attcaaattc cgttttctca tataaatctc
     6901 taccgttaaa aataccatta tcatgcatta cttttttgga agctttacct acgcctggaa
     6961 aatctccaat atccaatgtc atcaaaatat catggacatt ttgataatca atcacagtca
     7021 taccatcagg tttattcata ccactcgcta atttagctaa aaatttgtta taagaaacac
     7081 ctgcagatgc tgttaaatgt gtctgctcta gaatatcttt tctaatatac tgagcaattt
     7141 tcgaagcagg aaggtctggt ctcactaatt ctgtaatatc taaatacgct tcatccaatg
     7201 acatcggttc taccttatct gtataacttc ggaaaataga cataatctgc gcagatgttt
     7261 ctcggtaagc accaaaatta cttgtgacaa agtatccatt tggacataat ttatgcgctt
     7321 gtgacatagg cattgctgaa tggacgccgt attttcgtgc ttcataggat gccgtagaga
     7381 caacacccct actgcttgct ttaccaccaa caatgactgg tttccctttc aatttggggt
     7441 tatctctcat ttcgacttgt gcaaaaaaat agtccatatc tatatgaata attcgtctct
     7501 cagtcaagtg ctcacctccc tactaatttt tacttttata acgcacaaaa atatctcaac
     7561 ataattatac gctgtgtacg atttttttac ataaatcttg cacttagcga taactatatt
     7621 gagataacta caagttgtta taaaatcaat tgctatttaa gcatgatgat gaagacgatt
     7681 gagtaagaaa acataggtaa tctgaaataa ttcaagcaaa ttcattttgt tggtatcatc
     7741 atattaaaat ttattattga gtcggctttt gatgatacaa ataaatacta tcttcatctt
     7801 gttgaacata tttaaaacta ctagatattg ttaatgcaac aagcagaata tctataaagc
     7861 aataagcggt atgcatacta aacataaaaa taagtgatga ataaccaaat accttaatta
     7921 aaataagcaa gccagtactt aataggatta gtggtgacag cataataatt gagaattgcc
     7981 atttgttgaa gcaagcatct gctgtttgga ataagattct gtcttttttt atattaaaca
     8041 taggtttgct atctttttta aataaaagaa ataatgctct atggataagt tcatgtaaaa
     8101 tcaataaaat aatgaatcca gcaaacccat atacaagatt gatgatgata ttttgatcga
     8161 caaccgctgt gacacctaac gcccacttat acgtaaataa aatcacgaat aacgcaataa
     8221 caagttgcaa gataataaac cttcgcattt gaaaattatt tgtcgttaaa tcaattttat
     8281 gcattaccaa ccctcccgat catgacattc ttattcttct ttaaatatag tatacaatgt
     8341 cacatttaat ttaaaaagtt catatcaaga aagtaaattg gctgtaataa aattttaata
     8401 tacgacttct ttcttcactt attaaggcga aattttatct caaatcatgt gcgctatttc
     8461 aaattgaata atgccactgt ctcaacatgt gttgtttgtg gaaacatatc taccggtgtt
     8521 acctcttcaa gttgatattt ttcagctaat aataatgcat cacgttgctg tgttgcggga
     8581 ttacatgaaa tatagacaat acgcttaggt tctaatgtaa gcaaagtctg aataaacgtt
     8641 tcgtcacagc cctttcttgg cggatcaacc attacaacat ctggtttaat cccttgtgct
     8701 ttccattgta aaataacttc ttcagctttc ccacagacaa aagttgtatt attgcattgg
     8761 tttatagtcg cattttgttg tgcgtcttca attgcagaag gtactacttc aacaccgtat
     8821 acatgttttg caagtggtgc catatatagc cctattgttc caataccaca ataggtatct
     8881 aatacaactt cattacctgt caattgcgca tactcaattg ctttattata taatttctct
     8941 gtttgttcag aattaatttg gtagaatgac tgatcactta ttttaaatgt actatctgtt
     9001 aattgatcaa taattgtatc tttaccatat agcgttatag attgacgtcc cataataaca
     9061 ttagagtggc tatcattaat gttttgttta atgcttgtca cattaggaaa tgcatctaat
     9121 atcttctcaa caacagcatt tttttgtggc cactttttac cattagttac aaaaataatc
     9181 atcatttcgt ctgtatgata tcctgttctt acaaccaaat gtctcattaa accttttttc
     9241 aattgttctt gataaatact tacatttaaa tcttttaaaa tagatttaac ttcattcatc
     9301 acttcttgat gttgtgaatc ttgtattaaa caactttcca tgtcaataat gtcatggctt
     9361 ctttgacgat aaaagcccat aataacttca ttctgttcat tcttaccaac tggaatctgg
     9421 gacttgtttc gatatctcca aggatctgtc atgccaactg tatcgttaat cttagaatta
     9481 tcaaaatgcg cttttcgctg aaacaaatta atcacttgtt cctttttcat ttcaagttgt
     9541 gcttcgtatg ataagtgttg aagttggcac ccaccacaac gttcataata tatacatggc
     9601 ggtgcaacac gattctcact tctcgtattt atattaagca acttacctat cgcaaagttc
     9661 ttttttactt tgataatttt atattcaatt tgttcattaa ttaaagcttg tggtatgaaa
     9721 ataggaaagc gatctatttt tacgacacca tggccttcat gcgttaaatc aacaactgtt
     9781 cccgttttta tgtcattttt agctattgct tgcaaaattt tacctccaaa atgaacaggt
     9841 taggaacaaa attatgcgct tcctaacctg ccattatata tttcactatt tctgtttatt
     9901 cttcgattaa attgtcatca acatgatcat tatttattaa ctcttcattt acaatatcat
     9961 taggtgcaaa gacatctata tgacgttcta gatttaagaa attcgctggt aatttaccac
    10021 catattctcc atctacattt agttgtaagt ctgtgaatga tgaaatatta attgcctttg
    10081 ctttttcata aataacttta ggatgcttag tatgttctcc tcttgaagct aaagtcataa
    10141 tatgaccaag ttctgcaagg tttgattttt caactataat taacgtaaaa tagccgtcat
    10201 ctaacttagc gtccggcact aatttttcaa atcctgccat tgaatttgtt aaacctaaaa
    10261 agaataataa tgcttctcct tggaaaacat taccatcata ttcaattctt aaatctacag
    10321 ctttcatttg aggtaacatt tcgaaacctt tgatgtaata agcaaatgga ccaacaatag
    10381 atttcaattt actcggtgtt tcataagaga cttgcgtcaa ttgtccgcct gcagctaaat
    10441 taataaagta tcgattattc attttaccaa tatctacttt agtagaatga ccttcaatga
    10501 tgacatcaag tgcccccatg atgtcattag gtatatgcaa tgcacgtcca aagtcattaa
    10561 cagtacccat aggaatgaca cctagcttag gacgattagg cttttctgcg ataccattaa
    10621 ctacttcatt taatgttcca tcaccacctg cagcgattaa tacatcataa ttttcatgca
    10681 tagctctttc tgcttcaagt gtggcatcac ctattttctc ggttgcatat gcactcgttt
    10741 catatcccgc tttttctaat tttattaagg catcaggtaa ttctctttta aatagctctt
    10801 tacctgatgt cgggttataa atgattctag cacgtttcct catatcttat ccctctactt
    10861 aaaattcata tattttaact tcatctttgt ttcgtctaat agggagtggg acagaaataa
    10921 tatttaacaa aatttatttc gttctacccc aacttgcatt gtctgtagaa tttcctttcg
    10981 aaattctcta tgttggggcc ccaccccaac ttgcacatta ttgtaagctg acagaaagtc
    11041 agcttctttg tttgggggcc ccgccaactt gcacattatt gtaagctgac agaaagtcag
    11101 cttctatgtt ggggccccac tagaattgaa aaaagcttgt tacaagcgta ttttctttca
    11161 gtcaactaca gccaatataa cattgtagtg cctaggacat tgaatttatg acccaggctc
    11221 agtcttattt catcattctt aatatcgtta aagaccaact tgtatcttaa acaaatacta
    11281 tctcaatatg tacaaagctt gttatttatt cagcattttt tgccgttctt cattatatag
    11341 acttcgtcag ttatgctatt ttacctttaa aatgatgttg taaatataat gttgtcttta
    11401 tacaaaaatg ttttatacct ttttattata cagtttgcct taatataatt aaagctaacg
    11461 cattaataaa attaatattt ttaccattaa catgtacaat gaat
//
LOCUS       DAKTGS010000092        11373 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.092, whole genome shotgun sequence.
ACCESSION   DAKTGS010000092 DAKTGS010000000
VERSION     DAKTGS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.092"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..461)
                     /locus_tag="O0286_001935"
     CDS             complement(<1..461)
                     /locus_tag="O0286_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0170307.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVN"
     gene            complement(458..1267)
                     /locus_tag="O0286_001936"
     CDS             complement(458..1267)
                     /locus_tag="O0286_001936"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCY0170308.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(1284..1919)
                     /locus_tag="O0286_001937"
     CDS             complement(1284..1919)
                     /locus_tag="O0286_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0170309.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(1936..2283)
                     /locus_tag="O0286_001938"
     CDS             complement(1936..2283)
                     /locus_tag="O0286_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170310.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            2469..3062
                     /locus_tag="O0286_001939"
     CDS             2469..3062
                     /locus_tag="O0286_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCY0170311.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            3166..3531
                     /locus_tag="O0286_001940"
     CDS             3166..3531
                     /locus_tag="O0286_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCY0170312.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            3554..4360
                     /gene="yjbH"
                     /locus_tag="O0286_001941"
     CDS             3554..4360
                     /gene="yjbH"
                     /locus_tag="O0286_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCY0170313.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(4820..6628)
                     /gene="pepF"
                     /locus_tag="O0286_001942"
     CDS             complement(4820..6628)
                     /gene="pepF"
                     /locus_tag="O0286_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0170314.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(6676..7661)
                     /locus_tag="O0286_001943"
                     /pseudo
     CDS             complement(6676..7661)
                     /locus_tag="O0286_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(7782..8501)
                     /gene="mecA"
                     /locus_tag="O0286_001944"
     CDS             complement(7782..8501)
                     /gene="mecA"
                     /locus_tag="O0286_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCY0170315.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(8872..9267)
                     /gene="spxA"
                     /locus_tag="O0286_001945"
     CDS             complement(8872..9267)
                     /gene="spxA"
                     /locus_tag="O0286_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCY0170316.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            9562..10551
                     /gene="trpS"
                     /locus_tag="O0286_001946"
     CDS             9562..10551
                     /gene="trpS"
                     /locus_tag="O0286_001946"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCY0170317.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(10593..>11373)
                     /locus_tag="O0286_001947"
     CDS             complement(10593..>11373)
                     /locus_tag="O0286_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170318.1"
                     /translation="VSIIAPLIAPFPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRL
                     LYAGRISLSIGITSTIGMLLIGITVGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIV
                     LNAALGDKIKNPYGSAIILVLVIIVLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPT
                     YKIILKHLLPNILSVVIVQATLLFAGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEG
                     DVISGKPWIWMPPAIMITLTILSINFVGEGIKDAFNPRGRR"
BASE COUNT         3844 a         1932 c         1598 g         3999 t
ORIGIN      
        1 aagtttactt tagaaaataa atgatggata tgtccatatt tagcgaatat cacaatacca
       61 gttgtattac gatctagacg cgtaacaata tgtgggttaa tattttcacc atgttcctga
      121 caatgatata gtacttgttc gattaaactt tcatgaggat gttctctcga aggcgtacaa
      181 ttttgattgt ttggtttagt aactatgatg ataaaagcat cttcatacaa tacttctagc
      241 ttacgagcat aaggtattaa attaacactc ggtatttctc gcggtaaatg aatttctaat
      301 atatcatttg tcattaattg cttacgcact gtgactggtt catcattaac aattaaagcg
      361 ccattatttt taatggcgct cactgtcttc ttagaaaaat catgtcgtgc taaaaaagtt
      421 ttaacagttt cttgttgtga tatatgatac ttaaatttca tcgttcttca tcacttgata
      481 tgaaagaatc gtgcacacgt ttccagaatg ggaatggtct aaaacgtgca aacctcactt
      541 tttcatttgc tacacggtat tgtattgaat taacattttt atgtttgata ctaacatgat
      601 ctatcgtcat tctaatggta tcatgattaa ctggtgatat taaacatgta tgatgctttg
      661 gtaatacaag tggtgatcct accgttctaa acacacgatt atttatcgag gcaatttctg
      721 taatttgcat tgcttcaagt gaaggatgta tcagtgcgcc acctagcgct ttgttataag
      781 ccgttgaacc cgaaggtgtt gatacacata atccatcgcc tctaaatcgc tcaaagtgtt
      841 tccctcttaa gttaacatcc acaacaagtg ttgagccatt ttcagttttc atcgttgctt
      901 catttaatgc taaatacctt gtttcatagc cgttgtcgtt gtatctcata ataatttcaa
      961 gcaatggata ttcaatgacc tgaaactctg aattattaat ttcgatgatt aatttttcaa
     1021 cttcatgagg taaccaatcc gcataaaatc ctaaatgacc tgtatgaact ccaacaaatg
     1081 ccacttttga taacatgtgg ctatactgat ggaatgcttg tagtaatgta ccgtcaccac
     1141 caactgaaat aacaatttca ggattttcac tatcctcaat catgcgaaaa tctttcatat
     1201 agttcatcat tttatgcttt aaggcattag acttggagtc acctttagtt aaaattgtat
     1261 aacgcatgat ttataacacc tcgttaatca ttttcatgtt ttttagaacg tttttgcgtg
     1321 taatattttt gagcttcctg aatttcatct ttaatttcag acatctcttc atcaagtaaa
     1381 tacgctgctt ctgccgctct ttccaaacga tgttgaattt catccggata agcaccatca
     1441 tatttatatc gtaaagtatg ttcaatcgtt gcccagaaat tcattgctaa tgtacgaatc
     1501 tgaatctcag ccaatataaa tttttggcct tgtaatgttt caattggata ttcaataatg
     1561 acatgatacg agcggtaacc actttcttta gtgttacgaa tataatctcg ttcttcaatt
     1621 actttaaaat cttttctttg tcttaaaata ttgacgacaa catcaatatc ttcaacaaat
     1681 tggcacatca ttcttaaacc agcgatatcg tacatttctt ctcttaacct atcaaatggt
     1741 atttgtcgtt tgtttgcctt atctataata ctagcgattg gtttaacacg accagtaaca
     1801 aattctattg gcgacgcttg ttcaccaact tcatattgtt tgcgcatgcc tttaagtttc
     1861 actttcaact catcaaccgc ttgcttataa ggtgttaaga actgatccca ttgattcata
     1921 gtgcttcacc ccgctttatt caatgtcgaa tgtttcttca acggttgcaa caaattcctt
     1981 accataatta ttattccctt ttatgttgtc taaaatataa gtaagttctt ggtggaattc
     2041 agtcaattct agttcatcat tgataataat ttttttagtc atgttttcat gcaacatcgt
     2101 ccatgtttct tgtacgaaaa tgagataagc atacgcttta ccatcatcta atgtatacgc
     2161 aaaggcataa ttatcactat caacgagcat ttgtcctact tcagataatc ctttaataga
     2221 atcttctgta taacaataaa gtatgtcatc tttaatttta atttcattaa tatataaacg
     2281 catgcttaat cctcctctta ttccataaca ttttaacaca attcaataat acaaaacact
     2341 tacgaacact atttttaatg gtcaaattaa cattgataat agataaagga aattactttt
     2401 aaaattcaac tttgtcattc ataatatagt taagataaat tgaatttgat acgaggtgta
     2461 tacatatcat ggcaacaaat catgaaatag aatttaaaca aatgataact gcatcaatct
     2521 ataataaatt gcaagagaaa tattttaaag acagtgtgct ttttaagcaa gtcaattact
     2581 atattgatac acccgatttc aaattaaagg aacatcgctc tgcactacgc ataagagtta
     2641 aagacaatca atatgaaatg actttaaaga caccagcaaa ggttggtcta ttagaataca
     2701 actatatcgt cgacattaaa ccagagatga acttgattat ttcaaacgac aatcttccag
     2761 atgacattcg tcaaattata gttgaacagt ttggtgtaaa agatacaact ttatcaatac
     2821 ttggtgcatt aacgacatat cgccaagaaa ctaaatataa aggtgattta ctggttttag
     2881 ataaaagtga gtatttcgat actacggatt atgagttaga gttcgaagtt aaagactata
     2941 atcaaggttt acaaaagttc caatctttat taaacgaact aaacctcgaa catcatcaac
     3001 ctttaaataa agttcaacgt tttttcaaga aaaaagaaac actttcaaac aatataaatt
     3061 aaattggcaa atatgcatat ttctttcaaa tgtttaaaat catgttatat tagttatata
     3121 ttaacttaag ttcgtaacag taaatttttg aaaatgggtg attaaatgac aacaacacca
     3181 tatgacatca ttggtaaaga agcgttatac gatatgattc attattttta cacccttgta
     3241 gaaaaagatg aacgacttaa tcacctgttt ccaggagatt ttgcagaaac aagtcgtaaa
     3301 caaaaacaat ttttaacaca gtttttgggc ggtccaaaca tttataccga agaacacgga
     3361 catcctatgc taagaaaaag acatatagat tttacaatca ctgaatttga aagagatgca
     3421 tggttagaaa atatgcagac agcaattaat cgcgctgcgt ttccacaagg tgttggagat
     3481 tatttatttg agcgcttaag attaactgct aatcatatgg tgaattccta aaattaattg
     3541 taggtgaata aacatggctg gagaattacg aataatggaa aataagagtc gtgaagatat
     3601 caatctatca cctgtaagta aaatcgaaat ttattcattt ttcgatccat ttagctccga
     3661 ttgcttcaaa ttatcagcaa tcttatccaa attaagaatt gaatataatc aatatatacg
     3721 tatcagacat atattaaatc cttcgttaaa agtattaacg aaatgccaag ctcaaagtac
     3781 atccaacttt gataacatcg ccctagctta taaagcagct gagttacaag gtcgtgtacg
     3841 tgccgaacga tttatacatt taatgcaaaa tgaaatcata cctaaaagag atattattac
     3901 agaatcaatg atttgtgact gtattcaaaa tgcaggtatt gatttagaag tatttaaaga
     3961 cgacttacaa aaaagtaaac taaccgaaag cttgaaaatt gatttgcata ttgcaagaga
     4021 aatggaaatc gaacaagccc cttctctcgt tttctttagt gaagatgttc atgaagaagg
     4081 tttaaaagtc gaaggattat acccatatca catctatact tatataatta atgaattgat
     4141 gggtaaacct atcgaaaaga atcttcctcc taaattagaa acttatatac agcaacaaca
     4201 acttgtaacg atggaagaat tacttactat ttatgaatgg ccagaaaaac ttttaaacaa
     4261 agagttaaag aagttagcca ttcaacaaaa aattgaaaaa cttaaatatc cagatggcga
     4321 tttctggaaa tctaaaatgc ctaaaatcaa atcaaaataa ataaattaaa gacctcgtac
     4381 accataaatg taacgaggtc tttttactga ctcttaattt tgcaacatta aattattaga
     4441 acgtaatgtt acacatcgat ctaaatatat tagtgatatt tttcgaattt aaccagttcg
     4501 aaaattatac aaattataaa tttggtacat aatagacata tatgaatcgt taatgtataa
     4561 aaagtgcata tttaatacat ttttatacaa cttcacttcc ctcttattaa aaaagaacat
     4621 aaaaaaacgt cgtgtctgaa tacacgacgc taaacaaagg ggatgggaga aatttttcac
     4681 ttcaaacaaa ggggtatgtt tgttatgtga ttaatttcat gttcataata taacatgcta
     4741 tctctaccat ttcaaccttt taatagtact taatttaaat tgtcacaatc tgttcataat
     4801 gtaagcgaat acaatagtac taagctttca ttaatttttc aaaagcgttc aatttttgtt
     4861 caaaaacttc acaagcttgt tcaattggtt caggtgttgt catatctaca ccagcatttt
     4921 ttaatatctc aattggataa tttgagctac cttttttcaa gaattcatta atatatctat
     4981 ctactgctgg cttaccttct gttaaaattt gatgacttaa gctttgagct gcactgtaac
     5041 cagttgcgta ttgatataca taataattca tatagaagtg tggaatacgt gaccattcct
     5101 tactaatatc ttcatcagtt tctacagaat caccgaagta taatttattt aatttggcat
     5161 attcttcatt cattctagtt ggcgttaatg gttcacctgc ttcttcaatt gcatgaattt
     5221 tatgctcaaa ttctgcgaac attgtttgtc ggaataatgt agctctgaaa cgttctaatt
     5281 cttggtttaa taataataag cgtttttcat catcaagatg tttatccata taatcactta
     5341 aaagtgcttc gttacaagtt gatgcaactt cagcgacaaa aatagtgtag tcactagaat
     5401 ttgacggttg gaattttcta ctgaagtaac tatgtgctga atgcccaaat tcatgaacta
     5461 atgtgtataa gtctgaaata gtattagacc agttaagtag aataaatgga ttagttaaat
     5521 gtgcacctga tgaatagcca cctgaacgtt tacctttatt ctcatagaca tcgacccaac
     5581 gattgtttaa gccttcttta actacattta aatattcttc acccattggt tctaatgctt
     5641 ttaacatcca ctctttagct tcttcataag gcatttcaaa cttaatatct ttaattaatg
     5701 gtgtatataa atcatacatt ttcaagtcat ctaaacctag caattctttg cgcaattcag
     5761 tatatctatg tagcaatggt aaatatttat gtacagtttt tactagattg tcatatacat
     5821 tttctggaat atgattatta ctcaatgctt tttctcttgc agttttgtaa ttgtgtgtac
     5881 gagcattaaa tacatttttc ttcacttcac ctgctagcgt agcgccaagc gtattattat
     5941 gagcaccata tgctttatat acatttctaa aagcactttc tcttagtttg cgatcatctg
     6001 tttctaaata cttaataaat gtaccttgtg ttaacgggtg tgcatttcca tctttatcta
     6061 tcgcatcttc aaatactaaa tcagcgttgc taaacatacc gtatacattt gatggcgttg
     6121 ataacgcgtc ctgcgcttct gttaataact tttcagtttc agcatctaaa atatgaggac
     6181 gtttttcatt aatcaacttc aaatcaaatg cgaatttttg taatttatca tatgaattta
     6241 caaatgattg aattttatct tcatcaattt gtaaaatctc tggcactaag aaactccatg
     6301 ccgagctaaa tttaataatt aattgatgtg ctcttgactc cataccagta tacttgtcgt
     6361 tcgttgtatc ttggtcttgt tttaggtgcg cgtatacata tactttttct aatttagtac
     6421 ctaatgtatc ttctaattct aacgcattgt ataatgtctc agcactatca ccaatgtgtc
     6481 ctttaaattg ttcttctttg cctaactcat tttcaacttc tttaaatgca gcctcaaaag
     6541 cttcatcatc tttgaaaatt gttgttaagt cccatgtata ttcaggatat ttacgttcct
     6601 gttcttctct cgataattgt tgactcatat gtagtaaacc tcctaataaa atataattga
     6661 tataattttc tcattttaga acaaaatttg cacgtcatta cctttatctg aacaaatatt
     6721 taaataattg ttaataagtt tttctataat tgctgtttta ctttctagag caacaataaa
     6781 ttttttcagt ttaaaataat taagattgtc atttactaat ttgctttttc cattctttaa
     6841 ccataaatca acttgtaatt gccactcaat aggatgattt ttaatataaa tttgctctgg
     6901 aaaaatataa ccataattgt gcactacttc ttgatcagtt aactgtaatt gatacattgc
     6961 acttaaagtg ggttctaaaa cagaattttg ccagcgacaa tatttgatat attgattaat
     7021 tgcgaacttt gataatttat aaacagcatg ataatcacaa ggcgcctcat taaacaactc
     7081 actaattttg gcatttcttt tttcagcgac atatttgtgc ccgcctaaat attgtatttg
     7141 ttgaaacatc attatttgtt ttttctctaa attgaacgta tgaagttttc gagtgaatgg
     7201 attaataaac atactttgaa aatgattgaa cttcacttta ttatgacaat aatcaacatc
     7261 atttaataac caacttactt tatatcccat tttctttaaa ccttccgttc gttgaagaat
     7321 ctgtttataa ggaattggcg aatactgtag ctcaataaca tatttattat taatcaaaat
     7381 atccggaatt tgttttattt cttttaaaat ggctctatct ctactttaca tccttgttgc
     7441 tgtatcatct gtgctaaaat caatttactt ttataatgtt taatcgtttc attattaaaa
     7501 catttttgtt ccgctaaatg tttatgtgca aaatgactaa ttactttgag cccacgcttt
     7561 aaaataactt gcttgccaca caccggacaa aaatattgtg tctttctcaa tgcagtagtt
     7621 gctaatacgc gttccttttc ttcatttaaa gctactaaca tatcttcacc tcaaaatatt
     7681 acacgtaagt ttaactacta ttactttttc agcttttgaa atcaataaaa aaagagactc
     7741 caaagagtct cctttcatag tgcgttgctt aattaatatc gttattcagt tgtctctgga
     7801 aaataacgtc gaacttgagc tgttacgtta tgactcataa ttattttagc atagtcattt
     7861 aaataaactt ctgttctgtc tgttggataa gcaaattcaa gcaattgact gtaactatca
     7921 ttaatgactt cttgatcaac atgactatca aaatatacag cataataata agtaccatca
     7981 accatatata acaaatcttc aaactctgta gttattggat tgctatgata tgcataatta
     8041 ataacatctt ctaaatcgtt aaatttcaca atgattgttc ttgtattttt acgtgctgaa
     8101 gacttttgac gtttagaacc ttgagcttct ttttcttttg ttcgttgctc gaataattct
     8161 tctaattgat cttcaccttc taatgtttga gctaacaatt cttgaacttg ttcatcaaat
     8221 tgatcagttg catcatcatc agacatattc atcatatctt catttttaga tttagaaatt
     8281 gtgacttcga cacctttttc aaaggcatgt acttgaatcc ataatggacc ttctacaaca
     8341 aaatcttctt cttcgttaat ttcatccatc attgaccaaa agaattcttc gccacgtttg
     8401 cgatttgtcc ataaatcttc acgactaaat ccacgggcct cgatatcgct atatgttata
     8461 aacaatttta cagttgtatc atctactcgt tctattctca tatcatctca ctccttacag
     8521 tcgatgaata gtatccacat tgtattaaag catcaaacaa aatacaattt atttgtttga
     8581 tttctttctc taaaatattt tggtaactta taataacata ttcgttgtag caaaactatt
     8641 taaatgattt atacagttaa agcattaaag cactttctta atttaatttt accttatcat
     8701 agcaaatgtt tcatccaaaa cactctaata tagagctata ttaacaattg cacatttgat
     8761 ttaaaagata ctaagcaatt attgtaaaat taaaaccaaa aaagcaacac tatagaaata
     8821 gtgttgcttg acatatcttt tagtaaagta tgacacatag ttaaatggtt attagtcaac
     8881 catacgttgt gcttcttgta attggaacgt acgaactttt ctaggtaaga aacgtcgaat
     8941 ctcgtcctca ttataaccaa cttgtagtcg tttattatct aaaataattg gacgacgtaa
     9001 taagccagga ttatcttgaa tgattgaata taagtcttgt aatggtagtg aatcaatatc
     9061 aacatttaat ttttggtatg ttttagaacg tgtagaaatg atttcatcag taccgtcttc
     9121 agtcattttt aatatttgct taatttcatc aattgttaaa tgttcagaaa aaatattacg
     9181 ctccgtatac ggaatgtcat gttcttgtaa ccatgctttc gctttacggc aagatgtgca
     9241 acttggtgaa gtaaataatg ttaccataca tctcactctc ctatttgaat gaataaaatt
     9301 cattgcttaa aatttagtta tagatcaaga aaaaacattt ttttctaaaa ttcttaatcg
     9361 ttactattta ttataactat ctaacattaa aattaaatga gaaaaaccta atttttcaga
     9421 taagtttcta cacttataaa aaagattatt aatccctttg ttagtagtaa gttatacgta
     9481 tattctaaca catcttacat ttttaagaaa tactgttata atgataatta ttaaaatatt
     9541 actaagaaag taggcattta aatggagaca ttattttcag gcatccaacc tagtggaatt
     9601 cctactattg gaaattatat tggcgcacta aaacaatttg ttgatgtgca aaatgactat
     9661 gattgttatt tctgtatcgt agatcaacat gcaattacaa tgccacaaga tcgtttaaaa
     9721 ttacgtaaac agaccagaca attagcagcg atttatttag cttctggtat agatccagac
     9781 aaagcaacat tgttcataca atctgaagtc cctgcacacg tacaagcagg atggatgtta
     9841 actacgattg cttctgttgg agaattagag cgtatgacgc aatacaaaga taaagctcag
     9901 aaagcagttg aaggtatacc tgctggtcta ttaacatatc cacctttaat ggcagctgat
     9961 attgttcttt acaatactaa tatcgttcca gttggagatg accaaaagca gcatatcgaa
    10021 ttgactcgta accttgtaga tagatttaat agtcgctata atgatgtgct tgtgaaacct
    10081 gaaattcgta tgcctaaagt tggtggacgt gtcatgagtt tacaagaccc aacaagaaaa
    10141 atgagtaaga gtgatgataa tgctaaaaac ttcatttcat tattagacga gccgaatgtt
    10201 gcagctaaaa aaattaaaag cgcagtaact gattcagatg gtattattaa atttgatcgt
    10261 gacaacaagc caggtataac aaatttaatt tcaatatacg ctggattaac agacatgcca
    10321 attaaagata ttgaggcaaa atatgagggc gaaggttatg gtaaatttaa aggtgacctt
    10381 gctgaaatag ttaaagcatt tttagtagaa ttccaagaaa aatacgaaag tttctataac
    10441 tcagataaac ttgatgatat tttagatcaa ggtagagata aagcacacaa agtttcattt
    10501 aaaactgtca aaaaaatgga aaaagcgatg ggtttaggac gtaagagata aaataaagag
    10561 gtactagaat taactagtgc ctcttttatt atttaacgtc tacctctagg attaaaagca
    10621 tctttaatcc cttcccctac aaagtttata cttaatatag ttaatgtaat cattatagca
    10681 ggtggcatcc atatccacgg tttaccactt ataacatccc cttcttgagc atcactcaac
    10741 atattacccc aagatggtat tgctttacta attccgaatc ctaaaaagct caatcctgat
    10801 tccactacaa tcataccggc aaataacaat gttgcttgta cgataactac acttaatata
    10861 ttcggcaaaa gatgtttcaa aataatttta tatgtgggtg taccaattga ttttgctgcc
    10921 aaaaagtatt cattttcttt ttcttgaagt actttaccac gaacaagtct tgcaatacct
    10981 ccccaactta atacgataat aactagaaca agaattatgg cagatccata aggattttta
    11041 attttatctc caagtgcagc atttaataca attgcaaata ttaaaaatgg aaataacata
    11101 acaaattcgg ttattctcat taataatgta tcaacaatac ctccaaaata accagaaatc
    11161 actccaactg taattccaat caaaagcatt cctattgtag atgtaattcc aatggataat
    11221 gaaatacgac ctgcatataa caaacgacta aagttatccc taccaccaga gtctgtacca
    11281 agaatgtttt gtgctgtcat ttcacctttt atatttaata aatcttgttg gttcactgga
    11341 aaaggagcta ttaatggcgc tataattgat aca
//
LOCUS       DAKTGS010000093        11228 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.093, whole genome shotgun sequence.
ACCESSION   DAKTGS010000093 DAKTGS010000000
VERSION     DAKTGS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.093"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            76..1029
                     /locus_tag="O0286_001948"
     CDS             76..1029
                     /locus_tag="O0286_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170319.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1192..1395)
                     /locus_tag="O0286_001949"
     CDS             complement(1192..1395)
                     /locus_tag="O0286_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0170320.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1694..2182
                     /locus_tag="O0286_001950"
     CDS             1694..2182
                     /locus_tag="O0286_001950"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0170321.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2175..3110
                     /locus_tag="O0286_001951"
     CDS             2175..3110
                     /locus_tag="O0286_001951"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCY0170322.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3107..4414
                     /gene="rsmB"
                     /locus_tag="O0286_001952"
     CDS             3107..4414
                     /gene="rsmB"
                     /locus_tag="O0286_001952"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCY0170323.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4417..5511
                     /gene="rlmN"
                     /locus_tag="O0286_001953"
     CDS             4417..5511
                     /gene="rlmN"
                     /locus_tag="O0286_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCY0170324.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5518..6261
                     /locus_tag="O0286_001954"
     CDS             5518..6261
                     /locus_tag="O0286_001954"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCY0170325.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6258..8252
                     /gene="pknB"
                     /locus_tag="O0286_001955"
     CDS             6258..8252
                     /gene="pknB"
                     /locus_tag="O0286_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCY0170326.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8480..9355
                     /gene="rsgA"
                     /locus_tag="O0286_001956"
     CDS             8480..9355
                     /gene="rsgA"
                     /locus_tag="O0286_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCY0170327.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9356..10000
                     /locus_tag="O0286_001957"
     CDS             9356..10000
                     /locus_tag="O0286_001957"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCY0170328.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            10007..10648
                     /locus_tag="O0286_001958"
     CDS             10007..10648
                     /locus_tag="O0286_001958"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCY0170329.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(11029..11217)
                     /locus_tag="O0286_001959"
     CDS             complement(11029..11217)
                     /locus_tag="O0286_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCY0170330.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
BASE COUNT         4171 a         1548 c         2102 g         3407 t
ORIGIN      
        1 atcaatataa ataagttttt taaatttatg tatgtttatt tgattcaaac aaaataactt
       61 aagaggagaa agtttatgaa aaagacactg ggatgtttac ttttaattat gcttttagtc
      121 gtagcaggtt gttcttttgg tgggaatcat aaattatcat caaagaaatc agaagaatca
      181 aaacaagaaa ctgtaaaaaa agaatcggaa gaagagaaag atccagattt agagaaatat
      241 gaagaaatag agaagaaaat gaaaggaatt aaagatgcgc catctcttga taagttggat
      301 ccattaatga cagaaaagtc gtttacgaat agtaaaggga ttcaaggatg gaaagattac
      361 aaagaattaa tgggtaaagt ggaacttgca gattatagat ttactaaaga ttcaaaagga
      421 tcttcaataa aagatgttga tgcattcttt aaaggtaaga aaggtataaa aaggaaagtg
      481 attgaaacac acgatgatgt aaaacaagtt gattattggt atgtagatcc agatggaaag
      541 aaaattggca attcaaacac acctgttttt tacgcagaaa ttatgacaaa atataaagat
      601 ggaaagttag tttatgcatc agtcgaacca ggatcttacg taatacataa agatgatgca
      661 attaaatatg acgattattc taagttaaaa aaattaagtc agctaactaa acttgatcat
      721 ccaaaaccag ttccatatag cgtagctcaa atcaaatctt tcggagtacc tttaacaagc
      781 gtttcattta tgacacatgg atcaaaggat actaaagatg aagtgttgcc ggcattggcc
      841 tatttcactt tttcaccaaa aaattatgaa gacaagtcta atccagatcc aaaagtttta
      901 aatttagtac atatggattt cttaaatgca tctagtgatt ttggtaacgc acattttgtt
      961 gttttaagta aatatattaa agagtatgaa tcaaactatg aaacagcgtc agatgattct
     1021 ttaaaatagt atttactgtg tgaaaaataa atagtgtact acattaaata atcgcaataa
     1081 taatcccgat aaacaatcag cattactgct tatcacatag agttcgtaat aactataact
     1141 ctatgattcg caaataataa atgattgtca tcgggattta tttttatcaa tttataaagt
     1201 gacattacct tgttcatcag caggtttgaa aacagtaatc actgcactaa taattgctaa
     1261 aatgtgtggg atacctgtcc aacagaatat taagtgtaga ataccttgca tattcttgcc
     1321 ggcataaaat ttatgaatac caaaactacc taagaacaat gctaataaaa tataaataac
     1381 tttgtttact tgcatttctt tccctccagt tgaattgctt ataatgacat tagcttctct
     1441 ttttattata cccactttta gttcaaacat tctagtttaa gcattcccaa tcatctaaat
     1501 ttcagttatt caatccttac aataaattta ggattacatt tcagttgcat tgtattattt
     1561 tacgtgtgaa atatacgtaa tgaatcacat gacaatcttc aaattgaaaa atatacattc
     1621 tatgatgtaa ggtcgcattt ttaatatatt tacgttataa tagtttgatg aattttattg
     1681 aggagtaaga gttatggcga ttaaaaagtt agtaccagca tcgcatccta ttttaacgaa
     1741 aaaagcgcaa gcagttaaaa catttgatga ttcgttaaaa agattattac aagatttaga
     1801 agatacaatg tatgcacaag aagctgctgg cttatgtgca cctcaaatta atcagtcatt
     1861 gcaagtggca attattgata tggaaatgga aggattatta caacttgtta atccgaaaat
     1921 tattagtcaa tcaaatgaaa caataacaga cttagaaggt tcaattacat tgccagatgt
     1981 ttacggcgaa gtgacaagaa gtaaaatgat agttgtcgaa agttatgacg tcaatgggaa
     2041 caaagttgaa ctaactgcac atgaagatgt agcaagaatg attttgcata ttatagatca
     2101 aatgaacggt atccctttta cagaacgtgc ggaccgtatt ttaacagata aagaagtgga
     2161 ggcatatttt ataaatgact aaaataatat ttatgggtac accagacttt tcaacaactg
     2221 ttttagaaat gcttattgca gaacatgatg tcattgcagt cgtaacgcaa ccagatcgac
     2281 ctgttggacg taaacgtgtt atgacaccac caccagttaa aaaagttgca atgaaatatg
     2341 atttacctgt atatcaacct gaaaaattaa gtggatcaga agaattagaa caattgcttc
     2401 aattagatgt agatttaatt gtaactgctg cttttggaca attattacct gaatcattgt
     2461 tggcattacc aaatcttggg gcaattaatg tacatgcatc attgttaccg aagtatagag
     2521 gtggtgcacc aattcatcag gcaattatcg atggtgaaca agaaaccggc ataacaatta
     2581 tgtatatggt taaaaaatta gatgcgggta atattatttc gcaacaagca attaaaatag
     2641 aagaaaatga taatgtcggt acgatgcatg ataaattaag tgtattaggg gcagatttat
     2701 taaaagaaac tttaccatct attatagagg gcacaaatga aagcgtacct caagatgata
     2761 cgcaagcaac atttgcttcc aatattcgac gcgaagatga gcgaattagc tggaataaac
     2821 caggaagaca agtgtttaat caaattcgtg gattatcacc atggccagtt gcttatacaa
     2881 ctatggatga cactaacttg aaaatatacg atgctgaact cgttgagact aataagataa
     2941 acgagcctgg aaccattata gaaacgacta aaaaagccat tattgttgct acaaatgata
     3001 atgaagctgt tgcaattaaa gatatgcaat tagctgggaa aaagagaatg ttagctgcca
     3061 attatttaag tggtgcgcaa aacacactag tagggaagaa acttatatga tagaaaacgt
     3121 gagaagtctt gcttttgaca cgattcaaga tatattaaat gaaggtgcgt atagtaactt
     3181 gcgtatcaat gaagtgttgt cagaaaatga attaaatgca atggataagg ctttatttac
     3241 agaaattgtc tacggaaccg ttaaaagaaa atatacgtta gatttttatt taaagccttt
     3301 tgtgaaaaca aagattaagg catgggttag gcaattatta tggatgagta tttatcaata
     3361 tgtttattta gataaagttc caaatcatgc cattattaat gaagcagttg aaatagcaaa
     3421 agaacgcggt ggctatcata atggtaatgt cgtaaatggt attttacgta caatgatgcg
     3481 tagtgactta cctgatttta atgaaattgc agatcctaaa aaaagaatgg caatcgagta
     3541 tagtatgccg aagtggatta tagatcattg ggcaacacat tatggtctcg aagaaactga
     3601 aacaatttta cagtcatttt tagaaacgac atcaacaact gtgcgtgcca acctgacgcg
     3661 agcatcatta gatgatatta ttgaaaagtt gcaagacgaa ggttatgacg ttgaaaaaga
     3721 tcatgactta ccttattgtc tccatatagg aggacaacca attattcatt ctcgttcatt
     3781 taaagatgga ttcgtttcaa ttcaagataa aagctcaatg tttgttgcac acattatgaa
     3841 tgtagaccga catgatcacg tattagatgc atgtagtgca cctggcggta aagcttgtca
     3901 cattgctgaa gttttaatgc cagaagggca agttgacgct tcagatatac atgatcacaa
     3961 aatagactta attaatttta atataaaaaa attacgatta acaaatatta aagcttttca
     4021 acatgatgcg acaaaacctt atgataaaac atacgataag atacttgttg atgcaccatg
     4081 tagcggatta ggtgtaatga gacataagcc ggagattaag tatactcaaa gcaaacaaca
     4141 tattgagtca ctagttgaat tacagcttga aatattggaa aatgtaaaaa acaatgtaaa
     4201 aataggtggc gaaatcatct attcaacatg tacaattgag caactagaaa atgaaaacgt
     4261 gatttatacg tttttgaaaa ataataaaaa cttcgaattt gaaccgtttc aacatccgat
     4321 aactggagag ttggtcaaaa cgttacaaat catgccgcaa gactttaatt cagatggatt
     4381 ctttatcact aagataaaaa gaaaggacaa ttaggaatga taactgctga aaagaaaaag
     4441 aagaataaat ttcttccaaa ttttgacaag caatcaatat attcattgcg atttgacgaa
     4501 atgcaaaact ggctcgttga acaagggcaa caaaaatttc gagcgaaaca gatttttgaa
     4561 tggttatatc aaaaaagagt agattcgatt gatgaaatga cgaacttatc gaaagactta
     4621 cgacagcttt taaaagataa ctttactgtt acaactttaa caactgtagt aaaacaagaa
     4681 agtaaagacg gtacaattaa attcttattt gaattacaag atggctatac aattgaaact
     4741 gttttaatga gacatgatta tggaaattca gtatgtgtaa cgacacaagt aggttgtcgc
     4801 atcggatgta cgttttgtgc ttctacactt ggcggcttaa aaagaaacct tgaagctggc
     4861 gaaattgttt cacaagtttt aacagttcaa aaagcccttg atgctacaga agagcgcgta
     4921 tctcaaattg tcataatggg tatcggtgaa ccatttgaaa attatgatga aatgatggac
     4981 tttttaagaa tcgtcaatga tgataacagt ttaaatattg gtgcacgtca cattacagta
     5041 tcaacatcag gtatcattcc tagaatatat gactttgcgg atgaagatat tcaaattaat
     5101 tttgctgtaa gcttacacgc cgcaaaagat gaagtgcgat cacgcttgat gccaattaac
     5161 cgtgcatata atgttgagaa gttaatcgaa gcaattcaat attatcaaga aaaaacaaat
     5221 cgtcgtgtta cttttgaata tggtctgttt ggtggtgtga atgaccaact agaacatgca
     5281 agagaattag cacatttaat aaaaggctta aactgccatg ttaacttaat tcctgtcaac
     5341 catgttccag aaagaaatta tgtgaaaacg gctaaaaatg atatctttaa atttgaaaaa
     5401 gaattaaaga gactaggaat taatgccaca atacgtcgtg aacaaggttc ggatattgac
     5461 gcagcttgtg gtcaattaag agcaaaggaa cgacaagtag aaacgaggta aagacaaatg
     5521 ctagaggcac aattttttac tgatactgga caacatagag ataagaatga agatgcgggt
     5581 ggtatttttt ataatcaaac taatcaacaa cttttagttc tgtgtgatgg tatgggtggc
     5641 cataaagcag gagaagttgc aagtaaattt gttacagatg agttgaaatc ccgttttgaa
     5701 gcggaaaatc ttatagaaca acatcaagct gaaaattggt tgcgtaataa tataaaagat
     5761 ataaattttc agttatatca ctatgcacaa gaaaatgcag aatataaagg tatgggtaca
     5821 acatgtgttt gtgcacttgt ttttgaaaaa tcagttgtga tagcaaatgt cggtgattct
     5881 agagcctatg ttattaatag tcgacaaatt gaacaaatta ctagtgatca ctcatttgtt
     5941 aatcatcttg ttttaacggg tcaaattacg ccggaagaag catttacaca tccacaacgt
     6001 aatattatta cgaaggtgat gggcacagat aaacgtgtga gtccagattt gtttattaag
     6061 cgattaaatt tttatgatta tttattatta aattcagatg gattaactga ttatgttaaa
     6121 gacaatgaaa ttaagcgttt gttagtaaaa gaaggtacaa tagaagatca tggtgatcaa
     6181 ttaatgcaat tggcattaga taaccattcg aaagataacg ttactttcat actcgcggct
     6241 attgaaggtg ataaagtatg ataggtaaaa taataaatga acgatataaa attgtagata
     6301 agcttggcgg cggtggcatg agtaccgttt atcttgctga agatacgata cttaacatta
     6361 aagttgcaat taaggcgatt tttataccac ctagagaaaa agaagaaaca ttaaaacgtt
     6421 ttgaacgaga agtacataac tcatcacagc tatcacatca aaatatagta agtatgatcg
     6481 atgttgatga agaagatgac tgttactact tagtaatgga atatatcgaa ggtccgactt
     6541 tgtctgagta tattgaaagt catgggccat taagtgttga cacagcgatt aattttacga
     6601 atcaaatatt ggatggcatt aaacatgcgc atgatatgcg tattgtacat agagatatta
     6661 agccacaaaa tatattaatt gacagcaata aaacgttgaa aatatttgat tttggaattg
     6721 ctaaagcttt aagtgagacg tctttaactc agactaatca tgtgttaggt actgtgcagt
     6781 acttttcgcc agaacaagca aaaggtgagg caacggatga atgtacagat atttattcta
     6841 taggtattgt gttatatgaa atgcttgttg gtgaaccacc ctttaatgga gaaactgcag
     6901 ttagcattgc gattaaacat attcaggatt ctgtgccaaa tgtgacaaca gatgtacgta
     6961 aggatattcc gcaatcttta agtaatgtca ttttacgcgc tacagaaaaa gacaaagcga
     7021 atcgttacaa aacaattcaa gaaatgaaag atgatttgag tagtgtttta catgaaaatc
     7081 gagcgaatga agatgtctat gaactcgata aaatgaaaac gatagcggta cctttgaaaa
     7141 aagaagatct agcaaagcat attagtgaac ataagtcgaa tcaacctaaa cgtgaaacga
     7201 cgcaagtacc tattgtaaat gggcctgctc atcatcagca attccaaaag ccagaaggta
     7261 cggtgtacga accaaaacct aaaaagaaat caacacgaaa gattgtgctc ttatcactaa
     7321 tcttttcgtt gttaatgatt gcacttgttt cttttgtggc aatggcaatg tttggtaata
     7381 aatacgaaga gacacctgat gtaatcggga aatctgtaaa agaagcagag caaatattca
     7441 ataaaaacaa cctgaaattg ggtaaaattt ctagaagtta tagtgataaa tatcctgaaa
     7501 atgaaattat taagacaact cctaatactg gtgaacgtgt tgaacgtggt gacagtgttg
     7561 atgttgttat atcaaagggc cctgaaaagg ttaaaatgcc aaatgtcatt ggtttaccta
     7621 aggaggaagc cttgcagaaa ttaaaatcgt taggtcttaa agatgttacg attgaaaaag
     7681 tatataataa tcaagcgcca aaaggataca ttgcaaatca aagtgtaacc gcaaatactg
     7741 aaatcgctat tcatgattct aatattaaac tatatgaatc tttaggcatt aagcaagttt
     7801 atgtagaaga ctttgaacat aaatccttta gcaaagctaa aaaagcctta gaagaaaaag
     7861 ggtttaaagt tgaaagtaag gaagagtata gtgacgatat tgatgagggt gatgtgattt
     7921 ctcaatctcc taaaggaaaa tcagtagatg aggggtcaac gatttcattt gttgtttcta
     7981 aaggtaaaaa aagtgactca tcagatgtca aaacgacaac tgaatcggta gatgtaccat
     8041 acactggtaa aaatgataag tcacaaaaag ttaaagttta tattaaagat aaagataatg
     8101 acggttcaac tgaaaaaggt agtttcgata ttactagtga tcaacgtata gacattcctt
     8161 taagaattga aaaaggaaaa acagcaagtt atattgttaa agttgacggt aaaactgtag
     8221 ctgaaaaaga agtcagctat gatgatgtat aaatataatt gaagtaaatg taccgaggtt
     8281 tctatttgga agtctcggta tttttatgtt gagattgcgg tagttttaaa atgcttcatg
     8341 tcatcatata caggtgagca ttgtctatca attctgtatc actctcacaa tatttatatc
     8401 atttgaaatg atcatttata tattaatttt ccagtctttg atataataaa tgcagtaaaa
     8461 ttaatcgaga ggtgccattt tgaagacagg tcgaatagtg aaatcaatta gtggggtata
     8521 tcaagtagac gttaatggcg aacgtttcaa tacaaaacca cgaggattat ttagaaagaa
     8581 aaaattttca ccggtagttg gtgatatagt ggaatttgaa gttcaaaaca ttaacgaagg
     8641 ttatattcat caagtgtttg agcgggaaaa tgagttgaaa agaccacctg taagtaatat
     8701 agatacacta gtaattgtaa tgagtgctgt cgagccaaat ttttcaacgc aattattaga
     8761 tcgattttta gttattgcac attcgtatca gttaaatgcg agaattttgg tgactaaaaa
     8821 agataaaaca ccaattgaaa agcagttcga aataaatgag ttgttgaaaa tatatgaaaa
     8881 tattggctat gagactgaat ttattggaaa tgatgatgat cgaaaaaaaa ttgtagaagc
     8941 ttggccagct ggacttatag tacttagtgg tcaatcaggt gtcggtaagt ccactttctt
     9001 aaatcattat cgtccagaac ttaatcttga gacaaatgat atatcaaaat cattaaatcg
     9061 aggaaagcat actacaagac atgtcgaact attcgaacgt caaaacggtt atattgcaga
     9121 cacacctgga ttcagtgctt tagattttga tcatatagat aaagatgaaa taaaagatta
     9181 ttttcttgaa ttaaatcgat atggtgaaac atgtaagttt aggaattgta atcatatcaa
     9241 agaacctaat tgtaatgtta agcatcaatt agagataggg aatattgcgc aatttagata
     9301 cgaccattat ttacaactat ttaatgaaat ttcaaataga aaggttagat attaaatgac
     9361 aaaactatat ccatcattat tatctgttga ttttttggat ttacaacatg aattaaaacg
     9421 acttgaagaa gcaggcgtcg acggagttca ttttgatgtt atggatggtc aatttgtgcc
     9481 taatatatct attggtttac caatattaga tgcagtaaga aaaggcacaa cattacctat
     9541 agacgtacat ttgatgattg aaaatccaga aaagtatatt gcatcatttg cagaacatgg
     9601 tgccgatatg atttcaattc atgtcgaatc aacgcctcat attcatcgtg ctattcaaat
     9661 gattaaacat ttagataaaa aagctggtgt agtaattaat cctggtacac caatatcaca
     9721 aattgaacct attttagaca ttgttgatta tgtactagtg atgacagtta acccagggtt
     9781 tggtggtcaa tcatttattg atcaatgcgt agaaaaaata gcgggtctta atgctattaa
     9841 aatggaacgt caattaaact ttgatattga agttgatgga ggcgtaaata ccgatacagc
     9901 gaaagtttgt gttgaaaatg gtgctacaat gctagtaaca ggttcatttt tctttaaaca
     9961 agaggattat aaaaaagtca cacaagcatt gaaaggttga gtgtatatgc atataaattt
    10021 attatgttct gatcgacact tgccgcaaga tatttgggcc aaaagtaatg aaggtaaatg
    10081 gggcggcgtt gatagaggtg ctttgatttt attgaagcat caaattatcc cttttttctc
    10141 agtgggagac tttgattcag tcagtaaaga agaacgccaa cttctaacag aacagttaca
    10201 aatcaaacca gttcaagctg aaaaagctga tacggattta gctttagcgg ttgataaagc
    10261 tgttgcactt ggatttgata gtattacaat ttatggtgca acaggcggac gattagatca
    10321 cttttttggg gcaattcagt tattattgaa aaaagcatat tataaacatg atgttcatat
    10381 agaagttatc gatcaacaaa ataaaattga attattgcct aaaggtcaac atacagttga
    10441 aaaagataag agttatccgt acatttcatt tataccgatg actgatgatg tagaactttc
    10501 tctagcaggt tttaaatata atttagctag acaaatgctt aatataggtt ctactttaac
    10561 tatttcaaat gaaattgagt ctttgcaagc gaaagtaact gtacatgatg ggttgatttt
    10621 gcaaattaga agtacagatt taaattaagt atattcaaat ttgtgaggat ttttaataaa
    10681 gtgcatgagt tatttaataa agtgacgttt atagatagaa tttataacat aataatagat
    10741 gaatcacgct gtaattaatg aagattatgg cgtgatttat tattgaaagg aaaattgtag
    10801 gtcctagtac attgatttat gtgccaaaat cgatattgaa cccataagtc caatttgaca
    10861 gagaaccatt attaaacgta ttaaccaaat tatattgata gttcgaattt cactatgaac
    10921 cattatgtat tgtttggtaa caaaaaaaga actaagaggt tgagctctta gttcttaagt
    10981 aaatgtgtcc tccacataag gtcacatttt ttatgtgcaa tatatttatt aaactctagt
    11041 tactttacca gattttaaag cacgtgcaga aacccaaact tttttaggtt taccgtcaac
    11101 taggattcta actttttgaa ggttagcgtt ccatctacgt ttagtagagt ttaaagcgtg
    11161 tgaacgtctg ttaccagtcg aagctttacg acctgttacg aaacattgtt tacccatatg
    11221 agtacctc
//
LOCUS       DAKTGS010000094        11223 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.094, whole genome shotgun sequence.
ACCESSION   DAKTGS010000094 DAKTGS010000000
VERSION     DAKTGS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.094"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            158..1846
                     /gene="ilvD"
                     /locus_tag="O0286_001960"
     CDS             158..1846
                     /gene="ilvD"
                     /locus_tag="O0286_001960"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCY0170331.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1874..3643
                     /gene="ilvB"
                     /locus_tag="O0286_001961"
     CDS             1874..3643
                     /gene="ilvB"
                     /locus_tag="O0286_001961"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCY0170332.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3643..3897
                     /locus_tag="O0286_001962"
     CDS             3643..3897
                     /locus_tag="O0286_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0170333.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4034..5038
                     /gene="ilvC"
                     /locus_tag="O0286_001963"
     CDS             4034..5038
                     /gene="ilvC"
                     /locus_tag="O0286_001963"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCY0170334.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5068..6597
                     /locus_tag="O0286_001964"
     CDS             5068..6597
                     /locus_tag="O0286_001964"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0170335.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6600..7646
                     /gene="leuB"
                     /locus_tag="O0286_001965"
     CDS             6600..7646
                     /gene="leuB"
                     /locus_tag="O0286_001965"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCY0170336.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7660..9030
                     /gene="leuC"
                     /locus_tag="O0286_001966"
     CDS             7660..9030
                     /gene="leuC"
                     /locus_tag="O0286_001966"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCY0170337.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9031..9603
                     /gene="leuD"
                     /locus_tag="O0286_001967"
     CDS             9031..9603
                     /gene="leuD"
                     /locus_tag="O0286_001967"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCY0170338.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9618..10886
                     /gene="ilvA"
                     /locus_tag="O0286_001968"
     CDS             9618..10886
                     /gene="ilvA"
                     /locus_tag="O0286_001968"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCY0170339.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(11096..11210)
                     /gene="rrf"
                     /locus_tag="O0286_001969"
     rRNA            complement(11096..11210)
                     /gene="rrf"
                     /locus_tag="O0286_001969"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3768 a         1864 c         2186 g         3405 t
ORIGIN      
        1 aaatatttac taaacgcttt aagtcctatt tctgtttgaa tgggacttgt aaacgtccca
       61 ataatattgg gacgtttttt tatgttttat ctttcaatta cttattttta ttactataaa
      121 acatgattaa tcattaaaat ttacggggga atttactatg cgaagcgaca tgatcaaaaa
      181 aggagatcac caagcaccag caagaagtct tttacatgcc acgggcgcgc taaaaagtcc
      241 aactgatatg aacaaaccat ttgtagctat ttgtaactct tatattgata ttgttcctgg
      301 acatgttcac ttgagagagc ttgcagatat agctaaagaa gcaattagag aagccggtgc
      361 cattccattt gaattcaata caattggtgt tgatgatgga atagctatgg gacatatcgg
      421 aatgcgatat tctctaccat cacgtgaaat tattgcagat gcagctgaaa ctgtaattaa
      481 cgctcattgg tttgacggcg tattttacat tcctaattgt gacaagatta cacccggtat
      541 gattttagca gccatgagga caaacgtacc agctatcttt tgctctggtg gaccaatgaa
      601 agctggctta tctgcacatg gaaaagcatt aacactttca tcaatgtttg aagcagtcgg
      661 cgcatttaaa gaaggatcga tttctaaaga agaattttta gatatggaac aaaatgcctg
      721 ccctacttgt ggttcatgtg ctgggatgtt tactgcaaat tcaatgaact gtttgatgga
      781 agttttaggt ctaacattac catacaacgg tactgcactt gcagtcagtg atcagcgacg
      841 agaaatgatt cgccaagcag cttttaaatt agttgaaaat attaaaaatg atttaaaacc
      901 acgtgatatc gttactcgcg aagcaattga tgatgcattt gcacttgata tggctatggg
      961 tggttcaaca aacacggtac tgcatacgtt agccattgcc aatgaagctg gtattgatta
     1021 tgacttagag cgcattaatg ctattgccaa acgcacgcca tatttatcaa aaatagcacc
     1081 tagttcatcg tattcaatgc atgatgtgca tgaagctggt ggcgtcccag caattattaa
     1141 tgaattgatg aagaaagatg gcacgttaca cccagataga atcacagtta ctggcaaaac
     1201 gttacgtgaa aataacgaag gcaaagaaat taagaacttt gatgtcattc accctcttga
     1261 tgcaccatat gatgcacaag gcggtttatc tatcttattt ggtaatatcg cccctaaagg
     1321 cgcagttatt aaagttggcg gcgttgatcc atctatcaaa acatttactg ggaaagcaat
     1381 ttgtttcaat tcgcatgatg aagctgttga agcaatagac aatcgtaccg ttcgtgcagg
     1441 ccacgtcgtt gtcattagat atgaaggacc taaaggtgga ccaggtatgc ctgaaatgtt
     1501 agcacctact tcctctattg ttggtcgcgg cttaggtaaa gatgttgcat taattactga
     1561 tgggcgtttt tccggtgcca caagaggtat tgcagttggt catatttccc ctgaagctgc
     1621 atctggtgga ccaattgcct taattgaaga tggtgatgag attactattg atttaacaaa
     1681 tcgtacatta aacgtaaacc agcctgaaga tgttctagcg cgtcgccgag aatctttaac
     1741 accatttaaa gcgaaagtaa aaacaggtta tctagctcgt tatactgccc tagtaactag
     1801 cgcaaataca ggtggcgtca tgcaagtccc tgagaattta atttaattta tttttatatt
     1861 ggagatggtt aaaatgtcta aaactcaaca tgaagtaaac caaaatattg accctttaaa
     1921 aatggctgaa tcacttgaac ctgaacaact aaatgaaaaa actttaaatg atatgcgttc
     1981 aggatcagaa gtgctagtag aagctctact taaagaaaat gtggattatt tattcggtta
     2041 tcctggtggt gccgtactac ctttatatga cacgttttat gatggtaaaa tcaaacatat
     2101 tttagcaaga cacgaacaag gtgctgttca tgctgcagaa ggttatgcac gtgtatctgg
     2161 taaaactggc gtcgttgtag ttacaagcgg tccaggtgca actaatgtaa tgacaggtat
     2221 tacggatgca cattgcgact ctttacctct agttgtattc actggacaag ttgctacacc
     2281 aggcattggt aaagatgcat tccaagaagc ggatattcta tctatgactt caccaattac
     2341 aaaacaaaat tatcaagtga aacgtgttga agatatccct aaaatcgtac acgaagcttt
     2401 ccatgtagct aattctggac gcaaaggtcc tgtagtgatt gattttccaa aagatatggg
     2461 tgttttagct acaaatgtgg atttatgcga cgaaatcaat attccaggtt atgaagttgt
     2521 tacagaacca gaaaataaag acattgacac tttcatctca cttttaaaag aagcgaaaaa
     2581 gcctgtcgta ttagccggcg caggtattaa tcaatcaaaa tcaaatcaat tattaacaca
     2641 gtttgttaat aaacatcaaa tcccaacagt tactacttta ctaggtctag gtgctgtacc
     2701 ttatgaggat acactatttt taggtatggg aggaatgcat ggttcttatg ctagtaacat
     2761 ggcattaact gagtgtgatt tactcattaa tttaggtagc cgcttcgatg atagattagc
     2821 aagcaaacct gatgcctttg cacctaacgc caaaattgta catgtagata ttgatccttc
     2881 agaaatcaat aaagttattc atgtagattt aggtattatt gcagactgta aaagattttt
     2941 agaatgttta aatgataaaa atgttgagac tatagaacac agtgactggg ttaaacattg
     3001 tcaaaataat aagcagaaac acccatttaa acttggtgaa gaagatcaag tattttgtaa
     3061 gccacaacaa acaatcgaat atatcggcaa aattacaaat ggtgaagcaa ttgttactac
     3121 agacgtggga caacatcaaa tgtgggcagc tcaattttat ccatttaaaa atcacggaca
     3181 atgggttaca agcggtggtt taggaacaat gggattcggt attccttcgt caattggtgc
     3241 caaattagct aatcctgata aaacagtcgt atgtttcgtc ggtgacggtg gtttccaaat
     3301 gacaaaccaa gaaatggcac ttttacccga atatggttta gatgtcaaaa tcgtactaat
     3361 caataatgga acattaggta tggttaaaca atggcaagat aagttcttta atcaacgctt
     3421 ctcacactca gtatttaatg gtcaacctga ttttatgaaa atggcagaag catatggcgt
     3481 caaaggtttc ttaatcgata agccagaaca actggaagaa caattagatg cagcgtttgc
     3541 ttatcaagga ccagctttaa ttgaggttcg tatttcccct actgaagctg taaccccaat
     3601 ggttccgagt ggcaaatcaa atcatgaaat ggagggctta taatgacaag aattcttaaa
     3661 ttacaagttg cggatcaagt cagcacgcta aatcgaatta caagtgcttt tgttcgccta
     3721 caatataata tcgatacatt acatgttaca cattctgaac aacctgggat ttctaacatg
     3781 gaaattcaag tcgatattca agatgataca tcacttcata tattaattaa aaaattaaaa
     3841 caacaaatta atgttttaac ggttgaatgc tacgaccttg ttgataacga agcttaattt
     3901 taagacaaag gcaatgatgc gctaattagt tatagatata tcataggctg ctagttaaca
     3961 tctgccacta ttacaaagtt atatttcaga attttcgaaa cacaaaatat ttaattattt
     4021 ggaggaattt attatgacaa cagtttatta tgatcaagat gtaaaaacgg acgctttaca
     4081 aggcaaaaaa attgcagtag taggttatgg atcacaaggt cacgcgcatg cacaaaactt
     4141 aaaagacaat ggatatgatg tagtcatcgg cattcgccca ggtcgttctt ttgacaaagc
     4201 taaagaagat ggatttgatg tgttccctgt tgcagaagca gttaagcaag ctgatgtaat
     4261 tatggtgcta ttacctgatg aaattcaagg tgatgtatac aaaaacgaaa ttgaaccaaa
     4321 tttagaaaaa cataatgcgc ttgcatttgc tcatggcttt aacattcatt ttggtgttat
     4381 tcaaccacca gctgatgttg atgtattttt agtagctcct aaaggaccgg gtcatttagt
     4441 tagacgtaca tttgttgaag gttctgctgt accatcacta tttggtattc aacaaggcgc
     4501 ttcaggtcaa gcacgtaata ttgctttaag ttatgcaaaa ggtattggtg caactcgtgc
     4561 aggtgttatt gaaacaacat ttaaagaaga aactgagaca gatttatttg gtgaacaagc
     4621 agtactttgc ggtggtgtat cgaaattaat tcaaagtggc tttgaaacat tagtagaagc
     4681 gggttatcaa ccagaattag cttattttga agtattacat gaaatgaaat taatcgttga
     4741 tttgatgtat gaaggcggta tggaaaatgt acgttactca atttcaaata ctgctgaatt
     4801 tggtgactat gtttcaggac cacgtgttat cacaccagat gttaaagaaa atatgaaagc
     4861 tgtattaact gatatccaaa atggtaactt cagtaatcgc tttatcgaag acaataaaaa
     4921 tggattcaaa gaattttata aattacgcga agaacaacat ggtcatcaaa ttgaaaaagt
     4981 tggtcgtgaa ttacgcgaaa tgatgccttt tattaaatct aaaagcattg aaaaataaga
     5041 tagacctaca atgaggagtt gttaaatatg agtagtcata ttcaaatttt tgatacgaca
     5101 ctaagagacg gtgaacaaac accaggagtg aattttactt ttgatgaacg cttgcgtatt
     5161 gcattgcaat tagaaaaatg gggtgtagat gttattgaag ctggatttcc tgcttcaagt
     5221 acaggtagct ttaaatctgt tcaagcaatt gcacaaacat taacaacaac ggctgtatgt
     5281 ggtttagcta gatgtaaaaa atctgacatc gatgctgtat atgaagcaac aaaagatgca
     5341 gcgaagccgg tcgtgcatgt ttttatagca acatcaccta ttcatcttga acataaactt
     5401 aaaatgtctc aagaagacgt tttagcatct attaaagaac atgtcacata cgcgaaacaa
     5461 ttatttgacg ttgttcaatt ttcacctgaa gatgcaacgc gtactgaatt accattctta
     5521 gtgaaatgtg tacaaactgc cgttgacgct ggagctacag ttattaatat tcctgataca
     5581 gtcggctaca gttaccatga tgaatatgca catattttca aaaccttaac agaatctgta
     5641 acatcttcaa atgaaattat ttatagtgct cattgccatg acgatttagg aatggctgtt
     5701 tcaaatagtt tagctgcaat tgaaggcggt gcgagacgaa ttgaaggcac tgtaaatggt
     5761 attggtgaac gagcaggtaa tgcagcactt gaagaagtcg cgcttgcact atacgttcga
     5821 aatgatcatt atggtgctca aactgctctt aatctcgaag aaactaaaaa aacatcggat
     5881 ttaatttcaa gatatgcagg tattcgagtg cctagaaata aagcaattgt tggccaaaat
     5941 gcatttagtc atgaatcagg tattcaccaa gatggcgtat taaaacatcg tgaaacatat
     6001 gaaattatga cacctcaact tgttggtgta agcacgactg aacttccatt aggaaaatta
     6061 tctggtaaac acgccttctc agagaagtta aaagcattag gttatgacat tgataaagaa
     6121 gcgcaaatag atttatttaa acaattcaag gccattgcgg acaaaaagaa atctgtttca
     6181 gatagagata ttcatgcgat tattcaaggt tctgagcatg agcatcaagc actttataaa
     6241 ttggaaacac tgcaactaca atatgtctct agcggccttc aaagtgctgt tgttgttgtt
     6301 aaagataaag agggtcatat ttaccaggat tcaagtattg gtactggttc aatcgtagca
     6361 atttacaatg cagttgatcg tattttccag aaagaaacag aattaattga ttatcgtatt
     6421 aattctgtca ctgaaggtac tgatgcccaa gcagaagtac atgtaaattt attgattgaa
     6481 ggtaagactg tcaatggctt tggtattgat catgatattt tacaagcctc ttgtaaagca
     6541 tacgtagaag cacatgctaa atttgcagct gaaaatgttg agaaggtagg taattaatta
     6601 tgacttataa cattgttgcc ctacctggtg atggaatcgg tccagaaatt ttgaacggat
     6661 ctctatcatt gcttgaaatt ataagtaata aatataactt taattatcaa atagagcacc
     6721 acgaatttgg tggtgcctct attgatacat tcggcgagcc tttaactgag aaaaccttaa
     6781 atgcgtgtaa aagagcagat gctattttac tgggtgcaat cggtggacct aaatggacag
     6841 atcctaacaa tcgaccagaa caaggattat taaaattgcg taaatcctta aatttatttg
     6901 taaatatacg ccccactacc gttgtcaaag gcgctagttc tttatcacct ttaaaggaag
     6961 aacgcgttga aggcacagat ttagttatag tccgtgaatt gacaagtggt atttattttg
     7021 gagaacctag acattttaat aatcacgagg ccttagattc tcttacttat acaagagaag
     7081 aaatagaacg cattgttcac gtagcattta aattggccgc ttcaagacga ggaaaactaa
     7141 catcagttga taaagaaaat gtattagctt ctagtaaatt gtggcgcaaa gtcgtaaatg
     7201 aagtaagtca attatatcca gaagtaacag taaatcactt acttgttgat gcttgtagta
     7261 tgcatttaat cacaaatcca aaacaatttg acgtcatcgt atgtgaaaac ttatttggcg
     7321 atattttaag tgatgaagct tcagtgattc ctggttcact tggtttatca ccttctgcta
     7381 gttttagtaa cgatggtcca agattgtatg agcctattca tggatcagca ccagatattg
     7441 caggtaaaaa cgttgccaat ccatttggaa tgattctatc tttagcgatg tgtttacgtg
     7501 aaagcttaaa tcaaccagat gctgcagatg aattagaaca acatatttat agcatgattg
     7561 aacatgggca aacgacagca gatttaggcg gcaaattgaa tactactgat attttcgaaa
     7621 ttctatctca aaaattgaat cactaagggg gagatgtaaa tgggtcaaac attatttgac
     7681 aaggtgtgga acagacatgt gttatacggg aaattgggcg aaccgcaact attatacatt
     7741 gatttacacc ttatacatga agttacttct cctcaagcat ttgaaggact taggcttcaa
     7801 aacagaaaat taagacgccc agatttaaca tttgcaacac tcgatcacaa tgttcctact
     7861 attgatatat tcaatattaa agatgaaatt gcaaacaaac aaatcacaac attacaaaaa
     7921 aacgccatag attttggggt gcatattttt gatatgggtt ctgatgaaca aggtattgtt
     7981 cacatggtag gacctgagac aggacttaca cagcctggca agacaatcgt ttgtggtgac
     8041 tctcacacag caacacatgg agcatttggt gctattgcat ttggaattgg aacaagtgaa
     8101 gttgaacatg ttttcgcaac tcaaacgcta tggcaaacaa aacccaaaaa cttaaaaatc
     8161 gatattaatg gtaccttacc aacaggcgtc tatgctaagg acattattct gcatttaatt
     8221 aaaacgtatg gtgttgactt tggtacaggc tatgctttgg aatttactgg cgaaacaatt
     8281 aaaaaccttt caatggatgg tcgaatgact atttgtaaca tggctatcga aggtggtgcc
     8341 aaatacggca taatccaacc tgatgatata acatttgaat atgttaaagg gagaccattt
     8401 gccgataact tcgctaaatc agttgataag tggcgtgagc tatattctga tgacgacgcg
     8461 atatttgatc gtgtaattga acttgatgtt tcaacattag aaccacaagt gacatgggga
     8521 actaatcctg aaatgggtgt taatttcagt gaaccattcc ctgaaatcaa tgatatcaac
     8581 gatcaacgtg cgtatgatta tatggggtta gaaccaggtc aaaaagctga agacatcgac
     8641 ttagggtatg tttttctcgg ttcatgtaca aatgctagac tatcagattt gattgaagct
     8701 agtcatattg ttaaaggaaa taaagttcat ccaaatatta cagctattgt cgtaccaggt
     8761 tctcgtacag taaaaaaaga agcagaaaaa ttaggtctag atactatctt taaaaatgca
     8821 ggatttgaat ggcgtgaacc aggatgttca atgtgtttag gcatgaatcc tgaccaagta
     8881 cctgagggcg tacattgtgc atctacaagt aatcgaaact ttgaaggacg acaaggcaaa
     8941 ggtgcaagaa cacatttagt atcccctgct atggcagcag cagcagctat tcatggtaaa
     9001 tttgtggacg taagaaaggt ggttgtttaa atggcagcaa tcaaacctat tacaacatat
     9061 aaaggtaaaa tagtccctct cttcaacgac aatatcgata cagaccaaat cattcctaag
     9121 gtacacttaa agcgtatttc aaaaagtggc tttggtccat ttgcttttga tgaatggcgg
     9181 tacttacctg atggttcaga taatcctgat ttcaatccta acaaaccaca atataaaggg
     9241 gcttctattt taattactgg agataatttt ggatgtggtt caagtcgtga acatgctgct
     9301 tgggctctta aggactatgg ttttcatatt attattgcag gaagtttcag tgacatattt
     9361 tatatgaatt gcactaaaaa tgcgatgttg cctatcgttt tagaaaaaag tgcccgtgaa
     9421 catcttgcac aatatgttga aattgaggtc gatttaccaa atcaaactgt gtcatcacca
     9481 gacaagcgtt tccattttga aattgatgaa acttggaaga ataaacttgt aaatggctta
     9541 gatgacattg caatcaccct acaatatgaa tcattaatag aaaaatatga aaaatcactt
     9601 taagggagtt gaatattatg acagtcaaaa caacagtttc tacgaaagat atcgatgagg
     9661 catttttaag acttaaagat attgtcaaag aaacaccttt acaattagac cattacttat
     9721 ctcaaaagta tgattgtaaa gtctatttaa aacgagaaga tttacaatgg gtacgttctt
     9781 ttaaattaag aggtgcttac aacgctattt ctgttttatc agatgaagct aaaagtaaag
     9841 gtattacatg tgcaagtgca ggtaatcatg ctcaaggtgt tgcctataca gctaaaaaac
     9901 ttaatttaaa cgctgttatc tttatgccag tcactacacc tttacaaaag gtaaatcaag
     9961 taaagttctt tggaaatagt aacgttgaag ttgtactcac tggtgataca tttgatcact
    10021 gtttagctga agctttaact tatacaagtg aacatcaaat gaactttata gatccattca
    10081 ataatgttca tacaatttct ggacaaggta cgcttgctaa agaaatgcta gaacaagcaa
    10141 agtctgacaa tgttaacttt gattatctat ttgccgcaat tggtggtggc ggtttaattt
    10201 caggtattag tacttacttt aaaacctatt cacctaccac gaaaattata ggtgttgaac
    10261 cttcaggtgc aagtagtatg tatgaatctg ttgtggtaaa taatcaggta gtcacattgc
    10321 ctaatatcga taaatttgtg gacggtgcat ctgtagctag agttggcgat attacatttg
    10381 aaattgcaaa agaaaatgta gatgattacg ttcaagtaga tgaaggtgca gtttgttcta
    10441 cgattttaga tatgtattca aaacaagcaa ttgtagcaga acctgctggc gcattaagtg
    10501 taagtgcgct tgaaaactat aaagatcata ttaaaggtaa aacagtggtt tgtgtcatta
    10561 gtggtggtaa taatgatatt aatcgaatga aagaaattga agaacgttca ttactatacg
    10621 aagaaatgaa gcattacttt atcttaaatt tccctcaacg tccaggtgca ttgagagaat
    10681 ttgtaaatga cgtattagga cctcaagacg atattactaa atttgaatac ttaaaaaaat
    10741 cttctcaaaa tacaggtact gtcattattg gtattcaact taaagatcat gatgatttaa
    10801 tacaactcaa acaacgtgta aatcatttcg atccttccaa tatttatatt aatgaaaata
    10861 agatgttata ttcattgtta atttaacaca tagtaagaaa aacagtcata aattgatttc
    10921 taattgaaat catcttatga ctgcttttta ttatacttta catttctcgt ttcgtcagat
    10981 tcaaacgttt tcacttcgcc aagccatctt tctttgtgtt tgcttttatt ttgacgtttt
    11041 agacataaaa aaagagacct tgcggtctca atgcggctca tcgcatccac tttttgcctg
    11101 gcaacgttct actctagcgg aacgtaagtt cgactaccat cgacgctaag gagcttaact
    11161 tctgtgttcg gcatgggaac aggtgtgacc tccttgctat agtcaccaga catatgaatg
    11221 taa
//
LOCUS       DAKTGS010000095        10980 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.095, whole genome shotgun sequence.
ACCESSION   DAKTGS010000095 DAKTGS010000000
VERSION     DAKTGS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.095"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..217
                     /locus_tag="O0286_001970"
     CDS             <1..217
                     /locus_tag="O0286_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCY0170340.1"
                     /translation="LEIFSDEIIEFITLNNYVLIKTSPGFAQSISYYIDQLQMKEILG
                     IIGGNDTLMILTSSNEIAEFVCYQLFP"
     gene            486..1721
                     /gene="arcA"
                     /locus_tag="O0286_001971"
     CDS             486..1721
                     /gene="arcA"
                     /locus_tag="O0286_001971"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0170341.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1807..3228
                     /gene="arcD"
                     /locus_tag="O0286_001972"
     CDS             1807..3228
                     /gene="arcD"
                     /locus_tag="O0286_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0170342.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKAN
                     LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTL
                     FIFIIVLAIIGIVRLITGNISVF"
     gene            3270..3959
                     /locus_tag="O0286_001973"
     CDS             3270..3959
                     /locus_tag="O0286_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0170343.1"
                     /translation="MYEENIYIKNSEYEFDNNLKQLASYLNIPVSIVRPYKEDLTLYQ
                     YKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNYLRLSKDENIFPMNFIFNETPA
                     PYEICTALTDCKILTLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSA
                     KERIERILQILCLSIGDDNGEFYELKQIMTVQLISNLSGLNRKTTGEIIRELKIENII
                     YQDKRNWIIKK"
     gene            3997..4995
                     /gene="argF"
                     /locus_tag="O0286_001974"
     CDS             3997..4995
                     /gene="argF"
                     /locus_tag="O0286_001974"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0170344.1"
                     /translation="MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMK
                     GKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYD
                     GIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
                     VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDI
                     DEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPS
                     FHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            5015..5944
                     /gene="arcC"
                     /locus_tag="O0286_001975"
     CDS             5015..5944
                     /gene="arcC"
                     /locus_tag="O0286_001975"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0170345.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
     gene            complement(6044..7044)
                     /locus_tag="O0286_001976"
                     /pseudo
     CDS             complement(6044..7044)
                     /locus_tag="O0286_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7076..7245
                     /locus_tag="O0286_001977"
                     /pseudo
     CDS             7076..7245
                     /locus_tag="O0286_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            7852..8202
                     /locus_tag="O0286_001978"
     CDS             7852..8202
                     /locus_tag="O0286_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170346.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
     gene            8289..8600
                     /locus_tag="O0286_001979"
     CDS             8289..8600
                     /locus_tag="O0286_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170347.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            8616..9119
                     /locus_tag="O0286_001980"
     CDS             8616..9119
                     /locus_tag="O0286_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0170348.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEVMHPLNPKA
                     RQKWTLK"
     gene            9133..9357
                     /locus_tag="O0286_001981"
     CDS             9133..9357
                     /locus_tag="O0286_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170349.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYEEFINDDSITTVLNNDLQTIINHYLSK"
     gene            complement(9612..10739)
                     /locus_tag="O0286_001982"
     CDS             complement(9612..10739)
                     /locus_tag="O0286_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0170350.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
BASE COUNT         3903 a         1683 c         1811 g         3583 t
ORIGIN      
        1 attagaaata tttagtgatg agattattga atttataacg ctaaataact atgtcttaat
       61 aaaaacatct cctggctttg ctcaaagtat aagttattac atagatcaat tacaaatgaa
      121 agaaatatta ggaattattg gaggtaacga tactttgatg attttgactt cttcaaatga
      181 aatagcagaa tttgtttgtt atcaattatt cccttaaaaa ttaaatgatt atattaatag
      241 gaatgattac taatacaaaa aaatgagtaa aaaatctttt tgcttgaata agataaggtt
      301 ttaacaatga gatttttcaa attattcact attatatagt acaaaataca tattcataca
      361 aaattttgca ttgaataaat ctatatatgt gaaaaccctc cctaaacaat tgtgaattaa
      421 atcacacgat gttttttgaa agcgttttat aatgtgaatg taataaagca aaggaggaat
      481 ttgaaatggt acaaggaccc attcaagtaa atagtgaaat aggcaaattg aaaactgtgt
      541 tgttaaaaag accaggaaaa gaattagaaa atttagtacc tgatcattta agtggtttat
      601 tattcgatga tattccctac ttaaaagttg cacaagaaga gcatgacaaa tttgctcaaa
      661 ctttgagaga tgaaggaatc gaagtagttt atttagaaaa acttgcagca gaatctatta
      721 ctgagccaga agtacgcgag aacttcataa acgacatatt aacagaatct aaaaagacaa
      781 tattaggtca tgaaactgaa attaaagaat tcttttcaaa gttatctgac caagaacttg
      841 taaataaaat catggctggc gtacgtaaag aagaaattca acttgaaaca acccatttag
      901 tagaatatat ggatgataga tatccatttt acttagatcc aatgcccaac ctttatttta
      961 caagagatcc ccaagcttca attggtagag gaatgacaat taacagaatg tattggagag
     1021 cacgacgtag agaatctatt tttatgacat atatactgaa acatcatcca agatttaaag
     1081 ataaagatgt accagtatgg ttagatcgta actcaccatt taatattgaa ggtggagatg
     1141 aattagtatt atcgaaagat gttttagcta ttggtatatc agaacgtaca tcagctcaag
     1201 caatagaaaa gttagcacgt aatattttca aagatgcaaa cacaagtttt aaaaaaatcg
     1261 tagctattga aatacctaat acacgtacat ttatgcacct agatacagta ctaactatga
     1321 ttgactacga taagtttaca gtacatgcag caatatttaa agaagaaaat aatatgaata
     1381 tatttaccat agaacaaaat gatggtaagg acgatataaa aattactcgt tctagcaagt
     1441 tacgtgaaac acttgctgaa gttttagaag tagaaaaagt ggactttatt ccaacaggta
     1501 atggcgacgt tattgatggt gcacgtgaac aatggaatga tggctcaaac acattatgta
     1561 ttcgaccagg ggttgtggtg acatacgatc gcaactatgt atcaaaccaa cttttacgcg
     1621 acaaaggaat taaagtgatt gaaattactg gtagtgaact tgtacgtgga cgcggaggcc
     1681 caagatgtat gagtcagccg ttatttagag aagatattta aatttgaaag gtggtaaaag
     1741 tcacaagtaa ttcattcatt aaatcaaata acaagatttt taccaccttt attaggggag
     1801 attaatatgg atgaaaataa attaggtaaa acttccttaa ttggtttagt cataggctct
     1861 atgataggcg gtggtgcatt caatatcatc tcagatatgg gtggccaagc tggtggactt
     1921 gcaataatta tcggttggat aataactgct attggtatga tttctcttgc tttcgtattt
     1981 caaaatttaa caaatgagcg accagatctt gatggaggaa tttatagtta tgctcaaaca
     2041 gggtttggag attttattgg tttttcaagt gcttggggat attggtttgc agcatttcta
     2101 ggtaatgtgg cttatgcaac cctattaatg tcagctgtgg gtaacttttt ccctatattt
     2161 aaaggaggta acacacttcc aagtattatc atagcatcaa ttttattatg gggtgtacat
     2221 tttttaatac ttagaggtgt agaaactgca gcgtttataa atagtattgt tacagtagct
     2281 aaattaatac ctatatttct agttattata tgcatgatag ttgtattcaa cttcagtact
     2341 tttaaatccg gtttttatgg tatgactagt ggaagtgttg gcgtttttag ttggggagat
     2401 acaatggcac aagtaaaaag tactatgtta gtaactgtat gggtattcac agggattgaa
     2461 ggagccgttg tcttttctgg acgtgcaaag tctaaaaagg atgtaggaac tgctaccgtt
     2521 attggtttga tttctgtgct agtcatttat ttcttaatga ctgtactagc ccaaggtgtc
     2581 attcagcaga accaaatttc aaaacttgct aatccatcaa tggcacaagt attagaacat
     2641 attgtaggtc attggggttc agtgttagtt aatataggct taattatctc tgttttagga
     2701 gcttggttag gatggacatt actagctggt gaattaccat tcattgtagc taaagatgga
     2761 cttttcccga aatggtttgc taaagaaaat aagaataaag ctccggtcaa cgctttaatt
     2821 attactaata tattagttca gttattttta attagtatgt tgtttacaga tagtgcctat
     2881 cagtttgcgt tttcacttgc atcaagtgca atcttaattc catatacact cagtgctttt
     2941 taccaggtta aatatactat tcaaaataaa tctaaagcta atttaaaaca atggataata
     3001 ggaattattg catctattta cacaatttgg ttggtttatg cagctggatt agattattta
     3061 ctattaacga tgttgttata tatacctgga ttactcgtat acagctacgt acaaagggat
     3121 aataacaaac atttgacaaa attggattat acgttattca tattcatcat tgtacttgca
     3181 ataataggaa tagttcgttt gattacaggt aatatttctg tattttaagt tatgatttaa
     3241 actaagtaat ttttaagggg tttatagtta tgtatgaaga aaatatttat attaaaaatt
     3301 cagaatatga atttgataat aatcttaaac aattagcatc atacttaaat attcctgtta
     3361 gtattgttag accttataaa gaggatttaa cactttatca atataaaaaa ggacaagtca
     3421 tatatcattc aactgatcaa ataaaatttg tatacttttt agtaaatggt tgtattttac
     3481 atgaatcttc taatattact ggtgacaatt atttaagatt aagtaaagac gaaaatatat
     3541 ttccaatgaa cttcatattt aatgaaaccc ctgcaccata tgaaatatgt acagctttga
     3601 cagattgtaa aatattaact ttaccgaaag atttacttga gtatttatgt agaaagcata
     3661 atgaaatatt tgaaagtctc ttcaagaaac ttaatgagac tattcaattt caagtagaat
     3721 atattatggc gttaagagct aattcagcta aagaaagaat tgaaagaata ctacaaattt
     3781 tatgcctttc aattggggat gataatggag aattctatga attaaaacaa attatgactg
     3841 ttcaattaat aagtaattta tctggactta acagaaaaac tactggtgaa ataatcagag
     3901 aattaaaaat agaaaatatt atatatcaag ataaaagaaa ttggattata aaaaaataac
     3961 atgaataaaa tattaattga aaaggagata ttcaaaatga aaaatttaag aaatagaaac
     4021 tttttaactt tgttagactt cacacaaaaa gaaatggaat ttttacttaa tttatctgaa
     4081 gatcttaaac gcgcaaaata tgcaggaata gaacaacaaa aaatgaaagg taaaaatatc
     4141 gctctacttt ttgaaaaaga ttcaacacgc actcgatgtg catttgaaac agcggcttat
     4201 gatcaaggtg cacatgtaac ataccttggg ccaacaggtt ctcaaatggg taaaaaagag
     4261 tctaccaaag atactgctcg tgttttaggt ggaatgtatg atggcataga atatcgtgga
     4321 ttctcacaaa gagtagttga ggatttggca aaatattctg gcgttcccgt atggaatggt
     4381 ttaacagatg aagatcatcc tacacaagta cttgctgatt ttttaacagc taaagaagta
     4441 ttgaaaaaac catataatga aattaacttc acatatgttg gagatggacg taataacgtt
     4501 gcaaatgcct taatgcaagg agcagcaatc atgggcatga ctttccatct tgtatgtcct
     4561 aaagaactaa atccaactga tgaattatta aatcggtgta atgatatagc tgacaaaaac
     4621 ggtggtgaaa tccttataac tgatgatatt gatgaaggtg tcaaaggatc tgatgtaatt
     4681 tacacagatg tttgggtatc tatgggtgaa cctgatgaag tttgggaaaa acgtatcaaa
     4741 ttattagaac cataccgtgt aactaaagaa ttaatgaaaa aaacaggtaa tccacataca
     4801 atttttgaac attgcttacc atcattccat gatactgaaa caataatcgg taaacaaatt
     4861 caagaaaaat atggtctaac tgaaatggaa gtaacaaatg aagtatttga aagtgaacaa
     4921 tcagttgttt tccaagaagc agaaaataga gcacatacaa taaaagcagt catggtagca
     4981 acattaggag agtaaatgaa aggaggatat aaatatgtct aaaatcgtcg tagctttggg
     5041 tggtaacgct ttaggacaat cgcctaaaga acaattagat ttattaaaaa gtacatctaa
     5101 atctctagta agtttaatcg ataaaggata cgaaattgta attagtcatg gtaatggtcc
     5161 acaagttgga agtattaact taggtttaaa ttatgcggcg gaacacaaac aaggtcctcc
     5221 ttttcctttc cctgaatgtg gtgctatgag tcaagcttat attggctatc agatgcaaga
     5281 aagtttacaa aacgaacttc attcaatggg catagataaa caagttgtta cgctagtaac
     5341 acaagttcaa gttgcaagcg atgattctgc tttcaataac cctactaaac caattggatt
     5401 attttataca aaagagcagg ctgataaatt tacgaaagaa aaaggttata cttttgtaga
     5461 agattctgga cgcggctatc gtcgcgttgt cccttcccca caacctataa gtatagttga
     5521 gttagatagt atagaaacat taatcactca tggaacacta gttatcgctg ccggcggtgg
     5581 tggaattcca gtaattaagg aaaatgaagt ttatacaggt gttgatgcag ttattgataa
     5641 agataaaacg agtgctttat tagcagcaca tttacaatct gatcaattaa tcatattaac
     5701 tgctgtggac catgtttaca ttaactatgg aaaagaaaac caaagaggtc tcgatgaagt
     5761 gtctgtggat gaaatgaaaa aacatatctc tgatggtcaa tttgctaaag gaagtatgct
     5821 tccaaaagtt gaagctgcac ttcaatttct tgaaaaaaat actaaaggca gtgttttgat
     5881 tacatctcta gcaggattag gggacgcttt agacggtaaa ataggaacat taattaagaa
     5941 ttgaaattat aatactttag ttcaattacc tttataaaca aaagagccta taatcctagg
     6001 gcactgaacc cttaaaacgg gaacttaata aaaacaccat gttctaggct attaattctc
     6061 tgtattttac aggggataag tagcctagtt tttgttgaat tcgattatta ttattattat
     6121 tattgttttt aatgtacttt tcgacaatat ctattacaat gtgattagag tgattaagct
     6181 cattattgat gtaaaaagtt tcagacttta gcgaggaatg gaaactttct atcggggcgt
     6241 tatcggcagg tgttcccttt cgggacatac ttctgataat gcctttttct tcgcacaatt
     6301 gataataagc ataagatgta taaacgctgc cttgatcact atgtaatata caaccctcag
     6361 gtatatcgat ttgatttaat gtatcattaa ctaaactttg gtcttgttta tcatctattt
     6421 tatacgccac aatttctccg ttataaatat ccattatcga agataaatac aacatagaat
     6481 gaccaaatgg taaataagta atatcggttg ttaatacttc cattggacaa ctcgctttaa
     6541 attgtctttg taataaattg tccgttttat aatacggttt acctattttt gtcatctttt
     6601 taggtctaac tcggcagttc aaatgatgct tctgcatcat tctctgtact ctcttatgat
     6661 taattggtga tgtataacat tgattaatca atgctgtaat cttacgataa ccgtaggtat
     6721 agtggttagc tttacataat tcaataactt tttgtgttac ggtatcattt ttatgggttt
     6781 tgtttttcca tcggtaatat gttgatttag gtatgtttaa tacttttagt agcaatttga
     6841 ttgaatattt tacttttaat tgatccacta aatctatgac tactgttggt accacttcct
     6901 ttccaatacc ttgtactttt ttaaaatatc caattctata tcttttctct tattttctaa
     6961 ttttaattgt tctacttctg acagctcttc taatccttta ccgtaggtat attgtttacc
     7021 aacttgttgt gaaaatctat atgctagttt tggtaaaact gaggaaggac gctggatagc
     7081 taagaatgcc cataattatg gatttattat aagatatcct aaaaacaaag aaagcatcac
     7141 aggatatcaa tatgaacctt ggcatttacg ttatttagga aaaagaaggc gacaaaagtg
     7201 tacaaatctg gtgaaacatt agagggattt gtaggcttaa aatgatcaat ttaaaataat
     7261 cgaaacttca actaccttat tatcgacgaa gtgtattcca gcacttcgtc tttattttta
     7321 ccgcaaacat tataccttac tatttaataa atctatttta tatctacata cttcctcttt
     7381 ctttttctaa aaatttttga gaattctctt tatcaatttc aataacactt atctaatgaa
     7441 acagtcattg tcgtaatttt ttcataacta cacgaggctt atattgaaat tgctaaagaa
     7501 catcccacga tgatttttta tcgggaaagg aatgggattt gcagattaga agaagcagaa
     7561 ggattgtgca agcacaatcc caaaatcccg ttattcttta taaataaata atatatataa
     7621 ggaagcagag accccaccct atcatcaata cttataaata atgagttgat tgttcgtcaa
     7681 aataggcgga atttcgggat ttccttgtca taccaaggtt tgtagcggtt ttttatatgg
     7741 aattttctca tattgtcaac gggatcagta ataaatgaca aatcaaatat atattatctc
     7801 aatgatgtat atgaagtaca tcactatcac actatctagg gaggcaatga tatgaaaaca
     7861 actactcaag aactcaaaca atatatgact cgcttattcc aattatctaa caatgaaaca
     7921 tgggaatgtg aaacactgga ggaagcggca gaaaatattc tacccaaacg ttttattaat
     7981 gattccccac ttgcacatct tatacttgaa acttatacct actataataa tgagctacat
     8041 gaactaagta tctatccatt tctaatgtat tctaataacc aactcatcag tattggttat
     8101 ctggatcatt ttgatatgga ctttctatac ctcacagata ctaaaaatac gattatcgat
     8161 gaacgtcatt tactaaaaga aggagggaat aaccatgaat aattggatca aagtggcaca
     8221 aatatctgtt acagtcatta acgaagtgat tgacatcatg aaagaaaagc aaaatggagg
     8281 aaaatagtat gaaaatcaat cgatacatca caagaggcat tagtgaacaa ctatctctag
     8341 accttcaaat cttactttgg aacatggtaa aagatcgaga caatcaactt aatacagatt
     8401 acctacacat tttcaaacta caagaagatg ataatatgtt gtcaattaca catgaacaag
     8461 aacaacccgc atacaaattg gaatatcact atacaaacta tataaaaaat caaaatgcat
     8521 tacctaagaa agtctacgtc atccgagaag atgatgtaga cgctttttat tatgtgatgc
     8581 ttttgccaga agaatactaa aggaagtgac gatatatgaa tacaatcaaa agtacgatac
     8641 acacagaagc gatatttagc gatgatgaac aacatcgcta cttactcaaa aagatatggg
     8701 atgacaagaa accggcttgt actgtgataa cgatgtatct tcatttagat ggtgtattat
     8761 cactcgatct tactacggtt ctcatcctca atcaattagc taattctgag caatatggcg
     8821 ctgtttatct cgttaatctt ttctctaata ttaaaacacc agagaacctt aaacatatca
     8881 aagagcctta tgatgagcac acagatatac acttaatgaa agcaattagt gaaagtgaca
     8941 cagtcattct tgcttatggt gcctatgcga agcgaccagt tgttatcgac cgtgtcgaac
     9001 aagtgatgga aatgttaaaa cctcataaaa agaaagtaaa aaagctcata aatccagtaa
     9061 cgaacgaagt tatgcatcca ctcaacccta aagcacgtca aaaatggaca ctaaagtaaa
     9121 ggagtattat ctatgaacca tgacaacact atacaatcca attggcgaac ggttgctagt
     9181 tgtttagcat cacaaaatta catttcaata gtaaaaggtc tggtgcatca tttcacagcc
     9241 atcgatgacg aggaaatact tgataaaatc tatgaagagt ttataaatga tgactctatt
     9301 acaacggtac ttaacaatga tttacaaaca attattaatc attatctatc aaaatgaaaa
     9361 ctatattcta ataaaactag agcatccttc acttttgcgg tgaaggatgc tcctatatct
     9421 ttttttatct aaaaaaacta ttagtaatta gttttaaaac atttatcttc taatatatag
     9481 atatgaaatg taaaaataca attagattgg cagtcacaat gcaaatgtaa tatttgtcag
     9541 taatcttcct actcaaattg ttattctttt catagaaatc aagaggagga acgacttgaa
     9601 ttctattata ttcaatgcat cttcattata attctaccta cttcaccatt agacaaacga
     9661 tcaaatccct cattaatttc atctaaagtg ataacctcac tgatgagtga atcaatattt
     9721 aaacgtcctt ggttgtatag attaacgaac cttggtatat ctctgtcagg tacacaactt
     9781 cctacatatg atcctttaat ggtacgttct tcagccgcta aagtaacttg agggaaagaa
     9841 aaattatctt taggattagg cagtcctgtt gttacagttg tcccccctcg tttagtaatc
     9901 tgataagcaa ctttcatagc tggcactaca cccgctgttt caaaagcata ttctactccg
     9961 ccaggaatat attctttaat ttgctcatcg atatctttat cgcttgaatt aaatgttgct
    10021 gtagccccaa attgctttgc taattcaaat ttatcttcgt taatatctaa agcaataatt
    10081 tcattggctc ccgctagttt agctccaata atagcattca atccaatacc tcctaaacca
    10141 acaaccgcta cattactacc agaacgaatt tgggctgtat tcaccacagc accgatacct
    10201 gtgataatag cacaaccaaa tgcagctgca cgttcaaaag gtattttttt atcaatttta
    10261 actatagagt tttcagagac aacagaatat tcagcaaaac ctgatatccc caagtgatga
    10321 tatacttttc cttcatcatt agataaacgc atccctccct ctaacatttc gcctttttca
    10381 ttagatattg ctccgttttc acataatgca ggacgtcctt ctttacatgg gatacatttt
    10441 ccacaactgg gaataaatgt gcataccaca tgatctcctt cgctaactct tgtaacagct
    10501 tttccaactt taattacttc accggaagct tcatgaccaa gtgccatagg taaaggtcta
    10561 ggtctgttac cattaattac tgacaaatca gagtgacata atccagctgc atgaatttta
    10621 attaatactt cgtgttcgct aggattatca agttctaagt attcaatttt taaaggttta
    10681 gattctttat agggcttttt agcccccatt tcatgtaaaa ctgctgctct agttttcact
    10741 tccaacactc ctttaattta tatacattaa gtctattctt atctgttatt ttacacttta
    10801 atattattat ttacaaaaaa ttctgattat tgagattatt ttatataata ccaattttta
    10861 aaaatttatt atgtaaaatc ataatttaga agtgatttgg ttttcaagtg aatttaaggt
    10921 gtgaagatta aaaaatatat taaagaaata cttgataaaa tttatgaaga ttttatgaat
//
LOCUS       DAKTGS010000096        10972 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.096, whole genome shotgun sequence.
ACCESSION   DAKTGS010000096 DAKTGS010000000
VERSION     DAKTGS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.096"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(183..638)
                     /locus_tag="O0286_001983"
     CDS             complement(183..638)
                     /locus_tag="O0286_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCY0170351.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            complement(631..2781)
                     /locus_tag="O0286_001984"
     CDS             complement(631..2781)
                     /locus_tag="O0286_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCY0170352.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            complement(3215..3985)
                     /gene="sigB"
                     /locus_tag="O0286_001985"
     CDS             complement(3215..3985)
                     /gene="sigB"
                     /locus_tag="O0286_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCY0170353.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            complement(3960..4439)
                     /gene="rsbW"
                     /locus_tag="O0286_001986"
     CDS             complement(3960..4439)
                     /gene="rsbW"
                     /locus_tag="O0286_001986"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCY0170354.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(4441..4767)
                     /locus_tag="O0286_001987"
     CDS             complement(4441..4767)
                     /locus_tag="O0286_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCY0170355.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(4886..5887)
                     /locus_tag="O0286_001988"
     CDS             complement(4886..5887)
                     /locus_tag="O0286_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCY0170356.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            complement(6237..6599)
                     /locus_tag="O0286_001989"
     CDS             complement(6237..6599)
                     /locus_tag="O0286_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0170357.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(6596..6766)
                     /gene="mazE"
                     /locus_tag="O0286_001990"
     CDS             complement(6596..6766)
                     /gene="mazE"
                     /locus_tag="O0286_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCY0170358.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(6851..7999)
                     /gene="alr"
                     /locus_tag="O0286_001991"
     CDS             complement(6851..7999)
                     /gene="alr"
                     /locus_tag="O0286_001991"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0170359.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(8065..8424)
                     /locus_tag="O0286_001992"
     CDS             complement(8065..8424)
                     /locus_tag="O0286_001992"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCY0170360.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(8428..8919)
                     /locus_tag="O0286_001993"
     CDS             complement(8428..8919)
                     /locus_tag="O0286_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0170361.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(8906..10489)
                     /locus_tag="O0286_001994"
     CDS             complement(8906..10489)
                     /locus_tag="O0286_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0170362.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(10482..10961)
                     /locus_tag="O0286_001995"
     CDS             complement(10482..10961)
                     /locus_tag="O0286_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170363.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
BASE COUNT         3389 a         2114 c         1427 g         4042 t
ORIGIN      
        1 gccgagattg cgaagtatta aattacgaca atctcggctt taaacatttt ataaacacaa
       61 tttcttctta ttcaattact atgtttacca atcaaaataa ctcactgata aattctatat
      121 aaattccata caactcataa ataatcttac atatacacga attagctcta tttgtattgt
      181 acttatttca attgtctttt cattcttaat tttccattgc aatgtccgca acgcatacga
      241 ttcgtatcaa ctttacggat acgtatatat tttgcatgac atttagtgca ataatattca
      301 taatttgccc gttgttgata gctttcaatg ctgttacaaa atctaggcgc tccaacctgt
      361 tggctcaatc gtttaaaatc ttgatcttta tgttgataac ctttaccagc aatatgcaag
      421 tgataatgac acaattcgtg cagtataatt tttacaacag catcttctcc ataatgctca
      481 tattgttttg gattaatttc aatatcatgg gactttaaaa gataacgtcc gcctgttgta
      541 cgtaaccttt tattaaaata tgcacaatgt cgaaacgtac gtccaaattt ttcttccgaa
      601 agattctcaa ccattcgctg aagtttgtca ttattcatgt ggatcaatca tcgttaatga
      661 tactttgtct ttatttttgt caatactgta aatccaaacg tcaacgatat caccaacact
      721 gacaatatcc attggatttt ttacgaactt cttagaaagt ttcgaaacat ggacaagtcc
      781 atcttgttta acacctatat ctacaaatgc tccaaaatct actacatttc taactgtacc
      841 actcaatttc attccttctt gtaaatcttc aattgatagc acatcggatt taaggattgg
      901 tgtttcaaac tcgtcccttg gatctcgatt aggtgctttc aaggatttaa taatatcctc
      961 taatgtaggt acaccgactt gtaattcaat cgccagttca tccatatcca atgaatttaa
     1021 tttagctttt aatgcatcac tacctaaatc attgccacca aatccgagtt tatctaataa
     1081 ctgatacgtt actttatagc tttcgggatg aatcgatgta ttatccaatg gctctgaacc
     1141 gtctactatt cttaaaaatc ctatactctg ctcaaaagtt ttagcaccca aacgcttaat
     1201 cttgcttaac tctttattat gctttattgc gccattttct tctctatatg caattatatt
     1261 tttagcaatt tgcgaactta gaccagacac atattgtaat aatgatgatg atgctgtatt
     1321 gacatcaaca cctacttggt taactgctgt ttcaacaacg aaagttaatg cattttctag
     1381 tgctttttga ttaacatcat gctggtattg tccgacacca atagatttag gatcgatttt
     1441 aactaattca cttaatggat cttgaacgcg tcgtccaatt gatacggcac ttctttcttc
     1501 cacttgaaaa tcaggaaatt catctctagc aatttctgat gctgagtata ctgaagcgcc
     1561 cgcttcattg acaatgatga attgtactgg caactgatgc tttttaatta aatctgcaac
     1621 aaattgttct gtttcacgac ttgcagtacc attgccaatt gcaattaatt gcacatcata
     1681 cgctttaacc atttgtacaa aatccttctc tgctgcctct tttttagaaa ctggtggatg
     1741 cggataaatc acaccttttg ctataaaagt accgaatggg ttaatgactg ctaatttaca
     1801 acctgttcta aatgctggat ctacgcctaa tatttgttta cctttcattg gaggttgcag
     1861 taatagattt cttaagtttt cactaaaaac atctattgca tgattttcag ctttttcagt
     1921 taaatcagca tggatttcac gctctatcga agggacaatt aagcgtttca agctatcttt
     1981 aatcgcctct aaaatataac tgcgatttac attattatga ttgatttctt gacgtgcaat
     2041 gaaatcctct actgatgtcg tatcgaattc aaactttaca gataatactt tctctttttc
     2101 accacgatta acagctaaaa ctctatgatt agcaatgcgt ttaattggct cactatatgc
     2161 atagtacatt tcaaaaatac ctttttcatc ttcagcattt ttctttttag atgtagttaa
     2221 cacaccttga tgatacatat cttttaaaat ttttgttcta tatttaggat tatctgaaat
     2281 ttgttccgca ataatatctt gtgcaccttt gatagcatct tcaaccgatt gcacttcttc
     2341 atttataaat tgttgtgctt tttcttcaat tgagacttca tgtttacgtg ccttcatcca
     2401 tatcgctaat ggctctaacc ctttacgttt cgcctcagtt gcccttgtct ttttcttttg
     2461 tttaaaaggc ctatataggt cttcaacacg ttgtaatttg ttctgtttta aaatatcctt
     2521 ctttaattcc tcagtaagta atccttgctg ttctatattt ttgataactt cttctttacg
     2581 tttttgtaaa ttgaccatat attggtattc gtcatcaatt tgctttattt gaacttcatc
     2641 tagtccacca gtttgctctt ttcgatacct cgcaataaat ggtactgtat ttttttcttc
     2701 tagcagtgtt aatactgctt caatttgttt tttactaaat tgatatttct ctatgattga
     2761 attaatcaat tgattgtcca tttacttaac ccacactttt tcttaaattt actcatttat
     2821 tttaacatat tctaaaatac ttctattaag gtatgatact taatgtaatt ttcacttcca
     2881 aaacatttaa aacgaatgat taggtcatac tatattttaa aacgcactaa gattattgaa
     2941 aatgacacac catcatttct aaatttcata atctaagact acttttttta ttctaatagc
     3001 aacccaccta tttaaaacga caattagata tcttcttcac ttaaattatt aatactatat
     3061 cgcgacattt atgtggatac acattaaact acactaataa tgtattaatt cgaaaatctt
     3121 aattaaatta aaaatacccc tcgatttcaa tttaataatc aagggataat tatatggatt
     3181 tttcattata aaacgtatca ttaataaaca aattctattg atgtgctgct tcttgtaatt
     3241 tcttaattgc cgttctctga agtcgtgata catgcatttg acttaaaccg atacgctcac
     3301 ctgtctcttt ttgactcaat ccttcaataa acgtacattg tatgatttct cgttcgcgat
     3361 cagataatat aggtaatatt ttttctaaaa tcatacgttt ttcagttaag tcataatggt
     3421 catcttgttg ccccataata tctaatagcg taacagttga accatcttta tcagcttcaa
     3481 tggaatgatc aacacttaac gcattataac tttgtcccat ttccattgct tctaacactt
     3541 cttcttctga gacttctaat cgatcagcta tttcactgat agaaggtgaa cgctctaatt
     3601 cagcggttag ttcatcgctc acttttttga ttcttggccc aatttcttta atacgtctcg
     3661 gaacatgtac actccaagtt ttatctcgta gatatctttt gatttcaccg attacagtag
     3721 gtactaaaaa ggcttcaaac ttccgttcaa aggacatatc gaatctattt atggcaccta
     3781 ttaaaccaac cataccaact tgaactaaat cttcgtgatg tgattgtcct ttagaatatt
     3841 tatatgccaa tgactcaatt agtttttggt aatgtttaac taacttatcc tgtgcatctg
     3901 tattcttatt ttcttggtgt tctttaatcc attggttaat ttgctcaggt gaaatttcat
     3961 tagctgattt cgactctttc gccattattt cgcacctgct ctttttttat atacttagtc
     4021 atactgattg tcacaccaga ttctttatat actgtgactt catccattaa agattcgatt
     4081 aaaaataaac ctaggccacc ttcgcgtaaa aagtctatat tttcgtcttt atcgtaagga
     4141 cctatttttg atttagttgt ttcataatca aaactgtcac ctttatcaga aataacaatt
     4201 ttaattttat cttctaaaat ttcaaaatat atgttaataa tgcccacatt gttattttct
     4261 ttgtatgcat gtttaactgc atttgtcaca gcttcactaa ctgcaatctt ggcatcttca
     4321 atatcatcat atgtagcacc agctctcgaa aaaacgccag aaagtgttaa acgaattaaa
     4381 cttacatact ctgccgatgc tggcacgcgc atttcgataa aatcttcttt agattgcatg
     4441 ttattcgacc tccgttcctt cattaacatg cattaaatcc ttaagaccag taatttcaaa
     4501 tagtctaccg atacgatctg acacacctaa aatgtatagt tctttatcat tttggtttaa
     4561 tgcttttaat gtacctacga ataaacctaa acctgtcgaa tccatataac tcacattttc
     4621 taaattaaca taaatatcac gagttccatc ttgtctcata ggtgttaaaa cctcttctaa
     4681 ttcaggcaca gtataaacat ctaattctcc accgacttta acttcgtaaa atttatcttg
     4741 agtggttgtt tctatattaa gattcatttc attacactcc tacttattaa aaatatttat
     4801 cttcatttta agcgctgtat ccaccatatt tatatttcaa attattatat acccatcgaa
     4861 ataatttcta atcttctttt ttaaattaat ttactctttt tataatcaaa atagtcatat
     4921 catctttttt atttgggttt tgaagcttta aaattgcttc atagataatt tgaacaatat
     4981 cttgtgggtg catatgttta tgttttttaa tatattctaa aagtttttgt ttatctataa
     5041 aggtaccttc actatttcta gcttcagtca caccatccgt taaaatgata attaaatcat
     5101 caaggtatat tggaatttct tgttgttgat atcgtgtttg tgaactgatt cctaacactc
     5161 tacctctaac tgaaatttct tcaaattctt ctttttcagc gcgataaata tatccaggct
     5221 catgaccagc tgaactacaa tacaataaat ggttcatttc ttcatataaa ccataaaaca
     5281 ttgtgacgaa catattttga ttaatattct tttcaacaac acgatttaaa cgttttaaac
     5341 catcactcgg taattgtgag tgtccataag aatccatgcc aaactttatc atactcattg
     5401 ctaaagcagc tggtatacct tttccaatga catctgcaac agcaaagctc attgtgccat
     5461 cgttatggtc aattaaatta aaataatctc cacttacttt ttgtgccgcc actgaaataa
     5521 cgccaatttg aatactatca aattgtggaa tatctgtttt aagcattgtt tgttgtaagc
     5581 tagaagctaa gtctatctct ttatcgtgaa cttgaagttt atctaccaat ctttgataat
     5641 ctcgataact ataaccaaag cctttaacga tttcttgtaa gacatctaat gtcttgaaca
     5701 catcttcacg cgttaagttt aacgtcaata tatagttttt gtgaatatcg acaatgtctt
     5761 caggcaagac gtccttacga atcacttcgt cagtgtattt ctcacacttt tttatcaatt
     5821 ctactttatc ttggcacgtt aaactttcat caattaaacc cttataatgt tgcttaaatt
     5881 cttccacgat tagttgcctc ctcacttccc ttacaaagca aatgcttaag caaggataat
     5941 tatgattcac ttactaacat ttatttaatt gcacttatca attttgtcgt ttgcaatttt
     6001 gctgtagatg atacttttaa gctaaaaaaa actttaggat ggaataccta aagcctaaaa
     6061 aacttaagac atccttttat aataaaaaat atgaatatca taactggtat ttgctaaaat
     6121 ctttgtttat aagaaaattc ttcgtacaga atttagaaat agttataatt attataaagt
     6181 tattgtcttt tatatcaata ttttatttat ctctgaaaaa tacatataat agacgcctaa
     6241 tttttctggt gagctactgc attcagccct aaactaatca ttagtgcatt atctacttct
     6301 ttcattttat catcggataa gtacgtcagt ttttctttca atcgtttttt atcaagtgta
     6361 cgaatttgtt ctaataatat aactgagtct ttatccaact tatacttttt cttttcaatc
     6421 tctacatgtg tcggtatttt cgctttatta atcctaccag ttattgccgc aacaataact
     6481 gtaggactat atttattacc agtatcattt tgaattatga ctacaggtct gactccccct
     6541 tgttcagatc cctgtactgg tgataaatct gctaaataaa catctcctcg tctaatcatt
     6601 cattcgttga attagaagat aaatatgttt cgttgcaatc gcatgcttca cactctatcg
     6661 gaaaagcttc gttcgctagg gagagattta aatcagccat ttgtgaatat ccttctttta
     6721 aagattgttc taagctatga cttctatttt gactaaaaga taacatatga gaacctccaa
     6781 ttgattccaa aagttattaa atgaccttta attactctaa tgataacaaa ttttatatgc
     6841 actgaccata ttattttaac aattcatttg ttacaaaacg ttgatcacca tcatgataga
     6901 ttcgcggcaa acgtctcgac aagttacaaa gcacttcata attaatagta tgttgctttt
     6961 cagctaccac ctctaccgac tgtggacttt ctctatgatt atctattaaa atcaccgaat
     7021 ctccagcttt aacttgatct ggcactttaa caattgtctg atccatacat acgcgaccaa
     7081 taacttcgca ttgatgacca tttacattta caaagctacc ttgcattatg cgtaaatagc
     7141 catctgcata tccaataggt aacaatgcta ttgtagttgg gtcagtagct gtataagttg
     7201 caccataact tacagactca cccgcttgta gcgtctttgt ttgaactaca ttagcaatta
     7261 attgcacact tggtttaagg tgtactttaa ctttttgctg tacatactct gatggataat
     7321 atccataaag ggaaattcct ggtcttattg cattacagaa ttggcaatcc attaatagag
     7381 agcctgctga gttctgacaa tgtatatatt caggtttaat tgcttcattg accatatctt
     7441 taaaacgttg atattgttca gttgtcatat ctcctggttc gtcagcacag gcaaagtgtg
     7501 taaacacgcc ttcaaataca agttgctcat attgttgaat gatttcaatc acttcttgat
     7561 acgttttagt atctttaata cctaaacgtc ccattcctgt atctaattta atgtgcaacc
     7621 ataacttttt ctcttgctca ccagaaatgt ttttaattgc ttctttcaac cactgtttag
     7681 acggaaccgt taaggcaact cggtgttgta tcgctttatc aatatcttta gctggtaaca
     7741 cacctaagac taaaatttta gcagtaatcc catgcattct aagttctatc gcttcatcta
     7801 acgttgctac agcaaaaaat gtggcgccat tttccattaa atgacgtgct actttaacac
     7861 tacctagtcc ataggcattg gctttaacga cagccatcac tgttttattt ggatgcaatg
     7921 tactgaatac tttgaaattt gatgcaacag cgtttaaatc tacattcata tacgcagatc
     7981 tataatattt atccgacata ttacttcctc ctgtaattcc cacacgtttt aaaactagat
     8041 cttaattatc attgtataac aaatttaaaa tgctgacttt tctaaaacaa cttggctcat
     8101 cgcataatgc tcagtgtgtg agatactaac atgtacgata aacccttcgt aatcaatctt
     8161 tggtttgcca agttcgtcgt tgtaacagtc tatatcgtta aaagctacat gttttcctaa
     8221 gcctgtgcct aatgctttac tgaacgcttc ttttgtagca aacctgccag ctaaaaattc
     8281 aattttacgt tgctcatgtg tgaaattgtt gaatttgtgc tgttcatttt tagttaaaat
     8341 ccgctcaacc aattttggtt gcttactata taacgcttgt attcgatcga tttcgattaa
     8401 atctacacca attccatgta tcattgatta ttcctcattt ttattgtttt ctaaatgtct
     8461 ctcgttattt aaccatcgtt tcaatctacg tgcttcactt ctatctatta attcaatttc
     8521 agcttgacca cccgctgtat aaagggtcaa agttgctaat cgatagcgtc tcattatagg
     8581 accaacatcg atatcaatat tttgaatacg aaaatatggt attacctttt catccaaaaa
     8641 taaaatgccg tttcgtacac gcaaatggtg tttttcaaat gcatatctgc agtgcttata
     8701 tcgatagact ggcgctataa caagcgtgaa aacagcaaca agtaatatta taatcacact
     8761 actaacaatg gataaatggt tatctaaaat ctcccaaaat agccagttca aaatattaaa
     8821 tgcgattaaa agtacaagcg ctatgggtat ccaaaacagc acagcactta acctcatcac
     8881 ttttttagcg tgtggtgaca taaaattata atccctcatc attttcacct cttaaatacc
     8941 atgatttcaa tttatttgca tcttcacttc ttgcgtattt taagttaatc gattgggctc
     9001 ccacaccttt agcaacaata aagctaaaat tatttaaatt gttgcgttta agtaatgtat
     9061 gttgccaagt gtcaaatcct ataatgtgat gcgctttaaa ataataaata tttcgtttca
     9121 atagctcgaa attctggata gtaatttgtt cttctgtcat tttaaaaccc gcatgtttga
     9181 cataaagata tcctttgatc acaaataaac caataatgac tattgttata atcgtaaata
     9241 acaataataa ttgattccaa aaataacagc ctatacctgc catagctatg acaataatac
     9301 taggtattaa aaagtgtctg tggaaacctg acaaaggcat accttcatta acttgttgat
     9361 aagataaatc tggtactaaa ttctggataa tttgatatgc tttgtctcgt ttaataaacg
     9421 gcaatatcgg cacactacct gaatcattgt catcatcttc gattttttta ttttcaaaat
     9481 cacttgtgat gataaaatgt attgctgtgt agccaaataa tcttcgtata tatgactgac
     9541 gttcaacaac agcttgcaat cgtttcgttg gtacagttac tttcttcttg ttaaagagac
     9601 catattcaat tttaagttgc tcattttcaa gcatcaccga aaagccataa aatcttatca
     9661 ttgttataat cgttccaata atatatgcca ctattaatac tagtaaaatg atgattaata
     9721 ctgaaatact tacaatttga acccattgac taatttcatg atttagcttc gaccatggga
     9781 tcaactctct tacagccccg taaatcggta ctaaagctgc taacgttaca ccaatggcgc
     9841 cactggtcat tgccataaat agtgattctt taaaattcat ctgatatata ggaatgcgtt
     9901 tatttttctg attaagcata ctatcagtgt tctgcacttc atctaagcga ccttctgcga
     9961 tgtcttccac attaccttca atgtcatgat tacagttgtc attcttctca gcactagact
    10021 tttgcgccac ttctgtcttc aactctgttt gcaattgatc aatatatcgt tcaagatatt
    10081 caccttgttt tttcgaaata acacttaaga caataccatc acttggtgtt ttgatttgta
    10141 aatccacacc accaataatt tgattgacga ccccttgtgt catatcaacg gactgaatac
    10201 gttttatatt taattcttta cgctttttat taaaaatacc tgttgttaaa atgaaataat
    10261 tatcctcaat ccaatatcgc gtgttcataa ttccgacaat ttgagaaatg tatgatatta
    10321 aaaagaatac aaatacaata cctatccata aatatgattc gggattcgta taatcaaaat
    10381 ctttcaattg aaagataatg aaaataaaaa agacgactat gttttgtttg atagcattga
    10441 ttatgccatt aaaatatgaa atcggatgta atttttgagg ttcagacatc actttcaacc
    10501 cctctcaaat tcgacatagt tctctcttcg attattttaa catcgtcatg agacatcatc
    10561 ggtaaataaa tagtatgacc tgcagtcata aatccaactt tatacaaatt aagcacttta
    10621 ctaattggat tagatttaat cgacaagtat tgtaaacgtt caattcgact cgtttcttct
    10681 ttatatataa aaaatgatgt acgatattgt acacttagtt gatcaacttt ataaaagcga
    10741 caatgatatt gccataaagg cttaataaat aattttaatg tactcagagc acctaaaacc
    10801 aacaaaatat aaagtaagta atgtggccat tcaaatctta accatataaa ataaaaaatg
    10861 acatacacag ctacactcaa tataaattct aagccattcg taatgtagta atacaacaat
    10921 gctgacttag gactcttagt caacttagta taatctgaca tatacccctc tc
//
LOCUS       DAKTGS010000097        10943 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.097, whole genome shotgun sequence.
ACCESSION   DAKTGS010000097 DAKTGS010000000
VERSION     DAKTGS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.097"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            103..762
                     /gene="arlR"
                     /locus_tag="O0286_001996"
     CDS             103..762
                     /gene="arlR"
                     /locus_tag="O0286_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCY0170364.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            759..2114
                     /gene="arlS"
                     /locus_tag="O0286_001997"
     CDS             759..2114
                     /gene="arlS"
                     /locus_tag="O0286_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCY0170365.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2398..5196
                     /locus_tag="O0286_001998"
     CDS             2398..5196
                     /locus_tag="O0286_001998"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCY0170366.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5210..6478
                     /gene="sucB"
                     /locus_tag="O0286_001999"
     CDS             5210..6478
                     /gene="sucB"
                     /locus_tag="O0286_001999"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCY0170367.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            7058..7867
                     /locus_tag="O0286_002000"
     CDS             7058..7867
                     /locus_tag="O0286_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0170368.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            7896..8099
                     /locus_tag="O0286_002001"
     CDS             7896..8099
                     /locus_tag="O0286_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170369.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            8280..9071
                     /locus_tag="O0286_002002"
     CDS             8280..9071
                     /locus_tag="O0286_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCY0170370.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            9085..>10943
                     /locus_tag="O0286_002003"
     CDS             9085..>10943
                     /locus_tag="O0286_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCY0170371.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPL"
BASE COUNT         4126 a         1569 c         1980 g         3268 t
ORIGIN      
        1 taaacgtaaa caaactaatt taaaaagcaa ctattgtata gaaaaataca aaatttaaaa
       61 tatattacct tattagaaaa agtcgtaata tgaggtgtac aaatgacgca aattttaata
      121 gtagaagatg aacaaaactt agcaagattt cttgaattgg aactcacaca tgaaaattac
      181 aatgtggaca cagagtatga tggacaagac ggtttagata aagcgcttag ccattactat
      241 gatttaatca tattagattt aatgttgccg tcaattaatg gcttagaaat ttgtcgcaaa
      301 attagacaac aacaatctac acctatcatt ataattacag cgaaaagtga tacgtatgac
      361 aaagttgctg ggcttgatta cggtgcagac gattatatag ttaagccgtt tgatattgaa
      421 gaacttttag caagaattcg tgcaatttta cgtcgtcagc cacaaaagga tattatcgat
      481 gtcaacggta ttacaattga taagaacgct tttaaagtga cggtaaatgg cgcagaaatt
      541 gaattaacaa aaacagagta tgatttacta tatcttctag ctgaaaataa aaaccatgtt
      601 atgcaacggg aacaaatttt aaatcatgta tggggttata atagtgaagt agaaacaaat
      661 gtcgtagatg tttatataag atatttacga aacaagttaa aaccatacga tcgtgacaaa
      721 atgattgaaa cagttcgtgg cgttgggtat gtgatacgat gacaaaacgt aaattgcgca
      781 ataactggat tattgttacc acgatgatta cgtttgtcac gatatttttg ttttgtttaa
      841 ttattatttt tttcttgaaa gatacactgc ataatagtga gcttgatgat gcagaacgaa
      901 gctcaagcga tattaataat ttatttcatt ctaagcctgt taaagatata tctgcattag
      961 acttgaatgc atctttaggt aattttcaag agataattat ttatgatgag cataataata
     1021 aattatttga gacatcgaat gataacacag tgagagttga accaggttat gaacaccgtt
     1081 attttgaccg cgtaataaaa aaacgctata aaggcattga atatttaatt attaaagaac
     1141 caattacaac gcaagatttc aaagggtata gcttgttaat tcattcacta gaaaattatg
     1201 ataacatcgt aaaatcattg tatatcattg cgctggcatt tggagtgatt gcaacaatta
     1261 taactgccac aatcagttat gtattttcaa cacaaattac taaaccgctt gtcagtttat
     1321 caaataaaat gattgagatt cgacgagatg gttttcaaaa taaattgcaa ttaaatacaa
     1381 attatgaaga aatagataat ttagcaaata cgtttaatga gatgatgagc caaattgaag
     1441 aatcatttaa tcaacaaaga caatttgttg aagatgcgtc acatgaatta cgaacaccat
     1501 tacaaattat tcaaggtcat ttaaatttga ttcagcgatg gggaaaaaaa gacccagcag
     1561 tattagaaga atcgttaaat atttctattg aagaaatgaa tcgtatcata aaattagtcg
     1621 aagaattact tgaattgact aaaggagatg taaatgacat ttcttctgaa gcacagaccg
     1681 tgcatattaa tgatgaaatt cgctcgcgaa tacactcatt aaaacaattg catcctgatt
     1741 atcaatttga tacggatctg acatctaaaa atctagaaat taaaatgaaa cctcatcaat
     1801 tcgaacaatt atttttaatc tttattgata atgcaatcaa atatgatgtg aagaataaga
     1861 aaattaaagt taagacaagg ttaaaaaata agcaaaaaat aattgaaatt acagatcatg
     1921 gaattggtat tccagaggaa gatcaagatt tcatttttga tcgcttttat cgagtggata
     1981 aatctcgttc aagaagtcaa ggcggtaatg gactcggatt atctattgct caaaaaatca
     2041 ttcaattaaa cggaggatcg attaaaatta aaagtgaaat taacaaagga acaacgttta
     2101 aaatcatatt ttaatcatga ctgagacgtc aatcaaagtc ataggatcaa ttttttaagt
     2161 acacattagc tgtgactaat gtataagaac aactataaaa caaataaaca gtggttcttt
     2221 atcatttctg ttgtactccc aaaatttaca ataaaataca tctataaacc tagaagaatc
     2281 aacgcttttg ttgattcttc tttttagcag ataaataggt aaatctactt taacaaataa
     2341 ctaaatagtg atattattac attgtaagcg tttcaacatt tttgtggagg gtgtaaaatg
     2401 actaacgaaa gaaaagaagt ttcagaggct cctgtaaact tcggtgcgaa tttaggtcta
     2461 atgttagatc tatatgatga ctttttacaa gatccatcat ctgtaccaga agatttacaa
     2521 gtcttattca gcacaattaa gaatgatgac tcaattgtac cagctttaaa aagtacaagt
     2581 agtcaaaata gcgacggcac aattaagcgt gtcatgcgtt taattgataa tattcgccaa
     2641 tacgggcatc ttaaagccga tatttatcct gtaaatcctc caaaaaggaa acatgtacct
     2701 aaattagaga ttgaagactt tgatttagat caacagactt tggaaggtat atcagcagga
     2761 attgtttcag atcactttgc cgacatttat gataatgctt atgaagcaat tttaagaatg
     2821 gaaaaacgtt acaaaggacc aattgcattt gagtatacac atattaataa caataccgaa
     2881 cgtggttggt taaaaagaag aattgaaacg ccatataaag taacgttaaa taataacgaa
     2941 aaaagggcac tattcaaaca attagcgtat gttgaagggt ttgaaaaata tcttcataaa
     3001 aacttcgttg gtgcaaagcg tttttcaatt gaaggggtag acgcacttgt accgatgtta
     3061 caacgtacta ttacgattgc tgcgaaagaa ggtattaaaa atatacaaat aggcatggct
     3121 caccgtggac gtttaaacgt tttaacgcat gtcttagaaa aaccgtacga aatgatgatt
     3181 tcagaattta tgcatacaga tccaatgaaa ttcttacctg aagatggtag cttgcagtta
     3241 actgctggat ggactggtga tgtgaaatat caccttggtg gcattaaaac tactgattca
     3301 tacggtacaa tgcagcgtat tgcactggct aacaatccaa gtcacttgga aattgttgca
     3361 cctgttgttg aggggcgtac gagagcagca caagatgata cacaacgagc tggggctccg
     3421 acgactgatc atcataaagc aatgccaatt attatacatg gcgatgctgc ttatcctggt
     3481 caaggaatta acttcgaaac aatgaactta ggaaacttga aaggctattc tacgggtggt
     3541 tcattgcata ttattactaa caatagaatt ggatttacta cagaaccaat tgatgcacgt
     3601 tcaacaactt attctacaga tgtggccaaa ggttatgatg tgccaatatt ccatgtcaat
     3661 gcagatgacg ttgaagctac tattgaagca attgatattg caatggaatt tagaaaagag
     3721 tttcataaag acgtcgttat tgatttagta ggttatcgtc gtttcggaca taacgaaatg
     3781 gatgaaccat caattactaa tccagttcct tatcagaata ttcgcaaaca tgactctgtt
     3841 gaatatgtgt ttggtaaaaa gcttgttaat gaaggtgtca tttcagaaga tgaaatgcat
     3901 tcatttatag aacaagtcca aaaggaacta agacaagctc atgataaaat taataaagct
     3961 gataaaatgg ataatccaga tatggaaaag cctgcagatc ttgcattacc gttacaagca
     4021 gacgaacaat catttacttt tgatcacttg aaagaaataa atgatgcatt gttaacatat
     4081 ccggatggct ttaacatttt gaaaaagtta aacaaagttc ttgagaagcg tcatgagccg
     4141 tttaataaag aagatggttt agttgattgg gcacaagcag aacaacttgc atttgcgaca
     4201 attttacaag atggtacacc gattcgctta actggtcaag atagtgaacg tggtacattc
     4261 agtcataggc atgccgtgtt acatgatgag caaacaggtg aaacatatac acctttacat
     4321 catgttcctg atcaaaaagc gacatttgat atacacaatt ctccgctttc agaagcagca
     4381 gtagttggtt ttgaatacgg ctataatgtg gaaaacaaaa aaagcttcaa tatttgggaa
     4441 gcacaatatg gtgattttgc aaatatgtca caaatgattt ttgacaactt cttattcagt
     4501 tctcgctcaa aatggggaga acgttcagga ttaacattat tcttacctca tgcatatgag
     4561 ggtcaagggc ctgaacattc atcagcaaga ttagagcgat ttttacaatt agctgctgaa
     4621 aataattgca cagttgtcaa cttatctagt tcaagtaatt atttccactt attgcgtgca
     4681 caagcggcta gtttagattc tgaacaaatg cgaccattgg ttgttatgtc accaaaaagc
     4741 ttactgagaa ataaaacagt tgcaaaacca attgatgaat ttacttctgg tggatttgag
     4801 ccaattttga cagaatcata tcaagcggat aaggttacaa aagttatttt ggcaactggt
     4861 aaaatgttca ttgatttaaa agaagcatta gctaaaaatc cagacgaatc agtattactc
     4921 gttgcgattg aaagattgta tccattccca gaggaagaga ttgaagcatt actagcacaa
     4981 ttgccaaacc ttgaagaagt gtcatgggta caagaagaac ctaaaaatca aggtgcatgg
     5041 ttatatgtct atccatatgt taaagtgcta gttgcagata aatatgattt aagttatcat
     5101 ggcagaattc aaagggctgc tccagctgaa ggcgatggag aaattcataa acttgttcaa
     5161 aataaaatta tagaaaatgc attaaaaaat aactaggggg aaataagtca tgccagaggt
     5221 taaagttcca gaattagcag aatctattac agaaggtacc attgcagaat ggttgaaaaa
     5281 cgtaggggat agcgtagaaa aaggtgaagc tattcttgaa ttagaaactg ataaagttaa
     5341 tgtcgaagtt gtatctgaag aagcaggtgt attatctgaa caacttgcaa gtgaaggcga
     5401 cactgtagaa gttggacaag caattgctat catcggcgaa ggtagtggca atgcttctaa
     5461 agaaaatagt aacgacaata ctccacaaca aaatgaagaa acaaataata aaaaagaaga
     5521 aacaacaaat aattcggtag ataaagctga agtaaatcaa gcaaatgatg acaatcagca
     5581 acgtattaat gctacgcctt ctgcgcgtcg atatgctcgt gaaaatggtg tgaatcttgc
     5641 tgaagtaagt ccgaaaacaa atgatgtggt tcgtaaagaa gatattgata agaaacaaca
     5701 ggcaccggca tcaacacaaa caacacaaca agcatctgca aaagaagaga aaaaatacaa
     5761 tcaatatcct acaaaaccag tgattcgtga aaaaatgtca cgtagaaaga aaacagctgc
     5821 caaaaaatta ttagaggtat ctaataatac agctatgtta acaacattta acgaagttga
     5881 catgacaaat gttatggaat tgcgtaaacg taagaaagaa caatttatga aagatcatga
     5941 tggtactaaa ttaggattta tgtcattctt tactaaagct tctgtagcag ctttgaaaaa
     6001 gtatccagaa gttaatgcag aaatcgacgg cgacgacatg attacgaaac aatattatga
     6061 tattggtgta gctgtttcta cagatgatgg attattagta ccatttgtaa gagattgtga
     6121 taaaaagaat tttgcagaaa tcgaagcaga aattgctaat ttagcagtta aagcacgaga
     6181 gaaaaaactt ggcttagatg atatggttaa tggttcattt acgattacaa atggcggtat
     6241 ttttggatca atgatgagta cgccaattat caatggtaat caagctgcaa tcttaggcat
     6301 gcattcaatt attacaagac caattgcgat tgatcaagat acaatcgaaa atcgtccaat
     6361 gatgtatatt gcattaagct atgatcatag aattattgac ggtaaagaag cagttggatt
     6421 cttaaaaaca attaaagaat taattgaaaa cccagaagac ttattattag aatcttaatc
     6481 cacaacacaa aatagtttac tattcctaaa agcgggatta aatcaataac aaacagcagt
     6541 aagattattt cctagtcgaa atatcttact gctgtacttt atttttataa tgattcgtat
     6601 taaaatgtgg gtttaatttt gactacttaa aaaatatatc atttctattg aaatagactc
     6661 acaatagaaa tataggtaga atagcgtgtt tcaatatgct aatgaatgca ttttagataa
     6721 taacaaacga tgaaatatat gaattctata agcgccttta gttaatttaa aatcatctga
     6781 acatgatgta aattcgcttt atggacacga catatatatg tttttcatgt cctacaaaaa
     6841 acgaaatatt caattgctat atacagatat tcattaatga cacactcaat agcaaccata
     6901 acaatattat gttacctatt taaatgaaat agttctttta aagaaataca tttttcacat
     6961 attaatctat aatcaaaatc aactgaccga tattctataa tttatgatta aaataagtta
     7021 taatataata gtaaagataa agatagaggt ggctataatg tgtggactta gaagtataac
     7081 attaggtaca acaaatatag aacagacaaa acatttcatg gttgacatat taggattaaa
     7141 ttatgaagaa cttcttgaaa actcaattcg tttcggcgat gcagatataa gcccaggaac
     7201 aagacttcaa tttatacaag ttccaagtga gcaattagaa gaatctcact ttgtgggtat
     7261 tggattacgt acaccaactg actcaggttt agaggagtat gcggaaatat tatcgaataa
     7321 ggatattcca tttacaacag ttaaagaatt aaatggcaat aaatatttca gtctcgaaga
     7381 taacaatggt catattttct caatatattc aaacgagaat aattatggcg ttggtttagg
     7441 tatgccttct tttgagagtg cggtcaatcc gttacatcaa gtgcaaggtt taggaccagt
     7501 gattcttaaa gtgaatcatg tagatattac aggtcaaatt ttaacaaata tattcggact
     7561 tgaagtattt gcagaatacc aacccttcga caatgctgac tatcatgtcc aagtattcaa
     7621 agttggaacg ggtggtctag gtggcgaaat acatttaatg cctgtagaaa ccgaaatgac
     7681 aatgccggaa tatggcgcag tcgatcaagt tgagtttgaa acgaaagatg cagatttctt
     7741 taatcaagcg aaatcgcgct tagatgaagt ggaaatacca tatcaaacgc ttgagcaaga
     7801 tgatattgaa tcaattagaa ttactgaaaa cagtggatta tcgtttatat tcactttaca
     7861 aaaataattt ttttacgata gcgaggacaa aatttatgtt acatgaaact tggaaagaac
     7921 gtacaccaat caagaaagta gaagtcatta atacagatgc aaagaaattt acagtttctg
     7981 acatgttaac ggttggcaaa caatatgatg ttatcaacga aactgaagaa tattatcaaa
     8041 taatagataa ttctggatta gttggcggtt actataaaac atatttcaaa gaagtttaaa
     8101 cgcataatta ttgaaatgaa gcctaatatg agattttcaa ttagtaaatt tggtaaaata
     8161 taaacaattc gcatactaag cattgcgttt tatatcagaa tagaatagta aactaaggca
     8221 tttggagaac ttattccatg tcttagtttt ttaatttatt taaaggagaa cgttttaaaa
     8281 tggcactaaa acattataag aattcagatt caacagtttt caatgatgcg aaggcattat
     8341 ttgatttaaa taaaaatatt ttacttaaag gtccaacagg ttcagggaaa acaaagttgg
     8401 cagaaacatt aagtgaagtt gttgatacac ccatgcatca agtcaattgt tctgttgatt
     8461 tagatacaga aagcttatta ggctttaaaa caattaaaac aaatgcggaa ggtcaacaag
     8521 aaattgtctt tgtagatggt ccagttatta aagctatgaa agaggggcat attttatata
     8581 ttgatgaaat aaatatggct aaacctgaaa cattgcctgt attaaatggg gtcttagatt
     8641 atcgtcgtca aattacgaat ccatacactg gtgaagtaat caaagctgta ccaggattta
     8701 acgttatagc agcgataaat gaaggttatg ttggtacttt gccaatgaat gaagcactaa
     8761 aaaatcgctt tgttgttatt cacgttgatt atattgatgg ggacatttta aaaaatgtga
     8821 ttaaagagca aagtttatta caaggtgata aacaaatcga acaaattatt aagtttaatg
     8881 aagatttacg tactatgtct aagcagggac aaatttctga agaagccgct agtatccgtg
     8941 cattattaga cttgtgtgat ttaatcactg taatgccagt tgaacgtgca attaaacgta
     9001 caattattga taaattggaa gatgaacgtg aacaacaagc aatatataat gctgtagaac
     9061 taaactttta aagagggata aaacatgagt gatcgtttca taaaatttaa tgacgaacag
     9121 ttagatgcca agcaagtaat gatgttgcaa gacttagctc gactcttatt aaaaaatgaa
     9181 caaacacaag ttaaaatcca aaaattccct tactataatc ctgttcaaaa tgttttgatt
     9241 acgagttggt tttggtcaca tcgaccaagc catatcgaaa tggctggttt aaaaacagat
     9301 gttatgttag ctgcttacgg ttatcatatg atggatgtac aaattgtaaa tgaagtagta
     9361 caagataaaa cttttaaaca ccctaaattt tatcaacaac tttttaaatt attagaagat
     9421 atgcgcgtat taaattctat taaagttgag cgcccaagta ctgctaaatt aattgatctt
     9481 cgcttagata cacgtatatc atatacggaa tctcaaatta aagtgtatcg tactaaaaca
     9541 caatatacag atttattatt tttgtattta gaacatgcat ttttaagcca agatttcttt
     9601 gatataccat ctatacattc agatttagat gatattttag taaatatgtt cttatattta
     9661 cctaattttt tccaaaatca aaattctgaa gataatatgt atctggctca aagaattatg
     9721 tatcaagtag atgatatttt aaaagaagat atgctaaacg aatattatta cttacctaaa
     9781 acactatata atacattagc tagtccagag tttgatgatt taaagagaac tgatgcaagt
     9841 caagtcgatg ggcaagatga tacttcagaa gatgatgata acgaaagtga aaaagctgac
     9901 tctaaatctg cagatagtga gtcaaaaggt ggcgcctatt tagaaatgga gcttcacgaa
     9961 ggacaaaata gtgaaacatt aggtaatgat gaagcacgtg aaggcgatgc tacagatgat
    10021 atgactgata tgatgaccaa aaagggcaaa ggatcaaacg atacgctaaa tcgtgaagaa
    10081 ggcgatgctg ttggtcaaag ccaagcattt caacttgatg gtgtgaacaa aaatgtagaa
    10141 ataaaatggc aaattcctga aattgagcca caatatgttt tagaatatca agaatcgaaa
    10201 caagacgtac aatatgaaat caaagactta attcaaatta taaagaaaac gattgaacga
    10261 gaacaacgag atgcacgttt taatttaaca aaagggcgat tacaaaagga tttaattaat
    10321 tggtttatcg acgatcaata taaattgttt tacaaaaaac aagatttaag taaatcattt
    10381 gatgcgacat ttacattgtt aattgatgcg tcagcaagta tgcatgataa aatggctgaa
    10441 acgaagaaag gtgtagtgtt attccacgag acgttaaaag cattgaacat taaacatgaa
    10501 attttatcat tcagtgagga tgcctttgat tcagacgagc atgcacaacc aaatatcatt
    10561 aatgaaatta ttaactatga ttactcaacc tttgaaaaag atggcccacg tattatggca
    10621 cttgaacctc aagatgataa tcgtgatggt gttgctatta gagttgcaag tgaacgatta
    10681 atgcgtcgaa atcaacatca acgattttta atcgtatttt cagacggcga accgtctgca
    10741 tttaattata gtcaagatgg tattattgat acgtatgaag ctgtagaaat gtcacgtaaa
    10801 tttggtattg aagtatttaa tgtatttttg agtcaagatc caataactga agatgttgaa
    10861 caaacaattc ataatattta tggtcaatat gctattttcg ttgaaggtgt tgcgcattta
    10921 cctggacatc tctctccatt att
//
LOCUS       DAKTGS010000098        10701 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.098, whole genome shotgun sequence.
ACCESSION   DAKTGS010000098 DAKTGS010000000
VERSION     DAKTGS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.098"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(1..258)
                     /gene="tnpA"
                     /locus_tag="O0286_002004"
                     /pseudo
     CDS             complement(1..258)
                     /gene="tnpA"
                     /locus_tag="O0286_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(562..816)
                     /locus_tag="O0286_002005"
     CDS             complement(562..816)
                     /locus_tag="O0286_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170372.1"
                     /translation="MLIKIRGGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDL
                     IYIFCTLLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
     gene            complement(972..1880)
                     /locus_tag="O0286_002006"
                     /pseudo
     CDS             complement(972..1880)
                     /locus_tag="O0286_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(2105..2317)
                     /locus_tag="O0286_002007"
                     /pseudo
     CDS             complement(2105..2317)
                     /locus_tag="O0286_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(2392..3927)
                     /locus_tag="O0286_002008"
     CDS             complement(2392..3927)
                     /locus_tag="O0286_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0170373.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3978..5522)
                     /locus_tag="O0286_002009"
     CDS             complement(3978..5522)
                     /locus_tag="O0286_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0170374.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            5751..6707
                     /locus_tag="O0286_002010"
     CDS             5751..6707
                     /locus_tag="O0286_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0170375.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            6921..7694
                     /gene="phnC"
                     /locus_tag="O0286_002011"
     CDS             6921..7694
                     /gene="phnC"
                     /locus_tag="O0286_002011"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCY0170376.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            7696..8496
                     /gene="phnE"
                     /locus_tag="O0286_002012"
     CDS             7696..8496
                     /gene="phnE"
                     /locus_tag="O0286_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0170377.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            8493..9308
                     /gene="phnE"
                     /locus_tag="O0286_002013"
     CDS             8493..9308
                     /gene="phnE"
                     /locus_tag="O0286_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0170378.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(9439..10617)
                     /gene="deoB"
                     /locus_tag="O0286_002014"
     CDS             complement(9439..10617)
                     /gene="deoB"
                     /locus_tag="O0286_002014"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCY0170379.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
BASE COUNT         3465 a         1948 c         1657 g         3631 t
ORIGIN      
        1 tacttttcct tttaaatagc caacaaatga tgatacccca agtttgggtg gtatactaac
       61 taacatatgg atatgatctt tacatacctc tgcttcaatt atctctacac cttttctttc
      121 atataattga cgtaatataa tccctatatc tttttttatt tttccatata tcgcttgtct
      181 tctgtattta ggtgcaaaga caatatgtta cttacaattc catttcgtat gtgctaaact
      241 gtttgtgtca gatgacatta aatagcatct cctcgtgttg attattttgg ttggctgacc
      301 aatatttact ctaacatgta gagatgcatt tttttgacaa tggtagaacc ttttctgggg
      361 agtgggacag aaatgatatt ttcgcaaaat ttatttcgcc gtcccacccc aacttgcatt
      421 gtctgtagaa attgggaatc caatttctct ttgttggggc cccgccccaa ctcgcattgc
      481 ctgtagaatt tcttttcgaa attctctgtg ttggggcccc tgactagaat tgaaaaaagc
      541 ttattacaag cgcattttcg ttcagtcaat tactgccaat ataacttcgt agatcataga
      601 agattgattt atttcccagc ctattctttt tataatattt agcaaaggta ataatgatat
      661 taataatgta caaaaaatat aaatcaaatc gacatcctta taaaacatca gaaccactaa
      721 aaacaaaaaa gcacaaaata aaattaaatt taaaataaac gaccactttt caaaaaaatc
      781 tcttttcata tttccacccc taattttaat aagcattatt ttatattctc ttttaagttt
      841 attattcaaa aggaaaacag aaatatcttt caatattatt ataaacattt caactacttt
      901 taaaaaccaa caaaaaaata cttattttaa gtagatgagc ataagtgaac atagttcttt
      961 agttataata attaattcaa ccaaaagtcg atttgttttt gcaattggtt ttcatttcct
     1021 cttaaagata ttttcattaa atctgtcaaa tcaatagacg ctatattttt caacttatct
     1081 ctatatttat ttttagtacg tctttctaaa tttccccatt cctcttcttc gtgagttaat
     1141 aaatgaagca ttgctcgttc ttgtatattt tcaatcattt ttaaattcgg ttttaaaata
     1201 tgcaaatcat caaaacaatc tttccaacaa tcaaccatat ctcgttttaa ttcaatttcc
     1261 acacgccata gaaatgttga atcaatttca acatctgcat tatctttacg ttcttgtttt
     1321 tattataaat ccgaataaac ctatcactat tacgcacacc aaaatatttt gtttctggtt
     1381 ttacattacg tccataaaat atagttttct ttaccgactt atctgacaat gcataatagt
     1441 catttaaatc aaattcaaaa tcaaaagcca aatctaatct cgtaaaacta acatcgtcca
     1501 aataactgat gatattttgt tttaaccaaa gcacttcatc atgcgaaagc ttattaggat
     1561 taaattcaac gcgcatacta cgtctattcc aaagagttgc ttttattttg tcatattcaa
     1621 tataaacttt ttctttaaga gctttagctt taaagtttgt ttgtaaaata tcccaaagcc
     1681 gaatttcagg attagtactc ataaaatgtg aaagtctctc tgcgttagac atgctaagat
     1741 tcccaacaat cgttatagcg tcaaaagaca attttggaat agctagtgac atcctatgtc
     1801 gatttaaccg gctattaccg gatattagag tatccagttt tacaaatgga tgaaacgaaa
     1861 ttcaaaacac taaaaaatat gttccactaa cagcaaaaaa ataccattat gttcctacta
     1921 aaaaactaaa aatactggag aacaaatgtc aggatataac ttaggatact atgtaataaa
     1981 aatttacaat aaaaaaacag gaaaacaaat ttcaagtaaa agaataccca tacaaagagg
     2041 ataaaataaa aaacctcgaa ctgaaatgat gatcttttca gctcgaggtt taaatattgg
     2101 tgccttattt atatagattc gttatattat attctctatt ttcattaaca taatccttaa
     2161 agagttttaa attaatacct gctagatgat tcaaaaatgt ttcatcaact tttaaataat
     2221 tcaataattt ttgtggtgtc agtaaatttc tatcaaaata caactttaat aaactattca
     2281 ttttgacagg acgtgacatt tcaatcacgt cgtctaaaga taatactttc tcgctttaaa
     2341 caaaaacaaa aacttacccg attaaaatca agtaagtttt acatatatcc gtcacttttc
     2401 aactttaaaa tcaacaactt gcgggatgcg catcaaatta ttatttgata aaaaatcaat
     2461 aagtaattgt gcgccttcaa cttgaatatc ttttacaact ggcgcgtcga tatacatatc
     2521 atactgacca ccgcctactg cacgataatt atttacacaa attgtatatg tctgctttaa
     2581 atcaactgcg tgaccttgaa tcatcatatt gctcacacgt tgtccctttg gtcttccaac
     2641 atgaatggta taacttacgc caccatatat atcataatta aagtgttgtg gtttgggttc
     2701 aaggaagtct gcgctcacac taacttcatc atttttcacg tcaaaatatt ctgctgatcg
     2761 ttcaatggct tctttaagtt tggcaccact tacagctaaa actttaaatg tatttggaaa
     2821 tgggtaattg ttaataacat ctcgcatcgt cacgacttgc ttgaaaccac tagcagaatc
     2881 aaacaaagct gtacaggcaa catctgcgtc acttttttct aataaagcgt aattcataaa
     2941 atttgtaaaa ggatgcggtg ccacacgtgc ctcaaatgca tgattaatcg tcatatcata
     3001 tggcaatgta gtaatttcgt aatctaacca gtcctctaac tgctttcgta aatgttggtc
     3061 atcttcatca atagtaaatg tggaatcatc tataacagga agtaattcac atgattcaac
     3121 ggatagattt tcatattcat cagtactcaa gactactctg cctacagttg tacctctcgt
     3181 accaggttga atcacagccg tttgcttaaa cctttcagca atttgtcgat gttggtgacc
     3241 cgtaataaag atatctatat ctttagaaaa cgcttctaac atggcatatc cttcattttc
     3301 acccgttaat acttcggtcg gcgtaccact ttctaaatcc ttttcaaatc caccatggta
     3361 acaaaccaca atgatatctg catgtcgctt catttcaggt aagtattgtt gaagtatttc
     3421 aaaagcacta tgaaacgtaa gtgactgaat atgctctggt tgttcccaat ggggaataaa
     3481 ttgtgtcgtt aaacctatca caccaacagt ttgatctcca acctgaaaat acttcacacc
     3541 gttatcagtc aatgtactat cattttcata tatattagcg cacaaaactg gataattgag
     3601 tctgcgtaaa gtgtctttta agtatggtaa tccataatta aattcatgat taccaagcgt
     3661 accaaagtcg aatgccattc gattataaaa atcaactaaa ggctggctac tgccgctatg
     3721 cgcgattaag taattacaaa atggtgaccc ttgcaaaaaa tcaccattat ctattttaaa
     3781 actttggtca tactgccttc tgtcttgttc tataacatga ttcgctagta acaatcccat
     3841 aggttgatat tgatttctac tcgtaaaatc tgttgggaaa atataaccat gtacgtcact
     3901 cacgacataa aatgctatgt ttgacatcct cactcactcc ttcaatcaca aacatctttc
     3961 ttatttctat tatatattta tttgaagtct gttgtaatca aggttttgtc accgagtttt
     4021 aaacgaatct ttgaaccttc catactttca agtactttag cattgacctt aattgtgaca
     4081 tttccgtttt catctgcttt aactgttggc aaagtactgt aacctggtgg gttataatcg
     4141 ttatctttac ttgaaaattg tccgatttga cgtccgcctt ctttgtaaat accaactcta
     4201 agattttcaa gtgtttgatt tgcttcaaat ccttttaaat gtattgtcat ttcaaatggc
     4261 tcatttacag aaacattttg cgggatatca aatgttactt tttcgttctg atttggtggt
     4321 gtatgatcat ctggtgtgtt tggctgagga tctgcgcctt tttcgctgcc ataactacct
     4381 gctttaaatg ttgttggatc ataccattta taaccactcg gcggttgtga ccatggctct
     4441 ttttcaggct cagttgaacg ctctggtcgt tcaaaatcaa gcaacttagt ctttgtatct
     4501 aatgttaggc tactcgcctt aagtgatttc ccatcattat ctttagacat ccaagccgtt
     4561 atattattta atagcttacc gttgtcttgt tctttaaaac catcatatgt tttcttcttt
     4621 tctccattat cttctcttac atatttgggc gaactatctt ccacaagtga tgaatcaccg
     4681 ataaatgctg ctttaccttt tccaacttta gaaattgcta cataggggcc ttctgcttta
     4741 ccgcccccat tataaatacc ttgatctaca gcatgtgacc atttactttt cgctggcaat
     4801 tgttctggtg tatacacaat accttttgct ttctctggat tagtaattgc taatgtcgat
     4861 ccggcatgca tagagacaga tttcacacct tcagtaatac cgaaactttc ttttgaagaa
     4921 acaatattgc tcgtatttaa atcacctagt gcattatatc gaaaacgtac gccaaagttt
     4981 gtagataacc aatctgaact tttcacacct tgcattgcag tagaactttt ttcttctgca
     5041 ttcatacctt tcgacatatc ttcatatgct ccacgtcgat aaccattcat tgcctccgat
     5101 gaatcaatac gatttaaatt tcggtcagca ttgtaatgat ctgaaataaa gacaacattg
     5161 ccaccttgtt tcacatattt aacaattgct gcctgttctg attctttgaa aggaatgtta
     5221 gcctcaggaa ttacaaatat tttggaactt ttcaaacttg cttctgttat gttcgaatga
     5281 ccatcaatag ctttaacgtc ataaccttgt ttttgtattg aatccgcata atctgaaaat
     5341 gcaccatcac taacccaatc tgcagcacca gctgtttgac catgagaacg atcgaataat
     5401 accgttcgct gttgctttgt aggttgcgat tcatgcgtta tagctaaaga ttgcggtaaa
     5461 gcacttaatg ataccgttgc aacaattgca gagacagtta atgacttata tatttttttc
     5521 attttgtgag gctcctttta aaataaattt gttcttgaat tataggataa aaattcgttg
     5581 catatgagca atttaacgaa aaatttacaa aatcttatca aactcttaaa gaaagttatt
     5641 aaaattcatt tttataaaat actttttaac atttaaatgt ggtacgctat aagtgtaatt
     5701 tcattgcata catattacac gattaagaat gtgaagggga cagttatcaa atgaaaaatt
     5761 ttaagtgttt atttgtatta atgttagcag tcattgtttt tgcagcagca tgtggaaact
     5821 caagttcttt agataatcaa aagaacgcta gtaatgattc ggattctaaa tcaggaggat
     5881 acaaacctaa agaattaacc gttcaatttg taccttcgca aaatgctgga acattagaag
     5941 ctaaagcaaa accattagaa aaattactat ctaaagaatt agggattcca gttaaagtgt
     6001 ctgtatcaac taactacaat acaattgttg aagctatgaa gtctaaaaaa gttgatgttg
     6061 gtttcttacc accaacggca tacacattag cacatgatca aaaagcagct gatttattat
     6121 tacaagcaca acgtttcggt gtaaaagaag atggttcagc aagtaaagaa cttgtagata
     6181 gttataaatc agaaattctt gttaaaaaag actcaaaaat taaaagcttg aaagatttaa
     6241 aaggtaagaa aattgcctta caagatgtaa catcaactgc tggatataca ttcccacttg
     6301 cgatgttaaa aaacgaagca ggtattaatg caactaaaga tatgaaaatt gtgaatgtta
     6361 aaggtcatga ccaagcagtt atctcattat taaatggaga tgtagatgct gcggctgtat
     6421 ttaacgatgc acgtaatact gtgaaaaaag accaaccaaa tgtatttaaa gacacacgaa
     6481 ttttaaaatt aacacaagct attccgaatg acacaatttc tgtaagacca gatatggata
     6541 aagattttca agaaaaattg aaaaaagctt ttatagacat tgctaaatca aaagaaggtc
     6601 acaaaattat tagcgaagtt tattcacatg aaggatacac agaaacgaaa gattcaaatt
     6661 tcgacattgt aagagagtac gaaaaattag ttaaagatat gaaataatca ttatttaaca
     6721 aatgaatcat tagcgaattt ggtattaaaa gctttcgttc aatagatata ttctagatta
     6781 atattgaaaa gctaggcgct aaactgaaac agatatagaa aggtgtcgct gtacatttga
     6841 aaccatttgt acacagaaac ccaatgtcta tgatatttca gtttaccttg gcttttcttt
     6901 attaaagaaa ggtgtcaaac atgagtcaaa tcgaatttaa aaacgtcagt aaagtctatc
     6961 ctaacggtca tgtaggcttg aaaaatatta acttaaatat tgaaaaaggt gaatttgcag
     7021 ttattgtcgg actatctggt gctgggaaat ccacgttatt aagatctgta aatcgtttgc
     7081 atgatatcac gtcaggtgaa attttcatcc aaggtaaatc aatcactaaa gcccatggta
     7141 aagcattatt agaaatgcgc cgaaatatag gtatgatttt ccaacatttt aatttagtta
     7201 aacggtcaag tgtattacga aatgtactaa gtggacgtgt aggttatcac cctacttgga
     7261 aaatggtatt aggtttattc ccaaaagaag acaaaattaa ggcaatggat gcactagaac
     7321 gcgtcaatat cttagataaa tataatcaac gctctgatga attatcaggt ggccaacaac
     7381 aacgtatatc tattgcacgt gcgctatgcc aagaatctga aattattctt gcagatgaac
     7441 cagttgcttc attagaccca ttaactacga aacaggttat ggatgattta agaaaaatca
     7501 accaagaatt aggcatcaca attttaatta atttacattt tgttgacttg gcaaaagaat
     7561 atggcacacg catcattggt ttacgtgatg gtgaagttgt ctatgatggt cctgcatctg
     7621 aagcaacaga tgacgtattt agtgaaatat atggacgtac aattaaagaa gatgaaaagc
     7681 taggagtgaa ctaacatgcc tttagaaata cctacaaagt atgactccct tttaaagaaa
     7741 aaggtttctt taaaaacgag ttttaccttc atgttaatca ttgtgcttat catttggagc
     7801 ttcatttata cgggatttaa ttttggagat ttaatgatag gaatacctca aataggtgat
     7861 ctattcaaac aaatgattcc acctgatttc gagtatttac aacaaattac aacgccaatg
     7921 ttagatacca ttcgaatggc tatcgtaagt acagtattag gtagcatcgt ttcaatacca
     7981 attgcgttat tatgtgctag caatatcgtt catcaaaagt ggatttcaat accctcgcgc
     8041 tttattttaa atatagttcg tactattcca gatttgttat tagcagcaat ctttgtggct
     8101 gtatttggaa tcggtcaaat tccagggata ttagcactgt ttattttaac tatctgtatt
     8161 attggaaaat tattatatga atcattggaa acgatagatc caggtccaat ggaagcaatg
     8221 acggctgttg gcgctaataa aataaaatgg attgttttcg gtgttgtacc acaagccata
     8281 tcgtcattta tgtcatacgt attatatgca tttgaagtaa atatacgtgc ttcagctgtg
     8341 cttggattag tcggcgctgg cggtattgga ttgttttatg atcaaacact tggtttattt
     8401 caatatccaa aaacagcaac gattatttta tttactttag ttatcgtcgt cgtcattgat
     8461 tacatcagta cgaaagtgag ggcacatctc gcatgacaca ggaaatagca aaatataatg
     8521 ttcacacaaa agcacacaaa cgaaaattga ttaaaagatg gcttattgca attgtcgtct
     8581 tagctattat catctgggca tttgcaggtg taccaagttt agaacttaaa agtaaatcat
     8641 tagaaatctt aaaatccata ttcagcggat tattccatcc tgatatcagc tatatctata
     8701 taccagatgg cgaagactta ttacgtggtt tacttgaaac ctttgcgata gccgttgtag
     8761 gtactttcat cgccgcaatt atctgtattc cattagcatt tctaggtgca aataatatgg
     8821 taaagctacg cccagtttca ggtgttagca aatttatttt aagtgttata cgtgtcttcc
     8881 cagaaattgt aatggcactt atatttatca aagctgttgg cccaggttca ttttcaggtg
     8941 tattagcttt aggtatccat tccgtaggta tgcttgggaa acttttagct gaagatattg
     9001 aaggtctaga tttcagtgct gtagaatcat taaaggccag tggtgcgaat aagattaaaa
     9061 cactcgtatt tgcagtcata ccacaaatta tgcctgcctt tctatcactc atactttatc
     9121 gctttgaact aaacttacgt tcagcttcta tactggggct aattggggct ggtggtatcg
     9181 ggacaccact catatttgcc attcaaacac gttcttggga ccgtgtaggt attatattaa
     9241 tcggtttagt actaatggtc gcaattgtcg atttaatttc cggttcaatc cgaaaacgta
     9301 ttgtttaaca ttaaatcagg atactcctaa ataagaagtc ctaccgtctt acgtttctct
     9361 attataataa aaacagcagt gaagaaaact attgttatag ttaacttcac tgctgttttt
     9421 ataatatcta aatttattct atttcaattc ctttaaataa cttttaccga actctggtaa
     9481 tgttacgttg aaattatctg ctatagttgc accgatagaa ctgaatgtag tatcactttc
     9541 tagtgcatga ccacctttaa atttcggact gtacataatt actgggatat attctctcgt
     9601 atggtccgta cctggcgctg tcgggtcatt accatggtct gctgtaataa ttactaaatc
     9661 gtcttctttt aagttgctaa acagttctgg caagcgatca tcgaaatctt taattgcttg
     9721 tgcataacct ggtttatcac gacgatgacc gtataatgca tcaaagtcta ctaagtttaa
     9781 gaagctaata cctgtgaaat ctttcttaac aattttcatc aattgatcca taccgtccat
     9841 gttactcttc gtacgaaccg cttctgttac accttcacca tcataaatgt cattaatttt
     9901 accgatggca ataacatcat aaccaccgtc tttcaaatga tctaagacag ttttaccaaa
     9961 aggttttaac gcatagtcat gtcgattaga tgtacgtgta aagtttcctg gttcaccaac
    10021 atatggacgt gcgataatac gaccaattaa atatttaggg tcttttgtca actcacgaac
    10081 cttttcacaa atatcatata actcttctaa tgggataatg tcttcatgtg cagcaatttg
    10141 caatactggg tctgcacttg tataaacaat taagtcacca gttttcattt ggtgctcgcc
    10201 ccactcatcg ataatttgcg tacccgatgc cggtttgtta gcaacaactt tacgacctgt
    10261 catttcttca atttgttgaa ttaactcttc agggaatcca ttagggtata ctttaaaagg
    10321 ttgcataata tttaatccca taatttccca gtgaccagtc attgtatctt taccaactga
    10381 agcttcactc aatttagtat agtatgcttc tggttgttca actgcattta ctactggtaa
    10441 tttatcgatg ttccctagac ctaacttttc aaggtttggt aaagtttgat cgaaaccttc
    10501 taaggtatgt cttaaagtat gtgaaccttc atctttaaaa tcagctgcgt ctggcgcttc
    10561 accaatacct actgaatcca ttacgattaa atgtacacga ttaaatggtc ttgtcatagc
    10621 tatcactccc aaaatttata tatattagta atctgaatct gcttctaaac cttgcataat
    10681 ttgaacacct gcgctcgcac c
//
LOCUS       DAKTGS010000099        10661 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.099, whole genome shotgun sequence.
ACCESSION   DAKTGS010000099 DAKTGS010000000
VERSION     DAKTGS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.099"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            68..1360
                     /gene="obgE"
                     /locus_tag="O0286_002015"
     CDS             68..1360
                     /gene="obgE"
                     /locus_tag="O0286_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCY0170380.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            1373..1828
                     /locus_tag="O0286_002016"
     CDS             1373..1828
                     /locus_tag="O0286_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0170381.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            1842..2444
                     /gene="ruvA"
                     /locus_tag="O0286_002017"
     CDS             1842..2444
                     /gene="ruvA"
                     /locus_tag="O0286_002017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCY0170382.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            2476..3480
                     /gene="ruvB"
                     /locus_tag="O0286_002018"
     CDS             2476..3480
                     /gene="ruvB"
                     /locus_tag="O0286_002018"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCY0170383.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            3482..4507
                     /gene="queA"
                     /locus_tag="O0286_002019"
     CDS             3482..4507
                     /gene="queA"
                     /locus_tag="O0286_002019"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCY0170384.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            4530..5669
                     /gene="tgt"
                     /locus_tag="O0286_002020"
     CDS             4530..5669
                     /gene="tgt"
                     /locus_tag="O0286_002020"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCY0170385.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            5688..5948
                     /gene="yajC"
                     /locus_tag="O0286_002021"
     CDS             5688..5948
                     /gene="yajC"
                     /locus_tag="O0286_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCY0170386.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            6223..8502
                     /gene="secDF"
                     /locus_tag="O0286_002022"
     CDS             6223..8502
                     /gene="secDF"
                     /locus_tag="O0286_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCY0170387.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            8705..>10661
                     /gene="recJ"
                     /locus_tag="O0286_002023"
     CDS             8705..>10661
                     /gene="recJ"
                     /locus_tag="O0286_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCY0170388.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIP"
BASE COUNT         3799 a         1480 c         2091 g         3291 t
ORIGIN      
        1 tataaggggc tctaatatgc tataattatt gggaaaatga aaattatatg taaaagaggt
       61 gagatatatg tttgtcgatc aagtcaaaat atctcttaaa gccggtgatg gtggtaatgg
      121 tattaccgca tacagaagag aaaaatatgt accatttggt ggaccagctg gcggtgacgg
      181 tggtaaaggt gcttcagtcg tatttgaagt ggatgaaggt ttaagaacgt tattagattt
      241 tagatatcaa cgtcatttta aagcaagcaa aggtgaaaat ggccaaagta gtaatatgca
      301 tggtaaaaat gcggaagatt tagtattaaa agttccacct ggtacaatta ttaaaaatgt
      361 tgaaacagac gaagtgttag cagatcttgt tgaagatggt caaagagctg tagtagcgaa
      421 gggcggtcga ggtggccgag gtaattcacg ttttgcaaca cctagaaacc ctgcacctga
      481 cttcagtgaa aaaggtgaac caggtgagga attagatgta tctttagaat tgaaattatt
      541 agctgatgta ggattagtag gtttccctag tgtgggtaaa tcgactttat tatctatcgt
      601 ttcaaaagct aagcctaaaa ttggggcata tcattttaca acgattaaac caaatctagg
      661 tgttgtttca acgcctgatc aacgtagttt tgttatggca gatttaccag gtttaattga
      721 aggtgcatct gatggcgttg gattaggaca tcaattttta agacatgtag agagaacaaa
      781 agttattgtt cacatgattg atatgagcgg ttctgaaggt agagaaccta ttgaagatta
      841 taaagtcatt aatcaagaat tagctgcgta cgagcaacgt ttagaagata gacctcaaat
      901 cgtagtagct aacaagatgg atttacctga atcacaagat aatttaaact tgtttaaaga
      961 agaaattggc gaagatgtgc cagttattcc agtttcaaca ataacgcgtg ataatattga
     1021 tcaattatta tatgcaatag cagataaatt agaagaatat aaagatgttg acttcacagt
     1081 tgaagaagag gagtcagttg gcattaaccg agtattatat aaacatacac cgtcacaaga
     1141 taaatttaca atttcaagag atgatgatgg tgcttatgtg gtaagtggta atgctattga
     1201 aagaatgttt aaaatgactg actttaacag tgatccagca gtacgtcgat ttgctcgtca
     1261 aatgcgttcg atgggtattg atgatgcgct tagagaacgt ggttgtaaaa atggtgatat
     1321 cgttagaatt cttggcggag aatttgaatt cgttgaatag gagcgaaaca tgatggacaa
     1381 taaagattat aaaaagtttt atttaattag agaagatgtc ttgcctgaat ccgtggttaa
     1441 aacattgaag attaaagatg ccttaaaaag tgatccgaca ttgtccattt atgatgccgt
     1501 taaacagttt gatctatcta gaagtgcttt ttataaatat agagaaacga tatttccagt
     1561 agacgataaa atgcttgacc atcgagaatt tacattaatt ttatatgtaa ctgatattgt
     1621 tggtatgttg gcacgtgtac tagatgttat atcaaagtta gaactatctg tattaacgat
     1681 tcatcaaagt attccaatgg aagaaaaagc aacaataaca ttatcactga atgctaaatc
     1741 taaagaaact tcagtagaag atgttattgg cgctttgaga aatttagatt atgtatcaaa
     1801 agtagaatta attagtatga gtatgtaagg atgtgcctat aatgtacgcg tatgtcaaag
     1861 gtaagttaac acatttatat cctacacacg tagttgttga aactgctggt gttggttatg
     1921 aaattcaaac accaaattct tatcgttttc aaaagcatct agatcatgaa gttttaattc
     1981 atacatcttt aattgttcgt gaagatgcac aattattgta tggatttagt agtgaagaag
     2041 agaaagatat gttcttgagt ttaattaaag ttactggtat tggtccgaaa tcagctttag
     2101 ctattttagc gacaagtacg cctaatgaag taaaacgtgc cattgaaaat gaaaatgata
     2161 cgtatttaac taaattccca ggaattggta agaaaacggc aagacagatt gtcttagatt
     2221 taaaaggtaa agtgaaaatt actgaagaag atagcgattc attattacaa gtagacgcta
     2281 cttcgacggt gcaagatcaa ttcgtgcaag aagcaatgtt agcgttagaa gcattaggtt
     2341 attctaaacg agagcttgca aaagttgaga aaacgttaaa taaaaataaa tatgactcag
     2401 ttgatgaagc tgttaaggca ggtcttcaat tagttgtatc ttaattttaa atagattaat
     2461 aggggaagtg ttgtcatgaa tgagcgtatg gttgatcaat caatgcatag tgaagaaact
     2521 gatttcgaat tgtcgcttag acctacgaga ttacgacaat atattggtca aaattcaata
     2581 aaaagtaatt tagaagtatt tattaaagcg gctaaacttc gtcatgaacc attagatcat
     2641 gtattgcttt ttggcccccc tggattaggt aagacaacat tatctaatat cattgccaat
     2701 gaaatggaag ttaatatacg tacagtatca gggccttcat tagaaagacc tggtgatttg
     2761 gctgcaattt tatcaggact tcaacctgga gatgttttgt ttattgatga aatacacaga
     2821 ctgagtagtg ttgttgaaga agtgttatac cctgcaatgg aagatttctt tttagatatt
     2881 atcattggta aaggcgatga ggctagaagt atccgtatcg acttacctcc attcactttg
     2941 gtaggtgcaa caacgcgagc tggcagctta acaggtccac taagggatcg atttggtgtg
     3001 cacttaagat tagaatatta taacgaatca gatttaaaag aaatcattat tagaacagct
     3061 gaggttttag gcacaggtat tgatgaagaa agtgccattg aacttgctaa acgttctaga
     3121 gggactccaa gagtagcaaa tcgactattg aagcgggtaa gagacttcca gcaagtgaat
     3181 gaagatgaac aaatatacat tgaaacaacg aagcacgcat taggtttact tcaagttgat
     3241 caacacggac tagattacat tgatcataaa atgatgaact gtattattaa gcagtataat
     3301 ggcggacctg ttggtttaga tacgattgcc gtaacaattg gtgaagaacg tattacaatt
     3361 gaggacgttt atgagccatt tcttattcag aaaggctttt tagaacgtac gccacgtggc
     3421 agaaaagcaa caccattagc ttatgaacat tttgcaaagt cgaatgagga gagagaataa
     3481 cgtgaatatt gaagaatttg actatgactt accagaatca ttaattgctc aaacgccttt
     3541 aaaagatcgt gatcatagtc gtttattagt catggataga gaaactggtg aaatgaaaca
     3601 tttacatttc aaagatatca ttgagtattt tagacctggt gatacattag tgcttaacga
     3661 tacgcgagta atgccagcta gactttttgg tttaaaagaa gaaactggtg caaaagttga
     3721 aatgttaatg ttaactcaaa ttgaaggtaa tgattgggaa gtcttactga aaccagctaa
     3781 gcgtattaaa gttggtaata aattgaattt tggtaatggc aaaattatag ctgaatgcat
     3841 aaaagaaatg gatcaaggtg gacgcatcat gcgtttacat tatgaaggta ttttacaaga
     3901 aagattagat gaattagggg aaatgccact gccaccatac atcaaagaac gtttagatga
     3961 tccagatcgt tatcaaacag tttacgctaa agaaagtggt tcagcggcag caccaacagc
     4021 aggattacat tttactgatg agttattaat tgaaattaaa aataaaggtg ttaatatcgc
     4081 atttgttaca ttacatgttg ggttaggtac gtttagaccg gtgagcgtag acgatgtgaa
     4141 tgaccacgaa atgcatagtg aatattatca aatgacacaa gaaacagctg atttattaaa
     4201 tgatactaag tccaaaggac atcgcattat atcagttggt acaacttcaa cacgtacact
     4261 tgaaacaatt cgacgcgatc atgataaatt tgttgaaacg agtggctgga ctaatatatt
     4321 tatttatcca ggatttgatt ttaaagcaat tgatggccag attactaatt ttcatttacc
     4381 aaaatcaaca ttagttatgc tagtatcagc gtttagtagt cgtgaaaatg ttctgaatgc
     4441 ttataaaacg gcagtaaatt tagaatatag attctttagt tttggcgatg caatgttaat
     4501 tatataaaaa gaatgtgagg attttgaata tgcctgcagt aacatacgaa cacattaaaa
     4561 cttgtaaaca atcaggtgcg cgtttaggta tcgtgcacac accacacggt tcatttgaaa
     4621 cacctatgtt tatgccagtt ggtactaaag caaccgttaa aacaatgagt ccagaagagt
     4681 taagacaaat tgaagcaaaa atcattttgg gcaacacata tcatttgtgg ttacaacccg
     4741 gaaatgatat tatcaaacac gctgggggat tacataaatt catgaattgg gatggtccga
     4801 ttcttacaga ttcaggcggt ttccaagtgt ttagtttaag taatttacgt aaaattacag
     4861 aagaaggcgt ggaatttaga catcatacta atgggtctaa attatttttg agtcctgaga
     4921 aatcaatgca aattcaaaat gatttaggat ctgatattat gatggcattt gatgaatgtc
     4981 caccgatgcc tgctgaatat gattatgtaa aaaaatctat tgaacgtaca acacgttggg
     5041 cgaaaagatg tctagatgca caccaaagac ctgaagatca agcattgttc ggcattatac
     5101 aaggtggcga atatgaagat ttaagagaac aaagtgcaaa ggatttagta gaattagatt
     5161 ttcctggtta tgcaatcggc ggtttgtcag ttggtgaacc taaaccggtt atgtataaaa
     5221 tggttgaaca tacagagcag tttatgccta aagataaacc aagatattta atgggtgtag
     5281 gatctccaga tgcgttaatc gaatgtagta ttcgcggcat ggatatgttt gattgtgtct
     5341 taccgacacg tattgccaga aatggtactt gtatgacatc gcaaggtcgt ttagttatta
     5401 aaaatgcaaa atttgcagat gatttaagac cgttagatga gaattgtgac tgttatacat
     5461 gtcaaaacta ttcaagagcg tatatacgtc atttaatcaa ggcagaggaa acttttggta
     5521 ttcgtcttac tactattcat aatttacatt ttctgctaaa attaatggaa gatataagac
     5581 aagccattcg agaagatcgt cttttagatt tcaaagaaga attcttcgag caatatggat
     5641 taaatgttga gaacccaaaa aacttttaag caagaggagc gtataaaatg caattttcat
     5701 tactaatata tatagtcgta atttttgcgg ttatgtattt cttgatgatc agaccacaac
     5761 aaaaacgtgc gaaacagcat cgtgagttga ttaataacat tcaatctggt caaagaatta
     5821 caactattgg tggtattaaa ggtactgtta aagcagtaga tgaaacaact gttgttatta
     5881 cagttaatgg tcatggtact gaattaactt tcgaaaaacc tgctattaaa caagttgacc
     5941 cttcataatg agaagtggta ttaaaaagga tgaaacgaat agcgtttcat cctttttaag
     6001 ttataagtag tatgtagaat agcttatttt tattccaaaa aaagaaattg tgatacataa
     6061 taattgttaa atttaaatta aatatatcta tgactatttt tactccctgc tctagtatgt
     6121 taatatgtat aaggctaata aattttgtaa gaaagattta acttgcgtta taaaatagta
     6181 ttcaagtatt ctaaatacat acgtaaataa tgaggtgttc atgtgaagaa aagtagtaga
     6241 ataattgcgt tcttgctttt agtcgtattg ttgtttgccg gaatggctgc aacttataaa
     6301 agtgttataa agaatgtcaa tttaggactt gatttgcaag gtggatttga ggtgctttat
     6361 caagtcgatc ctttaaataa aggcgacaaa atagataaaa aagccttaca atcaacagcg
     6421 caaacattag aaaatcgtgt taacgtttta ggtgtttcgg aacctaaaat tcaagttgaa
     6481 gaacctaata gaattagagt acaacttgct ggtgtaacag accagaatga agcacgtaaa
     6541 attttatctt ctcaagctaa tttaacaatt cgcgatgcag aagataaagt aaaattaagc
     6601 ggttctgata ttaaacaggg atctgccaaa caagaattta aacaggaaac aaaccagcct
     6661 acagttactt ttaaagtaaa agacaaaaac aaatttaaaa aagtaacgga agaaatctct
     6721 aagaaacgtg ataatgtcat ggttgtttgg ttagatttca aaaaaggtga cagctataaa
     6781 aaagaagcac aaaagaaaaa ccctaaattt atttctgcag caagtgttga tcaaccaatc
     6841 aattctgata gcgtagaaat ctcgggtgga tttaaaggac aagaaggcgt taaaaaagca
     6901 aaacaaatcg ccgaattgtt aaatgcaggt tctttaccag ttgacttgaa agaaatttat
     6961 tcaaactcag taggtgccca atttggtcaa gatgcattag ataaaactgt gtttgcatcg
     7021 tttattggtg tagcattaat ttatttattc atgcttggat tctaccgatt acctggttta
     7081 gtagcgatta ttgcattgac aacttatatc tatctaacgt tagtagcatt taatttcatt
     7141 tccggggttc taactttacc aggattagcg gcgttggtat taggtgtagg tatggctgta
     7201 gatgccaata ttatcatgta tgagcgtatt aaggatgaac ttcgaatagg tagaacgata
     7261 aagcaagcct tttctaaagc aaacaaaagt tcattcctaa caatttttga ttctaactta
     7321 acaacagtta tcgccgcagc agtattattc ttcttcggtg aaagttcagt taaaggtttc
     7381 gcgacaatgt tattattagg tattctaatg atctttgtta cagccgtgtt cttatcaaga
     7441 ttcttattat cattacttgt ttcatcaaat atattcaaaa atcaattttg gttatttggt
     7501 gttaaaaaga ataaacgaca tgatattaat gaaggtgtag atgttcatga ccttaaaact
     7561 tcattcgaga aatggaattt tgttaaatta gctaagccat taattggagt aagtatttta
     7621 attgtagttg tcggtttagt tattctttat atcttcaagt taaacttagg aattgatttc
     7681 tcaagtggta cacgcgtaga tttccaatct aaacaagcaa ttactcaaca aaaggtagag
     7741 caagttgtaa aagatagtgg acttaaagcg gatcaaattc agatcaatgg taaagacaat
     7801 aaagtagcaa ctgttcaatt taaagacgat ttaacgcgtg ctcaagataa taaattgagt
     7861 gataatatta aatcgaaatt cggtgacaca cctcaaatta acactgtatc accaattatt
     7921 ggacaggaat tagcaaaaaa tgcaatgctt gcattaatct atgcatcgat aggtatcatc
     7981 atctatgtat cattacgatt tgaatggcgc atgggtcttt catctgtatt ggcattatta
     8041 catgatgtat tcattatagt agcgattttc agtttattta gaattgaagt agatttaaca
     8101 tttatcgccg ctgtattaac aattgtcggt tattcaatta atgatacaat cgtaacgttt
     8161 gaccgtgtac gtgaaaactt acaaaaggtt aaagtgatta cgacaacaga acaaattgat
     8221 gatatcgtta atagatcaat tagacagaca atgacacgtt caattaatac agtattaaca
     8281 gttattgtag tagtagttgc tatactattc ttcggtgctc ctacgatatt caactttact
     8341 ttagcattat ttatcggatt gatttctggt gtattctctt caatcttcat tgccgttccg
     8401 ctatggggaa taatgaaaaa acgtcagttg aaaaaatcgc cgaaacacaa attagttgta
     8461 tataaagaaa agaaatcgaa cgatgaaaag attttagttt aaaatgaatt aagcggtatg
     8521 tgaaacaata aagagttcac ataccgcttt ttactttatt taaatttaac aaaatatata
     8581 taaataagtt cccgcattca tttttaaaaa atggtataat taaagtcttg attatttaga
     8641 taagtgcttt ttatttaaag taattttaga tataatgtct tttggaaatg aggcgtaaat
     8701 atatatgatt aaaccgaagt ataagtggaa attaactaaa ccagctgaat acataagtga
     8761 tgaattaaca tcaaaattaa agttaacgcc aatcgtaaaa aaaatattag aaagtaaatc
     8821 gattattgat gaacaagcaa tagaatccat catttcagat acagatatca atcatgatgc
     8881 actgcaattg agcgatatga ctaagactat tgaaagaatt aaaagagcga tagcgaatga
     8941 tgagaaaatc ctagtttatg gtgattacga tgccgacggt gttaccgcaa ctacaatttt
     9001 agtgataaca ttacaacttt taggtgcaca agtaggatgg catattccta accgctttac
     9061 cgaaggatat ggaccaaacg aactagcatt tagaaatgct catgatgaag gcataacatt
     9121 aatcataact gttgataatg gcatacaagg tcataatgaa attaaaatgg ttcaagattt
     9181 aggagttgat gtcattgtaa ctgatcatca tgaaattggt agtacattac cagaagcata
     9241 tgcaatcgta catccaatgc atccatcgtt taattatccg ttccaacaat tgtgtggtgc
     9301 aggtgttgca tacaaattag cacaagcatt gattgaaaat gtacctgatt attttaaagc
     9361 attagttgcg ataggtacca ttgcggattt agtatcctta actgatgaaa atcgatcatt
     9421 agtgaaacaa ggattaaaag tcttaaatga tcaatgtccg acctctgtta aagcattgct
     9481 aaaagaagcg ggatacaatg acaatattga tgaagaaaca attggtttta ttattggacc
     9541 tagacttaat gctgtaggtc gtttagatga cgcatcatta gcttgcgaat tgttgatgac
     9601 tgacgtcgaa gaagaagctg catttttagc agaacaagtt gaacatttta accgtgaaag
     9661 aaaagatatt gtagcaacta tcacagaaga agcaatggct atggcagaaa cgaaagttaa
     9721 aaagggagat ttatttttac ttttagcaaa agaaaattgg catgaaggtg tcttaggtat
     9781 tgttgcttcg aaaattgtcg aaacatttgc tttgccaaca ttaattttaa atattgatag
     9841 ggagcaaaat catgcaaaag gctccgccag atcaattgat caagtgtcga tgtttgaaat
     9901 actatcagca catcaagagt taatcgctaa atttggcggc catcatatgg ctgcaggtat
     9961 gacgatggat attgaaaata tagaatcgtt agctgaaggt ttaaataaat ggatgaaaga
    10021 attatctaaa acaacttcgt tagatcccgt aaagcaagta gatgtattat taacagaaaa
    10081 tgatataacg attaaaaata ttcgtgatat gaatcgttta agacccttcg gaacagattt
    10141 ttctagacct atttttgaaa tggatgattt atcagtttct tcagttaagg cgataggtca
    10201 acaaaaaaat catctaaaat tgacacttgg tgaaagtaat atcgccgcgc tattttggca
    10261 aaatggacac ttagaacctg agttacaaga tgaacagcca attaatatat taggatctgt
    10321 tcaaatcaac gaatggaatg gtaatcaatc accgcaaata attattcaag atattgcgat
    10381 gaatgaacag caaatattag attatagaag taagcgaaaa agtttacctt ttacagaaaa
    10441 tgatgaaaat attgtcgtgc ttattcatcc taaaagtgat aaagtaaatg cgaatgaata
    10501 ttattatggt gaagaaatta aacaacaaac tgataaagta gtattaagag atttaccaac
    10561 gtcaatggaa gacttgtcta attccttgca acaactgcaa ttttctcaac tttatatagt
    10621 tttgcaacat aatcattcga tttacttcga tggtatacct a
//
LOCUS       DAKTGS010000100        10575 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.100, whole genome shotgun sequence.
ACCESSION   DAKTGS010000100 DAKTGS010000000
VERSION     DAKTGS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.100"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            45..1982
                     /gene="isdB"
                     /locus_tag="O0286_002024"
     CDS             45..1982
                     /gene="isdB"
                     /locus_tag="O0286_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCY0170389.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(2136..2309)
                     /gene="rpmF"
                     /locus_tag="O0286_002025"
     CDS             complement(2136..2309)
                     /gene="rpmF"
                     /locus_tag="O0286_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCY0170390.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(2389..2946)
                     /locus_tag="O0286_002026"
     CDS             complement(2389..2946)
                     /locus_tag="O0286_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCY0170391.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            3073..4212
                     /locus_tag="O0286_002027"
     CDS             3073..4212
                     /locus_tag="O0286_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCY0170392.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(4274..4756)
                     /gene="coaD"
                     /locus_tag="O0286_002028"
     CDS             complement(4274..4756)
                     /gene="coaD"
                     /locus_tag="O0286_002028"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCY0170393.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(4758..5300)
                     /gene="rsmD"
                     /locus_tag="O0286_002029"
     CDS             complement(4758..5300)
                     /gene="rsmD"
                     /locus_tag="O0286_002029"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCY0170394.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            5370..5759
                     /locus_tag="O0286_002030"
     CDS             5370..5759
                     /locus_tag="O0286_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170395.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(5762..6016)
                     /locus_tag="O0286_002031"
     CDS             complement(5762..6016)
                     /locus_tag="O0286_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCY0170396.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            6255..7181
                     /locus_tag="O0286_002032"
     CDS             6255..7181
                     /locus_tag="O0286_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0170397.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(7244..7678)
                     /locus_tag="O0286_002033"
     CDS             complement(7244..7678)
                     /locus_tag="O0286_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCY0170398.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(7694..8731)
                     /locus_tag="O0286_002034"
     CDS             complement(7694..8731)
                     /locus_tag="O0286_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0170399.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(9058..9519)
                     /locus_tag="O0286_002035"
     CDS             complement(9058..9519)
                     /locus_tag="O0286_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCY0170400.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(9544..10455)
                     /locus_tag="O0286_002036"
     CDS             complement(9544..10455)
                     /locus_tag="O0286_002036"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCY0170401.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
BASE COUNT         3826 a         1861 c         1488 g         3400 t
ORIGIN      
        1 tgattatcag ttgataatat acaattagga gttgtttcta caacatgaac aaacagcaaa
       61 aagaatttaa atcattttat tcaattagaa agtcatcact aggcgttgca tctgtagcaa
      121 ttagtacact tttattatta atgtcaaatg gcgaagcaca agcagcagct gaagaaacag
      181 gtggtacaaa tacagaagca caaccaaaaa ctgaagcagt tgcaagtcca acaacaacat
      241 ctgaaaaagc tccagaaact aaaccagtag ctaatgctgt ctcagtatct aataaagaag
      301 ttgaggcccc tacttctgaa acaaaagaag ctaaagaagt taaagaagtt aaagccccta
      361 aggaaacaaa agaagttaaa ccagcagcaa aagccactaa caatacatat cctattttga
      421 atcaggaact tagagaagcg attaaaaacc ctgcaataaa agacaaagat catagcgcac
      481 caaactctcg tccaattgat tttgaaatga aaaagaaaga tggaactcaa cagttttatc
      541 attatgcaag ttctgttaaa cctgctagag ttattttcac tgattcaaaa ccagaaattg
      601 aattaggatt acaatcaggt caattttgga gaaaatttga agtttatgaa ggtgacaaaa
      661 agttgccaat taaattagta tcatacgata ctgttaaaga ttatgcttac attcgcttct
      721 ctgtatcaaa cggaacaaaa gctgttaaaa ttgttagttc aacacacttc aataacaaag
      781 aagaaaaata cgattacaca ttaatggaat tcgcacaacc aatttataac agtgcagata
      841 aattcaaaac tgaagaagat tataaagctg aaaaattatt agcgccatat aaaaaagcga
      901 aaacactaga aagacaagtt tatgaattaa ataaaattca agataaactt cctgaaaaat
      961 taaaggctga gtacaagaag aaattagagg atacaaagaa agctttagat gagcaagtga
     1021 aatcagctat tactgaattc caaaatgtac aaccaacaaa tgaaaaaatg actgatttac
     1081 aagatacaaa atatgttgtt tatgaaagtg ttgagaataa cgaatctatg atggatactt
     1141 ttgttaaaca ccctattaaa acaggtatgc ttaacggcaa aaaatatatg gtcatggaaa
     1201 ctactaatga cgattactgg aaagatttca tggttgaagg tcaacgtgtt agaactataa
     1261 gcaaagatgc taaaaataat actagaacaa ttattttccc atatgttgaa ggtaaaactc
     1321 tatatgatgc tatcgttaaa gttcacgtaa aaacgattga ttatgatgga caataccatg
     1381 tcagaatcgt tgataaagaa gcatttacaa aagccaatac cgataaatct aacaaaaaag
     1441 aacaacaaga taactcagct aagaaggaag ctactccagc tacgcctagc aaaccaacac
     1501 catcacctgt tgaaaaagaa tcacaaaaac aagacagcca aaaagatgac aataaacaat
     1561 taccaagtgt tgaaaaagaa aatgacgcat ctagtgagtc aggtaaagac aaaacgcctg
     1621 ctacaaaacc aactaaaggt gaagtagaat caagtagtac aactccaact aaggtagtat
     1681 ctacgactca aaatgttgca aaaccaacaa ctgcttcatc aaaaacaaca aaagatgttg
     1741 ttcaaacttc agcaggttct agcgaagcaa aagatagtgc tccattacaa aaagcaaaca
     1801 ttaaaaacac aaatgatgga cacactcaaa gccaaaacaa taaaaataca caagaaaata
     1861 aagcaaaatc attaccacaa actggtgaag aatcaaataa agatatgaca ttaccattaa
     1921 tggcattatt agctttaagt agcatcgttg cattcgtatt acctagaaaa cgtaaaaact
     1981 aataaatcgt ctttatattt aattattaaa ttaacaaatt ttaattggcg gatgaggtat
     2041 ccagttacct cgttcgccaa ttatttttcg caatataaaa agtcccactt aaaacaatca
     2101 ttttaagcgg gactttttat attgagtaac taaaattatt tagctgctac ttcttcgcca
     2161 ttgtaagaac cacagttttt acatacacgg tgtgataatt tgtattcgcc acagtttggg
     2221 cattcagtca tacctggtac tgaaattttg aaatgcgtac gacgtttgtt ttttctagtt
     2281 ttagaagttc ttctttttgg tactgccatg atatatcctc cttagattat aaacgaaaaa
     2341 tactaaatgt tagtttaatt aacaacatta tatcattaat taaactactt attgctcttt
     2401 atcatataat tgttgtaatt tttgaagcct tggatcaact tgtcgtgatt ctgaatcatc
     2461 ttgttcttgc tgtttagcaa gctcatctaa ttgatcctca tcgattactt cccaaccatt
     2521 acctactgtc aacatttggt cactttgctc tgaataagct ctcattggtt tctcaataat
     2581 aactatatcc tcgacaatat cctgaagatt aaccatacca tctttaataa tgtgatagtg
     2641 ttcatctaca tcatcttgat catcgttata ctgattgtac ccttctaaat caaatacttc
     2701 tgtagtagtt acatctagtg ggacttttac tggtacaaga gtacgtgcac aaggcattgt
     2761 atacgttcca gtaatgtgaa tatccgcaac gacttctgtt gacttaatgg ttaactgacc
     2821 ttggattgta attggagata aatcaattaa atctaatgat tcttttaaat tgtcaaaact
     2881 caccgtttga tcaaattcaa atggcttacc ttgatatttc cttaattgcg taattgacca
     2941 tttcatatgg cttcacctct aacaagcaca aaatttattt taactttcta agaacaagtt
     3001 gtcaagattt tttcttaaca tatcatgttc tgttacaata ttaataatga ataccgaaag
     3061 gacgaattgc aaatgaaaag cgttggctta atcacagaat ataatccctt tcataatggg
     3121 catcaatatc atattaatca atctaaaaaa cttacaaatg ctgacgttac tattgcaata
     3181 atgagtggta actttgtcat gcgtggcgaa ccagcaatct ataataagtt tactcgtgca
     3241 aaaatggcat tatcaacagc tgatttagtt atcgaactac cagcaactgc cagtttatca
     3301 tctggcgatc attttgccga actagcagtt aaagtcgcag attatatgag tgtcgataca
     3361 attgcatttg gtagtgaaaa taatgatatc aaaacattaa agcaattagc acacagcatt
     3421 aatgaaattg aacaatctga atccttttca caaaaagtaa aagaaggtaa aagctaccct
     3481 cgtatcatta gcgaattatt agagcatcat gaagcactag caagtcctaa caacatactt
     3541 ggtattagtt acctgaaagc aattgctaaa aatgctaaaa acatcaatgc aatttctatc
     3601 aaacgagaaa atgctcaaca tcatgattca ttaattcaac accatcagtt tgcaagtggt
     3661 acatctatta gaacatcaat cattagtcaa gatgatcatt ggcatcatgt ggtacctaaa
     3721 gatattcaac acttgtacgt aacacctcat ataacattaa atcaaatatt tccatatttg
     3781 aaatatcaaa tcatagctat gactacagat tcattaaaaa atatttatac tgttactgaa
     3841 ggttttgaaa atcgtttgaa atctaacatt tatgaagcaa ctgattttca tcattttgta
     3901 aaattactaa aaacaaagcg ttatacatat acgcatatcc aaagactgtt aatgaatgta
     3961 ttattaaaca ttaaacctac agatgttacg agtaacattc acgcagttaa agtattagcg
     4021 atgaacgacc gtggcagaca atatttaaag catttaaaaa cagcgttccc agaaagacaa
     4081 tatataacca atattaacaa aagcaatgca cattacttca caaatgaaat caaagcaaca
     4141 cacatataca atgcaataag cgggcaacaa caaacagatt tcaacacgcc tgtcatacag
     4201 caatatcgtt aacaaatgaa aacagtattt taggattgta aaccatgata aacctaaaat
     4261 actgttattt ttattactta aatttcttct tcaatgcctt ttcaacataa ggtggaacga
     4321 attcagaaat atctgctcga taagctgcaa cttctttaac aatacttgaa cttataaatg
     4381 aataattagt actagacatc atatataacg tttcaatttc attgttcaac tttttattca
     4441 ttgaagttaa gcgtaattca tattcaaaat cactgactgc tcttaaacca cgtatgattg
     4501 ttttagctcc tacttgttca caataatcga ctagtaaacc actaaattga tgaaccttga
     4561 cattaggtaa atgtttaaca gattgttcaa ttaaatccat acgctcttct aaactaaacg
     4621 taccttcttt tttactattt ttaagaacac agacatgaat ttcatcaaat ctatctgtac
     4681 ttctctcaat aatgtctaaa tgaccataag taatggggtc aaaactaccc ggaatgaccg
     4741 ctattgtatg ttccatgcta ttctcccttt tctaataaca atgtgtctgt caacccataa
     4801 tggtaacgtt taatcatatt aaacggttga taatctattt cttcatgatt gctaaattca
     4861 caaacgatga taccattttc tttcaataaa ttaaactctg aaattagttt taaagcttta
     4921 tcaatgagac ctttattata aggtggatct aagaaaatga catcaaattg aatatcacgt
     4981 tttgacaatg cttttaaagc tctatctgca ttatttttat aaacttcaga ttgtgcctct
     5041 aaatccaaat tcgcaagatt tgatttaata acttttacag ctttaaaatt ttgatcaaca
     5101 aagattacct tatccatacc tcgagagagt gcttctattc caagcgcccc gcttcctgca
     5161 aataaatcta aacctatacc tgacacatca tataaactat taaagatacc ttctttaact
     5221 ttatccatag ttggtctcgt attacggcct tccatacttt ctaaagcttt acttttatgt
     5281 ttacctgcaa tgacgcgcat gttgttcaca cttccaattc atttagttat ttaatataat
     5341 ttattgagaa aaaggagaat gataaaccaa tgaaacaaac atttattaca cttggtgaag
     5401 gtctaacaga tttgttcgaa ttcatgacga tgattgaata taaccatcaa cgtattgata
     5461 aaattatcta ttttcattca ccacaagctg aaaataaaaa gtcatctgta gcaatcatta
     5521 tgaaccctac aactggcaat catttccaag cattttatat catgataaac gctattaaat
     5581 atccatatcc agattcaaat aaaaagtttc aaatgataaa tgattgtgct gaaaaattcg
     5641 acataccaat tttaggtatc gatgtacagc cccctcaagc atttcatgat ttatcgttat
     5701 attataatta tttaattagt gtgttaaggc tccaaaaatg gataccagaa cttcaataat
     5761 attaattata tatttcgtgt ttctcttttt cgtaagtttt ctttaagtat ttatatggtg
     5821 agccatcaat gtgtctaacg tatttaagtt gcattagttt atgtacaatt tgatcaacat
     5881 cttgctcatt cacatacata attacaaatt tacgatctct atttgaatga acgatatgtc
     5941 catattttcg gatttgtcgt tcatgtttca tatgttttaa ataaactaca atactagttc
     6001 ttgggattaa attcatttaa acctcactcc atttattttc agcattaatc atatcatgtc
     6061 tttattaatt ttaaaatatt aagctacctt aattatgtct tatgtaattg ctcgataaat
     6121 gtatttagat tatattaaac atcattatat taattcaatt ctcaagagca tcactatatg
     6181 attaaccaca catttcgctg tagtcagaac tatgaatttg ttaagctata aaagtaaacg
     6241 aggaggaatt ttttatgaaa aaagtaaaca gaaacatttt aaatgcggta ctagtcggtg
     6301 ctggattttt aagtagtcta ttaatgatca ataaaaataa agtgattact aaaaagcaaa
     6361 ccatccctgc ttttttcaaa ggcaatgcac catatatatt tgcgcatcgc ggtggtatgg
     6421 ctttaagacc agagcaaacg caacttgcat ttgactatgc taaacaatta ggcgttgatg
     6481 gctttgaaac agatgtgaga ctcacaaaag atcaacagct tatcgttttt catgacgcaa
     6541 ctgttgatag aacgacgaac ggctctggaa aagttagtgc ccatacatta gcagaattaa
     6601 agaaattaga cgcagcttat cacttcaaag atatcaatgg actaacacca tatcgtggtc
     6661 atgcacacac agctatacta accttcgatg agttgctgaa gcaatatcct gatatgtata
     6721 taaacgtaga cttaaaagat gctcctgaat cctatgaagg ctcaatcgca ccacaaatta
     6781 tgtttgatac aattgcagaa aatcaagcat ttgaccgcgt tcttgtgaca agtttttata
     6841 aagaacaaat cgttcgtttt aataaaattg cacaaggatc cgtcgcaatt ggtgctagcc
     6901 aacaggaagt tacagaagca tttttaaaat atcatttatt aggtggtagg tactaccaac
     6961 cattagcaca aacgttccaa atgcctactc attttaaagg cattgatttg acttcatctc
     7021 gttttatcaa atggttaaat gatatgaata tcattccagg ctattatggc gtaaatagta
     7081 taaatctgat gaatgattta taccaaaaag gtgcacacac gattgtgaca gatcgaccag
     7141 atttagcaca acaattcaaa caaacaatcc cgaataaata aaaacatcga gtcagatgct
     7201 agttagtggt ttcaataacc acctttttct gactcgatgt tttttataat gaacaattac
     7261 aggatccgcc cgtcgcacaa ccactatgtg atgtttgaaa gaatggatta cctgtttcta
     7321 ttttgacatg ttctgaaaca gcgtacgcaa ttttcgtaac aacttcatct attaaatgtt
     7381 gaagtgccat ttccctagct ttgtaatgca tcacaacgtc aagcatctca tatgctcttt
     7441 tgcgttgtct tgtctctaac ataactttct tataatcagg atgatatttc ccgaatcgca
     7501 ttacttcatc atatttctct tttgatttta aaaatgattg ataaagtaaa tgtgcttcgt
     7561 cattgttttc gagcgcctgt ttagcttgct caaatgatgc ataaatatct gatgcaacta
     7621 tcatatcagc tagttcatca atatcatcta atatcgctag tgaagcttca ttaatcataa
     7681 atacccctca ttgttatttt tctaagaaaa ttagtgaata ataatctctc attacatctc
     7741 cacccatctc atcgtatttt gaatttaata gacgagatcg atgtatatct gaattcatcc
     7801 aactatggat taatgttggt acatcattaa acgcataacc aacattttga gcagttgttt
     7861 tataagtaac gtgattttta tctaattgcc ctcttaatgc gtcctctgta aattcaacac
     7921 tatcagaacc attagaggtc gcttcatata agttattaga tgcaatatgt gctaaatcgc
     7981 tattgatttt caatggtttt aatcctttta attttctcat ttcattcgtt acttcataaa
     8041 gagaaattaa ttgatttgga ttttgctcaa ctggacgctt attatgctct tctgacgtag
     8101 aattagaatt taattgataa ggttcaatat ctgctaacat ttcttttgtt aaaaatcgta
     8161 cacttagcac ctttttcgat tgttgatcag aatacacttg tgcatatatg tcgccatatt
     8221 taatcagtgt ttgtgttttt aaatcttcat ctgaaagttc aaattcatat tttttaccat
     8281 caactttaaa ggacggttct ggattaatac ttgtatgatt aaaaatttct gcagaatgtt
     8341 gtcctatttt taacggacta acattgactt tctcacctgt agcatacact gaaacgattt
     8401 cttcacgttt agttgaaaca atgtaataac tgtttttgtc tttaaacaca taatttttgt
     8461 aaccatctct aaaagggtag acacgatctg cttgtccaaa tttagaagta agcactttaa
     8521 tatctttacc tacccatgta ccaacacctt ctttaggctt tggattttca gcatggttat
     8581 ttgatttatt cacctgttca gatctattta ctttattacc tttattcggg ttctctaata
     8641 catcaaattt taatctcggt gaataaaaaa gatatattaa aaatccaact aaaaataata
     8701 caccgacgac tcttataatt aactttttca tcaatcaacc acctaaaaag tattaatact
     8761 attgtaaaaa acaacacatt aattagcaaa ttttcaacac tgacataact gtgtcgtttc
     8821 gataaaacaa aatcatcttc aggcataatt ttaacgtcat aaagattttc cctacactct
     8881 atatcataac ctatctttat gttttcaggt tgaatttcat tattaagatt aaaatatgta
     8941 taaaaaaatg gacagttaag gtatcaaatg aataccatca actgtccgac tattcttcat
     9001 caaaaaacct gataaaacaa attgccttat cagattagta tcatttgtat aagcatatta
     9061 atgaccaaat gttgctttaa tcagtgatgt cgtttctcca cctggatata atacatataa
     9121 taataagtaa actgctacac ctgtaattgc agtacagaac catataactg aagcgaatgg
     9181 cccgaatttg cggtgtacat tatatttatc tttaaatgca gtaataattt gaactaggcc
     9241 tagaatacca ccaattgttg ctaaattaat gtggaaaaat aagaaaatcg tataatattt
     9301 cttaattgat gctggtccgc caaatgctgt attaccgata aaaatcgttc tacttgcata
     9361 gattaagaaa aaagttaaag caaaaacagc tgcagctaac ataacatttt tgtgtttatt
     9421 tatttccctt ttccaaataa gtctccaacc aatggcaatt aaaattgcac taatgacaat
     9481 acatgtcgta ctaatcgttg gtaaaattgg aacgcccata tttttcatcc taacttaatt
     9541 aatctagatc aaagtaagta atgaaacaat cacagctaac acgaaaaaga tcactaaata
     9601 atttagtgaa tatataaaca tttgtgttgc ccattttgtt tgatctgaat ttttcttaaa
     9661 tgttgttaaa cctaatgcaa tccatcctaa atttaataat gtagctaaca ctacgaatac
     9721 gacacctaaa tttattagta ataaaggtac tggcaataaa ataatcaacc agataaacat
     9781 actgacacgt gtacgtttaa agcccttaac tgatggtaac attggaatat ttgcaagtgc
     9841 atattcatct ttacgtttaa tagctaaggc ataaaaatga attggttgcc aacaaaatac
     9901 aactaaaaac agcgcaatcg ctgttaaact aatttgtcct tcaattgcaa cccatccaat
     9961 tagtggtggt actgctccag gaaaactccc aatcactgtg ttccatgttg tatgtctttt
    10021 agaccatatt gagtaataag acacataacc tacaatcccc ataagaccaa gtacgcctga
    10081 tggtatattc aataaaaaca aacaaatttc tccaactaac atcataccaa aacttaatag
    10141 taataaattt tgatctgtaa ttctattatt tacagttggt ctattttgtt tactaggcat
    10201 aatacgatca atatcttggt cgtaataatt atttaacgca catgcgccac ccataattaa
    10261 agtagatcca aatagcatta ataaaatttg aggtattgat gataagaagg aatgatttgt
    10321 cattacaact gctagccatg cgcccgcaaa agccggaatt aagttacctt gaacaagtcc
    10381 cattttaatt atctgttgca attctttgaa gttaactctg ctaatatttt gtgacaaagt
    10441 atgctctttg ctcataatcc ccctccttaa atttgttcat ataagattat gatatcttag
    10501 attgcataaa aagactaggt ttaataaaat taaattgtga caaattaacg acaagagaaa
    10561 atgtcaattt tgtga
//
LOCUS       DAKTGS010000101        10512 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.101, whole genome shotgun sequence.
ACCESSION   DAKTGS010000101 DAKTGS010000000
VERSION     DAKTGS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.101"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            82..315
                     /locus_tag="O0286_002037"
     CDS             82..315
                     /locus_tag="O0286_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170402.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            451..690
                     /locus_tag="O0286_002038"
     CDS             451..690
                     /locus_tag="O0286_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0170403.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            811..2799
                     /gene="tkt"
                     /locus_tag="O0286_002039"
     CDS             811..2799
                     /gene="tkt"
                     /locus_tag="O0286_002039"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCY0170404.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            3077..3319
                     /locus_tag="O0286_002040"
     CDS             3077..3319
                     /locus_tag="O0286_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCY0170405.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            3306..3404
                     /locus_tag="O0286_002041"
     CDS             3306..3404
                     /locus_tag="O0286_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170406.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            3498..3965
                     /locus_tag="O0286_002042"
     CDS             3498..3965
                     /locus_tag="O0286_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCY0170407.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            4089..5210
                     /locus_tag="O0286_002043"
     CDS             4089..5210
                     /locus_tag="O0286_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0170408.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            5214..8243
                     /locus_tag="O0286_002044"
     CDS             5214..8243
                     /locus_tag="O0286_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCY0170409.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQSDYEKIIELNNTITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEI
                     KSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIK
                     FVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRDLNKFIEQMKEEKDNLTLQIH
                     NKQLRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVNQHQELSKEIED
                     KLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTDDNDIDEIIAW
                     RGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGKMNTLI
                     DEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFV
                     LIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLS
                     GGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLLNLKST
                     GRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(8314..8676)
                     /gene="mscL"
                     /locus_tag="O0286_002045"
     CDS             complement(8314..8676)
                     /gene="mscL"
                     /locus_tag="O0286_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCY0170410.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            8880..>10512
                     /locus_tag="O0286_002046"
     CDS             8880..>10512
                     /locus_tag="O0286_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0170411.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIE"
BASE COUNT         3906 a         1398 c         1884 g         3324 t
ORIGIN      
        1 catttgttcg ttaaaatatt gacacagaac ataagttctg ataaattact aatacaaaca
       61 aatgttctag gagtgaaaat gatgttttac aataaatata aaaacgtatc aacatatatc
      121 atcatatttt tagtttcaag tgcagccttt gcaatattct tgttaagtgc gaacattagt
      181 gctcactcgg aacaagtgta cgaaatgact gaccatcaaa ttaagaacaa tacgataaat
      241 aaagcatacg aacataaaga ccctacaaac aatagcgaac aaagagatgg gaaagtgttc
      301 gctttaataa attgatacat tgtcacaacg ttattttgcc tatttttgcg aaatagcgtt
      361 ttttattact tttttgctga tcttaaattt gttatatttt gttaaagtat tataatgatt
      421 gaataaacaa attgaaggta ggttttttaa ttgagtaatt ctgatttgaa tatcgaaaga
      481 attaacgagt tagctaaaaa gaaaaaagaa gtaggattaa ctcaagaaga agcaaaggag
      541 caaacagcct taagaaaagc ttatcttgag agttttagaa aagggtttaa acaacaaatt
      601 gaaaatacta aagtaattga tccagaaggt aatgatgtaa cacctgaaaa aattaaagag
      661 atacaacaaa aaagagataa taaaaattaa atcacaaatc tgtaaagaat tttctgacat
      721 tataacttga aataagtatt ttacttatct ttttatttta aaataagtta taatgtattt
      781 gataaaattg aagaagggaa gatacacaag atgtttaatg aaaaagatca attagctgtt
      841 gatacgctac gtgcactaag tatcgacaca atcgaaaaag cgaattctgg tcatccagga
      901 ttacctatgg gagctgcccc aatggcttac actttgtgga cacgtcatct gaattttaat
      961 ccacaatcta aagattactt caatagagac cgtttcgtat tatctgcagg gcatggttca
     1021 gcattattgt atagcttgtt acatgtttct ggtagtttag aattagaaga attaaagcaa
     1081 tttagacaat ggggttctaa aacaccaggt catcctgaat acagacatac agatggtgta
     1141 gaagttacta ccggaccact tggacaaggt tttgctatgt cagtaggatt agctttagca
     1201 gaagatcacc tagcagggaa atttaataaa gaaggatata atgttgtaga tcattacaca
     1261 tatgtattag cttctgacgg tgatttaatg gaaggtatat cgcatgaagc agcttcattt
     1321 gctggacata ataaattaag taaattagtt gttttatacg attcaaatga tatttcatta
     1381 gatggcgaat taaacaaagc tttttctgaa aacacaaaag ctcgttttga agcatatggt
     1441 tggaattact tactagttaa agatggtaat gatttagaag aaattgataa agcgattact
     1501 acagctaaat ctcaagaagg accaacgatt attgaagtta aaacaacaat cggatttggt
     1561 tcaccgaata aagcaggaac taatggtgtt catggggcac ctttaggtga agttgaaaga
     1621 aaattaacat tcgaaaatta cggtttagat cctgaaaaac gttttaatgt ttcagaagag
     1681 gtatacgaaa ttttccaaaa tactatgtta aaacgtgcta atgaagatga atctcaatgg
     1741 aattcattat tagaaaaata tgcagaaaca tatcctgaat tagcagaaga atttaaatta
     1801 gcgattagtg gtaaattgcc taaaaattat aaggatgaat taccacgttt tgaactgggt
     1861 cataatggtg catctcgtgc tgattctggt actgttattc aagcaatcag taaaactgtc
     1921 ccttcattct ttggtggatc agcagacctt gctggttcaa acaaatccaa tgtaaatgat
     1981 gcaactgatt atagttctga aacacctgaa ggtaaaaatg tgtggtttgg tgtacgtgaa
     2041 tttgctatgg gtgctgctgt aaatggtatg gctgcacatg gaggtttaca tccatatggt
     2101 gcaacattct tcgtatttag tgattattta aaaccagcgt tacgtttatc atcaattatg
     2161 ggattaaatg caacgttcat cttcacacat gattcaattg cagtaggtga agatggtcct
     2221 actcatgaac caattgagca attagctgga ttaagagcca ttccaaatat gaatgttatc
     2281 cgtcctgctg atggtaatga aacaagagta gcatgggaag ttgccttaga atctgaatct
     2341 acacctactt cattagtatt gacacgtcaa aacttaccgg tattagatgt accagaagat
     2401 gtagttgaag aaggcgttcg aaaaggtgcc tatacagttt atggctctga agagacacca
     2461 gaattcctat tattagcttc aggttcagaa gttagtcttg cagttgaagc tgctaaagat
     2521 cttgaaaaac aaggtaaatc agtacgtgtt gtttcaatgc ctaactggaa tgcatttgaa
     2581 caacaatctg aagaatataa agaatcagtt attccatcaa gcgtaacaaa acgtgttgcg
     2641 attgaaatgg cttcaccgct tggatggcat aaatatgtag gtactgcagg taaagttatt
     2701 gctattgacg gctttggcgc aagtgcacct ggcgatttag tagttgaaaa atatggattt
     2761 acaaaagaaa atatcttaaa ccaagttatg agcttataag aataatttat aaagcgagta
     2821 tgtttagaag tctaggatgc ataatcttag gcttctttta aagtgttgaa atttagagta
     2881 tagcacttaa actacatcat aagtgataag ttatgaaagt atactatttc agattaatct
     2941 ttaaaagctc tgttataaca gcatgatttt tgatattatt tttagtatcg atattaaaat
     3001 acttgaataa actagttctt gaaataatgt gatgaattta gtaaaattca gtaggataaa
     3061 gaaagcgggt gaaacaatgg caacttggtt agcaattatt tttatagtag ctgcattaat
     3121 tttaggttta attggaggtt tccttttagc tagaaaatat atgatggact acttgaagaa
     3181 aaacccacca atcaacgaag aaatgcttcg tatgatgatg atgcaaatgg gtcaaaaacc
     3241 ttctcagaag aaaattaatc aaatgatgac gatgatgaat aaaaatatgg atcaaaatat
     3301 gaagagtgcg aaaaagtaaa ttcgcaattg atagaggcta ttttccagat atggaaatgg
     3361 cctcttttta taatcaaatt aataagaata aatatgttta ttaaaattaa agttaacaaa
     3421 atgacgaata gactgagaaa tgctataatt cattttgtat gatttacaga gagtttattt
     3481 aacgagaagg tgtctgcgtg ctctatttaa tattttcaat cattgtagct ttatttatgg
     3541 gaactatagt tatagttatt cgtatgaaag ctcaaaatta tccggtaaat gagaaaaaaa
     3601 tagttttgcc accgtttttt atggcgaccg gtgcattgat gtacgtcgtt ccatatttta
     3661 ggctaacagg atcggaaatg ctagaagcct ttataattgg tttgcttttt tctacagttc
     3721 taatttggac ttctcgattt gaagtcaaag gtacagaaat ttatatgaaa cgatctaaag
     3781 catttccagt tattttgatt tcattactta tcattcgtac tgtgatgaaa atattcatta
     3841 gtaatgaaat agatcctgga gaattaggcg gcatgttctt tttattagca ttctgtatga
     3901 ttgttccttg gagagcagca atgctatata aatacaaaaa actaaagaaa acattaatca
     3961 attaattact tttaaaacca cttgtgatcg acttctaaat cagtcaatga gtggttttaa
     4021 ttttacttga aaaggtgaaa tgattatatt aaaataaaag cgaacaaatg ttctatatgg
     4081 aggcagtaat gaaaattata catacagcag actggcactt agggaaaata ttaaatggca
     4141 aacagctttt agaagatcaa gcgtatattt tagatatgtt cgtagaaaaa atgaaagaag
     4201 aagaacctga tatcattgtg atagctggag atttatatga cacaacatat ccaagtaaag
     4261 atgcaatcat gttattagaa caagcgattg gaaagttaaa tttagaactg cgtataccaa
     4321 taattattat tagtggaaat cacgatggta aagagaggtt aaactatggg gcgagttggt
     4381 ttgaacataa tcagttattt ataagaacag attttacatc gattaattca ccaatagaga
     4441 taaatggggt taatttttat acactccctt atgctactgt gagcgaaatg aaacactact
     4501 ttgaagatga caccattgaa acacatcaac agggaattac gcgctgtatt gaaacaatag
     4561 caccggaaat tgatgaagat gccgtcaata ttttaattag tcatctgact gttcaaggtg
     4621 gaaagacatc tgattctgaa agaccattaa ctattggaac ggttgaatca gttcagaaag
     4681 gtgtttttga tatatttgat tatgtcatgc taggtcactt gcatcatcca tttagtatag
     4741 aagacgacaa aattaaatat agtggctcct tattgcagta ttcattttcg gaagcgggtc
     4801 aagctaaagg gtatagacgt gtaacaatta atgatggcat tattaacgat gtatttattc
     4861 ctcttaagcc acttagacaa ttggaaatta tctcaggcga atataatgat gttattaatg
     4921 aaaaagttca tgtgaaaaat aaagataatt atttacattt taaacttaaa aatatgtctc
     4981 atattactga tccaatgatg agtttaaaac aaatttatcc taatacttta gcgctgacga
     5041 atgaaacttt taattacaat gaagaaaata atgctataga aataagtgaa aaagatgaca
     5101 tgtcaattat cgaaatgttt tataaacata taactgataa agaattatcg gatatccaat
     5161 ctaaaaagat aaaaaatatt ttagaaaacg aattgagaaa ggaggattaa tgaatgaaac
     5221 cattacattt aaagttgaat aatttcggcc cctttttaaa agaagaaatt gatttttcta
     5281 aaattgataa taatgaattg tttttaataa gtggtaagac tggatcgggt aaaacaatga
     5341 tttttgatgc aatgacttat gccttgtttg gtaaagcatc aactgaacaa agagaagaaa
     5401 atgatttgag aagtcatttc gctgatggta aacagccgat gtcagtaaca tttgaatttc
     5461 aattaaatca tcgaatttat aaagtgcata gacaaggccc ttatatcaaa gaaggtaata
     5521 caacaaaaac gaacgctaaa tttgatgtat ttgagatggt ggatggcaag tatgaaatta
     5581 gagaaagtaa agtaatttca ggtacccaat tcattattga attattagga gtaaatgcag
     5641 atcaattccg acaattgttt attttgcctc aaggtgaatt caaacgcttt ttaatatcaa
     5701 acagtcgtga aaagcaaggg atattaagaa cactgtttga cagtgaaaaa tttgaagcta
     5761 tacgagaaat attaaaagaa gaagtaaaaa aagaaaaagc tcaaatcgag aatagatatc
     5821 aacaaattga ccttttatgg caagaaattg aatcatttga tgatgacaat ataaaaggct
     5881 tattagaggt tgccactcaa cagatagaca aattgattga aaatatacca cttttacaag
     5941 ctaggtcgaa agaaatacta gcatctgtaa atgaaagtaa agaaactgct attaaagaat
     6001 ttgaaataat agaaaagaaa acattagaaa ataatatatt aaaagataat attaatcaac
     6061 tcaacaaaaa taaaattgat ttcgttcaat tgaaagaaca acaacctgaa atagagggaa
     6121 ttgaagctaa gttaaagttg ttacaagata ttacaaacct attgaattat attgaaaata
     6181 gagaaaaaat tgaaactaaa attgctaata gcaaaaaaga tatttctaaa accaataata
     6241 aaatattgaa tcttgattgt gataagcgaa acatagacaa agagaaaaaa atgttagaag
     6301 aaaatggaga tttaattgaa agtaaaatct cttttattga taaaactaga gtattattta
     6361 acgatattaa taagtatcaa caaagttatc tcaatattga acgcttgaga actgagggtg
     6421 aacaattagg tgatgaatta aatgatctaa ttaaaggttt agaaacggtc gaagattcaa
     6481 taggtaataa ccaaagtgat tacgagaaaa ttatcgaact aaataatacg ataacgaaca
     6541 taaataatga aattaatata attaaagaaa atgaaaaagc taaagctgaa ttagataaac
     6601 tattaggtag taagcaagag ttagagaatc aaattaatga agaaacatct atattgaaga
     6661 atctcgaaat aaaattagat cgctacgata aaacaaaatt ggacttaaat gataaagaaa
     6721 gctttataag tgaaattaaa tctgctgtaa atattggaga tcaatgtccg atatgtggta
     6781 atgaaattca ggatttaggg catcatattg attttgacag tattgctaaa cgtcaaaatg
     6841 aaattaaaga aattgaagca aatatccacg ctataaaatc gaatattgct gtgcataatt
     6901 ctgaaattaa atttgttaat gaaaaaatat cgaatattaa tattaaaacg caaagtgatt
     6961 tttcacttga agtattgaat aagcgtctgc tagaaaatga aaatgcattg aataatcaaa
     7021 gagatcttaa taaatttata gaacaaatga aagaagaaaa agataatcta acgttgcaaa
     7081 ttcataataa acaattgcgt ctaaataaaa atgaatctga gttgaaatta tgtcgagatc
     7141 tcatcactga atttgaaaca ctctcgaaat ataataatat cactaatttt gaggtggatt
     7201 ataagaagta tgttcaagat gtgaatcagc atcaagaact ctcaaaggag attgaagata
     7261 agttaatgca attgtctcaa agaaagttaa ttgagcaaaa taatctaaat cattatgaaa
     7321 atcaactaga aacttacaat aatgacttag aattgaatga acagtctatt gaaatggaaa
     7381 tgtcgaggct gaatttaact gatgacaatg atatagatga aataatagcc tggaggggcg
     7441 agcaagagga attagagcag aaaagggata cttataaaaa acgttatcat gaatttgaaa
     7501 tggaaatagc taggttagaa tcattaacta aggataaaga gttattggac tctgataaat
     7561 taaaagatga atatgagcta aaaaaaggaa agatgaatac actgatagat gaatactctg
     7621 ctgttcatta tcaatgtcaa aataatatta ataaaacaca atctatagtt tcgcatatta
     7681 attacttaaa tcaagaatta aaggatcaac aagaaatatt tcaattggct gaaattgtca
     7741 gtggtaagaa taacaaaaat cttacattgg aaaactttgt cttaatttac tatttagatc
     7801 aaattattgc tcaagcaaat ctgagattag caacaatgtc agataatcga taccaactaa
     7861 ttaggcgaga agcggtttct catggtctta gtggcctaga aattgatgta tttgatttgc
     7921 attcaaataa gtctagacat attagctcgt tatcaggtgg agaaactttc caatcgtcgc
     7981 ttgcattagc tttagggtta agcgaaattg tacagcagca atcaggaggt atttcactag
     8041 aatcaatatt tattgatgaa ggattcggta cattagatca agaaacgctt gaaacagcgt
     8101 tagacacttt attaaatctt aaatcaactg gtagaatggt tgggattatt tcacatgtga
     8161 gcgaattgaa aaatagaata cctttagttt tagaagtgaa atcagatcaa tatcagagtt
     8221 caacaagatt caaaagaaat taaaacataa aaaatagaga tggtactttg gaatgtatag
     8281 aacaacctta gcccatctct ttttatttaa aaattatttt ttctcacgta ataaatctct
     8341 gatttcagtt aataacacaa cattttcttc cacaactgct tcttcttcgg cttcttcttt
     8401 cttcattaat gtatttgcaa tcttaacaaa gatgaataaa gcaaacgcga tgataataaa
     8461 gtcgataaca gattggataa ataaaccgta tttaataccc cagaatgacc attctttagc
     8521 aaaatcaact gatccgaaaa ttttaccaat taatggcata atgatatttt ctactaatga
     8581 agatataatc ttgttgaaag ctgcacccat cacaacagca attgctaaat ctaagacgtt
     8641 accttttaag gcgaactctt tgaattcttt taacattaca ctcaacctct cttttttaaa
     8701 tgtaacctca attatacaac aaataaaaaa attagaaaat agattattca agaaaatttg
     8761 attatcgcaa agacctttgt tatcctataa aggtgcgtga gttattgaat aaaatgttta
     8821 gataatattt ttattctact acataaaatt aaatatagaa ttgaagggag taattaatta
     8881 tgaattcttc ttcaccagag aatccaaatg gaaagaagta ttcaccagtc ttcatctata
     8941 gtgcaattgt tgttgctata gtcgtattac ttggtgcatt tttacctgaa caattcaact
     9001 atgttaccaa taatattaaa atgtggatta cagaaaagtt aggttggtat tatcttattc
     9061 ttactacgat tatcgtgttc ttctgtatat tccttatttt tagtcctatt ggaaaactta
     9121 aactaggtaa accaaatgac aaacctgagt ttaatacaat ttcatggttt gctatgttgt
     9181 ttagtgctgg tatggggata ggtttggtgt tttatggtgc agctgaaccg atggcgcact
     9241 ttgctacgcc acctacagca gatcccaaaa ctactgaagc ttatactgaa gctctacgtt
     9301 caacattttt ccattgggga ttccatgctt gggctgttta tggtgttgtt gcgttagcgt
     9361 tggcatattc gcaattccgt aaaggtgaac caggtttatt atctagaact ttacgtcctc
     9421 ttttaggtga taaagtagaa ggtcctattg ggatttttat tgacgtttta tctgtatttg
     9481 cgacaatcgt tggggtagcc gtttcgttag gtatgggtgc tctacaaatt aatggtggtt
     9541 tacattactt gttcaatgtt ccaaacaata cgtttgtaca agcgattatc atcattgttg
     9601 ttactatctt atttatagca agtgcatggt ctggattaag taaaggtatt caatacttaa
     9661 gtaacttgaa cattggttta ggtactattt taatggtagc tgctttaatt gttggaccaa
     9721 ctgttcttat tttaaatatg ttaactagct ctacgggtag tttactaaac acattcttgt
     9781 ttaatagttt tgatacagca gctttaaatc ctcaaaaacg tgaatggatg tcttcatgga
     9841 cactttatta ctggggttgg tggttaagtt ggagtccatt cgttggagtg tttattgcac
     9901 gagtttcaaa aggacgttca attagagagt tcatttctgg tgtcttgcta gttccagcaa
     9961 ttgttagttt tgtttggttt agtgtctttg gtgtattagg catcgagaca ggtaagaaac
    10021 acaaagaaat ttttgatatg actcctgaaa cacagctatt tggagtgttt aatcatgtgc
    10081 catttggcat tgttttatcg ttgattgcat tattattaat tgcatcattc tttattacat
    10141 ctgctgactc agcaacattt gtattaggaa tgcaaacaac atttggttca ttaaatccat
    10201 ctagtatggt aaaagttgtt tggggaattt cacaggcctt aatagcattt gtacttttat
    10261 tagctggtgg cggtaacggc gctgaagctt taaatgcgat tcaaagtgct gcaattataa
    10321 gtgcattccc attctccttt gtcgtcatac tcatgatggt aagtttctac aaggatgcga
    10381 accaggaacg taaattccta ggtttaacat tgactccgaa taaacatcgc ttacaagaat
    10441 atatcaagag tcaacaagaa gattatgaat ctgacattct tgaaaagcgt cagtcacgta
    10501 gaaatataga ga
//
LOCUS       DAKTGS010000102        10483 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.102, whole genome shotgun sequence.
ACCESSION   DAKTGS010000102 DAKTGS010000000
VERSION     DAKTGS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.102"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(25..762)
                     /gene="sdpB"
                     /locus_tag="O0286_002047"
     CDS             complement(25..762)
                     /gene="sdpB"
                     /locus_tag="O0286_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCY0170412.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            811..1140
                     /locus_tag="O0286_002048"
     CDS             811..1140
                     /locus_tag="O0286_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170413.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            1585..2457
                     /locus_tag="O0286_002049"
     CDS             1585..2457
                     /locus_tag="O0286_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0170414.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(2844..4223)
                     /locus_tag="O0286_002050"
     CDS             complement(2844..4223)
                     /locus_tag="O0286_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0170415.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            4503..4859
                     /locus_tag="O0286_002051"
     CDS             4503..4859
                     /locus_tag="O0286_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170416.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            4856..5041
                     /locus_tag="O0286_002052"
     CDS             4856..5041
                     /locus_tag="O0286_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170417.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(5137..5328)
                     /locus_tag="O0286_002053"
     CDS             complement(5137..5328)
                     /locus_tag="O0286_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170418.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(5339..5974)
                     /locus_tag="O0286_002054"
     CDS             complement(5339..5974)
                     /locus_tag="O0286_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0170419.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(6049..6966)
                     /locus_tag="O0286_002055"
     CDS             complement(6049..6966)
                     /locus_tag="O0286_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCY0170420.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            7234..7764
                     /locus_tag="O0286_002056"
     CDS             7234..7764
                     /locus_tag="O0286_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170421.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(7830..9434)
                     /locus_tag="O0286_002057"
     CDS             complement(7830..9434)
                     /locus_tag="O0286_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0170422.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            9764..>10483
                     /locus_tag="O0286_002058"
     CDS             9764..>10483
                     /locus_tag="O0286_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0170423.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLV"
BASE COUNT         3769 a         1888 c         1410 g         3416 t
ORIGIN      
        1 aacttaatgt cccgacctct ttatctacgc ataaatactt attactgata taacgaaaga
       61 aacaaaatta tttgctatat gtaatgcaat tgttgaacct aggtttcttc cagattttaa
      121 ataagtgaaa actaatatga tggatagtat gagatatgga ccaaactcaa acggcgactt
      181 tgcatcagtc acatgaataa atgcaaataa gaacaccgaa acaatactca tagctataaa
      241 attaaacttc ttacctaatt ctccaattaa aatatgtcta aatacgattt cttcaactat
      301 tggacctaca atcacaatta ataagaatgc tacaggtaaa aatgcaggca cttcaaacat
      361 tttatttagc tcaagttcat tggctgtttc actatattgc aaatgtttag gtagaaactg
      421 tgtcatatat tcatatgtat aaattaagat gagagcaata atatacgtta ttgacaatct
      481 aagccaatat tttttgatat acgcaaaacc agctcgaagc cttgatggca tcacttttaa
      541 atgaaataaa taaaatgcgc caatcccaat cgtatatgct aaagcttgtg tgatagtcgc
      601 tacaaatatc agattactat cgatttcata ataaccaaac aaaattggtc ctatgtaagc
      661 tgcaattgtg agtgcataaa atataacacc tataattgga attataagca aatctctcca
      721 tgctatatct ttaaacgtgt atttcttttt ttcattttcc actgttatat cctttcctgt
      781 ttaataattg atttttggag gtacttctac atgataaacg aaactaagta tatgagacaa
      841 caaattacta atttgattca aatcattgat acgattaaat ataatacttt aatgacagat
      901 tggaacattc aaacgcatat ttataaattt aaccaaatag ttactaatga attgaataag
      961 ttcaaaggct ttgaaacatc atatataata aacgaaaatc aagtttccta ttatgaaatt
     1021 ataacactac ttaataaacg tcccctccga caagtcgact atggtaacaa aattcaatat
     1081 cttaattttt atcatacaga actatctaac gcattatttg caattaaatt tgcccattaa
     1141 cctatttttc ataaaatgtc atttaaacaa gttatttatt aaaattcact ttattacata
     1201 aattatacaa ttaaaaagtt tcttcaaatt gtaaagatgc attaatcgag ttataatcat
     1261 aatgattaag atggtagatt tagtcttata taactacatt tactaagatt taaatagatg
     1321 agtacataat gtataaattg ggaatataca gtatgactta cggtaatata tcaatatatt
     1381 gatatattat ttgcacatgg gagtatgcta ttttttatct atacatctta aaaaaattta
     1441 cagttttaca attcaatatc attaaacaaa caacatattc agcttatttt tcattctctt
     1501 acgttacaag ttgtaaatac catcgcgtta aacaacaata gccaacgcta ataaaatata
     1561 ttgcgttaaa gtggagtgat taatatgtca aacgtactaa cagaaataga tagtcaatat
     1621 ccatatatga ctaaaaatga aaaaaagata gctaaattta tactaaattc acctcaaaag
     1681 gtgattaaaa tgaggtctca agacttagca agcttattag atattagtac atcatcagtc
     1741 attcgattca gcaagaaaat tactgatggc ggttttcacg acttgaaaat aaatatatct
     1801 aaatatgttc ctaaagcatc atcaatttac aatgttgaat taatgaataa cgaaagcaca
     1861 gaatctttaa gaactaaact ccatacacgt actacacgcg cacttaatca tgcaaataac
     1921 gaattaaatg ataaaactat tgatcaaata tgtcattgtt taaaacgttc tgagacgatt
     1981 tttatatatg gttttggcgc atcttttgta gttgctaccg acttatacca aaagttatca
     2041 agaataggtc ttaatattca acttgttcaa gaaacacata tttttgctac attattagca
     2101 actcacaatt cgaacgacag tgttattctt attactaaca atggtacgca aagtgaaatg
     2161 caatcaatgg ttaaagttat tgatgactac catataccta taattacaat tactagtaca
     2221 agggataatc ccgtagcaca ggcatcaaat attgttttaa cttatgggaa aactgatgaa
     2281 aatgagatgc atatgggagc aacaacttca ttatttgctc aaatgttcac tattgatatt
     2341 ttatattatc gttatgttgc attaaattat cacgcatcat tagatttcat aacacaatct
     2401 aaaatggctt tagataacta tcgtaaacat ttatcaaata ttaactttaa acattaacag
     2461 tcttatataa attctttaat catgtcatat aataatttat gcatcatttt caaagcttac
     2521 atgtctttta tcttgtataa catacgcata tgaccaatga tttgataagg ccattcaatt
     2581 tcttatatta aaaacaaacg cagttgatta acggattcat tccatatatc aattgcgttt
     2641 gttattttaa taatgctatt ttttatttta aagcccacag taaaaactaa cgataatatt
     2701 gccacttata agtttttcac actacaactt cgatttaagc tatatttata aaaaaatgcg
     2761 cctactcata acattctttg gctatttaac tttaataagc tagggccaag tttgtcataa
     2821 gataagcgca cattataatc atactatttt gcttttacat tatttttatt atatctaata
     2881 aaataataaa tcgttacaat cgttaagaag atgacaccaa taagcactga tattctagtt
     2941 tcaacattaa ataccatacc aaccaatact aatattaaga ataagattgt taaatagtta
     3001 gttactgcgc caccaggcat tttgaatggg tgaccttcca attcttcagg gtgtaatttt
     3061 ctaaaacgta aatgactgaa taaaatcata aaccaaggaa tcatacctgg taaaatagac
     3121 gcactataaa cgtatacgaa aatactcttc gctccatcga tatataaagg taaaattacg
     3181 tttaataaag caccaattaa aataccaaga gatactgcta caacagtgta caatggtaca
     3241 ccatttctca tgattttagt aaatattttc ggcatttcac ctttatgagc aagtgtataa
     3301 atcatacgac tcgcactgaa aatacctgag ttacatcctg acattgctgc agttaataca
     3361 acaaagttaa ttaatccagc tgcaaatgta ataccgattt tagcaaatgt tgcaacaaac
     3421 ggacttccaa tgtctcctaa ttgattccaa ggatacactg aaacaataac aaaaatagcg
     3481 cctaaataga aaattaaaat tctccagata acaccattca ctgctttaac gatatttttc
     3541 tgaggatctt tagtttcacc tgccgtaata cctatcagtt ccacaccttg gtatgaacca
     3601 attacaattg ataatgcaaa gaagaaacca acaataccat ttggcataaa tccgccattt
     3661 gtccatagat tagaaatacc taccgcatgg ccaccatttc caaaaccaaa gaaaataaga
     3721 ccaaaaccag caataatcat taaaataatt gttacaactt taattaaagc aaaccaaaat
     3781 tcaaattctc caaacgcttt taccgagaat aaattcgctg ccattaataa taaaatagca
     3841 ataacaccag gaatccaagt tggcaattcc gggaaccaaa acttcatata ttctcctact
     3901 gcgatgacct cactcatacc aactacaatc cattggaata tattactcca tgctgtcata
     3961 taacctgctg caggatgtat ataatcactt gcaaatgttg caaatgatcc tgttgtaggg
     4021 tttaaataaa tcatttcccc cattgctctc atgattaaaa ataaaaagat acccgcaatt
     4081 aaatatgcaa ggataactga tgggcctgtc catttaattg tacttgtcgc acccatgaaa
     4141 agccccacgc caatcgtacc accaagtgca atcatttgaa tttgacgggc actcaatccc
     4201 ctttgtagtt cattgttctc cataacttct taccccatct ctgattgata ctaaataaat
     4261 ccataaaaaa acttattgcc gtaattatac tataaattat cgattttggt acattaatat
     4321 ttgcatacca tgcaatcgtt tacagaacta ctatagcagg cttttgcaac tttgcaaaca
     4381 attattttgt gatttttcta aaaaattaaa aaagttgctc aagtaccgtt gagtcacata
     4441 caacttcagc tattcttggt aattatgata taattttaat aatattaaaa aggagtatat
     4501 caatgttaaa acaaatactg ctacgtgcta caaaaatatc ggtactcttt gctgtagcat
     4561 ttttcatcat aaattatatc ggtatggaaa aacctgacat cctatattta gttggaagaa
     4621 ctattattgc aacacttgct ttcatactca tttgtcttac agtatttact attattaatt
     4681 ctccagagcg caagattaaa ctcggtacga ctttaccgat tgcattaatt attggtatca
     4741 ttttcggcgc catattttta acggtacaaa tcggtgtcat cactgggtta atcatcggtg
     4801 tgattgctac gtttatttgg gaattgattg aaaaaaataa aggaggtcgt tcatcatgac
     4861 aacagcatta attggtgtaa ttgtattaat cttactaatt attagctttg tgccaaacta
     4921 ccgtgctatg aaatcagcaa aagaacaagg aagtaatgca acaagagcta caattatggt
     4981 aggcatagat attatcttaa ttgtacttat catcgtgacg ttattattaa aatatatgta
     5041 actatttaaa ccaccctatc tcatacataa acatgaacgt tctaccattt cacatacata
     5101 tggtcgtcca tttaatgagg taaggtggtt ttctcgttaa agtttacctt gtaatagtaa
     5161 aaaatagcca attgttgcga gtattaagta aatcattgaa ccaacaacaa aagatcttac
     5221 ttgcacgcga aatggtgtct gtttgttaaa tataggacca atcaccaaat ttccaacgcc
     5281 taaaattact ggaccagcta gaaaaaacaa tataatgtaa cccatcatga tacgcctcct
     5341 atttcgtaaa gaaacgcgct acaatttgat cgtaatcaat atcttttgct acataatcta
     5401 cattagaaaa gtcctgtgcg cttgtcattt tattcgtatt aacaataaca tctaatccag
     5461 ctgcaatcgc ggctgttgca ccatttacag aatcttcaat agccaaacat tgtgtcggca
     5521 tataatttaa ttgttgtact gcagataaat ataattcagg gtcaggttta acttgttcaa
     5581 catcttctct accaacaact acctctatat aatcgtctag acctaatgct ttaaaggtgg
     5641 gcattatttc cgctttcaca ctacttgtgg caattgccat aggaatgtga cgttgcttta
     5701 aaaatgccat caatgattta atcgtatcaa tcatcggtaa tgttgtacta gtaagatgat
     5761 gttcttcata aagtttatct ttattttccg aaccaatcgc tttaattaaa tggtcatgca
     5821 aatctgtagc tgctcctcca atagaagaac gataaaaatc aatgcttata ggatcggcat
     5881 tatgcatctc taaatgttta ttaataacat taaataaatg ttgttccgta tctattattg
     5941 ttccatcgaa atcaaatata actgctctat acatatattt actcctctat tttttctctt
     6001 tatcaaagct taacatagtc cgatgaccac ttcattctat tagtgcaatt ataacttttt
     6061 tgaccaatac tttgctaaaa taggccctgt tatattatga atgaaactaa acactgcgcc
     6121 tggtactgct gcaattggat taaaatgcaa tgctgctaat gacacagcta aaccagagtt
     6181 ctgcattcca acttcaatac tgactgcttt ttgatcttgt cgatctaatt ttaaaagacg
     6241 agctaaccaa tatccaatcg tataccctaa tacgttatgt aaaataacta ctaaaaatat
     6301 taataatcct gtagttaaga tttggtgctt actgccacct acaactattg ctaaaattaa
     6361 tgaaatagca acaactgata taattggcaa agctgtagaa gctttttcag caattttacg
     6421 attaataatt tgcaaaacaa tacctaaagc aattggaatt aatacaactt gaacaactga
     6481 ccacaacata ctcacgaaag atacttccaa ccattcattt gcaaatagat atattaacgc
     6541 aggtgtaacg aatggcgcta gcaacgtaga gaccgtcgta atagaaacag aaagtgctac
     6601 gttagctttg gctaaataac tcattacatt acttgatgtc ccacccggac aacatccgac
     6661 taatattacg ccaacagcaa tatcaggtgg taaatgaaaa gactttgcaa ttataaatgc
     6721 taatgtgggc ataatactga attgtagaca gacaccaata attactgctc taggtgcttt
     6781 aaagaccatt ttgaaatcat taggtgtaat tgttaatccc atacctaaca taacaatacc
     6841 aagtaaataa ggtacccatt tacctaatgt cgcaacatgt tgtgggaaaa taaatccaat
     6901 aacagctgct actaacatcc ataccaaaaa actcttcgtg gcaaatatac tcaatttttg
     6961 aaacattgtt aacacatcct cgtcgataat attgaaaaat attaccataa gaccagtaag
     7021 gttacaatta cttttctgaa aattaagatt atttagtcat tctcttctta ttgcaaatat
     7081 tttatcactg ataaacaatt attttaaact tcattcaaaa gtcttagtga ataaagaaaa
     7141 aaagcgtggt aaaatcaact tgaacaaaat taagtcacct ttataaatat taaacgtgta
     7201 tttaatgcca tgtattcctc gggaggtcct cacatgttaa ttgataaatt cgaaacatac
     7261 atcatcaaca tcgccggttt aaatgaccgt actactagaa aaaaattaag taagttatgc
     7321 aaaagtgtac aattttgtga tgcgttgcaa ttctcaataa acaaacagtt taatcagtat
     7381 gtattagaaa ttagcttacc aaagcaacaa cttccatact tcatttcatt tttaagtttt
     7441 catcagtata gtattttcca agtactttca ccaaagaaaa taaatgaatt acttgattct
     7501 gataatttat atcaatctgc aaagcgtttc gatatcaata ttgatggctt gcaagatgct
     7561 ttcattaaag acaaagtcat cgatattatg aatatgttcc aaaatcacac tgatatcact
     7621 tatacgttga ataagtctca tgcacatatt atatgtacgc cagaaatatt tgctaaattg
     7681 ttacatacga ttgcaactag aaatatcgac attctatctg ccaattatag atcgtcatct
     7741 atgagcaaag ctcgtatatc ataataaaag acacatctat acgatgatca tttacaatga
     7801 ttgcgtatag tgatgtgtct ttttttgtat tatttatctt cggacgattg atcctcaacc
     7861 atttgttcaa atgcttcacg aacttgtggt actgtcatac ctactacaac ttggatactc
     7921 ttaccactct ttactaatcc gtgtgccatt tgttgatgcg tgaaatattc cgtatctgca
     7981 actttacttt catcataaac agttaagcgt aatcttgttg tacagttagt aacatctttg
     8041 atattttctt tgccgcctaa accgtcgagg taatatgcag ctttgtcttc gtattcatta
     8101 ccagatgaag cacgtttaga atctacgcta tcaccttttt tatttttgta atcttgctta
     8161 gaaaataatt taacttcctc ttctgttttc ttacgtccag gtaatggaat atcaaatttc
     8221 aaaattaaga atctgaataa gaagaagtaa atcgctacaa atatcagacc gataataact
     8281 tgtgcaacat aagtacccca atgttcttta cctaatggca accagtttgt tgaaataaag
     8341 tctaacaagc caccacccat gttacctaca acaccaaatg cgtacattaa agtatccatt
     8401 gatgctgcta aaactgcgtg taaaacaaat aagtatggtg cgatgaataa gaatgtaaac
     8461 tctaatggct cagtaatacc tacaacgact gctgttaatg tggcaggaat aagtagtgcc
     8521 gccacctttt tacgattttc tttaggtgtc gtagcataca ttgctaaagc aatacctaaa
     8581 catccaaata ctttcccatt tccttgaagc ataaagccat aagggaattg ctcttttaat
     8641 ggcttagtac tcttagcaaa ctcatttaaa tgttgtaacc attcagcttt caagccgtga
     8701 ttgactacta ctggtccaac ttcaattggt gcataaataa agtgatgtaa tcctgttggt
     8761 attaatacac gttctaagaa gtgatacaac catacaccga tatagcctga agcaatgata
     8821 aagtgttgca ttgaaccaat accgtgctga acagttggcc aaataacaca agtaattgct
     8881 gctaatggta acatcacaaa aaatgaaatc gtaactacaa atgttaaacc ttggaataca
     8941 ccaaccattt ctggtaatcg tttactatag tatctattgt gaatccaagt aataatagct
     9001 gaaataataa taccacctag aatattcgta tccaatgtgg caatacctgc aattgatttt
     9061 aaaccaggta cattttcaac gcctttttct aaattagcgc caaacgtatg tggccattgt
     9121 gttaaaatgg catttataaa tgtattaaac attaagtaac ccatcaatgc tgcaagtgct
     9181 gcatgacctg gtgctttttt agctaaagaa agtggtagac ctacaacaaa tacaacttcc
     9241 atatgcgtaa aaataaccca gccaccagat tctattactg accaaaattt aaaccaaaac
     9301 gtatgttgat ctgctaaact ccccatgatt gtaggatttt taaataatgt cgcaaagcca
     9361 agcactatcc caaagaaagc gaacattaat accggtacaa tcattgcact accgaaacgc
     9421 tttatcgcat tcatcttcta ttccctccat atcatctttc ctaacaatac atctaattag
     9481 attcatttat aaatagatgt cttactattt aaatataata tatagtaaac gcttacacac
     9541 ctacaacgac attgacgtat tttgaaagta ttttgtataa tcagattatc ttttcatata
     9601 gtgaaaattt tttcacgacc ttatatatga catcgttgta tttgtaatac attcgtttta
     9661 aacgcataat caaacctata tcaatacaca aatatatata atgacataca agattttaat
     9721 gtaataacga tctattacac atttattttc aaggaggttg aatatgtttt tagatgaaca
     9781 cattaatcga aactttgata aacttaatga taatgattta catatcgctc actttatcaa
     9841 tacacatata gatgaatgta aaaatatgaa aatacaagat ttagcgcaat tcacacatgc
     9901 ctcgaatgca accattcaca gatttacacg caaattaggt tttgacggtt atagtgattt
     9961 taaatcgtac cttaaatttg aaagtaataa gacacaccaa ttaccttcta actctatcga
    10021 tagtttcaaa caagaaatcg atagtacatt cagttattta gaacgcgttg attaccagct
    10081 tcttactaaa aaaatgcata cagcttcaac aatatatttg tatggtacag ggcgtgcaca
    10141 gttaaacgtt gcttcagagg cacagcgtat attgttaacg ttacataatc atgtcattgt
    10201 acttcatgat gaacatgaac ttaaaatgat tttgaacaag agtgaaccta atgatttatt
    10261 ctttattatt tcattatcag gtgaaacaca tgaattatta gaagtaacaa acttgcttca
    10321 acttagacaa aaatacttta tttctgttac tacaatgaaa gataataccc ttgctcaaaa
    10381 agcaaattac aacgtctatg tatccagtaa cacattctac ttaactgatg gtacagacta
    10441 ttctagcttc atcagttatc atatattctt tgaaacactt gta
//
LOCUS       DAKTGS010000103        10351 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.103, whole genome shotgun sequence.
ACCESSION   DAKTGS010000103 DAKTGS010000000
VERSION     DAKTGS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.103"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(146..1300)
                     /locus_tag="O0286_002059"
     CDS             complement(146..1300)
                     /locus_tag="O0286_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0170424.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            complement(1517..2422)
                     /gene="yut"
                     /locus_tag="O0286_002060"
     CDS             complement(1517..2422)
                     /gene="yut"
                     /locus_tag="O0286_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCY0170425.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            2682..2984
                     /locus_tag="O0286_002061"
     CDS             2682..2984
                     /locus_tag="O0286_002061"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCY0170426.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            2998..3408
                     /locus_tag="O0286_002062"
     CDS             2998..3408
                     /locus_tag="O0286_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCY0170427.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            3405..5120
                     /gene="ureC"
                     /locus_tag="O0286_002063"
     CDS             3405..5120
                     /gene="ureC"
                     /locus_tag="O0286_002063"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCY0170428.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            5133..5585
                     /gene="ureE"
                     /locus_tag="O0286_002064"
     CDS             5133..5585
                     /gene="ureE"
                     /locus_tag="O0286_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCY0170429.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            5578..6267
                     /locus_tag="O0286_002065"
     CDS             5578..6267
                     /locus_tag="O0286_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCY0170430.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            6280..6894
                     /gene="ureG"
                     /locus_tag="O0286_002066"
     CDS             6280..6894
                     /gene="ureG"
                     /locus_tag="O0286_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCY0170431.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            6894..7730
                     /locus_tag="O0286_002067"
     CDS             6894..7730
                     /locus_tag="O0286_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCY0170432.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(7919..8266)
                     /gene="sarR"
                     /locus_tag="O0286_002068"
     CDS             complement(7919..8266)
                     /gene="sarR"
                     /locus_tag="O0286_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCY0170433.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(8694..9020)
                     /locus_tag="O0286_002069"
     CDS             complement(8694..9020)
                     /locus_tag="O0286_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0170434.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
     gene            complement(9084..9827)
                     /locus_tag="O0286_002070"
     CDS             complement(9084..9827)
                     /locus_tag="O0286_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0170435.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            complement(9853..>10351)
                     /locus_tag="O0286_002071"
     CDS             complement(9853..>10351)
                     /locus_tag="O0286_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0170436.1"
                     /translation="MSDINKVSELLTNYTKFRGLIMHMSIISESSKEINLTRLSIFIF
                     IINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPAALLNENSDAIRLSCKGLQQFSH
                     AKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKANFIERNIYDHSLMVSSNSIVHLK
                     YHLEI"
BASE COUNT         3528 a         1745 c         1593 g         3485 t
ORIGIN      
        1 tagctagtgt tatttatttt ttattttcgc aaaaaattta acaaaatatc ttaacatccc
       61 tataaatccc cacaaaaaaa gggtaaatcc gaacgtcaaa tttcatctct ttatgccaat
      121 caacgatcga tttacctttt aaaatttaaa atgtatctgt aatactctta gcaatatttg
      181 tatatgctgc atcttccata ggtggattgt gtaatcctgc acgcatgtca cgatagtaac
      241 gttgtaatgg tcgattcatt tctaagcttt tagcaccaac tattctcatc gctaaatcaa
      301 tcacttcaag cccttgattc attaccatca ctttactcgc acttgttgga tttcttatct
      361 gactgtcatc tttatattgt tgataccctt ttgctgtact ccataaaaat tgtctcgcac
      421 ataacaatag cgtttccatt ttccctaaat tttgttgcac agttggcaat gttgcaatcg
      481 ttccttcaat actattaggg ctatgttgga ttgcaaagtc tacagcataa tttctagcag
      541 cttgtgctat acctaaataa cagcttggta tatgcaatat ccaaccattt ggcgctttac
      601 ttttctctgt ttccactaaa tgttttaatg gtacttttac atcatttaat attaaatcat
      661 gactttctgt cgctcgcata cctaatacgt cccaattatc ggcaatttca acaccaggca
      721 aattcctgtc tactaagaaa aaaccaacac tttctaattc ttctatataa gcagcaacaa
      781 taatatgtgt tagtgcttta ctcattgatg tatatgtctt cacaccattt aaaatatacc
      841 catcatcagc tttaacagca tgtgtacttg gtcttccccc tcttgttgga ctacccattt
      901 cagcttcact aactgctcta ttaactaatg caccattatt aatttcaaca gcaaattgct
      961 ccaacatatc ttgagaccat aatttctgtt cataaatttg tcctacgaca ctcacatgcc
     1021 aaccaataga taatgctgtg gcaccatcaa gttcgcctaa aaatgattgc aaaataacca
     1081 tgtcttctat ggtcgcacct tcaccaccat atgcttttgg taacgttaat tttccataac
     1141 cttctttaat taaccattca atatttttat atggaaattg gctatgttga tcattatatt
     1201 cagctttagc cttaaatgta tctctaatta ctttgaattt ttcaatccat tttctttgaa
     1261 tttccgttgt tattaaaaag gaattatcca tatcattcat attagtcacc ccacacaaca
     1321 cacaatataa attatttatt cctagtttaa cactcgactt taaaaataaa aaggaacgtg
     1381 ctgtgaacaa ttaaaagaaa actgataatt cgattaagta tgcacttaat tttatccata
     1441 aaaatacacc ttaaactata caattcgctg tatagtttaa ggtgatattc aaacaatatt
     1501 aggtttgatt taactatcaa gcatctactt tgtcatgttt aataccagca aataataaaa
     1561 tccatgtcac gataataaat ggcaatgtta atgcaggtaa gccaaacggt tcaagcaatg
     1621 ttgttgtacc tagttgcacc actactgtta ataacacacc taaaaaagtc gaaatataag
     1681 ggttaatcgc tgttttaaat gtatatccta gtgcgattgc cattaataca aagttataac
     1741 cgaataatcc ctgattaata tcatcataat taccacctaa tagagctaca atgataaagc
     1801 ttaacaaact agctataaca gctaagagtg ttgcttttct tgatgctatc aatatgccga
     1861 ttaaaataca tacgccacca attacactcg cttcgataaa tacttgactg aatccttcaa
     1921 ataatgactg aatgaaatgt attctatcat tgttgctaaa attaaccgtt tcaatgtttt
     1981 gaggcattaa ctttaacgat gtatctacaa atttaacctg tcctgaaagt aaaattgtaa
     2041 accaagtcac aatgacaaaa ggcatcgtca gcatcggaac tttatatggt cttaaaactt
     2101 ctctcactgc agcagcaact ggtaacgtta ataaagttgc tatcattgtt ataacaatat
     2161 ctaatcctga cttatctaaa aagattgtta acgcaatggc agttagcact ggattaaatc
     2221 cagctaaccc atcattaatc tctgcctcac tataatttat aaaacgcgca aaagtatagg
     2281 cgatgatgct acctacaata gccgctaatc caattgtcca atcggctaca aataatccta
     2341 ttaagataaa taatcctgtc catttattac taattaacac aacctgagat atatttttca
     2401 aaagcacatc tattatcttc aaatctttca cctttccttt gaatcattac tcattaatat
     2461 actactattc tatgaaaagt tatattaaaa actattttgg tttttacata aatatattcc
     2521 actatttgtt ttcgattaga ttgtttgctt caatatcgga tatgctaaaa atataatttt
     2581 ctaattacac attgaacttt gtatattact tgtttatcac aacaattagg attaatataa
     2641 attttgtaaa ttgtatctaa tatgaaattg ggggttttaa tttgcatttt acacaacgag
     2701 agcaagacaa attaatgatt gtagtggcgg cggaagttgc acgtcgtcgt aaagcacgtg
     2761 gtttgaaact aaatcatcct gaggcattag ctttaatcag cgatgaatta ttagaaggtg
     2821 cacgcgatgg taagaccgtt gcagagttaa tgagttatgg tagacaaatt ctaaacaaag
     2881 aagatgtcat ggatggtgtc gaacacatga ttacagatat cgaaatcgag gctacgttcc
     2941 ccgatggtac taagttaatc acagtacatc accctattgt ttaaaggagg cagtcaaatg
     3001 ataccaggag aaattattac aaaaagtaca gaggttgaaa ttaataacca tcatcctgaa
     3061 acagttatcg aagttgaaaa tacaggagat cgaccaattc aagtgggctc acattttcat
     3121 ttttatgaag caaatgcagc attagatttc gaacgtgaaa tggcatatgg aaaacattta
     3181 gatattccag ctggagcagc tgttcgattt gaacctgggg ataaaaaaga agttcaatta
     3241 gttgaatatg ctggcaaacg taaaattttt ggttttcgtg gtatggtcaa tggtcctatc
     3301 gatgagtcac gtgtctatcg cccaactgat gaaaatgatg aatatgcagg tgtattcgga
     3361 gataacggtg ctgaaaacgt gaataaaaaa ggaggaaaaa gatcatgagc tttaaaatga
     3421 cgcaaaatca atatacgagc ttatacggtc caactgttgg agattccatt cgtttaggtg
     3481 atacgaatct atttgctcaa atagaaaaag actatgcggt ttatggtgaa gaagctactt
     3541 ttggtggtgg taaatctatt agagacggta tggcgcaaaa tcctcgtgta acacgtgatg
     3601 acgtgaacgt tgcagacctt gtcatttcta atgccgttat tatcgattac gataaagtgg
     3661 ttaaagctga tataggcatt aaaaatggtt atattttcgc cataggtaat gccggcaacc
     3721 cagatataat ggataatgtc gacattatta taggttcaac aacagatatc attgccgctg
     3781 aaggtaaaat cgtcactgct ggtggtattg atactcatgt tcattttatt aatcctgaac
     3841 aagcagaggt cgcattagaa agtggtatta cgactcatat tggtggtggt actggtgctt
     3901 cagaaggttc taaagcaaca actgtaactc caggtccatg gcatattcat agaatgttag
     3961 aagctgccga aggtttaccg attaatgtcg gttttacagg taaaggacaa gcaacaaatc
     4021 caactgcact cattgaacaa atcaatgccg gagcaattgg attaaaagta catgaagact
     4081 ggggtgcaac accatctgct ttgagtcatg cattagatgt tgctgatgaa tttgatgttc
     4141 aaattgcatt acatgcagat actttaaatg aagcaggatt tatggaagac acaatggctg
     4201 ctgttaaaga ccgtgtactt catatgtacc atactgaagg tgctggtggc ggtcatgcgc
     4261 ctgatttaat taaatccgct gcattttcaa atattttacc ttcatctaca aatccaactt
     4321 tgccttatac acataatact gtagatgaac atttagatat ggtaatgatt actcaccatt
     4381 taaatgcggc tattcctgaa gatatcgcat tcgcagattc acgtattcgt aaagaaacga
     4441 ttgcagcaga agatgttctg caagatatgg gtgtattcag tatgattagt tccgattcac
     4501 aagcaatggg ccgtgtaggt gaagtaatta cacgaacatg gcaagtagca catcgcatga
     4561 aagaacaacg tggtccttta gatggtgatt ttgaacataa tgataataat cgcatcaaac
     4621 gttatatcgc taaatataca attaacccag caattacaca tggtatttct gaatatgtag
     4681 gatctatcga gccgggcaaa ctagctgaca ttgtcttatg ggacccaatt ttctttgggg
     4741 ttaaacctga attagttgta aagggcggat taattaactc tgccgtaaat ggcgatgcaa
     4801 atggttctat acctacatct gaaccgatga agtaccgtaa aatgtatggt caatacggcg
     4861 gaaaccttac aagtacgtca atgacattcg tgtctaaaac tgcttatgaa aatggtatca
     4921 atcgtgcatt aaatttaaaa cgcatggtgc gtccagttaa aaatattaga caattatcta
     4981 aagcagatat gaaaaataac agtgcaacac ctaaattaga cgttgatcca caaacatatg
     5041 aagtatatgt agatggagaa aaaattacaa gtaatgcagc aactgagtta ccattaactc
     5101 aaagatactt cttattctag gaggaacata gaatgattgt tgaagaaatc caaggcaata
     5161 ttgccaattt gtcaaattca gaaaaacaaa agcacgtcga aaaagtatac cttgaaaatt
     5221 cagatcttgt taaacgtatt caaagagtcg ttacagacca tggcactgaa ataggcattc
     5281 gtttaaaaca acctattgac ttacaatatg gagatatttt atacgcagat gaccataata
     5341 tgattattgt ggatgttaat tcagaagatc ttttagtgat tcaaccaaga acattgcaag
     5401 aaatgggaga tatagcccat caattaggaa atcgtcattt gccagcacaa tttacagaga
     5461 ctgaaatgct agtacaatat gattatttag ttgaagattt attaaaaagt ttaggcatcc
     5521 catatgttcg agaagaccgt aaagttaata aggctttcag acatataggg cattctcatg
     5581 attgatcata cacacttaag attatttcag ttctgtgatt cacagtttcc aacaggtgct
     5641 ttcagtcatt catttggtct tgaaacatat attcaacgaa atataattca tgacgatcat
     5701 acttttattg cttggttaaa aatgttttta caagagcaac ttacctattc tgatggttta
     5761 gccatgcgtt tagtttatga tgcattggaa aatgatgata cacaaaaagt attacacata
     5821 gataaactga tgtttgttca aaacttacct aaagaaacac gtgttggtgc aaaacaaatg
     5881 gggactcgca tggttaaatt ggctttagaa ctttataata gcccatggat tgcttggtat
     5941 catcaacaaa tgcaagataa gaaagcaaag ttaaatccag ctatttgttt tactatgcta
     6001 ggccatcatt taggtgtaga tattgaaacg attattgatt actatttata tcaaaatgtt
     6061 tcaagtttaa ctcaaaatgc tgttcgtgct attccacttg gacaaactgc tggtcaaaag
     6121 atagttactc acatgattcc ttatatagaa ggaacaagaa agcagatttt cgaattaaaa
     6181 gaagcagatt ttggcatgac agcgcctggt ttagaactaa atcaaatggc gcatgagaac
     6241 gtcaatgtta gaattttcat atcataggag gttataattg tggcaaatcc gattaaaatt
     6301 ggtattggtg gtcctgtagg tgcaggtaaa acacaattaa ttgaaaaagt tgtaaaacgt
     6361 ctttcaaaag aaatgagtat cggcgttatt acaaatgata tatatacaaa agaagacgaa
     6421 aagatattag taaattcagg agttctacct gaaagtcgta tcattggtgt tgaaactggt
     6481 ggatgtcctc atactgcgat tcgtgaagat gcatctatga actttgcagc aatagacgaa
     6541 ttattagaac gtcatgacga tatagaacta attttcatag aatctggtgg cgataactta
     6601 gcagcaacat ttagtccaga acttgttgac ttttcaatat atattatcga tgttgctcaa
     6661 ggtgaaaaga ttccacgtaa aggtggtcaa ggtatgatta agtcagattt ctttgtaatt
     6721 aacaaaactg atttagctcc ctatgtaggt gcatcattag aacaaatggc tgaagatact
     6781 aaagtatttc gtggtaaacg tccatttact tttactaact taaaaaccga cgaaggtttg
     6841 gatgaagtta tcgattggat tgaacgcgac actttactca aaggattatc ataatggatg
     6901 aacaacaatg gactgggcaa cttgatttaa cagtgttttt cgatggcaat cgatcagtat
     6961 caagagatat tttctttgaa aaagcactta aagtgatacg tccagtttat ctaaatcaat
     7021 ctaccattcc tacattttat atagtaaatg taggtggtgg ctatttagat ggagatcgtt
     7081 accgtatgaa tgtgaatgtc gaagataacg ctaaagtgac attgacatct caaggtgcaa
     7141 caaaaatata caagacacct tccaatcatg ttgagcagta tcaaactttt aatttgaaag
     7201 ataacgcata tttagaatat gtcgctgatc caatcatcgc atatgaaaat gctaaatttt
     7261 atcaacacaa tacgttcaat ctcaataatt ctagttcatt attttatact gatattttaa
     7321 ctcctggtta ttcaaaaact ggcgaagcct tcaagtatca atatatgcat ttaataaatg
     7381 aaatttatat tgaagatgag ttagtcacat atgataattt attattgaat cctaataaac
     7441 aatcgatcaa tgaaataggt tatatggaac attactctca ttatggctct gcttacttca
     7501 tacacgaaga tgttaaccaa aaactaatcg attcagttta tgaaaccatt agttcttata
     7561 gcaatacatt cgattgtcgt gttgctattt ctcaattgcc tacacatggt tttgcagtta
     7621 gaatttttgc ttatcgcaca caaattatag agaaaatact tggaaccatc caaagctata
     7681 tcgcagaaaa tatttatgat cgtaaacttg attttctaag aaaatattaa aataaaaaga
     7741 ctagtgtacc ttgtttcaag cttaataagc tgaaataagg tatggctagt ctttaacttt
     7801 aattaaagca tcttcaatat ttaaatttta agttaatttt atgtcccaat gctgaataaa
     7861 taacttcgtt ttaataaatt gttatcgttc aatgttatta aacgcaatta accttgattt
     7921 aatttttaat gtattcttct aattctgaaa tcagtttttg aatatttgct ttttgtgtat
     7981 ctgtaacata aacaataact gttctttcgt cttgtaaact tcttttcttt gataacaatt
     8041 ttaaatcttt tagcttttgt aaagctttag ttaaatagta aggtttgaac tctgagcact
     8101 tagcaatctc tttagatgag atttcgttag actcacttct taaaatatga tttaaaatat
     8161 aaatttcttc atagttcaaa ttgaacttct tttttgtatc tctgaaaaac ttcttaactt
     8221 gaaatgttgc gttgactaaa tcattaatgt cattaatttt actcattatt aaaccactcc
     8281 tctgatgcac atcttgttgt agcaaggttc acatgtaatt tattaaatcg aaactctatc
     8341 caactatgtt ataaagttca ttctaaacaa ataagtataa aaattcaata tttttattac
     8401 tagaatatgg ttaaatacat ttatttctta taataatttg atgtttaaga tattttgcca
     8461 aattgaaaat tattagtctg aatattttgt atcttaactg atatgtaaac ttttaaaatt
     8521 ttaatctata tcaacgtgtt tgatttgata ttttttatat taattggatg gcttgctcac
     8581 aaaatttata aaatataaaa aaagaagagc tttaaatcat aacgcatgcc atttatcttt
     8641 aaatgcaatg agttatcatt caaactcttc ttatcaacct acatacattg acattatact
     8701 atcatatgtt gttttacata ttcaacaaac atttcaacgt tagcactttt aatatcatgc
     8761 attgggacat taccaatatt aaatttgaac attatagttt gaccatcata atctatcttc
     8821 tctatttcat tataactaat acttctataa taaaattggc cattcatatc tacattcata
     8881 attaatcttt catttgttgc tataaatgcc ccttcaaatt cattctcacc ttgaacttga
     8941 tactcaatta ttcctagcac tgacggaccc tttttttcag ttggaaataa atcatttgga
     9001 ttcacattat ctaaaatcat aatatccctc ccacttaaaa ctataaacta ttcttcatag
     9061 gtatatgaaa tacaagtgat taactattta ttattaagat taacttgtat tcccttttca
     9121 agataattat aacatgcttg aataagcata gtgactttag aaaacttttc tcgcttgatt
     9181 gttacaataa cacgcctttc atctaattca catctctctt ttttaactaa tcccttcaaa
     9241 attaaacact ttaaaatcgg gctaaccgca aatatcgggt gatggaattc accctgtaat
     9301 tctttaaccg tcaattgacc tttatgcaga tttaatattc ctaaaacgta taactcttct
     9361 aatgataatt gacacatatt taaataacac tttagattcc gcaataattc ttgtgacgac
     9421 aataaatacg atacatagtc acaactaaaa tcaaatgtat attgaccaaa ataacgttca
     9481 atatagtcac taagctcatt attcatatat tctattttat ctatatgcat tttattaatc
     9541 gaaatagtca atttacgttg atccctttga tctcttcctt tcaatagcca ttgatgattg
     9601 tttaaataac ttaacatttg aagaagggcc atcttagatt ggatttcttt catttttaat
     9661 aaactatcta ttgaagcgct attggaatca gaattatcat atttaactaa tagatttaat
     9721 agtttcactt gagtcaaagt gagatgaaat gaatatttca acatatcatt aatgactttc
     9781 aacattagta tgtgtgcagt taaacgctta actttaaatt tagacatctt taaaacctct
     9841 cttaaaccat gcctatatct caagatgata tttcaaatga acaatactat tgcttgagac
     9901 cattaatgaa tgatcataaa tatttctttc tataaaatta gctttccaat aactgtgttg
     9961 ttgcataata tcattcacaa gtacaccatt ttcggaagta tgattatctt tatctatact
    10021 taaaacaatt tgtttagttt tagcatggct aaattgttga agacccttac acgataaacg
    10081 tatagcgtct gaattctcat ttaacaatgc ggctggcaaa caaatgacac attgtacttc
    10141 atgtttgaac ttcgttacaa tcatcgtgtc attttgataa ataacaatcc ctcgtaattg
    10201 attaagtata tttatgataa aaatgaatat tgataatcta gttaaattta tttctttgct
    10261 cgattcactt ataattgaca tgtgcataat aagtcctcga aattttgtat aatttgtcaa
    10321 aagttcagaa accttattaa tatcagacat t
//
LOCUS       DAKTGS010000104        10190 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.104, whole genome shotgun sequence.
ACCESSION   DAKTGS010000104 DAKTGS010000000
VERSION     DAKTGS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.104"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(894..1088)
                     /locus_tag="O0286_002072"
     CDS             complement(894..1088)
                     /locus_tag="O0286_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170437.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            1198..1890
                     /locus_tag="O0286_002073"
     CDS             1198..1890
                     /locus_tag="O0286_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170438.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(2058..2207)
                     /locus_tag="O0286_002074"
     CDS             complement(2058..2207)
                     /locus_tag="O0286_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170439.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(2754..3070)
                     /locus_tag="O0286_002075"
                     /pseudo
     CDS             complement(2754..3070)
                     /locus_tag="O0286_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(3063..4046)
                     /locus_tag="O0286_002076"
     CDS             complement(3063..4046)
                     /locus_tag="O0286_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0170440.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            4472..5215
                     /locus_tag="O0286_002077"
     CDS             4472..5215
                     /locus_tag="O0286_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0170441.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(5442..5627)
                     /locus_tag="O0286_002078"
     CDS             complement(5442..5627)
                     /locus_tag="O0286_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0170442.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5632..5970)
                     /locus_tag="O0286_002079"
     CDS             complement(5632..5970)
                     /locus_tag="O0286_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170443.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(6216..6824)
                     /locus_tag="O0286_002080"
     CDS             complement(6216..6824)
                     /locus_tag="O0286_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0170444.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(7310..8209)
                     /locus_tag="O0286_002081"
     CDS             complement(7310..8209)
                     /locus_tag="O0286_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0170445.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(8308..9774)
                     /gene="msr(A)"
                     /locus_tag="O0286_002082"
     CDS             complement(8308..9774)
                     /gene="msr(A)"
                     /locus_tag="O0286_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0170446.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
BASE COUNT         3456 a         1575 c         1422 g         3737 t
ORIGIN      
        1 gcgtgtctat gcgtagacat ggagacactt cagaattatg gagtgattac atgagtttaa
       61 ctatttttct ttattcagtt ttaggaagtt tattagcaaa ttatatctac gaaaaatctt
      121 caaaataaat aactttttcg gttctacttt gtacacttta tctacctggt agacctaatt
      181 gttattgctt tacaggtcta aaattcgtcg ttataatcta gttaaatccc gtaagggcgc
      241 acttatacgt cggataaatc gacatgtgca aaaacgtaat cgaccttcgt cgattttttt
      301 atttcaaaat taaagaaacg gcttgagata ttccgagcag ttataaaaat taatttgcgt
      361 tgaatattgc tactacattc ttgaaaaagt gatgtagatt tatagcccat aacttttttg
      421 aattacatcg acgatacggc gtcgaaagaa aaataaatag accgtatgaa agaaaaagct
      481 acctcacttt atctggggta gcttttattt tttatgccct tattttgaat tttaagaggg
      541 cattttaaag atttaggggt gattcatata gttttatatc taaaacttat atatgctttt
      601 aaaacgcaaa tatgagccaa ataaatatat gctattttca aaaacaaaaa tttgagcaaa
      661 aacaataact atttttagac actgcccatt tacatgcaaa ttaaaaatta gcataaaatt
      721 ctttaaaact tccccacgtt tttctaaatt cctgtagggc gtttttatca aaaatcaaga
      781 caagaagaaa ctcgttcaca ctcgtttcaa aaaaacaaga aatctaatct atacacctac
      841 cgttttccaa ccccctcaaa gcatacaaat taaaaacaaa attattcata atcttacttt
      901 cttttaacaa aaacataaaa accataaata gacaaattaa ctatcaaagg caataaacag
      961 tataaaaaaa ccacgaataa atcttcatca acatctaaaa taaaaaagaa taaaacaatt
     1021 aacaaaatac taattaacaa acctaaaagt ggcaaacttt ttatatattt ttctaaattt
     1081 tcattcataa aatagactcc tttcaaaaat atgtttataa atatattaaa ataaatattg
     1141 attttataaa gcgcttactt atacaattaa aatatcttat tatcttggag gtatcaaatg
     1201 aaaattataa aattattttc aataatttcc ttgttcctac tggtatttag cgcgaatttt
     1261 tctaatgctt acgcaaatga agaacaaaaa agttctttac tagaaaatca aaaagaaaaa
     1321 ttgaaaagca aaaacatcga agtatcacaa atgaccaaga aagaaataag ttttctagaa
     1381 caattgcaaa ataaaaatga agatatgcaa aaattcgaaa agcaaatgaa aaaagaagga
     1441 tttaaaaaaa taaagctaga agacaaaaat tctaaattaa aatatctagc tacagaagca
     1501 aatgataaaa atgcatatgg ctacatagct aataacgctt ataaaaatga aaaaacaaaa
     1561 gaaattgtag tttctgaaac aatatatgat aactacaaca aaaaaatcac aaaattcatt
     1621 gccgaaaaaa gaagtatgac tgaaaacaaa gagggaaaaa tacttattaa caaaaattac
     1681 gttgaatata aggctgaaaa agttgataaa aacagaccgg taacatatgc aggattcaaa
     1741 tggaacggca aagcattcgc ttgtagtatg gctggacttt atgcatgtgc tcactactgt
     1801 ggagtatggg caatcgtaaa tccaatagcc ggcggtgtat gtgaagcagt ttgcggaact
     1861 gcatttgcag ctggatgctc tgtaggttga ttttattcca attgttttat tgacgttgag
     1921 cctcggaacc cttaacaatc ccaaaacttg tcgaatggtc ggcttaatag ctcacgctat
     1981 gccgacattc gtctgcaagt ttagttaagg gttcttctca acatcaataa attttttcgg
     2041 cataaatacg atgttcctta tttatcctca taaaaaacaa tagctgaaat atataaccaa
     2101 ttactgtgtg tttgaatttc taatacatga atatcattct ttaaaaacct attaacttgt
     2161 ttatctaatt gacgatgagt aaaagtagcg aaagttttaa tcttcatgat atctctcctt
     2221 ttataaaaat agcaatcctt attacaatat taaacacttt tattttggat tttaagaggc
     2281 attttgaaga cttagaggca aatcatatag gttttgtact taaaaatcta tacagtctct
     2341 taaaacgcaa atatgagcca aataaatata tgctaatttc acaaacaaaa atttcagcaa
     2401 aatcagtgac taatttttta gatactgccc atttacatgc aaatttaaaa attagcataa
     2461 aatactttaa aacttcccca tgtttttcta aattcctgca gggcttttct tatcaaaaac
     2521 ttagaaattt taatttaact ctaaaatctc cgtaagagca taagggaaat ttgctatcat
     2581 tcaaaaatga tagatctggc aaggccagac attccaaggg ttttaagtta ttttaaagtg
     2641 atatcataca aagctgtagt gtcacacttt tgtgacacta catacagcat ttgtcacaaa
     2701 aataagacag cactttttta cagaaaaaca ataaaaaaag acatcagtta tcactaatgt
     2761 cttaaataat aattttgctt ttcgaaattc caaattgcgg taattgaatg tgcttttttt
     2821 cttcgtctgt cgttttatct tcatcacttg tattaatcaa attaccatct tcggcatcgt
     2881 ctaagtttaa aattttatgc ttttgtttaa gcaatccacc ataacttaac atacgttttc
     2941 gatacaaacc tttttctaaa tcacttacaa tttcttgatt tttttcgtcg tcatcagtta
     3001 aaaagtcaga tgatttaacg gaatattttg atgtctcttc gagtgttata aaatcatttg
     3061 aattattgat taaagatatt atattgtttc atgtttttac ggcgcatata aagggcatgt
     3121 gtttcttcaa gttcagttag aattgtttgc attaaataag cttgcattga cttgattgtt
     3181 tccttttggc ctttaactcc cctatcatgt atagcttttt taagtctttt taaagaagtt
     3241 aatatttcat cctcaatgtc ctctaacatg taatgcatat cttcatgcct tgaattaaat
     3301 gatttttttg ctttaagaat aacagccttt atgatttcta aatctctata atcatatttt
     3361 aataaataat tagttagttg ttctgggaat tcttgcaata gcatttcttt atcatcaaaa
     3421 ttatgtgtaa attgatttga atgatttgaa tgattactaa tttcattttt gtactttata
     3481 tcattcaagt cattcatatc attattctca gtatcattaa agtcaatgtc attaaagtca
     3541 gtatcattag tgttcgattt tcgaacttct ggacgttcga ttttcgaact tctggacgtt
     3601 cgattttcga acttctggac gttcgatttt cgaacttcct tctcgccgta aggttcaaca
     3661 tcgttttctt ctttttctat tttgtaaata tcacgttctg tgacaatagg tttcattaaa
     3721 tatagaatat ttggtttatt taaaccacgt cgtttttgaa tgagtagacc atcgttttct
     3781 aacccttttt ttatttttgt taacttttct tttttacaat ttaagatttc cattagcttt
     3841 tcatttgaat aaacaaagta aatgttacca tcttcatcaa cccagctatt tttaatagat
     3901 aaatttaagc gatcacgtaa gatagcatag gctattttca tatcattagt tagattttta
     3961 taattttcac tggtaaagaa tactttaggg atttgataaa atctttcttt gtaattttct
     4021 tgcactgtaa aatattgatt ttgcatgaca aaaaacctct ttccatattt tagtttggta
     4081 agaggtaata aaacgttttt atttagcttt acatttattt aatgatttag gtgcttgcac
     4141 taagatatta ttcattatat aatacaagta tcgaaaatta tataaataaa cagtttctaa
     4201 acgttttatt accaaacccc gactattacc agtagttggg gtttttgtta tattaagttt
     4261 attatagcac tttaaataaa cttataaaag ggaaaatata gctttatcaa gggtttataa
     4321 acccttgata aatatcataa acctttcata aacccttgat aaaaaggttg atggatgttt
     4381 atttttattt attaatcact ttattattaa gcattataag cttataatat tttatcaagg
     4441 gtttataaac attttataaa gggttgatga tatgaaaagt gtgaaaaagt tgagtgaaga
     4501 attaggggtt agtaagcaaa cgatttttaa taatattaag cgattgaata tagagacaat
     4561 aaaacaggaa aacacttcgt ttattaaaga agataccgat atagaaaaaa tcattcaaag
     4621 agtaaatgaa aataaaaaga aatatggttt tgaaagtact actgaagaca aacaaaaaaa
     4681 agggtcggat aatattaatt ttgaatcgaa aagcgatgct caaatagtag aaatccttaa
     4741 aaatcagatt gatactttaa ataatcaaat tgaaaaacaa gaaagtcgtc atgaaacaac
     4801 aattgaattt tacagaaaag agttgcaaga aagatcaaaa ttgcttgaaa atcagcaagt
     4861 tctggcatta gagagtaata aaaagattca aaaattagaa aatcaattag aaaaagaaaa
     4921 acaccttaat tatccctttg atacatctac taacactaga caaaacgttg atgcacaaga
     4981 aaaaacatat actactaatc ctgtaaatat taacagagat caggaagaaa ctaaagaaac
     5041 agagattcaa tataaggata tatctggaag tcaaagcgac gaaagtacac aaggtgaaga
     5101 agcaaaaaaa gaagacgttc cagctaatct aaatgacaat gatagtgata tagaagaaaa
     5161 gagtgaagaa acggaaccta aaaaggggtt ttggagtcgt ttgtttggta gctaaaataa
     5221 ttgattaggc actaaaagtt ataaaatttt agtgtctttt atatagtgag aacttctggt
     5281 aatcatcgat tatgtaaact aaagtatttc aaataattaa ctgtattaat actctcatca
     5341 tgattgcgtt ataaacgttc gttattgaga caaaatttaa tagtactact acacaaaaag
     5401 agagtgcgaa ttcgcactct ctttttgtga taaatgaatg gttaagaaaa taattcatcc
     5461 acagttgtat caaacaatct tgccaactta aatgctaatg tcaatgtagg atcatattta
     5521 ttgttttcta ttgcattaat agtttgccta gacactttta attctttact taaagtttct
     5581 tgagaaattc ctttttgttt tctatattct ctaattctat ttttcattaa tttaaccttt
     5641 agatgtatat tttttatttg tagtatagta attaaacagc ataagaaata gtgctacaat
     5701 tataaagaaa atttctggat attgagaaaa ccaagatcct tcaatacttg gtgacaaacc
     5761 tgtaaattta agcactagtg taaataacat acaatataca atccaaataa aacttttagc
     5821 cagtatttca ataaacattt tacgttgtct ttcatcgtat cgatcttgtt gtcctttttt
     5881 atctaatgtt tctgaaggta aatatttgtt agttatagcc gtccataaac aagaaaaaat
     5941 taaaataata ataaaaggaa ttaaattcat tataacacct cataaatgta aaaagtattt
     6001 gacatttatg agtgtaaata tttttgatac aaatgtcaaa tactttttac attataatat
     6061 attttaaaac accttattag taaaacaatt actatataga aagctgcgaa ctcgtactct
     6121 tttttcttgt ataattatta aaatctcaat tcaataatat gttatggaat ttgagacact
     6181 gtgagagacg ctcatttgca ttgacaatca atttatcaag ataatccctt atcatgttta
     6241 attctataaa ctgtttgtct tgtgatatta acttctttcg caatcttact aatcgctttg
     6301 ccttcttcta acatttccac cacgcgatga taaataacac gtttctgtgg gtctttagca
     6361 tttggtgaat aaagtaaagg gcgcccttta tatacgcctt tttccttcgc aacttgaatc
     6421 ccttgcgctt gtcgacgttt actttcattt ctctcttgtt ctgaaaccat tgctaatatt
     6481 tgaatgatta aatctttcat aaacttatcc agtaatggat tgccaattac ttcattcatc
     6541 ataggtaagc tggtaatcat taactgaacg tctttttctt ttaaataatt cacagtttca
     6601 ataatttcat catagttacg tcctaaacga tcaatagatt ccacaacgaa tcgatctccc
     6661 attctgacaa aattgagtgc ttcttgaaat acaggtctat tttccactga ctttcccgac
     6721 tgtttctctg taaagatttt ctccacacca aacgtcttta aattatccaa ttgtctttct
     6781 aaattttgat ctatcgatga tactctcgca taacctataa tcattcatct tcactccata
     6841 ataaatatac accctaatca tacgttttaa ttgttataat ttcaacaatt ctttttgtat
     6901 ggttagggtg tactcaaagt gtacatatct ataattaaaa cttactaaaa ataaatatag
     6961 attttttctt tgtaacagaa ccgttcattt gaaggtcatc tctatcataa atatttaaat
     7021 taactaaaaa ttttaatcat ctgtttaata cttatgaaat gtatttacgg taggtattaa
     7081 aattgatgaa atttatataa gtgctttcaa tgaaaatgac cgtttattta tcctcctgat
     7141 ttaaggagtt agaaatttcg aaaacgattc tttccatttg ttgcatttgt tccatgtcca
     7201 gatatttaat acattataga aaaccaaaga ccatcactaa ttaaacgcag ttttaaaatt
     7261 tcatcaggat tgccttcgtc tttaaaatgg gcctctttta atataagttc tactctttca
     7321 tacctaactc tttcaatgcc atcgtttcat acgccgattc tcctgattcc atagcaaact
     7381 cagcgataaa cattgggaat actgcattga gttctattat atgctctttc attcgtggcc
     7441 atatttcacc tccagcttta ccgtatgctg ttataagttg ctctaatcct tcgtcgtcga
     7501 atacacgatg gtgtcctata aagtccattg atgggtcgga gtgggttgct tcagtccagt
     7561 ctatgagacc tgtgacgttt gcttggttat ctaccattat atgtcctgga tgtaagtccc
     7621 catgtatcat ggtcgcatgt cgaggccata gttcgtcgtt ttctaaccat tgtttccatc
     7681 tgttccataa ttcatctgat acgccataag tttctttaac tttattcatt cttctttgaa
     7741 agtcattttt tatttcttgt atggttttga tatttatatg ctgaacgtta atgttttctt
     7801 ctggtatgtt gtgtaaatct acgagtgttt cagctaatgt gttaataaag ttttctggta
     7861 atggtttgtg ttcaatttcc catacataat tttgtatttc tggatctatt gtggctgcgg
     7921 gtttacctgt aagttttggg taagcaataa ggtcttttgc atgtactttc cacttcggta
     7981 tttcaaatga aacattcttc tgtaagaagt ctaccgtttg tttttcgggt tttgttcgtt
     8041 tataaacgtc aggtcttctt ggtagtctca aaacccattc tactccatgt ttatcttttc
     8101 caaatgcaac ttgaaaatca agtcccgatt cattcaatga gattgtttga ggttggatgt
     8161 gtaattgata tttttctgca catttaataa tttcattatg tcgagtcatt ttattggatc
     8221 cttatttcta ttatattttt cttgtgaacg tattttttat attgatataa atgaacctaa
     8281 tattcaatat aaattagtgt atagttttta agttatatca tgaagagatt gtcctgttaa
     8341 ttcccatttt ttatctgata catgtttaac aaaccttgta tcatgtgatg taaacaaaat
     8401 gattccagga tatttattca taaacatttc taatgcttct aatgttttaa tatctaaaaa
     8461 attagttggt tcatccaaaa ttaacatatt cgctttcgtt gaaaataata ctgctaacga
     8521 taatttcgtt ctttccccac cactcaaaac attacaagaa cgatcaagtg cttcatttaa
     8581 acccaagtta tttaaaatag ctctactgaa tgattctgat gaatccgttt catccattaa
     8641 atattgcaat aatgaaacgt cacgcatgtc ttcataagca agttgacgat agtatgccat
     8701 ttgcacttta ggagaacaat caattccctc tatttggtgg taaatagctt caagtaaagt
     8761 tgtcttacct acaccatttg cacctacgag cgctatattt ttgccatatg gtatttggaa
     8821 tcgtacttgt gttagcagtt tttgacttcc tttaaccaat gttagatttt gtgcaatgat
     8881 tggataatta ttatggatat cataaatttt attttgtggg aaattgaatt catgataact
     8941 ttgtggtttt tcaacttctt ccaaatgttc cattcttttt tcaatatgct tagcttgttt
     9001 ttgagcagcc ttctcaaccg tgcctttttg ttttgatgca cttaaacgat ctggtgctat
     9061 acttttgttt ttttgttttg atgatgcttg tgccatttgt tgcgcttgat ttcgtttagc
     9121 tttactggct tgggacaatc tttgtttttc acttatatac tgttcatatt gacgttgttg
     9181 ttctaactgt tcttgttcat attgattttg atactgtgtg taattacctt taaacactct
     9241 tattgtgcca tcttcttgta tatcccaaat tgtgtcagca atttggtcta taagtgctct
     9301 atcatgactt actataatta aagtaccata gtaatatttt aaaatatttt tcagataatc
     9361 tttaccaatt ttatccaagt gatttgtagg ttcatctaaa agtaatatcg gactataatt
     9421 tgatatgaca ttagctaatt tatattttgc tttttcacca ccactcatac tgtcggttgt
     9481 atgcataggt atatggagtt cactcattaa actaccgtct aacgtgttaa aatcattttc
     9541 cacatccatg ttaagctgtt caaaatattg aatttcgcca accgtttcaa ctgttccaga
     9601 ttctggtttt gttttttgag caatcatatt tagtaatgtg gttttgccag taccattacc
     9661 cccaataagt gctatttttt caaattggta agcgtaaaga tgaccgatgt taagtgatcg
     9721 taaatctgtc aatttatgat tgatttggtt aaatttaatt gtatattgtt ccatatttat
     9781 acactccttg tatatttaaa ggacgtgtac gcaaaaaaat gagacaggct actcccgtct
     9841 cattcacatt acactttcaa acacttaaaa atttattatg atgttttagt atttgaaagc
     9901 gtttattatt cctatcgaag gataaaaaat gacataagtc atcctatgag tgtgtattgc
     9961 ggacaagcct ttttgaatta atttagtttt gtattcaaaa aggtttattt gagtctcata
    10021 gaagctgtca ttttaatcct ccttcatcga taaaatagta atgttaatat aacattttgt
    10081 tggttttttt acaatatatc tctaagtaga aagattatat aacaaaagta taaaaataca
    10141 attttagatt gattatacat ctccttaatt tagagtgtac tcataaaata
//
LOCUS       DAKTGS010000105        10177 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.105, whole genome shotgun sequence.
ACCESSION   DAKTGS010000105 DAKTGS010000000
VERSION     DAKTGS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.105"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            504..902
                     /gene="nrdI"
                     /locus_tag="O0286_002083"
     CDS             504..902
                     /gene="nrdI"
                     /locus_tag="O0286_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCY0170447.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            865..2970
                     /gene="nrdE"
                     /locus_tag="O0286_002084"
     CDS             865..2970
                     /gene="nrdE"
                     /locus_tag="O0286_002084"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCY0170448.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            3088..4059
                     /gene="nrdF"
                     /locus_tag="O0286_002085"
     CDS             3088..4059
                     /gene="nrdF"
                     /locus_tag="O0286_002085"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCY0170449.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            4573..4728
                     /locus_tag="O0286_002086"
     CDS             4573..4728
                     /locus_tag="O0286_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170450.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            4767..5738
                     /locus_tag="O0286_002087"
     CDS             4767..5738
                     /locus_tag="O0286_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170451.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            5725..6681
                     /locus_tag="O0286_002088"
     CDS             5725..6681
                     /locus_tag="O0286_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0170452.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            6678..7439
                     /locus_tag="O0286_002089"
     CDS             6678..7439
                     /locus_tag="O0286_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170453.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFRRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            7558..8586
                     /locus_tag="O0286_002090"
     CDS             7558..8586
                     /locus_tag="O0286_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0170454.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(8903..9217)
                     /locus_tag="O0286_002091"
     CDS             complement(8903..9217)
                     /locus_tag="O0286_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170455.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(9235..10158)
                     /locus_tag="O0286_002092"
     CDS             complement(9235..10158)
                     /locus_tag="O0286_002092"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCY0170456.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
BASE COUNT         3582 a         1471 c         1801 g         3323 t
ORIGIN      
        1 atttgttcaa gtttttctaa tttagtattg gtgcctagtt ggaacgtttt acgaacattc
       61 gattagaaaa tggcacttta aatcatagtg tgtcttatgt ataatgaaac acataatata
      121 gtgttggtga aacgaaaaag acacaatatc ttgtgttttg tatgcaaatg ctttatttat
      181 gaagaaatta catttaaaag taatttaaca cagaaattta atagttatta tcaattaata
      241 gtcatatttt tagaaaatgt actgagcaaa tggaagatat ccaatgatgt aaacactaca
      301 tatagtgatt tttatacatt caacccatat aagctactat tttctcaaat ataaatctat
      361 gcaattggtt tacatttgag aaaataagta gcttcattat agttaataca atgctgagat
      421 aaccatagta accatgttgt taaagcattt tttaattgga atgactactt tatttaaaag
      481 ggttgaagaa agaaggtgat ccaatgaaaa taatatattt ttcatttact ggaaatgtcc
      541 gtcgttttat taagagaaca gaacttgaaa atacgcttga gattacagca gaaaattgta
      601 tggaaccagt tcatgaaccg tttattatcg ttactggcac tattggattt ggagaagtac
      661 cagaacccgt tcaatctttt ttagaagtta atcatcaata catcagaggt gtggcagcta
      721 gcggtaatcg aaattgggga ctaaatttcg caaaagcggg tcgcacgata tcagaagagt
      781 ataatgtccc tttattaatg aagtttgagt tacatggaaa aaacaaagac gttattgaat
      841 ttaagaacaa ggtgggtaat tttaatgaaa accatggaag agaaaaagta caatcatatt
      901 gaattaaata atgaggtcac taaacgaaga gaagatggat tctttagttt agaaaaagac
      961 caagaagctt tagtagctta tttagaagaa gtaaaagaca aaacaatctt cttcgacact
     1021 gaaatcgagc gtttacgtta tttagtagac aacgattttt atttcaatgt gtttgatatt
     1081 tatagtgaag cggatctaat tgaaatcact gattatgcaa aatcaatccc gtttaatttt
     1141 gcaagttata tgtcagctag taaatttttc aaagattacg ctttgaaaac aaatgataaa
     1201 agtcaatact tagaagacta taatcaacac gttgccattg ttgctttata cctagcaaat
     1261 ggtaataaag cacaagctaa acaatttatt tctgctatgg ttgaacaaag atatcaacca
     1321 gcgacaccaa catttttaaa cgcaggccgt gcgcgtcgtg gtgagctagt gtcatgtttc
     1381 ttattagaag tggatgacag cttaaattca attaacttta ttgattcaac tgcaaaacaa
     1441 ttaagtaaaa ttgggggcgg cgttgcaatt aacttatcta aattgcgtgc acgtggtgaa
     1501 gcaattaaag gaattaaagg cgtagcgaaa ggcgttttac ctattgctaa gtcacttgaa
     1561 ggtggcttta gctatgcaga tcaacttggt caacgccctg gtgctggtgc tgtgtactta
     1621 aatatcttcc attatgatgt agaagaattt ttagatacta aaaaagtaaa tgcggatgaa
     1681 gatttacgtt tatctacaat atcaactggt ttaattgttc catctaaatt cttcgattta
     1741 gctaaagaag gtaaggactt ttatatgttt gcacctcata cagttaaaga agaatatggt
     1801 gtgacattag acgatatcga tttagaaaaa tattatgatg acatggttgc aaacccaaat
     1861 gttgagaaaa agaaaaagaa tgcgcgtgaa atgttgaatt taattgcgca aacacaatta
     1921 caatcaggtt atccatattt aatgtttaaa gataatgcta acagagtgca tccgaattca
     1981 aacattggac aaattaaaat gagtaactta tgtacggaaa ttttccaact acaagaaact
     2041 tcaattatta atgactatgg tattgaagac gaaattaaac gtgatatttc ttgtaacttg
     2101 ggctcattaa atattgttaa tgtaatggaa agcggaaaat tcagagattc agttcactct
     2161 ggtatggacg cattaactgt tgtgagtgat gtagcaaata ttcaaaatgc accaggagtt
     2221 agaaaagcta acagtgaatt acattcagtt ggtcttggtg tgatgaattt acacggttac
     2281 ctagcaaaaa ataaaattgg ttatgagtca gaagaagcaa aagattttgc aaatatcttc
     2341 tttatgatga tgaatttcta ctcaatcgaa cgttcaatgg aaatcgctaa agagcgtggt
     2401 atcaaatatc aagactttga aaagtctgat tatgctaatg gcaaatattt cgagttctat
     2461 acaactcaag aatttgaacc tcaattcgaa aaagtacgtg aattattcga tggtatggct
     2521 attcctactt ctgaggattg gaagaaacta caacaagatg ttgaacaata tggtttatat
     2581 catgcatata gattagcaat tgctccaaca caaagtattt cttatgttca aaatgcaaca
     2641 agttctgtaa tgccaatcgt tgaccaaatt gaacgtcgta cttatggtaa tgcggaaaca
     2701 ttttacccta tgccattctt atcaccacaa acaatgtggt actacaaatc agcattcaat
     2761 actgatcaga tgaaattaat cgatttaatt gcgacaattc aaacgcatat tgaccaaggt
     2821 atctcaacga tcctttatgt taattctgaa atttctacac gtgagttagc aagattatat
     2881 gtatatgcgc actataaagg attaaaatca ctttactata ctagaaataa attattaagt
     2941 gtagaagaat gtacaagttg ttctatctaa caattaaatg ttgaaaatga caaacagcta
     3001 atcatctgct ctgaattagc agatgattag actgctatgt ctgtatttgt caattattga
     3061 gtaacattac aggaggaaat tatattcatg atagctgtta attggaacac acaagaagat
     3121 atgacgaata tgttttggag acaaaatata tctcaaatgt gggttgaaac agaatttaaa
     3181 gtatcaaaag acattgcaag ttggaagact ttatctgaag ctgaacaaga cacatttaaa
     3241 aaagcattag ctggtttaac aggcttagat acacatcaag cagatgatgg catgccttta
     3301 gttatgctac atacgactga cttaaggaaa aaagcagttt attcatttat ggcgatgatg
     3361 gagcaaatac acgcgaaaag ctattcacat attttcacaa cactattacc atctagtgaa
     3421 acaaactacc tattagatga atgggtttta gaggaacccc atttaaaata taaatctgat
     3481 aaaattgttg ctaattatca caaactttgg ggtaaagaag cttcgatata cgaccaatat
     3541 atggccagag ttacgagtgt atttttagaa acattcttat tcttctcagg tttctattat
     3601 ccactatatc ttgctggtca agggaaaatg acgacatcag gtgaaatcat tcgtaaaatt
     3661 cttttagatg aatctattca tggtgtattt accggtttag atgcacagca tttacgaaat
     3721 gaactatctg aaagtgagaa acaaaaagca gatcaagaaa tgtataaatt gctaaatgac
     3781 ttgtatttaa atgaagagtc atacacaaaa atgttatacg atgatcttgg aatcactgaa
     3841 gatgtgctaa actatgttaa atataatgga aacaaagcac tttcaaactt aggctttgaa
     3901 ccttattttg aggaacgtga atttaaccca atcattgaga atgccttaga tacaacaact
     3961 aaaaaccatg acttcttctc agtaaaaggt gatggttatg tattagcatt aaacgtagaa
     4021 gcattacaag atgatgactt tgtatttgac aacaaataac aattaaatta aaagaccttc
     4081 acatgtaaag ggaaatagcg attcgtttcg tcttgtctcc tacatgttga aggtcttttt
     4141 ttatgtgtat ctaactcatt atgagtctga gtaagaaatc aatgctctaa gatgtacaat
     4201 gctatttata ttggcagtag ttggcggggc cccaacacag aagcaggcgg aaagtcagct
     4261 aacaatattg tgcaagttgg cggggcccca acatagaagc aggcggaaag tcagctaaca
     4321 atattgtgca agttggcggg gccccaacat aaaagcaggc ggaaagtcag ctaacaatat
     4381 tgtgcaagtt cgggcggggc cccaacataa agaaaaactt tttcctttag aaattatcac
     4441 ttccacatga gttttactca tgtattccta tttttaagta cacattagct gaggctaatg
     4501 ttaagaacca ctacttaatc aatcattagt agtttttatc atttccacta ttcccagaca
     4561 tcaaaatctt aagtgttcta ttttacttta agtaaacaaa atacacattc cgaaaaatta
     4621 aatttcagtt taattgcaaa tatcaataaa attgacacta aattatttga aaggctattg
     4681 aaattatggt caaaaaacgc tactattaat gagaaatatt atcaatgata atgattatca
     4741 ttaatttaaa gggagaaaaa tttgtaatga agtatttatt aaagggaaat attttgcttc
     4801 tattactaat attgttgaca attatttcgt tgttcatagg tgtgagtgaa ctatcaatta
     4861 aagatttact acatttaact gagtcacagc ggaatatttt attctcaagc cgaataccaa
     4921 ggacgatgag tattttaatt gctggaagtt cgttggcttt agcaggcttg ataatgcaac
     4981 aaatgatgca aaataagttt gttagtccga ctacagctgg aacgatggaa tgggctaaac
     5041 taggtatttt aattgcttta ttgttctttc caaccggtca tattttatta aaactagtat
     5101 ttgctgttat ttgcagtatt tgcggtacgt ttttatttgt taaaatcatt gattttataa
     5161 aagtgaaaga tgtcattttt gtaccgcttt taggaattat gatgggtggg attgttgcaa
     5221 gtttcacaac cttcatctca ttgcgcacga atgctgttca aagcattggt aactggctta
     5281 acgggaactt tgccattatc acaagtggac gctatgaaat tttatattta agtattcctc
     5341 ttttagcatt gacatatctt tttgctaatc atttcacgat tgtaggaatg ggtaaagact
     5401 ttactaataa tttaggtttg agttacgaaa aattaattaa catcgcattg tttattactg
     5461 caactattac agcattggta gtggtgactg ttggaacatt accgttctta ggactagtaa
     5521 taccaaatat tatttcaatt tatcgaggtg atcatttgaa aaatgctatc cctcatacga
     5581 tgatgttagg tgccatcttt gtattatttt ctgatatagt tggcagaatt gttgtttatc
     5641 catatgaaat aaatattggt ttaacaatag gtgtatttgg aacaatcatt ttccttatct
     5701 tgcttatgaa aggtaggaaa aattatgcgc aacaataata aaaaaataat gcttttaatt
     5761 gcagtaacgt tattaattag tatgctgtac ttatttgtag gtattgattt tgaaatattt
     5821 gaatatcaat tttcaagtcg tttaagaaag ttcatattaa ttattttagt aggtgctgcc
     5881 attgcaactt cagtggtgat ttttcaagcg attacaaata accgtctatt gacaccatca
     5941 ataatggggt tagatgcagt ttatttattt atcaaagtat tgccagtctt tttatttgga
     6001 attcaatcgg tatgggttac taatgtatat ttgaacttta tattaacact tataacgatg
     6061 gtgttattcg cactaatcct attccaaggt atctttaaaa tcggacattt ttcaatttat
     6121 tttatcttac ttattggtgt ccttttagga acatttttta gaagcataac aggttttatt
     6181 caactgatta tggatcctga gtcattttta gcaatacaaa gtagtatgtt tgctaatttt
     6241 aatgcttcta attcgaattt agttactttc tcagcagtgc tattagtaat cttattagtc
     6301 attacaattt tactattgcc ttatttagat gtattgcttt taggtcgtgc tgaagcaatt
     6361 aatcttggga tatcgtatga aaaattaacg cgaattctac ttgtaatagt ctcagtttta
     6421 gtttctgtgt caactgcatt agtaggacca attacatttt taggtttatt aactgtaaat
     6481 ctagcgcatg aactaatgaa gacgtatgaa cataagtata ttttaattgc gacaatttgc
     6541 ttgagttgga ttagtttatt tagtgcgcaa tgggtagttg aaaatgtgtt tgaagctacg
     6601 acagaaatga gtatacttat tgatttgatt ggtggaagtt atttcattta tctattagtt
     6661 agaaggagaa atgcgcaatg attcaagttg aaaatttaac taaaactata aataatcaaa
     6721 tgatattgga agatattagc atagatatcg aaaaaggtaa attgacttct ttaattggac
     6781 ctaatggtgc gggtaagagt actttacttt cagcgatatg taggttaatt cgttttgaga
     6841 acggtgaagt gaaaatagat ggacagctca tgtctgatta taaaaataat gacttgtcga
     6901 aaaaaatatc tatattaaaa caaacaaacc atactgaaat gaatattacg gtagagcagt
     6961 tggtaaactt tagacgattc ccttattcta aaggtcgttt gacgaaagag gatcatgata
     7021 ttgtcaatga tgcgctagat ttgttgcaac tacaagatat cagaaatcgt aatattaagt
     7081 cattatctgg tggacaacgt cagcgtgcat acattgcaat gacaatagca caagatactg
     7141 aatatatttt gctagatgaa ccattaaata atttagatat gaagcatgct gttcaaatta
     7201 tgcaaacgtt aaaaatgtta gcgcataaaa tgaataaagc gattgtcatt gtgttacatg
     7261 atattaactt tgcgtcctgt tattcagatc agattgtagc attgaaaaac ggacaactag
     7321 ttaagtcaga tttgaaagat aatgtcattc aaagtagtgt tttaagtgat ttatatgaca
     7381 tgaatattca aattgaacat ataagaaatc aaaggatttg tttatatttt aaggattgat
     7441 aatttggaga cactttaaag gggtgatgcg ccaattaaag aagggttaaa cgtaaagcat
     7501 ttatttatat ttcacatcaa gcacacagat taagccaaaa gaggagaata ttatattatg
     7561 aagaaaacag tcttatattt agtattagca gtaatgtttt tattagcggc atgcggtaac
     7621 aattctgata aagaacaatc aaaatcagaa actaaaggtt ctaaagatac agtaaaaatt
     7681 gaaaataact ataaaatgcg tggcgagaaa aaagatggta gtgacgctaa aaaagttaaa
     7741 gaaactgttg aagtaccaaa aaatcctaaa aatgcagttg tgttagacta tggcgcatta
     7801 gatgtaatga aagaaatggg cttatcagat aaagtaaaag cattacctaa aggggaaggc
     7861 ggtaagtcat taccgaattt cttagaatca tttaaagatg ataaatatac aaacgttggt
     7921 aatttaaaag aagtgaattt tgataaaatt gctgcgacga aacccgaagt aatctttatc
     7981 tctggacgta cagctaatca aaagaattta gatgaattca aaaaagctgc acctaaagcg
     8041 aaaattgttt atgttggtgc agatgaaaag aacttaattg gttcaatgaa acaaaacact
     8101 gaaaatatcg gaaaaattta cgataaagaa gataaagcta aagaattaaa taaagattta
     8161 gataacaaaa ttgcttcaat gaaagataaa acgaaaaact tcaataaaac tgttatgtat
     8221 ttactagtta acgaaggtga attatcaaca tttggaccta aaggtcgttt tggtggatta
     8281 gtttacgata cattaggatt caatgcagtt gataaaaaag taagtaatag caatcatgga
     8341 caaaatgttt ctaacgaata tgttaataaa gaaaatccag atgttatttt agcgatggat
     8401 agaggtcaag cgataagtgg taaatcaact gcgaaacaag cattaaataa tcctgtatta
     8461 aaaaatgtta aagcaattaa agaagacaaa gtatataatt tagatcctaa attatggtac
     8521 tttgcagctg gatcaactac aactacaatt aaacaaattg aggaacttga taaagttgta
     8581 aaataatttt aaaagagggg aacaatggtt aaaggtctta atcattgctc ccctcttttc
     8641 tttaaaaaag gaaatctggg acgtcaatca atgtcctaga ctctaaaatg ttctgttgtc
     8701 agtcgttggt tgaatgaaca tgtacttgta acaagttcat ttcaatacta gtgggctcca
     8761 aacatagaga aatttgattt tcaatttcta ctgacaatgc aagttggcgg ggcccaaaca
     8821 tagagaattt caaaaaggaa ttctacagaa gtggtgcttt atcatgtctg acccactccc
     8881 tataatgttt tgactatgtt gtttaaattt caaaataaat atgatagtga tatttacagc
     8941 gattgttaaa ccgagattgg caatttggac aacgctctac catcatatat tcattgattg
     9001 ttaattcgtg tttgcataca ccgcataaga ttgctttttc gttaaatgaa ggctcagacc
     9061 aacgcttaat ggcgtgcttt tcaaactcat tatggcactt atagcatgga tagtatttat
     9121 tacaacattt aaatttaata gcaataatat cttcttcggt aaaataatgg cgacagcgtg
     9181 tttcagtatc gattaatgaa ccataaactt taggcataga caaagctcct taacttacga
     9241 ttcctttgga tgttcaccaa taatgcgaac ttcacgattt aattcaatgc caaatttttc
     9301 tttgacggtc ttttgtacat aatgaataag gttttcataa tctgtagcag ttccattgtc
     9361 tacatttacc ataaaaccag cgtgtttggt tgaaacttca acgccgccaa tacggtgacc
     9421 ttgcaaatta gaatcttgta tcaatttacc tgcaaaatga ccaggcggtc tttggaatac
     9481 actaccacat gaaggatact ctaaaggttg tttagattct ctacgttctg ttaaatcatc
     9541 cattttagct tgtatttcag tcattttacc aggagctaaa gtaaatgcag cttctaatac
     9601 aactaagtgt tctttttgaa taatgctatt acgataatct aactctaatt cttttgttgt
     9661 aagtttaatt aacgagcctt gttcgtttac gcaaagcgca tagtctatac aatctttaac
     9721 ttcgccacca taagcgccag cattcatata cactgcacca ccaattgaac ctggaatacc
     9781 acatgcaaat tcaaggccag taagtgcgta atcacgagca acacgtgaga catcaataat
     9841 tgcagcgccg ctaccggcta ttatcgcatc atcagatact tcgatatgat ctagtgataa
     9901 taaactaatt acaataccgc gaataccacc ttcacggata ataatatttg agccatttcc
     9961 taaatatgta acaggaatct cattttgata ggcatattta acaactgctt gtacttcttc
    10021 atttttagta ggggtaatgt aaaagtcggc attaccacct gttttagtat aagtgtatcg
    10081 ttttaaaggt tcatcaactt taattttttc atttgggata agttgttgta aagcttgata
    10141 gatgtcttta tttatcactt ctcagtacat cctttct
//
LOCUS       DAKTGS010000106        10132 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.106, whole genome shotgun sequence.
ACCESSION   DAKTGS010000106 DAKTGS010000000
VERSION     DAKTGS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.106"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            243..1298
                     /locus_tag="O0286_002093"
     CDS             243..1298
                     /locus_tag="O0286_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCY0170457.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            1298..2194
                     /gene="murQ"
                     /locus_tag="O0286_002094"
     CDS             1298..2194
                     /gene="murQ"
                     /locus_tag="O0286_002094"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCY0170458.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            2206..3660
                     /locus_tag="O0286_002095"
     CDS             2206..3660
                     /locus_tag="O0286_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0170459.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            3663..4538
                     /locus_tag="O0286_002096"
     CDS             3663..4538
                     /locus_tag="O0286_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0170460.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(4732..5013)
                     /locus_tag="O0286_002097"
     CDS             complement(4732..5013)
                     /locus_tag="O0286_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170461.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            5226..8015
                     /locus_tag="O0286_002098"
     CDS             5226..8015
                     /locus_tag="O0286_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCY0170462.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            8220..9062
                     /locus_tag="O0286_002099"
     CDS             8220..9062
                     /locus_tag="O0286_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCY0170463.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            9064..9987
                     /locus_tag="O0286_002100"
     CDS             9064..9987
                     /locus_tag="O0286_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCY0170464.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            9974..>10132
                     /locus_tag="O0286_002101"
     CDS             9974..>10132
                     /locus_tag="O0286_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HCY0170465.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELIRD"
BASE COUNT         3641 a         1379 c         1995 g         3117 t
ORIGIN      
        1 ctgaataaaa tgtataaaag ttattgtaat aatccagcag tagcagctat tatcagcact
       61 taaaagaaag gataatcttt gatggtgtaa taattaactt gtgacagaaa tatgtaagat
      121 tattacaatg aacaggcttt aactgattaa tattgtaatg tgttttctct tatagtaaaa
      181 tgtaagcgat tacacaactc aactatattt tcctaataat tatattgtta aggagggcta
      241 ctttgacagg cttttcagtg tatttaggac aacctttaga tgaagcgtat attaagcgaa
      301 tgattaaaca aggttaccaa atgattttta catctgtaca aataccagaa gaagatgacg
      361 agacaaaata tcattatttc acaaaactac tcaatttatt aaaacatgaa caagtgactt
      421 acctcataga tgctaatcca tctattttaa caccatcttt ttatgaccat cttcgacaat
      481 atgatgcaca atttatgatt cgtatcgatc atagtacatc aattgaggca atcgaagcga
      541 taatggcaca gggtttaaag tgctgtttga atgcaagtat tatttcccgg gaattgttaa
      601 caagcttaca tcaacaattg aatgatttta cattactttc attttgtcat aactattatc
      661 caagaccaga tacgggatta tctgttgact tggtcaataa gaaaaatgaa ctcatttatc
      721 aatttaatcc aaaggcacaa atatatggtt ttattgtagg gagtgatttg cgaggtcctt
      781 tgcataaagg cttgccaaca attgaagcaa cgagacatag tcatcctgtc gttgcagcta
      841 aattattaca agaaactggt gtatctgaag tgttagttgg agactcattg attgaaatga
      901 ggcaggcaaa acaacttata gatttttgca agcataggca tttcacgtta tgtattgaag
      961 aagtgtttga tacgacagtg acttaccttt tcgatatgtg tcataaagta cgcccggata
     1021 atccggaaaa tgtcattcgt tcggaaacgt caagacaaat atgtccacat tcgattcaac
     1081 cacagtttac gacgcaacga cgcattggtt cagtaaccgt tgataatttg aataacggac
     1141 gttatcaagg cgaaatgcaa attgtgagac aaacgcttag tgcacatgac aatgtgaatg
     1201 ttgttgcaca aattattaaa gaagacttac cactgttaag ttgtatcgag ccgaatgata
     1261 catttgattt tcaaaaaact agggagtgta agaagtgatg gaaaatagta cgaccgaagc
     1321 gcgtaatgaa gcgacgatgc atcttgatga aatgactgtg gaagaggctt taattacgat
     1381 gaataaagaa gatcagcaag tcccgttagc agttcgaaag gcaataccac aattgacaaa
     1441 agtaattaaa aaaacaattg cacagtataa aaagggtgga cgattgattt atatcggtgc
     1501 aggtacaagt ggaaggttgg gtgtcttaga tgcagcggag tgtgtaccta cattcaatac
     1561 tgaccctcat gaaattatag gtattattgc tggtggacaa catgctatga cgatggctgt
     1621 agaaggtgcg gaagatcaca aaaaattagc ggaagaagat ttgaaaaata tagatttaac
     1681 atcaaaagat gtcgttatag gaattgccgc gagtggcaaa acgccatatg ttataggcgg
     1741 tttaacattt gctaacacaa tcggtgctac aacagtatct atttcatgca atgaacatgc
     1801 agttataagt gaaattgcgc agtatccagt agaagttaaa gttggtccag aagtattaac
     1861 tggttcaaca cgtttaaagt ctggtacagc acaaaagtta attttaaata tgatttcaac
     1921 catcacaatg gttggtgtcg gaaaagttta cgataacctc atgattgatg ttaaagcaac
     1981 caatcaaaaa ctgatcgacc gttcagtgcg tattattcaa gaaatatgtg ctatcacata
     2041 tgatgaagca atggcgttat atcaggtatc tgagcatgat gtgaaagttg cgacagttat
     2101 gggtatgtgt ggcatttcta aggaagaagc aacaagacgg ttattaaaca atggtgacat
     2161 tgttaaacga gcaatcagag atagacaacc ttaggaggga tttaaatgac caaagaacaa
     2221 caacttgcag aacgaattat tgctgcagta ggtggtatgg ataatataga tagtgtcatg
     2281 aactgtatga cacgtgtgcg tattaaagta ttagatgaga ataaagtaga tgaccaagaa
     2341 ctaaggcata ttgatggtgt catgggtgtt atacacgatg aacgcattca agttgtggtt
     2401 ggacctggta cagtcaataa agtggctaat catatggcgg aattaagtgg tgttaaacta
     2461 ggtgacccaa taccacacca tcacaatgat agtgaaaaaa tggactataa atcatatgca
     2521 gctgataaag caaaggcgaa taaggaagcg cataaagcaa aacaaaagaa tggtaagttg
     2581 aataaagtat tgaaatcaat tgccaatatc tttataccgt tgattcctgc atttattgga
     2641 gctggattaa ttggtggtat tgcagcagta ctgagtaact taatggtggc aggctatatt
     2701 tcaggtgctt ggattacgca acttataaca gtatttaatg tcattaaaga cggtatgtta
     2761 gcatacttag ctattttcac tggtattaat gcggctaaag aatttggtgc gacaccagga
     2821 cttggtggcg tgattggtgg tacaacgtta ttaacgggta ttgctggtaa aaatatttta
     2881 atgaatgtct tcactggaga accattgcaa cctggacaag gtgggattat tggcgttatt
     2941 tttgccgttt ggattttaag tattgtcgaa aagagattac ataaaattgt gccaaatgcg
     3001 attgatatta ttgtaacgcc gactattgca ttgttgattg taggactatt aactatcttt
     3061 atctttatgc cattagcagg ttttgtttca gacagtttag tttcagtagt taacggaatt
     3121 attagtattg gtggcgtatt tagtggattt atcattggtg caagcttcct accgttagtt
     3181 atgttagggc ttcatcatat ttttacgcca attcatatag aaatgattaa ccaatcaggt
     3241 gctacttact tattgccaat tgcagcgatg gctggtgctg gacaagtagg tgccgcatta
     3301 gcactttggg taagatgtaa acgcaacaca acattacgta atactttaaa aggtgcattg
     3361 ccagttggtt tcctaggtat cggagaacca ttaatctatg gtgtgacttt gccattaggt
     3421 cgacctttct taactgcttg tattggtggt ggtattggtg gcgctgtaat aggtggaatt
     3481 ggacatattg gtgccaaagc aataggccca agtggtgtgt cactattacc attaatctca
     3541 gataatatgt atttaggtta tattgcagga ttacttgctg cgtatgctgg tggattcgtt
     3601 tgtacatatt tatttggaac gacaaaggcg atgcgacaga cagatttgtt gggtgattaa
     3661 tgatgacaaa tattttatat cgcattgata agcagttgag tgattttacg aagacagaaa
     3721 agataatcgc tgattacatt ttaaagaatc cacataaaat cattgatatg actgtgaatg
     3781 atttggcaga tgttacgaat gttagtacag catcaattgt tagatttagt cggaaaatga
     3841 cacatcaagg ttttcaagag ctaaagattg cgatatctcg atacttaccc gaagatattg
     3901 caaccaatcc acatttagaa ttgattgaaa atgaatctgt agaaactttg aaaaataaaa
     3961 tgattgctag agcaacgaat acgatgcgat ttgtagctac taatattatg gatgcgcaaa
     4021 ttgatgcaat ttgtgatgtg ttgaaaaatg ccaggacaat atttttattt ggatttggcg
     4081 catcgagttt gactattggt gatctttttc aaaagttatc tcgtattggc ttaaatgtca
     4141 ggttattaca tgaaacgcat ttacttgtgt caacatttgc gacgcatgat gatagagatt
     4201 gcatgatttt tgtgacgaat caaggtagtc atagtgaatt gcagtcaatt gcacaggtgg
     4261 ccacacatta cagtattccc atcataacta tatctagtac agctaataat ccagtggctc
     4321 aaattgcaga ctatgcattg atttatggca gaactgatga aaatgaaatg cgtatggcgg
     4381 ctacaacgtc actatttgca cagttattca cggtagatat attgtactat cgatttgtag
     4441 cattaaatta tcatgcgatt ctagattgta taacccaatc gaaaatggca cttgataatt
     4501 acaggaagca tcttgcgacg atagatttta aacattagat attgtgtgcg ttctaacata
     4561 aagttagttg caacgtaaac aattgaagtt tttgatgtga ttcagttgct tgatgaaaaa
     4621 ttgttgctac aagtttgtaa aactggcata gaggctattt tgaaaatgat aaaggcgtga
     4681 caaattaaag caaagttcag atttgagctt tgcttttttc atagatggaa attaaatgct
     4741 gaagaataat tttaatgcta aatgaactaa tacgaaggtg ctaaaagcaa ttattatcaa
     4801 attagtgata tgaatgaaat gtttaaatcg tttgcgttga ttgtattcgg gacttttata
     4861 gagtaggtag tagtacataa agagtatggc tgcgaacatg ccgaatattg aagtgaagtt
     4921 acctacagtg actgccatgt aatgatttaa agttaaatag taacttagac taataataaa
     4981 aaataaaatg atttgatagg taattaacac cattcaagtc cctccattaa tcgtagacaa
     5041 aaaatttatc aatgatttga ttatttgtat tcaaatttta gtatacgact tacctcaaag
     5101 acaaatatta aaagataaga caataaaatt agaataaatt tacgtttgaa gataaaaaac
     5161 aaacattttt cattaaagtt tatgatatat ttagagcaat agaaagtgta tggaagggga
     5221 tatgaatggc ataccaaagt gaatacgcat tagaaaatga aatgatgaat caacttgaac
     5281 aattgggtta cgaaagagta acgatacgtg ataataagca attgcttgat aattttagaa
     5341 cgattttaaa tgagcgtcat gcggacaaat tagaaggcaa tcccttaaca gataaagaat
     5401 ttcaacgtct gttaacgatg attgatggaa aaagtatttt cgagagtgcc cgtattttac
     5461 gtgataaatt accacttaga cgtgatgatg agtctgagat ttatttgtcg tttttagata
     5521 cgaaaagttg gtgtaaaaat aagtttcaag tgacgaatca agtatctgtc gaggatacat
     5581 ataaagcacg ttatgatgta acgatattaa tcaacggact accccttgtc caagttgaat
     5641 tgaaacgtcg aggtattgat attaatgagg cgtttaacca agtaaaacgt taccgcaaac
     5701 aaaattacac aggcttattc cgctacatac aaatgtttat cattagtaat ggtgttgaaa
     5761 cgcgatactt ttctaataat gatagcgaac tattgaagag tcacatgttt tattggagtg
     5821 ataaacagaa taaccgtatc aatacattgc aatcgtttgc tgagtcattt atgagacctt
     5881 gtcaattagc taagatgata tcgcgctata tgattattaa tgaaacagat agaatactga
     5941 tggcaatgcg tccgtatcaa gtgtatgcgg tagaagcact tattcaacaa gcgactgaga
     6001 cagggaataa tggatatgta tggcatacaa ctggaagtgg taagacgttg acttctttta
     6061 aagcgagtca gattttatca cagcaagatg acattaagaa agttatcttt ttggttgacc
     6121 gtaaagactt ggatagtcaa acagaagagg aatttaataa atttgctaag ggtgctgtag
     6181 acaaaacttt taatacctcg caactggtac gccaactaaa tgataaaagt ttgccactta
     6241 ttgtaacgac gattcaaaaa atggctaaag cgattcaagg gaatgcccct ttattagaac
     6301 agtataaaac gaataaagtt gtatttatta ttgatgagtg tcatcgcagt caatttggtg
     6361 acatgcatcg tctagttaaa caacatttca aaaatgccca atactttgga ttcactggta
     6421 cgccacgttt tccagaaaat agtagtcaag atggtagaac aactgcagat attttcggta
     6481 gatgcttaca tacgtattta attagagatg ccattcatga tggtaatgta cttggtttct
     6541 cagttgacta tattaatact tttaaaaata aagctttaaa agcagaagat aacagcatgg
     6601 ttgaagcaat tgatacggaa gaagtatggt tagcggataa acgtgtggaa ttagtaacac
     6661 gacatatcat caataatcat gataaatata cacgtaatcg tcaatattca agtatattta
     6721 cagtccaaag tattcacgcg cttattaaat attatgagac atttaagcga cttaacaaaa
     6781 agttggaaca accgttaacg atagctggta tatttacgtt taaacctaat gaagatgatc
     6841 gtgatggtga agtgccatat cattcacgtg aaaaattaga gataatgatt agtgattata
     6901 ataaaaagtt cgagacgaat ttttcaacag acacaactaa tgagtatttt aatcatattt
     6961 caaaaaacgt taaaaagggc gttaaagata gtaaaattga tatcttaatc gttgttaata
     7021 tgttcttaac tggttttgat agtaaagtac tgaacacttt atatgttgat aagaatttaa
     7081 tgtatcatga tttaattcaa gcgtattcac gtacaaatag ggttgaaaaa gaatcaaagc
     7141 catttggtaa aattgtaaac tatcgtgact tgaaaaaaga gacagacgat gcactgagag
     7201 tattctcaca aacaaatgat acggatacaa ttttaatgcg cagttatgaa gagtataaaa
     7261 aagaatttat ggacgcttat cgtgagctta aaatgattgt gccgacacca cacatggttg
     7321 atgacattca agatgaagaa gagctaaagc gctttgttga agcttatcgt ttattagcta
     7381 aaataatatt acgtttaaaa gcatttgacg agtttgagtt tacaattgat gaaattggaa
     7441 tggatgaaca agagaatgaa gactataaaa gtaaatattt agctgtgtac gatcaagtaa
     7501 aaagagcgac ggctgagaaa aataaagtat ccattttaaa tgatattgat ttcgaaatag
     7561 aaatgatgcg taatgatacg attaatgtga attatattat gaatatattg agacaaattg
     7621 atcttgaaga caaagcggaa caacgtcgta accaagaaca aattagacgc attttagatc
     7681 atgcagatga tccgacattg aggttaaaac gagatctaat tagagaattc atcgacaatg
     7741 ttgtaccttc tttaaataag gatgatgata tcgatcaaga atatgttaat ttcgaaagta
     7801 ttaaaaaaga agcggagttc aaaggatttg ctggagagag atctatcgat gaacaagccc
     7861 taaaaacaat ttcaaatgac taccagtata gtggtgttgt aaacccacat caccttaaaa
     7921 aaatgattgg tgatttgcca ttgaaagaaa agcgtaaagc aagaaaagcc attgaatctt
     7981 tcgtggcaga aacaactgaa aaatacggtg tgtaatgatt cagccccctc gctagattag
     8041 tgtagggggc attattattt tctatctttt gttagttacc atgtgtttgg caaccatcat
     8101 ttggtttcaa tacaatttta taatcgtatg tgtatagaaa taatgtctat aggcatttaa
     8161 aatagtttat aatattaaca ggataagtat taaatgtatg ttgaaggagg tgcgtagcta
     8221 tggataatag aaatatgatt aatcgtgttt ttagtcaaaa gatattacat caaattgcaa
     8281 tcaaaaataa aagtgatgtt gttgatgagg catatgattt ttatatacag ggacctaaaa
     8341 atatcaatgt aatacagaag atgaaatctt tatataacta tcttaaaaag tcttatcgta
     8401 acgaatattt ttacaaaaac acaatactta ataaactcct tctaggacga cattctatta
     8461 atacaactac tgcactttct gagatgccca tagggaaaag tattgctgat tttatattgt
     8521 taaacggcaa aggcgttgtc tatgagatta aaacagaatt agataagctg gatagattag
     8581 ataatcaaat taatgattat tatgaagtgt ttaattatgt cgtagttatt acaaatgaca
     8641 aacatctgaa taaagttatg gctagataca aagatacaac agttggaatt ttagtgttaa
     8701 caactagaaa tacactgagt gaagttcaaa aacctaaaga aaacaatagt ctcttaaaca
     8761 caaaagcgat gtacaacttt ttacgaaaag aagaaagaaa aagagttatt gcacaaaatc
     8821 atatggatgt gccaacttat aatgatttca cagagtatga tgtgttattt gacgtgttta
     8881 aagaaatacc aatgacgaaa ctgcataaca atatgatttc tgagttgaaa aaaagaggaa
     8941 acatgaaaga atacaaagat gaatttttag cagcgccggc tgaaattaag ttcttgttat
     9001 atttcgcaaa aatgacaaag aaagataaaa ataaactata tcattttctt aaggaggatt
     9061 aatatgtatt atccttattt gcgtgggaaa caaaatgaac tatttgcaat tagagaattg
     9121 ttagagaaag gtttgattgg tgattgtatt caacctataa ttgaaccaat taaatataca
     9181 accacattta aaaatacttt gcaatactgt ggtgaaaaag cattctctat aaatttagta
     9241 gtaaattcaa agttaactga agaagagatt agtaacgaaa ctgttgcaca tttaactgaa
     9301 ataataacaa aaaacaaaag tgttattcaa aaagcttact tgggtccttc tgatgaaggc
     9361 aatgataggt tgaaacagca attttcaagt aatagtttag ctattttaac aagtgtagat
     9421 gattgggaaa tgtttggaga taaaaataaa cttgaaatgg tttttgtacc agatgataga
     9481 cacattaaac gtaaattgcg taatattcca aacaaaggca tcattatgga tccttttaat
     9541 aaactaagtc gtaatgttga ttatttagat aatgatgacg agttttatag cgacgatcac
     9601 ctttattata aggaagatgg atacgtagca ttttcagact attctgttat aggtggagaa
     9661 tatgtagacg gtggcttttc gccattagct attgcgatac atattgtcta ttttgatgag
     9721 gctaatgagc taagagttaa gcattttgtc tctgattcta ataatgatag atcaaatcca
     9781 ggtaaaaagt tttttgaggc tgtagataaa ttagtaacat ggtcaaaaaa cttagatatt
     9841 aaaaatagat cttatgcgct tggacaattt gaagaattaa atgaaaataa taagtatcca
     9901 ggattaggtt taattaaaag gttatctatc atgcatcacc tagaaattat gaatagatac
     9961 ttggagtctc aaaatgaaaa tatgtgaaaa atgttttaat aatactgaaa tcgtagaaat
    10021 cattgcaaat gataatagca aatttgacaa ttgtgatatt gataacaatc atcttggtgt
    10081 taaaatattt gatacgacta aggacataga taaattagaa cttattagag at
//
LOCUS       DAKTGS010000107        10007 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.107, whole genome shotgun sequence.
ACCESSION   DAKTGS010000107 DAKTGS010000000
VERSION     DAKTGS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.107"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..890
                     /gene="sasG"
                     /locus_tag="O0286_002102"
     CDS             <1..890
                     /gene="sasG"
                     /locus_tag="O0286_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HCY0170466.1"
                     /translation="KERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKG
                     ESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIE
                     EPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPIT
                     VNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNP
                     NNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLI
                     FSSIIGIAGLMLLARRRKN"
     gene            1274..2467
                     /locus_tag="O0286_002103"
     CDS             1274..2467
                     /locus_tag="O0286_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170467.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
     gene            2448..2681
                     /locus_tag="O0286_002104"
     CDS             2448..2681
                     /locus_tag="O0286_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170468.1"
                     /translation="MLRSLCKHYRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(2789..3097)
                     /locus_tag="O0286_002105"
     CDS             complement(2789..3097)
                     /locus_tag="O0286_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170469.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(3241..3507)
                     /locus_tag="O0286_002106"
     CDS             complement(3241..3507)
                     /locus_tag="O0286_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170470.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(3791..5599)
                     /locus_tag="O0286_002107"
     CDS             complement(3791..5599)
                     /locus_tag="O0286_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCY0170471.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            5716..6108
                     /locus_tag="O0286_002108"
     CDS             5716..6108
                     /locus_tag="O0286_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCY0170472.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            6110..8971
                     /locus_tag="O0286_002109"
     CDS             6110..8971
                     /locus_tag="O0286_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCY0170473.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQK
                     GKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKY
                     EDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTA
                     GYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
     gene            9277..>10007
                     /locus_tag="O0286_002110"
     CDS             9277..>10007
                     /locus_tag="O0286_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0170474.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEE"
BASE COUNT         3764 a         1286 c         1725 g         3232 t
ORIGIN      
        1 agaaagaacg taaatttaat cctgatttag caccagggac agaaaaagta acaagagaag
       61 gacaaaaagg tgagaagaca ataacgacgc caacactaaa aaatccatta actggagaaa
      121 ttattagtaa aggtgaatcg aaagaagaaa tcacaaaaga tccagttaat gaattaacag
      181 aattcggtgg cgagaaaata ccgcaaggtc ataaagatat ctttgatcca aacttaccca
      241 cagatcaaac ggaaaaagta ccaggtaaac caggaatcaa gaatccagac acaggaaaag
      301 tgatcgaaga gccagtggat gatgtgatta aacacggacc aaaaacgggt acaccagaaa
      361 caaaaacagt agagataccg tttgaaacaa aacgtgagtt taatccaaaa ttacaacctg
      421 gtgaagagcg agtgaaacaa gaaggacaac caggaagtaa gacaatcaca acaccaatca
      481 cagtgaaccc attaacaggt gaaaaagttg gcgagggtca accaacagaa gagatcacaa
      541 aacaaccagt agataagatt gtagagttcg gtggagagaa accaaaagat ccaaaaggac
      601 ctgaaaaccc agagaagccg agcagaccaa ctcatccaag tggcccagta aatcctaaca
      661 atccaggatt atcgaaagac agagcaaaac caaatggccc agttcattca atggataaaa
      721 atgataaagt taaaaaatct aaaattgcta aagaatcagt agctaatcaa gagaaaaaac
      781 gagcagaatt accaaaaaca ggtttagaaa gcacgcaaaa aggtttgatc tttagtagta
      841 taattggaat tgctggatta atgttattgg ctcgtagaag aaagaattaa aataattcat
      901 aatttaaata atagttgatt tgcattcact atatttagtt tgttaaaaac aacctagaat
      961 atgatgagaa tgatatacac cccaaaagtt ggattgaaag acctaacttt aaggggtgta
     1021 tttttgttat aaatttgatt tttttgataa tattttcata tttaatataa tcataattag
     1081 tagaatataa cctctgagta gtcattttgg gggttatgta tggtgtttca agtagtatgt
     1141 atatttcgaa ataattgata tttaactgac taaattatat tagaattaat tgcgctttgg
     1201 ataaagagag tttagaagaa ggtctaaaaa agctggtgga tgcattcaat tatttttatg
     1261 agaggtgata aatatgttgt atttagaagt tttgaaaaat agaaacttta catatttatt
     1321 gattggtaat ttcttacgtc ggagttgttt tgtgctgttt tcgttacaaa taatttggtt
     1381 tactgttgaa ttgacaaacc aatcatcttt aaaattatca atgatggtta tgagtcaaac
     1441 acttccattt attatatttg gtattttcgg tggcgcatat tctgataaac ataataaaaa
     1501 gaaaatactt tatctatctg atcttatact aagtttgatt ataataatta ttcctttact
     1561 tgcaataaca tcaaatttaa attatctgac gcttctaacc atatctacag ctatcaccat
     1621 tattaactgc tatacggatc ctgcgtttag ggcaattctt cctgaaatta ttgatgagga
     1681 gcatttagca acaagtaatg cgttaatcga tagtttgcaa agaggctcaa atattatttt
     1741 acctgcttta attggtgtca ttgtaatact agttggtaat gttggcgtct tttttatttg
     1801 tagtatattg ctatttttag gatttatttt taatgcactt ttaaaatata caaataacaa
     1861 catgattgat agacactcaa aagaagattt ttctgaaact tgggaattct tgaaacaatc
     1921 taaagaaatt ccatttatta taatcatcca atttgcctgc atattgatta atactggcct
     1981 ttggcgtgtt gtattaccat tgtttatttc caatatatta aaagagggcg ttggtgtata
     2041 tggactagct acatcatgtt taggtatagc atctttatta atgtcattga ttatgggttt
     2101 gctgtcagaa aagcgattaa tctttaaatt tagtattggt gttttagttt ggggaattgg
     2161 cttatcgatt attaatgtgt ttccaagtgt ggcgatatta tatataggtg caacattatt
     2221 gggacttgga caatctatag agggactaac aagatcagtt gccattcaaa taaaaacgcc
     2281 aaatcatttg atggggaaag tatttagtat ctcatctaca tcgaattatg cagcggatac
     2341 attgtcttta ggactgataa gtataattat acctttacta agcttaagta atatatttat
     2401 attaggaggt gtaattattt caatacttag cttagctggg ttaaagtttg ttaagaagtc
     2461 tatgtaaaca ttatagaatt ttaataaatg ctattaaggt gggaattgaa atgaaatata
     2521 aaattagttt ggcttataat ttagcaatta taatagggtc gctaattata ttatgtatac
     2581 taatatctag aggttatgat atttatgtaa tcttgattcc aattttgact attttagcta
     2641 gtttaatcaa tttgttttgt gatattaaaa agcataaatg attctatatt aatgcaactt
     2701 tgtattcgta caaggttgta tttttttcgc aaacaattac ttactgacag cgcatcaata
     2761 aatctaacca acacactatc acaaacattt atgggtattt tgtaatttta taaaagcaaa
     2821 cgtagaataa tgcgataagt aataatgcta tcgttaaaaa taagaatgct aatgcaataa
     2881 aatcatattc aaagcttaga attgaagaca ttaaaaatac tgcactcaaa cttatacata
     2941 gcgtaaatat atatgcaatt tggttcttgt gtttatctgc aatggccgtt tgatttataa
     3001 aagagattag tgtgaaatct ttagttattg cgacataaat gcctaatata accatactta
     3061 tgcagaatac gatactaaat acgatgttga cggtcattgt tgcaccttcc tgatagaata
     3121 taatgtactg tcttttagta atggatgcgt ttcttatata tgtatgtttc taaaaaacag
     3181 tacgttttta tcatttttta atttattagt tgaatgaatg gggttatgaa aaaggaagtt
     3241 ttaatctttt gtcatgaaat aaatgggatg aatatcacga ctagaagtaa tgttacgaac
     3301 aggagcgtat aaatctagag acgctaaatt cgacatagta tgttgctcaa ttatggctga
     3361 tgatgaattt aaagtatgtg cgttggaact gtcggatttt tgttcataat gttttgcata
     3421 ttgcgccatg atgaatagtg taaaaataaa taaaacaata agagatataa tgcccataat
     3481 caaaagtatt tgtttagagc ctttcattat ttcacatcct ttctaaaata tatttgtaac
     3541 taaatttaaa atagttattt ttgtaattct aaaccttttt catcgcgaaa acaattaaat
     3601 aggtcgcggt attaattatt atattattac cgcttaatat gaaaaataca tgaaaattaa
     3661 ttttctaata tacttttgaa aaattattac aaattagccc cttcaaaacg cgaaaacata
     3721 aggattctag tttcaaaagg gctgataagc ataaaatgaa atgtaatatt tcgatgtata
     3781 aaatttttaa ttagctaaaa atcatcgcat taattttttg agctacatca tcaaaattcg
     3841 gacattttaa cgacacatat aatttaattt taggttcagt accagaagga cgtaaagcga
     3901 taaatccttc gtcaaataag acacgaataa catttgattt aggagaatta atctgcgacg
     3961 ttgtatcttt atccaaatga taaacctcgc tagttaaata atcttcaatt gctttcactt
     4021 tgagtccttg aatctcttgc ggtggatttg aacggaattt ggtcattatt gcattaattt
     4081 tctttttccc ttcaaatcct tctagcgtat gcgagaataa tgtatcctca tgtctaccaa
     4141 cagtttgata aatctgttct aattcatctt tcaatgtttt gccatataat tttaactcag
     4201 aagcgtattt tataatgagt ggcacaattt gtacggcatc tttatcacgt acaaaaggct
     4261 ctgatagaaa accgtaactc tcttcaaatg cgaaaatcat attttgatga tcatccagtt
     4321 gtcttatttc ctgagcaata aatttaaagc ccgtcagcac ctctttgtat tcaacattat
     4381 tatagcgtgc taatgacttt gttaactcac tacttacaat agattgaatc attaagcggt
     4441 gacgtaattg agacgtttgt tgaatacgat aattaagtaa cagcgcacca atttggttgc
     4501 cattaaaata tgtgatatga ccgtgtgcat cacgttcagc aataccaagt cgatctgcat
     4561 cagggtcagt tgatattaat aagtctgcat gacttttatt tgcaagttcg actgcttgat
     4621 caaaagcgcg atgatcttca ggattagcac tttgaactga gctgaaattc ggatcaggtt
     4681 tacattgtgc ttctacaaga ttaaattgat tgaaattcaa agattgtaat aactcaggta
     4741 caatcggaac acttgtacca tgcaaactag tgaacacaac ttgtaaatca gatttaggaa
     4801 tatagccaat catattttga atgtgtttca tatagtcatc agttacagat ttcggaaaag
     4861 gcttgatata agatgtattt tgtttagaaa taggtatatc aatctgtaat ggatcgccaa
     4921 cttcttcgat ataacgactt gcaagctcag atgcatcagt cgataattgc gcaccatcag
     4981 aaccatatac tttgatgccg ttatagtctt tcggattatg acttgctgta atcataatgc
     5041 cagcagtagt attaagattg cgcaccgcga acgataattc cggtgtcgtt ttatatgtat
     5101 caggtaaata aactgttatt tgatggtttg ctaatacatt agcaattatt tgggcgaatt
     5161 cagttgaaag atgtctaata tcataatgaa tgactattgt tggactgttt gtttgggcat
     5221 ttaaataacg cgctaaacct aatgccaatt tttcaatagt aaacttatta agtcgacctt
     5281 caccaagacc gaattttccg cgtatacccg cagtaccaaa tgttaattta ctttcaaaac
     5341 cttctcgctg ttcaatgtca gattgctgct cataaaaatg tttaactaaa ctatcattag
     5401 ctcgttctat ccataattct ttatccattg ttgctaaaca tcctttcaaa atctcagtta
     5461 gacttaataa aacatgaaaa ctaaagccct tacatttatg taatgaatta taaagaaata
     5521 cgccccaaaa gtaaaaaaac acagccccaa gacaatactt ttcacaagta ttatataata
     5581 gatgtgtatg aaaatgcatg gagtagagcg ttcatgtgaa tgtaaaatat taatttttaa
     5641 tattgattta tatatagttt tgtaattttt gatagaataa caattaagat gattttaaat
     5701 tgaagggtga gtcttatgaa aaaagtaatc aatgtagtag gagctattat tttttctgat
     5761 aacaaaattc tttgtgcaca gagaagtgaa aaaatgagtc tgcctttaat gtgggaattt
     5821 cctggcggta aggttgaaaa gaatgaaact gaaaaagacg ctttgattag agaaattaga
     5881 gaagaaatga aatgtgattt aattgttgga gacaaagtta taactacaga acatgaatat
     5941 gattttggaa ttgttaggtt aacaacatac aaatgtactt taaacaaaga gttaccaact
     6001 ctaactgaac ataagagtat tgaatggttg tcaataaacg aacttgataa attaaattgg
     6061 gccccagcgg atataccagc cgttaataaa attatgactg agggataata tgagtagatt
     6121 actaaatgat ttcaatcaat ctttacataa agggtttata gacaaacata tttctcataa
     6181 agggaattat acaccgaagc ttttagtaaa taataaaaat gaaaaagttc tttcaacaat
     6241 tatagatgaa cttcaaaaat gtgaaacgtt ttatttttct gtggctttta taacagaaag
     6301 cggtttagcc agcttaaagg ctcaactatt agatttaagc aataagggtg ttaaagggaa
     6361 aatattaaca tctaattact tagggtttaa tagtcctaaa atgtatggag aattacttaa
     6421 attaaaaaat gtagaggtaa gattaactga tattgctgga ttccatgcca aaggatatat
     6481 ttttgagcat aaggattata gttctatggt aataggaagc tctaatttaa catctaatgc
     6541 gttaaaggtt aattatgagc ataatgtttt actgtctact atgaaaaatg gcgatttagt
     6601 tgatagtgta aaaagtgaat ttgacttgtt atggcaaaag agtaccccac tgactgagca
     6661 atggattaat tcatataaag aatcatttga gtaccgttcc ttagaaaaat tagcggaggt
     6721 tgaacaaact caaatgctgc tagctgataa ggtgaaaaaa tcagtggaaa ttgtacccaa
     6781 tttaatgcaa gcagaagcat taaggtcatt aaaagctatt agggataaag ccaaagataa
     6841 ggcgttaata atatctgcaa ctggtacagg taaaacgatt ttatgtgcat tagatgttag
     6901 agaagtaaac cctaataaat ttttatttat tgttcataat gaggggattt taaatagagc
     6961 taaggaagaa tttaaaaaag tattacctat aaaaaatgat agtgattttg gattgttgac
     7021 aggaaaacat agagatgtcg atgccaaata tttatttgca accattcaaa cattatctag
     7081 agatgataat tttaagcaat ttgatgaaaa tgaatttgat tatattgttt ttgatgaagc
     7141 acatcgttct gctgcatcta cttatcaacg agtatttaac tacttcaaac ctaagttcat
     7201 gctgggaatg actgctacac cagaaagatc agatgaatta agtatatttg aactgtttga
     7261 ttataatatt gcatatgaaa taaggttaca agcagcatta gaaagtgata ttttatgtcc
     7321 atttcattat tttggtgtga ctgattatgt acatcaaggt attaaagaag atgatgtaac
     7381 taaactgaga tatttaactt ctgatgaaag agttaattat attattcaaa agacagatta
     7441 ctatggatat tcaggtgaaa ttttacaagg attgattttt gtaagtagca aaaaagaagc
     7501 ttatgattta gcggataaac taagttcaaa agggattaaa agtgttgctt taacaggaga
     7561 tgatagtgtt aattatcgcc aaattgtaat agaaaaatta aaagaaggta agattaacta
     7621 tattataact gtagatcttt ttaacgaagg tattgatatt ccagaagtca atcaagttgt
     7681 aatgttgaga ccaacagagt ctagtattat attcattcag caacttggta gaggattaag
     7741 aaaaagttca aataaagagt atgttacagt aattgatttt attggtaatt ataagacaaa
     7801 ttatttaatt ccgattgcgc tttctgggga tcaatcgcaa aataaagata attataagaa
     7861 gtttttaacg aataacgatt cgattaatgg agtatctaca attaattttg aagaagttgc
     7921 taaaaaacag atttacaatt cattggatgc agtatcatta aatcaaaata aattaatatt
     7981 aaaagcttat gaagaagttg aaaatagatt gggacacatg ccgttactaa tggatttcat
     8041 acaacaacat tctatagatc caagcgttat attttctaaa tttagtaatt attacgagtt
     8101 cttagtgaga tataaaaaaa tagatacatt attgacggaa aatgaatcta aaaatctggt
     8161 tttcttttca aggcaaatag cacctggatt gaaaagaatc gatagtttag ttttagaaga
     8221 acttttaaaa aatgaattaa catatgatga attaaaaaat aaaatgttga acgaagttaa
     8281 ggatataaca gaagatgata tagatacttc attaagaatt ttagattttt cattttacaa
     8341 cgcaggaatt gaaaaaatct atggaagtcc cattatagaa cgtaatgaaa gaatgataag
     8401 actaagcgat gcatttacta atgctctaag taatcaaaca tttaatatgt ttttagaaga
     8461 tcttattgaa ttatcaaagt ataacaatga aaaataccaa aaaggtaaaa atggactgat
     8521 tttatataat aaatattcaa gagaagattt tagtaaaata tttaattgga ataaaaatgg
     8581 ttcgtctgta atcatgggat acatgataaa aagccaagaa atgccaatat ttatcactta
     8641 tgataagcat gaggatataa gtgatagcac aaaatatgaa gatgaatttt taagtcaaga
     8701 tgaattaaaa tggtttacta aatctaatag aacgctagaa tcaaaagaag ttcagaaaat
     8761 tttgtcccat agagcaaaag gaattaaaat gtatattttt gtacagaaaa aagatgatga
     8821 tggtatatat ttttattatt taggaactgc tggatatata gaaggttcag aaaagcaaga
     8881 taaaatgccg aatggttcaa acgtagtaac gatggattta gcactggata aagctgtaag
     8941 agatgatata tatcgttatc taacaaatta atgtaaaccg aaaaatgaat gttagtaaag
     9001 ttgtatttaa acatcaattt taataagtaa atacattgag tttcagtgtt attattttaa
     9061 atataagcgc aatttatctt aattaattag ttggttaggc caataaaaat tataaagata
     9121 tttgcgcttt ttaaataatt tgtgatgttg atatgtgaaa aatacaacta acaattaaaa
     9181 tccacgagga tttaatcata aaatttcctc taatattatt aatattggaa tttgaactta
     9241 actttgcatc aaaaataaaa atggggatgt gaaatgatga ttcactcaaa aaggttgagg
     9301 ctctggctgt atttagtatt attagctgtt tttatagggg cttgcggaat gaaaaaggaa
     9361 gaaagcagta aagataaaca aattaaagaa aacttcaaca aaacgttgag cctgtatcca
     9421 actaaaaatc ttgaagactt ttatgataag gaaggctttc gagatcaaga gtttgaaaag
     9481 ggcgacaaag gcacttggat aattcactct aaaatgacaa tagaaacaaa tggaaagaat
     9541 atggaatcca gaggattggt cctgtatgta gaccgcaata ccagaacgac aaaaggtgaa
     9601 tttattgtga gggaattatg ggaagataaa aaaggatact cacgtagtaa agaaaaagaa
     9661 tatccagtaa aaatggaaca taataaaatt atcccaacca aaccgatagc tgatgataaa
     9721 ttaagaaaag aaatcgaaaa ctttaaattc tttgtacaat acggtgactt taaagatatt
     9781 aatgattata aagatggtga catttcatat aatccgaatg tgccaagtta ttcagcaaag
     9841 tatcaattaa gtaatgatga ctacaatgtg aagcaactta gaaagaggta tgatatacca
     9901 actaagaaag cacctaaatt attaataaaa ggcgatggtg atttaaaagg atcatctata
     9961 ggacacaaaa atttagagtt tacatttata gaaaataaag aagaaaa
//
LOCUS       DAKTGS010000108         9918 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.108, whole genome shotgun sequence.
ACCESSION   DAKTGS010000108 DAKTGS010000000
VERSION     DAKTGS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.108"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(110..913)
                     /locus_tag="O0286_002111"
     CDS             complement(110..913)
                     /locus_tag="O0286_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0170475.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(971..1885)
                     /locus_tag="O0286_002112"
     CDS             complement(971..1885)
                     /locus_tag="O0286_002112"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCY0170476.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            complement(1887..2948)
                     /locus_tag="O0286_002113"
     CDS             complement(1887..2948)
                     /locus_tag="O0286_002113"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCY0170477.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(2954..4234)
                     /locus_tag="O0286_002114"
     CDS             complement(2954..4234)
                     /locus_tag="O0286_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCY0170478.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            4425..5807
                     /locus_tag="O0286_002115"
     CDS             4425..5807
                     /locus_tag="O0286_002115"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0170479.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(5861..6436)
                     /locus_tag="O0286_002116"
     CDS             complement(5861..6436)
                     /locus_tag="O0286_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170480.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            6767..7621
                     /locus_tag="O0286_002117"
     CDS             6767..7621
                     /locus_tag="O0286_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170481.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            complement(7764..8297)
                     /locus_tag="O0286_002118"
     CDS             complement(7764..8297)
                     /locus_tag="O0286_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0170482.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            8436..8624
                     /locus_tag="O0286_002119"
     CDS             8436..8624
                     /locus_tag="O0286_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCY0170483.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(8737..9339)
                     /locus_tag="O0286_002120"
     CDS             complement(8737..9339)
                     /locus_tag="O0286_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0170484.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(9336..>9918)
                     /locus_tag="O0286_002121"
     CDS             complement(9336..>9918)
                     /locus_tag="O0286_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0170485.1"
                     /translation="LIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVK
                     AELLAINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVL
                     SPTKQSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVK
                     SISQRVQHLNGTLAVDSTNGTKIIIEISTGGIA"
BASE COUNT         3233 a         1753 c         1358 g         3574 t
ORIGIN      
        1 tgtatttcaa atcctccata gtataactga agtcattttc acaaaaataa aaagccttaa
       61 ccacaagata tcttattcaa atatcatgtg ttaaggctta ttttaaaatt tataacaatt
      121 ttttgagcgc atgtggaata ccattttcat tatgatccaa cgtaacttca tttgcatatt
      181 gcttaagttc atctgatgca ttccccatag caatagctag acctgcgact tcaaacattg
      241 atttatcatt catactatca ccaaaagcaa tgacttgatc caccgaatat cccaatttgt
      301 gacataacgc tttaattgca ttaccttttg aaacgtcttt ggccataaat tctaagaaga
      361 atggcttact tgtcgtagca tctacattat cattgaacac accattcaaa tcaattctag
      421 cttctgtaat attcgctaca taatctacac ccatgacctt gggtacatcg ccttgaatat
      481 acgctttgat atcatcaaca cgtttcatcg gtaaacctgt taattctgct tcaatattca
      541 tatactcatg ttcgctgtcg taaataattt gaccatcatg atatgtaaga acgaaaaagc
      601 ctctatctcg acaataatct acaatttcat cgaaatcttg cttaccaatc gatttactta
      661 cttctacttc ttcattagtc atgttaatcg ttttactacc gttataacta ataatatagc
      721 tatgatgttc aggtaatttt aaatctctag cagttggaat cataccttca gtaggtctac
      781 cagatgctag aacaacataa taaccttcat cttgaattgc tgttaaataa tttgcagttt
      841 cttctgatat cacattatct gaatttagca atgtgtcatc catatctaaa acgacaactt
      901 tataatttgt catatcgcat taaatctcct ctctattctg catttaatac tattttacaa
      961 tataaagcgt ctattggtat acaattcgtt ctttaacacc actgatatta atatcgacca
     1021 attcagaatg gcatgatact acctgactat ttaaagagcg aaccaattcc ccactatttt
     1081 ctttacgact aaatattaaa atagttggtc cagcaccact aattacagtt gcataggcat
     1141 tatgttgact agcaattgtt ttcacttcat caaattcagc aattaaatgc tgacgatacg
     1201 gttcatgaaa gccatcttgt tgcatgagtt tacctgctaa ttcataattg tgctgtgcta
     1261 atgcacaaat cattgtatta ctaattgcac tacttttaac cgcttcacta tgtgttaatt
     1321 tttgtggtaa agcacgtctt gatgcttctg tttttagttc ataagttggt atcgttacaa
     1381 tcacatccac gtctggtatg tcgatatgtg caactgacgt ttctttcgag acatcattat
     1441 aatatccagc gattaaacca ccataaatgg tcggcgcaac attatcagga tgtccttcga
     1501 tttcagtcgc taattgtaat acctcatgtt tagacagttg gatatcacca aaataatttg
     1561 cgatatatat agctcctact aaagcagaag ctgacgaacc taaccctctt gccaatggaa
     1621 tatcacttct catttcgata cataaattag gcaagtcaac actatattta gaagcaactt
     1681 gttgtgctac atgataaata aagtttgttt cgtcagtagg caattgctta gatgcatcat
     1741 catggaaaat atattcccat tttgtccctg atgtttcctt tacagacaga tgcaaaaatt
     1801 tatctaaagc catacctata gaatcaaagc caactccaag gttggctgtt gatgcaggaa
     1861 ttgttaactc caaaacattc gacatgttat aaagctcctt taatataatc gataatgcta
     1921 tctttatcat ttggcaatgg ctttatcgga ttatctagta gtgaaatagc agtatcagga
     1981 tcttttaatc cattaccagt taaaatagca actacttttt taccttgagg taatttacct
     2041 tgtctatgca atttaattaa acctgcaata gaagcattac tcgctggttc actaaagaca
     2101 ccttctttag ttgtcattaa ctgatatgct tctagaattt catcatcagt aacactatct
     2161 attaatccat ttgattcttt aagagcatta gtcgccttat cccaactagc aggattacca
     2221 attcgaatag cagttgcaat cgtttcagga tttttaatga ctttattttg aacaattggt
     2281 gatgcgcctt cagcttggaa gccaaacatt ttcggcaatt gtgatccttt agcttcatga
     2341 tattctttaa agcctttcca atatgcagta atattacctg cattacctac aggaatcgct
     2401 aaaatatcag gtgcttcacc gtctaattgt tgtacaattt caaatgagcc tgtcttttgt
     2461 ccttcgattc taaatggatt gacagagttt acaagctcga tttcgccact ttttgcaatt
     2521 tcttttacaa tttctaaagc ttcatcaaag tttccttcaa tagaaacgat ttctgcacca
     2581 tacattactg cttgcgacaa tttacctaat gcaattttac cttctggtat tacgacgata
     2641 gcttttaaac ctgctctcgc tgcatatgct gctgcagacg ctgatgtatt tccagtcgaa
     2701 gcgcatatta caattttctt accttgctct tttgcttttg tcacagccat taccataccg
     2761 cgatctttaa atgaacctgt cggattggca ccttcatact tcacatataa atctatgcct
     2821 agtattttag acatattttc acaatgaatg agtggtgtat ttccctcgtt caatgttaat
     2881 tttggtgtat tttcatttac tggtaaatgt gctttaaact cttctactaa tccttgccat
     2941 cttctcatta taattaaact ccttctactg ggtatacttt gtcaacttcg tatccatgct
     3001 tagtgattaa ttcttccggt gatgattcca atccaactat aacagcggca tatgattgat
     3061 tatcgtaatt tgcaactctt aatgatcggt gaaatggtaa tattgccttt aactcatttt
     3121 caaaattttc aattgaacct ttgacatgat tcactactac aaagaaattg gatttttctt
     3181 taatattaat ttctgcatct gaatccatca tttcccgtgt tttatctgtc tttaattcaa
     3241 aatgtggtgg caatgtgtgt aaatctgatt caaagaataa tgctacattc aataaatcac
     3301 tgacaacggc actacctgtt gctaaactgc ctgctccttt tccataaaac atcgtgtcac
     3361 caacggcatc accaataaca taaatcgcgt tatattcatc ctctacagct gctaattgat
     3421 gctttttatc aattaacgtt ggttctaccg atgcattaac tttgccattt tcatatattc
     3481 ccttaccaat caatttaatt ttatacccca gttgatcggc tacattaata tcagttaaag
     3541 ttacgccact aattcctctt cgtttaacgt cgtttaattt aatgacttgg ttaaatgata
     3601 aatatgatgt aatgacaact ttacgcgctg catctacccc ttctacatca tcagttggat
     3661 ccgcttcagc aaaaccaagt cttttcgctt catcaagtgc ttcctcaaat gtcgtttgct
     3721 ctttagtcat tttagataaa ataaaattag aggtaccatt taaaattccc ataaattttg
     3781 aaatattatt cgcattcaaa ccattattta tggcatttac gatcggaata ccacctgcta
     3841 cactcgcttc aaactttaaa gctacaccat tttcttctgc taaatcttct aaaagtttaa
     3901 gatgtactgc taataaatct ttatttgcgg taataacatg ttttttattt ttaagtgctg
     3961 ttcttaacca atctacagtt ggttcaattc ctcccatgac ttcaacgata atatctaatg
     4021 aatcatcatt taaaatttca ttaacatctt cagttaaatg atattggcta atatttagcg
     4081 gtctcttttt agatttatct cgaacaagaa tatgctttat gacaatatct ttatttaatg
     4141 tatcttgaat ttgctgtcgg ttctcttcga tgattttaac aacaccagat ccgacagtac
     4201 ctaatcctaa taatgctata tttaattttt tcattttatg tacctccgtt tgtgcttttg
     4261 agaaaaacaa atgtattatt gaaaaacact agatgatagt atagtataga attattcttt
     4321 atttttgtaa agttcgtaat attcaaaata taaatacata atatcattct tacttacaaa
     4381 actgaataca attttctgat tttttagaaa ggttggcatg cactatgaaa gtttccaaat
     4441 tcggtggtag ctccgtctcc aatgctactc aaattaaaaa ggttttaaat attgttaatt
     4501 ctgatccaga gcgaaagatt gttatcgttt ctgctccagg taaaagacat gataatgata
     4561 ttaaaacaac tgatttgtta atcagattat atgaaaaggt cattaatcat cttgattatc
     4621 atgataaaaa aagagaaatt attcagcgtt atgatgatat tgtaaaagaa ttgcaaatgg
     4681 atgaaagtat tttacggacg atagatgtga ctttggaaca ttatattaat caattaaaaa
     4741 atgaaccaaa gagactatta gatgcattac tttcttgtgg tgaagatttt aatgcgcaat
     4801 tgatagcttt atataataat agtcaaggta taccaacaaa atatatatca ccgaaggaag
     4861 cgggtatttt tgttactgac gtcccacaag aagcacaaat attagatagt gcttactctg
     4921 aaattaaaaa attaagagat tatgatgaaa aactaattat cccaggattt tttggcgtat
     4981 ctcatgaagg atatatagtt acatttccac gtggcggatc agatataact ggcgccatca
     5041 tatcaagtgg tgttagagcc acaatttatg aaaatttcac cgatgtgtct ggtatttata
     5101 aagctaatcc aaatatcatc aaagatcctg aattaatcga agaaatcact tatcgagaaa
     5161 tgcgtgaatt atcttatgct ggttttagtg ttttccatga tgaagcctta caaccacttt
     5221 ataaagatcg catccctgtg gtgattaaaa atacgaaccg ccctcaagat aagggtacat
     5281 ttatagtgca tgatagagaa ataaatgcga aaaatgtgat tagcggtata agttgtgata
     5341 aagattttac cgttattaat atcaaaaagt atttaatgaa tagacaagtc ggctttacta
     5401 gaaagatatt aggtgtctta gaggataata atatttcatt tgatcatatg ccttcaggta
     5461 ttgatactat cagtatcgtt atgcgttcaa aacaaattca aaataaagaa acaaaagtat
     5521 taaatgaaat tcgtcaaaaa tgtgatgttg atgagctaag tattgagcat gatttagcaa
     5581 tattaatgat tgtcggcgaa ggtatgaata aagtcatcgg tactgcaaat aaaattacac
     5641 atgccttagc tgaatcaaaa attaacttaa aaatgattaa ccaaggtgct tcggaaatct
     5701 cgatgatgtt tggaattcat gtagatgatg ctgaaaaagc tgtattatct acatatgagt
     5761 tttgctatca cggggttgca ttaaaaaact tgtgtgataa aaactaatat aaaagcaatt
     5821 cgtaacacat gtattcaact gcgttatgaa ttgctttttc ttaattattt attgttctct
     5881 ggattgcatc cttaatacgt tgctcaccta ctacaaattt taaaataaaa aattgatagt
     5941 agcttaatgc atctatgact cgtttataat tggaataatg atgatactga tggtgttgca
     6001 ttagatcata cattagaacg atctccggtt taatataatc cacatcccaa tttaaactat
     6061 ggaagtatat gttactctta ggtatacgta tattcggatc caatctaaaa atccattcat
     6121 cgtttactac atcataaacg aaaatactca ttattgttgt atcatcatat tgcacataaa
     6181 cttttgcaat ttttgaaagt gataaatctt ctttagctat tgcttgatct tgcttatcta
     6241 tgtatagtaa cgcatatgat tgcggaataa ctttcacaat atcacttaaa cgcttacgtt
     6301 ccacgcatat ttcaacaata gaaggtaatt ttaattcatc atttaagaaa agctgcatgg
     6361 caacttcacc gtgaaattta aataattgcg atgatgtttt cattaaattg tccactaatt
     6421 gttctttagc attcatttca ttcactctca aatacacctc ctaagcaagc gctttcacaa
     6481 atatcattat acattttaaa attaatgaat tcaatcttca aaatactatg agtgacctaa
     6541 ttctgtcatt taaatgaccg ttataacata aattcgacat aaacttgaat tcacgaatct
     6601 ttcacgtcat attcattgaa catcaatatc tgtcagttta taatttgatt ataacaatga
     6661 tgaactaaaa tcaactaata aaaatgtggg aaattgaata acaattaatc tttattaatt
     6721 attattgtat acaaagttaa agaagtcaaa aaggattggt gaaaaaatgt ctttttatgt
     6781 tgtacttatc attatcattg tagcattaat tggtatatta gttttaaatc aaaggtatag
     6841 taatagtaaa atagataccg aagtttatgc aagaaagcaa cttattaaaa agaacaaagc
     6901 attaagtgca gaaaatgctg aattaagaag tcaaatgctg agttctaata atgatgttgg
     6961 ccaccatgca tacaaaaatg ctaaaagaga attacgcaag attttagata gctatctcga
     7021 aaatggtaaa ttaaaatatt acgatattat cgttacaagc aatttagcta ctaaacatcc
     7081 gtttttcgag tatgcacgtt catttgattt tatcattgtt tcagatatcg gtttaataaa
     7141 cgttgatgta aaaagctggg gtgaaaaaac attttatcac tttgatgtac ctgatgagca
     7201 tgatacagaa ataagcaata gtaatattga aaaagttgtc ggtcattaca ttagccagca
     7261 atatcatgat cagtttaatt catcaagaaa gtctatctat acttttacag aaacagttca
     7321 accaaatcgt gttatttatg atttttatga ttatgatccg tatcaattag cagcaaataa
     7381 tgcgaaagca ttaaaggatc atattgaaca aaactttaac tttaaagtcc aaagtaccgg
     7441 tgttatttac tttagtgatg gtacagttaa tatcattcaa ggttctgaag aaagagataa
     7501 atatgttgat acagtttcaa caaaatcttc gttacgacgt attattagcg aagccataga
     7561 gctatctaaa caccctttaa ataaagaaca ggttgatcaa atcacagcta tattcaagta
     7621 aaatcaaagg cataataatc cattacgctc gatacgcact atggttatta tgcctttaaa
     7681 ttatttctat atataaattt atggtaataa taaatcgatg cttcaagtat attttacttt
     7741 aatatatata atcacctatt taattattta ctccaaatat ttaatttctg ttgtttagct
     7801 ttattttgtg cttctataaa tacatttcta tatttgccat ttggtgaaaa atacttctct
     7861 ctagcaagtc ccttttccac taattcctta ttgtacatac gatctttact tatccataca
     7921 tacgccaatg ttctaccata gcgatcttgt ttttctttat catattctaa ataaacatct
     7981 tgatttgtta atgtcttctt actataattt gatgcttctt tgccaaatgg ttgtacaggc
     8041 gtattcggtt tcaccgtttc tggcgtatca acccctataa gcctaacttt aatttcttta
     8101 ccattttgat ttgcaataaa tgtatcacca tccacaactc tttgaacatg tactttatct
     8161 ttaccattta aatcttgtac agtttcatga ttcgtcccct ttttgaaagg acccgtatga
     8221 ttcataaatt gaaatgctaa tacacctaca ataatgatgg ctaccacaat catagcaagc
     8281 gatttatttg acttcatatt gccacctact tctctttttt cattccgatt aatcatataa
     8341 aagttaagaa atatcgtcaa tagccatatc tacatatttc aatattttgg aaagctatag
     8401 catgatataa tgttattgtt aaggagtgaa aataaatgaa catttcaaat gaaaatacag
     8461 tcacattaat tgcagtaatt atcgcaatta tcattggtat tttcctacaa atatttttca
     8521 aattaccatt aatagtgacg gcagtactat ctattttatt gggtatcttt gtaggcttta
     8581 tcgtttactt gattgtttcg ttttataata agcgaaaaaa ttagttaaca aatctaaatt
     8641 gcgagtacca aaataaaagt attgagtaag attttgatta ttgactcact ttccaagtct
     8701 cttaaatcaa aacactcaat acttttttgt ttgtatttag atccagcctt tttcatttgc
     8761 ctttttccaa gcatcaaaac gattatctgc aaataattta tcaattataa cagatgtata
     8821 atttctaact gttccatctg tcaaaaataa tttttcactt atttctttac tacttaaacc
     8881 attgccaatt tcccttaata caatttgttc tttgggcgtt aatgggtttt tatctacaaa
     8941 aaatgaagtc atcaatgtgg cgctatattc tttctctccg ttatttactt tattaatggt
     9001 ttccaccaat tcttctatag aacgttcttt taaaacatat gcatccacat cattcacaac
     9061 tgctttttca aagtatcccg gtcttttaaa agttgttaca ataatcactt taatattcaa
     9121 atgctttttt ctaatttccg ctaaaacttc aagtccagtc atgcctggca tttctatatc
     9181 taaaaaaaca acgttaggat tatattcttc aataagtttc attgcatcga gaccattatc
     9241 agtatctgct aaaatttcaa aatcaccatg tagtttaatt aattgaacca ttgcctgtcg
     9301 taacatattt tgatcttctg caataattaa agatgtcatg ctattcctcc tgttgagatt
     9361 tcaatgatta ttttagttcc atttgttgag tcgactgcta aagttccatt taaatgttgt
     9421 acacgctgtg aaatactttt cacctcacaa tcactatcga tacctttgcc atcatcttca
     9481 atcataagca gtaatttatg attgtttaca gtttttaatt taccatgaac ttttgaagca
     9541 tttgcatgtt taataacatt atttatcgct tcacgcgtaa tcataactaa catagattgt
     9601 ttagtaggac ttaatacttg cgctaattct ttattttcaa atgtaaaatc aatatcagca
     9661 tcttttaaaa ctttacgtat actatcaatc tcttcaataa atgatggtaa ttttacatca
     9721 tcaataattt ctcgaacttt gttcaatgat tcacgagata atttattaat tgctaataat
     9781 tcagctttta ctttttctac atcagcatct attagtttat aagctaattc tgattttaat
     9841 gataaacttg caaacacatg ccctaacgta tcatgtaagt cttgaccaat tctatgtcgc
     9901 tcttgttcag caattaat
//
LOCUS       DAKTGS010000109         9763 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.109, whole genome shotgun sequence.
ACCESSION   DAKTGS010000109 DAKTGS010000000
VERSION     DAKTGS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.109"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            393..1289
                     /gene="xerC"
                     /locus_tag="O0286_002122"
     CDS             393..1289
                     /gene="xerC"
                     /locus_tag="O0286_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCY0170486.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1286..1831
                     /gene="hslV"
                     /locus_tag="O0286_002123"
     CDS             1286..1831
                     /gene="hslV"
                     /locus_tag="O0286_002123"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCY0170487.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1897..3300
                     /gene="hslU"
                     /locus_tag="O0286_002124"
     CDS             1897..3300
                     /gene="hslU"
                     /locus_tag="O0286_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCY0170488.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3325..4098
                     /gene="codY"
                     /locus_tag="O0286_002125"
     CDS             3325..4098
                     /gene="codY"
                     /locus_tag="O0286_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCY0170489.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            4149..4241
                     /locus_tag="O0286_002126"
     CDS             4149..4241
                     /locus_tag="O0286_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170490.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            4440..5207
                     /gene="rpsB"
                     /locus_tag="O0286_002127"
     CDS             4440..5207
                     /gene="rpsB"
                     /locus_tag="O0286_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCY0170491.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            5241..5354
                     /locus_tag="O0286_002128"
     CDS             5241..5354
                     /locus_tag="O0286_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170492.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5389..6270
                     /locus_tag="O0286_002129"
     CDS             5389..6270
                     /locus_tag="O0286_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCY0170493.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6407..7129
                     /locus_tag="O0286_002130"
     CDS             6407..7129
                     /locus_tag="O0286_002130"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCY0170494.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            7148..7702
                     /gene="frr"
                     /locus_tag="O0286_002131"
     CDS             7148..7702
                     /gene="frr"
                     /locus_tag="O0286_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCY0170495.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            8075..8845
                     /locus_tag="O0286_002132"
     CDS             8075..8845
                     /locus_tag="O0286_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCY0170496.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            8852..9634
                     /locus_tag="O0286_002133"
     CDS             8852..9634
                     /locus_tag="O0286_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCY0170497.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT         3638 a         1331 c         1893 g         2901 t
ORIGIN      
        1 tattcattct taagtcatca acgagtaagt aatgactttc taaatggaaa atacttatcc
       61 tagtcttttt aattttggaa ttgttacgta tttctgacaa tttagaattc gcattcaaaa
      121 aatatctaaa taaataacac gcaataagtt gattgatgta acatgtaaga gaatgtttta
      181 aataaacttt atttaaaagg caatgaaata ataaatggca aggctattaa taaagacttt
      241 tagtaattaa tttaaaaaag aggtattcta attaacaggt tttccgatta gttacaatta
      301 tttaattctc aaaagattta gaattgatta tcaaattact gtaagccctt tgctgtatat
      361 gctacaattc ttattgatgg agggtaaatg tattgaatca tattcaagat gcgtttttaa
      421 atacattgaa agttgaacgg aatttttcgg aacacacatt gaaatcatat caagatgact
      481 taattcagtt taatcaattt ttagaacaag aacatttaga gttgaatact tttgaataca
      541 gagatgctag aaattatttg agctatttat attcaaatca tttgaaaaga acatctgttt
      601 ctcgtaaaat ctcaacgtta agaactttct atgaatattg gatgacgctt gatgagaaca
      661 ttattaatcc atttgttcaa ttagtacatc cgaaaaaaga aaaatatctt ccgcaattct
      721 tttacgaaga agaaatggaa gcgttattca aaactgtaga agaggacact tcaaaaaatt
      781 tacgggatcg agttattctt gaattgttgt atgctacagg catccgtgtt tcggaattag
      841 taaatattaa aaaacaagat atagattttt acgcgaatgg tgttaccgta ttaggaaaag
      901 ggagcaaaga gcgctttgta ccgtttggtg cttattgtag acaaagcatc gaaaattatt
      961 tagaacattt caaaccaatt cagtcatgca atcatgattt tcttattgta aatatgaagg
     1021 gtgaagcaat cactgaacgc ggtgtacgat atgttttaaa tgatattgtt aaacgaacag
     1081 caggcgtaag tgagattcat ccccacaagc tcagacatac atttgcaacg catttattga
     1141 atcaaggtgc agacctaaga acagtacaat cgttattagg tcatgttaat ttgtcaacaa
     1201 ctggtaaata tacacacgta tctaaccaac aattaagaaa agtgtatcta aatgcacatc
     1261 ctcgagcgaa aaaggagaat gaaacatgag taatacaaca ttacatgcaa caacaattta
     1321 tgctgtaaga cataatggga aagcagctat ggctggagat gggcaagtaa cgcttggtca
     1381 acaagtcatc atgaaacaaa cggcaagaaa agtgcgacgt ttatatgaag gtaaagtgtt
     1441 agctggtttc gcaggtagtg tagcagatgc gtttacgtta tttgaaaaat tcgaaacaaa
     1501 attacaacag tttagtggta acttagaaag agctgctgtt gaattggcac aagaatggcg
     1561 aggcgataaa caattacgtc aattagaagc tatgctaatt gtaatggata aagatgctat
     1621 tttagttgtc agtggaactg gcgaagttat tgctccagat gatgacctta tcgctattgg
     1681 atcaggaggc aactacgcat taagcgcagg acgtgcattg aaacgccatg catcgcattt
     1741 gtctgctgaa gaaatggcat atgagagctt gaaagtagcg gctgatattt gtgtctttac
     1801 caacgataat attgttgtcg aaacactata ataatcagag cacgataaat aattacgagc
     1861 aattaatttt agttaaaaga cggaggaatg aaattaatgg atacagctgg aataagatta
     1921 actccaaaag aaatcgtatc taaattaaat gaatacatcg ttggacaaaa tgatgctaaa
     1981 cgtaaagtgg caattgccct acgtaatcga tacagaagaa gtttattaga tgaggaatca
     2041 aagcaagaaa tttcacctaa aaatattttg atgattggac caaccggcgt tggtaaaact
     2101 gaaattgcaa gaagaatggc caaagttgtc ggcgcgccat ttataaaagt agaagctact
     2161 aaatttactg aggtaggtta tgtaggacga gatgttgaaa gtatggttag agatcttgtt
     2221 gatgtttcag taagattagt caaggcgcag aaaaaatcat tggtacaaga tgaagcaaca
     2281 gctaaggcca atgaaaaact tgttaagtta ttagttccaa gtatgaaaaa gaaagcgtct
     2341 caaacgaata atcctttaga gtcacttttc ggaggtgcaa ttccaaattt cggacaaaat
     2401 aacgaagatg aagaagaacc acctactgag gaaattaaaa caaaacgttc tgaaattaag
     2461 agacagctag aagaaggcaa acttgaaaaa gaaaaggtaa gaattaaagt cgaacaagat
     2521 cctggtgctt taggtatgct aggtacaaat caaaatcagc aaatgcaaga gatgatgaat
     2581 caattaatgc ctaaaaagaa agttgagcga gaagttgctg ttgagacggc aaggaaaatc
     2641 ttagctgata gttatgcgga tgaactaatt gatcaagaaa gcgctaacca agaagcgctt
     2701 gaattagcag aacaaatggg tatcatcttt atagatgaaa tcgacaaagt tgcgacgaat
     2761 aatcataata gtggtcaaga tgtctcaaga caaggtgttc aaagagatat tttacctata
     2821 cttgaaggta gcgttattca aaccaaatat ggtactgtga atactgaaca tatgctgttt
     2881 ataggtgctg gagctttcca tgtatctaag ccgagtgact tgataccaga attgcaaggt
     2941 cgttttccga ttagagttga acttgatagt ttatcggtag aagattttgt aagaattttg
     3001 acagaaccaa aattgtcatt aattaaacaa tatgaagcat tgcttcaaac agaagaagtt
     3061 actgtaaact ttaccgatga agcaattact cgcttagctg agattgctta tcaagtaaat
     3121 caagatacag acaacattgg tgcacgtcga cttcatacaa ttttagaaaa gatgctagaa
     3181 gatttatcat tcgaagcacc aagtatgccg aatgcagttg tagatattac cccacaatat
     3241 gttgatgata aattaaaatc aatttcaaca aataaagatt taagtgcatt tattctataa
     3301 aaatatacaa aaggagaaaa attcatgagc ttattatcta aaacgagaga gttaaacacg
     3361 ttacttcaaa aacacaaagg tattgcggtt gattttaaag atgtagcaca aacgattagt
     3421 agcgtaactg taacaaatgt atttattgta tcgcgtcgag gtaaaatttt aggatcgagt
     3481 ctaaatgaat tattaaaaag tcaaagaatt attcaaatgt tggaagaaag acatattcca
     3541 agtgaatata cagaacgatt aatggaagtt aaacaaacag aatcaaatat tgatatcgac
     3601 aatgtattaa cagtattccc acctgaaaac agagaattat tcatagatag tcgtacaact
     3661 atcttcccaa ttttaggtgg aggggaaaga ttaggtacat tagtacttgg tcgagtacat
     3721 gatgatttta atgaaaatga tttggtacta ggtgaatatg ctgctacagt tattggtatg
     3781 gaaatcttac gtgagaagca tagtgaagta gaaaaagaag cgcgcgataa agctgctatt
     3841 acaatggcaa ttaattcatt atcttattct gaaaaagaag cgattgaaca tatctttgaa
     3901 gaacttggcg gtacggaagg cctattaatc gcatcaaaag ttgcagatag agttggtatt
     3961 actagatctg taattgtaaa tgcactacgt aaattagaaa gtgctggtgt aattgaatca
     4021 cgttctttag gaatgaaagg tactttcatt aaagttaaaa aagaaaaatt cttagatgaa
     4081 ttagaaaaaa gtaaataagt cgatgagtct gggacataat tcaatgtcct agtcgtgacg
     4141 atgttatctt ggcggtagtc aatgaaatga ctttgatgat tatgttgaag tcatttcttt
     4201 atttttgtct aattttcaat caaattgata tttcacatta acttgataga attaccttta
     4261 aatttatgga aaaatgcttg aaaaacattt gctttagaca cggcattatg ttatatttat
     4321 tttcggctat aaagccaata cacacatatc aaacgtgatt gtgtaaaggg tgcaatattt
     4381 atatattgtc tttacaatag tttgctatgg aggtaattaa ccaataggag gaatttataa
     4441 tggcagtaat ttcaatgaaa caattactag aagcgggtgt tcacttcggt caccaaacac
     4501 gtcgttggaa cccaaaaatg aaaaaatata tcttcactga gagaaatggt atttatatca
     4561 tcgacttaca aaaaacagtg aaaaaagtag acgaggcata caacttcttg aaacaagttt
     4621 cagaagatgg tggacaagtc ttattcgtag gaactaaaaa acaagcacaa gaatcagtta
     4681 aatctgaagc agaacgtgct ggtcaattct acattaacca aagatggtta ggtggattat
     4741 taactaacta taaaacgatc tcaaaacgaa tcaaacgtat ttctgaaatt gaaaaaatgg
     4801 aagaagatgg tttattcgaa gtattaccta aaaaagaagt agtagaactt aaaaaagaat
     4861 acgaccgttt aatcaaattc ttaggcggaa ttcgtgatat gaaatcaatg cctcaagcat
     4921 tattcgtagt tgacccacgt aaagagcgta atgcaattgc tgaagctcgt aaattaaata
     4981 ttcctatcgt aggtatcgtt gacactaact gtgatcctga cgaaattgac tacgttatcc
     5041 cagcaaacga cgatgctatc cgtgcggtta aattattaac tgctaaaatg gcagatgcaa
     5101 tcttagaagg tcaacaaggc gtttctaatg aagaagtagc tgcagaacaa aacatcgatt
     5161 tagatgaaaa agaaaaatca gaagaaacag aagcaactga agaataatca actgttgaat
     5221 ctgacttaga tatagtttaa atgggtgata agatattaat gcttatcacc ttttttaaaa
     5281 agaaaatcga ggcaaattac aaatattcaa ttagagtatt ggcaatcttg cctataataa
     5341 tgctaaaatc ataatatata aaatgataac ttattggagg aataatgaat ggcaactatt
     5401 tcagcaaaac ttgttaaaga attacgtgaa aaaactggcg cgggtatgat ggattgtaaa
     5461 aaagcgctaa ctgaaactga tggtgacatc gataaagcga ttgactacct acgtgaaaaa
     5521 ggtattgcta aagcagctaa aaaagcagac cgtattgcgg ctgaaggttt agtacatgta
     5581 gaaactaaag gtaacgacgc agttatcgtt gaaatcaact ctgaaacaga ctttgttgct
     5641 cgtaacgaag gtttccaaga gttagttaaa gaaatcgcta atcaagtatt agatacaaaa
     5701 gctgaaactg ttgaagcttt aatggaaaca actttaccaa atggtaaatc agttgatgaa
     5761 agaattaaag aagcaatttc aacaatcggt gaaaaattaa gtgttcgtcg ttttgctatc
     5821 agaactaaaa ctgataacga tgctttcggc gcttacttac acatgggtgg acgcattggt
     5881 gtattaacag ttgttgaagg ttcaactgac gaagaagcag caagagacgt tgctatgcat
     5941 atcgctgcaa tcaaccctaa atatgtttct tctgaacaag ttagcgaaga agaaatcaac
     6001 cacgaaagag aagttttaaa acaacaagca ttaaatgaag gtaaaccaga aaacatcgtt
     6061 gaaaaaatgg tggaaggacg tttacgtaaa tacttacaag aaatttgtgc tgtagatcaa
     6121 gacttcgtta aaaaccctga tgtaacagtt gaagctttct taaaaacaaa aggtggaaaa
     6181 cttgttgact tcgtacgcta tgaagtaggc gaaggtatgg aaaaacgcga agaaaacttt
     6241 gcggatgaag ttaaaggaca aatgaaataa tctgtcataa agtaaaacaa ggaagaagac
     6301 acctttaatg ttgctttatt aaaatgtaaa tcattctaat aaaacgacaa ctgtgtcttc
     6361 tttacttgta tatgttacat atattcacga tagagaggat aagaaaatgg ctcaaatttc
     6421 taaatataaa cgtgtagttt tgaaactaag tggtgaagcg ttagctggag aaaaaggatt
     6481 tggcataaat ccagtaatta ttaaaagtgt tgctgagcaa gtggctgaag ttgctaaaat
     6541 ggactgtgaa atcgcagtaa tcgttggtgg cggaaacatt tggagaggta aaacaggtag
     6601 tgacttaggt atggaccgtg gaactgctga ttacatgggt atgcttgcaa ctgtaatgaa
     6661 tgccttagca ttacaagata gtttagaaca attggattgt gatacacgag tattaacatc
     6721 tattgaaatg aagcaagtgg ctgaacctta tattcgtcgt cgtgcaatta gacacttaga
     6781 aaagaaacgc gtagttattt ttgctgcagg tattggaaac ccatacttct ctacagatac
     6841 tacagcggca ttacgtgctg cagaagttga agcagatgtt attttaatgg gcaaaaataa
     6901 tgtagatggt gtatattctg cagatcctaa agtaaacaaa gatgcggtaa aatatgaaca
     6961 tttaacgcat attcaaatgc ttcaagaagg tttacaagta atggattcaa cagcatcctc
     7021 attctgtatg gataataaca ttccgttaac tgttttctct attatggaag aaggaaatat
     7081 taaacgtgct gttatgggtg aaaagatagg tacgttaatt acaaaataaa tttagaggtg
     7141 taaaataatg agtgacatta ttaatgaaac taaatcaaga atgcaaaaat caatcgaaag
     7201 cttatcacgt gaattagcta acatcagtgc aggaagagct aattcaaatt tattaaacgg
     7261 cgtaacagtt gattactatg gtgcaccaac acctgtacaa caattagcaa gcatcaatgt
     7321 tccagaagca cgtttacttg ttatttctcc atacgacaaa acttctgtag ctgacatcga
     7381 aaaagcgata atagcagcta acttaggtgt taacccaaca agtgatggtg aagtgatacg
     7441 tattgctgta cctgccttaa cagaagaacg tagaaaagag cgcgttaaag atgttaagaa
     7501 aattggtgaa gaagctaaag tatctgttcg aaatattcgt cgtgatatga atgatcagtt
     7561 gaaaaaagat gaaaaaaatg gcgacattac tgaagatgag ttgagaagtg gtactgaaga
     7621 tgttcagaaa gcaacagaca attcaataaa agaaattgat caaatgattg ctgataaaga
     7681 aaaagatatt atgtcagtat aaaactaata tacaatgaca tattaaaatg ccagtattaa
     7741 acgataatgt aacatttaaa atgggcatgt ttaattaaat caaagatgca tgtgataatt
     7801 taaattcaca atgagcataa aaatggtgtt taaacaagtt aattaaacat atactttata
     7861 aataataggc attaggtata ttgctataat aaagttatgt aatttttaac ctcagtatgt
     7921 atgtcacatt tctggtgtaa actgtaccga gtcagacttt ggtacagttt ttttatttgc
     7981 ttattcaatg cattaaatga gtatgataaa atgataatga ttgtttagta acttatacta
     8041 tatgacagag atgatcaggc tcggaggaaa gaccatgttt aaaaagctaa taaataaaaa
     8101 gaacactata aataattata atgaagaatt agactcgtct aatatacctg aacatatcgc
     8161 tattattatg gatggtaatg ggcgatgggc taagaagcga aaaatgccta gaattaaagg
     8221 tcattacgaa ggtatgcaaa caataaaaaa aattactagg gtagctagtg atattggtgt
     8281 taagtactta actttatacg ccttttccac tgaaaattgg tcaagacctg aaagtgaagt
     8341 aaattatatt atgaatttgc ctgtcaattt cttaaagaca ttcttaccgg aactaattga
     8401 aaaaaatgtc aaagttgaaa caattggatt tactgataag ttgccaaaat caacgataga
     8461 agcaattaat aatgctaaag aaaagacagc taataatacc ggcttaaaat taatatttgc
     8521 aattaattat ggtggcagag cagaacttgt tcatagtatt aaaaatatgt ttgacgagct
     8581 tcatcaacaa ggtttaaata gtgatatcat agatgaaaca tatataaaca atcatttaat
     8641 gacaaaagac tatcctgatc cagagttgtt aattcgtact tcaggagaac aaagaataag
     8701 taatttcttg atttggcaag tttcgtatag tgaatttatc tttaatcaaa aattatggcc
     8761 tgactttgac gaagatgaat taattaaatg tataaaaatt tatcagtcac gtcaaagacg
     8821 ctttggcgga ttgagtgagg agtagtatag tatgaaagtt agaacgctga cagctattat
     8881 tgccttaatc gtattcttgc ctatcttgtt aaaaggcggc cttgtgttaa tgatatttgc
     8941 taatatatta gcattgattg cattaaaaga attgttgaat atgaatatga ttaaatttgt
     9001 ttcagttcct ggtttaatta gtgcagttgg tcttatcatc attatgttgc cacaacatgc
     9061 agggccatgg gtacaagtaa ttcaattaaa aagtttaatt gcaatgagct ttattgtatt
     9121 aagttatact gtcttatcta aaaacagatt tagttttatg gatgctgcat tttgcttaat
     9181 gtctgtggct tatgtaggca ttggttttat gttcttttat gaaacgagat cagaaggatt
     9241 acattacata ttatatgcct ttttaattgt ttggcttaca gatacagggg cttacttgtt
     9301 tggtaaaatg atgggtaaac ataagctttg gccagtaata agtccgaata aaacaatcga
     9361 aggattcata ggtggcttgt tctgtagttt gatagtacca cttgcaatgt tatattttgt
     9421 agatttcaat atgaatgtat ggatattact tggagtgaca ttgattttaa gtttatttgg
     9481 tcaattaggt gatttagtgg aatcaggatt taagcgtcat ttcggcgtta aagactcagg
     9541 tcgaatacta cctggacacg gtggtatttt agaccgattt gacagcttta tgtttgtgtt
     9601 accattatta aatattttat taatacaatc ttaatgctga gaacaaatca ataaacgtaa
     9661 agaggagttg ctgagataat ttaatgaatc tcagaactcc ttttgaaaat tatacgcaat
     9721 attaactttg aaaattatac gcaatattaa ctttgaaaat tat
//
LOCUS       DAKTGS010000110         9602 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.110, whole genome shotgun sequence.
ACCESSION   DAKTGS010000110 DAKTGS010000000
VERSION     DAKTGS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.110"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(242..721)
                     /gene="rlmH"
                     /locus_tag="O0286_002134"
     CDS             complement(242..721)
                     /gene="rlmH"
                     /locus_tag="O0286_002134"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCY0170498.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1089..3407)
                     /gene="adsA"
                     /locus_tag="O0286_002135"
     CDS             complement(1089..3407)
                     /gene="adsA"
                     /locus_tag="O0286_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCY0170499.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(3635..4435)
                     /locus_tag="O0286_002136"
     CDS             complement(3635..4435)
                     /locus_tag="O0286_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0170500.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(4825..5613)
                     /locus_tag="O0286_002137"
     CDS             complement(4825..5613)
                     /locus_tag="O0286_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCY0170501.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(5614..6948)
                     /locus_tag="O0286_002138"
     CDS             complement(5614..6948)
                     /locus_tag="O0286_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170502.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(6941..8767)
                     /gene="walK"
                     /locus_tag="O0286_002139"
     CDS             complement(6941..8767)
                     /gene="walK"
                     /locus_tag="O0286_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCY0170503.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(8780..9481)
                     /locus_tag="O0286_002140"
     CDS             complement(8780..9481)
                     /locus_tag="O0286_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0170504.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         2673 a         1925 c         1480 g         3524 t
ORIGIN      
        1 tatatttagt tgcagaaaga attttctcaa agctagaact ttgcttcact ataagtattc
       61 agtataaaga atatttcgct attatttact tgaaatgaaa gactgcggag gctaactatg
      121 tcaaaaatca tgaacctcat tacttatgat aagcttctta aaaacataac agcaattcac
      181 ataaacctca tatgttctga tacattcaaa atccctttat gaagcggctg aaaaaaccgc
      241 atcatttatg atatgcttct cctcgcataa tcttaaatgc tctgtacact tgttcaatta
      301 acacaacccg catcatttga tgtgggaatg tcattttgct gaatgatagt gcgtagttac
      361 tgcgttgtaa gacgtccttg tgcaggccgt ttgatccgcc aatgacgaaa acaaagtcgc
      421 tttgcccttg ggtcatgcgt tggttcaatt cttgggccaa tccttcggaa gatagcatct
      481 ttccttgtat ttctaatgta atgactgtgg attgtggttt gattttggct agtattcgtt
      541 ggccttcttt ttcttttact tgctcaattt ctttgtcact catattttct ggtgcttttt
      601 cgtctggaac ttctatgatg tctatcttgg tgtatgggcc taaacgtttt tcatattctg
      661 ctatggcttg cttccaatat ttctctttta gtttccctac agctaaaatg gtgattttca
      721 tgtcgtttgg tcctccaaat tgttatcaac tttccagtta tccacaagtt attaacttgt
      781 tcacactgtt ccctcttatt ataccaatat tttttgcagt ttttgatatt ttcctgacat
      841 ttatgcccga tttttagtgt ataacctgtg tatttgtgga taactctgtg ggcattgtgg
      901 gtatttctct ttaaattgta cacaattctg gataactatc cccatttctg tggataaaca
      961 ccttgatgtc ttattactta tcctcgtttt tatatcccat atttatcaac ggttatcgct
     1021 ttttttcata aaaaaacact accgtacctc ttatttaaga aatacagtag tattattttc
     1081 aatatatttt agctagcttt tctacgtcgt acagtcgcga ttaaacctat acctgctaat
     1141 aatacaaaca tcgcttctgg gcttgaactt tgattagttc cagtttttgg taactgtttc
     1201 tcatgcgcgc tacctttagg cactgacatt ctagccaatt gtttcccact cttgcttgat
     1261 acagtagctc cttctattgt gcgaccagaa ccttctgtac cgctactaac agttcctcta
     1321 tgcgctagca acaatacaac tttacctgga gctggttttt tcgctggatc catcactttg
     1381 tcgtcaccaa ttggttgtgt atctttacca gacttactac ctttgctacc ttgttgtcct
     1441 ttagctggtt gttcacttac tgctggttta cctaataaca tacgttgtgg ttctgtcgta
     1501 tcatacttag ctaagttagc tgtttttaaa taacttgcta gtacttgatc taatgaaata
     1561 ccttcttctc taggaccacc gaacatacta tatccgtcgc cacctgatgc tgtgaagtca
     1621 ttcatcgtta cgtgatatac acgttttaaa tcaatatttt caaacttacc tgtctcttta
     1681 tttaaaattt gaatagcatt aattcgtttg ccagacggtt tatttatatc atagtaaaca
     1741 cggattgaat cagagatatg tagtaaaccg ccattcgctg ttaacactgt cttaccgtcc
     1801 ttttgtgttg ttggtgcgcc taaactatgt tcgaaagccg tccagacgtc tgaacctttt
     1861 acatcaattt gcgcaatcgt atttccaaat ggtaatactg agattaaatc atagcgtgtc
     1921 accttacctt ttgcgataga ggcacgaata cctccaccat ttgtcacggc aaagtcagtc
     1981 tttttagaga aattcttaac gccatacgct tccatagcat ctgcaatcgc gtttcctaaa
     2041 tttgtttcac gcgttctaac gtcatctctt tctcctttga aatcaatggt attgtttgga
     2101 ataattacct ctgcagtttg tgctctaaat gtttgatcag cttgattaat ttgttcagct
     2161 aatgctttgt tcggtgttac attttcaacg tctttaacat taatcaatga cggtttaata
     2221 ttcgatacct ctccattgcg ataattaaat gtaatcttac cgatattcgc aagtgctgta
     2281 cctgtttgtg ccaatgcatc attgttataa atttgaccat tttgaagtac tgtatgtgaa
     2341 tgaccatcaa taactgtaat acgtttcttc aattgtggat tttgacttaa ttgtttcact
     2401 aagtaatcac cacgccatgt ttcttgtgtt gaaggatcaa ttcctaaatg tgatataaca
     2461 acaaatgtat ctacgtcttt ataaatacgc atcatttccg ctgtcacact ttgtaatgga
     2521 tctctaaatt caacgccttt aatgccttca ggtcttgttt tcgtctttgt ttctggtgtc
     2581 gttacaccaa taattccata acgaatacca ttttttgtta caatcgttga aggcttaaac
     2641 gcgcgttttc catctttata aacgttagta cttagcatcg ggaagtctaa cataccctct
     2701 aactttttca actgatcgta tccaaagtca aattcatggt taccgactgc catagcatca
     2761 taacctactg cattcattgc tttagccatt tcttcacctt tagactggtt tgaaagtggt
     2821 aaaccttgga aggcgtctcc tgcgtctaac attaaatcag gcttttcttg ttcttttact
     2881 gtttttaatt tagccatacc gatgacacgc cctttttctt cggctagtcg gccatggata
     2941 tcatttgtat gtaatatttt atgtgttgtt gtttgtggtg cattagcagc aaacattcgt
     3001 ggtgaaagtg atgctgtttt agcatttgat aatttgaacg ttgctgtgtg agttggtttt
     3061 tgtgttgagg cttgttgtgt atctacgtcg cgtgtttcgt ttacttttgt tgaaactact
     3121 gtagatggtt tgttagatgg tgcgttggat gttactttgc tgtttgtatc atcagctgtt
     3181 ccctgcgctg atgctgatac gttggttgcc gcttctttgc cagaatgatg agccgcttcc
     3241 tttgttggcg gtacttgttg cttatctgtt gttgctttgt ctatcgttgt tactgcatgt
     3301 gctttcattg gtgtatgctg ctcagccgcg ttcgagactt gccataatcc cattacacta
     3361 aaaagcaaaa cgagccatac acttgtttta agtaataaag ctttcatttc gttccctcca
     3421 ttaactgtca tacaccaata tattatcata atccttatat taaaatgatt gattttatgt
     3481 aaattattta taaattacat ttaatatcga attattttac acatttcaat caagccgatt
     3541 caactcaaca acagccattt cccaccaaca acataaaaaa gcacccgata aagtcgtctc
     3601 acagcaatgc ggaactttat cggatgactc tcatttatat tgtatatatt ggcgttggaa
     3661 tagctttatc cgtatcacat agcaatactt ctttttccgt atcaatatcg tgttcgttca
     3721 atacttggcc aacactcata cgcgccaaat ctttcatgtt attgtcttgt gataaatgcg
     3781 ataggtaaat acgtttcgtg ttacctgtaa tcacgtctgt cattgcatga gccgcatcct
     3841 cattagatac atgacccata tcgcctaaaa tacgttgttt cgtcttccat ggataacgac
     3901 acattctcaa catatcgacg tcatgattac tctcaaaaat aaacgcatcg ctgccacgta
     3961 tcataccttt catacgatca gacacgtaac ccgtatccgt taaaatcgta aacttcttat
     4021 agttattatg gaaaatataa aattgcggat ctattgcatc atgtgacacg ttaaacgatt
     4081 caacatcgaa acctgcaata gattttgttt cataaggatt aaaaatgaat ttctgatcca
     4141 tagggatgcg actatctttc ttttcaattg cctgccaagt cttttcattc gcataaattg
     4201 gcaattgata tttacgcgcc aaaacaccta atcctttaat atgatcaata tgttcatggg
     4261 ttactaaaat accatttaaa tcttgaatat tacggtcaat ttgactaaac aattcttcca
     4321 ttttctttcc agtcaaacca acatcaacta atagactacc tttttcattt tctacaaaag
     4381 tggcgttacc tgtactacca cttgctaata cactcatgcg tatcaagcgg ctcatccttt
     4441 ctctttaaac agtcaatctc tctatatgac tgcaatatac atcagtgcaa atatacgatt
     4501 ttccctacag aaactatcag tatattagct gttttgtata cctttcctat tatgtaactt
     4561 tagttcatcg accggtttct tatatttaaa ttttgataat cacgtgcaat gaatttctta
     4621 attattataa caatgattag acaaatacta aagtttttaa aataacagtt acactaattt
     4681 tacaatgcgg ttctacttaa aatatgaata aatgcgcacg ttggagacgc aaaaacatca
     4741 gccataataa accgtaccag caaaaaagct agcaccgctt ctcacatatg atgagcttgc
     4801 aatgctagct tattacgatt catattaatg attaataatt ttagggttat tatttgtcgc
     4861 ttcgacatag taagtattcg ttttatcctt accgtcatgc ttcactttaa tttcccagtt
     4921 tggttgtaac aattgaacat tcgtttcatt cacgactgag tagtaaccga gtctagcatt
     4981 aatgacttca tcaccttttt tcaagtaacg attataatat aatgcctcga ttgcttttct
     5041 cacaccaatc acttgatgct tcttatctgc accatctgtg ggcttaatgt catccatcat
     5101 tgattgttta taactagtcg ctttgttatc ttctatatta aagttcaaca tcgctttact
     5161 gttatttaaa atcggaaaat catcatacgt ttgttcatat tttacagaat ctgaactaat
     5221 ctcgcttaat tgatattcag cacctttaaa tacgcgcttg ttaacataat ctttcaaatc
     5281 ttttaagtta ttgtcactga cctttaccga ttgactaatg ttcgcattca ataattttcc
     5341 accatctgat gtggtcaaat ccgaatcgcc tttagcttta gaactaaagt cttttgatcg
     5401 ccctgtaatt tgctctaatt ttataccttt aactgattta ttcaatatac tagtcggtac
     5461 tttaatttct tcttgctgaa aattaacttc attgttactc tcgacttcat taatgtgtga
     5521 gcgattgact ttattaacat aaatcgatac taacacgatg ttgacaagaa taaacacgaa
     5581 aatgaaaagt gtctttgtca gtttccagtt catttattca agcctcccat cgttataaac
     5641 ataccattcg ccatcatatt ctacatacca acgcggtacg agttcactgt taatctgcac
     5701 ttcaatgtga ttatgatctg aattatcctg catttcgtaa ccgatagcga tgtttgttac
     5761 tttttcaaaa ttaatatcac tattgttcgc taagcttgaa cgtacagact ctaatttcgg
     5821 caacgattta ttatcctcac tatttaaaac aacgtcggtg cgtaataacg aacgacgata
     5881 gtcaaagacg cctttttcac cccaagtgac ttgaatttga ttagaacctt ctttattaaa
     5941 cgttggataa ccattaagga aacgttgata tgttaactcg cctgactgat tattcgtact
     6001 aaacaatcta aagtcttcgt ttaagaaacc accatgacca ttaataaaat caaaggttcc
     6061 tggaatcgtt tcttccattt tgctggaact cgcttcatct tcggacaggt ttttataatg
     6121 atatttttca tttttatcgt tatagtttgc gacacctgta ttattgttgt aggttgtaac
     6181 accactcttt gaactacgaa cgatggttga atcgtcaaat agtatagcat tcattttctc
     6241 aacactaatc gtgttaaata ccatgcgata tgtttttaac ttttcaggtt tacttggtgc
     6301 aaaaacatgc gtcgtacgat caattgtatc tttgtttgtg atgatatctg tgtatggttg
     6361 catatctttt ttcactgctg ctaatgcatc taaaaaatga tcatttttcg ttgtagttgt
     6421 taattttacg taatcgtggc gatctttgct tatagcataa agcacgatat tatcatcagc
     6481 atcatgatct atgaccaaac gattgaaatt gaaatgattt ggtactttcg cattcatgtt
     6541 cagtacttga ccaagatatg ttgataacgg taaatcatac gtaaaatcga ataatataaa
     6601 atcactgttc aaatcaggaa tcatcaagtt atgatcacga cgaacatgtt ccacggactt
     6661 cacttcttta tttttcaaag gtttcgtcag tttattcaca ttagataccg tcgcaatcgt
     6721 tccttctgga tgatcatttt tcgaatgaat aatctgaaat ggcgtaatag ttgtatccat
     6781 tttggctgtc ataggtgtcg ttaaaggttt cgtttcactc ttcttactat ctgtattgtc
     6841 gacatttgca atatcaggag aaaagttcca taccatatat gtcaatacga cactcatcaa
     6901 gacgagtagt gctaaaatga cagatttaat atgctcctta ttattcatcc caatcaccgt
     6961 cttcaatgac ttcacatgga agtgtgataa agatagatgt accttgacct tctacactgt
     7021 ttgcccaaat acgaccattg tgcgcttcca caatctcttt cgaaatggct agtcctaatc
     7081 cagtaccacc cattttacgc gtacgtgcct tatctacacg atagaatcgg tcgaatatct
     7141 tatcgacttt attgatagga ataccaatgc cattatcttt aatacgaatc gtcattcgat
     7201 tataaagtgg attttgtttc acgtggaact cgacacgttt atcgcctcta gaatatttca
     7261 tcgcatttgt aatgacatta tcaaatactt gcgtcatttt atcaggatca aattctgtga
     7321 aaatcgtctt tttcggaata tctcgaataa atgttgtatc tttcgcagac atttcatgtc
     7381 gattaataat tttattaatg aacatgttaa agtcgataat ttctttgttg atttgatcag
     7441 actcattatc cattttagat aactgtagca agtcattgac cagtcgaatc attcgttctg
     7501 tttcttcacg ggtaacagat aaaaattgtg gcgcaagttc ctcatctttc catgcacctt
     7561 cttcaagtgc ttcaatgtaa ctattcatag aagttaaagg tgtacgtaac tcatgtgata
     7621 cattggcaac aaattcacga cgctcacgtt caacttgttg ttgttcagtt acgtcatgta
     7681 acacagcgat ataaccagtt acaaatcctg tttcctgcac aatcgtacta aagttaacac
     7741 gtgcgattag accttcttct tcatttaaat ctaataagaa actatcatta ttctcttgaa
     7801 tttcttccag tttaaattca tcttcaagac ttaatacact taacatgtaa tatccgatga
     7861 tgtcttcttt cgccatacca agcatcttga gtgccatatc attgacgata cgaatacgtc
     7921 cacggcggtc tgttgcaata ataccatcac tcatatgggt gataactgag tccagtctac
     7981 gtttctcact ttcagtatta gcctgcgctt cttgtacacg tttagacaag ttattaaatg
     8041 ctaaagctaa ttcgccaatt tcatcattac cataaatctt cacacgttgc gtatagttac
     8101 ctctggacat ttcgaccgtc tggttacgca tatcggtgat tggtttggta atcgttcgcg
     8161 ctataaagaa tcctaggatg actgtgatta ataatgaaat agctgtacca acaatgaata
     8221 tttgatttat attatttaat tggttataaa cgtcattaat ttttgattcg atataaatat
     8281 taccaattac ctttttatcg actttaactg ggatattata tacccagaca cggtccttac
     8341 caccgccata atcttttaaa attaaatgat cgtttgattg tcctagtgat agtgcttttt
     8401 ggacagaact atcattcgct ttttgattga ttagactacg gttagactgc ttcgtcgtcg
     8461 caataataat ttggtcttta tctataaaac gaatttctcc aatttcttga cggttggcat
     8521 actcacttaa taaattttga atatcttttt gtgcatttac ggagcccttt tcgtcatata
     8581 ctttttcaat actaatttct aattgtttcg cgtactgcgt aatattcttc ttaaaattat
     8641 caagcagctc tttttcaagg ttatttgtaa aatataaccc gataatttgc ataccaatga
     8701 taatcagtaa tacataaaca attacaagtt tagtatgaag ggattgtagt tgttttagcc
     8761 acttcattcg tttcgacctc tactcatgtt gttggaggaa atatccaacg cctctacgcg
     8821 tcacaatata ttcaggatgt gacggatcat cttcaatctt ttcacgtaaa cgacgaatcg
     8881 ttacatcgac cgtacgtaca tcgccaaagt aatcatagcc ccatactgtt tgtaataaat
     8941 gttcacgtgt cattacttgt cccatatgtt ttgataaata atggaacaat tcaaattcac
     9001 gatgtgttaa ttcaatatct tcgccacgtt ttttaataga atatgcgtct ggataaatca
     9061 caatatcttt aattgtgatt tcattcgtta catttccagt gtcttgtgct ggttgtgagt
     9121 aatgacgacg taagttcgct ttcacacgtg cgattaattc acgcgtacta aacggtttcg
     9181 ttacatagtc atctgcacct agttctaaac caagcacttt atcaatttct gaatctttag
     9241 cagtaagcat tattattggc atttcgtatt ttttgcgcac ttcacgacat acttccatac
     9301 catcacgacc aggtaacatg atatctagta atacgatgtc tggttcttct tcataaatta
     9361 agtcgactgc atcattacca tcgtatgcac agtacacatc gtatccttct ttttttaagt
     9421 taaattctaa aatatcagca atcggttttt catcatcaac tacaacaact tttctagcca
     9481 tttgcataaa cctcttttct taaatcatta atattatttt atcatttttt gttctttatc
     9541 gtttatgttt gtgtaaaaaa tcacaggatg tcgcttacta tttttgttat tgtgcgcatt
     9601 tt
//
LOCUS       DAKTGS010000111         9434 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.111, whole genome shotgun sequence.
ACCESSION   DAKTGS010000111 DAKTGS010000000
VERSION     DAKTGS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.111"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            47..1465
                     /locus_tag="O0286_002141"
     CDS             47..1465
                     /locus_tag="O0286_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCY0170505.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(1878..2786)
                     /gene="rarD"
                     /locus_tag="O0286_002142"
     CDS             complement(1878..2786)
                     /gene="rarD"
                     /locus_tag="O0286_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCY0170506.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            3011..3586
                     /locus_tag="O0286_002143"
     CDS             3011..3586
                     /locus_tag="O0286_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0170507.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            3751..4767
                     /locus_tag="O0286_002144"
     CDS             3751..4767
                     /locus_tag="O0286_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCY0170508.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            5010..5804
                     /locus_tag="O0286_002145"
     CDS             5010..5804
                     /locus_tag="O0286_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCY0170509.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(5938..6450)
                     /locus_tag="O0286_002146"
     CDS             complement(5938..6450)
                     /locus_tag="O0286_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCY0170510.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            6733..7491
                     /gene="vraD"
                     /locus_tag="O0286_002147"
     CDS             6733..7491
                     /gene="vraD"
                     /locus_tag="O0286_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCY0170511.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7481..9361
                     /gene="vraE"
                     /locus_tag="O0286_002148"
     CDS             7481..9361
                     /gene="vraE"
                     /locus_tag="O0286_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCY0170512.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
BASE COUNT         3141 a         1620 c         1504 g         3169 t
ORIGIN      
        1 cttaaattga aatatgtata aaacatagaa agaaggacgt tttaacatgg aaaacacggt
       61 taaatatcgt aagtttatac tccctatcgt tgtaggtctc cttatttggg cacttacacc
      121 ttttaaaccg gatgctgtgg atccaacagc atggtatatg ttcgcaatat tcgtcgcgac
      181 aatcattgct tgtattacac aaccgatgcc aattggggcc gtctctataa ttggatttac
      241 aatcatggta ctcgttggca ttgttgacat gaaaacggct gtcgctggtt ttggtaataa
      301 tagcatttgg ttaattgcta tggcattttt catttcgaga ggatttgtga aaacaggtct
      361 tggtagacgt atcgcacttc atttcgtcaa attatttggt aaaaaaacat taggattagc
      421 atattctatc gtcggtgtag atttaattct agcgcctgct acaccaagta ataccgcgcg
      481 tgctggtgga atcatgttcc caattatcaa atcactttct gaatcatttg gttcgaaacc
      541 gaaagacgga tcagcacgca aaatgggtgc atttcttgtt ttcacagaat tccaaggtaa
      601 tttaattact gcggctatgt ttttaactgc aatggccggt aacccccttg cacaaaattt
      661 agcatctagc acatctaatg ttcacattac atggatgaat tggtttctag ctgctttagt
      721 tcctggactt gtttccttaa ttgttgtacc ttttattatt tataaaattt atccaccaac
      781 tgttaaagaa acaccaaatg ctaagagttg ggctgaaaat gaattagcga ctatgggtaa
      841 aatcgcttta gctgaaaaat ttatgattgg tatttttgtc gttgcgttaa cactatggat
      901 tgtcggaagt ttcattcata ttgatgcaac tttaacggcc tttattgcgc tagcattgtt
      961 attattgaca ggcgtcttaa catggcaaga cattttaaac gaaacaggtg cttggaacac
     1021 attagtatgg ttctcagtat tagtgttaat ggccgaccaa ttaaacaagc ttggatttat
     1081 tccttggtta agtaaatcca ttgctacaag tcttggtggc ttaagctggc ctatagtcct
     1141 ggtcatttta atattgttct acttctattc acattactta tttgcaagtt ctacagcaca
     1201 tatcagtgcg atgtatgcag cattactagg cgttgccatc gcagccggtg caccaccatt
     1261 attcagtgca ttaatgttag gtttcttcgg taacctatta gcttcaacaa cacactatag
     1321 tagtggtcca gcgccgattc tattctcttc aggttacgtg actcaaaaac gttggtggac
     1381 aatgaactta atattaggtt tcgtctactt tattatctgg attggtttag gatcactttg
     1441 gatgaaagta attggtatat tttaaaatat ttaaattagc gctcgaatct cattgatttg
     1501 ggcgcttttt aatttgtatt taaaatcaac ctttgctaaa tcaagactcc ctttttaaaa
     1561 tacgtttatc ctttaaatca ttgcgtgctt cactgaaaat ttgtataaag atttaagtca
     1621 ttacgtaaca tcacataaaa tacatttcta tactattccg cttcattgat taacattacg
     1681 tatgccctca taaatcatca tacaaaaaac accttcgttt aaattcattt taattgcgaa
     1741 ttcaacgaaa gtgccttatt tcatatttaa tgtttcaaat ttatacgtct gtcactgtta
     1801 ctgcacacat acctcagtct cacctcatca tgacacaaag gatattgaaa acacgtataa
     1861 ttatctttcc tcgtatttta tagggttttt gcgaccggat gtttcttcaa tttaatgtat
     1921 tgagaaagac tatataacac aatacctgtc caaataaata taaacgtaat taattgatct
     1981 atactaaaag gctctttgaa aacaaatatg ccgagtacaa acattattgt tggtccaacg
     2041 tattgaataa atcctattag cgaaagtgga atacgttttg ccccggctga gaataggatt
     2101 agtggtattg ccgtaatagc accagaaaat aacaaccaaa atgatgacat gttcaatcca
     2161 aatgacatct gatgttgctg ccataaataa ataacgtata ttagtccagc aggtgcggta
     2221 acaatacatt caatcgtaat actgctgatg gcatcaatat gtactacttt tttcaataat
     2281 ccgtatgtac caaaggataa cgctaatata atagagacga ttgggaattc tccaatcttg
     2341 agcgtcatat ataatacacc gatgaatgcg aataaaatgg ctagccattc aaatttattg
     2401 aatctttctt ttaaaaagat aagtgcgagc aaaatgctaa caagtggatt tatataataa
     2461 cctaaacttg tttgtaggac gtgaccgttc gttacagccc aaataaatgt accccaattt
     2521 aatgtaatga catagcctgc tacgacaatc gctaatagct gaatgggctt gcctaacaat
     2581 tgattcatat ctcgttgaaa tgcattgcgt tgtttttgtc caaccgcgag tatgaaaatc
     2641 atgaatattg ctgaaaatat aatacgaaag gctaaaattt caaatgcgcc tattgcatca
     2701 acgaactgcc aatatatagg tagtattccc cacagaatgt atgcactgag tgctaaaaat
     2761 atgccttttt tatactctga attcaccttc aaacctcctt actttcctaa tttttaattt
     2821 actgcatacg ctcacttggt tatgctaata taacgatttt actaataata tttcgataaa
     2881 gatatcattt tgtttatatt tcccacattt attcaccaac cactaaacaa tattaatttt
     2941 ataaataatt ctgtacaaat cagggtatat tgccagaaag actaccatac aacataaagg
     3001 atggatacaa atgactttac ctaaaattgg aaagcctgca acacgcgcgc taaattcaca
     3061 aggtatatac acattagaag cagtatcaca atatacgaag tcatctctaa tggagatgca
     3121 tggcgttggt cctaaagcta tatcaatatt ggaacaagct ttatttcagc accaactaca
     3181 ttttaaaacg gaagttcagt catcattacc ttttaagtta acaggagatg tgtcatgcaa
     3241 tcatgcgcct aaacgtcaac aaatgataga ttttatagtt gttacagcag cattagatat
     3301 tgaattatta cgttcactgg ttacaacaga atttatttgg tctgtccctg gccgctttga
     3361 catatacgga cctcaaatat taattcaaga actatctaat cattacaatc aggttgctag
     3421 tctcaatatc cattccagca tcacacatgg gtgcttaggt tccatgcatg gtattgaaat
     3481 attaaaaact ggtaaagaga ttcattttgc tcattttttt gaatttgaaa atcataaaaa
     3541 ggatgccaag ttaagcaaag tgacatctta cattgttgtt ggttaattta tcaaattata
     3601 tatatcgcaa aaaatctttt gtggttacat gtatattttt atataacaaa taatttggca
     3661 taacattttt gttatatgaa cacttattac tagagtgccc attgaacata cagtatgatt
     3721 aaatatcatg ttatgaaaga aggatatatt ttgactgttt ttaaaaatga gcgcttaagc
     3781 tggttaccat acatagctat tgtcattttg ttacacgtta ttgggtttag ttttttatgg
     3841 attgctggaa aagaccatca tatcttattt ggtatgggga ttcttgcata tacattaggt
     3901 ttgcgtcatg catttgatgc agatcacatt gctgcaatag ataatacggt tcgcaaatta
     3961 ttacagcaac gcaaagatcc atctggtgtg ggtttctatt tttcaattgg acattcatct
     4021 gtcgtatttt taatggctgt ttttttaggg gtatctgtaa aatgggctaa agatgaatta
     4081 ccgcatttcc aagatattgg tggaacgatt ggtacactag tttcaggttt ctttttagtg
     4141 cttatcggtg tgttgaatct aattatttta atctctttaa ttaacttgtt cgctaaatta
     4201 cgtcgtgaac acattgaaga agctgaagtc gatgcattac ttgaatctag aggattggtt
     4261 tctcgatttg taggacctta tttcaaatta atcacgcgta gttggcacgt attgccactt
     4321 ggctttttat ttggacttgg ttttgataca gctagtgaaa ttgcgttact cgctctttct
     4381 tcaggcgcat cacaacaagc catttcattt atcggaatct tatctttacc aattttattc
     4441 gcatcaggta tgagtttatt ggatacatta gatggtgttg taatgaagta tgcctataat
     4501 tgggcattct tcaatcctat tcgcaaaatc tattacaata taacgattac tgcgatatca
     4561 gtcatggcag cattagtgat tgggatgatt gaattgctac aaattcttgc tgataagtta
     4621 gatttacatg gtgcgttttg ggcattcatt ggttcgatcg aatttgatta tttaggctat
     4681 attttagttg cattattttt aattacttgg cttatttcaa gtttaatttg gaagtttggt
     4741 cgcatagaac acaaatggtc tagataaggc aacttgtctt gcaactacct ttgtattgtt
     4801 gcatttttag acaccctgca cacttcttcc attatgctac gctctcagta gtcatcttta
     4861 aatcgtttct tttagagatg acttcatttt gagagcgtat ttttctattt aaaatgtatt
     4921 tatccaaaat tcaatgcttt cgaatttaaa atttcagtat ttctgtttaa aatttaatta
     4981 taatctttaa tacaggaggc gctatgctta tgaatattgc gaagttagag aattatttac
     5041 aaattgattc atctcgttat aatcgaccga gtattgaagc attaaattat tatgcaacac
     5101 gttttatgtt aactgtacct tttgaaaata ttgatgttca aaatagtaag ccgatatcta
     5161 taaatatcga tgcacttttt aataaaattg ttcatgataa acgtggtggt ttttgttatg
     5221 aattaaatac atttttcaaa gcctatttac aacaaaaagg atttaatcct gaattaatgt
     5281 cagctactat tcacacaccc ggtggcggtc gtagtctgaa cggttcacat gcttcacttg
     5341 ttgtttctat aaatgatgta ttctatgtaa ctgatgttgg ctttggagac ttacctctac
     5401 atgccattcc tattacttca tctgagcata cacaaccaat tactgatatc agtggtacat
     5461 tccgcgctat ttttaacaac gaagacaagg atattttcta tgttcaaaaa ttcgaaaatg
     5521 atcattggca tacaaaatat gaagctgaat tcaaacctaa acaaatagaa gattttaata
     5581 gcaacatcga atataatcaa acgcatccag attcaatatt tgtacagcat ttattaatta
     5641 caatgccaca atcatttggc cgtgctacaa tgtctgaaaa tcatttaact ttaacaagga
     5701 atggtagctc agaaaaattt gatgtcacta aagataacta taaacatttt ttagaaaaat
     5761 attttggact aaatgtaact attaatcgta ttgaaaaaca ataatagtaa aatataaaaa
     5821 tatgggcgta tcttacttat tgtaggtagc gccccttttt tatgcttcat aaacacaaca
     5881 aaaaggatat gacacaaact tcatgtcaca cccttctttt gaaccgcttc attttactta
     5941 agcaagtgca tctacaacat aaatgacacc tacgaataca aatgcaatag caatcatcgt
     6001 cgaaataaag acgataccca ttaatggatt aaataataaa atgacaccga aaacaatacc
     6061 taataaatta aaaatcactg aaattagttt taagctacca ctacccgaaa acgtaaataa
     6121 tccagagata gaactaaaaa taaaccaaaa agcaaacata taaataaaga atgctgaact
     6181 tgcgccaaca ttaaaaataa ctaatagacc aaatagaata tctacaatcc ccataaacag
     6241 aatccaattt tggttaccac ctactaaagc ttttgctttt ctacggtaaa cgatttgaat
     6301 cacaccgtta attaatacaa acagtccaat caaccaggta atagcataaa aattttcaat
     6361 tggaaatgta aaaataacga ctgccaacat taataataat acacccatta ttaaactaga
     6421 ccatttaata ccttgatttc ctttttccat aacaatcaat tcctttcaaa taaaaccctc
     6481 ttttaatttt tacaaaaaat ttattataca ataattaaat tcccttttct caatttacac
     6541 aaacatcatc acttagaaag caccattgct tttgtctaat atgtacgatg aatctgtatc
     6601 ctcactcaaa aatctactaa gtagcgatag ctttcaactc tgtaaggttc attcattgaa
     6661 ttgtaagttt agataaaggc tactactcca aacattattt aaaatgaaat taaatacaaa
     6721 aggagtgaga ctatgacgat attatcagtg caacatgttt caaaaacata cggtaaaaag
     6781 cacacatttc aagcacttaa agatattaac tttgacatac aaaaaggcga attcgttgcg
     6841 attatggggc cttctggatc aggtaagaca accttattaa atgtactaag ttcaattgac
     6901 caaatttcta gcggtagcgt gattgctaac ggacaagagc ttaataaact taatcaaaaa
     6961 gcacttgcca aattccgcaa agaatcatta ggtttcatct tccaagatta cagtattctg
     7021 ccgacattaa ccgttaaaga aaacattatg ttacctttat ctgttcaaaa aatgtcgaag
     7081 gcaacaatgg aagaaaatta taaagcgatc acgacagcat taggtattta tgacctagga
     7141 aataaatacc ctagcgaatt atctggtggt caacaacaaa gaactgcagc agcgagagca
     7201 tttgttcaca aaccacaaat catatttgca gatgagccaa caggcgcact cgactcgaaa
     7261 agtgcaaatg acctattaca acgtttggaa gaaatgaata aatcgtttga tacaactatt
     7321 gtcatggtta cacatgatcc ggttgcagca agttttgcag aacgtgtcat catgctaaaa
     7381 gatggccaaa ttcatacaca actttatcag gaaggacgtt ctaaacaggc cttttatgaa
     7441 gacattgtac atcttcaatc agtattaggt ggtgtctcaa atgacattta accatatcgt
     7501 tttcaaaaac ttacgacaaa acttaaaaca ttatgcaatg tatttatttt cattattttt
     7561 tagcatcgtc ttatatttca gttttacaac cttacagttt actaaaggtg taaataatga
     7621 cgactcgatg gccatcatta agaaaggtgc tttagtcgga tcaatatttt tatttatcat
     7681 tattgtcatc tttttaatgt atgccaatca tttattcgta aaacgccgta cacgtgaatt
     7741 tgcgctattt cagttgattg gtttaacacg acaaaacatt ttgaaaatgt tagcacttga
     7801 acaaatgatc gtatttttaa tcacaggtgt tgtcggtgta ttatgtggca ttgcaggtgc
     7861 acaattattg ctttccatag tatctaaatt gatgtcgtta tcgattaact tatcgataca
     7921 tttcgaacct atggcccttg ttttaactat tttcatgcta attattgcgt atgtactgat
     7981 tttatttaaa agtgctttat ttctaaaaag acgtagtatc ttatcaatga tgaaagattc
     8041 aattaaaact gatgccacaa ctgctaaagt aacgactgca gaggttattt caggcgtatt
     8101 aggtattgct atgattgcac taggctatta tatggcgaca gaaatgtttg gtacgttcaa
     8161 agcactaact atggctatga catcaccgtt tatcatttta tttttaacgg ttgtaggcgc
     8221 ctatttattc ttcagaagtt ccgtgtcact tatttttaaa acattgaaaa aatcaaaaaa
     8281 tggacgcgta tctattaccg atgttgtatt cacatcgtct attatgtaca gaatgaagaa
     8341 aaatgccatg tctttaacta tcattgcaat tatttctgca gttacagtaa ctgtattgtg
     8401 ttttgcggca ttatctaaat caaatacaga tcaaaccctt acatctatgg caccaaatga
     8461 atttaacgtg gtcgctactc aagatgctaa acaatttgaa actaaactaa gccaacaaca
     8521 aattacattt tctaaaaatg cttacgaaac tattactgta gataatgtta atgatcaggt
     8581 tattactttg gaaaatggta gtgattcagg tcgcacgaat tctattttaa gtgcaaataa
     8641 caagttaaca ggcaacaatg ccatcattac aaatacaaaa tcgctgccta acattattaa
     8701 tatccattta aacaaagatt tagtagtaaa aggtactaag aatgaaactt tccgtgttac
     8761 acaagaagac aaaggtaagg tttatccttt aaatctaagc ttcaactcac ctgtcatcga
     8821 agtaagtcct gaaaagtatc agcagttgaa aacacaaaat aacgttcata ctttttatgg
     8881 atatgatatt aaacaaacat cacaaaagga aaaagctcaa gcaattgcaa aacagtttgg
     8941 agacaaagtc atcacgtatg atgaaatgaa aaaagaggtc gatgctacta acggtattct
     9001 aatatttgtt acatcatttt taggattagc atttttagta gcagctggat gtattattta
     9061 tattaaacaa atggatgaaa ctgaggatga actaagtaac ttcagaatat taaaacgcat
     9121 aggctttacg catacagata tgcttaaggg attattatta aaaattactt tcaacttcgg
     9181 tttaccatta ttgattgcta tattacatgc agtattcgcc gcaatagcat ttatgaaatt
     9241 gatgggtaat atttccttca tgccggttat tgtagtaatt gttgtataca ccctcattta
     9301 cattactttt gctttaattg cttttgtaca ttcaaacaaa ttgattaaga aaaccattta
     9361 acacttacga ttaaaagatg cttcattaga tattttgatt ttaagcaatg cagtataact
     9421 attaattgat ttaa
//
LOCUS       DAKTGS010000112         9433 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.112, whole genome shotgun sequence.
ACCESSION   DAKTGS010000112 DAKTGS010000000
VERSION     DAKTGS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.112"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..223
                     /locus_tag="O0286_002149"
     CDS             <1..223
                     /locus_tag="O0286_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0170513.1"
                     /translation="LEPWTSCEFDFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKK
                     FGIWPEKEYAINWVEKIKDYVKEQDEAEL"
     gene            234..734
                     /locus_tag="O0286_002150"
     CDS             234..734
                     /locus_tag="O0286_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0170514.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            745..1245
                     /locus_tag="O0286_002151"
     CDS             745..1245
                     /locus_tag="O0286_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0170515.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKE
                     QDEAEI"
     gene            1256..1744
                     /locus_tag="O0286_002152"
     CDS             1256..1744
                     /locus_tag="O0286_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0170516.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
     gene            2367..2741
                     /locus_tag="O0286_002153"
     CDS             2367..2741
                     /locus_tag="O0286_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0170517.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            2894..3292
                     /locus_tag="O0286_002154"
     CDS             2894..3292
                     /locus_tag="O0286_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0170518.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(3508..4332)
                     /locus_tag="O0286_002155"
     CDS             complement(3508..4332)
                     /locus_tag="O0286_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0170519.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(4569..5876)
                     /gene="brnQ"
                     /locus_tag="O0286_002156"
     CDS             complement(4569..5876)
                     /gene="brnQ"
                     /locus_tag="O0286_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0170520.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            6460..7350
                     /locus_tag="O0286_002157"
     CDS             6460..7350
                     /locus_tag="O0286_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCY0170521.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            7599..8648
                     /locus_tag="O0286_002158"
     CDS             7599..8648
                     /locus_tag="O0286_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCY0170522.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            8661..9338
                     /locus_tag="O0286_002159"
     CDS             8661..9338
                     /locus_tag="O0286_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170523.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
BASE COUNT         3615 a         1254 c         1684 g         2880 t
ORIGIN      
        1 gcttgaacca tggacatcat gcgaatttga ctttacaaga gatggcaaat tgaatgtatc
       61 ttttgattat attgattggg tgaattcaga atttggacca atgggaagag aacattatta
      121 tatgtataaa aaatttggaa tttggcctga aaaagaatat gccataaatt gggttgaaaa
      181 aataaaagat tatgttaaag agcaagatga agctgaacta taggggagat aatatgactt
      241 tcgaaggaaa actaagtcaa atgtacaacg agattgcgaa tgagattagt gggatgatac
      301 cggtagaatg ggagcaagta tttacaatag cttatgtaac tgatcaagct ggagaagtca
      361 tctttaatta tactaaacca gatagtgatg aattaaatta ttattcagac atacctaaag
      421 attgcaatgt ctcaaaagat atttttaaga attcatggtt taaagtttat cgaatgtttg
      481 atgagttaag agaaactttt aaagaagaag ggcttgaacc atggacatca tgcgaatttg
      541 actttacaag agatggcaaa ttgaatgtat cttttgatta tatagattgg ataaatacag
      601 agtttgatca attgggccgt caaaattatt atatgtacaa aaaatttggg gttataccag
      661 aaatggaata tgaaatggaa gaagttaaag aaatcgaaca atatattaaa gagcaagaag
      721 aagctgaaca atagaggtga taacatgact ttcgaagaga aaataagcaa attatataat
      781 gagattgcga atgagattag cagtatgata ccggtagagt gggaaaaagt atatacaatg
      841 gcttatatag atgatggagg aggtgaagta ttctttaatt atactaaacc aggtagtgat
      901 gacttgaatt attacaccga tatacctaag gagtataaca tctctgtgca agtatttgat
      961 gatttatgga tggatttata tgatttgttt gaggaattaa gagatttatt taaagaagaa
     1021 ggacttgaac catggacatc atgtgaattt gactttacaa gcgaaggtaa attaaaagtt
     1081 tcatttgatt atatagattg gataaataca gagtttgatc aattaggccg tgaaaattat
     1141 tatatgtata aaaaatttgg ggttttacca gaaatggaat atgaaatgga agaaattaaa
     1201 gaaatcgatc aatatattaa agagcaagat gaagctgaaa tataggggag ataacatgac
     1261 tttcgaagaa aagctaagtc aaatgtacaa tgaaattgca aatgaaatca gtggaatgat
     1321 accagtagaa tgggaaaaag tatatacaat tgcctacgta gatgatgaag gtggagaggt
     1381 tgtttttaat tatactaaac caggaagtga agatttgaat tattattcag atattcctaa
     1441 agattgcaat gtctcaaaag atatttttaa gaattcatgg tttaaagttt atcgaatgtt
     1501 tgatgagtta agagaaactt ttaaaaaaga agatttagaa ccgtggacat catgtgaatt
     1561 tgactttaca agaaagggaa atttaaaagt atcatttgat tatatagatt ggattaaatt
     1621 aggttttggc ccatcaggaa aggaaaacta ctatatgtac aaaaaatttg gtattttacc
     1681 agatatggaa tatgaaatgg aagaaattcg agcagtagag aagtatgtta aagagcaaga
     1741 gtagcagaca tgttataaaa gactgtgcaa aatcaccctc gttttacatt tgattcaaag
     1801 aagaaggtaa aagataagat tatttgcaac ttaaaaagtc aattagctta tcggtatcca
     1861 tacatcatgg ataaatgagt tcaactaatt aacaaatcac gatataaatt taaaattgtc
     1921 attaattgaa aaggttattg tagtgtattt tataaagttg atgtacatcg acttattttt
     1981 taaaacttat atcaaaaaaa tatgagaaaa gtatatacta ttaaaatgtt ttttactata
     2041 attataaata gttaaatatt tgggaattaa ataaatatat aggaggcaat ttttagagat
     2101 acatagcaat gtaaaggtat caaatagtta tttcataatt tattatgatg acataatttt
     2161 cagtagatag ttgtaaatta acactgaaat agatgtttat attaagaaga ctttaatatc
     2221 ataattaatc aaaatcttgc gtcaaacatt taaacgaatt ttaagttgat aacaattggc
     2281 aaaacattgt tcaaacatca caatgataaa gcatattatc agtattgtag tgtgtggaaa
     2341 atgacagcca tctaaggaga aaaatgatga aaagaatatt ggtagtattt ttaatgttag
     2401 caattatatt ggcaggttgt tctaataaag gtgaaaagta tcaaaaagat attgataaag
     2461 tgtacaaaga acagaatcaa atgaataaaa ttgcctcgaa agtacaaaac actattaaaa
     2521 cagacattaa acaagaagac agtaatacac atgtttataa agatggtaaa gtcattgtta
     2581 ttggtattca attatataaa gatcgtgaaa aaatgtatta tttcgcatat gaaataaaag
     2641 atggtaaggc agagattaat agagaaatag acccaattaa gtatatgaaa gaccataaag
     2701 cagattatga agatgaaaat gtagaagtgg aaaaagatta acataggctt tctttaaaac
     2761 ttttaaccag tttcaaaaag taaatgagct ttagcgacaa taatttttaa aataaacacg
     2821 atataaattt aaacatttat atataagaac acaaacgcaa gtttgtaaat aataataata
     2881 aaggagtaag acaatggaaa aatcgatcaa aataatgaca ataataggaa ttgttgttca
     2941 gggtttagca acggtattta gtttactatt gatggtttta gcagcatcag gtgtaatgac
     3001 tacagatgtg tcaacaacag ttaatggtga ggttgaccca gttgatgcag aaacagcagc
     3061 agcaattttc actgtattat tcttatccct attcatattt ggaatcattt caattatttt
     3121 aggtgcaatc ggtatgttta aagcatctaa aaacaaaaaa atgagtggta tattgttgat
     3181 tattggagct gtaataagtg gtaacataat tacatttgct ttatggttaa tcagtggtat
     3241 taaactactt actaataaca agcctaaaga tgaaataagc gacttatcat aaacatcgta
     3301 tattgaaatt tcaagatttc ttaagtaatt aaataaagtg ctctcttaga agtagatttt
     3361 cagtcaataa ctgcttttaa gagagcattt tgtatttgat aatgctcata gcatcggata
     3421 aaaatcattg acgaacttta aaaaaatacg ttagacaatg cacttctaaa tattaaaggc
     3481 atcgactaac gcattgttca aatatagtta attattttta taaaattgat gatgatcatt
     3541 caagtaagca tagaataaac ctataatgag tccgcctcca atatagttac cgataaaagc
     3601 cgcagcgata ttcgaaatag ctggtatgaa gtgcaatgta tcaacttgat aaattaaacc
     3661 acccataaat aagcaactgt tgtaaacgac gtgttcataa cccataaagg cgaatatggt
     3721 aacaccgaac atcatgacaa acatttttgc gagtacatcg tcaatttgca tggcaataac
     3781 taatgaaata ttgataaaga aattggcgaa gatcgctttc attaatatac ttacaaaacc
     3841 agtagacaac gttttatgct ctataactgc tgataactga tttaacatat ctggcgtcat
     3901 tacatttgaa aaacgcatga aactaaataa aatagcagca cctaaaatat ttcctgcaaa
     3961 gcataataaa aatattttca atactctagt tggtttaatt actttataat acaggcctac
     4021 agtaaagtac atgaagttac tggttagtag ttcggagttt gtaaataaaa tgagtactaa
     4081 cgcaaagctg aatgtaatgg cactggccat attcacaatg cctggcggta aatctggttc
     4141 gtgtgttgct ttaactgata atacgaagac cgtaataatc ccgataataa atcctgccat
     4201 catcgcgcgt aataaataac gttttaaata aacgctttgt aatatatctt ttgttcttat
     4261 cgtttcgact acgttattta cccagtcgtc cccataaaaa atcttatccc atttaatatg
     4321 tttctccttc acaatgacat cctcactttt catttgttaa tcagagtata ctatgaattg
     4381 ccttatttgt gaataatttc acaaaattaa ttttaaaaac taatattttt gaataacagc
     4441 taggattttt taacaaatca tttattttaa aataatcaat agacagacgg tatcactgaa
     4501 aagtcatgaa tttcatataa aaaagcctag taacgatggg tcaatcatta ctaggcagtg
     4561 agcatttatt aagttgtcgc ttgtttcgga cggcgtataa atacatcgat tatgaaaccg
     4621 ataatagcaa agagcatgaa tggtacaagc caagctaaat cgatatctgc taaaggtaac
     4681 atcataaacg atttcaaaat aacaccgtgt aataagttga aactatttag tatttgtaaa
     4741 attgaaataa tcaatgtaat aacagttgcg agtcgatagg cccaactgaa tctgaatgtg
     4801 ctaaacatgt tagcaaatga tatgagtaca agtgcaatcg acacgggata tattaaagtc
     4861 aataatggga cagcaatttt taaaatcatt tctaaaccaa gtgttgtaaa taagaaccct
     4921 atgatagaga aaataagtgc gaatatttta taagaaaact taggtacgtg tttcttagta
     4981 aatgtggcgc aagcattgac gagtcctata catgttgtta ggcatgcaag gataaccgtc
     5041 attccaaata cgaggttacc gaacgaacca aataatcgta atgagttgta cgtcaatata
     5101 tctgtaccat ctttaaagtt tcctggagct gttgatgccc caacgtatgc aagtgcaaag
     5161 taaatcattc caagtaatat ggctgcaata agacctgaaa agcagacata ttttaaaatt
     5221 ttcatgcgat ctgtgaggcc tttaaactta tagccattga caatgactac ggaaaaagct
     5281 aacgcagcaa caagatccat tgtaaaatag ccttccaaac ttcctgaaat gaaaggatgt
     5341 gttatatatt tatccttagg tgcacttagt gcagattcag ggttgaaaat gacagcaata
     5401 cttaatagag cgaccattaa tagtaataac ggtgttaata atttacctaa attatcaacg
     5461 attttcgatg gatttaaact aatccagtaa acgatggcaa aaaagattgc tgcgaatata
     5521 attaaagtcc attggttatg cacaggtaaa atgtgtcttg taccaatttc gtacgcgaca
     5581 tttgcagcac gtggaatacc gtaaaatgct ccgatagaca tgtaaatcac gacagcaaaa
     5641 ataaacccga accatggatg tatacgattg cctacacttt caacaccttc atcataaaat
     5701 gcaacaacaa taacagtaat aaaggggagt aatatgcctg taagggcaaa gcctagcata
     5761 ccaatccaca tattttgacc cgctgtatgg ccaagcatgg gcgggaatat taaatttccg
     5821 gctccaaaaa atagtgaaaa taacatgagg cccgaaataa taacttgttt tttcaatgta
     5881 atttccttcc taaaactaga tattcataat aatttaaaaa atctgaaaaa taaaaacgtt
     5941 cttactttat ctttaaatga caatactatg attctattat tttataaaaa agattgcaat
     6001 aataacgtct tgctattatt ggataatgac aattaaagtg tcgtttttat gaattaaaat
     6061 aggtgaaagt aagttggaaa tacgttggag cgttgtgatt tttatgagaa attgtatgag
     6121 tgaggactgc tatttataaa aggtaaacta tggatgagtt tatggttgtt ccaataggta
     6181 aataagagat agcacactgt acacataatg atacgtggat gagtgaatat gtctgatagg
     6241 aaggcctagt cactcaaatg aatttaagtt tattacgtga taaatcacaa atctctctca
     6301 tgtgataggt ctcccattaa atcatgctta tatgaaatgt tcacatattt gttagctttt
     6361 caagaaataa tattaaatat cttttcatag tatagcaata ttaaataaat gcatttataa
     6421 ttttaactgt attttaaata ttaatcatga ggtgataaga tgaataaaat ttcaaagtat
     6481 attgcaatag catcattatc ggtagcggtt acagtttcag caccacaaac gacaaattct
     6541 acagcgtttg ccaaaagttc tgctgaagtt caacaaacgc aacaagcttc tataccagca
     6601 tcacaaaagg cgaatcttgg taatcaaaat attatggcag tggcttggta tcaaaattca
     6661 gctgaagcaa aagcattata tttacaaggt tataacagtg caaagacaca gttagataaa
     6721 gagattaaaa agaataaagg taaacataag ttagctattg ctttggattt agatgaaaca
     6781 gttttagata attctccata tcaaggctat gcatcaatac ataataaacc tttcccagaa
     6841 ggttggcatg aatgggtaca agctgctaaa gctaaacctg tctatggcgc aaaagaattc
     6901 ttgaaatatg ctgacaaaaa aggtgtcgat atctactata tttctgatag agataaagaa
     6961 aaagatttaa aggcaacaca aaagaactta aaacaacaag gtatccctca agctaagaag
     7021 agtcatattt tactaaaagg taaagatgat aagagtaaag aatcacgcag acaaatggtt
     7081 caaaaggatc ataaacttgt catgctattt ggagataatt tattagactt tacagatcca
     7141 aaagaagcta cagctgaatc tcgtgaagca ttaattgaaa aacataaaga cgatttcggt
     7201 aagaaatata tcattttccc taacccaatg tatggtagtt gggaagctac gatttacaac
     7261 aataactata aagcaagtga caaagcaaaa gataaattac gtaaaaatgc tattaagcaa
     7321 ttcgatccta aaacaggcga agttaaataa tatatgaatt ggacgtctac atgtacttaa
     7381 aggtatatgt aggcgttttt aaattgatcc ttaattttaa atgtaccgta caaaaataat
     7441 agacaattaa ttgcattttc cataaatttg cttttaatat aaaaagttcg ggtataatga
     7501 aggataagtg tatgtataac attacagagc gcaacacgac gtacgtgcat ctaagaatat
     7561 aaacaatgtt taaatcatag tagtagggag agaaatgcat gtttttagct tggaatgaaa
     7621 tacggcgcaa caaattgaag tttggactaa ttattggtgt gttaacgatg attagttact
     7681 tgctattttt attatctgga ttggcgaatg gtcttatcaa tatgaataaa gaaggcattg
     7741 ataagtggca agcagatgcc attgttctaa ataaagatgc caatcaaact gtgcaacaat
     7801 ctgtttttaa caagaaagat attgaaaata aatacaagaa gcaagctact ttgaagcaaa
     7861 caggggaaat tgtgtctaat ggccatcaaa aagacaatgt tttagtgttc ggtgttgaaa
     7921 agtcatcatt tttagttccg agtttaatag aagggcataa agcgactaaa gataatgaag
     7981 tgttagctga tgaaacgctt aaaaataaag gatttaaaat tggtgacaca ttatcactat
     8041 ctcaatcaga tgaaaaattg catatcgtag gttttacaga aagtgcaaaa tataatgcgt
     8101 catcagtcat tttcacgaat gacgctacca ttgccaagat caatcctaga ttgactggag
     8161 ataaaattaa tgcagttgtt gtacgtgata caaattggaa agacaaaaaa ttaaaccaag
     8221 agcttgaagc ggtaagtatt aatgacttta ttgaaaattt accaggttat aaaccacaga
     8281 acttaacatt aaactttatg atttcattct tatttgtcat ttcagctaca gttataggca
     8341 ttttcctata tgtcatgaca ttacaaaaga cgagtttatt tggcatatta aaagctcaag
     8401 gatttacgaa tggctatttg gcgaatgtgg taatttcgca gacggtcata ttagcactat
     8461 ttggtacggc atttggctta ctgttaacag gcgttacagg tgcattttta cctgatgcag
     8521 tacctgtcaa attcgatgta ctaacattgc tcgtatttgc aattgtgtta atgattgtct
     8581 ctgtattagg aagtttattc tccattttaa caattagaaa aatagatccg ttaaaggcga
     8641 ttgggtagga ggtgtagcaa atgttgaaat ttgaaaatgt aacaaagtca tttaaagatg
     8701 ggaatcgtaa cattgaagcg gttaaagata caaattttga gataaataaa ggtgatatta
     8761 tagcattggt tggaccttct ggctctggta aaagtacatt tctaactatg gcaggtgctt
     8821 tacaaacacc gacatctggg cacattttaa tcaataacca agatattacg acaatgaagc
     8881 aaaaagcatt ggcaaaagtt agaatgtctg aaataggttt tattttacaa gctacaaacc
     8941 ttgtaccatt tttaacggta aagcaacaat ttacattatt gaaaaagaaa aataagaatg
     9001 ttatgtctaa tgaagactat cagcaactta tgtcacaatt aggtctaact tcattgctta
     9061 ataagttacc ttcagaaatt tcaggtggtc agaaacaacg tgtggcgata gccaaagcgt
     9121 tatatacgaa tccgtcgatt attttagcgg atgaacctac cgcggcgtta gatactgaaa
     9181 atgcgattga agtcattaaa attctacgtg atcaagccaa acaaagaaag aaagcatgta
     9241 ttattgttac acatgatgaa cgacttaaag catattgtga tcgttcatat catatgaaag
     9301 atggcgtcct taatcttgaa aatgaaacag tagaatagtt ttattaagcc ggtacatcat
     9361 gtgccggtat ttttatgttt atgtattatt tgaataaact ttcacattca attaataata
     9421 attattatcg aaa
//
LOCUS       DAKTGS010000113         9371 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.113, whole genome shotgun sequence.
ACCESSION   DAKTGS010000113 DAKTGS010000000
VERSION     DAKTGS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.113"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..124)
                     /locus_tag="O0286_002160"
     CDS             complement(<1..124)
                     /locus_tag="O0286_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCY0170524.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNN"
     gene            complement(137..2089)
                     /locus_tag="O0286_002161"
     CDS             complement(137..2089)
                     /locus_tag="O0286_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCY0170525.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(2157..2714)
                     /locus_tag="O0286_002162"
     CDS             complement(2157..2714)
                     /locus_tag="O0286_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCY0170526.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(2740..3906)
                     /locus_tag="O0286_002163"
     CDS             complement(2740..3906)
                     /locus_tag="O0286_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCY0170527.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            complement(3903..4265)
                     /locus_tag="O0286_002164"
     CDS             complement(3903..4265)
                     /locus_tag="O0286_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170528.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            complement(4279..4539)
                     /locus_tag="O0286_002165"
     CDS             complement(4279..4539)
                     /locus_tag="O0286_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0170529.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            complement(4544..4846)
                     /locus_tag="O0286_002166"
     CDS             complement(4544..4846)
                     /locus_tag="O0286_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0170530.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            complement(4839..5156)
                     /locus_tag="O0286_002167"
     CDS             complement(4839..5156)
                     /locus_tag="O0286_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170531.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            complement(5249..5410)
                     /locus_tag="O0286_002168"
                     /pseudo
     CDS             complement(5249..5410)
                     /locus_tag="O0286_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
     gene            complement(5422..5685)
                     /locus_tag="O0286_002169"
     CDS             complement(5422..5685)
                     /locus_tag="O0286_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0170532.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            complement(5710..5925)
                     /locus_tag="O0286_002170"
     CDS             complement(5710..5925)
                     /locus_tag="O0286_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170533.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            5972..6211
                     /locus_tag="O0286_002171"
     CDS             5972..6211
                     /locus_tag="O0286_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170534.1"
                     /translation="MISIHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHIKDAESKT
                     LHIIHPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            complement(6208..6384)
                     /locus_tag="O0286_002172"
     CDS             complement(6208..6384)
                     /locus_tag="O0286_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170535.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(6400..7152)
                     /locus_tag="O0286_002173"
     CDS             complement(6400..7152)
                     /locus_tag="O0286_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCY0170536.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            7203..7532
                     /locus_tag="O0286_002174"
     CDS             7203..7532
                     /locus_tag="O0286_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170537.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            complement(7521..7736)
                     /locus_tag="O0286_002175"
     CDS             complement(7521..7736)
                     /locus_tag="O0286_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCY0170538.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(7752..8015)
                     /locus_tag="O0286_002176"
     CDS             complement(7752..8015)
                     /locus_tag="O0286_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0170539.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            complement(8012..8186)
                     /locus_tag="O0286_002177"
                     /pseudo
     CDS             complement(8012..8186)
                     /locus_tag="O0286_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8149..8862
                     /locus_tag="O0286_002178"
     CDS             8149..8862
                     /locus_tag="O0286_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0170540.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            8878..>9371
                     /locus_tag="O0286_002179"
     CDS             8878..>9371
                     /locus_tag="O0286_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0170541.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLA"
BASE COUNT         2737 a         1746 c         1344 g         3544 t
ORIGIN      
        1 gattattaaa taaatagtcc gccaaattct ctttttcttt atcaatctga ttgtaattaa
       61 cactttcgac ttctgtagga attctaatgt caacagaagc attgatataa gcttgatgtt
      121 gcattcaatc acactcctaa tccttcatat aaaacggaga agtaaatccg tcactattca
      181 aattcaatcc ttttgcccaa tcgacaggct tattcatgat agtttcgatt tccttaagtc
      241 catttgaacc tctaggtatt tctacaatta cttcatcatg gacatgtcca actattttaa
      301 aacctgatgc ttcaagccta gctatagaaa tcgcaagtaa atcccttgca gttgcttgaa
      361 caatattctc gactaacttc ccaccatacg tttttaactt tgaccattta cggttaagat
      421 ctaagcccat aaattcaaca acttgactac cccaactatt ttcaccaact gaagcttttg
      481 gataagctaa agctcttcca ctaggcagtt caatcattag aaaacctttt ttcatataaa
      541 atctaactcc atgcgtatga tgcgtctttc gggatttcac agtattaatt gcagcctctt
      601 ggcaagcctt ccaaaaatta actatgttag gatttgcgtt acgccaacta tcaactaaac
      661 cttgtaattc attttcttca atgcccattt ccaatgcgcc catcgctttt aaagctccag
      721 cgccaccttg ataacctaaa gctaattcgg acacttttcc cttttgtctg agagggtcgc
      781 ctttagttat gctttctacc gggacattaa acatttgaga agccgatgct tcatatatct
      841 ttccgtgtgt gttgaacaca tctaaacgcc attgttcttt tgcataccat gctatgactc
      901 ttgcctctat tgcagaaaaa tcacttactg ctagttcatt accttcttca gcagtaaatg
      961 tcgtcctaac taattgactt aataagtctt gaggatgaac attgagtaat aaatctaaat
     1021 cgtcaaaacg ttgttcttta ataagatctc ttgctatttc taattcagta tctgaaatat
     1081 aatgctttgt taaattctga agttgtacac ctctacctgc ccatcttcca gtaccggcac
     1141 cgtaaaattg aaacagacct cttacccgtt catcactgca catcatgtca tgcattttgt
     1201 tgtatttttt cacactggtt ttagacattt gcaatctaat ttctagcatt tttttagctt
     1261 ttcctgttgc ttcttttaag tactcctgaa ccgttttctt ttgtaaatta ggtatatcta
     1321 atccttggtc atcctttaac caagccaata attgtgtagg actattagga ttttctaaac
     1381 ctgttatatg tttagcttga ttaagcaatt cttctttact ctgcttatcg agcacattag
     1441 ctcctaacat caatgattta gaaagcttaa tacctctgtc gtttatatgt tggtcaaaaa
     1501 cccaatatgc ttgttcaatt gcagttactg gaaagtcttt aattttatga gcaatcgtca
     1561 tttctacttc tacatctcga atacagtaat ctataaattg ttgccatttt tcaagatcat
     1621 gttcaggcaa gtttcttgtt cttcctccat taacttttgt tggtttacaa ggtatagaga
     1681 aataacgaat taaattttta cctgctttat ctttttggtt ttgtagtctt aaaacttctc
     1741 caactttatc aagcgaagca ggtaagccaa tacgcattga attaaccatt gtgcaaatcc
     1801 attcttcagg tggcatctgt ttattaaaat gtttagcaag acaagttctt tcgaaattag
     1861 cattgaatgc atactttttt acagcaggat caaaaagagc aattttaaac gtctcaaaat
     1921 cagcgtggaa aggctcatta tctactttag tcatgtcaat cgcactaatc gctccaccat
     1981 ctattgaata agctataatt aaaatttcga aatcttcagc ttctgtgtat ttataggcac
     2041 cacatttcga aatatcatta ctgctatatg tttcaatatc tatattcata aatctcaaat
     2101 tcttgacacc tcaatttctt taaaattaaa gtggggctaa aaccccacct attgacttat
     2161 aagaaatcct catcatcagt gtctaattca tcgaaatcat cttctgctgc acttgcaccg
     2221 ccaagaggtt cgcctttttc tacaagttga atgttgttca atccaactgc gataccctta
     2281 ttaccatttg tgttgaaagg aaatagatta attgaagctc taatataatc accacttaca
     2341 acagttccag aatccgttaa tctaattttg ttttggtcaa taataccagg tgcttgtttg
     2401 cttgatgcgt taataaaata agcgtcttga taattgacat catcttctct ttcagtatct
     2461 ccatcacgta atggaagttt cagatttgca ggaactttgc ctccaaactt actaactttt
     2521 ccttcttctt tagcagcttc tatagcttgt tcaatggctt ttatcgtact tgtatctgat
     2581 ttaggaatga ttaaactgat tgaatacttt gattcttgcc cttcttgcat actgtgaggt
     2641 tcaaaaatat gtgcatatga tgctcttact tttcctgtaa tcactttagt tttatttaat
     2701 acttttgctt tcatgtttat ataccgtcct tttaaatttt tatagtttgt caaaatcatc
     2761 ttcagcagat tgctttatag ctggtcgttt atccgactcg gtagcaagtg ttaatttacc
     2821 ttgcggcttt tctataaaac cctctgtaat tttagaaaat gcttttttac caattaattt
     2881 ttctaaattc gtaatgctaa gtaacttggt ttctgtaata tcttcaggtt tataacccgc
     2941 ttcaactaac ttttcaagca ttgcttttgt atcagttatc attcttcgtg aacgaccttc
     3001 tacaagcttc caaccaggat agtttttatc attttctttc gcttgattta gtgcatattg
     3061 ttctacttca tcagcccatt ttttgatatc aggcagttta tataaaagtt ctgcaatctc
     3121 ttcatcactt aacaaatgtg gtggcttttg aggcacattt tgcatgtatt ctgcacgtgt
     3181 tctacatgaa tgatttatct tacagaatct acaatgacta cctgctttaa actctcctcc
     3241 accgttataa gcaagtctgg ctaatggttt aacaaaatcg gctccccatt gaagtaatct
     3301 tgatattggt aactcttcag tagaaaagtt atctattcga ggttgtatga tagtcatgcg
     3361 aattgtatga atgtcataca ttaaactaag cagttcatat gcgcccaagc catataatct
     3421 aagttgagga ttatctatag ctgaaacttc aatgccttta ccatatttaa ggtcaataat
     3481 ttcaagtaca ccacctgaaa atataatgac atcaccagtt ccaaaagatt cagggacgta
     3541 tttacctaaa tctaattttg tttcaaataa agctattaca tcattatccc tactcaaagc
     3601 ttcgttatat ttttcttcta cattagctac atactcttcc acatattcac gcaattcttc
     3661 gctgtaatat tgatttctct tataattttg aaaagcctta ttaaactcaa actgtgttag
     3721 gccttcatat ttaaggctga aatataactc acttaactca tgggcgaatg taccttcttc
     3781 ggcaaaaact gtacttttat ctgcaatacc ttcacttgcc ttaatactcg gtgggcagtt
     3841 tagccattgt tttgctccac ttgcactaag ctttgcatga gctctatttg agtgatctag
     3901 cttcatgcat ttattctcgc attcataaaa tcaacaattt tttcataatg ctcttctttg
     3961 atagtagata gcttatccgc accaagttcg ttaagtttat ttctaaattc tttcttatca
     4021 gaagtatctg cttttttaag gaactctttt cctactgata aaacataatc tttagttaaa
     4081 tcagcagaag tttccttaac ttcttcaatt gattccagtt gagctgtttc ttcttttggc
     4141 attggtgctt ctttaacttt ctcttgtaca attgatgaat ctactgttga tagttcagtg
     4201 tttaacacac gtaaattgtt atttaatagt tttaattctt caaaaatatc ttctaatatt
     4261 gccattgatt aaatcctcct accatttcat gactaagtta attagtctgt cctgttcatc
     4321 tgtgttctct tcaatccatt cgtttataac gtcacgcatt gcatccgtcg caaaatatag
     4381 ttcgcttaaa tctacaacat gaaatgattt aagtggaata ttattcatat ccttgatttg
     4441 tatactgata ccgtcatgtt ttttcatcgc agacacttta aattcaaacc cgttaaagct
     4501 gataatttta ttttttatct caccaaattt gtaatacatt gttttagccc tccttgttat
     4561 catcaatacc gtgaaatttt tgtgatttac acatttggag aacattgaca atgtctttat
     4621 aactcttagt gctatccaat aaggaagcaa gatcgaaagt atgaccaatc acagaacttg
     4681 aacctgctaa ataatctccg tcgataactc ctattgatga gaaaagcaaa atatcaaatt
     4741 tactttctcc cttaatttct ttcgctaatt catacaattc tccgcttttt tcagataata
     4801 agtcttttac ttcttcctga gtcatgtctt tatatttttt agtcattgtt gacttcctcc
     4861 tcgttttctt cgtcctcctc gttatcttct tcgttttgta attcataaat tttgtttttt
     4921 agttttatat tttctttttc caatttttcg ttttttcttt cttccgcaaa atacttacct
     4981 ctgtaagtat cttcttcttt atctttaaca gcctttattt caataagttt tctgtactcg
     5041 ttcaatgtga ttgttactgt caattcttga tttgctacaa agttatcttc ttcatttctg
     5101 tatcctgaga aatctttagt gtaataatgt tgttcagttt taatattttc agccatagtt
     5161 gactacctcc gtatattttg atttaattaa gttgtatatt ttgataaatg tttgtcactg
     5221 ttacttgttg acgcaagtag cagttttttt attcttcata aaagtattct ttgtagtata
     5281 tgaatgttgc gatacttgcg aatcccgcaa ttgaccatgc tgtagtgaag tacagcaatg
     5341 gcataagcac aatcgctaag actgtgaagc acagtactgc tattaattag cttttatatg
     5401 tgtcgctcat ttaatatcct cctaatacca ttttttatgc tttctgatca aatactcttc
     5461 caatttagaa atattaatca gagtgcctgt tggtgaataa tcaatgtata aattttctac
     5521 acctaaatta tctttgcggt aatatttcaa ccagttgtat actgtacttc tacatactcc
     5581 aaacaattga tggatttgtg taggtgttgc gtataacttt ttcacaaatt tttcttcgcc
     5641 tctatatgtg ttttctggtg ttggtggtac tatgattttt ggcatctcta tcactccttt
     5701 cgataaatgt taaagtttgt tattattcgc tctgtattga agttctctat ctaatgcata
     5761 gaaaactttg tttatttcta agtagctgta atcacctttt ttaatacttc ctaatatttc
     5821 ctttcttagt cgacgttcat tttctgttaa agattctact gacgcatgat ctcttctgaa
     5881 aatccttggt atgattatgt ctaacccttc tgattttttg ttcatttgtt gttccacctt
     5941 tcgtgtataa tgttgttatc aacctaagga ggtgataagt atacataaga gattgctcac
     6001 tcaatattta gataaagaaa tcgttacttc tttagattta catttaatta atggtgaagt
     6061 tattaaagta caagaacata taaaagatgc tgaaagcaaa actctacaca tcattcatcc
     6121 aaaagataga gttgtcagtt tagatcatgt tttgtatttc gacattaacg ttaaaggtga
     6181 aaagaataac gatagtcctt atccatctta aaatccatag tgcttatagt tatttgctat
     6241 ttgttgacag ttatcactgg caagtcctac cttgtcagct tggtaactgt attgattatt
     6301 tacttcattt actaatcgat tccattcgtc tcttggtata tcttcaagaa gtaagagaat
     6361 catctttaat ttttcttcgt tcatttgtta ttcctccttt taagatgttt gtttttctcc
     6421 taaaaactta ttaacaaagt attgttgtcc tttgcctgtt acttttggcg tcttactaat
     6481 tgatgtgtga ccgtccgaat gtgtgattga tgtttcttta atttcgaata actcacgttc
     6541 cattgaatac tgtgtaggca tgttataatc cacacccttg cgtttaataa ggaatccgtt
     6601 ttgacgtaac cactcaaaca atctgcgttg cccgatgttt acaccgtttt gtttaatgat
     6661 ctttgctaac tctccaacta aaattgatgt cttagtagta gctactgcat ctgcaaatac
     6721 aatttttggt ttatcacgtt caatctttgt ttctaattga ttgattgtgt tgttagcaat
     6781 ttttaaagca cgttgcataa tcatttctgg gctgttccat gctttttcaa cttggatgaa
     6841 gtattgtctt gcacgtttac caggttcact acgttgaatc attgcgattt cttttgcagt
     6901 gtctagtgtg agtgcgtggt caatatagtg tgtcatattg ccttgagctg ttgctctttt
     6961 ttgagcgata gctgtgtaat ctgtattttc ttcaaatccg tatttaagca ttcttggaaa
     7021 ccaatcttta tatgctgtct taacttctaa tgcttgatga agttctcgac cgctgattgc
     7081 gatttctcca ttttcttttt cttgaatatt gaacatttct ccgatgttcg aatttgtttg
     7141 taatgcttgc atattgttta tgctcctttc tgctatactc ctattaagga ggtgaatgac
     7201 ttatgactaa tgaagctaaa tttgtccttt tacaacttta ttcaatttac cttgatagaa
     7261 ttgacgaagg tatgtctaaa cgttctgcat cttatttcgg tagtgatgaa tcctcattta
     7321 acgctttctt tttagatttt aattttgaag actatatcga tgcagttctt gaattaaagc
     7381 atagagattt tgtaattgct tctgctgaag atggcggttt tcttgagatg gctctttctc
     7441 gagaaggtat cgcctactca gaatcagaat ccaaaaaaga ttacaaaaca cttatggatt
     7501 taattagaga tttgaaaaaa ttaataatct aaaatccaat catctgctat taaatcgtct
     7561 gcgctaggct gccaccttcc ggcggcggtt tgtctttttt tcttatagtg tctagatacg
     7621 actaggcatt gataacgctg caaattagtt ggtaatatcc catacacatc ttgattctct
     7681 ctccttatac ttattccttt ctccatcgct agcttcgttg cttcttgaat gttcatttgt
     7741 tattcctcct attaagatgt ttgtttttct ttaaatgcta aaataattga tttcttttta
     7801 tcattcgtaa atacgaaatt ttcgtattca ttacctaaaa aaatatcatc atatttaaca
     7861 ttaaaagcac tcatatactt agaaagtaaa ctatctttaa tgtttgtaga gtctttttcc
     7921 atattttgaa ttgtacgtga tgagacctta aataaatctc ctaactcttt ttgagtcaat
     7981 ccgtaatcag tcctcaactc ttttaatgtt ttcatgttgt caccgccttt cgtaaaccta
     8041 atataatacg aaattttcgt attgtcaaca ttaaatacgt ttttttcgta aaaaaacttt
     8101 actatgatat gaaaatttcg tataataaga aaaaaggagg taagtaatat gaacaaagaa
     8161 agaaatatta ttatagccaa aaacattaga aaatttctca acgattcaaa tatgtctcaa
     8221 aagaaacttg ctgaactcat taacataaaa ccatctactt taagcgatta tttaaattta
     8281 cgttccaacc cctctcacgg cgttatacaa aggatagctg atgttttcga ggttggtaaa
     8341 agcgacatag atactacata caaagacgat aacgacatca cttccatata caacaaactc
     8401 acacctcccc gccaagaaaa cgtacttaac tatgcaaatg aacaattgga agaacagaat
     8461 tctaaaggag ataacgttgt agatattaat tcatataaac aggagaaaac tccagttaac
     8521 gtcaatggtt gcgtctctgc tggtgtagga gaacgtttac acgatgaaac gctatttact
     8581 gaaatggtta aaggacctat ccccacacac gatttagcgt taaaagtaaa tggtgattct
     8641 atggaaccta tgtttaaaga tggcgaaatc atatttgtgg agaaaactca caatataaag
     8701 aatggacaaa ttggtatatt catcattgaa gaagaagcgt acgttaagaa agtctttgtt
     8761 gaagatgata gattgactct agtttcacta aataaagatt acgacgatct acacttttat
     8821 agaaatgaaa gtgtgaggtt aattggaaaa gttattttat aaaaggagca cttgcaaatg
     8881 aaaaaatatg atattgcagt cttagacttt gaaactatga atgaacatat gaacagccct
     8941 tgcgaagttg ctgtatcttt aattaaggat ttatcaatag taaaagttta ttcatcttat
     9001 attaatcctc ctaataatag atataacttg aaaaacgcta aaatacataa aatacctgaa
     9061 gatgtcatat taaaagcacc taaatatcca gatatttacc aagaaattct ctatctttta
     9121 aaagaatcac atttaattat tgctcataat gcactttttg atatttcagt attaaaaaat
     9181 actaataatt attatgactt acctgttcca aacttcatgt atgtcgatag tataaatatc
     9241 tttagaagct tccacgcaat ctctagtttt aaattagaaa atttgtgtag cttatatgat
     9301 atcgataaag aaaaattaca ttctgctaaa tttgacgtgc tagctttatc gaagatgttg
     9361 atatcactcg c
//
LOCUS       DAKTGS010000114         9367 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.114, whole genome shotgun sequence.
ACCESSION   DAKTGS010000114 DAKTGS010000000
VERSION     DAKTGS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.114"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(215..640)
                     /locus_tag="O0286_002180"
     CDS             complement(215..640)
                     /locus_tag="O0286_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0170542.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            934..1749
                     /locus_tag="O0286_002181"
     CDS             934..1749
                     /locus_tag="O0286_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCY0170543.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            1960..2814
                     /locus_tag="O0286_002182"
     CDS             1960..2814
                     /locus_tag="O0286_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0170544.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(2874..5297)
                     /gene="hysA"
                     /locus_tag="O0286_002183"
     CDS             complement(2874..5297)
                     /gene="hysA"
                     /locus_tag="O0286_002183"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HCY0170545.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDNYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(5294..5461)
                     /locus_tag="O0286_002184"
     CDS             complement(5294..5461)
                     /locus_tag="O0286_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170546.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            5705..6121
                     /locus_tag="O0286_002185"
     CDS             5705..6121
                     /locus_tag="O0286_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0170547.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            6191..7039
                     /locus_tag="O0286_002186"
     CDS             6191..7039
                     /locus_tag="O0286_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0170548.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            7259..7384
                     /locus_tag="O0286_002187"
     CDS             7259..7384
                     /locus_tag="O0286_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170549.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            7646..8253
                     /locus_tag="O0286_002188"
                     /pseudo
     CDS             7646..8253
                     /locus_tag="O0286_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
     gene            complement(8346..8507)
                     /locus_tag="O0286_002189"
     CDS             complement(8346..8507)
                     /locus_tag="O0286_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170550.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(8544..9284)
                     /locus_tag="O0286_002190"
     CDS             complement(8544..9284)
                     /locus_tag="O0286_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCY0170551.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
BASE COUNT         3274 a         1367 c         1505 g         3221 t
ORIGIN      
        1 ttagcagtag tgaggggatt tattaggttc cagatatttg agatttgctg ttatgtttag
       61 attataaatt gtggtacaca ctcatataaa atttactatt gtataggcca atctgttact
      121 acgagaagca aacaacaata atttacaagt tcaataacta aaaagacaaa cgccaatttt
      181 tcagcgcttg ccctataaaa ctattttcaa attattattt aactacaatt tgtatctgtt
      241 tgattgtact tggatcaatt gaatttttag cgtcgtcatt tttctttaga ttcactactt
      301 ttttattacc atcattcaaa gtaagcgtat aagttgctgt ttgggcatta ttaatttttt
      361 ctgttgtaac accacgttga gaagctaatt cattttttac tttactgtca atttcttgat
      421 aaagaacatt tttattttct gggaagataa agtaagttcg atgtaatgca gtaataccat
      481 ctactgaaat tgtgtaagga acagtgtgat aaccatccac agtcatttct ttatagccgt
      541 tattactatc tgcagatgct tcgtgactcg gtaatgcagc tccgaccgtt gataataatc
      601 ccaatgctaa agtggcagta acaaatgatt ttagtttcat atctcaatct ccttttcggg
      661 ttgtatgata ttacactatt tgtataacac gtttcataaa gtttaaaaca tactttttgc
      721 attttttata ttttaatttt tagtaatagt ttttaacgag ggatatgact cgtgcatttt
      781 gaaaagtggt aagcaatgtt tttagtttat agggattgat tagttgtata taataatcat
      841 gaaaagcata tagtcgatct tatactttta attgagactt atagtgtatt taagttatag
      901 ataaattcat taaaatatta tgggggaatg agtatgtcaa agcgtctatt attgtttgat
      961 tttgatgaaa catattttaa acataataca aatgaagaag atttaagtca tttaagagaa
     1021 atggaaaaat tgttggaaaa actaactaat aataatgaag tgatcactgc tgtattgaca
     1081 ggaagtacat ttcaaagtgt catggataaa atggatcaag ttaatatgac gtttaaacca
     1141 ttacatatct tttctgattt gagttctaaa atgttcactt ggaataatgg cgaatatgtt
     1201 gaatcagaaa catataaaaa gaaagtcttg agcgaaccat ttttatttga agatattgaa
     1261 gatatattac gtcatatttc tgcgcaatat aatgtcgaat ttattccaca aagagcattt
     1321 gaaggtaatg aaacacatta taatttttat tttcattcaa caggtaatca caacaatgat
     1381 agtcgtatcc tagaagcact tgtcagatac gcaaatgacc aaaactatac agcgagattt
     1441 agtcgaagca atccattagc tggtgatcct gaaaatgcgt atgatattga cttcacaccg
     1501 tcaaatgctg gaaaattata tgctactcaa tttctgatga ggaaatataa tataccagta
     1561 aaatcaatat taggttttgg agatagcggc aatgacgaag catatttaag ctatcttgaa
     1621 catgcatatt tgatgtcgaa tagtagagat gaagcattaa agcagaaatt tagattaaca
     1681 aaatatccat attatcaagg gataacactg catgttaaag aatttgttga aggtaagtat
     1741 gattattgat tagttgagtg atatgtatac tttttaagtg caaatattaa tatttaaggg
     1801 atgattagaa aatatggtga atcgactaca ctttagcttt aaaaaattat ttatatgttg
     1861 atttatccaa ttaagtgtag acgattcagg tagcgcaaaa atgacgcagg aactggagaa
     1921 agaattgata ccaaaattaa tataataggt taagtatata tgaataaaac aaactttggg
     1981 gcttatttaa ataaaagtaa gagcaggaaa atatataaga tttttgatca aaatttgaag
     2041 cgagagttta gttattggca gtttaggaaa cttttcaaat cttatacaag gcagtctgat
     2101 gtacatgaat taaaagtgtg tactcaaaat gatggtacta ttgagcaaat atggatagat
     2161 gaagcaaatc aatttggagt gtcagtgact tggaaagata atagtattat cggatttgtt
     2221 atgcgtccgt taattaatta tgatgcgatt aagaatgaaa cgactttaaa atacgcagtg
     2281 ccgacaacag attgctatga gattttttgg ggtggagata atgaattata taattatcac
     2341 tatttatacc cacatcaaag gtatgctatt gatttagtta ttaaagaaaa tcataaaaca
     2401 tataagaatc aaggaaaagt aaatactgat tatttttgtt ttggaaaaga tatcattgcg
     2461 ccagcaaatg gtacagttga aaaagtagtg aatggggttc aagacaacag tattggaagt
     2521 acgaatgaat cacagttttt aggaaattat attgtaatta agcacgcaga aaatgagtat
     2581 agcttaatag ctcatttaca ccaatattca atcattgtga atgaggggca aaatgttaaa
     2641 tatggtgata tcattgggaa ggttgggaat tctggcaatt ctactgaacc tcatatacat
     2701 tttcaagtaa tgaatgataa gaatattgaa gcatgtacat ctttaaaaat tcgatttata
     2761 aataatcgag aacttatcaa aggggatgtg gtctgcggat tacaagctga atgatggcga
     2821 tacttataaa atctcgacac tataaaaatg gtatagtgtc gagattttct tgcttattta
     2881 gttaattcaa agtgcacgcc ggattcatta gaagtcgacg tatttttgtt tgtaatagag
     2941 taaccggtca ttgaaatttt agattcaata tctgaagcgg aatttgtaga ttcaggatta
     3001 tagaagctac attcataagt gttatcatct ttctttttaa gtataaacat acctttggct
     3061 ttaacttcga ctttagtgtt gttaatgtca aaagtttgag tgctattact ataattaaca
     3121 ccagcccaaa ccgattcatt atctttcaca acgtggaagt catcttcttg cttaacgaca
     3181 gttacttcat ctttttttgt cttaaagaca tctttagata agcctggata caacacatat
     3241 ccatatttat tgtcagaatt agaatgcttt tgagttactt cataatactc atcagttttt
     3301 tgtgtatcct tttgactttt atttatttct ttccacttac cagtatgact ttcttttttt
     3361 acagttattt tcggtttgtt taaaaaatga taaccgatgt tctttttggt atctgtggac
     3421 tctaaaaaga ctgaattgtt ttcctgatta tcagaattgg ttgtttgttt atcgtctgta
     3481 tataacgtat acccattcgc tttgcgattt tcaatcgttg taactggatt ctttgatgaa
     3541 tcagtacttt taatgccagt tcctaagaag acaattttat cgtttaatat gaaatatgat
     3601 tttttggcag ttaaagtttt gtcctgattt tcaaaatcca ttccgatact agcatgttgg
     3661 tcatcaactt ttgttccgcc aacaaaagtt ttactcgact ttttatcatc cgtatctttt
     3721 aatatttcat tgtctaaagt tgttgtacct gataaacgtt tcatatcggc tgtcacccag
     3781 aagttatcat gatagtgttt gacatcgctg ttatataaat aagacattcc agcaccagtg
     3841 tgccaacctt ttaaattctc tccgttgata ctttcatagc gtgctacgtt tttcgacgtc
     3901 atacttaaac caaatgcaaa gtctaagtct ttgttatgat aggtgacacg atccatgtca
     3961 ttatatattt taagttgttg tgttaatccg tttttagaaa tactgttatc tgtcattaaa
     4021 gacttcattt tatctatgtc cgaatatgaa tttaaataat cattttgttt ataacttgaa
     4081 tctgactcta ctgaagattt gacaatcttt ttatacttag cttttgttga atcatccatg
     4141 gcatcactta atctcaacaa tgatttcatt actgttgcag atgctgagtg actggtttca
     4201 ttttcacgac tgatagctct acctcgtgat aaatccatca tttcaccttt ataaatgagt
     4261 ggcataaatc cgtcgtcaat ccatgacttt aaggttgtat cattttgggt tttatcatta
     4321 aaaggtgttt cttttatcat cggcatcatt tgagaaatcc cctctaagag tacaacgcca
     4381 taagcaccag tgtatggaac gtcttgatga tcaatgtaag agccatcttt ataaaatcca
     4441 ttacgttctt taccagtggc agaatcttga acgtaagtga agactttatt aaatgaatct
     4501 atagactttt tcatcatatc tttatcttct tcgataatac attctaaaag tttcacctta
     4561 gaaatgtcta ctagatttcc gcctttagca agttcagctt ttcctacaga agataatatt
     4621 ttgtcactat ctggggcgaa agttttaata ggggcagtga attttttctt ttcttcattt
     4681 gaaaattgat cattcagcaa tataagcgta tttgttaatg atttaggtgt tccaatttca
     4741 taatcccacc aatttaagtt ggtattcttg ccagttgttt tagtaaaatt ctcacttaat
     4801 tcttttactt ttttatctgg ttcttttcca tatgcatttt tatgcagcca ctcaagggca
     4861 tctttcactt tcttcttatt ttcgtcagta tttaaagtgg ttttaggatt cctcatcgct
     4921 tctgcgattt tctcaatatt acgataggta cgagtcatat gagaagaatt agtttcaagg
     4981 gtttccgctc ctgaccacaa gtatttccta ccactttcag ttttcatttc cttgagtaaa
     5041 ttcgtcgcct ctttctctgt agcatcaaac ttcttcttca tatctggatt attctcatca
     5101 taattatcat aaccatagtt aacgtccagc catgtgttcc tcaatttttc ataatctggc
     5161 gtttgaacat tcgtatcagc cacagcgatt tgatgtttat caacacttct gaattcacca
     5221 ccattcaaag taatcacacc agccattaat aacgtaatgg tggataattt ttgccatttc
     5281 tttattctat atgtcattga catgtctcct ttttgtgttg cgcgtgcgca atgaatatta
     5341 tgattaaata atgattcaat ttttcaaaat tcgttaacgt atacaaatga ctgtctactg
     5401 tcaaacaatc cacaaagaat gttgatgcat ataaacaatc gatcacccaa attttccgca
     5461 tcacccaaaa attgcaacta aaataatttt caaaaaatta aatattatca atcttagtga
     5521 ttatatcatt ttaaatattt aagttataca atttttaaag taattaaaaa cgatgaaatg
     5581 taaaatgtta aaaaagagac aaatttaaat ttgagtaata aaataagaag tattcaaacg
     5641 aagatgacaa tatataaagc aacattactt tagacgacca aacataactg aaaggatagt
     5701 cgcaatgaaa actaaagaag tcgtagcgct catgaatata tctcaagaca ctttaagata
     5761 ttatgaaaag gttggtgtga ttccaccagt taatcgagat gaaaatggat atagaatata
     5821 taatgatagt gatttaaatt ggatttattt agtgaaaaat ttgcgaaatg caggcgtcag
     5881 tattgaatcg cttatcgaat tttgcaggtt agcgcagctt cctaaaaatg aaaatattca
     5941 agcacagcaa aagcaaattt taaataagca actcgaagaa ttaaatgaaa acttaaagac
     6001 aattcatgat gtgagagatt tactacaata taaaattgat aattatgata atcatattgc
     6061 taaaatcaat gctagtgata attatgatga caatgttgaa cgtctttggg agagaaagta
     6121 aacctatggc ttgctataga gttcgctcca tggtttatag tggacataca agataaagga
     6181 ggttcatttt atgaatcata ttgaaataag taaagatgta aagataccag tattaggttt
     6241 cggagtattt caaattcctc aagaacaaac agcagaagcg gttaaagaag caattaaagc
     6301 gggttataga catattgata cagcacaaag ttatttaaat gaaactgaag taggtcaagg
     6361 tattgaagct tcaggtattg atagaagtga actattcatt acgactaaag tatggattga
     6421 aaatgttaac tatgaagata ctatcaaatc aatcgaacga tcattacaac gattaaattt
     6481 ggactatctt gatctagtgc ttattcacca accttacaat gacgtatatg gctcatggcg
     6541 tgcattagaa gagttaaaag aaaatggtaa aattaaagca atcggtgtat ctaacttcgg
     6601 cgttgatcga attgtagatt taggtattca caatcaaatt caaccacaag tgaaccaaat
     6661 agaaattaat ccattccatc aacaagaaga acaagttgca gcacttcaac aagaaaatgt
     6721 tgtggtagaa gcttgggcac catttgcaga aggtaaaaat caattattcc aaaatcaatt
     6781 gttgcaggcc attgcagata aatacaacaa atcaattgca caagttattt tacgctggtt
     6841 agtagaacgt gacattgttg tactcgcaaa atcagtgaat ccagaacgta tggctcaaaa
     6901 cttagatata tttgattttg aacttacaga agaagataaa caacaaattg cgacattaga
     6961 agaaagtaat agccaatttt tctcacatgc tgatccagag atgattaaag cacttacaag
     7021 cagagaatta gatgtttaat cataatataa caaagtaaat catttacatt acactaacct
     7081 gaaacttgga tgttaaagtc tatgtttcag gttatttttt actcttaaaa agtgaaagga
     7141 gcaagcaaat aagttattat atatttttat attatgtaat aacgtaacta aggttataat
     7201 tagataatta atatgtcttg gaattaatta tttaataagt tgtatagcag gtggcgatat
     7261 gagatatata cacttttcta ttattacatt aattacaggt atcattatgc atattacaat
     7321 gtactttgta ccatttgaat ctcggaaaat gccactattt atgacgataa tttttacaat
     7381 ctaaaagcag taaagactca taatataacc aaaaacatta ataaaagttt gttaaatgat
     7441 aaggggatga gtattggtga atttactcaa aaggtttaag ggaaaaatcc tacctggaga
     7501 gattcaaaaa ctaaatggac tatttccaaa aataaagggc aatcacatgg tggttcttat
     7561 tggaagttaa taaataacaa aggaaaaaga atagcttctt taactaaaga aggaaaaatc
     7621 ttaagggaat aaggtgttgt aaattatgtg ctttgatata aataatttgg atataaaaaa
     7681 gttgaatttt agaaaggtaa aaaacgcaat tcatttaagt ttaagtggcg ataaatacca
     7741 gtttttggag gacggtaaaa ctatcaataa tacttatttt tagctgtata tgataatgca
     7801 ataaacattt ttgaagattt gttttcgcct tctgattcta taaatttggt tcatgtagta
     7861 tatgtttata gtgaacctta taagaaaaca aatgttataa aaaaatttac atcgttaaca
     7921 atagatgaat tgtattactt taaagaatat aaaaaggaga aggatgataa tattttgtgc
     7981 gaggagtttt catataagtg ttccataaaa gatataaaat ataaaaaatt attaaaagct
     8041 atatccaatc aagactttaa agggttagtt ccaaatatca atcagaatga taattattct
     8101 gaaatatata taattaacaa tactaaaaac ttaatatatc atttatatga tgatagaggt
     8161 ctatggctag cttttaataa taatgaagat tatgcaagat attctgaaaa atataatgat
     8221 ttaatatcgg aatttgataa tgaagatatc taatttttta atattcgtag cttttatttt
     8281 tctacttatt ttatgtttat ttttaatctt acaaatgaca aaccattaaa agtagcatcc
     8341 caacatcaaa caaaaaaaca attcatcaaa taaaaatcgc tacaaaacca agtcattaaa
     8401 cacgcaataa ttaaaatttt ccactcatta taattctgaa ttccaaatgt cgaattccga
     8461 aaaccaaact ccaaattcca aaaacgcaac tccaaaatta aaagcatttc cctaccattc
     8521 gggaaatgct ttttacatac tgattactct gtcattaatg attttacaac ggaaaccatg
     8581 tcgtcatgta tgaccaaagt agcgtcgcta tcataaggtg ttcgatcttt attgataata
     8641 attaaattgt cgcctttaaa atgtgatatt aatcctgcgg caggttgtac aacgagtgat
     8701 gaacctagta caacaagggt gtcagcatgt tcaattttat ttaatgccct tatgatggta
     8761 ggttgatcta acatttcacc gtataatacg atgtccggtc gaatggcacc accacaatta
     8821 tcacaatgtt tcaaagttct atcaataaca tctgacttcg tataagattt atgacataca
     8881 ttacaataaa aacgatttaa cgtgccatgt aattcatcaa catgttgact tccagcgtct
     8941 gagtgcaaac catcgatatt ttgcgtgatg acacctaaag attgttgatt acgttctaat
     9001 tttgcaatcc aatcatgaac gatattgggc atcgtatcga caaatagtaa gcgcttatgg
     9061 cagaaattga taaaaccttc aggatcatct tctaaataat cacggcttaa caagtattct
     9121 ggcgaaagcc catcttttga aatttcatca aataagccac ccattgaacg gaaatctgga
     9181 acgccacttg cgacagatac accagcacct gtaaaaaatg taatacgatt cgaactatct
     9241 ataatatgtt ttagtgtctc taaatcatgt ttcaagttaa acaccccatc aaaataatga
     9301 atgatttata acgtctttta cttttattat agtatattca ttttacgatt atcaaaagcg
     9361 cagttta
//
LOCUS       DAKTGS010000115         9195 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.115, whole genome shotgun sequence.
ACCESSION   DAKTGS010000115 DAKTGS010000000
VERSION     DAKTGS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.115"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..220
                     /locus_tag="O0286_002191"
     CDS             <1..220
                     /locus_tag="O0286_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170552.1"
                     /translation="TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYI
                     ILILSLLIFVTLLLVNKTGKIKYAHKEE"
     gene            complement(292..588)
                     /locus_tag="O0286_002192"
     CDS             complement(292..588)
                     /locus_tag="O0286_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCY0170553.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(637..840)
                     /locus_tag="O0286_002193"
     CDS             complement(637..840)
                     /locus_tag="O0286_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170554.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            1149..2135
                     /locus_tag="O0286_002194"
     CDS             1149..2135
                     /locus_tag="O0286_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCY0170555.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            complement(2802..3995)
                     /gene="sqr"
                     /locus_tag="O0286_002195"
                     /pseudo
     CDS             complement(2802..3995)
                     /gene="sqr"
                     /locus_tag="O0286_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(4014..5348)
                     /gene="cstB"
                     /locus_tag="O0286_002196"
     CDS             complement(4014..5348)
                     /gene="cstB"
                     /locus_tag="O0286_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCY0170556.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(5379..6446)
                     /gene="cstA"
                     /locus_tag="O0286_002197"
     CDS             complement(5379..6446)
                     /gene="cstA"
                     /locus_tag="O0286_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCY0170557.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            6583..6843
                     /gene="cstR"
                     /locus_tag="O0286_002198"
     CDS             6583..6843
                     /gene="cstR"
                     /locus_tag="O0286_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCY0170558.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            6843..7598
                     /locus_tag="O0286_002199"
     CDS             6843..7598
                     /locus_tag="O0286_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0170559.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGIFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            7696..7797
                     /locus_tag="O0286_002200"
     CDS             7696..7797
                     /locus_tag="O0286_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170560.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            7813..8265
                     /locus_tag="O0286_002201"
                     /pseudo
     CDS             7813..8265
                     /locus_tag="O0286_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(8359..>9195)
                     /locus_tag="O0286_002202"
     CDS             complement(8359..>9195)
                     /locus_tag="O0286_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCY0170561.1"
                     /translation="DPEVDYITEIGDYLLKYDVNSINDVRIEVIDNLGHSNNMLLKTI
                     LHAEVEMEDGKKYLLQNGKWGYFNREFFDLLNDHLNEIEIRYNTLTPTGLVFKEGEEG
                     YIKEIVGRLPEEYLMLHKKFIKPINKNFIVKGNGIELADLYNIKNKELFTIKRGINTS
                     LSLYSLEQNIIAINALKYPESYNFEELIEAIPDNSENIFNDIQRSTNFSIVWILPISS
                     IDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVEWSLYLKDQRINPIIYMETPTED
                     RN"
BASE COUNT         2991 a         1562 c         1357 g         3285 t
ORIGIN      
        1 cactgtttca gataaatatg tcggtcgctc atttacgata tggacgtcaa tttcattgtt
       61 actgcaaagt ttaattgctc catttttagg aagatggatt aatgaaatta atgataaatt
      121 cggtttctat attatactca ttttatcctt actcatattt gttacactgc tgcttgttaa
      181 caaaacaggc aaaataaaat atgcacataa agaagagtga ccgtcactct tctttaacaa
      241 gcgaccattt atcgatgggc ttagttctct ctgcacccac acttcactac ttcacttttt
      301 caaatcattt tttatggtct taaataaatc agtgagattt gttgcttcgg taaagtctag
      361 aattaatatc atttctttag aacctggata tggcgaaact aatgtattat cttgcaattt
      421 ctgctgggca cttttagtcg ccttgaccaa taatctatta tcatacaaac cacctataac
      481 cacgccatca taataaataa tatattctcc catcatcttt cttctcttaa ccgcgtttga
      541 attcacatga tttaaaaata aatcatgtac atctttctta gtcgccattg taatcgctcc
      601 ttcagtttta tgtttaatca cattcgtatt aatgattcat ttcgtgttgc tcttaatttt
      661 atctataatt atatgcgtag ttaaaatcaa acctatggaa aagaaaataa tgataacaat
      721 gttaagaaat atagttataa aattatagtt tggaaggtat gcgaatagca gaaaaatagg
      781 tatcgcaaaa aataaatccc accaacctaa actttttaaa gaatgcttta aaccttccat
      841 aatatcacct ttataaattt gtctttgtta taagataact aaaaaatcgc tttactgtaa
      901 aagtagccaa agaaaattct gaatcatatt cataagtagt gtatcattaa taatgaacaa
      961 tttaatacta taatccttga tctttgtatt gatcaactta ccacaacatt tattttagac
     1021 tactcttaga cttccctttc aaatggttgc atctattgaa attccttttg tataagttag
     1081 gcttttgtgg taatatcatc atgcataaaa aatcgagata ctaattataa agagggtata
     1141 aatatattat gaaagaaaat ttttggagtg aattaccacg tccatttttt attttggcgc
     1201 caatggaaga cgttacagat atcgtctttc gacacgttgt aagtgaagca gctagaccgg
     1261 atgtgttttt cactgaattt acaaatactg aaagcttttg ccaccctgaa ggcatacata
     1321 gtgtgcgcgg acgcttaact tttagtgaag atgaacagcc gatggtcgct catatatggg
     1381 gagataagcc agaacagttc cgtgaaacga gtattcaatt agctaaaatg ggctttaaag
     1441 gcatagactt aaatatggga tgtcctgtag caaatgttgc taaaaagggt aagggttccg
     1501 gcttaatctt aagacctgac gttgctgccg aaattattca agcgactaaa gcaggtgggc
     1561 ttccggtaag tgttaaaaca cgccttggct actatgaaat cgatgaatgg aaagattggt
     1621 tgaagcacgt cttcgaacaa gacattgcca atttatctat tcatcttcgt acacgtaaag
     1681 aaatgagtaa agtagatgca cattgggaat taatcgaagc tattaaaaat ttacgtgacg
     1741 aaattgcacc aaatacattg ttaacaatta acggtgatat tcccgataga aaaacaggac
     1801 ttgaactggc agaaaaatat ggcattgatg gcgtcatgat tggtagaggc attttccaca
     1861 atccattcgc ttttgaaaaa gaaccacgcg aacacacaag caaggaacta ttagatctat
     1921 tgagattgca tttatcattg tttaacaaat atgaaaaaga tgaaatacga caattcaaga
     1981 gcttgcgtag attctttaaa atctatgtgc gtggcataag aggcgctagc gaacttcgac
     2041 atcaattgat gaacacacaa tcaattgcag aagcacgagc actactcgat gaatttgaag
     2101 cccaaatgga cgaagacgtt aaaattgaat tatagtatga gtaataaagt ttatggatga
     2161 tatttcccaa tttaacacgg attgaacact ttctacttag gtattatctt ggttttcctg
     2221 ataggataac tcccgatgat gcctttttac ttatgtcact tatccttttg gaaatggtgc
     2281 aaaattaaaa atacatcagg atatcgttta actcagaaaa aaactccaat caaaaaaaat
     2341 gtttacaaca cattatgaaa ttaaaattgc tcttagtgtt caacagacaa agttattgaa
     2401 attactattg gaggtacata ccaagctatc attttgagta cacgtggttt gaatatctgg
     2461 caagaaatgg aaattggtaa tgtcagtcat aaagtcgcta aacgtgctca aataccatta
     2521 attattttta aataatttag aggatgaggt ataaaccttt aaaaaacagc agtgagatga
     2581 ttttcaatta gaaaatatct tactgctgtt tctattttca taatcattct tattgaatga
     2641 cttcatcgtg atcatttaaa acagtaacaa ctttttcaat cttatactat ttagagctaa
     2701 aaattcacga catataaaca caacactgac acgtcattta aatacttaat cttttatagt
     2761 aaaagatttt tcattaaaaa tttattgttt tctgtacttt attatattaa tccttttagc
     2821 atgccgtacc aatacatttt aggtaataaa tctttcttaa atatatacat acttctacgt
     2881 tctttggcct gattaaacgg cattgtttct ttagtatttt tattataatc aaactctgca
     2941 agtattaacc tattatatcc agtaacaata gggcatgaag tataaccatc ataatgatgc
     3001 gttaacattt gattattcat cacttgcaat aaattattag cgacgatagg tgcttgctta
     3061 cgaatagctg cgcctgtttt tgaagtaggt acatttgaag catcaccaag tgcaaataca
     3121 ttagagtagc ttttatgctg taatgtggtt gggttaacat ctacccaacc ctcactatct
     3181 gaaagtgtac tttcttttac tacatctaag ggacccatag gtggtgttac atgtaacata
     3241 tcataactta ttgtttttct atcgtatgct ttgatatgtt cgaatgtagc cacttttttg
     3301 tcaccgtcga tttcaacaag gttataatta taattgactg ttatatttct ttcttcaaca
     3361 atcctttcta attctttatt atattttcct acgtcaaata aagcatcttt tggcgttgca
     3421 tatatcacat tagcgttaga acggatttta tgtttcctaa aataatcttc agctaaatac
     3481 ataattttca taggcgcacc tccacacttt ataggagtgt ttggatgcgt aaaaatggca
     3541 tttccttgtt taaaattaga aatttggttc caagtttcgt taacatagtc aggtgaatag
     3601 ttagagcaaa caccattttt acctatattt tcttttagtc ctttaattga agaccaatta
     3661 atctgtaatc ctggagctac tactaaaaaa tcataataaa cgactgtatt atctcctaaa
     3721 ataacgctat tattttcagg ttaaaaactt gaaacagcct gttttatcca gttagcacct
     3781 tcaggtataa cactttccat atctttacga gaacttttca aactagatac ccctgcacca
     3841 accaacgtcc ataatggttg atagtaatga tggtctgctg gatctataat tgctattttc
     3901 tcttttaagt tttgattttt tcttaatagt cttgatgcta cggtaacacc tgctgtaccg
     3961 ccaccaataa taacaatttg ataatgctta ttcatttttt ccacatcctt aaattatttt
     4021 aatgattctg gaaaatcttg atagccttct ctaatattca ccacattttc aaaacctttg
     4081 ctttccaata tacccactgc aattgaactt ctaacacctg actgacaatg tacatatatt
     4141 ttatcctctt tattaaaagg aatattttca tttaataatt taccatgcgg aatattaact
     4201 gcttgatcta agtgtccatt attccactct tcatcattac gtacgtctaa tacatgttct
     4261 tctttacctg tcatatcagc gctatgaacg gactgggttg aaatgcctga ttttggcaaa
     4321 cgatagcctg ctaccttatc aaacccaatt aattgtaaag tgtgtttcgc tttctcaaca
     4381 gtagatttat ctccaattaa atctatatct ttttcaaaat ctaagtacca accaatttga
     4441 ttaataaagt ttttgttgta tggtatattg attgttcctt ttgtgtggcc accgtgaaag
     4501 gcctctttgc tacgaagatc aaatgctact ctcttattat ctaaactagg ataaacatca
     4561 tatgattgat ataaattcat accaaactga ttaacttgtt tcatttgtgc aaaatgatgc
     4621 ggtggtgctg gttgatttga tgttaatgat tcaataaatt tagtctcatc aatttcatta
     4681 aatgcccagt tattaatttt ctcataacct attgtagata taggtatggc acctaatgct
     4741 ttaccacaag ggcttccagc accatgaccc ggccagattt gaacatagtc tggtaaattt
     4801 ttaatatttt gaacggactc atacatttgt ttcgcgctaa tttctgtaga accctttatt
     4861 tgaacagatt tttctaataa atcaggtcta cctatatcac caacaaaaat aaagtcacca
     4921 ctaaataatc ccatcggaac acttgaacca ccacctaaat cagtgagtaa aaagctaata
     4981 ctttcaggcg tgtgtcctgg agtatgcaga acttctaatt taacattgcc tacttgaatg
     5041 atatctccat gtttaacaaa ttgtgttttt gatggcatat ttttataccc taatgcatct
     5101 tcaccttcgc cagacacata tatatttgca tttaagcgtt tagccacatc acgaattcct
     5161 gaagcaaaat cagcatgaat atgtgtttct gtagcttgtg taattgttaa accttcagaa
     5221 tctgcaactt ctatatattt cgataaatca cgaacagggt ctattattat tgcctctcct
     5281 gtacgttgac aacccactaa atatgatgct tgagataaat gattatcgta aaactgttta
     5341 aaaaacatga taacaactcc ttttattaat agatttaatt agataaataa attatgattc
     5401 gcattttcag tagcaccaat ataagtgcct acaccaccgt actcaacttc atctctaagt
     5461 tcttctttct gaatacccat gacatccata ctcatcgtac aagcgattaa tttgatattt
     5521 tgctcaatag cttgattgat aagtgttggt aatgaatcaa catttttctt tttcattacg
     5581 tagcgcatca tcatatttcc taaaccaaac atattcattt tggaaagagg cattcgtata
     5641 ttctttttgg gcaacattaa atcaaacatt tttgcaatac cttgcttttt aacattaact
     5701 gtttgcactt tttttaatgc attaagcccc caaaaagtaa agaagatagt tacatctttt
     5761 ccagcagctc tagcaccatt tgcaataatc aacgctgcta cagctttgtc taattctcca
     5821 ctaaataata caattgtagt acctttagca gaataattta tatctaaatc ttttgctttt
     5881 tctttttgaa taatcgcatt aattccattg ttattttcat caagcttaac taaagtatgc
     5941 cttgtttgtt tcacccaact tttaatgtca ctagggaatc cagggtctgt gactttgact
     6001 tcaatttggt cacctacttc aatattcttc atttcttgac taatttttac aattggccct
     6061 ggacattgaa gaccacgata gttaaattgt ttacgatctg ctttaatttc tatactttta
     6121 tattcttctt gtgtatcata actatcgttt tcttcttcat aagctttata accaccatca
     6181 agatttataa cgttataacc ttgtttagct aaatatttac tagcattagc acttctgtta
     6241 ccacttctac agattacata ataggtttta tttttttctt tattgaatga ctcaatctca
     6301 tcaacaggat gaagtatgga accattgata tgtcctaatg catactcctc ttctgttcta
     6361 acgtcaatta attgcccttg cttggctagt ttttccaatt ctgctttact aaattcatcg
     6421 ataaactttt ctccgtattg tttcataata acctccctaa aaatacctgt aggggtattt
     6481 taaacagttg gatggaactt gtcaaatacc tctaggggta tttgacacat ttgtgtttat
     6541 ctattaatat atttttattc aattaaaagt ggaggtatga ctatgaatta tgataaaaaa
     6601 atgattaatc gtattaatag aatacaaggg caactaaatg gaattattaa aatgatggag
     6661 gaaggaaaag actgtaaaga tgtcattaca caaataagtg catcaaagag ttcactccaa
     6721 cgcttgatgg gtatcattat tagtgagaat ttaatagaat gtgtaaaagc agctgcggat
     6781 gatgaagaaa gctcccaaga gttaattaat gaagctgtaa acttattggt gaaaagtaag
     6841 taatggatat tgttaatata gttatcatgt tactcattgg ggtattcggt gggttcatat
     6901 ctggtctagt aggcgtaggt ggtgctatca ttatttatcc agctatttta ttattaccac
     6961 cattattcgg agcccctgca tatagcgcat atatcgcatc aggtcttact tctagccaag
     7021 tctttttcag tacattaagt ggctcattaa aagctcgaaa aaaaacagag ttctcgccac
     7081 aattagttct ttatatgggt ggcggcatga ttatagggag tatgttaggt gcatttctag
     7141 caaatttatt tgatgctaca tttgtaaata cggtatatat cattattgct ttacttgcat
     7201 taacattaat gtttattaaa gtgaaaccta gttccgaaaa gtcttcattt aataaatatt
     7261 tattagttat cattggcatt ttcattggta ttatttcagg tatagtaggt gcaggtggtg
     7321 ccttcattat tattcctata ttattggttt tattcaaatt accaatgaat actgtagtcg
     7381 ctaatagtat agtaattgct tttatatctt caattggtgc ttttgtcatc aaacttattc
     7441 aaggttatat ccctttatat gacgccttat tccttataat agggagtatt atatttgctc
     7501 caatagggtt aaaattaagt aaaaaagtcc ctaacataat acaaaaatgg attatcagta
     7561 ttttaatcgt ttttgctata gttcaactca ttttataaaa ctaatccttt taactcagac
     7621 agcaatttcc cgaaaaataa tacctcttat ttaaacaaat atgtatgcag ttcggattta
     7681 tataaataaa aatttttgat gaaatatcca tatatgctta taaatataaa tttatcaact
     7741 ataactaaaa atgataaaag atttaacgcc actgatgacc gaattaatat tatttaagtt
     7801 tttatcaaac gcatgagtaa agtagatgca cattgggaat taatcgaagc tattaaaaat
     7861 ttacgtgacg aaattgcacc aaacacattg ttaacaatta acgatgatat tcccgataga
     7921 aaaacaggac ttgaacttgc agaaaaatat ggtattgatg gcatcatgat tggtagaggc
     7981 attttccaca atccattcac ttttgaaaaa gaaccacgcg aacacacaag caaagaacta
     8041 ttagatttgt tgagattgca tctatcattg tttaacaaat atgagaaaga tgaaatacga
     8101 caattcaaga gtttgcgtag attctttaaa atctatgtgc gtggcataag aggcgctagc
     8161 gaacttcgcc atcaattgat gaacacacaa tcaattgcag aagtacgagc actactagat
     8221 gaatttgaag cccaaatgga cgaagacgtt aaaattgaat tatagtatga gtaataaggg
     8281 aaaggaggaa tttaagtttc ctcctttgtt tgtgtgaaag tcttttaggt atgaactagg
     8341 gtgatttaaa ataaaagttt aatttctatc ttcagttggc gtttccatat aaataattgg
     8401 attaattctt tgatctttta aatagagaga ccactctact aatttatttt taagtaatac
     8461 tgaacctaaa ttagttaatt gaaagttttt atttattacg ttactagtat gaaccatatc
     8521 ttttatgggc atattatcaa tagatgatat cggtaaaatc catactatac taaaatttgt
     8581 actccgttgt atatcattaa atatattttc cgagttatca ggaatagctt ctattaattc
     8641 ttcaaaatta tatgattctg gatattttaa ggcgttaatt gctattatat tctgttctag
     8701 actataaaga gataaagatg tattaattcc tcttttaatc gtgaaaagct ctttgttttt
     8761 aatattgtat aagtctgcca actctattcc atttcctttt actataaaat tcttatttat
     8821 tggttttatg aattttttat gtaacattaa atattcttct ggcaatcttc ctactatttc
     8881 ttttatatat ccttcttctc cttctttaaa tactaaacct gtgggagtta gtgtgttata
     8941 cctaatttct atctcattca aatgatcatt caaaaggtca aaaaattctc tattaaaata
     9001 accccatttc ccattttgta ataaatattt ctttccatct tccatttcaa cttctgcatg
     9061 tagtatcgtt tttagtagca tattatttga atgccccaaa ttatctatca cttcaattct
     9121 gacatcattt atagaattaa cgtcatattt taataagtaa tctcctattt cagtgatgta
     9181 atcaacttca ggatc
//
LOCUS       DAKTGS010000116         9009 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.116, whole genome shotgun sequence.
ACCESSION   DAKTGS010000116 DAKTGS010000000
VERSION     DAKTGS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.116"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..440
                     /locus_tag="O0286_002203"
     CDS             <1..440
                     /locus_tag="O0286_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170562.1"
                     /translation="TLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMICS
                     RLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIALE
                     KWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            507..902
                     /locus_tag="O0286_002204"
     CDS             507..902
                     /locus_tag="O0286_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170563.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            1040..1339
                     /locus_tag="O0286_002205"
     CDS             1040..1339
                     /locus_tag="O0286_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170564.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            1419..1961
                     /locus_tag="O0286_002206"
     CDS             1419..1961
                     /locus_tag="O0286_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170565.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(2057..2623)
                     /locus_tag="O0286_002207"
     CDS             complement(2057..2623)
                     /locus_tag="O0286_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCY0170566.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(2625..3083)
                     /locus_tag="O0286_002208"
     CDS             complement(2625..3083)
                     /locus_tag="O0286_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCY0170567.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(3086..3769)
                     /locus_tag="O0286_002209"
     CDS             complement(3086..3769)
                     /locus_tag="O0286_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170568.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(3941..4816)
                     /locus_tag="O0286_002210"
     CDS             complement(3941..4816)
                     /locus_tag="O0286_002210"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCY0170569.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            5029..6660
                     /locus_tag="O0286_002211"
     CDS             5029..6660
                     /locus_tag="O0286_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCY0170570.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            6657..8330
                     /locus_tag="O0286_002212"
     CDS             6657..8330
                     /locus_tag="O0286_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCY0170571.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(8458..8901)
                     /gene="mgrA"
                     /locus_tag="O0286_002213"
     CDS             complement(8458..8901)
                     /gene="mgrA"
                     /locus_tag="O0286_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCY0170572.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         3069 a         1337 c         1532 g         3071 t
ORIGIN      
        1 ggacacttaa taaagatgaa cataattata tcaagcaaat agctaatata catgagacat
       61 tattgtcgca agtagaatcc aactataaat gtactaaact gagtattgct cttaggtacg
      121 agatgatatg ttcaagatta gaacatacaa atgataaaat ttatatatat gaaaatgaag
      181 gtcaattaat agcgtttatt tggggacatt ttagtaatga aaaaagtatg gttaacattg
      241 aactgctata tgttgaacca caatttcgca aactgggaat agctacgcaa ctgaagattg
      301 cgcttgaaaa atgggcaaaa actatgaatg caaagcgaat aagcaataca attcataaaa
      361 ataatttgcc aatgatatct ttgaataaag atttaggtta tcaagtgagt catgtgaaaa
      421 tgtataaaga tattgattag aattaggatt atgttgctaa ttcatgttaa aattaaaaaa
      481 gatttaatga cgttaaggag ttttatatga agaaattaat catcagtatt atggcggtca
      541 tgctattttt aacaggttgt ggtaaaagtc aagagaaagc cactctggaa aaggatatcg
      601 ataatttaca aaaagaaaat aaagaattaa aagacaaaaa agaaaagctt caacaagaaa
      661 aagaaaaatt agcagataag caaaaagacc ttgaaaaaga agtgaaagat ttaaaacctt
      721 caaaagaaga taacaaggat gataaaaaag acgaagacaa aaataaagac aaagataaag
      781 ataaagaggc atcacaagat aagcaatcaa aagatcaaac taagtcatcg gataaagata
      841 atcacaaaaa gcctacatca gcagataaag atcaaaaagc taatgacaaa caccaatcat
      901 aatcgaattg cttacttgtt atagatgaaa ggtacagcgt tttaaacctt attttaaggg
      961 tatgtattaa ttaaaatgtg gtcatgattg aaacagaacg taaaaataga caacgtaatt
     1021 aataaaggag agaaacggca tgcatgaaca agattttaga attttagagg gtcaagatat
     1081 tactttgcca gaattaggta gagaattaga aaatattaca ggacatacga ttgctgattc
     1141 tactggcgaa attaagcgtg taattgcaca tttaccaaac tttgagtccg atacagatac
     1201 ttttgttgct acatatcgtt taaaccatca acaagatttt atagatgcaa cttttactgc
     1261 gctgaaatca gatagagcac gtttaaaaga agtgccagtt catgttgaac ttataagtta
     1321 tatttctaaa tcaaaataaa ctgctatcta aaacgcaaag ttgatcaaaa tatcgatttt
     1381 gtgtttttta ttgagaaatt atataggagt gtcaatcgat gatttattgt gaaacagagc
     1441 gtttaatatt aagagactgg catgaagatg atctgttacc ttttcaaaaa atgaatgcga
     1501 attatgacgt acgtaaatat tttccaagtt tattgagtta tcgtcgttca gaattagata
     1561 tgagaactat ggatgcggtt attaaagatt atggcattgg attatttgct gtagaagata
     1621 aagagtccca tcaatggata ggctttatag gtttgaatta tattccagaa acaagcgatt
     1681 atccatttaa agaattaccg ctttatgaaa taggttggcg cttgttgcca gaattttggg
     1741 gaaaaggatt agcaactgaa ggcgcaaagg caacattgaa gttagcagaa gaacatcaaa
     1801 tatacgatgt ctatagtttt acagcagaag caaataaagc ttcacaacgt gtaatggaaa
     1861 aaattggcat gacagtgtat gatcatttcg aattacccaa tctaagtaag tatcatttat
     1921 taaaaaggca agtgcgctat tacattaatc ttcgaaagtg aaaaatttat acataagcgt
     1981 aacaaacacc cctaacattg tttagctgat gataacaata ttaggggtgt ttgtttattt
     2041 tttaacctta gaatgattaa tcgtatgaac gagtacccag aggtttgaaa tttaatattg
     2101 attcaattaa tgattcctta gtgtcgcata acggtgcaag agcacgatac ttaggatcaa
     2161 taaaaccttc ctcaatcata tggtcaatca ttgtttgtag tggattgaaa aagccattaa
     2221 tattataaat ggcaataggc ttttcatgga tacctatttg agcccaacta tacatttcga
     2281 aaaattcttc tagtgaacct gcgccaccag gagccatgac aaatgcatct gcaagttctg
     2341 ccattttatt tttacgttca tgcatagaat caactaaaat taattcagtt aaacgttggc
     2401 ttgtgatttc atgttcatct aacattttag gcatgacgcc aatagctttg ccgccatgat
     2461 ctaatacacc atcttgaatg gcacccataa tgccaattga ccctgcacca aatactaatt
     2521 cataaccttg ttcagcaaaa tatttaccta aatcgtatgc tttttgtaca tatgaagggt
     2581 catgaccttt gcttgcacca caataaactg cgattcgttt catgttaatc cagctcctta
     2641 attcgatgaa tgacttttaa tagtgattgt tcaaacactt tttgatcttg ctttgtaaaa
     2701 ggtgggggac ctttgtggcg accaccttgt tttctaattt gtgcattcat atatcgttta
     2761 tctaatagtt gttgaatatt tttggaattg tatatcttcc cattatgatg catgacaatt
     2821 aagactttgt cgactaataa acttgcgagt ccataatctt gagtgactac gatatcatcc
     2881 ttcgttgata attgaacaat tttgtaatca actgcatctg gtccatcatc aacatataat
     2941 gttgatacat gtggaggata taattggttc gaaaaatggc tgaagctccg aataattgtc
     3001 acaaaaatgc ctgtctcagt tgttaaatct ataatagaat caacaacagg acaagcatct
     3061 ccatcaataa taatatgtgt cacaattatg cctctgtatt gttttcttta ttttgttgag
     3121 aggcgctttt ggcaacataa tctttatatt ttttaaatga cttgatgcgt gctttatcag
     3181 cttcttgttg gcgtttttgt tcttctttgt gtcgtttttc aatatttttt tgtaactttt
     3241 tattcatttt agcgatttct ttgcgatttt tttcagctag tttatcgcct tttttctcag
     3301 ttttctcatc taatttatta ggtgttaagc ctgctttttc ttcgtatttt tgtgattttt
     3361 tcatatcttt aatacgttgt atttcattct tttcgcgggc tttttgctct tctttatgac
     3421 gcttttcgat atttttttga agtattttat tcattttatc agcgtcttta cgattttgtt
     3481 tagctaattt ttcgcctttt ttctcaatat aggcaggatc atgttctcta gcaaactttt
     3541 taagttcacg tttattttca aaatcttgtt ttttatcgcc gacatattct ttaacatcac
     3601 tcgctgtgtt actgattgct gcagatgttt ttgaagcaac tttacttgta gcatctgtaa
     3661 ctttttgtac gtccggatgt tgtttgatac gtttacgttc aacaattaac ggtaccaata
     3721 caattggtaa tacattaatc ataaatttga tgactttttt cttatccata gatcttgcct
     3781 ccataattac tttattaatt ttacataccc tatgatacat caatataaac gatgatagta
     3841 gtgaatcact attaagtatt tcagatgttt tttaaaagaa gacaataaaa actgccaatc
     3901 aagtgattcc ttaattgaca gtctatattt taaaaggaaa ttaaatacct ttaccaatgc
     3961 caaatccgaa gtaaagtata gcaataaaga ttactaatac aattctgtaa atggcaaatg
     4021 gaattagttt gattttgtta attagatgca agaatgtttt gattgcaatt agtccaacag
     4081 taaatgcagc taaaaagcct aaaatataaa aaggtatatc agcaatctga atatcttgat
     4141 aatgttttaa taaagataaa ccactagctg ctaacataat tggaacagcc ataataaatg
     4201 taaagtccga tgctgcttta tgatttaatt tcattaatac cccagttgaa attgttgagc
     4261 ctgaacggct gaaaccaggc cacatagcta ctgcttgaga aataccaatt acaaatgctt
     4321 ggaaataact gatttgatct actgtttgtg ggtttttaac tttagctgag tatttatcag
     4381 caataatcat atagatagca cctacgaata agccaatcat aacagttggc acactaaata
     4441 aatgttcttc gatgaaatca tcaaatagta agcctaaaat acctgctggc accataccca
     4501 ctaatacatg taataaattt aaacgtcttg gctttgaacg tctttgttga tcgttatctc
     4561 cttcaacatg tttgtgttta ccaatatgta aaatctctaa gaagcgttcg cggaacaccc
     4621 atgctgctgc aaagacggat cctaattgga tgacgatttt aaatgtaaat gctgactgag
     4681 aacctaaaaa ttcagatgat tttaaccaca tatcatcaac taggatcata tgtccagtag
     4741 aggaaacagg tgcaaattct gttaatcctt cgacgacccc taagataata ccttttatta
     4801 attcaatgat aaacataatg tacccacttt cattactcaa tttaatttat ttaaatatca
     4861 aaattaccat atcatgatag catattcatt taaagacatg ctagttataa tactagacta
     4921 aagatgtata tattcatttt cttttacatg taaaactaca atattttatt gagctattta
     4981 atttgatttt aaggaaaacc ttttataata ggtttaggtg atataattgt gaaaaaatta
     5041 acaacaatac tgtttcaata taaaattttt ccggtactca tgttcttggt cagtactggt
     5101 ctcggcataa tcgttataac gcaaaatatt ttaatagcag attttttagc taaaattata
     5161 agacatcaat ttcaaggttt atggattgta ttatttattt tattaggtgt tttactttta
     5221 agagcaactg tgcaatttct aaatcaatgg ttaggtgata cattagcatt taaagttaag
     5281 catatgctta gacagcgggt tatttataaa aataatggtc atccaatcgg tgaacaaatg
     5341 actatactca cagaaaacat tgatggtcta gcaccttttt ataagagtta tttgcctcaa
     5401 gtgttcaaat caatgatggt tccgctcatc ataatcattg caatgttttt catccatttc
     5461 aataccgcat taattatgtt aataactgca ccatttattc ctttgtttta tattattttc
     5521 ggtttgaaaa cgcgagatga gtcaaaagat caaatgactt atttgaatca atttagtcaa
     5581 cggtttttaa atattgctaa aggtttagtg acgttaaagc tatttaatcg tacagagcaa
     5641 acagagaagc atatttacga cgatagtact cagtttagaa ctttaacaat gcgcatttta
     5701 cgcagtgctt ttttatcggg attaatgctc gaatttataa gtatgttagg tattggattg
     5761 gttgcattgg aagcaacgct aagcttagta gtatttcata atattgattt taaaactgcg
     5821 gcaattgcga ttattttagc gcctgaattt tataatgcaa ttaaggactt agggcaagcg
     5881 ttccatactg gaaaacaaag tgaaggtgcc agtgacgttg tgtttgagtt tttagaacaa
     5941 ccgaactata ataatgaatt tctattaaag tatgaggaaa accaaaagcc atttattcag
     6001 ttaacagaca tatcatttcg atatgatgat tctgatagat tggtattaaa tgatttaaat
     6061 ttggaaatat ttaaaggtga tcaaattgca cttgtaggtc caagcggggc aggtaaatcc
     6121 actttgacac atcttattgc aggtgtttat cagccaacaa taggtactat aagtacaaac
     6181 cagcgtgatt taaatatagg aatacttagt caacagccat atattttcag tgcttctata
     6241 aaagagaata ttacgatgtt taaagatata gaaaataata ctattgaaga agtgctagac
     6301 gaagtaggtt tattagacaa agtgcaatct ttcacaaaag gcattaacac aataataggt
     6361 gaaggaggcg aaatgttatc tggtggacag atgagacgca tagaactttg ccgtctttta
     6421 gttatgaagc cagatctcgt tatatttgat gagcctgcaa ctggtttaga tattcaaaca
     6481 gaacacatga ttcagaacgt tctgtttcaa cattttaaag atacaacgat gattgtcatt
     6541 gcacatagag ataatacaat tcgccattta caacgacgct tgtatataga aaatggaaga
     6601 ctgattgctg atgatcgcaa tatttcagta aatataacag aaaatggtga tgacttatga
     6661 aaacacgact aaaatttcaa gtagataagg atttattgtt agctatagtt gttggtgttt
     6721 gtggaagttt agttgcgctc gccatgtttt tcttaagtgg ttatatggtg acacaaagtg
     6781 cacttggtgc gccactatac gctctgatga ttttagtcgt tacagtaaaa ttgtttgggt
     6841 ttttaagagc tattactcga tacgtagagc gccttatttc tcataaagct acatttacaa
     6901 tgctacgtga tattcgggta cagtttttcg gtaaattagt aaatgtcatt cctaatgttt
     6961 accgtaaact gagttctagt gatttaattt cacgtatgat tagtcgtgtt gaggcattac
     7021 aaaatatata tttacgtgtt tattatccac cagtcgtcat cggtttgaca gcgctagtta
     7081 cagtcatagt tttggcgttc atttcaatcg gccatgcgct attgattatg gttagcatgt
     7141 tgttcacttt actcattgtt ccttggttaa gctcaaaaaa agcacgtact ttaaagaaac
     7201 atgcagctaa tgaacaggcc cgatttttaa atcattttta tgattataaa gctggtatgg
     7261 atgaactacg tcgatttaat caaattaatc attatcgaga taatttgatg gctaaattaa
     7321 atcattttga taaattacaa cttaaagagc aacgcttttt aacgatttat gattttatat
     7381 taaatattat tgctatgctt tcgatttttg gtagtttagt tctaggatta attcaaatta
     7441 atgcaggcca actaaatatt atttatatga cgagtatagt tttaatggtc ttaactttat
     7501 ttgaacaagc tgtaccaatg acaaatgtcg cgtattataa agcggatact gaccaagcat
     7561 tgcacgatat taatgaagtg atatctgtac cttctactaa tggaaaaaaa cgtcttaatg
     7621 ataagtatga tgcaacgaac atttatgaag ttaaggatgc tagttttaag tattggaatc
     7681 agcaaacgta tgtgttgtcg gatattaatt ttaatgttaa tagaggcgaa aagattgcga
     7741 ttgtgggtcc ttctggttca ggaaaaagta cattactaca aattatggca gggttatatc
     7801 aattagatag tggctctgtt cgtttcgaaa atatggatat gtttgaaata gatgacaaag
     7861 ataagtttga atcgttaaat gtcttgctac aatctcaaca attatttgat ggtacaatac
     7921 gtcaaaattt atttaccgat gaaaaagatg aagcggtgca agcaatattt aagcaattag
     7981 atttagaaca tttggcacta gaacgtcaaa ttgacttaga tggtcataca ttatctggcg
     8041 gagaaattca gcgtttagcg attacgagga tgttattaaa agatactgca tcaacatgga
     8101 ttttagatga accaacaact gcattagata aacaaaatag tttaaaagtt atggatttaa
     8161 ttgaagcaca tgcagaaaca ttaattgttg ctacacacga tttaacttta ttgtcacgtt
     8221 ttgagaccat cattgtgatg ataaatggta aaatagttga aaagggaaac tatcaacaat
     8281 tactagctaa tcaaggtgct ttatggaata tgattcaata taatgcataa aaaaaactgc
     8341 ttgataggct aagaaacctt tcaagcagtt ttttaactaa caagcttacg cgtgctaatt
     8401 gtttttctat tcttaataaa ttctaaacat tactttaatt gtcatgacaa aagttaatta
     8461 tttttccttt gtttcatcaa atgcatgaat gactttacct aataagcgat taagttcttt
     8521 aacttcatct tgcgataaag aagaagctga agcgactttg tcagatgcat tacttaattc
     8581 tggtctaata gtttcacttt tgtcagtcaa gtgaataaat acttcacgtt gatcgacttc
     8641 ggaacgttca cgcttaatta agtctacttg ttccattcgt tttaataatg gtgatactgt
     8701 accagtatcg agtgctaatt cagttacgac tttcttgacg tttacaggag attcatccca
     8761 taaaattgtt aagacaagaa attgtgggta tgttagattg tacttcttaa aaactttgtt
     8821 agagtagtag cgattaactt gtctttgagc attgtacaaa ctaaagcata gctgttcttt
     8881 taaattatgt tgatcagaca ttaaagttct cctccagaca tactatccgt ttttttctct
     8941 tttcggattg gtaatcatta aaaagttgat tgtttattaa ttcacaactt tctttgattc
     9001 aatgccatg
//
LOCUS       DAKTGS010000117         9005 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.117, whole genome shotgun sequence.
ACCESSION   DAKTGS010000117 DAKTGS010000000
VERSION     DAKTGS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.117"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..69)
                     /locus_tag="O0286_002214"
     CDS             complement(<1..69)
                     /locus_tag="O0286_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0170573.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELR"
     gene            396..1139
                     /gene="sarU"
                     /locus_tag="O0286_002215"
     CDS             396..1139
                     /gene="sarU"
                     /locus_tag="O0286_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCY0170574.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSHADERIVL
                     IKINKIQYNTIKSIFTDTSKILKPRKFFF"
     gene            complement(1350..2216)
                     /gene="galU"
                     /locus_tag="O0286_002216"
     CDS             complement(1350..2216)
                     /gene="galU"
                     /locus_tag="O0286_002216"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCY0170575.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(2396..5218)
                     /gene="fnbB"
                     /locus_tag="O0286_002217"
     CDS             complement(2396..5218)
                     /gene="fnbB"
                     /locus_tag="O0286_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCY0170576.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(5900..8914)
                     /gene="fnbA"
                     /locus_tag="O0286_002218"
     CDS             complement(5900..8914)
                     /gene="fnbA"
                     /locus_tag="O0286_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCY0170577.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKPQSKKSELPETG
                     GEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(8898..8999)
                     /locus_tag="O0286_002219"
     CDS             complement(8898..8999)
                     /locus_tag="O0286_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170578.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
BASE COUNT         2463 a         1682 c         1304 g         3556 t
ORIGIN      
        1 acgcagctca tatgtagtta atactctttt cattaattta atattgctct tgcttttcaa
       61 atcattcatc aagtcttcat cccttacttt taattgtcac gtcaaataat atttgtcttt
      121 aactagtaat aactccaaat atatttagtt tgtggtgcta gctataaaca caaatgaatc
      181 ataacactat attttgattt tgttcaattg tttttatttt tggataataa atgaatttgt
      241 catttgttat tctgtgattt ttagttattt atattttata tatatatatt atatataaaa
      301 tatttaggtt aacatgtctt taaaagagat tgcttttata ttactttaaa taatataaag
      361 tattgggaca tcagaaaata gtaggagaat gtaaggtgga ctatcaaact ttcgaaaagg
      421 tcaataaatt cataaatgta gaagcgtaca tattttttct cactcaagag ttaaagcaac
      481 aatataaatt atcattaaaa gaattgttga tattagcata tttttattac aaaaatgaac
      541 acagtatttc actaaaagaa atcattggtg acatacttta caaacaatct gatgttgtaa
      601 agaacattaa gtcactatct aaaaaaggat ttataaataa gtctagaaac gaagcagatg
      661 aacgccgtat ttttgtttca gttactccaa tacaacgtaa aaagattgct tgtgttatta
      721 atgagttaga taaaataatt aaaggattta ataaggaaag agactacata aaatatcaat
      781 gggctccaaa atatagcaaa gaatttttta tactttttat gaacattatg tactcaaaag
      841 attttttaaa atatcgattt aatttaacat ttcttgattt atctatctta tatgtaatat
      901 catctcgaaa aaatgagata ctaaatttaa aagatttgtt tgaaagtatt agatttatgt
      961 atcctcaaat tgttaggtca gttaatagat taaataataa aggtatgcta atcaaagaac
     1021 gatcccatgc agatgaaagg attgtgttaa tcaaaataaa taaaatacaa tataacacta
     1081 ttaaaagcat attcacagat acttccaaga ttctcaaacc aagaaaattt ttcttttaaa
     1141 tttaaacaga tttacctctt gataaaataa ataagcaatc atactacttc tcaatttagt
     1201 ataaataaaa atacataatt aactttcttt tgtttttata ttatttcaat accctactat
     1261 atatcacaac acataaatta agcatgacac tcattcaatt tagttcacca tttcgtgttc
     1321 caattttact gagtatcatg cttttaatgt tataaaccta atgctttaat aaatcgtgtt
     1381 aattcttctc gcatactgtc atctttcaat gcatattcta tggtagtttt aacgaagcct
     1441 aatttttctc caacgtcata acgttcgcct tcgaagtcat atgcatacac ttggttatca
     1501 ttattcatac gttcaatcgc atctgttaac tgaatttcgt tacctgcgcc ttctttttgc
     1561 gtttttaaat aatcgaaaat ttcaggcgtt aatacataac gtcccataat agctaggttt
     1621 gatggtgccg taccttgtgc tggcttttca acaaactttt tcacttcata ctgacgtccg
     1681 tttttagtta atgggtcaat aattccataa cgatgagtat ctgcttccgg aacttcttgg
     1741 acacctataa ctgagtgccc tgtttcttca taaacgtcaa tcaactgttt cactgctggc
     1801 acttcagatt caacaatatc gtcacctaat aataccgcaa atggttcatt gccgataaat
     1861 tgacgcgcag aactaatcgc atgccctaaa cctttttgtt ctttctgcct tacataaaaa
     1921 atattcgcaa gttccgttga atactgaact ttctctagta attcagattt acctttttct
     1981 tttaacacca tttctaattc tttttgacta tcaaaatgat cttcaatcgc gcgtttgtgg
     2041 cgacctgtca ctataataat atcttcaatt ccagctcttg cagcttcttc aacgatatat
     2101 tgtattgtgg gtttatctaa gataggaagc atttcctttg gcatcgcttt agttgctggt
     2161 aaaaatctag tccctaaacc agcagcggga atgattgcct tttttatttt tttcaaagtt
     2221 aatgtgctcc ttttcctaag tattaaatct atgtatcaac gtcattttaa cactaattag
     2281 aacgccttca tagtgtcatt gagtatgtaa ttatttcttg ggaaatttgt tttaatttta
     2341 aaaaacaggc ttacttcata taatttatga aataaacctg tcaattttgg attgattatg
     2401 ctttgtgatt ctttttattt ctgcgtaata acgctaaacc taaaatgcta aataatccgc
     2461 cgaacaacat gccgttgttt gttgattctt ctccacctgt ttcaggtagt tcagatttct
     2521 tagattgtgc ttttttagtt ggtaccactg ctttaacctt ttcattgatt tcaataacag
     2581 gtgttactac tttaccttgt tccactggtt tagaaggttt tttaggttct tctttagcag
     2641 gtggtattgg tttaccaggt tcagttggta cctctggcgt tggcggtgtt ggtgtttccg
     2701 gctcgcttgg tacttctggt gtcggtggtg ttggtgtttc cggctcgctt ggtacttctg
     2761 gtgtcggtgg cgttggtggc acgattggag gtgttgtatc ttcttcaatc gtttgttgac
     2821 cttcattatg accacttact tgtggaagtg tatcttcttc aaagtcaaca ctattgtgtc
     2881 caccgaattg ataatttggt ttatctttat ttgtatcttc ttcaataatt tcagtgtgct
     2941 tattgaatcc gtgaatatgt ggcacactgt cgaagtcgat atcaatgata ttaccacctt
     3001 gttcatactt aggtttgtct ttctctgtat cttcttcgaa tgattggtta ccattatttt
     3061 gaccatgaat ttgaggtaca ctatcgaaat cgatatctac gatattgcca ccttgttcat
     3121 atttcggttt atcttcttct gtgtcttcct caaatgactg attaccgcta ttttggccac
     3181 cttcgtaacc taattcactc ttaatatcca cgtggctatt ttcttcgatt tcttcaatca
     3241 cgccataatt accgtgacca ttttcagttc ctaaaccaga atgagaaata tgatgattgt
     3301 tttcagtaat ttcctcgatt ggtccttgcg cttgaccatg ttcttcaggt agttcatcta
     3361 ctagttcaat cagattactt tcagtcgtat attctttcgt atcttcaatt gttgtatgat
     3421 cgctaacagc accagttaca ataccttttg tagaatcttc gtcaaattca actaggttag
     3481 actcagtagt aacctgacca ccacctgggt ttgtatcttc ttcatattca acaacatcag
     3541 catgatgttt tgaattttca tgtgtcgatt cttcaaagtc tacatgaata gaatcttctt
     3601 cagtttcaat ggtaccttct gcatgacctt ctgcaccttc aacagctgta tgatattcaa
     3661 aatcaattgg cttagaatca ttactttctt cgattgtacc agtcaattca tgcttctcca
     3721 ctggcggctc tgatttaaat tcaagttcga taggagtact atgttctata ataggttcct
     3781 ttagtttatc tttgccgtcg ccttgagcgt tattagagta aaatgcaacg ccatttttcc
     3841 aagttaaatt acttgtataa taatagttat aatatccaaa aaggtgtgtt tgaaattcta
     3901 agttgctagc atttgaatca taataccctt catattttat tacataattt ttactttggt
     3961 ctaaattatt aaagtttaaa gaataaccac cattagtatc aaaatctaaa ctcatattat
     4021 cagtcacatc ttcaaatttg ctgacatcat caagctttgc atatacgctt tcagctaaat
     4081 cgtctgaacc aatgtgttta tataccttaa ctgttggatt attaacccct ggtttatttc
     4141 ctttagttac ttgaccagtt actgtcacag agcttaacga ctggttgtta ggtttcatgt
     4201 acgcaaaatg actaaatttc ccatctactt tatttaaagt atcaattcga ccattagctg
     4261 ttactcccca attatctcta actccaccta aatattgaat attaaatatt ttgctaaccg
     4321 tagtctcacc caatttaact tcaacatttt ggttaccttt ttgcgtcact gttgtaggat
     4381 caataaatag atttaaagat aattcagcag ttaaatcttt cttttcttgt acatattctt
     4441 taaacgtata tctaactttt ctttctccaa ttatttctcc tgtcgccata acttgaccat
     4501 ctgtactttt tatctccgga actttacgca gtgttgagat accatgagtt tcaacattat
     4561 cgcttaatgt gaaatcaaaa taatctcccg ccttaattcc ttctccaaat ttccatttat
     4621 atttcaaggt tactctttct gcgttatgag gatttacaac attcgtatct tgtttatgtc
     4681 ctacaatttc actaccttct tctacttcca ctttatttgt tacatctgta cctgtcgctt
     4741 tagtttcttc cactacttct ttctctgcaa ctgctgtaac gtcagttgat cttttcattc
     4801 ttggtttaat ttctgagacg ttacttggtt gagctatgtc aacttgagtt cctgtagttt
     4861 ccttatcagc aactttttcc gatggcaaat caactcgcga agtttctact tttggtgctt
     4921 gcacagtttt cggtgcttct tctgttgtta cttgtgttga ttgtgatggt tgctcagttg
     4981 atgtcgcgct gtatgattgt gtttcatcta ttgtattaac gttatttgta gttgtttgtg
     5041 tttcgcttgc tttactttca gtagctgaac tcccactttc ctctactgta gtattgtttt
     5101 gttccgatgc tgcagcttct ttttcttgtc ccattccaac aacgatcatt gttcctaaga
     5161 atactgaggc cgctcccaat ttgtgttttc ttatgccgta tctaagattg cttttcacta
     5221 taatattctc ccttaaatgc aaaattcatt tatttttaaa actcaataaa tgcaattcta
     5281 tattgttcgg tttttaaaag caatgaaaaa aagcgagtta ataaaaagtt aagattgttg
     5341 ttaactttat gtataatgag ttttttatta tttgaaactc acatatatat tgcatacaaa
     5401 gctcttgaac accttgatat aacaggcttg tattttttat acttactttt taaattaaat
     5461 tcaaattcat ctaatttaaa acaatatact aaaccataca taataatcgc ctgtacaatg
     5521 catcattaac aagtcactga aacgcctttc attgtattaa taacgtcact ataattttta
     5581 tatcgttcgg tttttgtttg attttaatga ttatttatac aaaaacagcc gtatttcaag
     5641 ccgacatttt aaatttaact aaatttgcat ctagttaata attgcattta tcaaatttgt
     5701 cttattgatc caatctaatt tgtactcaca aactagttta aaattctaac tttatctctc
     5761 agttcgttat caatcatcag acataaacca atgaagcaat cagaaaacac tctaattttc
     5821 tattagaaat ttgatttaat ataaaaaaac aggcttactt catataattt atgaaataaa
     5881 cccgtcaatt tttgtttaat tatgctttgt gattcttttt atttctgcgt aataatgcta
     5941 aacctagaat gctgaataat ccgccgaaca acataccttt gtttgttgat tcttctccac
     6001 ctgtttcagg tagttcagat ttcttagatt gtggtttttt agttggtgcc actgctttaa
     6061 ccttttcatt gatttcaata acaggtgtta ctactttacc ttgttccact ggtttagaag
     6121 gctttttagg ttcttctttg gcaggtggta ctggtttacc aggttcagct ggtacctctg
     6181 gtgttggcgg tgttggagtt tctggctcac tcggcacttc tggtgtcggt ggtgttggtg
     6241 tttccggctc acttggtact tctggtgtcg gtggcgttgg tggcacgatt ggaggtgttg
     6301 tatcttcttc aatcgtttgt tgaccttcat tttggccgct tacttttgga agtgtatctt
     6361 cttcaaagtc aacactattg tgtccaccga attgataact tggtttatct ttatttgtat
     6421 cttcttcaat aatttcagtg tgcttattga atccgtgaat atgtggcaca ctgtcgaagt
     6481 cgatatcaat gatgttaccg ccatgttcat acttaggttt gtctttttct gtatcttcct
     6541 cgaatgactg attaccttta ttttgaccat gaatttgagg tacactatca aaatcgatat
     6601 ctacgatatt gccaccttgt tcatatttag gtttgtcttc ttctgtgtct tcctcgaatg
     6661 actggttacc gctattttgg ccaccttcat aacctaattc actcttaata tcaacgtggc
     6721 tattttcttc gatttcttca atcacgtcat aattcccgtg accattttca gttcctaaac
     6781 cagaatgaga aatatgatga ttgttttcag taatttcctc gactggtcct tgtgcttgac
     6841 catgctcttc aggtaattca tccactaatt caatcagatt actttcagtt gtatattctt
     6901 tcgtatcttc aactgttgta tgatcgctca ctgcgccagt tacaatacct tttgtagact
     6961 cttcgtcaaa ttcaactaag ttagactcag tagtaacctg accaccacct gggtttgtat
     7021 cttcttcata ttcaacaaca tcagcgtgat gttttgaatt ttcatgtgta gattcttcaa
     7081 agtcaattgg atttgattcc tcagaggact cagtgtatcc tccaacgtga cctgcttcgc
     7141 tatccacagc agtatggtaa tcgatatcaa tagctgatga atccgtttct tctattgttt
     7201 caatgtatcc atcaacatat ccacctccac catctatagc tgtgtggtaa tcaatgtcaa
     7261 gagttgatga atcatattcc tcttcaacag tagttactaa attcttatca tattgacctg
     7321 taagagtttc tttaattgta tcttctttat attcaaattt attattttga ataatcggac
     7381 catttttccc atttccgttc gctttattac tgtataaaac taaaccatta tcccaagtta
     7441 aggtatatcc tctatcataa taatacttat aaagttgctc tggatgtcct accatttgtg
     7501 ttctaaaatc aacttcatca gtaccattta aatactctcc atcatagtga acaacataag
     7561 ttttatctag attttctata ttcaatgaat agcttccatt attttgtaaa ttcaaattcc
     7621 cactcatatt acttgtgact tctttaaatt tagaagtatc tgtcgtattt gcatatacac
     7681 tcttcgctat gtcttcatta ttacccaagt attcaaatat cctaactttt ggttgatttc
     7741 cattctgatt actacctttc attaaagttc cagtaacagt cacacttgtc gttttaccat
     7801 tattaggttt aataaatgca acatgcgaaa atctattatt cgctttatta aatgtctcaa
     7861 tcgatccatt taaattggca taataattcc caataccatc tttatattta acatctaatt
     7921 cctttgaagt ttgttcttca tttagtgttg aagttatagt ttgatttcca ttagtttgta
     7981 cagttttagg atcaataaat aaattaattt ctagttcagc cgttacatca accttatctt
     8041 caatatcatt tgtaaatgta tatctaatct ttccaccttc taaaacttca cctgtcgcca
     8101 ttacgactga accattttta atttctggta cttttctagc agttgatacg ccatgcgtat
     8161 ttacattatt tgataaagta aagtcaaagt agtcaccttg atgtaaacca ttctcaaatt
     8221 tcaacttata ttttagtacc gctcgttgtc ctgcatgagg ttctacttta tttgtattgt
     8281 tatgcccctc aatagaacca atttctactg taactttact tgttacatct gtacccgttt
     8341 ccactttcgc gttactagct tccttagctt ccgctacatc tgctgatctt gtcacacgtg
     8401 gcttactttc tgatgccgtt cttggctgtg ccacttcaac ttgtgtttct gcgacttgat
     8461 tttgtgtagc ctttttaggt gttaaatcta cttgtctttg atctccgcta ttgtcttgag
     8521 attgtgttgt ttccttaact tgaggtttcg cttcttcctt aactacctct tctttaactg
     8581 tttctatatt tgctggttgt gcagtttgtg gtgcttgtac tgcttttggt gcttcttcag
     8641 ttgttacttg tgttgcgttt gacggttgtt ctgttactgt tgcgttatat gattgagttt
     8701 cttctatatg attaacgtta gttgcagttg tttgtgtttc acttgtttta ttatcagtag
     8761 ctgaattccc attttcttct actgtagttg tcttttgttc tgatgctgca gcttctttgt
     8821 cttgtcccat cccaacaacg atcattgttc ctaagaatac tgatgctgct cccaatttat
     8881 gttttctaat gccgtaccta agattgtttt tcactataat atctcccttt aaatgcaaaa
     8941 ttcattaatt ttttaaactt aataaatgca agtctatatt gttcggtttt taaaagcaat
     9001 acaaa
//
LOCUS       DAKTGS010000118         8874 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.118, whole genome shotgun sequence.
ACCESSION   DAKTGS010000118 DAKTGS010000000
VERSION     DAKTGS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.118"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            27..2176
                     /locus_tag="O0286_002220"
                     /pseudo
     CDS             27..2176
                     /locus_tag="O0286_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2322..2681
                     /gene="sarX"
                     /locus_tag="O0286_002221"
     CDS             2322..2681
                     /gene="sarX"
                     /locus_tag="O0286_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCY0170579.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDD
                     ISKFNALFEDVEQFLNI"
     gene            2871..3587
                     /locus_tag="O0286_002222"
     CDS             2871..3587
                     /locus_tag="O0286_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0170580.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            3587..4060
                     /locus_tag="O0286_002223"
     CDS             3587..4060
                     /locus_tag="O0286_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCY0170581.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            4152..4268
                     /locus_tag="O0286_002224"
     CDS             4152..4268
                     /locus_tag="O0286_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170582.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            4413..5057
                     /locus_tag="O0286_002225"
     CDS             4413..5057
                     /locus_tag="O0286_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0170583.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            5173..6039
                     /locus_tag="O0286_002226"
     CDS             5173..6039
                     /locus_tag="O0286_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0170584.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            6309..7529
                     /locus_tag="O0286_002227"
     CDS             6309..7529
                     /locus_tag="O0286_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCY0170585.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            7526..8014
                     /locus_tag="O0286_002228"
     CDS             7526..8014
                     /locus_tag="O0286_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCY0170586.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(8113..8802)
                     /locus_tag="O0286_002229"
     CDS             complement(8113..8802)
                     /locus_tag="O0286_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCY0170587.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
BASE COUNT         3253 a         1119 c         1519 g         2983 t
ORIGIN      
        1 acttaaatat agaaagaggt aaagttatgg caaaatcatg cttgcatata cttactaata
       61 atgaatatgc gacaacgcgt tgccaagatg gcatagtctt attttggcca attgacgggg
      121 aaatcgaact acaaaaattt cgtaaaagta aaataattga agatgatata tatattatta
      181 atcatctgga tgtatttagt attaagaata ataaaaaaac gatcatgttg tatttgagta
      241 gcgattggtt tgcggaatta ggctttactt tctttaatta ccactataca gcaaagttga
      301 ttaaatcatc ctataatttg aaatgtctac tattaaaatt gacatatcga taccttgata
      361 atcagcctct taatgacgct gatattagaa aattacagga tattattaaa atcattgcaa
      421 aagaagcaag tatggataaa aagattgcac aaaatcaata tcgatatgcg tattatggtg
      481 atttgcgtga tgagctcgaa tatatttatc aaaatgtaaa tcaacgattg acattaaaaa
      541 gtgtcgctga taaattattt gtctcaaagt caaatttgtc atcacaattc cacttactta
      601 tgggcatggg ttttaaaaaa tatattgata ctttgaaaat tggtaaatcg attgaaattc
      661 tacttactac tgatagtact attagcaaca taagtgaaca tttaggtttt agtagtagct
      721 ccacttactc taaaatgttt aaaagttata tggatataac accgaatgaa tatcgtaatt
      781 tatcaaaata taataaatgt ttaatgctaa agccagaacc actagtaggc aaaatggtgc
      841 aagaagtaaa agaaatcata ttgaattata ttgaacatta taaaaaccac ctaactgatg
      901 ttatacatat tgatgaagac aaatttgaaa cacctaaatt gtttcaaacg gttattcaaa
      961 taaatactta tacagaaatg aaattagttt tcttagaagg aatctttaaa accttattga
     1021 ataagaacag tcaagttgtc tttttcatca tgccatcgat tctaaaaagt aaaaatacca
     1081 tgtccgaaga agaaaaattc acaatcatta aaacaataat tgaaagtgat ctaaagatag
     1141 catttaatat aaataatatt gaaacaactt attttgttga agaagctttt atgagtgttt
     1201 tcagacaaat atctccaaac gaattaagta atcataataa ttacgaagtg cattttgttt
     1261 ttgatttatc attgatggaa attagaacaa tttatcgaat gatattaaaa ttacataaca
     1321 tcatgttgaa tgtgaaatta ggattgaaca ttacctgttt atttgaaaaa ccttcagttt
     1381 ttaaatcact agtatcacaa ataaagcgac ttaaattcga ttcgttaata atagataatg
     1441 caaatttaag tagcccttat ttgatggggg aaagtgatga gttactattg aaaaatattt
     1501 tgcattttaa aaatttaaaa caagtaatta atgaattgga tattgaacaa gaaaagctga
     1561 tttttctaaa tgttgaaaat cataaactgc ttaataataa agaacgagat ctaagtaata
     1621 gtgctccatt aatttataag acattaagtg cgctgtatca caactttgat ggctttggat
     1681 taaacatttt tgacaatcat caagcattta atgcgatgca tctatatgac aaaaatggat
     1741 ttaaaacaac actaggtctt atattagaaa agtttatcga atatgtctcg aaaccaaaat
     1801 acgaaaacag ttattattct atttttgata tagagaatta ttattgtctt gttatttatg
     1861 attggcgagt gatagagagc gagacaatta tgagtaattt tgaggatagt caagtttata
     1921 taaattttaa aaacaatgtt ttaaacgata aatatctaat tgtaatagaa acattggaca
     1981 aaatagtggc aacattaatc atttgatttc taaagaatta agagataaat atgaatggaa
     2041 ccctagttta ctatctaaaa ttgacaacta ccttaaacca gcaatagaga ttaaagagca
     2101 taattttagt gataattcct tgaatattaa cgttactttt aatgcgttat acataattaa
     2161 aataggaaaa aaataacacc ttgatatgta ttgcaaaatt taatttgcat ttgttggtaa
     2221 ttttgacaac gtattaaaaa caaatgagaa agacaatggc tgaaaaattt aagataataa
     2281 tgacagaagc attgtcttta tatatttggg ggtgcaacat tttgaatact gagaaattag
     2341 aaacattgct tggcttctat aaacaatata aagcattatc tgaatatatt gataaaaaat
     2401 ataagttgtc gctaaatgat ttagcagtct tagatttaac gatgaagcat tgcaaagatg
     2461 aaaaagtact tatgcaatca tttttaaaaa ctgcaatgga tgagctagat ttaagtagga
     2521 caaaattatt agtttctata agaagactaa ttgaaaaaga aagacttagt aaagttagat
     2581 catctaaaga tgagcgtaaa atttatattt atttaaataa tgatgatata tctaaattta
     2641 atgctttatt tgaagatgta gaacaatttt taaatattta attgaaattg agtgtcgaaa
     2701 gcatagaatt tgcttatcgg cacattttta atttatacat attttaaaac taagtaacag
     2761 tttgaagaaa tcgtagttca ataatgttaa ttgtgaaaat gtatataaac ataaaaaaat
     2821 catgtataat atatgttgtt aattaaacag ttcgaaagcg agatgacatt atgggacgta
     2881 aatggaataa cattaaagaa aaaaaggccc aaaaagataa aaacacaagt agaatatatg
     2941 cgaaatttgg taaggagatt tatgttgcag caaaatctgg tgaacccaat ccagaatcta
     3001 accaagcttt aaggttggtg cttgaacgcg ctaagacata ttcagtgccg aatcatatta
     3061 ttgaaaaagc aatagataaa gctaagggtg ctggagacga aaactttgat cacctaagat
     3121 atgaaggatt tggcccaagc ggatcaatgc taattgttga tgcgttaaca aataatgtaa
     3181 atcgtactgc ctctgatgtg cgagctgctt ttggtaaaaa cggcggtaat atgggtgtat
     3241 ctggatcagt tgcttatatg tttgatcatg tggcaacatt tggtattgaa ggaaagtctg
     3301 ttgacgaaat acttgaaaca ttaatggaac aagatgtaga tgtaaatgat gtgattgacg
     3361 ataatggatt gacaatagtc tatgctgaac cagatcaatt tgcagtcgtt caagatgcgc
     3421 ttcgtgcagc aggtgttgaa gaatttaaag ttgctgaatt tgaaatgtta cctcaaacag
     3481 atattgaact ttctgaagcg gaccaagtaa catttgaaaa attaatcgat gcattagaag
     3541 atttagaaga tgtacaaaac gtattccata atgtggattt gaaataatga aatcagcaga
     3601 acaatggatt gatgaattgc aacttgaatc acatcctgaa ggtggtttct atagagagac
     3661 aattcgagaa gtattgaaag atggacgcag agcgccgttt agtagtattt attttttact
     3721 tacagatgac aatatttcgc attttcatcg aattgatgct gatgaagtat ggtactatca
     3781 tgctggcgat tctctaacaa ttcatatgat aaatccggat ggggaatata cgactgcaac
     3841 attgggtact gatatccaaa atggagatgt attgcaatat gtagtgccta aaggaacaat
     3901 ttttgcttct tcaatcgaaa tatcaaatac ttttagttta gtaggttgta tgtgtcaacc
     3961 ggcatttgag tttaagcagt ttgaattgtt taagcaatct gaattaatta cgcaatatcc
     4021 gcatcttaaa tcagtaatcg aaaaatatgc tttaaaataa aagtgatcaa tgaagtggtt
     4081 tgaaggttgt taataaacct ttgagtcact tcatttttat atgtattctt gattgaatca
     4141 gaatagattt gatgcttcag ctgtttttaa tgaaaatagc attaaatgat tttgaaaacg
     4201 ataagagtgt gttatttata tttttgaaaa atcactttta tgaagaaatg tgtgtactat
     4261 ctaattaata ttgtctattt taagtaaatt gaagcgagtt gtagtaaaat aataataaat
     4321 attttcatat gattaacaaa aactataaaa ctgtatcatg acatgacatc atttatgtag
     4381 ttttatataa taatgacaag gagttggtga ctgtgaaagt aaagtatata gataaacgtc
     4441 actggcgtcg cctaattgat agggaataca cagaggtaaa agttaataat aataggttta
     4501 agggtattat aggcttagtc acgatgaaaa aggttcgtga tcctttagag gtgacggtag
     4561 ttggacaaaa tatcattgtc gcagatgaca attataaatg gttgcaaata ctacctgaaa
     4621 agaaacgtta tagtataact gtaatgtttg ataataaagg caatccatta gaatattatt
     4681 ttgatataaa tatcaaaaat ataacgcaaa aaggtaatgc gcgtacagta gatttatgtt
     4741 tagatgtttt agcattacca agtggtgaat atgagttggt agatgaagat gatttaatgt
     4801 ttgcattaga aagtgagcaa attacaaaaa agcaatttca tgaagcatat atgattgcac
     4861 atcaaattat ggcagagtta gaaaatgatt ttaaaggatt ccaaaagaaa atcatgtact
     4921 gctttaataa aattaatgca aaggctcaaa aaaatcatca aaagccacaa aataaaacta
     4981 atattgaaaa aagcaaacaa ataaagccta agcaatataa tcaaactaaa aatcaccaac
     5041 aacaaaagaa aaactaagta attcaagctg cagccatacc aataaaattg gtgaaattat
     5101 tcgttagtaa tgaactcatg aatgcttgta gccatgaaag ttcaataatt gaataattta
     5161 tggggggaaa ttatgaagat tgaagactat cgtttactaa taacattaga cgaaacgaaa
     5221 acgttacgta aagcggctga aattttatat atatctcaac ctgctgttac acaaagacta
     5281 aaagctattg aaaatgcttt tggagtagat atttttatca gaacaaaaaa acaattgatt
     5341 acaacaactg aaggaacaat gattattgag catgctcgtg acatgttgaa aagagagcga
     5401 ttattttttg acaaaatgca ggcacatatt ggtgaagtga atggaacaat atcaatcggg
     5461 tgttcttctt tgattggaca aaccttactt cctgaagttt tgagcctata taatgcccaa
     5521 tttcctaatg ttgaaataca agtgcaagtt ggttcaactg aacaaattaa agcaaatcat
     5581 agagattatc atgttatgat aactcgtgga aataaggtaa tgaatttagc taacacacat
     5641 ttatttaatg atgatcatta ttttattttt ccaaaaaata gacgagatga tgttacaaag
     5701 ttaccattta tagagtttca agctgatccg atttatataa atcaaataaa agaatggtat
     5761 aacgataatt tagaacaaga ttaccatgca actattacag tggatcaagt agcaacttgc
     5821 aaagaaatgt tgattagtgg tgtaggtgtt acaatcttgc cagaaattat gatgaaaaat
     5881 atcagcaaag aacaatttga gtttgaaaaa gtagaaattg ataatgaacc gctgattcgt
     5941 tcgacattta tgagttatga tccgagcatg ttgcaattgc cacaagttga ttcttttgta
     6001 aatctcatgg cgagctttgt tgaacaacca aaggcgtagt tttagactaa tttaaggttt
     6061 gtatttaatt ttaaactatt cggttaaatt gaacgtagtt ggttgctaat gcaccaacag
     6121 caaaagagcc cctaattaat aaattaaaag gggacaaagg aatacagttg gttgctaatg
     6181 caccaactgc ataagagccc ctaattaata aattaaaagg ggacaaagga atacagttgg
     6241 atgctaacgc accaactgca taagagcccc taattaataa attaaaaggg gctctaagaa
     6301 gcggggttat ggatatgttt gcagcgttat tacaaataaa gaattataaa ctctttgttg
     6361 ctaatatgtt tctactaggt atgggtattg cggttacggt cccatatctt gttctttttg
     6421 caactaaaga tttaggtatg acaacaaatc agtatggatt acttctagca tctgcagcga
     6481 ttagccagtt tacagtaaat tcaattattg ctagattttc ggatacgcat cactttaata
     6541 gaaaaattat tattattctc gcattattaa tgggtgcgct tggtttttca atatactttt
     6601 ttgtagatac aatctggtta ttcatattac tatatgcgat tttccaagga ttatttgcac
     6661 cagcaatgcc ccaactttac gcatctgcta gagaatctat caatgtttca agctctaaag
     6721 atagagctca atttgccaac acagtattac gttcaatgtt ctcattgggc tttttatttg
     6781 gtccatttat tggtgcccaa ttaatcggat taaaaggcta tgctggattg tttggtggaa
     6841 caataagtat cattttattt actttagtac ttcaagtgtt tttctataag gatttaaaca
     6901 ttaaacaccc tattagtacg caacaacatg ttgaaaaaat tgctcctaat atgtttaaag
     6961 acaaaacgct tttattacca tttattgcat ttattttatt acacattgga caatggatgt
     7021 atacgatgaa tatgccttta tttgttactg attatttaaa agaaaatgaa caacatgtcg
     7081 gttatttagc tagtttatgt gctggtttag aagtgccatt tatgatcatt cttggcgttt
     7141 tatcatctag attacagact cgaacattgt tgatttatgg agcgattttt ggtggtttat
     7201 tctacttcag cattggggta tttaaaaact tctatatgat gttagcagga caggtgtttt
     7261 tagctatttt cttagcggtt cttttaggaa ttggtattag ttatttccaa gatatcttac
     7321 cagattttcc aggatacgct tcaacactat tttctaatgc aatggttatt ggacagttag
     7381 gcggtaacct attaggtggt gctatgagtc actgggtagg tttggaaaat gtattttttg
     7441 tatcagcagc atcaatcatg ttaggtatga tacttatatt ctttactaaa aatcaaaaaa
     7501 ttacaaaaga ggatgtgata tcaacatgac aattatttta tggctactta tcatcgctgc
     7561 cttcatgtta gcatttgttg ggttgattaa gccgattatt ccttctgttt tagtattatg
     7621 ggttggcttt ttaatctatc aatttggctt tcataatcag catttatcat gggtgtttta
     7681 tgtatctatg gcattgctaa caatattaat tttatgtgcc gactttttag ctaataaata
     7741 ttttgtgaat cgcttcggtg gttctaagtt tggagagtat gcagctttaa ttggtgtggt
     7801 tattggatgt tttgttttac cgccatttgg aattattatt atacctttta ttttggtatt
     7861 catagttgaa ttaatacaag gctattcatt tgaaagagca gttaaagtaa gtataggttc
     7921 aatcgtagca tttttaacaa gtagtatagc tcaagcaatc attatgttta taatgattgt
     7981 atggttcttt atagatgctt tattgattaa ttaataaaaa gcttattgca aaatatgttt
     8041 ttcggtaact gtaatttagt gattttatca ttaacagtac caaattcgca tatgatgtaa
     8101 taagcttttt gtttataaaa atgtatgaga atgttttttc gaaatatttc tttcaatgcg
     8161 taatccaata ggcataacta taaatgaaaa tataataaat acccaattgc ttacctgaat
     8221 atatggtcca aaggcaagta atgattgtgg aataaagaat acatagaaaa atcctacaat
     8281 tagtagaatg attgctaaat aggtgaatat ccatatccaa tttgaattgt taaaacactg
     8341 cagttgtatt gttttaaaac taaaccatat aaataaaaag acaataaaga atccaacgac
     8401 tactgaaaac gggaatgaaa caaaatataa attacttcca tttttttcca tgaaaaatcc
     8461 taaaaatcct ttgagaaaac taacaatccc aattaataga acgatgtgtt gatagatata
     8521 tttaaaaata tttttaaatg tttcattagg catcgctttt agttctttta ttgcatgtgc
     8581 ttttgggtcg tgattgaaaa aatctaaggc taataaacca tgttgttctg cgcttaataa
     8641 ttgtttgagt atacggttaa taattaactc tgtatcatga gggttgacgc gaaagtcaga
     8701 gcgcatataa gtcatataat tctcgaagat ttctctatca gtattgctta atcttaatga
     8761 tttaacatta ttttcttttg ttaattgcgc agtacttttc attgttactt aagcgctcct
     8821 ttaaaaatgt ttaattccaa attaaaatgg aaatgatttt atagtattaa taag
//
LOCUS       DAKTGS010000119         8647 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.119, whole genome shotgun sequence.
ACCESSION   DAKTGS010000119 DAKTGS010000000
VERSION     DAKTGS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.119"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            76..1032
                     /locus_tag="O0286_002230"
     CDS             76..1032
                     /locus_tag="O0286_002230"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0170588.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1232..1711
                     /locus_tag="O0286_002231"
     CDS             1232..1711
                     /locus_tag="O0286_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0170589.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1726..2565
                     /gene="fakB2"
                     /locus_tag="O0286_002232"
     CDS             1726..2565
                     /gene="fakB2"
                     /locus_tag="O0286_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0170590.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2651..3184
                     /gene="msrA"
                     /locus_tag="O0286_002233"
     CDS             2651..3184
                     /gene="msrA"
                     /locus_tag="O0286_002233"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0170591.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3177..3605
                     /gene="msrB"
                     /locus_tag="O0286_002234"
     CDS             3177..3605
                     /gene="msrB"
                     /locus_tag="O0286_002234"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCY0170592.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3617..4117
                     /locus_tag="O0286_002235"
     CDS             3617..4117
                     /locus_tag="O0286_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0170593.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4117..4338
                     /locus_tag="O0286_002236"
     CDS             4117..4338
                     /locus_tag="O0286_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCY0170594.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4530..6020
                     /locus_tag="O0286_002237"
     CDS             4530..6020
                     /locus_tag="O0286_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCY0170595.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            6420..6929
                     /locus_tag="O0286_002238"
     CDS             6420..6929
                     /locus_tag="O0286_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170596.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            6941..8011
                     /locus_tag="O0286_002239"
     CDS             6941..8011
                     /locus_tag="O0286_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0170597.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            8028..8642
                     /locus_tag="O0286_002240"
     CDS             8028..8642
                     /locus_tag="O0286_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0170598.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
BASE COUNT         3228 a         1185 c         1552 g         2682 t
ORIGIN      
        1 aaatgctaca taaagttaag ttatatcaaa ctaaatatac atactataaa taatgaaaat
       61 gaggtgttat cgcatatgtt gaattcattt gatgcagcat atcacagtct ttgtgaagaa
      121 gttttagaaa taggaaatac acgaaatgat cgcacaaata caggtacgat ttcgaaattt
      181 ggtcatcaac ttcgctttga cttatctaaa ggatttccac tattaacgac aaagaaagtt
      241 tcttttaaat tagtagcaac cgaattatta tggttcatta aaggagatac aaacatccaa
      301 tacttattaa aatataataa taatatatgg aacgaatggg cttttgaaaa ttatatcaaa
      361 tcagacgagt ataaaggtcc agatatgaca gatttcgggc atcgtgcatt gagtgatcct
      421 gaatttaacg aacaatataa agaacaaatg aaacaattta agcaacgtat tcttgaagat
      481 gatacatttg cgaagcaatt cggggattta ggaaatgttt atggtaaaca atggcgagat
      541 tgggttgata aagatggtaa tcattttgat caacttaaaa cagtaattga acaaattaag
      601 cataatccag attcaaggcg acacatcgta tctgcatgga atccaacaga aattgataca
      661 atggcacttc cgccttgtca taccatgttc cagttttatg tccaagatgg taagttaagt
      721 tgccagttat accaacgtag cgcagatatc tttttaggtg tgccatttaa tatcgcaagc
      781 tacgctttat tgacacacct tattgccaaa gaatgtggac ttgaagtggg tgaatttgtg
      841 catacatttg gagatgcaca tatttattca aatcatattg atgcgattca aacacaatta
      901 gcacgtgaaa gcttcaatcc tccaacatta aaaattaaca gtgacaagtc tattttcgac
      961 ataaattatg aagatttgga aattgttgac tatgaatcac atccagcaat aaaagctcca
     1021 atagcagtgt agtcattgca tagttagcta accatataga catcaaaatg acatcatagt
     1081 attttcaagt gcaaaaaagt acttttttgt gttaaacgtt ttcataaatt atgcaaaatc
     1141 attatttcta tcacacttta tgataaaaat tgtgttaaat taaagataac ttagtaataa
     1201 aaaatgaaat gatagaagaa ggaggataat tatgacttta tccattctag ttgcacatga
     1261 cttgcaacga gtaattggtt ttgaaaatca attaccttgg cacctaccaa atgatttgaa
     1321 gcatgttaaa aaattatcaa caggtcatac tttagtaatg ggtcgtaaga catttgaatc
     1381 gattggtaaa ccactaccga atcgtcgaaa tgttgtactt acttcagata caagtttcaa
     1441 cgtagagggc gttgatgtaa ttcactctat tgaagatatt taccaactac cgggccatgt
     1501 tttcatattt ggagggcaaa cattatttga agaaatgatt gataaagtgg acgacatgta
     1561 tattactgtt attgaaggta aattccgtgg tgatacgttc tttccacctt atacatttga
     1621 agactgggaa gttgcctctt cagttgaagg taaactagat gagaaaaata caattccaca
     1681 tacctttcta catttaattc gtaaaaaata agggggaaaa cgaccatgac aaaacagatt
     1741 atagtaacag actcaacatc cgatttatct aaagaatact tagaagcaaa caacattcat
     1801 gtaattcctt taagtttaac tattgaagga gcttcatacg ttgaccaagt agatattaca
     1861 tcagaagaat ttattaatca tattgaaaat gatgaagatg taaagacaag tcagccagcc
     1921 ataggtgaat ttatatctgc ttatgaagaa ctaggaaaag atggctctga aatcataagt
     1981 attcatcttt cttcaggatt aagtggtaca tataacactg cttaccaagc aagtcaaatg
     2041 gtagatgcta atgtaactgt tattgattca aaatctattt cttttggttt agggtatcaa
     2101 atacaacacc tagtagagct tgtaaaagaa ggtgtctcaa cttctgaaat agttaaaaag
     2161 ttaaatcatt taagagaaaa cattaaatta tttgtagtta tagggcaatt gaatcaatta
     2221 attaaaggtg gcagaattag taaaacaaaa ggtttgattg gtaatcttat gaaaattaaa
     2281 ccaattggta cactagatga tggtcgctta gagcttgtgc acaatgcgag aactcaaaat
     2341 tctagtatcc aatacttgaa aaaggaaatt gctgaattta taggagatca tgaaatcaaa
     2401 tccattggtg tcgcacatgc taacgtcatt gaatatgttg ataaattgaa gaaagttttt
     2461 aatgaagctt ttcatgtgaa taattacgat ataaatgtaa ctacaccagt tatttctgca
     2521 catactggtc aaggtgcgat tggcctcgta gtccttaaga agtaaattta atcttttcag
     2581 tgttaattac ttccatttca atcctttata gactaaattt ataattagat agatagagga
     2641 ggtaattcat atgacaaaag aatatgcaac attagcagga ggatgtttct ggtgcatggt
     2701 taaaccattt acatcatatc caggcatcaa gtcagtcgta tctggttata gtggcggtca
     2761 tgttgacaac ccaacttatg aacaggtatg tacgaatcaa accggccatg tcgaagcagt
     2821 acaaattacg tttgatccag aggttacttc ctttgaaaat atattagaca tatatttcaa
     2881 aacatttgac ccaactgatg atcaagggca atttttcgat agaggcgaaa gctatcaacc
     2941 agtcattttc tatcatgatg aacatcagaa aaaggctgct gagtttaaaa agcaacaatt
     3001 aaatgaacaa ggtattttca agaaaccagt gattacacct attaaaccat ataaaaattt
     3061 ctatccagct gaagactacc atcaagatta ttacaaaaag aacccggtac attattacca
     3121 atatcaacgt ggttcaggta gaaaagcgtt tatagaatca cattggggga atcaaaatgc
     3181 ttaaaaaaga taaaagtgaa ctaacagata tagaatatat tgttacacaa gaaaacggca
     3241 ctgaaccacc atttatgaat gaatattgga atcattttgc taaaggaatt tatgtagata
     3301 aaatttctgg taaaccttta tttacatctg aagaaaagtt tcattctgaa tgtggatggc
     3361 ctagcttttc caaagcgctt gatgacgatg aaattataga attagtcgac aaatcatttg
     3421 gtatgttgag aactgaagtg cgttcagaag aatcaaatag tcatttagga catgtcttta
     3481 atgatggacc taaagaaagt ggcggcttaa gatactgtat caattccgct gcaattcaat
     3541 ttattccata tgaaaagtta gaagaattgg gttatggcga tttaatatca cattttgata
     3601 agtaggagtg gaaagtatgt ttaaaaaatt attcggaaaa ggcaaagaag ttcagaaaga
     3661 tatcgcaatt tatgcaccat taactggaga attcgtgaaa attgaagata ttccagatcc
     3721 tgtattcgca caaaaaatga tgggcgaagg ttttggtatt aatccaactg aaggagaagt
     3781 tgtgtctcca attgcaggac gtgttgacaa tgtctttcca actaagcatg cgattgggtt
     3841 aaaagcagat aacggattag aattattagt tcatatcggt ttagacacag ttcaattaga
     3901 cggtgaagga tttgaagtgt tagtgtctag tggtgacgaa gttaacgtcg gagatccatt
     3961 agtaagattc aaccttgaat atattaataa taacgctaaa tctgtaattt caccaattat
     4021 aattactaac actgatcaag cagcttcaat taatatttat gatgaaaatg ctgtgattaa
     4081 aggtgaaaca aaagtgattg atgtgacaat gaactaatga aaaactattc gttttatcaa
     4141 tttgtcatga cagttcgtgg tcgacacgac gataaaggtc gtctagcaga agagatattt
     4201 gacgatcttg ctttcccaaa acacgatgat gattttaaca tactgtctga ttatattgag
     4261 acacatggtg atttcacatt gccaatgtct gtatttgatg atttatatga agaatatacg
     4321 gaatggctaa aattttaata taatttttaa taataatagt tagaaccagg gtgatgcaat
     4381 tcgttatcct ggttttaatt taaaataaac taagttgtga ctaaaaatta atcaattata
     4441 gtgaaatatg gtgcgctatc ttgcataaat tgatatgatt aactacacag aatttaaaag
     4501 tacataatac ataataagga agtgatacaa tggatgataa gcaacacaca tcttcatccg
     4561 atgatgaacg cgctgaaatt gcaacaagca atcaagacca agaaactaat tcatcgaaac
     4621 gcgttcactt aaaacgttgg caattcatat caatattaat tggtacaatc ctaataacag
     4681 ctgtcatcac agttgttgca tatattttta taaatcaaaa aataagtggt ttaaacaaaa
     4741 ctgatcaagc aaacttaaat aaaattgaaa atgtgtataa aatcttaaat agtgattatt
     4801 acaaaaaaca ggactctgac aagttaagta aagctgcaat tgatggcatg gtcaaagaat
     4861 taaaagatcc ttattctgaa tatttaacaa aagaacaaac gaaatccttt aatgaaggtg
     4921 tttcaggtga ttttgtaggt attggtgcag aaatgcaaaa gaaaaatgat caaattatgg
     4981 ttactagtcc tatgaaggga tctccagcag aacgtgctgg cattcgtcct aaagatgtca
     5041 ttactaaagt aaatggaaaa tcaattaaag gtaaagcatt agatgaagtt gtcaaagatg
     5101 ttcgtggtaa agaaaacact gaagtcactt taactgttca acgaggtagt gaagaaaaag
     5161 acgttaagat taaacgtgaa aaaattcatg ttaaaagtgt tgagtataag aaaaaaggta
     5221 aagttggagt tattactatt aataaattcc agaatgatac atcaggtgaa ttgaaagatg
     5281 cagttctaaa agctcacaaa gatggtttga aaaagattgt tttagattta agaaataatc
     5341 caggtggact actagatgaa gctgttaaaa tggcaaatat ttttatcgat aaaggaaaaa
     5401 ctgttgttaa actagaaaaa ggtaaagata ctgaagcaat tcaaacttct aatgatgcgt
     5461 taaaagaagc gaaagacatg gatatatcca tcttagtgaa tgaaggttca gctagcgctt
     5521 ctgaagtgtt tactggtgcg ctaaaagact ataataaagc taaagtttat gggtcaaaaa
     5581 cattcggcaa aggtgtcgta caaactacaa gagagtttaa ggatggttca ttgttaaaat
     5641 atactgaaat gaaatggtta acgccagatg gtcattatat tcacggtaaa ggcattaaac
     5701 cagacgttac tattgacaca cctaaatatc aatctttaaa tgtcattcct aatacgaaaa
     5761 catttaaagt tggagacgat gataaaaata ttaaaactat taaaattggt ttatcagctt
     5821 taggttataa agttgataat gaatcaacgc aatttgataa agctttagaa aatcaagtta
     5881 aagctttcca acaagcgaat aaacttgagg taactggtga atttaataaa gaaacgaata
     5941 ataaatttac tgagttatta gttgaaaaag ctaataaaca tgatgatgtt ctcgataagt
     6001 tgattaatat tttaaaataa gcgatacaca ctactaaaat tgtattatta ttatgttaat
     6061 gacacgcctc ctaaatttgc aaagatagca atttaggagg cgtgtttatt tttattgacg
     6121 tctaactcta aaagatataa attagacatt tacaaatgat gtaaataacg caatttctat
     6181 catcgctgat aacaattcat ggtttaatat gcaatgagca tatacttttt aaatagtatt
     6241 attcactagt tttaacaatc aattaattgg tatatgatac ttttattggt tatttttatc
     6301 ccatagtgtg ataattacta tttttcattc ataataaagg tttaaagcat gttaatagtg
     6361 tgtaagatta acatgtactg aaaaacatgt ttaacataat gatataagga gtgacgtaca
     6421 tgatccgtct aggtaaaatg tcagatttag atcaaatctt aaatctagta gaagaagcaa
     6481 aagaattaat gaaagaacac gacaacgagc aatgggacga tcagtaccca cttttagaac
     6541 attttgaaga agatattgct aaagattatt tgtacgtatt agaggaaaat gacaaaattt
     6601 atggctttat tgttgtcgac caagaccaag cagaatggta tgatgacatt gactggccag
     6661 taaatagaga aggcgccttt gttattcatc gattaactgg ttcgaaagaa tataaaggag
     6721 ctgctacaga attattcaat tatgttattg atgtagttaa agcacgtggt gcagaagtta
     6781 ttttaacgga cacctttgcg ttaaacaaac ctgcacaagg tttatttgcc aaatttggat
     6841 ttcataaggt cggtgaacaa ttaatggaat atccgccata tgataaaggt gaaccatttt
     6901 atgcatatta taaaaattta aaagaataga ggtaatatta atgacgaaaa tcgcatttac
     6961 cggaggggga acagttggac acgtatcagt aaatttaagt ttaattccaa ctgcattatc
     7021 acaaggttat gaagcgcttt atattggttc taaaaatggt attgaaagag aaatgattga
     7081 atcacaacta ccagaaatta agtattatcc tatttcgagt ggtaaattaa gaagatatat
     7141 ttctttagaa aatgccaaag acgtatttaa agtattgaaa ggtattcttg atgctcgtaa
     7201 agttttgaaa aaagaaaaac ctgatctatt attttcaaaa ggtggatttg tatctgtgcc
     7261 tgttgttatt gcagccaaat cattaaatat accaactctt attcatgaat ctgacttaac
     7321 accaggatta gcgaataaga tagcacttaa atttgccaag aaaatatata caacatttga
     7381 agaaacgcta aactacttac ctaaagagaa agctgatttt attggagcaa caattcgaga
     7441 agatttaaaa aatggtaatg cacataatgg ttatcaatta acaggcttta atgaaaataa
     7501 aaaagtttta ctcgttatgg gtggaagctt aggaagtaaa aaattaaata gcattattcg
     7561 cgaaaactta gatgcattat tacaacaata tcaagtgata catttaactg gtaaaggatt
     7621 aaaagatgct caagttaaaa aatcaggata tatacaatat gaatttgtta aagaggattt
     7681 aacagattta ttagcaatta cggatacagt aataagtaga gctggatcaa atgcgattta
     7741 tgagttctta acattacgta taccaatgtt attagtacca ttaggtttag atcaatcccg
     7801 aggcgaccaa attgacaatg caaatcattt tgctgataaa ggatatgcta aagcgattga
     7861 tgaagaacaa ttaacagcac aaattttatt acaagaacta aatgaaatgg aacaggaaag
     7921 aactcgaatt atcaataata tgaaatcgta tgaacaaagt tatacgaaag aagctttatt
     7981 tgataagatg attaaagacg cattgaatta atggggggta atgctttatg agtcaatgga
     8041 aacgtatctc tttgctcatc gtttttacat tggtttttgg aattatcgcg tttttccacg
     8101 aatcaagact tgggaaatgg attgataatg aagtttatga gtttgtatat tcatcagaga
     8161 gctttattac gacatctatc atgcttgggg ctactaaagt aggtgaagtc tgggcaatgt
     8221 tatgtatttc attacttctt gtggcatatc tcatgttaaa gcgccacaaa attgaagcat
     8281 tattttttgc attaacaatg gcattatctg gaattttgaa tccagcatta aaaaatatat
     8341 tcgatagaga aagacctaca ttgctgcgtt taattgatat aacaggattt agttttccta
     8401 gcggtcatgc tatgggatca actgcatatt ttggaagtgg tatctatcta ttaaatcgat
     8461 taaatcaagg taattcaaaa ggtattctta tagggttatg tgcagctatg attttattga
     8521 tttccatatc acgtgtatat ctaggtgtac attatccaac agatattatt gccggcatta
     8581 ttggtggatt attttgcatt attttatcaa cgttattact tagaaataaa ttaataaatt
     8641 aaatagt
//
LOCUS       DAKTGS010000120         8640 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.120, whole genome shotgun sequence.
ACCESSION   DAKTGS010000120 DAKTGS010000000
VERSION     DAKTGS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.120"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(306..506)
                     /locus_tag="O0286_002241"
     CDS             complement(306..506)
                     /locus_tag="O0286_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0170599.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(863..1549)
                     /locus_tag="O0286_002242"
     CDS             complement(863..1549)
                     /locus_tag="O0286_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0170600.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
     gene            complement(1890..2360)
                     /locus_tag="O0286_002243"
     CDS             complement(1890..2360)
                     /locus_tag="O0286_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170601.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
     gene            complement(2695..3717)
                     /gene="emp"
                     /locus_tag="O0286_002244"
     CDS             complement(2695..3717)
                     /gene="emp"
                     /locus_tag="O0286_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCY0170602.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            complement(4068..5594)
                     /gene="vwb"
                     /locus_tag="O0286_002245"
     CDS             complement(4068..5594)
                     /gene="vwb"
                     /locus_tag="O0286_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HCY0170603.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            complement(5815..8616)
                     /gene="clfA"
                     /locus_tag="O0286_002246"
     CDS             complement(5815..8616)
                     /gene="clfA"
                     /locus_tag="O0286_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCY0170604.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSE
                     SDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLL
                     ASIGSLLLFRRKKENKDKK"
BASE COUNT         2451 a         1544 c         1291 g         3354 t
ORIGIN      
        1 tattgttaaa cattttgagt gtttaacatt acttcttgtt atcgccatat acagacttta
       61 attctcgtca ttattaaagt tctgtattgg cgctttttta tattcaaaat caaaagtcgg
      121 acagatgaaa agtaaaaaac ttttccatca gttcgattta ttttagaacc attatatatt
      181 gttaaatctc caactaaatt tattggacaa aaaaataacg gctagtgctt ttaccacacc
      241 aactgttatc gatttaaagc ataaaaaaac agccctatag taaggactgt ttgaataagt
      301 taaaattaca ttttaactac gtttgcagct tgctcgccac ggtcgccttc aacgatgtcg
      361 aattcaactt tttggccttc ttctaatgat ttgtatccat cttcagcgat tgctgagaag
      421 tgtacgaata cgtcgctacc atcttctctt tcgatgaaac caaaaccttt ttctgcatta
      481 aaccatttaa ctgtaccgtt attcatattg aatacctccg tcgtgctttt gcactaaata
      541 ttttgtaaca aattcataaa tgaaaaggag aatattctat ataaataact cacatttttc
      601 ttcacgctct ttattactta actttcatta tacactttta aatatagaag taaagcatta
      661 ttttattttc tgtgataata cacattgaac tatatagtaa cattccattt taatactaaa
      721 tttaaaattt ttattttcta atattaaata cacttacttt tgatactatt tactttttta
      781 attctgaatt gataataaat catatcgtga tctaaaaatt ataaaagtgc cactagcagc
      841 agtgacactt ttacaatgag cattattgac ctgaatcagc gttgtcttcg ctccaaatat
      901 ttaatttctc tttttttgct tgtgcttcac tttttcttaa aagttgttca tgtgtattgt
      961 taggtttata aacataagca actttagcca agccttgacg aactaaagct tcgtttacca
     1021 tttttccatc agcataaata tacgctaagc cacgtccata tttatcagtt ctttgacctt
     1081 tgtcaaactc gacttcaatt ttctttgcat tttctaccat ttttttcgta aatgcacttg
     1141 cttcaggacc atatttctct acaccttttt taggatgctt tgtttcaggt gtatcaacca
     1201 ataatagtct gaatgtcatt ggttgacctt tgtacattaa tttaaccgta tcaccatcaa
     1261 tcgctttaat taatgtcgca ggttctttat gtaatttttt agttgaagtt gcactatata
     1321 ctgttggatc ttcagaacca cttctattta cgccattatc tgtttgtgat gcatttgctg
     1381 agctacttag acttgaaact acaactaaag ttaacactaa acaactagta gcgaaataga
     1441 aaaacctctt tgcgtattgc cctttcgaaa cattactgat agccatccct ataagtaata
     1501 ttgaaacgat tgccatacat atgccagcac ttaataagta ttctgtcata actaacacct
     1561 ctttcttttt agttaatttt aatattaaac gttaattaat tataattcta catttttaca
     1621 attaattaat aaaaatttaa taattataat aaaataacta tacttacttt tatgtatttt
     1681 ctatatttta ggtgccagaa cttatatatt tagccttatt tgtaaaatta aaaagcctac
     1741 aacatgaatc gttgcagact atcatatcga gacaaagagg tttattagtg tctctatatc
     1801 tatgtggaat ttgaaaagat tatttataat atttacgggg ataaattgtc atttaaaaac
     1861 gagatgtata acttataatt tactttggat tagcgttgta atccattata aaccttatca
     1921 atttgtttat tgaaatagtt ttgatgcggt ttgcttaatg tgttaacttt ttgtttggct
     1981 ttcatcctag catcagcact ttgtgtatct ttatagttct ctactaagct ttgtgcttct
     2041 ttaacatgat cacgtctaat aacgcgtttg tcacggtctg tgaaatgtga tgagtcaatt
     2101 tcactataac caaccatagc tcctgattta ccgcccattg ttaatatgtt gaactccatc
     2161 aagtttgaat tgctattaga ttgaaaaata ttaatttgag catgttcaac tgttataact
     2221 ttctcttccg atacaatttc agtaaattgc ttagtatcat ttgcgccact ctctgttact
     2281 aatgcgtttg cgtgattaga atgccataat tgagttgcac aaattacacc cattgttagt
     2341 actaatactt tccttttcat ttgtttatgc tcccttattt gctttgatat aaaaaccgac
     2401 tttttcaccc tgttttcttc gccattgaaa attatatacc aatattttag aaacaataat
     2461 taacttgcga tgaacaaact attaacaata atcttgagta ttatatttat cttaattaat
     2521 aatatattta tttaagatta tgttacttcc aactttcaaa gtagaaaaac ggtataattt
     2581 gttgatgggt gtttattgat aaactgcaaa aaatacagcc tacaaccaca tagattgtag
     2641 actatattta aaataatagg tatttatcat atctcgtaaa cttagtgcca atttttatac
     2701 tcgtggtgct ggtaagctac ccttaaattc aggaacgtag tgtgtagggc tataacttgg
     2761 aacagcatat tgataattta catttttgat atttaatgat ggtttcccaa ttttataacc
     2821 atttgattgt gaaaatgaga aatatttctt cacaccttta actactttat aagaatagaa
     2881 gtatttatag tcatatgctt tatttacttt agcattttga tgtgttgctg ttgtgttatt
     2941 ttggaaactt ggtacatgca tacgatgtga attgtgacca tatggtggaa ttaccttgaa
     3001 actatttatt tgtggcacaa cacaaaagtg attaatttta atgctagcat gccctggtgt
     3061 aacaaattta tgcgcgtgat atccaggaac tgcaaaatga tgcttgataa ttaaagattg
     3121 agatggatgt gtatatctag gcgattctga tggtttaaca ataaagtgtt tattaataga
     3181 atcctttgca tgatttacat gtttatgtac atgtgttgat ttgtatgaag taataacttt
     3241 cttgtagtgg gtttgcgtag taatgaagtg gtggtttact ttgttttgcg taataaatct
     3301 atttacttct gggcctttag caacaaaatg cttatcaact tgttgtgaag ctggattgtt
     3361 tgttgctaca ttcacgcgat tattgatttc tttgtactct ggcacaatgt ttcctaactt
     3421 tgattctggt acgacaaagt ttttatctac aattttacta gctgccgatg aatctgcaat
     3481 cttattcaat ttatcttcag aagctacaaa gtttttattg tcagttaaat tactaacatt
     3541 tacttttggc gaattcttaa attcatcgta agctggaata attttattaa ttcctgattc
     3601 tggaactaca aattttttgt caacactctc tgtcactgat gctttagcgt gatttgaatt
     3661 cataatttgt gtagcaaata gcgtgctcat agttaaaact aataatttct ttttcatctg
     3721 ttatttctcc tttatataga ctcaatatta taaccaatat aatttccctg ttatattcac
     3781 taacagcatt atataccaga attttcagta taataattaa cttgaagtaa acgttgtctt
     3841 aacattttta ttgtttttca gcttaaaatt aattattgat attgatagtt aagcataata
     3901 attttttcgt aatataaagt gaaaaaagta atagtccaca cctgtttaga atgtggacta
     3961 tactagattg catcattgaa atgatgactt tgatattatt tattgctagt ttaaaatgtt
     4021 gttattttta cgacaaactc attatttgct ttgcagccat gcattaatta tttgccatta
     4081 tatactttat tgatttgttt attaaaacgt ttttgatgac cttcacttaa agtatttact
     4141 ttttgttgtg catttattcg gtctttataa ctatgtgtat ctttataatt atcaactaac
     4201 ttttgagctt ctctaacgtg ttcacgctta attacacgct tctcacgttc agttagatga
     4261 gaagaatcaa tttgacttat accaaccagt gatcctgatt ttctaccagg taaagcagac
     4321 gtttcttcaa attcaacaac attattatgc gtgtgatttg aagttgttgg tgattgatgc
     4381 gttgttgtga aatcatgact ttcaccattt aatccagcca attgttttgg tgcattataa
     4441 ataatttgtt gctgagtttg acgctcaact gtaggcattg gtacctgtgt cgttgtttca
     4501 gtatatgtag tcggtgatgt tggtttcttt gttggtgcag aaaccacaag tttcttgtcg
     4561 ttttcgttgt caatacgttg tttatgctca aaatcatatt ctaatgacac tacaggtgtt
     4621 ttcttaattt tttgattatc caaaaatctc gcttttcttt cttctgctct tttatcatat
     4681 tcagcttttt gttgttcaga aacttcttga gatgcaggtt tgtgattttg agtatttaaa
     4741 cttcttttac ttctcttgct tctttttgat tcatcacttt ttgctgcttc agtgtcagat
     4801 tttaattgag ccatattttt atgatttttc tcttcttgtt tttcatcttc taaaacagga
     4861 atattattag gtcttgtttt atctatatca ctaaaaaatt catcaattat ggtttctaag
     4921 tcttcttttt tattttctaa catccttttg ttaactgctt ttttatttgc tctttcttca
     4981 tctttatatc ccataattaa atctaactta ctatataaac tttcaacatc atctctatag
     5041 ttttgataaa atgttttacc taacattaat atctgatttt ctaatttgtt taattctaag
     5101 tcgcacttta attgctcctc ttcattaaag tctttcaatt cagggttatc tttttttata
     5161 tcttcaattt cttttaagaa ctcctcactt tgttttttca aagtattaaa tacactttca
     5221 tatctttcat gagataatcc tagataatct aaattatcag tatttccatt ttttattttt
     5281 ttctcttcac taaaaaattt cttcaaagcc tcatcttcag ccataaatct ctgttgatat
     5341 tttttcatag cttctttata ttcaggatta tcaaatcttt cattatctaa gtttggaaag
     5401 gaccatatta aatcatccaa gcttttctta tactcttcta ctgttctaga tttattttta
     5461 ttattagtca gtttcaacga ctcagataca tatggattct tctccccaga aaccactgca
     5521 ctcgcacgat tactttccca aatttgtgat acacataatg ctcccaatga taaaactagc
     5581 aatttatttt tcaactgtat tttctcctta attttcccta attaacaacc taccttacac
     5641 cctatttttt cgccaagcca aattatataa catctacctt tttaaaataa ttaactgttg
     5701 atgaattaat tattaattat attcaaacaa tatatttgga aaatccaagt aaaaaagcca
     5761 ccttttaagg tggcttcttc atgaatcata tgattaattt aatatcatta ttacttattt
     5821 cttatcttta ttttcttttt ttcttctgaa aagtagtaat gaacctattg atgctaataa
     5881 tccccaaatt agtgacgtat ttgcttcatc ttcagaacct gtatctggta atggttcttt
     5941 actatcttta gcctcatttt tattagaagc attagtacca tttttaggtg aattaggcgg
     6001 aactacatta ttgttagaac ctgactcgga atcgctattt gaatcacttt ctgagtcgga
     6061 atcacttgat gaatcggagt cactacctga gtctgaatca ctcgctgaat ccgagtcact
     6121 atctgaatct gagtcactgt ctgagtcgct gtctgaatct gaatcgctgt ctgattctga
     6181 atcgctgtct gattctgaat cgctatctga gtccgaatcg ctatctgaat ccgagtcgct
     6241 atccgagtct gagtcgctat ctgaatccga gtcactgtcg gagtcggaat cactatctga
     6301 atccgaatca ctcgctgaat ccgaatcact gtctgagtct gagtcactgt cggaatctga
     6361 gtcactgtcg gaatctgaat cgctatctga atccgagtca ctgtcggaat ctgagtcact
     6421 gtcggaatct gaatcgctat ctgaatctga atcgctgtct gaatctgaat cgctgtctga
     6481 atctgagtcg ctatctgagt cggaatcgct atctgagtcg gaatcactgt cggaatctga
     6541 atcgctgtct gagtcggaat cactgtcgga gtcagaatcg ctgtctgagt cggaatcact
     6601 gtcggaatct gaatcgctat ctgaatctga atcgctatct gaatctgagt cactgtctga
     6661 gtcagaatcg ctatctgaat ctgagtcact gtcggaatct gagtcactgt ctgagtcaga
     6721 atcgctatct gaatccgagt cattgtctga gtcggaatcg ctcgctgagt cggaatcgct
     6781 tgctgaatct gaatcactcg ctgagtctga atcacttgct gaatctgaat cactcgctga
     6841 atctgaacca ctatctgatg tagaatcact acccgaatct gaaccgctat ctgaattaga
     6901 atcgctgcca gaatctgaac ctgggtcaga atctgaatcc tctggaattg gttcaatttc
     6961 accaggctca tcaggttgtt caggaacaac tggtttatcg ataccgtcac cagaacctga
     7021 tccgttatta aatgctactt cgttgtccca tgacatagag cgccaaatta tattcgagtt
     7081 atacccatat aaagttgaac gtaaagctaa atcacctttg ctattcggat caatatgacc
     7141 attaacaact actatatacg gtgttgtaat ttgatcatca ggcgtattaa actctacttt
     7201 atattgattt ggatttggga atgtaatatt cacactatta gtgacatcct caaagttttc
     7261 tggattcaca aagtaacttt cagataaatc agctgcatta tctactttat atactttaat
     7321 acttgtattt tgctgatcta ttaatgcatt actatccgta tttggtttta aattacctgt
     7381 taaaaccggc gcaataacgt tatctccact tggattgaca taaattgtct gacgatacgt
     7441 attatttgtt ttatcgattt ggtcaattgt acctttaata gataagttat aaaacttacc
     7501 atatttttca taatctacta atactgtttt gtttgctgtt gtactaccta tgccagtagc
     7561 caatgtcaca ttacctgtct ttttaacatt ttcagggtca atataagcgg gcatggtcaa
     7621 agttgctttt acatcatctt tagtatttac atagtctgta aatgtataaa taacattacc
     7681 atcactatcg attacaccat ttgccaatac ttgatctcca gccataattg gtggcacttt
     7741 agcagttgaa gttacaccat ttaagtttaa ttctttaggt acagttattt tgaatgtgtc
     7801 acctttaaca gcagaattag gcactgaaaa accataattc agtttgacat aacctgcttg
     7861 gtgcggatac acagtcgtac cagagtcaat accaactgtc acattcgtca actgattcgt
     7921 aatatctgtg ccagctgccg gtgcatctgc agctactgcc gctaaactaa atgctctcat
     7981 tctaggcgca cttgtattaa ccgcttgatt aactacatct ttattacttg catctgtact
     8041 ctgtggagct gattcattgt ttgaaggtgt tgcttcagtt gaagtatctt gcgttgttga
     8101 aacattttcc gcatttgtag aattttgagg tgaatttaca gatgatactg tattagtatc
     8161 attagaagtc gtttcattac ttgtttgatt cactaattcc tccgcatttg tattgcttga
     8221 ttgagttgtt gccggtgtat tagcttgatt cgttgtcgta gtagtagctt caccagttac
     8281 cggcgtttct tccgtagttg catttgttga tgatgattgt gtcgtttcct gttgtgctgg
     8341 attttgcgcc acactcgttt cgccattatt agtgtttgac gatgttttag tatcactcac
     8401 gtttgtgtcg tctgttttag gtgcagcact aacgctactt gaatcattac ttttgctttc
     8461 gttacttgcg ctatcagatt gcgtaacact attttcactt gcatctgctt ctttactgct
     8521 gagtagtcca aaaccgatta acgtacctac aagcactgaa gccacgccaa tcgatttttt
     8581 ccgaattgcg tgtttttctt ttttcttcat attcatttta ttccctcttt ttaaaaagtc
//
LOCUS       DAKTGS010000121         8568 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.121, whole genome shotgun sequence.
ACCESSION   DAKTGS010000121 DAKTGS010000000
VERSION     DAKTGS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.121"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(11..1372)
                     /locus_tag="O0286_002247"
     CDS             complement(11..1372)
                     /locus_tag="O0286_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0170605.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1720..2841
                     /locus_tag="O0286_002248"
     CDS             1720..2841
                     /locus_tag="O0286_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCY0170606.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2890..4158
                     /gene="icd"
                     /locus_tag="O0286_002249"
     CDS             2890..4158
                     /gene="icd"
                     /locus_tag="O0286_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCY0170607.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4548..5252
                     /locus_tag="O0286_002250"
     CDS             4548..5252
                     /locus_tag="O0286_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0170608.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5252..6913
                     /locus_tag="O0286_002251"
     CDS             5252..6913
                     /locus_tag="O0286_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCY0170609.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            7412..>8568
                     /locus_tag="O0286_002252"
     CDS             7412..>8568
                     /locus_tag="O0286_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170610.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHV"
BASE COUNT         3039 a         1203 c         1555 g         2771 t
ORIGIN      
        1 aaacttgttg ttatttattt gatttagcag cgatacgttt atatcttaag tacataaatg
       61 ctaaaagtat aaaccaaatc ggaataaaat aaattgcacg tcttgtatca acattaataa
      121 ataataaccc gaacacaaaa atgaagaata caaatattaa gtagcccata tatttgccac
      181 ctaatagttt gtacgtagca tttttatgta gatctgggtt tttacgacta taattgatat
      241 atgcaatgat aatcagaccc catacaacta aaaataacac tgtagagatg gtagtcacat
      301 acgtaaatac ttttgtcgca tctgggaaaa tatagtttag taatgctgcc acaagtaata
      361 atgctgatga agcaaagatt gcaacatgtg gaacgccata tttattcgtc ttagaaaagt
      421 tcggaggtgc ttgttgttga cttgataaac cgaaaagcat acggctattt gagaatatac
      481 cactgttaca tgatgaagca gcagcggtta atactacaaa attaatcaag cccgcagcaa
      541 acggaattcc gatcaatgcg aataatttta cgaatggact gttatcagga tcaacttgct
      601 gccaagggat aatagacatg ataaccgcta acgccccaac gtaaaatatt aaaattctaa
      661 tgggtacact gttaattgct tttggaattg ttttaactgg atcttttgtt tccccagctg
      721 taacaccaat catttcaatt cctacaaatg agaatagtgc catttggaaa gacataaaga
      781 atccagaagc acctttagcg aatatgccgt gttcatatag atttgtgaaa ctggcatgcc
      841 cgaattgagt tttaaatgca aatagaatca tgacgaaacc aactactatt aaaccaataa
      901 ttgtcgctat tttaatgata gagaaccaaa attctaattc tccgaaaagt cttgcgctaa
      961 gtaggttgaa tgacattaat aacagtacac aaaatagtgc acttatccag tttggaattt
     1021 ctgggaacca aaagctaaca tattttgcca cagccgttac ttcagccata cctgtaataa
     1081 tccaacagaa ccagtatgtc catccggtaa caaatcctgc aaaaggccca atatatgtat
     1141 tggttacatc tgcgaaagat ttaaattcag tattctgtat aatgatttct cctaaacctc
     1201 gcataaacat aaataacata aatcctataa tgatgtatgt taatagaatt gaagggccgg
     1261 ttaatgcaat cgtttgacca gcacctaaga ataagcctgt accaattgca ccgccaattg
     1321 caattaattg tatgtggcga ttgctcagtt ccctttgtaa tttttcagcc ataatacatc
     1381 tcccttaaat atagatatgt ttattatgca cttatattga gatatataca attattttcg
     1441 gtaaaaatgt gtaaaattcc atgttaatat actttggttt ttataatcat atataataac
     1501 caattgaaaa tttaattcta ttgtaaaatt catggattat tcacatcttg aaaaagcttt
     1561 aatggtgcta tttgtggcta ttctgtgaca tttacataga tttacaaaaa aattgttgca
     1621 catataatgc cagtttttat atttcacaaa cgaaatgcgt ttagctataa tattagttga
     1681 aagccatttc ataaagaaac agtaaagggg aaatttatca tggcagaatt acaaagaggt
     1741 ttagaagggg ttatcgcagc ggagactaaa ataagttcaa ttattgaaag tcaattgact
     1801 tatgccggct atgatattga tgatctagct gaaaatgcgc aatttgaaga agttattttc
     1861 ctattatgga actatagatt gccaaacgaa gaagagcttg ctcatctaaa agggaaatta
     1921 aatcaataca tgacattaaa tcctcgtgtg tatacacatt ttgaggagta tgttacagat
     1981 cacgtgcatc caatgacagc attacgtacg tcattatcat atattgcaca tttcgatcct
     2041 gatgctgaaa atgaatcaga tgaaaatcgt tatgaaagag caatgcgtat acaggctaaa
     2101 gtagcatcat tagttacagc gtttgctcga gtaagacaag ataaagaacc acttaagcct
     2161 aatcctgact taagttatgc ggcaaacttc ctatatatgt tacgtgggga attaccaaca
     2221 gatatagaag tagaagcctt caataaagca cttattttac acgctgatca tgagttgaac
     2281 gcatctgcat ttacggcacg ttgtgcggta tcatcattgt cagatatgta ctcaggtatt
     2341 gtagcagccg taggttctct gaaagggcca ttacatggtg gtgcaaacga acaagttatg
     2401 acgatgttat ctgagattgg gtcaattgaa aatgttgatg cttacttaga tgaaaaattt
     2461 gctaataaag ataaagtaat gggcttcggt catcgtgtat ataaagatgg tgatcctaga
     2521 gcgaaatatt taagagaaat gagccgtcaa attacgaaag acgctggtcg tgaagaatta
     2581 tttgaaatgt cagtgaaaat ggaaaaacgt atggcagaag aaaaaggatt aattcctaat
     2641 gttgattttt atagtgcgag tgtttatcac tgtatggaaa tacctcatga cttattcacg
     2701 ccaatctttg ctgtaagtcg ttctgcagga tggattgctc atattttaga acaatataaa
     2761 gataatagaa ttatgcgtcc tagagcgaaa tatattggcg aaacgaatcg taagtatatc
     2821 ccgcttgaag aaagaaaata atcaatacaa attaaaaatg aagatgtaaa atttggaggt
     2881 aaaataacta tgactgcaga aaaaattact caaggaactg aaggattaaa cgtacctaat
     2941 gaaccaatta tcccatttat tatcggtgat ggaattggac cggatatttg gaaggcagca
     3001 agccgagtta tagatgctgc tgttgagaaa gcctataatg gcgaaaaacg cattgaatgg
     3061 aaagaagtgc tagctggcca aaaagcattt gatacaactg gtgaatggtt acctcaagaa
     3121 acacttgata caattaaaga atatttaatt gctgttaaag gacctttaac aacaccaatt
     3181 ggtggtggta ttagatcatt aaatgtggct ttacgccaag aattagattt atttacttgc
     3241 ttaagaccgg tacgttggtt taaaggagta ccatcacctg ttaaacgtcc acaagatgtt
     3301 gatatggtta ttttccgtga aaatactgaa gacatttatg ctggtattga atttaaagaa
     3361 ggtacaacag aagttaaaaa ggtaattgac ttcttacaaa acgaaatggg tgcgacaaac
     3421 attcgattcc cagaaacttc aggtattggt attaaaccag tttctaaaga aggaactgag
     3481 cgattagtta gagcagctat acaatatgct atcgataata accgtaaatc agttacttta
     3541 gttcataaag gtaatattat gaaatttaca gaaggctcat ttaagcagtg gggttacgat
     3601 ttagcattat ctgaatttgg tgatcaagta ttcacttggc aacaatatga cgaaattgtt
     3661 gaaaatgaag gcagagatgc tgctaatgct gctcaagaaa aagctgaaaa agaaggcaag
     3721 attatcatta aagattctat tgctgacatt ttcttacaac aaattttaac tcgtccagct
     3781 gagcatgatg ttgtagcaac tatgaacttg aatggtgact atatttcaga tgctttagct
     3841 gcacaagttg gtggtattgg tattgcgcca ggtgcaaaca ttaattatga aacaggtcat
     3901 gctatttttg aagcaacaca tggtacagct ccaaaatatg caggtttaaa taaagtgaat
     3961 ccatcttcag taattttaag ttctgtatta atgttagaac atttaggatg gcaagaagcg
     4021 gcagataaga ttacagattc aattgaagat acaattgctt caaaagttgt tacttatgac
     4081 tttgcccgtt taatggatgg tgctgaagaa gtttctacat cagcatttgc agatgaattg
     4141 attaaaaatt taaaataagc aaaataaaat taggtgagtc tagcaacttt aattgctagg
     4201 ttcgcctttt taaatgaatt gaagttggca acaggataga aataataaag agtcaccaat
     4261 gattgattat gtagtgtttc ttacacatta gacaaagcga atatgtacat aagcttagga
     4321 ataaatgagt aaaactcatg cataggtagt gggatagaaa tgctattttt acaaaattta
     4381 tttcgttgtc ccagagtaag tcatgcaaat gatagaagga aatataagaa gtattttatt
     4441 tatatgtaaa gtaatgtaaa aaatgtgtaa agataatgtg taggatgtta tttttataaa
     4501 tagttcggtt ataatgtaaa tataattata agttagggag gcataccatg tcgcaaaaag
     4561 tgttggtagt agatgacgaa cattcaatcg taacattact taaatataac ttagaaacag
     4621 ctggttatga agttgttgtc gcatttgatg gtgatgaggc tttagaaaag gtagaaagtg
     4681 aacagccaga tttaattatt ttagatgtta tgctacctaa aaaagatggc attgacgtat
     4741 gtaagactgt aagaacaaat aaaaatttag tccctatatt aatgttaacg gcgaaaaatg
     4801 atgaatttga tcgggtatta ggtttagaat taggtgccga tgactatatg actaagcctt
     4861 tttcacctag agaagttgtt gcccgtgtga aagccatttt aagacgttct caatttgtaa
     4921 aagaagtaga agagattgac gatgatatta ccatcggatc gatacgtatt agacccgatt
     4981 attttgaagt atataagcat aatgagttat tggagctaac accgaaagaa tttgaactct
     5041 tgctttattt gatagaaaga caaggcagag tcattacgag agagcatatg ttaaatacgg
     5101 tatggaatta tgaatttgca ggagactcaa gaattgttga tgtacatatc agtcatttaa
     5161 gagataaatt agaagataat cctaagaagc cacaacttat taaaactgtg cgtggcttag
     5221 gctataaatt ggaaagacct aaagaacaat gatgaagttt caccaccgct taatgttatt
     5281 gataagcagt atattaatta tcagttttgt tacattaggc attattattc acgcttccat
     5341 cacatatgtt ttatcagaaa aacacgaaca agctctagca acagatgcaa gatcatatat
     5401 ttatttagtc caagataatg agattgataa agttaaaaaa attgtaaaac agcaaaatat
     5461 agatttattg ataactaagc atgataaaaa ggtgtttagt agtggcaaac tgaaagctat
     5521 cccaaatagt gatagctata ttcaaaaaag ggacgttttt atttttaata agaaggttaa
     5581 cggttatcat gtatggatta aaggttacaa taacgatatt acagaaatgc attggacgtt
     5641 gtggaaatat cttattttga catgtttagt tgtactcatt tgtctgtatt ttgcaagccg
     5701 tagttttaag cgtacattaa tccgtcccat tcaagaagtt acttatgcaa cacaattgtt
     5761 ggcaaatggt tattatcaca tacgtgtacc tgaaagtaat gttgtagaaa ctaaagcgct
     5821 ttttgtgtct accaatgatt tagcacgacg ccttcaaaag cttaataatg agcaaaaaat
     5881 tcaatccaac cgtttaaaaa caacgattga aaatattcca agtgctattt taatgataga
     5941 tcgaaatgga aaaatcgttg ttgcaaataa agcgtattat gaacagttca acatctcgca
     6001 taatattgaa caagtaggct atcacggcta tgttaatact gaaattgagc aattaatttt
     6061 agaaagtttt aaagttgaaa aacctattta tgaacaattg gaagttgcaa taaaccaagt
     6121 ccatgccaag tactttgata tttcatgcgt tccgatttta actaggtctc aaaaaagttt
     6181 gcaagggatt ttagttgtga tgcatgatat aacaaatctg aaacaacttg aaaatctaag
     6241 acgtgaattt gtagcaaatg tttcacatga attaaaaaca cctattactt caattaaggg
     6301 gtttgctgaa acgcttattg atggtgcaaa aaatgatgcg gaatcattag acatgttttt
     6361 aaacataatt ttaaaagaat caaatcgaat agaatcttta gtaacagatc tgttagattt
     6421 atcgcatatt gagcaacata cagagttaga tacagactat atgaatttat cagatttaac
     6481 gcggcgtatt attgataata tgatgactca agccaatcaa aaaaatattt ccattcatac
     6541 tgatattgaa aaagatgtca ttgttaaagc acaagaaagt aaaattgctc aagttatcac
     6601 gaatttgtta acgaatgcaa ttaactattc ttatgaagat ggagatatta acgttcgtgt
     6661 gtatcgagat gactttcgtg tcattttcga agtacaagat tttggtatag gtattaaatt
     6721 agaagaccaa caacgtattt ttgaaagatt ttatagagta gataaagcac gaagtagaga
     6781 ttctggtggg acaggtcttg gattatcaat tacaaagcat atagtagagg cccaccaagg
     6841 caatattgaa gtgaatagtc aagttggcaa aggctcaacg ttcaaagtta ttctaaaaga
     6901 ttataaagaa taaaaattaa agtggtaaca gcgcgtgtat ttttcacgaa gttgttgctg
     6961 cttttttatt tcgttcaaat ctctattggt attaaattag agttaatgaa gtggaataaa
     7021 ttcggttgtc aattttgtca tttgtatatg tcgatttgta agttataagt aatagattgc
     7081 tgtgaatcaa gcaattttat aatatattta ttataataaa gaaattttag taacgcgctt
     7141 gaaatagatg cttaattaaa aagtaataag ttttatttga acggaaataa atcttaattt
     7201 aaacacttga atagacaagt gtaatcattc gtcatagaaa aagacgaggg gcgatttaga
     7261 taaattgttg aaagtaatat taaatacctt gtttatctaa tttgattgag gaaacatgca
     7321 taggtgaaaa ttagaaatac attaaaaata ttgaattaat ttttcgacct acaagttgag
     7381 gaaaatttaa ctagatttga ggattagcaa agtgaaaaca attcaattac acaagacaac
     7441 tttgatatta attgcgatat tattgtttta tacttttatg ggtatcttac taccactcat
     7501 gcacgatgat ttacaatggt ttagcaatta taatactgat attttaaaag taggatttgc
     7561 atcacttaat ggtcgctata tcggaaatat ctttgaaatt atagctgtac atgtaagctg
     7621 gttacgttgg ctttcatatg gcctcattag tatgggcatc atttggatga ttatgcacat
     7681 tacacgttgt aaagcgtgga caagctatta tttgttagca ttttctttaa tgttaatttt
     7741 accaagtgct atttatgcag atacatatgg ctggtttgca ggattttata attatgcgac
     7801 atcaacacta atttcactct ttattattta ttattgtatt aatgcaatta tatataaaga
     7861 aaagcagccg gtaagtgtta ctgtactatt ctatgtttta tgcttcttcg gtcaattatt
     7921 tatggagaat gtgacattat tccactgtat cattttaatt ttagcttttc tgtatgaatt
     7981 tactgcaaat cgaacactta attataaatt attgttttca tttatgattg ctactatagg
     8041 tacaatcatt atgttttcca atccaaatta tcgcaaaatt ttatttgaag gatcagaata
     8101 tcaacaagtt tcaaataatc aaggtatctt ttcaaaattt gcagaaatga tatcaacgtc
     8161 acttccatat ggtgtcattt tcagtcagat tataatttta agtatgattg cagcgcttat
     8221 tatttatcta ctgcttagaa gtgatcggta tgtacattta acaatattaa agcgtcgtat
     8281 cattatgata ggatttatca ctttaccgct ttattattta ctattttaca atcagttttt
     8341 attaaataaa aatacagata ttggattggt tggttttgtg aacgtaattg tatgtggtta
     8401 ctttgcgttt tcgatatttg taggtattta tttgtctatt aatgatcgta aaacacaaat
     8461 cactttatat tccttactta ttgcaatatg tgtttcggcg tcgacactag tcattgttac
     8521 tccaatagaa ccaggtaatt ttttgattgt ttatacgata catgtaat
//
LOCUS       DAKTGS010000122         8517 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.122, whole genome shotgun sequence.
ACCESSION   DAKTGS010000122 DAKTGS010000000
VERSION     DAKTGS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.122"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            165..1688
                     /locus_tag="O0286_002253"
     CDS             165..1688
                     /locus_tag="O0286_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170611.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1756..2922
                     /locus_tag="O0286_002254"
     CDS             1756..2922
                     /locus_tag="O0286_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170612.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2919..3839
                     /locus_tag="O0286_002255"
     CDS             2919..3839
                     /locus_tag="O0286_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170613.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3853..4512
                     /locus_tag="O0286_002256"
     CDS             3853..4512
                     /locus_tag="O0286_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170614.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4564..5514)
                     /gene="lytM"
                     /locus_tag="O0286_002257"
     CDS             complement(4564..5514)
                     /gene="lytM"
                     /locus_tag="O0286_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCY0170615.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5840..6832)
                     /locus_tag="O0286_002258"
     CDS             complement(5840..6832)
                     /locus_tag="O0286_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0170616.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            7066..8442
                     /locus_tag="O0286_002259"
     CDS             7066..8442
                     /locus_tag="O0286_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170617.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
BASE COUNT         2869 a         1441 c         1362 g         2845 t
ORIGIN      
        1 attatgtaat caaactattt tttgtagttt attagatttc caataactta ttaaacatta
       61 ttataactaa aattctatta atttaaacaa aatttaaaat aaaccattta ataaaatgta
      121 ttgttctgat atgattaact cataaataat tgggaggaag caaaatgtct aaaaagttaa
      181 aaattataat tcctattatt attgtcttac tattaatagg tggaatcgca tggggagttt
      241 atgcattttt tgctaataca ccgaaaaata catacctaaa aagtgaacaa caaacagcaa
      301 aaatgtataa agattatttt aatgaccgtt ttgaaaatga agtgaagttc caagaaaaga
      361 tgaaagataa ttcgttttta tcttcattag aattaagcgc agatgcatca gatgaaattg
      421 ttaaagggct tggcattcct aaatctgttg ttaatgcatc gaaaattaaa atgtcatatg
      481 gacatgatcc taaaaaagag aaatcaatga ttaatcttga accaacaatc gcagactctg
      541 cattagggaa attccagtta gctgcagata aagataagca ttatttcgaa tcaccattat
      601 ttaaagggaa atatagtgtt aataattctg atttattatc aacttattca aaacttacag
      661 gtgaagatga agaaacagca aaagaaaatg gtattacaaa ccaacaacta aatttaaata
      721 ctctttttaa taatgctcaa gcacaacaaa gtgactacag caaaattgcc gaaaaatatt
      781 ccgaacttat tgtcgacaaa ttagatgacg ataattttga taaaggtaaa aaagaagaaa
      841 ttaaggttaa tggtgaaaag tacaaagtta gacctgtcac gttaacactt agcagagctg
      901 acactaaaaa aattacatta gctgtattag aagaagctaa aaaggataaa gaccttaaaa
      961 aattaatgga agaacaaggt gctacaaaag actttgaaaa agacatcaaa aaagcaattg
     1021 acgatgtcaa agaaactaaa aaggatgaat ttgctaaaat tcaatctaaa atttataccg
     1081 aaaaacatac gattgtaaaa cgagaaatta ctattacaga caaagaaaat aataaaacta
     1141 aaatcaaagg tactaatact ttagaagacg ataagttaaa actagattac gcacttgatt
     1201 tcgatcaaga taaatacacg tatgctgaag cgaaatatac aattaaaggc gtatcttcta
     1261 aagaaaaaga taataaatat aatgataaat acgaatttgg taaaaagaca gaatatgatg
     1321 aatcaaaaat caaattagat aaccaagaaa aagtagatgg cacaaaacgt caagataaag
     1381 gtaaaatcac tgtcgcatta gataaatata gcgacgaaaa tgaattcact tttgaaaata
     1441 atatagattc tgacgtaaaa aataacactc agaaatctac gttaaatatc ggcatcaaat
     1501 atgctgaaga accaattaat ttcattttaa aatctagcac aaaattgaaa gcagatattg
     1561 attttgatga tagtggtgca aaagatttca atagtctatc ttcaaaagac cgtgaaaaac
     1621 ttgaaaaaga aatcgaaaaa aatggcggca aaatgtttga atcaatttta aaaaaggcat
     1681 ctaaataact aaaatataaa aataagattg gaaggtcatc ttgcctttca atcttatcat
     1741 tggaggagtg gttaaatgaa aacaatacat ttgtttcgca tctaccactc ttttttattg
     1801 aaaaagtggt atttgattat ctatttacta tttatattag cggcactcct tattacatta
     1861 acgacaatcc aacatgtaac agaagatgac aatcatttta atataggtgt cgtagataaa
     1921 gatcaatcaa gtgaaacgaa attaatctta aactctattg gtaaagggag taacctagga
     1981 aaaaacgtga gcattaaagc atatgatgat aagcaagcac atactttgtt aaaaaaacat
     2041 aaacttcaag gctattttgt ttttgataaa ggtatgacca aggcatttta taaacaaggc
     2101 gaactaccaa tttcagtata tacatatgat caacaatcca tgaaaagtgt cgtgctatct
     2161 cagctaacag attctgttta ccaacgtctt atgcgatcaa tgggtggcat cttagctttt
     2221 caagacttag caccgaaagc atcacattct gacagtatca atgttatgac tgatttgctg
     2281 attacaggat taaaccgttc aggtgcattt aacttagaac cgattcattt atacgatacg
     2341 ggcagttatt atgcaattac aggattttta acaacggtat tcatctttgc actatcttta
     2401 tttacagttt tgaaaatgaa tcaagatact gtattgaaag cgcgattgaa aatgtttcat
     2461 ttttctaaag agcgtttatt aatcattcgt acgttgatta catggtttta tactatgtta
     2521 tggagtatcg ttggtgtagt ttggattgtg ttcagtattc cgaatatctt tgaattatat
     2581 aattggccaa cgttagccat tcatttaagc tattatgtta cctttttaat actgtggtta
     2641 ttactcatcg aacttttaac aacaggcttg ttgaatagta ttagtaaagt catattagct
     2701 attgtcatcc tggtgttatc tggtttaaca atacctacga tttttttaca acatatagca
     2761 aatggtgttt tcaatattca accgttcgca gttgtaacaa atcaattgtt agaaattatt
     2821 ttaaacaact acattttaga actgcaccct agtttctatc ttagcttcat cgcacttttg
     2881 ataattaatt tagctgtatt ggtttggagg tatcgtcaat gaagccttat atacaacttg
     2941 ttgtgttcaa gcaatggtta caatacatct tgctcgtaac aaccattgtc atcgcactcg
     3001 tacttattgg tatcggttac cgtgtagcac atgacaactt caaaataccg attaccattc
     3061 aagatttaga ccaaaccact gcatcaaaat cattcgtcaa taaaattaaa caatctgact
     3121 atgtaactat taaaaaagtc gatgaagatg aaagctatat tgaagatgat gttactaaaa
     3181 aggaagctat tttaagtatg caaattccta aaggtttctc tcaaaaatta aaagagaacc
     3241 gtttaaaaga aacgatacag ttatatggta gagatgactt tataggtggt attgctgtag
     3301 aaattgttag tagttcatta tatgagcagc aaattcctaa cattatttat gaacaccttg
     3361 aggatatgaa acagcatcaa tccatcgatg ctatcaacaa gtcctatcat aaacatacac
     3421 ctgaatctaa aatcaaattt gtgtcgctta ctaaacaagc acaacactct atttcaatta
     3481 gcttaatctt tgcggtgatt ttatttgtta gcgctgttca agtagtactt cattatcgtt
     3541 taaaccaaca agcagcattg caacgattat cacaatatca tttaagccgt ttcaaactat
     3601 atagtactta tgtaatgaca catacgattt tgttattgtt ggtactattg gcagttagtc
     3661 tatatttgtc tcaaccactc agcttaatat tttacttaaa atcactgtta cttatattga
     3721 tttatgagat aggtatcgtc tttatcttat tccatattca aacaataagt catcgattat
     3781 tcatgacatt tatatatgca cttgctatgg gtatcgtata cttgattatt ttcatgtaaa
     3841 ggagcgtaac tgatgataga aattaataac ctttcaaagc gttaccgtaa caaacagatt
     3901 ttcaatcatt taactatgtc ctttgatagt aatcgtttaa ccgtattact tggtgataat
     3961 ggtgctggaa aatcaacatt acttcgtatg attgctggta ttgaaaaagc taatgatgga
     4021 actatcaact atttcggcga aaaatggaat caaagacaaa tacaaaatca catcggttat
     4081 gtgccacaag acattgcgtt atttgaacac atgacagtgg ctgaaaacat taaatttttt
     4141 aaatcacttt gtaaaaatcc aattaacgat acaactatca acgaatattt acagcaatta
     4201 aactttgatg atacgtctgc caaagtatct acattgtccg gtgggaataa acgtaaaatt
     4261 aatatattag taggtttact aggtcaacct cgaattctca ttttagatga accgacagtt
     4321 ggtattgatt taaaatctag acatgacatc caccaactac ttaacatcat gaaatctaaa
     4381 tgtttaatta tattaactac ccatcattta gatgaagttg aagcacttgc agatgatatc
     4441 aagttaattg gccaagatcc tttttatcaa catgttttag aggacaaaca atggacttat
     4501 acctattatt aaaacgaaaa aatcccaagc tgcgtatgat atcgcaactt gggattttct
     4561 gtattatcta ctttgcaagt atgacgttgg gtctactgca tattgattac cgatgccacc
     4621 agacatacgt tggaagtgta cgtgaggcgc tgttgaatta cccgtactac ctgaatatgc
     4681 aatttggtca ccagctttga ctttatcacc agctgaaaca gttaaacgat tattatgcat
     4741 ataccattgg tagttattac tgttcgcttc tttaatcgtt acttgattgc cgccaccata
     4801 gttactccaa cctgcttgta ctactgtacc atcagttaat gagtaaactg gtgaattttc
     4861 aggcattgca tagtcgacac cgtaatgcgc accaccaccg tgatattgtc catatggttg
     4921 tagttgttta cgacttgtta accagcttgc gtctttcgca tgaccactag ctgtcgcttt
     4981 acttgctgat ccaccatttt gattagatgt gccattagga taattgacct ttccattacc
     5041 atcatggctg ttatacgctt ggttgttgtt actatgtgaa taatagctcg cgtctggacc
     5101 tacatttgat tgataaccat attgattaat atgctgttgg ctttgactcg ctgtgtagtc
     5161 attgttatct cctgctgttg ctggattcac atatgtttgg ccgcttccat tggcatttgc
     5221 attttttgga taacagttat aaaaataatg cgtatgtcct tgagcatcta cgaatgtata
     5281 gctatattct ttattatcaa acattgcttg attccagtta ccatcaggtg tgtgatgata
     5341 atccccatta gaatcaattg tataataagt accataagat acgtcttgtg attgtgttga
     5401 catttgtgta tgtgcttgtt gggtgtttgt cgtttctgct gcatctgctt gatgcgccat
     5461 tgtaaatgta gcgaagccca tcgttgcaat cgctgctgct gttaattttt tcatgtataa
     5521 aacatcctcc attaaagtta aagttagttt tcaattaaac tgtactgcac atactaaaag
     5581 aattagacaa ctgagtaaag gatttaattc tcattttcca actatttaat attcccgaaa
     5641 tgttttacta aactcattac attgtcatta caaaatagcc atacattgat attaaaatga
     5701 catctctcac tgcattcgtt taacctttta taaattttca aaattaacaa ctaatcgttc
     5761 gtccatgttc gcgattcaac gctaatgcat gatagtaatc atccatcaaa tcatatcaac
     5821 caaattccat tatcaatcgc tattgattgt cattcaactt tctaatagtg atatgcttct
     5881 caggcttaaa aatcgtcata tcttttctat taattaaatc atctgtgagc tttaatgcta
     5941 ctaattcatt gctgccataa tacttaatat ataacgttct tgtagttaaa tttattacgg
     6001 tctgatacat cgtatagtga ttgtcagcat catgcggacg tacaattcca atcggtatat
     6061 ttaccgcatc taataaataa aatgcattca ttaaatccat ttctttatca ttgttttgag
     6121 caatgtttgc tttcataaat gccattctca caaagcgctc agttgaagta aatccacctg
     6181 gcaatccaaa tgtacctgct tcattgccta aaggttcaat cgttacacct tccaataaat
     6241 ttgctgttgc tggataagga gaaatattga tatattgtct taaattacta taatgccaat
     6301 ttaagtctgg atgatttgtt aagacaccaa taggattatc ttttataacc acttcaccct
     6361 ctttaaatga aacttcgact gtatgtccag ttgcatcgga aacatgataa tgcaatggcg
     6421 gaacttcacc gatgtcattt aaatatacag ctacaacatg tatttgggat gcttgttgtt
     6481 tcatatcttc aatgcttgtt gtatatccca aaatccatgt cacaatttca ttttgcgtaa
     6541 tattcatcgc gtccgctttg tgtgttgatc catatgaact ataacctcgg aaatattgtg
     6601 ttgaaatggc aacgccatgt tcattaacac catcaccata aataaaacct tccatatctg
     6661 ttcctgtgcc aataaagcca tattgcgttt ggcctgtcgt gccagtgcga gatttccaac
     6721 gataatttct aggcgtcact gctggcgaac catctaatgg ataatcataa tccatcgtgc
     6781 gtccaagaag tacttgatta tttaaagttt gtattgtgaa tcctgtgcac attgttctca
     6841 ctcctctgta ccttcattta ctttaatcac tttcaaataa agctgtttca cttaaacata
     6901 ctataaaaaa tcaattatac aagcaattaa ttgatattca ttctcaataa ctgtggtatg
     6961 atatgtaagg aaatcatgac ttatgtgtga gtgaacgatc atctatacat ccgttcactt
     7021 catctcatga ctttctatat ttaattttta caaggagtga catctgtgaa taacacacaa
     7081 tcttcaccac gcagtaatat tattattgcg attatgttgt ctgcattaac atattggttg
     7141 tttgcacaat catttattaa tataggacct ctcgttggtc aaacgtatca aacctctcct
     7201 gccgtgttaa atttatctat tagtttaact tccttcgcca caggtatctt catggtggct
     7261 gcaggtgata ttgctgataa aataggacaa ctgagaatga catacatggg tctcataatc
     7321 agtatgtttg catctcttct attaattata tcggacatca ctgcactgct catcatcggt
     7381 agaattttac aaggtctatc agcagctatc ttgttacctt caacagttgg cgtgttaaat
     7441 aatcaattta aaggagaaca tttaagacga gcgattagtt atctaatgat tagtactgtt
     7501 ggtggcatcg gcctagctgg tgttatcggc ggtttaattg cctcaaattt cggatggcaa
     7561 acgaatttca tcattagtat agtcattgct ttcattgcca tattgcttct aaaaggcaca
     7621 cctgaaaaag taagtcaaca tagccaccgt catccattcg attacaaagg tatgtcgatt
     7681 ttcgctgtta tgattggtag ctttacatta ttgttaacac aaggattcga acaaggttgg
     7741 tttagtacat tttcaatcat ttgtctgagc atttttatca tcactacgtt gatattcatc
     7801 atcatcgaac gtcgacatga agtacctttt attgatttct cagtattacg caaccgtccg
     7861 ttcattggtg catttttaaa taactttgtt ttaaatagcg gtctaggcgt aacagtggtc
     7921 tttttcatat atgctcaaac acaccttggt ttatcagctg cgcaatctgg acttgttaca
     7981 ttgccatatg ccattgtggc agttgcgatg attcgtttag gtgaaaaagc aacattacgt
     8041 ttcggtggca aattgatgct catcattggt ccgttgttcc ctgtcatcgg catcactatt
     8101 attagcatga ctcaattatt ggcatcacaa tatgtcattg cagttatcat tggtttcgtc
     8161 atatgtgcga taggtaatgg tttagtcgca acacctggac ttacgattgc aattttcagt
     8221 atgcctaatg aaaaagttgg tttagctaca ggattatata aaatgagtgg tacattaggt
     8281 ggcgcctttg gtatagcact aagtactaca gttttcagta tgttacaact aaactatgca
     8341 ccaagtgtag ctgcaaccgt aacatttata gtcagcattg tattgatgat ccttggctca
     8401 ttgtctgcat acatgatcat tccaaaaaca gttaaatctt aaatataata gaagaattat
     8461 gtttcgaaat atctttatca ctttaaaatg atatacaaga aatccaagaa aaataag
//
LOCUS       DAKTGS010000123         8473 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.123, whole genome shotgun sequence.
ACCESSION   DAKTGS010000123 DAKTGS010000000
VERSION     DAKTGS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.123"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            3..653
                     /locus_tag="O0286_002260"
     CDS             3..653
                     /locus_tag="O0286_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0170618.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            650..886
                     /locus_tag="O0286_002261"
     CDS             650..886
                     /locus_tag="O0286_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCY0170619.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            1155..3140
                     /gene="uvrB"
                     /locus_tag="O0286_002262"
     CDS             1155..3140
                     /gene="uvrB"
                     /locus_tag="O0286_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCY0170620.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            3148..5994
                     /gene="uvrA"
                     /locus_tag="O0286_002263"
     CDS             3148..5994
                     /gene="uvrA"
                     /locus_tag="O0286_002263"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCY0170621.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            6656..7588
                     /locus_tag="O0286_002264"
     CDS             6656..7588
                     /locus_tag="O0286_002264"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCY0170622.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            7594..8433
                     /locus_tag="O0286_002265"
     CDS             7594..8433
                     /locus_tag="O0286_002265"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCY0170623.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
BASE COUNT         3054 a         1156 c         1706 g         2557 t
ORIGIN      
        1 aaatgggtgt acatcaatat tttaaaagat tatcagatat ggaaagactt ataagattac
       61 ctggaaaatt taaatatttt gaacacaatg ttgcagcaca ctcctttaaa gtaactaaaa
      121 ttgcacaata tctagcaaca gttgaagaat atcatggacg aaagattaat tggaaaagct
      181 tatatgaaaa agcattaaat catgatttcg ccgaagtgtt tactggtgat ataaaaacac
      241 ctgttaaata tgcgagtagt gaattaaaaa aattattttc gcaagttgaa gaagaaatgg
      301 tagagacctt tattgaagaa gaaattccat tacaatatag agatgtttat aagcaacgac
      361 tgcaagaagg taaagatgat tcattagaag gccaaatact ttcagttgct gataaaattg
      421 atttgcttta tgaaacattt ggagaaatac aaaaacgtaa tcccgaagaa ttatttttcg
      481 aaatttatga aatgagtcta gaaacaatta ttcaatttga ccatttagca tctgtacaag
      541 attttattaa taatatcatt ccagaaatgt tgactgaaaa ctttatacct agaacagaat
      601 taagagaaac aaccatgaac attttaaata aaagaaaaga ggaaaatgaa tgatatggta
      661 ttttagcgca gcattctttc catgtgtctt ggtagtatta tttagtgtaa taacaagaag
      721 taaatgggtc ggtactattc tgacattaat tttaattggt gcctcaatct ataaagagta
      781 tttccataac gagtggatta tttttattga tgtagtgtca ttattagctg gttatttaat
      841 tatagatcaa ctcgaatttc ataaacatca agatgaagat cgctaagatt aactttaaaa
      901 taatgtttca aacaaatttg ttgaaacaaa atgatgatta atataatgtg tatttacata
      961 ctaaaaataa caagataaac gatttgattt aaggcaagca tagttagcac aactatgttt
     1021 gtttttcttt gttcgacatt tttacgaaca aacgtttgct ttttgtgtga ctactttgct
     1081 aaaatatgta atgagaaagc aaagagttga gcggaaatat aaataatacg ttgaaagagg
     1141 aggcatatgt gacaatggtt gaacattatc cttttaaaat acattctgat tttgagcctc
     1201 aaggtgacca accgcaagca attaaagaaa tcgtggaagg tattaaagcg gggaaaagac
     1261 atcaaacttt attaggtgct actggcacag ggaaaacatt tacgatgagt aatgttatta
     1321 aagaagttgg gaaaccaacg ttaattatcg cgcataacaa aacattagca ggacaattat
     1381 atagtgagtt taaagaattt tttcctgaaa acagggtgga atactttgta agttactatg
     1441 attattatca accagaggca tacgtaccgt ctactgacac ttttattgaa aaagatgcct
     1501 caatcaatga tgaaattgat caactacgac attctgctac aagtgcatta tttgaacgcg
     1561 atgatgtaat tattattgct agtgtaagtt gtatatatgg tttaggtaat cctgaagaat
     1621 ataaagattt agtagtaagt gttcgagttg gtatggaaat ggatagaagt gaattactta
     1681 gaaaacttgt agatgtgcaa tatacacgaa atgacatcga tttccaacga ggaacgtttc
     1741 gagtgcgtgg tgatgtagtg gaaatattcc cagcctctaa agaagaactt tgtataaggg
     1801 ttgagttttt cggcgatgag attgaccgta tccgagaagt taactaccta acaggtgaag
     1861 tgttgaaaga aagagaacat tttgcgatat tcccagcttc tcacttcgta acacgtgaag
     1921 aaaagttgaa agttgcgatt gaacgtattg aaaaagaatt ggaagaacga ttgaaagaat
     1981 tacgagatga gaataaatta ctagaagcgc aaaggttaga acagcgtacc aactatgatt
     2041 tagaaatgat gcgagagatg ggattctgtt caggaattga aaactattcc gtacatttaa
     2101 ctttgcgacc actgggttcg acaccatata ctttattgga ttactttggc gatgattggt
     2161 tagtaatgat tgatgaatca catgtgacat taccgcaagt tcgaggcatg tataacggag
     2221 acagagcgcg taaacaagtt ttggtggatc atgggtttag attaccgagt gcattagata
     2281 accgtccact taaatttgaa gaatttgaag aaaagacaaa acaacttgtg tatgtatctg
     2341 caacgcctgg accatacgaa attgaacata cggataagat ggttgaacaa attattcgtc
     2401 ctactggttt actggatcct aagattgagg ttagacctac tgaaaatcaa attgacgatt
     2461 tattaagtga aattcaaaca agagttgagc gtaatgaacg cgtacttgtt acaacgctca
     2521 ctaaaaagat gagtgaagat ttaaccacat acatgaaaga agcgggtatt aaagttaatt
     2581 atctgcattc agaaatcaag acattagaac gaattgaaat aattagagac ttacgaatgg
     2641 gtacatatga tgttatcgta ggtattaatt tattaagaga gggtattgat ataccagaag
     2701 tttctctagt tgtcatatta gatgcagata aagaagggtt tttacgttct aaccgctcat
     2761 taattcaaac aataggtaga gctgcgcgta acgataaagg tgaagtcatt atgtatgccg
     2821 ataaaatgac tgattcgatg aagtatgcaa ttgatgagac acaacgtcgt cgagaaatac
     2881 agatgaaaca taatgaaaaa catggtatta cacctaaaac aattaataaa aaaatacatg
     2941 atttaattag tgctactgtt gaaaatgacg aaaataatga caaagcacaa actgtgatac
     3001 ctaagaagat gacgaaaaaa gaacgtcaaa agacaatcga caatatagaa aaagaaatga
     3061 aacaagcagc gaaagattta gatttcgaga aagctacaga attaagagat atgttatttg
     3121 aattaaaagc agaagggtga caagtaaatg aaagaaccat ccatagtagt aaaaggtgct
     3181 cgtgcgcata acttgaaaga tattgatatc gaactaccta aaaataaatt aattgttatg
     3241 acaggtttat ctgggtcagg taaatcgtca ttagcattcg atactatata tgctgaagga
     3301 caacgacgtt atgttgaatc attaagtgcc tatgcgcgtc aatttttagg ccaaatggac
     3361 aaaccagatg ttgatacaat tgaaggatta tcgccagcaa tttcaataga tcaaaaaaca
     3421 acaagtaaaa atccaagatc aactgtagca acagtaacag aaatatatga ttatatacgt
     3481 ttgttatatg cacgtgttgg taaaccttac tgtccaaatc acaatataga aattgaatcg
     3541 caaacagtac aacaaatggt tgaccgcatt atggaattag aggcacgtac aaagattcaa
     3601 ttattagcac ctgtcatcgc tcatcgtaaa ggtagtcatg aaaagctaat cgaagatatt
     3661 ggtaaaaaag gttatgtacg tttaagaatc gatggcgaaa ttgttgatgt aaatgatgta
     3721 cctactttag ataagaacaa gaatcataca atagaagttg ttgtagaccg attagttgtt
     3781 aaagatggaa ttgaaacacg actagctgac tctatagaaa ctgccttaga gctttcagaa
     3841 ggacaattaa cagtcgatgt cattgacggg gaagacctta agttttcaga aagccatgct
     3901 tgtcctatat gtggattttc aatcggagag ttagaaccaa gaatgtttag ctttaacagt
     3961 ccttttggtg cttgtccgac atgtgatggc ttaggccaaa agttaacagt cgatgtagac
     4021 ttggttgttc ccgacaaaga taagacgcta aacgaaggtg caatagaacc ttggataccg
     4081 acgagttctg atttttatcc aacattgtta aaacgtgttt gtgaagttta taaaatcaat
     4141 atggataaac cttttaaaaa gttaacagaa cgtcaacgtg atattttatt gtatggttct
     4201 ggtgacaaag aaattgaatt tacatttaca caacgtcaag gtggtactag aaaacgaaca
     4261 atggttttcg agggtgtagt tcctaatata agtagacgat tccatgaatc tccttcagaa
     4321 tatacacgtg aaatgatgag taaatatatg actgaactac cttgcgaaac ttgtcatgga
     4381 aagcgattga gtcgtgaagc gttatctgtt tatgtaggtg gtttaaatat tggtgaagta
     4441 gtcgaatatt caatcagtca agcgctgaac tattataaaa acattgattt gtcagaacat
     4501 gatcaagcga ttgcaaatca aatattgaaa gaaattattt cccgactcac ttttttaaat
     4561 aatgtgggac ttgaatattt aacgttaaac agagcttcag gtacactttc aggtggtgaa
     4621 gcacaacgta ttcgattagc aacgcaaatt gggtcgcgtt tgactggtgt cttatatgta
     4681 ttagatgagc catcaattgg actgcatcaa agagataatg atcgattaat taatacactt
     4741 aaagaaatga gagatttagg aaatacttta attgtagttg aacacgatga tgatacaatg
     4801 cgtgcggctg attacttagt ggatataggt cctggtgctg gtgaacatgg agggcagatt
     4861 gtgtctagtg gtactcctca aaaggtaatg aaagataaaa aatcattaac aggacaatac
     4921 ttgagtggta agaaacgtat tgaagtacct gaatatcgca gaccggcttc agatcgtaaa
     4981 atttctatac gtggagctag aagcaacaat cttaaagggg ttgatgtgga cataccacta
     5041 tcaatcatga cggttgttac aggtgtatca ggttctggta aaagctcatt agtaaatgaa
     5101 gtattataca aatcattagc tcaaaaaatt aataaatcta aagtaaagcc aggattgtac
     5161 gataagattg aaggtattga tcaacttgat aaaattattg atattgatca atcaccaata
     5221 ggtagaacgc cacgctctaa tccagcaaca tatactggtg tgtttgatga tatacgtgat
     5281 gtgtttgcgc aaacaaatga agctaaaatt cgaggatatc aaaaagggcg ttttagtttt
     5341 aatgtaaaag gtggacgctg tgaagcttgt aaaggtgacg gtattattaa aattgaaatg
     5401 cattttttac ctgatgttta tgttccttgt gaagtgtgtg atggtaaacg atataatcgt
     5461 gagacactag aggttactta caaaggtaaa aatattgctg acattttaga aatgactgtt
     5521 gaagaagcaa cacaattttt tgaaaatatt cctaagatta agcgcaagtt acaaacacta
     5581 gttgatgttg gtcttggata cgtcacatta ggtcaacaag ctacaacgtt atcaggtggt
     5641 gaggctcaac gtgtgaaact tgcatctgaa cttcataaac gttcaactgg taaatctatt
     5701 tatatcctag atgaaccgac aacagggtta catgttgacg atattagtag attattaaaa
     5761 gtattaaacc gattagttga aaatggtgat actgttgtaa ttattgaaca taacctagat
     5821 gttatcaaaa cagcagacta tattatagac ttaggtcctg aaggtggtag tggcggtggt
     5881 actattgttg cgactggcac acccgaagat attgctcaga caaagtcatc atatacagga
     5941 aagtatttaa aagaagtact tgaacgagat aaacaaaata ctgaagataa ataagattaa
     6001 aagaagtgaa ggatgttata aatttatcct tcgcttcttt ttattaattt agtaatgaat
     6061 agtagaaaga aaagatgcgt aaaaagaatt atgttaagat agggtcaatc tagagtagtt
     6121 aaacataaat cgaactggga gtgggacaga aatgataaag aatcactaat gatttattat
     6181 gtagtggttc tttgtcatta gccacagcta ttgtgtactt aaaaatagga atgcatgagt
     6241 gcaactcatg cataagaaat actaatttct aaagaaaaag tatttcttta tgttggggcc
     6301 ccgccaactt gcattgtttg tagaatttct tttcgaaatt ctttatgttg gggccccgcc
     6361 aacttgcatt gtttgtagaa tttcttttcg aaattcttta tgttggggcc ccgccaacta
     6421 attccaatat atcattgtag agcttaggtc attgattttt ggctcggact tttatggcga
     6481 tatgaaccat gtaaattaag caagcaataa attaatgatt gatattgact tgtaaaataa
     6541 taacaataat gaacaattaa tatttatttt agcttttcaa tgtagattgg tgttatattt
     6601 ttgatatgat aagaagagat gtaagagtag ggataaatac aattgaggtg aacccatgtt
     6661 aacgacagaa aaactagttg aaacattaaa gttagattta atcgctggtg aagaaggact
     6721 atcgaagcca attaaaaatg ctgatatatc aagaccgggc ttagagatgg caggttattt
     6781 ttcacattat gcgtcagata gaatacaact attaggaaca acggaactat cgttttacaa
     6841 tttattacca gataaggatc gcgcaggtcg tatgcgtaaa ctatgcagac cagaaacgcc
     6901 tgcaattatt gtgacacgtg gattgcagcc accagaagaa ttagttgaag ctgcaaaaga
     6961 attaaatacc ccacttatag ttgctaaaga tgcgactaca agtttaatga gtcgcttaac
     7021 aacgttttta gagcatgcac ttgcaaagac gacatcttta catggtgttt tagtagatgt
     7081 ttacggtgtt ggtgtactaa ttaccggtga ttcaggaata ggtaaaagtg agactgcgtt
     7141 ggaattagtt aaacgtgggc atagattagt agcagatgat aatgtagaaa tacgtcaaat
     7201 taataaagat gaactaatag ggaaaccacc aaagttaata gaacatctat tagaaatacg
     7261 tggactaggt attatcaatg ttatgacttt atttggcgcg ggttcaatat taactgaaaa
     7321 acgaattaga ttaaatatta atttggaaaa ctggaacaag caaaagttat atgaccgcgt
     7381 aggtcttaat gaagagacgc taagtatttt agatactgaa atcactaaaa aaacaatacc
     7441 tgtaagacct ggtagaaatg ttgcggtaat tattgaggtc gctgcaatga actatcgatt
     7501 aaatatcatg ggcattaaca cggccgaaga atttagtgaa agattaaatg aagaaattat
     7561 caagaacagt cataagagtg aggagtaggt tgaatgggta ttgtatttaa ctatatagat
     7621 cctgtggcat ttaacttagg accactgagt gtacgatggt atggaattat cattgctgtc
     7681 ggaatattac ttggttactt tgttgcacaa cgtgcactag ttaaagcagg attacataaa
     7741 gatactttag tagatattat tttttatagt gcactatttg gatttatcgc ggcacgaatc
     7801 tattttgtga ttttccaatg gccatattac gcggaaaatc caagtgaaat tattaaaata
     7861 tggcatggtg gaatagcaat acatggtggt ttaataggtg gctttattgc tggtgttatt
     7921 gtatgtaaag tgaaaaattt aaacccattt caaattggtg atatcgttgc gccaagtata
     7981 attttagcgc aaggaattgg acgctggggt aactttatga atcacgaggc acatggtgga
     8041 tcggtgtcac gcgctttttt agaacaatta catttgccta attttataat agaaaatatg
     8101 tatattaacg gccaatatta tcatccaaca ttcttatatg aatccatttg ggatgtcgct
     8161 ggatttatta tcttagttaa tattcgtaaa catttaaaat taggagaaac attcttttta
     8221 tatttaactt ggtattcaat tggtcgattc tttatagaag gattacgtac agatagctta
     8281 atgctcacaa gtaatattag agttgcacaa ttagtatcaa ttcttttaat tttaataagt
     8341 ataagtttaa ttgtatatag aaggattaag tataatccac cgttgtatag caaagttggg
     8401 gcgcttccat ggccaacaaa aaaagtgaag tagtgatagt ttgaggaaat ttttatcaaa
     8461 aacacatcat cat
//
LOCUS       DAKTGS010000124         8382 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.124, whole genome shotgun sequence.
ACCESSION   DAKTGS010000124 DAKTGS010000000
VERSION     DAKTGS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.124"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..282)
                     /locus_tag="O0286_002266"
     CDS             complement(<1..282)
                     /locus_tag="O0286_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCY0170624.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAID"
     gene            544..729
                     /locus_tag="O0286_002267"
     CDS             544..729
                     /locus_tag="O0286_002267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170625.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(952..1530)
                     /locus_tag="O0286_002268"
     CDS             complement(952..1530)
                     /locus_tag="O0286_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0170626.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1794..2174)
                     /gene="blaI"
                     /locus_tag="O0286_002269"
     CDS             complement(1794..2174)
                     /gene="blaI"
                     /locus_tag="O0286_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0170627.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2164..3921)
                     /gene="blaR1"
                     /locus_tag="O0286_002270"
     CDS             complement(2164..3921)
                     /gene="blaR1"
                     /locus_tag="O0286_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCY0170628.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4028..4873
                     /gene="blaZ"
                     /locus_tag="O0286_002271"
     CDS             4028..4873
                     /gene="blaZ"
                     /locus_tag="O0286_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0170629.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5399..5818)
                     /locus_tag="O0286_002272"
     CDS             complement(5399..5818)
                     /locus_tag="O0286_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0170630.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5942..6913
                     /locus_tag="O0286_002273"
     CDS             5942..6913
                     /locus_tag="O0286_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0170631.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(7085..7177)
                     /locus_tag="O0286_002274"
     CDS             complement(7085..7177)
                     /locus_tag="O0286_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0170632.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7334..7681)
                     /locus_tag="O0286_002275"
     CDS             complement(7334..7681)
                     /locus_tag="O0286_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0170633.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7700..8317)
                     /gene="cadD"
                     /locus_tag="O0286_002276"
     CDS             complement(7700..8317)
                     /gene="cadD"
                     /locus_tag="O0286_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCY0170634.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         2972 a         1225 c         1099 g         3086 t
ORIGIN      
        1 atcaatggca cgatataaat agctccattt tccttttatt ttgatgtacg tctcatcaat
       61 acgccatttg taataagctt ttttatgctt tttcttccaa atttgatata aaattggggc
      121 atattcttga acccaacggt agaccgttga atgatgaacg tttacaccac gttcccttaa
      181 tatttcagat atatcacgat aactcaatgc atatcttaga tagtagccaa cagctacagt
      241 gataacatcc ttgttaaatt gtttatatct gaaatagttc atacagaaga ctcctttttg
      301 ttaaaattat actataaatt caactttgca acagaacctc atttactata tgggcatctc
      361 tttcattatt aatacaaagc tttgtatctc cgatattagg caaaggtgta aacgaattag
      421 gtgatactat aggattcaac attatattaa tcgtttcttt aataatgatt atcttcttat
      481 tttttataaa tcgtagtaaa aatatctcct atgattatat aaaagttcaa agaggtgaag
      541 ccaatgaata aattagaaaa atggtctttg gtatttatta tttttacatt tctctcttca
      601 ttagcacttt tatatttcaa atataatttt agttcggtta tgccagtcct aagtctttct
      661 attttaacta tactaatatt gttgacgagt attattactg tagtttcagt taatgatgaa
      721 gatacttaaa acgttcatta gtgaaacagg tattatatta aaaaagcgta cgaatttgta
      781 cgcttttttc ttgtgttatt attaaaatct cataacagga aaatattatg agattttaga
      841 cgctgtcaga acgtctcttt tacatcgagt gacattgaat caaagtcttt gtatgttaat
      901 gatatataaa attatttagt agattcatta tagtaagcta gattagttgc tctacttgtt
      961 tttattctct tttgcctcta atttattgag ataacgataa atagttgtac gtgaaacttg
     1021 ccactgctct gcaattgttt taattggcgt accattatca taaagtgtct taagtaagtt
     1081 taaatcttgt ttatttaatt tttcaggtct acctccatat cggcctctag cacgtgctgc
     1141 aattcgaccg gctgatgatc gctctaaaat taaattacgt tcaaactctg caaatgctgc
     1201 gaataaatga aataataatt gtcctgtaga acttgattta tccatcgtta tattttcttc
     1261 taaactatga aaactcacac ctcgttcgtt aagttcatta actaacgtga ttaaatcctc
     1321 catgttccgt cctagtctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa
     1381 ttcaattgct ttatctaaac caggtcgctt actttttgaa ccactcatat ggtcattaaa
     1441 tatcttttca caaccatatg tatttaatcg atcctcctgt aaattcaaat tttgtaatcc
     1501 agttgaaact cttgcataac ctattttcaa aatacagacc tcctatatac acctttagtg
     1561 taccataact tgattacgag gtcttttgtg aacatagatt ttttgttggg tttttgaaca
     1621 cataattcta tgtaatactt acttatttag acaaaaaaca agagtgtaca gaaaatggtt
     1681 attttctgga cactctcatc tttaataata tattttaaat gtcattaaca aaactaattt
     1741 aataagagtc aagtatggtt tacatatgta ttatgtttga aacatacctc taattacttt
     1801 ttactaatat catttaaaat gtctcgcaat tcttcaattt ctttgttatt taattcttca
     1861 tttttcgcaa aattcagcac taaacttttc atatcccctc catacagttt attaagaaag
     1921 gttttagcag ttttcatttt aatatcgtct tctttaatat ttgatgagta aaaataaata
     1981 ttttctgatt tgtatcgttt tataatctct tttttatata gtcttgtgat taatgttcta
     2041 atcgttttat cactaacttc tttatatttt tgaatttcaa ctacaatttc attagctgat
     2101 actgattttt tatcccatat tatattcata acatcccatt cagccataga tatttcaact
     2161 tgcttattgg tcatttaaaa cacccattcc ttttaatatt ttttcactta ttaattcagc
     2221 atttttccca gacgggtttc catctgataa atgtgtagca aaataatact tatcatgatt
     2281 tgtaattacg taacctacaa accacccatt attatacttc ccgtttacta tacctgtacc
     2341 tgttttccca tacagttcat acttttcatt tttcttaatc aataatgaag aagataattg
     2401 attctttgct tttttactaa aatggttatt ttgttccatc atatttttaa aaactattac
     2461 ttgttcaaga ttagatattt tcaaactatc ttccatccaa tagcttttat aacttcccaa
     2521 attttcatta ccataattta attgcttgag ttgagccgca gtatagttct ttggtatttg
     2581 attactaata cgttcgaagt accaattaac tgaattttgc attgctgtat ttaaatcttg
     2641 ttccttattc caagactcga aaagataatg tttatgattc catgacatac gtgaattttt
     2701 atcacttata atatgacggt caagtccaaa cagtgctaaa taaattttgt aagttgaatc
     2761 aggagaatat ctttttctac tttctttttc attataaata tagtatttgt ctttcttcat
     2821 gctgtacata acgaaagatc cactatttga accaaaattt ttactttcat ctaaaatttg
     2881 gtaatcgctt tgtaagggct ttttataatt ataatcagtt aaggattgcc ccattaaaaa
     2941 ctggctttga attatcatga taaaaaaagt aaaaatacaa ataaaaatta gaattaattt
     3001 tgactgcttt ttcagatcgg cttcttttat attaattaat ctacttttga gtaatgattt
     3061 tttaccatta aatgaatggc ttaatatatt tttgttggaa aatggggttt ttaatacgga
     3121 gtccataata gattccgcat aagttttaaa ttcatttttg ttaatattat taaggacaaa
     3181 tctatcggct tctacttcat tgtcatgaat tatctttctt tttacaatat gtactaatgg
     3241 attgtaactc ataataatag agaagatatt aaagataatt aaatgtaaag tatctctatt
     3301 tttagcatga gcatattcat gcagtattat atattttagt cttttatcaa ttacactttt
     3361 aaaatatgaa ctaggaatca aaataatata tttcccatac caaaaagtta ttggagattg
     3421 aatagcctct gcttttcgaa tcacaatatt ttttttatat tgatggttga aaagtatcgt
     3481 atctatttta tttttttcat tttcgtttag ataaagtgac tgtttcttta aatatttaag
     3541 atataataag gctttcaaaa atttaaaact taaaataata actaaaacta tccaaataac
     3601 tgtgcagata ttatcaattg aatcccaatt aaacttatgg atatctgttg tgaactcttg
     3661 aataggtttg gtggtattta tgttatggtt caaatcgtgt gacttacttt caactgtggg
     3721 cgcttgatta ttcacattat taaattttat aaaagagaat ttaattggga taaaaggaat
     3781 taatccagca agcatagtta gataccaaac tttataattt aaactataat taaaatagcg
     3841 ttttaatatg tacctaaaaa atactaacaa tagaaatata aaacaaaagc ttactacgct
     3901 cattattagt aatttagcca ttttgacacc ttctttcaaa tatttataat aaacaattga
     3961 catcaatatt acaattgtaa tattattgat ttataaaaat tacaactgta atatcggagg
     4021 gtttattttg aaaaagttaa tatttttaat tgtaattgct ttagttttaa gtgcatgtaa
     4081 ttcaaacagt tcacatgcca aagagttaaa tgatttagaa aaaaaatata atgctcatat
     4141 tggtgtttat gctttaaata ctaaaagtgg taaggaagta aaatttaatt cagataagag
     4201 atttgcctat gcttcgactt caaaagcgat aaatagtgct attttgttag aacaagtacc
     4261 ttataataag ttaaataaaa aagtacatat taacaaagat gatatagttg cttattctcc
     4321 tattttagaa aaatatgtag gaaaagatat cactttaaaa gaacttattg aggcttcaat
     4381 gacatatagt gataatacag caaacaataa aattataaaa gaaatcggtg gaatcaaaaa
     4441 agttaaacaa cgtctaaaag aactaggaga taaagtaaca aatccagtta gatatgagat
     4501 agaattaaat tactattcac caaagagcaa aaaagatact tcaacgcctg ctgctttcgg
     4561 caagacttta aataaactta tcgcaaatgg aaaattaagc aaaaaaaata aaaatttctt
     4621 acttgattta atgtttaata ataaaaacgg agacacttta attaaagatg gtgttccaaa
     4681 agactataag gttgctgata aaagtggtca agcaataaca tatgcttcta gaaatgatgt
     4741 tgcttttgtt tatcctaagg gccaatctga acctattgtt ttagtcattt ttacgaataa
     4801 agacaataaa agtgataagc caaatgataa gttgataagt gaaaccgcca agagtgtaat
     4861 gaaggaattt taatattcta aatgcataat aaatactgat aacatcttat agtttgtatt
     4921 atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt ttccctttat
     4981 tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa
     5041 aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg
     5101 tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat
     5161 caagcaaagt gacaatagca ttttacaagt attttttatt gttcgattag ggtaaaccta
     5221 aataatacat aaattatatt acaatatgtt tattatagag gctaaagtat cttagaaatt
     5281 tggatagtag gtttttcggg aataaaagta tagttggctt taatatataa ttaagctaag
     5341 ctatcattta tatttactcg attataattc gcacttcaac ttttttataa cgtaaaaatt
     5401 aaaacgtagt tttatttatc tttagtaata tctttttaaa cactacagtt tcttcattag
     5461 ttaaattagc gaagtaatga ttattaagtt gataggcttt cttttctaat gttggccata
     5521 agtcttttgc tttatttgta ctgtttatta gcacagttct tctattttca ttatcagttt
     5581 gacgtgttat tagatccttt ttggacatct tatctaatat tccagatatt gttgaaggtt
     5641 ctaaatttaa agtattacca atcgctttag gtgtattcaa attactatta atcaagcaat
     5701 tcaaaacacc atattctgct ggagtaatat tatagggact taataaactt ttaaaatgtt
     5761 taaaaactct attctgagaa gtactcaaca aataatttat acaattatct aattccacaa
     5821 ctaaaacctc tcttaaatca ttagtattga ctcgcttatc atatcatgat aagttttgta
     5881 ggaaaatagt tttgcaacaa aaacatactt ctacaaaaca attgtgaaag aaggctaata
     5941 aatgttcaat gcggtagtag tgagaaaaag tgcatcaaat gttagttata aagtagaaaa
     6001 agttgatgaa tctcatttat cgaagggtga tgtattagta aaagtggtat actcttcaat
     6061 aaattataag gatatgctag ctttacaata taaaggcggt gtaattagag attatcctat
     6121 gattcctggt atagattttg caggtattgt tgaatcaagt tcgaatgata agtttaaaga
     6181 aggcgataat gtattagtta ctggttatga tgttggcgtg acacatacag gtggattttc
     6241 agaatatgca caagtgcctg gagagtggat tgttcctatt ccccaagaaa tgtctattaa
     6301 agagtctatg gtatatggca cagccggttt tacggcagca ttatcaattt ttgaactaga
     6361 aaaatatgga atgaatatca aatcacaacc tgaaatttta gtgtctgggg ctacaggtgg
     6421 tgtgggcagt gttgctatac aaattttaca taaaattgga tatgaaaata tcactgctat
     6481 cgtaagaaaa gaaaagcaaa tagaaatcgc aaaaaaatta ggtgcaaaac aagtaataat
     6541 aattgaagat gataataaac cgcttaaaaa aggtattttt gattatttct tagatacagt
     6601 ggggagtaat gtaacattat acggtttaaa acgtctaaat tatcaaggtg ttgctactgt
     6661 atgtggaaat gtagcaggaa attcacttaa cgctaatata ttaccgttta ttcttagagg
     6721 tattaaatta attggcattg attctgtata tgttgatcat aatatccgtg aagatatatg
     6781 gagtaagtta gcaaatgaat ggaatatagg aaattcactt ctatacaatg aaattggatt
     6841 tgaagaattc tataaaacga tagatcagct tttaaaagga caacatttag gaagaactat
     6901 tttaaaagta taagtgtgag tattcattaa tacatttact attaattcaa tcattttgca
     6961 tttctctact aaccagtgtt ataatttata taacgaaaat gattaacaat taatgcacca
     7021 atccccacac tattgcagta gtgtggggat tttttcgatg ctggcttata aagtcgccta
     7081 ttaattattt gttgcgttta ctaagccaat aagtataata cgcaacagca cagccactga
     7141 tgactggtgc tatgatgtga acgaaaatga ttagcaaagt aatgcacctc ctctcttcgt
     7201 caattgacgc ctgagagata ggcgactcta ttattatacc atctgtgaat atacaagcat
     7261 atctacttcc gataagtcgt cttatgttat ataactttgt tttttatagt atcaagacaa
     7321 gaagaaactc gtttcaaaaa ccctcatgat ttttagagaa aaactctctt atttcatcgt
     7381 ctttaataaa ataatatgcc atctttccat ctttataaaa atcaagtacc tcatttttat
     7441 ataaaagcct taaatgatgt gaagttgaag caacactcat tttcaatatc aaagaaatat
     7501 cacaaacaca caactcatct tctttaatca aagataatat gattttcaat ttcttctcat
     7561 cacaaatttt ttctaaaatg ttaagtaatt tcttagattt atcatcttct aaaaaactta
     7621 aagcattgtt aactttatcc tcatgtacac agatcacatc acaagtattt tcataactca
     7681 tataaaatca caccctaaat taaaatataa atcctaaaat tgtttgaata gtgtcatttt
     7741 caataataat aaataaacct aaagctatat aaataatagc cataatccaa cgactaaatt
     7801 tctcaacaat ttctccaatt cctggaatat tagctaattt ttgtgcagta aacaccaaga
     7861 agaaaattaa aattaaaaag acaaacagag taagtaataa attagtaaca cttaatgtca
     7921 caaaatacgg aacaaataaa ccaatattat cggcgccaca acttgctatc gtaacaattg
     7981 caaccgtacc aactaattta gacaatcctt tttcattcaa ttctttttta gctctctttt
     8041 ctccttcaca atcatcataa atagccactt taattccaag ataaattggt attaaaccca
     8101 ataaacctaa tatccacttt tctggaacat aatttaatac aaaagctaaa aacaaactaa
     8161 ctaatattaa aataatagac cctaaatatt gaccaacata aatatctcta tattcttttc
     8221 tagtctttgc tctagcaaaa aatattaata aaattactaa taaatcaact gctgtagcaa
     8281 tataaagaac agcagcagca acaaccgttt gaatcataat acacctcatt caaaaatatt
     8341 tttgaatgta ttataactta tatttttaaa taatcatagg tg
//
LOCUS       DAKTGS010000125         8334 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.125, whole genome shotgun sequence.
ACCESSION   DAKTGS010000125 DAKTGS010000000
VERSION     DAKTGS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.125"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            76..957
                     /locus_tag="O0286_002277"
     CDS             76..957
                     /locus_tag="O0286_002277"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCY0170635.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            975..2249
                     /locus_tag="O0286_002278"
     CDS             975..2249
                     /locus_tag="O0286_002278"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCY0170636.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            2251..3351
                     /gene="carA"
                     /locus_tag="O0286_002279"
     CDS             2251..3351
                     /gene="carA"
                     /locus_tag="O0286_002279"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCY0170637.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            3344..6517
                     /gene="carB"
                     /locus_tag="O0286_002280"
     CDS             3344..6517
                     /gene="carB"
                     /locus_tag="O0286_002280"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCY0170638.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            6627..7319
                     /gene="pyrF"
                     /locus_tag="O0286_002281"
     CDS             6627..7319
                     /gene="pyrF"
                     /locus_tag="O0286_002281"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCY0170639.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            7319..7930
                     /locus_tag="O0286_002282"
     CDS             7319..7930
                     /locus_tag="O0286_002282"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCY0170640.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            7960..8172
                     /locus_tag="O0286_002283"
     CDS             7960..8172
                     /locus_tag="O0286_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170641.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
BASE COUNT         3055 a         1236 c         1626 g         2417 t
ORIGIN      
        1 attattttga acttaatctt acctaaagag aaaaaacaaa acaattaaga tttacaaatt
       61 aaggagggcg cttttatgaa tcatttatta tcaatggaac atttatctac agatcaaata
      121 tacaaactta tccaaaaggc aagtcaattt aaatctggtg aacgtcaact accaaacttt
      181 gaagggaaat atgtcgcaaa tttattcttt gaaaattcta ctcgaacaaa atgtagtttt
      241 gaaatggcag aacttaagct agggttaaaa acgattagct ttgaaacatc aacatcatct
      301 gtttcaaaag gtgaatcttt atatgacaca tgtaaaactt tagaaagtat tggctgtgat
      361 ttattagtca ttagacatcc gtttaataac tactatgaaa aattagcgaa tattaacatc
      421 ccaattgcga atgctggtga tggtagtgga caacatccaa cacaaagttt acttgattta
      481 atgacgatat atgaagaata tggatatttt gaaggcttga atgtattgat ttgtggagac
      541 attaaaaatt cacgtgtcgc acgtagtaat taccatagtt taaaagcatt aggtgcaaac
      601 gtaatgttta atagcccaaa tgcttggatt gatgattctt tagaagcacc ttatgtaaat
      661 atagatgatg ttatagaaac agtagatata gttatgttat taagaattca acatgaaagg
      721 catgggcttg cagaagaaac tagatttgca gcagatgatt atcatcaaaa gcatggctta
      781 aatgaagtac gctataacaa attacaagaa catgctattg ttatgcatcc ggcacctgtg
      841 aatagaggag tagaaataca aagcgattta gtagaagctt caaaatcaag aatttttaag
      901 caaatggaaa atggcgttta cttaagaatg gcagtcattg atgaattatt aaaataggta
      961 aggggacgaa aatgatgaaa ttaattaaaa acggtaaagt attacaaaat ggcgaattac
     1021 aacaagcaga tattttaatt gatggtaagg taattaaaca aattgcacct gcaattgaac
     1081 caagcaatgg tgttgacatc atagatgcga aaggtcactt tgtgtcacct ggatttgtcg
     1141 atgttcatgt tcatttacgt gaacctggtg gtgaatataa agagacaatt gaaactggta
     1201 ctaaagctgc tgctagaggc ggatttacaa ctgtatgtcc aatgcctaac acaagaccgg
     1261 taccagattc tgtagaacat tttgaagctt tacaaaaatt aatcgatgac aatgctcaag
     1321 tacgtgtatt accttatgct tcaattacaa cacgtcaatt aggtaaagaa ttggttgatt
     1381 tcccagcact agtaaaagaa ggtgcctttg cgtttacaga tgacggtgta ggagtacaaa
     1441 ctgcaagcat gatgtatgaa ggcatgattg aagctgcaaa agtaaacaaa gccatcgtag
     1501 cacactgtga agataattca ttaatctatg gtggtgcaat gcatgaaggg aaacgcagta
     1561 aagagttagg tataccaggt attccaaaca tttgtgaatc tgttcaaatc gcaagagatg
     1621 tactattagc tgaagcagca ggttgtcatt atcatgtatg tcatgtttct actaaagaaa
     1681 gtgttagagt cattcgtgac gctaaacgcg caggcattca tgttacagct gaagttacac
     1741 cacaccattt attgttaaca gaagatgata ttcctggtaa taatgccatt tataaaatga
     1801 atccaccatt gagaagtact gaagatagag aggctttgtt agaagggtta ctagacggta
     1861 caattgactg tatcgcaaca gaccatgcac cacatgcacg tgatgaaaaa gcacaaccaa
     1921 tggaaaaagc accattcgga attgttggta gtgaaacagc attcccatta ttatatacgc
     1981 attttgtaaa aaatggtgat tggacattac aacaattagt agattactta acaattaaac
     2041 catgtgagac atttaattta gaatacggca cattaaaaga aaatggttat gcagatttaa
     2101 caatcattga tttagatagt gaacaagaaa ttaaaggaga agatttctta tcaaaagcag
     2161 ataatacacc atttatcggc tataaagttt atggaaatcc gatcttaaca atggttgaag
     2221 gcgaagttaa atttgagggg gataaataat atgcaaagca aacgttatct agtgttagaa
     2281 gacggttctt tttacgaagg ctaccgttta ggatctgata acttaactgt aggagaaatt
     2341 gtatttaata cagcgatgac aggttatcaa gaaactattt cagatccatc atatacaggt
     2401 cagatcatta cttttacgta tccattaatc ggtaattatg gtatcaatag agacgatttt
     2461 gaatcattag tacctacatt aaacggtatt gtagtgaaag aagcgagtgc gcatccaagt
     2521 aattttagac agcaaaagac acttcatgac gttttagaat tgcatcaaat tccagggatt
     2581 gcaggtgttg atacaagaag tattacgcgt aaaattcgac aacacggtgt gttaaaagct
     2641 ggttttactg atcgaaaaga agatattgat caacttgtca aacatttaca acaagtagaa
     2701 ttacctaaaa acgaagtaga aatcgtttcg actaaaacac cgtatgtttc gacaggtaag
     2761 gatctaagtg tcgtacttgt agactttggt aagaagcaaa atattgttcg agaattaaac
     2821 gtcagaggtt gtaacgtcac agttgtacca tatacaacta ctgccgaaga aattttagca
     2881 atggctccag atggcgttat gctatcaaac ggaccaggta atcctgaagt tgtagaatgt
     2941 gcgattccaa tgattcaagg aattttaggg aaaattccgt tctttggtat ctgtctagga
     3001 catcaacttt ttgcattatc tcaaggagca agctcattta aaatgaagtt tggtcatcgt
     3061 ggtgcgaacc atccagttaa aaatttagag actggaaaag ttgatattac gagtcaaaac
     3121 catggatatg caatagatat agattcgtta aaaagtactg atttagaagt tactcatctt
     3181 gcattaaatg atggtactgt agaaggttta aaacataaaa cattaccagc attttctgtt
     3241 caataccatc ctgaagcaaa tccaggaccg tcagattcaa actatctatt tgatgatttt
     3301 gtagcaatga tgactaattt taaggaaaag gagcgtcata tcaatgccta aacgtaatga
     3361 tatcaaaaca attttagtaa tagggtctgg gccaattatc ataggtcaag cagctgaatt
     3421 tgattatgct ggaacacaag catgtctagc tttaaaagaa gagggatatc gagttattct
     3481 tgtaaattca aatccagcga caatcatgac tgataaggaa attgcggata aagtatatat
     3541 cgaaccgtta actcatgatt ttatagcgcg aattatacgt aaagagcaac ctgacgcttt
     3601 acttccaact ttaggtggtc aaacaggttt aaacatggcg attcaactac acgaaagtgg
     3661 tgtgcttcaa gataataacg tccaattatt aggaactgag ctaacatcaa ttcaacaagc
     3721 agaagaccgt gaaatgttta gaacattaat gaatgattta aacgttcctg taccagagag
     3781 tgacattgta aatacagtag agcaagcctt taaattcaaa gagcaagtgg gatacccgct
     3841 aattgttaga ccggcattta cgatgggtgg taccggaggc ggtatttgtc ataatgatga
     3901 agaattacat gaaatcgtct caaatggtct tcattatagt ccagcaacgc aatgtttatt
     3961 agaaaaatct atcgcaggtt ttaaagaaat cgaatacgaa gtaatgcgtg ataaaaacga
     4021 taatgccatc gttgtatgta acatggaaaa tattgatcca gttggtattc atacaggcga
     4081 ttcaattgtt gtggctccta gtcaaacatt atcagatgtt gagtatcaaa tgttacgtga
     4141 tgtttcatta aaagttattc gagctttagg tatcgaaggt ggttgtaatg ttcaattagc
     4201 attagatccc cattcattcg attattatat tatagaagta aatccgcgtg tatcacgttc
     4261 atcagcgtta gcttcaaaag caacaggata tcctattgca aaattagctg ctaaaatcgc
     4321 ggttggtcta acattagatg aaatgttaaa tccaattaca ggaacatctt atgcagcgtt
     4381 tgaaccaact ttagactatg tgatttcaaa aataccaaga tttccttttg ataaatttga
     4441 aaaaggagaa cgagagcttg gcacacaaat gaaagcaaca ggtgaagtta tggccattgg
     4501 tcgaacttac gaagaatcat tgttaaaagc aattcgatca cttgagtatg gtgtgcatca
     4561 cttaggatta ccaaatggtg aaagcttcga tcttgattat attaaagaac gtatttcaca
     4621 ccaagatgat gaacgattat ttttcatcgg cgaagcaatt agaagaggca caacattaga
     4681 agaaattcat aatatgactc agattgatta cttcttctta cacaagttcc aaaacattat
     4741 tgatattgag catcaattaa aagagcatca aggtgattta gaatatctta aatatgcaaa
     4801 agattatgga tttagtgata aaacaatagc gcatcgcttt aatatgacgg aagaagaagt
     4861 atatcaattg cgtatggaaa atgatattaa acctgtttac aagatggttg atacttgcgc
     4921 agctgaattt gaatcttcaa caccatatta ttatggtaca tacgaaactg aaaatgaatc
     4981 catagttact gacaaagaaa aaatcttagt attaggctct ggaccaattc gaatcggcca
     5041 aggtgtagaa tttgactatg cgacagttca cgccgtttgg gcaattcaaa aagcagggta
     5101 cgaagcgata attgtgaata acaatccaga aacagtttca acagacttct caatttctga
     5161 caaattatac tttgaacctt taactgaaga agatgtgatg aatatcatta atttagaaaa
     5221 acctaaaggt gtcgttgtac aatttggagg acaaacagcg attaatttag cagacaaatt
     5281 ggctaaacat ggtgttaaaa tacttggtac ttcactagaa aatctaaatc gtgctgaaga
     5341 tagaaaagaa tttgaagcac tattaagaaa aattaacgtg ccacagccac aagggaaaac
     5401 agctacatca cctgaggaag cattagcgaa tgctgcagaa atcggatatc cggttgtagt
     5461 aagaccttct tatgtattag gtggtcgcgc aatggaaatt gtagacaatg acaaagagtt
     5521 agaaaactat atgacccagg ctgtaaaagc gagtccggaa catccggtac tagtcgatag
     5581 atatttaact ggtaaagaaa ttgaagttga tgcgatttgt gatggagaaa cggtcattat
     5641 tccaggaatc atggaacata ttgaacgtgc tggtgtgcat agtggtgact caatcgctgt
     5701 atatccacca caaactttga cagaagacga gttagcaaca cttgaggact atactataaa
     5761 attagctaaa ggtttaaaca tcattggctt aatcaacatt caattcgtta tagctcacga
     5821 tggtgtgtat gttttagaag taaatccacg ttctagtaga acggtaccat tcttaagtaa
     5881 aattactgat attccaatgg cacaattagc tatgcgagca atcattgggg aaaaactaac
     5941 agatatgggt tatcaagaag gggttcaacc atatgctgag ggtgtctttg tgaaagcacc
     6001 agtatttagt tttaataaat tgaaaaatgt tgatattact ttaggacctg aaatgaagtc
     6061 aacaggtgaa gtgatgggga aagatactac attagaaaag gcgttattca aagggttaac
     6121 aggtagtggc gttgaagtta aagatcacgg tacagtatta atgaccgtca gtgacaaaga
     6181 taaagaggaa gttgttaaat tggcacaacg cttaaatgaa gttggctata aaattttagc
     6241 aacgtctgga acagctaata aattagctga gtatgacata cctgcagaag tagtaggcaa
     6301 aattggtggc gaaaatgatt tattaacacg tattcaaaat ggtgatgttc aaatcgttat
     6361 aaatacaatg actaaaggta aagaagtaga aagggatggc ttccaaatta gacgtactac
     6421 agttgaaaat ggtattccat gtttgacatc tttagataca gctaatgcct taacgaatgt
     6481 aattgaaagt atgacattta caatgcgtca aatgtaaatc aatcaaactg tatcggtggg
     6541 gctgtaatta accatttact taaagaagtt tatattacag cctcattatt ttaatgaatt
     6601 tcttaatata aagggagaca tatatgatga aagatttacc aattattgca ttagattttg
     6661 aatcaaaaga aaaagtaaat caatttttag atttatttga tgaatcatta ttcgtaaaag
     6721 taggtatgga acttttttat caagaaggtc ctcaattaat taatgagata aaagaaagag
     6781 gccatgatgt atttttagat ttaaaactgc atgatattcc taatacagtt ggtaaggcga
     6841 tggaaggact agctaaattg aatgttgatc tggtaaatgt tcatgctgct ggtggcgtaa
     6901 aaatgatgtc tgaggccatt aaaggattaa gaaaacataa tcaagataca aaaattattg
     6961 cagtaacaca gcttacgtca acaacagaag acatgttacg acacgaacaa aatatacaaa
     7021 catcgattga agaggccgtt ttaaattatg ccaagttagc aaatgcagct ggtttagatg
     7081 gcgttgtttg ttcacctctt gaaagtcgta tgttgactga aaagttaggt acatcatttt
     7141 taaaagtaac accaggtatt agacctaaag gtgcatctca aaatgaccaa caccgtatta
     7201 cgacaccgga agaagcaaga cagcttggtt cgacgcatat tgtagtcggt agaccgatta
     7261 cacaaagtga caatccagtc gaaagttatc ataaaattaa agaaagttgg ttagtataat
     7321 ggctaaagaa attgcaaaat cattattaga tattgaagct gtaacattat caccaaatga
     7381 tttatataca tggagttcag gtattaaatc accgatttac tgtgataacc gtgttacgtt
     7441 aggttatcct ttagttcgag gcgcaatccg cgatggttta attaacttaa ttaaagaaca
     7501 ctttcctgaa gtagaagtta tttctggtac tgcaacagct ggtattccac atgcagcttt
     7561 tattgctgaa aaattaaaat taccaatgaa ttatgttcgt tcatcaaata agagtcatgg
     7621 taagcaaaat caaatcgaag gtgctaaaag tgaaggtaaa aaagtagttg tgatagaaga
     7681 tttaatttcg acagggggat cttcagtcac agcagttgaa gccttaaaac tagcaggtgc
     7741 agaagtatta ggtgttgtag ctatctttac ttacggtttg aaaaaagcag atgatacatt
     7801 tagcaatatt caactacctt tttacacttt aagtgattac aatgaattaa ttgaagtagc
     7861 tgaaaatgaa ggtaaaattt ctagtgaaga tatccaaaca ttagttgaat ggagagacaa
     7921 cttagcataa tatagacact agaaggagga attcaacaaa tgaatgacaa aacatctaat
     7981 gatttatatg ggaagataaa acattgtaac gaatttatca atcattcaaa tgattccaat
     8041 ctatctagta gtcacgatgt cgacgaaagt tcaacgaagc aaaaacatat aaaaaataaa
     8101 acaactatag atcataatga tgatttattt aaacatgtaa aggatatatt acgtaaacaa
     8161 gaacaaattt aaaaacaaaa aagctatagt gtttaatatc aggggctgct taaccgcatc
     8221 cgaatattac aaacactata gcttttatta tataaaaaat ttattagcgg ataattaact
     8281 ttattaaaat ccaaccaatt aagaatacga gtaataggac taatactgga atca
//
LOCUS       DAKTGS010000126         8198 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.126, whole genome shotgun sequence.
ACCESSION   DAKTGS010000126 DAKTGS010000000
VERSION     DAKTGS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.126"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..184
                     /locus_tag="O0286_002284"
     CDS             <1..184
                     /locus_tag="O0286_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCY0170642.1"
                     /translation="LRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKLQTHRM
                     GDVIDGRNIEKIEVNL"
     gene            206..1714
                     /locus_tag="O0286_002285"
     CDS             206..1714
                     /locus_tag="O0286_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCY0170643.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(1817..2125)
                     /gene="spn"
                     /locus_tag="O0286_002286"
     CDS             complement(1817..2125)
                     /gene="spn"
                     /locus_tag="O0286_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCY0170644.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            2493..3266
                     /gene="lpl1"
                     /locus_tag="O0286_002287"
     CDS             2493..3266
                     /gene="lpl1"
                     /locus_tag="O0286_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCY0170645.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            3297..4097
                     /gene="lpl2"
                     /locus_tag="O0286_002288"
     CDS             3297..4097
                     /gene="lpl2"
                     /locus_tag="O0286_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HCY0170646.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            4116..4923
                     /locus_tag="O0286_002289"
                     /pseudo
     CDS             4116..4923
                     /locus_tag="O0286_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            4972..5772
                     /gene="lpl4"
                     /locus_tag="O0286_002290"
     CDS             4972..5772
                     /gene="lpl4"
                     /locus_tag="O0286_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCY0170647.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDKKLKQKIENFK
                     FFSQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVL
                     KMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKTNKDDE"
     gene            5791..6561
                     /gene="lpl5"
                     /locus_tag="O0286_002291"
     CDS             5791..6561
                     /gene="lpl5"
                     /locus_tag="O0286_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCY0170648.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            6593..7384
                     /gene="lpl6"
                     /locus_tag="O0286_002292"
     CDS             6593..7384
                     /gene="lpl6"
                     /locus_tag="O0286_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HCY0170649.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            7454..>8198
                     /gene="lpl7"
                     /locus_tag="O0286_002293"
     CDS             7454..>8198
                     /gene="lpl7"
                     /locus_tag="O0286_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HCY0170650.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTD"
BASE COUNT         3412 a          901 c         1544 g         2341 t
ORIGIN      
        1 attaagaaag catttaattg ataaacataa cctttataag acagaaccta aagacagtaa
       61 aattcgaatt actatgaaag atggtgggtt ctacacattt gaattgaata aaaagttaca
      121 aacacaccgt atgggtgatg ttattgatgg cagaaatata gaaaaaattg aagtgaattt
      181 ataaaattat tcgagggagc atatcatgag ggaaaatttt aagttacgta aaatgaaagt
      241 cggtttagta tctgttgcaa ttacaatgtt atatattatg acaaacggac aagcagaagc
      301 atctgaaaat caaaacgctt taatctctaa tataaatgta gacaatcagg aaaaacagaa
      361 taatgtaaat caagctgttc agcctcaaaa taatactaat gaaacatcaa aagtaccggc
      421 taattttgtc aaattgaatg atattaaacc aggtgatact tctatacaag gaacaacttt
      481 accaaatcaa tttatactat taactattga taaaaaagat gtgagctcag ttgaagattc
      541 tgacagcagc tttgttatgt ctgataaaga tgggaatttt aagtatgact taaatggtcg
      601 caaaattgtt cataatcaag aaattgaagt gtcttcatca gatccctatt taggtgacga
      661 tgaagaagat gaagaagtag aagaaacttc aactgaagaa gttggtgctg aggaagaaag
      721 tacagaagct aaagctacat atacaacacc gcgatatgaa aaagcgtatg aaataccgaa
      781 agaacagcta aaagaaaaag atggacatca ccaagttttt atcgaaccta ttactgaagg
      841 ttcaggtatt attaaaggcc atacctctgt aaaaggtaaa gttgctctat ctattaataa
      901 taaatttatt aactttgaga caaatgctaa tggtggtcca aataaagaag aagcgaaatc
      961 tggatcagaa ggaatctgga tgcctattga tgacaaagga tactttaatt ttgacttcaa
     1021 aacgaaacgt ttcgatgatt tagagttaaa gaaaaatgat gagatctcat taacatttgc
     1081 acctgatgac gaagatgagg cattgaagtc attaattttc aaaactaagg taacgagttt
     1141 agaagatatt gataaagcag aaactaaata tgaccatact aaagtggaaa aagtaaaagt
     1201 attgaaagat gttaaagaag atttacatgt agatgaaatt tacggaagct tatatcatac
     1261 agaaaaaggt aaaggtattc ttgataaaga aggtactaaa gtaattaaag gtaagactaa
     1321 attcgcaaat gcagttgtga aggtagactc tgaactaggt gaaggtcaag aattccctga
     1381 tttgcaagtc gatgaaaaag gtgaattcag ctttgatgta gatcatgctg gatttagatt
     1441 acaaaatgga gaaacactaa acttcacagt agttgatcct attacaggtg aattattaag
     1501 tggaaatttt gtttctaaaa acatagatat ttatgaatct cctgaagaaa aagcagatcg
     1561 tgagtttgat gaaagaatgg aaaacacacc tgcatatcat aaattacatg gtgataaaat
     1621 tgtcggctac gatactaacg gattcccgat tacctggttt tatccattag gtgaaaagaa
     1681 agttgaacgt aaggcaccaa aattagaaaa ataattaaat aaaacagctt aatgatgtaa
     1741 tgaaattagt gagttaatca ctgacttcta cgtcattgag ctgttttttg tgctttgtta
     1801 caaagcatta ttgaatttat tttacgtgtt catattttga aacatcaaag ccgtcttgtt
     1861 tagctttgtt gataatgtct ttgattgaat gtagtccttt atcggcgaag tatgatctta
     1921 agttgtcttt tgtagcttgg tcagcattct tatctaataa cacatcaata taacttaatt
     1981 catgttctaa gaagtttgca tcatcatgta gtacgagtcc attttgagaa taaactttcg
     2041 catctgcttg attaccatat ccaacaacgc cagttgctaa aacacctacc attgccgtag
     2101 ctactaaaac ctttttaaat ttcatatcta tcactcctct aaaaattgta actccatcat
     2161 aacactgaat attaagaaaa ttacgtttat taagtcgatt taataatttt taataaatag
     2221 ttaaagtgac aaatattgtt taaatgcaat taatctttaa tacgatgctt gaggattttt
     2281 cctaataaaa ccttgatttc aaaaagggtt taaatcaaat gaaacaataa taaaaaaatg
     2341 acgcaatata ataataagta caaatttaat taagaaatta aattgattgt atatgtatat
     2401 tttggtaacg taaaagagaa atatacaaaa taattaatta tttatatgaa aagagaatat
     2461 aaatgaagta taaaacagag agacgtgaag cgatgggata tttaaaaagg tttgcattgt
     2521 acataagcgt tatgatttta atatttgcga tagcaggttg tggcaaaggt aatgaaacaa
     2581 aagaagattc aaaggaagaa caaatcaaaa agagctttgc gataacatta gatatgtatc
     2641 caattaagaa tctcgaggac ttatatgaca aagaaggata tcgagatagc gaatttaaaa
     2701 aaggtgacaa agggatgtgg acgatatata cagatttcgc caaaagtaat aaaccgggtg
     2761 aattggatga tgaaggtatg gttttaaatc tggataggaa tactagaacc gctaaaggcc
     2821 attattttgt tactacattt taccggaatg gtaaactacc agatgaaaag aattataaaa
     2881 ttgaaatgaa aaataataag attattttat tagatgaagt aaaagatgat aagctcaagc
     2941 agaaaataga aaactttaaa tttttcggtc aatatgcaaa tcttaaagaa ttgagaaaat
     3001 ataataatgg tgatgtttca attaatgaaa atgttcctag ttatgatgtt gaatacaaaa
     3061 tgagcaataa agatgaaata gttaaggaat taagaagtcg ttataatatt tcgactgaaa
     3121 aatcacctat attaaaaatg catattgatg gggacctgaa aggcagttct gtaggttata
     3181 gaaagttaga aattgacttt tcaaaacgtg aaaacagcaa attatcagtc attgaatttt
     3241 taagttataa accagcgaaa aaatagtatt gataaggaat attagggtgt gaaaaaatgc
     3301 gatatctaaa aaagcttgca tggttcataa gtgttattat tttgggcatt tttataatag
     3361 gttgtgatag ttcaagcgat actggggaaa aagcaaaaga agattcaaag gaagaacaaa
     3421 tcaaaaagag ctttgcgaaa acattagata tgtatccaat taagaatctc gaggacttat
     3481 atgacaaaga aggatatcga gatagcgaat ttaaaaaagg tgacaaaggg atgtggatga
     3541 tatatacaga tttcgccaaa agtaataaac cgggtgtatt agataatgaa ggtatgattt
     3601 taaatttgga tagaaataca cgtacggcca agggatatta ttttgtagat actatatatg
     3661 acaatcatga aaactcttat agtaaaaatt atagagttga gatgaaaaac aataaaatta
     3721 ttttattaga caaggtggaa gatcaaaaac ttaaagaaag aatagaaaac tttaaatttt
     3781 tcggacaata tgccgatttc aagagtttga aaagttacaa caatggcgac gtttcaatta
     3841 atagtaatgt tccaagttat gacgcgaaat ttaaaatgag taataaagat gaaaatgtta
     3901 agcaattaag aagtcgttat aacattccta ctgaaaaagc tccaatatta aaaatgcata
     3961 ttgatgggga cttaaaaggc agttccgttg gatataaaaa gttagaaata gacttttcaa
     4021 aagaagaaaa tagcgaatta tcagtagtcg attcattaaa ttttcagcct gccaaaaaaa
     4081 ataaagatga tgaatgatag ggaggatgtg aaacaatgtg gtcaataaaa agaattggaa
     4141 tgtacacaaa tgttataatt ttgagcgttt ttataatagg ttgtgatagt tcaagcaata
     4201 atgcagaaaa tcaaagagaa gactcaaaag aagaacaaat caaaaagagc tttgcgaaaa
     4261 cgttagatat gtaccctatt aagaatctcg aggatttata tgacaaagaa ggatatcgtg
     4321 atggtgagtt tgaaaaaggc gataagggga cgtgggtatt gtattcagct atggtatcac
     4381 aaccaaaagg cgagagttta aaatcacgag gaatgatatt aaagttagat agaaataaga
     4441 gaactgctaa aggaagttat attattagag aattgaaaga agataaaaat catgatgttc
     4501 aaaaaaatga aaagaaatat ccagtgaaat tggtgaataa taggatagtt ttgataaaag
     4561 atgttaaaga caaaaagtta aaaaaatgaa atagagtcgt ttgaattatt ttcacaatat
     4621 ggaaacttta atcattttga tcggaatgag attactaata tttcatataa tcctaatgct
     4681 cccaattact ctgcagaata taaaatgaag aaaaatgaca gaaacattca acagttgaaa
     4741 aagagattta atctaaaaac tagcaagaca ccaaaattat tgtttaaggg atctggagat
     4801 ataaaggggt cttctgtagg atataaggaa atagaaatca tatttagtag aagtaaagaa
     4861 gaaagcattt attatgttga cagcgttgag ttcgttccaa gtaacaaaat aacaaaaagt
     4921 tgatattagt caacaaaagc aaatattgag aataaagaga gttgtgaaat gatgggttat
     4981 ttaaaaagac ttgtattgta tatagttatt atggttatga gtgtttttat aataggttgt
     5041 gataaatcaa gcgatactgc agaaaaatca aaagaagact cgaaagaaac acaaatcaaa
     5101 aagagttttg cgaaaacgtt agatatgtat tcaattaaga atctcgagga actatatgac
     5161 aaagaaggct atcgagatgg cgaatttgaa aaaggtgaca aagggatgtg gacgatatat
     5221 acagatttcg ccaaaagtaa taaaccgggt gaactaagta atgaaggcat ggtcttgtac
     5281 ttagatagaa acacacgcac agctaagggg tactactttg tcaggacgtt ttatcgcaag
     5341 gataaattac ctgatagaaa aaattataaa gttgaaatga aaaataataa aatcatttta
     5401 ttagataaag tagaagataa aaagcttaaa caaaaaatag aaaattttaa atttttcagt
     5461 caatatgcaa atcttaaaga attgaaaaac tacagcaatg gtgatgtctc aattaatgag
     5521 aatgttccaa gttatgacgt gaaatataaa atgagtaata aagatgaaaa tgttaagcaa
     5581 ttaagaagtc gttataatat tcctactgat aaatcaccgg tattaaaaat gcatattgat
     5641 ggtaatttga aaggaagttc cgttggagat aggaagttag aaattgattt ttcgaaacgt
     5701 gaaaatagcc atttatcagt aatagattct ttggattacc agccagcgaa aactaataaa
     5761 gatgatgaat aatgaggatg gtgtgtaaca atgaagtcta taaaaaggat tggattgtgc
     5821 ataagtctgt tgattttaag catttttgta acatcttgcg atggtgataa taagatcact
     5881 ggagattcaa aagaaacaca aatcaaaaag agcttttcga aaacgttaga tatgtatcca
     5941 attaagaatc tcgaggactt atatgataaa gaaggatacc gtgatggcga atttaaaaag
     6001 ggcgacaaag gaatgtggac tatatataca gattttgcta aaggcaataa atcagacgaa
     6061 ttggatgatg aaggtatggt tttaaatctg gatagaaata ctcgaacggc taagggatat
     6121 tattttgtta agaaatttta tgaaaaggat aaattacctg atagaaaaaa ttataaagtt
     6181 gaaatgaaaa ataataaaat tatcttatta gacaaggtag aagatccaaa tctaaaaaag
     6241 agaatagaaa actttaaatt tttcggacaa tatgcaaatt ttaaggattt ggaaaattac
     6301 aacaatggcg acgtgtcaat aaattggaat gttccaagtt atgacgtgga atataaaatg
     6361 agcaataaag atgaaaatgt taaacaatta agaagtcgtt ataacattcc tactgataaa
     6421 gctccaatgt taaaaatgca tattgacggg gacttaaaag gtagttctgt tggatataaa
     6481 aggttagaaa tagatttttc aaaagaaggt agggatattt cagtcattga ttatttaagt
     6541 tataagccag cgaaaaaata gtactgataa ttaaatatta gggtgtgaag gaatggaata
     6601 tctaaaaagg cttgcattgt taataagtgt tattattttg accattttta taatgggttg
     6661 tgatagtcaa agcgatactg cagaaaatcc aaaagaaggt tcaaaagaag cacaaattaa
     6721 aaagagtttt tcgaaaacgt tagatatgta tccaattaag aatctcgagg atttttatgg
     6781 caaagaagga tatcgagatg gcgaatttaa aaaagatgat aaaggtactt ggctaattag
     6841 atccgaaata gttaaacagc caaagggcaa agtgatgaaa acaagaggta tgcaattata
     6901 tattaataga aataccgaaa cagccaaagg tttctttgtt ttgaaagaaa taagtgaaaa
     6961 taataatcgt gtaaataaag ataaggagga aaaatacgaa gtgaaaatgg taggaaataa
     7021 aattattcct actgaacaaa ttaatgacga gaaaataaaa aaagaaattg aaaacttcaa
     7081 gttttttgtg caatatggaa actttaaaaa tttcgaaaaa tacaacaatg gtgagttttc
     7141 atataatcct gaagcaccaa tttattcggc taaatatcaa ttacacaacg atgattacaa
     7201 tgtaaggcaa ctacgtaaaa gatatgacat ttcaacaaaa gaaacaccga agttactttt
     7261 gaaaggtgga ggagatttaa aaaattcctc agttggtcaa aacgatattg aatttacttt
     7321 tgttgaaaga aaaggtgaga atatttattt taacgatagt gttgaattca taccaagtaa
     7381 gtaaagttaa attaaaatgt gtggttagat gaaatgatca aatatcaaaa aatgtgcaaa
     7441 gagatgtgac aagatgaagt gttttcagaa attatacata tttatattaa ttttaatcgt
     7501 attaatggca ggatgcgaaa gtaataagat cactggagat tcgaaagaaa cacagatcaa
     7561 aaagagcttt gcgaaaacat tagatgtata ccctacgaaa aatctagaag atttttatga
     7621 caaagaagga tatcgagatg gcgaatttaa aaagggtgac aaagggaagt gggttattag
     7681 atctgaaatg acaacagaac tgaaaaatga aaatatggta tctaaaggta tggtcatacg
     7741 tttaaataga aatagtagaa catgcactgg tgaatatttt gtcaggatag ttaaagaaga
     7801 cagtgagggc aaggtatata gtgatgaacg aaaatatcca gtgaaaatgg aaaataataa
     7861 aatcattaca ttaaaaccaa tcgatgatga aaaagtaaaa aaagaaattg aagaatttaa
     7921 attcttcgta caatacggga atttcaaaga attggaaaac tataaagacg gagaagtgac
     7981 atataaccca gaagcaccaa tatactctgc acaatatcaa ttgaaaaata gtgattataa
     8041 tgtagaacaa ctacgtaagc gatataatat aacgacgaaa aaagcgccta aattattatt
     8101 gaagggttca ggtaatttaa aaggctcatc agttggatat aaaaatattg aatttacctt
     8161 tgttgaaaat aaggaagaaa atatttactt cacagata
//
LOCUS       DAKTGS010000127         8180 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.127, whole genome shotgun sequence.
ACCESSION   DAKTGS010000127 DAKTGS010000000
VERSION     DAKTGS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.127"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            4..411
                     /locus_tag="O0286_002294"
     CDS             4..411
                     /locus_tag="O0286_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170651.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            461..1087
                     /locus_tag="O0286_002295"
     CDS             461..1087
                     /locus_tag="O0286_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170652.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            1299..2075
                     /locus_tag="O0286_002296"
     CDS             1299..2075
                     /locus_tag="O0286_002296"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCY0170653.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            2219..2341
                     /locus_tag="O0286_002297"
     CDS             2219..2341
                     /locus_tag="O0286_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170654.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            2411..3391
                     /locus_tag="O0286_002298"
     CDS             2411..3391
                     /locus_tag="O0286_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0170655.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            3354..4046
                     /locus_tag="O0286_002299"
     CDS             3354..4046
                     /locus_tag="O0286_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCY0170656.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            4256..5422
                     /locus_tag="O0286_002300"
     CDS             4256..5422
                     /locus_tag="O0286_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCY0170657.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            5403..6641
                     /locus_tag="O0286_002301"
     CDS             5403..6641
                     /locus_tag="O0286_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0170658.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            6631..8061
                     /locus_tag="O0286_002302"
     CDS             6631..8061
                     /locus_tag="O0286_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCY0170659.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         2744 a         1135 c         1497 g         2804 t
ORIGIN      
        1 agtatgagct caattatagg aaaaatagca atttggatag gcatcgtagc tcaaatatat
       61 tttagtgtcg tttttgttag gatgatatct attaatattg ctggaggatc tgattacgaa
      121 acaatttttt tattaggatt aatattggct cttttcactg ttttaccaac catctttact
      181 gcgatttata tggaaagtta ctctgtaatc ggaggtgcac tttttattgt ttatgctatt
      241 attgcactgt gtttatataa tttcctttcg tcaattttat ggctgattgg tggtattttg
      301 ctgatttgga ataaatactc aaaagatgaa tcgacagacg aaaatgaaaa agttgatatt
      361 gaaagtacag agaatcaatt tgaatctaaa gataaaatca ctaaagaata aagagaatat
      421 ttaaggtaaa gtataaattt taaataaatg gggaatagac atggaaaaaa atgtagaaaa
      481 atcattcata aagataggtt tatattttca aatagcttat atagtactca tggctataac
      541 tttatgtggg tttgtaattt gctatggact aattttcggc cttttctatt tattatcagg
      601 tagcagagct gattatttaa tagtaacaat agttatatcg gcaataattt ctatatttgt
      661 aattatactt tcaatcgtac ctgtcatcgt attggcatct gacttattta aagaaaggat
      721 ttcaaaaggt gtcatattaa ttgtattggc tattatcgct ttagtattat gcaactttgt
      781 atctgcaata ctctggtttg tttcagccat atctatttta ggtagaaaaa aattagtagc
      841 tgcagcagat actaccacta ttcaaaaaag taaagggaac gcaaatcaag catcacataa
      901 agacacgtgt aaaaaggaac ttgatagtca agacatgatg gaacatcctg aggttaaaaa
      961 tcccacgact aaaaaccttg aaggatttaa cgaagaaata cataaagatg aagctacaac
     1021 taaagttgtc agtgataaca cggaaccgcc tattgaatca aaagaccatg tctcgaaaaa
     1081 agattgatga caaactaatc gagagactta aaaaaataat attcaacata agaactttta
     1141 aaacgacatt taaacgcatt gccaatcact aatggtagtg cgtttaacta taccttaaat
     1201 atctgaatat tttgttaaat ggagctacct ttgttgtact attcaaatga agaggagtaa
     1261 aatgtaatta aaggaaagaa atttgaggag tgatctttat gacaaacaac aaagtagcat
     1321 tagtaactgg cggagcacaa gggattggtt ttaaaattgc agaacgttta gtggaagatg
     1381 gtttcaaagt agcagttgtt gatttcaatg aagaaggggc aaaagcagct gcacttaaat
     1441 tatcaagtga tggtacaaaa gctattgcta tcaaagcaga tgtatcaaac cgtgatgatg
     1501 tatttaacgc agtaagacaa actgccgcgc aatttggcga tttccatgtc atggttaaca
     1561 atgccggcct tggaccaaca acaccaatcg atacaattac tgaagaacag tttaaaacag
     1621 tatatggcgt gaacgttgca ggtgtgctat ggggtattca agccgcacat gaacaattta
     1681 aaaaattcaa tcatggcggt aaaattatca atgcaacatc tcaagcaggc gttgagggta
     1741 acccaggctt gtctttatat tgcagtacaa aattcgcagt gcgaggttta acacaagtag
     1801 ccgcacaaga tttagcgtct gaaggtatta ctgtgaatgc attcgcacct ggtatcgttc
     1861 aaacaccaat gatggaaagt atcgcagtgg caacagccga agaagcaggt aaacctgaag
     1921 catggggttg ggaacaattt acaagtcaga ttgctttggg cagagtttct caaccagaag
     1981 atgtttcaaa tgtagtgagc ttcttagctg gtaaagactc tgattacatt actggacaaa
     2041 caattattgt agatggtggt atgagattcc gttaataatc atccactaat gataaataaa
     2101 tccttattgt taagtttaat cacttagcag taaggatttt ttagtgcact tagaagggag
     2161 tgtattggta gaaaattaat aagcgaagtt cttaagtgag ttatgatgtc acagtctaat
     2221 gcatcagttg aaagcattat tagtattaac acacccaaga tattataaaa catcacaaaa
     2281 acaccactat ctaatttatc tcaataaaaa ttcacaaagt tatctcattt tatttttata
     2341 aataaaaaat atcgataaaa agcttacaat actttatgtt tttatgatat atttttaatg
     2401 tataaatgag gtggaagatt tggaaagagt tttgataact ggtggggctg gttttattgg
     2461 gtcgcattta gtagatgatt tacaacaaga ttatgatgtt tatgttctag ataactatag
     2521 aacaggtaaa cgagaaaata ttaaaagttt ggctgacgat catgtgtttg aattagatat
     2581 tcgtgaatat gatgcagttg aacaaatcat gaagacatat caatttgatt atgttattca
     2641 tttagcagca ttagttagtg ttgctgagtc ggttgagaaa cctatcttat ctcaagaaat
     2701 aaacgtcgta gcaacattaa gattgttaga aatcattaaa aaatataata atcatataaa
     2761 acgttttatc tttgcttcgt cagcagctgt ttatggtgat cttcctgatt tgcctaaaag
     2821 tgatcaatca ttaatcttac cattatcacc atatgcaata gataaatatt acggcgaacg
     2881 gacgacatta aattattgtt cgttatataa cataccaaca gcggttgtta aattttttaa
     2941 tgtatttggg ccaagacagg atcctaagtc acaatattca ggtgtgattt caaagatgtt
     3001 cgattcattt gagcataaca agccatttac attttttggt gacggactgc aaactagaga
     3061 ttttgtatat gtatatgatg ttgttcaatc tgtacgctta attatggaac acaaagatgc
     3121 aattggacac ggttataaca ttggtacagg cacttttact aatttattag aggtttatcg
     3181 tattattggt gaattatatg gaaaatcagt cgagcatgaa tttaaagaag cacgaaaagg
     3241 agatattaag cattcttatg cagatatttc taacttaaag gcattaggat ttgttcctaa
     3301 atatacagta gaaacaggtt taaaggatta ctttaatttt gaggtagata atattgaaga
     3361 agttacagct aaagaagtgg aaatgtcgtg aaaatgacat tgaagctgtc cataataata
     3421 agggttatgc ctatcaaaga aaattagaca aactagaaga agtgagaaaa agctattacc
     3481 caattaaacg tgcgattgac ttaattttaa gcattgtttt attattttta actttaccga
     3541 ttatggttat attcgccatt gctatcgtca tagattcgcc aggaaaccct atttatagtc
     3601 aggttagagt tgggaagatg ggtaaattaa ttaaaatata caaattacgt tcgatgtgca
     3661 aaaacgcaga gaaaaacggt gcgcaatggg ctgataaaga tgatgatcgt ataacaaatg
     3721 tcgggaagtt tattcgtaaa acacgcattg atgaattacc acaactaatt aatgttgtta
     3781 aaggggaaat gagttttatt ggaccacgcc cggaacgtcc ggaatttgta gaattattta
     3841 gttcagaagt gataggtttc gagcaaagat gtcttgttac accagggtta acaggacttg
     3901 cgcaaattca aggtggatat gacttaacac cgcaacaaaa actgaaatat gacatgaaat
     3961 atatacataa aggtagttta atgatggaac tatatatatc aattagaaca ttgatggttg
     4021 ttattacagg ggaaggctca aggtagtctt aatttactta ataagttcaa ataaaagtta
     4081 tattttaaag attgtgacca attgttacag tataacgagg aatcccttga gacagtatca
     4141 aatggcatta agaaatatgt gccatcattg atttgcatgg ctataaatac tattcatctg
     4201 atgagatagc catgttaaga aattgaaagt atagcattaa aggggtttgt aacagttgaa
     4261 aattatatat tgtattacta aagcagacaa tggtggtgca caaacacatc tcattcaact
     4321 cgccaaccat ttttgcgtac acaatgatgt ttatgtcatt gtaggcaatc atggaccaat
     4381 gattgaacaa ctagatgcaa gagttaatgt aattattatc gaacatttag taggtccaat
     4441 tgactttaaa caagatattt tagctgtcaa agtgttagca cagttattct cgaaaattaa
     4501 acctgatgtt atccatttac attcttccaa agctggaacg gtcggacgaa ttgcgaagtt
     4561 catttcgaaa tcgaaagaca cacgtatagt ttttactgca catggatggg cttttacaga
     4621 gggtgttaaa ccagctaaaa aatttctata tttagttatc gaaaaattaa tgtcacttat
     4681 tacagatagc attatttgtg tttcagattt cgataaacag ttagcgttaa aatatcgatt
     4741 taatcgattg aaattaacca caatacataa tggtattgca gatgttcccg ctgttaagca
     4801 aacgctaaaa agccaatcac ataacaatat tggcgaagta gttggaatgt tgcctaataa
     4861 acaagattta cagattaatg ccccgacaaa gcatcaattt gttatgattg caagatttgc
     4921 ttatccaaaa ttgccacaaa atctaatcgc ggcaatagag atattgaaat tacataacag
     4981 taatcatgcg cattttacat ttataggcga tggacctaca ttaaatgatt gtcagcaaca
     5041 agttgtacaa gctgggttag aaaatgaagt cacatttttg ggcaatgtca ttaatgcgag
     5101 tcatttatta tcacaatacg atacgtttat tttaataagt aagcatgaag gtttgccaat
     5161 tagcattata gaagctatgg ctacaggttt gcctgttata gccagtcatg ttggcggtat
     5221 ttcagaatta gtagctgata atggtatatg tatgatgaac aaccaacccg aaactattgc
     5281 taaagtcctg gaaaaatatt taatagacag tgattacatc aaaatgagta atcaatctag
     5341 aaaacgttat ttagaatgtt ttactgagga gaaaatgatt aaagaagtgg aagacgttta
     5401 taatggaaaa tcaacacaat agtaaattac taacattgtt acttatcggt ttagcggttt
     5461 ttattcagca atcttcggtt attgccggtg tgaatgtttc tatagctgac tttatcacat
     5521 tactaatatt agtttattta ctgtttttcg ctaaccattt attaaaggca aatcattttt
     5581 tacagttttt cattattttg tatacatatc gtatgattat tacgctttgt ttgctatttt
     5641 ttgatgattt gatatttatt acggttaagg aagttcttgc atctacagtt aaatatgcat
     5701 ttgtagtcat ttatttctat ttagggatga tcatctttaa gttaggtaat agcaaaaaag
     5761 tgatcgttac ctcttatatt ataagcagtg tgactatagg tctattttgt attatagctg
     5821 gtttgaacaa gtccccttta ctaatgaaat tgttatattt tgatgaaata cgttcaaaag
     5881 gattaatgaa tgaccctaac tatttcgcga tgacacagat tattacattg gtacttgctt
     5941 acaagtatat tcataattac atattcaagg tccttgcatg tggtattttg ctatggtctt
     6001 taactacaac ggggtctaag actgcgttta tcatattaat cgtcttagcc atttatttct
     6061 ttattaaaaa gttatttagt agaaatgcgg taagtgttgt gagtatgtca gtgattatgc
     6121 tgatattact ttgttttacc ttttataata tcaactacta tttattccaa ttaagcgacc
     6181 ttgatgcctt accgtcatta gatcgaatgg cgtctatttt tgaagagggc tttgcatcat
     6241 taaatgatag tgggtctgag cgaagtgttg tatggataaa tgccatttca gtaattaaat
     6301 atacactagg ttttggtgtc ggattagtgg attatgtaca tattggctcg caaattaatg
     6361 gtattttact tgttgcccat aatacatatt tgcagatctt tgcggaatgg ggcattttat
     6421 tcggtgcatt atttatcata tttatgcttt atttactgtt tgaattattt agatttaaca
     6481 tttctgggaa aaatgtaaca gcaattgttg taatgttgac gatgctgatt tactttttaa
     6541 cagtatcatt taataactca agatatgtcg cttttatttt aggaattatc gtctttattg
     6601 ttcaatatga aaagatggaa agggatcgta atgaagagtg attcactaaa agaaaatatt
     6661 atttatcaag ggctatacca attgattaga acgatgacac cactgattac aatacccatt
     6721 atttcacgtg catttggtcc cagtggtgtg ggtattgttt cattttcttt caatatcgtg
     6781 caatactttt tgatgattgc aagtgttggc gttcagttat attttaatag agttatcgcg
     6841 aagtccgtta acgacaaacg gcaattgtca cagcagtttt gggatatctt tgtcagtaaa
     6901 ttatttttag cgttaacagt ttttgcgatg tatatggtcg taattactat atttattgat
     6961 gattactatc ttattttcct actacaagga atctatatta taggtgcagc actcgatatt
     7021 tcatggtttt atgctggaac tgaaaagttt aaaattccta gcctcagtaa tattgttgcg
     7081 tctggtattg tattaagtgt agttgttatt tttgtcaaag atcaatcaga tttatcattg
     7141 tatgtattta ctattgctat tgtgacggta ttaaaccaat tacctttgtt tatctattta
     7201 aaacgataca ttagctttgt ttcggttaat tggatacacg tctggcaatt gtttcgttcg
     7261 tcattagcat acttattacc aaatggacag ctcaacttat atactagtat ttcttgcgtt
     7321 gttcttggtt tagtaggtac ataccaacaa gttggtatct tttctaacgc atttaatatt
     7381 ttaacggtcg caatcataat gattaataca tttgatcttg taatgattcc gcgtattacc
     7441 aaaatgtcta tccagcaatc acatagttta actaaaacgt tagctaataa tatgaatatt
     7501 caattgatat taacaatacc tatggtcttt ggtttaattg caattatgcc atcattttat
     7561 ttatggttct ttggtgagga attcgcatca actgtcccat tgatgaccat tttagcgata
     7621 cttgtattaa tcattccttt aaatatgttg ataagcaggc aatatttatt aatagtgaat
     7681 aaaataagat tatataatgc gtcaattact attggtgcag tgataaacct agtattatgt
     7741 attattttga tatattttta tggaatttac ggtgctgcta ttgcgcgttt aattacagag
     7801 tttttcttgc tcatttggcg atttattgat attactaaaa tcaatgtgaa gttgaatatt
     7861 gtaagtacga ttcaatgtgt cattgctgct gttatgatgt ttattgtgct tggtgtggtc
     7921 aatcattatt tgccccctac aatgtacgct acgctgctat taattgcgat tggtatagta
     7981 gtttatcttt tattaatgat gactatgaaa aatcaatacg tatggcaaat attgaggcat
     8041 cttcgacata aaacaattta agtaccggta atgctatact ttagaaaatt aagattaaga
     8101 agaaaaggca atttcttatt gaaaaatgga agttgtcttt tttaattctc tttaaaagcg
     8161 ggaaacaaaa gcagttaaat
//
LOCUS       DAKTGS010000128         8108 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.128, whole genome shotgun sequence.
ACCESSION   DAKTGS010000128 DAKTGS010000000
VERSION     DAKTGS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.128"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            7..546
                     /locus_tag="O0286_002303"
     CDS             7..546
                     /locus_tag="O0286_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170660.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            559..1346
                     /locus_tag="O0286_002304"
                     /pseudo
     CDS             559..1346
                     /locus_tag="O0286_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            1807..2015
                     /locus_tag="O0286_002305"
                     /pseudo
     CDS             1807..2015
                     /locus_tag="O0286_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2589..2677)
                     /locus_tag="O0286_002306"
     tRNA            complement(2589..2677)
                     /locus_tag="O0286_002306"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2640..2642),aa:Ser,seq:gga)
     gene            complement(2732..2803)
                     /locus_tag="O0286_002307"
     tRNA            complement(2732..2803)
                     /locus_tag="O0286_002307"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2768..2770),aa:Glu,seq:ttc)
     gene            complement(2805..2879)
                     /locus_tag="O0286_002308"
     tRNA            complement(2805..2879)
                     /locus_tag="O0286_002308"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2845..2847),aa:Asn,seq:gtt)
     gene            complement(2894..2967)
                     /locus_tag="O0286_002309"
     tRNA            complement(2894..2967)
                     /locus_tag="O0286_002309"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2933..2935),aa:Gly,seq:tcc)
     gene            complement(2983..3058)
                     /locus_tag="O0286_002310"
     tRNA            complement(2983..3058)
                     /locus_tag="O0286_002310"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3023..3025),aa:His,seq:gtg)
     gene            complement(3072..3144)
                     /locus_tag="O0286_002311"
     tRNA            complement(3072..3144)
                     /locus_tag="O0286_002311"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3109..3111),aa:Phe,seq:gaa)
     gene            complement(3161..3236)
                     /locus_tag="O0286_002312"
     tRNA            complement(3161..3236)
                     /locus_tag="O0286_002312"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3201..3203),aa:Asp,seq:gtc)
     gene            complement(3258..3331)
                     /locus_tag="O0286_002313"
     tRNA            complement(3258..3331)
                     /locus_tag="O0286_002313"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3295..3297),aa:Met,seq:cat)
     gene            complement(3803..4357)
                     /locus_tag="O0286_002314"
     CDS             complement(3803..4357)
                     /locus_tag="O0286_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCY0170661.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(4680..6080)
                     /locus_tag="O0286_002315"
     CDS             complement(4680..6080)
                     /locus_tag="O0286_002315"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCY0170662.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(6104..7027)
                     /gene="hemH"
                     /locus_tag="O0286_002316"
     CDS             complement(6104..7027)
                     /gene="hemH"
                     /locus_tag="O0286_002316"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCY0170663.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(7085..>8108)
                     /gene="hemE"
                     /locus_tag="O0286_002317"
     CDS             complement(7085..>8108)
                     /gene="hemE"
                     /locus_tag="O0286_002317"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCY0170664.1"
                     /translation="NNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYSLFDIT
                     HQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPIKTIQD
                     VEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSKNYNFT
                     KAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYRRYIKP
                     HMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQLGVTKT
                     LQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRKVSEFV
                     HTYTQR"
BASE COUNT         2717 a         1484 c         1185 g         2722 t
ORIGIN      
        1 acacttttgg ttgcgtatgc atctcaccta tcttttttgc ttttttcaaa taagaaagta
       61 ttaaaaatta atgcctatac aggaacagac atggctaaac ttgatttaaa tagtcttgac
      121 gacgagcacg taaaattatt aataaatgaa ttaaaatatc cagaaactca tatcgatgta
      181 aatgaattaa aaacaatagt tgctagtcga ataaatgaaa ggcaagaaat aataagtttt
      241 aagttaggaa taaagtactt attaacaata aaaagaggga acatagaaaa agataggttt
      301 tcaatttcaa tcattttcaa agatacctat cacaccctag ttagaataga tattaacggt
      361 ggtactcacg ataatccaga tggaacaatc gctccgaaaa gtcatattca catatataat
      421 gataagtatg ataaaaagga taggtttgct tatgaaatta acttgaaaga tttccccgac
      481 atctataact tgtataatgt gtatatgtcg tttttagagt ataataatat aaaagacctt
      541 gaataaagga gggataatat gactacattt gatgctaaaa aattaaaaaa agaatatctt
      601 gattggtata atcagacctt agagttttct aatttatcaa acaatgtagt aagaatagat
      661 actcctttta aagataattc tttagataat ttaataattt acgctttata cgatcagtcc
      721 agagacatga ttacactgac agatgacggc tatactatat ttgatttaga aaataatggt
      781 atttctttaa ataaatcaaa aaaacgtaaa aagatttttg aagagcacct ttcagcttac
      841 ggtattaaat ataacgataa aactcacgaa atttttgttc aaactaactt taaaaatttt
      901 aataaatcga aacataattt attacagtgc cttatatttg ttaatgatat gtacttactt
      961 tctaatccta agtcacagaa catatttaca gaagatgttg caaacaaatt ggatgaacat
     1021 aacatttatt acggaagaga tttacctatt ataggaagca gtggtgttgt tcataatttc
     1081 gactttttta ttagcgctaa gaaaaatcaa aaagaaaaat ttatcaatgc tatttctaac
     1141 cctaataatt ctatgattat taagtcgaaa ataacggatg ctatgcaagc aaaaaaataa
     1201 aaagacacag gcaaaatgag tttattttta ttttaaatga ctcaaaaaaa gaaataaatg
     1261 aacataataa aaatcttctt catgaaaact atattagtac aatagattat agcgaattag
     1321 atgaaaagat aggtttattg atttaatata tatagacgtg ataatatcaa tgtttatatt
     1381 aaatgaaacg aatttgaaaa tttcgaaaca gcttaagata gcaaattgaa tagccttatt
     1441 gataatgcag aatcgtctac acttagttga actaattcta tgagaataga tattgttaaa
     1501 ctatttgggt aggcgatttt attttgacaa gagtcagaag attatttagt tcatagttta
     1561 agttacaaat ggctaaactt tatgaaaatg gtatgactag aatcgaaatt atactcgaat
     1621 acgatttaac actctcaatc ttctcgaact gaataaatac tgggtcattc aatcatcaag
     1681 acaacttaat aagtgatgat aaatgttcat accattttgc aaatataacc agatactctc
     1741 aacaaaatgt agagttcaaa attaaaaatg tccctaattg gcacattgct gtatgataat
     1801 aaattcaact tagatttcaa caatataact aatgatgctt atatttaccg cgatatcaaa
     1861 ccaaacagcc catccacaaa caaattagaa tatatcatgt taataatagt aaatgatata
     1921 tcttccaaag accgcaaact gtagcagaac atgagaacct tgcgattttg aaatggtcta
     1981 ataaatcaaa tatcattgaa actttaatca cataaattaa cagtagtcac ctgttcgcag
     2041 ttctataaat tacaaatgca ataaaggcta aagtaaacta attactttag cctttattaa
     2101 taatttagtc gaatttatta aaaatcgact aactcctggt tatgctacgc tttactttat
     2161 aatccgcacg tagatgccga ttgattatat tataatatta ttattttatt ttttcaagcg
     2221 tatatataat atttgatttt ctttaagtaa cagctaaaca ttacatttca aaacgattag
     2281 gtcatgcaga tgtacataca acgcttaaaa catattcaca tttgattaaa gaatttgaag
     2341 ataatgaaac taaatttata gaaaaaaatc tgaatgattt gttttgtgac taaacttcaa
     2401 aatcttgtga ctaaaattag gattattcat caaaaattag tcacattgtg gctaaaaaat
     2461 tttaaatgga tataaataaa taagaagtgt aaaaatgagg ataatagctc aaatgcagat
     2521 atatcaaggg attgagtcca aaataaaaat agcgcttaaa atgggtaaaa ccaatttaag
     2581 cgcctttacg gaaggtaagg gattcgaacc cttgagacgc ttgtgacgcc tacacacttt
     2641 ccaggcgtgc tccttcggcc agctcggaca accttcctca acaataaata tgtaataaaa
     2701 aaacagaagc gatatttcac ttctgcttgt atgactccta cgggactcga acccgtgtta
     2761 ccgccgtgaa agggcggtgt cttaaccgct tgaccaagga gccatggctc cacaggtagg
     2821 actcgaacct acgaccgatc ggttaacagc cgatagctct accactgagc tactgtggaa
     2881 taatagaaaa taatggagcg ggtgatggga atcgaaccca caacatcagc ttggaaggct
     2941 gaggttttgc cattaaacta cacccgctaa tgtaaacgat tatggggcgg ctgaagggga
     3001 tcgaaccctc gaatgtcgga accacaatcc gatgtgttaa ccacttcacc acaaccgcca
     3061 tgaattaaga atggttcagg acagagtcga actgccgaca catggagctt caatccattg
     3121 ctctaccaac tgagctactg aaccgtaatg gtaataataa tggcggtctc gacgggaatc
     3181 gaacccgcga tctcctgcgt gacaggcagg cgtgttaacc gctacactac gagacctatt
     3241 aaattaaaaa ctatgtattg cgggaggcgg atttgaacca ccgaccttcg ggttatgagc
     3301 ccgacgagct accgaactgc tctatcccgc gctaatatta ttttgaatta cctaattaat
     3361 ataccataat caaaaaccta aagtcaagaa ctttttgaat ttaatttaaa tgttatctct
     3421 taaataatta cttaaatatc gtagcaacat gttctctgtt gaacacaaat attagtatat
     3481 tcatttttgt agtgtacgtc aacgacattt tcaaagtttt ttgtgtaaaa aaacgcttct
     3541 tattcccttt tatcatataa gtgtctaata gttgtcataa atagtgttaa agcatttaaa
     3601 aaggtatagg agttataaag tttacaacgc ctataccttc tgaaaaagaa attataaaag
     3661 cttgttacac cgcatattct ttcagtcagc gactaccaat ataacattgt agccctaaga
     3721 cattgcttga cgcctcaatt acaacaattt ttcaaaatca gcagctacct actgacacaa
     3781 cataacacaa cccatacact aactatcgtg tcatgtaatc ttgcatccga tcttgcaacg
     3841 ctgtaaatgt ttcgaagcca tcctcttcta agaagtgccc tccatcttcc acgattcgca
     3901 agttcccctc taatgcattc attaaacgct gggtctcttt atatgaaacg tatttgtcat
     3961 ttttagaact caatccgtaa aaattgtcaa ctttcttttt aatattatcg taatcaatgg
     4021 ttacattact taaatcaata tctaaatcta tattttctgc atcttcttta aagcccgcta
     4081 tactgaaaaa gccttcaatc ggctgatcaa tcatttcaat atattttaaa gctgtgattg
     4141 aacctaaacc atgtgttaca aaatatgtat cctttttgcg tacattaatt tgtttcgtca
     4201 tagcttcaat ccactgatcc actgtcttcg cttcagggga ttcaaaatta aataatgtta
     4261 cgtcatatcc ttctaaagtt aagttatgct ccaaccactg ataccaatga tttctactat
     4321 ttccatgcat agaatgtaca ataattacat ctgtcatctc attctctcct ttcaacttac
     4381 tacttctttt ctatttttaa aaaaatgact gattacctat aattgtaaaa taaaaacacc
     4441 ttaattagaa atgttatatc gcaaagtgac atttctaatt aaagtgtatt gtcatcattt
     4501 caatatcatt caaaaacagc taaacctttg tctctgcttc aatttcacaa aaataattcc
     4561 cgctgaaagt atctatattt acacattact tccaccatta tataacttaa aaatgactat
     4621 atttcatcaa acattatcta aaggcgtcgc acctacacca acaccatcca acaattaact
     4681 tacaactctg cgattacttc ttcagcagca actttacctt gcgtaataca atcaggtagt
     4741 ccaaccgctt caaaagatgc accagttact ctaagtcgtg gatatgtttg tttaatatgt
     4801 gcttgaatct gtctaatttg ttgaatatga ccgacatggt actgtggcat acttttcggc
     4861 aaacgattga caattgtaaa ttcaggatca cctttaaatg tcatcatttg acttaaatct
     4921 ctacgtacaa tcgatactaa ttcattatct gtatgatcat caaccacagt atcacctggt
     4981 ttacctacat acgcacgaat caaaacctta ccttccggtg tagtaaatgg ccattttttc
     5041 gatgtccaag tacatgcggt aatgtctgta tcactcgttc tcgcaattac gaagccagta
     5101 ccatcatggg tattttcaat gtctttttca tcaaatgcca atacaacagt tgcaacagtc
     5161 gtactatcca tcgttttaaa gtaatcaaat gctggatctt gtccgaacca atttaaaaac
     5221 acttgatgtg gtgtcgttac taatacgcca tcatacactt cttctagttg atcattgtaa
     5281 acaattttat attgtttttg agatgtaatt atatcatcca ctgacgtatt gtagcgtatt
     5341 gtcacacctt tatttttaac atcttgttct aatgcttcaa taaatgagct taaaccatgc
     5401 ttaaattgtt tgaattgtcc tttcggtgcg ccaggatata attgtctttg tttcagacgc
     5461 ttatttttct catccttcat accttttatc agacttccga atgcctcttc tttttcttta
     5521 aaattaggaa acgtactcat caaacttaat ttatcaatat cggtaccata aataccaccc
     5581 attaaaggct ctattaaatt ctcaagtacc tcattaccta atcttgctct gaaaaatgca
     5641 ccaacagaaa tgtcaccatc ctgcatttga gtaggttttt ttaataaatc aaaccctgct
     5701 cttaatttac caagtggcga tattaatttt gtagtaacaa atggtttaat atctgttgga
     5761 atacccataa ttgaaccacc tggaatcgga tataatttat ttttcgcaaa aatatatgat
     5821 tgtccagtcg tatttgtaac aatatcttgt tctaatccaa tatctttcgc taattctgtc
     5881 ataatcgttt ttctacctaa ataagattca ggccctagtt caatcatata accatcttta
     5941 cgatacgatt gaatctttcc ccccggacga ttcgatgctt caaagatggt tacatcaata
     6001 ttaggatctt gctgttttaa aaaatatgca cttgataaac ctgttatccc cgctcctata
     6061 atagccactg atttagtcac aacgtttcac gcttctttcg tcattaaaat atagacttga
     6121 tttcatcaac aattgcaccg ataaataatg gatgtgtatt cggcattttt ggacgataat
     6181 aattcgcacc aatatcatcg caaactactt tacattcata atcattgtca taaagcacct
     6241 ctaaatgctc acatacaaaa cctactggcg tatatataaa gtttttatac tgatgttttt
     6301 catataaatc acgtgttaaa tcttgtacat ctggccctaa ccaaggtgta cctgtattac
     6361 cttcagattg ccaaccaatc gcgatatgtt caatattaga ttgttcttta attaaaagcg
     6421 cagtatgttc tagttcttgt ggatatggat cattattctt ttcgattaaa ccttttggca
     6481 aactatgtgc cgaaacaact aataccgtgt ctttatgttc ctcttccggt atttgagcta
     6541 atgtttcgtt gactttattc gtccaatatt caataaattt aggttgttca taataatgtt
     6601 tcacatgtgt aagttgaata ccatattttg cagcttcttc atcagcacgt ttgtcatatg
     6661 atcctactga aaatgaagaa taatgtggtg ctagtactac cgtgattgct tcagtaatgc
     6721 catcattgtg catttgttca accgcatctt cgataaatgg tgaaatgtgt tttaatccta
     6781 agtatagttt aaattcaaca tctgcatatg ctttatttaa tgctgaaact agcgcatcag
     6841 cctggtcatc tgttgtacct gctaatggtg ataaaccacc tataaattca tatctatctt
     6901 tcaaatcttg aagttcttct tcagatggac gtttaccatg tctaatatct gtataatatg
     6961 gctctatgtc actttcttta taaggtgtgc cataagccat aactaataat cccatttttt
     7021 tagtcattga taataccttc ctttaaatga attatctttc atgtgcttca atgtaaaact
     7081 atgattatct ttgtgtatat gtgtgtacga attcgcttac tttacgtaac gtctctggtt
     7141 gcacttctgg gaatacaccg tgtcctaaat taaagatgtg tttaccgttc tccatacctt
     7201 gatctaatat tggtttcaat ctctcttcaa tgacattcca tggtgctaat aaaattgatg
     7261 gatctaaatt cccttgtaat gttttagtaa cgcctaattg ttgagcctga ttaatagacg
     7321 ttctccaatc taggcctaat acatcaatcg gtaaatcatt ccattcattg attaaatgac
     7381 tggcacctac accgaataaa attaccggca catcatgttt ttctttaacc tcactgatta
     7441 atcgaatcat atgtggttta atgtaacgtc tgtaatcctc gacatttaat gcacctaccc
     7501 atgaatcgaa aatttgaatc aattcggcac ctgcttcgac ttgagctgtt acatatttaa
     7561 cagatacatc aactaaatga ttcattaaag caaaccatgt tgcttcatct ctatacatca
     7621 tcgcttttgt aaaattgtaa tttttcgatg gtccgccttc aatcatatat gacgctaatg
     7681 taaatggtgc cccagtaaat cctattagcg gcacatttaa cttttcttct gttaaaagtt
     7741 taattgtatc taatacatat ggtacatctc gttcggggtc tatttgagaa agtttctcaa
     7801 catcttgaat tgttttgatt ggattatgaa tcactggacc aatacccgat ttaatttcta
     7861 catcgacacc aattggcttt aatggtgtca taatatcttt gtataaaatt gctgcatctg
     7921 tatgataatt atcaactggt aaatgtgtta cataagcgca caactccggc tgatgtgtaa
     7981 tatcgaatag tgaatatttt tctttcaatt ttcgatattc tggttgcgaa cggccagctt
     8041 gtcgcataaa ccaaacaggt gtatgtgatg tttcttcacc tttgatcatt tttaaaattg
     8101 tattgttt
//
LOCUS       DAKTGS010000129         7877 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.129, whole genome shotgun sequence.
ACCESSION   DAKTGS010000129 DAKTGS010000000
VERSION     DAKTGS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.129"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            231..377
                     /locus_tag="O0286_002318"
     CDS             231..377
                     /locus_tag="O0286_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170665.1"
                     /translation="MKLFYIVFLIIIWLNIFLGNEIIHTLTVLITTLYIVNSRKGIKN
                     DRVE"
     gene            334..558
                     /locus_tag="O0286_002319"
     CDS             334..558
                     /locus_tag="O0286_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170666.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
     gene            complement(1003..1719)
                     /locus_tag="O0286_002320"
     CDS             complement(1003..1719)
                     /locus_tag="O0286_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCY0170667.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(1827..2552)
                     /locus_tag="O0286_002321"
     CDS             complement(1827..2552)
                     /locus_tag="O0286_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCY0170668.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            complement(2647..3372)
                     /locus_tag="O0286_002322"
     CDS             complement(2647..3372)
                     /locus_tag="O0286_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCY0170669.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            3810..4811
                     /gene="argF"
                     /locus_tag="O0286_002323"
     CDS             3810..4811
                     /gene="argF"
                     /locus_tag="O0286_002323"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0170670.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            4834..5766
                     /gene="arcC"
                     /locus_tag="O0286_002324"
     CDS             4834..5766
                     /gene="arcC"
                     /locus_tag="O0286_002324"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0170671.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            5938..7494
                     /locus_tag="O0286_002325"
     CDS             5938..7494
                     /locus_tag="O0286_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCY0170672.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
BASE COUNT         2665 a         1079 c         1371 g         2762 t
ORIGIN      
        1 attaattatg tttggaattt cgtcgggaat aaattttgcc attacataga aatatctaat
       61 agataacgaa aaagtatcgt atgtattttt aatatagtgt aaaatattat atgtaaaata
      121 aaatgtaggt ttttagttag aggcattata agaaaatttt gagtataggt tagcttttaa
      181 ttatgaatct tattgaaatt tgattaataa aaatatgata ggggattaaa atgaaactat
      241 tttatatcgt atttcttatt attatatggc tgaatatatt tttaggaaat gaaatcatcc
      301 atacactgac tgttttaata acaacattgt atattgttaa ttcaagaaag gggattaaaa
      361 atgacagagt tgaataatat tataaactct cttcaatctt tgtttgagtc tgaatcaggc
      421 tataaaattt cgaaaaattc aggagttcca tatcaaacag tacaagattt aagaaatggg
      481 aaaaccaaac tagaagatgc tagatttaga acgattatta aactttatag ttactatgtc
      541 tcattaaaag aacactaatc atgggacaat aaagtattgc tataataatt taatcattaa
      601 atgaaggaat agagcctaag acataataaa tcaatgtctt aggctctaca atattatatt
      661 ggtagtagtt gaccgaatga aaatgcccat gtaacaagca tttttcaatt ataggcgggg
      721 ccccaacata gagaatttca aaaagaaatt ctacagacaa tgcaagttgg ggtgggcccc
      781 aacacagaag ctgacgaaaa gtcagcttac aataatgtgc aagttgggga gtgacaacga
      841 aataaatttt ataaaatact atttctgtca cattcctact cccaattcca tttaaatata
      901 tagaaaattt tacctattat atacatcata cactttaaaa attatcattt aaataatctg
      961 atttggaatt aaagtgcatg aagtataagt caccttcata tactaatcaa agaggacgtc
     1021 aacagttatt ttattaggat ttttaacata aacatttgct agatctgaat gtaatctttt
     1081 gcttaaatca atagtgtagt tattaccgcc accggtgatc ttaagcttac ctttattacg
     1141 attttcgtta tataatattt tattttttat taacgcttct cgtgcacgga aatcgatttc
     1201 tttcaatgtt aatactggtt tattgccttg gtatatttta tgcgcaccaa taatcgtttg
     1261 tagtttatct ttgtattgca caaaaagatt ataagtttta tcagaaggtt ttgcggctgg
     1321 tgtaacgcca cctgtaaatg tctctctata agaccaccat aactgatcag tatctttgtc
     1381 ttttagtcca aacacatcta cgtaacgatc ttttaactga ttaatatttc cccaactttc
     1441 agcgccccat aaagatatat gtgctgacca tgaatatctc ttaagttcaa cataaatgtt
     1501 tccattatca tattgatata gccatttatt tgaaaatgaa aaatgaggct gtgtgtaata
     1561 tttaattaat tcattgatgt tagtttcatc ttgaccaatg ctataagctt tagcttcaga
     1621 gtaaaaacta aatactggtt tttgattttg aggtaataca gtacctaata gtaataatgt
     1681 tgtcgttaaa attatatttt tcgtgatgtt cttactcatt agaacatctc ctttcagagg
     1741 aatcatgata cgaggaataa gaaatttaaa tgtgagcgaa gtcaatatag tatttgcgat
     1801 tatttttatt aactccatta ttattgttag tttgattttt cgaggataac ttcaattttt
     1861 gcattttgag gttttttaac atatctattt gcatcagttg atggcaacct tttacttaaa
     1921 tctattgtgt agttattgtc tgcacctgtt attttaattt gtcctttatt ataagaatta
     1981 ttatataatt ttttactttt aattaatgtt tgacgaatac gaaaatctaa ttcttttaaa
     2041 gttaaaacag gcttattgcc ttcataaact ggaaatccgc cagtaaacgt ttctgcttta
     2101 tctttatatg ttacattcag tttatagtgt ttatcgttag atgttgctgc aggagtaaca
     2161 ccaccagtaa acgtttcttg agataatgca aaagaatcaa tggtttcttg gtcttttatg
     2221 ccaaaaatat caacgctttt atttcttaat tggttgatat tgccccaact ttcaggtcca
     2281 taaacttgaa tatgactata ccaagaaaac tgtaacaacg ttgcatgaat cgtaccgtta
     2341 tctttttgcc ataacgtact gttagagaag gttaaatatt tttgcgagta atatttagtt
     2401 aactcattaa cgttagtttc gttttgattt atataataag ctttcgcttc agatgaagaa
     2461 ttgataggtg tattaggaaa ttgtgtagat gctgtaccta atagtaacaa tgttgttgat
     2521 aaaataattt ttttcgtgat gttattgttc attcgaattt ctcctttgag tattgttgga
     2581 atgtttaatt ataaaaaggg gttaattaga taattgaaat tatccgcatt tacaaaaggt
     2641 aataggttag ttagattttt cgagtatgac ttcaatttct gcattacgag gatttttaac
     2701 ataacggttt gtgtcagtta attttaactt tttacttaaa tcaatcgtgt aattatttcc
     2761 atcagcagtt atcttaattt gacctttatt aaattctccg ttatataact ttttattttt
     2821 tattaatgtt tgacgaatac ggaaatctaa ttcttttaaa gttaatactg gtttatttcc
     2881 tttgtaaaat tcatgtccac cgatgatagt ttgttgttta tcactatatt ttaaaaatag
     2941 tctataaggc ttatcagatg aagtagctgc tggcgtaaca ccaccagtaa atgtttcatc
     3001 ataagtccag taaccttcaa ctgtgtcctc atctttagtt ccaaatatat caacgtattt
     3061 atttcttaac tgattaatgt ttccccaact ctcggatcca aacacttgaa tatgactata
     3121 ccaaacccac gtttgcaatg ttgcatgaat gctaccattg ggcttttgcc ataaccattt
     3181 tccagataat gaaaaatgcg gctgagtgta atatttgatt agttcattga tattagtctc
     3241 gttttcactg atattatagg cttttgcttc agatgaaaaa ctgattggtg ttttaggaag
     3301 ttgtgttgat gtagtttcta aaagtaacaa tgctgttgat aaaactaatt tattcatgat
     3361 gttctttttc atatgaaaat ctcctttgcg tgaattaccc aaagtatata agctattaca
     3421 ccgattcgga attaaataaa agctaaaact atgttaaata aacttaaaca gttagtagtg
     3481 ttatttaagc aaaacttatc atttttaagt tttggacaga aacagtatct aataaagtag
     3541 gcgggagtta tatattaaaa acgatacgtt agaattattc tctaatcaat tgtattaaat
     3601 tgtttgataa ttgaattttc taactacttg aaaaatagtt atactttaaa tgtagtactt
     3661 attttaatta tttcctacta cttaaattta atattaataa aatgttcatt taattattga
     3721 taaaatatta caaattttaa tagtaggttg tgtttatttt gtatgcgctt acaatttagg
     3781 tgtaactaaa ataaaaggag ttgttattaa tgaaaaattt acgaaacaga agttttttaa
     3841 ctttattaga cttttcacga caagaggtag aattcttatt aacactctcc gaggatttaa
     3901 aacgtgctaa atatattggc actgaaaagc ctatgttaaa aaataaaaat attgcactgt
     3961 tatttgaaaa agattctaca agaacgcgat gtgcatttga agttgcagcg catgatcaag
     4021 gtgcaaatgt aacttattta ggcccaactg gatcacaaat gggtaaaaaa gaaacaacta
     4081 aagatactgc acgtgtgctt ggtggaatgt atgatggcat tgaataccgt ggtttttcac
     4141 aaagaacagt agaaacttta gctgagtatt caggcgtacc agtgtggaat ggtttaactg
     4201 atgaagatca tcctactcaa gttcttgctg atttcttaac agcaaaagaa gtcttaaaaa
     4261 aagattatgc agatattaac tttacatatg ttggagatgg tcgtaataac gttgcaaatg
     4321 cattaatgca aggtgctgcc attatgggta tgaacttcca tttagtttgt ccaaaagaat
     4381 taaatccaac agatgaatta ttaaatcgct gtaaaaatat tgccgctgaa aatggtggca
     4441 acatattaat cacagatgat attgaccaag gtgtaaaagg ttcggatgta atttacactg
     4501 atgtttgggt atcaatgggt gaacctgatg aagtatggaa agaacgactt gaattattga
     4561 aaccatatca agtaaataaa gaaataatgg ataaaactgg taatccaaat gttatttttg
     4621 agcattgctt accatctttc cataatgctg atacgaaaat aggtcaacaa atttttgaaa
     4681 aatatggtat tcgagaaatg gaagttacag atgaagtatt cgaaagtaaa gcttcagttg
     4741 tattccaaga agctgagaac agaatgcata caatcaaagc agtcatggtt gctacattgg
     4801 gtgaatttta aatgatataa ggaagtgaat atgatggcga aaatagtagt agcattaggt
     4861 ggtaatgctt taggaaaatc acctcaagaa caactcgagc ttgttaaaaa tactgcgaaa
     4921 tcattagtag gattaataac aaaaggacat gagattgtta ttagtcatgg taatggacca
     4981 caggttggaa gcattaattt gggacttaac tatgctgcag aacataacca aggtccggca
     5041 tttccatttg ctgaatgtgg cgcaatgagt caagcttaca tcggctatca attacaagaa
     5101 agcttacaaa atgaattgca ttctattgga atggataaac aagtggtaac actagtgaca
     5161 caagttgaag ttgatgaaaa tgatccggca tttaacaatc cttcaaaacc aattgggtta
     5221 ttttacaaca aagaagaagc tgaacaaatt caaaaagaaa aaggatttat atttgttgaa
     5281 gatgctggaa gaggatatag acgcgttgtt ccttcaccac aacccatctc tattattgaa
     5341 ttagagagta ttaaaacact tattaaaaat gatacactcg ttattgctgc tggtggtgga
     5401 ggtataccag taattagaga gcaacatgat ggttttaaag gtattgatgc agttatagac
     5461 aaagataaaa caagtgcgtt gttgggtgct aatattcaat gcgatcaatt gattatttta
     5521 acagcaattg attatgtata tattaatttt aacactgaaa accaacagcc tttgaaaaca
     5581 acaaatgttg atgaattaaa acgatatata gacgaaaatc aatttgcaaa aggaagtatg
     5641 ttaccaaaaa ttgaagcagc catatcattt attgaaaaca atccaaaagg aagtgtgctt
     5701 ataacatcat taaatgaatt agatgctgcc ttagagggta aagtaggtac tgtgattaaa
     5761 aagtaattga attgaaacgc ttttcaatta ctatatgtca aatgcatgat ttttattatt
     5821 tatgtgcacc ccctgaaaat aatgcctcta ttttgatgcg gggtgcactt tcttaattta
     5881 tatttataaa atctttaagg tagaaatgct aggttaaatg tcgaaggaga tgaaaccgtg
     5941 gaaaatacaa ttaatgaaag tgaaaagaaa aaacgattta aattaaaaat gccaggtgca
     6001 tttatgattt tattcatttt aacggttgtt gcagttatag caacatgggt tattcctgct
     6061 ggtgcatatt ctaaactttc ttacgaacct tcatcccaag aactaaagat agttaaccct
     6121 cataaccaag tgaaaaaggt tccgggtacg caacaggaac tagacaaaat gggggttaaa
     6181 attaagattg aacaatttaa atcaggtgca attaataagc cggtatcaat tccgaatact
     6241 tatgaaagat taaagcaaca tccagctgga ccagaacaaa taacaagtag catggttgaa
     6301 ggtacgatag aagcggtcga tatcatggta ttcattcttg tactaggggg acttattggc
     6361 gtagttcaag ccagtggttc ttttgaatcg ggattgttag ctttaacgaa gaaaacaaaa
     6421 gggcatgaat ttatgctaat tgtgtttgta tcaatactaa tgattatcgg cgggacatta
     6481 tgtggtattg aagaagaagc tgtagcattt tatccgattt tagtccctat atttatagcg
     6541 ttaggatacg attctatcgt ttcagttggt gccatattcc ttgccagctc tgtcggtagt
     6601 acattttcaa ctattaaccc gttctcggtt gtaattgcct ctaatgccgc tggtacaact
     6661 tttacggatg gcttgtattg gagaataggt gcttgtattg tcggtgcgat ttttgttatt
     6721 agttatttat attggtactg taaaaaaatt aaaaacgatc ctaaagcgtc atattcttat
     6781 gaagacaaag atgcttttga acagcaatgg tctgtattaa aagatgacga tagtgcccat
     6841 tttactttgc gtaagaagat aatccttaca ttatttgtac taccatttcc aattatggta
     6901 tggggagtta tgacgcaagg ttggtggttc ccagttatgg cttcagcatt tttaatattt
     6961 acaattataa taatgtttat tgctgggaca ggtaaatctg gattgggaga aaaaggaact
     7021 gtagatgcat ttgtcaatgg tgcatcaagt ttagtaggtg tatctttaat tattggttta
     7081 gctcgaggta ttaatttagt gttgaatgaa ggtatgattt cagatacaat cttacacttt
     7141 tcatcatctt tagttcaaca tatgagtgga ccattattta tcatcgtatt actatttatt
     7201 ttcttctgtt taggttttat cgtgccatca tcttctggct tagcggtatt atcaatgcct
     7261 atctttgcac cactagctga tacagtaggt ataccaagat tcgtcatcgt tacgacatat
     7321 caatttggtc aatatgcgat gttattctta gcgccgactg gacttgttat ggccacacta
     7381 caaatgttaa acatgcgata ttcacattgg ttccgatttg tatggccggt agttgctttt
     7441 gtattgattt tcggtggcgg agtactaatt acgcaagtac taatttattc ataatttgaa
     7501 atgctatatt ataaaaaata ctaatggggt tttatgcatc tcgtaggttt gtagaaatac
     7561 taaactaagc gaggtgcatt attatttttg attaagaaaa taatgacggt aatgataaca
     7621 ctagtaagta attgatacaa tgctctattt aataatgata tttttaaaat ttgtttttaa
     7681 tgtaatgtta gatctatggt atattatatt tatcgtggta aatatgtatt tgctgtaaac
     7741 agtttttgta acacatgata catatggttg tgaaaatagc atatataaag gatggctata
     7801 aatgacacat ttgacaaagg ttttagatac actaactgga atatgcgtag tattattatt
     7861 tagtaaatat tttgtgg
//
LOCUS       DAKTGS010000130         7810 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.130, whole genome shotgun sequence.
ACCESSION   DAKTGS010000130 DAKTGS010000000
VERSION     DAKTGS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.130"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..852
                     /locus_tag="O0286_002326"
     CDS             <1..852
                     /locus_tag="O0286_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0170673.1"
                     /translation="INKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRLSRAKLYT
                     IATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDDTDVEIDN
                     LTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLMEAINIMF
                     GSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDFKNVLGDV
                     SARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGSTLASIISG
                     SIIGLVL"
     gene            complement(957..2489)
                     /locus_tag="O0286_002327"
     CDS             complement(957..2489)
                     /locus_tag="O0286_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCY0170674.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(2529..3410)
                     /locus_tag="O0286_002328"
     CDS             complement(2529..3410)
                     /locus_tag="O0286_002328"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCY0170675.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            3568..4428
                     /locus_tag="O0286_002329"
     CDS             3568..4428
                     /locus_tag="O0286_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCY0170676.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(4706..5506)
                     /locus_tag="O0286_002330"
     CDS             complement(4706..5506)
                     /locus_tag="O0286_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0170677.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            5646..6314
                     /locus_tag="O0286_002331"
     CDS             5646..6314
                     /locus_tag="O0286_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCY0170678.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(6441..7754)
                     /locus_tag="O0286_002332"
     CDS             complement(6441..7754)
                     /locus_tag="O0286_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0170679.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         2657 a         1304 c         1457 g         2392 t
ORIGIN      
        1 attaataaaa taactagaat ggggcgctta gaaagttatt ttgctatttc aacagcaatg
       61 tttgggcaac cagaagtata tttaacaata aaagatatta ttccaagatt atctagagcg
      121 aaattatata caattgcgac gtctggtatg agtgctgtta gtatggcaat gctaggttca
      181 tatatgcaga tgattgaacc caagttcgta gttacagcag taatgttaaa tatttttagt
      241 gcgcttatca tcgccagtgt aatcaatccc tataaatctg atgatactga tgttgaaatt
      301 gataacttaa cgaaatccac agaaactaaa acattgaatg gaaaaacagg aaaacctaag
      361 aaagttgcct ttttccaaat gattggtgat agtgcgatgg atgggtttaa aatcgctgtt
      421 gtagtagccg taatgttgtt agcatttatt tcattaatgg aagcaattaa tatcatgttt
      481 ggtagtgttg gtttgaactt taaacagctt attggctatg tgtttgcacc aatcgcattc
      541 ttaatgggga ttccatggag cgaagctgtt ccagctggct ctttaatggc gactaaatta
      601 attacaaatg agtttgtagc aatgcttgat tttaaaaatg tcctgggtga tgtatcagct
      661 cgaacacaag gtatcatttc agtttactta gtaagcttcg ctaattttgg tacggttggt
      721 atcatcgtag gttcaattaa aggcattagt gataaacaag gagaaaaagt tgcatccttt
      781 gcaatgaggt tgctacttgg ttcaactcta gcttcaatca tttcaggatc aatcattggc
      841 ttagtattgt aaatgaatcg aagtacctaa attaaattca tggcaaagct aaaccccgtc
      901 accaagttgg cgcaacagcg catcataact tagtgacggg gttttatcat aacaatctac
      961 tttttcgtag ccgtttttga aatgtatgtt gatggtttat ctttttcaaa aattgttaat
     1021 cccgttatat cttttttatg ttttgaaggg acaatgaagc taagtatata agcaaagaca
     1081 aaagcaactg taaatgaaat ggtagataca tagaaaggtg agttaccttt gccaacacca
     1141 ttatagacat aagcaaagat gatacccaat attaatccac aaataacacc gaatgtattc
     1201 gtacgtttag tgaaaatacc aactgcaaat acaccagcca atggaacgcc gaataatcca
     1261 gtcacaaaca agaataaatc ccataagtca tttgaattag aagcaattaa gtatagtgac
     1321 attccaaaac cgaaaatacc tgcaatgata ataatgaaac gtgcaaagtt aacttcgtgt
     1381 cgctcgctac cttttccgaa gaagcgttgc ttaatgtcga ttgaaataca agcagatata
     1441 gaatttaaac tagatgaaat ggtagactgt gcagcggcga aaatggctgc aataagtaat
     1501 cctgctacaa atggtggcat ctcagtcaaa atgaaatatg gcactacaga tgatgtattg
     1561 aagccttttg gtaaaacagc ttcatgtgta taaaatgaat acagcattgt acccatacca
     1621 taaaataagg gtgctgaaat taaagctagg ataccatttg tccataacga tttatttgtt
     1681 tcttttaaac tatcagaagc ttgataacgc tgcacgacgt cttgactcgc tgtgtattga
     1741 tacaagttgt tgaaaatatt tcctaggaaa ataattggaa tggcagctgc cgcagtattt
     1801 agtttccaat tgtctgcact aattaatttt ttgtgctcaa tcgcatctgc aaagacagtg
     1861 ccgaaaccgc ctttaatgtt cacaacacct agaataataa taactaaagc gccgcctaat
     1921 aaaatgacgc cttgaatgaa atcactccaa accacacctt cgaaaccacc taaaaatgta
     1981 tataaaatac atagtaaacc aacgagtgat gcaacgatat aagggttcat gtctgataca
     2041 gatgtgattg ctaatgttgg taagtagata acaattgcaa cacgccctaa atggtaaacg
     2101 acaaataata atgagccaat gacacgtatg ctagggccaa atctagcttc taaatattca
     2161 tatgcagatg ttacctttaa ctttttaaag aaagggacat agaaataaat aagtaatgga
     2221 ataattgcga cgatagcaat gttaccagcg atatatgacc aatctgttaa aaatgctttc
     2281 tctggtgtcg acataaatgt aatcgcactt aacgtagtag cataaattga aaagccaact
     2341 acccaagatg gcaagcgacc acttgcggta aagaaactat tggtactttg gctcgcgcgc
     2401 ttggtaaaat aaacgccaat gaacaacata gctagtagat aaatgataac ggcaacccag
     2461 tttagtgtgc caaatccaac ttctttcatg ggcaacatcc cctttacaat gtattgattc
     2521 tttgatgtct ataaatcgta ttttgcaatg agttgatcta atgtttgtcg atgtgcttcg
     2581 ttaaaaggtt tgaaaggtct tttcggtaat cctgcatcaa tgccacgatg acgtaatatt
     2641 tctttcaatg ttggataaat ccccattgat aacactgttt cgataatgtc gtttgaatca
     2701 tgttgcagtt ggtaagcttc ttgaatttga ccttgtcgtg ctaagtcgaa gatttttctt
     2761 gcacggcgac cattaacgtt atatgtagaa ccaattgcac catctacgcc agaaatcgta
     2821 gcttgaacta acatttcatc aaagccagat aagattaatt tgtctgggaa tgcttttcta
     2881 atacgttcga gtaggaagaa gtttggcgct gtatatttaa caccaacaat tttttcatga
     2941 ttaaatagct cgctgaattg ttcaatagaa atattcacac ctgttaaatc tggtattgca
     3001 taaataatca tattgttctg agttgcttcg ataatatcga aatagtaatc tctaatttct
     3061 tcaaaagtaa atggatagta gaatggtgtt acggcagaaa gtgcatcata accgagttct
     3121 gtggcatatt ttccaagttc aatggcttca tttaaatcta acgaacctac ttgagcaatc
     3181 aatttcactt tatccccaac tgcctctttg gcaaccttga aaacttgctt cttctgctct
     3241 gtatttaata aaaagttttc gcctgagcta ccatttacat aaagaccgtc taattcttca
     3301 gtttcaatgg cattttgagc aatttgttta agtccttgtt catttacttg accattttca
     3361 tcaaaaggaa cgagtaacgc tgcatataaa ccttttaaat ctttgttcat tatgaagtcc
     3421 ctccaaaaat catttgataa tatagtttac agctataatt gtaaacgcta tcataaaatg
     3481 taacaatatc tttttgaaaa ttgtagtcat atttatgtat aattaatgaa aatgtttttc
     3541 aaaatcaata gaaatggagt gagtaaggtg tattacatcg caatcgatat tggaggcact
     3601 caaattaaat cggcagttat tgataagcaa ttgaatatgt ttgactatca acaaatatca
     3661 acgccggaca acaaaagtga gcttattact gacaaagtat atgagattgt aacaggatat
     3721 atgaagcaat atcagttgat ccaacctgtc ataggtattt catcagcagg cgttgttgat
     3781 gaacaaaaag gcgaaattgt atacgcaggg ccaaccattc cgaattataa aggtactaat
     3841 tttaagcgat tattaaaatc actgtctcct tatgtcaaag taaaaaatga tgtaaacgct
     3901 gcattactag gcgaattgaa attacatcaa tatcaagcag aacggatctt ttgtatgacg
     3961 cttggtacag gcattggggg tgcgtacaag aataatcaag gtcatattga taatggtgag
     4021 cttcataagg caaatgaagt tgggtattta ttgtatcgtc caactgaaaa tacaacgttt
     4081 gagcaacgtg ctgcaacgag tgcattgaaa aagcgcatga ttgccggagg atttacgaga
     4141 agcacacatg tgccagtatt gtttgaagca gctgaagaag gtgatgatat tgcaaaacaa
     4201 atattgaatg agtgggcaga agatgtagca gaagggattg cccaaataca ggtcatgtat
     4261 gatccagggc ttatattaat tgggggcggt atatctgaac aaggagataa tctcattaaa
     4321 tatatcgagc cgaaagttgc acactattta ccaaaagact atgtttatgc accaatacaa
     4381 acgactaaga gtaaaaatga tgcagcatta tatggctgtt tgcaatgata gttgaaagaa
     4441 ggagtcattc taaaatagaa tttgaaaccg ttacgagaga tgagagctgt tgttagttcc
     4501 acacatcaca ctctatctag gaccaatcta aactatatca accaacagtg tgccacgggc
     4561 aaattaaatt gaagaagctg agatattaaa attttagaaa atgtaaaaaa atatttggta
     4621 ttgaaattaa aaaagcacct agcaactcgt tgggacaatc acgatgattg tctacagttg
     4681 caggtggatt tgaatatact actagttatt tgttgtctag gataatagat ttagtatgtt
     4741 gataagtttg actcagattc gtattttcta ataaatgata actcacgata tcgattaaaa
     4801 agagtgtcgc aatttgtgtg ttgataaatt gatggtcggt attacgcgat tgatccgttg
     4861 ttaaaagtac taaatctgca caatctgtaa gtttactacc ttcaaaattt gtgatggcaa
     4921 cgacatatgc accatgagat ttggcgactt ccgctgcaga aattaattcc gaagtattac
     4981 cactatttga catagcaata aacatatccg aatgagatag tagggatgcc gatattttca
     5041 ttaaatgtga atcggtagta acattacctt ttagccccat acgaatcata cgataataaa
     5101 attcagtcgc tgataaacca gagctaccta gtccagcaaa gagtatatgt cgacttgatt
     5161 gaagtttgtc gataaaggtt tggataatgt cgttatcaat aaattcacca gtttgttgaa
     5221 tgatttgttg atgatattta tgaattcttt gaataattgg gctattttca ataactgtct
     5281 ctgtcatttc ttgttgaata ttaaatttta aatcttggaa attctcataa tccagcttat
     5341 gactaaagcg tgtcatcgtt gctggtgatg taccaatcgc atgggctaag gagttaatcg
     5401 ttgaaaaggc atcgctataa ccattttgtc ttatataatt gacgatgcgt ttatcagttt
     5461 ttgtaaataa atgttgataa cgttgaacac gattctcaaa tttcattgtg tcaccccttc
     5521 atcttaatga ttactattat atatgaaaaa tattttcaag atagtaaaaa gcattgataa
     5581 aaattatctt aatgatatat tgtaaatgac tttacgtgaa aaaacgactt atggagtgag
     5641 gaataatgtt accacatgga ttaatagtat cttgtcaggc actaccagat gaaccattgc
     5701 attcatcttt tattatgtcg aaaatggcat tagctgcgta tgaaggtggt gctgttggta
     5761 ttcgcgcaaa tactaaggaa gacattttag caattaaaga aacggtagat ttaccagtta
     5821 ttggcattgt gaaacgtgac tatgatcact cagatgtttt cattactgca acgtcaaaag
     5881 aagttgatga actgatagaa agccaatgtg aagtcattgc attggatgca acgttacagc
     5941 aacgtccgaa agaaacgtta gacgaattag tatcatatat tagaacacat gcaccgaacg
     6001 ttgaaatcat ggctgatatc gcgaccgttg aagaagctaa aaatgccgca cgacttggct
     6061 ttgattatat tggcacgacg ttacatggct atactagtta tacgcaagga caattacttt
     6121 atcaaaatga cttccaattt ttaaaagatg tactacaaag tgttgatgca aaagttattg
     6181 cggaaggtaa tgtcattaca ccggatatgt ataaacgtgt gatggactta ggcgttcatt
     6241 gttcagtcgt tggtggtgcg ataacacgac caaaagaaat tacgaaacgt tttgttcaaa
     6301 ttatggaaga ttaaatgata acgataaaaa aacgagatga ccatcattaa ttaaaggcac
     6361 ctaattatct taggtggctg aatgaatgta atgggttcat ctcgttttgt ttgtttatga
     6421 tagtgatttt attttcaact ttatccaaaa ataagtaaag cgacggggat ggtgattaat
     6481 agcgacaacg ccacgcgtaa aaaccaaatg atgatgagtt tccagacagg tattttaatt
     6541 tcagttgcta gtatacatgg cactaatgct gagaaaaaga taatggctga tacgcttact
     6601 acaccgacga caaatttagt actcattgca gctttagtta ctaacaaaga tggtagaaac
     6661 atctctacaa tagaaatcgc tgacgctttt gctagtaaag cctgatcagc aattgggaaa
     6721 atataaataa atggatagaa gatatagcca agccaatcaa tgaatggtgt atagttcgct
     6781 acaatcagtc ctaaaaaacc aatcgataat atagaaggta aaataccaac agtcatttct
     6841 aaaccgtctt tcaaattgtc ccaaacgttc ttcacgagag atggtgttaa tgcattttgt
     6901 ttcatcgcct ctgcatatgc agttttcagt ctgcttcctt caatagcaac ttcttgttct
     6961 ccttcttgtc cgttataata ttctgttgat tcattgctga ttggcggtag ccatgcagta
     7021 attgcagtca cgacaaatgt gatgactaaa gttatccaaa agtataaatt ccaatgcggc
     7081 attaatccta aagttttagc aacgataatc ataaaagttg ctgaaactgt tgaaaagcca
     7141 gtcgcaataa tcgtggcttc tcgtttgttg tacatccctt gcttatagac acgattagta
     7201 atcaataatc ctaaggaata actgccgaca aacgaagcca ctgcatcgac agcggatttt
     7261 cctggtgttt taaaaatagg tctcataata ggctccatat aaacaccgac aaattctaat
     7321 aagccatagc ccactaataa agaaagcgca attgcaccta ctggaattaa gatacttaat
     7381 ggcatcatta atttttcaaa caaaaacgga ccatagttag ctttaaatag tattgatgga
     7441 ccgattttaa atacatacat tataccgatc attgcacctg caactttaaa taatgtaatg
     7501 accaagtttg tgattgaagt cataaaagta cgtctcacta ttggtaacgc tgtaccaatt
     7561 aaaatcataa tcagtgcaac atagggcata agtggaccta tgattgagcg aatggctaga
     7621 tgaacatgat cgacgaaaat agtgttgtta ccattaatcg taaaaggaat aaagaaacat
     7681 agtatgccca ctaaactata gacaaaaaaa cgccatgcac ttggttgttg tgcattagaa
     7741 tgatattgat tcattaaagc aacccctttg tttaaatgaa tacacaaaac tgtatgatgc
     7801 atcttcccct
//
LOCUS       DAKTGS010000131         7807 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.131, whole genome shotgun sequence.
ACCESSION   DAKTGS010000131 DAKTGS010000000
VERSION     DAKTGS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.131"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            328..1503
                     /gene="rpsA"
                     /locus_tag="O0286_002333"
     CDS             328..1503
                     /gene="rpsA"
                     /locus_tag="O0286_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCY0170680.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1725..3035
                     /gene="der"
                     /locus_tag="O0286_002334"
     CDS             1725..3035
                     /gene="der"
                     /locus_tag="O0286_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCY0170681.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            3052..4050
                     /locus_tag="O0286_002335"
     CDS             3052..4050
                     /locus_tag="O0286_002335"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCY0170682.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4221..4493
                     /locus_tag="O0286_002336"
     CDS             4221..4493
                     /locus_tag="O0286_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCY0170683.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            4924..5496
                     /locus_tag="O0286_002337"
     CDS             4924..5496
                     /locus_tag="O0286_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCY0170684.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            5499..6224
                     /locus_tag="O0286_002338"
     CDS             5499..6224
                     /locus_tag="O0286_002338"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCY0170685.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            6226..7185
                     /locus_tag="O0286_002339"
     CDS             6226..7185
                     /locus_tag="O0286_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0170686.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            7277..7726
                     /gene="ndk"
                     /locus_tag="O0286_002340"
     CDS             7277..7726
                     /gene="ndk"
                     /locus_tag="O0286_002340"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCY0170687.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         2855 a         1030 c         1507 g         2415 t
ORIGIN      
        1 cttatttgcg tggctttttg ttttataaaa gtataaaatt ttactataat atatcttgta
       61 gagaacaatg aaatgacaga cttataaaac aaaatagtaa gctatgctaa tttttataca
      121 aaaaatatgt ataaacatag caaactgtat tactttgata caaaaatggt tgtaataaat
      181 atttatcgat atgacgactt gaatatgata aagtgacata tttatgtata tgactatttc
      241 gcaaaatgta atcgaggtag aatttcttga caattctgtc agtttataag atgttataaa
      301 tatgtagtgt ataaggaggc aaacaagatg actgaagaat tcaatgaatc aatgattaac
      361 gatattaaag aaggtgacaa agtcactggc gaggtacaac aagttgaaga caagcaagtt
      421 gttgttcata tcaacggtgg taaatttaat gggattattc ctattagtca attatctacg
      481 catcatattg atagcccaag tgaagttgta aaagagggcg acgaagttga agcatatgtc
      541 actaaagttg agtttgatga agaaaatgaa actggagctt acatcttatc tagaagacaa
      601 cttgaaactg agaagtctta tagttattta caagaaaaat tagataataa tgaaatcatc
      661 gaagcgaaag taacagaagt agttaaaggt ggtttggttg ttgatgtagg acaaagaggt
      721 tttgttccgg cttcactaat ttcaacagac ttcattgagg atttctctgt gtttgatgga
      781 caaacaattc gtattaaagt tgaagaattg gatcctgaaa ataatagagt cattttaagc
      841 cgtaaagcag ttgaacaaga agaaaacgat gctaaaaaag atcaattatt acaatcttta
      901 aatgaaggcg atgttattga tggtaaagta gcgcgtttaa ctcaatttgg tgcatttata
      961 gacattggcg gtgttgatgg tttagtgcat gtatctgaac tttctcacga acatgttcaa
     1021 acaccagaag aagtagtttc aattggtcaa gatgttaaag ttaaaattaa atctattgat
     1081 agagatacag aacgtatttc attatcaatc aaagatacgt taccaacacc tttcgaaaat
     1141 attaaaggtc aattccacga aaatgatgtc attgaaggtg tcgtagtaag attggcaaac
     1201 tttggtgcat ttgttgaaat tgcaccaggt gtacaaggac ttgtacatat ttctgaaatt
     1261 gcacacaaac acattggtac gccaggtgaa gtgttagaac ctggtcaaca agtaaatgtt
     1321 aaaatattag gtattgatga agagaatgaa agagtatcac tatctattaa agcaacatta
     1381 ccaaacgaag atgttgttga aagtgatcct tctacgacta aggcgtactt agaaaacgaa
     1441 gaagaagata atccaacaat tggcgatatg attggtgata aacttaaaaa tcttaaacta
     1501 taatttaata tttaatagtc aactccacat gtttatgatt gcatgtggag tatttttatg
     1561 taacaaaata tactcggaat gataacgtgg acaaatttaa ctaagtgttt aaaaagataa
     1621 agttttaagt gctgattttt atcattacag taataaactc attttgaata cacagtctca
     1681 tgtgatatta ttaaaaagat ataagaaaga gaggaagtta gcttatgact aaacctatag
     1741 tagctattgt aggtaggcct aatgtaggta aatctacaat ttttaataga atagttggag
     1801 aacgtgtttc gattgtggaa gacacgccag gtgtaacacg agatcgtatt tattcttcag
     1861 gtgaatggtt aacacatgat ttcaatatta ttgatacagg tggtattgaa attggtgatg
     1921 caccattcca aacacaaatt agagcgcagg cagaaatcgc catagatgaa gcgtatgtta
     1981 ttatttttat ggttaacgtg cgtgaaggat tgacacaaag cgatgaaatg gtcgctcaaa
     2041 ttttatacaa atctaaaaaa ccggtcgtat tagcggttaa caaagtagat aatatggaaa
     2101 tgcgtacaga cgtgtatgat ttctattcat taggatttgg tgaaccgtat ccgatatcag
     2161 ggtcacatgg tttaggtctt ggtgacttgt tagatgcagt tgtttctcat tttggtgaag
     2221 aggaagaaga tccttatgat gaagatacaa ttcgactatc cattattgga cgaccaaacg
     2281 taggtaaatc aagtttagta aatgctattt taggtgaaga tcgcgttatc gtttctaatg
     2341 ttgcagggac aacgagagac gctattgata cagagtatag ttatgatgga caagattatg
     2401 ttttaatcga tactgctggt atgcgtaaaa aaggaaaagt atatgaatca actgagaaat
     2461 attcagtatt aagagcttta aaagcgattg aacgttcaaa tgttgtttta gtggtcatag
     2521 atgcagaaca aggcatcatt gaacaagata aacgtgttgc aggatatgca catgaacaag
     2581 gtaaagcagt cgtgattgtc gtaaataaat gggatactgt ggaaaaagat agtaaaacga
     2641 tgaagaaatt tgaagatgaa gtacgtaaag aattccaatt tttagattat gcacaaattg
     2701 cttttgtgtc tgctaaagaa cgcacaagat tacgtacatt attcccttac atcaatgaag
     2761 caagtgaaaa ccataaaaaa cgtgttcaaa gttcaacttt aaatgaagtt gttactggtg
     2821 caatttccat gaaccctaca ccaacagaca aaggtagacg tttgaatgtc ttttatgcaa
     2881 cacaagttgc tatagaacca ccgacatttg ttgtatttgt taatgatgta gaattaatgc
     2941 atttttctta taaacgctat ttagagaatc aaatccgtgc cgcttttggt tttgaaggta
     3001 caccaattca tattatagct cgaaagagaa attaacgatt gggggataac aatgactaaa
     3061 attaccgttt ttggtatggg aagttttggg acagcccttg ccaatgttct tgcagaaaat
     3121 ggacatgatg ttttgatgtg gggtaaaaat caagatgctg ttgatgaatt aaatacatgt
     3181 catacaaata aaaagtattt aaaatacgcg aaattagatg ttaacatcat cgctacttca
     3241 gatatgacca aagcaattca atttgcagat atttacttaa tggctttacc tactaaagca
     3301 atgcgagaag ttgcttctca aattaatgat aagctgacct ctaaaaagac ttttatacat
     3361 gttgctaaag gtattgaaaa tgggacgttt aaacgtgtgt cagaaatgat tgaagattct
     3421 atttcacctg aatataatgc aggtattggc gtgttgtcag ggccaagtca tgcggaagaa
     3481 gttgtagtca agcaaccaac tacagttgct gcttcatcaa aagataaaag tgtaagtaaa
     3541 ttaacgcaag atttatttat gaatgattat ttgcgtgtgt acacgaatga tgacttgatt
     3601 ggtgttgaac ttggtggtgc attgaaaaat atcatcgcag tagcaagtgg tatcgtagct
     3661 ggaattggct acggtgataa tgcaaaagct gcattaatga ctcgtggctt agcggaaatt
     3721 agtagattag gtgaaaagtt aggtgccgat cctatgacat ttctaggttt aggtggtatc
     3781 ggtgacttaa tcgttacttg cacatcaaca cattctcgga atttcacatt aggatataaa
     3841 cttggacaag gtgaatcaat ggatcaagca ttatctgaaa tgaatatggt tgttgaaggt
     3901 atttatacaa ctaaatcagt ttatcattta gctaaagaaa aaaatgtgga tatgccaatt
     3961 acaaatgcat tatatagagt attatttgaa aatatctcag taaaagaatg cgtaaaagat
     4021 ttaatggagc gcgataaaaa atctgaataa aatgtgtatt attacacata tacggctaat
     4081 aattacgtaa aatgaataga aatggcttaa attcaacgtt tttgccacat aaatgattgc
     4141 gatttatgta atgcttgtgt taaagtttta ccataatgat ataaacatat cattatcaaa
     4201 cctcaggagg tgaatgtcta atgaacaaaa cagatttaat caatgcagtt gcagagcaag
     4261 ctgatttaac taaaaaagaa gctggttcag cagtagatgc tgtattcgaa tcaatccaaa
     4321 actcacttgc taaaggtgaa aaagtacaat taattggttt cggtaacttt gaggtacgtg
     4381 aacgtgctgc acgtaaaggt cgtaaccctc aaactggtaa agaaattgat atcccagcaa
     4441 gtaaagttcc agcattcaaa gctggtaaag cattaaaaga tgctgtaaaa taattttact
     4501 taaaaagccc tgaataaggg ctttttattt tgcttttaat acttacaacc tgtacataaa
     4561 ttgtaatgtt cttctaagtt tttaatcttt ggaacattca ttgtttaagc gttcaaaata
     4621 ttacatgaca taatagtttt gataaaaata gcaactacaa aatatacata tgaatattga
     4681 gattaattgt tagcgttgaa tttacttaaa aggtaaccat gtctactata gtattttacg
     4741 ttatttaaaa agatgaataa tgtaaatgaa gtaaaggtta ttatgagaat tacaaaagct
     4801 acataaatta ctgttagttt aaattgaaat ttaaaaatga taagtaatct taatgcttca
     4861 aaagatttta atttcttaca tataatgtta atatgaatag tgataatatc gtgaggtgta
     4921 gacatggaaa caactgttag caaattggaa agacaaatag aagaaagatt aaagggcgta
     4981 tcagaatatg aatccattaa tattaatcat cggttaggta agttacttga tttatatgat
     5041 attccagatg ttgccaaagt ggcatgcctc acaattgaca catcaatgag acaccttgat
     5101 gacattacat ataatcattt gtctaaacat tccattttga ttggagattt aattagtgca
     5161 catttttata cgctactagc agaaatcaat gatttatcat ttcaaaatga aattagtaaa
     5221 gcaattgttg aaattaatga attgaaatct tcattacatc atcaagcttt aaatgattat
     5281 gaaatttctc aagcaatagt aaaaattgaa acacttttcc catatattac gttatctcat
     5341 ttcggtatca atattgatga gtcggaaata tataattatt tatttgaaga tatgtctgat
     5401 tactatccat catacttcaa gaaatataat caaagtgaag taaagcatta cttacacgat
     5461 atccaaaaaa gttatttaaa aagtagaggt aattaagcat ggctgacaat aaagcaaata
     5521 aagagcaagt acatcgcgtt tttcaaaata tttcaaaaaa atatgataga ttaaataata
     5581 ttattagttt tgagcagcat aaagtatgga gaaaacgcgt catgaaagac atgggtgtta
     5641 gaaaagggac gaaagcatta gatgtttgtt gtggtactgg tgattggaca atcgcattaa
     5701 gcaaagctgt aggaccaaca ggtgaagtta ctggtattga ctttagtgag aatatgttag
     5761 aagtaggaaa agaaaaaact gcttcaatgg aaaatgttaa acttgttcat ggtgatgcaa
     5821 tggaattgcc gtttgaagac aattcttttg attatgtaac aattgggttt ggattaagaa
     5881 atgtgccaga ctatttagtc gcgttaaaag aaatgaatag agtacttaaa ccaggtggta
     5941 tggtggtatg tcttgaaacg agccaaccaa ctttgccagt ctttaaacaa atgtatgcac
     6001 tatactttaa atttgttatg cctatttttg gaaaattatt tgcaaaatca aaagaagaat
     6061 atgaatggtt acagcaatct acgtttaatt ttcctggaaa agaagagttg aagcgcatgt
     6121 ttgaagaagc aggtttcata aatgtaagag tacgtagttt tacagggggc gttgctgcaa
     6181 tgcaccttgg ctataaagaa aaagataata ccaaaggtga ttaacgtggc aaagttaaac
     6241 atgaacaatg aaattaagaa agtggaacaa cgacttgaaa aggcaataaa aagtaaagat
     6301 tctgtattag aacaggcatc attacactta ttgtcttctg gtggtaaaag agtacgccca
     6361 gcatttgtta ttctgagtag ccaatttggc aaagatgaac aaacgtctga acaaacgtat
     6421 caagttgcag tcgcattaga gttaattcat atggcaacac ttgttcatga tgacgttatt
     6481 gataaaagcg acaagcgtcg aggcaagtta accatatcaa agaaatggga tcagacaact
     6541 gctattttaa ctgggaattt tttattggca ttaggacttg aacacttaat ggccgttaaa
     6601 gataatcgtg tacatcaatt gatatctgaa tctatcgttg atgtttgtag aggggaactt
     6661 ttccaatttc aagaccaatt taacagtcaa cagacaatta ttaattattt acgacgtatc
     6721 aatcgcaaaa cagcactgtt aattcaaata tcaactgaag ttggtgcaat tacttctcaa
     6781 tctgataaag agactgtacg aaaattgaaa atgattggtc attatatagg tatgagcttc
     6841 caaatcattg atgatgtatt agacttcaca agtaccgaaa agaaattagg taagccggtc
     6901 ggaagtgatt tgcttaatgg tcatattacg ttaccgattt tattagaaat gcgtaaaaat
     6961 ccagacttca aattgaaaat cgaacagtta cgtcgtgata gtgaacgcaa agaatttgaa
     7021 gaatgtatcc aaatcattag aaaatctgac agcatcgatg aggctaaggc agtaagttcg
     7081 aagtatttaa gtaaagcttt gaatttgatt tctgagttac cagatggaca tccgaaatca
     7141 ctacttttaa gtttgacgaa aaaaatgggt tcaagaaaca cgtagtattt atgaaaagta
     7201 ttgaaagcgc tttaccaacc tgttaatata taatagtaat atacataaac ctattaaaca
     7261 cagggggata cataaagtgg aacgtacatt tttaatgatt aaaccagatg cagtacaaag
     7321 aaatctaatt ggtgaagtaa tttcaagaat tgaaagaaaa ggactaaaac ttgtcggtgg
     7381 taaattaatg caagtaccaa tggaacttgc tgaaacacat tatggtgaac accaaggtaa
     7441 accattttat aatgatttaa tttcatttat tacatcagca ccagtgttcg caatggtagt
     7501 tgaaggtgaa gatgcagtta atgtatctag acatattatt ggcagcacca atccttcaga
     7561 agcttcacca ggatcaatta gaggtgattt aggtttaact gttggtagaa atatcattca
     7621 cggttcagat tcattagagt ctgctgaacg tgaaattaat ctatggttta atgaaaatga
     7681 aattactagc tatgcttcac cacgtgatgc atggttatat gaataaaata taaactgtaa
     7741 acctttacga tttatttata aaggtagaaa gggttttgtt atgtggttag tcattatgat
     7801 tatacat
//
LOCUS       DAKTGS010000132         7736 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.132, whole genome shotgun sequence.
ACCESSION   DAKTGS010000132 DAKTGS010000000
VERSION     DAKTGS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.132"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            222..623
                     /locus_tag="O0286_002341"
     CDS             222..623
                     /locus_tag="O0286_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0170688.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(886..2583)
                     /locus_tag="O0286_002342"
     CDS             complement(886..2583)
                     /locus_tag="O0286_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCY0170689.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            2858..3481
                     /gene="gmk"
                     /locus_tag="O0286_002343"
     CDS             2858..3481
                     /gene="gmk"
                     /locus_tag="O0286_002343"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCY0170690.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            3481..3699
                     /locus_tag="O0286_002344"
     CDS             3481..3699
                     /locus_tag="O0286_002344"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCY0170691.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            3915..5114
                     /gene="coaBC"
                     /locus_tag="O0286_002345"
     CDS             3915..5114
                     /gene="coaBC"
                     /locus_tag="O0286_002345"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCY0170692.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            5114..7522
                     /gene="priA"
                     /locus_tag="O0286_002346"
     CDS             5114..7522
                     /gene="priA"
                     /locus_tag="O0286_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCY0170693.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         2687 a         1121 c         1420 g         2508 t
ORIGIN      
        1 tgacaataag tcaaaacatt aatacctatg ataagtatca tttattaaca tatgtatcat
       61 atttttaatc ttgcgtaatt tttatcgtta actatgtgat ttaatcaaca ggccaataag
      121 acgtttgtca ttcgtgcaca taacggtacg acgcgctttt gtcattttaa ttatgttaag
      181 ataatagtag ttatagaagt tcaatctata ggaggcatag catggatatt ccaaaaatca
      241 cgacattttt aatgtttaat aaccaagctg aagaagctgt taaactatac acaagcttat
      301 ttgaagatag tgagattata acaatggcta agtatggtga aaatggacct ggtgatcccg
      361 ggactgtaca acactcaata tttacattaa atggacaagt attcatggcg attgatgcta
      421 atagtggcac agaattacca atgaatcctg cgatttcatt atttgttaca gtaaaagata
      481 ctattgaaat ggaacgacta tttaatggat taaaagatga aggtgccatt ttaatgccaa
      541 aaacgaatat gccaccatac agagagtttg cttgggttca agataagttt ggagtaagtt
      601 ttcaattagc attacctgag taaaaggatt cgcacagcgt tgaatgataa agaaacactt
      661 tttcttatgc atgcgtttta cctatgtatt tctattttaa gtacacatta gcattttagt
      721 ttcactcatt tttaaaatca caaagataat ttgtaactta aatgatgata cgttatttaa
      781 aaacacgata cttcgtttca atgaacgcat taaataataa ataaacacct caccataaga
      841 aaggactact ttcttttgtg aggtgtttat ttgtcgacaa cttgattatg attgtttcat
      901 tttttgaata agttcataat caggtgtagc atacaaagtt ttttgattgt catatgttac
      961 aaacccaggc tttgcacctg atggtttatg cacattttta attaatgtat aatcaacagg
     1021 tatttgtcca gaattaccag cttttgaaaa gtatcctgct aacatagccg cttccttgat
     1081 tgtcgtatca cttggtgcat cattaaatat aacgacatgt gaaccaggaa tatcttttgt
     1141 gtgtaaccat gtgtgagttt ttttagcttt tttatttgtt aaataatcat tttgcttgtt
     1201 attcttacca acatatatat cgtcgccatc agttgataca taatgttgta attgaatctg
     1261 cgctttcttt ttcttagttt gatttttacg ctgtttcata aagccttgtt ctgctaattc
     1321 atctctaatt tcatcaatgt catggacaga aatatgatgt aattgttgtt cgattgttga
     1381 aaaataatct atattgtctt tcgtcaattg aatttgatgt tgtaattcac gttctctcgt
     1441 tttcatacga ttatattgtt tataataata ttgagcattt gctgatgggg attttgtagg
     1501 atttaaagga atgacaactt cttcattcgt ataataattc aatgccgtca cttctttatc
     1561 gccttgctta attcgatata tattagcagt gatcaattca ccatataact gttcagtatc
     1621 tttattttta gactgctcat attcttcaat caacttcgct aatttatttt gatatttgta
     1681 caactgctgt tgaacaaatc gaactaaatc attcgcacgt tgtttaacgc gttcacgttc
     1741 gccacgcgca tcataaaaac gatcaagcaa atcatttaat gaatcgtatg taactgtatc
     1801 atcattaaat tgatttaact ttataaaata gaaatcctct ttacctgttt catgattttt
     1861 atgaaaaata ggagtaggtg gtaacttggt ttctgccatt acttcgtcaa atgcttctgg
     1921 taatgttgat gaagtaataa attgacgacg actaacgatt tcattcgtaa ttaaagggct
     1981 aaatccttca aactgattca ataattgttt agcaatatta cctgcgttaa aatcgatata
     2041 tttcaacacc tctgcacctg taatatcata cggatttatt ttgtgctgag taggtggtgc
     2101 ttcataatta aatcctggca ttactgtacg atagtgattc gtatttggtg ttaagtgttt
     2161 aaatccttca attattttgc gattttcatc tactaaaatt aagttactat gtttacccat
     2221 aatctcaagg atgacagtgc ggtaaatagt atcgccaatt tcatctttac tctttatatc
     2281 gatttcaatg cgacgatcat taccaatttg cttaatcgat tcgataatac caccttctaa
     2341 gtgttttcta aaaacacgcg caaacatggg tggattaaat ggattatcat attttttagt
     2401 agtcaattgt aatcttgaaa agtttggatg gattgacaat aacaattgat ggttttgtct
     2461 attttgacgt acaaccatta gtatcgtgtc attatcaggt tgattgattt tgtgaacgcg
     2521 tcctgttgtt aaaaattgta gagactcaac cattttcttt gtaaataagc catcataagc
     2581 cataatttat cttccacctt cttcatatca ttcgtttcat tttaacatga ttgtaacttt
     2641 tcagtatagt tattgaacca tacattatgc catacatttt acattatcta aaagtttgca
     2701 attcaattta tttaattaaa attaatcgtg caatataacg atattgttag acttaataga
     2761 aattatggca tgcaatttca aatatgctat acaatataaa gaacaatgtg atatcatatt
     2821 taaataatag aagattagct tagagaggtc gtaaggcatg gataatgaaa aaggattgtt
     2881 aatcgtttta tcaggaccat ctggagtagg taaaggtact gttagaaaac gaatatttga
     2941 agatccaagt acatcatata agtattctat ttcaatgaca acacgtcaaa tgcgtgaagg
     3001 tgaagttgat ggcgtagatt acttttttaa aactagggat gcgtttgaag ctttaatcaa
     3061 agatgaccaa tttatagaat atgctgaata tgtaggcaac tattatggta caccagttca
     3121 atatgttaaa gatacaatgg acgaaggtca tgatgtattt ttagaaattg aagtagaagg
     3181 tgcaaagcaa gttagaaaga aatttccaga tgcgctattt attttcttag cacctccaag
     3241 tttagaacac ttgagagagc gattagtagg tagaggaaca gaatctgatg agaaaataca
     3301 aagtcgtatt aacgaagcgc gtaaagaagt tgaaatgatg aatttatacg attacgttgt
     3361 agttaatgat gaagtagaac ttgcgaagaa tagaattcaa tgtattgtag aagctgagca
     3421 cttaaaaaga gagcgcgtag aagctaagta tagaaaaatg attttggagg ctaaaaaata
     3481 atgttaaatc cacctttaaa ccaattaacg tcacaaatta aatcaaagta tttaattgca
     3541 acaactgcag cgaaaagagc gcgtgaaatt gatgaacaac ctgaaactga attattaagt
     3601 gaatatcatt catttaaacc agttggtaga gcgttagaag aaattgctga cggtaaaatt
     3661 cgccctgtta tttcaagtga ttattatggt aaagaatagt ttcgaccatt aaaaatatgt
     3721 gaagctagac gtacatcaaa tacgtcaagg catatccaaa tcaaacgcac ctatgtaggt
     3781 gtgttttttt agtttatcca atgattgatg ttataataat actaaatttg tatctataaa
     3841 aaagtaatga gcatttgtgc gcatatgatg atgtaaagcg taaatgtcat agtgatattt
     3901 aagggagatt gaatatgaag aaaatattat tagccgttac aggtggcatt gcggcatata
     3961 aagcaattga tttgacaagt aagttaacac aatctgggta tgaagttcgc gttatgttaa
     4021 cgaatcacgc acaaaaattt gtgacaccat tagcatttca agcaataagt cgaaatgctg
     4081 tttatacaga tacttttata gaagaaaatc cttcagaaat acagcatatt gcattaggtg
     4141 attgggcaga tgcaatcatt gttgcacctg caacggcaaa tacaattgca aaattgagtg
     4201 taggtattgc tgatgatttg gtgacatcaa cgttgctagc aacagagaca ccgaaattta
     4261 ttgcgcctgc tatgaatgtg catatgtatg aaaataaacg tacgcagcaa aatattaata
     4321 ttttaaaaga agatgggtat cattttatcg aaccaggaag cggatttcta gcatgtggtt
     4381 atgttgctaa aggacgtatg gaagaaccgc ttcaaatcgt ttctgtaatt gatgctcatt
     4441 ttcaaaatag taatcgttta gctaatagtt catttcaaga taaacgcgca ttggttacag
     4501 caggaccaac tattgaagtt atcgatccag tcagatttgt atccaatcgt tcttctggaa
     4561 aaatgggcta tgcaatagct gaagcattgc gaaatcgagg agctatcgtg acgttagttg
     4621 ctggtcctac aacactagag gatccaaaag atattgaagt tattcatgtt caaagtgctg
     4681 aagaaatgtt tgaacaagtg acaagccgat ttgacgaaca agatattgtt gtaaaagcag
     4741 cagccgtatc tgactataca ccggttgatg tattagaaca taagatgaaa aagcaggatg
     4801 gtgatttgtc agtatctttt aaacgtacta aagacattct taaatattta ggtgaacata
     4861 aaacatcaca gtatttaata ggctttgcag cagagactga agatattgaa aattatgcac
     4921 aacaaaaatt acgcaagaaa aatgcagatg tgattatttc aaataatgtt ggggatatgt
     4981 ctatcggatt tagttctgat gataatgaat tgacaatgca ttttaaaaat aatgaaaaag
     5041 taaatatcaa gaaaggaaaa aaagtagtat tagctgcaca aattttagat gaactagaaa
     5101 ctaggtggca ataatgatag cgaaagtcat agtcgatgtc gcgtcgaaga gcgttgacta
     5161 taaatttgat tatataattc ccgaacaact cgaatctgtc atccaacctg gtgtgcgtgt
     5221 aattgtacct tttggaccaa gaacgattca aggttatgta atggaagtaa cagcagaacc
     5281 tgatgcacaa cttgacgttt cgaagttaaa aaaaatcata gaagtgaaag atatacaacc
     5341 agaattaaca tcagaattaa tagctttaag tgagtggatg ggttcaactc atgtcattaa
     5401 acgtatttct atgctagaag tgatgcttcc gagtgctatt aaagcgaagt ataaaaaagc
     5461 atttaagatg aaagatgaca tagagctacc ttcagcttta ttacaaaaat ttgataagca
     5521 tggttactat tattataaag atgcgcaaaa aaataatgat attcaattgc ttatgaagtt
     5581 gttaaaagat gatatcgttg aagaaaaaac gattctcaca caaaatataa ctaaaaaaac
     5641 caagcgtgct gttcgtgtca ttgaagggta tcatcctgat gaagtattag ctaagttgga
     5701 gaaagttatt aaacaatacg atttgtatgc ttacttgtct gaagaacaac ataaaacaat
     5761 atttttaact gatattgagg atatgggctt ttcaaaatcc agtttagatg gacttatcaa
     5821 aaaaggttat gttgaaaaat atgacgcggt tgttgaaaga gacccattta aagatcgtgt
     5881 tttcgaacaa gaatcaaaac agcaattaac agaagaccaa tataaagcat atgaagcgat
     5941 taaagctaaa attgtaagcc aagagcaaga aacattttta cttcatggtg tgacgggatc
     6001 aggtaaaaca gaagtatatt tacaaacgat agaagatgtt ttaagccaag gaaaacaggc
     6061 gatgatgtta gttcctgaaa tcgctctaac accgcaaatg gttttacgct tcaaacgtcg
     6121 atttggtgat gacgttgctg tattacattc tggcttatct aatggggaac gttatgatga
     6181 atggcaaaaa attagggatg gtcgtgcgag agtaagtgtt ggtgcaaggt caagtgtgtt
     6241 cgcacctttc aaaaatttag ggttaatcat cattgatgaa gaacatgaat ctacatataa
     6301 acaagaagat tatccgagat atcacgctag agaaattgcc caatggcgaa gtgaatatca
     6361 tcactgtcca gtcattttag gaagtgcaac accatgtctt gaaagttatg cacgagctga
     6421 aaaaggcgtt tatcatttgc tatcattacc aaacagagtg aaccaacaag ctttacctga
     6481 aattgatata gtagacatgc gtgaagaatt gagtgaaggt aatcggtcaa tgttttcaaa
     6541 agatttacgt gaagccatac aattaagatt agatcgacag gaacaagttg ttttattttt
     6601 aaatcgacgt ggttatgcat cgtttatgtt atgtcgggat tgtggatatg taccgcaatg
     6661 tccaaactgt gatatttcat taacgtatca taaaacgaca gacttattaa aatgtcacta
     6721 ttgtggttac caagagacgc caccgaatca atgtccaaat tgtgagagtg aacacattcg
     6781 acaagtaggt actggtactc agaaagttga agaactattg caacaagaat ttgaagatgc
     6841 gcgcataatt aggatggatg tagatacaac ctcaaagaaa ggtgcacatg aaaagttatt
     6901 gactgaattc gaaaaaggta acggtgacat tttactaggt actcagatga ttgcgaaagg
     6961 attagattat ccaaatatta ctttagttgg tgtgctgaat gcagatacaa tgttaaattt
     7021 acctgatttt cgggcgagcg aacgtactta tcaactatta acgcaagtgg ctggtagagc
     7081 tggtcgtcat gaaaaggcag gtcaagtcat cattcaaacg tataatccag atcattattc
     7141 aatattggat gttcaaaaaa atgattattt aacattttat cgtcaggaaa tggaatatcg
     7201 taaattagga aagtatccac cgtattatta tttgattaat ttcacaatct cacataaaga
     7261 aatgaagaag gttatggtag catcgcagca tgttcataaa attttattac agcatttaac
     7321 agaaaaagcg cttgtactag gtccatctcc ggcagcactt gcgagaatca acaatgaatt
     7381 tagattccaa attttagtga aatataaaag tgaacctgga ttattacaag ccattcagtt
     7441 tttagatgac tattaccatg aaaaatttat aaaagaaaaa ttagcattga agattgatat
     7501 tgatccacag atgatgatgt aacattacta attattagaa acaagttcag tatgtacgag
     7561 tatttgaacc agtgtttgaa tatttacttt aagtacagaa aaagggcaga atatacaact
     7621 gttaactatt taaattagca gtttatattc tgtcttttta tatggcttta taacttacgt
     7681 gattttggtt tgataaggaa tttattagta ttttcattta cgaattctga ttttaa
//
LOCUS       DAKTGS010000133         7716 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.133, whole genome shotgun sequence.
ACCESSION   DAKTGS010000133 DAKTGS010000000
VERSION     DAKTGS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.133"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..491)
                     /locus_tag="O0286_002347"
     CDS             complement(<1..491)
                     /locus_tag="O0286_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCY0170694.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSL"
     gene            582..1325
                     /locus_tag="O0286_002348"
     CDS             582..1325
                     /locus_tag="O0286_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0170695.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SESEEIMMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDSNYRLSNVDTMKVTLYS
                     NGSNYDKEALLINKDEFCPLRKITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPM
                     TFENKRDIVQDISKIFEVNYYTYNHIMHH"
     gene            1759..2361
                     /locus_tag="O0286_002349"
     CDS             1759..2361
                     /locus_tag="O0286_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170696.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            2628..5780
                     /locus_tag="O0286_002350"
     CDS             2628..5780
                     /locus_tag="O0286_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCY0170697.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            5837..6319
                     /locus_tag="O0286_002351"
     CDS             5837..6319
                     /locus_tag="O0286_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170698.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
     gene            6524..7510
                     /locus_tag="O0286_002352"
     CDS             6524..7510
                     /locus_tag="O0286_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCY0170699.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
BASE COUNT         2971 a          936 c         1345 g         2464 t
ORIGIN      
        1 ttgagactct ttggaagatt ttcaataaat gaatatttca caaacttcca cttatcagga
       61 ttataaatta taatttctgc aactgcatta tcgaagattc taaacaattt tttgatttct
      121 atttctttta gagataaagt agggaaaatg tcgatatatt cttcaagcgc ataaagacta
      181 tgtttaacag aatcggttat gttcattgaa tatatcacac caatatttat attttgccat
      241 ccaaaagaag aaataacatt atcataaaat ccattgaata taaattcagt catcccctct
      301 gaacttctcc ataaatataa tggtgaataa gtatttgtaa tgcaattagt agttgtttga
      361 cttattaaat aagcttttat aattaaattt ttaaatccat cagtcttata accatttaat
      421 cgaactctat ctctaattat ttccatattg taatcactag gcaacttaac atgatattgc
      481 attgcatgca tttagcatac ccccttttat aaaaaggata gcaataataa gtaaaatctc
      541 atattatcca attgtgatat agttatcata aaaagtgata ggtgattaaa ttgaacttta
      601 atgatttgga aatttttata actgtatgtg aagaagcatc tatcaataaa gctgcaatta
      661 aacttagata tgcacaatct aatatatctc aaagaattag caagcttgaa aatgaattag
      721 gtgtagtttt gctttttaga aatcaaaaag gtgctaaggc aactaaagca ggcgaagaat
      781 tcttagcgta tagcaaaaaa gtattaagag atacagagac tataaaaaat aaaatgaaaa
      841 ataatactat gtctatttta tgctcagaac tgttatttaa ttatttatct gagagcgaag
      901 aaattatgat gtcgaataac tcaattaatt ttatttctag tggaaatatt agaaaagcta
      961 tagaaaaaaa taattatgat aaggttattt cattcataaa aattaacgac tcaaattata
     1021 gacttagtaa tgttgatact atgaaagtaa cgctttacag taatggaagt aattatgata
     1081 aagaggcttt actaataaat aaagatgagt tttgtccttt aaggaaaata actttagata
     1141 ataagcttga ttcacaacgg gtaatggaaa ttgattcatt ggcagcaata ataaacttag
     1201 ttaaacaagg aaaaggaaag gctttattac ctatgacttt tgagaataaa agagatatag
     1261 tacaggacat ttctaagata tttgaagtta actactatac ttataatcat attatgcatc
     1321 attaattgaa agttgattga gtttaaatta caggaacgat tttaaaagtc tagtagttag
     1381 aatgagcgat gtgacagaaa agaaattatg ccttttattt tgtaaattcg ttgttttaaa
     1441 gtaaaggtcc ttgtagtata ggaaaaagtg gatgacttct ccgcctttac caccctttca
     1501 agcataatga taactgctaa atattgatta tagcaccatt gcaaaactaa tcattaattg
     1561 tcatattcaa ctattttgaa aagcatcaat ttcacattat caaattattg agtatctcaa
     1621 tttattgtta tacacaaaca gtatttttgt tatcatagag ttaataaata taataaatta
     1681 aacaagtaac tttacaaaaa ttagaatgat tcaattcaat cataattgtt tgaaaataga
     1741 ggtgagcagg tgaacgatat gttaattagt cttgtaatcc cagtttttgc tttgttagtt
     1801 attggtggta ttatttggat gattatagaa ggtatagtac atatttcaaa aaagaataaa
     1861 gcaattgata acttttttaa ccaagttaat aaagtaagtg agacatataa attcgctact
     1921 acttttttat ttctaatctt ggctacggct ggtatttctc aattttatct atattatata
     1981 gtatcagcgt ttcttttttg gcttgtcata cttacctttg gcattgcagg tatcattttt
     2041 ttaatgccat atggattatg ttttctaccg ttttataagc aaaaaaagaa aaaacagaca
     2101 tttaaaaaat acatggttta cactacgatt ggtttgtcaa tttgtctagg cttatctcta
     2161 gttttggttc acactacgaa aatttatatg gacgaaggtg gcgtaagata ctattacggt
     2221 agttttgtaa tgaaacaagc gggcggttat gcttatttag ctttagcggt actttcaacg
     2281 ttgttaattg ttgcgaaaaa agctacaaat aaaaataaag aaatcgaaac cgtcgacaat
     2341 acaaatataa cggaaagata attaagggag tgctcattca ggagtgctct tttttgatgt
     2401 ccaaatttag ttgcaaatga aggcatagaa gaaattttta tagtcattta ttggataatg
     2461 aattatggtc atatcgatta attttattag tgaggatttt acaaacattt gttgaataaa
     2521 tatggtaatg ataatatatg aatatttttg aaaaaacact aatatgatta aattatttgt
     2581 taaaatagca ttaagtaaaa aacaaatagg gaacaaggtg ggatttcatg agtcaattgc
     2641 taaatgatac gttatcggct tggttgttaa ttgaatcttt aagtccagga gaagtaaatt
     2701 ttacagcgga agatatactc tcagctgaac attttaaaaa tggtgcaaag caagcacaac
     2761 ttcaaagttt tgatgaatat tttgaaatat ggaatgctga acgctttatt atatcagaag
     2821 aaaaatcaga gactggggaa cttatattta aattttatag acattgcttc cgctataatg
     2881 aaattaattt gaaaattcag gatatttttg atgattattc tgagattcat aatccaaatg
     2941 ggacacactg ttatggttac acatttaaca cagataaaca cggcaaagtg atagttgatt
     3001 ctatacatat tccgatgatt atgagtgcat taaaagaaat tgaaaagaac aaaaatgcca
     3061 atatagaaga aaaatttaat gattctgttg aaaaatttgt tcaaaaagta aaagaaattt
     3121 tagcagatga accaattaat gaatttaaat tgaagaagat ggacaaagct tatgatgagt
     3181 acttttctgt attaaattca aagaaagatg gattatttgg acattatgta gcaatagaat
     3241 atgtgaaaga tagtgattta ccacagccgg aatttaacag cttcttcata agtgatattg
     3301 agaaagcaag aaaatctccc aaccaaactt taattgatta cattgaaggt gtagaagaaa
     3361 gtcagcacat agaagtagat gaaaataaag aaatgtttga caaattttta catccttcac
     3421 gtttgcctga tggacgatgg ccatcacaga ctgagtttag attgtcttta atgcaacaac
     3481 ttgctgtaaa ccaaattacg agtggtaatg aaagaataag ttcagttaat gggccaccag
     3541 gtacaggtaa gactacttta ttaaaagata tatttgctca tctagtagtt gaaagaggta
     3601 aagagttagc taaactaaat aatcctaaag atgcatttgt caaaacaaaa attcatgaaa
     3661 cggatgataa atacgtatac ttactaaagg aatctattgc caaatataag atggtagtcg
     3721 catctagtaa taatggagct gttgaaaata tatctaaaga tttaccgaaa attgaagaaa
     3781 ttataagaaa tcccgaaaaa tgtaaattcc ctaaatatga acagaattat gcaaatttag
     3841 cacatgaatt aaaagatttt gctgaaatag ctgaagattt gattggtgaa agtgcctggg
     3901 gcttattttc tggagttttt ggtaaaagta ctaatattaa ccaagtattg agtcatatgt
     3961 taaaacaaga tgcgaatgat attggctttg ctaaattact acaaaatgag aataatcgta
     4021 tgagttataa cgagttaatg agtgaatggc aatcacatca acgtgcattt ttagaagagt
     4081 tgaggcatgt tgaaatgtta aaagaagaat ctattagagc atatgatgtt tataaaaatt
     4141 gtgagtcttt ctctaagatt gaacaggtta ttaatagtga aaaaacaagt attgaagaac
     4201 aggtatatca tttagataat gaaacgttac gagacaataa agaaatagaa gatttggata
     4261 atcgaattaa ttatattgtt aagcaaatag aaactttaaa tgagttaatt aaatccatca
     4321 aagaaagcaa caaaggtttt attaacaaac tgaaagcgat gtttaattca gaagaagatg
     4381 aaagctataa agatcataat aaagagaagc aacaattatt aacacaacag ttagagttag
     4441 agaaatgtaa aaaaaacaaa catgaagacc ttgttagcaa actaaaagaa aaagagaaat
     4501 taattaaaca attaactaaa gtacagttgc aattagacga gttaaattca cagttacaag
     4561 agttagaagc atatcgtatt gagtcaaaaa ttacaattcc agaaaaagat ttttggagtg
     4621 acaacaatta tgatgagcgc caagttacta atctgtggac gagtgacgaa cttcaataca
     4681 gacgtgccat gctcttttta agagcaatga tattgcataa attattattg attgctaata
     4741 atacaactat ttattatgcg attaatgatt ttaaagatag aaggaaatta attgatgcaa
     4801 atccagataa agtacacaac gcatggaatg tgatgcattt aatatttcca gtagttagta
     4861 cgacgtttgc aagctttaaa tctatgtatg ggggcatacc aaaagatttc atagactact
     4921 tatttattga tgaagcagga caagcaatac ctcaagcagc tgtgggagca ttatatcgtt
     4981 caaaaaaagt tgtagctgta ggtgatccga ttcaaataga accggttgtg actttagaaa
     5041 gtcatttaat tgataacatt cgtaaaaatt atcatgttcc ggaatatcta gtttctaaag
     5101 aagcttctgt gcagtctgtt gcagacaacg ccaatcaata tggtttttgg aaatctgatg
     5161 ctactgatag taatcaaaaa acctggatag gcataccttt atgggtgcac agacgatgtt
     5221 taaaacctat gttcacgata gctaaccaaa tcgcttataa taataaaatg gtgttgccaa
     5281 gtaatattac aaaagtaggt aaaacaggtt ggtatgacgt taaaggaaac gcagttcaaa
     5341 aacaatttgt gaaagagcat ggtgaaaaag tagtgggatt attagctgat gattggattg
     5401 aagcaattaa ggaaggtaaa aatgaaccga gctcatttgt aatatcgcct ttttcagcag
     5461 tacagcaaca gattaaacgt atgttaaagc aacaactacc gactagaatt gatattgaac
     5521 gtacaaaaat taatcaatgg gtcgataaat ccattggtac tgttcatact tttcaaggta
     5581 aagaggctca gaaggtgtat tttgtaatag gtactgataa tacccaagat ggtgctgtga
     5641 actggtcatg cgaaaaacca aacttgttaa acgttgcagt gacaagagct aagaaagagt
     5701 tttatgtaat tggcgacatg caaagaatac agatgaaacc attttatgag acgattttta
     5761 aagaaagaaa tgtaaaataa catacaaaaa agtatataga ggaagttata cattttaaaa
     5821 ggagcaaaat tgaataatgg agaaatttaa caactggata ttaaatgcaa taagtggatc
     5881 tcaaacagac aagaatgaaa caactgaaga attaaaaggg gcaaaattta tcattttata
     5941 tgcatattca atgctcgttt tgcttgcgtt agtaatttct aacatattca ttcacatttt
     6001 ggagcctaaa ctatcaatca ccactcaaat catcatcgtt ttgattttaa ttgaagcact
     6061 aattggactg cgtttcttga aagcgtacga tgttaagcgt ggcaaagata aagaaaataa
     6121 gaaaaatagt aaggatttcg ttaaactaaa atcaatttta gtagcaattt tatttacatc
     6181 attggcgctg acagcaggta ctgtagctga tatatacggt ttcactgact taggaaatac
     6241 tagaagtgat ttaatcgttt ggagcatagg tggtattata tttggcctcg tatgttacac
     6301 aatggaagat aaaagataac gataaggagc tggcgattat aaagctagct ccttttttaa
     6361 cttatatatg taaagaacta tcctaagggt ttttaatcat atgtcaataa tttctataat
     6421 acattattaa aacatcaatt aaataagttt taaaatttta cacatatttt tatttaaaaa
     6481 agatgtataa ttaatgtatt aaatatagaa agaagttgat attatgaaaa agtgtattaa
     6541 gactttgttt ttaagtatca ttttagtagt gatgagtggt tggtatcatt cagcacatgc
     6601 gtcagattcg ttgagtaaaa gtccagaaaa ttggatgagt aaacttgatg atggaaaaca
     6661 tttaactgag attaatatac cgggttcaca tgatagtggc tcattcactt taaaggatcc
     6721 agtaaaatca gtttgggcaa agactcaaga taaagattac cttacccaaa tgaagtcggg
     6781 agtcaggttt tttgatatta gaggtagagc aagtgctgat aatatgattt cagttcatca
     6841 cggcatggtt tatttgcatc atgaattagg aaaatttctc gatgatgcta aatattactt
     6901 gagtgcttat ccaaacgaaa caattgtgat gtctatgaaa aaggactacg atagcgattc
     6961 taaagttacg aagacatttg aagaaatttt tagagaatat tattataata acccgcaata
     7021 tcagaatctt ttttacacag gaagtaatgc gaatcctact ttaaaagaaa cgaaaggtaa
     7081 aattgtccta ttcaatagaa tggggggtac gtacataaaa agtggttatg gtgctgacac
     7141 gtcaggtatt caatgggcag acaatgcgac atttgaaacg aaaattaata atggtagctt
     7201 aaatttaaaa gtacaagatg agtataaaga ttactatgat aaaaaagttg aagctgttaa
     7261 aaatttattg gctaaagcta aaacggatag taacaaagac aatgtatatg tgaatttctt
     7321 gagtgtagcg tctggaggca gcgcatttaa tagtacttat aactatgcat cacatataaa
     7381 tcctgaaatt gcaaaaacga ttaaagcaaa tgggaaagct agaacgggtt ggctgattgt
     7441 tgactatgca ggatatacgt ggcctggata tgatgatatc gtaagtgaaa ttatagatag
     7501 taataaataa ggattcaata atgatattaa gacgagtatg aaaatagtta gattctaatt
     7561 attttcacta ctcgttttta ttttgaaaat aagtaataat tcaacaatat tataaattga
     7621 acagattgtt tgtgaaattt ttgataatat taaagtgaaa aagtgttata aattgataaa
     7681 tatatgtaat taacaaaaac aaatcatttt aaaaag
//
LOCUS       DAKTGS010000134         7430 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.134, whole genome shotgun sequence.
ACCESSION   DAKTGS010000134 DAKTGS010000000
VERSION     DAKTGS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.134"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..2520
                     /locus_tag="O0286_002353"
     CDS             <1..2520
                     /locus_tag="O0286_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCY0170700.1"
                     /translation="YLNEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLA
                     EKINQEFEINDITKVKVYIHNLVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDDFVK
                     KIREIQKLILAYEKTEIGFGEELYKDIIHHMKALFKCKNYLQIDTKIDMINNYLHQDI
                     ATNISEAAYLLWLLSRNNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDITGFGTTIF
                     QEEETDGNNIVMLKQKFLHALRNNDEIVINEKDVESLINDNEINHYHAPMSADVYAEL
                     YLGRFYNQYNELIVISPLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYYQKMICDD
                     NVEMISINNIPKYPRNHNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVGATFNKLY
                     LYSSQLNKRVLFESNNMYNFLKECNLYRLLREISMESVKCIEPMNDVSIDSFSYSPRI
                     RYKNVILKPAYWKINEMVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDNKLLLNLS
                     LANHRYLLMKEYKKHKRVRLVESFLPQSKNDHVYEIVTPIYKKSSYRGPEIEIPKYKN
                     TDIEYDKDWFALHIHIDKPSQDTFIIDNLYPFVKHLKDKGDIDQYFLMRYIKQGDILK
                     LRLFRNDENYAEIYSILKNWLPHVRQTTEVSDYEFVSYEPEFFRYGGKNTINEIEAFF
                     EYDTNLAVNIIENDFKFDRPYIVAISIMYLFEMFSISNEERMEIVNNYVPTSFKSKDI
                     RPFKNELVTICNPANNFEYMAKHYSGIYRILKDGNQILSKLNEGLKKTLTTKRSRIIG
                     SLIHMRCNRIFGIDKDQETFVLSIVKEIVKTQKHWCGDKND"
     gene            2513..3757
                     /locus_tag="O0286_002354"
     CDS             2513..3757
                     /locus_tag="O0286_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HCY0170701.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            3773..4291
                     /locus_tag="O0286_002355"
     CDS             3773..4291
                     /locus_tag="O0286_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HCY0170702.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            4301..5674
                     /locus_tag="O0286_002356"
     CDS             4301..5674
                     /locus_tag="O0286_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCY0170703.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            5697..6389
                     /locus_tag="O0286_002357"
     CDS             5697..6389
                     /locus_tag="O0286_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HCY0170704.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            6386..7147
                     /locus_tag="O0286_002358"
     CDS             6386..7147
                     /locus_tag="O0286_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCY0170705.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            7144..>7430
                     /locus_tag="O0286_002359"
     CDS             7144..>7430
                     /locus_tag="O0286_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCY0170706.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNAS"
BASE COUNT         2813 a          932 c         1230 g         2455 t
ORIGIN      
        1 tatttaaatg aacaaagtgc tatttattta aacaacaata aagatacatc tttttccatt
       61 aaaaatagcg aattactcgt attcatcaaa accactgtga cgaataataa tattactttt
      121 tcaaatttag ctgaaaagat aaatcaagaa tttgaaatta atgatattac taaagtgaaa
      181 gtatatatcc ataatcttgt ctcaaaggaa attatatatt caacaatcag accaccgtta
      241 tcatatagcg ataatttaaa ttatatttta aataaacttt ctttacataa tgatgatttt
      301 gtgaaaaaaa ttagagaaat ccagaaattg atattagctt atgaaaaaac tgaaattgga
      361 tttggcgagg aattatataa agacattatt catcatatga aggcactatt caaatgtaaa
      421 aactatcttc aaattgatac caaaattgac atgattaaca attatttaca tcaagatata
      481 gcaacaaata tatctgaagc agcgtactta ttgtggcttt tatcaagaaa taatattgga
      541 ttcacagatt tgaaagttct tcacaaccgt tttattgaaa agtatggatt tgaacaactc
      601 gtgaatatta aagatttgat ttcagatata acaggtttcg ggacaacaat ttttcaagaa
      661 gaagaaaccg atggaaataa tattgtaatg cttaagcaaa aatttttaca tgccttgaga
      721 aataacgatg agatagtcat aaatgaaaaa gatgtagagt cattgattaa cgataatgaa
      781 atcaatcatt atcatgcgcc aatgagtgca gatgtttatg ctgaacttta tttaggacgt
      841 ttttacaatc agtacaatga gctcattgtt attagtcctt taacggcgtc ttttaatgct
      901 ggcgctacat ttgggcgatt tcatcattta attgatactg aaactttagc aaaattagaa
      961 catgaaaaag gacattatta tcagaagatg atatgtgatg acaatgtaga aatgatttct
     1021 ataaataaca taccgaaata tccgagaaat cataatgtat taactaatca tgactcatac
     1081 gaatattcat tgaatttagg aagtagtaat agttattcaa agtatgagct taccttagat
     1141 gatatttatg ttggtgctac ctttaacaaa ttatatttat attctagcca actaaataaa
     1201 agggtactat ttgaatcaaa caatatgtat aactttttaa aagaatgtaa tttatatcga
     1261 ttgttacgag aaatttcaat ggaatctgtg aaatgcattg aacctatgaa tgatgtgagt
     1321 attgattcat ttagttattc accacgaatc agatataaaa atgtcattct taaaccagca
     1381 tattggaaaa ttaacgagat ggtattacct ttacctaaaa atgaagaatg ggatcaacaa
     1441 tttttgaaat atcaagaaca attcaatata cctaatatcg tgaatttagt ttatggagat
     1501 aacaagcttc tactaaattt atcattagct aatcatagat atttattgat gaaagaatat
     1561 aagaagcata aacgtgtacg tttagtagaa tcatttttac ctcaatcaaa gaatgatcat
     1621 gtgtatgaaa ttgttacacc aatttataaa aaatcatctt atcgaggacc cgaaatcgaa
     1681 attccaaaat ataaaaatac agatattgaa tatgacaaag actggtttgc attacatatt
     1741 catatcgata agccatcgca agatacattt atcatcgaca atttgtatcc atttgtaaaa
     1801 catcttaaag ataagggaga tatagatcaa tattttttaa tgcgttatat caaacaaggt
     1861 gacattttaa agttgaggtt attcaggaat gacgaaaatt acgcagaaat atacagcatc
     1921 ttgaagaatt ggttaccaca tgttcgacaa acgacagaag tttcagatta tgaatttgta
     1981 tcatacgagc cagaattttt tagatatggt ggaaagaaca cgattaatga aatagaagcc
     2041 ttctttgaat atgacacgaa tttagctgtc aatattattg aaaatgattt taagtttgat
     2101 cgaccatata ttgttgctat ttcaataatg tatttatttg agatgttttc aatttctaat
     2161 gaagagcgta tggaaattgt taataactat gtacctacat catttaaaag taaagatatt
     2221 agacctttta aaaatgaact tgtaactata tgtaatcctg ctaataattt tgaatatatg
     2281 gctaaacact atagtggtat ttatcgaatt ttaaaagatg gcaaccaaat tttgagcaaa
     2341 ttaaatgaag ggcttaaaaa aactttaact actaaaaggt caaggattat tggcagctta
     2401 attcatatgc gctgcaatcg aatatttggc attgataagg atcaagaaac atttgtttta
     2461 tctattgtaa aagaaattgt taaaactcaa aagcattggt gtggtgacaa aaatgattga
     2521 tatgaatcta atattgaaga agaagtttca agagatttca gaagtagatg atttcatttc
     2581 aaaagcatct actgaaaccg attattttga accttcaact ttatcacatg gtataccagg
     2641 tatcattttg tttttagatg cttatcaaaa agttttcgat attaatactg agcaaattgt
     2701 gcataagtac atcatgaaat tagcaccata tttacagagt ggtcaatata acaattcttt
     2761 gtttggtgga ctttcaggta ttgcattttc aatggattta gcatcgcaaa atggcaaaaa
     2821 ttatcaaaat attttaaata atattgatgg tcacattgta aatgaaatag aaaataaatt
     2881 tgatcaaata ttacaagaac cattaaatcc attaaattat gatactgtca gtggattagc
     2941 tgggataggg agatatttgc taaatagagt agatgagaat gaatttaatg ttaaagcatt
     3001 aaaaagcata ttagtatact ttaaagatat tcaatattct aaaaatagct gggtagtccc
     3061 acaagaaagt caatttttag agtctgataa aaattatttt actgaaggta atatcaatct
     3121 tggccttgca catggagtgc taggaccgat gtctttattt gcattatgcg tgattaaagg
     3181 aattacgatt gaaaatcatc agcacatatt aaaagacatg tacaaattta tcatggacga
     3241 aaaattttgt aaccacgaaa gatggttgca gcgttacgat ttaatttctg aacgtaatca
     3301 tttcaatttt attcggaatg gttggtgtta tggcaatacg ggtgtaatga cgacgttgtt
     3361 tttaatcggc caagcattac aagatgatga aataattaaa atgtctaaaa aagtgatgct
     3421 acaagtagta aatgataaag atgaaaattt aataagtcca actatttgtc atggattgtc
     3481 atcacaaata ttaatgttaa caattatgaa tttgaatttt gaattaaatg aagtgtctga
     3541 ttatatcact gtattaataa ataaactgat ttctcattat aaggaagatt atctggtgaa
     3601 ttttatagac attaatgaaa ataagcaaga tgtatttaaa agtaggaaag ttggcctttt
     3661 agaaggtgaa ttaggggtct atctaacatt gatgacactg aaaaatacaa aaattctcaa
     3721 tgaaaaaaat tggaccaatg cgtttttaat tagctgaatg gaggcgtata tcatgggaga
     3781 aaatgtattg atttgtttgt gtggctcagt taatagtatc aatattagcc actatattat
     3841 tgaattaaaa tcaaaatttg atgaagtaaa tgtaatcgcg tcaacaaacg gtagaaagtt
     3901 tattaatggt gaaatattaa agcaattttg cgataactat tatgacgaat ttgaagaccc
     3961 ttttttaaat catgtagata tagcaaataa acatgacaag attattattt tacctgcgac
     4021 ttctaatacg attaataaaa ttgcaaatgg tatatgtgac aatttattat taactatttg
     4081 tcatacagct tttgaaaaac tttctatatt tccaaatatg aatttacgaa tgtgggaaaa
     4141 tccagttact caaaataata ttcgattatt aaaagattat ggtgtatcaa tatatccagc
     4201 aaatatttca gaaagttatg aattagcgtc aaaaacattt aaaaagaatg ttgtcgcacc
     4261 tgaaccatat aaagttctgg aattcatttg agattagaat atgaaaatca taaagcgcgc
     4321 tattattagt ttaataatat tgagtctttt aatatctatt acaatgagta atgcatcagc
     4381 gtcagaagaa ctatattaca gtgttgaata caaaaataca gcaacattta acaagttagt
     4441 taaaaagaag tccttaaacg ttgtctataa tataccggaa ttacatgtgg cacagattaa
     4501 aatgacgaaa atgcatgcta atgctttagc aaactataaa aatgatatta aatatatcaa
     4561 tgccacatgt tcaacttgta ttactagcga gaaaacaata gacagaacat caaatgagtc
     4621 attattttca agacaatggg atatgaataa aataaccaat aatggtgcat cgtatgatga
     4681 tttgccaaaa catgctaaca ccaaaatagc aatcatagat acaggtgtga tgaaaaacca
     4741 tgacgatttg aaaaataatt tctcgactga ttctaaaaat ttagtacctt taaacggttt
     4801 tagaggtact gaaccggagg aaacaggtga tgttcacgat gtcaatgata ggaaaggaca
     4861 tggcacgatg gtgtcgggtc aaacgagtgc taatggtaag ttaataggtg ttgcaccgaa
     4921 taacaaattt acaatgtatc gcgtgtttgg tagtaaaaaa acagaactgc tttgggtatc
     4981 aaaagcgatt gttcaagctg caaatgatgg aaatcaagtc attaatatta gtgttggtag
     5041 ttatattatt ttggacaaaa atgaccatca aacatttaga aaagatgaaa aagtagaata
     5101 cgatgcgtta cagaaagcaa tcaattacgc caagaagaaa aaatctatcg ttgttgctgc
     5161 agctggtaat gatggtattg atgtcaatga caaacagaaa ctaaaattac agcgtgaata
     5221 tcaaggtaat ggcgaagtga aagatgttcc tgcatctatg gacaatgtcg ttacagtagg
     5281 atctacagat caaaagagta atctatctga gttttccaat tttggtatga attatacaga
     5341 tattgctgcg cccggaggat catttgctta tttaaatcaa ttcggtgtgg ataaatggat
     5401 gaatgaaggg tatatgcata aggagaacat tttaactact gccaataacg gaagatatat
     5461 ttatcaagct ggaacttcat tagccacacc taaagtttcg ggagcactag ctttaatcat
     5521 tgataaatat catcttgaaa aacatccaga taaagcgatt gaattgttat atcagcatgg
     5581 gacatctaag aataataaac catttagtag atatgggcat ggtgagcttg atgtgtataa
     5641 agcattaaat gtagcaaatc aaaaagcaag ttaataaatc aaaggagttt ttgattatgg
     5701 caaaattagt tactgaaaac atttcgaagc ggtttaaaaa tcaagatgta ttaaagcata
     5761 ttaatatcac tttagaaaat aacgaagttt atggattact tggtattaat ggagccggta
     5821 aaacgacact tatgaaaatt atatgtggca tacttcaaca agattcaggg gaaattaaat
     5881 tagataatag accaatgaca cgaaatgatt tgcacaaagt tggttcgctt attgaaacac
     5941 ctgcgacata taatcattta agtgcacaag ataatttgaa aattgtgtgt ttaaatgaaa
     6001 gcgttgattt cagcgaaatt aatagtgttt taagcttagt caatttaaat gtcgataaaa
     6061 agaaaaaggt taaggacttt tctttaggta tgaaacaaag acttggaatt gcaatggcat
     6121 taattaaaaa gccagaaatt ttagtattag acgaaccatc taatggttta gacccatatg
     6181 gaatccaaga acttagagaa cttctaaaat tattaacaga acaaggtact agtattatta
     6241 tttcaagtca cattttatct gaaatccaag ttttagcaga tcatatcggt attattcatg
     6301 agggtgagct aaaatatcag caaagaaata acaaagatga aaacttagaa gagatattct
     6361 tcaaaataac gaaaggtgat tacaaatgat acatttaaag atagaaggta tcaaatttaa
     6421 aaattctttc agtatgtatg ttttattaat aagtccgctg gtatttcttt gttttgctat
     6481 tttcacagtc ttattcgcca aaagtaatac gggaacagcg aatagtgtgt caccatatat
     6541 aactttacta tttaatattt ggccaattgc tttcatcccg attgtattat gtatggcttg
     6601 taattcgtta tttaaaattg aaatgagaaa taaatcattt aattattact taagtaataa
     6661 ttggtcgatt acaaaagaaa taagagcaaa gattttcatt ttatcaatag catttttggt
     6721 acattgcttt ttagtattta ttattgctta tataggtgac ctaattatta atccacatcc
     6781 ggttaatgct atgttgttat tggtgacaat attgttgatg tatgtagtat ctctaccatt
     6841 gataccgctc aactttttat taactcgata ctttggtgtg ttcgtatcaa tattaataaa
     6901 cttagtatta tcagtcattt gtgtcttgtt tttaacattg aagagtttat tttgggtgtt
     6961 gccgtggggg ataatgcaga gaatcccgct tattacgctt ggtatactac ctaatggttt
     7021 agttgtaaac cataattcaa aatactttaa tgatctcaat gccttatata tttcgattat
     7081 tgttagcatc attattttcg cgatagtaac atttttaaat aataagaaaa gttggcgatt
     7141 aaaatgataa ttaacgaatt aaagtcatgt aagttgaaat tttctaagca agcgctcaca
     7201 tttgtaccca ttattgtaac catattgttt atattattta taaattggta tttaaacgta
     7261 aatttatgga atggtcgaca aataagtttg tttacagcga gttttaatgc aattacatcg
     7321 ctattaattt ctataaacgt ctatcaagtt atcaattttg aagaaaatat tggtcacttt
     7381 aatcatattt taggaaaagc taataggcta aattggttaa atgcatcaat
//
LOCUS       DAKTGS010000135         7383 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.135, whole genome shotgun sequence.
ACCESSION   DAKTGS010000135 DAKTGS010000000
VERSION     DAKTGS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.135"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(97..1551)
                     /locus_tag="O0286_002360"
     CDS             complement(97..1551)
                     /locus_tag="O0286_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0170707.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            1755..3272
                     /locus_tag="O0286_002361"
     CDS             1755..3272
                     /locus_tag="O0286_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCY0170708.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            3363..3512
                     /gene="rpmG"
                     /locus_tag="O0286_002362"
     CDS             3363..3512
                     /gene="rpmG"
                     /locus_tag="O0286_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0170709.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            3966..4235
                     /gene="rpsN"
                     /locus_tag="O0286_002363"
     CDS             3966..4235
                     /gene="rpsN"
                     /locus_tag="O0286_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCY0170710.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            4392..5369
                     /gene="guaC"
                     /locus_tag="O0286_002364"
     CDS             4392..5369
                     /gene="guaC"
                     /locus_tag="O0286_002364"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCY0170711.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            5391..6407
                     /locus_tag="O0286_002365"
     CDS             5391..6407
                     /locus_tag="O0286_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0170712.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(6787..>7383)
                     /gene="lexA"
                     /locus_tag="O0286_002366"
     CDS             complement(6787..>7383)
                     /gene="lexA"
                     /locus_tag="O0286_002366"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCY0170713.1"
                     /translation="EIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVHGHLSRLEEK
                     GYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVENIEEYFPLPE
                     HLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMTEEDEATVKR
                     FYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
BASE COUNT         2709 a         1055 c         1311 g         2308 t
ORIGIN      
        1 atactagaaa aagatattaa agtatatctg ctttacacca ttaaaaagcg gcaaatgcta
       61 tagataatct aaagcatctg ccgtatgaga atttatttat ttttgattgt cataatcgtg
      121 tggttttaaa ttaattgttt ctagctttac aaattttgtt ttgtgaatga ttttatgaat
      181 aaagtaaatc aacgctagaa tgattaaagg taaaaagttt ttaaaagcat ttaaccattg
      241 atcttttaaa atatattcaa ctgagccacc aaatagcaag aataatagtg tagtgatgac
      301 aatgattggt cctaatggat aaaaaggtgc tttatatggt aggaccttat tagggtcttg
      361 accttgtttt ttaatagctt gtcgcaatcg tatttgtgac caaatgcttg atccccaaac
      421 aactataatc attgaaccaa taatttcaag taaattaaaa acggcatttg aattaaagtt
      481 tgcataaata ataacaataa caacgactgc ataagtagtt aataatgctc ttaaaggtaa
      541 cttagttgtc ttgtttaatt tacttaaaaa ttggggtgct tttttgtctg aacttaagga
      601 atacaacatt ctgcctgttg tataaacacc tgaatttgca gcggataata atgaagttaa
      661 aataaccgcg ttgattactg atgctgcaaa ggctatgcct actctatcga atacaattgt
      721 aaatgggctt tgacttattg aactacttgc tcttaataat gatggatctg tgtacggaat
      781 aattgcacca attactgcaa ttgataagac atagaataaa agaatacgcc aaaatacttg
      841 tttaattgcc ttaggcatag actttttagg gtcatctgat tcaccagcag ttactgctac
      901 tacttctgta ccaccaaccg aaaatccggc gactaataat acgcctaaga aaccagagat
      961 accaccaaca aacggtgctt ggccttttgt atagttttca aatccatatg tatgaccacc
     1021 taagataccg aaaatcatta aaaagccaaa aataacgaat acgataattg ttaacacttt
     1081 aatcaatgat aaccaaaact cagtttctcc aaatgatttt acagaaaaaa tgtttaataa
     1141 tagtaaaatt gtaataaaga ttaagctcca agtaatgggg tggaaaaatt taaatgtgtc
     1201 ccagaaataa agcacatttg acgctactat gacatcaaca cttgtaacta atgaccacaa
     1261 tgcccaatac aaccatccca tggtaaagcc aagagatgag tcaataaagc gtgttgaata
     1321 agagctgaat gaacctgata ctggataaaa tgttgccaac tctccaattg atgacattaa
     1381 gaaatatagc atgacaccaa taacaagata agcgagtata gcgcctccag gaccagcttg
     1441 agaaatgata ttaccagtag ctacaaatag accagtccca attgcaccac ctatagcaat
     1501 catggaaatg tgtcttgagt taagactacg gttcatttta ttatcttcca tatttagtct
     1561 cccatctatt taaatatacc cattattgta agctttttaa gtgtactatt caataactat
     1621 ttagtactgt aaagcgaaaa aattaaaatt ttctgatttt ttaatcatct tgagcatgtt
     1681 taattgtaat tttgatgggg ttaaattata atatgtatta aattataatt attataaatt
     1741 gtggagggat gactatgtca caacaagaca aaaagttaac tggtgttttt gggcatccag
     1801 tatcagaccg agaaaatagt atgacagcag ggcctagggg acctctttta atgcaagata
     1861 tttacttttt agagcaaatg tctcaatttg atagagaagt aataccagaa cgtcgaatgc
     1921 atgccaaagg ttctggtgca tttgggacat ttactgtaac taaagatata acaaaatata
     1981 cgaatgctaa aatattctct gaaataggta agcaaaccga aatgtttgcc cgtttctcta
     2041 ctgtagcagg agaacgtggt gctgctgatg cggagcgtga cattcgagga tttgcgttaa
     2101 agttctacac tgaagaaggg aactgggatt tagtagggaa taacacacca gtattcttct
     2161 ttagagatcc aaagttattt gttagtttaa atcgtgcggt gaaacgagat cctagaacaa
     2221 atatgagaga tgcacaaaat aactgggatt tctggacggg tcttccagaa gcattgcacc
     2281 aagtaacgat cttaatgtca gatagaggga ttcctaaaga tttacgtcat atgcatgggt
     2341 tcggttctca cacatactct atgtataatg attctggtga acgtgtttgg gttaaattcc
     2401 attttagaac gcaacaaggt attgaaaact taactgatga agaagctgct gaaattatag
     2461 ctacagatcg tgattcatct caacgcgatt tattcgaagc cattgaaaaa ggtgattatc
     2521 caaaatggac aatgtatatt caagtaatga ctgaggaaca agctaaaaac cataaagata
     2581 atccatttga tttaacaaaa gtatggtatc acgatgagta tcctctaatt gaagttggag
     2641 agtttgaatt aaatagaaat ccagataatt actttatgga tgttgaacaa gctgcgtttg
     2701 caccaactaa tattattcca ggattagatt tttctccaga caaaatgctg caagggcgtt
     2761 tattctcata tggcgatgcg caaagatatc gattaggagt taatcattgg cagattcctg
     2821 taaaccaacc taaaggtgtt ggtattgaaa atatttgtcc ttttagtaga gatggtcaaa
     2881 tgcgcgtagt tgacaataac caaggtggag gaacacatta ttatccaaat aaccatggta
     2941 aatttgattc tcaacctgaa tataaaaagc caccattccc aactgatgga tacggctatg
     3001 aatataatca acgtcaagat gatgataatt attttgaaca accaggtaaa ttgtttagat
     3061 tacaatcaga ggacgctaaa gaaagaattt ttacaaatac agcaaatgca atggaaggcg
     3121 taacggatga tgttaaacga cgtcatattc gtcattgtta caaagctgac ccagaatatg
     3181 gtaaaggtgt tgcaaaagca ttaggtattg atataaattc tattgatctt gaaactgaaa
     3241 atgatgaaac atacgaaaac tttgaaaaat aaatttgata tgtagtttct atattgcgta
     3301 gttgagcagt ttatgatatc ataataaatc gtaaagattc ctaacaagag agggtgttta
     3361 acgtgcgcgt aaacgtaaca ttagcatgca cagaatgtgg cgatcgtaac tatatcacta
     3421 ctaaaaataa acgtaataat cctgagcgta ttgaaatgaa aaaatattgc ccaagattaa
     3481 acaaatatac gttacatcgt gaaactaagt aattcttatc attcaaatac gacgatttga
     3541 aaataaagcg ggcttaccta ttatattggg gagctcgctt ttttatgaaa tttttgtgaa
     3601 gagtgattaa tggattgagt ttcatcggta gaacaatata tgattatatt agttgttact
     3661 ttattaaaat ttgagaatat ttatagaagg aaatagatta ctgattttat aaagtcactt
     3721 tgttagcgaa tgcttgaaag agtatttaat atagtagaat ttaaaatttc aaagcggaat
     3781 ttaataagta cgaagtaatt ctgggtatgt tttataaatg ttcgataata cactttaatc
     3841 ttaaatatga tggtttagaa aatgatttaa caaagaaatg aaactttact gttgaattat
     3901 gtgaggattg tgttattata taaatcgtaa taattacgat ttgataaaaa gtgaggtaac
     3961 tatatatggc taagaaatct aaaatagcaa aagagagaaa aagagaagag ttagtaaata
     4021 aatattacga attacgtaaa gagttaaaag caaaaggtga ttacgaagcg ttaagaaaat
     4081 taccaagaga ttcatcacct acacgtttaa ctagaagatg taaagtaact ggaagaccta
     4141 gaggtgtatt acgtaaattt gaaatgtctc gtattgcgtt tagagaacat gcgcacaaag
     4201 gacaaattcc aggtgttaaa aaatcaagtt ggtaaaatca aatttcgtac tttagcccat
     4261 ttacaatatc aataaaacaa tgtacagtat atacgaatgc tataaactga atgttttctc
     4321 atattaataa ggaaacattc ggtttttaat ttgcatttaa aaataattag tttttaaagg
     4381 ggctatttaa agtgaaaata tttgattacg aagatattca attaatacct aataaatgca
     4441 tagttgaaag taggtctgaa tgtgatacaa ctatccaatt tggtccgaaa aaattcaagc
     4501 tacctgtagt tcctgcaaat atgcaaacag ttatgaatga gaaattagcg aaatggtttg
     4561 ctgaaaatga ttacttttat atcatgcatc gttttgatga agaagcaaga atacctttta
     4621 taaaacatat gcaaaattca ggcttatttg catctatttc agttggtgta aagaaagcgg
     4681 aatttgattt tattgaaaag ttagctcaag aaaaattaat ccccgaatat attacaatag
     4741 atattgcgca tggtcactca gattcagtga taaacatgat taaacatata aaaacccata
     4801 tacctgatag ttttgttatt gctggtaatg ttggtacgcc agaaggtgtt agagaattag
     4861 aaaatgctgg tgctgatgct accaaagtcg gtataggtcc tggtagagtt tgtattacaa
     4921 agattaaaac aggttttggt actggtggtt ggcagttagc ggcattaaac atatgtagta
     4981 aagcagctcg taaacctttg attgccgatg gtggtataag aacgcatggc gacattgcta
     5041 aatcaattag atttggtgca tcaatggtca tgattggttc attatttgcg gcacacgaag
     5101 aatcacctgg tgaaactgta gaacttgatg gtaaacagta taaagaatat tttggtagtg
     5161 catctgaatt tcaaaaaggc gaacataaaa atgtagaagg taaaaaaatg tttgtagaac
     5221 ataagggttc attaatggat accttaaaag aaatgcaaca agatttacaa agctcaattt
     5281 catatgccgg tggaaaagac ttgaaatcat tacgtactgt agattatgtt attgttagaa
     5341 actctatttt caacggtgat agagattaat atttatagta ggtgatgtaa attaaaaaat
     5401 tcatagtaac tgttgttgcg tttttatcaa ttattatcat tgcgccaata acagaattta
     5461 aaccattcat tcatttacaa aatgaagtaa gacaatatat tgacattcac atcaataaag
     5521 aaacaatttc tgcggaaaat aaattggata caccgaagaa acaacaattt gcctttaata
     5581 atatacaaat gaacatgtcg aaatcagatg ttgagaaaac attaaataaa ccaaaaagag
     5641 tgacatttaa tgaatatggt acgaagtggt atacgtatta tgatgacgat tacaataatt
     5701 ttataatgat aagttacatg aaagataaag ttaatgcgtt atatacaaat caaaatataa
     5761 tcacttcaaa atcaaaaatt aaatataata cacctaaatc ggttgtaagg caaagattag
     5821 gcgaaccaga aacagagatt gttaaaggta gagtgcgtta cgaacaaaat aataaagaat
     5881 atgatgtttt ccataaaaat cacatttata cgacggtatt ttatgataag catcgacgta
     5941 ataatgtaac agctgtttta caagtaagtg atgctatgga aaatagatta aaagaacaat
     6001 atggagcacc atcgaaatcg cttgcagata gttttgaact acaaaatttt gatttagtta
     6061 atgctgaaag aaaacaacat caattatcta cattgaagta ttctaaacag aattctgaaa
     6121 ctgcacgtaa gcatagtaaa gatatggcca acaatcatta ttttgatcat acaaatttaa
     6181 aaggtcaatc accatttgat cgattgaaaa aagatggtat tacatttaac tcagccggag
     6241 agaatttagc atatggtcaa gttagtagta tctatgcaca tcaaggatta atgaattcta
     6301 ttggtcacag aaaaaatatt ttaaatgata cgtttaaaat attaggtgtt ggtgttgatt
     6361 ttaatgatga aaaacaacct ttttggacag aaaattatac tggttaatgt ttttggcatg
     6421 agattaaagg gtaatgtttg tctgtaagga agtagataaa catgatgaca acagataaac
     6481 caaaagatgc ggatatactt gaacgcgtca aagatatact taataaaaaa gaaagaaaaa
     6541 aataataatg gctcgctcct gtaaattatt acgggggcga gttttaattt gtgtaacatt
     6601 ttgcagaaat ctgtaattca aaatttatat taaagaatga gattatctaa caattcgcaa
     6661 tttctgtttg aactgaattt agattagaat aaataaaacc tcttgaaaac gtaacttttt
     6721 caagaggtcc aaaatttata aaaatgaggg cttaacaatt acgataatat ataacggttt
     6781 aaaatattac atttcgcggt acaaaccaat tactttccca attacagcaa cattgtctag
     6841 gtaaattggc tccattgtac tattttcagg ttgtaatcga taacgatttt tttctttata
     6901 gaagcgtttg acagttgctt catcttcctc agtcatagca acaataatgt ctccattttc
     6961 tgctatggtt tgactgcgaa caattacttt gtctccgtct aatataccag cctcaatcat
     7021 actgtcgcct acgacgttta atatgaatat gtcgctattg tgtgtcgatg ttaagtgttc
     7081 aggtaatgga aaatattctt caatattttc tactgcggta ataggaacac ctgctgtgac
     7141 tttaccaata actggcacat gaatcgtttc ttccatatta atattatcat ttgtttgatc
     7201 acttacaatt tctatagcac gtggtttcgt tggatctctt cttatatagc ctttttcttc
     7261 aagacgtgaa aggtgaccat gaacagttga actggatgct aagccaactg cttcaccaat
     7321 ttcgcgaaca ctaggcggat aacctttcgt ttgaacaact tgtttaatat agttatatat
     7381 ttc
//
LOCUS       DAKTGS010000136         7356 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.136, whole genome shotgun sequence.
ACCESSION   DAKTGS010000136 DAKTGS010000000
VERSION     DAKTGS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.136"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..330
                     /locus_tag="O0286_002367"
     CDS             <1..330
                     /locus_tag="O0286_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0170714.1"
                     /translation="SVEFQKFSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRS
                     FEHQIEDEYLLKLKKDYREYYESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPM
                     IGDITNE"
     gene            323..985
                     /locus_tag="O0286_002368"
     CDS             323..985
                     /locus_tag="O0286_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0170715.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(1101..1784)
                     /locus_tag="O0286_002369"
     CDS             complement(1101..1784)
                     /locus_tag="O0286_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0170716.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2034..3110)
                     /locus_tag="O0286_002370"
     CDS             complement(2034..3110)
                     /locus_tag="O0286_002370"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCY0170717.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            complement(3445..4410)
                     /locus_tag="O0286_002371"
     CDS             complement(3445..4410)
                     /locus_tag="O0286_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0170718.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(4624..6261)
                     /locus_tag="O0286_002372"
     CDS             complement(4624..6261)
                     /locus_tag="O0286_002372"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCY0170719.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(6421..7299)
                     /gene="hchA"
                     /locus_tag="O0286_002373"
     CDS             complement(6421..7299)
                     /gene="hchA"
                     /locus_tag="O0286_002373"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCY0170720.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
BASE COUNT         2308 a         1390 c          992 g         2666 t
ORIGIN      
        1 tctgttgaat ttcagaaatt cagtaaaatt tatgatattt taactgaaga tatgattatg
       61 ccgaatatga ttatcttttt agatgcagac cttgatgtgt taaaatctag aattgctaaa
      121 cgtaaccgta gttttgagca tcaaatagaa gatgaatatc tgttaaagtt aaaaaaagat
      181 tatcgtgagt attatgagtc cttacaaagt aatggttcaa atgtagtttt aatcgataca
      241 acttctattg attttcttaa aaatgaacaa gattacgaag atatattaca tattatatta
      301 cctatgatag gagatattac caatgaataa ttacggtatt ccacaaaatg ccattataac
      361 cattgcaggt acagttggtg ttggaaaatc aacactaacg caagcacttg cagataaatt
      421 aaactttaaa acgtcttttg aaaatgtcga acataatcca tatttagata aattttacag
      481 cgattttgaa cgatggagtt tccatttgca aatttacttc ttagctgaac gttttaaaga
      541 acaaaagcgt atgtttgaat atggtggtgg ctttgtccaa gatcgatcaa tttatgaaga
      601 tgttgatatt tttgcaaaaa tgcatgaaga agaaggcaca atgagtaaag aagatttcaa
      661 aacatattca gacttattta atgccatggt catgacacct tattttccta aacctgatgt
      721 aatgatttat ttagaatgta actatgatga ggtcattgat cgtattattg aacgtggtcg
      781 cgaaatggaa attaatacag accctgaata ctggaaaaag ctatttaaac gctatgacga
      841 ttggattaat agctttaatg catgtccagt tgtacgtatc aatattaatg aatatgatat
      901 ccataaggac cccgaatctt taaatcctat gataaacaaa attgctcgaa ttattcaaac
      961 atatcgacaa gtagatacac gataaaagac taaagacata gcgtatatgt ttatattcaa
     1021 tgtatattcc atagatatta tcgattattt tatcaattct atcgaataca ttaattcaca
     1081 tatacactat gtctttcttt ttaatttaaa gcttctaaaa tatctgccgc actatttaaa
     1141 ataatatcag cttcatgtaa ttcttctttt gttgcaatac ctgttaatac acctattgcc
     1201 atacctaaat ttgcattact tgctgtcttc atatcattag cagtgtctcc tactatagct
     1261 actttctgag gatctacatt atattgctca aataaaggcg ataatacttt aggatttggc
     1321 ttctcatagg catccgcttc ggtagaaatg atcaaatcga acaacgaggt agcattggta
     1381 tgtgctaaaa attgttctac acctttttta gtatcactcg taacaatacc aagttgatag
     1441 ccttttgctt tcaaatcgat aagtgcttct ttaacacctt ctacccaatt aatttcagga
     1501 atacgttcat ctaccagctt ttgacttgtt gacttggacc agtcggttgt atcttgtccc
     1561 gtcacatcat taaatgcctg gataatttgt tgtaaagatc ctgaacccat cactgatttt
     1621 ggatcaatag attctttaat gacaccgagt tgtcttaaag cagcttcttt attatgtact
     1681 gggaaagtct caagcaatga ttgtacaaat cgtaccccta ttttttccca acttctatca
     1741 aattcaatta acgtaccatc tttatcaaat aatatccatt ccattgatat caatactcct
     1801 atttatttat ttcgtattat gctgattcta tgatattcgt tatcccctga aaatgaactc
     1861 gtagtattgt tctatttaaa tattgaatta aatataataa taagtgaaat ccccttcaat
     1921 acttaacaat aaacattgta aacttaattt attaccatgc ttcgcttcat tgaaagggat
     1981 tttagtcatg attaactttt gcatattgtt ttcatgatta tattcaattt ttattaatat
     2041 tttggtacaa cgactctcca accattttta tcttctaaag taccattttg aataccagta
     2101 tagacgtcgt ataatttttg agtaatttca ccagtctcat tattattaat aacgatttca
     2161 cgatcttcgt atctcaatgt acccacaggt gaaataactg ctgcagtacc actaccaaat
     2221 acttctgtta actcaccttt atcatatgat tcgaataatt catcgattga aacgcggcgc
     2281 tcttcgactt catatcctaa gtttttagct aattcgataa tagatttacg tgtaatacca
     2341 ggtaaaatac tgccattcaa ctctggtgta attactttgc cattttcaac gaagaaaatg
     2401 ttcatgctac caacttcttc gatatatttc tgttcaacac catcaagcca taatacttgg
     2461 tcataaccta atttatttgc attagtttgt gctaataaac ttgccgcata gttacctgca
     2521 acttttgcaa agcctacacc gccacgaaca gcacgcacat attcatcttc tacatagatt
     2581 ttagttggtt ttaaagtttc accaccataa tatgcacctg aaggagataa aataattaat
     2641 aatttatact gatgtgatgc accaacgcca agtgcccctt ctgttgcaaa aacaaatgga
     2701 cgaatatata atgattgacc ttccccttca ggaatccaat ctctttcaat atcaactaat
     2761 tgttttagcc cctctaacaa ttctgcttcg tctacttgag gcatttctaa tcgtgctaac
     2821 gagttattaa gacgcttaaa attttcttca ggacggaaaa gtgcaacttc cccatctctt
     2881 ttatatgctt ttaatccttc gaataccgat tgaccataat gaacaccttg tgcagcaggt
     2941 gaaatttcaa taggaccata aggtactatc ttcaaatcat gccatccttt atctgcatca
     3001 taatcataac tcaacatata atcagtaaaa tatttaccaa aacctagttg agatgtattt
     3061 ggtttttgtt ttaatgtttc tcgtcgttca actttaactg cttgtgacat ggtgattgcc
     3121 tcctaataat attgtataag aatttgttta acttaaatta taacaatcca tattttgctg
     3181 ttcaacaaat tttctaaaaa ttcaaaatta attaacagat ttctagaaag actatatctt
     3241 ttagtataaa cgtattaatt tcacagagac aagtaatctg tgttttacta atatacttta
     3301 catacaaaaa actctttact ttaaaatgaa ctaagctcgc gaattcaata agtataatga
     3361 ataatattag aattcatgca ctagtttatt aaaataaaga gtaatttaaa atatcattcc
     3421 gtgtattaaa gtgaatggaa atgattagtt attattttta acagtatctt tttgttcaat
     3481 agcttctaac attaatttag tcatgctcgc taaatcatat ttaggatcaa atccccattc
     3541 gccacgtgaa cagcttgtat caatagaatc cggccaacta ttagcgatac cttgtctaat
     3601 aggatcaaca tcgtaatcta atgtaaaatt gggatagtat tcttgaattg cttcttttac
     3661 catctctgga tcaaaactca ttgcgctcaa attataacca tttctagttt ctaatttagc
     3721 gtcgtctgct tccataagtt taataattgc ttcaattgca tcatccatat acatcatatc
     3781 catatacgtg cctttatcta tgaagcttgt ataatgaccc tctcttactg ctttgaagta
     3841 tatttcaaca gcatagtctg tagtaccgcc acctggctct ttaacatgcg agattaaacc
     3901 tgggaatcta acacttcttg tatctacacc aaaacgtttg aaatagtatt gacacaataa
     3961 ttctccagct actttattta caccatacat tgtcgtaggt tgctgaatcg ttacttgtgg
     4021 cgtattaact ttaggagttg agtctccaaa tgcaccaatt gaacttggtg tgaaaaagtg
     4081 caaattataa gttcttgcag cttctaatgc attcattaat ccacccatat ttaaatccca
     4141 agctagaatt ggatttttct cagcagttgc tgataataat gctgccatat gcattagact
     4201 atccgcttca aagtccctaa ctaactcaaa catacggtca cgatctgtta cgtctaagat
     4261 ttcaaatggt ccattttgta caggtgagtc tgcttcaggt tccctaatat ctgtagcaag
     4321 aacattatct gtcccataaa tttctctgca cttaacaact aattctgtac caatttgtcc
     4381 taatgcacca gtaatcataa tttttttcat agaaatatct cctttgtctc ttctatatag
     4441 ctatagtcca tcacaagcgg acataatatt cattttcata ataattataa tataaaagcg
     4501 ctttcttgta tatatgacat gtacatgttg ctgatatttc tgtaaatgga aattctagtt
     4561 gtattaattg attttagtaa tttatagcgt ttattattgc taattactgt caaattaaat
     4621 tttttatccc tcaactctta aactctggat atctttcatt atattagctt ttttataacc
     4681 atggatatca tgtaaagcct tataagcttt aaataatgtt tcataccttt gtactttttc
     4741 cgcttctgga ttaatttcgt ataaaattgg ctctttcata gcatcaactg cgtcattaat
     4801 tgaattatgt gcgccaccac tgactgcacc taatattgct gcgcctattg ctggtgcata
     4861 ctcactatcc ataacaatta gttttttatt cagtacattc gcatagatat ccatcataac
     4921 agcattcttt ttaggtatac cgccacatgc aaataccttt tctacttcca tatgccaatc
     4981 ttgatactgt tgcataatca tcttagtacc aaatgctgta gcttctaaat atgctctata
     5041 aatatcctca tgcttagttt gtaaagttaa tccaaagata caacctgtta aattgctatc
     5101 acttaataca cttcgatttc cattatgcca atcaagagca atgagcccac tttcacctgg
     5161 catttgatgt tttatctttt cattcattaa ttcaaataca gtcatatttc tattttctgc
     5221 ttcatctaca tatgactttg gtgcttgctt agcgacatac tcaaacaaat cacctactgc
     5281 tgattgcccc gcttcataag caaataattc tggaataatt gctcctttta cagaacctga
     5341 tatacctggc acttgatgtt gcttttcatt taacataaga tggcatgtgc ttgttcccat
     5401 caccatagtc atttctttat ctttttcaga cccaatacct aataaactag catgggcatc
     5461 aataataaaa ggacttacca tagtttcttt tgataatcct aatttctgtg ccattttatc
     5521 atccagtttc cctactgctt caccaatatt aacaaccggt gcagatactt tatcttgaat
     5581 tacttttgat aatttggggt ctattttatc aaataaatca taatgaaacc ctgtttcttc
     5641 ttcccaaaat gctttgaaac ctaatccaca attcgagcgt acatttttat tagttaattt
     5701 atttacaatc caatcgcccg cttccataat ataagccgtt ttttccataa tttctggtgc
     5761 tcgattcatg acctccatta ttttgggaat catccattca ctactaacat tatatccata
     5821 atggcctaac cacttattat tattttcaat agcagtttga tataatttct ctgcctcttt
     5881 atatgcacca tgatgtttcc aaagtttcac atacgcatgt ggattgtttt taaattgttt
     5941 taagttatgt accgggttaa ggttttcgtc agtaaaaata atagtagatg aagtaaagtc
     6001 tataccaata cctactatat tgtctggatc tatttttgat tcacgtacta tatatgatat
     6061 tccttcttcc ataatttcta aataatcatt actattttga agtgcatatg tatgtggtat
     6121 tttcaaacca tttaattcac tctcaatgac accatgtgta tatggtttca caaattttga
     6181 tactacttga ccgttagttg tattaattaa aaacacacgg cctgaagcag ttccataatc
     6241 aattccaatg ctataagaca tcatgtttcc tccatatcgc tagttgcggt tattagtttt
     6301 gcaagcgttt tcttaaatca tactcataat aggttctatt ttacaagtat cacttaaatt
     6361 ttgccaaaaa aaaagagccc tcatacattt acatatgagg ctcctctaat taatatttaa
     6421 ttatttattt tgtattgcat ttaacatttc attaactgct aattttccta actcatttga
     6481 agctaaagga ctgtcacctg ttaataattt acgatctttt aacgttcttc ctgtcatatc
     6541 gtcgttaact acttttaatc cttgtttagt taataaatca gcaactaacc atttcaagcg
     6601 tccaggtaaa taacctattt caatatttgc accttcatct aatgagtcag ggaagacaca
     6661 aacagagtat ccttctaatg gagatttttc tctgttaagc cctgctgaaa gtagtgctgc
     6721 tggtccatga cataatgtaa ctataaagcg gtcattgtct aatgcccaat ctaatgtttg
     6781 ttgaacgtcc tcactttcag aaataccaac aactgcagca tgtccgcctg ggataaagac
     6841 agataaatag tctgaatcag gtcctaattc atttttaatc acatctgcta atttttttgg
     6901 ctgttttaat ttttctttca atttattata agtacttata actgcctcgt cttcagttgg
     6961 catagcccat aattctaatt taactggata accagataat gtcgcaacat caacgtcaaa
     7021 acctgcttcc attaaatgat gtaaaggtaa taacatttca acaggatgat tacccgtaga
     7081 gaacattttt ccattttcca ataatacata tcgctcttca gcagcaatca tcaatacttt
     7141 ccatttacca tctttatagg cacctttgtg ttcaacacca tcaaaatctg ttttaggtgc
     7201 tgtatattga ctaagggaat atggtgatgg gaaaaatgcg ttatcttctg ctttatctgg
     7261 cgttggttgc ttacttaatt catttacatc ttgtgacata gtttattcct actttctcta
     7321 tatagttact actagtatag acatatacta cacact
//
LOCUS       DAKTGS010000137         7336 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.137, whole genome shotgun sequence.
ACCESSION   DAKTGS010000137 DAKTGS010000000
VERSION     DAKTGS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.137"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(149..399)
                     /locus_tag="O0286_002374"
                     /pseudo
     CDS             complement(149..399)
                     /locus_tag="O0286_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            785..2539
                     /gene="eap"
                     /locus_tag="O0286_002375"
                     /gene_synonym="map"
     CDS             785..2539
                     /gene="eap"
                     /locus_tag="O0286_002375"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCY0170721.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSIN
                     LNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKN
                     GGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSD
                     LKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSN
                     ISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISY
                     KYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKD
                     IKKIDIDVKQYTKSKKNK"
     gene            2526..2840
                     /locus_tag="O0286_002376"
                     /pseudo
     CDS             2526..2840
                     /locus_tag="O0286_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
     gene            complement(3164..4450)
                     /locus_tag="O0286_002377"
     CDS             complement(3164..4450)
                     /locus_tag="O0286_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0170722.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            4650..4748
                     /locus_tag="O0286_002378"
     CDS             4650..4748
                     /locus_tag="O0286_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170723.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            4991..5167
                     /locus_tag="O0286_002379"
     CDS             4991..5167
                     /locus_tag="O0286_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170724.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            5431..5811
                     /locus_tag="O0286_002380"
     CDS             5431..5811
                     /locus_tag="O0286_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0170725.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            5808..6704
                     /gene="pmtA"
                     /locus_tag="O0286_002381"
     CDS             5808..6704
                     /gene="pmtA"
                     /locus_tag="O0286_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCY0170726.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            6705..>7336
                     /gene="pmtB"
                     /locus_tag="O0286_002382"
     CDS             6705..>7336
                     /gene="pmtB"
                     /locus_tag="O0286_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCY0170727.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYF"
BASE COUNT         2708 a          954 c         1173 g         2501 t
ORIGIN      
        1 agatttttca gtctagcttt tggggtgtac attccacaca aacatgtgat tattttgatg
       61 tttctattaa acttgtaatt ttaaatttaa agtccctaaa aagtccctaa aattttattt
      121 tatatgaggt attattgata atgataaagt tataaacctt gatattatgc tgttttactt
      181 tttgaatgat aagtaatttt atgttaaaag tctccagttt ggatacaaaa cggtcgataa
      241 catataaacg ttatgactaa ctaacttcaa atcagtatca tctttcttag attcggcttt
      301 ggcactattg tcagtaagtg caccaactaa taataaattt gctaatgcaa gtgttgcaac
      361 tttttttagt gaattggatt ttgttttttt caccatcatt atcactcctt ttatatagct
      421 tacaacaaaa tagatgcaaa attgattaac taaatttaaa ttaattatta aatcgaattt
      481 atatttttaa gtaaatgttt tttgagtata aagatgctgt aaatataatc gacaaatcta
      541 atcggaatta agaaatgaat gttgttatga catttaagtt tgaagcttgg tataaacaaa
      601 accaaacaac attttaggta ttgtaaatta attagttaat caaaattaaa agaactttta
      661 acagtgttaa aaaattattg ttaaagataa tttattatta atattcagtt aattcaaaaa
      721 atagagaaag tctggctata attaagttgc aaatacgaat tacacaaaaa aggagagata
      781 atttatgaaa tttaagtcat tgattacaac aactttagca ttaggtgttt tagcatcaac
      841 aggtgcaaac tttaataata atgaagcgtc tgccgcagct aagccattag ataaatcatc
      901 aagttcgtta caccatggat attctaaagt ccatgttcca tatgcaatca ctgtgaacgg
      961 tacaagccaa aatattttat caagcttaac atttaataag aatcaaaata ttagttataa
     1021 agatttagag gatagagtta aatcagtttt aaaatcagac agaggtatta gtgatataga
     1081 tttaagacta tcgaagcaag cgaaatatac tgtttacttt aaaaatggaa caaagaaagt
     1141 tatcgatttg aaagcaggta tttacacagc tgatttaatt aatacaagtg aaattaaagc
     1201 tattaatatt aacgtagata ctaaaaagca agttgaagat aaaaagaaag ataaagcaaa
     1261 ttaccaagtt ccatacacaa tcactgtgaa cggtacaagc caaaatattt tatcaaactt
     1321 aacatttaat aagaatcaaa atattagtta caaagattta gaggataaag ttaaatcagt
     1381 tttagaatca aatagaggta ttaccgatgt tgatttaaga ttatcgaagc aagcgaaata
     1441 tacagttaat tttaaaaatg gaacgaagaa agttatcgat ttgaaatcag gtatttacac
     1501 agcgaattta ataaattcaa gtgatattaa aagtatcaat attaacgtag atacaaaaaa
     1561 acatatcgaa aataaagcta aaagaaacta tcaagttcca tattcaatta atttaaatgg
     1621 tacatctaca aacattttat cgaatctttc attttcaaat aaaccttgga caaattacaa
     1681 aaatttaact agtcaaataa aatcagtact gaagcatgat agaggtatta gtgaacaaga
     1741 tttaaaatat gctaagaaag cttattatac tgtttatttt aaaaatggtg gtaaaagaat
     1801 cttacagttg aattcaaaaa attacacagc aaacttagtt catgcgaaag atgttaagag
     1861 aattgaaatt actgttaaaa caggaactaa agcgaaagca gacagatatg taccatacac
     1921 aattgcagta aatggcacat caacaccaat tttatcagat ttaaaattta caggtgaccc
     1981 acgtgtaggc tacaaagata tctctaaaaa agttaaatca gtattgaagc atgatagagg
     2041 tatcggggaa cgtgaattaa aatatgcaaa aaaagcaact tacacagtac attttaaaaa
     2101 tggaacgaaa aaagtgatta acataaattc aaatattagc caactgaatc tgctttatgt
     2161 tcaagatatt aaaaagatag atattgatgt taaaacagga actaaagcga aagcggatag
     2221 ctatgtacca tatacaattg cagtaaatgg cacatcaaca ccaattttat caaaacttaa
     2281 aatttcgaat aaacaattaa ttagttacaa atatttaaat gacaaagtga aatctgtatt
     2341 aaaaagtgaa agaggcatca gtgatcttga cttaaaattt gcgaaacaag caaaatatac
     2401 agtatatttc aaaaatggaa agaaacaagt agtgaattta aaatcagaca tctttacacc
     2461 taatttattt agtgccaaag atattaaaaa gattgatatt gatgtaaaac aatacactaa
     2521 atcaaaaaaa aataaataaa tctaataatg tgaaattccc agtaacaata aataaatttg
     2581 aaaacatagt ttcaaatgaa tttgtgttct ataatgcaag caaaattaca attaatgatt
     2641 taagtataaa acttaaatca gcaatggcaa atgatcaagg gataactaaa catgacatag
     2701 gacttgctga acgcgcagtg tataaagtgt attttaaaaa tggttcgtca aaatatgtag
     2761 acttaaaaac tgagtataaa gatgaaagag tatttaaagc aactgacatt aaaaaggtag
     2821 atattgaact taaattctaa tctttaattt tataataagg catctcacaa tcgtggggtg
     2881 ccttttacat ttgtagagat gtgatgcttg aatgtttgaa gtgatttgcc aaaagttata
     2941 taaattggtt ctataataaa tcggactgat agaaaaagtt ttttactttt catctgtccg
     3001 attttttgat tttgttagga gagatgtata aaactaatta ggtatagtgt ttgacaagtt
     3061 gctgaataaa atatatctta tgatactttg aaagaaggaa taattttaaa aataaaaaac
     3121 caataatccg agtcatactc atcagattat tggttgaaat taattatctt aagtcatcaa
     3181 tcgctttagc aattgaatca aatacatgtg ggatatcttc tttttctaca cagctaaacg
     3241 caatacgaat atctgttgca ttaagtgcaa taacaccaat tgaatatttt tcaattaaat
     3301 gtttacgaag ttcttcaggg tcaacatcat gaacttttat tgccataaag tatccagagt
     3361 taaagtcata ggcttgccaa tgtgaatgat attgatcagc atagacaact tccttagtga
     3421 cttcatagcg ttcttttaat gtttgaatat tttgttcgat ttctttatcg aattgtttat
     3481 tattttttaa tacatgctta acagcgcttt gtgttggaag aggtccacta gaaatgttac
     3541 ttcgtataag accttttact ttggcttcta atacttcttt agttgtttga tcagacgttc
     3601 caaatgtcat aaacccaaca cggaatcccc atgcgaaaaa ttcttttgtt gcaccatcta
     3661 aacgaacagg taatattgca tttgaattta aattagataa tgcagtaaat aatgattgag
     3721 tatacacatc ttcatagaat aaaccatagt atgcgtcatc cacaacagct ataacttttg
     3781 tacctttatc agctaatgct ttaattgcat cgacgatggt agtcacttct ttatgcgtag
     3841 gtgtgtaacc tgtcggatta ttaggataat ttaaaatcat aatgacttta tctttattgt
     3901 atgattgtaa agcttctaca agtgaatcag tagtataatg cccgtcttta tcaaagatag
     3961 gatatgtttg aaggtttgca ccatttctcg tattgaaaac aagtttgtaa ttaccccaat
     4021 tatgctctgg taacaagata gtgtcacctt gatttacaaa taaatcgcca actaaagata
     4081 aaccatgtgt taatgcattc gtaacaattg gtagtgacat gttgtcgatt gatagctctg
     4141 gattgtcacg caacattttt tgttgccata aatcacgtaa ttcttcgatg ccttgtggtg
     4201 gcgcataagg gaatatttca tctggagtta aatcattaaa cattgcatct aaagatgatg
     4261 caaacatttt tccgtctttg tttgtcgcca tacctatagt tgcattatat gttgtactct
     4321 ttgcttcagc agattgagat aaaatgcctt ttggataaaa catattttga cctaagtcag
     4381 ataacattgt aaaggcagtt gcatttgact gttgaagttg ttcatttaag ctttgggcta
     4441 aaggattcat tattgcttaa cctctcttta aaaaaattgt atgcctatat attatcttca
     4501 attataccaa agataaagtg ttgttggtga attaacgaat aatatgtaaa atctgctgcc
     4561 aaaatacgaa catatgttct gtttttatgt tagaataggt gtaggtcatc cttcagtatg
     4621 atactttagt cgaacggagg tgtagtgtaa tgatgtggtg gcaagaaggg atgatgacac
     4681 ttattacagg tggcttacta attatttttc gactttggtt agaactgaaa tggaaaaata
     4741 aaaaatgatc ctacacacat aaataacccc aagtctgctg gtaccagact tggggttgtg
     4801 tagtataatt ctgtggatac acttaaataa tatcattgaa cacaatatat cgcaatcaat
     4861 aatcattttg attgaatggt tttgttgcgc tattttagtt gaaagtgatt agtaccttga
     4921 acttttgaaa ttcaataact tattaaaaaa atttatattt gttataatgt cgatgttgag
     4981 gtgaaataaa ttgaaacatc taacaaaaat atttgtagta cttgcaatta ttttatttat
     5041 tattggttat tatttacaag caactaatca tgaaagccaa ggtataaaat tattattagc
     5101 agcgattatg tttatgattt gtgcttttat aagtagaagt aatgatcgac gtaaaaatag
     5161 taaatagaat tgactgttta aaacgattgt ttatgttgac gctacggttg tcacgtagag
     5221 attaaagaca ttcgttttag cagtcttttt tattgaagta gacaatgcaa gattaaatgg
     5281 tagtgtcatt tcattttatt ttattcaact gctagtaata caatactcag tgttatttct
     5341 gaagactggt tgataatgag acatgcatat ttacaaaact gtgtatattg tgtatattgt
     5401 atatatacag aagttgatag ggggacgttg atgaaaataa ttttaaaaaa caatagtgat
     5461 tttccgattt atgaacagat taagcaacaa gtaaaacaaa atattttaaa gggacatgtt
     5521 gctcctggag agcatttgcc gtcaatgaga gaacttgcca aagatcttca agtaagtttg
     5581 attactacca aacgtgctta tgaagattta gagaaagacg gttttgttac aacaattaga
     5641 ggaaaaggga cctttgttaa ggagcaagat agttctattt taaaagagaa acaatttttt
     5701 accattgaaa atttggttaa agaattggtt aatgaagcgc aagccatcga aatgtcactt
     5761 gaggaactgc aggatatttt aacgttcatt tatgaggagg aatcatcatg aatgccatag
     5821 aattaagtaa tgttaattat tcaagtgatc agtttaattt aaagaatatc tctttcaaag
     5881 ttcctcaagg attcgtcact ggttttattg gtagaaatgg cgctggtaaa accacaataa
     5941 taaggttaat tatggatttg tatcaaccac aaactggtgt tattagagta ttagaagagg
     6001 atatggctct caatccgata gagttaaaga atagaattgg gtttgtttac tctgaaaatt
     6061 attttaatga gagatggact acgaagcaac ttgaaaaaat gattgcccct ttttatcgta
     6121 aatgggacca tcaagtattt gaattttatc ttgaaaagtt cgatttacca atcaataaat
     6181 caataaaaac attttcgact gggatgaaaa tgaaattatc actagcagtt gcattttcac
     6241 atcatgctga attgtatatt tttgatgagc ctacttcagg tcttgatccc ttagctagaa
     6301 atgagctatt ggagataatt cagcaagaat taattgatga aaataaaaca atattcatgt
     6361 cgacacacat tatctcagat ttagaaaaaa tagctgatta tatcattcac ttaagtgacg
     6421 gtgaagttat cttaaatggt tcaaaagaac aactgttaca aagatatcaa gttgtgagtg
     6481 gtgctattga ggaccttgat gatgaattag catctctact tatttatgaa gaacataagc
     6541 gtacgggatt tataggatta acagaacatg cgcaagtatt taaagaaatt ttaggtcata
     6601 aagtgaatat cactacacct tctattgaga acttaatggt ttatcttgaa aagagaaaac
     6661 ctaaatatca tgaaaacatc aaattgatgg aagaaggttt ttaaatgaag gcattgttaa
     6721 ttaggaattt taaattacga cgatatacac ttatcatcta tgttctatta ttaaccttat
     6781 atccttttta tataatgctt gactcaacaa agttctttta cttattgcaa tcttttataa
     6841 gtccaacaat attgattatt tggattttag atgctggtca cttatttaga ctcaatcgta
     6901 gattaggtgg taatgattca tattattttt atatgagttt accagtgtca aaaaaacaat
     6961 tgcttaatgc caactatatt acatgcattg ttttaacatt aattggaacg cttgttatta
     7021 gtttatatgc ttatgaagca gatgtgattg aaccaaattc aatctatttt tcaactgcat
     7081 atgcatttgt catatctaac ttcttgtcta taccaattgc atttagtcaa tttacagaat
     7141 tgcgtagagt caaagtgcca tatggtatat acgtgtttac tattatcatt ttagttccat
     7201 ttttattttc aattgcaata gtattggtga attattttgt tctaagtcaa tcatcattcc
     7261 cagatttata ttcatatatt ttaaatattg gttttctaat tataagcatt gttatactta
     7321 ttgttaatta ttttaa
//
LOCUS       DAKTGS010000138         7315 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.138, whole genome shotgun sequence.
ACCESSION   DAKTGS010000138 DAKTGS010000000
VERSION     DAKTGS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.138"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..566)
                     /locus_tag="O0286_002383"
     CDS             complement(<1..566)
                     /locus_tag="O0286_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0170728.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
     gene            644..745
                     /locus_tag="O0286_002384"
     CDS             644..745
                     /locus_tag="O0286_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170729.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            complement(830..1513)
                     /locus_tag="O0286_002385"
     CDS             complement(830..1513)
                     /locus_tag="O0286_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCY0170730.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(1879..2577)
                     /locus_tag="O0286_002386"
     CDS             complement(1879..2577)
                     /locus_tag="O0286_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCY0170731.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(2957..3655)
                     /locus_tag="O0286_002387"
     CDS             complement(2957..3655)
                     /locus_tag="O0286_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCY0170732.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(3999..4694)
                     /locus_tag="O0286_002388"
     CDS             complement(3999..4694)
                     /locus_tag="O0286_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCY0170733.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(5115..5810)
                     /locus_tag="O0286_002389"
     CDS             complement(5115..5810)
                     /locus_tag="O0286_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCY0170734.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            complement(6256..6960)
                     /locus_tag="O0286_002390"
     CDS             complement(6256..6960)
                     /locus_tag="O0286_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCY0170735.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
BASE COUNT         2190 a         1337 c          865 g         2923 t
ORIGIN      
        1 attaggaatt catatgcatc acctaacata tcaatttcca tgtcactgtg aacgaatggt
       61 aagtcgtcaa gattaaccat gactttagag attaaagcag tacgttcttt gacattgtta
      121 cctagtcgcg ttgaactcaa atccatatcg ctgaacagac cgataaagtc attttcactt
      181 tcttcaccta atgttgatgt ttcaacttta cgaattgccg tcgccaggtg ttcgatatcg
      241 aaatcttgcg tttcaatttc acgaatcatc gcactgaata aatcttctgg ctcaatgaag
      301 taaccgactt ggtcaattaa ttctgctttt aagtcttcac ggtattcttc gtctgcccat
      361 gcttcttgat acgtgatgtc ttcacctgac aaggcatctg catattcttg ttccgctttt
      421 tcagataaga agcgatagaa aatcaagcct aaaatgtaat tacggaattc actcgcatcc
      481 atattccctc ttaaatcatt cgcaatcgac cataattttt tatgtaattc agcttgttgc
      541 tgacgttgtt tttcagtaat agacatgtga ttcctccgcc tttgaataag taatttatct
      601 ctttgtgtaa tagatttatt ataacatttg gttatgttgc gatgtggaga attttgatgt
      661 tggtggtgga aattttacct ttatggagat tgggatagga ttcatgacac acaaaccaaa
      721 acggcttaac gacgatatcg attgatgcat cctttttgta taagctatgc gcaccaattt
      781 acatttcacc gttaagccgc ttcaattact tttaattatt cgtaatgatt tactttaagt
      841 taacttcaat atctttaatt tgtttgcttt ctatgacttc ccccatatat ttaaatttta
      901 atttagatct taaatcaagg ttataaaaac tgccatcttt caagctaatt ttgaccctac
      961 catcttttga gattgtttta taaagtccgt atttttcaat tagatgcttt ctcaacttat
     1021 aatccagttc ttttagtgtt attttttctt ttttaatgta ataagggtaa cctttcacaa
     1081 aagcatcttc acccttttct tttgaaatat ttaatattgg tgcactgaca actccagatg
     1141 ttttattatt ctgtattaca ccaccaacag tataaaatat atcgtgctta tctctttttt
     1201 cttgaacaaa gaaaacatct aacccctcat aactacgctg ttgaaattta cttttatcat
     1261 ttccaggcag taaaacttga atcttaatac ctctaaactt aaaacgtaag ttgtttttac
     1321 catgtttcaa agcactaata tttttcattt ccatagtctt tccagtgtag tatcggtata
     1381 atgcttcctt gtcatgttta tttactgact tttgattttg ttttgcatga cctgaatgaa
     1441 cttctgttgt taaagttcct gttgttaaaa ttcctaaagc taatgtcgct tttgctaatg
     1501 ctgtaaattt catatttatt tgctccaatc ttaatatatt ggattgtttt cattacgtaa
     1561 tttgaatcat acatttttat tataacgtgc atcgtttcat acgataatta acctcgattt
     1621 aaatatactt gaattattct taatgaaaaa caaatatatt ttaatgttca ggttaaatga
     1681 ctttcattat ttcatttagt tgttttaccg gattatcgtt tactacaact gatgatgtta
     1741 ctctgttctg ctctttgaat tacaaaaaca acttaacaac agtaatgtat ttaacacagt
     1801 tccttatata aggacacaaa tgccaattca cattttgtcg ttaagccgct tcttaagcta
     1861 ttatgctatt aactcgaact aattcaaatt cacttcaata tttttaattt gcttactatc
     1921 cattacatca aacatacgtt caaaacttaa tttttcactt aaatcaattt catgcttctt
     1981 ttcgtctttc atattgataa caattctacc tttatcagac gttcctttat acaatctata
     2041 tttttcgatt aagagttttc ttattttaaa gtccagttcc ttcaatgata cttcttcctt
     2101 ttgaataaaa aacaactcgt ttatcgaaaa gccattttta gcatcaactt ttttaacttg
     2161 tagacttgga ttacttacaa atccaaacac tgatctcaca tttttctttg ttataccgcc
     2221 aacgctatat atgccacctt ttatatctat taattcaggt actgcaaaga catcaaggcc
     2281 atgtgttttt tctttatatt tatttttatc gtcaccaagt aaaaatactt taaaagcttt
     2341 atttcgttgt ttaaagttta aaacgttaga gccatagtaa tcttcgcttt taacactaat
     2401 gtttgttggt tcaaaacttt cttcagaata gtattgatgt agcatattga tataatattt
     2461 gcgttgtgtc tcatcaagtt ctactttcgc gtgaccagtt tgactttctg ctgtaaacac
     2521 acctgtagtt aatattccta atgctaatgt tgctttagct atcgttgtta atttcatatt
     2581 tttttgctcc aatcttaatg tattggattg ttattattac gtaatttgaa ctatctgtct
     2641 ttattataag gtgcatcaaa acagactgac attaacgctc atttaaataa acttgaatca
     2701 ttattaatga aaatcaaacg tattttaaca ttcgtattaa ctgattttta atagcaacat
     2761 aaatattttt ttggtgaatc attgttcttt ccgattagtt ttaaatctta agctattggt
     2821 tataaagtgt acaaaaaagc agcttaataa cgtagtcaat gttcgatatg tttttattat
     2881 aaaaacacgt atcaaaatca ttaaacatgg gattattaaa ccgcttctta agctttcatt
     2941 ctatatatat cattgattat ttcaaattca cttcgatgtt tttaatttgt tcactattaa
     3001 tgacatctgc catacgctcg aaatctaatt tatcacttaa atcaatttca tacttatttt
     3061 catctttcat attaataaca attctacctt tatcagctga cccttcatac agtttgtatt
     3121 ttttaatcaa cagttttctt attttaaaat caagttcctt caatgacact tcttcctttt
     3181 gaataaagaa aaattcatca atagaaaaac cgtctttatc gtctattttt ttaactagta
     3241 agttcggcgt tcttagagac tcaaatattg attttacgtt tttctttgtt acaccactaa
     3301 cactaaatat tcttccatct aaatctacta attctggtac cgcaaagaca tctaaaccat
     3361 gtgttttttc tttgtattta ttttcatctt ttcccaataa aaacacttgg aacttttgat
     3421 tttgttggtt aaaaagcaaa acgttagagt cataataacc ttgactttgg ccactaacat
     3481 ttgttaactc ataactaggt cctgaatagt attgatgtaa cttgtttgta tcatataaac
     3541 ggttcatttt ttcatacttt ccttttgcat taaccgattg attttctgtt atcattacac
     3601 ttgttgttaa tattcctaat ataaatatcg ctttagctat cactgtaaat ttcatattca
     3661 tgtgctccaa tcttaatata ttggattgct tttattacgt aatttgaata atacaccttt
     3721 attataaagg gcattgagac agactgacat taacactcgt ttaattaaac ttaaatcatt
     3781 gttaatgaag agctaattaa tattcaatgg gcatcacttt caagagcaat gtaaattttt
     3841 agtatgctca ctcataacct gcgttataaa acgtaaaaaa aagcctaacg acaaggttgt
     3901 tttaaatacg ctccttaatg taaggtgtac gtatctattc aacatttcat cgttaagctg
     3961 cttcaaattt attactttag agtcattgat tacttacttt aaatttgttt caaagtcact
     4021 tcaatcttat taatatcctt actattcaac acatcaccca tgcgctcgaa ttgcaattta
     4081 tcacctaaat caatttcttg cttttctcca tctttcaaat tgatagtgat cttaccgtat
     4141 ttagtcgtac ctttatataa accataattt ttaactaaat gttgtctaat tttgaaatca
     4201 agttctttca gtgaaacttc ttctttatta attgaaaatg agtcaattga tgcatctaaa
     4261 tttccgccat acactttatt aacaaataaa tgtgtattag tttcagaaga tttgttattt
     4321 ttcttagtca caccaccaac agtagataat ctaccgtttg gatcaattaa ttcttttacc
     4381 acaaagacat ctttgccttc aatgccttct ttatatttct ctttatcttt acctaataag
     4441 aataattggt gattttgatt ttcttggtta aagcgtacaa cgttagaacc gttataattt
     4501 tcaaccttac cactaatatt actgaattca aaactttctg atgagtagta tcgatgtaag
     4561 tctttaatat catataaatg ttgtactctc tcttgctttt cttttgcttg aactgcttgg
     4621 ccttctgatg taatcacacc agtagttaat aagcctaatg ccaatgttgc tttagctaac
     4681 gtttttaatt tcacagtact attctcccaa tctatttata aatttgtctt aatatatttt
     4741 tatatgatta attcaattac gtaatacgaa caatctacta tcattatata agtgagatac
     4801 tgcgctatga attaacctct ttttaactat ttttgaacca acgttaagtt agttaatgaa
     4861 atcctaacag tattaattat tttttataac acaatactaa ttttttaact attcacactt
     4921 tccctcaatt taattatgtt ttccacattg tttcatgtca cgaaaaggac aacgcgcgac
     4981 tataagtatc aaccgttttt ccaaaaactt gatcataaac ccgctccttt tttcatcata
     5041 acaaaataaa aaagaagcta agcaatatgt tatcgcttaa cttccctaca attactagtc
     5101 tgcttgtcca aagattattt atattctagc tcaacattaa tttctttgat tttggtacca
     5161 tctatcgtgt cacccatgcg atgcggttgt agtttttttg taagttcgaa agtataaaac
     5221 ttatcatctt ccattttaac tacaatttta ccttttctat tattaacagc accatataat
     5281 ttttcttctt ccatcaattt ttttcttaat ttaaagtcca actcttttaa cgagatttct
     5341 tctttagtaa ttttaaattt actatcttta gacttttccg atgattcatc aatttttctt
     5401 gttactttaa catcgacttc tttactaaat tctttactat ttgtcttact taaaccacca
     5461 attgaatatt gtcttccatt aacagtttcc tcctcgatga ctaaaaatgc atcttgctta
     5521 tcatgctcac cttgagggta tttttcaata tctttaccaa gcaagccaac aaaataatct
     5581 tttcgatcct tccaaacata tactccttta tctgtcactt tctgtctata caaaccatca
     5641 agatttttta actctatact tggcttgcta tagtagtatt ttaacgattt ataattgtgt
     5701 tgaccttgag ttgattctgc cgcttttact gtttgacttt ctgttgttat tacaccagta
     5761 gctaacaatc ccaatactaa tgttgcttta gctaacgttt ttaatttcat agtactattc
     5821 tcccaatcta tttataaatt ttgtcttaat atatttttat atgattattt caattacgta
     5881 atacgaacaa tctactatca ttatataagc gtaatactaa gctatgaatt aacctctttt
     5941 taactatttt tgaaccaatg ttaagctaat taatggaatc ctaacagcgt taatctattt
     6001 tttaaactta acgcagcttt tttaactatt cacactttcc ctcaatttaa ctatgttttc
     6061 cacattgttt catgtcacga aaaggacaac gcgcgactat aagtatcaac tatttccaca
     6121 agttttattg gtgtttttat tattcatcga tacgcttcat tttcatctct ccaacacaaa
     6181 aaagaagcta agcaacttat gttgcctaac tcctctatac tatccatatt ttactattat
     6241 ccatatttca ttgaattatc taatgttggc ttctattttt tcaatatttc taccgtcaat
     6301 gacgtcactc atgcgatttg tttgtaattt tttattaagt tcaaacgtat aatagccgcc
     6361 atctttcatt atcactttta tcttactatc tttaggaaac tttttataca gatcaaaatt
     6421 ttgaattaaa tactgtctca atttaaagtc gagttcttta agtgaaatct cttctttata
     6481 aatgtagtgt actctaccgt acgtagcaat accgtcacct tcatctctct tgatttgaaa
     6541 tcttggtgcg tttatataat cataataagc gtcttgattt ttcttagtga caccaccata
     6601 tgaaaacact gtgccattac ggttttccgc ttctttaaca acaaatatgt ctaatcccgg
     6661 atttttacgt gctttaaatc tttcaatatc tttaccaaat atctgtactc ttgtgaattt
     6721 tctattttta tcaaagataa ggtaatgctt gccacctttg ctataacgat aaccagtaac
     6781 atttttaagt tccttacttg cgccactata gtaatctctt aagtcaaaga tatcttttgt
     6841 cacattttca tattttgctt tatgttcact cgcatttaca gtttgatgca atgacgttat
     6901 tgttcctgtt gctaaaatac ctaatgctaa acttgctttc gcaattgctg tcattttcat
     6961 agttgtatgc tccattcgta attattagat ttgttcgctt acgtctattg aatcatacag
     7021 ctttattata gttagcgtat ttgacctttc acattaaacc atgtttaata atcattgaat
     7081 cattattaag taaattaagg aatctataat gttcgttaaa taaaactgat cccgttgtgc
     7141 ttcacacccg atagataggg atttacagat aaattcaggt ctcttccacg tcatatttgg
     7201 acccatcgaa aattcgggtt ctcaaatcat cgaacataac aaaagaagct aagcaacatg
     7261 taggccgttg tcacttaact tcttgttttt ccgatgacag cttctattta gagaa
//
LOCUS       DAKTGS010000139         7181 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.139, whole genome shotgun sequence.
ACCESSION   DAKTGS010000139 DAKTGS010000000
VERSION     DAKTGS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.139"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..1152
                     /locus_tag="O0286_002391"
     CDS             <1..1152
                     /locus_tag="O0286_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCY0170736.1"
                     /translation="KVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFAY
                     HDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPIT
                     MTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDID
                     FKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQK
                     YSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDIY
                     NVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLT
                     NMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     regulatory      1371..1506
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1633..2676
                     /gene="ribD"
                     /locus_tag="O0286_002392"
     CDS             1633..2676
                     /gene="ribD"
                     /locus_tag="O0286_002392"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCY0170737.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            2683..3315
                     /locus_tag="O0286_002393"
     CDS             2683..3315
                     /locus_tag="O0286_002393"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCY0170738.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3326..4507
                     /gene="ribB"
                     /locus_tag="O0286_002394"
     CDS             3326..4507
                     /gene="ribB"
                     /locus_tag="O0286_002394"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCY0170739.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            4520..4984
                     /locus_tag="O0286_002395"
     CDS             4520..4984
                     /locus_tag="O0286_002395"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCY0170740.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5106..6107)
                     /locus_tag="O0286_002396"
     CDS             complement(5106..6107)
                     /locus_tag="O0286_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCY0170741.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            6219..6338
                     /locus_tag="O0286_002397"
     CDS             6219..6338
                     /locus_tag="O0286_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170742.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            6341..7168
                     /locus_tag="O0286_002398"
     CDS             6341..7168
                     /locus_tag="O0286_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0170743.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT         2568 a         1022 c         1336 g         2255 t
ORIGIN      
        1 aaagttcaag agatttatac aaattctaat atcgatgaaa cgaatttaac ttattacata
       61 tgtactacaa attcaggaca atcatggcaa gcgtacgatt atgtattttt aacatgtggt
      121 acatttgcct accatgatcc atataactta aagggtaaaa aaggatatat cgctacgcct
      181 tatccaacat ataatacgtt agacgaagta aatgaactgg acgatattgc gattattggt
      241 acaggccttg ccagtttaga tgttgtccgt tatgttgctg ctcatcatcc aaaattacca
      301 attactatga cgagtcgttc tgctcattta ccaagtgtaa gaggtacaat gatagacgtg
      361 acattcaagt acttaactaa agacaaatta aatgacatta aaaaacatca tttcggcaat
      421 gcaccgcttg atactttagt ttctttattt ttaaaagaat gtgctgatta tgacattgat
      481 tttaaaaaat tagtacatag acgtacagga aatcatattg cagatttgaa gtatgattta
      541 gcgcgtccaa cagaaatggg cattttccaa agtatgattg aacatttgaa agagaattta
      601 aattggatat ggaatagctt aagtattgaa gaccaacatc aatttaatca aaaatactca
      661 aagatgattc aattaaattc aaatccaatg cctccaagaa ctgctgaact aattattgaa
      721 ttaatagaaa ataagtcttt aattttgaaa aaagatttag aagatgtgaa acatgatggc
      781 aagttgtatt atttttcata caaaaaccaa gaatcagtag acatatataa tgttgtcatt
      841 aacgcgacag gtgcaaaaag tcatttaaat gaattggatc aagatgatca attaattaga
      901 aatcttgaaa atagacaaat tgtacaagca cacccaatgg gaggcataca aatcattccg
      961 gaaaccaatc aagttattag tccaaggttt ggaacattaa caaatatgat tgcgataggt
     1021 caaatgacaa acggtgttaa taaactacga aacggtgtga aaatgattgt agaacaagtt
     1081 gcgcatacag tatctcaatt atatgatgct ttagaatcga atgagcaaca acagcgcagt
     1141 gacaatcaat aatttgtaac tagaagataa taaagagaac gctctataga gacgaattga
     1201 aggtttgatt ttaatgtctg ttagtaagaa tcatatcaat gagatgccta tagtactcag
     1261 attatattaa attaaaaccg tcattaattg ttttttcaga aaacatatag tatcatttta
     1321 aatgtagttg acatactacg tactcaaata atctataaca atttcatata taattctttc
     1381 ggggcagggt gaaattccca accggcagta aataaagcct gcgacctgct aatatgtttc
     1441 atattagtgg ctgatctagt gagattctag agccgacagt taaagtctgg atgggagaaa
     1501 gaatgttaat tatcgacaaa gataatgtag cgtatttgta aaaatgtgta caaataggct
     1561 tatttaacga taaatttttc tccttgcatc ttaattcatg atgtgaggat ttttgtttat
     1621 agaggtgatc atttgagtca atttatggat tatgcgattc aacttgcaaa tatggtacaa
     1681 ggtcaaacag gtgttaatcc acccgttggc gctgttgtag ttaaagaagg taggattgtt
     1741 ggtattggtg cacacttgag aaaaggtgac aagcatgcgg aggttcaagc acttgatatg
     1801 gcacaacaaa atgctgaagg tgcgacgatt tatattacgt tagagccatg tagtcatttt
     1861 ggttcaacac caccctgtgt taacaaaata attgattgta agatagcaaa agtagtatac
     1921 gcaacaaaag acaattcgtt agacacacat ggtgatgaga cgttacgggc tcacggtatt
     1981 gaggttgaat gcgttgatga tgaacgggca tcacaattat accaagactt ttttaaagca
     2041 aaagcaaagc aactgccaca aattacagtg aaagtatctg caagtttaga tggtaaacaa
     2101 gcgaatgata atggacaaag tcaatggatt actaacaaag aggttaaaca agatgtctat
     2161 aagttaagac atcgacacga cgcagtgtta actggaagac gtacagttga attagatgat
     2221 ccacaatata ctacacgtat tcaagatgga aaaaacccta taaaagtaat attgtctaag
     2281 tctgggaata ttcattttaa tcagcaaatt tatcaagatg aatcaacacc aatttggata
     2341 tatactgaaa atccaaattt aacaaccaat caaacacata ttgaaattat ttacttgaag
     2401 tcttgcgatt taacaacaat tcttcacaat ttatataaaa gaggagttgg aactttgcta
     2461 gttgaggcag gtccaaccac tacttcagaa tttctccaat ctaattatat agatgaattt
     2521 attctctatt atgccccgaa attaattggc ggatctggaa attatcaatt ttatcaaaca
     2581 aatgatgtga ttgagatacc agatgcgaac caatttgaaa ttgttcattc cgagttatta
     2641 aatcaaaatg ttaaattaac tttacgaaag aagtgatgat gcatgtttac tggcatcgtt
     2701 gaagaaatag gtgtcgttaa aagtgttcaa attcgtcaat cagtcaggac gattgaaatt
     2761 gaagcacata agattacggc agatatgcat attggtgatt ccatcagtgt taatggtgca
     2821 tgtttaacag tgattgattt taatcagaca tcttttactg ttcaagtaat taaaggcact
     2881 gaaaataaaa cctatttagc agatgttaag cgacaatcag aagtaaattt agagcgtgcc
     2941 atgagtggta acggtaggtt tggtggacat tttgtcctcg gtcatgtaga tgaactagga
     3001 acagtttcaa aaataaatga aacagccaat gcaaaaatta ttacgattca atgtagccaa
     3061 catattaata atcagttagt taagcaaggg tctattactg tggatggtgt tagtctaacg
     3121 gtatttgata agcatgataa cagttttgac attcatctta ttccagaaac gaggcgttca
     3181 acgattttat catccaaaaa attaggagat aaagtacatt tagaaacaga cgttttgttt
     3241 aaatatgttg aaaatatttt aaataaagat aaagaccaat tatctgtaga taaattaaga
     3301 gcatttggtt tttaggaggg gtagcatgca attcgataat attgacagtg ctttaatggc
     3361 tttaaaaaat ggagaaccaa ttattgtagt agatgatgag aatcgtgaaa atgaaggtga
     3421 tttagtagcg gttactgaat ggatgaacga taataccatt aattttatgg cgaaagaagc
     3481 aaggggatta atatgcgcac cagtgtctaa agatattgca caacgtttgg atttggtaca
     3541 aatggttgat gataactccg acatctttgg tacgcaattt acagtgagta ttgatcatgt
     3601 agatacaaca acaggaatta gtgcttatga acgtacattg actgccaaaa agctcattga
     3661 tcctagtagt gaagctaaag attttaatcg tcctggtcat ttatttccat tagtagcaca
     3721 agataaaggc gtattagcta gaaatggaca cacagaagcg gctgttgatt tagctaaact
     3781 tactggtgcc aagcccgctg gtgtcatttg tgagattatg aatgatgacg gcacgatggc
     3841 gaaaggacaa gatttacaaa agtttaaaga aaaacatcaa ttaaagatga ttacgattga
     3901 tgatttaatt gaatatcgta aaaaattaga accagaaatt gaatttaagg caaaagtgaa
     3961 aatgcctaca gatttcggaa catttgatat gtatggtttt aaagcgacat acacagatga
     4021 agagatagtt gtactgacaa aaggtgcaat tcgacaacat gaaaatgtac gcttacattc
     4081 tgcgtgcctt acaggcgata ttttccatag tcaacgttgt gattgtggtg ctcaacttga
     4141 atcgtctatg aagtatatca atgaacatgg tggcatgatt atttatctac ctcaagaagg
     4201 tcgtggcata ggattgttaa acaaattacg cgcatatgaa ttaattgagc aaggatatga
     4261 tacagtaact gcaaatttag cattaggttt tgatgaagat ttgcgagatt atcatattgc
     4321 tgcacagatt ttaaaatatt ttaacatcga acatatcaat ttattaagta ataatccaag
     4381 taaatttgag ggattaaaac aatatggcat tgatattgca gaaagaattg aagttatcgt
     4441 accagaaacg gtacataatc atgattatat ggaaacgaaa aaaataaaaa tgggtcattt
     4501 aatataggag gattttaaca tgaattttga aggtaaatta attggaaaag atttgaaagt
     4561 tgcaatcgta gttagtcgat ttaatgattt tatcactgga agattacttg aaggtgcaaa
     4621 agatactttg attcgacatg atgttaatga agacaatatt gatgtagcat ttgtgccagg
     4681 tgcgtttgaa attcctttag tagctaaaaa attagcctca tcaggaaatt atgatgcagt
     4741 aattacatta ggatgcgtaa ttcgcggtgc tacgtctcat tatgattatg tttgtaatga
     4801 agtcgcgaaa ggtgtttcta aagtgaatga tcaaactaat gtaccagtca tatttggtat
     4861 tttaacgact gaaagtattg aacaagctgt ggaaagagca ggtacgaaag ctggcaataa
     4921 aggtgctgaa gcagcagtaa gtgcaattga aatggctaat ttattaaaat ctataaaagc
     4981 atagagcact gttgtgagtt cataatcaaa gatttataaa tgtttcagaa ctgccaatat
     5041 aacatttagg agcctagaac attgattatt gttccagact cctaaattat tgtttatgtc
     5101 ttgctctatt tacggcataa ttttttaatt gtacttaaac ctaacataac tgtagctcct
     5161 aaagctgcaa ttatgccaac acgttttaag ccagcaggtt tcacaaattc ttttacagca
     5221 agagataggt tttgtgggcg ttctgctaat cttctcataa aatacgcaaa ccaatcatcg
     5281 ccataaggta cataaatagt gaaattatag ccttcatttg cgatttcttc tgctaactct
     5341 gatctaaaac catagagcat ttggaattcc atacgatctt tttcaatgtg attttctttc
     5401 ataaattgtt ttacatgatt aatgatgcga tggtcatgtg ttgcaattga agtgaaattg
     5461 cgtgcgttta acaaacgttg ttcaattatt ttgatgtaat ttgcatctac gtcttcctta
     5521 gattgaaatg caattgattc gttttcttta tatgcacctt taaccaaacg taatcgtaaa
     5581 tcttggtact tatcaactaa ttcgtggcta tcgtataaat atgcttgaat tacagtacca
     5641 acatttctaa attcgccttt taagcgatct aaaacttgaa caatttgttg caggctagca
     5701 tatttttcag tatcaatatt tatatgcata ttgttgtaag tatttgcttt aagtaaaatc
     5761 tctcttaaat tttggtaagc taattctaag tcgaattctg cacctaactg actcaattta
     5821 acagacatat gtgcctttac gccatgttga tgaagcgcgt ccataattgt taaaatttgt
     5881 tctttagcat gattactttc ttcaactgta ccgacaaatt cccctaaatt gtctaccgta
     5941 acagcgatat tcttgtcatt taagtattcg attgtattaa ttaactctgg aattgtattt
     6001 ccggcaacga ctttattggc gcccaaacgt gggcccactt tttttgctgc gttgtttaaa
     6061 aaactattat tagataatcc gataaaaaaa ttctttaata gtgccattgc tactcctcca
     6121 aacccttgtg tttacaataa gtttagcatg aaaaaaatat tcgagaaagc gttttcatca
     6181 aaaacaatca aaaataatta tgtataatta cactattatt gatgttgatt tcatttatat
     6241 ataataatat ggacaacaat aatactataa atagaaatct acatagtgaa tttgctataa
     6301 ttatgttatt gaagattaat gaaaaagggg cagtataata atgtggaagt gggaagctga
     6361 aaatgatgcg aaaggcgtcg ttgttattgc tcataatata ttagaacata cgggaagata
     6421 tgcatatgtt attacgatgt taagaagaaa tggctatcat gtgattatgg gagatttgcc
     6481 tggtcaaggt caaacttcaa gagctaataa aggacaaatt gaaaactttc aaacgtatca
     6541 cgaaagtttg ctagactggt taaagatagc taatgaatat aaaattccta cttatgtatt
     6601 aggggtagga cttggcggtt tgattttatt aaatttactt gaaaaagtag aattgccaat
     6661 cgaggggatg atgttaattt ctcctatgct agaacttcaa aagaatggaa aaaatcgtaa
     6721 agataaactt gtttcaaata taggtaaaat ttcaaaagac acacgtttta atgttggtgt
     6781 agaaccaaaa gatttaacac gtaatttaga aattgttgaa gaaacagtta acgatggttt
     6841 aatgttaaaa aaagcaacgt atcattggta taatacaatt aatgagacga tgaaagatac
     6901 gatggctcat attcatgaca ttcaaccaat gccaacgcta ttaatgtatg gaacgaagga
     6961 tttaattgtt gatactaggg caattgatga gtttaaagaa aaatatcaaa cacctgaatt
     7021 atattttaaa gcttggcaag gtttttacca cgaggttcac aatgagccag aacgtgatga
     7081 agtaatgcgt tatattctga ctttcttaaa taacagcgtc aatacaatgg gctttattgt
     7141 tgaagatgac gaaattgtag aaatttaata ttctaaaaag g
//
LOCUS       DAKTGS010000140         7088 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.140, whole genome shotgun sequence.
ACCESSION   DAKTGS010000140 DAKTGS010000000
VERSION     DAKTGS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.140"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            103..451
                     /locus_tag="O0286_002399"
                     /pseudo
     CDS             103..451
                     /locus_tag="O0286_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            462..656
                     /locus_tag="O0286_002400"
     CDS             462..656
                     /locus_tag="O0286_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170744.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            697..1047
                     /locus_tag="O0286_002401"
     CDS             697..1047
                     /locus_tag="O0286_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170745.1"
                     /translation="MLEMISNGKMTMKLNNVKQKRHILCTNEYNNKKNNSSLLPSYTI
                     IDSNESEKMTKKEFIDIPVLFDDEGNFRIKQVIDYKKIIGKSYVNGKYIETKLGKVHY
                     SKTGFHVVPYIKKE"
     gene            1054..1245
                     /locus_tag="O0286_002402"
     CDS             1054..1245
                     /locus_tag="O0286_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCY0170746.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQ"
     gene            complement(1794..2819)
                     /locus_tag="O0286_002403"
     CDS             complement(1794..2819)
                     /locus_tag="O0286_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCY0170747.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            3089..3286
                     /locus_tag="O0286_002404"
     CDS             3089..3286
                     /locus_tag="O0286_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170748.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEATNLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            3344..4825
                     /gene="cls"
                     /locus_tag="O0286_002405"
     CDS             3344..4825
                     /gene="cls"
                     /locus_tag="O0286_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0170749.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            5000..5908
                     /locus_tag="O0286_002406"
     CDS             5000..5908
                     /locus_tag="O0286_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170750.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
     gene            5877..6608
                     /locus_tag="O0286_002407"
     CDS             5877..6608
                     /locus_tag="O0286_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170751.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDV
                     KPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVK
                     TMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLIS
                     FAIILLYALGSIIAVYCISHFKRAE"
     gene            6612..>7088
                     /locus_tag="O0286_002408"
     CDS             6612..>7088
                     /locus_tag="O0286_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0170752.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQK"
BASE COUNT         2640 a          922 c         1071 g         2455 t
ORIGIN      
        1 ggcaattggc gggacaagtt ctttaaagag cgtgaaggta aatatcaagt agaagtaaaa
       61 gaagcataat tacaggaaaa agctaaaaac cagatgaaag aaatgattaa aaatggtaaa
      121 ataaaaatag aaataaattg cggaaaaata aaatagatat atgttaggtc atcacctata
      181 taatgaaaat aaaaaaagag ccattttaaa taataagaaa ttgcctagct atacaatact
      241 ttctatagat ttattgaatg aattgttaag agaaaaaatg tcaacaggca atctaatatt
      301 aagtgatgag cgatttgatt tgaaagagat tattaatttt aatcaaatta ttggaaaaat
      361 acatatcgaa aatgtgtata ttgaaaccag aaagggaaaa gtgcattatt cgaagacagg
      421 tgctcatata gtaccttata ttgataagta ggtgaaaagt attgagaatt gaagatgcat
      481 atcgtaaaga tgttattatc acgcttttga ataatgaaga atacgaaggg tttgtaactg
      541 actatgaaaa tgaattcgag agcgaaacag gaaatcttgt tgtagatata cagactgatt
      601 ttgctgttta ttcgtttgat gaaactgaga taaaaagtat tagattatta aaataattac
      661 aaaatagcat cctttctaca aaaagctaaa aaagaaatgc ttgaaatgat aagtaatggt
      721 aaaatgacga tgaaattgaa taatgttaag caaaagcgtc atattttatg cacaaatgaa
      781 tataataata agaaaaataa ttcatcttta ttaccaagtt atactataat agattctaat
      841 gaaagtgaaa agatgactaa aaaagaattt attgatattc cagttttgtt tgatgatgaa
      901 gggaatttta gaataaaaca agttatagat tataaaaaga taataggtaa atcttatgtg
      961 aatggtaaat atattgaaac gaaattaggt aaggttcatt attctaaaac tggttttcat
     1021 gtagttccgt atattaaaaa ggagtgatta aatatgaatt tacagtcata cataggcaaa
     1081 ttagtcaaat taacgcttac taataataaa atattaattg gaaaagtgat agactttgat
     1141 gataaagttg ataattttga tggttataat tcgatagaaa ttgatacagg tagaattaca
     1201 tatgatatat cagaaaataa aattaaaact attgttttac aataatagca tcctttctac
     1261 acagataaag agaaagcggt gctatttttt gcgcttttat aacaactaag gatacaagac
     1321 acataacttc aacaatatcc tcatctaaac ctatcgattt cctaagctca attttaaaca
     1381 caaagtttat ttgaaatgat gcacacctat aaaaatctta atcatcgaaa agtcacaaac
     1441 atctccatat taaaaactta aatagtgcat tccatttaaa ctaaacatcg atattcctac
     1501 caatatagac ttagtaattc gattgttaat acattacttt cataaaataa ctacaaccat
     1561 cataatactt ctcaaatgtc gtcaaattaa agtaaataca gcacaaatta acatttttaa
     1621 atatataaaa taaaacaaga tactcaataa tctgtagtac cttgttttgg aataatgtaa
     1681 ttttatatca tcaacggcta caaatgagca gtttcatata taaataaaca catctactca
     1741 actgatctta attaattaat aaatgattta ccagatgaca tataacagca ttattagatt
     1801 aattctaata gtttatttaa attttcttcg gttgtcgccc aactggttgc gaatctaaca
     1861 acacgatgtt gatcatcgta tttttcccaa acagcaaatt taactttttg ttctaactct
     1921 gctattttct cgttacttaa aataaaaaat tgttgattgg ttggagaatc aaagtaaaga
     1981 cgatagcctt tatttttaaa cccgtctttc atcttatttg ccatttcgat agcatgtctg
     2041 cttatattaa aatataaatt gtccgtaaat aattctaaaa attgtatgcc tgttaaccgt
     2101 ccttttgcta aaagggcacc gtgatgcttg attcgagtgg taaattgttt cggttcatta
     2161 tttttcgtaa aaacaatggc ttccccgcat aatgcaccta tcttcgtacc acctatataa
     2221 aatacatcac aatatttagc gatgtcttta atagtcatat ctgattggtc actcatcaat
     2281 ccatacccta atcgtgcacc atccataaat aatggaagct gatattgctt acatactttg
     2341 cataactctt ccaattctga tttagagtat aatgtgccat attctgtagg atgagaaata
     2401 tataccattc ctgggaatac catatggtcc tttttaaaat cacttttaaa tgtctccatg
     2461 taagtttcaa catctgaagc actaactttt ccttccttag agggtatagt aattacttta
     2521 tgtccactat attcaattgc accgccctca tgcacagcaa catgaccagt gtctgctgaa
     2581 atgacccctt cgtaactttc taacattgaa ttaataacaa cctgattggt ttgtgttcca
     2641 cctactaaaa aacgaattgt agcatttgga cagtcaattg tatctttaat cttttcaatt
     2701 gcctgagctg tgaattgatc aaagccatat cccgaagctt gtacaagatt tgtatctact
     2761 aatcgtttta atactttttc atgagcacct tctaaataat cattttcgaa tgaaatcact
     2821 acatttcccc ctaaaactaa tatcaacatt ttaataagat aaaccaattt caaaactagt
     2881 tcgatattta aaatgtatta tgaatggtta aagtttgtat cgcattatcg cgaagttgaa
     2941 taaatatatt atacatcaaa caatacatta tgaaaattaa ttaatttttt atcacagcga
     3001 cttttcatgc tggaacacat gtattaatga ttgaatttta ctgttaaaag ttctatgatt
     3061 taggaaatag agaaaagagg ggatttaaat ggaacaaatt aaacttaaaa cttttacagc
     3121 tgagacttta gagttattag aaaaaaacat taatgctttt ttaagttctg aagaagctac
     3181 aaatttaaaa ttagtaaata ttactattaa agaaatagaa gaaagaacat tcccaaataa
     3241 tgaagaagaa ttcaatgcaa ttttaacttt atctgtgaat aaataatatg acgtgaaagg
     3301 tgcaaaatgt ttttttgtgc ctttctttat aggagaaaag actatgcgat ttacattttc
     3361 aaacgattta ggaacgttat ttactattat tttagccatt ggattcatca ttaatttagt
     3421 attggctttt attattatct ttttagaaag aaataggcgt acagcgagtt caacttgggc
     3481 atggctattt gtactttttg tcttaccatt gattggtttt attctttact tgttttttgg
     3541 tagaaccgtt tcggcacgca aattgaataa aaacaatggt aacgtgttaa cggatttcga
     3601 tggactttta aaacaacaaa tagaaagctt tgataaaggt aattatggta ctgataacaa
     3661 acaagttcaa aaacatcatg atttagtacg tatgcttttg atggatcaag atggtttttt
     3721 aactgaaaat aataaagttg atcatttcat tgatggaaat gatttatatg atcaagtttt
     3781 aaaagatatt aaaaatgcaa aagaatatat ccatttagag tactatactt tcgctttaga
     3841 tggtttaggt aaaagaattt tacatgcttt agaagaaaaa ttgaaacaag gtctagaagt
     3901 aaaaatatta tatgatgatg ttggatctaa aaatgttaag atggcaaatt ttgatcattt
     3961 taaatcgtta ggtggagaag ttgaagcatt ttttgcttca aaattaccgt tattgaattt
     4021 cagaatgaat aatagaaatc atagaaaaat catcgtaatc gatggtcaac taggttatgt
     4081 cggaggattt aacattggtg atgaatatct aggattagga aaattaggat attggagaga
     4141 tacgcattta cgtatacaag gggatgcggt tgatgcactg cagttgcgat ttattttaga
     4201 ctggaattcg caagcgcacc gtccacaatt tgaatatgat gttaagtatt tccctaaaaa
     4261 gaacggacca ttgggcaatt caccaattca aatagctgca agtggcccgg ctagtgactg
     4321 gcatcaaatt gaatacggtt atacaaaaat gattatgagt gcaaagaaat ctgtatattt
     4381 acaatcacca tatttcattc cggataattc atatataaat gccattaaaa ttgctgctaa
     4441 atcaggtgta gatgtacatt taatgattcc atgtaagcca gatcatccat tagtatattg
     4501 ggcgacattt tcaaatgcct ctgacttatt atcaagtggt gttaaaattt atacgtatga
     4561 aaatggattt atacattcta aaatgtgctt aattgatgat gaaatcgtat cagtgggcac
     4621 agcaaatatg gactttagaa gttttgaatt aaattttgaa gtaaatgcct ttgtatatga
     4681 tgaaaatctt gctaaagatt taagggtggc ttatgaacat gatattacaa aatcaaaaca
     4741 actaaccaaa gaatcatatg ccaatagacc gctgtctgtt aaattcaaag aatcgttagc
     4801 aaaattagtt tcgccaattt tataatttat ttgtaaggag tctcgattat agaggctctt
     4861 tttattttgc ccaaaattaa aataacgata aatatattat gtaaatctaa taatgacatt
     4921 tgtcatttca aatctatgac aaagtgttct attttaaatc taataaattt actaatatta
     4981 aattaagaaa aaaatagagg tgattaaatt gattcaaata tctaatatca acaagtcatt
     5041 taataaaaga tgtgttctaa aaaatatttc gttcgatatt gaacaaggta aatgtatcgc
     5101 tttaattgga aaaaatggtg ctggaaagtc aacgttaatt gatatattaa ttggtaatgt
     5161 taatgctaat tctggtgaga tatttgataa agacaagtta ttacaaagtg aaaatcgcag
     5221 tataatgttc caaaaaacga tgtttccaga tcaattaaaa gttattgaga ttatcaactt
     5281 atatcaatca ttttacgaaa atccattacc attggaagaa ataatagaac tgacgaaatt
     5341 tgattctagt caactgaacc aatttgtaaa taaactttct ggtggtcaac aacgattact
     5401 cgattttgta ttatctttaa tcggacaacc acaattgatc ttattagatg aaccaacatc
     5461 gactatggat atagaaatta gagaatattt ttggtcaatt attgaaaatt taaaagaaga
     5521 taatcgaacg atactctata catcgcacta tattgaagaa gtcgaacgta tgtcagacaa
     5581 aattattctc attgaaaatg gagaaataat acttaatgat tcaacgtcac atattagaac
     5641 caatcagcaa tctcagatta cgttatccga tgaatatata agaaagttaa aactagataa
     5701 agatgattta gttattcaaa aaaatcataa tggcactatc aaaattatta cttcaaatgt
     5761 aaatgatacg attttatatc ttcaacaact tcatattaat ttggatgata ttgaaataca
     5821 aaaagtctca attgttgatt catacttcaa caataaaaag caaaggggat ctaattatga
     5881 tactaagtta cttgaaaatc gaatttaaag ttataatgcg taaaaaaaca acattaatat
     5941 tatctatttt atttcctgtt atattctata tattatttac ttcgatattg gaattgccgg
     6001 aagatgttaa acctaaattt tataaagagt atatgtatag tatgacggtt tatagtttgt
     6061 taagttttag tttactaact tttccattag atattattaa tgaaaaacaa aatgaatggc
     6121 gccaaagatt aatggtaaca ccatttactt ttactagtta ttatatttca aaagtagtga
     6181 aaactatgct gcaatttgca atagcgatat tagttatttt tatggttgga catttttata
     6241 aaggtgttgc aatgagtgca gttcaatggt tagagtcagg aatattttta tggttaggtg
     6301 cgtctctatt aataactttt ggcatattat tttctttgtt aaatgatatt caaaaaacaa
     6361 gtgctttagc taatatcgta acaattggtt tagcagtatt aggtggattg tggtttccga
     6421 taaacacatt tccaaattgg cttcaacatg ttgctcatgt tttaccgagc tatcatttgc
     6481 gtaaactagg tgtagatatt gcttcaaatc atcatatcaa tttaatatca tttgctataa
     6541 tactcttgta tgctttaggg agtataatag cagtatattg tattagtcat tttaaaaggg
     6601 cggaataaaa tatgaaattt ttaaaagata cttcaattgc tgaaatatcg tctatagttt
     6661 atctgatttt tcctattgcc ggtatatttt ttaatgaagt atatggtccc aaatggttgt
     6721 atattatatc agtcattgtc ttttcgttgt cgtatcttat attagttata gtaaataata
     6781 gacttaatac attaatgttt tacattttgt tgattattca ttattttatt atttgttatt
     6841 ttgttttcag tgtacatcca atgctaagtt tgtttttctt ttatagtgct tttgccgttc
     6901 catttacttt taaaaataat gttaaaaaaa cggcaactaa tcttttcata ctaacaatga
     6961 ttatatgtac aataataacg tacttattgt ataacaacta ttttgttgca atgatggttt
     7021 attatgtcgt tatatcgtta ataatgctag ataattttaa aaaaatgaaa aaccgtgaat
     7081 atcaaaaa
//
LOCUS       DAKTGS010000141         7069 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.141, whole genome shotgun sequence.
ACCESSION   DAKTGS010000141 DAKTGS010000000
VERSION     DAKTGS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.141"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            70..288
                     /locus_tag="O0286_002409"
     CDS             70..288
                     /locus_tag="O0286_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCY0170753.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(369..1355)
                     /locus_tag="O0286_002410"
     CDS             complement(369..1355)
                     /locus_tag="O0286_002410"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0170754.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            1554..1730
                     /locus_tag="O0286_002411"
     CDS             1554..1730
                     /locus_tag="O0286_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCY0170755.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            1745..2347
                     /locus_tag="O0286_002412"
     CDS             1745..2347
                     /locus_tag="O0286_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0170756.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            2580..2725
                     /locus_tag="O0286_002413"
                     /pseudo
     CDS             2580..2725
                     /locus_tag="O0286_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            3264..3365
                     /locus_tag="O0286_002414"
     CDS             3264..3365
                     /locus_tag="O0286_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170757.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            3375..3647
                     /locus_tag="O0286_002415"
     CDS             3375..3647
                     /locus_tag="O0286_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCY0170758.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            3691..5655
                     /locus_tag="O0286_002416"
     CDS             3691..5655
                     /locus_tag="O0286_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCY0170759.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKEN
                     KLSIQFINKNDCRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIIL
                     FDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVIFIGILTNVILHSHYIMML
                     FATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            5658..5978
                     /locus_tag="O0286_002417"
     CDS             5658..5978
                     /locus_tag="O0286_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0170760.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            5975..6616
                     /locus_tag="O0286_002418"
     CDS             5975..6616
                     /locus_tag="O0286_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170761.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(6704..6994)
                     /locus_tag="O0286_002419"
     CDS             complement(6704..6994)
                     /locus_tag="O0286_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170762.1"
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT         2591 a          934 c         1076 g         2468 t
ORIGIN      
        1 gaatattcat tgatttcaaa attagaaaaa tgtatactta ttataaatta aagaaaggta
       61 gtggtttcta tgaaatacaa tactaatgtt aaacatacaa ctttagaagc gtttgtcaca
      121 actgtcaatg atttgggtat tgaattaatt atcaatgaag cacttcgaga ggtaagaaaa
      181 cgacagctca tagaacttat agatgacgca ctcgtcaata aagatgaagc agcatttaat
      241 caatatacgg cagaatacaa aaatttggag gcatttctcg gtgaataaca ttgattcgaa
      301 ttccataacg caatcataaa aggactattc ttaacatctc atacatgtaa gaatagtcct
      361 ttaatcgttt atgacattaa tctaattaat tcatgtctat ctatgtcacc gaaataatga
      421 tataaatcat attctgatag tgcttcttct atgtgctcaa agtcgtgtag acagccaact
      481 aatgcatttt caagatcagt gacatctccg acaccaaaga aatcaccgaa tatttttgca
      541 tgttcgattt tacctcgttt aacatcaaac ttaatttgta caaatccttt ttcaaatttt
      601 tcctcgcgtt caaagttata tttagggttt ctaccataat tccattccca agttctatat
      661 ttgtcgttac ttaacttttc aatattttcc caatcttcat ccgttaattt atattcttct
      721 acttcagttt ctccaaagat agttttcaag atgatttttt taaattcttc aatttctaaa
      781 gggtcattta aaaattcttg aatgtttgct acccgtttac gaacagattt aatacctttt
      841 gatttaatct tagctggatt aacttttagt gcattctgaa cttcgtctaa atcactattt
      901 aacatcaacg taccatgact aaacattcta tttttaactt taaccatagc attccctgag
      961 attttcgctt gtccaacttg aatatcgtta cgaccagtta attcagcatt tacgcctaaa
     1021 gattgtaatg cttgaacaat tggttcagtg aatttttgga aattgtggaa actgttacca
     1081 tcatcatctg ttataaaact aaagtttaaa ttgccagtat catgataaac agcgccacca
     1141 ccagaaattc ttcttactac atcgatgttg tgagcatcga tatatgtctg atttacttcc
     1201 tctatcgtat tttgattctt tccaacaatg atagatggtc tatttatgta aaataaaaag
     1261 taactttctt ctgctggtaa attttttaaa acatattctt ccattgctaa gtttaaagtt
     1321 ggatctgtaa tattattatt actaatgaat ttcattacaa tctctccctt atatctatat
     1381 atattctcta cttatttatg ccttaacttt gctcaaaatg ccaattatat actcacaaat
     1441 cattctaaat atcggcttaa agttacattt tattattttt gattgttaat agtttatttg
     1501 ttaagtgaat tttagtacaa tataatttag ttatgattta aggaggactt aaaatgactg
     1561 ttgcagaagt gggtaacatt gttgagttta tggatggatt aagaggtcgt gttgaaaaaa
     1621 tcaacgataa ctctgttatt gttgacttaa caattatgga aaattttaat gaccttgatt
     1681 taccggaaaa aactgttatc aatcataaac gatataagat tgttgaataa gaaggtaagt
     1741 tataatgaat aaaatctcga aggctttaac ttggtttatt ataagtttca ttatatttca
     1801 tctcatatta tttattatgt ggggcgaaca ccaagaatac tggtatttat atacaggtat
     1861 aatgctaatt gctggtatca gttatgtatt ttatcaaaga gatattgaat ctaagcggtt
     1921 gcttacatca attggtgttg gtattattac ggcaattatt ttaattatgc ttcaactttt
     1981 attctcactt ataacttcta atttaagtta tagttcatta attaaagaat tagcaagaac
     2041 aggtgtcaat tggaagtggc aaatgttagt aactttactt tttgtcattc catgtcacga
     2101 gttatatatg agaactgttt tacaaaaaga attaacacat ttttcaatac cgaaatgggt
     2161 agctatatta ataactgcaa tttgttctag ttcattattt atttatttag ataattggtg
     2221 gatagtaaca tttatatttg ttgcacaagt gatattatca ttaagttatg agtatactcg
     2281 acgtatagca acaacatccg ttgcacaaat tgtagctatt atattgttat taatttttaa
     2341 tgcttaaata agcttttctt agacgtatgt gttctaagaa aagctttttt gcttaagaat
     2401 agcaatacga agattcgagg catgcttcat tatagtggta atatttttta acagcaagcg
     2461 gttattattg agattaggtt ttagtcagtt tttattacta aatattttat aaaaatggta
     2521 taatattttt gagcaagttg aattgatggt ggctatctga gtaaaggggg gtggtgccta
     2581 tggcattact taacactttt gaaaggaaaa gcctattgtg atatctattg caaacgcatt
     2641 acatttaatg ttaagtttcg gtatgtttat cgtcactttc attggtatag tagtagcaat
     2701 aataaattta agcaataaaa aataaccatc atttcaaaac tttgacatag aatgatggtt
     2761 aaagaaaatc tcatttagtc accgtctttt taacgggctc attaggcgac atgttcctgc
     2821 atgtcgtctt tttacatttt tatagtaaca tatcttttat ttaattgtct atttttatta
     2881 gatgtgtaac aaaattttga agtattttcc gtatattgcc tgctaaacat gtcagatttg
     2941 aaaggaaaat catactgtgg tagaaatcaa ttatgattat atggtagtac ttataaataa
     3001 gtgatttcta ttgaatatgt aatgaagttc atatattaat ataactttca ttgatataga
     3061 agtcgcaatg attaatttaa acaataaaaa acaaccatca tttccaaact ttgacaacga
     3121 atgatggtta aagaaaatct catttagtca ccgtcttttt aacgggctca ataggcgaca
     3181 tgttcctgca tatcgtctat ttatacactg cacaatttaa taataataca attatacttt
     3241 gatgtccatt taacataaag tggttgaaat ttgcagcaaa taaattaacc ttgactattt
     3301 tcagaaaatt ctgtataaat aacgaaaaag agatatggat taaatatgaa gaggtgtttt
     3361 tatagaagaa taaaattttt ggcactatat tatatttaac tttagcactt ggattatcaa
     3421 cagcagctta tgcatctaca gaatacgcag aaggaggcac ttggagtcat ggtgtcggca
     3481 gtaagtatgt ttggtcgtat tattatcatg gtcataaagg acatggtgca acagctattg
     3541 gaaaatatag atcatttagt ggttatacaa gagctggtgt aaaagcaaaa gcatcagcta
     3601 ctaaacataa ttggtgggtc aatagagcgt attataacat ttattaaata ctaaaaaatc
     3661 atagaaaata aacaacgcaa gaggtacaac atgaaatggt tcaaattaat acttgatgtt
     3721 acaatcttca ttctgattgc tatattgtta tttgtttata catataaaga aaacgaagaa
     3781 atattgcctg atactaaata ccctatagcg gtaactgact ggaataaaaa atatagtaaa
     3841 aatgagattt ataaacgtat agatcaattc gctaaaaatg agaacgtagc aatctataaa
     3901 tcaacttcaa attatacaaa caaaaacgta gataaagata tatatgtatt taataaatca
     3961 aaagcagcaa ctatcactcc ttttaatgct aaatataaca ttcattattt aagtgacgac
     4021 gaagtattaa aaaaagatat caaagggagt tattttgtaa aagacaaaaa ttttgacgtg
     4081 tctaaattca taaatttttt aaaagaatat ggtgttactg ctgaatcata caaaatagat
     4141 catatgatga ttgccgttgg tgtcattaaa caaatgaata tagtagttcc attatcctca
     4201 cttttaatcg tttattttat ttattatatt ttcgaaaaga acattaattt caaagcgtat
     4261 gcgattaagt atttaaatgg ttttacatta agaaaaataa ttttcgaaaa tttttcaaag
     4321 aaatgcacgt attgggtaac gttaatcata actcaaattc ttttaactac aagtgtactt
     4381 tggatattaa attacacggg taatttagat ttattcatat taagaatagt tctgctttca
     4441 tgtcttttca ttttaacgat tagtgttatt aatttatgga ctttcttaat gttactaaat
     4501 ttaaatattg caaatatgat taaaggtaag cagcacttta aaacaattcg ttttattaat
     4561 acagtttgta aaagtattct cttagtacta atagctaatg taatgataga aaatactagt
     4621 gttattaaag atttgaataa aataaaagaa actgagaaat attggaatgt attagatgat
     4681 tattacacga ttgaatttgc accttatcac gaaacaaaac aaagtttgat tgataatatg
     4741 gtgcgatcag aacaattagt aaaggctagt gaagcagaaa ataatgcgat tttattcaaa
     4801 ccaaagggtg actccgttga caatgacaac ttttcgcctg atgaggggaa tgtaatatta
     4861 gtaaataatc agttctggtc gatttattac aagcagtttc aacctgatat tccgataaaa
     4921 aatcaaaaaa ataatgtcga agtaattatt ccacaaaagt ttcatgcaat gcgtaatgaa
     4981 atcaatcaag catatcattc atggtttgaa tttgtacaaa ataaaaataa taaagagaat
     5041 aagttatcta tacagtttat caacaaaaat gattgtcgaa ttttttcatt tgatgcacga
     5101 gatagtcgcc atttgtcatt tatagaggcg ccaatcattg tgaatgttca ggcatcagat
     5161 ttatcgaatg atttttatta tgccatgatc agtcaaggcg ggtatttatt caaaaattat
     5221 gacgcgctag taaaaaatat tgaaaagtat catcttgatg gggaaatcag tggaataacc
     5281 aattataaag atagcgtgat ggaaatgtat catgaaaaca atttgaaatt aacagtactc
     5341 aacttttcac aaatcattat cgcaatcatt ttaataatta ttattttatt tgatgtgaaa
     5401 tattattttg aacagcatcg aaaattactc gtaatcaaaa agctatatgg ttattcaaca
     5461 ttaagagcca attaccaata cttattaata aataatatag ttgttatttt tattggaata
     5521 ttgacgaatg taattttaca ttctcactat ataatgatgt tatttgcaac gattcttgtc
     5581 gttcaaatat tattgcagat ttgcagccta tactatcatg gccgacgttt taatgaagtt
     5641 atcaaggagt tttaactatg aaatttatca ttgcaatatt attaggatta atattatcca
     5701 ttactattgc ttttacaatc atacatcatc ctatacttga tcgttttaat cctttcttaa
     5761 aaacggagta tagttatgcc aaagtgccaa aaggtacgca acaatatgtt aatattacgg
     5821 cttatagtga aagaggggaa aagcttgatt ataaattaac atttaatgga ttttcaccta
     5881 gtagaacgta tgtcgaaata aagcataaag ggcaatatgt catatcgatc acatatgttg
     5941 aaaaagagga tacaccaaaa gaggtaagac aagaatgata gaattaaaag atttaaccat
     6001 acaaaaaggt aatacacata ttttgaagaa acttaatttg aaatttcaat gtggaaaatc
     6061 atatgcactt attggtaaaa gtggatgcgg taaatctaca ttattaaata ctattgctgg
     6121 acttgaaaaa acaggaaaac aatatgtcta ttttaatggt caattagaac aatttaaatc
     6181 taatttttac agagataaat taggatattt atttcaaaat tatggattaa tcgataattt
     6241 gacagtaaat gaaaatttag atattggatt agcatataaa aaaataagta agaaagaaaa
     6301 agaacaaata aagatacgtt atatagaaca gtttggtctg tcaaacagtt taaaaagaaa
     6361 agttcatacg ctaagtggag gtgaacaaca acgtgtcgct ttaattagaa tgatgttaaa
     6421 agatccgatt gttatgttag ctgatgaacc aacgggtgcg ttagatccta aaacaggaca
     6481 gatgattatt caatcattat ttggtttggt cgatgaaaat aaagtgctga ttttagcaac
     6541 acatgatatg gctattgcaa atcaatgtga cgaaataata gatttagaac agtatagaaa
     6601 agtagcatct atgtgatatc caaaagataa aattaaaagc caatcaatgg gaaatgttga
     6661 cttcaacact ccgtttgatt ggcttttata ctgtgttact aagttatgaa agtaacaacg
     6721 gcaaactgaa aaaaagcaat ataataatat ttaatacgat tagtaataaa tgtaattgat
     6781 ttttcgtttt aatatagcaa aaaacaatga ctaaagataa caacaatatc atgattgcac
     6841 ctgggaagaa ccataatgct gaagctgtgt accaaatcat tggtaacgcc ataataataa
     6901 aagatataag acaagaaagg ataccaataa tattataagt tctagtcaaa attacacctc
     6961 caattttcac tttaaaatga catgcgtaaa acaatcattt taaatacatt atacaataaa
     7021 attaaattgt gcggtagaag aatttgaaat taaattgaat tgtttttgg
//
LOCUS       DAKTGS010000142         6834 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.143, whole genome shotgun sequence.
ACCESSION   DAKTGS010000142 DAKTGS010000000
VERSION     DAKTGS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.143"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            127..1110
                     /locus_tag="O0286_002420"
     CDS             127..1110
                     /locus_tag="O0286_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCY0170763.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1301..2227
                     /gene="pstC"
                     /locus_tag="O0286_002421"
     CDS             1301..2227
                     /gene="pstC"
                     /locus_tag="O0286_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCY0170764.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2229..3146
                     /gene="pstA"
                     /locus_tag="O0286_002422"
     CDS             2229..3146
                     /gene="pstA"
                     /locus_tag="O0286_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCY0170765.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3193..4044
                     /locus_tag="O0286_002423"
     CDS             3193..4044
                     /locus_tag="O0286_002423"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCY0170766.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4051..4692
                     /gene="phoU"
                     /locus_tag="O0286_002424"
     CDS             4051..4692
                     /gene="phoU"
                     /locus_tag="O0286_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCY0170767.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4832..6646)
                     /gene="pepF"
                     /locus_tag="O0286_002425"
     CDS             complement(4832..6646)
                     /gene="pepF"
                     /locus_tag="O0286_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0170768.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT         2386 a         1072 c         1164 g         2212 t
ORIGIN      
        1 acattttctt aacaattttt tatgtaacat ttacagtttc taaaaatgag gttaataatt
       61 caaggttaag ataaagatgt aatcaataca aatactattt gttgttcata cagggaggat
      121 atttcaatga aaaaatggca atttgttggt actacagctt taggtgcaac actattatta
      181 ggtgcttgtg gtggcggtaa tggtggcagt ggtaatagtg atttaaaagg ggaagctaaa
      241 ggtgatggct catcaacagt agcaccaatt gtggagaaat taaatgaaaa atgggctcaa
      301 gatcactcgg atgctaaaat ctcagcagga caagctggta caggtgctgg tttccaaaaa
      361 ttcattgcag gagatatcga cttcgctgat gcttctagac caattaaaga tgaagagaag
      421 caaaaattac aagataagaa tatcaaatac aaagaattca aaattgcgca agatggtgta
      481 acggttgctg taaataaaga aaatgatttt gtagatgaat tagacaaaca gcaattaaaa
      541 gcaatttatt ctggaaaagc taaaacatgg aaagatgtta atagtaaatg gccagataaa
      601 aaaataaatg ctgtatcacc aaactcaagt catggtactt atgacttctt tgaaaatgaa
      661 gtaatgaata aagaagatat taaagcagaa aaaaatgctg atacaaatgc tatcgtttct
      721 tctgtaacga aaaacaaaga gggaatcgga tactttggat ataacttcta cgtacaaaat
      781 aaagataaat taaaagaagt taaaatcaaa gatgaaaatg gtaaagcaac agagcctacg
      841 aaaaaaacaa ttcaagataa ctcttatgca ttaagtagac cattattcat ttatgtaaat
      901 gaaaaagcat tgaaagataa taaagtaatg tcagaattta tcaaattcgt cttagaagat
      961 aaaggtaaag cagctgaaga agctggatat gtagcagcac cagagaaaac atacaaatca
     1021 caattagatg atttaaaagc atttattgat aaaaatcaaa aatcagacga caagaaatct
     1081 gatgataaaa agtctgaaga caaaaaataa taagacgcaa tttcaaatgt gtcttgaaac
     1141 atgattttga tggtgaatca ttatttagag tacaaagctt gatttatcga gacgctgatt
     1201 ttgacattca gttagtctac aagcttatca acttaaaata gtggttcatc attattttac
     1261 aaatctaatt attttgggag taatagaaag aggtttgatt atgacttcat ctactaatgt
     1321 taaagcttta atcgaaaaaa ataataataa aaaaggaaag cataatgaca aaattatacc
     1381 agttatttta gccgcaattt cagcgatttc cattttaaca acactaggta tattaatcac
     1441 attgctttta gaaaccatca cttttttcac cagaattcca ataactgaat ttctattttc
     1501 tactacttgg aatcctaccg gttcagaccc taagtttggt atctgggcat tgataatagg
     1561 gactttaaaa atcacagtta ttgcgactat atttgcagtt ccagtcgggc ttggtgcagc
     1621 aatttattta agtgaatatg caagtgaccg tgcacgacgc ataattaaac cgatattaga
     1681 aattttagca ggaataccaa caattgtgtt tggtttcttt gcattaacct ttgttacacc
     1741 agtattaaga tctttcatac caggtcttgg agagtttaat gctataagtc ccggcttagt
     1801 tgtcggtatt atgattgtcc ctctcatcac aagtttgagt gaggatgcaa tggcatctgt
     1861 accaaataaa attcgagaag gtgcctatgg acttggagca actaaattag aagtagcaac
     1921 taaagtcgta cttcccgcag caacatcagg tattgtagct tcaatcgttc tcgcgatttc
     1981 aagagcaatt ggagaaacga tgattgtatc attagcggca ggtagttcgc caacagcttc
     2041 attaagttta acaagttcga ttcaaacaat gactggatat attgttgaga tagcgacagg
     2101 tgatgcaaca tttggatcaa atatttatta cagtatttat gctgtagggt tcacactatt
     2161 tatctttacc ttaatcatga atttactttc tcagtggatt tctaagcgtt ttagggagga
     2221 gtattaatat ggaaacgaca gataataata gacaatcact cgtcgatcaa caacttgtcc
     2281 aaaaacattt atcatccaga acggttaaaa ataaagtgtt caaactcata tttttagcat
     2341 gtacattatt aggacttgtc gtacttattg cgttgttaac tcaaacattg attaaagggg
     2401 taagtcattt aaatttacag tttttcacta atttttcttc ttcaacacca tctatggctg
     2461 gcgttaaagg cgcgttaatc ggttcacttt ggttaatgtt aagtatcatt ccattatcaa
     2521 tcatcctagg aataggtaca gctatatact tagaagaata tgcgaaaaac aacaaattta
     2581 ctcagtttgt taaaatcagt atttccaatt tagctggtgt accatcagtt gtatttgggt
     2641 tattaggtta tactttgttc gttggtggtg cagggattga agccttgaaa atgggtaaca
     2701 gtatattggc agcagcgcta acaatgacct tactgatatt accaattatt attgtttcaa
     2761 gtcaggaagc aattagagct gtacctaact cagtacgcga agcttcttac ggcttaggtg
     2821 ctaataaatg gcaaacgata agacgtgttg tcttaccagc agcgttacct ggtattttaa
     2881 ctggattcat tttgtctctt tcaagagcac tgggagaaac agcgccactt gtgctaatcg
     2941 gtataccgac tatattattg gcaacaccta gaagtatatt ggatcaattt tcagcattac
     3001 ctatccaaat atttacttgg gcgaaaatgc ctcaagaaga attccagaat gttgcatcgg
     3061 caggcattat cgttttacta gttatcttaa tcttaatgaa tggcgttgcg attattttac
     3121 gtaacaaatt tagtaaaaaa ttctaattta aacaatcaat ctcatttatc tattaaaaag
     3181 ggagttttaa atatggcgca aacacttgca caaactaaac aaatatctca aagtcatacg
     3241 tttgatgtct cacaaagtca tcataaaaca ccagatgata caaactcaca ttctgttata
     3301 tattcaacac aaaatttaga cttatggtat ggcgaaaatc atgcattaca aaatattaat
     3361 ttagatattt atgaaaacca aattactgcc attataggtc catctggttg tggcaaatca
     3421 acatatatta aaactttgaa tcgaatggtt gagttagtac cttctgtgaa aacagctggt
     3481 aaaatattat atcgagatca agacattttt gatcaaaaat attctaaaga acaattacgt
     3541 acaaatgtgg gcatggtctt tcaacaacct aatccatttc caaaatcaat atacgataat
     3601 attacttacg gtccaaagat tcacggtatt aaaaataaaa aagttcttga tgaaatcgtt
     3661 gagaaatcat tacgtggcgc tgcaatttgg gatgaattaa aggataggtt gcacacaaat
     3721 gcatatagtt tatccggtgg gcaacaacaa cgtgtttgta tcgcgcgttg tttagcaatt
     3781 gaacctgaag tcattttaat ggatgaaccg acatcagcat tagatccaat ctcaacatta
     3841 agagtagaag agttggttca agaactaaaa gaaaagtata caattattat ggttacacat
     3901 aatatgcaac aagcagctcg tgtatcagat aaaactgcat ttttcttaaa tggttatgtc
     3961 aatgaatatg atgatactga taaaattttc tctaacccat caaacaagaa aacagaagat
     4021 tatatttcag gaaggtttgg ttgatatata atggcaataa ttagacaacg atatcaggag
     4081 caacttgatg atttaataaa agaattacgt cggttaggtg caaatgtcta tgtgagtatt
     4141 gaaaatggta taaaatcatt aagtattgac gatagaggct ttgcacgaca aacagttaaa
     4201 aacgataaac atatcaatca attaaattat gatattaatg agcgagttat catgttaatt
     4261 acaaagcaac agcccattgc gagtgatttg cgtatgatga tttcttcatt aaaaatcgcc
     4321 tccgatttag aaagaatagg agataatgcc tcgagtattg ccaatattcg attgcgtaca
     4381 aagattacag atgattatgt gttaacccgt ttaaagacaa tgggtaaatt agctatgtta
     4441 atgttaaagg acttagatca agcatttaaa aagaaagata ccgtattaat aagagaaata
     4501 attgagcgtg atgaagatat cgatgactta tatagtcata ttattaacgc aacgtatctt
     4561 attgataacg atccatttgt cgctgcacaa gctcatttag cagcaagaca tttagaacgt
     4621 attggtgatc atattattaa catcgctgaa agtgtttatt tttatttaac aggtacacat
     4681 tacgaacaat aacttaaagt tattactata aaatccctta cgataaatat atatttctat
     4741 tattcataaa ccctcaaaaa aaccaagatt ctcacaatta gtaatgtgaa aatcttggtt
     4801 tatattgttc tactataaat tgtctcgcat cttagttatt tgcttgctca atttcatctg
     4861 ttaatttttc aacttcatcg actaaatcag aaatatattg aattgtagat ttaagtggct
     4921 gttctgtagt aatgtctaca cctgcaatgt ttgcaagttc gacaggtgat acactaccac
     4981 cttttttcaa tgtttctaac caagcatcaa cagctggttg gccttcattt ttaatctttt
     5041 gagaaacgac agttccgatt gttaagccag cagaatacgt atacgaatat aatcccatat
     5101 agtaatgagg ttgacgcatc catgttaatt cagcaccctc agtcatgtct actgcatctc
     5161 caaaaaattg tttataaaca tttagcatta tttcatttaa tgtcggcgca tttaaagatt
     5221 caccttgatc tactttgtga tacacttcac gttgataagc agcttctaat aaatgggtaa
     5281 ccatattatg ataatatgtt ctagataaaa ttgagccaat aacccaacgc ttaaatcttg
     5341 gattatcact tgtgttaaat aaataattgg ccatcaacat ttcattcatt gtagaagggg
     5401 cttcaacaaa gtacattgat gcttctgatt caagatatgg ttgatgtttt tgagctaatg
     5461 taaaatgacc tgcatgacct aattcatgtg ctaagacaaa tgcttcagcc attttaccag
     5521 tccaagaaat aaacacatat gaatgtgtaa agtatggact tgcacaaaat gcgcctgtat
     5581 ctttaccttt attttgtgca aaatcaatcc atcgctgatc gtatgcttca cgtaacatgt
     5641 ttgtatagtc atcacctaaa acacttaacg caccgaaaat ataatttttt gagtcttcaa
     5701 ttgaaatctc tggttcataa tcagggtcta cagaaatctt caagtcttca aaacgcatgt
     5761 tatctaatcc gtgaatacgt tgtaaaagtt tagcatattt ctgcataact ggtgccaagt
     5821 cacgcataat catatcgatt tgacggtcaa acatatcacg cgttacttct tgactatgta
     5881 ataaataatc gatgactgat tcaaatccac gtaaatcagc ttcaattttt tcttgttgta
     5941 cttgcatatt atatgtagcc gcggtagtat gctgatattt tcgaatccca tcgctaaacg
     6001 atttgaaact tttacgtcta aactcaggat ctttattatc ttcataatca ttttcaaacg
     6061 tagcatagtc gacagggtac gttgtaccat catgttcaaa tgaatcgaat gtaatatcta
     6121 gcatttttgt cgtgccgtat aaatcgtaag gactgtttag cgtaggtgat aaagttgcta
     6181 atacttgttc taccgacgca gataattgga atggcttttg ttttattaac tgtttaatat
     6241 agtgttgata tggacatgat tcttctaatt gttgaagtat ttcttctggt aattcaagta
     6301 tttctgactc tacaaatgat aattggctaa caattttacc gtatgtagta gataatttag
     6361 cgctcaatac ttgtgcctcg atattactag tatctacact taaacgtagt tctgcataat
     6421 tacttaagcg atctaaggca attaaaatat tttctaattc agctaaagca gtattaattt
     6481 gttcgattga atttaatgtt gttgcatatg tatgatgaaa ttgatttgct tgttgtacta
     6541 gagcgtcaat actttcataa tattgttgat catctttaaa taagtctact aaatcccatg
     6601 tttctgaaac aggaacatct tctcttaaag gtaaaccttg agacatataa tcactccttt
     6661 ggtataatac attcattata cataattttg tctcctaaac gataatgtta cgatttccat
     6721 gtcgcaagga acaatgattt ttctattagt agatagaata ttgcttgaaa ctgactgcta
     6781 ttgtttaagt ttttaaaaat ttataataaa atgtatacaa ggaaagtaat gaaa
//
LOCUS       DAKTGS010000143         6657 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.144, whole genome shotgun sequence.
ACCESSION   DAKTGS010000143 DAKTGS010000000
VERSION     DAKTGS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.144"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            46..606
                     /locus_tag="O0286_002426"
     CDS             46..606
                     /locus_tag="O0286_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCY0170769.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            625..3027
                     /gene="mnhA2"
                     /locus_tag="O0286_002427"
     CDS             625..3027
                     /gene="mnhA2"
                     /locus_tag="O0286_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCY0170770.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            3014..3439
                     /gene="mnhB2"
                     /locus_tag="O0286_002428"
     CDS             3014..3439
                     /gene="mnhB2"
                     /locus_tag="O0286_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCY0170771.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            3436..3780
                     /gene="mnhC2"
                     /locus_tag="O0286_002429"
     CDS             3436..3780
                     /gene="mnhC2"
                     /locus_tag="O0286_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCY0170772.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            3770..5266
                     /gene="mnhD2"
                     /locus_tag="O0286_002430"
     CDS             3770..5266
                     /gene="mnhD2"
                     /locus_tag="O0286_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCY0170773.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            5267..5749
                     /gene="mnhE2"
                     /locus_tag="O0286_002431"
     CDS             5267..5749
                     /gene="mnhE2"
                     /locus_tag="O0286_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCY0170774.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            5746..6048
                     /gene="mnhF2"
                     /locus_tag="O0286_002432"
     CDS             5746..6048
                     /gene="mnhF2"
                     /locus_tag="O0286_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCY0170775.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            6023..6460
                     /gene="mnhG2"
                     /locus_tag="O0286_002433"
     CDS             6023..6460
                     /gene="mnhG2"
                     /locus_tag="O0286_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCY0170776.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
BASE COUNT         2099 a          922 c         1188 g         2448 t
ORIGIN      
        1 aatatagcta tgattaagac acttaaagca aggggaggtt gagtaatgaa taaagtagaa
       61 gcgattaaat ttaatgatga tattgttaaa atgtatgaag cgctcaagat aaaatctgaa
      121 cgtgactatt tattctttaa gttagctata catagtggat tgaaagtatc agaattatta
      181 acaattacag tctctcaagt taagagacta attgaaaagt gtacgttatc agaaatgtgt
      241 aaagcacatt ttcattcgtt gattaaaatt aggttaccag aaacattatc gaaagaacta
      301 cttcaatata tagaggacag gagtctttcg aatgaagacg ttctttttca atcactacga
      361 acaaatcaag tattatctag acagcaagca tatcgaataa ttcaccaagc atcaattgaa
      421 gctggtatag ataatgtagg actaacgaca ttgcgtaaga catttgcata tcatgcttat
      481 caaaaaggta tacctatacc agtcattcaa aagtatttag ggcatcaatc tgctattgaa
      541 acactaaatt ttatcggttt agaaaatgag tgtgaacata gtatttatat ttcattacaa
      601 ttatagaaac aaaggaggct aataatgagt ttggtttatt tattaattgc tatacttgtg
      661 attatggcga tgatacttct aatgtctaaa cgtagagcat tggctaaata tgccgggtac
      721 atagcgttgg ttgcacctgt aatttcatct atctattttt tgattcaaat accatcagta
      781 gctaaactgc aatatctttc tacctctatt ccatggatta agacattaga tattaattta
      841 gatttacgtt tagatggttt aagtttaatg ttttctctta ttatttcact tattggaatt
      901 gcagtattct tctatgcaac tcaatattta tcctctcgaa aagacaattt accaaggttt
      961 tatttttatt taacgttatt tatgttcagt atgattggta ttgtattatc agacaatacg
     1021 atattgatgt acattttttg ggaattaacg agtgtatcat catttttatt gatttcatat
     1081 tggtataaca atggtgacag tcaatttggt gcgattcaat catttatgat tacagtattt
     1141 ggtggattgg cgttattagt tggttttatt atgctgtata tcatgacagg aacgaataac
     1201 atcacagaga tattaggaca agcagatcat attaagaatc atggattgtt tatccctatg
     1261 atttttatgt ttttattagg tgcatttaca aaatcagcac aatttccatt tcatatttgg
     1321 ctacctagag caatggctgc acctacacct gtaagtgctt atttacattc agccacgatg
     1381 gtaaaagctg gtatcttttt attacttcga tttacaccat tattaggtct tagcaatatg
     1441 tacgtatata tcgttacgtt tgttggttta ataacaatgt tatttggttc aattacagct
     1501 ttaaaacaat gggatttaaa aggtatccta gcgtactcta caatcagtca acttgggatg
     1561 attatggcta tggtgggtat aggtggcgga tatgctcaac accaacaaga cgcaatagca
     1621 tctatttatg tatttgtatt atttggtgcg ctatttcatc taatgaatca tgccatcttt
     1681 aaatgtgcgc ttttcatggg agtaggtatt ttagatcatg aagcaggttc aagggatata
     1741 cgaattttaa gtggaatgcg tcaactattt cctaaaatga atctagtcat gacgatagcg
     1801 gctctatcta tggctggagt accattttta aatggatttt taagtaaaga aatgttttta
     1861 gatgcattaa cacaaactgg acaattatcc caatttagtt tgatttcaat gatagctatc
     1921 gtgtttgttg gtgttattgc gagtgttttt acattcacat atgcactata catggtaaaa
     1981 gaagtatttt ggacaaaata tgattctaag gtttttacta aaaaaaatat ccacgaacca
     2041 tggttgttta gtttaccatc tcttatatta atggtgctag tacctgtaat cttttttgta
     2101 ccaaatatat ttgggaaggg gattatcgtt ctagcattaa gagctgtatc aggtggtaat
     2161 catcaaattg atcaattggc accacatgtt tcgcaatggc atggatttaa cataccgctt
     2221 cttttaacca tcatcattat tttattgggt agtgtactag caatcaaagt agattggaaa
     2281 aaagtgttca caggtaaaat tagacagatt tcagtttcaa aaagctatga gatggtatat
     2341 cgacattttg aaaagtttgc tacgaagcga tttaaacgtg ttatgcaaga tcgtttaaac
     2401 caatacatta ttatgacctt aggcatattt atgattatca ttggatatgg ttatattcga
     2461 attggacttc ctaaagtaca tcagttacat gtttctgaat ttggggcatt agaaattata
     2521 ttagcaatcg taactgtcac aattggtatt tctttaattt ttatacgtca acgactgaca
     2581 atggtcattt taaatggagt catcggattt gttgtgacct tattctttat agcaatgaaa
     2641 gcccctgatc tagcattgac tcagctagta gttgaaacaa taacgacgat actatttatt
     2701 gtcagttttt caagattacc aaacgtgcca agatctaacg ctaacaaaaa aagagaaata
     2761 attaaaattt ctgtatcact cttgatggca cttattgttg tatcattaat ttttattaca
     2821 caacaaacag atggtttatc atcaatatca gacttttatt taaaagctga caaactaaca
     2881 ggtggtaaaa atattgtaaa tgcgatactt ggtgacttta gagcattaga tacattattt
     2941 gaaggattag tgttaattat tactgggcta ggtatttaca cattattaaa ttatcaagat
     3001 cggaggggac aagatgaaag agaatgatgt cgtgttaaga acggtcacga aacttgttgt
     3061 atttatttta ttgactttcg gattctatgt cttcttcgca ggtcataata atcctggtgg
     3121 tgggtttatt ggtggtttaa tatttagttc agcgtttatt ttaatgtttc tggcttttaa
     3181 tgttgaagag gttttagaaa gtttaccgat tgattttaga attttaatga ttattggagc
     3241 attggtatca tctattactg cgataatacc tatgtttttt ggaaaaccat ttttgtctca
     3301 atatgaaaca acttggatac ttccaatttt aggacaaatt catgtaagta caataacact
     3361 ttttgaatta ggtattttat tctcagttgt tggtgttatt gtcacagtga tgttgtcgct
     3421 tagcggaggt cgatcatgaa tttaatatta ttactagtta taggattttt agtgtttata
     3481 ggaacatata tgattttatc aatcaattta attcgtattg taatcggaat ttcaatatat
     3541 actcatgctg gtaatctcat tattatgagt atgggaacgt atggttctag tagatcagaa
     3601 ccactaataa ctggtggaaa ccaattgttt gttgatccct tgttacaagc tattgtacta
     3661 actgcaatag ttatagggtt tgggatgact gcgtttttac ttgtacttgt ttatagaact
     3721 tataaagtaa caaaagaaga tgaaattgaa ggcctaaggg gggaagatga tgctaagtaa
     3781 cttattgatt ttaccaatgt tattaccatt cctttgtgcc ttaatccttg tatttttaaa
     3841 aaataatgat cgtatttcta aatatttata cttaggtaca atgactatca ccacaattat
     3901 ttcattaatg ctattaattt atgttcagcg tcaccgtcca attacgctag actttggagg
     3961 atggtcagcg ccctttggta tacagttttt aggagattct ttaagtttaa ttatggttac
     4021 aaccgcttcg tttgtgatta ctttaattat ggcatacgga tttgggcgtg gcgaacataa
     4081 agcaaatcgt tatcacttgc catcgttcat attattttta agtgttggcg tgataggctc
     4141 ttttctaaca tcagatttat ttaatttata cgtcatgttt gaaattatgt tactagcgtc
     4201 atttgtactc attacacttg gacaatctgt agaacaatta cgtgctgcaa ttatttatgt
     4261 tgtcttgaat attattggtt catggctatt cttattaggt ataggtttac tttataaaac
     4321 agtaggtaca ttaaactttt cacatattgc aatgcgtttg aatgacatgg gagataatcg
     4381 cactgttaca atgatttcat taatcttctt agtcgcattt agtgcgaaag cagcgctggt
     4441 cctttttatg tggctaccca aagcctacgc tgtgttaaat actgagcttg cagcattatt
     4501 tgcagcgtta atgaccaaag taggggccta tgcattaatt cgattcttca ctttactatt
     4561 tgatcaacat aatgatctca tacatccatt gctagcaact atggctgcta taactatggt
     4621 catcggcgct ataggtgtca ttgcttataa agatattaaa aagattgcag cttaccaagt
     4681 cataatctca ataggattta tcattttagg tttaggaaca aacacgtttg caggtattaa
     4741 tggtgcaata ttttatttgg taaatgacat tgttgtaaaa acattgctat tttttattat
     4801 tggtagttta gtttacatta caggctatcg acaatatcaa tatttgaatg gcttagctaa
     4861 aaaagaacct ttatttggag ttgcgtttat tataatgatt tttgctattg gcggcgtgcc
     4921 tccatttagt ggctttccgg ggaaagtact tattttccaa ggtgcattgc aaaatggcaa
     4981 ttatattgga ctagcgttaa tgattattac tagtctaatt gcaatgtaca gtttatttag
     5041 gatacttttt tatatgtatt ttggagataa agatggggag gaagttaatt ttaagaaaat
     5101 cccgctatat cgaaaaagaa ttttaagtat tttagtagtt gtggttatcg caatcggaat
     5161 tgctgcacct gttgtgttaa atgttacaag tgatgcaact gagttgaaca cgagtgatca
     5221 attatatcaa aaacttgtaa atccgcattt gaaaggagag gactaaatga atcaaatagt
     5281 tttaaatatt atcattgcat tcttatgggt attatttcaa gatgaagatc attttaaatt
     5341 ctcgactttc ttttctggat atctaattgg tttaattgtc atttatatat tacacaggtt
     5401 tttcagcgat gatttttatg ttagaaaaat atgggtagct attaaatttt taggtgttta
     5461 tttatatcaa ttaataacat ctagcattag cacgattaat tatattcttt ttaaaacaaa
     5521 agatatgaac cctggattac tttcatatga aacaagacta acaagtgatt ggtcaataac
     5581 atttttaaca attttaatta ttataactcc agggtctaca gtaatacgaa tttctcaaga
     5641 ctctaaaaag ttttttattc atagtatcga cgtgtcagaa aaagaaaaag atagtttgtt
     5701 aagaagtatt aagcattatg aagacttaat attggaggtg tcgcgatgat acaaacaata
     5761 acacatatta tgattattag ttcactcatt atttttggaa ttgcattaat catctgttta
     5821 tttagattaa tcaagggacc tacaacagca gatcgtgtcg ttacatttga tacaacaagt
     5881 gctgtcgtaa tgtcaattgt gggtgtgtta agtgtactta tgggcaccgt ttctttctta
     5941 gattcaatca tgctcattgc cattatatct tttgtaagtt ctgtttcaat atcacgcttt
     6001 attggtgggg ggcatgtgtt taatggaaat aacaaaagaa atctttagtc ttattgctgc
     6061 tgtgatgttg ttgttaggta gttttattgc tcttattagt gcaataggta tcgtgaaatt
     6121 ccaagatgtt ttcttaagaa gtcacgctgc gacaaaaagt tcaactttat ccgtgttatt
     6181 aactttaatc ggtgtattaa tttattttat tgtgaataca ggatttttca gtgtgcgttt
     6241 attactgtca cttgttttta ttaatttaac ttcaccagtc ggcatgcact tagtcgctcg
     6301 cgctgcttat cgcaacggcg cttatatgta tcgaaaaaat gatgctcaca cacatgcatc
     6361 aatattatta agttcaaatg aacaaaactc tacagaagca ttacaattac gtgctgaaaa
     6421 acgagaagag catcgtaaga aatggtatca aaacgattga agtgttggct aaattgttaa
     6481 gcataacaat gcttttgaaa atctgttttc aaaattaatc attaaaagag tactttgaaa
     6541 ttggttagtc gattttaagg tgctcatttt ttgtattata agttaatgtt gttatgtgaa
     6601 agcaagttgt ttatttatac aaacaattag tgataaaata aaattaaata attgcaa
//
LOCUS       DAKTGS010000144         6656 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.145, whole genome shotgun sequence.
ACCESSION   DAKTGS010000144 DAKTGS010000000
VERSION     DAKTGS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.145"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(78..269)
                     /locus_tag="O0286_002434"
     CDS             complement(78..269)
                     /locus_tag="O0286_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0170777.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            430..759
                     /locus_tag="O0286_002435"
     CDS             430..759
                     /locus_tag="O0286_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0170778.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            772..1233
                     /locus_tag="O0286_002436"
     CDS             772..1233
                     /locus_tag="O0286_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0170779.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1251..1685
                     /locus_tag="O0286_002437"
     CDS             1251..1685
                     /locus_tag="O0286_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170780.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            1714..2109
                     /locus_tag="O0286_002438"
     CDS             1714..2109
                     /locus_tag="O0286_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170781.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            2199..2345
                     /locus_tag="O0286_002439"
     CDS             2199..2345
                     /locus_tag="O0286_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170782.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2342..2956)
                     /locus_tag="O0286_002440"
     CDS             complement(2342..2956)
                     /locus_tag="O0286_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCY0170783.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            3082..4287
                     /locus_tag="O0286_002441"
     CDS             3082..4287
                     /locus_tag="O0286_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0170784.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            complement(4330..6356)
                     /locus_tag="O0286_002442"
                     /pseudo
     CDS             complement(4330..6356)
                     /locus_tag="O0286_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6349..>6656)
                     /locus_tag="O0286_002443"
     CDS             complement(6349..>6656)
                     /locus_tag="O0286_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCY0170785.1"
                     /translation="YEPLIRKNDKEFKEGMERARKEVNYAANTDAVATLFSTKKNFTK
                     DNTVDDVIELSDKLYNLKNKPDKSTITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
BASE COUNT         2461 a         1059 c          911 g         2225 t
ORIGIN      
        1 ttcctccttt taagttgttt atgtccattt gttacattac ctaaaagtta taaaaagtta
       61 tacgaaggtc aaaaaattta gacctttatc tggtctacct caatatcata taactttgct
      121 aaagcgtaaa ctgtaacatt acttaaatta gtgtcatcct tttcccaatg accaactgtc
      181 tttgcattga cgcccaatat atctgctact tcttgctgtt tcaagtccct tacaagtctc
      241 cattttctta cggataattg ttccggcatg tcgtccacct ccctttcaca cgtataactt
      301 tatattactt tgtattactt gtcaacagat aaagttatat ttcttcataa aaagttataa
      361 aaagtattgt aaagtaatgt cggttatggt aagttaatat tacattaagt aatattaaag
      421 gagacaacta tggagaataa taaagtcaga aaaattttat ctgaaaacct tcaagaactt
      481 atgaatgata aaaatattga tcagagagaa cttgctgaag ctattggagt ttctcaacct
      541 acagtctcca attggattca acaaactaaa tatccacgaa ttaaaagaat tcaacaactt
      601 gcagattact tcaatgtacc gaaatcaaga attactgaat caaaaaaaga tatacatcaa
      661 gaaacaattg ctgctcattt tgataaggag ggattaactg aagaagagat tgaagaagta
      721 aatagattca ttgaatgggt tagaaataga gacaaataaa gggtgtttat attgggatta
      781 tacgaagaac tttgcataaa taacgaaaaa ataaagatag aagaaactga ccagcttcca
      841 aatttccaac ctggatgcta tatgaacgga aaaatttata taaggcgtaa tttatcagaa
      901 gtacgtaaag cagaagtgtt atatgagcaa cttgcccacc acaagttgac gtatggcaac
      961 attttagacc aaacaaaatg gattaataga aaatttgaaa attatgcacg tagacatggt
     1021 tttacttcag ctgtaccgct acatgaaatt gtagaagctc acaactacgg tgttcgtaat
     1081 ttgtacgagt tgtcagagta tctgcagttg agtgaatcat acatactaga agctatagaa
     1141 caatataaaa agatatatgg tattggaact cactatggcg agtattctat tacgtttgag
     1201 ccgttgagag tttttaaata taaggaaata taaacaaagg agagatacat atgaaaaaag
     1261 taatcggact gctactagta agtacattag ctttaacagc ttgtggtgaa aaagaaaaac
     1321 caaaaaaaga agaaaataaa aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa
     1381 cgcaacaaga aaaaatgaaa aaagttgaag ataaaaatcc acctaataat agcatacaaa
     1441 ataattcaaa caatcaaaac caatcacaaa acaatcaact taataataat tcagatccat
     1501 ctaataatac tcctgcaaat ataaatgaaa acgattcaca aaatactaat ttaaatgatg
     1561 agtatgtcgt ttcgcctggc tggactaaag atgaacaggc taaagctttt gaagagtaca
     1621 aaaaaggaaa agaagaggaa gcaagagctg gtgctagcgc agtaccagga gccaatatta
     1681 actaataaaa caatataaga aagaagagct aatatggaaa caaataaaac aatcgattta
     1741 atgaattatg tggaatttcc aaaaagatac acagaggcaa aaggcaaatt agttgcacaa
     1801 ccaataacta ctataaatag cgcaagaaga gttgaaaatg aagatatgac tgtttgctac
     1861 attttagatc aggatgatga tgtaatggac tttatctttg acagagatat aattactgtt
     1921 tactgtcctg aaaacggaac tgcgactgat gaatattttt gtgaaattat atttaactca
     1981 gatgacacat ttaccctaaa gcgattatct aattacgtta ccattaaaga tagaagctac
     2041 ccaatgtcaa aaataaatga cgtaaacatt acgggcaaag tcgtcagatt atttagagat
     2101 tttaaataaa cttggcttta attacgatta aaagtaccta tatagcgtga cgagaaaaag
     2161 gattaaaaaa aatcaaaaaa cgcctactag tgtagacgtt gaatggtggt gagaatttta
     2221 tggtagataa aaacaaaaaa caagaaacta ctcgtagtaa cccattaaac aaaagttttg
     2281 aaaagtcagg cgccagcgaa aaattaaaaa gcactttatc agaaaaagct aagaaaaaag
     2341 attagtattc attcattaaa tataaatcca atttaatttg ttgtttaagg tctacaagcg
     2401 tatgtttaat atacaattca tcgtttgacg gtaaatcaga tactttgaaa tcttgtcgct
     2461 caacttctag taaatcgaaa tcgctaccag ctgaattata ggttttaagt tcaccctctt
     2521 caatgattct gttttcaaag tcttttataa ctataaatac tggtttaccg ttgttattaa
     2581 acaacttgtc tcttttgtct aataagctta tacaatccaa tttcataaac tttctggtta
     2641 tattaattaa ccagataata aatttaacaa ttaaaggatt aaatacaaac actgttaaaa
     2701 caaaaataaa tagaaacaaa atatttgctt ttagacctgt aagcaactga attaaattca
     2761 aattttttaa atcaacatta ttaaaaatta taaaactata aaaccatatc aaacatgttt
     2821 caatagaaaa aatcaataat acaggagtat tgataacctt gtttttttca ctaactaaac
     2881 ctatcattgt tagatattta tatggtatgt aacctaaaac tcctgtaaga agaagcgccc
     2941 ctagaaattg agtcatctta tcacctactt tttattttat tataacacat ttagtaccta
     3001 gtactaaatt acgggtagcc cgactaccct tattattttt taatatttta tagaacatac
     3061 gttcttgcag gaggtataaa catgtggatt gaaaaattta aaaacaaaaa taacgaaact
     3121 aaatacagat actacgagaa gtataaagat ccatacacag ataaatggaa gcgcgtaagt
     3181 gttgtgttga acaagaatac aaaacaatct caaaaagaag caatgtttcg tttagaagaa
     3241 aaaataaaag aaaaactgaa caacaagtcg tcaagcgaat taaaaacttt gacttttcac
     3301 gcgctattag atgaatggct tgaatatcat ataaaaacat caggttcaaa gttgactact
     3361 cttaataata taaaaataag aattagaaac attaaacgat acagctctga gaacttgctt
     3421 ttaaacaaac tagatacaaa atatatgcag atatttatta ataaattatc agatatctat
     3481 tctcaaaatc aagtaacccg tcaactcgga gatatgaaag gagctattaa atatgcagtt
     3541 aaattttaca attatccaaa tgaatatttg ttaactaatg tcaaaattcc taaaagaaga
     3601 aaaacaatag aggatatcga aaaagatgaa tctaaaatgt acaactattt agaaatgaac
     3661 caagtcctac agatacgtga tcatatacta aatgataata agttacacaa gcgaaatcgc
     3721 attttaattg ccagcatctt agaagtacag gctttaactg gtatgcgcat aggagaacta
     3781 caagcactgc aggaaaaaga tatagattta ttaaacaaaa ctatcaatat aacaggtaca
     3841 attcaccgca ttaaatacga ggaaggattc ggatacaaag acactacaaa gactataagt
     3901 tcaaaaagaa gtatcagcat caattctaga accgtagaaa tttttaaaaa gataatactg
     3961 gaaaacaaaa tgttgaaaag atggaattcg agctatgttg acagagggtt catattcaca
     4021 acaaaaaaag ggaatccttt atgtaataat caaatcgccg gtgtgcttaa gaaaactaca
     4081 aaagctttaa atatgaataa gaaagttacc acgcacacat ttagacatac acacataact
     4141 ttattagtag aaatgaatgt ttctttaaaa gcaattatga aaagggtagg acatgtagat
     4201 gaaaaaacaa ccattcgcat atatactcat gtaactgaaa aaatggatag agaactaact
     4261 caaaaactcg aaaacattcc aagttagctt aaatccgccc tttttttgcc cttatatttt
     4321 ttacaagctt tataaaacgc ttgagaacac tggcgttaaa gcttttcttg aaataaacat
     4381 atcatcataa tgtgatggtt caaatatcat ttgtacaatc aaaggcttca tgttcttaac
     4441 aatatcatct aaatggttat ctaaaattgg tgacactgct tttaaatcat taagaaaagg
     4501 ctcccatttg cctaaagtat tatctaattc ttctaattta gttttaatat aattacaagt
     4561 tacattagga atcagggaca aaaattcttt cttttttaca tttaacattt caattgcatg
     4621 tcttaaattc ttacgtattt tgggaattgt attaatcaaa tattttatta catcgacaat
     4681 tttcgatgca tattcatcat atataccttg aacatagtct gctatttttt taataccatc
     4741 atcgatatgg tcttttaata ttttcatttt tcttcctaaa taattagaag gtataactag
     4801 accctgtacc atattttcgc cgctacaatt aatttgaaaa tttccatcta aaattgttgc
     4861 atcttgttgt ttcataatac ttctaatatc tgcaatttgc ctaccataaa tatcattttg
     4921 attttttatt tggtctatat tctgtttcac tactttcaaa tgtttcatca tttcttcaga
     4981 tactccatct ctgaagtcgt gatctatatt tttgaaaatt tctaaaattt cattatctat
     5041 actatcatac actttttcta taaaagattt tataccttta aacaactcat taattctttc
     5101 ttttaatgca tccaatgcaa aatcaggtaa taagtgttta acagcactaa tactttctat
     5161 tgtttcatct gcaacttctt caagtgagtt tattttacta attaaagttc tttccatttc
     5221 ttctaactga aataagttaa tcttatcctt aaatccttct gataattgtt tctttctatc
     5281 tgcaaaattt ttattttcat tttctgagat gttaaaactt tcatttaaaa agattacgca
     5341 ttctgctaac ataccacttg tttcaccagt aatcagttta ctcaacgcat caagattttc
     5401 taaattaagt ttaattaaag ttcctcttcc agaacgtgca atcgaatctc ctgtccaaac
     5461 atttattgga attcgcccat ccatatctaa tgttatgtta atagtctttt ttactttttt
     5521 ccatttttaa tttctgtatc ttttaccgac tttattttga ttagtggtac agtatcgtat
     5581 gtgttatctt ttctatttaa cttcctttta taacctacat ggctgtctat taaagtatct
     5641 aacctgggca caccatcatt aatgttaacg cgttttcctg gcatatcttt gatgaatgga
     5701 tcttgtaacc atgttaataa atcgttggta ctattaaaac taatcatatt atcaaagcgt
     5761 ggtctagcaa atttctgcca agcagcataa ggaatcattg ctgagtcagt agcaacaact
     5821 ttttcattcg gatgtttcgc tccttgatat tttgctcctg caccgccttc cgaattaccg
     5881 ccatccgcca caatggtttt gtttttgaag ttacttgatt ccattctata tttttttaaa
     5941 aaatcactat ttgataatct atctgcatct tctaacttta ttttatattg atttgataat
     6001 tgatctgttt gctttaaata atctgtgctt tcattatcat tattcattaa tttagcattt
     6061 tggagccaat catctccaaa ccccgatgat tttaatggat tatttggatt aattgcctca
     6121 ttagatgttc cttgataaat tattgtctgt tgaccagttg gtttactttt atcatctaat
     6181 aactcatata ttttaatatc agcagcacca ttcaaattac tattcttatc atcattataa
     6241 ctgtctactt gtttaaacct ttttccatta actttaaatt ctttttttat atcaatattt
     6301 tgataaaccc agtaactact taattctgtt aaatccctat cattaatttt attcatcttt
     6361 actgtgcacc ccatattcta ttggacgatt atcatcataa aaggctttct tagtattaat
     6421 agtgggtttc cctatttgta ttgtgattgt agatttatct ggtttatttt ttaaattata
     6481 taatttatca cttagttcga ttacatcatc tactgtattg tctttagtaa agtttttctt
     6541 agtagaaaaa agtgtagcaa cagcatctgt atttgcagcg taattcactt cttttcttgc
     6601 tcgttccatt ccttctttaa attctttatc attttttcga atcagtggtt cataat
//
LOCUS       DAKTGS010000145         6643 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.146, whole genome shotgun sequence.
ACCESSION   DAKTGS010000145 DAKTGS010000000
VERSION     DAKTGS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.146"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            97..825
                     /locus_tag="O0286_002444"
     CDS             97..825
                     /locus_tag="O0286_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCY0170786.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            910..1163
                     /locus_tag="O0286_002445"
                     /pseudo
     CDS             910..1163
                     /locus_tag="O0286_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1179..1502
                     /locus_tag="O0286_002446"
     CDS             1179..1502
                     /locus_tag="O0286_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170787.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            complement(2372..2733)
                     /gene="ssrA"
                     /locus_tag="O0286_002447"
     tmRNA           complement(2372..2733)
                     /gene="ssrA"
                     /locus_tag="O0286_002447"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2824..3288)
                     /gene="smpB"
                     /locus_tag="O0286_002448"
     CDS             complement(2824..3288)
                     /gene="smpB"
                     /locus_tag="O0286_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCY0170788.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(3310..5682)
                     /gene="rnr"
                     /locus_tag="O0286_002449"
     CDS             complement(3310..5682)
                     /gene="rnr"
                     /locus_tag="O0286_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCY0170789.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(5716..6456)
                     /locus_tag="O0286_002450"
     CDS             complement(5716..6456)
                     /locus_tag="O0286_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0170790.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            complement(6572..>6643)
                     /locus_tag="O0286_002451"
     CDS             complement(6572..>6643)
                     /locus_tag="O0286_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCY0170791.1"
                     /translation="NRLTIILSILFFVLMICISYLGM"
BASE COUNT         2289 a         1194 c          969 g         2191 t
ORIGIN      
        1 aatctcacaa aatatcaact ttgtttaatt actcaaaatg ataagtcatt tagagttttt
       61 aaaataaatt tttgcgaaat aaaggagaca tgttaaatga aaaaagtaat ggggatatta
      121 ttagcaagta cacttatctt aggtgcttgt ggacatcatc aagatagtgc aaaaaaagag
      181 agcactagtc acaaaaagaa agaaaatgac aatgaagaat taaatgaaga acttaaagaa
      241 tttaaaagca aaaaaaatat ggatataaaa attaaaggcg atactattgt tagtgacaaa
      301 tttgaagcta aaataaaaga accgtttatc atcaatgaaa aagatgagaa aaagaaatat
      361 atcgctttta aaatggaaat tactgctaaa aaagacgata aagatttaaa tccatcttct
      421 atttctcatg actatattaa tatcactcaa gatgataaaa atacagtaaa taaattaaga
      481 gatggttatc ttttaagtga taaaaaatat aaagattgga cagaacataa ccaagatcaa
      541 attaaaaaag gcaaaactgc acaagccatg tttatctatg agttaagagg tgatggaaat
      601 attaatttaa atgtccataa atactcagaa gataaaacag ttgattctaa atcattcaaa
      661 tttagtaaac ttaaaaccga agatttttct catagagcgg aaacaagaga agaagtagaa
      721 aagaaagaaa aagaatttga agaagagtac aaaaaagaac aagaacgaga gaaagaaaaa
      781 gaaaagcaaa aagatgacga ccacagtggt ttagatgaag tataaatttg gtttgttgat
      841 tgtcttacat tcagttattt aatttttaga gaaactgact tatttatagc ttagccaaca
      901 tgatactata aagctatgat taaaaataaa aaatctgtgt ttaatggtgt taaagaaatc
      961 agtccccgtg ccggaaaatc ataaaaaaaa gatgggataa atttgtaaat cccgcattga
     1021 ataatgcatt aaaacaagaa gaggctacat ggggaaatgt acaaggtcaa gtttcacaag
     1081 cattgatggg aactggtatt aaagattcta ctgcaagaag catagggttt tgggtttccc
     1141 aagttggtca agcattaata taaaaagagt gtgataaaat gaataatgaa gaattagaaa
     1201 tgagattgtt attaatgaaa caatcaatag aacaattaca agaagaacta gcgcctaact
     1261 taaaaactag agatttagtg ttattaagat atatgtattc atataaagaa attaatatgc
     1321 tagattctta cttgttccaa ttggctacaa ataaagagca aataacaaaa aaacaattta
     1381 aaacaaaatt ggaaaacatt agagaagtac cagaaatacc tattagacaa gttaatgata
     1441 tattagaagg ttataaaaat agtgagttat atgttgagtt gataaatagc atcctaaaat
     1501 aaaaataaac atactaagat tagctatgaa ggaatctatg acgatagatt ttttcatagc
     1561 tattttttat agttataaag aggagtagtc tgttctgact cttggatttt aaatatgtat
     1621 aataaacaag taagctttac tctcgccttt taaaattcgt ttgtagtatg ttgggttctt
     1681 aaaatcgtga ataggaaaat gcaatgagaa aggttaagta aagtttttaa cttctcattt
     1741 attcaatgga aaatttttta tcgattactt agacattaat attagtaaaa gaagtagtgt
     1801 agttgcaaat tataaaaact aaaaattcca aaaagagttt atcatccaaa acaataaaaa
     1861 tggctacaat ggtatgttgt gaaatgcatt tcttaactac gacttctttt atttccgtct
     1921 cgtcaactat tgctgattgt gttaataggc atattgattc taatgaaaat atcaaaactc
     1981 ggctcatctc tttataacta cacccactta gtgacgtaac aaaaatgaaa agttcttttt
     2041 cagtagccat ttatccaatt aagtgtagac gattcacatt tttatattga catatataac
     2101 atggagaatt ttttgcgtat aaagaagttt cttcatgaac aatgttgaat gtaaaactca
     2161 gaatccaact tttttgacac ttatttgaca cacatcactg tccccttata tctcaaaaac
     2221 taattcataa tcacttatta ctctttcctc catcaaataa acacctatac aaacactgct
     2281 atatcaatac tcacaacaaa ttctgcaatg cattatcccc tgaaaaacac aaaataaaaa
     2341 agcccacaac cacaaaggct gtaggctaca aatatggaga cggcgggatt tgaacccgcg
     2401 tccaaaggtc ctgatacaca tctttctacg tgtgtagttt atcgataggt ttcgcatcat
     2461 gaaaagtgat aaacaaccaa catgatgcta gtttgattaa gtttcttcta aacagacttc
     2521 aaacggcagt gtttagcata tcctattaag gttgaatcgc gttaacagca cataggaaat
     2581 gctgttaggc gatgcagagt gcgattaggc agctactgcg aaattattgt ttgatttgcc
     2641 agttattata aactgtgtgt gttgatgacg aagacaaccc tccgacacgc ttaatgagct
     2701 cgggggaccc ctgtcgaatc catgaacgtc cccgaaataa taaatcaaac aaatcatact
     2761 tttgtaattt acaaaagcac atataactat atcaaatata gcccgattaa gcaactaaat
     2821 cgcttaataa cgggctttca tatcgcgcgc aacatctcgt ttgactgctt tttctttcaa
     2881 agcttgacgt ttatcatatt ttttcttacc tcgtgcaaca ccaagtaata ctttacaatg
     2941 tccatgcttc aaataaagct ttaacggcac aatcgaataa ccaatctcac gtgtttgatc
     3001 acccaattta atgatttcac gcttgtgcaa taataatttt cgagaacgaa gaggatcgtg
     3061 attaaaacga ttcccttctt cgtatggtgc tatatgcata ttattcaaat acatttcacc
     3121 gtttttaact tgcgcataac tatctttaag gttagcacta cctcggcgaa ttgattttat
     3181 ttctgtgcct tgcaatacaa ttcccgcttc aatcgtatct tcaatattat aatcatgtct
     3241 tgccttacga ttttccgcta atgtacctgg tgatttcttc ttagccattc atactcacct
     3301 cattgctgct tattttttct tacgacgtgc tttctttttc acacttttat ctttataaaa
     3361 tggcttatgc ttactattac ctgatttatc attattacgt tggtttttac ctttacgttg
     3421 cttacctttt ttcttacgac ccttatcatc agattttgat ttatctaatg atttaccacg
     3481 cgttttggct tgaattgtct tacctcgcgc tgggcgctgt gatcgatcat ttttcggtaa
     3541 aggcatacct acaatttgaa aatcaattaa tcgttcatct acatcaacat gcgtcacttt
     3601 aaccttaact gtgtcaccaa ttctaaatac tttagcttga cgctcaccaa ttaatgccat
     3661 ttgacgctct tcaaaacggt aataatcatc agtcatattc gcaatatgaa ccataccttc
     3721 tatcgtattt ggcaattcaa tgaacatacc gaagttagct actgagctga caataccttc
     3781 aaattcatca ccaatatgtt gaatcatata ttctgctttt ttcaattcat cagtatcacg
     3841 ttctgcctca atagcacgac gttcacgttt agaagtatgt tcagctaact caggcaattt
     3901 gtcttcccaa cgcttcactt ctttgttatc cattgatttc tcaattaaat acttacggat
     3961 taaacgatga actgttaaat caggataacg tctaattggt gatgtaaaat gcgtataata
     4021 ttcagctgat aagccaaaat gtcccaagtt cacatcatca taatgcgctt gttgcattga
     4081 acgcaacatc attgttgaaa tgaccatttg ttcaggtcga ccttctactt cttcttgaac
     4141 cttttgaagt gttgttggat gaatatcttc gccagtaccc ttaatcatga tgccaaagtt
     4201 tgtaataaaa tcaaagaatt gtcttaagcg atctgattta ggttgctcat gcactcggta
     4261 aataaaaggt acatctaact tactaaaatg ttcagcaact gtttcatttg caattaacat
     4321 aaatgattca attagacgtt caccctcgcc acgttgtctt aattgaacat ctgttggtat
     4381 accgtcttcg ttaactaata cttttgcttc actaatatca aaatcgattt caccacgtcg
     4441 ttttctcatt tgaatcaaac gattagataa atcttgtgct aaatctagca taggcgtaat
     4501 ttcattatat tgttcgcgaa tgttaggatc cttttcagta ataatctgat ttaccgcatc
     4561 atacgtcatt cgataatcag aatgtataac actatcaaaa atttcatgtt taacaacgcg
     4621 accactagca tcgatttcca tgcgacagct tagagttaaa cgatcaacat taggattcaa
     4681 tgaacaaata ccattactta atcgatgtgg aatcattgga attacacggt caacaagata
     4741 tacacttgtc gctctatcat atgcctcttt atccaatgca gaaccttctg ttacataata
     4801 gctgacatca gcaatactta cagttaattg cgtattaccg ttcgctaact ttttaacact
     4861 aattgcgtca tctaagtctt tagcatcagc accatcaatt gtgattgtca attcatcacg
     4921 taaatcatga cggcctttaa tttcagtatt ttcaatatga tcaggtactg cttcagcttc
     4981 ttgtaacact tcatcaggaa attcaatttc tatgccatgt tgatagataa tagataaaat
     5041 atctacgcca ggatcatttt tatgtcctaa aatagcagaa atatgtcctt ctggattatc
     5101 tgaaccatca gcatacttag taatttgtac aagtacctta tgaccatcga ctgcgcctaa
     5161 actttgacct ttaggaatga aaatatcttg cataatacgt ttatcatccg gaataacaaa
     5221 gccaaaatgt ctagcttcac tatacgtacc aacaacttga gttacagaat gcttctcaat
     5281 cgacttaact tccccttcga ttttaccttt atgttcacct tttgattgat ggatttctac
     5341 aataacagta tctccatcca aggcacgatt aattttcgtc gggggaataa atatatcttc
     5401 catatcctca tcttcaggtc ttaagaatgc aaagcctttt ttattttgac ttaacgttcc
     5461 ttttatcaat tttgattgac ctctataact atgctttttt tggtatctgt ctgtttttgt
     5521 acgttcgatt aatcctgatt gttctaactc cacaagcacc ttaattaaat ctctaaacga
     5581 gtcggcactg cttaaaccta atgcatcttg aaaatctgac actgacatag gttcatattc
     5641 aggttgatta ataatctctt ctatagattg ctttaaattc attatgcccc tcctttctaa
     5701 tgttaaaatc tctttttatt ctgaccagtc taatgactct aaaaattgat aaatatcttc
     5761 aaatacttgt tctttctctt tatcaatcgt aataacatgt ccagattcac tgtaccactt
     5821 gatatttttg tcatcagaat ctacatggtc atatatataa tttgcggatt gtggatcaat
     5881 catattgtcg ttttctgctt gaatcactaa aataggatcc aacacttcat caacttgctc
     5941 tttaatcgta tctaatgctt cacttagttc ttttaaagtt tcagttggtt taaaatgatc
     6001 catttcatta tcaatagtct cttgatcttt accttcatac tttttaaaat tgcgtgcata
     6061 ttctaaaaag ccttcataaa tggcaccttc agttttgcca cccataggag cacacatcgt
     6121 tacaataccc tttacatctc tatttaagct taattttaaa gcaaaatccc cacctaatga
     6181 tagaccagca acaacaattt catcataacc ttgttcaaca agataatcat aaccatctaa
     6241 cgcatcttta aaccaaacga aaggactaga tttcagtatt tcatctggtg gtgccgcgtg
     6301 gccttcatat tgcggtgcat atgatgtata acctttcttt tgtaaaaatc gacctaattg
     6361 acgaacatca gacgaattgc ctgtaaaacc atgtagtaat aacacggcac gtttaccttc
     6421 ctcaaaaaag aaaggttttg gtaattttat ctgcataaat ctaacgctcc ctttgcatac
     6481 ttaactaggc tatatatttt acatagctta ttgttaaact attacattct taattataaa
     6541 taaaaaagcc cgacatttac atcgccggac cttacatacc aagataactt atgcaaatca
     6601 taagtacaaa aaataatatt gataaaataa ttgttaatct att
//
LOCUS       DAKTGS010000146         6637 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.147, whole genome shotgun sequence.
ACCESSION   DAKTGS010000146 DAKTGS010000000
VERSION     DAKTGS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.147"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(135..1490)
                     /gene="brnQ"
                     /locus_tag="O0286_002452"
     CDS             complement(135..1490)
                     /gene="brnQ"
                     /locus_tag="O0286_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0170792.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            complement(1766..2323)
                     /locus_tag="O0286_002453"
     CDS             complement(1766..2323)
                     /locus_tag="O0286_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCY0170793.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(2390..4030)
                     /locus_tag="O0286_002454"
     CDS             complement(2390..4030)
                     /locus_tag="O0286_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCY0170794.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            4224..4325
                     /locus_tag="O0286_002455"
     CDS             4224..4325
                     /locus_tag="O0286_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170795.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(4304..6349)
                     /locus_tag="O0286_002456"
     CDS             complement(4304..6349)
                     /locus_tag="O0286_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0170796.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
BASE COUNT         2187 a         1145 c         1180 g         2125 t
ORIGIN      
        1 atgtgatttg taaaatgaaa gtactaatat tttttgtgga taatcattga tatgtatgta
       61 tacgattgca gtttaatgta gtatgaataa aaaatgccac aagcatatgt ttaacatatg
      121 actgtggcat agtattatgc ttgtggaatt ttacgatgct taattttata aataatgaag
      181 ccgataatga aaccaatcaa actgagaaca acccagccca taccaatgtc tgataatggt
      241 aaatattttt ggctgaaatt aatcaaagtt tgtgagaatg atgtgcttga aatgaactct
      301 ggactagctt ttaatccatc tactaatgca gcaatcattg taaagaaaat ggtacattga
      361 taaataagtt ttgaatgatg gaatttgcta ctaaataatg ttagtacaat caaggcaatt
      421 gctaatggat ataagaacat taacactggg actgagtaca taataatctt agttaaacca
      481 acattcgcga ataagaacga aataaagctt acaactgttg caatcgctag gtaattcatt
      541 ttagggaaaa ggtgttcgaa tgtttctgaa aatgccgtaa tcaaaccgat ggctgttttt
      601 aaacaagcaa ccataacgat aagtgacaac aggacgatac cgtagttacc taagtagtat
      661 tgagtaattt gcgctaaggc aataccacca ttttcactaa gtttgaaatg accaatactt
      721 aatgtaccca tgattgctag tagggtataa atgatcccca tcataatgat actgatagta
      781 ccagacttaa ttgtttcttt agcgatatca gttggatttt cgatacctaa ctttttaatc
      841 gttgcaacaa tgataatacc aaatgccaat gacgctagcg catctaaggt attgtatcca
      901 tctaaaaagc cgttaaataa ggcatgtgat tgatattgtt tactaatagg tgcatcagat
      961 atgccaccta atggatggat aaaagcaaat aataaaataa ttgctaataa tactaagaat
     1021 accggattta aaaatttacc gatatattct aaaattcttg atggctttct cgcaaaaaac
     1081 catgcaatca caaagaagac gaagctaaaa ataaataaat ataaagtgat ttgctttggt
     1141 gataaaaatg gcgaaaatgc aatttcaaat gatgtcgttg ccagtctagg taaggcgaaa
     1201 aatggtccga taactagata taaggcaatc gtgaaaatgt aagcatatgt tttattaaca
     1261 cgcgatgcaa tttcaaataa accagatgtc tttgaaatgc caatagcaat gatacctaga
     1321 aatggtaagc caattgctgt aattaaaaat cctaagttag cgataaaaac gttagaacca
     1381 gcagcttgac ccaagtgtat tgggaagata agattgccgg caccaaagaa taaaccaaat
     1441 aacatagaac ctataaacat gttttcttta aatgttagtt tcttcttcat atatgtattt
     1501 gttctccttt ttagaactta taaaaatcta cttaattaag ataatatcaa aaattaaaca
     1561 taaagtgata cataattttg caataaatac taaatttttt gaaaattccg gaataaatac
     1621 tgttttttat gtagtagggg cgtaagttaa gaacgattta acagatatag aatatgttaa
     1681 atatagaagg ttagacaacc cgacgtatta atactttgtg aaataaagat ccccctcaac
     1741 atgatcgaag gggggaagta tatgtttagt tcaatatatt tagaaagtcg tttgtagtta
     1801 ttgtttgtcc catcaatggg aatacattat ctattggaaa ttgatgtagc gtttcgtttt
     1861 gtgcactcat catatctgta acaaaaaact gattgtagtt taattgatag gcatctcgcg
     1921 ctgtcgtatc tacgccaata tgcgttgcga caccaccaag aacaatcgta tcaattcctc
     1981 gacgtcgcaa ttgtaagtcc aaatctgttc ctacaaatgc actaaaatgt cgtttgtcta
     2041 tgacaaaatc gtcatctctc ttgtctaata aatgatggaa acgactgtag tcgtcgcctt
     2101 cttttggtgg taatgagatc attgcatttg gttgcaatac atctttacca tcatagaaat
     2161 tcacgcgaac aaaagcgata aagccattgt tttttctaaa aacatctatt aatttattag
     2221 cgttttgaac gacattttca gctgtatatg gggcataatc cattttaaga ataccttctt
     2281 gcaggtcgat taacactaaa gcggttttat taaaattaat catcttatat cagagtccta
     2341 ttcgtttaat aaagtattcg cttaaactta taccctgtat acgatgaaat tatttatttt
     2401 gttgtgaaaa agctttagcg atatcgatga gtttcttcgg tgcgtcttcg acagccattt
     2461 tgacttcgac aaaatgcatc acatcgggat gaccattaat tgcattaaac gtgtcttgta
     2521 aatcttttga tgattcaacg tcatgaattt caacattttt accaccaaat acagctggta
     2581 aagctttata atcccacatg tgaatttcat tataaggttc atacatgccg tgaataagtc
     2641 gttctaccgt atagccgtca ttattaatca caaataatac cggtttaata tgctgtctaa
     2701 tcatagttga aatagcttga acagttagtt gcaatgagcc atcaccaatt aataataagt
     2761 tacgacgatc tttgtctgct aattgtgaac ctaatgttgc aggtaatgta tagccgatag
     2821 aaccccataa cggttgccct ataaaagtat tgtttttgta taatgctaaa tcataagcac
     2881 caaagaatga tgtaccttga tcagcaatga tgacatcatt tggttttaag aaattttgca
     2941 tcattttaaa ataagtttgt tgtgttaatg gttctgtgcc aacagtataa tcgggtgatg
     3001 ttggacgatg atacgcaggg aacgttgcgt tattcgtata tgaaatattg gataactgtt
     3061 ttaacaatga tggtagagat atttcatcat ttgtaacatc gtcaattttg atattgtgat
     3121 gatttaacat aacgacatca tcgatattga attggtatga aaaacctgct gttgctgaat
     3181 ctgttaattt ggctccaata tttaaaatta aatcgctgtt gtccacataa tctcgtattt
     3241 tatcttcggc aattttccca tcgtaaatac ccatataata tggattttcc tcattaaaag
     3301 caccttttcc taatgaaagt tgtgctactg gtatctgtgt ttgatttaca aaatcttcta
     3361 attcttgatg gaggtgaaaa ctgttaattt catgtccagt aatgatgata ggctgcttcg
     3421 cttgatgcag tttagttgct aataactcta tatatgttga tgcatccgta tatttagttg
     3481 ccgtcacttc aaatggtgtc ggtatctcaa tttcagagat tgcgacatcg attggtaaat
     3541 gtaaatgaac tgggcgtctt tcggcgattg ctgtattaat taaacgtggt atttcggttg
     3601 ttgcattttc aggtgtgata taaccttgtg caacggttat atgtgcaaac atttttcgat
     3661 agtcgtcaaa tgtaccttca ccaagtgagt gatgtacata tttaccgcct tgttcaacag
     3721 cacgtgtcgg cgcacctgta atcgcaatga caggtatgcg ttcagcatat gaacctgcga
     3781 taccgttgac ggcacttaat tcgccaacac caaatgtagt aactaatgca gcgagtccat
     3841 taagacgggc ataaccgtcc gctgcgtaac ttgcgtttaa ttcatttgta tttcctaccc
     3901 aatctacatt gggattgctg ataatatcgt ctagaaaagc gagattaaaa tcaccaggaa
     3961 caccaaaaat tttatcgacg cctgctcgat gaatagcgtc aattaagtaa gctccaatgc
     4021 gttgtttcat aaaaaaagcc accttttctc tatatttaca cttaaaatga tagcctcgaa
     4081 tatagtgtaa ggtcaaggaa gttgactttg ttgtgaaaac gctaacaata gtgaacaatt
     4141 aatatttttt aggaaatgag tatgaagggg ggctttaatt gatgatctta aaattcaaaa
     4201 tttaagggga gagcgaacta tcaatgatgc tgcaaaatac aatagcaaag aaaaaacagg
     4261 cgcgtcacct tatgaaggta tacgcacctg tttatagtaa gcattattta gcttcaaata
     4321 attgatcgcc aaatgaaatg ttgccatgtt caccttgttt aaaatcaagg tttgtaatgt
     4381 ttccttgtgt cacgataata ggcgtaatat cactctttgc atgattgcgg atgtagtcta
     4441 aatcaaagtt gattaataaa tcaccttgtt taacttcttg accttcctca acatgtaaag
     4501 taaagccttc tccgtttaat ttaacagtgt ctaaaccgat gtggattaat agttctaaac
     4561 cactatctga tacaagacca attgcatgtt ttgttgggaa aatcatttgt actttaccgt
     4621 tgaatggtgc acgaacttca ccttgtgaag gtttgatagc gataccgtca cccatcattt
     4681 tttcgctgaa cacttgatca ggcacttctg ataatggtgt tacttcacca gttaatggtg
     4741 catgcacgat atggctcaat tcgcttgttg cagatttatc ttctgcaaca acaacagttt
     4801 cgtctttatc gtcttccata gtagtaggat tttctactac ttgaccattc ataatctgtt
     4861 gcatttcatg tttgatttgg tcagatttag gaccaaaaat tgcttgcata ttattgccga
     4921 cttctaatac accagatgcg cctaaatctt tcaaaccagg aacatcaact ttagatttgt
     4981 cgttaacttc aacacgtaga cgtgtgatac aagcgtctaa atgtttaatg tttgctttgc
     5041 cacccatagc ttctaatact gcatatggta attcagttgc tgaagcagta gccgcttgtg
     5101 attgtttatc ttcacgacct ggtgttttgt attttaattt tacaattaag aatcggaata
     5161 cgaagtagta aataactgcg tatacaagac ctacaggaat gactaaccac cattgtgtct
     5221 tattaggtag tataccgagt aagaagtagt cgatgaaacc acctgagaat gtataaccta
     5281 gatgaagatc taataagtac aatgttaaga atgataaacc atcaagtact gcgtgaataa
     5341 agaataataa tggtgctaca aataagaatg agaattctaa tggttctgta ataccagtta
     5401 agaatgatgt taaagcagca gaacccatta aacctgctac tactttctta ttttcaggtt
     5461 tagctgtgtg ataaattgct aaagctgctg caggtaaacc gaacatcata acagggaatt
     5521 caccttgcat gaatttacca gctgtcaaat gtgcgccttc acgaatttgt tcgataaaga
     5581 tacgttggtc accgtgaata atttcaccag ctgcattttt ccatgaacca aactcgaacc
     5641 agaacggtgc gtggaaaatg tgatgtagac cgaatggaat taataaacgc ttgatgaaac
     5701 caaataagaa tacggcaaca ccagtatttg aatctaataa tcctgtactg aatgcattta
     5761 atcctgattg aatcgttggc caaattaatg ccattgggaa tgctaaaata aatgatgttg
     5821 tagccatcat aataggtacg aaacgcttac cagcgaagaa acctaaataa gatggtaagt
     5881 taatgttata gaacttgtta taacaccaag ctgccagggc cccgattata ataccgccga
     5941 acacacctgt ttgtaatgtt gggataccta aaatgctagc gtaaccactc gctggatcac
     6001 caatattctt aggtgtaact tgtaaaaagt cgcccattgt tttgttcatg attatgtaac
     6061 cgacgaatgc tgcgatagct gctacgccat caccgccagc taatccgatt gcgacaccta
     6121 atgcgaaaat cataggcaag ttatcaaaaa tgataccacc agcacctgtc attaatttag
     6181 cgacagtttg tacgccacca ttttgtataa acggcaagta gtgttgtaat gattcacctt
     6241 gcatagctgt accgatagct aataacagac cagctgctgg taaaatcgca acaggtaaca
     6301 ttagcgcttt accaatacgt tgcaattgac cgaaaagttt cttcctcact tgtccaacct
     6361 ccaaagttgt atttattatt taagattcaa taaaaaaaga cacgagcaaa tagactgtgt
     6421 tggtaaccat cacagcttaa tttaactcat gcctaatctt acttagtaac acgttggtgt
     6481 atgtaattaa tgtaaaagaa gcaattgact aagtagtata taaaactgat acgttagttt
     6541 atctagctta ccatcacatc ttattgaatc ttatttgttt gcgactccta ttttagcatg
     6601 gtgaagtatg cgttttcaat acaattatta aattcaa
//
LOCUS       DAKTGS010000147         6559 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.149, whole genome shotgun sequence.
ACCESSION   DAKTGS010000147 DAKTGS010000000
VERSION     DAKTGS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.149"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(12..143)
                     /locus_tag="O0286_002457"
     CDS             complement(12..143)
                     /locus_tag="O0286_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170797.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
     gene            complement(220..420)
                     /locus_tag="O0286_002458"
     CDS             complement(220..420)
                     /locus_tag="O0286_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170798.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            664..1149
                     /gene="tnpA"
                     /locus_tag="O0286_002459"
     CDS             664..1149
                     /gene="tnpA"
                     /locus_tag="O0286_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCY0170799.1"
                     /translation="MSSDTNSLAHTKWNCKYHIVFAPKYRRQVIYGKIKKDIGIILRQ
                     LCERKGVEIIEAEACKDHIHMLVSIPPKLGVSSFVGYLKGKSSLMIFDRHANLKYRYG
                     NRKFWCKGFYVDTVGRNKKVIENYIRNQLQEDIVADQISMEEYLDPFTGEKNKKRKKK
                     E"
     gene            complement(1285..2877)
                     /gene="pckA"
                     /locus_tag="O0286_002460"
     CDS             complement(1285..2877)
                     /gene="pckA"
                     /locus_tag="O0286_002460"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCY0170800.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      3032..3142
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3248..4441
                     /locus_tag="O0286_002461"
     CDS             3248..4441
                     /locus_tag="O0286_002461"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCY0170801.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            4566..5474
                     /locus_tag="O0286_002462"
     CDS             4566..5474
                     /locus_tag="O0286_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCY0170802.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            5686..6519
                     /locus_tag="O0286_002463"
     CDS             5686..6519
                     /locus_tag="O0286_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0170803.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         2269 a          992 c         1192 g         2106 t
ORIGIN      
        1 ttaccgcaat attaattgtt gtcatatact gacttttcac tattgtaaaa tgaatagtta
       61 ttatctttat tttcacaaaa tgaccgtagt ttaaacattt cacacatcat ttcaatgtgc
      121 gattttgaac ttggaagtaa catttcataa tgtatgaatt caaaaggagt atgtaaacaa
      181 tataatgttt aaacctcatc acttttaaat attctaattc taatgagaaa tacaaatttt
      241 tttattttct aaaatagcgt aatcatgcgt tttatttact attcttaaaa aatattcaaa
      301 aaaagtttta gtttctaatt gacaatgatt ctcactaatg tataatagta gttgaaaatg
      361 attatcaata ccatatagaa catccccccc acaacgtttc gttcttgttg gattggtcat
      421 tttcaaatat tcccctttta tatgcccgta aaagacaata tacgttataa caacgtttta
      481 taaaagcagt aaacccttac gacactttag gtttactgct tttgtttttt gtaaaaagaa
      541 aaccatacgc tatgcgtatg gttcagaaaa ggttctacca ttgtcacaaa aaatgcatct
      601 ctacgtgcta gaataaatat tggtcagcca accaaaataa tcaacacgag gagatgctat
      661 ttaatgtcat ctgacacaaa cagtttagca catacaaaat ggaattgtaa gtatcatatt
      721 gtctttgcac ctaaatacag aagacaagtg atatatggaa aaataaaaaa agatataggg
      781 attatattgc gtcaattatg tgaaagaaaa ggtgtagaga taattgaagc agaggcatgt
      841 aaagatcata tccatatgtt agttagtata ccacccaaac ttggggtatc atcatttgtt
      901 ggctatttaa aaggaaaaag tagtttaatg atatttgata gacatgctaa cttaaagtat
      961 agatatggaa atagaaagtt ttggtgtaaa ggattttatg tggatacagt aggtagaaat
     1021 aaaaaagtga ttgaaaatta tattcgtaat caattacaag aggatatcgt tgcagaccaa
     1081 atctcaatgg aagaatattt agatcctttc actggagaga aaaataaaaa aagaaagaaa
     1141 aaagagtaac cctttagggt tgctggaata gtagtgcagt tggctgactt gtcagtgccc
     1201 ttttagggct ggccagtaag gaaggcttat agccgcagaa caaaccaccc gttcacacgg
     1261 gtggttttga tttagtattc aaatttattt gttgaagcta cctttttcag caatatgttc
     1321 aactttttca ccaaattttt cgaagttctt ttcaaaacgt tgaattaaat cttctgcttg
     1381 tgctttatat ttctctttgt cgctccaagc attaattgga tttaaaattg ttttcggtac
     1441 atcttcaatt tctacaggaa tgcttaaacc aaacgtacta tcttttgtat attctgcatt
     1501 tttcaatttg ccagaaatcg cttggtttac catttgacgt gtgtaatgta agctgattct
     1561 acgtcctaca ccatattttc cgccagtcca tccagtatta acaagataaa catcaacatc
     1621 atgtaaatcg ataagttcac ctaatagatc agcgtaaaca gtagggtgta acgggaagaa
     1681 cggtgctccg aaacatgttg agaatgatgg ttcaggttct gtcacaccac gctctgtacc
     1741 agctaattta gaagtgaaac cactcaagaa atgatacatt gcttggtctt tatttaactt
     1801 tgaaatcggt ggaataacac caaatgcatc cgcagttaag aaaataattg tatttggatg
     1861 tgctgcttta gatggtacta caatattgtc aatgtgatta attggataag cggcacgcgt
     1921 gttttctgta taacgattgt cttcaaagtc cactgaacca tcttctgcaa ctacagtgtt
     1981 ctctaaaatt gcaccatatt tgattgcgtc aaaaatctgt ggttcttttt ctttggaaag
     2041 attaattgct tttgcatagc agccaccttc gatattaaag accccgtttt tattccagcc
     2101 gtgttcatca tcaccgatta gtttacggtg tgggtcagcc gataaggttg ttttaccagt
     2161 gccagataga ccaaagaata atgcaacatc gcctttttca ccaacgtttg ctgagcaatg
     2221 catgctcata atatcttgca tcggtaagag ataattcatt acagagaaga tacctttttt
     2281 catttcacca gcgtattcag taccgccgat taaaatgact ttgtgtttaa atgaaataat
     2341 gacaaaggtt tcagatttag taccatcaac ttctggatct gctttaaaat gtggtgcaga
     2401 aacgatagtg aagttaggtt taatctttgt agcttcttct tttgattcag gtctaataaa
     2461 catattttta gcaaataaat tatgccatgc taattcattg atgactgtaa gttttaacat
     2521 tgtatcttta tcgctaccag cgtagccttt aaatacgtat agttcatctt ttttatctaa
     2581 atagtctagt actttatggt ataacttcaa gaaagtttct tcatcgatag gttgattaat
     2641 ttctccccaa tcaatgttat ctctatatga aggttcagag acaaaaaatt tgtctttagg
     2701 cgaacgacca gtatattttc cagtacttgc attaacagca ccaagttctg ttaatacccc
     2761 ttcgttattg tctaagattt tattataaag ttgtgtcgtc gaaagttgaa aatgtgacgt
     2821 tggttttttc agtaatttgt caattttagt tgtttcagtg tatgtgtcta ctgacatcct
     2881 aaatccctcc aaagcgataa tttaaatgta agcctttaca tttaattagt ataacacatt
     2941 aacataatta atctacaata ttttaccgta aatttatttg tgaaaaattg acattaattc
     3001 attaattagg tactattatt tttaacggat tctcttatcc tgagtggtgg agggacatgg
     3061 acccaatgaa acccagcaac ctctttttta taaaagaaag gtgccaaacc gtttgcagac
     3121 aaataggtct gaacgataag agcgaatgga cgtattaggc cttctctcta tattaatagt
     3181 tagaaggtct tttttattta gctcacagag agagaatttt cgtaatataa atttaaagga
     3241 gcaaactatg ttaaataaca aacgattatt tacttcagag tctgttacag aaggacaccc
     3301 agataaaatc gctgaccaag tgtcagatgc aatattagat gctattttaa aagacgatcc
     3361 aaatgcacgt gtagcttgtg aaacaactgt tacaacaggt atggcattga tcgccggcga
     3421 aatttctaca acaacatatg ttgatattcc gaaagttgtt agagaaacaa ttaaagaaat
     3481 tggatacaca agagcaaaat atggttatga ttatgaaaca atggcaattt taactgcgat
     3541 tgatgaacaa tcacctgaca ttgcacaagg cgtggataaa gcattagagt atcgtgataa
     3601 agatagcgaa gaagaaattg aagcaacagg tgcaggtgac caaggtttaa tgtttggtta
     3661 cgcaacgaat gaaacagaga cgtatatgcc tctagcaatt tatttatcac atcaattggc
     3721 aaaacgttta tcagatgtgc gtaaagatgg tacgttaaat tatttacgac cagacggtaa
     3781 agttcaggtc actgttgaat atgatgaaaa tgataaccca gtacgtattg atacgattgt
     3841 cgtatcaaca caacatgctg aggatgtgac acttgaacaa attcaagaag acattaaagc
     3901 acatgtcatt tatccaactg ttccagaaaa cttgattaat gaacaaacta aattctacat
     3961 caatcctaca ggacgctttg taatcggtgg acctcaaggt gatgcaggat taacaggacg
     4021 taaaatcatt gttgatacgt acggtggcta tgcacgtcat ggtggcggat gcttcagtgg
     4081 taaggatcct acaaaagtag accgttcagc tgcatacgct gcacgttatg tggctaaaaa
     4141 tattgttgca gcaggtttag cagatcaatg tgaagtacaa ttggcatatg cgattggtgt
     4201 tgcagaacca gtgtcaattg caattgatac atttggaaca ggtaaagttt ctgaagggca
     4261 acttgtcgaa gcagttagaa aacactttga cctaagacct gcaggtatta ttaaaatgct
     4321 tgatttgaaa caaccgattt acaaacaaac tgctgcttat ggtcattttg gacgtacaga
     4381 tgtcttattc ccatgggaaa aattagacaa agtggaagaa ttaaaagacg cagtaaaata
     4441 ataagatgaa ttgaatatag ctaaataaaa gattttcggt atataaatga taatatgtat
     4501 cgaaaatctt tagcttttct attataatta aagttattca tatattaaaa aggagcgttg
     4561 ttgtgatgga tttatcttca ccgatagtca ttggactcat tattgcagta gtcattgcat
     4621 taatcttttt tgtccttttt ttagttgcct taggtagtaa gaaaaaagtt aagcgacaaa
     4681 cagaagaaaa gtatgaacaa caagaacaaa acattaaaaa gtctcatgaa gaagctttag
     4741 agaaagagcg tattcaaaat aaaaagacaa ttacaaaaca acaagaagat tataatcaca
     4801 tggttagtac aaaagatcgt gagattgacg cattaaaatt gttctcgaaa aatcatagtg
     4861 aatatgtaac ggacatgaga ttaattggta ttcgtgaaag attagttaaa gaaaaacgaa
     4921 ttcgaccaga agatatgcac attatggcaa atatcttttt accaaaggat ggctttaata
     4981 atattgaacg aattagtcat ttagtattaa caagaactgg tctttatatt attgattctc
     5041 aattgttaaa aggtcatgtt tataatggta ttagtggcgg tcaatttaaa gatttaccac
     5101 caatggaaca agtatttgat acattggatt tagataaatc tagaccgcaa actattgtga
     5161 tggatcaaaa tgatgacaaa cgttcattat catttgtaaa ttattcagat caaattgagg
     5221 cgattaaaca attggctgaa gacttacaaa aagaattagg tgccaaatat acaccaactt
     5281 caattttata ctttaatctt aaaaatgaag gcgatgtaac gatttcaaat tataatcaaa
     5341 atagtgctgt caaagtgctt gtcggtgctg aacaattaga cgagttcttt aacaaatttg
     5401 tattccatgg tcgtattcaa tacaacgtgg aagacttaca acaaatgatg gataaaattg
     5461 aatcattcaa ttaaatgtta ggctgaagtg atgactatat gttgttactt cggctttttt
     5521 agtttgtcat aaattaaaca gaattacttt tacaatttga ttaatatttg agagatatta
     5581 ctagaatttt agtggttgat tgctctaagt gttttttctc atgtattcaa ctcagatttg
     5641 ttatgataac agggtataat acatcaaaaa ggtgaggcga tatttatgga ggttaaaaca
     5701 ttttataatg gaaacacgat gccacaaatt ggtttaggga catttcgtgt ggaaaatgat
     5761 gaaaattgca tggaaagtgt taagtatgcc attgaacaag gatatcgtag tattgatacc
     5821 gcaaaagttt atgggaatga agaacaagta ggtgctggca ttcgtgccgg attggagtca
     5881 actggtattg ctagagaaga cttatttatt acttcaaaat tatattttga ggatttcggt
     5941 cgtgaaaatg tagcagctgc ttacgaagct agtttatcta gattaggttt aaaatactta
     6001 gatttgtatc tagtacattg gccaggtacg aacgaagccg taatggttga tacatggaaa
     6061 ggtatggaag atttatataa aaataataat gttaaaaata taggtgtcag caattttgaa
     6121 cctgaacatt tggaagcttt gttggcacaa gtgtcaatta aacctgtaat taatcaagtt
     6181 gaatatcatc catatttaac ccaacataaa ttgaaattat atttggcagc acaacgtatc
     6241 gtgatggaat cttggtcacc attgatgaat gcacaaattt taaatgatga gacaattaaa
     6301 gacattgctc aagaattagg aaagtcacct gcccaagttg ttttaagatg gaatgtgcag
     6361 catgctgtgg ttacaatccc taaatcggtg acaccaaaca gaatctctga aaatttccaa
     6421 atatttgatt tcgaattatc agatgaacaa atgacgcgaa ttgatggttt aaatcaagat
     6481 aagagaattg gacctgatcc aaaaaaattt gaaggctaga ttaaaatcgc tcaactgatg
     6541 aaaaggttag atgaattgt
//
LOCUS       DAKTGS010000148         6543 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.150, whole genome shotgun sequence.
ACCESSION   DAKTGS010000148 DAKTGS010000000
VERSION     DAKTGS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.150"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            372..1433
                     /locus_tag="O0286_002464"
     CDS             372..1433
                     /locus_tag="O0286_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0170804.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            1691..3148
                     /locus_tag="O0286_002465"
     CDS             1691..3148
                     /locus_tag="O0286_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCY0170805.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            3141..3863
                     /locus_tag="O0286_002466"
     CDS             3141..3863
                     /locus_tag="O0286_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0170806.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            4014..5141
                     /gene="queG"
                     /locus_tag="O0286_002467"
     CDS             4014..5141
                     /gene="queG"
                     /locus_tag="O0286_002467"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCY0170807.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            5146..5616
                     /gene="trmL"
                     /locus_tag="O0286_002468"
     CDS             5146..5616
                     /gene="trmL"
                     /locus_tag="O0286_002468"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCY0170808.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            5775..6374
                     /locus_tag="O0286_002469"
     CDS             5775..6374
                     /locus_tag="O0286_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCY0170809.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            6399..>6543
                     /locus_tag="O0286_002470"
     CDS             6399..>6543
                     /locus_tag="O0286_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170810.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEED"
BASE COUNT         2345 a          826 c         1225 g         2147 t
ORIGIN      
        1 tttaacttct ttttttggta ttacattaat gataatgatt atattatctg gggttatata
       61 tttgtttcaa cttttctaat aatatatttg taaaagtgct attttattgt gctttatacc
      121 aagtcgattc aatttatcta tcttaatagt aatatggcta ttttaatatt tacaatttat
      181 tacattatta gtcacaggat gtaattagta gtattttaaa attaacatga cattgattag
      241 tgagtagatt gtttaaattt gctttattca atttgtggat tgttatattt agattaaatt
      301 agccaactaa tcattgttaa agttaatttt ctataatatg atgagtttaa tgttcaaatt
      361 ggaggtcgtt tatgagtaac gcaaataatg ctgataataa acagtttttc agtaagattt
      421 taaattctat tggtgctggt gttgttattg cacttgttcc gaatgcttta ttaggtgaag
      481 tattaaaaat atttaaaagt ggtaatgaaa ttttagaatt aacgtatcag ttagtgatac
      541 taattcaatc atttatggca tttattattg gggttttagc tgctcatcaa tttaaattta
      601 atggtgcagg tgctgctata gtaggtacat cagctatgat tggttcaggt gctgttgtat
      661 atagcaataa tagttttatg ttaaaaggta taggtgacat tataaatacg agtttggttg
      721 ttattattgc atgtcttata tatatggtat tacaaaacaa acttggatct tttgaattaa
      781 ttatattacc agttttagta cctatcgtta gtgggggtat cggtctaatc acattacctt
      841 acattcgaaa aattactcaa gcaattggaa atgtaattca ttcttttaca gacttaaatc
      901 cgttgttaat gtctatactt attagtgtgg cattttcatt attaatggta acaccaatat
      961 cgttagtagc gattgcaact gcgattagtt taaacggact aggaagtgga gcagcaaacc
     1021 taggtatagt ggctgcctgt gttactttct tatttggctc attgcgtgtc aattctattg
     1081 gtgtaaatgc agttttactc ataggagctg caaaaatgat gatcccagtt tacttgaaaa
     1141 atttaattat ttcgatacca ttgactataa atggaataat tactggaatc attgcttacg
     1201 ttcttcaggt taaaggaaca ccattgtctg ctggatttgg atatacagga ttagttggcc
     1261 caataaatgc atttaaccga atgtcaggtg atccaacaat gaatattatt ttattagcac
     1321 ttggttattt tgtcatacca tttgtatcgg catttattgt tcatgaattg tgtaaaaaat
     1381 tcattcctat atattctaat gatatttata aatttgaagt tccaaaacaa taatttatag
     1441 cttgatgaca agaataagtt ttaatatatg ataaatatag gcgttactgt caataagata
     1501 ttgatagtag cgcttttttg attgatttta aaatgtgaga tttgttcacg tataatagcg
     1561 gattttttat gtacagtctt ttaaaaagaa gcttttcttt atattgataa aaatacattt
     1621 ataaaacact attgcatcaa aatgtattaa tatgtataat tatgaatatt aattagggag
     1681 ggctgtataa atgaagtgtt taattaggtt tatattagta ttagggttgt taattagcag
     1741 tgctatggtg tatatcaatc ccacagcaca tgctgaacaa gatcaaacat gggagaagat
     1801 taaagaacgc ggtgaactta gagtgggtct ttctgcagat tatgcaccaa tggaatttga
     1861 gcatacagtt aatggtaaga ctgagtatgc aggtgtagat attgatttag ctaaaaaaat
     1921 tgcgaaagat aataatttaa aattaaaaat cgtcaatatg tcatttgata gtttgttagg
     1981 agctcttaaa actggaaaaa ttgatattat tatttccgga atgacttcaa cgcctgaacg
     2041 taagaagcaa gttgattttt cagattcata tatgatgact aaaaatatca tgcttgtaaa
     2101 gaaagataaa gttaatgaat ataaagatat caaagacttt aataataaaa aagtaggggc
     2161 acaaaaggga actgaacaag aaaaaatcgc tcaaaccgaa attgaaaatg catctattac
     2221 ttcattaagc cgtttaccag acgttatatt agcattgaaa agtggaaagg ttgaaggtgc
     2281 tgtagttgaa aaacctgtag cagaagcata tttaaaacaa aatcctaaat taggaatttc
     2341 caatgtgaaa tttaatgaag aagaaaaaga tacagtgata gcagtgccaa aagattcacc
     2401 aaaattattg tcacaaatta ataaaacgat taaggaggtt aaagataaag gattaatcga
     2461 taaatatatg actaatgctg caaatgcgat gaatgatgac agtggtttta tttctaagta
     2521 tggtagtttt ttcttgaaag gaattaagat tacaatatta atttcactta tcggtgttgc
     2581 attaggttct attttaggtg cattcgttgc gttaatgaaa ttaagtaaaa taaaaattat
     2641 ttcatggatt gcttctatct atatcgaaat attaagagga acaccaatgt tagttcaagt
     2701 atttatcgta ttctttggta taactgctgc attaggacta gacatttcag ctcttgtttg
     2761 tggaacaatt gcattagtta ttaattcctc agcttacatt gctgaaatta ttcgtgcagg
     2821 tataaatgct gttgataaag gccaaatgga agccgcacgt agtctaggtt tgaattatag
     2881 acaaacgatg aaaagtgtaa ttatgccaca agcaattaaa aatattttac cagctttagg
     2941 taatgaattt gtcactttaa ttaaagaatc atctattgtg tcaacaattg gagttggcga
     3001 aattatgttt aatgcacaag tggttcaagg tatttcattt gacccattca caccattaat
     3061 agtggcagca gcattatact ttgtcttaac atttgtactt acacgcatta tgaacatgat
     3121 tgaagggaga ttgaatgcca gtgattaaaa taaacaatct taataaagtt tttggagata
     3181 atgaagtttt aaaagatatc aatcttgaaa tcaatcaagg ggaagtagta gcaataatag
     3241 gtccatctgg tagtggtaaa agtacattgt taagatgtat gaatttatta gaagtaccta
     3301 ctaaaggtca agtgattttt gaaggcaatg acttaacgga aaaagggaca caagtagata
     3361 aactacgtca aaaaatgggt atggtatttc aaaacttcaa cctatttcca cataaaaaag
     3421 ttgtcgataa tattatttta gctcctaaat tattaaagaa agataataac gatgaattac
     3481 ataaggaagc attgtcgtta ttagataaag tgggattaaa agaaaaagca gatgtatatc
     3541 cgaatcaatt atcaggtggt caaaaacaaa gggtagcaat tgcaagagct ttagcaatgc
     3601 atccagatgt tattttattc gatgaaccaa cttcagcatt agatcctgag gtagttggtg
     3661 atgtattaaa agtaatgaaa gacctagcca aagaaggtat gaccatggtg gttgtgacac
     3721 atgaaatggg atttgccaaa gatgtaagtg acaaagtcat atttatggca gatggcgttg
     3781 tcgtagagtc aggcacacca gtcgaaatat ttgaacaacc gcaacatgaa agaacacaaa
     3841 atttcttagc aagagtatta taacaaccta acgaagcttg aatatatgat acgcaccaca
     3901 aagttatatc atatattcaa gctttttatt attaaaatta tgtgaaatga aacctcaata
     3961 tgaataatgg ttgtgttaaa ataacatagt attattttat ggggggcata cgattggata
     4021 caaagcaatt aaagcaagat atcattgact atgcatatac aattggtatc gacagtattg
     4081 gatttactac tgccgatccc tttgatgaat tgaagcaaaa gctagaagca tatcatgcaa
     4141 atggttatgc ctcaggattt gaagaatctg atattgcttt acgaacggag cctaaattat
     4201 ccttaccaac agcaaggtca atcatagcaa ttgcagttgg ttatcctaac aaactgaaag
     4261 gtgcacctaa gagtgttaga ggtgatcgca gaggcttatt tgctagagca tcgtggggtc
     4321 aagattatca tacaattatg cgtaaacgat tagacatgtt agctgcattt attgaatcta
     4381 aagttccaga tgttgaaatc aaatctatgg tagatacggg tgtattatca gatagggcag
     4441 tagcagaacg tgcaggttta ggatttgttg gtcgtaatgg ctttgtcatc aatcctaaac
     4501 taggaacatg gacatacctc ggtgaaatgt tagtcagtat accttttgaa cctgatgatc
     4561 cattattaga tagctgtggt gattgtacaa tttgtgttga tcgttgtcca acaagtgcat
     4621 tagttggaaa tggccaatta aatagtcaga aatgcattag ttttttaacg caaacaaaag
     4681 gctatatgcc tgaccaatat cgttataaaa ttgggaatag attatatggt tgcgatacgt
     4741 gtcaacaagt ttgtccgaaa aatagaggta ttaataccga acaagatgac atcattttgg
     4801 aaccagaaat tttaaagcca agattagtac ctttactacg tatgtctaat aaagaattca
     4861 aacaaacata tggtcacctt gcaggtgctt ggcgtggtaa aaagccgata caacgaaatg
     4921 ctattttagc attggcgcat tttaatgaag tggatgcaat tccagaattg aaaaaagttg
     4981 caacaacaga tgaaagaccg ttgattcgag caactgctta ttgggccatt ggtcaaattc
     5041 ttggggaaga agcaagagat tttatcaatg ctaattatga tcaagaggat gcagaagttc
     5101 agaatgaaat gataaaagga ttagacacaa ggagagaata gtaatatgac aaatcatatc
     5161 gttttatatc aaccagaaat cccagctaat actggtaaca ttgcacgtac gtgtgcaggt
     5221 acaaacacac atttacattt aattaaacct cttggattta gaactgatga taaaatgtta
     5281 aaaagagccg gtttagatta ttgggaattt gtgaatatta cgtatcatga tagtattgaa
     5341 gcattttttg aagtcacaaa tggcgagtat tacttattaa caaaatttgg taaaaagact
     5401 tatagtgatt ttgatttttc aaatcatgac aaagattttt acttcatttt tggaaaagaa
     5461 acgacaggat taccagactg ggtgaaagag aaatatcaag acacagcgtt aagaattcct
     5521 atgagtgaac atattcgttc attaaattta tcaaatactg ctgcgttgtt aatttatgaa
     5581 gcgttacgtc aacaagattt tccgggattg aattaaaatt tactcatttt cattataaaa
     5641 atgatgctta agtgctatat ttagagtata agcatgttaa agttaaatgg tttgatatat
     5701 tgaaagttag ggtataataa ttattaatat cgccgttttt atgtaaaatg caaactaaga
     5761 ggagtgtaga aatcatggca atgaacttta aagtctttga caatagtcaa cttgtagcag
     5821 aatatgctgc tgatattatt agaaagcaat ttaacaataa tcctactaca attgcaggtt
     5881 ttcatttaga tacagatcaa gcgccagttc tagatgaatt aaagaaaaat gttgaaaaac
     5941 atgctgttga ttttagccaa ataaatattt tagattatga cgataaaaaa tcatatttcg
     6001 aagcgttagg tgtaccagca ggtcaagttt atccaattgc ttatgaaaaa gatgcaatcg
     6061 aattaatcgc tgataagatt aaaactaaag aaaataaagg gaaattaaca ttacaagttg
     6121 tttctatcga tgagcaaggt aagttaaatg ttagtattcg tcaaggacta atggaagcaa
     6181 gagaaatttt cttagtagtg acaggtgcta ataaacgaga tgtagttgaa aaattatatc
     6241 aagaaaatgg taaaacaagc ttcgaaccag ccgatttaaa agcacataga atggtaaatg
     6301 ttattcttga taaagaagcg gctgcaggtt tacctgaaga tgttaaagct tactttacgt
     6361 cacgctttgc ttaattaaaa atctgtgagg tattgattat gtctaaagac aaagatccaa
     6421 aattaaatta tcatgaagaa gaaaacagta tggtaacgga ttttgaagat ttaaaagaat
     6481 taggtaaaga aatggaacaa atctctgatc aaaatgatca agaaaagaat tctgaagaag
     6541 aca
//
LOCUS       DAKTGS010000149         6527 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.151, whole genome shotgun sequence.
ACCESSION   DAKTGS010000149 DAKTGS010000000
VERSION     DAKTGS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.151"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            150..836
                     /gene="mtnN"
                     /locus_tag="O0286_002471"
     CDS             150..836
                     /gene="mtnN"
                     /locus_tag="O0286_002471"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCY0170811.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            856..1383
                     /locus_tag="O0286_002472"
     CDS             856..1383
                     /locus_tag="O0286_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCY0170812.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1384..2484
                     /gene="yqeH"
                     /locus_tag="O0286_002473"
     CDS             1384..2484
                     /gene="yqeH"
                     /locus_tag="O0286_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCY0170813.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            2498..3304
                     /gene="aroE"
                     /locus_tag="O0286_002474"
     CDS             2498..3304
                     /gene="aroE"
                     /locus_tag="O0286_002474"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCY0170814.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            3308..3598
                     /gene="yhbY"
                     /locus_tag="O0286_002475"
     CDS             3308..3598
                     /gene="yhbY"
                     /locus_tag="O0286_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCY0170815.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            3601..4170
                     /gene="nadD"
                     /locus_tag="O0286_002476"
     CDS             3601..4170
                     /gene="nadD"
                     /locus_tag="O0286_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCY0170816.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            4160..4744
                     /gene="yqeK"
                     /locus_tag="O0286_002477"
     CDS             4160..4744
                     /gene="yqeK"
                     /locus_tag="O0286_002477"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCY0170817.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            4745..5098
                     /gene="rsfS"
                     /locus_tag="O0286_002478"
     CDS             4745..5098
                     /gene="rsfS"
                     /locus_tag="O0286_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCY0170818.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            5101..5817
                     /locus_tag="O0286_002479"
     CDS             5101..5817
                     /locus_tag="O0286_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0170819.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            5857..>6527
                     /locus_tag="O0286_002480"
     CDS             5857..>6527
                     /locus_tag="O0286_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCY0170820.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKS"
BASE COUNT         2413 a          810 c         1280 g         2024 t
ORIGIN      
        1 tttatgatac caagttattg aaagagcttt ttataaaaat aaagcaaaag ttttatacag
       61 taatgaaaat catgatactt gtcgttgtag tgattaaaat gtgataaaat aacaaatgct
      121 aggatttttt aaaagaggtg aatcaaaaga tgattggtat aattggtgcc atggaagaag
      181 aagtaacaat attaaaaaat aaattaacac aattaagcga aatttcagtt gcacatgtta
      241 aattttatac tggcatttta aaagatagag aagtagtgat tacccaaagt ggcattggaa
      301 aagttaatgc tgcaatttct acgacattat taattaataa gtttaaaccg gacgtcatta
      361 ttaatacagg ttctgctgga gctttagatg aaagtttaaa tgtaggtgac gttcttataa
      421 gtgatgatgt aaaatatcat gatgcagacg caacagcatt tggttatgaa tatggacaaa
      481 taccacagat gccggtagca tttcaatcaa gtaaaccttt aatagaaaaa gtatctcaag
      541 ttgtacaaca acaacaatta acagctaaag taggcttaat tgtaagtggt gatagcttta
      601 tcggtagtgt tgaacaacgc caaaaaatta aaaaagcatt tccaaatgcg atggcggttg
      661 aaatggaagc aactgcaatt gcacaaacat gttatcaatt taatgtacca tttgttgtag
      721 ttcgtgcagt ttcagactta gcaaatggag aagcggaaat gagcttcgaa gcatttttag
      781 aaaaagcagc tgtatcatca agtcaaactg ttgaagcatt agtgtctcaa ttataaaaag
      841 gtaaaggtga gttaaatggg tttagttcgc aagtttttta tgccgaattc atatgttcaa
      901 tcaatatttc aaattgattt agacaagtta gtggacaaag gcgttaaagg tattattaca
      961 gatttagata atacgctagt aggttgggat gttaaagaac ctacagaacg tgttaaagca
     1021 tggtttaagg aagctaatga aaaaggaatc actattacaa tcgtgtctaa taataatgag
     1081 tctcgtgttg ctagttttag tcagcattta gacatcgatt ttatttttaa agcgagaaag
     1141 ccaatgggga aagcgtttga taaagcaata actaagatga atatcagacc agatcaaact
     1201 gttgttatag gtgaccaaat gcttactgat gtatttggtg gtaatcgtcg aggtctatat
     1261 acaattatgg ttgttccagt taaacgaact gatggcttta ttactaagtt taatagatta
     1321 attgaaaggc gattattacg tcatttcagt aaaaaaggtt atatcacatg ggaggaaaat
     1381 tgattgtctg acattttaaa atgtatcggt tgtggtgcgc cacttcaatc tgaagataaa
     1441 aataaacctg gttttgtacc agagcataat atgtttcgtg atgacgtgat ttgcagacgt
     1501 tgtttccgct tgaaaaatta taacgaagtt caagatgtag gattagaaag tgaagacttt
     1561 ttaaaattat tatcaggact tgcggataaa aagggtattg tcgtcaatgt cgtggatgta
     1621 tttgactttg aaggatcatt tattaatgca gttaaacgta ttgtcggaaa caaaaaaatc
     1681 attttagcag caaataaatt ggatttattg ccgaaacaaa ttaataaacg tcgagttaaa
     1741 gaatggttaa aacgaacagc aagaaaatat ggtttggaag ctgacgatgt cgtattaatt
     1801 tcagctgaaa aaggctgggg catagacgac ttattatcat caattgcgaa tattcgagaa
     1861 aatgaagatg tgtatattgt agggacaacg aatgttggga aatctacatt gattaataaa
     1921 ctgattgaag ctagtgttgg tgaaaaagat gtagtaacaa cttcaagatt ccctggaaca
     1981 actttagata tgatagatat tcctttagat gaaacatcat ttatgtatga tacaccaggt
     2041 attattcaag atcaccaaat gacgcattta gttagtgaaa aagaattgaa aattattatg
     2101 cctaagaaag aaataaaaca acgcgtatat caattaaatg aggcgcagac attattcttc
     2161 ggcggtctag cgcgcataga ttatgtatca ggtggtaaac gtccgttagt ttgtttcttt
     2221 tctaatgact tgaatataca tcgtactaaa acggagaagg ctaatgattt atggcgtaat
     2281 caacttggcg atttattaac gccacctgga aatccacaaa attttgatct taatgaggta
     2341 aaggctgtta gacttgaaac aggcaaagag aaacgcgatg ttatgatctc tggtctaggc
     2401 tttataacta taggaccagg ggctaaagta atcgttcgtg ttcctaaaaa tgttgatgtt
     2461 gtattaagaa attctatttt ataaggtgat taaaaaaatg aaatttgcag ttataggaaa
     2521 tcctatttca cattccttgt cgcccgttat gcatagagca aattttaatt ctttaggatt
     2581 agatgatact tatgaagctt taaatattcc aattgaagat tttcatttaa ttaaagaaat
     2641 tatttcgaaa aaagaattag aaggctttaa tatcacaatt cctcataaag aacgtatcat
     2701 accgtattta gattatgttg atgaacaagc gattaatgca ggtgcagtta acactgtttt
     2761 gataaaagat ggcaagtgga tagggtataa tacagatggt attggttatg ttaaaggatt
     2821 gcacagcgtt tatccagatt tagaaaatgc atacatttta attttgggcg caggtggtgc
     2881 aagtaaaggt attgcttatg aattagcaaa atttgtaaag cccaaattaa ctgttgcgaa
     2941 tagaacgatg gctcgttttg aatcttggaa tttaaatata aaccaaattt cattagcaga
     3001 tgctgaaaag tatttagctg aattcgatat cgttattaat acaacaccag cgggtatggc
     3061 tggaaataac gaaagtatta ttaatttaaa gcatctttct cccaatactt taatgagtga
     3121 tattgtttat ataccgtata aaacacctat tttagaggaa gcagagcgca agggaaacca
     3181 tatttataat ggcttagata tgtttgttta ccagggtgcg gaaagcttta aaatttggac
     3241 taataaagat gctgatatta attctatgaa aacagcagtt ttacaacaat taaaaggaga
     3301 ataacatatg cttactggca aacaaaaaag atacttaaga agtttagcac acaatattga
     3361 tccgattttt caaattggaa aaggcggtat caacgaaaat atgattaaac aaatagatga
     3421 tacgttagaa aacagagaat tgattaaagt acatgtacta caaaataact ttgatgataa
     3481 aaaagaatta gctgaaacat taagcgaagc tactcatagt gaattagtgc aagtgattgg
     3541 atctatgata gtgatttata gagaatctaa agataataaa gaaattgaat tgccataata
     3601 atgaaaaaga tagtacttta cggcggtcag tttaacccta tccatactgc acatatgata
     3661 gtagctagcg aagtatttca tgaattacag ccagatgaat tttatttttt acctagtttt
     3721 atgtctccat tgaaaaagca ccatgatttt atagacgttc agcacagatt aacaatgata
     3781 cagatgatta tcgacgagct tggttttgga gatatttgtg acgatgaaat taaacgtggt
     3841 ggtcaaagtt atacctatga cacgatcaag gcattcaagg agcaacacaa agacagtgag
     3901 ttgtactttg ttattgggac ggatcagtat aaccaactag agaaatggta tcaaattgaa
     3961 tacttaaaag aaatggttac ttttgtagtt gtaaatcgag acaaaaatag tcaaaatgtt
     4021 gaaaatgcta tgattgcaat tcagatacct agggtagata taagttcgac aatgattcga
     4081 caaagagtta gtgaagggaa atctatccaa gttcttgttc ctaaatccgt tgaaaactat
     4141 attaaggggg aaggattata tgaacattga aaaagcaaaa cggcttgcaa aagagaaact
     4201 gccagagaaa cgttataatc attctttaag agttgcagaa acagcgatta aattggcaga
     4261 aatttatgat ggagacacta gtaaagtaga attagcaggt gtattacatg atttctgtaa
     4321 atatgatgat ttaggtaaaa tgtatcaaat tgttcgacaa tacgaattag gtaatgatct
     4381 actgagttat ggtagtgaaa tattgcatgg ccctgtgtgt gcagcaatca tggaacatga
     4441 atatggtatc aatgatgagg aagtattaat ggctatcaaa taccatacta ctggacgtca
     4501 acaaatgaca aaaactgaaa aactgatttt tattgcagat tacatcgaac ctggaagaac
     4561 aatcccagga gttgatgata ttcgagatat ggcatacaat caaggtagtt tagataagac
     4621 aatttatgaa atttctaaac gcacagtact atttttaata cagaaagata ttacggtata
     4681 taataagacg attgactgtt taaattatta taactatagt gacgaaagaa taaaggatga
     4741 ttaaatgaat tcacaagaat tattagcaat tgctgtggat gcaattgaca ataaaaaagg
     4801 cgaagatacg atttctttag aaatgaaagg tatcagcgat atgacagatt attttgttgt
     4861 aacgcacgga aataatgaac gacaagttca agcgattgct agagcggtga aagaagtagc
     4921 caatgaacaa aatatagaag taaaacgtat ggaaggatac aatgaagcgc gttggatatt
     4981 aattgactta gctgatgttg tggtacatgt tttccataaa gacgaaagaa attattataa
     5041 tattgaaaag ttatatcaag atgcaccatt agaatcatat agtcaggttg cgtattaatt
     5101 atgtcgcaat atgcagaaat gagcctagtg tacgatcaat tgactcaaga tcaaccatat
     5161 gaaaaatggt ttgaaattgt aaaaaatcac tgcaaagatg aatcaaatat tttagatatt
     5221 ggatgcggta ctggtagttt aacagttcaa ttagaagctt taggtaatgt tactggtatg
     5281 gatttaagtg ttgatatgtt gactgttgca gctcaaaaat cagcaaatgt aaattggctt
     5341 gaaggagata tgacatcttt tgatttgcaa caacaatttg atatcatcac tattttttgt
     5401 gactctctaa attatttgca agatgaaact gcagtgattg aaacattcat caatgtttat
     5461 catcatctga ctgattcagg tgtatttatt tttgatgtac atactgttta taaaatgatg
     5521 actttgttta ataatcaaag ttatattgat gataaagggg acattttttt agcatgggat
     5581 gctgtgcaag gagatttacc tttaagtgtt tatcatgata tgacattttt cattcgacat
     5641 gaagatgaaa catattcaag atttgatgag tctcattttc agagaacatt tgatgaaaag
     5701 acatatttgt cttggttagc tcaagttggt tttaaacatg ttgaaacatt tactgatttt
     5761 aatatagatg aacataatga agatgcagaa agattgtttt tcattgcgaa aaaataaaag
     5821 taaaaccact cgctcagacg tgtataaagt tgaggagtgg ttttattgta tcaattttta
     5881 ttacgctata aagatttttt aactcaatgg aagttatata ttctaagtgc tgttgtttta
     5941 attatggtat taattggttt tatattctgg agacaagatg attatacttc aagaaatttt
     6001 gaaaataaag atactgctct gaaacaaagc actagtgaaa atagtagttt gtccaaagta
     6061 gaagatgtcc aggtcaaaga tggagataat tccaaaaata agggtcctgt atatgtcgat
     6121 gtaaaaggtg ctgttaaaca tcctaatgtt tataaaatga catctaagga tagagtagtt
     6181 gatttacttg ataaagcaca attattggag gatgcagatg taagtcaaat taatttgtct
     6241 gaaaaattaa cagatcaaaa aatgattttc atacctcata aaggacaaaa gaatgttgaa
     6301 ccacaaattg aagtaaacag tgtgcacgaa aaaaatggga acacaaataa tactaaagta
     6361 aatttaaata cggcatctgt atcagaattg atgtctgttc ctggagttgg gcaagctaaa
     6421 gctaatgcaa ttgttgaata tcgcaaccaa caaggtgcat ttcaagaaat tgacgatttg
     6481 aaaaaagtaa aaggttttgg aagtaaaact tttgataaac tgaaatc
//
LOCUS       DAKTGS010000150         6274 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.152, whole genome shotgun sequence.
ACCESSION   DAKTGS010000150 DAKTGS010000000
VERSION     DAKTGS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.152"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            144..860
                     /locus_tag="O0286_002481"
     CDS             144..860
                     /locus_tag="O0286_002481"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCY0170821.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            853..1878
                     /locus_tag="O0286_002482"
     CDS             853..1878
                     /locus_tag="O0286_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0170822.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            1900..3588
                     /locus_tag="O0286_002483"
     CDS             1900..3588
                     /locus_tag="O0286_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0170823.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            3621..5345
                     /gene="tarS"
                     /locus_tag="O0286_002484"
     CDS             3621..5345
                     /gene="tarS"
                     /locus_tag="O0286_002484"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0170824.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            5485..6159
                     /gene="scdA"
                     /locus_tag="O0286_002485"
     CDS             5485..6159
                     /gene="scdA"
                     /locus_tag="O0286_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCY0170825.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
BASE COUNT         2301 a          855 c         1157 g         1961 t
ORIGIN      
        1 tgctaaaata taagcaaaat atgtataatt ttaagtaata cacagacatt tagagttttg
       61 attttaaaat gagttttcaa ttggaaaatg caacgaaatt taaataatta aatatagata
      121 tagttgaatg gaggaagtat tttatgaaat acgctggtat tctagctgga ggtataggct
      181 caagaatggg taacgtacct ttacctaaac aatttttaga tttagacaac aaaccgattt
      241 taatccatac attagaaaaa tttattttaa ttaatgattt tgaaaaaatt attatcgcga
      301 cgccacaaca atggatgacg catacgaaag atacacttag aaaattcaaa atttctgatg
      361 aaagaattga agtcattcaa ggtggtagcg atcgtaacga tacaattatg aatatcgtta
      421 aacatattga atcaacaaat ggtattaacg atgacgatgt cattgtgaca catgatgcag
      481 ttagaccatt tttaacgcat cgtattatta aagaaaatat tcaagctgct ttagagtacg
      541 gtgcagtaga tacagtgatt gatgctatag atacgattgt tacatctaaa gataatcaaa
      601 cgattgatgc aattccagtg cgtaatgaaa tgtaccaagg tcaaacacct caatcgttta
      661 atattaattt attaaaagaa agctatgcac agttgagtga tgagcaaaag agtattttat
      721 ctgatgcttg taagattatt gtagaaacaa acaaaccggt tcgacttgta aaaggtgagt
      781 tatataacat taaagtaaca acaccttacg atttaaaagt agcgaatgct attattcgag
      841 gtggtattgc cgatgattaa tcaagtatat caattagttg cacctagaca atttgaagtt
      901 acgtataaca acgtagatat ttacagtgac tatgtcattg tacgtccttt atatatgtca
      961 atttgtgctg ccgatcaaag atattatact ggtagccgtg atgagaatgt cttatctcag
     1021 aaattgccaa tgtctttaat tcatgaaggt gttggtgagg tcgtatttga cagtaaaggt
     1081 gtgtttaata aaggtacaaa agtagttatg gtaccgaata cgccgacaga aaaagacgat
     1141 gtcattgctg aaaactattt aaaatcgagc tacttcagat caagtggaca tgatgggttt
     1201 atgcaagatt ttgtgttgct aaatcatgat agagctgtac cactacctga tgatattgat
     1261 ttaagtatta tttcatatac agagcttgta acagtaagtt tgcatgctat tcgtcgtttt
     1321 gaaaagaaat ctatttcaaa taaaaataca tttggtattt ggggtgatgg taacttaggt
     1381 tacattacag ccattttatt acgtaaatta tatccagagt ctaaaatata tgtctttggt
     1441 aaaacagatt ataaattgag tcacttctca tttgttgatg atgtcttctt tattaataaa
     1501 atacctgaag gcttaacatt tgatcatgca tttgagtgtg tgggtggtcg cggtagtcaa
     1561 tcagccataa atcaaatgat cgattacatt tcaccagaag gaagcattgc actgttaggt
     1621 gtaagtgagt tcccagtaga agttaataca cgtctagtat tggaaaaagg actaacgttg
     1681 attggtagta gtcgaagtgg ttcaaaagat ttccaagatg ttgtagactt atacattcaa
     1741 tacccagata ttgtagataa attagcgttg ttaaaaggtc aagaatttga aattgcaaca
     1801 attaatgatc ttacagaagc ttttgaagca gacctgtcta catcttgggg taaaacagta
     1861 ttaaaatgga ttatgtaaat agaaaaggat gaattaacgt tggttaaaag taagatatat
     1921 atagataaaa tctattggga acgtgttcag ttattcgttg aaggacatag tgaaaaccta
     1981 gatttagaag atagtaattt tgtattaaga aatttaactg agacacgtac aatgaaggcg
     2041 aatgatgtca aaatagatgg gaatcaattc gtttgtcgtt tcaatgtagc tatcttagat
     2101 aatggttatt acttacctga agataagtac ttattagtga atgagcaaga acttgattat
     2161 attgcacagt taaacccaga tgtgattaat gatgcatatc aaaatctaaa gccagaacaa
     2221 gaagaagaat acaacgaatt agaaacacaa aatggtaaaa tcaatttctt attgcagact
     2281 tacctaaaag aatttagaaa aggcggcatt tcgaagaaaa cggtttatac tgttacacct
     2341 gaaatttcta gcgatgttaa tgaatttgtc cttgatgttg ttgtaacgac tccggaagtt
     2401 aaaagtattt atatcgttcg taaatataaa gaattacgta agtatttccg caaacaatca
     2461 tttaatacaa gacaatttat ttttaaagcg atatttaata cgacgaaatt tttccacttg
     2521 aaaaaaggga atacggtgtt gttcacatca gactctagac caacgatgtc tggaaacttt
     2581 gaatacatct ataacgaaat gttacgtcaa aatttagata aaaagtatga tattcacact
     2641 gtttttaaag cgaatattac agatagacgt ggcatcatcg acaagtttag attgccatat
     2701 ttacttggga aggcagacta catctttgtt gatgactttc acccattgat ttatacagtg
     2761 cgttttagac gttctcaaga agttattcaa gtatggcatg ccgttggtgc ctttaaaaca
     2821 gttggcttta gtcgtactgg taaaaagggt ggaccattta ttgattcatt aaatcatcgt
     2881 agctatacaa aagcttatgt atcatctgaa accgatattc cattctacgc tgaagcattt
     2941 ggtattaaag agaaaaatgt agtgcctaca ggtgttccac gtactgatgt actatttgat
     3001 gaagcttatg cgacacagat caaacaagag atggaagatg aattaccaat tattaaaggt
     3061 aagaaagtca ttcttttcgc accaacattt agaggtagtg gtcatggtac agcacattac
     3121 ccatttttca aaattgattt tgaacgttta gcaagatatt gcgaaaaaaa taacgcggtt
     3181 gtattattta aaatgcatcc atttgtgaaa aatagactta atattgcaga caaacataaa
     3241 caatattttg ttgacgtttc tgactttaga gaagttaatg atatactgtt cataacagat
     3301 ttattaatta gtgactattc atctttaata tatgaatatg cagtatttaa aaagccaatg
     3361 attttctatg catttgattt agaagattat attacgacgc gtgattttta tgaaccatat
     3421 gaatcatttg ttccaggtaa aattgtgcaa tcatttgacg cattaatgga cgccttggac
     3481 aatgaagatt atgaaggaga aaaagtcatt ccattcttag ataaacattt taaatatcaa
     3541 gatggccgat caagtgagcg tttagtcaga aatttatttg gtagctaagt ttatatagta
     3601 gtcaaagtgg gagaggtata atgatgaaat tttcagtaat agttccaaca tacaattcag
     3661 aaaagtatat aacagaatta cttaatagcc ttgcgaaaca agattttccg aaaactgaat
     3721 ttgaagtggt tgtagttgat gactgttcaa cagatcaaac gttacaaata gttgaaaagt
     3781 atcgcaataa attgaacttg aaagtaagtc aactcgaaac aaattctggt ggtccaggta
     3841 aacctagaaa tgtggcgtta aaacaagcag aaggtgaatt tgtattattt gtggactccg
     3901 atgactatat aaacaaagag actttaaagg atgcagcagc atttattgat gaacatcact
     3961 cagatgtctt attgattaaa atgaaaggtg ttaatggtcg tggtgtacca caatctatgt
     4021 ttaaagaaac agcacctgaa gttactttgt taaattcaag aattatctat actttaagcc
     4081 cgactaaaat ctatagaaca gcattactaa aagataatga catttatttt ccagaagaat
     4141 taaagagtgc agaagatcaa ttatttacaa tgaaagcata tttaaatgca aatcgaatca
     4201 gtgtgttaag tgataaagcg tattattatg ctacaaagcg tgaaggtgaa catatgagta
     4261 gtgcgtatgt ttcacctgaa gacttttatg aagtcatgag attgattgct gtagaaatat
     4321 taaatgcaga tttagaagaa gcccataaaa atcaaatctt agcagaattt ttaaatcgtc
     4381 attttagttt ttctcgtacg aatggcttct cacttaaagt taaactagaa gatcaaccac
     4441 aatggattaa tgctctagga gactttatac aagcagttcc agaacgtgta gatgcattgg
     4501 tgatgagtaa attacgacca ttgttgcact acgcgagagc gaaagatata gacaactata
     4561 gaactgtgga ggaaagttac cgtcaaggtc aatactaccg ttttgatatt gtagatggta
     4621 aattaaacat tcaattcaat gaaggcgaac catactttaa aggcattgat atcgctaagc
     4681 caaaagtgaa aatgacagca tttaaatttg ataatcataa aattgttaca gagctaacgt
     4741 taaatgaatt tatgattggc gaaggacatt atgatgtcag acttaaatta cattcacgaa
     4801 acaagaagca cacaatgtat gtacctttaa gtgtcaatgc gaataaacaa tatcgtttta
     4861 acattatgtt agaagatatt aaagcgtatt tacctaaaga aaaaatttgg gatgttttct
     4921 tagaagtcca aataggtacg gaagtatttg aagtgcgtgt tggtaatcaa cgtaataaat
     4981 atgcatatac tgcagaaaca agtgcattaa ttcatttgaa taatgatttt tatagattaa
     5041 caccgtattt cacaaaagac tttaataaca tttcgttata ctttacagct attacattaa
     5101 cggattcaat ctcattgaag ttaaaaggta aaaacaaaat cattttaact ggtctggatc
     5161 gtggttatgt atttgaagaa ggtatggcta gtgtcgtact aaaagacgac atggtgatgg
     5221 gaatgttaag ccaaacatca gaaaacgaag tgcaaatctt acttagcaaa gatattaaaa
     5281 agcgagactt caaaaatatt gttaagttaa acactgcaca tatcacttat ccactaaata
     5341 aataataaat gccctcaaat cattgtgagc caacatgatt tgagggcttt attttgctgt
     5401 ttatgacatg attatgacat ttccctgatt ttcattttca tatacattaa attgtataca
     5461 ctggaaatga ggaggttatc tataatgata aataaaaatg acatagtagc agatgtagta
     5521 actgattatc cgaaagcagc ggatattttt agaagtgtgg gaatagattt ttgttgtggc
     5581 ggacaagtaa gtatagaagc agcagcctta gaaaagaaaa atgtagattt gaacgaatta
     5641 ttacagcgtc tcaacgacgt tgaacaaacg aatacaccag gttcgttaaa tcctaaattt
     5701 ttaaatgttt catcacttat tcaatatatt caatcagcat atcatgaacc tctaagagaa
     5761 gaatttaaaa atttaacacc ttatgtgacg aagttatcga aagtacatgg acctaatcat
     5821 ccatatttag ttgagttaaa agaaacatac gatacattta aaaatggcat gttagagcat
     5881 atgcaaaaag aagacgatgt cgattttcca aaactcatta aatatgagca aggtgaggta
     5941 gtagacgata ttaatactgt gatagatgat ttagtttcag accacattgc aacgggagaa
     6001 ttgttagtaa aaatgagcga attaacatct agttatgaac ctccgataga agcgtgtggt
     6061 acttggcgac ttgtttatca gagattaaaa gcacttgaag tgttaacaca tgaacacgta
     6121 catttagaga atcacgtatt atttaaaaaa gtatcataaa taacgcgatt agaaactgtt
     6181 ggcaaaaata agtccagcag tttttcgcta tgtataaaag tcataatagt gacataaaca
     6241 gcattatttg aaaagaaaaa tggtcaactt agca
//
LOCUS       DAKTGS010000151         6097 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.154, whole genome shotgun sequence.
ACCESSION   DAKTGS010000151 DAKTGS010000000
VERSION     DAKTGS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.154"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..785
                     /gene="ftsW"
                     /locus_tag="O0286_002486"
     CDS             <1..785
                     /gene="ftsW"
                     /locus_tag="O0286_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCY0170826.1"
                     /translation="KKMPRVLSKPKLILSPIVLALGCTFLVFLQKDVGQTLLILIILV
                     AIIFYSGIGVNKVLRFGIPAVLGFLVVFVIALMAGWLPSYLTARFSTLTDPFQFESGT
                     GYHISNSLLAIGNGGVFGKGLGNSAMKLGYLPEPHTDFIFAIICEELGLIGGLLVITL
                     EFFIVYRAFQFANKTSSYFYKLVCVGIATYFGSQTFVNIGGISATIPLTGVPLPFISF
                     GGSSMISLSIAMGLLLIVGKQIKVDQQRKKQQQKVDIRRQFN"
     gene            1339..4791
                     /locus_tag="O0286_002487"
     CDS             1339..4791
                     /locus_tag="O0286_002487"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCY0170827.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(4931..5842)
                     /locus_tag="O0286_002488"
     CDS             complement(4931..5842)
                     /locus_tag="O0286_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCY0170828.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
BASE COUNT         2163 a          915 c         1138 g         1881 t
ORIGIN      
        1 gtaaaaaaat gcctagagta ttaagtaaac caaaattaat tttaagtcct attgtattag
       61 cattaggttg tacgttttta gttttcctac aaaaagacgt agggcaaaca ttactaatat
      121 taattatttt agttgcgatc attttttatt caggaattgg ggtaaacaaa gtcctaagat
      181 ttggtatacc agcagtgcta ggattcttag tagtatttgt cattgcatta atggctggtt
      241 ggttaccaag ttatttaact gccagattta gtacgctaac agatccattc caattcgaat
      301 caggaactgg ataccatatt tccaattcat tgcttgcgat aggtaacggt ggcgtatttg
      361 gaaaaggatt aggaaatagt gcaatgaaat tgggctattt accagaacca catacagatt
      421 ttatttttgc aattatttgc gaagaattag gtttaatcgg aggattgcta gttattactt
      481 tagagttctt tattgtatat cgtgccttcc agtttgcaaa taaaacatca tcatattttt
      541 ataaacttgt gtgtgttggg attgccacat actttggaag tcaaacgttt gtaaacattg
      601 gcggtatttc ggcaacaatt ccattaactg gtgtgccatt gccatttatc agctttggtg
      661 gatcatcaat gattagttta agtattgcta tgggtttact tctgattgta ggtaaacaaa
      721 tcaaagtaga ccagcaacga aagaaacaac aacaaaaagt tgatataaga agacaattta
      781 attaaaaaaa tactagccaa tatttagtac gagatgactt ctgtcattaa gcaatgctaa
      841 acattggcta tttatatact ctttgtcaaa ttggactgat gagttcaata acggaagtta
      901 ggcaaccaaa caacgcttaa cttccttttt gttttgatat tacgagatta ctcatgatta
      961 tcaaatttta taaacaaaac aacgtataat tataactaaa ttttatgact aaaaattact
     1021 aaatgtggtg gcgaaattag aatcatgatg ataagataaa gttatcgcta ttgataataa
     1081 atgttgatgt tttgcaattt tatttttaat tttttagagt tgtagaaatc gaaaatgttt
     1141 cacaacaaaa acgtgaacta cctgttgttg catagtcatt tttgaaatta ttatttctga
     1201 agcggaggtt ttaattgtct caagaatgtt aaattttgaa aatttgaaac ataactatag
     1261 caaaacagag cgctttaaga taaaatttta ttatctaatt agttagaaaa ttctaatagt
     1321 ttgaggagtg tatcgctatt gaaacaaata aaaaagttac ttgttgctaa ccgtggagaa
     1381 attgcaattc gtatattcag agcggcggca gaattagaca tcagcacagt tgcaatttat
     1441 tcgaatgaag acaaaagttc attacataga tataaagcag atgaatccta tttagttgga
     1501 agtgatttag gtcctgctga aagttattta aatattgagc gtatcattga tgtagcaaaa
     1561 caagcgaatg tggatgcgat tcatcctggc tatggatttt taagtgaaaa tgaacaattt
     1621 gcgcgtcgtt gtgcagaaga aggaattaaa tttattggtc ctcatttaga acatttagat
     1681 atgtttggag ataaagttaa agctcgtaca acggctatca aggcagattt accagttatt
     1741 cctggtacag acggtccaat taaatcatat gaattagcaa aagaatttgc agaagaagct
     1801 ggtttcccgc taatgattaa agccacaagt ggtggcggcg gtaaaggtat gagaatcgtt
     1861 cgtgaagaaa gtgaattaga agatgctttc catagagcaa aatcagaagc tgaaaaatca
     1921 tttggtaata gtgaagttta catagaaaga tacattgata atccaaagca tattgaagta
     1981 caagtcatag gtgacgaaca tggaaatatc gtacacttat ttgaacgtga ttgttcagta
     2041 caacgtcgtc atcaaaaagt tgtagaagtt gcaccatcag ttggattatc atcaacatta
     2101 cgtcaacgta tttgtgatgc tgcaattcaa ttgatggaaa atattaaata tgtcaatgca
     2161 ggtactgttg aatttctagt atctggtgac gaattcttct ttatagaagt taaccctcgt
     2221 gtacaagtag agcatacaat tacagagatg gtaacaggaa ttgatattgt taagacacaa
     2281 attttagttg cagcaggtgc cgatttattt ggtgaagaga ttaatatgcc gcaacaaaaa
     2341 gatattacaa cattaggcta tgccatccaa tgtcgtatta caacagaaga tccgttaaat
     2401 gatttcatgc cggatactgg aacaatcatt gcttatcgtt caagcggggg ctttggtgta
     2461 cgtcttgatg ctggagatgg tttccaaggt gctgagatat caccttatta tgattcatta
     2521 ctcgtaaaat tatctacaca cgcgatatca tttaaacaag cagaagaaaa aatggtacgc
     2581 tcattacgag aaatgcgtat tcgtggtgtt aaaactaata ttccattctt aattaatgta
     2641 atgaagaata aaaagttcac aagtggtgat tacacaacta aatttattga agaaacacca
     2701 gaacttttca acattcagcc gtctctagat agaggtacta aaacattaga atatataggt
     2761 aatgtaacaa ttaatggttt cccaaatgtt gagaaacgtc cgaaaccaga ctatgaatta
     2821 gcatcaattc caactgtatc ttcaagtaaa atcgcttcat ttagtggtac gaaacaattg
     2881 cttgatgaag taggtccaaa aggtgtagct gaatgggtta aaaagcagga tgatgtctta
     2941 ctaacagata caacctttag agatgcacac caatcattat tagctacacg agttagaact
     3001 aaggatatga ttaatatcgc atccaaaaca gcggacgtat ttaaagatgg tttctcacta
     3061 gaaatgtggg gcggtgctac atttgatgtg gcatataatt tcttgaagga aaacccatgg
     3121 gaacgacttg aacgtctacg taaagctatt ccaaatgtat tattccaaat gttgttacgt
     3181 gcttcaaacg cagttggtta taaaaactat cctgataatg ttattcataa attcgtacaa
     3241 gaaagtgcta aagcaggcat agatgtcttt agaattttcg attcattaaa ctgggtagat
     3301 caaatgaaag ttgccaatga agcagtacaa gaagcgggca aaatctcaga aggtactatt
     3361 tgttatacag gtgacatttt aaatcctgag cgatcaaaca tttatacttt agagtattat
     3421 gtcaaactag ctaaagagtt agaacgtgaa ggtttccata ttttagcgat taaagatatg
     3481 gcaggcttat taaaacctaa agccgcttac gaattgattg gtgagttaaa atcagctgta
     3541 gatttaccaa ttcatcttca cactcatgat acaagtggta atggtttatt aacatacaaa
     3601 caagcaatag atgctggtgt cgatatcatt gatactgctg ttgcttcaat gagtggttta
     3661 acaagtcagc caagcgccaa ttcgttatat tatgcattaa atggcttccc acgccacctt
     3721 agaactgata ttgaaggtat ggagtcactt agtcattatt ggtcaactgt acgtacttat
     3781 tattcagact ttgaaagtga tatcaaatca ccgaatactg aaatttatca acatgaaatg
     3841 cctggtggac agtattcgaa tttaagtcaa caagctaaaa gtttaggttt aggcgaaaga
     3901 tttgatgaag tcaaagatat gtatcgcaga gtgaatttct tatttggtga tatcgtaaaa
     3961 gtaacaccat cgtctaaagt agttggtgat atggcacttt atatggtaca aaatgatctt
     4021 gatgaacaat ccgtgattac agatggctat aaattagatt tcccagaatc agtagtgtcg
     4081 ttcttcaaag gtgaaatagg acaacctgta aatggtttta ataaagattt acaagcggtt
     4141 attttaaaag gccaagaagc actaacagct cgtccaggtg aatatctaga gccagttgat
     4201 tttgaaaaag tccgtgagtt gcttgaagaa gagcaacaag gtcctgttac ggagcaagat
     4261 attattagtt atgtactata tccaaaagta tatgaacaat atattcaaac tagaaatcaa
     4321 tacggaaact tatcgttact tgatacgcct acattcttct ttggaatgcg taatggtgaa
     4381 acagtagaaa tcgaaatcga taaaggtaaa cgattaatta ttaaactaga aacgattagt
     4441 gaaccagatg aaaatggtaa tagaacgatt tactatgcga tgaatggtca agcgagacgt
     4501 atttacatta aagatgaaaa tgtgcataca aatgcgaacg ttaagccaaa agcagataag
     4561 agtaatccaa gtcatatcgg tgctcaaatg ccaggttcag taactgaagt caaggttagt
     4621 gtaggtgaaa ctgtgaaagc taatcagccg ttgctaatta ctgaagctat gaaaatggaa
     4681 acaacaattc aagcaccatt tgacggtgtg attaaacaag taactgtaaa taatggtgac
     4741 acaatagcga caggcgattt attaatcgaa attgaaaaag caactgacta aaatgattaa
     4801 aataaaacga gattacacaa caattgacgt gtaatctcgt ttttacatgg cttaggttta
     4861 attttaaata ttgatataaa aaaccgtaat tttaatgggc gtgccattaa aattacggtt
     4921 tgcttttttg ttattgcttg tcacttctta ctgatcgtaa cattagcatg ataaagtatg
     4981 ttgtcatacc aaataaataa gtgataaata atgcatggaa aagtgcgatt atcaggttaa
     5041 catttgtcat aatagataat gcacctgtga taacttgtaa tataacaagt ataaatgcag
     5101 ctgtataacc ataatgaaca gtacggttat ttggataatt tttaacagcg tgaatataag
     5161 taatcataat aatcgtaaac actataaaag ccatgatacg atgcgtgagt tgaacccaat
     5221 cttgttctga atgtggtaca agatcgtgga atggcaatgg ccaaccacca tatgctaaac
     5281 ttgcatccgc atgtctcact agtgcaccag tataaacacc acaataaatg atgattgcca
     5341 ttaaccatgt taaacgtctt aatggctttt tgatatataa ttcgtcagct tcatattttt
     5401 gatctataga gaaaataatc aatgttatta aaaatacaga tgagaaactg attaatgata
     5461 taccaaagtg caatgctaaa acgtaatcgt tttgttgcca aataacagca gcagctccga
     5521 ttaatgcttg caataataag aatccaacac taatgattga taaaggttta atttctttaa
     5581 tatagcctat atgtttccat gcagtgataa ctaaccataa gaccattaat aaagacaaag
     5641 ctgaaacggc tctatgactt aactcaataa tcgtatcaat aggaaagaat tctggaatca
     5701 acgcaccatg acatagtggc caagaagaac cacaaccatc agctgatccg gttttggtaa
     5761 ctaaggctcc accaagttgt acaaatgtca tcattaacgt tgctacgaca cctaaccatt
     5821 ttaaattctt tttgccaaac aatttataca ccccatcata aaaaaacgca tcactgagaa
     5881 ttagaattaa tagccggatt tgagatgata ctataaatag acctgattgt aggatatgaa
     5941 aagatgggat ccaacgcagg aaatatgatt taattttaca agtattgtat gtggacgaca
     6001 taaaaagaac ttaatcgtaa tgattttttt attgggataa ttatgtaatc cacgtttact
     6061 caaatttgaa gcttaaatat taatctcttt tagatgc
//
LOCUS       DAKTGS010000152         5877 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.155, whole genome shotgun sequence.
ACCESSION   DAKTGS010000152 DAKTGS010000000
VERSION     DAKTGS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.155"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            34..741
                     /gene="splA"
                     /locus_tag="O0286_002489"
     CDS             34..741
                     /gene="splA"
                     /locus_tag="O0286_002489"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCY0170829.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            866..1588
                     /gene="splB"
                     /locus_tag="O0286_002490"
     CDS             866..1588
                     /gene="splB"
                     /locus_tag="O0286_002490"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCY0170830.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1646..2365
                     /gene="splC"
                     /locus_tag="O0286_002491"
     CDS             1646..2365
                     /gene="splC"
                     /locus_tag="O0286_002491"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCY0170831.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2486..3205
                     /gene="splD"
                     /locus_tag="O0286_002492"
     CDS             2486..3205
                     /gene="splD"
                     /locus_tag="O0286_002492"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCY0170832.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(3154..3261)
                     /locus_tag="O0286_002493"
     CDS             complement(3154..3261)
                     /locus_tag="O0286_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170833.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            3363..4079
                     /gene="splE"
                     /locus_tag="O0286_002494"
     CDS             3363..4079
                     /gene="splE"
                     /locus_tag="O0286_002494"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCY0170834.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4230..4949
                     /gene="splF"
                     /locus_tag="O0286_002495"
     CDS             4230..4949
                     /gene="splF"
                     /locus_tag="O0286_002495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCY0170835.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            5014..5148
                     /locus_tag="O0286_002496"
     CDS             5014..5148
                     /locus_tag="O0286_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170836.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            5312..>5877
                     /locus_tag="O0286_002497"
     CDS             5312..>5877
                     /locus_tag="O0286_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0170837.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         2287 a          863 c         1044 g         1683 t
ORIGIN      
        1 aactataaaa ataaatttgg aaggaggtaa aatatgaata aaaatgtaat ggttaaaggt
       61 ttaactgctt taactatttt aacatctctt ggttttgctg aaaatatttc taatcagcct
      121 cattcaattg ccaaagcaga aaagaatgtc aaagaaatta ccgatgcaac taaggaacca
      181 tacaattcag tggtagcatt tgtgggtggt actggtgtag ttgttggtaa aaatacaatc
      241 gtaactaaca aacatatcgc taaaagtaat gatattttta aaaatagagt atcagcacat
      301 cattcgagta aaggtaaagg cggaggaaac tacgacgtta aagacattgt agaatatccc
      361 ggaaaagaag accttgcgat agttcatgtt catgaaacaa gtacagaagg tttgaatttt
      421 aataagaacg ttagttatac aaaatttgca gacggagcaa aagtgaaaga tagaatttct
      481 gttattggtt atccaaaggg tgcacaaaca aaatataaaa tgtttgaatc gacaggaacg
      541 attaaccata tcagtggaac gtttatggaa tttgatgcgt atgcacaacc aggtaattca
      601 ggatctcctg tattgaattc taaacatgaa ctgattggta ttttatatgc aggtagtgga
      661 aaagatgaat ctgaaaagaa tttcggtgtt tatttcacac cacaattaaa agaatttatt
      721 caaaataata ttgaaaaata agtatcatca attcattcgt gaagttgatt ttttaaagag
      781 tgattaagaa aacggttaca taattaacta aatatactga attatgtatc tagatatcaa
      841 aataattaaa agagaggaac ttaaaatgaa caaaaacgta gtcatcaaga gtttagcagc
      901 attaacaatt ttaacatctg taacaggtat tggaacaaca ttggttgagg aagtacaaca
      961 aactgccaaa gcagaaaata atgtcacaaa agttaaagat actaatattt ttccatatac
     1021 tggtgtagtt gcttttaaaa gtgcaactgg atttgtagtt ggaaagaata ctattttaac
     1081 aaataaacat gtgtcgaaaa attacaaagt gggcgatcgt attactgcac atccaaatag
     1141 tgataaaggt aatggtggta tttattcgat taaaaagatt attaattatc caggtaaaga
     1201 agatgtatca gtcattcaag ttgaagagcg tgcaatagaa cgtggaccaa aaggctttaa
     1261 ttttaatgat aatgtaacgc cattcaaata tgcggcaggg gctaaagctg gtgagcgaat
     1321 taaagtgatt ggttatccac acccatacaa aaataaatat gttttatatg agtcaactgg
     1381 ccctgtgatg tcagtagaag gtagcagtat tgtatattca gcgcatactg aaagcggaaa
     1441 ctctggatca cctgtattaa acagcaacaa cgaattagtt ggtattcatt ttgcttctga
     1501 tgtaaaaaat gatgataaca gaaatgcata tggcgtctac tttacaccag aaattaaaaa
     1561 attcattgca gaaaacatag ataaataaac aaattgactt taaacgagcg ttgcaacata
     1621 tctcgaattg taaaggagct tgaaaatgaa taaaaatata gtcattaaaa gcatggcagc
     1681 attagccatt ctaacctcag taactggaat aaatgctgca gtcgttgaag agacacaaca
     1741 aatagcaaat gcagagaaga atgttacgca agttaaagat acaaatattt ttccatataa
     1801 tggcgtcgtt tcatttaaag atgcgacagg ttttgtaatt ggaaaaaata caattatcac
     1861 caataaacat gtatcaaaag attataaagt tggcgataga attactgccc atccaaacgg
     1921 tgacaaagga aatggtggta tatataaaat taaaagcatt tctgattatc cgggtgatga
     1981 agacatctct gtcatgaata ttgaagaaca agcagtcgaa cgtggaccaa aaggctttaa
     2041 ttttaatgaa aatgtccaag cattcaattt tgcgaaagat gctaaagttg atgacaaaat
     2101 taaagttatt ggttacccat tacctgctca aaatagtttt aaacagtttg aatctacagg
     2161 aactataaaa agaatcaaag acaatatttt aaattttgat gcatacattg aacccgggaa
     2221 ttcaggatca ccagttctaa attctaacaa tgaggtcata ggtgtggtgt atggcggtat
     2281 tggaaaaatt ggttctgaat ataatggtgc cgtatacttt acgcctcaaa tcaaagattt
     2341 tattcaaaag cacattgaac aataaacaaa tttaaatata caccatgagc atgtgttcaa
     2401 taattttaat gaaaaacatc ggtcgaatat aacataaaaa aacgtctata tcaaaagcat
     2461 catgaataaa cagaggagca caaaaatgaa taaaaatata atcatcaaaa gtattgcggc
     2521 attgacgatt ttaacatcaa taactggtgt cggcacaaca gtggttgatg gtattcaaca
     2581 aacagccaaa gcagaaaata gtgtgaaatt aattaccaac acgaatgttg caccatacag
     2641 tggtgttaca tggatgggcg ctggaacagg atttgtagtt gggaatcata caatcattac
     2701 caataaacat gttacttatc acatgaaagt cggtgatgaa atcaaagcac atcctaatgg
     2761 tttttataat aacggtggtg gactttataa agttactaag attgtagatt atcctggtaa
     2821 agaagatatt gcggtcgtac aagttgaaga aaaatcaacg caaccaaaag gtagaaaatt
     2881 caaagatttc actagcaaat ttaatatagc atcagaagct aaagaaaatg aacctatatc
     2941 agtcattggt tatccaaatc ctaatggaaa taaactacaa atgtatgaat caactggtaa
     3001 agtactatca gtgaatggaa atatagtgac atctgatgcg gttgtccaac ctggcagctc
     3061 tggttcacct atattaaata gtaagcgaga agcaattggt gttatgtatg ctagtgataa
     3121 accaacaggt gaaagtacaa ggtcatttgc tgtttatttc tctcctgaaa ttaagaaatt
     3181 tattgcagat aatttagata aataaatcat ccatccatac attgataaat gatttttaga
     3241 aattaacaac aaaatcaaca attttaaaca tctctgtgat tctatttatt cgaaatgatt
     3301 taaaaaataa aacttcaaaa acctaacctt atatttatac gaatacttag aggagcacaa
     3361 aaatgaataa aaatataatc atcaaaagta ttgcagcatt gacgatttta acatcagtga
     3421 ctggcgtcgg cacaacagtg gttgagggta ttcaacaaac ggctaaagct gaacataatg
     3481 tgaaactaat caaaaatact aatgtagcac catacaatgg tgtcgtttcg ataggatctg
     3541 gaacaggttt cattgtcggt aaaaatacaa ttgttaccaa caagcatgtc gttgcaggta
     3601 tggaaattgg tgcacatatt atagcgcatc ccaatggtga atataataat ggcggatttt
     3661 ataaagttaa aaaaattgtc cgttattcag gtcaagaaga tattgccatt ctacatgtgg
     3721 aagataaagc tgttcatcca aaaaacagga attttaaaga ttacacaggc attttaaaaa
     3781 tagcatcaga agctaaagaa aatgaacgca tttcaattgt tggctatcca gaaccatata
     3841 taaataaatt tcaaatgtat gagtcaacag gaaaagtgct gtcagttaaa ggcaacatga
     3901 ttattactga tgctttcgta gaaccaggca actcaggttc agctgtattt aacagtaaat
     3961 acgaagttgt aggtgttcac tttggtggaa acggccctgg aaataaaagt acaaaaggat
     4021 atggtgttta tttctctcct gaaattaaga aattcattgc agataacaca gataaataaa
     4081 tccttacata gataaatgat tttaaaaatt aacaacaaac tcaacaattc aaatcatctc
     4141 tgtgattcca tttattcgaa atgattaaaa aaaataaaac ttcaaaaagc taacattata
     4201 attatacaaa tacttagagg agcagaaaaa tgaataaaaa tataatcatc aaaagtattg
     4261 cagcattgac gattttaaca tcaataactg gtgtcggcac aacaatggtt gaaggtattc
     4321 aacaaacagc caaagccgaa aatactgtta aacaaattac aaatacaaat gttgcaccat
     4381 acagtggtgt tacatggatg ggcgctggaa caggatttgt agttggaaat catacaatca
     4441 ttaccaataa acatgttacc tatcacatga aagtcggtga tgaaatcaaa gcacatccta
     4501 atggttttta taataacggt ggtggacttt ataaagttac taagattgta gattatcctg
     4561 gtaaagaaga tattgcggtt gtacaagttg aagaaaaatc aacacaacca aaaggtagaa
     4621 aattcaaaga tttcactagt aaatttaata tagcatcaga agctaaagaa aatgaaccta
     4681 tatcagtcat tggttatcca aatcctaatg gaaataaact acaaatgtat gaatcaactg
     4741 gtaaagtatt atcagtgaat gggaatatag tgtcatcgga tgcaattatt cagcctggta
     4801 gctctggttc acctatatta aatagtaaac acgaagctat tggtgtaatc tatgccggta
     4861 ataagccatc aggtgaaagc acaagaggat ttgctgttta tttctctcct gaaattaaga
     4921 aattcattgc agataattta gataaataat taaaacttag acattcaccc aatcctgaca
     4981 aaatatacta taactaacat ttattaatat atattgcatt atttaatatg catcaaagcc
     5041 aatcaacgat tgattttcac caactcaatt gttgattggt tttatttatg tatgaatgaa
     5101 caactttttg acatcattaa gaatataaat gattttgaaa gcatttgaaa gctacaacat
     5161 ttctataaaa tttttcaata acaattgcgc cactaaaact caaaatttcc accaccaaca
     5221 tccaaattat caacatcgca acataaccaa atgttataat aaatctatta cacaaagaga
     5281 taaattactt atgcaaaggc ggaggaatca catgtctatt actgaaaaac aacgtcagca
     5341 acaagctgaa ttacataaaa aattatggtc gattgcgaat gatttaagag ggaacatgga
     5401 tgcgagtgaa ttccgtaatt acattttagg cttgattttc tatcgcttct tatctgaaaa
     5461 agccgaacaa gaatatgcag atgccttgtc aggtgaagac atcacgtatc aagaagcatg
     5521 ggcagatgaa gaatatcgtg aagacttaaa agcagaatta attgatcaag tcggttactt
     5581 cattgagcca caagatttat tcagtgcgat gattcgtgaa attgaaacgc aagatttcga
     5641 tatagaacat ctagcgacgg cgattcgcaa agttgaaaca tctacattag gtgaagaaag
     5701 tgaaaatgac tttatcgggc tgttcagcga tatggatttg agttcaacgc gactaggtaa
     5761 caatgtcaaa gaacgtactg ctttaatctc taaagtcatg gttaatcttg acgacttacc
     5821 attcgttcac agtgacatgg aaattgatat gttaggtgat gcatatgaat tcctaat
//
LOCUS       DAKTGS010000153         5877 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.156, whole genome shotgun sequence.
ACCESSION   DAKTGS010000153 DAKTGS010000000
VERSION     DAKTGS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.156"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            171..1730
                     /gene="rny"
                     /locus_tag="O0286_002498"
     CDS             171..1730
                     /gene="rny"
                     /locus_tag="O0286_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCY0170838.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2027..2242)
                     /locus_tag="O0286_002499"
     CDS             complement(2027..2242)
                     /locus_tag="O0286_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170839.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            2416..3213
                     /locus_tag="O0286_002500"
     CDS             2416..3213
                     /locus_tag="O0286_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCY0170840.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            3353..5113
                     /locus_tag="O0286_002501"
     CDS             3353..5113
                     /locus_tag="O0286_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCY0170841.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            5114..>5877
                     /locus_tag="O0286_002502"
     CDS             5114..>5877
                     /locus_tag="O0286_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCY0170842.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPS"
BASE COUNT         2151 a          848 c         1135 g         1743 t
ORIGIN      
        1 actttagaaa atagtagaaa tagcattcaa tatagtgcaa aagtgcaaat tgataacttg
       61 acacttatct cctataaacc gtacaattaa tttgtatgat ttatatataa tttcataaag
      121 tcatattgaa tttcatataa agagcaaacc ctagaaaagg aggtgtttgt gtgaatttat
      181 taagcctcct actcattttg ctggggatca ttctaggagt tgttggaggg tatgttgttg
      241 cccgaaattt gttgcttcaa aagcaatcac aagctagaca aactgccgaa gatattgtaa
      301 atcaagcaca taaagaagct gacaatatca aaaaagagaa attacttgag gcaaaagaag
      361 aaaaccaaat cctaagagaa caaactgaag cagaactacg agaaagacgt agcgaacttc
      421 aaagacaaga aacccgactt cttcaaaaag aagaaaactt agagcgcaaa tctgatctat
      481 tagataaaaa agatgagatt ttagagcaaa aagaatcaaa aattgaagaa aaacaacaac
      541 aagtagatgc aaaagagagt agtgttcaaa cgttaataat gaagcatgaa caagaattag
      601 aacgcatctc cggtctcact caagaagaag ctattaatga gcaacttcaa agagtagagg
      661 aagaactgtc acaagatatt gcagtacttg ttaaagaaaa agaaaaagaa gctaaagaaa
      721 aagttgataa aacagcaaaa gaattattag ctacagcagt acaaagatta gcagcagatc
      781 acacaagtga atcaacggta tcagtagtta acttacctaa tgatgagatg aaaggtcgaa
      841 tcattggacg agaaggacga aacatccgca cacttgaaac tttaactggc attgatttaa
      901 ttattgatga cacaccagaa gcggttatat tatctggttt tgatccaata agaagagaaa
      961 ttgctagaac agcacttgtt aacttagtat ctgatggacg tattcatcca ggtagaattg
     1021 aagatatggt cgaaaaagct agaaaagaag tagacgatat tattagagaa gcaggtgaac
     1081 aagctacatt tgaagtgaac gcacataata tgcatcctga cttagtaaaa attgtagggc
     1141 gtttaaacta tcgtacgagt tacggtcaaa atgtacttaa acattcaatt gaagttgcgc
     1201 atcttgctag tatgttagct gctgagctag gcgaagatga gacattagcg aaacgagctg
     1261 gacttttaca tgatgttggt aaagcaattg atcatgaagt agaaggtagt catgttgaaa
     1321 tcggtgtaga attagcgaaa aaatatggtg aaaatgaaac agttattaat gcaatccatt
     1381 ctcatcatgg tgatgttgaa cctacatcta ttatatctat ccttgttgct gctgcagatg
     1441 cattgtctgc ggctcgtcca ggtgcaagaa aagaaacatt agagaattat attcgtcgat
     1501 tagaacgttt agaaacgtta tcagaaagtt atgatggtgt agaaaaagca tttgcgattc
     1561 aggcaggtag agaaatccga gtgattgtat ctcctgaaga aattgatgat ttaaaatctt
     1621 atcgattggc tagagatatt aaaaatcaga ttgaagatga attacaatat cctggtcata
     1681 tcaaggtgac agttgttcga gagactagag cagtagaata tgcgaaataa tttttgtctc
     1741 cctcacaaat tagtgaggga gcttttttaa gttgtagtct taatctagtt agacagcact
     1801 ttatcggtaa taactatatt aaacagtagt tatttgaaag taagacggac cttatattaa
     1861 ataagaagtt attgctttta ataaaaatgt tttaggcttc gtaattacta tatttatatt
     1921 atgtaaacct ataaagatga ttgcttttct atccaataaa aaagaagaga agatgtaaca
     1981 catcttctct tctgcaatat taattaggat ttatttctaa gttgagttat tttaattgta
     2041 aatctgtttt ctttaattct tttataactt ctgcagtatc ataacaattt gttgcaattg
     2101 ttgaatatct ctctgctaaa cgatatgcat taatgtaaag ctttaaactt tctttagcta
     2161 tatcctctgc atcttcgaat tttgatgggt tagacataac cactaattct gcaaattttt
     2221 ctggatcaat attaatagac atgtatttat ttacaactcc tatttatttt gatgtcttaa
     2281 tactaacata ttgaagtttt cagacaaagt aatgtctctc tataattgaa gaaaaataat
     2341 tcaaagaatt taatatttac tttaaatatt agaacgtatg aattaaacta gtatttatga
     2401 gaggatgaac aaaacatgag aataatgttt ataggggata tcgtaggtaa aattggacga
     2461 gacgcaattg aaacgtacat acctcaactg aagcaaaagt ataaaccaac agttacaatt
     2521 gtaaatgctg aaaatgcagc acatggtaaa ggtttgactg aaaaaatata taaacaatta
     2581 ctaagaaatg gtgtagattt catgactatg ggtaatcaca catatggtca acgtgaaatt
     2641 tatgatttta tagatgaagc aaaacgacta gtaagaccag cgaattttcc ggatgaagcg
     2701 ccgggaattg gtatgagatt tatacaaatt aatgatatta aacttgcagt tattaatctg
     2761 caaggaagag cgtttatgcc agatattgat gatcctttta aaaaggcaga tcaattagtc
     2821 aaggaagcac aagaacaaac tccgtttata tttgttgatt ttcatgcaga aacaacttct
     2881 gaaaagtatg caatgggatg gcatttagat ggtagagcta gcgctgttgt tggaacgcat
     2941 acacacattc aaacagcaga tgaacgtatt ttaccaaagg ggacagggta tataacggat
     3001 gttggtatga caggttttta tgatggcatt ttaggaataa ataaaacaga ggtaattgag
     3061 cgttttatca ctagtttgcc acaaagacat gttgttccaa atgaaggtag aagtgtatta
     3121 tctggtgttg ttattgattt agacaaagaa ggtaaaacaa agcacatcga acgtatattg
     3181 ataaatgatg accatccatt ttcaacattt taaaattacg taagtaaaca ttcgaattgg
     3241 accctatcgt ccattagtat gaatttaata tagtaccact gtttacatag taaatcggtg
     3301 gttctttttg ttatcattta atatgaaata tatccatagg aggcatataa ctatgaaacc
     3361 acaattatcg tggaaagttg gcggtcaaca aggcgaaggt attgaatcaa ctggggaaat
     3421 cttcgctacg gctatgaata gaaaaggata ttatttatat ggatatagac atttttcaag
     3481 tcgtatcaaa ggtggacata cgaataataa aattagagtt tctacgacgc ctgttcatgc
     3541 aattagtgat gatttagata ttttgattgc atttgaccaa gaaacaattg atgttaacca
     3601 tcatgaaatg agagaagaca gtattatttt agctgatgcc aaggctaaac ctgtgaaacc
     3661 agaaggatgt catgcacagc ttattgaatt accttttaca gcaaccgcta aagaattagg
     3721 tacagcatta atgaaaaaca tggttgcaat aggtgctact agcgcattga tgaatttgaa
     3781 tacaaataca tttgaagaac ttattactaa tatgttttct aaaaaaggtg acaaggtagt
     3841 tgaagtcaat atccaagcat taaacgaagg ttatcaatta atgcaatctc gcttacctga
     3901 aatctacggg gactttgaat tagagtcaac agatgcacta ccacatctat atatgattgg
     3961 taacgatgcc attggattag gtgcaattgc tgcaggttca caatttatgg cggcatatcc
     4021 tattacacct gcgtctgaag ttatggaata tatgattgcc aatatatcta aagtaaacgg
     4081 agcggttatt caaacagaag atgaaattgc tgctgtaact atggctattg gtgcaaatta
     4141 tggtggagtc agagcgttta cggctagtgc tggtccaggt ttatctttaa tgatggaagc
     4201 aattggatta tctggtatga ctgaaacgcc attagtcatt attaataccc aacgaggtgg
     4261 accttctact ggattaccta cgaaacaaga acagtcagat ttaatgcaaa tgatttatgg
     4321 tacacatggt gatattccaa aaattgttgt agcaccaaca gatgcagaag atgcatttta
     4381 tttaactatg gaagcattta atttagcaga acaatatcaa tgccctgtta tagttctaag
     4441 tgatttgcaa ttatctttag gtaaacaaac tgttgaaaaa ttagattata atcgtattga
     4501 aattaaacgt ggtgaaatca ttcaatctga tattgaacgt gaagaagatg ataaaggtta
     4561 tttcaagcgt tatgcgttaa catccgatgg tgtttctcct agacctatcc ccggtgttaa
     4621 aggaggtatt catcatataa ctggtgtgga acacaatgaa gaaggtaaac ctagtgaatc
     4681 tgcgtcaaat agacaacaac aaatggaaaa acgaatgcgt aaaattgagc agttactaat
     4741 tgaatcgcca gtagaagcta acttacaaca tgaggatgca gatattcttt atatcggttt
     4801 tatttctaca aaaggtgcaa ttcaagaagg tagtaaccgt ttgaatcaac aaggcataaa
     4861 agttaacact atacaaatta gacaattgca tccattccca acaagcgtta ttcaagatgc
     4921 agttaataaa gcgaagaaag tcgttgtagt ggagcacaat tatcaaggac aattggctag
     4981 tattataaaa atgaatgtca atattcatga taagattgaa aattatacaa agtatgatgg
     5041 gacacctttc ctaccacatg aaatcgaaga aaaaggcaaa ataattgcta ctgaaataaa
     5101 ggagatggta tagatggcga catttaaaga ttttagaaat aatgttaagc ctaactggtg
     5161 ccccggatgt ggcgatttct cagtacaagc tgcaattcaa aaagcagccg caaatatagg
     5221 gttagaacct gaagaagtag ctatcatcac cggtatagga tgttctggcc gtctttcagg
     5281 atatattaat tcttatggcg ttcattctat tcacggacgt gcattacctt tagctcaagg
     5341 tgtaaaaatg gcgaataaag atttaactgt tattgcatcg ggaggagatg gtgatggtta
     5401 tgctataggt atggggcata caatccatgc tttaagaaga aatatgaaca tgacgtatat
     5461 agtcatggat aatcaaattt atggtttgac aaagggacaa acatcgccgt catcagcagt
     5521 aggatttgtt actaaaacaa cgccaaaagg taatatagaa aaaaatgttg cgcctttaga
     5581 attagcatta tcatctggtg ccacatttgt agcccaaggt ttttcaagcg atattaaagg
     5641 attaacaaaa ctaattgaag atgcaattaa tcatgatgga ttttcattcg ttaatgtctt
     5701 ttcaccatgt gtgacttata ataaaattaa cacatacgat tggtttaaag aacatttaac
     5761 aagtgttgat gacattgaaa attatgattc tacagataaa caattagcga ctaaaactgt
     5821 tattgaacat gaatctttag taactggtat tgtttatcaa gataaagaaa caccatc
//
LOCUS       DAKTGS010000154         5871 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.157, whole genome shotgun sequence.
ACCESSION   DAKTGS010000154 DAKTGS010000000
VERSION     DAKTGS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.157"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            158..1393
                     /gene="arcA"
                     /locus_tag="O0286_002503"
     CDS             158..1393
                     /gene="arcA"
                     /locus_tag="O0286_002503"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0170843.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1426..2436
                     /gene="argF"
                     /locus_tag="O0286_002504"
     CDS             1426..2436
                     /gene="argF"
                     /locus_tag="O0286_002504"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0170844.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            2527..3957
                     /gene="arcD"
                     /locus_tag="O0286_002505"
     CDS             2527..3957
                     /gene="arcD"
                     /locus_tag="O0286_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0170845.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            3974..4915
                     /gene="arcC"
                     /locus_tag="O0286_002506"
     CDS             3974..4915
                     /gene="arcC"
                     /locus_tag="O0286_002506"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0170846.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            5014..5718
                     /locus_tag="O0286_002507"
     CDS             5014..5718
                     /locus_tag="O0286_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0170847.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         2076 a          815 c         1159 g         1821 t
ORIGIN      
        1 aaaatgcctt tatttatgta taaaaatcca ttattatgta ttgtataaca aaaagatatg
       61 aaattttcga ctttctttat gtgaatataa tcacatgtaa gcgtttgaag attgtctata
      121 ctctaaatga attcaaagat aaaaggagga aatagacatg acagatggtc caattaaagt
      181 aaatagcgaa attggagctt taaaaactgt gttacttaag cgtcctggaa aagaattaga
      241 aaatttagta cctgattatt tagatggatt actatttgat gatattccat atttagaagt
      301 agctcaaaaa gagcatgacc attttgcgca ggtgctaaga gaagagggtg ttgaagtact
      361 ttaccttgag aagttagcag ctgaaagtat tgaaaatcct caagtaagaa gtgaatttat
      421 tgatgatgta ttagcagagt ctaaaaaaac aatattaggt catgaagaag aaattaaggc
      481 attatttgcg acactttcta atcaagaact tgtagataaa ataatgtcag gggtacgtaa
      541 ggaagaaatt aatccgaaat gtacacatct agtagagtat atggatgata agtatccatt
      601 ctatttagat ccaatgccaa acctttattt tactagagat ccacaagcct caataggaca
      661 cggtataaca atcaatcgga tgttctggag agcacgacga cgagaatcaa tatttattca
      721 atatattgta aagcatcatc ctagatttaa agatgcgaat attccaatct ggttagatcg
      781 agattgccca ttcaatattg aaggcggcga tgaacttgtt ttatctaaag atgtcttggc
      841 tataggcgtt tcagaacgta catctgcaca agctattgaa aagttagcgc gacgtatttt
      901 tgaaaatccg caggcgacgt ttaaaaaagt agtagcaatt gaaattccaa ctagtcgaac
      961 ttttatgcac ttagatacag tatttacaat gatagattat gacaaattta caatgcattc
     1021 agccatttta aaggcagaag gcaatatgaa tatatttatt attgaatatg atgacgtaaa
     1081 taaagatatt gccatcaaac aatctagtca tttaaaagat actttagaag acgtactagg
     1141 tatagatgat atccaattca ttccaacagg aaatggtgat gtcattgatg gtgctagaga
     1201 gcaatggaat gatggctcaa atacattatg tataagacca ggcgttgtag tgacttacga
     1261 tagaaactat gtatcgaatg atttattgag acaaaaaggc attaaagtca ttgaaatatc
     1321 tggtagcgag ttggtacgtg gacgtggggg ccctagatgt atgagtcaac cattattcag
     1381 agaagacatt taacgtaata aattatagaa acgagggtga aaataatgac agaaattcaa
     1441 aaaccgtatg atttaaaagg cagatcatta ttaaaagaaa gtgattttac caaagcagaa
     1501 ttcgaaggac ttattgattt tgcaattaca ttaaaagagt ataagaaaaa cggtattaag
     1561 catcactact tatctggaaa aaatattgca ctactattcg aaaagaattc gacgagaacg
     1621 cgtgctgcgt ttacagttgc gtctattgat ttaggtgcgc atccagaatt tttaggaaaa
     1681 aatgatattc aattaggcaa aaaagaatct gtagaggata ctgcgaaagt attaggtaga
     1741 atgttcgatg gtattgaatt ccgtggtttt tcacaacaag ctgttgaaga tttagcgaag
     1801 ttctctggtg taccggtgtg gaatggatta acagacgatt ggcatcctac acaaatgtta
     1861 gctgatttta tgacaataaa agagaatttt ggatatctag aaggaataaa cttaacttac
     1921 gttggagatg gacgtaataa tattgcgcat tcattaatgg tagcaggtgc tatgttaggt
     1981 gttaatgtaa gaatttgtac acctaaatca ttaaatccaa aagaggcata tgttgatatt
     2041 gcaaaagaaa aagcgagtca atatggtggt tcagtcatga ttacggataa tattgcagaa
     2101 gcagttgaaa atacagatgc tatatataca gatgtttggg tatcgatggg tgaagaaagt
     2161 gaatttgaac aacgtattaa tttattaaaa gactatcaag tgaatcaaca gatgtttgat
     2221 ttaacaggta aagattcaac gatattctta cattgtttac cagcattcca tgatacaaat
     2281 acactttatg gacaagaaat ttatgaaaaa tatggattag ctgaaatgga agttacagac
     2341 caaatcttta gaagtgaaca ttcaaaagtg tttgatcaag ctgaaaatag aatgcataca
     2401 attaaggcag taatggcagc aacattgggg agttaatcac taaatggaac gatatgaata
     2461 tgatgtgtct gatgatataa gtgtcatgta cagacacctc atattggtat taaaggagaa
     2521 atgaatatga acgaatcagg agataacaaa ctcagtaaat cttctttaat tggactagtt
     2581 ataggatcca tgattggtgg cggtgcgttc aatataatgt ctgatatggg cggtaaagcc
     2641 ggtggattag ccattattat tggttggatt attacagcta taggaatgat ttcattagcg
     2701 ttcgtatttc aaaatttaac caatgaacgg ccggagctag acggtggtat ttatagttat
     2761 gctcaagcag gatttggcga ttttgtagga tttatcagtg cttggggata ttggttctca
     2821 gcgtttttag gcaatgttgc ctatgcaaca ctattgatgt cagcagtagg taactttttc
     2881 ccgattttta aaggaggcaa cacattacca agtgttattg tcgcctcgtt actactctgg
     2941 ggtgtccatt tcttgatttt aaaaggcgtt gaaacagcag catttatcaa tagtattgtt
     3001 actgttgcaa agttaatacc gattttactt gtaatcatat gcatgataat tgcattcaat
     3061 tttgacactt ttaaaacagg ctttttcagt atgacgtcag agggtgtatt gccatttagt
     3121 tgggcgagca caatgagcca agttaaaagt acgatgctag tgacagtttg ggtgtttatc
     3181 ggtatcgaag gtgcagtaat tttttctagt agagctaaaa ataaaaaaga tgtaggtagt
     3241 gccacggtta taggacttat atcagtttta attatctatt tcttattaac tgtattagct
     3301 caaggcgtga ttttgcaaaa tcatatttcg caattagatt cgccaagtat ggcacaggtg
     3361 cttgcaacta ttgtaggtgg ttggggatct acacttgtaa atattggttt aattatttcg
     3421 gtactaggtg catggttagg atggacactg cttgctggtg aattaccttt cattgttgca
     3481 aaagatggat tatttccaaa atggtttgct aaagaaaata aaaatggagc acctgtaaat
     3541 gcactgctta ttaccaatat attagtacaa ttatttttaa taagtatgct atttacacag
     3601 agtgcgtatc aatttgcatt ttcactagca tcaagtgcta ttttataccc ttacatgttc
     3661 agtgcatttt accaagttaa atacacttta gagcatcgac agcaagcaac tactaaacaa
     3721 tggacgattg gtatcatagc ctcaatttat gctatatggc ttatatatgc agcaggtatc
     3781 aattacttat tattgacgat gttactttat attccagctc ttcttgttta tacaatcgtt
     3841 caaaagaata atcagacacg tttgattaaa tcagactata ttctttttat gattattatc
     3901 gtacttgcag ttatcgggtt aattaagtta ttgatgggaa cgataaatgt tttttaaaag
     3961 gagcgacaaa aatatgaaag agaaaattgt cattgcatta ggcggtaatg cgatacagac
     4021 aacagaagca acagctgaag cacaacaaac agctattaga tgtgcgatgc aaaaccttaa
     4081 acctttattt gattcaccag cgcgtattgt catttcacat ggtaatggtc cacaaattgg
     4141 aagtttatta atccaacaag ctaaatcgaa cagtgacaca acgccggcaa tgccattgga
     4201 tacttgtggt gcaatgtcac agggtatgat aggctattgg ttggaaactg aaatcaatcg
     4261 cattttaact gaaatgaata gtgatagaac tgtaggcaca atcgttacac gtgtggaagt
     4321 agataaagat gatccacgat ttgataaccc aactaaacca attggtcctt tttatacgaa
     4381 agaagaagtt gaagaattac aaaaagaaca gccagactca gtctttaaag aagatgcagg
     4441 acgtggttat agaaaagtag ttgcgtcacc actacctcaa tctatactag aacaccagtt
     4501 aattcgaact ttagcagacg gtaaaaatat tgtcattgca tgcggtggtg gcggtattcc
     4561 agttataaaa aaagaaaata cctatgaagg tgttgaagcg gttatagata aagattttgc
     4621 tagtgagaaa ttagcaacgc tgattgaagc agatacctta atgattctta cgaatgtaga
     4681 aaatgtattt attaacttta atgaacctaa tcaacaacaa atcgatgata ttgatgtagc
     4741 aacactgaaa aaatacgcgg cacaaggtaa gtttgtggaa ggatcgatgt tgccaaaaat
     4801 agaagctgcg atacgatttg ttgaaagtgg ggaaaacaaa aaagttatca ttaccaattt
     4861 agagcaggca tacgaagctt tgattggtaa taaaggtaca cacattcaca tgtagcactt
     4921 tatcacgcga caaaacatta aatatgtttc tccgttgatt caaatgaaaa agttgtctgc
     4981 tgacactttg caaggtttga aggagtttaa cttatgacag aaaactttat tttgggtaga
     5041 aataataaat tagaacatga actaaaggca ttagcagatt acattaatat accatatagt
     5101 atattacaac catatcaaag tgaatgtttt gtcagacatt atacgaaagg ccaagttatt
     5161 tatttttcgc cacaagaaag tagcaatatt tactttttaa ttgaaggtaa cattattaga
     5221 gaacattaca atcaaaatgg agatgtatat cgttatttta ataaagagca agtattattt
     5281 ccaatcagta acttatttca tccgaaagag gttaacgaat tgtgtacagc attaaccgat
     5341 tgtacagttc ttggattgcc tagagaattg atggcctttt tgtgcaaagc taatgatgat
     5401 atatttttga cactttttgc attaataaat gataatgagc agcaacacat gaactataac
     5461 atggcattaa caagtaaatt tgctaaagat cgaattatca aattgatatg ccatctatgt
     5521 cagacagtag gatacgatca agatgaattt tatgaaatca aacagttttt aactattcaa
     5581 ctcatgagtg atatggctgg gatttcccgg gaaacagctg gtcatattat tcatgaactt
     5641 aaagatgaaa aacttgttgt taaagatcat aaaaattggt tagtaagcaa acatttattc
     5701 aatgatgtat gtgtttaata tacaatgtaa aatgaataag ttgaacatga ggtctaacgt
     5761 acatttatac gttaggcctt ttttgctagc atgatgaata atttaaaatg ttagttaaat
     5821 ttgattgttg aaattacagt aaaatttaag gtgatgaaaa atttagaact t
//
LOCUS       DAKTGS010000155         5783 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.158, whole genome shotgun sequence.
ACCESSION   DAKTGS010000155 DAKTGS010000000
VERSION     DAKTGS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.158"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            173..1177
                     /gene="aaa"
                     /locus_tag="O0286_002508"
     CDS             173..1177
                     /gene="aaa"
                     /locus_tag="O0286_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCY0170848.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(1362..1631)
                     /locus_tag="O0286_002509"
     CDS             complement(1362..1631)
                     /locus_tag="O0286_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170849.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            1780..2175
                     /locus_tag="O0286_002510"
     CDS             1780..2175
                     /locus_tag="O0286_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0170850.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            2165..2650
                     /locus_tag="O0286_002511"
     CDS             2165..2650
                     /locus_tag="O0286_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170851.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(2819..3601)
                     /locus_tag="O0286_002512"
     CDS             complement(2819..3601)
                     /locus_tag="O0286_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0170852.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(3598..4716)
                     /locus_tag="O0286_002513"
     CDS             complement(3598..4716)
                     /locus_tag="O0286_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0170853.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(4831..5715)
                     /locus_tag="O0286_002514"
     CDS             complement(4831..5715)
                     /locus_tag="O0286_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0170854.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
BASE COUNT         1976 a          895 c         1042 g         1870 t
ORIGIN      
        1 aataatttta aggggtaatt ataaaatttg atgatacagt atatgatttt tttgtaatca
       61 taatgtcatc aaacatcaac ctattataca taataaaatc gtataatgat gtagtattca
      121 taaattcgga taaaagaatg ttaggaaagt taagcaagag gaggatttta aagtgcaaaa
      181 aaaagtaatt gcagctatta ttgggacaag cgcgattagc gctgttgcgg caactcaagc
      241 aaatgcggct acaactcaca cagtaaaacc gggtgaatca gtgtgggcaa tttcaaataa
      301 gtatgggatt tcgattgcta aattaaagtc attaaacaat ttaacatcta atctaatttt
      361 cccaaaccaa gtactaaaag tatctggctc aagtaattct acgagtaata gtagccgtcc
      421 atcaacgaac tcaggtggcg gatcatacta cacagtacaa gcaggcgact cattatcatt
      481 aatcgcatca aaatatggta caacttacca aaacattatg cgacttaatg gtttaaataa
      541 tttctttatt tatccaggtc aaaaattaaa agtatcaggt actgctagct caagtaacgc
      601 tgcgagcaat agtagccgtc catcaacgaa ctcaggtggc ggatcatact atacagtaca
      661 agcaggtgac tcattgtcat taattgcatc aaaatatggt acaacttatc aaaaaattat
      721 gagcttaaat ggcttaaata atttctttat atatccgggt caaaaattga aagtaactgg
      781 taatgcatct acgaactcag gatctgcaac aacgacaaat agaggttaca atacaccagt
      841 attcagtcac caaaacttat atacatgggg tcaatgtaca tatcatgtat ttaatcgtcg
      901 tgctgaaatt ggtaaaggta ttagtactta ttggtggaat gctaataact gggataacgc
      961 agcggcagca gatggttaca ctatcgacaa tagacctact gtaggttcta tcgctcaaac
     1021 agatgtaggt tactatggtc atgttatgtt tgtagaacgt gtaaataacg atggtagtat
     1081 tttagtttca gaaatgaact attcagctgc accaggtatt ttaacttaca gaacggtacc
     1141 agcttaccaa gtaaataatt atagatatat tcactaaagt cttacgtata taaatatata
     1201 atgaattcct attacatttc acaagctgaa aagttggatt gaatcctaaa ttttatggcg
     1261 gggctatcaa agtcgtgatt gttgtaatag gaatttttgt atatgaaaaa ggattggtcg
     1321 aatgaacttc atgttctatg ttcgaccaat ccttttctga attagtattc atgttttact
     1381 ttgcgaatgc ttgtaaataa tctagcaccg tttgttatta aagtaacaac tgccactgct
     1441 tttttgaact tacgtggtga cttaattgaa atgtaaaagt ctaacacggt tcctacagtg
     1501 cttaaaccta atgaaagata tactaatttt ttatttttag catgatattt atagccattg
     1561 tagccgtcga ctatgaaacc tgcgacattt agtaaacttg ataaacgttg tgatttggaa
     1621 cgttttgcca taataataat tccccctagt atcttctgtt tatatgaaaa gttgggtgcg
     1681 ctgaattgct aacgttttgc gctataacta ctcatatatg ataacataat tgtacagtat
     1741 aatttgaaaa attgatttca caaagttggg gtgtcaaaga tgattaaatg tgtctgttta
     1801 gttgaagaaa cagctgataa aatattgctt gttcaagtaa ggaatcgcga aaagtattat
     1861 ttcccgggtg gtaaaataga agaaggggaa tcacaagtac acgcgctgtt aagagaagta
     1921 aaagaagaat taaatttaac attaacaatg gatgaaattg aatatgtcgg gacaattgta
     1981 ggtcctgcat atccacaaca ggatatgtta actgagttaa atggatttcg cgcattaacc
     2041 aaaatcgatt gggaaaacgt aactatcaat aatgaaatta cggatatacg ctggattgat
     2101 aaagataatg atgcgttgat tgcgcctgct gtcaaagttt ggattgaaac ttatggtggt
     2161 aaacatgaca aataatgaca ccatcatgtt acgacattat gtcccacaag attattcgat
     2221 gttagaagct tttcaattaa gtgaaagtga tttgaagttt gttaaaacgc cagaggaaaa
     2281 tattacagct gcaatgtctg ataatgaaag gtatcccatc gttgtaatgg atggcaggca
     2341 atgtgtggcc ttttttacat tacatcgtgg aaaaggggtc gcaccattta gcgataacca
     2401 agatgcagta tttttcaggt catttagtgt tgatcaacgt tatcgtaata gaggaatagg
     2461 taaagtggta atggaaaaat tggcgtcatt tatcacttca acatttcagg atattaatga
     2521 gattgtgtta acggttaata ctgacaatcc acatgccatg gcactttatc gccaacaagg
     2581 atatcaatat atgggagata gtatgttcgt cggaagacct gttcatatta tggcgttaac
     2641 tataaaataa attaaattta aaagcatctt tactcatcgt cgaccacaac aattaatgat
     2701 gaataaaggt gctttttgtt atagatcatc ggacaattta ctatagtaaa aagcgaccta
     2761 gtgaacaatt gacatatatc cacaggtcgc ttaacttaag ttatattgct agttgcgatt
     2821 aattgataga ctcatcattt ttgcgctgtc gagatggtct ttttattaaa aatgccgtaa
     2881 tccaagccgt aatcggaata ctgattgcaa cggcaatacc gcctaaaata atagaaataa
     2941 attcttgggc aaatattttc gagtttataa tatgaccaaa tgaatattta agtttgaaaa
     3001 accaaataaa taaagcaagt tggccaccaa aaaaggcaag gtaaatcgtg ttcgcagatg
     3061 tcgctaaaat ttctctacca acacgcatgc cagattggaa taattcgtat tgcgtaagcg
     3121 ttggattcac ttgatgcaat tcataaatgg gtgaactaat ggtaattgtt aaatctatca
     3181 cagctgcaat aacagcaaga ataatagtga acaccataaa ttgaaccata tcaatgccaa
     3241 tattcattga atacacatat gtttcatctt gttgttcggt tgaaaagcct tgtagatgac
     3301 cgaagtagac cgataaataa atgagtgtaa tcaacaatat tgttgtaacg atagtgctga
     3361 taaatgcagc ttgtgtttta acattgtaac tattgagtac gaataaatta caagcgccaa
     3421 taataatgca gaaaaagaat gtgacgacat aaatcggtac gccaaaaata atcaatacaa
     3481 tactaataat taaaatagcg aaatttaaaa atagggttaa ataagagatg aatccctttt
     3541 tacctccgaa aattatcatc agaaagagga gcaataacgc caatataaat acagcattca
     3601 ttgtttcgcc ctccttaatg tttcaaatat ttccataaac aatattgtga taggaattgt
     3661 aagtacgata cctataccac ctgttagtgc gcgcgcaatt tctaacgacc aattcatcga
     3721 aatagtataa gtcactgtat tggcattttt taaaaagatt aaaaacatag gtagtgcacc
     3781 ggataaatat gagaataata agatgttagt cattgttccc ataatatctt ggccgatgtt
     3841 tcgcccagca agcgcccatc tcctcattga aatgtgtggc gtacgctgta aaatttcatg
     3901 cataccacta gcaattgtaa ttgcaacatc cataatagcg ccaagtgaac ctattaacac
     3961 tgaggctagg aagatatctt tcggtggtaa tgataaaaag ttcatcgttt catatttaat
     4021 gcctttacca tctgccatat atatgattaa ttctgttaaa cctatactca aaaaagttcc
     4081 gataattgta ctggctatgg taatgagtgt acgcatatgc cagcctgtaa cgagcaataa
     4141 agtgagtatt gttgaacaga tcatggcaat ggtcatgagt aagaataaat taatattgct
     4201 atgttgaata tgaatgtaaa ttgcgattaa tatggcaata gaattcaaga ttaacgataa
     4261 aatcgattgc agtccgactt tgcgaccaac caataataca gttaataaga acaaaccagt
     4321 gatgataacc gttaaggtat cacgcttctt ttctataata taagcatcac tcggcttgtt
     4381 agaaatatgt aataatactt tttcgtgtgt gcgaaatgcc tcagaatctg cttgcgattt
     4441 gacgtactga tgattaatcg tcgtcgtttc tccagcaaat tgaccattta atattttgac
     4501 ttttaattga tttttatatt taatatcacg attattttgt gcatcttttg taggtgtcga
     4561 agaaacatgt ttgacatcta taatttgacc aattggtttg ttgtaaaagt tctcattatt
     4621 gaatgtaaat aaaatagcac caatgaatgc gatgcagaac aaacctaaaa ttatattaaa
     4681 tggctttgta aataaatttc tatatttcaa aaacaaaacc ccaattctat gaatgaatta
     4741 atatggtgat tatacgccct taatttttta ttttcaaaga tattactgct aagtgtaaaa
     4801 cgaaaatcat cattgatagc atcgaattac ttaatggaat gtagacgttt tagtcattaa
     4861 ttgctgaata agtgttaata atatgccaat atcactcttt gtataaggct cctttgtaat
     4921 agcacatatc gttcttttta attcagtatg atctaatttt atatctatcc atgatttaga
     4981 ttctggtaaa tgtatatttt gtgatgaaat gatgtaacct tctttttgac gaaggagata
     5041 ctgcgcaagt ggttggctac tgattgtgta tacatctgat ttagtaatct tgcgcaattg
     5101 tttttttaca gtttcggcaa atggtgccaa gcaataaata tgactatgct caaactgaat
     5161 taatggtggg tgtgtcgcca tcgtaattgg atcgtctgaa ggcgcatata aatgatagtg
     5221 ctcttcgaat aaaggtagca tatgtaattg tttgtgttta cgtatttctg gtgtaagttc
     5281 cgtgaaacca atgtctatat tcccatttaa tacgctattt ataattgtgt catgttctaa
     5341 taagctcggt atgacatgtg tatcattttg taaatgaaac gtttggataa gtggtagtaa
     5401 catgtgggat acgtcactct catcatagcc aatgtagata cttttatttt tagttaatcc
     5461 atggctttga aattgttcaa tcgtgctatc taaatgttca ataatacgca gagcttcatt
     5521 aaataataat ttcccttcag atgtgagcgt aatattgcgt ccttgctttt taaataaaga
     5581 cacattaagt tcttgttcta ataatgtaat ttgacggctt atcgctgatt gagcaatgtt
     5641 tagttcaagt gctgtttcgg agatatgttc tcttttagcg acctcgataa aatatcttaa
     5701 ttgtttaatt tccatagcga tataggcacc tccaaaaatg agtgttttgt aactattata
     5761 gcaatattat tgataaatgt tct
//
LOCUS       DAKTGS010000156         5743 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.159, whole genome shotgun sequence.
ACCESSION   DAKTGS010000156 DAKTGS010000000
VERSION     DAKTGS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.159"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..3431)
                     /locus_tag="O0286_002515"
     CDS             complement(<1..3431)
                     /locus_tag="O0286_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCY0170855.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKT"
     gene            3735..5075
                     /locus_tag="O0286_002516"
     CDS             3735..5075
                     /locus_tag="O0286_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0170856.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(5281..5613)
                     /locus_tag="O0286_002517"
     CDS             complement(5281..5613)
                     /locus_tag="O0286_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0170857.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT         1796 a          926 c          844 g         2177 t
ORIGIN      
        1 atagttttca gttgttgatg tttatctttc aattgattga taagtgttgt atctaaatca
       61 aatgctaata aatcgtttat ttgcgtttga atattagctt caaaactttc aaattgcgat
      121 tcagcttcta cagccatttg ctttacatat aattcaagct gttgcctcaa taatcccatt
      181 tgtggttgta ataattctaa cgtactttga caaattgatt catgtatatc tctttgccca
      241 tttggattca aaattttacg ttttgttaat tgtttaggca atgcatttag catatcgttg
      301 aaatcgatgt gcaataatgg ttgctctata ttagctgatt caaagttaaa ctgaggatta
      361 ataatgacat gtaaatctgc taattgttga acgattggtg caatttgctc agtgagttgc
      421 ttattaaagt atttttttat acgttctgta attaaagatt gctctaaaaa caatcgttga
      481 tgaatttgat ctaagtatac tctcgtagac actttctttt cttcattaaa atcactattt
      541 tgcgtcattt gagaattaaa cactgattta acatcgtcaa gtagttgtag ttttaaacgg
      601 gcatttaaat gataaacttg ttcttcaact tcgttgtctg tatgttgtaa cgtcgcatca
      661 attaattgat gttgtaaacg ctgtttatct ttataatctg ttaacctttg ttggcgacgt
      721 gaaatatcag ctttatttgt ttcaaattct gtaatcatct ctacataaga acgatccatt
      781 tgttgaagct gatgaatcat ttgttgttct aaaattgatt tagattcaac atcaacaaat
      841 tgttgtatgc tttgttttaa ttgatcaatg cccttatctt cagcttgcaa tgcatttcga
      901 cttgatacag caaaaatgtc tgattgtaag tgtacttgtc ttaatgcatc tgatacatat
      961 gtttcaactg cttcaagatc atcttgactt tctgctaaat cagcagcatt aattaccatt
     1021 tttaatgctt ggttttcatt caactggttc atatctttca tgtgttctat aaacgctttg
     1081 tcattatcag taaatgaatg attaaaataa cttacataca atattaagtc tgaagaagtt
     1141 aaaatttgtt cggtttcatt tgtatgcctt tggttatttg agtgtagccc taatgaatca
     1201 acaattattt taccttttaa ccaatcatgc attaatgcaa tgtgtactgt cttaacaaat
     1261 gttgcatatt catcttctgc actccacttt ttcaattcct gttgattaat ggcatgtttt
     1321 tctccatttt ccaacatatt gacatacaat ttataatgtt tttcaactgc ctgaataaaa
     1381 gcgagttgat ttttatttaa atgtgatttt aacttttcta aatctgaatt aataaagtct
     1441 tctatagtcg aataagacat atcttggtat tcaactactg cattaatttc atctaataat
     1501 tgcgattgtg attttagcgt tatataactc tcgtctccat aagatatttc tgtagtagct
     1561 gctgtagtag gatttggaga actgactaaa atatgctcgc ctaataatgc atttatcaaa
     1621 ctacttttac cagcactaaa tgttccaaat acacctattt ttattaattt attatctata
     1681 cgtgttaatg tttcgtggat atcttgcttt gtacgcttga acaaaggcac atccgaaatt
     1741 atatcaagcg ctttttgaat atctatagac atatttgttg tagctgtatt ttgatttagc
     1801 tgctgattat cacgattatc ctgagcatta tcagtagcca cttgatatgt tgtctcttgt
     1861 cgacctatta atttatctag agattcatct aaatgaatat agtagtgacg ataattctta
     1921 gtcgtcaatg actgacgcag ctcatttaat tctgtataac gttgatattc ttttaaatca
     1981 tcactttctt ctgttggtaa ttcatctgcc tgcacatttt ctattatttc tttaaaaatt
     2041 ggtgttgatt gttgttcaac atatttctta atggctttaa ccacttcgtc tgaaaatgta
     2101 agtacataag tattgctgat tgatgtttgt ggttgatata aaccttcaat catttctggc
     2161 ttaacgtcat aatgctgatt taatacttta tctgaagctt cttttttatt gataaaacgc
     2221 gtaacaaatg acatatcttc tcgcattggt tgacgaattt gttgattaac atgttcttgt
     2281 aacgcatctg tcgctgttaa taatcgctgt tgttgaattt gtagtttttt cttcttttta
     2341 ttaaaaaatc cgccaacatt aaagtcttga gacatacttt ccaaataact tcgtaacatt
     2401 tctctcatat tatgcggcat aatgtatgca ttttctaaaa tatttttacg cttattcttt
     2461 aaaaatgcca ttaattcatc tggattattt aaaagttgtg cctcttcact aattgcttga
     2521 tgttgttgac tatttaaaaa tgcttgttca aactccgctt cttcgatacc taaatcttct
     2581 agtacttcct gaatttcaga ctgaatgtaa tctagctgag cttcggtaat gtattcaacc
     2641 gttcttgatg tataatcctc tattgtctct ctatgttgat ctaatgaaat tagataactt
     2701 gataatactt caagttcatt ttcagggtga tcaaatttag atacataaaa ggtgcgttct
     2761 aatttaatac cccaatctgc aattgatttt tcaactcgag atttaaacgt agagaatgac
     2821 aattcatcgt cttgatgctt gtcaatttga ttaatgataa acacaacagg tattccaaca
     2881 tcatttatat gcttcataaa cttaaagtta agttcagatt gaacgtggtt atagtcaacc
     2941 gtataaaata tcatattact tgtatacata tattgttctg ttattgactg atgtgatgca
     3001 acatttgaat caacacctgg tgtatcttgc aacgtaaacc cattttcaaa tttagctgat
     3061 tgaaaattaa tttctacaga ttcaacgtcg acattttggc gattcatttc ccttacttca
     3121 tcataattag ataatttggc atacgtttga ttcggcaaat tagcaataat atcgtgattg
     3181 tctgaaactg acacaatagc agtattactt gtcgttggta caggtgaact tggtaagata
     3241 tcttgttcaa ttaataaatt tatcagtgtc gatttacctg cagaaaaatg tccaacgaac
     3301 gaacatgtat attgctgcaa atatactttc ttaattactt ggttaattgt atgtaaaagt
     3361 gcttcatttc gcgacttttc aacttctttt tttaatttat ataaaagatc taattgttct
     3421 ttattaatca tgttactttc ccctttggtt cgttcacata gaaatgcttg cttgttacaa
     3481 cgaattcata caatatcgat gtccatttaa acatagatcg ttaaatggct cgcattgtat
     3541 tatgtttaaa ttttatcata gctgcttgat aaaacatacg aataatcaat tttcttcaaa
     3601 cattccaact taaaagttca tttttctaaa aatcacaaat catcgttaaa tttttctaga
     3661 aaataaatat tgtcacagtt tgttcacaca catcacagaa ttgacaccgt ataatagcca
     3721 attgggaggg aaatatgaaa aatacattta atccattaca aagattacat ttctatgcag
     3781 caatatttat tgctccactg ttaatcactt taaccatttc gggcattggt tacttgttct
     3841 ttccagaagt tgaaaataat atttataaga atgagttttt tggtgacagt gatgtaaaaa
     3901 cgcatcaaac attaaatgat gcagtacatc aagttgaaca acaatatgaa ggattctttg
     3961 taagtaaagt tagcatactt gatgaaccat ataacaaacg aattacactg agtgatatgg
     4021 caggaaatca acgttacgtc tttctagatc ataacaatca atttgttgca gatcaaaatg
     4081 cgaaacatac gtattctaat gtgatgcgaa gtatacatag ttctttgttt actgaaaata
     4141 ctattattaa ttatttagta gagttaaccg catgttggat gatattcatg attttatctg
     4201 gtacttattt actcattaag aagcatttaa tttctaacaa aagtaaggca cttcgttggc
     4261 aaaagtggca cgcaatgatt ggagttatca ttgcaattcc agtatttgta ttagtcttaa
     4321 ctggattgcc atggtctggt tttatgggca gtaaaattgc cggtatgatg gacacaaacg
     4381 gtgcccttgg tcaaggtgaa ttagcgatta atccacctaa atcagatttg aacgaattac
     4441 cttgggctac acgtaaaaat aaacagccag cttcatccga aaaaggttca agtggtcatc
     4501 atggtaatgc agcaatgcct caaaccaaat tagattatca aatatctatt gataaggtcg
     4561 ttgaacaggc gcaaaaagct ggtattaaaa agccgttttc aatcgtatat ccaagtgata
     4621 aaaatggtac ctttattgta tctaatacta gtaattcagg tgttactggg ctagatgtat
     4681 caccatacaa ggaacaaaca ctttatttcg atcaatatag cggtaaaaag ctaggtacga
     4741 ttaaatatga tgactacggt attattgcta aatggtttac atggggcatt ccgcttcacg
     4801 aaggtcattt attcggcatt ttaaataaaa tcattaattt atttgtatgt atcgctttat
     4861 tagtagccat tggcatgggg tttgtctctt ggataaagcg tacaaaaaat actgcaataa
     4921 aagtaccaca tcgcgtaaaa aaaccagcat ctatatcact cataatatgt ttaattgtat
     4981 taggattatt aatgccatta tttggattat cacttatcct tgtatttata attgaattaa
     5041 tattatatat taaagatcgt cgtgctaaac aataatgcac ttaaagtttt gaactgacga
     5101 aatttacaaa atgattctcg tctctctaat tacttaaaac gggttctata ataaatcgta
     5161 ctgatggaaa gtttttactt tttatctgtc cgattttttg aatttgaaga taaaaaaaca
     5221 tctaaaacgc ataagaagat aactctactt cttcaaaatt acgttataga tgctttttta
     5281 ttaattcata tgcatgtcgt gaattttcat cattaaaccg tgtgggacgt catcatgttt
     5341 aacaatctct tgtttataaa atgtcttccc atcatcttta gaaactaatt ttacaaagtc
     5401 tccttttttc ggcttgaaat cattagatgg ttctactttg atattatgtt tgactaatcc
     5461 atctttttca ctgacaactt tttctgcatt tgtattacta ttcatataac caaaataagt
     5521 cgttttttga ctagaaaaaa tcattaaaca tacaacggca ataccaataa caaccaatgc
     5581 aatagataaa cctagaccca tttttttacc catttataga ctccttcatt tttcaaaaat
     5641 acctattatc atgagaactt aattattact aattttagtc acgcattaga aacactttag
     5701 ttttcaatag ttttacgttc tgtcttttta ccccagtatt gat
//
LOCUS       DAKTGS010000157         5721 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.160, whole genome shotgun sequence.
ACCESSION   DAKTGS010000157 DAKTGS010000000
VERSION     DAKTGS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.160"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            427..1194
                     /locus_tag="O0286_002518"
     CDS             427..1194
                     /locus_tag="O0286_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0170858.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(1242..1715)
                     /locus_tag="O0286_002519"
     CDS             complement(1242..1715)
                     /locus_tag="O0286_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCY0170859.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(1839..2540)
                     /locus_tag="O0286_002520"
     CDS             complement(1839..2540)
                     /locus_tag="O0286_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170860.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(2533..3306)
                     /locus_tag="O0286_002521"
     CDS             complement(2533..3306)
                     /locus_tag="O0286_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170861.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(3293..4123)
                     /locus_tag="O0286_002522"
     CDS             complement(3293..4123)
                     /locus_tag="O0286_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170862.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(4116..5102)
                     /locus_tag="O0286_002523"
     CDS             complement(4116..5102)
                     /locus_tag="O0286_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170863.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            complement(5273..5458)
                     /locus_tag="O0286_002524"
     CDS             complement(5273..5458)
                     /locus_tag="O0286_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170864.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            complement(5406..>5721)
                     /locus_tag="O0286_002525"
     CDS             complement(5406..>5721)
                     /locus_tag="O0286_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170865.1"
                     /translation="WITTLIMILLTIFCCLVLNKWFVSAVITFVILGVLAFFIPNFQD
                     IKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYGYEGAELRRKD
                     KK"
BASE COUNT         2017 a          973 c          795 g         1936 t
ORIGIN      
        1 atgtgtgtgt ttgcaagtgt gtgatatcaa atgtcatatt tgtctaattg taatttataa
       61 aatgcatgag caatgattgc tgtaacaaat tctagcaaag tattcagttt gtattttaga
      121 aataaaaatg atgatagatg tatgatcgat ataaaagatt taatatgcac caaaatgata
      181 acgtgagtaa ttggtttaat gaaacataac atgaatgtaa atttagctta ctgacataaa
      241 cactacacaa acatacagtt atataagtcc ccttatggaa tttcacattc tagtttacat
      301 aatatatatt ataggaagtt atatgtgtgt aacgcaaaag gtaccctaca tcataatcat
      361 tatctaatat cgtcacataa cttacttatg ctataatcat ggtattatat tgtttggagt
      421 gatttgatga gatttgtctt tgatattgat ggtacgcttt gttttgacgg ccgattaatt
      481 gaccagacta ttattgatac attgttacaa ttacaacatg atggtcatga acttatattt
      541 gcatcagcac gtccgattcg tgatttgttg ccagttttac catcagtatt tcatcagcac
      601 acattaattg gcgcaaatgg tgctatgatt tcacagcaat caaagatttc tgttatcaaa
      661 ccaattcata ctgatacata tcatcatatc ttcaaaataa ttcaaaagta tgagttagat
      721 tatattattg atgatgattg gaattatgct gcacaacttg acgctgagaa cgcgattttt
      781 gagcgtttag atccacataa gctggccagt tgtattgatg ttgcaaatat cgacacacca
      841 atcaagatta ttttattaaa tatagacccg gcacaaatta caactatatt agacgagcta
      901 gataaatacc atcaagaatt ggaaatgatt caccattcaa atgagtataa cattgatata
      961 acagcgcaaa atattaacaa atatactgca ttacaatata tatttgatgc agatgttaaa
     1021 tatatagcat ttggtaatga ccacaatgat attgtcatgt tacaacatgc tagtagtggc
     1081 tatattatag gaccatcaga agcatacaca cacgcaatat tgaaacttga taaaatcaaa
     1141 cacatcaata ataatgcaca agctatttgc aaagtcttaa aatcatataa ataaaaacac
     1201 ccctatcaaa tgataatcat tatcaatcga taggggctat tttaataaaa ttcgtcctcg
     1261 aacatttctt cctcttcatc taatccaaat aattctgcca tttctccatg ttcaattaac
     1321 atgtttaaat atgcatcgcg gagttcttct tcactcatat cattaatcat ttctttaaga
     1381 ctatcaatcc acatatttct gcgtaattga tagtcttctt caacttcgtt taacatcatt
     1441 atatgtttat ttgctgcttc tggactagct gtaaagagta atgcaatcat atgtttacat
     1501 atcactcgtc ttccatcagc atgaggacaa ttacatatgg attttctagg atgttccata
     1561 tcaatataac aacgatatac tttgttgcca ctgcccttta cttcagcctc atgctgcgtt
     1621 tctgaaaatg attttaagtt aatgacgcat tcactttgat aataattaaa gcctctttct
     1681 atagaacgaa tacttgcaat atcaagtaat cccattaatg atactccttt ttattattat
     1741 ttttaaataa agaaaataaa atagataagt gtctagatta aaatacttga tttatctata
     1801 ttttataaca agtctagaat tatcgcattc ttaaataact aatatgaaaa tgcttgcact
     1861 aattcttttg tataagggtg tctatcaaca ttaaataatt cctctattgc aaaatcatcg
     1921 actatcatgc catccttaag aacgataatt ctattaacta agcgttgtaa cacggataaa
     1981 tcatgagaaa taacgataaa atgatttaag ttcgtaatcg tttgcgcttt taatatattg
     2041 attacatttt gttcagctat aacatctaaa tttgaagtta tctcatcaca tattaaaacg
     2101 cgaggctgtg ctaataacga acgcatgaca ttaaatcttt gtaattgtcc gccactcact
     2161 tcgcttggta atttagtcaa taattgcgcg tttaactcaa aagtagataa atgttgtaat
     2221 aataattgat cctgagcagt attatcagtt agacctctgt aataatataa cgcttctttt
     2281 aatgaggtct caatcgtcca atcagggtta aagctagtta aagggtgttg gaaaatcggt
     2341 aacacagcat tgtcacttaa gtaaatctct cctttaacag gtttaaacaa gccaagaacc
     2401 aatgaagcga gcgtactttt accacagcca ctttcgccta aaataccaac attttctcca
     2461 tcaggtatag taatattgat atcttgtagc accatctgct ttttattata accaaaagtc
     2521 acatgtttta actcaatcat agtaatccct ctttaattgt gttctatatt taattagacg
     2581 ttcagtatac ggatgcaaat gctcatactt gaaatgatta atattacctc gttcaatgat
     2641 ttgaccttct tttaaaacat aaatgtactg acaatatttc aatacatgac ttaagttatg
     2701 tgtgataata aataatgttt gaccatgttc taatacaata tgctgtaata aatccatcac
     2761 ttgattaccg ttcaaagcat ccaatgatgc aactggttcg tctgcaatga ttaatttagg
     2821 ctccaacatg agaacgcttg ctatgtatac gcgttcaagt tggcccccag aaagttggaa
     2881 actatattta tttaatatat ctttgctttg taaattaacc cacgacaaag ccttatcaac
     2941 tttggacaaa gcctcttctt tactaccttt ataatgctta cgataaatcg cagttaactg
     3001 tttacctaat ttagtatggt cgttaaaact ttctgcataa ttttgagaaa tatagccaat
     3061 tgtatgacca taatattgac tcaatctact aacattttcc ccatcaaatt ggtacgaatc
     3121 atacgtgcag cttaaatcaa atggtaaata ttcaagtaaa gctttagcaa tcaaactttt
     3181 tccagcgccg ctctctccaa tcaaggcatt aatctgttga ctaaaaattt tcaaatcaat
     3241 ccctttaata agagatttct cactagtatt ctttattgtt aaattttgta tatcaatgag
     3301 actcatcata ttcaccccgt tgtttcagca atctatctct tagtgcatca ccggttaaat
     3361 taaaaattaa aatagttata gcaatgactg aagcaggtgc aatcaacata attggatgag
     3421 acgaaataaa atcacgacct tgttgcaaca tagcgcccca ctctggtgtt ggcggttgtg
     3481 cacctaaccc aataaatgat agtgaactta tatatagaat gattttaccg aaatcaacga
     3541 ccatcaaaac gataatagcc ggtataattt taggtgttaa atgacgtatt aatattgttc
     3601 ttgttggtac atgaaataat tgtgccattt ttatataagg cttattcatt tcgctattaa
     3661 ctatacttct agtcaacctt gtgtaattca tccattttat taatgtaatt gagataacta
     3721 aattccataa agatggttga aaaaaacttg ctaaagcaat catgatgata aattctggaa
     3781 tacttagacc aacatcaata aaccttaaca ctaatcgttc aatccaccct tttttgtatc
     3841 cggcaaatag acctagtgta acacctatga caacgatagc tattaatgtt aaaacagtaa
     3901 caaacaatgt tgaacgtgca ccgataataa ttcgggtaaa taaatctctc ccataatcat
     3961 cagttcctaa taaatgcaac caactaatag gttcaaaagt ttgtgataaa ttgactttgg
     4021 ttgcattttc actactgaca aagaattgca gtacaattac cacaaaaata aatgcaacga
     4081 atacaaaaaa tatcaggtta ttctttgaaa atattttatg catgacggtc actactttct
     4141 gatatcaatg gtgtattggt tttgattttt ggatttccta attgtaaacg ctgcttcgga
     4201 tcaagtaata acgttaataa atcagcaatc gtattgataa taacaacgaa gaagccaata
     4261 aataacacgc atccttgaat aacaggataa tctcgagatt taatactatc cattaataga
     4321 taaccaatac caggtatatc aaataaattt tcaatcacta cagtaccacc tattagactg
     4381 ccaagtgaaa tccctagtaa tgggataatc ggcaaaattg ttggttttag taaatcatga
     4441 attaaaatat aacgttcatt cataccgcgt aatcttgatg cttgtacgat attactttgc
     4501 aataacatca ataaattaga acgcactaaa cgaatgatgt atgcacacat acctaaagat
     4561 agcgtgatta caggtaatat aaactgactt agtataacgc tatctatatt cattaaattt
     4621 gtgacaataa ataataaaat aataccgata aagaacgctg gtaaactaat cgatagtgtt
     4681 gagatcactc taatcacttt atccgtccac ttatgaaatc gtttggctgc tataatgccg
     4741 agcggtatag atatgcataa cgacactact aatgttgaaa atgatatgag taatgttatg
     4801 ggtgcatagt tgaataatat ctgtgttacc ggttcttttg attcaaaact ttttcctaaa
     4861 ttaaaatgta ataaatgatt catccaatgc caccactgta ccaataaaga atcatttaat
     4921 cccaatttat ctttggttgc atttatttgt tccgtcgaca cttgtgctac atcaagatgt
     4981 aatattttat caacaggatt gcctggtgat aatttcatta aaatgaatgt aagtgtagaa
     5041 ataacaaata aaacaactat catttgcatc agtctataca acatagactt tattatgaac
     5101 ataatagtcc ccctccttgt gtaagttact aacactttct ttttacatga gaatggcgca
     5161 tgtatatgca acttacatat taagaactaa cgttcattat agtattatcc ataaagaaat
     5221 tgaagtatat ttaatttttt aacaaaatca ttataaaata taatattttg aatcaagtca
     5281 accatgtaaa atataaaaaa gtcaaaacaa aaacaactat agcactgtat tccatctctt
     5341 tcgaaataat tgttactgca gtgtaactta aaagtcgatg attttgtgca tatagttgtc
     5401 gaatattatt ttttatcttt acggcgaagt tcagcgccct catagccgta tttttcaatt
     5461 tgcttttcta atttacgcgc ttttctttct ttacgccaat ttctagtaaa ataccataat
     5521 agaaaactaa ttaataaact cataatcgct aaaaatgcag cgtatcctaa taatggttga
     5581 tattttatat cttgaaaatt tggaataaaa aatgcaagca cacctaatat aacaaatgta
     5641 attactgcag atacaaacca tttatttaaa actaagcaac agaatattgt taataaaatc
     5701 attattaatg ttgtgatcca t
//
LOCUS       DAKTGS010000158         5538 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.161, whole genome shotgun sequence.
ACCESSION   DAKTGS010000158 DAKTGS010000000
VERSION     DAKTGS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.161"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(370..645)
                     /locus_tag="O0286_002526"
     CDS             complement(370..645)
                     /locus_tag="O0286_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCY0170866.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            786..1268
                     /locus_tag="O0286_002527"
     CDS             786..1268
                     /locus_tag="O0286_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170867.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            1270..1437
                     /locus_tag="O0286_002528"
     CDS             1270..1437
                     /locus_tag="O0286_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCY0170868.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(1577..3424)
                     /gene="typA"
                     /locus_tag="O0286_002529"
     CDS             complement(1577..3424)
                     /gene="typA"
                     /locus_tag="O0286_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCY0170869.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            3526..3717
                     /locus_tag="O0286_002530"
     CDS             3526..3717
                     /locus_tag="O0286_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCY0170870.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(3871..4698)
                     /locus_tag="O0286_002531"
     CDS             complement(3871..4698)
                     /locus_tag="O0286_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0170871.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            4855..5469
                     /locus_tag="O0286_002532"
     CDS             4855..5469
                     /locus_tag="O0286_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCY0170872.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
BASE COUNT         1832 a          992 c          812 g         1902 t
ORIGIN      
        1 tcaataaact ttgaggtttt accaatatac cgtaaaatac ttctaaaatt cttcatatat
       61 tcagtcatcc aattctacaa tttatacttc aaatgatttc gttttatact ttaattttaa
      121 aaatccatgt tacttattat atacatgaca cagtcataat cacaaatatt aacaggtctc
      181 ttgaggtaat ttatagaata tttgtttact taatatcgtg aattgctaaa acccaataaa
      241 agtgactgac atgtcatcaa gaagacaaca taacagccac tttaatcagt aattaaacta
      301 taaatataaa atcggcgcag ctcaattgtc gatatctcac gccattttaa tatgcttata
      361 tattaaacct taaacaagtg taaaagcttc atgtaatttt gaaagttctt tctcaagacg
      421 taacataatt tgtttgccat cttcgcggtc aactaaacct aatttaacag caaaatcaac
      481 ttctttttga agtccaaaca tttgtgtatc tagtacttct tcatataatg ggcatgaagg
      541 taatgttaaa ttatctagtt gaactttaat cagatgcaag atttcatcag catctttagt
      601 caattgtttc aaagctgcat ttttcattgt tgcttgtttc gccatgacac gctccccctc
      661 tatataaatt ctcaagtctt actaaaagtt tatcttacta aatattaaaa agcaagtaga
      721 tttaaaatgt gtcgttagca atttgtgtta aaataatgct atatacattg atagggagtg
      781 ggaaaatgat acaaattaaa ggagccgtaa agtttcctat aacattagat agtacgactt
      841 ggattttcga tgatagaaaa gttagcatcg aagatttaga aaaaggtgta tttgatggtt
      901 caaaaccaat cgactttgaa gataatcatg aatggaatcg tgctatccta gaaggacaaa
      961 ctaatccgcc tactttaaat tcagaaataa aatataaaaa acgtgccatg ttagaaggtt
     1021 catttgtcat taatatgaca ccatttttca aacattccga accacatgac tctgcaacta
     1081 ctattcgatt atcaaatgag catgatcatt tagatatacc gatagatcta ttaccctatt
     1141 tatttttcca atttgcaaaa gacggtaaac gactttacag cgataatggt gtagatagtt
     1201 ttatttacaa tcaagaagat ggttattcat atcgaatcaa ccatgtaact catattgagg
     1261 tgatttaatt tgagacaagt gcaatgcatt atctgtgatg caaaagtatt tatagatgag
     1321 cgcacaactg agtctaaacg acttaaaaac aatcctattc gaacatttat gtgtgatgat
     1381 tgtaaaagtc gattagacac acctaaacaa cgtgcgcaac actatccgct cgattaatat
     1441 ctgaataaac aagttagaaa tttgaaaatg tgaaagtaac aggaaataat agcatttaaa
     1501 cactttttca aatattatat gccaaaagag aagctaagca actttcatcg ctcagcttct
     1561 cttttattat gttaaattat tcgttttctt gcatcatttg tttgatacgc tttgcttctt
     1621 tttcacgaac atttttgttt aaaattttct ttcttaaacg tatactttct ggtgtaacct
     1681 caacaagttc atcatcatta atgaattgta acgcttcttc caatgttaga atacgcggtc
     1741 tattcattgt ttgtgtttgg tctttcgttg cagaacgtac gttagtttga tgttttgttt
     1801 tagtgatgtt aacagttaaa tcattttcac gattatgttc accaacaatc ataccttcat
     1861 aaacttcagt accaggttcc atgaagttta cacctctatc ttcaagtccc aaaatggcat
     1921 aagtacttgc agaaccttga tccattgaaa ttaatgcacc attacgacga ccgccaattt
     1981 gtgctttaat acgtggtcta aattcttcaa atgtatggtt aataataccg taacctcttg
     2041 tcattgacat aaattcagtc gtataaccaa tcataccacg agccggtaca ttaaagatta
     2101 aacgtgtaag tccattatca gttgtagtca tatcaaccat ttcaccttta cgtgcaccta
     2161 atgattcaat aacagcacct gcattttctt gtggcacttc acattgcaca cgttcaaatg
     2221 gttcacacat tacaccatct atttctttaa taattacttg tggttttgaa acttgtaatt
     2281 cataaccttc acgacgcata ttttcaataa ggattgataa atgcaattca ccgcgaccag
     2341 caactaccca tgtatctgga gaatctgtgt tagaaacttt caaagataca tctgtttcta
     2401 attgttgatt taaacgttct tgaatttgac gtgctgttac aaagtcacct tcacggccag
     2461 caaatggaga attgttaact ttaaatgtca tttcaagagt aggctcatca atacgtagaa
     2521 ctggcaatgc ttcttgatgg tcatgtggtg ttacagtttc accaacatta atgtcttcca
     2581 tacctgaaac agcaattaaa tctccagctt gtgcttcttc aatttctaaa cgttttaatc
     2641 caaagtaacc aaagatttta gttacacgga agtttttcac tgtaccgtct aatttaatta
     2701 gtgatacatt atctccgaca cgcattttac ctctgaatac acgaccaata ccaatacgtc
     2761 caacataatc attgtagtcc aacaatgcta cttggaattg taatggctca tcactgttat
     2821 caattggagc tggtacataa tcaataattg tttcatataa tgattgtaaa ttatcatctt
     2881 gcttttcagg atctaagcta gctgtaccat ttactgctga agcataaaca acagggaatt
     2941 ctaattgttc atcgtttgct tctaattcaa taaataaatc taaaacttca tctacaacac
     3001 cctctggacg tgctgatggt ttatcaattt tattaacaac aacaacaggt ttcaggtttt
     3061 gttctagcgc ttttttaagt acaaaacgtg tttgaggcat tgtaccttca tacgcatcta
     3121 ctactaagac aaccccatca accattttca taatacgttc tacttctcca ccaaagtctg
     3181 catgtcctgg tgtatccaaa atattaatac gtgtaccttt ataatcaaca gccgtatttt
     3241 tggctagaat cgtaattcca cgctctcttt cgatatcgtt agagtccatt gcacgttcat
     3301 cgacatgttc attttctctg aatataccag attgttttaa caactcatct actaaagttg
     3361 ttttaccatg gtcaacgtga gcaataattg ctatattgcg gacatcttct cttttattag
     3421 tcatataaat agtttccttc tttcttaagt aaaatcccac ttttaattac aactttttta
     3481 ttatatcata taattgagaa aagataagca aaaggtgggg ataggatgaa acaaaaaaaa
     3541 tctaaaaata tcttttgggt attttctata ttagctgttg ttttcttagt attatttagt
     3601 tttgctgttg gtgcatcaaa tgtaccaatg atgattttaa catttatatt actcgttgca
     3661 acctttggaa ttggatttac tacaaagaaa aaatatcgag aaaacgattg gctataagtt
     3721 atatcgacaa taacacgcgt tgatttaaga ccacacatgt tcatgatgaa catttaactg
     3781 gttttatatt ggcgcgtgtt tttattattt aaggtataaa agcgtaatat gttacctaat
     3841 tgttaggact acatattacg cttttggtaa ttattttgat tttcttttaa atcgttgttc
     3901 atgtaattgt attaacgcat catggtgggg ctctaaataa tcattgctaa tttcttgatg
     3961 gagaccacga tttccaacta agattgaatt tggaccacta attgttaatg gttctcctag
     4021 taaattggaa gcttgtccat ttacttcata taaaataacc aatccgccag caaaatccca
     4081 tggttgaagt cttggcgtca tatatgcttc taaattacct gtagcaactg aaacgatttc
     4141 aagcgctgca ctaccatatg cccttgcact tctagaatca ttaacaattt ctttaaagat
     4201 ttctcctaaa attggtttag ttaaccagtt cggattgatc ccaataatgc tttgtcttag
     4261 attagaatca ttcaatggtt tcaagggttg gctaccacga tatgcacctt cccctacttt
     4321 agcatgatat aagacatcag ccataacatc atatacaaaa cctgcataag gtttaccatc
     4381 gatataaata ccaattgaaa ttgcgaaatt ttcttgttga tgaacaaaat tcaatgtacc
     4441 gtctattggg tcaacaatcc ataccgtacc tttggaagta tcgatgtcat gaccatgccc
     4501 ttcttcacct aatacttgat gattgggata tgtttctaaa attgtatcaa aaatgaaatc
     4561 ttctgttgct ttatctacat ttgtaacaag gtcattcgga tttgactttg tttcaattgt
     4621 taaattttgc tccatcaatt gtttaattct aattcctgct tcttgaataa gtccttgggc
     4681 aaatccataa agtgccataa catcatctcc taacatcttt attatacatc aacattttat
     4741 aaaaataact tcttatgata aaaatgaaaa tactaatttt aatgagagcg tcttaagccg
     4801 caattgataa aacatatgct acaatatttt taaacactat acaggaggta ctctatgaca
     4861 aaatatacat ttaaacctaa agatttcaaa gcgttcaacg tagaaggctt agacgcacga
     4921 atggaagctt taaacgaata catacgacca caactccgtg aattaggaga atattttagt
     4981 gacttcttta caagtcaaac tggtgaaaca ttttatcctc acgtagcaaa gcatgctaga
     5041 agaagtgtga atcctcctaa agatacatgg gttgcttttg caacaaacaa aagaggctat
     5101 aaaatgttac ctcatttcca aattggtatg tttgaagatc aactgtttgt tatgtttgga
     5161 atcatgcatg aagcaaaaga taaagcaaca cgtgcaaaag tttttgaaag aaaatttaaa
     5221 gctattcaac aattacctga tgattatcgt gtttgcttag atcatatgaa acctgataaa
     5281 ccatttatta aagatttaac ggatgatgat ttaatagaag cgatacaaag agccatcaat
     5341 gtgaaaaaag gtgaattctt tatagcgcgt gcaatcacac cacaagataa aagattaaaa
     5401 agtgacaaag catttattgc atttttagaa gaaaccttcg atcagttctt accattttat
     5461 tctgcataaa taactttgtt taaataatag agcacttaat cacatccatg atttcgtgct
     5521 cttttttctt aatattaa
//
LOCUS       DAKTGS010000159         5398 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.162, whole genome shotgun sequence.
ACCESSION   DAKTGS010000159 DAKTGS010000000
VERSION     DAKTGS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.162"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(204..389)
                     /locus_tag="O0286_002533"
     CDS             complement(204..389)
                     /locus_tag="O0286_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170873.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
     gene            complement(478..1542)
                     /locus_tag="O0286_002534"
     CDS             complement(478..1542)
                     /locus_tag="O0286_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCY0170874.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1690..3132)
                     /gene="lmrS"
                     /locus_tag="O0286_002535"
     CDS             complement(1690..3132)
                     /gene="lmrS"
                     /locus_tag="O0286_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCY0170875.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3454..3927)
                     /gene="sepA"
                     /locus_tag="O0286_002536"
     CDS             complement(3454..3927)
                     /gene="sepA"
                     /locus_tag="O0286_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCY0170876.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            complement(4026..5369)
                     /locus_tag="O0286_002537"
     CDS             complement(4026..5369)
                     /locus_tag="O0286_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170877.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT         1926 a         1003 c          693 g         1776 t
ORIGIN      
        1 caggatcaaa ctctccataa aaattatgat gtttgattag ctcataaata ctaaataatg
       61 tttgtaacta atagttacgt ttttggaatt aacgttgaca tattgtcatt cagttttcaa
      121 tgttcattaa tgttcaatct cttttattct acttcattta tttcttgaag tcaataactt
      181 tttgaacgat tactttattt attctatatt gtttttatag ttatttcaat ggtaagtttt
      241 acacttttga aattcttctt taaaaacaac tgcgtcgttt ttgacgactt tatcatatta
      301 tcaactttgg aatttaaagt caataacttt tttaaaaact ttttgtgttc acaacccgct
      361 tctttttcaa cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa
      421 atcaacataa aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta
      481 tttatttgta gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc
      541 atctgattga tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg
      601 taattcccca agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc
      661 aaagacatat tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac
      721 gccaagaata gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc
      781 tgctgtagga tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac
      841 atctaaagct acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc
      901 ccatttaact tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat
      961 aactggcgca ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc
     1021 aactggaatt acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg
     1081 tacactaaat ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa
     1141 ggcaacggct aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat
     1201 tgcaataaat tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt
     1261 ttcttctttt ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc
     1321 gaccgttttc gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg
     1381 cggtgcacca cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt
     1441 aatagaaact tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt
     1501 aatttctcct accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc
     1561 attttaagac atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta
     1621 taaaactttt tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact
     1681 ataattattt taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag
     1741 tgctatcaca taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc
     1801 tatagcaatg gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa
     1861 aaatattttt gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc
     1921 cattacacga ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga
     1981 attaattcct gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc
     2041 atatacgact attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt
     2101 agtaattgtt aaaattgtaa aaccggtata aatcaatggt ctaggaccat acttatcata
     2161 aaatttacct gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag
     2221 tcctgataac aaggcagata aagataaact gttttgaaca tatagcggta taagcagcgc
     2281 aggtccaacc attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct
     2341 atttttaaaa acccttaaat ttaaaagtgc attagtaata ttaaattgtc tacgtataaa
     2401 taaatagata ataaccatac ttaatatcaa cgcacctatt actattggac ttgtaaatcc
     2461 taaatctcct gcactactaa atgcgtatag cattaacccg aacccaatag ttgaatacat
     2521 tactgaacgc ttatctaatt tcgtatattt cacttcattg taactcgaga ttgaaaccaa
     2581 accgaaaaca aaggcaagta tagcaattcc taaaattata ataaatggaa ctcgccaact
     2641 cgcttgatca ataattaatc ctgtagctgt aggtcctatt gctggtgcaa attgaattac
     2701 taaacctgct agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt
     2761 aaattgcatt aagggcataa gtacgcctgc acccatcgct tgaattacac gagctaacat
     2821 taaaactcca aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat
     2881 cgccgctaag tataaaggtc tagttttaat tctatccatt agatatgccg tcagaggtat
     2941 catgacgcca ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattgat
     3001 atttaattct ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc
     3061 tacaaacgca ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc
     3121 aactttagcc atcataattc ctccttttta aaacagatat acacttgtcc ctttaatacg
     3181 tatagccatg aacataatgg gagtagtact caaataataa agaagcacta cataataaat
     3241 cattattggt tcttacacat taggcacagc taatgtgtac ttaaaaacta acaatataac
     3301 attgtagggc ctaataattt attttatgtc tcagactcat acaatggtta tttaacttat
     3361 ttaccacaaa ttgagcacta agactcacta ttatcgattc ttctattgaa caaaaagaag
     3421 ttggaacaca tatgtgccct caacttcaga tatctatttt ctattattta aatttttact
     3481 ttcagggtaa aattgaagct tacaaacata taagaaccct ataataacca tgatgaccca
     3541 aaaatcgata ttgatataca catagttcag atgaataata aaataaaata acgttgttgc
     3601 aactgtgtaa gaaatcaaat gaaaagtcgc gcctctaaaa tatgcatttt caggcaacat
     3661 ttttctaaaa taatccatta aatgctcgac tgcaaatatg atgaagtacc caaagattat
     3721 ataactgcca taatataata ggttatcgta catacttcta ttatatgtaa agtcacccaa
     3781 cctaaaataa ataagtattc tacttaatcc atataaacca aaccctagaa atgttaaaaa
     3841 aatcgcacca gaaagtacaa aaataaagag aacaatcatt gtagttaaca agttataaaa
     3901 cttatgttta agataattca caatcattgg cctccttatt aataaaatat tgaacgattt
     3961 atctagtatc taggaaattt attatttctt actttaatta cactacatca attttaatca
     4021 taattctatt cttttgattg agatgactgt tctttaaaaa atacgaccca taaaacaata
     4081 agcccaatgc taattacagc aacagcactt aacacattgt gtaagtttgg accaaagatt
     4141 cctgattgta tcgcataaag atatcccatt attgttgaac ctattgatgc cccaagattt
     4201 ttagttaatc cataaaatga catcattttc ttcatttgta atgggctagt ttcctcttgg
     4261 acaatcacac tatctttcgt ataaatatat ccaaaactta atcctgccaa aattaacaca
     4321 aatgctataa gtaccggcaa tttaatacca aatgaaatga taatactact tactagtagc
     4381 aatgtaaatg atagtaagta aataactttc cttgatagtt ttgcttcaat tcgatgtaaa
     4441 ttaaaattca atgtaatcca agctacagat aaaggaaaaa taaccaatcc actttgcaat
     4501 ggagataatc ctagttgttc ttgaaggtag actggaatat ataaattgaa tcccattaaa
     4561 caaatagctg ttagaaggtc agttataaaa actaaagtaa tcgaacgatt aaattcaacc
     4621 acaggtaaaa atggtgaaga aacatgtttt tctactttaa ataaacacat tgccacaacg
     4681 atagctaaga taaaactcaa gaaatttaaa agtaacagct gttggtttaa taaagcaaac
     4741 ataatcaatc caataaatac ataaaatagc gtgagccctt tagtatcaaa ttttgattta
     4801 gcaactgttt cttcctctgg aaaatgaaat gtccatataa ctaatataat ggcaatgatc
     4861 gcaatcggta tatttatgta gaataaccaa tgccatgttg caaattctaa tataccacca
     4921 cctagaagtg gcccaatgat actcgaaata ccccaaacac tgccaactat gcccataatt
     4981 ttatatcgta acggaatttc aaatgctaac tttggaacaa tttgcgataa tgacatcaat
     5041 acacctgatc caaaaccttg tatcacgcgt gatataatta gcatagtaaa attagtactt
     5101 aaaccacaca taaaactacc tatactaaat aaaagtaatc ctgcaattgc cacataaata
     5161 attttaaatc tcgaaagtaa ttcgcccact ataggattgg caattactaa tgcgataaaa
     5221 tacgctgtga aaattaatga aattaaattt cctgcattta aatcttgttt aattgtaggt
     5281 aatgcaagcg aaataataga tgattcaatc gctgacataa acataattaa tattaaagct
     5341 ataacagtta tgattgactt taatcgcatg aaatacctcc ttctatatat ttatttat
//
LOCUS       DAKTGS010000160         5337 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.164, whole genome shotgun sequence.
ACCESSION   DAKTGS010000160 DAKTGS010000000
VERSION     DAKTGS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.164"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            99..2444
                     /locus_tag="O0286_002538"
     CDS             99..2444
                     /locus_tag="O0286_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCY0170878.1"
                     /translation="MAWSDFMQKLAQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGY
                     LKEGKRRAGQVMNRSMLTLDIDYAAQDMTDILSMFYDFAYCLYSTHKHREISPRLRLV
                     IPLKRNVNADEYEAIGRKVADIVGMDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLP
                     LLDPDKILNEYVDWTDTLEWPTSSKEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEE
                     AISTFIPDLYEKHSTNRYTYHEGSTAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLV
                     RIHLYGAQDEDAKTDTPVNRLPSYKAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVN
                     SDDAWSETLEITSKGTFKASIPNIEIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTN
                     FKTRQWQDGDDSSLRSYIEKIYDIHHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGH
                     KRLEKLFIKYLGVEDTEVNRTTTKKALTAGIARVMEPGCKFDYMLTLYGPQGVGKSAL
                     LKKLGGAWFSDSLVSVTGKEAYEALQGVWLMEMAELAATRKAEVEAIKHFISKQVDRF
                     RVAYGHYIEDFPRQCIFIGTTNKVDFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEI
                     DQIWAEAKYYYEQGEELFLNPELEEEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDD
                     LTIFERRRFYQGDVDMLPTGNVDYVERNKVCALEVFVECFGKDKGDSRGSMEIRKISN
                     ILRQLDNWSVYDGNKSGKIRFGKDYGVQIAYVRDESLEDLI"
     gene            complement(2553..2651)
                     /locus_tag="O0286_002539"
     CDS             complement(2553..2651)
                     /locus_tag="O0286_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170879.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            2785..3075
                     /locus_tag="O0286_002540"
     CDS             2785..3075
                     /locus_tag="O0286_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCY0170880.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            3056..4423
                     /locus_tag="O0286_002541"
     CDS             3056..4423
                     /locus_tag="O0286_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0170881.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            4436..4873
                     /locus_tag="O0286_002542"
     CDS             4436..4873
                     /locus_tag="O0286_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0170882.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            5030..>5337
                     /locus_tag="O0286_002543"
     CDS             5030..>5337
                     /locus_tag="O0286_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0170883.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     "
BASE COUNT         2040 a          724 c         1064 g         1509 t
ORIGIN      
        1 tagacaaagt cactcaaata gaaacaatta aatatgatcg tgatgtctca tattcttatg
       61 ctgctagtcg tttatccaca tattggacta atcacaatat ggcttggtct gactttatgc
      121 agaagctagc acaaacagtt agaactaaag aagatttaac tgagtacaat aaaatgtcta
      181 agtctgaaca agcagatata aaagatgttg gcggatttgt cggtggatat ttaaaagaag
      241 gcaaacgacg tgctggtcaa gtcatgaatc gttcaatgct aacacttgat atcgattatg
      301 ctgctcaaga tatgactgac atattatcta tgttttatga ttttgcatat tgtttatatt
      361 caacacataa gcatagagag ataagtccaa gactgcgttt agtgattcct ttaaaacgaa
      421 atgtaaatgc agatgagtat gaagctattg ggcgtaaagt cgcagatatc gttggcatgg
      481 attacttcga tgatacaact tatcaaccac ataggttaat gtattggcct tcaactagca
      541 atgatgcaga atttttcttt acctatgaag atttaccttt gttagatcca gataaaatat
      601 taaatgaata tgttgattgg actgacacat tagaatggcc aacgtcttca aaggaagaga
      661 gtaagactaa aagattagca gataagcaag gtgacccaga agaaaagccg ggaattgttg
      721 gcgcattttg tagagcctat acgatagaag aagctatatc aacttttatt cctgacttat
      781 acgaaaaaca ttctactaac cgttatacct atcatgaagg ttcaactgca ggtggattgg
      841 tgttatacga aaataacaag tttgcctatt ctcatcataa tacggatccc gttagcggta
      901 tgcttgtgaa cagttttgat ttagtacgca tacacttata tggtgctcaa gatgaagacg
      961 ctaaaacaga tactccggtt aatcgactac ctagttataa agcaatgcag caaagagcgc
     1021 aaaatgatga agttgttaaa aagcaattaa ttaacgacaa aatgtctgat gcaatgcagg
     1081 atttcgatga aatagtaaat agcgatgatg catggtctga gacgttagaa attacttcga
     1141 aaggtacttt caaagctagt atcccaaata tagaaattat attgcgtaat gatccaaatt
     1201 taaaaggaaa aatagcattt aatgaattta caaaacaaat tgaatgctta gggaaagtgc
     1261 catggaatac taattttaag acacgtcaat ggcaagacgg tgatgatagc agtttaagaa
     1321 gttatatcga aaagatttat gacatacacc attcaggtaa aacaaaagat gccattataa
     1381 gcgtagcaat gcaaaatgct tatcatccag taagggatta tctaaataaa atatcgtggg
     1441 atggacataa acgtcttgaa aagttattta tcaaatactt aggtgttgaa gatactgaag
     1501 tgaatagaac aactaccaaa aaagcattga ctgctggaat tgctcgagta atggagcctg
     1561 gatgtaaatt tgactatatg cttacacttt atggtcctca aggtgtaggt aaatctgctt
     1621 tgctaaaaaa attaggtggt gcatggtttt ctgacagttt agtttctgtt acaggtaaag
     1681 aagcctatga ggccttacaa ggcgtttggc taatggaaat ggcagaactt gcagctacaa
     1741 gaaaagctga agttgaagct attaagcatt tcatatctaa acaagttgac cggtttcgtg
     1801 ttgcttatgg acattatatt gaagattttc caaggcaatg tattttcatt ggtacaacta
     1861 ataaagttga tttcttaaga gatgaaactg gtggaagacg tttttggcca atgactgtaa
     1921 atccagagag agttgaagtg aactggtcta aactaaccaa agaagagatc gaccaaattt
     1981 gggcagaagc taaatattat tatgaacaag gagaagagtt attcctcaac cctgaactag
     2041 aagaagaaat gcgttcaata caaagcaaac atactgagga atctccatat acaggcatta
     2101 ttgatgaata tcttaacaca ccaattccta gcaattggga tgacttaact atctttgaac
     2161 gaagacgatt ttatcaaggt gatgttgata tgttaccaac aggaaatgta gattacgttg
     2221 aaagaaataa ggtctgtgcg cttgaagtgt ttgttgaatg ttttggtaaa gataagggag
     2281 atagtagagg atctatggaa attagaaaga tttcaaacat cttaagacaa ttagacaatt
     2341 ggtctgtata tgatggtaat aaaagtggga aaattcgatt tggaaaagat tatggtgtac
     2401 agatagctta tgtaagagat gaaagtttag aagatttaat ataataaata ttgaataaat
     2461 atacatttta gagtgttgta tcagatgttg catcattttt tgagtgatgc aacacgtgag
     2521 tgtaaaaagt aatcgtaggt gttgcatcat ttttagtgat gcaacattga tgcaacaaat
     2581 gatacaacac ctctttccct tctcgctgta aggttcaacc ctgtttgttt ccaatgttgc
     2641 atcaaattca ctataaagtt taaaaagtag tgttagggag taaagaggta taggggtaac
     2701 cctctaacag ctatttttaa aagtttggca agaattgatg caacatcgga acacaaatat
     2761 aaattttgta tacaaggtga atatatgaaa gaatcgacat tagaaaaata tttagtgaaa
     2821 gagatatcaa agctaaacgg tttatgttta aaatgggttg cacctggaac aagaggtgtg
     2881 ccagatagaa ttattattat gccagaagga aaaacatatt ttgtagaaat gaagcaagaa
     2941 aaaggaaagt tgcatccttt acaaaaatat gtgcatagac aatttgaaaa tagagatcat
     3001 aaagtatatg tgttatggaa taaagaacaa gtaaatactt ttatcagaat ggtaggtgga
     3061 acatttggcg attgacttca aaccacatag ctatcaaaag tatgcaatag ataaagtgat
     3121 agataatgag aaatacggtc tgtttttaga tatgggtcta gggaaaacag tatcaacact
     3181 tacagcattt agtgaattgc agttgttaga cactaaaaaa atgttagtta tagcacctaa
     3241 acaagttgct aaagatacat gggttgatga agttgataag tggaaccatt taaatcatct
     3301 gaaagtgtct ttagttttag gaacacctaa agaaagaaat gatgcattaa acacagaggc
     3361 tgatatctat gtaaccaata aagaaaatac taaatggtta tgtgatcaat ataaaaaaga
     3421 atggccattt gacatggttg tgattgatga actgtctaca tttaaaagtc ctaagagtca
     3481 aaggtttaaa tctattaaaa agaaattacc actcattaat agatttatag gattaacagg
     3541 aacacctagt ccaaatagtt tacaggattt atgggctcaa gtttatttga tagacagagg
     3601 tgaaagactt gagtcttcat tcagtcgtta tcgagaaagg tactttaaac caactcatca
     3661 agttagcgaa catattttta agtgggagct aagagacgga tctgaagaaa agatatataa
     3721 acaaatagaa gatatatgtt taagcatgaa agcgaaagat tatctggata tgcctgacag
     3781 agttgatact aaacaaacag tagtcttatc agaaaaagaa agaaaagtat atgaagaatt
     3841 agaaaaaaac tatattttag aatcggaaga agaaggaaca gttgtagctc aaaatggggc
     3901 atcattaagt cagaaactac ttcaactatc taacggcgca gtttatacag atgaggaaga
     3961 tgtaagactt atacatgata agaagttaga taagttagag gaaattatag aggagtctca
     4021 aggccaacca atactattgt tttataactt caaacacgat aaagaaagaa tacttcaaag
     4081 gtttaaggaa gcaaccacat tagacgattc aaactataaa gaacgttgga acagtggaga
     4141 cattaagttg cttatagcac atccagcaag tgcaggacat ggattaaact tacaacaagg
     4201 tgggcacatt attgtttggt ttggacttac atggtccttg gaattatacc aacaagcaaa
     4261 tgctagatta tacagacaag gacaaaatca tacgactatt attcatcata tcatgaccga
     4321 taacacaata gatcaaagag tatataaagc tttacaaaat aaagaactaa cgcaagaaga
     4381 attaatgaaa gctattaaag caagaatagc taagcataag taatggaggt ctaacatggg
     4441 gaacacaata tatgatatca agccaggcac atttaaatat attgaatcag aaatatataa
     4501 tttaaatgag aacaagaaag aaataaaaag attgaggttg gaaatactta atccaacgaa
     4561 ggaacaagat tccaatattg tatatggacc attacaaaaa ggcgaaccag ttagaacaac
     4621 tgaactaatg gcaactagat tattaactaa taagatgtta cgaaaccttg aagaaatggt
     4681 cgaagcagtt gagagtgaat acttaaagtt gcctgaagat cataagaaag taataaggct
     4741 caagtattgg aataaagaaa agaagttaaa gatggaacag ataggacatg aatgccatat
     4801 gcatcgtaat actgttacta ctatacgaaa gaactttgtt aaagcggtag cgtatcatgc
     4861 aggtatcaaa taacattgtg caaagattgt gcaaaaggcc tacaaatctg tagtaatatg
     4921 atagtatcgg aaagatgtat aaagttatct aaaagttata cgacacaagt acacgaggca
     4981 catcgctatg cggtgtgtct tttgttatgc aatcaaagag gtgtaagaga tgaccaagca
     5041 caataacatt tataagcatg gtcgtaagtc atatcaatac gattggttct atcattcaaa
     5101 agcatggaag aagttaaggg agatagcatt agatagagat aatcatcttt gtcaaatgtg
     5161 tttacgcgaa gatattgtaa cagatgcaaa catagtgcat cacattattt atgttgatga
     5221 agattttaac aaagctttag aattagataa tctaatgtca gtttgttatg gctgtcataa
     5281 caaaattcat gcaaatggta atgacaaaag taatcttaag aaaattagag ttctaaa
//
LOCUS       DAKTGS010000161         5219 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.165, whole genome shotgun sequence.
ACCESSION   DAKTGS010000161 DAKTGS010000000
VERSION     DAKTGS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.165"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..560
                     /locus_tag="O0286_002544"
     CDS             <1..560
                     /locus_tag="O0286_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0170884.1"
                     /translation="IQNTNNIANPNLIFIGQKLKVPMTPLVEPKPKTVSSNNKSNSNS
                     STLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGAKDIPYANNFNSEA
                     KIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKFQSLDQNWNNGGWR
                     KAEVAHKVVHNYENDMIFIRPFKKA"
     gene            588..1832
                     /gene="fmhC"
                     /locus_tag="O0286_002545"
     CDS             588..1832
                     /gene="fmhC"
                     /locus_tag="O0286_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCY0170885.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSK
                     KNKSKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPK
                     YNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEEL
                     IGDFIKPVRPILYKFAKLIYKV"
     gene            2005..2877
                     /gene="dprA"
                     /locus_tag="O0286_002546"
     CDS             2005..2877
                     /gene="dprA"
                     /locus_tag="O0286_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCY0170886.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            3051..5126
                     /gene="topA"
                     /locus_tag="O0286_002547"
     CDS             3051..5126
                     /gene="topA"
                     /locus_tag="O0286_002547"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCY0170887.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
BASE COUNT         2065 a          673 c          912 g         1569 t
ORIGIN      
        1 atattcaaaa tacaaataat atagcaaatc ctaatttaat atttattggt caaaaattaa
       61 aagtgccaat gacaccatta gtagaaccaa aaccaaaaac agtgtcttca aataataaaa
      121 gtaatagtaa tagcagtaca ttaaattatt tgaaaacatt agagaataga ggatgggatt
      181 tcgacggtag ttatggatgg caatgtttcg atttagttaa tgtatattgg aatcatcttt
      241 atggtcatgg attaaaagga tatggagcta aagatatacc atatgcaaat aattttaata
      301 gtgaagctaa aatttatcac aacacaccaa ctttcaaagc tgaacctggg gacttagtgg
      361 tttttagtgg aagatttggt ggaggatatg gtcatacagc tattgtctta aatggtgatt
      421 atgatggaaa attaatgaag ttccaaagtt tagatcaaaa ctggaataat ggtggatggc
      481 gtaaagcaga ggttgcacat aaagttgttc ataattatga aaatgatatg atttttatta
      541 gaccatttaa aaaagcataa tttaaatcga aggcaggaca ttgaaatatg aaattttcaa
      601 ctttaagtga agaagaattt accaactaca ccaaaaagca cttcaaacat tatacacagt
      661 ctatagaatt atataattat agaaataaaa taaatcatga agcacatatt gtgggagtga
      721 agaatgataa aaatgaagtt atagctgcat gtttattaac agaggcacga atttttaaat
      781 tctacaaata tttctactct catagaggtc ctttacttga ttatttcgat gctaaattag
      841 tttgttactt ttttaaagaa ttatctaaat tcatttataa aaatagagga gtatttattc
      901 ttgttgatcc atatttaata gagaatttaa gagatgcaaa tggtaggata ataaagaatt
      961 ataataattc agtgatagta aagatgctag ggaaaattgg gtatctccat caaggttata
     1021 caacaggata ttcaaataaa agtcaaatta ggtggatttc tgtattggat ttaaaagata
     1081 aagatgagaa tcaactttta aaagaaatgg aataccaaac tagaagaaat ataaaaaaga
     1141 ctattgagat tggtgttaag gttgaagatt tatctattga agaaacaaat cgattttata
     1201 aattgtttca aatggctgaa gaaaaacatg gttttcattt catgaatgaa gattatttta
     1261 aacgaatgca agaaatatat aaagataagg caatgttaaa gatagcttgt ataaatctta
     1321 atgaatatca agataaatta aaaatacaat tattgaaaat cgaaaatgaa atgatgactg
     1381 tgaacagagc attaaatgaa aatccaaatt ctaaaaaaaa taaatcaaaa ttaaatcagt
     1441 taaatatgca attatctagt attaataata gaattagtaa aaccgaagaa ctaatatttg
     1501 aagatggacc tgttttggat ttagctgctg ctttatttat atgtactgat gatgaagttt
     1561 attatctatc aagtggatca aatccgaaat ataatcagta tatgggtgca tatcatctac
     1621 aatggcatat gataaaatat gcaaaatcac ataatattaa taggtataat ttttatggaa
     1681 taacaggcgt ctttagtaat gaggcggatg attttggtgt tcaacaattt aaaaagggtt
     1741 ttaatgcaca tgttgaagaa ttaattggtg atttcatcaa accagtaaga ccaattctat
     1801 ataaatttgc aaaacttatt tataaggttt aattataaag tatgttggaa attgaaattt
     1861 taaattcttt ccaacatact tttcactttt taagtcagta tttttactcc ttaaaataac
     1921 attacatcga caatgttttt gtattagaaa tgtaaaacaa aaatagaata tgattttgta
     1981 tgattacaaa aaggagttga tttattgatt aaactatttt tgcttaagtt atactgggca
     2041 cacttttcga ctaaacaaat tcatcaattt ttaatggcat atcctaatgt aattaaagag
     2101 gagggaagaa aaaaagatag ttatttatgt gaatgggtga atagggaaga aaatgttcat
     2161 ttattacgta aatactatgc ttttataaaa cttgatcata acgatattat taaagaactg
     2221 cagaaattaa aagtaagtta cattacatat atggattctg aatacccagt gctattaaaa
     2281 gaaatatatc aatttccatt acttcttttc tataaaggga acatcaaatt aataaataat
     2341 atgcatcatt tggcagtagt aggtgcaaga gattctacaa gttataccca acagtcttta
     2401 gaatttttat tatcaaatga taaaagcaaa tatttaacaa ttgtttccgg ccttgctcaa
     2461 ggagctgatg caatggcaca tcaaatagct ttaaaataca atctccctac aattgcagtt
     2521 ttagcctttg gccatcaaac acattatccc aaaagtacat tagcattaag aaataaaata
     2581 gaagaaaaag gtttagttat atctgaatat ccaccacata caccaatcgc taaatataga
     2641 tttcctgagc gcaatagaat tatcagcggt ttgtcaaaag gggttttaat tactgaggct
     2701 aaggaacaaa gtggcagtca catcacgata gattttgcat tagagcaaaa tagaaatgtt
     2761 tatgttttac ctggatctat gtttaatcct atgacaaaag gtaatttatt acgtatccaa
     2821 gaaggtgcta aggtagtatt aaacgctaat gatatatttg aagactacta tatttaaaac
     2881 taaaaataac gagttgcttc atatttcttt tgtttccctt tgtaatttat tttttaataa
     2941 aatctataaa aaatagacag ggaaaatgat ttgtttagat ataaaacgtt gacaaaagca
     3001 aaattaagcg tttatcattt atctttagta attagattag cgagggggaa atgacattgg
     3061 cagataattt agtcattgtt gaatcgcctg caaaagcaaa aaccattgaa aagtatttag
     3121 gtaagaaata taaagttata gcttcaatgg gacacgtcag agacttacca agaagtcaaa
     3181 tgggtgtcga cactgaagat aattacgaac caaaatatat aacaatacgc ggaaaaggtc
     3241 ctgttgtaaa agaattgaaa aaacatgcaa aaaaagcgaa aaacgtcttt ctcgcaagtg
     3301 accccgaccg tgaaggtgaa gcaattgctt ggcatttatc aaaaatttta gagcttgaag
     3361 attctaaaga aaatcgcgtt gttttcaacg aaataactaa agacgctgtt aaagaaagtt
     3421 ttaaaaatcc tagagaaatt gaaatgaact tagtcgatgc acaacaagcg cgtcgaatat
     3481 tagatagatt agttggctat aacatctcgc cagttctttg gaaaaaagta aaaaaagggt
     3541 tgtcagcggg tcgagttcaa tctgttgcac ttcgtttagt cattgaccgt gaaaatgaaa
     3601 ttcgaaactt taaaccagaa gaatattgga ctattgaagg agaatttaga tacaaaaaat
     3661 caaaattcaa tgctaaattc cttcattata aaaataaacc ttttaaacta aaaacgaaaa
     3721 aagatgttga gaaaattaca gctgcattag atggagatca attcgaaatt acaaacgtga
     3781 ctaaaaaaga aaaaacgcgt aatccagcaa acccatttac aacttctaca ttacaacaag
     3841 aggcggcacg taaattaaac tttaaagcaa gaaaaacaat gatggtcgca caacaattat
     3901 atgaaggtat agatttgaaa aaacaaggta cgattggttt aataacatat atgagaaccg
     3961 attctacacg tatttcagat actgccaaag ttgaagcaaa acagtatata actgataaat
     4021 acggtgaatc ttacacttct aaacgtaaag catcagggaa acaaggtgac caagatgccc
     4081 atgaggctat tagaccttca agtactatgc gtacgccaga tgatatgaag tcatttttga
     4141 cgaaagacca ataccgatta tacaaattaa tttgggaacg atttgttgct agtcaaatgg
     4201 ctccagcaat acttgataca gtctcattag acataacaca aggtgacatt aaatttagag
     4261 cgaatggtca aacaatcaag tttaaaggat ttatgacact ttatgtagaa actaaagatg
     4321 atagtgatag cgaaaaggaa aataaactgc ctaaattaga gcaaggtgat aaagtcacag
     4381 caactcaaat tgaaccagct caacactata cacaaccacc tccaagatat actgaggcga
     4441 gattagtaaa aacactagaa gaattgaaaa ttgggcgacc atcaacttat gcaccgacaa
     4501 tagatacgat tcaaaagcgt aactatgtca aattagaaag taagcgtttt gttcctactg
     4561 agttgggaga aatagttcat gaacaagtga aagaatactt cccagagatt attgatgtgg
     4621 aattcacagt gaatatggaa acgttacttg ataagattgc agaaggcgac attacatgga
     4681 ggaaagtaat cgacggtttc tttagtagct ttaaacaaga tgttgaacgt gctgaagaag
     4741 agatggaaaa gattgaaatc aaagatgagc cagccggtga agactgtgaa atttgtggtt
     4801 ctcctatggt tataaaaatg ggacgctatg gtaagttcat ggcttgctca aacttcccgg
     4861 attgtcgtaa tacaaaagcg atagttaagt ctattggtgt taaatgtcca aaatgtaatg
     4921 atggtgacgt cgtagaaaga aaatctaaaa agaatcgtgt cttttatgga tgttcgaaat
     4981 atcctgaatg cgactttatc tcttgggata agccgattgg aagagattgt ccaaaatgta
     5041 accaatatct tgttgaaaat aaaaaaggca agacaacaca agtaatatgt tcaaattgcg
     5101 attataaaga ggcagcgcag aaataatatt tttatttcct agatacattt taagattgtt
     5161 aaatagaatc attagtgaat cttattttaa agatagtaaa ggattaatct aaataagtg
//
LOCUS       DAKTGS010000162         5058 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.166, whole genome shotgun sequence.
ACCESSION   DAKTGS010000162 DAKTGS010000000
VERSION     DAKTGS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.166"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            52..836
                     /locus_tag="O0286_002548"
                     /pseudo
     CDS             52..836
                     /locus_tag="O0286_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            833..2157
                     /locus_tag="O0286_002549"
                     /pseudo
     CDS             833..2157
                     /locus_tag="O0286_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2157..2483
                     /locus_tag="O0286_002550"
     CDS             2157..2483
                     /locus_tag="O0286_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170888.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            2486..2773
                     /locus_tag="O0286_002551"
     CDS             2486..2773
                     /locus_tag="O0286_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170889.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            3090..3197
                     /locus_tag="O0286_002552"
                     /pseudo
     CDS             3090..3197
                     /locus_tag="O0286_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
     gene            3309..4103
                     /locus_tag="O0286_002553"
     CDS             3309..4103
                     /locus_tag="O0286_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0170890.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(4107..4229)
                     /locus_tag="O0286_002554"
     CDS             complement(4107..4229)
                     /locus_tag="O0286_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170891.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            4664..4855
                     /locus_tag="O0286_002555"
     CDS             4664..4855
                     /locus_tag="O0286_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170892.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
BASE COUNT         2021 a          665 c          908 g         1464 t
ORIGIN      
        1 ttgtgtaaat taaagattga gtcagtatta aaaatataag gtggagaaat gatgattcat
       61 tcaaagaaat taacgctcgg tatatgtttg gtattactca ttatattgat tggaggttgt
      121 gtcattatga caaaaacaaa tggtcgaaac gctcaaatta aagaaaattt caacaaaaca
      181 ttaagtgtat atccaaccaa gaatctcgat gatttttacg ataaggaagg ttttcgagat
      241 caagaatttg ataaaagaga taaagggact tggattattt attctgaaat ggttatcgaa
      301 ccaaaaggga aaaatatgga atcgagagga atggtgctct atatcaatcg caatactaga
      361 acaacgaagg gtaattttat tgtcaccgaa ataactgaag atagtaaagg atattcacgt
      421 agtaaagaaa aaaatatcct gtcaagatgg aaaataatcg aattattcca acaaagccta
      481 taccggatga caagttaaaa aaagagattg aaaactttaa gttctttgta caatatggaa
      541 attttaaaga ttttaaagat tataaaaatg gtgatatttc atataatcct aatgtgccaa
      601 gttattctgc aaagtatcaa ttgaataatg atgactataa tgttcaacag ttaagaaaac
      661 gatatcatat tccaaccaaa caagcgcccg aattaaaatt gaaaggatcc ggcaatttaa
      721 aaggctcatc cgtaggatct aaggatctag aatttacgtt tgtagaaaat caagaagaga
      781 atatctattt ttcagattcg gtcgaattta cacctagcga ggatgataaa tcatgagtca
      841 aacggaatat caaataaaat ctggcaatat aaaaggtaac tctgaagaaa caagtacagt
      901 atctaatata agttatgaaa tagaaaacgc aaataacagt ggtttaaaac aaaataaaat
      961 tgataaacaa attaaaaagt tacaagaaaa aaataaattc cctaaaaatc tttcatatct
     1021 taaaagttat acggacccca aaacaggcac gactacaagc gcctttttaa ataaagacac
     1081 tggcaaagtt actttaggta tgacaggtac taatgtacac aaagacgcaa tattaaaaca
     1141 aacatttggt gttccttctt atcaaggata tatagatgtg agtgaaacgc taaaggatat
     1201 tggggccgat gtcaatattg gccttcattc cgtcacagat aaagatccac attataaaaa
     1261 tacccaagac tttatcaaaa atatcaaaaa agactatgat attgatatta ttaccggaca
     1321 ttcgctgggc ggtagagatg cgatgatttt aggtatgagt aatgatatta aacatatcgt
     1381 tgtgtataat ccagctccat tagcgatcaa agatgtgagc ggcctttatg ccgatcaaga
     1441 agaacttaaa aaattgattg aaaagtacga tggtcacatt gtaagatttg tgtctgatga
     1501 agacgaatta gatgcaggtg tccgcaatca tttatatgaa actgctggag aaaaaatagt
     1561 acttaaaaat ggagaaggcc atgcaatgag tggtatttta atgagcagaa cacaggctat
     1621 aatcttagct gaattaaaca aagttaaagg ctaccaagac gaaaataata aagcattaaa
     1681 atccgttcgt aaacaaacga ggcatagatt acataaagta gagacgttaa gagcgaattg
     1741 gattcaaaca acgggtggat cactctcttc ctcccaacaa caattattag aagctttaac
     1801 agcactaacc attgccgaag gcttaaatca attagtgaat gaagaaagcc aacatttgaa
     1861 aaaatgtatc acgcgatggc acataaattt ggagacaact ggaaaaaagc gcaagaagtt
     1921 ggaaatgaaa ttggtgaaaa attaacctct gaagaggtta tagatgaatt aagaaaaggt
     1981 ggcgcgtatg aaagtaaact tgaaacagat cccaaaagaa aaattgatga taagataaag
     2041 aaattaaatg atgtttataa aaattgtaat ggctatatcg caaaaattaa acagagtatc
     2101 gaagcaattg tttctaatga ccaaatgtta gcgagccaga ttgatgggat gatgtaatgt
     2161 ttactacgta taaaaatatt aatgaacttg aaaatgccta tgatgaagaa agaaaacaat
     2221 tgaatgatgc attcaatcaa attgatgaat taagacatca aacacgcaag aaatgtgaac
     2281 aaatgtatga tcatttctta tatctcaaac ataaaatgaa ttatagtgaa gacgctatga
     2341 tcaggatgac acgtattata gaatctttcg atagagaaac gaatcaacgt atccgacatc
     2401 acgaaatgaa attagaagat tataaagatg agttaagaag agaatatcta aaacaatctg
     2461 acagaattga aggagatgaa taagcgtggg acttgatttt agtggtttac cagatttagc
     2521 agtattggaa caaatgaagg aaaaagaaca gattagtgag gttattgcgc ctgaacatgt
     2581 tcgtatgcat catgatcatc aaaataagct gaaaagtgat gagaaaatat tacttgacca
     2641 aatggtaagt catttcaaaa aatttgaaga tgattttaaa aatgcggcac aaggggcttg
     2701 ggtgaaaaat gccacagacg aattaaaaga tattagtaat gatttagaaa aaattcaaga
     2761 tattaaagta taaaaaggta ttaagaaaca caataagtat ataatccatt taataataaa
     2821 tgaattatat agttcataat ttcgactata agtggctatt agccattatt tttcgggatc
     2881 tatgtcaaat cggactaatg aattcaataa tggaagttaa gcaaccaatc tttgtttaac
     2941 ttctttttta ttttttggaa aataaagttt tgaacataat aaaatttgat tatgttttaa
     3001 cgaattttga tgtttcttga actatatatc atctagtcgt catttacaat tggtaaatat
     3061 gacttcaaac tgtatgaagg tgatggcgat taaaaggctc atccgtaggt tctaaagaac
     3121 tagaatttaa ctttataaga aataaagaag aaaatgtgta tttttctgat agtattaatt
     3181 tcaaacctac tgaatagggt taatagaagt ttgaaataaa aatttcataa agtgttcaaa
     3241 ataatttttc gctttagttg taaatattaa aaactgagtc aatattaaaa atataaggtg
     3301 gagaaatgat gattcattca aagaaattaa cgctcggtat atgcttggta ttactcatta
     3361 tattgattgt aggttatgtc attatgacaa aaacaaatgg tcgaaacgcc caaattaaag
     3421 acacatttaa tcaaacatta aaattatatc caaccaaaaa tctcgatgat ttttacgata
     3481 aagaaggctt tcgagatcaa gaatttaaaa agggtgataa aggtacttgg atagttaatt
     3541 ctgaaatggt aatcgagcca aaaggtaagg atatggaaac gagaggaatg gtgctctata
     3601 tcaatcgcaa tactagaacc acaaaagggt attattttat aagtgaaatg acagatgaca
     3661 gtaacggcag accaaaggat gatgaaaaaa ggtatccggt aaaaatggaa cataataaaa
     3721 tcataccaac gaagccacta ccgaatgaca agttaaaaaa agagattgaa aactttaagt
     3781 tctttgtaca atatggcaac tttaaagata ttaatgatta taaagatggt gatatttcat
     3841 ataatcctaa tgtaccaagt tattcggcaa aatatcaatt gaataatgat gattataatg
     3901 tccaacagtt aagaaaaaga tatgatattc caaccaaaca agcgccgaaa ctattattga
     3961 aaggcgatgg agatttaaaa ggttcatccg taggttctag aagtcttgaa tttacctttg
     4021 tcgaaaataa agaagaaaac atttatttta cagattctgt acaatatact ccaagtgagg
     4081 atacaaggta tgagtcaaac tgaatatcaa ataaaacctg gtaatataac aagtaactct
     4141 gaagaaacaa gttcgatatc taaagtgagc tgtgaaatat aggtagccat ttcaaaaaat
     4201 ttaaaggtga atttgataat gtagctcaag gagattgggt taaaaaggcg aagaatgaac
     4261 tggatgatat tagtaagaaa ttaaaaaata ttcaaagaac ggaagtttaa tagcttatat
     4321 gattcttgga gctaagacag catgcgttca ttcatgccat tattaatata agcaccgcaa
     4381 caaaaaagct tctaatgtga tacaggaacc tcatattccg tatcatgtta gaagctttta
     4441 atgtctaaag aacatctaca ttttatcata ttttctgact tattaaactt ttatataatt
     4501 aaatatttct taattttcca aaatagtgat aaatttgtga aatacatcac aaatcccttt
     4561 atttatttgg aaattcatgt aatattagac ttgtaagaag ttaataaata gagagagacg
     4621 agagagttta tataaatact atataaacat tggagtgatg attatgagaa aagagattga
     4681 agcgcttatt ttctcagacg taagtagcta tgatatttac gtgaacactg gtgtaaatca
     4741 aggattaatt ggtgacatca aagatggtta cctaactatt gattctatgc cttacattga
     4801 tgctgagcgt ttgtatcact ttgctatgga acgtaaatcg ttagtcacta actagttctt
     4861 attgccaatg attactaccc ctagtcggcg gcaattgaag tgtgattgat gtaacttgcc
     4921 ctcgttggtg agcaattgag ggcagacccc tttaattaag taaaccctaa ctccccacaa
     4981 atctggaacg atactaaaag ccacgtccta tattggatgt ggctttagtc atacttatat
     5041 tatttttaaa acgattac
//
LOCUS       DAKTGS010000163         5037 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.167, whole genome shotgun sequence.
ACCESSION   DAKTGS010000163 DAKTGS010000000
VERSION     DAKTGS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.167"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(267..1013)
                     /locus_tag="O0286_002556"
     CDS             complement(267..1013)
                     /locus_tag="O0286_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCY0170893.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(1018..1491)
                     /gene="ytkD"
                     /locus_tag="O0286_002557"
     CDS             complement(1018..1491)
                     /gene="ytkD"
                     /locus_tag="O0286_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCY0170894.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1557..1814
                     /gene="yidD"
                     /locus_tag="O0286_002558"
     CDS             1557..1814
                     /gene="yidD"
                     /locus_tag="O0286_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCY0170895.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1811..2812)
                     /gene="menC"
                     /locus_tag="O0286_002559"
     CDS             complement(1811..2812)
                     /gene="menC"
                     /locus_tag="O0286_002559"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCY0170896.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2817..4295)
                     /gene="menE"
                     /locus_tag="O0286_002560"
     CDS             complement(2817..4295)
                     /gene="menE"
                     /locus_tag="O0286_002560"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCY0170897.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(4454..4909)
                     /locus_tag="O0286_002561"
     CDS             complement(4454..4909)
                     /locus_tag="O0286_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCY0170898.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         1505 a         1017 c          768 g         1747 t
ORIGIN      
        1 gcatagcgta tggttttctt tttacaatta aagagccaac cgttgttata gtctaacaat
       61 ggttggctcc tcttatttta tgtgctaaaa atttataggc aattttatta caacaatgta
      121 catttaaggt gaccttcatg ccaaaatcgc atcactcatt taatggaagc agcacgtctt
      181 catataaagt accgatccct aattcaacgc atgtagtacc acatcttcaa agcttgatag
      241 ttcccatgcg cacaccacgt ttcatactag ctatgcgact caacttggtt cataaactct
      301 ttaatataag tcaatgtttc aaccatcgct ggtggtcttg gcacatgtcc ttctgccatt
      361 tgataaaatg tttcatgcgt ggcacctttt aactctagtt ggtccgctaa ataatacgca
      421 tgatgaatac caacttgctg gtctttccct ccatgtacaa ttaatattgg cggactgttt
      481 tcattaatgt ttggaatcgc ttggcgtgcc tcatatgccg ctcgatcttt tttcggatga
      541 ccaatcattc ttcgtagcat gcctcttaaa tcgacacgtt cttcatacat taaatcaata
      601 tctgagacac caccccagat tgtataactt gttactggta agtcttgaaa tgtcaacaat
      661 ccttgtaaac cacctcgcga aaaaccaacc atgtggataa atgcatgtgg atatttatca
      721 tgtagcaacc ttaataattg cgtcacatca tttaaatcgc cacggtaaaa ttcgtctttg
      781 ccttcactcc cattgttacc tcggtagtat ggcccaatca ctaaagtttg actatctgaa
      841 aattgcatta atctacctgc gcgcacacgt cctacttgac ctttgccacc tcgcaaataa
      901 actacaatgc gatttacttc atgatgtggt gtcatcatta aagcttttac ttgtaagtca
      961 tctgacaaat atgtaatttc ttcgaattga tgcgtaaaat attcaattgg cattcgttta
     1021 cgtttgataa aacccaagtg attgcaccct ctctacgcat tttaaaatgg tactatcttg
     1081 cagtaagaaa ctccgttgtg cgagttcaat atcattgata cagttaaaca acactggccc
     1141 tgctgtttct aaataatcgt tcttgcttac caatgattca acttcgataa aatatacatc
     1201 ttttacaaaa tcagtttgat catgtgtttc aatggtatat tgtgctatgt aataaatatt
     1261 tttaactttg gcgcctgttt cttcatataa ttcacgtgta actgcttcag cactactttc
     1321 cccgcgttcc cttttaccac caggaaattc aatcccccgt aaattatgtt tggtaaaaag
     1381 caattgattt ttaaacgttg gaatagctag cacatgattg ccatctgcta tctcattatc
     1441 ctttttaaat gtcaaattaa cttgacgatt atctttatcc ctaaacttca cgcgcatcac
     1501 atccctacat tgtatgttaa tataatagtt aattactatc gttggaggca ttaattatga
     1561 aaaagatatt cttggcgatg attcattttt atcaacgttt catttcgcca ctcactccac
     1621 caacttgtcg tttttatcca acatgttcag agtacactag agaagcgatt caataccacg
     1681 gtgctttcaa aggcctttat ttaggtatcc gtcgtatttt aaaatgtcat ccgcttcata
     1741 aaggcggctt tgaccctgtt ccgttaaaaa aagacaagtc agcaagcaag cattcacata
     1801 aacataacca ttaatatggt tgtaattgag ttatatccac taaagggggg cgaaattcga
     1861 gtcgcccctc ttttaatatg cctgaatgcg ccaccacatc ttgttcaaaa taataacctg
     1921 ctggtgtaac atctcctgga taatcacctt tacgagcaag catcgctgta aaatagcggc
     1981 ttaaaccata ttcgtacatg ccgccaataa ccacttttgc accatgactt ttcaaagtat
     2041 caattgccgt ttgcacttta tcaatgccac ctagacgaaa tggttttaat acaacaactt
     2101 tcacattgta taattctatc aaattaatta tgtccaacaa cgatgttgcc ttttcatcaa
     2161 gggctattgg aggtattgtt ccatccgcta cttcatcaag catggagata tctttaaatg
     2221 gctcttcgat ataaagaacc tgttcacgcg ctaataactg taactgtgtg aaatcttgac
     2281 gatccaagga ctcatttgca tctataacca attgaaagtg aaagtctaat tcccgtaaca
     2341 ctctaatttg atgcatgatt tgaggcgtcc attttaattt aattctggtc ggctttgttg
     2401 cttttaatga ctctagttgt ttatttgata agccgctcgc tgtcgctcca tatgctactg
     2461 aaaatgaagg cagtacatga aacatttgat acaatgccat gacaatagtt gcccttgcag
     2521 caggcgtatt ttccaatgaa tctactaatt ttagtgctgc ttcatacgtt tcaaatgatt
     2581 tatttctatt atcttcgaac cattgctcaa ttacatgttt cactgaggca attgtttcat
     2641 gatcatacca atctgtttga aaagcgttac attccccgaa atatgcattt cctttgtcat
     2701 caatcaattc gataaacaaa caatcacgat gcgttaaagt gactttcggt gttacaattt
     2761 gtgacttaaa tggctcacta tatttataaa aatgcaaagc tgtcaacttc atcaaatcat
     2821 cctctataca acttatttct ttgtaattta cctgttgatg tataaggtaa agtatcaacc
     2881 ttttcaaagt gtttcggtac tttatatttc gctaaatgtt gtgataaata tgcaatcaat
     2941 tgtgcctttg aaatgtcact ttcactgaca aaatataatt taggcacttg gccccaagta
     3001 tcatcaggat gccctacaca tactgcgtca ctgatacctg gaaattgctt cgctaccgtt
     3061 tcaatttgat atggataaat attttcaccg ccactaataa ttaaatcttt acgtcggtca
     3121 taaatcatga cataaccttc atgatctatt tcagcaatgt cacccgtatt aaaataacca
     3181 ttttcaaacg tacccgttaa atctgttgga tacaaatata cattcatcac attggcgcct
     3241 ttaatcatta attctccatg accttcttta ttaggatttt taatttttac gtcaacattg
     3301 gcacttggca tccctacagt gtcaggacgt gcatgcaaca tttccggtgt tgctgttaaa
     3361 aattgcgaac atgtctcagt cataccaaat gaattataaa ttggcaggtt atattgtaat
     3421 gccgtctcta tcatagtggc agataattta gcaccgccga gtaatatttt ttgtaaatta
     3481 taaggttcat gtaaaccttg ttgcataagc caatttaaag tttgtggcac aagcgaaatg
     3541 tgcgtgattc gttcattttt aatcatcgtt aaaatttgtt cggcattgaa tttatcaaca
     3601 atgcgcacag taaaaccttc aataacagct cttaaaagta cactgagacc cgaaatatga
     3661 taaatcggca agacagatag ccaattagtg tcacgatcaa atcccaagct ctctttacat
     3721 ccgattgcac tggcataatg attacgaaac gtttgtggca ccgctttttg agggcccgtt
     3781 gtccctgatg taaacataat cgatgcaatg tcatctaaat taaatgatgt atttaatatg
     3841 ttggacggcg actctttcgg caccacagtt tcattcgatg tttcatattg gatacccatt
     3901 gtgttgtcca acaaactgtt cgttgtaata tcccttccag cgaattcaat atcatccagc
     3961 gatacaattt gaaaccctcg taattccagt ggcaaggtac aaaaaatcaa ttgtacatcg
     4021 attgacttca tctgattcgt catctcatta ggtgtcaacc ttgtattaat catcgcaatt
     4081 tcaatatttg ccaaccaaca tgcatgtatt aaaatgatcg attgaatcga attatctatg
     4141 tatagcccaa cacgagattg ttgataagcc ttgagtcttt tagccaatag actcgcttca
     4201 cagtataaat tttgataagt ataagattct tgaccgtctg ttatcgcaat atgatgtcca
     4261 ttttgttgtg cttgtttata taaccaaaag tccatgcgtt attcctccaa aatcatttac
     4321 attataatta taacgatttt atgacattct agcagtggtt atgtttaaaa atataaaaaa
     4381 gtagacgaat tgatgcattg atatgattgt tataatgctc aatacatatc gttatatcat
     4441 tcgtctacta ttatcagtta tttttattta attttagtgt cattctgtca ttttgatgtg
     4501 gtgatttacc cattgttgcc acatcatctg caatgtcaat tggtatacgg ttcatgtctt
     4561 gtaatgcact taaatggaat acttcatcat ctaaattttc aatgagatat acataatatg
     4621 ttaccttgtc ctttttatat tttaacgttt tccaaaagtc cggcttgcaa ttcaatacat
     4681 tatccggaat atattcaata aataagtaac gtttgctgcc tactttgtct atgaaatatt
     4741 ttgcagtgcc tttttctata cctcttatat gtgcatagtc tgctgaaaag taaatactac
     4801 ctattgtttc attatgttgt tgtatttcaa atcgttggcc tactatttta ttatttgtgc
     4861 tacaactact taaaaaaatc agtagatata gcattaaaca tattttcatc cccttgaatt
     4921 ttaaaaactt tttcaaaagc aacacctcta aaaataaata caatatatta tagcaaatag
     4981 aaattattat tttgttaaat tatgttgtca tgttcgtgtc attttgagtc gtaatac
//
LOCUS       DAKTGS010000164         4955 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.168, whole genome shotgun sequence.
ACCESSION   DAKTGS010000164 DAKTGS010000000
VERSION     DAKTGS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.168"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            56..370
                     /locus_tag="O0286_002562"
     CDS             56..370
                     /locus_tag="O0286_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0170899.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            370..1662
                     /gene="arsB"
                     /locus_tag="O0286_002563"
     CDS             370..1662
                     /gene="arsB"
                     /locus_tag="O0286_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCY0170900.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            1680..2075
                     /gene="arsC"
                     /locus_tag="O0286_002564"
     CDS             1680..2075
                     /gene="arsC"
                     /locus_tag="O0286_002564"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCY0170901.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
     gene            complement(2370..3224)
                     /locus_tag="O0286_002565"
     CDS             complement(2370..3224)
                     /locus_tag="O0286_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0170902.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(3500..3724)
                     /locus_tag="O0286_002566"
     CDS             complement(3500..3724)
                     /locus_tag="O0286_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170903.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            3923..4393
                     /gene="sigS"
                     /locus_tag="O0286_002567"
     CDS             3923..4393
                     /gene="sigS"
                     /locus_tag="O0286_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCY0170904.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            4506..4949
                     /locus_tag="O0286_002568"
     CDS             4506..4949
                     /locus_tag="O0286_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0170905.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
BASE COUNT         1651 a          799 c          697 g         1808 t
ORIGIN      
        1 acatagacat tcatctatat agatgataat ctatttaacg tctaggaggt atgttatgac
       61 gtataaagaa ctagcaacat ttttaaaagt tttatcagat tcaagcagat tagaaatact
      121 agatttactt tcttgtggag agttatgcgc ttgtgatttg ttagcacatt ttcaattctc
      181 tcaacctaca cttagctatc atatgaaagc attagtaaaa accaacttag ttacgacacg
      241 aaaaatcgga aataaacatt tataccagct caatcataat atttttgagt ccgtaattaa
      301 taacttgtct aaggttcata cctctaacca acgatgtatt tgtcataacc ttaagactgg
      361 tgaatgctaa tgatgacaac tttagcgaca ctcatttttc tagtaacttt attatttgta
      421 ttatggcaac ctaaaggcct agatattggc attaccgcac taactggtgc ctttattgct
      481 gttattactg gtgttgtaag tttttccgat gttttcgaag taacaggtat tgtttggaat
      541 gctactttga cttttgtctc agtcattctt atttcattaa tattagataa agttggatta
      601 ttcgaatggt cagctattca catgcttcat gcttcaaaag gcaatggttt aaaaatgttc
      661 gtttatatca tattattggg tgccattgtt gctgcatttt tcgcaaatga tggcgcagcg
      721 ttaatcttaa cgcctattgt attagcgatg gttaaaaata taggttttag taagcgggcc
      781 atattccctt ttattattgc gagtggtttt atagctgaca caacttcttt acctttaatc
      841 gtgagcaatc tagtgaatat tatatctgct gattattttc atgtaggatt cgttcgatat
      901 tttagtagaa tgattatacc taatttattc tcgcttttag caagtattat agtattgtgg
      961 ttatatttta gaaaagcgat acctaaaacg tttgatgata ataatataaa gcatcctaaa
     1021 gatgccatta atgatttaaa gctatttaaa atttcatgga ttgttctagt tatattactt
     1081 ttcggctatc taatcagtga atttactaaa attccggtat caattttcac tggaatcatt
     1141 gcttttattt ttctaatgtt ggctcgtaaa tcaaatgctg taaatattaa gcaagtcatt
     1201 aagggcgcac cttggaatat agtattattt tcaattggta tgtatatcgt cgtattcggc
     1261 ttaagaaatg ctggcattac tttaatattg gctaaaatat tagaatatat ttccaattac
     1321 ggtctattta gcactatttt gggaatgggc ttcatttcag cgtttttatc atcaataatg
     1381 aataatatgc ctacagtttt aatagatgcg attgctattg gtcaatcaaa tgtccatggc
     1441 atgttaaaag aaggcctaat ttatgcgaat gttatcggtt ctgatttagg tccaaaaatt
     1501 acaccgatag gctctttagc tacattactg tggttacacg tcttaacaca aaaagatgtt
     1561 aagatttctt ggggcacata ctttaaaact ggtatcatca ttacaattcc agtactattt
     1621 ataaccctca tagggttgta tctaacactt atcatatttt aattaattag gagattgtta
     1681 tgactaaaaa aacaatttat tttatatgta caggcaactc atgtcgaagt caaatggctg
     1741 aaggttgggc taaacaaatc ttagcggatg attggaatgt atattctgct ggtatcgaaa
     1801 cacacggtgt taatcccaaa gcgatagaag ctatgaaaga agtaggcatt gatatatcaa
     1861 atcatacatc agatttaatc gataataata ttattaaaaa ttcaaattta gttgttacat
     1921 tatgtagtga tgcagacgta aattgccctt ctttaccaac aaatgttaag aaagaacatt
     1981 ggggatttga tgatcctgca ggcaagcctt ggtcagagtt ccaacgtgta agagatgaaa
     2041 ttaaaatcgc aattgaaaat ttcaaatcac gatgagagtg ggacagaaat gataaaaaaa
     2101 cactaatgat ttattatgta gtggttcttt atcattagct ataactaatg tgtacttaaa
     2161 aataggaata catgagtaaa actcatgcat aagaaatact aatttctaaa gaaaaagtaa
     2221 ttctttatcg atgtcctgcc ccacttgcat tggtgggatt atcaatcaat gtgttttctc
     2281 taatgcaaaa agacgctttc tatcaaaaca agatagaaag cgtcttttta gtatgtccga
     2341 gtcataaaaa caactcgtag cttatcaaat tacttattca aatgtttact gtcatcttta
     2401 tacacaaagt atttgaagta ttttccttca gtcttcatgt tcttataaaa gtcagcgata
     2461 attgttgcat tactttctgc ccacttaata tctgtagcat attgatgttc tcctggattt
     2521 tttggattcc atctcatact atacaatgta ttttgatctg tgcttgataa gaagtgctta
     2581 tgaatgaaat cagcaccgcc tgaaatagct ttttcaggtg tatcccaacc atgcttttta
     2641 gcatattctg cacctgtttt aattgggtct ttatcaaggg ctcctactcc atagaaattg
     2701 tagtactttt tgccatcaat ttcgactcca ttagctaatt cacttttaac tgcgccagtt
     2761 tctaataatg catgtgaaat taaataaact tcgttaacgt gcttatcttt agcagctttt
     2821 aagaaatcat ccgtatgttt caataacgtt ggtctatcta ctaacatacg tttaattcta
     2881 tttttatcaa tcccttgata ctttgataaa tctaaaaatt gatatttttg cttttcatta
     2941 tcgataaaag taccgctatc cattgcactt ttaatttcag ttgcagatgc atctctccat
     3001 gcatcattct ttttatttga tacctgttga ctcgtataat tatttatttg tttctttgct
     3061 gcatcgttta atgtaacatt taacttttca atcttaatgt cggatttaac atgtttgaaa
     3121 aatatctgat cggatatcat tgagaaaaat aaaaatgaga caacagcaaa tatgacaaca
     3181 agtcctatta ttccaaaaat agaacctttc ttgtgtttat tcatatccac acctcttagg
     3241 tcattgttgt tatacattca gtttgactac gataataaag tttaacatta ttaaaaccgt
     3301 ttaacaattt tatctatgca ttgttacata ttcataaata aacagtaatg taaatgaaac
     3361 gttttaaacc atttatcaac attactttac gttgatctac gaatcataaa tgttttatct
     3421 ttttgtataa cctttgaaaa cctttatatc aataatctta ttattcacgt attaaaaaac
     3481 aaaaaattct tttgttcgtt tacttagttt cttttttata atccatcata ataaaacttg
     3541 ctgcatttga tctattttta taatagtact gatttaaatc aatcgtgcct tcaatttgac
     3601 catcacgata catcatttca ttagtgaagt tatttatcat cacgaaatca gcgcgatctc
     3661 ttataacatc taaatcgtcg tttcgtgcaa aaaaatgttc taaatttaaa tgtgcgtaat
     3721 ccattgttac ctcccgatta caaattttgt tctatctact atttagcaaa gtaataatac
     3781 atcatatcac attaaattaa ttacttatgt cttatattta acacttaatt tagcgattac
     3841 gcaaatgaat gatttttcta aatattttat tctaacttta tccggaaata tttgttatac
     3901 gtataacttg gagaatgact acttgaaatt taatgacgta tacaacaaac accacaaaat
     3961 catacaccat cttttaaaaa aatataatat tagctataat tatgatgagt attatcaact
     4021 actcttgata aaaatgtggc aattgagtca gatatataaa ccctcaagca agcaatcttt
     4081 atcctctttt ttattcactc gattaaattt ttaccttatc gatttattca gacaacaaaa
     4141 tcaattaaaa gatgtcattt tatgtgagaa taattcacca acattaactg aacaaccaac
     4201 atactttaat gaacatgacc ttcgtttaca agatatcttc aagcttttaa atcaaagaga
     4261 aagactatgg ctcaaactat accttgaagg atacaagcaa tttgaaattg ctgaaatcat
     4321 gtcattatcg ctttcaacga ttaaattaat taagatgtcc gttaagcgta aatgccaaca
     4381 taattttaat tagtttattg tgaaatttcg gtcgatgtgt taatgatgtt gaaacaccct
     4441 aacatcttca tataaattta ttttaaccat taaattaaca acttattaaa cgaggagtac
     4501 tatatttgca agacaattct actaaatatc tactttatat ccaaactgct acttcaaacc
     4561 atctcgaaac aaattgtgtc tttttacatt gcgattacat tcttaaagtt ccaattaaca
     4621 aactcgtttc atattatgcg aaattgcatt tatcatcaca aagtgtgcta attgagactg
     4681 caaaaaacat actaaatatt aataaactgg ttcctattta catcaaccca aaaactatac
     4741 tttttccgtt aaaacataaa cgtgcaccga tacaaattta tatcaatgca cattatattg
     4801 ttggtatgac tgccatagag aattcaacat taatacattt tcaagaaggc attcagctag
     4861 aagttgatga gccattttct ctagtttcaa aaaaatgtca tgaaagttta gctttgaagc
     4921 acttcattga aaatactatt tcgaattaaa tttaa
//
LOCUS       DAKTGS010000165         4713 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.169, whole genome shotgun sequence.
ACCESSION   DAKTGS010000165 DAKTGS010000000
VERSION     DAKTGS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.169"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..840
                     /locus_tag="O0286_002569"
     CDS             <1..840
                     /locus_tag="O0286_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0170906.1"
                     /translation="QKSTIRLYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEA
                     FKQLFNKDVHFNTFEVNTSLQSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTT
                     ITSSKCLVLFTSYKMMHMVQDMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILL
                     GTSTFFEGFDFQANGIKCVMIAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFR
                     QGLGRLIRNENDRGIIVSFDDRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQK
                     KKK"
     gene            1162..2454
                     /gene="asnS"
                     /locus_tag="O0286_002570"
     CDS             1162..2454
                     /gene="asnS"
                     /locus_tag="O0286_002570"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCY0170907.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            2782..3468
                     /locus_tag="O0286_002571"
     CDS             2782..3468
                     /locus_tag="O0286_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0170908.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            3458..4117
                     /gene="nth"
                     /locus_tag="O0286_002572"
     CDS             3458..4117
                     /gene="nth"
                     /locus_tag="O0286_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCY0170909.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            4122..4463
                     /locus_tag="O0286_002573"
     CDS             4122..4463
                     /locus_tag="O0286_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170910.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
BASE COUNT         1772 a          622 c          840 g         1479 t
ORIGIN      
        1 caaaaatcaa caatacgttt atatgtgaaa gattacgctg taaaagatgt attaacgaaa
       61 caagtattgg aaaaatttaa atcgctcatt tttatatctg gtactttaaa atttaatcat
      121 tcgtttgaag cttttaaaca gttattcaac aaagatgttc actttaatac atttgaagtt
      181 aacacgtcgt tacaaagtgc aaaaaataca agtgtattta taccgagtga tgtagcatct
      241 tatcaatata aaaatataga tgagtatgta gcatcgattg taagctatat tattgaatat
      301 acgactataa cgtcatcaaa atgtttagtc ttatttacga gttataaaat gatgcatatg
      361 gtacaagata tgctaaatga attgccagaa tttgaagatt atgttgtatt aacacaacag
      421 cagaaccaaa attataaaat agtacaacag tttaataatt ttgataaggc catactgctt
      481 ggtacgtcaa cattttttga aggttttgat tttcaagcaa atggtattaa atgtgtgatg
      541 atagcgaagt taccgtttat gaacaagcat aatgcaaaat attggcttat ggattctgaa
      601 ttcacttcaa ctttcaagga atatgtatta cctgatgcag ttacacgttt tagacaagga
      661 cttggaagat taattagaaa tgaaaatgat cgcggcataa tagtttcatt tgatgatcga
      721 ctcataaaca gtaattataa aaactttttt gaacaaacac ttgaaaatta tcgtcagaaa
      781 aaaggcgaca ttcaacagtt tggaaaatta ttaagacaaa ttcaaaaaaa gaaaaagtga
      841 aatacaaagt aaaattcttg tgacatgaca tgtgctatgt tctgttacaa gaatttttgt
      901 ttggtcgtca aaatttgagt tttataatta aacttgaatg ttagcacaaa aaacgacctt
      961 catactaata atattgaggc tttattatat attttgtctg aaactgatga ttgtattgtc
     1021 ttaatataaa ttaatgttca aatctgtgcg taaatcatta ccatttacac gttatctttt
     1081 agtctagttc tagaacttga aagcggattt tgttaaaatg aatgagtatt gtataacgaa
     1141 aaaataaata ggagaatggt tatgaaaaca acgattaaac aagcgaaaga tcatttaaac
     1201 caagacgtta caattggtgc ttggttaaca aataaacgtt caagtggtaa aatcgccttt
     1261 ttacaattac gtgatggaac aggctttatg caaggcgtag tagttaaatc agaagttgat
     1321 gaagaggtat tcaaacttgc gaaagaaatt actcaagaat catctctata cgttacaggc
     1381 acaattacag aagataatcg ttctgactta ggatacgaaa tgcaagtgaa atcaattgaa
     1441 gttatttcag aagcgcatga ctatccgatt acacctaaaa atcatggtac agaattctta
     1501 atggatcacc gtcatttatg gttacgttct aaaaaacaac atgctgtaat gaaaattaga
     1561 aatgaagtta ttcgtgcaac gtatgaattt ttcaacaaag atggatttac aaaggttgat
     1621 ccaccaattt tgacagcaag tgcgccagaa ggtacaagtg aattattcca tactaaatac
     1681 tttgatcaag atgcgttttt atctcaaagt ggtcagttat acttagaagc tgcagcaatg
     1741 gcacacggaa aagtattttc atttggtcca actttcagag ctgaaaaatc aaaaacacgt
     1801 agacacttga tcgagttctg gatgattgaa ggagaaatgg ctttcacaaa tcatgctgaa
     1861 agtttagaaa ttcaagaaca atatgtaaca catgttgtaa aatcagtttt agaaaattgt
     1921 aaactagagt tgaaaatttt agagcgtgat acatcaaaac ttgaaaaagt tgcgacacca
     1981 ttccctagaa tttcatatga tgatgcaatt gaattcttaa aagcagaagg ctttgatgat
     2041 attgaatggg gcgaagattt tggtgcgcca catgaaacag ccattgctaa tcattatgat
     2101 ttaccggtgt ttattactaa ttatccaact aaaattaagc ctttctatat gcaaccaaat
     2161 cctgaaaatg aagaaactgt attatgtgca gacttaattg cacctgaagg atacggtgaa
     2221 attattggtg gatctgaacg tgtggatgac ttagaattgt tagaacaacg cgttaaagaa
     2281 catggattag acgaagaagc atatagttac tacttagact tacgtcgtta tggtagtgtg
     2341 ccacactgtg gatttggttt aggtttagag cgtacagtag catggatttc tggtgttgaa
     2401 cacgttcgtg aaacagcgcc attcccaaga ttattaaacc gtttatatcc ataagtttta
     2461 gggtcgttac ttaaaatata tattatgata ataaaaagaa tcgtccagcc tatattaata
     2521 gctgggcgat ttattcttgg aaaatgaaga ttgataacgg cttatattaa tcatgaacaa
     2581 acttttgttt agtaactata tttggaaaat aaatagttca tctgattatc cataaagttc
     2641 attgttaaaa tctagtgtta aaaaatactg ttatttaaaa tttgtttgct actagttaaa
     2701 tatattaagc attttagtcg aaaattaaag aaaatgattt atactattaa gtaatggtta
     2761 aatttaaaag ggggcacgaa catggataaa tatcaattaa aagcaagacc tgtagtgata
     2821 cgaagagaat tattagacca ttacagtgac ttaggtttag atgagcaaga tttagtcatt
     2881 ttgcttaaat tgatttatgc ttccgaaaca tcaaataaac agccttcaat tgaattgctt
     2941 cagaagggct caactatgca accacgtgat attacaatgg ttattcaaaa tttgatacaa
     3001 cgtgaattgc tagaattaca agttcaaaaa gatgaagaag gtagatttac cgaatatatg
     3061 aatctagatc cttttttcga aaaattaagt catatattga agcaacaaag tatggaaacg
     3121 aaagagctaa atagtaaaga aaaatttaaa caattattta gagttttaga ggatacattt
     3181 gcaaggccgt tatctccata tgaaatagaa acgttaaatc aatggattga tgttgataaa
     3241 cacgatactg caattataca agcggcatta gacgaggcga atagtttgaa taaacttagc
     3301 tttaaatata tagatcgtat tttacttaat tggaagaaaa acaatgtcaa aacaattgac
     3361 gattctagaa aaataagaga aaaattcaat aaaccgaaaa tgacgcacac agttaaaacg
     3421 gtacctaaat ttgattggtt aaacggggag aaccttgatg gtaagtaaga aaaaagcatt
     3481 agaaatgatt gatgttatag caaatatgtt tccggatgca gaatgtgaat taaaacatga
     3541 taatccgttc gaattaacta ttgctgtatt attgtcagcg caatgtacag acgttctagt
     3601 aaatagagta acaactgagc tgtttaaaaa atacaaaacg cctgaagatt atttggcagt
     3661 cagtgatgaa gaacttatga atgatattcg ttctatcggt ctgtatcgta acaaagctaa
     3721 gaatattaag aaactttgtc aatctttgat tgatcaattt aatggagaaa taccacaaac
     3781 acataaggaa ttagaaagtt tagcaggtgt aggacgtaaa actgctaatg tagtcatgag
     3841 tgtagcattt gatgaacctt cattagctgt tgatacgcat gtagaacgtg tttctaaacg
     3901 cttaggtatt aatcgttgga aagataatgt gagacaggta gaagaccgtt tatgttctgt
     3961 catacctaga gatagatgga ataggagcca tcatcaactc attttctttg gaagatacca
     4021 ctgtttagcc cgaaaaccta aatgcgatat ttgtccacta ttagaagatt gtagagaagg
     4081 acaaaaacgt tataaagcta gtttgaaaga agcgtgataa catgacaaat attacaaaag
     4141 aggtatttga taatttagaa caagagattg atttatttgc caaaaataag acattaggtt
     4201 ctagtgaagc aaagccctac ttggatgaat accatagtaa aattattgat tattttaagc
     4261 aggttaatga cataactggc aatattgatt ttgataaatt aaatcaatat cctgttgtgc
     4321 caatgaattt taaagaaaga tatgattata tgattgaacg taaatatcat tttatgggct
     4381 atcgacaaat gaagaccttt aaaactgaat tgattaaaat gaatgctagt tatcaaacaa
     4441 ggttaaagaa taagcaggta tagacaagat tgatcatttc ctaatgttga aaatatataa
     4501 aactaattca tgcacacata tttataatta tgttaggtgt cgtgtcatta caacgtctta
     4561 atataaaaac tgtccatcta catcataatg gatatcttga gatatgcttt aagatatcta
     4621 tttcgaatcg taggtggatt tcttatatct atgattgtga aaatatgttg caaacatgtt
     4681 gatatttcac tgattaacgt acacagggaa tta
//
LOCUS       DAKTGS010000166         4693 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.170, whole genome shotgun sequence.
ACCESSION   DAKTGS010000166 DAKTGS010000000
VERSION     DAKTGS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.170"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            233..1198
                     /locus_tag="O0286_002574"
     CDS             233..1198
                     /locus_tag="O0286_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0170911.1"
                     /translation="MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEK
                     EFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKP
                     LLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIE
                     SNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRC
                     AITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVL
                     SKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
     gene            1295..1486
                     /locus_tag="O0286_002575"
     CDS             1295..1486
                     /locus_tag="O0286_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170912.1"
                     /translation="MYYLAYGLTTLDNCFKYYQLITQNEHFCYSQQEERESYQQKFQS
                     MINGLKIKIGIQSSNDDAQ"
     gene            1578..1907
                     /locus_tag="O0286_002576"
     CDS             1578..1907
                     /locus_tag="O0286_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170913.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
     gene            complement(1984..2343)
                     /locus_tag="O0286_002577"
     CDS             complement(1984..2343)
                     /locus_tag="O0286_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170914.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIFFLIAL
                     ILYILNINRGIKILLKK"
     gene            complement(2873..3723)
                     /locus_tag="O0286_002578"
                     /pseudo
     CDS             complement(2873..3723)
                     /locus_tag="O0286_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4235..>4693)
                     /locus_tag="O0286_002579"
     CDS             complement(4235..>4693)
                     /locus_tag="O0286_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170915.1"
                     /translation="VHELNNEYSIMSYWFEEPYESLTELQHLFDKHLLDESERRFIVE
                     DENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAIIYAFNILNMHKIY
                     LYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLLKSEYIL"
BASE COUNT         1744 a          611 c          681 g         1657 t
ORIGIN      
        1 aagaattatg aatttagttc ttattaatgt taaagatcat caattatttt gtcacattat
       61 cttgagagga ttaccttatg aggtagcctt ttatttgtca cctaatattg ctctaaggct
      121 aataatacat atataaaata aataattatc agaatatatt tagaaattga tatttaataa
      181 ttaaataaaa gtatgctaaa tatttataat ttacaagtga gctgatagaa agatgagtat
      241 aagcgaatat aaaaatagat acttagagtc actatataac acctttaaaa ctgagttaga
      301 aaacgaacgc actcgctatt ttatattaga taccaactat acaaatagtg atgatactga
      361 aaaggagttt acttgggatg taaaacagaa taataagatc agagaaggcg atttgttcat
      421 ttttagacgg ccaactaatt tatctcaaat tgctcaacaa ttttatttct ttggagctgg
      481 gaaaatagaa aaaattgagc gaaaaaataa tgtagcaacc gcttacattt caaaaccctt
      541 actttttgtg gatcgtgttt taaaagataa tattgaaaat ttaaaatggg agttcaaaga
      601 aagaattaaa gaagagtggg agcagttttt ctttaaaaat aaaatcagta atattacaaa
      661 aaatgatttt ttaaaactac taagtttgag tagaaatgta gtggaaatag aaagtaatta
      721 tattaataat gatatttcgc taacttttaa acaaatggct gatatggcaa ctcgaataaa
      781 gaaaaatatt tactacgtag ggaatcatga agcatttgtt caagctaagg gaagtgctca
      841 ttttgagttt tcaaaaagga ttaaacaaaa ttatagttat agatgtgcaa ttaccgggat
      901 taaaactaaa gactttttag tcgttacaca tattattcct tggcatgaaa atgagtttat
      961 tagactagat ccttcaaatg gtatttgttt gtctttattt ttagcaaaag catttaagaa
     1021 aggctttatc actttttcta acagttatag agttgtttta tctaaagaag cagagaaaga
     1081 tgctgcttta tatgaagaat taaagattta tgaaaatcaa aaaatcgaac taccagattg
     1141 tcagaaacct aatttgaaat atttagattg gcatagagaa catattttta aaaattaatt
     1201 ttgggagagg ttgtatgaca gatattagtc aattagtaga ggtgccatac atctttcatt
     1261 agtagattga aaaagagcac ttgaaacaga taatttgtat tatttagcct atggattaac
     1321 aactttagat aattgtttta aatattacca attaattaca caaaatgaac atttttgcta
     1381 ttctcaacaa gaagaaagag aaagttatca gcaaaaattt caaagtatga ttaatggttt
     1441 aaaaattaaa attggcatac aaagttcaaa tgatgatgcg caataagcta caaagtttga
     1501 ttttttaatt gaacgttata tgtttttaaa agaagaaaga gcttcgaatt aatatcttaa
     1561 tggaggaaga ttagtaaatg gctagaacgt ataatgtgat taaatatgtc attaagaaaa
     1621 ataaggatga tcttaaaata catggattaa gattatatga atttaatata aaagatgaaa
     1681 aagctataaa tattagccaa cccatttttg tgaacagagc aacattgatt gagaaaatac
     1741 aaaatggtga agcatttacg cacgccttta gagtaaatca caatacttat tatgcaggcc
     1801 aattaatttc tttatcattg aataaaaatg gttatattaa taacaacttt gaggcctcac
     1861 gagatattat tagaaacatt gaagtaagta ttttagacta tgaataaagg taatactgat
     1921 agaatacatt acaattaaaa aagggagtat aggactccct taataagttg atatataagt
     1981 gacttatttt tttaatagaa ttttaattcc tctgttaatg ttcaagatat ataatattaa
     2041 agcaattaaa aagaatataa tagtaataat taaaagaatg ctaaatatta ataattgatg
     2101 atccaaaggt ttatcaaaga cttctttaga aaaaataaat gccaatctac ctataaaaaa
     2161 actaacccaa gtcataatat gattaatgaa tgcttttttg ccatgttctg atgttggttc
     2221 atcagcaagt atccatacaa ataaaggtaa aatgacaggt gcgaaaaaga tacttaaata
     2281 actcaatgct gataaaacgt aactattgtc atttttatgc atattactat ttgtattttc
     2341 cacttttttc accttcttta cttttcttta ctattataaa aacattaata gcgagctatt
     2401 acaacagtta taagagaagc ttacgatatt gtaagtatgt gttgttataa tcaatataaa
     2461 attaagaaag acctgagaat ttatattatc atccacaatc gaagtgagta gcgaaattta
     2521 ttggaaaagg tacttatatt gaagatattg ttaatggttc atttttaaaa agccaagacg
     2581 atttgaagtt aaaagcttct gacttcactg tcgaagataa ctataactac ggtgtactta
     2641 tatttccaga gaatctttct cttacagata ataataagaa attggaatct ttaataaaaa
     2701 ctatactatt tacaggtaaa cgatatcaat atacttattt taaaatttga tcttaataga
     2761 taaggatata atgatttaaa caatttaaga gatattattt tcacaaaaaa agaatgcttt
     2821 ttgttagagg cactgaaccc ttaaaatggg aacttaataa aaacactatg ttctaggctg
     2881 ctaattctct gtattttaca ggggataagt agcctagttt ttgttgaatt cgattattat
     2941 tatagttttt aatgtatttt cgacaatatc tattacaata tgattagagt tattaagctg
     3001 attattgatg taaaaagttt cagactttag cgaggaatgg aaactttcta tcggggcgtt
     3061 atcggcaggt gttccctttc gggacatact tctgataatg cctttttctt cgcataattg
     3121 ataataagca taagatgtat aaacgctgcc ttgatcacta tgtaatatac acccctcagg
     3181 tatatcgatt tgatttaatg tatcattaac taaacgttgg tcttgtttat catctatttt
     3241 atacgccaca atttctccgt tataaatgtc cattatcgaa gataaataca acatagaatg
     3301 accaaatggt aaataagtaa tatcggttgt taatacttcc atgggacaac tcgctttaaa
     3361 ttgtctttgt aataaattgt ccgttttata atacggttta cctattcttg tcgtcttttt
     3421 aggtctaact cggcagttca aatgatgctt ctgcatcact ctctgtactc tcttatgatt
     3481 aattggtgat gtataacatt gattaatcaa tgctgtaatc ttacgataac cgtaggtata
     3541 ttgtttacca acatgttgtg aaaatctata actttcccca tttcgatacc atcgccacca
     3601 atttttcact tgtgttctat ttctaatatt taattctttc ataatttctt ttgttggaaa
     3661 tcctgctgct ttcatttcaa ctgctttata ctttgtttct actgaataag aagctctttt
     3721 catagaaaaa aacacctccg tatgattcat tttaatatga attcaacgaa agtgttttta
     3781 tataattccc actattgggg tcagtctaaa attaaaattg ataacttttt taataaaata
     3841 gatacactaa ttttaaaaca aggtaaaaaa atagaattat taaaacaacg caaacaaggc
     3901 ttactacaaa aaatgtttgt gtaattctga taacgtccta ttatgttaat tgaaaataga
     3961 caaaaataaa ttgagagcat cccttaccgc aaagtgaagg atgctctatt tttgttgaaa
     4021 ttaatataag cttcattctg atagatagtc attaataatt gtttgcaaat cgttattaag
     4081 taccgttgtt acagcgtcat cattcataaa atcctcgtag attttatcaa gaatttattc
     4141 ctcttacaga cattcgcgag aagtccgttt taattttatt agaagtaatt caggtttgaa
     4201 cctacctaaa tgaatatatg agttatcttt tatactacaa aatatattca gatttcaata
     4261 atgacataaa ataggcatct ttatatttac ctttagtgta gaattgctct ttaagtaatc
     4321 cttctgtttt aaatccttgt gactcgtata tatgaatagc ttttttgtta tctgcatcaa
     4381 catatagata gatcttgtgc atatttaaaa tattgaatgc ataaattatc gctttttcga
     4441 atgcgaattt tgcataacct ttaccactga actcaggttt aataattatt tgtatttcac
     4501 aattacgatg gatgtaatta atttctacta attcaacaat acctacgact tgattttcat
     4561 cttcaacaat aaaacgtctt tccgattcat ctaataaatg cttatcaaat aaatgttgaa
     4621 gttccgttaa ggattcataa ggctcttcaa accaataaga cataatagaa tattcattat
     4681 ttaattcatg aac
//
LOCUS       DAKTGS010000167         4603 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.171, whole genome shotgun sequence.
ACCESSION   DAKTGS010000167 DAKTGS010000000
VERSION     DAKTGS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.171"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            195..716
                     /locus_tag="O0286_002580"
     CDS             195..716
                     /locus_tag="O0286_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCY0170916.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            complement(886..2052)
                     /locus_tag="O0286_002581"
     CDS             complement(886..2052)
                     /locus_tag="O0286_002581"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCY0170917.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            2306..3583
                     /locus_tag="O0286_002582"
     CDS             2306..3583
                     /locus_tag="O0286_002582"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCY0170918.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            4162..4419
                     /locus_tag="O0286_002583"
     CDS             4162..4419
                     /locus_tag="O0286_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170919.1"
                     /translation="MEIIIEIYMYLRDGIKEHFILKKVRKKFKKLNEINSDIQNMYAE
                     NQGLLETDKTIIDKVLSTDVTNSHDINELYLFLKNYLDKTQ"
     gene            4513..>4603
                     /locus_tag="O0286_002584"
     CDS             4513..>4603
                     /locus_tag="O0286_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170920.1"
                     /translation="MTDKNGHGIAITGIILFVINALQINNDLPI"
BASE COUNT         1552 a          701 c          877 g         1473 t
ORIGIN      
        1 atttaagatt tggtttacaa agtaagaggt tagcacgtgt agtaagatat gtaactgtat
       61 attgcagcaa agtgatagct ggtattacgt gtttatatac gtaattgacg acttatttat
      121 ttttaaaaaa tgtgaacatg aatattttct aatttaacaa ttaaggcgta tgattagaaa
      181 aaggggggat ttttatggag tataagagtt actatgattc gcctgtagga cgattggaac
      241 ttttgagtga tggggtaagt ctgaccgctg ttttgtttga aaatcaacaa ggtgatggta
      301 ctagggaaga aaatacgtct ttagcgatat ttagagaggc gacacagtgg ttagacgcgt
      361 attttaaagg tgacaatcct gaaatcacaa tacctttaaa gccaacaggt agtcattttc
      421 aacagtgtgt ttggaatgaa ttaagacagg ttccttatgg gactctaaca acgtatggtg
      481 ctattgccaa aaaagtaggc aagctgctta acaagccaaa aatgtcagcc caagcggttg
      541 gaggtgcagt cggaagtaat ccattatcta ttatcgtgcc atgtcatcgt gttgtcggta
      601 aaacgggtag cttaacaggg ttcggtggaa cgattaataa taaaattaag ctgttagagt
      661 tggagaatat cgatatgtca aagctttatg taccaaaaca tagcacgaag ccgtaacgta
      721 taaaggatcc aggcattagg aaaacttcaa taatgtgtca aataggcata atgaattcat
      781 tcaatacgaa gtgttataaa tagagaacag cagtaagacg atttcaatta gaagtggtct
      841 tactgctgct tttaggtttt attatatgtg ttttaaatac accgactact ctggtctgtg
      901 atattcacga acgttatttt caatatttga taagtagaaa atatgacgat cttcatgaac
      961 agcatcttgt tcttcgtcaa attcaacgtc atcaaaacgt ttgaagaatg tttcatatgc
     1021 atcaacagat acttcagtac ggttatttaa taatgcttta tgtgcagctt gatctaaatg
     1081 atctttgtag ccttcaacta atgtcgcact ataaaattca ccaactgaac ctgagccata
     1141 actgaataaa ccgattgttt caccagcttg taaatctcga ttttcaagta atgatattag
     1201 gcttaaatat aatgatccag tataaatatt accgacataa cggttataat ctacagcatc
     1261 ttcatatcct gaacgtaaac gctcttgagt tgtttcatca gcgttatcaa tgattgactc
     1321 taatgccttt ttacccattt ttgtaaatgg aacatggaag catagagatg cgaagtcagc
     1381 tagcgactta ccttgacgtt ttgcgtattc attccagctt tgttggaatg agcggatata
     1441 agcatcttta gataatgcac catcaactaa tggatattta tgtccagttg gacgccagaa
     1501 atcataaacg tcttcagtgt aagcaacagc atcttcattt aatgccaaaa tgcttggatt
     1561 atgtgcaata accatcgcaa ctgcgccagc accttgtgtt ggctcgccgc ctgaattcaa
     1621 tccataacgt gctgtatctg tagcaataac taatactttt tcattcggtc tagttgctaa
     1681 ataatcttta gctaattgaa ttgctggtgt tgcagcataa caagcttctt tcatttcaaa
     1741 gcagcgtgca aaaggttgaa tacctaataa gttgtgaatt tgaacagcgg ctgctttagc
     1801 agcatcaact gctgattcag ttgccacaat taccatacca attttctttt tgtcttcgtc
     1861 tgttataatg tccttagcag cgttagcgcc cattgaaacg atgtcttggt ttacaggact
     1921 aacagccatt tcagtttgac caattccaat taaaaatttg tttgggtcta cttggcgtgc
     1981 ttctgctaat ttagccatgt ctacatagta ctttggaacg taaaagttta ttttatcgat
     2041 acctattgtc ataaggtttt acatcctttc aaatttataa aagcaacact tgtatcatat
     2101 cagaatatgc taattttata caaagaaata tactaaattt aaaaatgatt tgtcgtttct
     2161 tgtaagcgtt ttaatttacc ttgttttatt ttaaagtcaa aacgacaatt attcaattga
     2221 attttaagac gaatgacgtt attttgtggt aaaaatagta gtgattgaaa tgttaaaaat
     2281 tatgtgaagt aaaaggagta cgtccatgca aaatttagat aagaattttc gacatttatc
     2341 tcgtaaagaa aagttacaac aattggttga taagcaatgg ttatcagaag aacaattcga
     2401 cattttactg aatcatccat taatcgatga agaagtagcc aatagtttaa ttgaaaatgt
     2461 catcgcgcaa ggtgcattac ccgttggatt attaccgaat atcattgtgg acgataaggc
     2521 atatgttgta cctatgatgg tggaagagcc ttcagttgtc gctgcagcta gttatggtgc
     2581 aaagctagtg aatcagactg gcggatttaa aacggtatct tctgaacgta ttatgatagg
     2641 tcaaatcgtc tttgatggcg ttgacgatac tgaaaaatta tcagcagaca ttaaagcttt
     2701 agaaaagcaa attcataaaa ttgcggatga ggcatatcct tctattaaag cgcgtggtgg
     2761 tggttaccaa cgtatagcga ttgatacatt tcctgagcaa cagttactat ctttaaaagt
     2821 atttgttgat acgaaagatg ctatgggcgc taatatgctt aatacgattt tagaggccat
     2881 aactgcattt ttaaaaaatg aatttccgca aagcgacatt ttaatgagta ttttatccaa
     2941 tcatgcaaca gcgtccgttg ttaaagttca aggcgaaatt gatgttaaag atttagcaag
     3001 gggcgagaga actggagaag aggttgccaa acgaatggaa cgtgcttctg tattggccca
     3061 agtagatatt catcgtgcag caacacataa taaaggtgtt atgaatggca tacatgctgt
     3121 tgttttagca acaggaaatg atacgcgtgg tgcagaagca agtgcgcatg catacgcgag
     3181 tcgtgacgga cagtatcgtg gtattgctac atggcgttac gatcaagatc gtcaacgatt
     3241 gattggtaca attgaagtgc ctatgacatt ggcaatcgtt ggcggtggta caaaagtatt
     3301 accaattgct aaagcttcat tagagctact aaatgtagag tcagcacaag aattaggtca
     3361 tgtagttgct gccgttggtt tagcgcaaaa ctttgcagca tgtcgcgcgc ttgtgtcaga
     3421 aggtattcaa caaggtcata tgagtttaca atataaatca ttagctatcg ttgtaggggc
     3481 aaaaggtgat gaaattgcta aagtagctga agctttgaaa aaagaacccc gtgcaaatac
     3541 acaagcagcg gaacatattt tacaagaaat tagacaacaa tagtaaatgg cgaatagcga
     3601 ttttaaagaa aagtttaagt cagtccatcg tagcgcttag ataaattaat atgaacaaaa
     3661 ataaaaactt ctatcacacg catacaggaa attgaagcgt tcgtgataga agttttttga
     3721 ttagtaaatc agtctaatga aaagtaaata acttcttcat gagacgaatt cattatagga
     3781 ttcaaatttg gaacgagttt cttattgtct cgattttaac agtcttagat tcttcaagtt
     3841 cctatcattt tgatatttgt taacaatatg aacagtagaa tgcctcactc cactcctttt
     3901 ttagcaattt cttctgcagt catcatttat tcatcatata agaatttaat ttctcatttc
     3961 ttatgacttg cttgtaagta tgaaagaaca acagcattgc atttaaatac aattattatt
     4021 aattattcca ctagcgttaa aaagaagtaa taataagata cttaagaata aatatctgaa
     4081 aatactaaaa attctgaaaa aacctttctt atttttaata tattgagtat aatgtttttt
     4141 gagagaggag atatgacatt tatggagatt ataatagaga tttatatgta tttaagagat
     4201 ggaattaaag agcattttat tttgaaaaag gttagaaaaa aatttaaaaa attaaatgag
     4261 attaacagcg atatacaaaa tatgtatgca gaaaatcagg ggctcttaga aacagataaa
     4321 acaataattg acaaggtctt aagcactgac gttacaaata gccatgatat taatgaatta
     4381 tatttatttc ttaaaaacta tttggataag acgcaatgat aactgtagct gttatagata
     4441 cgggtgtaga tatctaccat aataaattat acaaatatat aaatttatca aaaagttttt
     4501 gttgatgaat ctttgactga taaaaatggt catggaatcg ctataactgg gattatactt
     4561 tttgttataa atgctcttca aataaataat gatttaccaa taa
//
LOCUS       DAKTGS010000168         4554 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.172, whole genome shotgun sequence.
ACCESSION   DAKTGS010000168 DAKTGS010000000
VERSION     DAKTGS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.172"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            435..2240
                     /gene="glmS"
                     /locus_tag="O0286_002585"
     CDS             435..2240
                     /gene="glmS"
                     /locus_tag="O0286_002585"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCY0170921.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            2472..3254
                     /locus_tag="O0286_002586"
     CDS             2472..3254
                     /locus_tag="O0286_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170922.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            3322..4179
                     /locus_tag="O0286_002587"
     CDS             3322..4179
                     /locus_tag="O0286_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0170923.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
BASE COUNT         1607 a          631 c          890 g         1426 t
ORIGIN      
        1 agggggaaag ttcggcagta cagttaaagc gcctgtgcaa ataaatattt gtatttgaag
       61 attaaaggtt aatatatgag tggcctttat agagtgcaat atatgtattt gtagacgagg
      121 aggatagtga tcgaatagat cggcggatgc tatcccggat gtggctcatt cgttagctta
      181 ttaagtaaaa cattagggtg acttaatgga caaagttaat aagatcgcca gaaattgaat
      241 ataaaaaata ttaatatgga aagtacagtg tgagcaattt gtatagttgt aaaaataact
      301 atgcttaatt tgttatggat gaatgcgatg atagcatgtt cctatttata ttatgaaagc
      361 agattgtcaa tctaaattat cggcaataaa tcataattta cgcgtactat tccaatatgg
      421 aggaaaatgt cgttatgtgt ggaattgttg gttatattgg ctatgataat gccaaagaat
      481 tattattaaa aggtttagaa aaattagaat acagaggtta tgactctgca ggtatcgcag
      541 tagtaaatga tgataataca actgtattta aagaaaaagg tcgtattgca gaattacgta
      601 aagttgctga tagtagcgat tttgatggac ctgttggaat cggtcacaca cgttgggcaa
      661 cacacggtgt accgaatcat gaaaactctc atccacatca atcatcaaat ggccgtttta
      721 ctctagttca taacggtgtt attgaaaact atgaagagtt aaaaggtgaa tacttacaag
      781 gtgtatcatt catttcagaa acagatacag aagttatcgt tcaattagtt gaatactttt
      841 caaatcaagg actttcaact gaagaagcat ttacaaaagt tgtgtcatta ttacatggtt
      901 catatgcatt aggtttatta gatgctgaag acaaagacac aatctatgtt gctaaaaata
      961 aatcaccatt attattaggt gttggtgaag gtttcaatgt tatcgcatca gacgcacttg
     1021 caatgttaca agtgacaagc gaatataaag aaatccatga ccatgaaatc gttattgtta
     1081 aaaaagatga agttattatt aaagatgcag atggaaacgt tgtagaacgt gattcatata
     1141 ttgctgaaat tgatgcatca gatgctgaaa aaggtgttta tgcacactac atgttaaaag
     1201 aaattcatga acaaccagca gtaatgcgtc gtattattca agaatatcaa gatgcagaag
     1261 gtaacttgaa aattgatcaa gacatcatca atgatgttaa agaagcagac cgcatttacg
     1321 ttattgcagc aggtacaagc taccatgcag gtttagtagg taaagaattt ttagaaaaat
     1381 gggctggcgt accaactgaa gtacacgttg catcagagtt tgtctacaac atgccattat
     1441 tatctgaaaa accattgttc gtttatattt ctcaatcagg tgaaactgca gatagccgcg
     1501 ccgtattagt tgaaactaat aaattaggtc ataaatcatt aacaatcact aatgttgcag
     1561 gttcaacttt atcacgtgaa gcagaccaca cattgttatt acacgcgggt cctgaaatcg
     1621 cagttgcatc tacaaaagca tatactgcac aaattgcagt attatcaatc ttgtctcaaa
     1681 tcgttgcaaa agagcatggt cgtgaagcag atattgattt attgagagaa ttagcaaaag
     1741 taacaacagc aatagaagca attgttgacg atgcaccaat tatggaacaa attgctacag
     1801 atttcttaga aacaacacgc aatgcattct ttatcggacg tactattgac tataacgtaa
     1861 gtttagaagg tgcgttaaaa cttaaagaaa tttcttacat tcaagcagaa ggttttgctg
     1921 gtggagaact taaacatggt acaattgcct taatcgaaga aggtacacca gttgtaggtt
     1981 tagcaacaca agagaaagtt aatttatcaa ttcgtggtaa cgttaaagag gtagtagcac
     2041 gtggtgcaca tccatgtatt atttctatgg agggtcttga aaaagaaggc gacacttatg
     2101 tcattcctca tgtacatgaa ttgttaacgc cattagtatc agtggttgca ttacaattaa
     2161 tttcatacta tgcagcatta cacagagatt tagatgttga taaaccacgt aaccttgcta
     2221 aatcagttac tgtggaataa ttcacttttt tagaatcaat catgtattaa aattaaagta
     2281 tatggcaccc ttttagatta atcgactaga agggtgcttt tttaggtcga cttagctttt
     2341 acttcatctt aatttggcag aaatgcgtaa aaatgaagtg ttttatttat ttaaatagtc
     2401 tgacaattaa gggtgttatg ttaatatgat tttatgagaa gtatggagta gcaataaagg
     2461 ggtgacctcg catgttaatt caattagatc aaattgggcg aatgaagcaa ggaaaaacaa
     2521 ttttaaaaaa gatttcttgg caaattgcta aaggtgataa atggatatta tatgggttga
     2581 atggtgctgg caagacaaca cttctaaata ttttaaatgc gtatgagcct gcaacatctg
     2641 gaactgttaa ccttttcggt aaaatgccag gcaaggtagg gtattctgca gagactgtac
     2701 gacaacatat aggttttgta tctcatagtt tactggaaaa gtttcaagag ggtgaaagag
     2761 taatcgatgt ggtgataagc ggtgccttta aatcaattgg tgtttatcaa gatattgatg
     2821 atgagatacg taatgaagca catcaattac ttaaattagt tggaatgttt gctaaagcgc
     2881 aacaatatat tggttattta tctaccggtg aaaaacaacg agtgatgatt gcacgagctt
     2941 taatggggca accccaggtt ttaattttag atgagccagc agctggttta gactttattg
     3001 cacgagaatc gttgttaagt atacttgact cattgtcaga ttcatatcca acgcttgcga
     3061 tgatttatgt gacgcacttt attgaagaaa taactgctaa cttttccaaa attttactgc
     3121 taaaagatgg ccaaagtatt caacaaggcg ctgtagaaga catattaact tctgaaaaca
     3181 tgtcacgatt tttccagaaa aatgtagcag ttcaaagatg gaataatcga ttttctatgg
     3241 caatgttaga gtaaatattt tgcaaataat aagtaataat gacaaaattt aattaagata
     3301 aaatggacag tggagggcaa tatggataac gtaaaagcaa tatttttgga catggatgga
     3361 acaattttac atgaaaataa tcaagcatca acgtatacga aagatgtcat taatcaattg
     3421 agagagaaag gatataaagt atttttggca acaggacgtt cgcattctga aatacatcaa
     3481 cttgtacctc aagattttgc ggttaatggc atcattagtt caaatggaac aattggagaa
     3541 gtagatggag aaattatctt caagcatggt ttatcattgg ctcaagtgca acaaattact
     3601 aatttagcta agcgccaaca aatttattat gaggtatttc cttttgaagg taatagagtt
     3661 tctttaaaag aagatgaaac atggatgcga gatatgattc gtagtcaaga tcctattaat
     3721 ggcgtaagtc atagtgaatg gtcttcaaga caagatgcgc ttgctggtaa gatagattgg
     3781 gtaactaagt ttcctgaagg tgaatattca aaaatttatc tattcagttc taatttagaa
     3841 aaaataacag catttagaga tgaattaaag caaaatcatg tgcaactaca gattagtgtt
     3901 tcaaattcat caagatttaa tgcggaaaca atggcttatc aaactgataa aggtacaggc
     3961 attaaagaaa tgattgcaca ttttggtatt catcaagaag aaacgttagt tattggagat
     4021 agcgacaatg atagagcaat gtttgaattt ggtcattata cagttgctat gaaaaatgca
     4081 cgccctgaaa tccaagcatt aacttcagat gtaacggcat acacgaatga agaggatggc
     4141 gcagcaaaat atttagcaga gcatttttta gctgaataat aaaataggta gttatttatt
     4201 atttaattta caatagttga tgagtaatgt acaaagagca gtaaagttat tttctattag
     4261 aaaatgtctt actgctcttt tgtatgctta taaatatttg aatcatctat atttaattgg
     4321 acaaactcta tgagaataaa tattgttaaa actaataaga taggaaattc attgattttg
     4381 aataatattt cttgttttaa ggtttaacta ttgaattgta tacttctttt tttagtagca
     4441 acagatccgt ctttaaaaaa attcacacct attgactgtt tttgttcttg attatattct
     4501 ctatgaagtg ttaatccact ataaaatcaa ccggttcgtt tacatatttc ccca
//
LOCUS       DAKTGS010000169         4528 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.173, whole genome shotgun sequence.
ACCESSION   DAKTGS010000169 DAKTGS010000000
VERSION     DAKTGS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.173"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(92..247)
                     /locus_tag="O0286_002588"
     CDS             complement(92..247)
                     /locus_tag="O0286_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170924.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKFKIF
                     VDILKQI"
     gene            285..746
                     /gene="tsaE"
                     /locus_tag="O0286_002589"
     CDS             285..746
                     /gene="tsaE"
                     /locus_tag="O0286_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCY0170925.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            727..1389
                     /gene="tsaB"
                     /locus_tag="O0286_002590"
     CDS             727..1389
                     /gene="tsaB"
                     /locus_tag="O0286_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCY0170926.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1362..1826
                     /gene="rimI"
                     /locus_tag="O0286_002591"
     CDS             1362..1826
                     /gene="rimI"
                     /locus_tag="O0286_002591"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCY0170927.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1819..2844
                     /gene="tsaD"
                     /locus_tag="O0286_002592"
     CDS             1819..2844
                     /gene="tsaD"
                     /locus_tag="O0286_002592"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCY0170928.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2946..>4528)
                     /locus_tag="O0286_002593"
     CDS             complement(2946..>4528)
                     /locus_tag="O0286_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCY0170929.1"
                     /translation="VIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTISPLEAFIRPN
                     SHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMKLYACLRGMLS
                     ITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNILFLCPFLPVIS
                     FAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLKQGYTISKIKH
                     APQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLFHSIQKSYTTH
                     MNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELTHPLIADAVAN
                     DFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPGIVFTSMANAD
                     DVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAASESVLSFLHEK
                     SNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKANTRNAIELLKI
                     TSFPAKIYERAKDNVPKI"
BASE COUNT         1553 a          637 c          833 g         1505 t
ORIGIN      
        1 attgattaag cattttatta tactccttcc gtgaatggta tatatcactt taatagttag
       61 aattgtctaa aaatttgata aatttaattt actatatctg ctttagtatg tcaacaaata
      121 ttttaaattt tcttacaatt aactttgaat agataatctg tttcaaagat aagaaattta
      181 agttgaaatt tgttacaata taaaggttaa atataaagtt agatacgatt gaacaatgga
      241 taatcatttt tatgaatgaa aagcataata taggagaatc aacattgata aagataaata
      301 atttagatga aatgaatcaa tttgctatat ttttagttga gcaattgaaa agtggtgatt
      361 tgattttact taacggagat ttaggagcag gtaaaacaac gttaacgcaa tttataggaa
      421 aagctcttgg tgtaagacgt acgattaatt ccccgacatt taacatcatt aaatcatata
      481 ggggtaaaaa tttaaaattg catcatatgg attgttatcg cttagaagat tctgatgaag
      541 atttaggatt tgatgaattt ttcgaagatc aggcaattac tgttattgaa tggagtcaat
      601 ttataaaaga tttacttcca gcgacgcatt tatctattaa catttcaaca atatctgaaa
      661 atacaagaca aattgagttg ttcgcgcaag gagaacatta tgaacaaatt aaggaggcaa
      721 ttatccatga attcgctgct cattgataca tcgaatcaac cattatcagt agcattgatg
      781 caaaacgatg aagttttagc tgaaataact acggattcta aacaaaatca ttcagtgcaa
      841 ttaatgcctg cgattagtca gctatttgag cagagcaaaa tagctaaaca gcaattagat
      901 gcgattattg ttgctgaagg tcctggttca tatactggct tacgtatcgg tgttactgtt
      961 gctaaaacat tagcgtatgc gttagatgtt aaattatatg gtgtttcatc attaaaggca
     1021 ttggcagcaa caatcgatca tactgataaa ttattggttc ctgtgtttga tgcaagacga
     1081 caagcagttt atacaggcat attccagtgg caaaatggtc aattagtgac tattttagaa
     1141 gatcaatata tgtcgataga ggcattgcga gcttttttag aagatttaaa ccaaccattt
     1201 atttatattg ggaaagatac tgtaaaatta caagatgaat tacaaggtga agtgattgcg
     1261 cagctaccaa atgcatcagt catgtatcaa ttgctagata aaccttcaga cgtacataca
     1321 tttactccta aataccataa attagctgag gcggaacgaa attggatcaa cagtcaaaag
     1381 aacaattaaa cattagagag atgactaaag aagatgtgcc acaagtcttt gatcttgagc
     1441 gtcggagttt caatgacagt tcttggacaa ttgacgcatt ttatcatgaa atagagcaaa
     1501 ataactttgc gaaatatttt gtcttagaat ttgaacaaca aattattggt tatttaggtt
     1561 tatggattgt tatcgatcaa gctcaaatta caacagttgc aatcgatgat aattatagag
     1621 gctatggttt aggtcaaatg cttttaaaat acggaataaa ctatgcaagt cacacttgtg
     1681 atgtgatgag tttagaagta agagttaata ataaagtggc acaacatgta tatgaaaatt
     1741 taggatttca atatggtggt aaacgtaaaa attattatgg tgaaggtgag gacgcaatgg
     1801 ttatgtgggt gaatttaaat gactaaagat atattaatac tagctgttga aacaagttgt
     1861 gatgaaacaa gcgttagtgt tataaaaaat ggcagagata ttttatcaaa tacagtttta
     1921 agtcagattg aaagtcataa acgatttggc ggtgtcgttc ccgaagtggc aagtagacat
     1981 cacgttgaag gtataacagc aacaataaac gaggctctag gggatgccga tgtatcaata
     2041 gaagatattg atgccatagc ggttacagaa ggccctggac taattggtgc gttactaata
     2101 ggtgttaatg cagccaaagc attggcattt gcttacgata agccacttat tcctgttcat
     2161 catattgcag gacatatata tgctaatcac atagaagagc cattaacatt cccgctaatt
     2221 gcacttattg tttcaggtgg acatactgaa ttagtttata tgaaggatca tttatcattt
     2281 gaagtcattg gtgaaacacg agatgacgca gtaggtgagg cttatgataa agtggcacga
     2341 acaattggtt taaattatcc aggtggtcca caagttgatc ggttggctgc tgaaggtgaa
     2401 gatacttatt cattccctcg tgtttggttg gataaagata gttatgattt tagttttagt
     2461 gggttgaaaa gtgccgtaat caatcaactt cacaatcaac gacaaaaaaa tattccaatc
     2521 attgaagcta acgtagcaac gagctttcaa aacagtgttg tagaggtgct cacgtttaaa
     2581 gctattcaag cttgtaaaga atatggtgtt cagcgattaa ttgttgctgg tggcgtggcg
     2641 agtaataaag gattacgtca atctttagcg gatcaatgca aagtcaatga cattcaatta
     2701 actatcccaa gtcctaaatt atgcacagat aatgctgcaa tgataggcgt tgccggccac
     2761 tatttgtatc agcaaggtcg atttgctgat ttagcattaa atgggcacag caatatagat
     2821 ttagaagagt attctgcaga ataaaataaa cgtcagttat taacatgatt actatgaaca
     2881 ttattgaaac tgttcatagt cttctgtcat gtgatagctg acgtttttat gtttaaagtt
     2941 aaatgctaaa ttttcgggac attatctttt gctcgttcat atatttttgc tggaaatgaa
     3001 gtgattttta ataattcgat ggcattacgt gtatttgctt tgccaggctt aattttgtaa
     3061 tcaaaatgta tgttattatt ttctattacc tcattgaaat ggtaattttc ataacgttgt
     3121 tttaagagtt cagctaactc aatatcatgt gttgctgcaa taactctaaa gttagatttt
     3181 tcatgtaaaa atgatagtac tgattctgaa gcggcaattc gttcagttgt gttggtacct
     3241 ttaaaaattt catctataaa gcagtatatt ttttgattat ctggaatttc aacaatacgc
     3301 ttaatagatt ttaattcagc cataaaataa ctatcgcctg ataacacatc atcggcattt
     3361 gccattgacg tgaacacaat tccaggttga tatacaaatt tgcttgcggt aactgtttgt
     3421 atagccgatg caagaataat gtttattgct atggatttca taaatgttga tttacccgaa
     3481 gcattagacc cagttaacag tatattttgt gatagtgaga aatcattggc tactgcatct
     3541 gctatgagtg gatgtgttaa ctcagagaat actatgccat catttgaatc atcaatttgt
     3601 ggttcagtat aacattctag cgttctacga tacattgcta atgcataatg attatctaat
     3661 tcagctatgt aatcgaaaca atttttaagc tcattcatat gtgttgtata gcttttttga
     3721 attgaatgaa ataaaacata atcaagcatg aaaataagtt taactagttt aattaccata
     3781 gctccgatat cttcatcatt aacttcagct aaaacactcg ttaaatgacg tgctgttcta
     3841 aattgcttaa aattgacttc aggttgtgga gcatgtttaa tttttgagat agtatagcct
     3901 tgttttaaaa cattggatgc ataaaagata gattttaagt catcctcata tgtacgcttt
     3961 aaagttgctg ataaaatgat attaaatagg caactcataa gaaataataa aataccaact
     4021 tgtgaattaa taaatataac tgcaaatgag ataactggta aaaacgggca tagaaacaat
     4081 atattttgac gttttaccgg tgtgatttgg tctggaaatg ttggataaac agttttacca
     4141 atcaaagcta aatgatatgt tacgtttttt ctaaattcag cattatcatt aaataaacta
     4201 agtaatgatt tgttcgtaat tgaaagcatt ccacgtaaac aggcatatag cttcatttca
     4261 ccaatagctg ttaaattata attcatctta tgaaagattg cattcatatt taagtcggac
     4321 caagttttat catcaactaa tgaatgtgat tcaaattttt ccttgtagag cttatactca
     4381 tagtcgaaat gtgagttcgg tctgataaaa gcttctaatg gagaaatcgt ttgccataat
     4441 gtttccatag attttaatag acgttgacga ctcatgtatc gtccaacaat tcctattaca
     4501 gatgtaagta gaataattgc tataacaa
//
LOCUS       DAKTGS010000170         4521 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.174, whole genome shotgun sequence.
ACCESSION   DAKTGS010000170 DAKTGS010000000
VERSION     DAKTGS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.174"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            172..1209
                     /locus_tag="O0286_002594"
                     /pseudo
     CDS             172..1209
                     /locus_tag="O0286_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1260..2090
                     /gene="sph"
                     /locus_tag="O0286_002595"
                     /pseudo
     CDS             1260..2090
                     /gene="sph"
                     /locus_tag="O0286_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2328..3344)
                     /gene="lukG"
                     /locus_tag="O0286_002596"
     CDS             complement(2328..3344)
                     /gene="lukG"
                     /locus_tag="O0286_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCY0170930.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(3366..4421)
                     /gene="lukH"
                     /locus_tag="O0286_002597"
     CDS             complement(3366..4421)
                     /gene="lukH"
                     /locus_tag="O0286_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCY0170931.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         1528 a          725 c          671 g         1597 t
ORIGIN      
        1 aggtttatcc tttaaaacac gtatatattc gttaccattt ttggtaatta accatgtaat
       61 cttataacta taagtggcgt ctgtatttta tacaggcgtc tttttttata caattttcat
      121 gggtagcccg cctaccctta ttattttttg ccaattttga ggagggaacg catgaaaaca
      181 cgttgttacg atggtaaaaa atgacaatat gaatttaagt atgaaggaaa aagataccgt
      241 aagaaaggtt ttagaacaaa gcgtgaagct aattctgctg gactagacaa gttaaatgag
      301 ttaagaagtg gttttaatat agataactat ataactcttg aagaatactt cgaaaattgg
      361 attaaaacgt ataaacaacc tgttgttaaa gaaaatacct accgtcatta tagaaatgca
      421 ttacaacata tacaaaaaca taaaataggt aaaatggagt tatcaaagat aaatagacaa
      481 gtttatcaga aattcataaa cgattattca aaagaacacg caaaagaaac tataagaaaa
      541 acaaacggtg ctattcggtc agctttagat gacgcattat atgatgggct tatttttaaa
      601 aatcccgctt ataaagttaa ttataaagcc ggaaaaccta cgaagtcaga acaagaaaaa
      661 ttcatctcgg taactgaata tgaaatacta aaagatcacg tcagaaagaa gagaactcgt
      721 tcatcattag cgctattcat aatgatttgt acgggttgtc gtgtcagtgg tgcaagaaat
      781 ataaagattg agcatatcaa ccaagtgaaa aacactatat ttattgacga gcgaaaaacc
      841 gatacttccc ctagatatat cagtatcgct aaatctgata tgaaacacat tatggacgtc
      901 ataagtacat ttgcaattag ctatgatggt tacattttca aagaagccgg atctataatt
      961 aaccttcatg ctatcaataa tgctttgaaa tcagcctgta gagtcaataa tatatcaatt
     1021 attacatcgc acgcattaag acacactcat tgttcttatt tactagcaaa aggtgtatct
     1081 atacattaca tttctaaaag attaggtcat aaaaatatag caataactac atccgtgtat
     1141 tctcatttgt tagaagaaaa atttaatgaa gaggacaaaa aaacaactaa aattttagaa
     1201 agtatgtaat ttagggaccc attagggact ccaaacccaa taaatactgt tgttacaagg
     1261 tttctatgta tccaaactgg gggcaatata aacgcgctga tttaatcgga caatcttctt
     1321 atattaaaaa taatgatgtc gtaatattca atgaagcatt tgataatggt gcatcagaca
     1381 aattattaag taatgtgaaa aaagaatatc cttatcaaac acctgtactc ggccgttctc
     1441 aatcagggtg ggacaaaact gaaggtagct actcatcaac tgttgcagaa gatggtggcg
     1501 tagcgattgt aagtaaatat cctattaaag aaaaaatcca gcatgttttc aaaagcggtt
     1561 gtggattcga taatgatagc aacaaaggct ttgtttatac aaaaatagag aaaaatggta
     1621 agaacgttca cgttatcggt acacatacac aatctgaaga ttcacgttgt ggtgctggac
     1681 atgatcgaaa aattagagct gaacaaatga aagaaatcag tgactttgtt aaaaagaaaa
     1741 atatccctaa agatgaaacg gtatatatag gtggcgacct taatgtcaat aaaggcactc
     1801 cagagttcaa agatatgctt aaaaacttga atgtaaatga tgttctatat gcaggtcata
     1861 atagcacatg ggaccctcaa tcaaattcaa ttgcgaaata taattaccct aatggtaaac
     1921 cagaacattt agactatata tttacagata aagatcataa acaaccaaaa caattagtca
     1981 atgaagttgt gactgaaaaa cctaagccat gggatgtata tgcgttccca tattactacg
     2041 tttacaatga tttttcagat cattacccaa tcaaagccta tagtaaatag tgctcaacta
     2101 actaataact tgcttcgttc taaaaggacg aagcgagtta tattgttaaa atttgaattg
     2161 acttacattt taataaaatc atcttaacaa ctttaatttt tcattaatac aagtctttac
     2221 tctacactca aacaagattc atacactgca cgtcataata aatctatcta ttcaaatata
     2281 aataaaagtt acctactaca ttctatgtag caggcaactt ttattactta tttcttttca
     2341 ttatcattaa gtacttttac aaacttcaca ttatgtgtct tccaatcaac ttcatataat
     2401 gctgataatt tttcttcttt tttatctaca tggttttcac cagaccaata gccccagaaa
     2461 ccatggcgat tccaatctat tttaaactca tccattgatc ttttataatg aacaacaaat
     2521 tgtgatttac ctttgtcttt tttatcatgt gacataacag ctaaaaattc tggattaaac
     2581 ccttcagaca cagttacagg cattttgtct ttaggtgtga aattatcttt cgcccataaa
     2641 tttccatttc gtgttaaaga aaaaatttca cttttagttc tattatcact atcattagtt
     2701 aattgtctcg tatggtcatg tcccatatta tttatcaaat gtgcttctac tttccaacct
     2761 acacctttat gtgacgtaga ttgatcaagt aatgtacgat atgatggttg ttgataacta
     2821 atcgtctctg aataattact ctcttttgta atatttccag ttaagcctcc acgattaatc
     2881 gaaaaatctc cacctgtttt ataaccatac gtatatttaa cttctcttga ttcatcctga
     2941 ttttttggtg caaagtcagt cacatttgta ttgttgttgt catcaacatt ttgaattgaa
     3001 actgaataag atccaggcca tcttaatgta ctattccaat aaccatttgg gtctaaaatt
     3061 ctcaaaccac taccaattgt accttttgct ttaataaata ctgtttcttt atcataatta
     3121 ggttcagtta agaaattaaa ttgtaagctt tgagtaatat ttttttgact atcacttgtt
     3181 gtagctgtac gtgtatacat tttagtatca ccatcaagat tcttctcaga aacttgtttg
     3241 atttcagaat taatctttgc aaatgaagta gctggtaata cagtgaaagt agtcgatagt
     3301 gctaacgtac aaattgtgat atttttacat agttgtttaa tcattagtaa tccgcccttt
     3361 caatattatc cttctttata aggtttattg tcatcagaat atttatcaac gactttaact
     3421 gttttatttt tccaatcaac ttcataagtg acaattaatc tttgaccatc tttatttttt
     3481 tctaaaattg gaggtgcata atgtattcca ggtctgtttt tcaaaatatc ttgatttcgt
     3541 gtgtatgtta cttcaaattg cgttttctca tttgactttt cattagataa ataagttaaa
     3601 aattctggat taaagccact tcttactaat gctgggtatc tatattttga agcaaagctt
     3661 agttcagggt tttctacagt agcaattctc gtatttctat agaataataa ttcatcattt
     3721 ctatttttca cttctccacc atacttcaag tcattcgcaa taactgacca gtgtacatgc
     3781 cagttattat ttttaccgct ggcaattgtg tcataatttt gctgattata actaatcgtt
     3841 ttggagtagc tatttgatga agttcgtcca ataccttttg ttgaatcgaa tttaccacct
     3901 gagctatatg aaaatgtact gtctactttt gcagttgaaa ttttattttt cggcaattgg
     3961 tctaatattt cagttttacg atttcttttt acttgaaaat ctacatggta ctcacttgga
     4021 tactttaacc aatttgaatt tttttcttct ttatgagatt caaactttaa atttgaatga
     4081 attgagcctt gttttttaac aagtaataca ttcttgtcat aagttggatc atcgataaag
     4141 tcgaattgta aattttggag tatatttgtt ttctcatcat atactgtttc agttcgtttt
     4201 gtgattttac cgtttttccc aatatcatcc ggtgctgttg aatttttatc tttattagta
     4261 acattacgtt tgtctttttg ttgagactta tcaacatgtt ctttcttatt ttggtcttga
     4321 gagtctttat gagctgaatt tgcttgagtc gttgctgctg ataacaataa aattgcacat
     4381 gataatgatg acgctattaa aacacgtttt ttatttttca ttgttataac cttctttcgt
     4441 atgattgata tttgttgata tgtatcgaca tgtgaataat atcacaaaaa cagagaatat
     4501 atatttaact atttattaaa t
//
LOCUS       DAKTGS010000171         4517 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.175, whole genome shotgun sequence.
ACCESSION   DAKTGS010000171 DAKTGS010000000
VERSION     DAKTGS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.175"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            42..1388
                     /locus_tag="O0286_002598"
     CDS             42..1388
                     /locus_tag="O0286_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0170932.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            1398..2288
                     /locus_tag="O0286_002599"
     CDS             1398..2288
                     /locus_tag="O0286_002599"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCY0170933.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            2414..3199
                     /locus_tag="O0286_002600"
     CDS             2414..3199
                     /locus_tag="O0286_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0170934.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            3241..4104
                     /locus_tag="O0286_002601"
     CDS             3241..4104
                     /locus_tag="O0286_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0170935.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            4091..4501
                     /locus_tag="O0286_002602"
     CDS             4091..4501
                     /locus_tag="O0286_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0170936.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
BASE COUNT         1649 a          594 c          835 g         1439 t
ORIGIN      
        1 actttataat agtaaagatg aacatataag gaggccaaat catggcaaaa catccattcg
       61 aacaatttaa tctagaatct agtttaattg acgctgtgaa agaccttaat tttgaaaaac
      121 caactgaaat tcagaatcga attattccaa gaatactaaa gagaacaaat ttaattggtc
      181 aatctcaaac gggtacaggg aaatctcatg catttttatt accattaatg cagttaattg
      241 atagtgaaat aaaagaacca caagcaatcg tagttgcacc aacaagagaa cttgcacaac
      301 aactatacga tgcagcgaac catttaagcc aatttaaagc tggtgtttca gttaaagttt
      361 ttattggtgg tacagatata gagaaagata gacaacgttg taatgcacaa ccacaattga
      421 ttataggcac ccctactaga attaatgact tagctaaaac gggacattta catgtgcact
      481 tagcatcata tttagttatt gatgaagcgg atcttatgat tgacttagga ttaattgaag
      541 atgtagatta cattgctgca agattggaag ataatgcaaa tattgcggtg tttagtgcta
      601 caatcccaca acagttacaa ccatttttaa ataaatattt aagtcatcca gaatatgtag
      661 ctgtcgacag taaaaaacaa aataaaaaga acatcgaatt ctatttaata cctactaaag
      721 gtgcagctaa agttgaaaag actttaaatt taattgatat actaaatcca tacttatgta
      781 ttattttctg taatagtaga gataatgcaa atgatttagc acgttcacta aatgaagctg
      841 gtattaaagt tggtatgatt catggtggct taacgccacg tgaacgtaaa caacaaatga
      901 aacgtatacg taatttagaa ttccaatacg ttattgccag cgatttagca tctcgtggta
      961 ttgatattga aggtgttagt catgtcatca attttgatgt gccaaatgat attgacttct
     1021 ttacgcatag agtcggacga actggtcgtg ggaattataa aggtgtagca attacgcttt
     1081 atagtcctga tgaagaacac aatatttcat taatagaaga tcgcggtttt gtattcaata
     1141 ctgttgatat taaagatggt gagttaaaag aagttaaagc gcacaatcag cgtcaagcaa
     1201 gaatgcgcaa agatgaccat ttaactaatc aagtgaagaa caaagttcga agtaaaatta
     1261 aaaacaaagt taaaccaggt tataagaaga aatttaaaca agaagttgaa aaaatgaaac
     1321 gtcaagagcg taagcaattt agtaagcagc aaaatagaca aaaacgtaag caaaacaaaa
     1381 aaggttaggt gtgttttatg ttattaggat cacatgtttc aatgagtggt aaaaagatgt
     1441 tagaaggttc tgctatagaa gcgcatgaat atggtgaaac aacatttatg atttatactg
     1501 gtgcacctca aaacacgcgc cgtaaaagta ttgaagattt aaatataact aaaggtcatg
     1561 aagtgatgga aaaatatggt ttatctaata ttgttgttca tgcaccatac atcattaata
     1621 ttgcaaatac aaccaaacca gaaacgttta atcttggtgt agatttcttg caacaagaaa
     1681 ttgaaagaac gcaagctata ggggctaaag atattgtatt acatccaggt gcgcatgttg
     1741 gagctggtgt tgatgccgga attaataaga ttattgaagg attaaatgaa gttttaacga
     1801 atgacaataa tgtacgtatt gcgcttgaaa cgatggcagg taaaggtaca gagattggtc
     1861 gctcatttga agagttagca cgtattattg atggagttca taataacgaa cgtttatcag
     1921 tatgttttga tacatgtcat acgcatgatg ctggatataa tgttaaagag gactttgatg
     1981 gcgtattaaa tgaatttgat aaaatcattg gagtcgacag aatcaaagtt gttcatgtca
     2041 atgattctaa aaatgaccgt ggcgctcaaa aagaccgaca tgaaaatatt ggcttcggtt
     2101 acattggttt tgatgcatta aattacattg tgcatcatga tagttttaaa gatattccga
     2161 aaatcttaga aacaccttat gttggtgaag ataagaaaaa taaaaaaccg ccatataaac
     2221 ttgaaattga aatgttaaaa caacagcaat ttgacccaga attaaaaaat aaggttatgc
     2281 aacaataaaa tgataatgct ctcttcaaga aatgcgataa ataaaacttg aagggggcat
     2341 atctctttat tttgtctaat tttgagtcgt aaacattact gtttacatat gttactagga
     2401 gttgtatagt tgaatgcgag gtgagaatac gatgacacca gtctttgaat tgaaaaatgt
     2461 caattactac tatgatcata aaaaagtgtt agaaaatata aacattaaaa taaataaagg
     2521 tgaattttta gcaattgttg gaccaaatgg tgctggtaaa tcaacattat tgaagttgat
     2581 tctagggtta ttacctttac aaagtggtga gatttttgtt gaaggtattg attttaaaaa
     2641 taagaaaaca tcaattaaat taagctatgt atcacaaaaa gcaaatgcct ttaattcagg
     2701 tttcccagca agtgttaaag aagttgtttt aagcggatta acaaagacaa aacgtctttt
     2761 ccaaacattt aatagcaaag ataatgaaaa agtgattaaa gtactagaaa gactgaatat
     2821 aagtgattta attcataaaa atatagcaga attatcaggt ggtcaacaac aacgtgtaat
     2881 gattgctcga gcattgattt cagaacctgc agtattagta cttgatgaac caacgaatgg
     2941 tattgatgca aaacatgtaa gtgaatttta taatacttta gatcaattaa aacaagaagg
     3001 tatcaccatt atcttagtta ctcatgatat cggtgttgta gcagatactg ctactgaagt
     3061 agcatgttta aataagcatt tgcatttcca tggtacaact gatgagttta aatcacttga
     3121 tgaagttgaa atttcaaaaa tttatggaca tcctgtacgt tttgtcgatc atcagcataa
     3181 tcgagaatgt tgtaattaat aaagatttga gtttatttaa aatggaaagg cgtgtcacaa
     3241 ttgatagatg cattattgaa ttttgatttt atgaggtact ctttaattag tggtatcttg
     3301 ataggtttta ttgcgcctct aatcggtgct tttatcgttg ttagacgact atcacttata
     3361 gctgatgctc taagtcatgt aactttaggt ggtatatctt tcggtatgtt tttacttact
     3421 attatgccaa cactagtatt tattaatcca atgtggtttg gaatcttatt cgcaatagta
     3481 ggtgcgcttc taattgaaaa attaagaacg tcatacactg cttaccaaga aattgctatt
     3541 ccaattataa tgagtgctgg tatcgccttg agtgcaatct tcatttcatt agctgatggg
     3601 ttcaatcaag aaattgttgg attgctattt ggctcaataa gcgcagtgaa tataagtgat
     3661 ttaacaacga ttattgtcat tgccatcata gttgttctgt ttattacatt gttttataaa
     3721 gaattattta tattgtcatt tgatgaggaa tatagtaaag tcattggcat tcctaaatgg
     3781 attcaatttt tatttattgt tattgtagcg atggttattt ctgcctcaat gcgtgttgta
     3841 gggatactgt tagtaagtgc gttaatcacg ttaccaattg caataagtat gcgaataaca
     3901 aaaagtttta agcaattgat attactaagt gtatttttag gtgaattatc agtaatatta
     3961 ggcttagtcc tagctttcta tatggacatt tctccgggtg gtgtaattgt agttctactt
     4021 gttattttac ttatgattac aatggcttat cagaaaatgc gaatgaagtt taaaaaggga
     4081 gctaatatca atgaatacaa atgatgctat taaaatttta aaagagaacg gtttaaaata
     4141 tacagataaa cgtaaagata tgttagatat ttttgtcgaa gaagataagt atataaacgc
     4201 aaagtatata caacaagtta tggatgaaaa ttatcctgga atttcattcg acacaatata
     4261 tagaaacctg cacttattta aagatttagg aattattgaa aatacagaac ttgatggtga
     4321 aatgaagttt agaatcgctt gtacaaacca tcatcatcat cattttatct gtgaaaagtg
     4381 tggagataca aaggtaatag attattgtcc aatagatcag ataaaattat cactacctgg
     4441 tgttaatatt cacaaacaca aacttgaagt ttatggtgta tgtgagtctt gccaagatta
     4501 atataaagaa atgagat
//
LOCUS       DAKTGS010000172         4446 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.177, whole genome shotgun sequence.
ACCESSION   DAKTGS010000172 DAKTGS010000000
VERSION     DAKTGS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.177"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(120..1475)
                     /locus_tag="O0286_002603"
     CDS             complement(120..1475)
                     /locus_tag="O0286_002603"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCY0170937.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(1502..2434)
                     /locus_tag="O0286_002604"
     CDS             complement(1502..2434)
                     /locus_tag="O0286_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCY0170938.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(2436..3245)
                     /locus_tag="O0286_002605"
     CDS             complement(2436..3245)
                     /locus_tag="O0286_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCY0170939.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(3434..4342)
                     /gene="rocF"
                     /locus_tag="O0286_002606"
     CDS             complement(3434..4342)
                     /gene="rocF"
                     /locus_tag="O0286_002606"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCY0170940.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
BASE COUNT         1376 a          873 c          552 g         1645 t
ORIGIN      
        1 attacatatt gattactttg tttttatttt gtttatatca ttttacgttt gtactataaa
       61 ttatttctac aaacacaaaa aaccgatgca tacgcatcgg ctcatttgta atacagtatt
      121 tatttatcta atcccatttt atcttgaacc acatcagcta tttgttgtgc aaatctttca
      181 gcatcttcat cagttgctgc ttcaaccatg acacgaacta atggttctgt tccagaaggt
      241 cttactaaaa ttcgaccttc tccattcatt tctacttcta ctttagtcat aacttcttta
      301 acgtcaacat tttcttcaac acgatattta tctgttacgc gtacgttaat taatgattgt
      361 ggatattttt tcatttgtcc agctaattca cttagtgatt taccagtcat ttttattaca
      421 gaagctaatt gaataccagt taataaacca tcaccagttg tattgtaatc catcataacg
      481 atatgtccag attgttctcc acctaagtta taattaccgc gacgcatttc ttctactaca
      541 tatctgtcgc caactttagt tttattagat ttaattcctt cttgttcaag cgctttgtaa
      601 aaacctaaat tactcataac agtagaaaca atcatgtcat tattcaattc ttgattttta
      661 tgcatttctt gaccaataat aaacataatt tggtcaccgt caacgatttg accattctca
      721 tctactgcta tgattctgtc tccatcgccg tcaaatgcta acccaaaatc actttcagtt
      781 tcaactactt tttcagctaa tttttcagga tgtgtagagc cacatttctc attgatatta
      841 tatccatcag gactacatcc aattgtttca gtatctgctt ctaagtcacc aaataagaat
      901 ggcgctagtg atgatgttga accatttgca ccatctaaag caattttcaa accttcaaag
      961 ttaacatcta ctgttgattt taaatagctc aaatattttt gtgccccttc aaagtaatct
     1021 gaataatgta caatatcatt gccaactggt cttggtaatt ctgggttttc ttgatccaat
     1081 aatgcttcaa tttcattttc ttgttcatct gatagtttaa aaccatctga tccaaagaat
     1141 ttaataccat tatctgcaac tggattatga gaggctgaaa tcattacacc taactctgca
     1201 cccatatcgc gtgttaaata tgcaacacct ggtgttgaaa taatacctaa tcgcatcact
     1261 tctgcaccaa ttgaaatcaa accagctatt aatgctgatt ctaacatttc acctgaaact
     1321 ctagtatcgc gacctacaag tacacgtggg tgtttttcac ctttattatg tgctagaaca
     1381 tagccaccgt atcttcctaa tttaaatgcc aattcaggtg ttagttcttg gtttgcgaca
     1441 cctcttactc cgtctgtacc aaaatatttt cccattgttt actctccttt aatttagcta
     1501 tttattttac atttatataa gccttcgttt cacttggttg tgctttagtg acatgttctg
     1561 gtaaattgat ttttacagtc ttttcagttg attctgaaat accatctaaa tctacttctg
     1621 catcaacttc gcttatattt tgtaagtcat ctcgactacc gaagatttca atttctttat
     1681 cttctaaatc aatcgaactt aactctttat catctgctaa actacctttc tgtttaacat
     1741 ttactttaac ctttttgcta aaaggctcta ctttaacttg taaattcact tcattaggtt
     1801 gaatcgatac atttaattta ttcagttttt tatcaaaagc cgttacttct gcgacatctt
     1861 ttgtgtcacc attaatcttt ttattagttt taaaagtggc ttttaaataa gcgatatcat
     1921 tcaattgttc ttctccacct gttactttaa ctgtttgtgg tgaaacttct tgctttgtaa
     1981 ttttataaag tggatcaata tcactctgac ttacatcagg ttgaactgtc atctttttag
     2041 ttactttgtt ttcaagcgta atatttgcta atttaggttt tacagaataa tgaatgtcat
     2101 cagctaaccc tttaacttga tacttctctt gatattttcc agctttagca tttgataaat
     2161 caatcactac tcttaaatct tctggatttt caatttttat tatctttgat tgtggtcctg
     2221 aaatagtcac attaactgtt tcaggcgctt ttgttaaatg caaatcttta gtgttataaa
     2281 gaatttcaac gggtacatct tgaatcgttt tactagactt ttgaccaaga ttaccagtgt
     2341 taaagatatt tccaaaaaca ttgttaacag ataaaaagaa aaacaatgcc aaaagaaagg
     2401 caataaatct caagccccat ttactttcta gcatattatt tcacaccttt cttttgaaag
     2461 cgtgtgccaa accaatgttc agcaagcaac tcttcaaaaa tttcgtttga aatgtctcgt
     2521 cgtaattttc catcaaatgt taccgaaata tcaccagttt cttcagatac aataacggta
     2581 aatgcatcag atacttctga aataccaacc gcagctctat gtcttgtacc caaactttta
     2641 gatatcttag gactatcaga caatggcaaa taacttgctg ctgctgcaat cttcgtgcct
     2701 tgaataatca ttgcaccatc atgtaaaggt gtgttaggta taaagacatt aattaaaagt
     2761 tcttgcgaaa tatttgaatc cattgcaata cctgtttcaa tataatcttg aagacctgtt
     2821 tctttttcaa agacaattaa tgcacctata cgtcttttag ccatatattg cacagccttt
     2881 gaaaccgatt gaatcaattt ctcttcatct ttactatacg tattagaagt atagcgtttt
     2941 aaaaagctac ctctaccaag ttgttctaac gcacgtctaa tttctggttg gaatattact
     3001 attaaagcta atacccccca ttgaataacg atatcgaata atttagatgt tgcagtcaag
     3061 ttcaatatca tacttatctg ctgaccaata acaattacta atatcccttt aagtaattgt
     3121 atcgctttag ttcccttaaa gaccgtgatg agaagataaa gtacatacca aactatcagt
     3181 aaatcaagga tactcgttac aatttttaac gtactgaggt tttgaaaaaa gttggaaaaa
     3241 tccataacat ctcctccggg taatattttt ccataatacc cattatacca atcatttaca
     3301 tcactgtcat acgaacatat atttaaatag aaaaaattta ttttaaagat tataactact
     3361 cttaatcatt ttagtgaatt aaaaaaagta gtgcaaaaag caaaatatac tttatacact
     3421 acaaatcatt tatttataat aaagtttcac caaaaaatgt tccaactaat gaaaccgctt
     3481 gttcagcagt atgattatta ctgtcaatca atggatttac ttcaactaaa tccattgagg
     3541 aaattaaatg tgattgatgc agtaattcca atgcaaaatg gctttctcta taactaagac
     3601 cacccaaaac tctagtacca gtgcctggcg tttcaagcgg atctaaagca tcaacatcta
     3661 aagataaatg aacgccatca acattgcgtg acttcaaata ttctattgta ttttcaatta
     3721 cttcctttat ccccaattta tcaatatctg acatagtaaa tgttttaata ttatgatctt
     3781 tgataaattg tctttcacct ttatctaaat ctctcatacc aattagtacg atgttttctg
     3841 gcttgattac attactattt aattctaaaa gttctttggg accttcgcct gtcaaaatcc
     3901 ttagaggcat accatgaata tttccacttg gtgactcttc aggtatattt aaatcaccat
     3961 gtgcatcata ccaaataaca cctaaattat tataatgttt acttattgct gatactgaac
     4021 ctaccgcaat agaatgatct ccaccaagaa ctagaggaaa tctgttattt tcaattgatg
     4081 ctgaaacctc tttatttaat ttttgattaa catctataat ttcatcataa tttcttaatc
     4141 ctttttgttc actatgaaat ttttcaatgt tcacagcagg taccttaata tcccccttgt
     4201 catatacatc aaggtctaat tgctttaatc ttgaaattaa tccagcatat ctaattgctg
     4261 ttggtcctaa atcaacacct aattttcttt gtccaaatgt tgatggtgca cctataatat
     4321 caattgcttt tgtctttgtc ataagcgtcc ccctttgctc tatgtaatta aagaataatg
     4381 tatgcgctta ccattatcaa gcaatagcta cacatataat ctgtttatct tattacttca
     4441 taaaaa
//
LOCUS       DAKTGS010000173         4439 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.178, whole genome shotgun sequence.
ACCESSION   DAKTGS010000173 DAKTGS010000000
VERSION     DAKTGS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.178"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..979
                     /locus_tag="O0286_002607"
     CDS             <1..979
                     /locus_tag="O0286_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170941.1"
                     /translation="KKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMIT
                     IVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIF
                     IKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATI
                     GNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTT
                     FMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSL
                     QLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSKKQFE"
     gene            1165..2406
                     /locus_tag="O0286_002608"
     CDS             1165..2406
                     /locus_tag="O0286_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCY0170942.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLN
                     YDLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQ
                     FFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALT
                     AFQDRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIK
                     QKNDKLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQK
                     QIKAAQDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKP
                     LKENIEIALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELY
                     SEQEQQQEQQKNQKRDRGMHL"
     gene            2933..3877
                     /locus_tag="O0286_002609"
     CDS             2933..3877
                     /locus_tag="O0286_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCY0170943.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLS
                     FDAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADS
                     WDRRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDK
                     AVKKTIFYGRNGKPETKYFGVRDSNRFIRIYNKKKERKDNADAEVMSEHLWRVEIELK
                     RDMVDYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLI
                     KEISPVDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     gene            4039..>4439
                     /locus_tag="O0286_002610"
     CDS             4039..>4439
                     /locus_tag="O0286_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0170944.1"
                     /translation="MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHF
                     NTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNH
                     FFILIFGRLVQGVGSAAFPSLIMVVVARNIT"
BASE COUNT         1651 a          541 c          791 g         1456 t
ORIGIN      
        1 aaaaaaacaa ggcaaagcct ttggttttat aggatcaatt gtagctttag gtgaagggtt
       61 aggtccttca atagggggaa taatagcaca ttatattcat tggtcttacc tacttatact
      121 tcctatgatt acaatagtaa ctataccttt tcttattaaa gtaatggtac ctggtaaatc
      181 aacaaaaaat acattagata tcgtaggtat tgttttaatg tctataagta ttatatgttt
      241 tatgttattt acgacaaatt ataattggac ttttttaata ctcttcacaa tcttttttgt
      301 gatttttatt aaacatattt caagagtttc taaccctttt attaatccta aactagggaa
      361 aaacattccg tttatgcttg gtttgttttc tggtgggcta atattttcta tagtagctgg
      421 ttttatatca atggtgcctt atatgatgaa aactatttat catgtaaatg tagcgacaat
      481 aggtaatagt gttatttttc ctggaaccat gagtgttatt gtttttggtt attttggtgg
      541 ttttttagtg gatagaaaag gatcattatt tgtttttatt ttaggatcat tgtctatctc
      601 tataagtttt ttaactattg cattttttgt tgagtttagt atgtggttga ctacttttat
      661 gtttatattt gttatgggcg gattatcttt tactaaaaca gttatatcaa aaatagtatc
      721 aagtagtctt tctgaagaag aagttgcttc tggaatgagt ttgctaaatt tcacaagttt
      781 tttatcagag ggaacaggta tagcaattgt aggaggttta ttgtcactac aattgattaa
      841 tcgtaaacta gttctggaat ttataaatta ttcttctgga gtgtatagta atattcttgt
      901 agccatggct atccttatta ttttatgttg tcttttgacg attattgtat ttaaacgttc
      961 taaaaagcag tttgaatagt tatattatat tttggtttag aactatgagt ggctagcatt
     1021 ttgccactca ttttttgcgt tagcaaaaac aggtttaagc ctcgcagagc acacgtatta
     1081 acgacttatt aaaaataagt ctagtgtgtt agacttaaac tattaaatac acatgaaacc
     1141 tttgtgctta ggagtgattt ttatatgtct tattccattg ttagagtttc aaaagttaaa
     1201 tctggaacaa atacaacggg catacaaaaa catgttcaaa gagaaaataa taattatgaa
     1261 aatgaagata tagaccatag taaaacttac ttaaattatg atttggtaaa tgctaataaa
     1321 cagaatttta ataacttgat tgatgaaaaa atcgaacaga attatacagg caaaagaaaa
     1381 attagaacag acgcgattaa acacattgat ggtttaatta catcagacaa tgatttcttt
     1441 gataatcaaa cgccagaaga tacaaagcag ttttttgaat atgctaaaga gtttttagaa
     1501 caagaatacg gtaaagataa tttattatat gcaacagttc acatggacga aaaaacacca
     1561 catatgcatt atggcgttgt tccaataact gatgatggtc gtttaagtgc taaagaagtt
     1621 gtaggtaata aaaaagcttt aacagcgttt caagatagat ttaatgagca tgttaaacaa
     1681 cgaggatatg atttagaacg tgggcaatca agacaagtaa caaatgctaa acatgagcaa
     1741 ataagtcagt ataaacaaaa aacagaatat cataagcaag aatatgaacg tgagagccaa
     1801 aaaacagacc atataaagca aaagaacgat aaattaatgc aagagtacca aaaatcgtta
     1861 aatacgctta aaaagcctat aaatgttccg tatgagcaag aaactgaaaa agtaggtggt
     1921 ttatttagca aagaaataca agaaactgga aatgttgtaa taagccaaaa agatttcaat
     1981 gaatttcaga aacagataaa agctgctcaa gatatttcgg aagattacga gtatataaag
     2041 tctggtagag ccttagatga taaagataag gaaatacgag agaaagatga tttattaaat
     2101 aaagcagttg agcgtattga aaacgcagac gataatttta accaacttta cgaaaatgca
     2161 aagccactta aagagaatat agaaatagcg ttaaagcttt taaaaatctt actaaaagag
     2221 ttagaacgag ttttaggaag aaataccttt gcggaaagag ttaataagtt aacagaagat
     2281 gaaccaaaac taaatggttt agcaggaaac ttagataaaa aaatgaatcc agaattatat
     2341 tcagaacagg aacagcaaca agaacaacaa aagaatcaaa aacgagatag aggtatgcac
     2401 ttatagaaca tgcatttatg ccgagaaaac ttattggttg gaatgggcta tgtgttagct
     2461 aacttgttag cgagttggtt ggacttgaat tgggattaat cccaagaaag taccaactca
     2521 acaacacata aagccctgta ggttccgacc aataaggaaa ttggaataaa gcaataaaag
     2581 gagttgaaga aatgaaattc agagaagcct ttgagaattt tataacaagt aagtatgtac
     2641 ttggtgtttt agtagtttta actgtttacc agataataca aatgcttaaa taaaaaaaga
     2701 cttgatctga ttagaccaag tcttttgata gtgttatatt aataacaaaa taaaaaggag
     2761 tcgctcacgc cctgaccaaa gtttgtgaac gacatcattc aaagaaaaaa acactgagtt
     2821 atttttataa tcttgtatat ttagatatta aacgatattt aaatatacat caagatatat
     2881 atttgggtga gcgattcctt aaacgaaatt gagattaagg agtcgatttt ttatgtataa
     2941 aaacaatcat gcaaatcatt caaatcattt ggaaaatcac gatttagaca atttttctaa
     3001 aaccggctac tctaatagcc ggttggacgc acatactgtg tgcatatctg atccaaaatt
     3061 aagttttgat gcaatgacga tcgttggaaa tctcaaccga gacaacgctc aagccctttc
     3121 taaatttatg agtgtagagc cccaaataag actttgggat attcttcaaa caaagtttaa
     3181 agctaaagca cttcaagaaa aagtttatat tgaatatgac aaagtgaaag cagatagttg
     3241 ggatagacgt aatatgcgta ttgaatttaa tccaaacaaa cttacacgag atgaaatgat
     3301 ttggttaaaa caaaatataa taagctacat ggaagatgac ggttttacaa gattagattt
     3361 agcctttgat tttgaagatg atttgagtga ctactatgca atgtctgata aagcagttaa
     3421 gaaaactatt ttttatggtc gtaatggtaa gccagaaaca aaatattttg gcgtgagaga
     3481 tagtaataga tttattagaa tttataataa aaagaaagaa cgtaaagata atgcagatgc
     3541 tgaagttatg tctgaacatt tatggcgtgt agaaatcgaa cttaaaagag atatggtgga
     3601 ttactggaat gattgcttta gtgatttaca tatcttgcaa ccagattgga aaactatcca
     3661 acgcactgcg gatagagcaa tagtttttat gttattgagt gatgaagaag aatggggaaa
     3721 gcttcacaga aattctagaa caaaatataa gaatttgata aaagaaattt cgccagtcga
     3781 tttaacggac ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaaacaaat
     3841 cgatttttgg caacatgaat ttaaattttg gaaatagtgt acatattaat attactgaac
     3901 aaaaatgata tatttaaact attctaattt aggaggattt ttttatgaag tgtctattta
     3961 aaaatttggg gaatttatat gaggtgaaag aataatttac ccctataaac tttagtcacc
     4021 tcaagtaaag aggtaaaatt gtttagttta tataaaaaat ttaaaggttt gttttatagc
     4081 gttttatttt ggctttgtat tctttcattt tttagtgtat taaatgaaat ggttttaaat
     4141 gtttctttac ctgatattgc aaatcatttt aatactactc ctggaattac aaactgggta
     4201 aacactgcat atatgttaac tttttcgata ggaacagcag tatatggaaa attatctgat
     4261 tatataaata taaaaaaatt gttaattatt ggtattagtt tgagctgtct tggttcattg
     4321 attgctttta ttggtcacaa tcactttttt attttgattt ttggtaggtt agtacaagga
     4381 gtaggatctg ctgcattccc ttcactgatt atggtggttg tagctagaaa tattacaag
//
LOCUS       DAKTGS010000174         4318 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.179, whole genome shotgun sequence.
ACCESSION   DAKTGS010000174 DAKTGS010000000
VERSION     DAKTGS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.179"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            643..1578
                     /gene="lukE"
                     /locus_tag="O0286_002611"
     CDS             643..1578
                     /gene="lukE"
                     /locus_tag="O0286_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCY0170945.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1580..2563
                     /gene="lukD"
                     /locus_tag="O0286_002612"
     CDS             1580..2563
                     /gene="lukD"
                     /locus_tag="O0286_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCY0170946.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2805..2948
                     /locus_tag="O0286_002613"
     CDS             2805..2948
                     /locus_tag="O0286_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0170947.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
     gene            3685..3828
                     /locus_tag="O0286_002614"
     CDS             3685..3828
                     /locus_tag="O0286_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0170948.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            3893..>4318
                     /locus_tag="O0286_002615"
     CDS             3893..>4318
                     /locus_tag="O0286_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCY0170949.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQN"
BASE COUNT         1663 a          541 c          706 g         1408 t
ORIGIN      
        1 atgcgtggac agtaaaacga agtacatgcg aatgtagtag ccgtttacta tgagactagg
       61 cattataatt gaatatcata gtataaattg ccatgagagt agaagcttca gtttatgaag
      121 acttcgaaat catggttttt tatttttaaa gtgaaatttt agttcatagt aaatatatat
      181 atgtttttaa ttagactaaa tcatagcatt aaattttttt agatgaagta agcgatatta
      241 cgaaatgata tatagaacat ttcagttata acagtatacg aagtaatcat cttcgtttaa
      301 cggacaatag ttaataatca gttaatttta aattcaatta tagattattt gtgataatac
      361 aaacattatt tttgaagatt tgattaaagt cgtttttatt tttacagaga aaagattgag
      421 tatcggtaat tttatcatat tgttcgaata taaagggaac tatttaaagg taatgaaggt
      481 tgcgccaaaa tgaccggttt tcagcagcat aaatgaataa attgtgtcat ttttaatatt
      541 ttatagcatt ttatttttta atgaacagtt aattttataa ataagtgaaa taatctagaa
      601 taaatttgta ttaaaaattt atatagaaag aaagtgaaac ttatgtttaa gaaaaaaatg
      661 ttagctgcaa ctttgtcagt aggactgatt gcacctttag catctccgat tcaagaatct
      721 agagcaaata ctaatattga aaatattggt gatggtgctg aagtaatcaa acgtacggag
      781 gatgtaagta gtaagaaatg gggcgttact caaaatgtcc aattcgactt tgtaaaagat
      841 aaaaaatata acaaagacgc tttaattgtt aaaatgcaag gttttattaa ttccagaact
      901 tcattttcag atgtgaaggg tagtggatat gaattaacta aacgaatgat ttggccattc
      961 caatataata taggactgac gactaaagat ccaaatgtta gcttaatcaa ttaccttcct
     1021 aaaaacaaaa tagaaactac tgatgttggt caaacattag gatataacat tggaggtaat
     1081 ttccagtcag caccatctat aggtggcaat ggctcattta attattctaa aacaattagt
     1141 tatacccaaa agagttatgt cagtgaagta gacaagcaaa actcaaaatc tgttaaatgg
     1201 ggtgttaaag caaacgaatt tgttacgcct gatggaaaaa aatctgcgca tgatagatat
     1261 ttattcgtac aaagtccaaa tggtccaaca ggttcagcaa gagaatattt tgctcctgat
     1321 aatcaattgc cacctttagt tcaaagtggc tttaatccat cgtttatcac tacactatca
     1381 catgaaaaag gttcaagtga tacgagtgaa tttgaaattt catatggtag aaacttagat
     1441 attacatatg cgactttatt ccctagaact ggtatttacg cagaaagaaa gcataatgca
     1501 tttgtaaata gaaactttgt agttagatat gaagttaatt ggaaaacaca cgaaattaaa
     1561 gtgaaaggac ataattaata tgaaaatgaa aaaattagtc aaatcatcag ttgcttcatc
     1621 aattgcactg cttttgctat cgaatacagt tgatgcagct caacatatca cacctgtaag
     1681 cgagaaaaaa gtagatgaca aaatcacttt atacaaaaca acagcaacat ctgataatga
     1741 taaattgaat atttctcaaa ttttaacgtt taatttcatt aaggataaaa gttatgacaa
     1801 agatacgtta gtacttaagg cagccggaaa cattaattca ggttataaaa agcctaatcc
     1861 aaaagattac aattactcac agttttattg gggcggtaag tataatgttt cggttagttc
     1921 agaatcaaat gatgctgtaa atgttgttga ctatgcacct aaaaatcaaa atgaagaatt
     1981 ccaagttcaa caaacattag gttattctta tggcggagat attaatatat ctaatggctt
     2041 atcaggtgga ttaaatggat caaaatcatt ttcagaaacg ataaattata aacaagaaag
     2101 ttacagaact acgattgata gaaaaacaaa tcataaatca attggctggg gtgttgaggc
     2161 gcacaaaatt atgaataatg gttggggacc atatggtaga gatagttatg acccaacata
     2221 tggtaatgaa ctgtttttag gcggtagaca aagtagttca aatgctggtc aaaatttctt
     2281 gccaacacat caaatgcctt tattggcgcg tggtaacttt aacccagaat ttataagcgt
     2341 actttctcat aaacaaaatg atacaaaaaa atctaaaatc aaagtaactt accaaagaga
     2401 aatggataga tatactaatc aatggaatcg actacactgg gttggtaata actacaaaaa
     2461 tcaaaataca gtaacgttta catctactta tgaagttgac tggcaaaacc atactgttaa
     2521 attaatcggt acggattcta aagaaactaa tcctggagta taactgttag aaatagagga
     2581 agttaaaccc gaaatttaag agttaaacaa aaaatattca caacatatta aaaaaagaag
     2641 ttacattatt tgtttaaact aacaatagta ccataaaatt aatgacgatt tacaaattaa
     2701 taacaaataa ttattattaa attcattgat ttattgtgtg gtaatgatta atatgacaat
     2761 tgaagtgggt cttaacttca acttttaaat agggggtttt atctttggaa aaagttcttg
     2821 atttagacgt gcaagttaaa ggaaataaca acactaatga ttcagcgggt gacgaaagaa
     2881 taactagcca tcttttttgt agctttggtt gtgaaaagac gggtagtttt aacagcttct
     2941 gttgttaagg atacagcaaa tagagcctga aagggctcta tttgtctttt taaatatatt
     3001 aagtgctaaa aaacggaaac agtttattaa tcgccaggta tttttataat tagccttcta
     3061 tattatcagt tgagtgtttc aataaattct gactttggtg atggaaagtt atacttttag
     3121 ttgtaatata gttttcatga cttcctgtta aataagttag gattatgtaa ttagcctcta
     3181 tctttacata taagtaatga caagctttgg ttattatatt gaccctctta gaacatgtca
     3241 tttaagaaaa taatcaagtc attgaaatta tatgtttata tcctcatctt taaaataact
     3301 tcaattatta tttgctaaat tttgtataaa tagaacaaaa ctgcataaat atctattaat
     3361 taatctcaag cagccatctc caaattcgta atagtattga tagataaaaa tatctaagta
     3421 tttgtataaa tgaattgatt aatctattaa aaaattcatc aataaattta aaattatgaa
     3481 cttagatgca aaattattag tgaaaataaa tagtaataaa aaaacacaaa aatctaatta
     3541 tcaaaatagt attactaaat taataagtgt ttacaaaaaa ttcacaaaat ataattatat
     3601 aatttattga tttgttgtgt gaaaagggat aatatgacaa ttgaagtagg ccttaacttc
     3661 aactaataat aggaggtttt atctatggaa aaagttcttg atttagacgt gcaagttaaa
     3721 gcaaacaata actcaaatga ttcagcaggt gacgaacgta ttacaagtca tagtttatgt
     3781 actcctggtt gtgctaagac tggtagtttt aatagcttct gctgttaaga ttacagcaaa
     3841 tagagcctga aagggctcta tttgtctatt caaatatgtg aggtgcttac aaatgaacac
     3901 tatttacgaa ccgtcaagta tttgtatgat tagaacacct ttattatcag ttgaattttt
     3961 caatttattt ttaaatactg aacaaattaa atatagcgac ttacaattaa atgctcagat
     4021 gaaagaatca atcctaacaa caacatttaa cttatattgt actttacaag aaataaattt
     4081 tgatggcgat aacaagaagg ttagagatgc taaagaaagt ttattaaaat atttaattag
     4141 aatgtcaaca cgtccaactc catttggatt attaagtggt attaatttgg ggcattttgt
     4201 aaatgaacca acacgtctta aagttggaaa tagtattcaa aaatatgtga aagtcgatgg
     4261 cgaatggttg tataaactgg ttagttatat tgagagtatt gatgaatatt atcaaaat
//
LOCUS       DAKTGS010000175         4077 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.180, whole genome shotgun sequence.
ACCESSION   DAKTGS010000175 DAKTGS010000000
VERSION     DAKTGS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.180"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..1511
                     /gene="vraG"
                     /locus_tag="O0286_002616"
     CDS             <1..1511
                     /gene="vraG"
                     /locus_tag="O0286_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCY0170950.1"
                     /translation="KLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLIGVAYFLTSAQN
                     FILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYLSLNIVQYYDSI
                     GTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSSIMYRIKKNAFS
                     LTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQLASELNNQKIP
                     HFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQADLFVAEGSIKD
                     LVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLNDKDYQEIRKYTK
                     AKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTGILLFVTSFLGI
                     TFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIMFNFGLPLVIAL
                     SHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRTIRHSI"
     gene            2236..2853
                     /locus_tag="O0286_002617"
     CDS             2236..2853
                     /locus_tag="O0286_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCY0170951.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            2869..3876
                     /locus_tag="O0286_002618"
     CDS             2869..3876
                     /locus_tag="O0286_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCY0170952.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
BASE COUNT         1447 a          524 c          703 g         1403 t
ORIGIN      
        1 cgaaactgtt attaatgatt gtctttacat tattaggaat taaagaaaag gttccaatta
       61 tttttagttt gagggcggta tttgaaacat taatgttaat cggtgtcgct tattttttaa
      121 catctgctca aaattttata ttagtgttca aacaatctat ttcacagatg tcaaagaata
      181 accaggttaa agaaacaaat cataataaaa ttacatttga agaggttgtt ttaggcatct
      241 taggtatagt attgattacc acaggatact atctatcttt gaacattgtt caatattatg
      301 attctatcgg tacacttatg tttattttat tgtcaactgt gattggggca tacttatttt
      361 ttaaaagctc tgtttctcta gtttttaaaa tggtgaagaa gtttagaaaa ggtgttataa
      421 gtgtaaatga tgtcatgttc tcatcatcta ttatgtatcg tattaagaaa aatgcttttt
      481 cacttacggt catggcaatc atttcagcga ttactgtttc agttctttgc tttgctgcta
      541 taagtagagc gtccttatca agtgaaataa aatatactgc accacacgac gttacaatta
      601 aagaccaaca aaaagctaat caattagcaa gtgaattaaa caatcaaaaa attcctcatt
      661 tttataatta taaagaagta attcatacga aattgtataa agataattta tttgatgtaa
      721 aagcgaaaga accatacaat gtaacaatta ctagtgataa atacatccct aatactgatt
      781 tgaaacgtgg gcaagctgat ttatttgtag cggaaggttc tatcaaagat ttagtgaaac
      841 ataagaagca tggtaaggca attataggaa cgaaaaaaca tcatgttaat attaagttac
      901 gtaaagatat taataaaatc tattttatga cagatgttga tttaggtgga ccaacgtttg
      961 tcttaaatga caaagactat caagaaataa gaaagtatac aaaggcaaag catatcgtct
     1021 ctcaatttgg attcgatttg aaacataaaa aagatgcttt agcattagaa aaagcgaaaa
     1081 ataaagttga taaatctatt gaaacaagaa gtgaagcgat aagctcaata tcaagtttaa
     1141 ccggaatatt attatttgta acatcatttt taggtattac attcttgatt gctgtatgtt
     1201 gcattatata cataaagcaa atagatgaaa ccgaagatga gttagagaat tatagtattt
     1261 tgagaaagct tggatttaca caaaaagata tggcaagggg actaaagttt aaaattatgt
     1321 ttaattttgg gttaccttta gttattgcac tatcacatgc atattttaca tcattagcat
     1381 atatgaaatt aatgggtaca acgaatcaaa taccggtttt catagtaatg ggattataca
     1441 tttgtatgta tgctgttttt gcagtgacgg cttataatca ttccaagcga acaattagac
     1501 attccatata aaatatacag atggctttca gtagagtagt ggattcggat tcacgaacta
     1561 tactggaagc tttttattat aaatgaagag aagttatatt tttagcatgt atagttgaat
     1621 actgggttaa aataccatat taataatgaa gtaaaggtat gagtgattat gaaagtgttt
     1681 tgaatgaaat atatttaatt ggtgatgctt ttaattgaaa agattaacag gattcaactt
     1741 tgtaaattgt attaaatgtg agaaaataaa agtatattca ttgagagata tatgagtcaa
     1801 tgatcgtttt aaacaagata agtgtatttt aatatgtaaa agttatgtaa taaatattgt
     1861 atcgttgcaa atttcccaac tatatccatt tacaattttt agagttgtat agataaaaaa
     1921 ttgcctatac aataactttt tcaaaacaaa tgcctactac tatttaaatt ttatgtaaaa
     1981 gttaagtttc ggaattgtta aaaatttgta aattgaattg tgggattgta aattttgtgc
     2041 tattttcata taagggcaaa tacagtaaat tgtttactgt gaggcatttt tgaaattaat
     2101 atcagtacac taaaattata ctgacgattg ataataacaa atttgtatca ttagtttgta
     2161 aattcacttg tcttaatttg aaacaaatta taactgaatg tgattggtga caatcgctta
     2221 aatggaggat tttaaatgtt tagtaagaaa aaagataagt ttatggttca attagaagag
     2281 atggttttca atctggatcg tgctgctatt gaattcggta aaatggattt caatacacat
     2341 ttagatttaa aagcatactc agacaacatt aaaacttatg agtcacatgg tgacgaatta
     2401 gtacatcaag taattactga tttaaatcaa acatttatca caccaattga acgtgaagat
     2461 attttatcat tatgtgatgc aattgatgat gttttagatg caattgaaga aacggcagct
     2521 atgtttgaaa tgtattcaat cgaatacaca gatgaatata tggctgagtt tgttgataac
     2581 attcaaaaag cagttgcaga aatgaaactt gctgtcggct tattagtcga taaaaaatta
     2641 tcacatatgc gtattcattc aattaatatt aaagaatttg aaacaaactg tgatggtatt
     2701 ttaagacagt caattaaaca tattttcaat agcgaaacag atccaatcac tttaattaaa
     2761 ataaaagata tttatgaaag catggaagaa atcgctgata aatgtcaaat cgtagcaaat
     2821 aattttgaaa ctattattat gaaaaatagc taaggggagt atatatttat gtcatatata
     2881 atcatcgtca ctatagctgt agttattttc tcgctgatat ttgactttat caatggattc
     2941 catgatacag ccaatgcagt agctactgct gtatctacta gagcgttaac gcctaaaacg
     3001 gcaattttaa tggcagcagt gatgaacttt ataggtgctt taacatttac gggcgttgca
     3061 ggcaccatta ctaaagacat tgtcgatcca tttaaattgg aaaatggatt agttgttgtg
     3121 ttagctgcaa tacttgcggc tattatttgg aatttagcta cttggtttta cggaattcca
     3181 agttcgtctt cacatgcact tataggttca attgcgggtg cagcaatcgc atctgaaggc
     3241 tcatttggag tgttacatta ccaaggtttc acaaaaatta ttattgtatt aatcgtttca
     3301 ccgattatcg cattttgtgt tggtttcttg atgtattcaa tttttaaagt tatctttaaa
     3361 aatgcaaatt taacaagagc gaatcgtaac tttagatttt tccaaatttt cacagcagcg
     3421 ttacaatcat tctctcacgg tacgaatgat gcgcaaaaat caatgggtat tattacgttg
     3481 gcattgattg tcgctaatgt acagaatgat ggcagtgttg aaccacagtt atgggtaaaa
     3541 tttgcctgtg cgacagcaat ggggcttggt actgcaattg gtggctggaa aattatcaaa
     3601 actgtaggtg gtaatattat gaaaatacgt ccagcaaatg gtgctgcggc cgatttatca
     3661 tctgcattaa caatttttgt tgcatcatcg ctacatttcc cattatcaac aactcacgtt
     3721 gtgtcatcat caatcttagg tgttggtgct tctaaccgag ctaaaggtgt aaaatggagc
     3781 actgcgcaac gaatgatcat tacatgggtg attacattac ctatttcagc attgttagca
     3841 ggtttactat tctatatact taacttattt ttctaattga aaataaaact aaactgaact
     3901 tcagtatcac aaacatatgg tggtattgaa gttcagtttt ttatgtaagt agataacata
     3961 ttttatagaa tttacgtaga atgaccaaga caaagcattt taattaggag gctatattat
     4021 aaatgtattc catggaacat aatcagttag gtcattaaaa tatatgtaag agcaaaa
//
LOCUS       DAKTGS010000176         4005 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.181, whole genome shotgun sequence.
ACCESSION   DAKTGS010000176 DAKTGS010000000
VERSION     DAKTGS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.181"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..212)
                     /locus_tag="O0286_002619"
     CDS             complement(<1..212)
                     /locus_tag="O0286_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCY0170953.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTL"
     gene            639..2126
                     /locus_tag="O0286_002620"
     CDS             639..2126
                     /locus_tag="O0286_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0170954.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            2772..3731
                     /locus_tag="O0286_002621"
     CDS             2772..3731
                     /locus_tag="O0286_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0170955.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(3793..3996)
                     /locus_tag="O0286_002622"
     CDS             complement(3793..3996)
                     /locus_tag="O0286_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170956.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
BASE COUNT         1391 a          565 c          741 g         1308 t
ORIGIN      
        1 tgtagcgtgt agcctcgatg taaataaaag ttttctacat attctttata aattttttga
       61 ttaataagcc aattgtaaaa gcgatctgaa cttcgagcaa aacaaaaaac tgctacgagt
      121 aaaaaagggg tcgttggcag taaaggtaat acggcacctg caataccaag cgctgtaaat
      181 attaagccaa tgacgattaa aataagtcgc attgaaaaaa ctccattcta gtactaatgc
      241 gcatgtaata ttgttttagt aatataactc atgctaaata taatgtgtat gataagtgca
      301 atgactcagt aaaatgaaac gatgttgaat tatccttgtc acattaacgc attttaagcg
      361 cgactttcat aacaaccaaa ctatttaatg agaattattc tcaagtatta tagttatatt
      421 atgtgtttta tttttgaaaa gtgcaatatg ttttcgaaaa taagattatt tttatgtgca
      481 aaaacgacgc aaaagtttta aaaatgagac ttctgtgagc tgattatttt ataaaatgta
      541 aacgcttact atataatgtg aatcatatcg tttaaaagca ttattaaata tgatgctaag
      601 agatttatat tatagccaat aaacaaagga gagataatat ggcagtaaac gttcgagatt
      661 atattgcaga gaattatggt ttatttatca atggggaatt tgttaaaggt agcagtgacg
      721 aaacaatcga agtgactaat ccagcaactg gagaaacact atcacatatt acaagagcaa
      781 aagataaaga tgtcgatcat gcagtcaaag tggcgcaaga ggcatttgaa tcatggtcat
      841 taacttctaa atcagaacgt gcacaaatgt tgcgtgatat tggtgataaa ttaatggcac
      901 aaaaagataa aattgcaatg attgaaacat taaataatgg taaaccgatt cgtgagacaa
      961 cagcaattga tattccattt gctgcaagac atttccatta tttcgcaagt gttattgaaa
     1021 cagaagaagg tacagtgaat gatatcgata aagacacaat gagtatcgta cgacatgagc
     1081 cgattggcgt cgtaggtgct gttgttgctt ggaacttccc aatgctatta gctgcatgga
     1141 agattgcgcc agccattgct gcaggtaata caattgtgat tcaaccttcg tcttcaacac
     1201 cattaagttt attggaagtt gctaaaattt tccaagaggt attacctaaa ggtgttgtca
     1261 atatactaac gggtaaaggt tcagaatcag gtaatgcaat tttcaatcat gatggtgtag
     1321 ataaattatc atttacgggc tcaactgatg taggttatca agttgccgaa gctgcagcaa
     1381 aacatctagt acccgctaca ttagagcttg gtggtaaaag cgccaatatc atattagatg
     1441 atgctaattt agaccttgca gttgaaggta ttcagttagg tattttattc aaccaaggtg
     1501 aagtatgtag tgcaggttct cgattattag ttcatgaaaa aatttatgat caattggtgc
     1561 cacgtttaca agaggcattt tcaaatatta aagttggaaa tccacaagat gaagctacac
     1621 aaatgggtag tcaaactggt aaggatcaat tagataaaat tcaatcatat attgatgcag
     1681 caaaagaatc agatgcacaa attttagcag gcggtcatcg cttaactgaa aatggattag
     1741 ataaagggtt cttctttgag ccgacattaa ttgctgtgcc agacaatcat cacaaattag
     1801 cacaagaaga aatatttgga ccagtgttaa cagtgattaa agtgaaggac gatcaagaag
     1861 caattgatat agctaatgat tctgagtatg gtttagcagg cggtgtattt tctcaaaata
     1921 tcacacgtgc attaaatatt gctaaagctg tacgtacagg acgtatttgg attaacactt
     1981 acaaccaagt accagaaggc gcaccatttg gtggttataa aaaatcaggt atcggtcgag
     2041 aaacttataa aggtgcgtta agtaactatc aacaagttaa aaatatttat attgatacaa
     2101 gcaatgcttt aaaaggtttg tactagaata aatatcgttt ctgaagcgtg tttgtaggtc
     2161 agtctagcgg taagtcttaa catttaacgg cgttgtttag attttaagca aaacaaaata
     2221 tataggaaca cgtatcatga tattaggata taatgactaa aataatagca gtaggatggt
     2281 ttttaattgc aaatcatctt actgctgttt ttaattatgc taatttgcga tgcggctatt
     2341 ataaggacag agttgtttat taattatggt gatttagaaa tatgaagttc aatatgcaaa
     2401 gtcatcgttt gttttaatat gcggaacaat cattaaagtt attgcgattt tttgaactta
     2461 atgaaactaa acaataaatt tgagatactt ttttgtcatt tttatgtaac taacacaata
     2521 atctcgtaca ttattaaaat tttctatatg ataggaataa agcaaagcgc gagtgtgctg
     2581 taaaagtttt ccaaggtgat attacataaa gctataaagg gtaaagatta atgagttgtc
     2641 atgtaaatga cgatgatgta taaatcatgg ttaattacgg aagcattaat attaacctga
     2701 gaagctataa agaattattt ttaaaagcga caatattaaa tacgacgcat ttatttagga
     2761 gtggcaaacg tatgaatggg aaaaaggcga atacgataaa cagatacaaa tattttcatc
     2821 atgtcaatca tcaaaaaatt caacaaagtt ctaaaaagac gctgtgggca tcactaatca
     2881 tcacattgtt atttacagtg attgaatttg tcggaggttt agtatctaat tcattggcat
     2941 tactgtcaga ttcatttcat atgcttagtg atgtattagc acttggttta tctatgttgg
     3001 ccatttattt tgcaagtaaa aagccgactg cacgatacac atttggatat ttaagatttg
     3061 agatattagc tgcattttta aatggtttag cattaattgt aatttcaatc tggattttat
     3121 atgaagctat tgtacgtatt atttatccgc aaccaattga aagtggcatt atgtttatga
     3181 ttgctagtat tggtttactc gtcaatatta ttttgactgt tatccttgta aggtctttaa
     3241 aacaagaaga caatatcaat attcaaagtg cattatggca tttcatggga gacttattga
     3301 actctattgg tgtcatcgtt gcagttgtat tgatttactt tacaggatgg cgcatcatcg
     3361 acccaatcat tagtattgta atttcactca tcattttacg tggtggttat aaaattacgc
     3421 gtaatgcgtg gttaatttta atggaaagtg tgcctcaaca tttggatact gatcaaatta
     3481 tggcagatat taaaaacata gatggcatat tagatgtaca tgaatttcat ttgtggagta
     3541 ttacaacaga gcattattca ttaagtgccc atgttgtgtt agataaaaaa tatgagggtg
     3601 atgattatca agcgattgat caagtatcat cattgttgaa agaaaaatat ggcattgcac
     3661 attcaacgtt gcaaattgaa aacttgcaat tgaatccatt agatgagcca tacttcgaca
     3721 aattaacata aataaaacat tgtagcgcct aaaacattaa tctatgtcat aggcgcacgt
     3781 ttcgttttat acttatgttg catcatttaa atgattttcg tcaatttctt tgatgctatc
     3841 tacatctaac acgacatctt taggtttcaa aatatgaata tgtttttcat catttgtatg
     3901 taaaatgcgt tctatgatgt acctttgacc ggccattgtt tctacagcaa tctttttgtt
     3961 tctagctaaa cttgctacga cagattcttt atccataatg atagc
//
LOCUS       DAKTGS010000177         3957 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.182, whole genome shotgun sequence.
ACCESSION   DAKTGS010000177 DAKTGS010000000
VERSION     DAKTGS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.182"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..533)
                     /locus_tag="O0286_002623"
     CDS             complement(<1..533)
                     /locus_tag="O0286_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCY0170957.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKL"
     gene            complement(688..1182)
                     /locus_tag="O0286_002624"
     CDS             complement(688..1182)
                     /locus_tag="O0286_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0170958.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(1581..2003)
                     /locus_tag="O0286_002625"
     CDS             complement(1581..2003)
                     /locus_tag="O0286_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCY0170959.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            2151..2867
                     /locus_tag="O0286_002626"
     CDS             2151..2867
                     /locus_tag="O0286_002626"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCY0170960.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            2950..3489
                     /locus_tag="O0286_002627"
     CDS             2950..3489
                     /locus_tag="O0286_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCY0170961.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(3639..3950)
                     /locus_tag="O0286_002628"
     CDS             complement(3639..3950)
                     /locus_tag="O0286_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0170962.1"
                     /translation="MKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVEQYN
                     DYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLAETF
                     K"
BASE COUNT         1388 a          594 c          642 g         1333 t
ORIGIN      
        1 aataattttc cggttttatc aattgatcca accatttttc ctaaaattgc gagtaaaaag
       61 aaccataacc atgaaacgct aatacaagcg attgtaaacg caattttatt gctgccacta
      121 tataatgcag cactactacc aattactcca attgtatcta aaatagcatg tggattgagt
      181 aatgaaactg ataaagcaaa acttacttgt ttcattggag acataatttg agtttctcca
      241 tctgttgagg gtttatcatg ccaaatggtc caagccatat acatcaagaa aattaaacca
      301 actatataaa taattgcttg aagtacaggt aaagacataa taatgataga tactcctacc
      361 actgcaataa taataagtaa gctgtctgac aacccggctg taattattgc aggcaataca
      421 tatctatatt ttggttgatt agctccttgg ttaaaaataa atacattttg tgcacctaaa
      481 ggtaaaatta gaccaatcgc taagataaat ccatgaataa ttgcggttac cattaagttt
      541 tgacacccct aaaacataga atattctgaa aaaacttatt gttatcatat caaataaaaa
      601 acaactactc aatagtagag tagttgtatg aaaaatagaa cctatatgat ttgaatttat
      661 gagaaatgta gaatatataa aactgtatta cttattaatc aaatcataaa aaagccaatg
      721 ttcatcgaaa taggtatttc cgattttaat tgcatttttc tcaaaaccaa gaatgtcgaa
      781 tcctagtgca ctatagaaaa ctttagcacc tatgttgttt gacacaattg atgtaagtaa
      841 agattcgtaa ttgttctgcc gtgcataatt aataatgaaa ttaatgagct cgctattaat
      901 cgatttatcg ttattagtaa caaagttgta tttaattaat gatttatgtt cttttccaac
      961 gtatcgaatt tgttctaagg cagctgttgc gataagtgtt tcgttttcat aacagccaaa
     1021 tacaatacat ttcggtgaac cttgatttaa aatgtcatgt attaaggtgt cagtcattgt
     1081 ttgactataa tgtgaatctt gagtgaattc ttcattgact gtcgaaacaa gtctcttata
     1141 ctcattaaaa tcattattcg ttaatgtttt aatttcaatc attactgacc tccttaatca
     1201 ttattaagaa agattttgta ctaaaaaaat ttcttaatta catattaaaa tgtttattaa
     1261 aattttgcaa tcactgattt atatgcacat tcttgtaaat ggattatcaa tttaattata
     1321 tttattttta agttcaggaa attatggaat tggttattgg tcgacactgg acttttattt
     1381 gtagacggta tgcgaagaag tcaagccagt attggaaccg gtcacattta tgatgactaa
     1441 ggtacaatca ttttcagtta aatattgcca gaaatttcaa tcacgaatag aaaaaataac
     1501 caaccaaaaa tttttggttg gcaaatttga attttatgtg aattttatta ataacgttga
     1561 ataatctctt taagttaatg ctaatctaca acatttacat ttaaatcgac attaatattt
     1621 ccttgagtcg cttttgaata tggacaaaat tcatgagcca tttgtaaata tttttcagct
     1681 tcttcttgag atataacatt tttaattgtc gcatcaattg aaacacttaa tttaggactt
     1741 tctgagtctg aatcatcttc tagtctcact gttagtgtta cttctggatg agcatcacgc
     1801 actttgtttt gctttaaaat taggtcgaaa gcaccgttga agcaagatgc ataacctgct
     1861 gcaaataatt gttcggggtt agtagcttta ccatctgctt gagcaggcgg aacgatatca
     1921 atatctaatg ctcgatcatc agtataaaca tgtcctttac gtccgccaac attcgtcgct
     1981 ttagtttcat aatgtattgc catacttata acctcctaat ttaaaataca tttatgcttt
     2041 acccattcga aaatacctta atcattttca tttatagcat tgtttgattg aaggataaaa
     2101 agttgttgtt tacaataaaa ataatgagta tctgaaatga gggattcact atgacacatg
     2161 tggaagtagt agcgactatc gcgccacaat tatctatcga agaaacttta attcaaaaaa
     2221 ttaatcatcg tattgatgca atagacgtat tagaattacg aattgatcaa attgaaaatg
     2281 tcacagttga tcaagtggca gaaatgatta caaagctgaa ggttatgcaa gattcattca
     2341 aattattagt tacgtatcgt acaaagttac aaggtggcta tgggcaattt acaaatgact
     2401 cgtatcttaa tttaatatca gacttagcaa atatcaatgg catagatatg attgatatag
     2461 aatggcaagc agatattgac attgaaaaac atcaacgaat cattacacat ttgcaacagt
     2521 ataataaaga ggtggttata tcacatcata atttcgaaag tacgcctcca ttagatgaat
     2581 tgcaatttat attttttaaa atgcaaaaat tcaacccaga atacgttaaa ttagcagtaa
     2641 tgccacataa taaaaatgat gtgttaaatt tattgcaggc aatgtctaca ttttcagata
     2701 ctatggactg caaagttgtt ggtatttcaa tgtctaaact tggactaata agtagaacgg
     2761 ctcaaggcgt ttttggtggt gcgttgactt atggttgtat cggagtacca caagctccag
     2821 gacagattga tgttactgat ttaaaagcac aagtgacttt atactaataa aatgaattag
     2881 gttgcttttg tgtaagtgct gtcttataat tgagaagtat tatcattgtt cgtggaggag
     2941 cgattttaga tggaattaca acaagcaata gctaatagaa gaagtgtgaa aaaatttaaa
     3001 agagatatgc acatagatga cgcattgcta tatcaagcaa ttgagaaagc tgctgatgct
     3061 ccaaatcacg gaatgaggga accatggaga gttgtgcatg ttccgaaaga cagattagga
     3121 gatatgagta aggatatttc taaatttgca tttcctaatg aattagataa gcaacaatgt
     3181 cattatgatg cagttacgaa actaggtggc atgttattgc ttattttaaa aacagatcca
     3241 agacaacgtc aaaatgatga aaactacttt gcatttggtg catatgcaca aaatcttatg
     3301 ttgttacttt atgaagcggg aataggtaca tgttggaaat cgccattata tatctatgat
     3361 cctaaagtaa gaaaaacact tggtataaag aaagatgaag ttcttgctgg attcttatat
     3421 ttaacggatt tagaaggaga tatgcctaaa gcaccacgta aaaatagaaa cttaattaca
     3481 ttatattaat atgtataatt atagaaacat taataaaagc tgagtcatga attgatggac
     3541 atctatcgag ttagagattt aatctaactt actagagtcg gtacaataac agtctcagct
     3601 ttttattgtg cagtatatac acatttttat tttagtattt atttaaaagt ttctgctaaa
     3661 aatgattcaa cttgttcagg tgacttagca tttgctgaat gaaggtgtgc aattttatcg
     3721 ccgtttttaa atactagcaa gctagggata cccataactt cattttcaac aactacatct
     3781 tctaattcat cacgattaac agtataccat tggtaatcat tatattgttc tacgattggg
     3841 tcaatccata aatccatagc acgacagtct gggcaccatc ctgcctcaaa tttaacaatt
     3901 acaggtgtat cgctattaat tacagattta aatgattcat tacttttgat tgattgc
//
LOCUS       DAKTGS010000178         3936 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.183, whole genome shotgun sequence.
ACCESSION   DAKTGS010000178 DAKTGS010000000
VERSION     DAKTGS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.183"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            234..1754
                     /locus_tag="O0286_002629"
     CDS             234..1754
                     /locus_tag="O0286_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0170963.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(2123..2818)
                     /locus_tag="O0286_002630"
     CDS             complement(2123..2818)
                     /locus_tag="O0286_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0170964.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(2818..>3936)
                     /locus_tag="O0286_002631"
     CDS             complement(2818..>3936)
                     /locus_tag="O0286_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCY0170965.1"
                     /translation="TYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHID
                     NTTQQHEQALSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINP
                     YDASILESMLNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIM
                     GNLDILVSHSKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYL
                     VSELVEEIDMILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDT
                     KINLSIRYASYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLY
                     LVQTILHKHQSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
BASE COUNT         1316 a          579 c          702 g         1339 t
ORIGIN      
        1 ataaaaaatt ttttataatg attattcgaa tgatgcgtaa cgcttacatc ttatctaatg
       61 ctagcttttt gacaaaaata tgacaatcaa ttaatgtgat tctaataaat attcgcaaat
      121 tgctttattg cgattaaatt tttttggtgg tactatatag aagttgatga aatattaatg
      181 aacttatatg caaaagtata ttgagaaata aacaggtaaa aaggagaatt attttgcaaa
      241 attttaaaga actagggatt tcggataata cggttcagtc acttgaatca atgggattta
      301 aagagccgac acctatccaa aaagacagta tcccttatgc gttacaagga attgatatcc
      361 ttgggcaagc tcaaaccggt acaggtaaaa caggagcatt cggtattcct ttaattgaga
      421 aagtagtagg gaaacaaggg gttcaatcgt tgattttagc acctacaaga gaattggcaa
      481 tgcaggtagc tgaacaatta agagaattta gccgtggaca aggtgtccaa gttgttactg
      541 tattcggtgg tatgcctatc gaacgccaaa ttaaagcctt gaaaaaaggc ccacaaatcg
      601 tagtcggaac acctgggcgt gttatcgacc atttaaatcg tcgcacatta aaaacggacg
      661 gaattcatac tttgatttta gatgaagctg atgaaatgat gaatatggga ttcatcgatg
      721 atatgagatt tattatggat aaaattccag cagtacaacg tcaaacaatg ttgttctcag
      781 ctacaatgcc taaagcaatc caagctttag tacaacaatt tatgaaatca ccaaaaatca
      841 ttaagacaat gaataatgaa atgtctgatc cacaaatcga agaattctat acaattgtta
      901 aagaattaga gaaatttgat acatttacaa atttcctaga tgttcatcaa cctgaattag
      961 caatcgtatt cggacgtaca aaacgtcgtg ttgatgaatt aacaagtgct ttgatttcta
     1021 aaggatataa agctgaaggt ttacatggtg atattacaca agcgaaacgt ttagaagtat
     1081 taaagaaatt taaaaatgac caaattaata ttttagtcgc tactgatgta gcagcaagag
     1141 gactagatat ttctggtgtg agtcatgttt ataactttga tatacctcaa gatactgaaa
     1201 gctatacaca ccgtattggt cgtacgggtc gtgctggtaa agaaggtatc gctgtaacgt
     1261 ttgttaatcc aatcgaaatg gattatatca gacaaattga agatgcaaac ggtagaaaaa
     1321 tgagtgcact tcgtccacca catcgtaaag aagtacttca agcacgtgaa gatgacatca
     1381 aagaaaaagt tgaaaactgg atgtctaaag agtcagaatc acgcttgaaa cgcatttcta
     1441 cagagttgtt aaatgaatat aacgatgttg atttagttgc tgcactttta caagagttag
     1501 tagaagcaaa cgatgaagtt gaagttcaat taacttttga aaaaccatta tctcgcaaag
     1561 gccgtaacgg taaaccaagt ggttctcgta acagaaatag taagcgtggt aatcctaaat
     1621 ttgacagtaa gagtaaacgt tcaaaaggat actcaagtaa gaagaaaagt acaaaaaaat
     1681 tcgaccgtaa agagaagagc agcggtggaa gcagacctat gaaaggtcgc acatttgctg
     1741 accatcaaaa ataatttata gattaagagc ttaaagatgt aatgtcttga gctctttttt
     1801 gttttcaata attgattctc tgtagatatc aaagtactaa cgttttaaag gttaaatatt
     1861 taattggatt gagatctgta tgcggttata tcattctgtg taaatatggt tctccaccaa
     1921 atgtggtgag tatataattt aaagaactat ttttaaatta agaataatcg aacataaata
     1981 aactttatga aatttcagta tcatgttctt ataaaaaaca atagggcttt ttgctgacgc
     2041 tagtgcgcga taaataataa gttgaatata aaaaagatca ctgccaatca ttcgtttaat
     2101 ggcagcgatc ttttttattt aattatttct ctttccactg caacatttga taaccaatgc
     2161 gtggatgtgt tttaataata tcttttgcgt cctcatgaca ttgtgaaagt ttttgtctta
     2221 atgatgtcat atgcactctt aaactaggca tttcagtctt attaacatag ccatatattc
     2281 gttttaatat catctcataa gtgagtactt ttcctttatg atttgacaat agttctaaca
     2341 agctaaattc atttggcgtc aaatgtacct cctgattatt aataacaaca gatttggagc
     2401 caaagtcgat gcttagcaaa ccgttagtaa atacaatgtt agtttcttga tgtgacttag
     2461 cgattctctc gatgactcgt attcgtgccc gaagctcatc aacattaaaa ggtttagtca
     2521 tatagtcatt cgcaccgtta tctaaagctt gaataattgt ttgttcttct tgtcttgcac
     2581 ttattacaat gataggaatg tcagtatgtt gcctgatttc tgaaatcaaa cataatccat
     2641 ctttatctgg taaacctaaa tctaataaaa tgacatctgg tttatcaatt tgaattttaa
     2701 agtgtgcttg tgtggcattg tcggctgtag ttacattgta ataatctaaa gttaatgcaa
     2761 catcaagtaa atgtgtgatt gcgtgatcat cttcaattat caatatttta gattgcatta
     2821 tacgtctcct tcgttaaagt ctgtatatat attgaaatag aatatactgc cgtgtggttg
     2881 gttcggttta tattgtaagt ttgattgatg tttgtgtagg atagtctgta ctaaatataa
     2941 gcctagtccc atgctttctt tttggttatc tttaaaatat ttatttgatc ctgtgtaaaa
     3001 aggctcgaat atcttttgtt gttcttctaa actaattcca ggtccttcgt ctataacggc
     3061 aaattcgatt tgttcatagc tagcataacg aatagataaa ttgattttgg tgtcagtaga
     3121 agtgtgttta actgcatttt caatcaaatt gaataaagct tgtaaaatca acttactgtc
     3181 aatgtgtata aactgtaaat ttactgagga tgatacagtt atacgctttt ttaaatggcg
     3241 acgttctaaa atcatatcga tttcttctac taattcacta acgagataag gttgcaattt
     3301 tatctgaaca tttgatgact gtaattttgt taatgataaa atatttgtca ctaatagata
     3361 taaatactga ctttcttgaa aactatgtac aagtaattgt tccttttcta tgatagacat
     3421 atctttacta tgtgatacta aaatatctaa atttcccata attgttgtta acggtgtacg
     3481 tatgtcatgc gaaattgatc ttaaaaaatt tgaatgtgtc agttgacgtt cagcctgtaa
     3541 catggattct ctcgtttgtt taagtaacgt cacattttca acggcgagag aaagttcatt
     3601 taacattgat tctaatattg atgcatcata tggattaatc acttgagaac tttggtaatc
     3661 aatggctaga atgcctttaa tcggagatgt gccaattggt atcaaccatt tattaatgcc
     3721 tggaaatgta tctgttgttg caccagcttg tctttcattt ttaattaccc agcttaatgc
     3781 ttgttcatgc tgttgagtcg tattatcgat atggttttgc aatggtattg ttttaattac
     3841 tttcgattga ttgataacgt atatagtaat tgattgttgc aataattgat taatttggta
     3901 tccagcattt attagtaagt tttcaactgt ataagt
//
LOCUS       DAKTGS010000179         3903 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.184, whole genome shotgun sequence.
ACCESSION   DAKTGS010000179 DAKTGS010000000
VERSION     DAKTGS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.184"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..1218)
                     /locus_tag="O0286_002632"
     CDS             complement(<1..1218)
                     /locus_tag="O0286_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCY0170966.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGI"
     gene            complement(1436..1963)
                     /gene="pyrR"
                     /locus_tag="O0286_002633"
     CDS             complement(1436..1963)
                     /gene="pyrR"
                     /locus_tag="O0286_002633"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCY0170967.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(2362..3279)
                     /locus_tag="O0286_002634"
     CDS             complement(2362..3279)
                     /locus_tag="O0286_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0170968.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(3279..3770)
                     /gene="lspA"
                     /locus_tag="O0286_002635"
     CDS             complement(3279..3770)
                     /gene="lspA"
                     /locus_tag="O0286_002635"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCY0170969.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
BASE COUNT         1309 a          732 c          539 g         1323 t
ORIGIN      
        1 gataccaatt tcttttaagt taaatactaa attaccgata cctacaacta aaattacaga
       61 agctataact aaatttcgat tgttcgcaaa atctactttg ctttcaacta acattcttaa
      121 gccacttgct gcaataatac cgaaaagtaa tatagatact cctcccataa ctggtgtagg
      181 tatagaagaa attaatgcag tgaacttacc aatgaatgct aaaacaattg ctataactgc
      241 tgcaccacca atgacgtaaa tactgtatat tctggtaatc gctaatacac cgatattttc
      301 accgtatgtt gtacttggtg gtccaccaat aatactggca aacattgtag aaacaccatc
      361 accaatgatt gatttatcaa gtcctggctt ttcaaagaag tttctaccta cgattttatt
      421 caataccatt tggtgcccaa tatgttcact tactgttacg aacacaatcg gaatcattac
      481 aagtacaagt cctaagtgaa atgaaggtac ataatcttta aatggcagat aaatatgagg
      541 gaaatctatc cattttgctg acataattgc atcaaatttc acaatcccca taaaaatcgc
      601 taccacataa cctacgataa tacctacaag aactggaatt aaagatagga atcctttaaa
      661 aaatccttga acgactattg ttacaagcaa ggttatcatt gcaacaatta agaaactgat
      721 attgtaacct ttcatatctc caggattttc atacattgcc atattgactg cagtaggcgc
      781 taagcttaaa ccaattacca tgatgactgg tccaacaaca actggtggta ataatttcat
      841 taaccatgct gtcccactta atttgattag aatcccgatg atgacgtaca taacaccact
      901 catgaataat gctacaagca tgtctcctaa gctatgcgta cttaatcccg tgataattgg
      961 cgtgataaat gcaaagctag atcccaagta tgctggtatt tgcgccttcg ttattaagat
     1021 ataaagtaat gtaccgattc ccgaagctag taacgctgct gatattggta gtcctgttaa
     1081 gaatggtact agtactgttg cgccaaacat cgcaaataaa tgttgtaagc ttaaaaatgc
     1141 ccattgcgct ggttgtggtt tttcatttac atctagtacg ggttttactg ttcgttcaaa
     1201 catttcatca ttttgcataa tattcatttc ctccgataat aaaaaaatct ctttacatca
     1261 gtatatgtaa agagacaaaa agtgtgacaa gttgctacaa gtcattttcg tccatagaaa
     1321 ttgacttata gttgtcgaac atgagggtat tattagataa acaagcatat gaaaacttat
     1381 ttatcattca actcccccac ctttttcagt ctctcgtact gaattaaaag gggtattatt
     1441 taattataac tgcatttctt tgatccattt cttctaaata gacacttacc gtttcctctt
     1501 tagaagtagg tatattttta ccaacaaaat ctgctcgaat tggtaactca cgatgtcctc
     1561 gatcaaccaa agcagctaaa ccaattttaa taggtctagc atttagcaaa atagcatcaa
     1621 gtgaagcacg aaccgttcga ccagtataca gcacatcgtc aataatgatg actactttat
     1681 ctgtaatatc tgtgtcgatg tctattgcgt cttttgtcgt aagtgatgac atgtgctcta
     1741 tatcatctct aaagtatgta atatcaattg ttccagtagg tatacgttgt tgctcaattt
     1801 gatgaatttt atcttgtata cgattcgcta aatattcacc tcttgttttg atacctaaaa
     1861 gaattaaatt atcagtacct ttgttatact ccaaaatttc gtgagcgatt ctcgtcactg
     1921 tacgttgtat tgcggcatca tccattatga tacgttcaga catattctca ccctttcaaa
     1981 atttagaaac aaaaaacctc atgtctttaa tatcgacatg aggtttagta tatacaatca
     2041 gcctatttac atagaagaat cgtagtatta ctacttattc ttgcatatgt gaaagtgtta
     2101 tgatcatttt agtatatatc tatattttca tgtctttgac gtctctctgg acgctcaatt
     2161 aaagactctt tatgttgtac ttaaactgtt ataaggataa tactaatcag gcgctttgtc
     2221 aatctatatt gaaaaattaa ccatactcag taaaaatgtt aaccttaatc tattttataa
     2281 tgtgttttca tttaaaaaaa cgaaaacgat aaacacttat agtttacata ataatgttat
     2341 cgtaattata gcacgacaca attatgcatc tctttttcga attgtatcta ataaatcttc
     2401 aaagtcttgt ggtaattcag catgtctttc aatatattca cttgttactg gatgttcgaa
     2461 tccaataagt ccagcatgta gagcttgacc accaatatcc aatgtctttt tcggtccata
     2521 ctttggatca ccaactaatg ggaagccaat atatttcatg tgaacacgga tttgatgcgt
     2581 acgtcctgtt tcaagttgac attcaacaag cgtataatct ttaaaatgtt ctagtacgtt
     2641 aaaatgtgtc actgcttcct taccatcatc aacaacagcc atagattgac gatcattttt
     2701 gtttctacca attggcgcat cgattgtacc gtaatcatga ggaatattcc cgtgaactaa
     2761 agcgatatat tttcttttaa cagatttatc cattaattgt tctacaagcc cacgatgagc
     2821 aatatcattt ttagcaacca ttaataaacc agaagtatcc atatctatac ggtgaacaat
     2881 acctggacga atttctccat taatacctga caaattttta atttgataca ttaaaccatt
     2941 aactaatgta ttggtataat gccctggtga tggatgaact accatgcctt tcggtttata
     3001 tacaactgca acatcgtcat cttcataata aatatctaaa tttaaatttt caggtagaat
     3061 atcagcttca accacttctt tttcagtgac aactatatga tcattaagtt tcactttata
     3121 attagattta acaactttat cgtttgcaac gactaaacct gctttaatcc aatcttgtat
     3181 ctggttacga gaccaatcat tatttaattc aggcagcaac ttatctacac gcatacctgt
     3241 ttgttcttta tctgtaatgt taaattcata agtctccatt acttaacctc cttctccttt
     3301 ttattggaag tatcctttaa taaggcaata ataattaata ttacaccaat tgttaaactt
     3361 gaatctgcga tattaaatat tggaaaatca taaccaaaaa tatttgtatc aataaagtca
     3421 acaacttctc ctgttaaaat tctatcaata aagtttccaa gtgcacctgc aaaaagtaaa
     3481 ctaatagcaa cttgcataaa caaattatat tgagcatctt taataaagaa atatactaag
     3541 gctattaata taataatggt aataataaag aaaaatgtca tttttccact caatattccc
     3601 catgcagcac cattatttcg atgtgatgtt atgtttaaaa agtgcggtat cacttcaaat
     3661 gaatctccaa ttttcattgt agtagctata atatatttag taacttggtc aaatataacg
     3721 acaaatactg ctattaaaat ggaagtgcca ataaaatatt ttttgtgcat tttcgttcct
     3781 ccaatcaatc gtccatgaga caactcttta tattatagct tacacctgct aataaaaaaa
     3841 gtaagcatat tacattaaat ctaatgttac taactcaata cttgataaac tactatgttt
     3901 tga
//
LOCUS       DAKTGS010000180         3810 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.185, whole genome shotgun sequence.
ACCESSION   DAKTGS010000180 DAKTGS010000000
VERSION     DAKTGS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.185"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(2..1015)
                     /locus_tag="O0286_002636"
     CDS             complement(2..1015)
                     /locus_tag="O0286_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0170970.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            1327..2469
                     /locus_tag="O0286_002637"
     CDS             1327..2469
                     /locus_tag="O0286_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0170971.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            2470..3588
                     /gene="mnmA"
                     /locus_tag="O0286_002638"
     CDS             2470..3588
                     /gene="mnmA"
                     /locus_tag="O0286_002638"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCY0170972.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
BASE COUNT         1314 a          571 c          732 g         1193 t
ORIGIN      
        1 attataaagt gtttttttca tttatctgat ttataacatt cttgaaccga cgataagagt
       61 ctaattgcat atctttatca taaatataac ttattgccat aatttcgttg acttcaccat
      121 aagtttccat aaaatcttga attttttgtt gaacagtatt ttctgaacct attaatgata
      181 ttttctgtct ttccatagcc atcgcgtatt ctctcggcgt taatatattt tgtagttcat
      241 gtgttggcgg ttgaacgggc tgcattctgc cacgagtgat actaaccatt acttgtgcca
      301 tagatgaagc taaatattgt gcttcgtcat cattttcagc aacgattgta tttaaacata
      361 caataacata aggttcgtct aatacatcag aaggttcaaa taacgttttg taaatttcga
      421 tagcttcttt catttgttga ggtgcaaaat gtccagcgaa cacatatgga agccctttgc
      481 gagcagctaa atgtgcagaa tctgttgaag aaccaagaat gtataaaggc acatttttac
      541 ctactgctgg ataagcacga acatatgctt gttggtgagc agggccgaaa tattgttgta
      601 ataatgaaac ctcttctgga aatttataaa caccatcatg ttgatctcgt cttaatgcac
      661 ttgcggtcat catatctgtt ccaggtgcac gtcctaatcc taaatcgaca cgatttggaa
      721 ataatgttgc catcgtgcca aattgttccg caacgattaa tggagcatga ttaggtaaca
      781 tgatgcctcc agaacctaca cgtatgtgtt tcgtatgttc taacgtatgt tgaattaata
      841 aagcagttgc tgaacttact aggttgggag cgttatggtg ttcagcaatc caatatcttt
      901 catatgataa ttcgtctaaa tgttgtgcga gattaaccat atcattaatt gctgtttgtt
      961 catcttcacc ttctcgaata gggacaaggt ttaatgctga atatttaatc gccattttta
     1021 tatcgtcctt ccttttttat gatataagaa gtataccaat cgaataacca gataaatatt
     1081 aaattgctca taaccaaaat aatagtacgt tacattttac aaataaaaaa atatctgcaa
     1141 atactaaaat ttataaaaaa gacaagaata aagcgataaa cttaaaaaag cctatcgaaa
     1201 agcatgtgta gaaaatggta aaatataatt aaaaacatta taaataaagt taaacagaaa
     1261 agtagaaaaa tagtatgtaa ggtgctatta ttggaacatt gataaaattg tgaggagtgt
     1321 ccaaacatgg aaatttatgc agattatgct gcgaccacac cagtaaaacc tgaagtagta
     1381 gatgcgatga tgacgattta caattcgcat tatggtaatc catcatcaat acatgctaag
     1441 ggaagagatg cgcgtaaata tttagatgag tcaagacgtc aaattgcaca attacttggt
     1501 gcagatacac atgaaattat atttacgagt ggcgcaacag aatcgaataa tacggcaatt
     1561 aaaggcatag taaaagcaaa tgaacaattg ggtaatcaca ttatcacatc gaagattgaa
     1621 catcattctg tgttacatgt ttttgaacaa ttagagcgag aaggttttga tgtaacatat
     1681 ctagatgttg atgatacagg tgctattgat ttagatcaac ttgaagaaac aattacagat
     1741 aagacgattt tagtatctat tatgtttgta aataatgaag ttggcacagt gcaacaaatt
     1801 tatgatattc aagatattat tgctgagaca aatgcatatt tccatgttga tgcggttcaa
     1861 gcaattggac atcttgatgt taagtttgac gaatttgaga tcgacgcaat gagtattact
     1921 gctcataagt ttggcggacc aaaaggtgtc ggtgcattac tagttaaaga ccatgttaca
     1981 ctagattatc ctcaacttgg cggtgaacaa gaattgaaac gccgtgcagg cactgaaaat
     2041 cttgcgcaaa ttgttgggat ggctaaagca ttacaattag cagaaaagaa tcgagacgat
     2101 aacaatattc atttaatgaa tttaaaagaa caatttttag ttaaattaca agaaagagcg
     2161 attccatttg aattaaatgg ttcaatgacg gatgctacag gccatattgt aaatctatat
     2221 tttccgtttg ttgaagttga aacgatgtta acattgttgg atatggcaca aatctatgtg
     2281 tcgtctggat ctgcatgtac agcaggctct acacaaccgt cgcatgtttt agatgcaatg
     2341 ttcgaagatg aggagcgatc aaatcattcg attcgattta gttttaacga attgactact
     2401 gaaaatgaaa ttaatgcaat tgtagctgaa attcataaaa tatattttaa atttaaggag
     2461 gagtcataat tgtcaaataa agatatacgt gttgtcgttg gtatgtcagg cggtgtagat
     2521 agttctgtaa cagcccacgt cttaaaagaa caaggttatg atgtcattgg catatttatg
     2581 aaaaactggg atgacactga cgaaaatggc gtatgtactg caactgaaga ttacaacgat
     2641 gttattgaag tgtgtaatca aattggcatt ccgtattacg ctgttaattt tgaaaaagaa
     2701 tattgggata aagtctttac gtatttctta gatgaataca aaaaaggtcg tactccaaat
     2761 ccagacgtta tgtgtaataa agaaattaag tttaaagcct ttttagatca tgcgatgaat
     2821 ttaggtgcag attatgtagc aacaggacat tacgcacgca tacatcgtca tgaagatggt
     2881 catgttgaaa tgttacgtgg tgtagataat aataaagatc aaacatactt cttgaatcaa
     2941 ttatctcaac aacaactttc aaaagtgatg ttcccaattg gcgacatcga aaagagtgaa
     3001 gtgcgtcgaa ttgctgaaga acaaggactt gttactgcta agaaaaaaga ttctacaggc
     3061 atttgtttta tcggcgaaaa aaactttaaa acatttttat cacaatattt acctgcacaa
     3121 ccgggtgata tgataacact tgatggtaag aaaatgggta aacatagtgg tttgatgtat
     3181 tacacaatag gacaaagaca tggattaggt ataggtggag atggcgatcc ttggtttgtt
     3241 gtcggtaaaa acctaaaaga taatgtttta tatgtagaac aaggattcca tcacgatgca
     3301 ttatacagtg attacttaat tgcttcagac tattcatttg taaatccaga agataatgac
     3361 ttagatcaag gttttgaatg tacagctaaa tttagatatc gccaaaaaga tacgaaagtt
     3421 tttgtgaaac gtgaaaacga ccatgcacta cgtgttactt ttgctgagcc agtaagagca
     3481 atcacacctg gacaagcagt tgttttttat caaggtgatg tgtgtcttgg tggtgcaaca
     3541 attgacgatg tgttcaaaaa tgaaggtcaa ttaaattatg ttgtataaac aatggcaaca
     3601 ataaattact tatttgaagt ttcaacgttg aaaatgacga aagacagttt ttgatgagaa
     3661 taattcatga ggatagagtc tgggacatca caatgtccta ggctctacaa tgttatattg
     3721 gcgggaccac aacatagaga atttcgtaaa gaaattctac aggcaatgcc agttggggat
     3781 aacgaattta attttgttaa aatatcattt
//
LOCUS       DAKTGS010000181         3761 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.186, whole genome shotgun sequence.
ACCESSION   DAKTGS010000181 DAKTGS010000000
VERSION     DAKTGS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.186"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..310)
                     /locus_tag="O0286_002639"
     CDS             complement(<1..310)
                     /locus_tag="O0286_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0170973.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     L"
     gene            complement(321..1244)
                     /locus_tag="O0286_002640"
     CDS             complement(321..1244)
                     /locus_tag="O0286_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCY0170974.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            complement(1219..2337)
                     /locus_tag="O0286_002641"
     CDS             complement(1219..2337)
                     /locus_tag="O0286_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0170975.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            complement(2680..3711)
                     /locus_tag="O0286_002642"
     CDS             complement(2680..3711)
                     /locus_tag="O0286_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCY0170976.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
BASE COUNT         1193 a          788 c          534 g         1246 t
ORIGIN      
        1 ttaatacaga aataaaaact aatggcagta atacgtttaa aaagaacgta aagccatttt
       61 tattttgtat atctccaaaa acaaaattta tgcctgcttt actaatattt attagccctt
      121 caaaaaatga acctagtgca gttaaaattg tcaaaccaat cgttgtgttc atcataaata
      181 acacgatcaa cacttgaata aatatcatta ttaacgtctt tttgaagtct atttttttac
      241 gatcaaaact gaataaaaac gcaacaaata gtgcaaatgc tatccctgtg atagcgaata
      301 aaatattcat gtatcatcac ctacaataat ttattaacag cgacagcaat tttagcaccc
      361 aacgccgcat tgttttcaac aagttttata tttgctgcta aacttttacc attcgttttt
      421 tctacaattt tccctaacaa gaacggtgtg gcgtccttac ctttaatacc ttgattttcc
      481 gcttcaacaa cagcttcatt tatgattgcc tcaatatatg cttttgataa ggcatgctca
      541 tatggaattg gattagcaac aacaatgcca ccttcaagat ttaactgctg ttttgttaaa
      601 tgaatgtcag caagtcgttc tggcgtttca accgaacttg ttaacttaac accgctttcg
      661 cgagtgaaga atgctggcaa ttcattcgtt tgatatccaa taactggaac gccttttgtt
      721 tctaaatact ccatcgtctt aggtaagtct aaaattgatt tggcacctgc acagataaca
      781 gtgacatttg ttttagacag ttcttctaag tctgctgaaa tgtccatcgt atgttctgca
      841 cctttatgga cgcccccaat acctcctgta acaaaaaatt gaataccagc cattgcagca
      901 catatcatcg tcgtcgctac agtagtagca ccaacacact tcatcgcaat aacttctgct
      961 aaatcccttc tagatacttt agcaacgtct ttactagttg ccagtatttc taaatcttcg
     1021 ctttctaaac caattttaat tttgccatct ataatggcta tggttgctgg aatggcacca
     1081 ttattcctga taatttgctc tactgttgtt gccatttcaa cattttgtgg gtacggcata
     1141 ccatgcgaaa taattgttga ttctaatgct acaatcggtt gattgttctc ccgtgcttgc
     1201 tgaacttctc gagaatactc aatatacttt tgtaaatttg ccatttttat aatcctccat
     1261 atcgtgataa agttgctgtt gatctaggtt ttgcctaact gtatatttcg tttctatcgt
     1321 tttctttgcg ttaaccatac cagcaattaa tatatcttca gtagacatcc catttaacca
     1381 gctatacact actgcagcac agaatgaatc gcctgcacct gtaacatctt tcacactatt
     1441 tgatggcata actgacttaa tgatttcttc ctcaccactt cgataaatga gttctttcac
     1501 gccatttgtc acaataacat ttttaacacc taaatcattc cagcgtttag cagctatttt
     1561 taaatcatca gtagattcta tttttaaatt taagtatgtt tctgtttcat ctttattcgt
     1621 gataatccaa tcaatagcat gtaatgaatc aggcatattt ttcatttttg gggaagaaac
     1681 cgtggtgata actaatttga tttgatgttt cgtggtatag gcacataaga agtttaatgc
     1741 ctctttgcct aaattcaaat ctacaataat gcacttagcc tttttcaata agtgtgaacg
     1801 cttaattaaa aattcaggcg taatgtagtc aaacacttcc atatctgcta agccatatgt
     1861 catgtcgcct tctttactaa ttaaagctgt atatgaacct gtactcgcat tttcaaattg
     1921 ttgaacatga tccaaattca taaatggtgt ggacaatcgt ttaatcattt cccattcact
     1981 atcttgtcca ctagcagata aaaaagcgac cgtttcgcca agcctaccta agttctcagc
     2041 aatatttctt gctacgccac caatagagcg tgttgacgtt acaggatttg atgtttctgc
     2101 aactaaattt ttatgcacat aaaacttacg atctacattc gctgcgccaa tacaaacaat
     2161 aggataatct tcatttaaaa catatgcctt tcccataaca tattcctttt gtattaatcc
     2221 tgaaataatg tttgctacgc tgggtctaga taatccaatt gcctcagcaa gttcacgttg
     2281 tgaaataaac ggattatctt taattctttt taaaatttct ttctcagaat cgctcataac
     2341 atccctcctc atattgactt aaaatacttg aatcagtgaa tttacaaaaa tatcgtagca
     2401 tgctgttatt tctgttgtaa acgcttacat ttaccgttat atcaatgata tttgtttata
     2461 aaatacgcat aatttcgtaa aaagtaagtt cattccatcg tgtaaaaggt acttgagaaa
     2521 atgataaatg tttatacagt atgatatttg tcgacaataa attatgacat ttttatgtct
     2581 tgtatttttc tataaaaaaa cgtgactcat tatcgactgt tacatcgact gaatcacgtt
     2641 actatgtttt atttgttttt actattcaat atgatgatgc tatgcgtcta ctgcacctcg
     2701 attaatcatg tcttgcttag taacaattgg atatttttta aatacaattg aaccaagata
     2761 acctgcaagc gcacctacaa tcgccattac tacaccataa atcacaattg tcgtcggatg
     2821 attaaatcca aacataacta aaaatcctgc aatcggtgta gctgtacctg tcgcatcgtt
     2881 aattaaacct gacatagcaa taattaaacc agcaatcgca ccaccaaaga aatttgtaat
     2941 atagattgga attggattgg ctgatacaat atctgcttgt gataaaggtt caatacttac
     3001 tgcaatcgta gacttacgat cacctaattt taagcgatgg aatagcgtcc cattcataaa
     3061 tgccgaacta aatgctgcca tggcaccaat agccataggt acacccgtta aacctaataa
     3121 tgctgtcaat gccattgaac tcaatggcgc tgtgccgaca accgtaataa taccacctaa
     3181 aatgataccc atgataattg gattcgtatt cgtactactt tggataatat caccaattcg
     3241 aatcaatgta ctattcacta ctggcgttaa taatactgca ataagacgag ctaatggcgc
     3301 taagatgaca atcgatccaa ttaagtcgac accatccggc acatatttct ctgtatattt
     3361 catcacatat ccaatcatat aacccgcaaa gaaacctggt aataaatcca taccaccgca
     3421 cgcggctgct ataactaatg catatactgg tgacacaccg atagctaatg ctgttaatcc
     3481 agcggcagcg acaccaccta gacctcccgc agcgtctcct agctcttcta aaaatttaat
     3541 accgaatact tcgccaccaa catatttatt aaatgcttcc actaaaaatg ttgcgattgc
     3601 tgcattagct aatgctccca tggcacgcat accattaggc gctttatatg taaataatgt
     3661 aaaaatcact aagaccaaaa ataaaaataa agtacctatc aataaatcca taaccattga
     3721 cccccattac gataatttta tattcaatcc ccatatttaa a
//
LOCUS       DAKTGS010000182         3735 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.187, whole genome shotgun sequence.
ACCESSION   DAKTGS010000182 DAKTGS010000000
VERSION     DAKTGS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.187"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..351
                     /locus_tag="O0286_002643"
     CDS             <1..351
                     /locus_tag="O0286_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0170977.1"
                     /translation="SGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCK
                     VHLHTGRTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYI
                     TIDYKQLLKLFNQL"
     gene            372..1757
                     /gene="mgtE"
                     /locus_tag="O0286_002644"
     CDS             372..1757
                     /gene="mgtE"
                     /locus_tag="O0286_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCY0170978.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            1767..3611
                     /locus_tag="O0286_002645"
     CDS             1767..3611
                     /locus_tag="O0286_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCY0170979.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
BASE COUNT         1258 a          493 c          703 g         1281 t
ORIGIN      
        1 tctggtatta ttgaagctaa tattagacgg tcaaaggata ggattataac tagagaagtt
       61 gcctcggatg gtaaatacgc taaaacatct tatgaagtaa taaatcagaa tgataaatac
      121 agtttatgca aagttcattt gcatacggga cgtacacatc aaattcgtgt acattttcaa
      181 catattgggc atccaattgt gggagattct ttgtatgatg gttttcatga caaaattcat
      241 ggtcaagtac tgcaatgtac gcaaatatat tttgttcatc caatcaataa gaacaatatt
      301 tatattacaa ttgattataa gcaattactt aaattattca atcaactcta attcacacag
      361 ggggtgtaag tatgtcaatg aacacagatg aaaaagagcg tgttcaagag gaattatacg
      421 accaaacttt attagatcaa tatttagaaa atgatgatat tgatcaattt agagatgaat
      481 ttctagcatt acacacatat gaacaaagtg agtattttga agatactacc gatgaaaata
      541 gacaaaagat ttttcaatat ttatcacctg aagaagttgc aaatttcttt gatcaattag
      601 atattgatga cgatgaatat gagttgctat ttgataagat gaatgcgaca tacgcaagtc
      661 acatattaga agaaatgtca tacgacaatg cagtagatat tttaaatgag ttgactaaac
      721 caaaagttgc tagtctttta acattgatga ataaagatga cgcgaatgaa atcaaagcat
      781 tacttcacta tgatgaggat acggccggcg gtattatgac gacggagtat ttatcactta
      841 aagcgcatac gcctgttaaa gaagcattat tattggtcaa agcgcaagca ccagacgcag
      901 aaacaatata tgttatattt gtcgttgatg atgatggtaa attagtaggt gttttatcgc
      961 taagagattt aattgtagct gaaaatgatg cttatattga agatattatg aatgaacgtg
     1021 tcattagtgt gaatgtagca gacgaccaag aagatgttgc tcaagttatg agagactatg
     1081 atttcatggc tgtacctgtt atagattacc aagaacattt gcttggtatc atcacgattg
     1141 atgatatttt agacgttatg gatgaagagg ctagtgaaga ctactctcgt ttagccgggg
     1201 tatcagatat cgattcgact aatgattcaa tcattaaaac agcattaaaa cgtttaccat
     1261 ggttgattat tttaacattt ttaggaatga ttactgcgac aattttaggg agattcgaaa
     1321 aaacattaga aaatgtagcg ctactcgcag cgtttattcc tattattagt ggtatgtcag
     1381 gaaattcagg tacacaatct ttagccgttt cagttcgtaa cattacgaca ggggaaatta
     1441 atgagcaaag taaatttaga attgcattaa gagaagcagg aagtggtgta ttatcgggtg
     1501 ttgtatgttc aacaatatta tttacaatta ttgttgcaat atatcatcag ccacttttag
     1561 cattaatcgt tgcaggaagt ttaacttgtg cgatgacggt ggggacgttt gtaggttcga
     1621 tgattccatt attgatgaat aaattaaata tcgatccagc agtggctagt ggaccattta
     1681 ttacaacaat taatgatatt attagtatgt tgatttattt tggtttagct acatcattta
     1741 tggcttactt aatttaagga ggagttatgg agtttttatc tttagttatt gttgttttag
     1801 cagcgttttt aactccaata attgtcaatc gattaaatat taatttcttg ccagttgttg
     1861 ttgcagaaat tttgatgggg attgtgattg gaaattcatt tctaaatata gtagaaaggg
     1921 attcaattct aaatatttta tcaacgttag gctttatctt tttaatgttt ttaagtggtt
     1981 tagaaattga ttttaaagct tttaaaaaag ataaacgcgc acgtcaagga caaaatgatg
     2041 atgaatcctc aattccaggg catcttaatc tagcgttaac tgtatttgca tttattatga
     2101 ttatttcgat tcttttagcg tatgtattta aatggcttgg attagtggat gatgtgttat
     2161 taatggtcat tatcatttca actatttcct taggcgtagt tgttccaact ttaaaagaaa
     2221 tgaatattat gagaacaact atagggcaat ttatcctatt agtagcagta cttgcggact
     2281 tagtaactat gattttatta acggtctatg gcgcaatcaa tggtcaaggc ggcagtacaa
     2341 tatggttaat aggtatatta gttgttttca cagcaatttc atatatttta ggtgttcaat
     2401 ttaaaagaat gtcattttta caaaaattga tggatggtac gacgcaaatc ggtattcgtg
     2461 cggtatttgc attaataata ttattagtag ccctagcaga gggagttggc gcagaaaata
     2521 tattaggtgc attcttagca ggtgtcgttg tttcattatt aaatccagat gaagaaatgg
     2581 ttgaaaagtt agactcattt ggttatgggt tctttattcc tattttcttt ataatggttg
     2641 gtgtagattt aaacatacct tcattaatta aagaaccgaa attactaatt atcataccga
     2701 ttttaatcgt tgcatttatc atttcaaaat taattccagt catgtttatt cgacgttggt
     2761 ttgatatgaa aacaacgatt gcatcagcat ttttattaac atcaacatta tcgctcgtga
     2821 tagctgcagc caaaatttca gaaagattaa atgctatttc agctgaaacg tcaggtatat
     2881 taattttaag cgcagtcatt acatgtgtat tcgttccgat tattttcaaa aaactgtttc
     2941 cagttccaga tgagtttaac cgtaaaattg aagttagttt aattggtaaa aatcaattaa
     3001 cgattcctat agcgcaaaat ttaacatctc agttatatga cgtgacatta tattatcgca
     3061 aagacttgag tgatcgtcgt caattgtcag atgatatcac gatgatagaa attgctgatt
     3121 atgaacaaga tgttttagaa cgactaggtc tgtttgaccg agacatagtt gtttgtgcta
     3181 cgaatgacga tgatattaac cgaaaagttg ctaaattagc caaagcacat caagttgagc
     3241 gtgtcatttg cagacttgaa agcacaacgg acgatacaga gttagttgat tcaggtattg
     3301 aaattttcag tagctactta agtaataaaa tcttattaaa aggtttaatt gaaacaccta
     3361 acatgttgaa tttattaagt aatgttgaaa cgtcactata tgaaattcaa atgttaaatt
     3421 ataaatatga aaatattcaa ttacgtaatt tcccattcgg aggagacatc atcttcgtgc
     3481 gtattatccg taataatgag tcgattgttc cgcatggaga tacacaattg cgatatggag
     3541 atcgcttaat tgttaccggt gctaaagaat acgttgatga attgaagcaa gagttagaat
     3601 tttattttta acaataatga tattaaaatg cagacgtatt ttagtacgac gtaaaattaa
     3661 tgattttaaa atgctagtat gtatatgatt ttgataaata aatgcttttt acgtaaatca
     3721 agtttgatac agaaa
//
LOCUS       DAKTGS010000183         3674 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.188, whole genome shotgun sequence.
ACCESSION   DAKTGS010000183 DAKTGS010000000
VERSION     DAKTGS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.188"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..201)
                     /locus_tag="O0286_002646"
     CDS             complement(<1..201)
                     /locus_tag="O0286_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170980.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLIT"
     gene            complement(220..1896)
                     /gene="kdpA"
                     /locus_tag="O0286_002647"
     CDS             complement(220..1896)
                     /gene="kdpA"
                     /locus_tag="O0286_002647"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCY0170981.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(1920..2030)
                     /gene="kdpF"
                     /locus_tag="O0286_002648"
     CDS             complement(1920..2030)
                     /gene="kdpF"
                     /locus_tag="O0286_002648"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCY0170982.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            2168..>3674
                     /locus_tag="O0286_002649"
     CDS             2168..>3674
                     /locus_tag="O0286_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCY0170983.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTD"
BASE COUNT         1345 a          637 c          541 g         1151 t
ORIGIN      
        1 tgtaatcaaa taacctcgcg atagatagct agttccaaat atgtcaggaa aacatattaa
       61 tatcaacgtt aaaatcattc caacctcaac gacaaacatt atcggatttt ttattaattg
      121 tttaagattc agcttataaa aactcatttt caaagcttct atcaccattg tttgattgaa
      181 atatttattt acatgatgca ttttttccat ccctttactt tattttaaag ttaaaaattc
      241 accaatagga ccaagtaata gtactggaat aaatgtcaaa ccacttagta aaacgataaa
      301 tacgattagt gatacgccaa aataaggttt atcaatcgct attgtatatt tatcttgatg
      361 gtatgatttt ttattcacta aacttgatgc aatcattaat tgcaaaataa ttggtatata
      421 acgagaaagc aacataatga ttcctgtaga gatattccag aatgttgtat catctttcag
      481 tccttcaaac cctgatccat tgttcgcagc agctgatgtc atttcataca taacttgtga
      541 aataccatga aaagacggat tcgttatact ttcacttgct ccaggaatca taaaagcaag
      601 tgctgaaaat actaaaatta aaattgggtg tatgagaaag actaagacaa tacatttcat
      661 ttcacgggcg ccaattggca tatttaaata ttctggtgtt ttaccaacca tcaaactgca
      721 tataaacacc gtcagtaaga caaatatcaa taaattcatg agtcctacgc cttcgccacc
      781 aaatacaaca tttagcatca ttaataccat tggtcctaat ccacctatag gcgttaagct
      841 atcatgcatg ttattaacag aacccgttgt aaatgccgtc gtaataactg taaatagtgc
      901 tgacaaacct gctccaaacc gtacctcttt accttccata ttcggtccat aaatgcctaa
      961 attcgctagt attggattac cacgatactc actccacata gttaatgtaa gaattgctat
     1021 aaaaatgaaa aacattgcga caaataatat caacgcatga cgatgtactc gtttaccatg
     1081 tctacttaac atgcgaccaa ataagaacaa cattgacata ggaagtaaca tcatactgcc
     1141 catttctata aaattgctcc aaatatttgg attttcaaaa ggtgtcgcag aatttcctgc
     1201 taaaaatcct ccaccattcg taccaagatg ttttattgat tcaagtgatg caataggtcc
     1261 aaatgcaata tgttgaatat gtccgcttaa agtccgaatc attaaattag catgcaacgt
     1321 ttgtggtaca ccttgagtca tcaataaaat actaattaaa catgataatg gtaaaagtac
     1381 tcggacaata aaccgaacaa tatcttgata aaaattacca atgatattag ttaatccagt
     1441 taaacgtctc aacatcgcta tacaaacggc gtaacctgat gcactagatg taaacattaa
     1501 atatgtcatt acaatcattt gcgttaaata tgtcacatct gattcaccgt tatagtgttg
     1561 taaattacta tttgttaaaa aagatattgc tgtattaaac gctaaatcta tcgattggtt
     1621 taaattatga tttggattta aaaaaagcca ttgctgaact attagcaata caaatgttat
     1681 aaaccccata aatccattaa atgccagaaa atgtttgaca tatgttttag ctgacatgtg
     1741 ttctaaatct gtgccgataa ttttaaaaca catattttca aatctagtaa atattaaatc
     1801 tactcttgac gattgcacca atgctacgcg atatagatat ccactaaaaa catacgtaat
     1861 cataaccatc attgttagaa acaaaattat ttccatgata accctcactt aatatatttc
     1921 taaaattttt cactacgaat taaggcataa aataaataca aaactaatgc aataactacc
     1981 agtaataaaa cgatgagcat tgccataacc tccttacaac acaacaacat cgtaacaact
     2041 tgtttatgag agaaatatta attttcaaac ttagttatta agaaatcatt aagatgtgta
     2101 tgcagaaata aattttatag catttaattg tgaagaatat tatgatattg ctatcgaggt
     2161 gaaggttatg tcaaacactg aatcgctaaa cataggaaaa aagcgtggat ctttaacaat
     2221 ttacatcggt tacagtccag gtgttggtaa aacatttgag atgctttcaa atgccattga
     2281 actatttcaa agtaacgttg atattaaaat aggatatatt gaaccgcatc agcgtgatga
     2341 aacaaatgca ttagctgaac aattacctaa aatcacaacc aattttacta aacatggtag
     2401 tcatcatttt caatatttag atgtcgaccg cataatcgaa gaatcgccga caatagtact
     2461 tatcgatgag ttagcacata cgaatatttc tagagatcgt catgagaaac gatatatgga
     2521 tattgaagaa attttaaatc atggtatcga tgttcatacc actttgaaca ttcaacatat
     2581 tgaaagttta agtagtcaaa ttgaactgat gaccggtgta catgttaaag aacgtgtacc
     2641 cgactatttc ataatgagcg ccgatgtatt agaagtcgta gatatctcac ctgaacaatt
     2701 aattaaacgc ttaaaagctg gcaaggtata taaaaaggat aggctagatg tagcatttag
     2761 taatttcttt acgtatgccc acctaagcga actgcgtaca ttgacgttaa gaacagttgc
     2821 cgacttgatg agtgataaag aaaaagtccg acacaaccat aaaacgtcac tcaaacctca
     2881 tattgctgtg gcaattagtg ggagcattta taatgaagca gtaattaaag aggcattcca
     2941 tattgctcaa aaagaacatg cgaagttcac tgctatttat atagatgtat tcgaaaaaaa
     3001 caggcaatat aaagatagtc aaaagcaagt gcatcaacat ctcatgcttg caaaatcatt
     3061 aggagcaaaa gtaaaagtag tttatagcca aaccgttgca ttaggattag acgaatggtg
     3121 taaaaatcaa gatgtaacca aattaattat cggacaacat attagaaata agtggcgaga
     3181 ctttttcaat acacctttaa ttgaccattt aatgtccttt gaacatagct ataaaatcga
     3241 aatcgttcca atcaaacaaa tacctgttga attgaaaatg aacaaatcac cctatcgtcc
     3301 taaaggcaaa cgtttcgcca tagatatgtt aaaaatgatt ttgattcaaa taatttgtgt
     3361 aatgatggga ctgtggattt atcaacttga taagcatgag tctagtacga ttattttaat
     3421 gatttttctc atcggcatca ttttattatc catttggacg cggtccttca tcattggctt
     3481 tttagcagca attattaacg tatttgtttt taattatttt tttacggaac ctagatatac
     3541 atttgaagta tatcgctttg actatcctat tacatttatc gttagcattt taacaagtat
     3601 tttaacgagt gctttattaa aacaaattaa attccaatac tccattacta aaaagcaact
     3661 ttatcgtaca gatt
//
LOCUS       DAKTGS010000184         3632 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.189, whole genome shotgun sequence.
ACCESSION   DAKTGS010000184 DAKTGS010000000
VERSION     DAKTGS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.189"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(1..1289)
                     /gene="cstB"
                     /locus_tag="O0286_002650"
                     /pseudo
     CDS             complement(1..1289)
                     /gene="cstB"
                     /locus_tag="O0286_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            1345..1482
                     /locus_tag="O0286_002651"
     CDS             1345..1482
                     /locus_tag="O0286_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170984.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            1479..1850
                     /locus_tag="O0286_002652"
     CDS             1479..1850
                     /locus_tag="O0286_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0170985.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            1979..2599
                     /locus_tag="O0286_002653"
     CDS             1979..2599
                     /locus_tag="O0286_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170986.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
     gene            complement(3119..3307)
                     /locus_tag="O0286_002654"
                     /pseudo
     CDS             complement(3119..3307)
                     /locus_tag="O0286_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1278 a          482 c          580 g         1292 t
ORIGIN      
        1 gtttacatta ataatgttgt gataaccttt gtgttctaaa ataccaatag ctatagaact
       61 tctaatgcca gactgacagt ctacataaat aacatcgttt ctattgaaag gtaaatctgt
      121 ttctaaaagt ttgccgtgtg gcacatgaac cgcttgggat aagtggccat tattccattc
      181 attatcatta cgtacatcta agacatgtgc ttcattacca gttatgtctt tactatgaac
      241 agattgtgtt tgaatttgag cttgtggtaa ctgatatcca gacacattat catatccaat
      301 aagttgtaaa gtatgttatg tgttgctttt gaaacaaggt gatagtctcc aatcaagtta
      361 atttcttgat tatagtttag ataccagcca atttgattga tgaaattttt atcatatgga
      421 atattgattg taccttcaat atgtccacca tgataagcct ccttactgcg gagatcaaaa
      481 gttaatctgt ttgtacttgt agctggataa accgtataag gttgatataa attcataccg
      541 aattgattaa tttttttcat ttgtgcaaaa tgatgtggtg gtgcaggttg gtcagaaatg
      601 agtttatcga taaaggtagc ttcattattt tcagaaaaag cccagttcgt ttgtttttca
      661 tagccaagcg tagatgttgg aatagcacct aaagatttac cacaaggact accagcgcca
      721 tgaccaggcc aaatttgaat gtagtctggc aagtctttaa tactttcaat agatttaaac
      781 atttgttttg cgcctatttc agataatcct tctactttaa cagctttttc tagtaaatca
      841 ggtctaccga tatctcctac aaaaataaaa tcaccactga atagtcccat tggaacttgt
      901 gctccagcac cttcgtcagt aagtaaaaaa cttatacttt ctggcgtgtg accaggtgta
      961 tgaagcactt ttaattttat atttcctaca taaatatcat cattatgttg aacaaaatga
     1021 gtgtggttag gcatattttt ataacctaac atgtcatcac tttcgcccga tacataaata
     1081 ctagcattta actttatagc aacatctcta attcctgaaa caaaatttgc atgtatatgt
     1141 gtttcagctg catgagtaat ggttaaactc tcttcatcgg caactcgaat atatgaagat
     1201 aagtcacaaa taggatcaat gatcatggct tctccagttt tttgacaacc gattaaataa
     1261 gatgcttgag ataaatgttt atcataaatt gatgatagtc aatttgttat caagggtgat
     1321 aattatatta actatggtaa ttacatggaa ttaaagttat taaacaaact tgatgatgac
     1381 caattagatt tagaattttt acaagataat gggttaaaag gtaatgttca aggtcctatg
     1441 actttaaagg aacctttaaa aagttatata caaaagtctt gaaaacgaat attttaaatt
     1501 tagataatga gttagaaaac actgaatatg tcgaaggtaa accatatgta cagtacggta
     1561 ttaaatatga aagaaatcag gcattaagaa atgaagctat taaaattcat ggaactacat
     1621 gtaaagtatg tggatttgat tttaaagcta agtatggcga tttaggtgag ggttttattg
     1681 aaattcatca tttaaaacca atgttttcaa taaaaagaga aataaaagta aatccacaaa
     1741 aagatttagt cccactatgt tctaattgcc ataaaatgat tcatagaaat actaaaaaac
     1801 ctttaacgat taaagaatta accaaaatag ttaattataa tagcaaataa tttaatattt
     1861 tataaactat cattcaaccc tcttaattta ttaggaggtt ttttgtattt atgctttcaa
     1921 atgtgtgata tactttgttt gttgaaatat agagtatcta tagatagggt gattgagtat
     1981 gaaattcact gaagtggaag ttatcgaaca tcttgtaaag gcatataaag aagcaggaaa
     2041 gcctacttat cctcatgaaa atttatatcg aggacgtaat catagtattt caggtattgg
     2101 agaagacttg ctgggtgctt atttgattag tagattggaa ggtgtccaaa tatttattga
     2161 tcagccttta tctatgattg ataaatcttt aagtacaaga tatccggatt tattaatttg
     2221 tgaagataat gaaattaaaa atatactaga agttaaaatg gacttaggat atcaaagaaa
     2281 agattttata gattattgcc gaaagaaaga agaatggatt tcaaatatcg taggaaaaca
     2341 gtgtgtattg tctagaaaga gagaagacaa aattcctatg aatatagctg atgatattaa
     2401 atttcatgtt gtgatttaca gtgaaaacaa tggaccgaag cggtttgatg aagaaatcat
     2461 gcctatcgtt aatgaaacat gtccacatat tgaagtatat gtcctaacaa gcggtcaaca
     2521 ccctaattta gtaaatgtta atcttgaagg tattaatatt aataaagatg aatttgaaat
     2581 attagtaaat gcgttataaa aaaatagagc atcctccacg ttatggaggt gctctgtttt
     2641 ttattgaaaa gtatcaagtt aattaattta atatgcttaa taagttctac cttgaccttt
     2701 ttctctagct tctgttcgat ctcttatgta ctcagtacat actggatttt ctgttaattc
     2761 atcaactgta ttagtttaat cagaagacgt gtctactttg taagcttcta agaacttatt
     2821 atatgatata gcgtttgagt tttgttgttc ttctataaat ttctgtagtt atttttcaaa
     2881 aaccgcatca ttaactgata agcagaagcg tatcacaaat aaaactaaaa aatagattgt
     2941 gtataatata aaaggaaggg atttatatta aaattttgaa ttcaaaaatt attgaaaggg
     3001 aagctacctt agaaattgaa tctatggcca ctaactgctt ttttatatca ccacttatta
     3061 aaaaatgtaa gaatattcct cagtgctaag tgaaggaggt tctagtttta tttaatattc
     3121 atattgatag gtaatggtta ataattgcct gtaaattatt gttaaatatc gttgttatag
     3181 tatcatcatt cataaaattt ttatagattt tatcaagtat ttcctcatct tcaatagctg
     3241 taaagcgatg tactagtctt tttatgagct atatataatt ttgcgaagct aaactgtttg
     3301 taattattca ccaatcaggt tatgtagtgt cacggttcat aggtaattct ctttttaaaa
     3361 tcatctaata ttttatataa ttcatataag tgtagtatta acgttttcta ctttatctat
     3421 aaaatattta gaattacatt agaattttaa attatacggc tggaaattca atgctgtaaa
     3481 aatagacaga ttttaaaaaa gtagtaataa aaagtctaag ttttatcttt gtaataaact
     3541 tcgactatat ttaaaaataa atatgaatgt cttttaattt ctataatatc aaagctatta
     3601 tatgatttga aattttgtaa ctttttctct aa
//
LOCUS       DAKTGS010000185         3587 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.190, whole genome shotgun sequence.
ACCESSION   DAKTGS010000185 DAKTGS010000000
VERSION     DAKTGS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.190"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            50..700
                     /locus_tag="O0286_002655"
     CDS             50..700
                     /locus_tag="O0286_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCY0170987.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            781..1776
                     /locus_tag="O0286_002656"
     CDS             781..1776
                     /locus_tag="O0286_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCY0170988.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            1852..2478
                     /locus_tag="O0286_002657"
                     /pseudo
     CDS             1852..2478
                     /locus_tag="O0286_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2519..2863
                     /locus_tag="O0286_002658"
     CDS             2519..2863
                     /locus_tag="O0286_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCY0170989.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            2961..3512
                     /locus_tag="O0286_002659"
     CDS             2961..3512
                     /locus_tag="O0286_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170990.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
BASE COUNT         1459 a          568 c          603 g          957 t
ORIGIN      
        1 acacttaata actccctctt aaatgcatcc aggttttatg tagtaaatca tgaaaataac
       61 atataaatat agaggagatt tacctttgaa tacagagaac aacaagaatc aaaaccaatc
      121 tgttaaaaat tctgaaagac gcggcatgtt aaaaggatgc ggcggttgcc ttatttcttt
      181 tattttatta ataatcttat tatcagcctg ttcaatgatg tttagtaata atgacaattc
      241 cactaataat caatcatcaa aaacgcaatt aactcaaaaa gatgaaaata aaaatgaaga
      301 taagcctgag gaaaaatcag aaacagcaac agatgaggat ttacaatcaa ccgaagaagt
      361 acctgcaaat gaaaatactg aaaataatca acatgaaatt gatgaaataa caacaaaaga
      421 tcaatcagac gatgatatta acacaccaaa cgttgcagaa gataaatcac aagacgactt
      481 gaaagatgat ttaaaagaaa agcaacaatc aagtaaccat catcaatcca cgcaacctaa
      541 gacctcacca tcaactgaaa caaacacgca acaatcattt gctaattgta agcaacttag
      601 acaagtatat ccgaatggtg tcactgccga tcatccagca tatcgaccac atttagatag
      661 agataaagat aaacgtgcat gtgaacctga taaatattaa acaacaagcg aattgaattc
      721 aaattgtatt tagctttatg cactaatcac atagtaaata atgagggaga ttttttaggc
      781 atgagcaatc aattcaaaag cgaagaagag cgaagacaat gggaacaatt ccaagctttc
      841 caaaatcaac aaaaccaaca gaaccagcaa tacggacaaa agaaatctaa aaaaggatgg
      901 ttctggggct gtggtggttg tctagtatta tttattttaa ttatcatcgg tatttcagct
      961 tgtacagctg gtattacagg taaccttggc ggaaatagtt ctaaagaaac gaacaaaacc
     1021 cataaaatcg gtgaaactgt taaaaatggc gaccttgaag tcactgtaaa ttcagtggaa
     1081 actatgaaat ctgtaggacc atctcttgca ccaacaaacg ctaaaggtat atttgtcgtt
     1141 gctgatgtga cgattaaaaa caaaggtaaa gaagcgttaa caattgatag ttcaatgttt
     1201 aagctaaaat ccggtgataa aacatttgaa gcagataata caggttcaat gtctgctaat
     1261 caaagtgaca atggtagtat agaaaattca tttttcttac agcgtataaa tccagatagc
     1321 actgctcaag gtaaaattgt tttcgatgtg tcagaaaaca tagccaacgc aaaagataaa
     1381 aaattagaag ttatttctag tttatttagc gtcaagaaga ttacatttga tttatccgat
     1441 gctaaaaaaa catcaaaagc taaaaaagac aagcaagata cagaagtagc tgttgcgagt
     1501 tcaaatagcg ataatgtaag ttatgaagct tcggctacta cacctgctac aacttctagt
     1561 gcggatactg attctgaaga tagcgaaaag tctagtaaag atgaggataa gcagaatgcg
     1621 tctaaaagtg ataaatctag tgtagaaaaa agtgaatcta atgaggaaac tgctcctgta
     1681 gagcccatgc cccatagcaa acctaccact agtgaagcac cacctagcca aaatattcac
     1741 aatgaagata gcatgtacga cgcttcaaca gaataaaatt tcagggtagc tcggctaccc
     1801 ttcttttacg gaaaaattaa ttatacataa tcaaatcaag gagataaaaa aatgaaattc
     1861 aaagctatcg ttgcaatcac attatcattg tcactattaa ctgcctgtgg tgctaatcaa
     1921 cataaagaaa atagtagtaa atcaaatgac actaataaaa agacgcaaca aactgacaac
     1981 actacacagt caaatacaga aaagtaaatg acaccacaag aagccgaaga tatagttcga
     2041 aacgattaca aagcaagagg cgttaatgaa tatcaaacat taaattataa aacaaatctt
     2101 gaacgaagca atgaacatga atattatgtt gaacatctag tccgcgatgc agttggcaca
     2161 ccattaaaac gttgtgctat tgttaatcga cacaatggca caattattaa tatttttgat
     2221 gatatgtcag aaaaagacaa agaagaattt gaagcattta aaaagagaag ccctaaatac
     2281 aatccaggta tgaacaatca tgatgaaaca gatggtgagt cagaagacat tcaacatcat
     2341 gacattgata ataacaaagc aattcaaaat gacataccag atcaaaaagt cgacgataaa
     2401 aatgataaaa atgctgttaa taaagaagaa aaacatgata atggggcaaa taattctgaa
     2461 gaaactaaag ttaaataatg gcatactttg attaatcgta atttttagga gaaacgctat
     2521 gataacattt gagaatatac aacaacttga gaaatacacc ttaatgacta tgcatggtct
     2581 ttttaatcaa cttaaactcg gcattatttc aatcgaaaac gcagagcata cgctttttac
     2641 accttatatg ttggaaacgc tctcttccct aggcgtgaaa gacagcattg tcgatttaat
     2701 tcataaaggg actgaattag aagactttgc ggcatttaat ttatcaattg aagacacagt
     2761 tacagtctgt ttacaaagaa ctgaagaact attaaaacaa tacaaaaatg tggaattcaa
     2821 tgacaaaata ttaatcaatt ggcgtattat acaagagaaa tagacatata aaagtcgaat
     2881 gtaactacgt gagtattgat tttattcttt gtaaattaca agcatttcat attataaagt
     2941 ttgaaaagag gtatattgaa atggagaaaa atgaatatat agctaaatat aatgaatata
     3001 gtcaattatt agacgctaca tactcgcaag ctgtagcata ccttttaagt aaatatggcg
     3061 ctgtaaccga tgattattat aaagaaaaat catacacgcg atttttaaat ggagaaatca
     3121 aaagtatttc aaaaggaaaa tacactagag ctagcgaagg attatattgc catcatataa
     3181 gcgaggacaa attccaaaat ctatctgatc taagattcat ttccaaattt aagtactcat
     3241 acgacgttca aaagaaagaa aacttagtgt actgtgatct aatcgagcat ttaattttac
     3301 atgcaattat tacaaaagaa tcccatggcc aatttggtgt agctggatta tgtcaaatga
     3361 tcaaaccaac agtcattgat tggtacattg gcgaatataa tccaaaacca gcatggatgc
     3421 aagccaccaa agcacgtgcc tatttgcctg gaatattagt agagaaatta ctcattaaaa
     3481 ttgacgatat gttaaaagga atagaaatat aagatttcct tgagtctaga taaatgatta
     3541 atgtagattt attttttgct gttgagattt tgttatagat gtttaaa
//
LOCUS       DAKTGS010000186         3574 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.191, whole genome shotgun sequence.
ACCESSION   DAKTGS010000186 DAKTGS010000000
VERSION     DAKTGS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.191"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..297
                     /locus_tag="O0286_002660"
     CDS             <1..297
                     /locus_tag="O0286_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0170991.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="O0286_002661"
                     /pseudo
     CDS             355..732
                     /locus_tag="O0286_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="O0286_002662"
                     /pseudo
     CDS             729..1610
                     /locus_tag="O0286_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="O0286_002663"
     CDS             1726..2655
                     /locus_tag="O0286_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0170992.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="O0286_002664"
     CDS             2648..3385
                     /locus_tag="O0286_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0170993.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..>3574
                     /locus_tag="O0286_002665"
     CDS             3399..>3574
                     /locus_tag="O0286_002665"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="BacA; phosphatase activity in Escherichia coli not
                     kinase; involved in bacitracin resistance as bacitracin
                     supposedly sequesters undecaprenyl disphosphate which
                     reduces the pool of lipid carrier available to the cell;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HCY0170994.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLG"
BASE COUNT         1308 a          491 c          632 g         1143 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt tagg
//
LOCUS       DAKTGS010000187         3515 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.192, whole genome shotgun sequence.
ACCESSION   DAKTGS010000187 DAKTGS010000000
VERSION     DAKTGS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.192"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(38..250)
                     /locus_tag="O0286_002666"
     CDS             complement(38..250)
                     /locus_tag="O0286_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170995.1"
                     /translation="MNQFKEIYNTIEKLLNDKSISNYRINQDTDVSYGGISELRSGKR
                     KVNNLTLETAEKLYNYQKQLEIMIED"
     gene            complement(951..1148)
                     /locus_tag="O0286_002667"
     CDS             complement(951..1148)
                     /locus_tag="O0286_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0170996.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(1641..2981)
                     /gene="glnA"
                     /locus_tag="O0286_002668"
     CDS             complement(1641..2981)
                     /gene="glnA"
                     /locus_tag="O0286_002668"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCY0170997.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(3000..3368)
                     /locus_tag="O0286_002669"
     CDS             complement(3000..3368)
                     /locus_tag="O0286_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0170998.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
BASE COUNT         1087 a          622 c          462 g         1344 t
ORIGIN      
        1 ggctcactcc atgatatttc aaaaatatgg ccttatttta atcttcaatc attatttcta
       61 attgtttttg gtaattatag agtttttccg ctgtttctaa agttaaatta ttcacttttc
      121 ttttcccgct tcttaattca cttataccac cataagaaac atcagtgtct tgattaattc
      181 tataattaga tattgattta tcatttagta atttttctat tgtattataa atttctttaa
      241 actggttcat aatttttgtt aaaatgaaag aataatttat ttttttctag ttaaattaat
      301 aataaataat attaaagttg caatgttaag atagagagtt aattttttcc ttaattctct
      361 tgtcataaat accacctccc tatttaatgt ggtaaaggtc ttttgccctt taccttttta
      421 aaagaacgat ttgataaaat taagtacttg aattattaag ctcgtcacag ctattcgaaa
      481 tttaattttg ctattcgttc tttttcttta accattctta tcattttttc tcctttcctc
      541 tttatagtta tttaacattc cacaaattgg ggtaagtgac atgttgaact cccatatcca
      601 ctggcaagat ttataatttg taattctaaa gtataatagc ctatattttt caaaatctct
      661 ttttttatga ttaacatccc tctaagtgca cctaaaggtt tttatgtaat gtattaaata
      721 tttccattta tacatataca tcttgtataa aaagaaagaa ctccatatat actcaaaagg
      781 tatactgaag ctctatatat tatataaaat tattatacta ttttgaaaaa cataagtaaa
      841 tcactaaaca tgatttcttt cacttttaga aaacttttaa tactataaaa gcacccactc
      901 agtcactagt ttgggcagtt attgtatgcc tattgaactc aatgcttata ttacaatacc
      961 tttttcgcat attcatataa gatctttgca ttctttaagc ttaaattttc tattcttctt
     1021 tctctacggc gcatagcatt aatattaccg taacttatcc cagtatcttt attaatttga
     1081 taactcgata tctctttgtt ttctatcaat tctttgattg tattgaatat ttcatcatag
     1141 caattcataa attagatgag gcgaaatttt taatttttta gaatatcaat agtagtataa
     1201 ctaaaatgaa aataccgatc gataaacaaa aagatatttt ttgttttgtt tctcttttca
     1261 tatagtatta cccccttaat aatgcgtagt aaggtccctc ttttcggggt cttaccttag
     1321 aacgttctgc aaatgaattc gatgagaagt aatatgaata tggctatttt caagtaatac
     1381 tcaacgtttt cgcgacgttc ttttatcgcc tcatctcatc acctccaaat atattaaaat
     1441 tcatgtgaac taaaatataa aatggtcttc cccagcttta aaaaaataaa tacataaaac
     1501 atttacttga ccaaaacttg accaaatcca ttttttaaca aaagtggtca agtgaaatcc
     1561 catctttcca aatcaaaaaa gagctaaagc aaaatgctct agcccttgat attactgatt
     1621 tcccatcatt gtttagcgta ttaatattgc ttcatgtact gatctctttc ccattcagag
     1681 acttgagttc tgtagtaatc ccattcaatt gattttgaat taataaattg attatagata
     1741 tgatttccta aagctttttt aataacttca ttttcacgca ttgcttttaa tgcagtataa
     1801 agtgttgaag gtaagtcttg aatgcctact gcttcacgtt cttcacggtt catttcgtaa
     1861 atattttggt taactggttc tggaactttt aatttatttt taataccatc tagtccagct
     1921 tctaagattg cagctaacgc catgtatggg tttgcagctg gatctactga acgtacttcg
     1981 atacgagtag ataatcctct tgaagatggt acacggatta atggtgaacg gtttttacca
     2041 ctccatgcaa tataacatgg tgcttcataa ccaggtacta aacgtttata tgagtttact
     2101 aacgggttac atacagcagt aaatccgcgt gcatttttaa gcacacctgc tgtaaattga
     2161 tatgcagttt ccgttaagcc catttcagta tttggatcaa agaatgcatt ttctttacct
     2221 ttgaataatg aaacgttaaa gtgcataccg ctaccattca caccgaataa tggtttaggc
     2281 ataaatgttg cgtgtaaatt atgtttacgt gcgattgttt taacaaccaa tttaaatgtt
     2341 tggatattat cacatgctgt aacagcatct gcatatttaa agtcaatttc atgttgacca
     2401 ggggcaactt catggtgact agcttcaata tcgaagccca tatcctctaa ttctaaaaca
     2461 atatcacgac gacagttttc acctaaatct gtaggtgcta aatcgaaata tccaccatca
     2521 tcattaagtt ctaaagttgg ttcccctttt tcatccaact taaacaagaa gaattctggt
     2581 tcaggcccta ggttaaagtc tgtgaagcct aaatcttcca tttcttttaa tacacgtttt
     2641 aagtttgcac gaggatcccc ttcaaatggt gttccatctg ttttatatac atcacaaatt
     2701 aaacgtgcaa ctttaccttg tccagcagtc catgggaaga ttacccaagt atctaaatct
     2761 ggatgtaagt acatatctga ttcttcgata cgtacgaaac cttcgataga agaaccgtca
     2821 aacatcattt cgttatcaag tactttttct aattggctta caggcacttc aacattttta
     2881 attgttccta aaatatcagt gaattgtaat cttaaatatc ttacattttc ctcttctgca
     2941 aatttacgaa tgtcgtcttt agtgaaagta cgttttggca ttttaaaatc ctccaaattt
     3001 tatttaataa atcgggataa atcaccacga tttattggca aagtttctcc aataggcttt
     3061 tgcgtggcat ctacaatcat cttttttctt atctcttgtt catctgttgt taaatgctct
     3121 tgtgagtcat aaatgatttg tttaatccct ttgatattaa atcctttttc taataatgat
     3181 ttaatttcta gtaatctttc caaatcattg agtgagaaca gacgtttttg accttctgtt
     3241 ctttcaggtt tgatgagttc atgtgtttca tagtaacgta tttgccttgg cgttaaatcc
     3301 gttaacttac ttactacact catagagaag acagccatat ttcgtctgat tgcatcattc
     3361 gatatcattg ttcctctcct ctacttttga acttgttaat aacttatcat aattttttta
     3421 ggatttcaaa aatatgtcag gttttctaac ataaagctta aaacattgat ataactgcga
     3481 tttcgacttc taaaacgaca taaaacaatc tacag
//
LOCUS       DAKTGS010000188         3501 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.193, whole genome shotgun sequence.
ACCESSION   DAKTGS010000188 DAKTGS010000000
VERSION     DAKTGS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.193"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            140..775
                     /locus_tag="O0286_002670"
                     /pseudo
     CDS             140..775
                     /locus_tag="O0286_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1302
                     /gene="sat4"
                     /locus_tag="O0286_002671"
     CDS             772..1302
                     /gene="sat4"
                     /locus_tag="O0286_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0170999.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1395..2189
                     /locus_tag="O0286_002672"
                     /note="aph(3')-IIIa"
     CDS             1395..2189
                     /locus_tag="O0286_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0171000.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2465..3036
                     /locus_tag="O0286_002673"
                     /pseudo
     CDS             2465..3036
                     /locus_tag="O0286_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(3205..>3501)
                     /locus_tag="O0286_002674"
     CDS             complement(3205..>3501)
                     /locus_tag="O0286_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0171001.1"
                     /translation="PQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
BASE COUNT         1153 a          555 c          785 g         1008 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc
      961 cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc
     1021 gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc
     1081 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1141 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1201 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1261 gccaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     1321 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     1381 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     1441 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     1501 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     1561 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     1621 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     1681 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1741 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1801 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1861 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1921 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1981 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     2041 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     2101 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2161 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     2221 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     2281 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     2341 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     2401 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     2461 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaata
     2521 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     2581 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     2641 cttgtagagg gcttcctact tctgccggta atgtttacag cggcggaagt gggtgcattg
     2701 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     2761 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     2821 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     2881 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     2941 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     3001 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     3061 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     3121 tggaacaaat attaaaacaa atgctggttc tgttgcaaag taaaaaaata tagctaacca
     3181 ctaatttatc atgtcagtgt tcgcttaact tgctagcatg atgctaattt cgtggcatgg
     3241 cgaaaatccg tagatctgaa gagacctgcg gttcttttta tatagagcgt aaatacactc
     3301 aatacctttt aaagtattct ttgctgtatt gatactttga taccttgtct ttcttacttt
     3361 aatatgacgg tgatcttgct caatgaggtt attcagatat ttcgatgtac aatggcagtc
     3421 aggtttaagt ttaaaagctt taattacttt aaccattgct accttcgttg aaggtgcctg
     3481 atctgtaatt accttttgag g
//
LOCUS       DAKTGS010000189         3180 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.196, whole genome shotgun sequence.
ACCESSION   DAKTGS010000189 DAKTGS010000000
VERSION     DAKTGS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.196"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            69..248
                     /locus_tag="O0286_002675"
     CDS             69..248
                     /locus_tag="O0286_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171002.1"
                     /translation="MLLISRGVFDLNDFIRLKVFSSYNNIHDKILYLYRKFLDLLDLT
                     DNTFEKIVKSNYIEC"
     gene            303..653
                     /locus_tag="O0286_002676"
     CDS             303..653
                     /locus_tag="O0286_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCY0171003.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            646..1209
                     /locus_tag="O0286_002677"
     CDS             646..1209
                     /locus_tag="O0286_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCY0171004.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            1223..1567
                     /gene="gpsB"
                     /locus_tag="O0286_002678"
     CDS             1223..1567
                     /gene="gpsB"
                     /locus_tag="O0286_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCY0171005.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            1630..2009
                     /gene="rnpB"
                     /locus_tag="O0286_002679"
     ncRNA           1630..2009
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O0286_002679"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            2218..>3180
                     /locus_tag="O0286_002680"
     CDS             2218..>3180
                     /locus_tag="O0286_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCY0171006.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRY"
BASE COUNT         1174 a          423 c          616 g          967 t
ORIGIN      
        1 tttttgaatt ttgttataaa agtcaaatca tgattagtaa tttttatttt tgaggcaagt
       61 acaagtatgt gttgttaatc agcagaggtg tatttgattt aaatgatttt attagattaa
      121 aagtattttc atcgtataac aacatacacg ataaaatttt gtatttatat aggaagtttt
      181 tagatttact tgatttaaca gataacacat ttgaaaaaat agttaaatca aattatatag
      241 agtgttaaaa catgacgaat acagtatatt gtgataaatt aaaaaatgta ggaggcgtcc
      301 tcatgaatga tgtagtcgaa tcactaattt atgaagttaa caacatgcaa caaaattttg
      361 aaaatgtgaa atcacaacaa caagatcatg atttttacca aactgtaaag ccatatactg
      421 aacatattga tagcatactc aatgagatca aattacatcg tgaatttatt atagaagtac
      481 cttatatgaa ttcaaggaaa tttgagctac tgattgctaa cattgaacaa ctttctgtcg
      541 aatgtcattt taagcgaaca agtcgaaagt tatttataga aaagcttaaa agtgttcaat
      601 atgatttaca aaatatatta gatggcgtaa caaaagaggg tactgatggt taaaacagtt
      661 tatgtaacag gttacaaatc attcgaatta aacattttta aagatgacgc acctgaagta
      721 cattatttaa aacaatttat aaaacataaa attgaacaac tgttggatga aggattagaa
      781 tgggtgttaa tacaagggca aatgggtata gaattatgga ctgcagaagt tgttattgaa
      841 ttacaacgaa catatgattc gttaaagttt gctgtaatta caccgttcca aggacataca
      901 gaaaagtgga atgaacataa tcaaagtaag tatgccaaca taattaagca tgcagattat
      961 gttgatagta tttttcatac gtcgtatcaa ggtccttttc aatttaaaca agcagatcaa
     1021 tttatgcttg aacattcgga tcaaacattg ctcatttatg atgaggaaca agaggcgagt
     1081 cctaagttct tcaagcagat gttagttgat tttatggata aaacaaacta tacttgtgat
     1141 attgtgacgt tcgatgaatt aacagcattc atcaatgact tacagtggtc tgaagatcaa
     1201 agtttctaat gaggtggaaa aaatgtcaga tgtttcattg aaattatcag caaaagatat
     1261 ttatgaaaaa gattttgaaa aaacgatggc tcgtggctat agaagagaag aagtagatgc
     1321 atttttagat gacattattg ctgattatca aaaaatggcc gatatgaata atgaagttgt
     1381 aaaattatca gaagagaatc ataaacttaa aaaagaatta gaagaattaa gactacgtgt
     1441 agcaacatca agacctcagg acaataaaag tttttcttcg aataatacaa caacaaatac
     1501 atcttcaaat aatgtagata ttttaaaacg tatttcaaac ttagaaaaag ctgtatttgg
     1561 taaataatta ctaaatacaa aagtttaact gtcttactaa taatgactat gttataattt
     1621 taaaagtgat attttgggta atcgctatat tatatagagg aaagtccatg ctcacacagt
     1681 ctgagatgat tgtagtgttc gtgcttgatg aaacaataaa tcaaggcatt aatttgacgg
     1741 caatgaaata tcctaagtct ttcgatatgg atagagtaat ttgaaagtgc cacagtgacg
     1801 tagcttttat agaaatataa aaggtggaac gcggtaaacc cctcgagtga gcaatccaaa
     1861 tttggtagga gcacttgttt aacggaattc aacgtataaa cgagacacac ttcgcgaaat
     1921 gaagtggtgt agacagatgg ttatcacctg agtaccagtg tgactagtgc acgtgatgag
     1981 tacgatggaa cagaacatgg cttatagaaa tatcactact agtttagctc tcctagatga
     2041 tggagagctt ttttcatgaa aagaacactt aaaattaacg ccttgtcttg atataatgac
     2101 actgccttgt tttaaaatag taagcggatg cgttaatgta tcagcgatta aatttgttgg
     2161 aaatgtataa aaaacacaag ctaagaataa aatacctgta taaaaggaga atcatatatg
     2221 tttcaattac ttgcagtttg tccgatggga ttagaagctg ttgttgctag ggaaattcaa
     2281 gaattaggct atgaaacaaa tgttgaaaat ggtcgtatat tttttgaagg agacgcaagt
     2341 gcaattgtaa aggcaaattt atggttgcgc acagcagacc gaatcaaaat tgttgttgga
     2401 cgttttaacg caacaacgtt tgacgaatta ttcgaacaaa ccaaagcgct cccttgggaa
     2461 tctataattg ataaagaggg taacttccca gttcaaggta gaagcgttaa atcaacacta
     2521 catagtgtgc cagattgtca ggcgataact aagaaagcta ttgttgaacg attacgtcgc
     2581 gcatataacg aaaaaggttg gttaaatgaa tcaggtgcca aataccctgt tgaagttgcc
     2641 attttaaaag ataatgtatt attgactatc gacacatcag gttctggttt gaacagacgt
     2701 ggttatagat tagcacaagg tgaagcacca attaaagaaa cgttggcagc aagtttaatc
     2761 cgtcttgcaa actggaaagg tgatacacct ttaattgatc cattttgcgg ttcgggaaca
     2821 atagctatag aagcttgttt aattgctcaa aatatcgcac ctggttttaa tcgcgagttc
     2881 gtatcagagc aatggaacat catgccagca aatatttatg atgattaccg tgatgaagcg
     2941 gataagatgg ctgattatga taaagaaatc gaagtatatg cttctgatat cgatccagaa
     3001 atggtagaga ttgctaagcg taacgctgaa gaagttggat tgtctgatat tattaaattt
     3061 agtgtaaaag atgtcaatac attaacaatt gatacagaag aaccggtggc gttaattgga
     3121 aatcctccat atggtgaacg tattggtgat cgtgaagaag ttgaagaaat gtaccgttat
//
LOCUS       DAKTGS010000190         3125 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.197, whole genome shotgun sequence.
ACCESSION   DAKTGS010000190 DAKTGS010000000
VERSION     DAKTGS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.197"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(55..243)
                     /locus_tag="O0286_002681"
     CDS             complement(55..243)
                     /locus_tag="O0286_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171007.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKVMNFKKNN"
     gene            complement(826..1821)
                     /locus_tag="O0286_002682"
     CDS             complement(826..1821)
                     /locus_tag="O0286_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCY0171008.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADIL
                     EILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQK
                     VIEKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFM
                     RSTEVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVAN
                     VKAIKPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSY
                     GGLLKQKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            2273..2854
                     /locus_tag="O0286_002683"
     CDS             2273..2854
                     /locus_tag="O0286_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171009.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIA
                     DKNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDI
                     ENKKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVA
                     KKLAKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
BASE COUNT         1139 a          519 c          379 g         1088 t
ORIGIN      
        1 aaaaaaacag tgtgaaaaca agaagaaact cgtttcaaaa tcccctcaaa tattctaatt
       61 attctttttg aaattcataa ccttaacaac caaataaata gcatttattc ccatcaaaat
      121 aacatacgga attaatatat ataaattttc caaatgagat ataaaaatta atccaagtaa
      181 aaagaaaaca tttaaaatag ttgctataac gtaaccttca tctaacactg tgtttttatt
      241 caaaataaac actccttttt taaaaaactt tatcacaaga cttactcaaa ttataaatac
      301 ctttattttg agttttaagg gggtatttta aaggtttagg gggtatcata taggttttta
      361 acttaaaaat atatacaagc tcttaaaacg caaatatgag ccaaataaat atatggtaat
      421 ttcacaaaca aaaatttcag caaaaccagt gactaatttt ttaacactgc ccatttacat
      481 gcaaattaaa aattagcata aaatccttta aaacttccac atgtttttct aaattcctgc
      541 agggcgtttt ttatcaaaaa tcgagaattt ttaatttttc tctaaaaact ccgtaagagc
      601 ataaggagaa tttgctatca ttcaaaaatg ataggtctgg caaagccaga aattccaggg
      661 gttttaaggt attttaaagt gatatcatac aaagctgtac tgtcttattt ttgtgacaca
      721 atatattgcg tttgtcttat ttttgtgaca ctacatacag catttgtcac aaaaataaga
      781 cagtactttt ttataaaaaa tcaacaaaaa agacattagt caacactaat gtcttaaata
      841 ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt cttcttcgtc
      901 tgttgtttta tcttcatcac ttgcattaat caaattacca tcttcgacat cgtctaagtt
      961 taaaatttta tgtttttgtt taagcaatcc accataactt aacatacgtt ttcgatacaa
     1021 acctttttct aaatcactta caatttcttg atttttttca tcatcatcag ttaaaaaatc
     1081 agatgattta accgaatatt ttgaggtctc tttgattgcc gattcaatgt ctttatcgcc
     1141 ttttctattc ggtttgatcg ctttaacatt agcaacaggt cgatagtcaa cttgtaatgc
     1201 tctttgccat aaattaaccc attcttcttg agttatataa ttctcttttt ttctaaaata
     1261 tgcattttca acacataaca aaacatgcat gtgctgatta taactaccgt catttttatt
     1321 aacggtaact tctgttgaac gcataaatcc aacaagattt tgtttaacct ttttatatct
     1381 actcaaccta tcaaatgctt tagttagatg cttcaaactt tgttctaaag tatctccatc
     1441 tatcgcattt tttgttgaaa gtgttaaaaa caaccaacgt gcttttggct tttccttaat
     1501 tactttttca atcacttttt gagcttgata actatttttc atagcacgtc tccaattaca
     1561 aaccggacat aattttgatt tacaaaacca tgtcttatgt aacttcaaat agccttcatc
     1621 agttggctta aattctaaaa tattaccaca ttgttttaca ttaaaagcct ttttgatttt
     1681 taaaatttct agtatatctg catagcttac attatctatt ttcttttctc tccatgggcg
     1741 ttgtttccca cttttcgtca tatcttttaa cgttttatta tcttgatttt cggttatact
     1801 tctagtagta ttatattgca tataaaaaga cccccagtat tatttaagat tcgacacctt
     1861 aataatataa ctggggtttt ttattgtcaa attttccttt cacgcacaaa aacacgttta
     1921 aacactgtaa tatcaacagt tacagctata tatataaaat cgagttattt ctatatagta
     1981 tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa caacctattc cttcttcttt
     2041 ttatgaccct ctaaacctaa ataaattaat gtgaaaacca atgaataaat aaaactttct
     2101 aaaaagtcaa aaggattatc aaataaaatc gaattaacaa aattaaaaat taaaaataca
     2161 gataataaaa taatcaaaaa gtaaaaaaac ttattcatat taaactcctt tgtttttata
     2221 tgttaatatt atattgaatt catttaaata acaaaacaag ggggcattta caatgaatgc
     2281 aattcaaaac agtatatcac taagaaagat tttagtagta tctttaagta ttttaaccat
     2341 actttcaatg gtcttggatt ttaatttcaa agaagctcaa gcccaaaaca aaaataacat
     2401 tgcagataaa aacgttgaaa ctttaaacga aaaagaaatt gaaaaagaac taaaatacat
     2461 atatggcaaa attattattt tagataaaga cggaacagct aaagatgtta atctagaaaa
     2521 tgccaaatct cgatatggat atgttcctga aggattccaa aaactaaaaa acgacataga
     2581 gaataaaaag aaaactagtt caatatcacc tagagcagtt ggtgggaact acaaaaatag
     2641 cggtgactgt ttctattctg aagttttaaa ctcatatgga gaacttttaa ctggaaacat
     2701 aatcacagca gtttttgaag atgttaaagc caaaaactta aagggcgtag caaaaaaatt
     2761 agctaaaatt gggataaaag gaaacctagc tggaatcgca gctactatgg tttcgaagga
     2821 tgtacaatgt aatcttaaat acgggttact ttagatacta tttattccaa ttgctttatt
     2881 gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg gcttaatagc
     2941 tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa catcaataaa
     3001 ttttctcggc ataaatgcgt cgtctatttt ttattatctt catgataatc atatttttct
     3061 tccatctcgt caaaagcatc atctttatag aaaaacaaaa agtaagcaac aacaccaata
     3121 ggaat
//
LOCUS       DAKTGS010000191         3109 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.198, whole genome shotgun sequence.
ACCESSION   DAKTGS010000191 DAKTGS010000000
VERSION     DAKTGS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.198"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..1288)
                     /locus_tag="O0286_002684"
     CDS             complement(<1..1288)
                     /locus_tag="O0286_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0171010.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYT"
     gene            1449..2360
                     /locus_tag="O0286_002685"
     CDS             1449..2360
                     /locus_tag="O0286_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0171011.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            2422..>3109
                     /locus_tag="O0286_002686"
     CDS             2422..>3109
                     /locus_tag="O0286_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0171012.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILP"
BASE COUNT         1251 a          372 c          487 g          999 t
ORIGIN      
        1 tagtgtagcg gtaattttta ggactttttg gtggtataaa tgttcttaat aaagttaata
       61 gtcctacttt accgcaaagc ataacgaata taataattat tttagtaata ccatgatatt
      121 ctgtggtaag gttcatactt aaccctactg ttccgaatgc agaaaccact tcgaataata
      181 acttgattaa tgatatgttc ggattaatta tcgataatat aaaagtaatg atgctgataa
      241 atagaaatga gatattaatg gtaacaatag atagttttat atgtttgtca gatatttctt
      301 tattgaatac tgaaacatta ttttctttac gtatataatt tagtacaaaa ataaacgcaa
      361 ctgcaaaagt agttatttta attcctccag ctgcactgag aggggcacca ccaataaaca
      421 taagtagcat taacattaat gcggtagatt tgttaatgct tgctatatct atactgttaa
      481 aacccgctgt tcgtgttgtt actgattgga aaaaagaatt tccgattttt tcaactagtc
      541 ccatatgttg catagtatta aactgttcta ataaaaagaa tgtaatagct cctataatta
      601 ttaggatact agttgtagtt aagactaatt tagaatgtaa agataattta ctcaattttt
      661 tacaattaat aaagtctatc acgacaaaat gtccaatacc tccaaatatt atgagtattg
      721 agattgtaat aatgacaatt ggatcactag aataatctat taagttattc ttaaaaaggg
      781 caaatccagc attattaaaa gctgatactg atgtgaataa gcttaaaaat aaacctttgc
      841 ctataccaaa ttttggtata aaagataaac acaaacaaat cataccaatt aattcagtga
      901 ctaaactata aatagccaag tgtttaatta gcttaataac accaccaggt tcgtcaatat
      961 tccatgtaac cataatcaag aatctatttt tcattgatat ctttctattt aaaaatacta
     1021 gtgtcaatag ggttacggtc acgataccca gaccacctat ttgaattaat aatagtatta
     1081 ctatctcacc aagtatatta aactgtgatc ctatatcaac tggggacaag ccagtaactg
     1141 taaatgcact tgaagctata aatagggcat ctaaaaaaga tattggcttt ttaccagtaa
     1201 aaggtaaata caataaaagt gcacctatga tagttgtaga gaagaaaagc attaaataaa
     1261 aatataaagg tttgtggact ttgttcattt taatttcata ctcctttata ttataaatta
     1321 ataattagat aatatcataa atgaaaatag aaatgttact gatgtgtatt actaacaggc
     1381 gttgactaat tcttttaata ttttataatt gaaaatgaat atcaattgaa aaacaaaagg
     1441 atgatattat gaaaagatta attggaattt tattatgtaa tttatttatt ttgactgcgt
     1501 gttcagcatc tgtcgataaa acaagtaact cgacaaagac tacagactat aaaattgaaa
     1561 atggtgaaac actgaaagta ccagagaaac ctaaaagagt tgctgtatta actggatttt
     1621 atgttggtga ttttataaag ttaggaatca aaccaattgc tgtttcagat ataactaaag
     1681 attcttcaat tttaaaacct tatttaaaag gggttgatta tattggagaa aatgatgttg
     1741 aaagagttgc taaagcaaaa ccagatttaa ttgttgtaga tgctatggat aaaaatataa
     1801 aaaaatacca aaaaatagcg ccaacaatcc catatacata caataaatac aatcataaag
     1861 aaatattaaa agaaataggc aagttgacta ataatgaaga taaagctaaa aaatggattg
     1921 aagagtggga cgataaaact agaaaagata aaaaagaaat tcaaagtaaa attggtcaag
     1981 caacagcatc tgtgtttgaa ccagatgaaa agcaaatata catatataac tctacatggg
     2041 gtcgtggttt ggatattgtt catgatgcat tcggtatgcc aatgacaaag caatataaag
     2101 ataaattaca agaagataaa aaaggctatg cttccatttc aaaagaaaat attagtaaat
     2161 atgctggtga ttatatattt ttaagtaaac cttcgtacgg aaaatttgat tttgaaaaaa
     2221 cacatacatg gcagaatatt gaagctgtaa aaaaaggaca tgtaatttca tataaagcag
     2281 aagattattg gttcacagat cctattacat tagaacattt gagaagtaaa ttaaaaaaag
     2341 aaattttaaa taaaaaataa tagaaataag ttgtaaaaat tttcttatgc attggtacta
     2401 atgtttttaa ggagtgatta aatgaagcaa ctggttggaa ttcccgaatc aatgttaatt
     2461 cctttgatag ctcgagcaaa agagtacgaa aacgaaaaac caataataaa agacgcacta
     2521 tcaaaaaaaa tatttgatgg tttagatgat atgtacaaaa atgttacatg tgatgacatg
     2581 tctcaaattg gaattagtat acgtactgtg ataatagatt gtgttactaa gaggcttatc
     2641 aaggataata aagatttaat cgtggtcaat ataggttgtg gcttagatac aaggtttcaa
     2701 agatttaata aagaaaaaat atcatggata gatttagatg taccagaatc aatagaaata
     2761 cgaaaaacat tttttaaaga aagtaatagt tataagatga tatctaaatc tatgctagat
     2821 tacagttgga ttgatgatgt caaaaattat aaatttttta atagtaagtc aaatatattg
     2881 tttattattg aaggtgtatt gatgtatttt gatgagagtg taatgactca attattggac
     2941 actattatca aaaagatggg agatcataat ttgacatttg cgattgaatt ttgctcaaaa
     3001 acaattgcga ataatacaaa gagacatcaa tcggtatcaa agttatcctc acaacctgtt
     3061 tttaaatatg gttacaatga tttaaaaaaa ttgaatgaaa ttttaccaa
//
LOCUS       DAKTGS010000192         3064 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.199, whole genome shotgun sequence.
ACCESSION   DAKTGS010000192 DAKTGS010000000
VERSION     DAKTGS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.199"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            221..727
                     /locus_tag="O0286_002687"
     CDS             221..727
                     /locus_tag="O0286_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171013.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            986..1483
                     /gene="efb"
                     /locus_tag="O0286_002688"
     CDS             986..1483
                     /gene="efb"
                     /locus_tag="O0286_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCY0171014.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            1637..1987
                     /gene="scb"
                     /locus_tag="O0286_002689"
     CDS             1637..1987
                     /gene="scb"
                     /locus_tag="O0286_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCY0171015.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            complement(2640..2825)
                     /locus_tag="O0286_002690"
     CDS             complement(2640..2825)
                     /locus_tag="O0286_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171016.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
BASE COUNT         1164 a          385 c          475 g         1040 t
ORIGIN      
        1 tgattaatat ataagttaaa aattatacta tttaattgaa ttatgcattt tcgaagtaaa
       61 cataattcaa cacttacatt tttaaataga taaaatagtg taaaatcacg tgccaagatt
      121 actatagagt aggcattgag tattattggc aatcacttaa gtgattgtta tttttcgtgg
      181 tcaataataa agatataaat aaatatattg aggtcaaacg atgataatta aaattttaac
      241 aattctatta ctactttgta tattgagcta tttggttaca aatagaaaga agccttttct
      301 gttcttaaag acactcttta tgggtgtggt atttatcttt ataggatata tttcactggc
      361 aatatctgcc gtaattattt atggtattat tcaatttatc acaattgatt ttggtagttt
      421 tttcttaatg ggtattatat tgatcttgat ttcaagtata ttccaattat ttatagttag
      481 attacttttt agaaaaaaga atgtcgattt gacagaggtt gtcgttttag agcatttaat
      541 tcaatggttc ttagtttact ttgcgatcta tcaagcagta aatgaaaaaa tggacattaa
      601 tgatattaat atcgacaatt tccaatctgt cttttttgac gtgtctaatt tgaatttagt
      661 aattctacca acgttaatca ttagctgggt cacaatattt aactatagaa tgagaagtta
      721 caaataaaat ctatgagatt ataccttcag acaccaacat tcaaatggtg tcttttttgt
      781 tgtgtggttt tatttttgaa attcgaaaaa gtagaggcat gaattttttg actagtgtat
      841 aagtgctgat gagtcacaag atagatagct atattttgtc tatattataa agtgtttata
      901 gttaattaat aattagttaa tttcaaaagt tgtataaata ggataactta ataaatgtaa
      961 gataataatt tggaggataa ttaacatgaa aaataaattg atagcaaaat ctttattaac
     1021 attagcggca ataggtatta ctacaactac aattgcgtca acagcagatg cgagcgaagg
     1081 atacggtcca agagaaaaga aaccagtgag tattaatcac aatatcgtag agtacaatga
     1141 tggtactttt aaatatcaat ctagaccaaa atttaactca acacctaaat atattaaatt
     1201 caaacatgac tataatattt tagaatttaa cgatggtaca ttcgaatatg gtgcacgtcc
     1261 acaatttaat aaaccagcag cgaaaactga tgcaactatt aaaaaagaac aaaaattgat
     1321 tcaagctcaa aatcttgtga gagaatttga aaaaacacat actgtcagtg cacacagaaa
     1381 agcacaaaag gcagtcaact tagtttcgtt tgaatacaaa gtgaagaaaa tggtcttaca
     1441 agagcgaatt gataatgtat taaaacaagg attagttaaa taaaacttca atcgttgctg
     1501 ttatctggaa ataattaatt aaatgttatg ttaatttttg ttaatgaaaa aagtaatcta
     1561 tttaatgaca ggttaatgta attgtcctga aattgactat atactcagta agtatcaatt
     1621 ttaaggagag cttataatga aatttaaaaa atatatatta acaggaacat tagcattact
     1681 tttatcatca actgggatag caactataga agggaataaa gcagatgcaa gtagtctgga
     1741 caaatattta actgaaagtc agtttcatga taaacgcata gcagaagaat taagaacttt
     1801 acttaacaaa tcgaatgtat atgcattagc tgcaggaagc ttaaatccat attataaacg
     1861 tacgattatg atgaatgaat atagagctaa agcggcactt aagaaaaatg atttcgtatc
     1921 aatggctgat gctaaagttg cattagaaaa aatatacaaa gaaattgatg aaattataaa
     1981 tagataataa ataaaacagg ttgagacaaa aaatggtctt aacctgtttt caatttgcat
     2041 atgtgataaa ttctatatca aaatgcttat gtataatgaa tgacatttaa aagtagggga
     2101 gacaaatata aatacaatag ttcctaggat tactctcaaa ataactatat caattattta
     2161 ctttgctctc ctatttttta aaatatctac atgtttaaac aatcaaaagt gtacaatatt
     2221 aaattatcat ttccagttct agtgctatat tggtagtagt tgactaaatg aaaataagct
     2281 tataacaagt tttttcaata ctcgtggggc cacaacagag agaaatagga tcaccaattc
     2341 caacaaacaa tgcaagttgg cgggggcccc aatatagaag ctggcgaaaa gtcagcttac
     2401 aaaaatgtgc aagttggcgg ggccccaaca tagagaaatt ggaaccccaa tttctacaga
     2461 caatgcaagt tggggtagga catcgataaa gaaatacttt ttctttagca attagtattt
     2521 cttatgcatg agctttactc atgtattcat tttttaagta cacattagct acagctaatg
     2581 ataaagaacc actacataat aaatcattag tggttcttta tcatttctat ctcactcttt
     2641 tactggaaga aaaagtttac gtttgtagaa catgccacaa taccaaaaat aattaagaaa
     2701 aataagacga taagcatgat gacacttttc aaacaacctc tatcagtttc tttcgatttt
     2761 ctttgttgaa cctttttata atcttcaagt agttttgcgg cttttttatt tatatgttta
     2821 ttcatgatgt tgactcctta taatatatgt ttaattcatt aaaatagttg aaaacatgac
     2881 ttgaaataaa gatataaagc taaagtaagg atgttcaatt gtttcaaatt ttatgaaagt
     2941 ataacgttaa atatgttaat ttgatgatga tatttgcttt ttattttcca aatggaattt
     3001 acttaaactg atgcattaaa atattaatga agcactagaa tacataaatg aatagtaatg
     3061 gtgc
//
LOCUS       DAKTGS010000193         3021 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.200, whole genome shotgun sequence.
ACCESSION   DAKTGS010000193 DAKTGS010000000
VERSION     DAKTGS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.200"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            15..2858
                     /gene="sdrC"
                     /locus_tag="O0286_002691"
     CDS             15..2858
                     /gene="sdrC"
                     /locus_tag="O0286_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCY0171017.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQ
                     VAYPDNSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETG
                     SENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1205 a          494 c          544 g          778 t
ORIGIN      
        1 aggagtatta ttaaatgaat aataaaaaga cagcaacaaa tagaaaaggc atgataccaa
       61 atcgattaaa caaattttcg ataagaaagt attctgtagg tactgcttca attttagtag
      121 ggacaacatt gatttttggg ttaagtggtc atgaagctaa agcggcagaa catacgaatg
      181 gagaattaaa tcaatcaaaa aatgaaacga cagccccaag tgagaataaa acaactaaaa
      241 aagttgatag tcgtcaacta aaagacaata cgcaaactgc aactgcagat cagcctaaag
      301 tgacaatgag tgatagtgca acagttaaag aaactagtag taacatgcaa tcaccacaaa
      361 acgctacagc taatcaatct actacaaaaa ctagcaatgt aacaacaaat gataaatcat
      421 caactacata tagtaatgaa actgataaaa gtaatttaac acaagcaaaa gatgtttcaa
      481 ctacacctaa aacaacgact attaaaccaa gaactttaaa tcgcatggca gtgaatactg
      541 ttgcagctcc acaacaagga acaaatgtta atgataaagt acatttttca aatattgaca
      601 ttgcgattga taaaggacat gttaatcaga ctactggtaa aactgaattt tgggcaactt
      661 caagtgatgt tttaaaatta aaagcaaatt acacaatcga tgattctgtt aaagagggcg
      721 atatatttac ttttaaatat ggtcaatatt tccgtccagg atcagtaaga ttaccttcac
      781 aaactcaaaa tttatataat gcccaaggta atattattgc aaaaggtatt tatgatagta
      841 caacaaacac aacaacatat acttttacga actatgtaga tcaatataca aatgttagag
      901 gtagctttga acaagttgca tttgcgaaac gtaaaaatgc aacaactgat aaaacagctt
      961 ataaaatgga agtaacttta ggtaatgata catatagcga agaaatcatt gtcgattatg
     1021 gtaataaaaa agcacaaccg cttatttcaa gtacaaacta tattaacaat gaagatttat
     1081 cgcgtaatat gactgcatat gtaaatcaac ctaaaaatac atatactaaa caaacgtttg
     1141 ttactaattt aactggatat aaatttaatc caaatgcaaa aaacttcaaa atttacgaag
     1201 tgacagatca aaatcaattt gtggatagtt tcacccctga tacttcaaaa cttaaagatg
     1261 ttactgatca attcgatgtt atttatagta atgataataa aacagctaca gtcgatttaa
     1321 tgaaaggcca aacaagcagc aataaacaat acatcattca acaagttgct tatccagata
     1381 atagttcaac agataatgga aaaattgatt atactttaga cactgacaaa actaaatata
     1441 gttggtcaaa tagttattca aatgtgaatg gctcatcaac tgctaatggc gaccaaaaga
     1501 aatataatct aggtgactat gtatgggaag atacaaataa agatggtaaa caagatgcca
     1561 atgaaaaagg gattaaaggt gtttatgtca ttcttaaaga tagtaacggt aaagaattag
     1621 atcgtacgac aacagatgaa aatggtaaat atcagttcac tggtttaagc aatggaactt
     1681 atagtgtaga gttttcaaca ccagccggtt atacaccgac aactgcaaat gtaggtacag
     1741 atgatgctgt agattctgat ggactaacta caacaggtgt cattaaagac gctgacaaca
     1801 tgacattaga tagtggattc tacaaaacac caaaatatag tttaggtgat tatgtttggt
     1861 acgacagtaa taaagatggt aaacaagatt cgactgaaaa aggaattaaa ggtgttaaag
     1921 ttactttgca aaacgaaaaa ggcgaagtaa ttggtacaac tgaaacagat gaaaatggta
     1981 aataccgctt tgataattta gatagtggta aatacaaagt tatctttgaa aaacctgctg
     2041 gcttaactca aacaggtaca aatacaactg aagatgataa agatgccgat ggtggcgaag
     2101 ttgatgtaac aattacggat catgatgatt tcacacttga taatggctac tacgaagaag
     2161 aaacatcaga tagcgactca gattctgaca gcgattcaga ctcagatagc gactcagatt
     2221 cagatagcga ctcagattca gacagcgatt cagacagcga ctcagactca gatagcgatt
     2281 cagattcaga cagcgactca gactcagaca gcgattcaga ctcggatagc gactcagact
     2341 cagatagcga ctcagattcg gatagcgact cagactcaga tagcgattca gattcagata
     2401 gcgattcgga ctcagacagt gattcagatt cagactcaga tagcgactca gattctgaca
     2461 gcgattcaga ctcagacagc gactcagact cagacagtga ttcagattca gacagcgact
     2521 cagattcaga tagcgactca gactcagata gcgactcaga ctcagatagc gactcagact
     2581 cggatagcga ttcagattca gacagcgact cagattcaga tagcgattcg gactcagaca
     2641 acgactcaga ttcagatagc gattcagatt cagatgcagg taaacatact ccggctaaac
     2701 caatgagtac ggttaaagat cagcataaaa cagctaaagc attaccagaa acaggtagtg
     2761 aaaataataa ttcaaataat ggcacattat tcggtggatt attcgcggca ttaggatcat
     2821 tattgttatt cggtcgtcgt aaaaaacaaa ataaataata tgattaactt aaccaggtcc
     2881 atgtggcctg gttttttctg tttagaaatt cccagatata aaacaaaaaa tctttaagga
     2941 atagatacaa tttaatttgt tacagaaatg taattgtcta tgaattagaa aagctaagat
     3001 attaatttca agggcaaata a
//
LOCUS       DAKTGS010000194         2985 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.201, whole genome shotgun sequence.
ACCESSION   DAKTGS010000194 DAKTGS010000000
VERSION     DAKTGS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.201"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(3..242)
                     /locus_tag="O0286_002692"
     CDS             complement(3..242)
                     /locus_tag="O0286_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171018.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            475..2139
                     /gene="alsS"
                     /locus_tag="O0286_002693"
     CDS             475..2139
                     /gene="alsS"
                     /locus_tag="O0286_002693"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCY0171019.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            2176..2880
                     /gene="budA"
                     /locus_tag="O0286_002694"
     CDS             2176..2880
                     /gene="budA"
                     /locus_tag="O0286_002694"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0171020.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
BASE COUNT         1104 a          451 c          544 g          886 t
ORIGIN      
        1 aactataaaa ccaatcgata aatataagta tgaaatgtat aagtcacagt tagatctaag
       61 tcttgctgtg gctttttata tctccataaa acgacaaatt caagcccgac ataaaacagc
      121 attttcagcc caccataaaa cgacaatttc agcccgccat tgactaaaca ccacatccca
      181 aaaatatcgt aacaatcctc tacatcaatc aatccaacat ccctcataat cacaacgcac
      241 aaaatctatt catgcatttt tggaatactt agtattacaa ataacgattt ttattcatct
      301 aacaaaggtt atataatgta ctgaaggcaa tttttatgta ttacaaatct aatcgtacat
      361 gtaaaatttt gataaacatc attaattttg cgtaactatc attagattac aaatcacaaa
      421 gtaattacat gtaatacaca tctatacatc acatttgaag ggaaatgaat ataaatgact
      481 gataaaaagt acactgcagc cgatatggtt attgatactt tgaaaaataa tggggtagaa
      541 tatgtttttg gtattccggg tgcaaagatt gactatctat ttaatgcttt aattgatgat
      601 ggtcctgaac ttattgtcac tcgtcatgaa caaaacgccg caatgatggc gcaaggtatt
      661 ggaagattaa caggcaaacc gggtgtagta cttgttacaa gtggtcctgg tgtaagtaat
      721 ttaactactg gattattaac cgcaacatct gaaggggatc ctgtattagc gttaggtggc
      781 caagtgaaac gtaatgattt attacgatta acgcatcaaa gtattgataa tgctgcgcta
      841 ttaaaatatt catcaaaata tagtgaagaa gtacaagatc ctgaatcatt atcagaagtt
      901 atgacaaatg caattcgaat tgctacttca ggaaaaaatg gcgcaagttt tattagtatt
      961 ccgcaagacg ttatttcttc accagttgaa tctaaagcta tatcactttg ccaaaaacca
     1021 aatttaggag taccgagtga acaagatatt aatgatgtca ttgaagcgat taaaaatgca
     1081 tcatttcctg ttttattagc tggtatgaga agttcaagtg cagaagaaac aaatgccatt
     1141 cgcaaattag ttgagcgcac gaatttacca gttgtagaaa cattccaagg tgcaggtgta
     1201 attagtcgtg aattagaaaa tcatttcttc ggtcgtgtgg gcttattccg caatcaagtt
     1261 ggtgatgaat tattacgtaa aagtgattta gttgttacaa tcggttatga tccaattgaa
     1321 tacgaagcta gtaactggaa taaagaatta gaaacacaaa ttatcaatat tgacgaagtt
     1381 caagctgaaa ttactaatta tatgcaaccg aaaaaagagt tgattggtaa tattgctaaa
     1441 acgattgaaa tgatttctga aaaagtggat gagccattta taaatcaaca acatttagac
     1501 gaattagaac aattaagaac acatattgat gaagaaactg gtattaaagc gacgcatgaa
     1561 gaaggaattc tacatccagt ggaaattatt gaatctatgc aaaaggtatt aactgatgat
     1621 actactgtaa cagttgatgt tggaagtcac tatatttgga tggcacgtaa tttcagaagt
     1681 tacaatccaa gacatttatt atttagcaat ggtatgcaaa cgcttggtgt agcattaccg
     1741 tgggcaattt cagctgcact tgtgcgccct aatacgcaag ttgtgtccgt tgctggcgat
     1801 ggtggctttt tattttcatc acaagattta gaaacggccg tacgtaaaaa tttaaatatc
     1861 atccagctta tttggaatga tggaaaatat aacatggttg aattccaaga agaaatgaaa
     1921 tataaacgtt cgtcaggtgt agacttcggt cctgtagatt ttgtaaaata tgcagaatca
     1981 tttggcgcga aaggtttacg agttactaat caagaagaat tagaagcggc aattaaagag
     2041 ggctatgaaa cagatggtcc agtattaatt gatatacctg taaattacaa agataatatc
     2101 aaactttcaa caaatatgtt acctgacgta tttaactaaa ataaagataa atgttaaaga
     2161 ggagtgggag attttatgac taatgtttta taccaacatg gtacattagg cacattaatg
     2221 gcaggattat taaaaggaac tgcatcaata aatgaattat tgcaacatgg tgacttaggt
     2281 atcgctacac taacaggttc aaacggtgag gtaatctttt tagatggaaa agcttaccat
     2341 gcaaatgaac ataaagaatt tgtagaatta aaaggtgacg agttaacacc atatgcaact
     2401 gtaactaaat ttgtagcaga tacaagctat gaaacgaaag ataaatcttc agaagcagtt
     2461 tttgcagaaa ttaaggaaaa gatgttgagt gaaaatttat tttcagcagt aaaaatttca
     2521 ggcttattta aaaaaatgca tgtacgtatg atgccggctc aagaaccacc ttatacacgt
     2581 ttaatcgatt cagctagaag acaacctgaa caaactgaaa cgtatgtcaa aggttcagtc
     2641 gttggtttct ttacaccaga attattccat ggtatcggat cagcaggatt tcatgtacac
     2701 tttgcgaatg atgatcgtaa ctttggtgga catgtcttag attttgaagt agaagatgtt
     2761 aaagtagaaa tccaaaatat agaaacattt gaacagcatt ttccaattca agataaagat
     2821 ttcactaaag caaatattga ctataaagat attgcagacg aaattagaga agctgaataa
     2881 tgaatccaga aatataatga cggtttatga aaattgactt cataatgcgc gatttagaaa
     2941 tgatagtttg taaatatgat taaccatgac tacaatagaa caaat
//
LOCUS       DAKTGS010000195         2956 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.202, whole genome shotgun sequence.
ACCESSION   DAKTGS010000195 DAKTGS010000000
VERSION     DAKTGS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.202"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(260..2869)
                     /gene="adhE"
                     /locus_tag="O0286_002695"
                     /gene_synonym="adhC"
     CDS             complement(260..2869)
                     /gene="adhE"
                     /locus_tag="O0286_002695"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCY0171021.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          838 a          564 c          465 g         1089 t
ORIGIN      
        1 cttttaaacc gcattatttt cgccttttta tttatatgaa attaaaaata attatactct
       61 aaagtagtaa tagtttgtga aatgagtatc aatcttaata ttttctttat ttaaaaaaat
      121 gattgtctag tttgtatctc tctgaagatt tggcaataaa taaaagccga taaccgtata
      181 atgattatcg acttaaagtt tatgtggcat tttttacttt tgtaatttca ggtgagttag
      241 atgattatta tcagatagat tattgcttat aatcatatga tgtttgaatg atatctttga
      301 tttcactgat tagtgcttct ttaggattag cagttgtaca ttgatcttca aatgcgagct
      361 ctgccattct atcaattgac tcatttaatt cttcttcaga cacaccttgt gatttcaaat
      421 tcatttcaat tccgactgat tgacctaatt cgtagacagc tttagctaat gattctacga
      481 gtgcttctgt cgtattacct tttaatccta agaatttggc aatatctgca taatctgtat
      541 ctgctctgaa gaactcatat ttagggaata atgcatgttt ttgcgggtct ttggcattat
      601 aacggataat atgcggtagt aatatcgcat tcgctctacc atgcggaata ccatattcgc
      661 caccaatttt atgcgcaatt gagtgtgcaa tgcctaagaa tgcatttgca aatgccatac
      721 cagccaaagt tgatgcgtta tgcattttct ctcttgaaac tttatcaccc ttttcaacag
      781 atgattttaa atattcgaac gtcaatttaa tcgcttgtag actcaaacct cttgtgtagt
      841 ctgaagccat tacagataca tatgattcca ttgcatgcgt tagtacatcc attcctgtat
      901 ctgctgtaac gctttttggc acactcatca caaattgagg gtcaataatt gcaacgtcag
      961 gtgttaaagc aaaatcagcc aacggatatt ttacatttgt ttcactatct gtgataactg
     1021 caaatggtgt tacttctgaa cctgtacctg atgtcgtagg gatacaaatg aacgtcgcat
     1081 tttcaggcat gcctatttta taagtacgtt taccgatgtc taggaacttt tgtttagcac
     1141 cgaagaatga tgtctcaggg tgttcaaaga acatccacat tgcttttgca gcatccatcg
     1201 ctgaaccacc accaagtgca atgattgtat ccggttggaa atcaaccatc atttccagac
     1261 ctttatatac tgtattagtt gatgggttcg gttcgacttc gctaaatatt ttaatttgag
     1321 gctgttccgt tttttgtctt aatacattct caactgtttt tgtataaccg aattctacca
     1381 taccagggtc acagacaatc atcacttttt caatcttgtc cattgttgtt agactcatga
     1441 ttgcattttc ttcaaaataa atttgagcag gcaccttgaa aatttgagta ttattacgtc
     1501 gtttagcaat cgttttaatg tttaataaat ctgtcgcact aacattatgt gaaattgagt
     1561 ttctaccgta ggaaccacaa cctaatgtta aagacggaat caattcgtta tacatatcac
     1621 caatacctcc aaccgctgat ggtgtattta caagtacacg acaagctttc attcttagtc
     1681 caaaatcttt ttgtaatgtt tcatcttctg tatggataac ggctgtgtgt cctaatccac
     1741 caaaatgtag tgtgtcttca caaatttgaa atgcttgttt tgtagattgg gcttttacta
     1801 aggctaatac tggagataat ttttcacgag ataacggata gtctgaacct acaccgctaa
     1861 tttcggctat gataagtttt gtattttcgg ggacaggtat acctgctaat tcagctattt
     1921 caactgcaga tttaccgaca atatcaggct taatacctgt tttttgttca ttcataattg
     1981 cattttctaa gcgttgtaat tcatcttttt taacaaagta tgcttgatgt gctttaaatt
     2041 cattagtaac atctttataa atttctttat caatgactac aacttgttca gaagcacaaa
     2101 tcataccatt atcaaatgtt tttgaaccaa tgatatcatt tactgcacgt ttaatgtgtg
     2161 ctgttttttc aatgtaagac ggcacgttac ctggtcccac acctaatgcc ggtttgccag
     2221 ttgaatatgc agacttaacc atgcccgaac cacctgttgc tagaactaat gcaatacctt
     2281 tgtgattcat taattgtttt gttgcttcga tagaaggcac ttcaatccac tgaataatat
     2341 ctttaggtgc acctgccttc attgccgctt ctaatacaac ttctgctgca cgcttcgacg
     2401 attcttgtgc acttggatgg aatgcaaaaa tgattggatt tcctgtctta attgcaatca
     2461 tcgctttaaa aatagttgtc gacgtaggat ttgttgttgg cgtaacacca caaataacac
     2521 caattggttc cgctacatac gttaatcctt tttctttatc ttcaccaata atccctactg
     2581 tcttattgtc ttttattgaa ttccatatat attcagaagc gtataaattt ttaatcgctt
     2641 tatcttcgta tatacctctt ccagtttctt catgtgctaa ttttgctagc accatatgtt
     2701 gatcaacagc tgctaagctc atttgatgaa caatatgatc aatttcttct tgtgactttt
     2761 tagataatgc ttctaatgct tttttccctt tgtcagctag agcatcaatc ataattgcca
     2821 cttcttgttc tttcgatcca cgattttctt tttcaggtat agttaacata tacaaccact
     2881 cctttatact ttgtgaatta tttcacaaac attatagtac atgtctctca ggatataaag
     2941 aaaattctat acaaaa
//
LOCUS       DAKTGS010000196         2911 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.203, whole genome shotgun sequence.
ACCESSION   DAKTGS010000196 DAKTGS010000000
VERSION     DAKTGS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.203"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            17..418
                     /locus_tag="O0286_002696"
     CDS             17..418
                     /locus_tag="O0286_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCY0171022.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            418..675
                     /locus_tag="O0286_002697"
     CDS             418..675
                     /locus_tag="O0286_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCY0171023.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            678..878
                     /locus_tag="O0286_002698"
     CDS             678..878
                     /locus_tag="O0286_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171024.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            893..1135
                     /locus_tag="O0286_002699"
     CDS             893..1135
                     /locus_tag="O0286_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171025.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            1150..1398
                     /locus_tag="O0286_002700"
     CDS             1150..1398
                     /locus_tag="O0286_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0171026.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            1391..1927
                     /locus_tag="O0286_002701"
     CDS             1391..1927
                     /locus_tag="O0286_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCY0171027.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            1964..2170
                     /locus_tag="O0286_002702"
     CDS             1964..2170
                     /locus_tag="O0286_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0171028.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            2167..2370
                     /locus_tag="O0286_002703"
     CDS             2167..2370
                     /locus_tag="O0286_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171029.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            2363..2599
                     /locus_tag="O0286_002704"
     CDS             2363..2599
                     /locus_tag="O0286_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171030.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            2592..>2911
                     /locus_tag="O0286_002705"
     CDS             2592..>2911
                     /locus_tag="O0286_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171031.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIG"
BASE COUNT         1162 a          396 c          620 g          733 t
ORIGIN      
        1 atttagaggt ggaatgatgg ctagaagaaa agttataaga gtgcgtatca aaggaaaact
       61 aatgacattg agagaagttt cagaaaaata tcatatatct ccagaacttc ttagatacag
      121 atacaaacat aaaatgcgcg gcgatgaatt attgtgtgga agaaaagact caaaatctaa
      181 agatgaagtt gaatatatga agagtcaaat aaaagatgaa gaaaaagaga gagaaaaaat
      241 cagaaaaaaa gcgattttga acctatacca acgaaatgtg agagcggaat atgaagaaga
      301 aagaaagaga agattgagac catggcttta tgatggaacg cctcaaaaac attcacgtga
      361 tccgtactgg ttcgatgtca cttataacca aatgttcaag aaatggagtg aagcataatg
      421 agcataatca gtaacagaaa agtagatatg aatgaaacac aagacaatgt taaacaacct
      481 gcgcattaca catacggcga cattgaaatt atagatttca tcgaacaagt tacggcacag
      541 tatccaccac aattagcatt cgcaataggt aatgcaatca aatacttgtc tagagcaccg
      601 ttaaagaatg gtcatgagga tttagcaaag gcgaagtttt acgtcgatag agtgtttgac
      661 ttgtgggagg ggtaacgatg gcaacgcaaa aacaagttga ttacgtaatg tcattacagg
      721 agcaactgga attagaagac tgcgaaaaat atacagacga acaagttaaa gcaatgagtc
      781 ataaagaagt tagcaatgtg attgagaact ataagacaag cataaggaat gaagaactat
      841 attacgaatg catgtcgttt ggactgccta attgttaaaa ggagtgacga ccatgacaga
      901 tagcgcacgt aaagaacgct taaaccaatt tttcggctct aaaagatatc tgtatcagga
      961 taacgaacga gtggcacata ctcatgtagt aaatggcacg tattactttc atgggcatat
     1021 cgtgccaggt tggcaaagcg ttaaaaagac atttgatact gctgaagagc tcgaaatata
     1081 tataaagcaa catggtttgg aatacgagga acagaagcaa ctaactttat tttagaggag
     1141 atggaaatga tgaataaccg cgaacaaatt gaacaatcaa ttatcagtgc tagtgcgtat
     1201 aacggcaatg acacagaggg attactaaaa gagattgaag acgtgtataa gaaagcgcaa
     1261 gcgtttgatg aaatacttga aggtttacct aatgctatgc aagatgcact caaagaagat
     1321 attggtcttg atgaagcagt agggattatg acggggcaag tggtctataa atatgaggag
     1381 gatcaggaaa atgactaata cattaacaat tgatcagtta caagagttat tacaaataca
     1441 aaaggagttc gacgatagaa taccaacgct gaacttacga gatagcaaaa tagcatatgt
     1501 agttgaattc tttgaatggt ttaatacatt ggaaacgttt aagaactgga agaagaaacc
     1561 aggtaagccg ttagatgttc agttagatga attagcggac atgttggcgt ttggattgag
     1621 tattgcgaat caagtaggag tgtcatcaga agagataaaa gaagcgattg aatcaagttt
     1681 taaaaatagt gaatttcaca acatgtttaa ttttaaagat aaagaatttg ctcaagacgc
     1741 agttgttagc acaccacaga taatattcaa agaattttat cctgaccaat tggcaattgt
     1801 aatagcgata gacatagctt tcaacttata ttctatcgac cagctcattg acgcatacaa
     1861 aaagaaaatg aaaaggaatc atgaaagaca agatggaaca gcagacgcag gaaaaggata
     1921 cgtgtaaaga catcttagat cgagtcaagg aggttttggg gaagtgacgc aatacttagt
     1981 cacaacattc aaagattcaa caggacgcaa gcatacacac ataactaaag ctaaaagcaa
     2041 tcaaaggttt acagttgttg aggcagagag taaagaagaa gctgagcgca aatacgaggc
     2101 acaagttaaa agagatgcag ttattaaagt gggtcagtta tttgaaaata taagggagtg
     2161 tgggaaatga cggaggttaa aattaaaact atttcagata gagtttatta cacaacaaca
     2221 gatctagctt ctgacgatta tattaatctt gttatgaatc tagtgattga ggattttctt
     2281 ccggtcaaag atgtgttcaa caatgaagta tgggttaaaa gagatgagat tgaatcattt
     2341 acatttatta aggaggcaaa cgatgattaa catacctaaa atgaaattcc cgaaaaagta
     2401 cactgaaata atcaaaaaat ataaaaataa agcacctgaa gaaaaggcta agattgaaga
     2461 tgattttatt aaagaaatta aagataaaga cagtgaattt tacagtccta cgatggctaa
     2521 tatgaatgaa tatgaattaa gggctatgtt aagaatgatg cctagtttaa ttgatactgg
     2581 agatgacaat gatgattaaa aaacttaaaa atatggatgg gttcgacatc tttattgttg
     2641 gaatactgtc attattcggt atattcgcat tgctacttgt tatcacattg cctatctata
     2701 cagtggctag ttaccaacac aaagaattac atcaaggaac tattacagat aaatataaca
     2761 agagacaaga taaagaagac aagttctata ttgtattaga caacaaacaa gtcattgaaa
     2821 attccgactt attattcaaa aagaaatttg atagcgcaga tatacaagct aggttaaaag
     2881 taggcgataa ggtagaagtt aaaacaatcg g
//
LOCUS       DAKTGS010000197         2911 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.204, whole genome shotgun sequence.
ACCESSION   DAKTGS010000197 DAKTGS010000000
VERSION     DAKTGS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.204"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(428..1141)
                     /locus_tag="O0286_002706"
     CDS             complement(428..1141)
                     /locus_tag="O0286_002706"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCY0171032.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            1401..1703
                     /locus_tag="O0286_002707"
     CDS             1401..1703
                     /locus_tag="O0286_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171033.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            1959..2324
                     /gene="crcB"
                     /locus_tag="O0286_002708"
     CDS             1959..2324
                     /gene="crcB"
                     /locus_tag="O0286_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCY0171034.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            2321..2674
                     /locus_tag="O0286_002709"
     CDS             2321..2674
                     /locus_tag="O0286_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCY0171035.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT          920 a          482 c          383 g         1126 t
ORIGIN      
        1 ctttcatatt attttcaaat tatctatctg atatttattt tattgaactt gagtcaccac
       61 ttaaactcaa aaatacgtgt catcaagtgc aaaataattt agaactaata caaacataaa
      121 aacttttact ttacgcttaa aatcaaaagt cacataccta gatataagta caacttgtat
      181 attttgtact ctaataggta tgtggcttaa ataatattta attcttgagt tgcatatagt
      241 aactgttttt attcattttc taaaatatgc aagactaatt tccttttcta ctcctgttga
      301 atcaatttag acttttttat aactcgagct tttacttagc tgaattttga cttcttcttc
      361 attaataact atgatggaaa aacccccata agcacaatgc ctatgagggt ttaaatatat
      421 ttatatatta tagaatagaa agacctgaag attgaatatc tttcgcaaag cctttaactg
      481 tatctactga taattcgtta atatcgcgac ctaagtttgt attcactttt ttcacaacat
      541 ctgctgggca tgtaataata tctgcaccaa tttcatcagc ttgaatcaca ttgaataatt
      601 cgcggcaact tgcccataat aatttaacgc cgtctttact atgcgtaact tttacagcct
      661 ctttcattaa tggtaatgga tctacgcctg tatctgcaat acgtcctgca aatactgaaa
      721 catatgttgg cacaccttca gttactgctt cagttatttc tttaacttgt tcaattgtgt
      781 aaacagccgt aacgtttaat ctcacattgt cagctgaaag ttttttaatt aaaggaatcg
      841 ttgattcacc ttttgtattt acaataggaa ttttaacaaa tacattttcg ccatattgtt
      901 ttaaaattgc tgcttctttt tccatagttt ctaaatcgtc tgcaaatact tcaaatgaaa
      961 ttgaagcatc tggaatttct ttcacagctt cttcagcaaa agctttgtaa tctgttacgc
     1021 ccgctttagc cattaaacta ggatttgttg taaaaccatc cacttgtttg tttttataag
     1081 ctgctttcat ttcttcaata tctgcaccgt ccgcaaatac ttctatattt agtttagcca
     1141 tataatatag cctccttgat tcttattaaa attttaacaa catctgcatg tctttttctt
     1201 acaaccattt gtaaaaaatg atttttattt ctttgttata taaaacattt atatcacgct
     1261 tttataatat atagctaatt ttttgcctat attttttaaa aatgacttta gcaaacattg
     1321 tgtctacaag ttaataaaac tattgtttta tgattgttta tgatacgata tgtaaatgat
     1381 aatcatccag gaggtctagc atgtctcgtt caaaaaaata cttttactta tctagcttaa
     1441 tgattatttt aagctttttc tttaatacaa ataacgtttt cctaagtgga ctttttaatt
     1501 cttttattaa attaatactt ttctgcagtg ttattaactc aattgtacta attttgtcta
     1561 taatttttgc agatcgttca attaaatcac taaagcctga tgcagattgg attagaattg
     1621 cgagtaaaag tttgccttgg attattctaa ttgttatttt agtacatatc ttttcaattg
     1681 ttcgtacatt cggttttatt taaaaaagtt aatatgtcat tgtagcctta atacaaaaca
     1741 atacaatgta tcatgctata atgagtaaaa caatttgata acgttgttgc gtataaaaaa
     1801 tattagattt tcgaaatcat aactatgcat ctaatcgcta tagttataca acaagatata
     1861 acatataatg aggtttgata atgcatcgac aatttttgtc gtcgcgttgc caaaacctct
     1921 tttttaaatt caaactactt cttttcgagg tgaaccaaat gcaatatgta tatattttta
     1981 tcggtggtgc tttaggcgct ttattacgtt acctcatttc ttttctgaat actgacggag
     2041 gttttccaat cggaacactg atagccaatt tgactggtgc ctttgtaatg ggattgctaa
     2101 cagccttaac aattgcattt ttttcaaacc atccgaccct aaaaaaagct attacgactg
     2161 gttttcttgg tgctttaacg actttttcaa catttcaatt agaattaata catatgtttg
     2221 atcatcaaca atttataact ttactactat atgctgtaac aagttatgtc tttggtattt
     2281 tgttatgtta cgtcggtata aaactaggtg gtgatttatc atgatatcaa tcattttagt
     2341 catgattggc ggcggttttg gcgcaattgc tagaagtgcc attactgatt attttaatca
     2401 taaatttact tcaaagttac ctatcgcaac attgatagta aatctagttg gtagtttttt
     2461 aattggatta actataggct tatcaatttc aatctcatgg ttccctgcgt tctttgttac
     2521 cggtttttta ggtggcttaa caactttctc aacgttagcc aaagaactta cactaatgat
     2581 gacgccaaaa tttaatatta acctttttct caattattca cttttacaat tcatcattgg
     2641 atttatagct tgttatattg gctatcatat ttaaaaataa aatgcttcat tcagcaaata
     2701 ggtaaattac gacaccttcc tgaacgaagc attttttaat tttcatgcaa atttttaagc
     2761 accatataat gcctaccaaa tttcaataat ctttgttgcc gtttaaataa tgtgaatgtc
     2821 aataaattct ccaaactagt cgaaaataaa gggagtggga cataaatccc taaaaaaaag
     2881 cagtaagata attttcaatt agaaaatatc t
//
LOCUS       DAKTGS010000198         2827 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.205, whole genome shotgun sequence.
ACCESSION   DAKTGS010000198 DAKTGS010000000
VERSION     DAKTGS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.205"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            87..1349
                     /gene="femA"
                     /locus_tag="O0286_002710"
     CDS             87..1349
                     /gene="femA"
                     /locus_tag="O0286_002710"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCY0171036.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            1368..2627
                     /gene="femB"
                     /locus_tag="O0286_002711"
     CDS             1368..2627
                     /gene="femB"
                     /locus_tag="O0286_002711"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCY0171037.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
BASE COUNT         1051 a          368 c          511 g          897 t
ORIGIN      
        1 atttttgaca tttgcagagg ggaaatagaa aaactgcaaa tacggaaatg aaattaatta
       61 acgagagaca aataggagta atgataatga agtttacaaa tttaacagct aaagagtttg
      121 gtgcctttac agatagcatg ccatacagtc atttcacgca aactgttggc cactatgagt
      181 taaagcttgc tgaaggttat gaaacacatt tagtgggaat aaaaaacaat aataacgagg
      241 tcattgcagc ttgcttactt actgctgtac ctgttatgaa agtgttcaag tatttttatt
      301 caaatcgcgg tccagtgatt gattatgaaa atcaagaact cgtacacttt ttctttaatg
      361 aattatcaaa atatgttaaa aaacatcgtt gtctatacct acatatcgat ccatatttac
      421 catatcaata cttgaatcat gatggcgaga ttacaggtaa tgctggtaat gattggttct
      481 ttgataaaat gagtaactta ggatttgaac atactggatt ccataaagga tttgatcctg
      541 tgctacaaat tcgttatcac tcagtgttag atttaaaaga taaaacagca gatgacatca
      601 ttaaaaatat ggatggactt agaaaaagaa acacgaaaaa agttaaaaag aatggtgtta
      661 aagtaagatt tttatctgaa gaagaactac caatttttag atcatttatg gaagatacgt
      721 cagaatcaaa agcttttgct gatcgtgatg acaaatttta ctacaatcgc ttaaaatatt
      781 acaaagaccg tgtgttagta cctttagcgt atatcaactt tgatgaatat attaaagaac
      841 taaacgaaga gcgtgatatt ttaaataaag atttaaataa agcgttaaag gatattgaaa
      901 aacgtcctga aaataaaaaa gcacataaca agcgagataa cttacaacaa caacttgatg
      961 caaatgagca aaagattgaa gaaggtaaac gtctacaaga agaacatggt aatgaattac
     1021 ctatctctgc tggtttcttc tttatcaatc catttgaagt tgtttattat gctggtggta
     1081 catcaaatgc attccgtcat tttgccggaa gttatgcagt gcaatgggaa atgattaatt
     1141 atgcattaaa tcatggcatt gaccgttata atttctatgg tgttagtggt aaatttacag
     1201 aagatgctga agatgctggt gtagttaaat tcaaaaaagg ttacaatgct gaaattattg
     1261 aatatgttgg tgactttatt aaaccaatta ataaacctgt ttacgcagca tataccgcac
     1321 ttaaaaaagt taaagacaga attttttagg aagggaatta tcaaaacatg aaatttacag
     1381 agttaactgt taccgaattt gacaactttg tacaaaatcc atcattggaa agtcattatt
     1441 tccaagtaaa agaaaatata gttacccgtg agaatgatgg ctttgaagta gttttattag
     1501 gtattaaaga cgacaataac aaagtaattg cagcaagcct tttctctaaa attcctacta
     1561 tgggaagtta tgtttactat tcgaatcgtg gtccagtaat ggatttttca gatttaggat
     1621 tagttgatta ttatttaaaa gagttagata aatatttaca gcaacatcaa tgtttatatg
     1681 ttaaattaga tccgtattgg ttatatcatc tatatgataa agatatcgtg ccatttgaag
     1741 gtcgcgagaa aaatgatgcc ctagtaaact tgtttaaatc acatggttac gagcatcatg
     1801 gctttacaac tgagtatgat acatcgagcc aagtacgatg gatgggcgta ttaaaccttg
     1861 aaggtaaaac acccgaaaca ttgaaaaaga catttgatag tcaacgtaaa cgtaatatta
     1921 ataaagcgat aaactatggt gttaaagtca gattccttga acgtgatgag ttcaatcttt
     1981 tcttagattt atatcgtgaa actgaagagc gtgctggatt tgtatcaaaa acagatgatt
     2041 atttttataa ctttattgac acatatggag ataaagtatt agtaccatta gcatatattg
     2101 accttgatga atatgtgtta aagttgcaac aggaattgaa tgacaaagaa aatcgtcgtg
     2161 atcaaatgat ggcgaaagaa aacaaatcag ataagcaaat gaagaaaatt gcagaattag
     2221 ataagcaaat tgatcatgat cagcatgaat tattgaatgc aagtgaattg agcaaaacgg
     2281 acggcccaat tctaaacctt gcttctggcg tttattttgc aaatgcatat gaagtgaatt
     2341 atttctctgg tggttcatca gaaaaatata atcaatttat gggaccatac atgatgcatt
     2401 ggtttatgat taactattgc ttcgataatg gctatgatcg ttataatttc tatggtttat
     2461 caggtgattt tacggaaaac agtgaagatt atggcgtata ccgctttaaa cgtggattta
     2521 atgtacaaat cgaagaatta ataggggatt tctataaacc aattcataaa gtgaaatatt
     2581 ggttgttcac aacattggat aaattacgta aaaaattaaa gaaatagatg taaatgatgt
     2641 tagggcttat agttattgat actataggct cttttttata tgttttttaa taaagcctcg
     2701 aataatgata tattaaatga caagtttaac aagtgattgc aataaatata aatatgaatt
     2761 gcacaaccta tttgtaaatg attgagttga gtatactttt taattcattc aatatgactc
     2821 aaaacaa
//
LOCUS       DAKTGS010000199         2748 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.206, whole genome shotgun sequence.
ACCESSION   DAKTGS010000199 DAKTGS010000000
VERSION     DAKTGS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.206"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..154
                     /locus_tag="O0286_002712"
     CDS             <1..154
                     /locus_tag="O0286_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0171038.1"
                     /translation="KKLSFTAGIRSVDDFQFDTDISYTDELGRKFNKNPKSVSEIKKE
                     QNTSNK"
     gene            348..524
                     /locus_tag="O0286_002713"
                     /pseudo
     CDS             348..524
                     /locus_tag="O0286_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(502..603)
                     /locus_tag="O0286_002714"
     CDS             complement(502..603)
                     /locus_tag="O0286_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171039.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(726..821)
                     /gene="pepA1"
                     /locus_tag="O0286_002715"
     CDS             complement(726..821)
                     /gene="pepA1"
                     /locus_tag="O0286_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCY0171040.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            1024..1167
                     /locus_tag="O0286_002716"
     CDS             1024..1167
                     /locus_tag="O0286_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0171041.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1771..2154
                     /locus_tag="O0286_002717"
     CDS             1771..2154
                     /locus_tag="O0286_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171042.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            2165..2341
                     /locus_tag="O0286_002718"
     CDS             2165..2341
                     /locus_tag="O0286_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171043.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            2343..2528
                     /locus_tag="O0286_002719"
     CDS             2343..2528
                     /locus_tag="O0286_002719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171044.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
BASE COUNT         1041 a          345 c          469 g          893 t
ORIGIN      
        1 taaaaagtta tcatttacag ctggtataag atctgttgat gattttcaat ttgataccga
       61 tatttcttat acagatgaat tgggtagaaa atttaataaa aatcctaagt cagtttctga
      121 aataaaaaaa gagcaaaata cgtccaataa ataattgttc atattgtgat gaaacaaaaa
      181 tataagtcat tagatgagtt taactatgtt ataaatattt gtagtatcta taaaaatctc
      241 gacactatta aaatgataaa gtgccgaggt tttcttactt atttagttaa ttcaaagttt
      301 atgccagatt cataagaatt tgtgacactt ttaatagtgt aaccattgat tattacaatt
      361 tatcaaatgg tcctttagaa ggtataaata acaaaataaa attaattaaa cgtacatctt
      421 ttggttatgg aagttacaat catttgcgca atcgaatatt attatgttca aaactttacg
      481 ctccaaaaag taaaaaggaa gttaagcaat gtttagttgc ttaacttcgg atattgaacg
      541 catcagtcca atttgacata gagccttttt tagttcttga tgtttctctt taaaaccttg
      601 catattttac aaaaagaaaa attagcagta taattaagac aacgaaaata agtatttact
      661 tatacaccaa tcccctcact atttgcggta gtgaggggat ttttattggt gcggctatat
      721 gtcacctatt ttgtattgcg tctacttagc caataagaaa aaaacgcaat ggcacagcca
      781 ctgatgactg gtgctatgat gtgaacgaaa ataagcatca ccttatacac ctcctctctg
      841 cgtctaaatt gacgcctgag agataggcga ctctactatt atatcatcgg caaatataca
      901 agcacagtca cttgcttctg ataagttata tgattctagc tgatagattg aatcgtctac
      961 acttaattgg acaaattcta tgagaataga tattgttaat ttaagaaagt aggcgatttt
     1021 attatgacaa gagaaagaag atcatttagt tcagagttta agttacaaat ggttagatta
     1081 tataaaaatg gtaagcctag gaatgaaatt atacgcgagt atgatttcac accttcgacg
     1141 tttgtaaatg gcggttataa aatgtaggaa aatggataaa gcaacatcaa aacacgggta
     1201 cattcaatca ccaagataac ttatcggatg aagaaaaaga gctgattaaa ttacgcaaag
     1261 aagttcaaca tttaaaaatg gagaacgata ttttaaagca agtagcgctg attatggggc
     1321 aaaaatagaa gtcattcaaa agaatgcaca tcaatattca gtatcagcaa tgtgtaaagt
     1381 cctgataata ctaagaagta cctattatga ttctataaaa agaaaagata ataaaatcac
     1441 taaagatgat tcaaacatag aacatgccgt cataaatatt tttaattcta atagaaaagt
     1501 ctttggtaca agacgaatta aaaatcattt aaatgacaag ggtctcactg tatctggaca
     1561 aaagataggt cgatcatgaa aaaatctagt ttctgtttat acgaaatcta aatacaaaaa
     1621 tcatctaaaa gaaactaatg aaaaacgaat taaaaatctt tattattagc tgctggtgta
     1681 ttatttgtta gtccaatttc attatcttat aattcagatg tagctcatgc tgaagataag
     1741 ttagaccatt ctcaagcaaa ggtaatatat ttgagtaacc aaaatttatt tgatgaactt
     1801 gagaaaaaag gttataaact ggaagatata tttacaaaag aagaaataaa aaaatataaa
     1861 gctgaagacc aattgagagc gggtaaaact caatatgtag aaacaggtaa agatactgca
     1921 acattatatc tttcttctgc atatacaaaa acaatagctg ctttaggtgc gggggctatt
     1981 agcgttattt ctgcactaac aggaggttta gttggcgcag gtgttggtgg tttccttgga
     2041 tctatagctg catcgaatat tgatactagt aagggaatat atataaaatt aaaaactaaa
     2101 aagaatgcag ccggggaata cgttctgaca ggagaaaaat ggggatatca gtaagggtga
     2161 ttttatggat agattaaaat attcacttaa agttggaatt ttagcattat tattattttg
     2221 tactttaaat tatttagttc caatgcaaag caatgctttt tcaataatta tatattcggc
     2281 aatttttgct gtgttactta tgcttttagt ttatatattt ttaggaattt taaagaaatg
     2341 acatgaaaca aattagcatt ggctatgaag aaatctatgg ggatagaatt tttcatagcc
     2401 attttttaaa agagcatgaa gtaaaaaatt ggaaaaccgt caatcaaata attgaaaaag
     2461 aacacttgga caaaaatgaa tatgaagcgg aaccgccgtt atttttcttt tcaggatcgt
     2521 tagattagat tcggttgtat agtataatga aaaatattat aataaattta taaaaaaggt
     2581 attttatttt gaaatcatgt cccttcgtta catatagtag tagaggggtt ttattttaat
     2641 aatgtttaca cctatagtta taaatttatt aaatgatgta ggttcgattt ttttagttag
     2701 ggtggggaat caattgaaag ttattgagaa agttcattat gaagatat
//
LOCUS       DAKTGS010000200         2739 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.207, whole genome shotgun sequence.
ACCESSION   DAKTGS010000200 DAKTGS010000000
VERSION     DAKTGS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.207"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..969)
                     /locus_tag="O0286_002720"
     CDS             complement(<1..969)
                     /locus_tag="O0286_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0171045.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISS"
     gene            complement(962..1512)
                     /locus_tag="O0286_002721"
                     /pseudo
     CDS             complement(962..1512)
                     /locus_tag="O0286_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            1625..2485
                     /locus_tag="O0286_002722"
     CDS             1625..2485
                     /locus_tag="O0286_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0171046.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
BASE COUNT         1031 a          383 c          397 g          928 t
ORIGIN      
        1 tgatgaaatt gagtagccta gtataaatga tcctatgaat aagtaaaatg ctgagtttat
       61 ccaaataaat agtgctgaat ttatgactaa tatgaaatat aacaaaaata tcaatacttt
      121 agttgagatt ttcttcgaaa gaatagctga aattaaacct gcacataatc ctccaatgcc
      181 atataacata tctgaaaaac caaattgtac agacgaaagt tttaaaacat tataaacata
      241 tcctggtaat gatatgttaa agatcattgt aaacaccatt ggtatgattg aaataactcc
      301 aaaaataaat atcatcatgt tgtcttttaa aaatttccat cctaataaat attcttgcaa
      361 taagctattt gttgattctt cctctgaatg agttggttta tctacatgca atctaaataa
      421 cataaaaatg ctgattagaa acatcattat agtcatcgct ataattagag tgaatccatt
      481 tattttatat aatattcctg ataatccacc tgcaataaac atacctgttt gcaaactaat
      541 ctctaataga gaatttgcat ctgtatattg atctggtttt aatatctgtt taactaaact
      601 tctagatgtt gccatatacg tagtccaacc tatcccatta acaatcgcaa atccaatcac
      661 taaataggtc tcaaatccta tcattacgag tgctatgaca atgagtaaat ataatattac
      721 ctgaagaaga taggtgatta aaataatatt tcgtctatta tatttatcgg ctagtccacc
      781 tattatagga gaagctaaga aaccggataa tacatttaaa gctaacataa tacctaatag
      841 ttgggagtcg ttagttttgt cgattaaata ccaattagcc ccaactgtac taattccaac
      901 tccgaatgct gaaattattt ctgctaaaaa gaacaatctg aagtttttaa ttttcaacaa
      961 atcagtcata aatatcacct tttttcaatg caagatttat atttgcgagc tcttctataa
     1021 attgagagtc gtttaaaata tcgaatggtt tattgccttc gccgatcaaa aatctatagt
     1081 tagtaatatt cataaactca aatattaatt tgaattggtt gactaaaggt tgtgctttgg
     1141 tatgcggatt gtcgccacct atgattaaaa tcaaatactt tttttgtgac atgatttctt
     1201 tgaagttgtc tatttgcgta tctcgcaatg actcggtcca tctatcaatg aagagtttta
     1261 aagacgcact catggagtac caatagagtg gtgttgaaaa aataataata tctgattcta
     1321 aaactttgat taaaatttgt tcgtaatcat cattatgaaa gttggcacct ttgctatgac
     1381 gattatctgt aaccttttca atgttgcttt gatataaatt cacaaagttg acttttaaat
     1441 tcttcaatag attctctact gcgatagctg aattgccacc tttctactac ttccaaataa
     1501 acaagtaatc atagtcataa ctcctttcga tttaccttta taataatatt taatatatta
     1561 ttattaaatc agaattctta gaattcagga ttcgataaaa gggaacccta aaggaggtac
     1621 tactttgaac ttaaacattt taaaatcatt tttagtaact agtgaaacaa agaaccttac
     1681 taaagcatca gagttactta actattcaca gtcaactgta tctacacata ttgaaaaatt
     1741 agaaaggcaa ttagatgtta aattatttta tagaaaaaaa tatggtatgg aactaacgga
     1801 agaaggctta gcatacgtta aatacgctaa agtgatttta gatagtaata gcgaatacga
     1861 gagagaaata aaaggacttt acaataagaa ggtaaatata agtattaaca tgcaagaaag
     1921 tcagtatttg tatcgctact ataataagat tagtgaatgg ttagctgaac acccatatgt
     1981 aaacttgaag tttaaatccg cacattctaa tttctatatt aaagaagaaa ttgctaattt
     2041 caaatcggat attagcctta tcacagacga aaagattatt aatagtaact taactgctat
     2101 tcctataact gaagaacgtt taatatttgt tactaacaaa gagatgaaag atttcaaatt
     2161 aaacgatatt caaaaccata cattacttgt tactgaaaaa gggtgtagtt acagagaaca
     2221 gttagaaatg attttatata aatatagctt ttcaacaaaa caaatgattg aatttctagg
     2281 aattgagtca ttaaaaaaac acttgaaaaa ttcaggtggg attgcgctat tgccggaatt
     2341 tattgttgca gatgaattag aaaataaaag tttgtttcaa atagacgtag atgtcgatat
     2401 tccgaattta gaaacaacat tgataattaa tcctgaatcg aataagcaag tacttgaatc
     2461 ttttgtaaaa gatgtttttt tataattatt ggtgaaaacg tgtagttatg gtgaaactca
     2521 aagataataa tttaaatgag atgttaatga aaaagtaatt caatataaaa acaggtgatt
     2581 tatatcttta ataaggatta tttctagttg aaatttcaaa ttgcgggcat tcattttcaa
     2641 aatagtaaag aatttaatag atgaaaacct ttgaatcata ctagttagta tggattgatt
     2701 aatattttaa cctgctaaat gagtagctta tgtatttta
//
LOCUS       DAKTGS010000201         2682 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.208, whole genome shotgun sequence.
ACCESSION   DAKTGS010000201 DAKTGS010000000
VERSION     DAKTGS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.208"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            71..1273
                     /locus_tag="O0286_002723"
     CDS             71..1273
                     /locus_tag="O0286_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCY0171047.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIK"
     gene            1408..1521
                     /locus_tag="O0286_002724"
     CDS             1408..1521
                     /locus_tag="O0286_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171048.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKETLFYKVSNTLCYVNNS"
     gene            complement(1525..1608)
                     /locus_tag="O0286_002725"
     tRNA            complement(1525..1608)
                     /locus_tag="O0286_002725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1572..1574),aa:Leu,seq:caa)
     gene            complement(1647..1721)
                     /locus_tag="O0286_002726"
     tRNA            complement(1647..1721)
                     /locus_tag="O0286_002726"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1686..1688),aa:Gly,seq:tcc)
     gene            complement(1825..1899)
                     /locus_tag="O0286_002727"
     tRNA            complement(1825..1899)
                     /locus_tag="O0286_002727"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1864..1866),aa:Gly,seq:tcc)
     gene            complement(1907..1980)
                     /locus_tag="O0286_002728"
     tRNA            complement(1907..1980)
                     /locus_tag="O0286_002728"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1946..1948),aa:Cys,seq:gca)
     gene            complement(1986..2057)
                     /locus_tag="O0286_002729"
     tRNA            complement(1986..2057)
                     /locus_tag="O0286_002729"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2023..2025),aa:Gln,seq:ttg)
     gene            complement(2069..2141)
                     /locus_tag="O0286_002730"
     tRNA            complement(2069..2141)
                     /locus_tag="O0286_002730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2106..2108),aa:His,seq:gtg)
     gene            complement(2144..2217)
                     /locus_tag="O0286_002731"
     tRNA            complement(2144..2217)
                     /locus_tag="O0286_002731"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2183..2185),aa:Trp,seq:cca)
     gene            complement(2233..2313)
                     /locus_tag="O0286_002732"
     tRNA            complement(2233..2313)
                     /locus_tag="O0286_002732"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2277..2279),aa:Tyr,seq:gta)
     gene            complement(2319..2394)
                     /locus_tag="O0286_002733"
     tRNA            complement(2319..2394)
                     /locus_tag="O0286_002733"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2359..2361),aa:Thr,seq:tgt)
     gene            complement(2402..2474)
                     /locus_tag="O0286_002734"
     tRNA            complement(2402..2474)
                     /locus_tag="O0286_002734"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2439..2441),aa:Phe,seq:gaa)
     gene            complement(2493..2568)
                     /locus_tag="O0286_002735"
     tRNA            complement(2493..2568)
                     /locus_tag="O0286_002735"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2533..2535),aa:Asp,seq:gtc)
     gene            complement(2578..2651)
                     /locus_tag="O0286_002736"
     tRNA            complement(2578..2651)
                     /locus_tag="O0286_002736"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2615..2617),aa:Met,seq:cat)
BASE COUNT          918 a          513 c          529 g          722 t
ORIGIN      
        1 tacacaaaaa gcgcaattgc ctctataatt taaagtgacc aaacccaaac taaaggagac
       61 aagtgcgcct atgtgtaatg ataccttaga attactaaga ataaaagatg aaaatataaa
      121 atatataaac caagaaattg acgtcattat caaaggaaaa aaagcaacag tggttaatgc
      181 tgtactaacg tataagcctt cggcctgtta ttgttgtgga gttaaaaatg aaggacaaat
      241 tcataaacat ggtaagcgtg tttctcgtat tactttactt aaaactcaag ggtataacac
      301 atacctcaac ttagctaaac aacgttttaa atgcttagaa tgcaatggca cttttactgc
      361 taaaacgtca attgttgatg agtcgtgttt tatctcaaga tgtgttactc aaaaagttat
      421 agaagaagct actaaagtta aaacagagat tgatactgca gaagataact gtatctctcc
      481 atctactgta agtcgtatta gaactaaagc ggctaattca ttacgaatta aaccctttaa
      541 ttgtttgcca gaacacatcg ctatggatga atttaaaagc gttaaaaatg taactggatc
      601 aatgagtttc atttttatag ataatgatac tcatgatgtt atagatattt tagaaaatag
      661 aactacaaga ttcttgcgtg cctatttcga gcgattcgat ttaaaaaatc gacaacaagt
      721 taagacggtt actattgaca tgtatgaacc ctatgtccga ttatttcgcg acctatttcc
      781 taatgcagct attatttttg acagattcca tatcgttcaa catttaaata gagaacttaa
      841 taagtatcgt gtacaagtta tgaatgaata ccgtaataaa aaaggacctg attatacaat
      901 ttttaagaat aactggaaag tcctattgat ggatactagt aaaaccatat ttagtaaata
      961 cagatggaat aaatctttta aggcttataa acgctcatct gacattgtag aattcatgct
     1021 ttcaaaagac gatatactac gacactccta cgaacttgtc caaggattac gaaaagacct
     1081 aaggttatgt aattggccta aatttattaa tcgtttaaat tcagttagta aaaagtctgt
     1141 gagtaagggt gtatggaaag tggttaaata ttatagaaaa catcaaagga tgttaagaaa
     1201 tacaatttat tacccagcat ttaataatgg tgctatagaa ggaattaata ataagataaa
     1261 attaatcaag tgaatttctt ttggttacag aaatttcaac aactttaaag cacgtataat
     1321 gatgattttc agcttgtaca aaggagaaaa aagaagacaa ccaagcccaa taatggactg
     1381 gccgcctaat aataaaaact ctaaaagttg tattttaaaa atagttcttt aaattatata
     1441 cccaccacat ttggtggaga accaaaagaa accttgtttt acaaggtttc taatacgtta
     1501 tgttatgtaa ataacagtta attataccgg tggtcggggt cgaaccgaca ctccacaagt
     1561 ggaacgggat tttgagtccc gcgcgtctgc caattccgcc acaccggctt aatggtaaac
     1621 aaaaaacttc cctttggaag caattatgga gcggaagata ggatttacac ctatacctcg
     1681 ttccgggaag gaacgtgttc taaaagttga actactcccg caaatattaa attatggagc
     1741 ggaagatagg atttacacct atacctcatt ccaggaagga atgtattcta agagttgaaa
     1801 tactcccgca ttattattaa attatggagc ggaagatagg atttgcacct atacctcgtt
     1861 ccgggaagga acgtgttcta aaagttgaac tactcccgca taaacctgga ggcggcaacc
     1921 ggatttgaac cggtgataaa ggttttgcag acctctgcct taccacttgg ctatgccgcc
     1981 aataactggg ctagctggat tcgaaccaac gagtgacgga gtcaaagtcc gttgccttac
     2041 cgcttggcta tagcccatta ataataaggg cggctgaagg ggatcgaacc ctcgaatgtc
     2101 ggaaccacaa tccgatgtgt taaccacttc accacagccg ccatggcagg ggcagtagga
     2161 atcgaaccca caccaaaggt tttggagacc tctattctac cgttgaacta tgcccctatt
     2221 aaaaataata aatggagggg ggcagattcg aactgccgaa cccgaaggag cggatttaca
     2281 gtccgccgcg tttagccact tcgctacccc tccataaatg gtgccggcca gaggacttga
     2341 acccccaacc tactgattac aagtcagttg ctctaccaat tgagctaggc cggctaagaa
     2401 atggttcagg acagagtcga actgccgaca catggagctt caatccattg ctctaccaac
     2461 tgagctactg aaccataata aaaatgtaat gatggcggtc tcgacgggaa tcgaacccgc
     2521 gatctcctgc gtgacaggca ggcgtgttaa ccgctacact acgagaccta taaaatattg
     2581 cgggaggcgg atttgaacca ccgaccttcg ggttatgagc ccgacgagct accgaactgc
     2641 tccatcccgc gataataaaa aataatggcg gaggaagagg ga
//
LOCUS       DAKTGS010000202         2551 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.209, whole genome shotgun sequence.
ACCESSION   DAKTGS010000202 DAKTGS010000000
VERSION     DAKTGS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.209"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..794
                     /locus_tag="O0286_002737"
     CDS             <1..794
                     /locus_tag="O0286_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCY0171049.1"
                     /translation="KKIKVDNVRDIRAIYDKLVSNEINEQDKLDGELFRKNFVGVHDG
                     STNKYIHVGLQPETKIVEFIGEMLTFLKYFDAPQPFKIMASHYLFEYIHPFYDGNGRV
                     GRFIIAKLLSDYYDNYTALTFSYVINRNKSKYYKAFMTASNHLNCGDLTEFIDTMLEL
                     LIAGQERILDELIPKMDATEKLTLYLTSHYKQIDYEFLYLLSMDKLFGNKRNRLTLID
                     LENILGVGRVKINNTIKKYDNYLVKIKSRPTIYEISDEFLNSIIK"
     gene            1208..1960
                     /locus_tag="O0286_002738"
     CDS             1208..1960
                     /locus_tag="O0286_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCY0171050.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(2162..2431)
                     /locus_tag="O0286_002739"
     CDS             complement(2162..2431)
                     /locus_tag="O0286_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171051.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
BASE COUNT          986 a          270 c          423 g          872 t
ORIGIN      
        1 ataaaaaaat taaagttgat aatgtaagag acattagagc gatttatgat aaattagttt
       61 caaatgaaat taacgaacaa gataagttag atggagagct atttcgtaaa aattttgtcg
      121 gtgtgcatga tgggtcaacg aataaatata tacatgttgg gttacaacct gaaaccaaaa
      181 ttgttgaatt tataggtgaa atgctaacat ttttaaaata ttttgatgcg cctcagccgt
      241 tcaaaatcat ggctagtcat tatctgtttg aatatataca tcctttttat gatggcaatg
      301 gtagagttgg aagatttata attgccaagc tcttgagtga ttattatgat aattatactg
      361 ctttgacatt ttcatatgtt ataaatagaa ataaatcgaa atattacaag gcgtttatga
      421 ctgcttcaaa tcatttgaat tgtggtgatt tgacggaatt tattgacaca atgcttgagc
      481 tattgatagc aggacaagaa agaatacttg atgagttaat accaaaaatg gatgcgactg
      541 aaaaattaac gctttattta actagtcatt ataaacagat agattatgaa tttttgtatc
      601 tcctttcaat ggataaattg tttggaaata aaagaaatag attaacatta attgatttag
      661 aaaacatatt gggagtagga agagtaaaaa ttaataatac aattaaaaaa tatgataact
      721 acttagttaa aattaaaagt agaccaacaa tttatgagat aagtgatgaa tttctaaatt
      781 cgattataaa ataaatataa tatgtgatca gctatactta tagtatttgt aagatcgcgt
      841 aattgcttca ttgaaaaaat atatttgttg agagtggtgc atgacaaatg tactgctctt
      901 tttgttgtgt atcagtattg tgatgtttta atgagaatat tatatgaatc attatgaaat
      961 ttaataaaaa taaaagaaat gattatcatt ttttcttata tactgttaaa cggtttggaa
     1021 tttttaggta tacactgtat tggttgatat aactcaacta ataattgcga acagagtatt
     1081 tcaaattgaa aagtattatg agcgtgatac ataatcaaaa ttgtaggctc aagaaccact
     1141 acataataaa ccataagcgg ttctttatca tttatgtctc gctctcaaat gtaaattaat
     1201 aattgttttg ggggagtttg aagttaaata tttaacagga tttattttaa tattattgtt
     1261 agaaggaatt tttacaaatt cagcgagtgc aatcgaatat tcagacttac atcataaaag
     1321 taagtttgat tcaaagcgtc taagtaatgc taagatgtca ttcatcaatc caactcagct
     1381 tgaaaataaa aacacaaacg atagactgtt gaagcatgat ttgttatttc atgacatgtt
     1441 cgtaaatgat gattggaaaa aggattttaa agttgaattt gaaaatgagg cactttcaaa
     1501 gaaatttata aataaggata tcgatatatt tgctggaaat tatggatacg gatgtcatgg
     1561 gggagcaacc aataaaacgc aatgtagtta tggtggtgtt actttaagtg acaataataa
     1621 atacgatgat tataagaata taccttgtaa tttatggatt gacggacatc aaacagaaat
     1681 agaactaact gcagtaaaaa cgaaaaagaa aattgttact attcaagaat tagaggttca
     1741 attaagaaat tatttgaatg agaagtataa gttgtacgaa caaggtggcg acattgttaa
     1801 agggtatgtt aaatattata atgatgatga acaaaatgta gaatatgatt tttataattt
     1861 aaatggtgag tatggtcgtg aggtattaaa aatgtatgct gataataaaa ctatcaatag
     1921 tgacaaattg catttagata tctatttatt caaatcataa tttaattaag ttaatcatta
     1981 ataatctttg acagtgaaat ataaagtacc tgtcattatg ttgttacaaa taaaaacagc
     2041 agtaaggatg ctaacactta gaaaagtctt actgctgttt tctattagaa ttatctttaa
     2101 tgcacaaagg aattcgatac gaaacaaata tgattaactg tgtaattaca aaataaaact
     2161 actatagtaa aaagaagata aagacgatga taataaatgc atcgaatata attttcttga
     2221 tgtttttatc aacaacatca ggtaaggaca taagtataaa tcctagaatt agagtgtagc
     2281 gacatatgtt cataattgtt gctgtaaact cgatattaaa tgaaaacttt aaaaatatac
     2341 ttataaataa aatgacaaac gacgcaattg caataaaact gccgtatgta gctatctttg
     2401 ttacgagact attttgatta ttttcagcca ttgtaaaatc ctcttatttc atcattatta
     2461 ttttaaacat atcattttcc tgttatttaa taaagtttct tgacacgatt ggttgatgta
     2521 attgatatga ttagtttttg aaaaatagac a
//
LOCUS       DAKTGS010000203         2527 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.210, whole genome shotgun sequence.
ACCESSION   DAKTGS010000203 DAKTGS010000000
VERSION     DAKTGS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.210"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            182..255
                     /locus_tag="O0286_002740"
     tRNA            182..255
                     /locus_tag="O0286_002740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:216..218,aa:Arg,seq:tct)
     gene            complement(730..918)
                     /locus_tag="O0286_002741"
     CDS             complement(730..918)
                     /locus_tag="O0286_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0171052.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            1167..2114
                     /locus_tag="O0286_002742"
     CDS             1167..2114
                     /locus_tag="O0286_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCY0171053.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
BASE COUNT          802 a          428 c          341 g          956 t
ORIGIN      
        1 tacagttagg cattcaaaac tgtatttaac acaattaagt tgcctgaatt cgtatttaag
       61 tcttattgaa cctttttaga taaatagctc tataatagtg aaaaatataa acatttttta
      121 tttacaaggt attgctaatt taagttcatt tagatataat aattcttgtg ttgttaaacg
      181 tgtcctggta gctcagctgg atagagcaat ggccttctaa gccatcggtc gggggttcga
      241 atccctccca ggacgtttat aggtattttt atacgcatta ccaaacaaaa gagttccgtg
      301 attacggggc tctttttgtt ttgaatttca gtaatatagt atgatgcgtc accaaaacgt
      361 cccccgcata agccccgaaa atacagtaat taaaacaagc atgcttattc gttatagaat
      421 tttttgacac acaattgaca cgcgtctgac acttgtttat acatttttaa ttaagtaatt
      481 ttgtgctcaa atttcatcta tactgcacct gaactacacc aacactacac caagattttt
      541 aacactcacc atttgcatgc gtagagattt ttattattat attattccta tagattttga
      601 tactattcaa aattttaggg acttttcagg ggcccgaaat cctataatta taattatata
      661 catctaaaaa aaataaccac gtccatcgag acgtggtttt ttaattattt aactcactaa
      721 ttattcttct taattttcat tgctaaatat actattggca aatttataaa gtagttcagc
      781 gtttttcaat gacatattgt ctaatgatct ttcatttttt ctcattctgt gtattgtgct
      841 ttgtggaact cctgtttgtt tcgatatatg taaactgctc aaatcactgt ctaatagttt
      901 ttgaatttga tttctcattt tgtacacccc cttgtaaatc ttcaatcatc atttaaatta
      961 aataattact tttccacata ttccaatatt taggttgcaa agcatacctc aaatatcatt
     1021 aattttgaga tttaaatgtc aattatgtgt ctatcaatcc aatatacata ctctaataac
     1081 gtaatagtac acactcttct tattaattgg ccatagctat catgatataa ttagtgaaga
     1141 aaatcacata agaaaggttg taaatcatga gacttcaaaa agcacctcta gtaacgtcag
     1201 gactagtctt aggattatta ggcctgggta atctattaaa agacttatct cttactttaa
     1261 acgctgtttg cggaatcttt gctttcttga tttggattca ccttttatgt actatgatca
     1321 aatattttaa taatgtgaaa gaacaattaa acagtcctct agtttcatca gtgttcacaa
     1381 catttttcat gtctggcttt ttaggtacta cttatttaaa tacatttttt agtaacataa
     1441 cttttatcaa tagcttaata acgcctattt ggattttatg ccttgtggga attatgacgc
     1501 atatgattat tttttcaata aaatatttaa aagatttttc acttaaaaat gtttatcctt
     1561 cgtggactgt actttttatt ggtattgcta tcgcaggatt gacggcaccc gttagcggat
     1621 attttttcat aggtcaatta acagtaatat atggctttgt agctacttgt attgtcttac
     1681 ctatagtttt caagcgatta aaagcatttc cattgcagac gtcaatcaaa ccgaacacat
     1741 cgacaatttg tgcaccattt tctttagtcg ctgcagcata tgttatagct tttcctaagg
     1801 cgaatgcttt tatcgtaatt atatttttac tattagctca aatattttat ttttatatca
     1861 ttatacaatt gcctaaatta ctaaaagaac ctttttcgcc cgtattttca gctttcacat
     1921 tccctttagt aatctcagca actgctttaa agaacagttt gcctgtactt atgtttccag
     1981 acatttggaa aggtcttttg tttatcgaag tgttattagc cactgtaata gtacttagag
     2041 tctttatagg atatcttcac ttctttttaa aaaaggaaaa acaagataaa tttcttcgta
     2101 atgcgtctca gtaacactat taccaagaat taacacgtat atttaataac taaaaagagg
     2161 caggtaatct tgcctctttt tagtgtatat caatgcctct ttgatttaat atgatcttat
     2221 caatgatacc ttctgactaa agctatagta actatactag aacgtctagt tcataaacac
     2281 gtttcactct aaactttatt taaataatat ataagcagca aacgccaagg ctaatatacc
     2341 aataaaataa ttaataaaga tttttatttt agtctttgta atagttgaca aattttatca
     2401 cctctaaata tcgataaatt aaacaccgtt atttttcctt tgcctatctt ttatcatttc
     2461 agaaggtact ccgaaaataa acatcagaat aaataatata attggcaaat gtggtatgtt
     2521 ttctagc
//
LOCUS       DAKTGS010000204         2492 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.211, whole genome shotgun sequence.
ACCESSION   DAKTGS010000204 DAKTGS010000000
VERSION     DAKTGS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.211"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            20..661
                     /locus_tag="O0286_002743"
     CDS             20..661
                     /locus_tag="O0286_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0171054.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNC
                     VIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIFPSFKNLCFVLFSYFFCGG
                     LSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            749..1366
                     /locus_tag="O0286_002744"
     CDS             749..1366
                     /locus_tag="O0286_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0171055.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            1441..2070
                     /locus_tag="O0286_002745"
     CDS             1441..2070
                     /locus_tag="O0286_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0171056.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            2127..2243
                     /locus_tag="O0286_002746"
     CDS             2127..2243
                     /locus_tag="O0286_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171057.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            2266..>2492
                     /locus_tag="O0286_002747"
     CDS             2266..>2492
                     /locus_tag="O0286_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0171058.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAG"
BASE COUNT          922 a          285 c          386 g          899 t
ORIGIN      
        1 taaaaccaac gagacggaag tggagatatt gctttgcgag gttagagttg taaacaaaaa
       61 tcccagatat agaattatta aatataaaaa cgactatctg atgattgatt tagtaagcac
      121 ctggttagtt ttgttctttc cgtttattaa ttggttaata ccaaaaaaat atgtccaaat
      181 cagcagagaa gattttgata atttaaatat tgtcaaacca gttaaaaata aagctttatg
      241 gccagctata ggtagcatac ttttatttgg cactatgttt agagataaaa tatatatacc
      301 tgattctcat ttagaaaaaa attgcgttat tatcatttgt tcggttttat tattaagtat
      361 tctagtgttt tatatatatt taaatcagaa agtaaagtta tctatctata atgatcggag
      421 tagcaatgga aaaattatga tttttccatc attcaagaat ctttgctttg tactattttc
      481 ttattttttc tgtggtggat tatcaatcat gttcttagat gttttgatta gtttatctat
      541 ccaaaatatt atagtattta ttgcttgggt tattatgaca atgctatttt tctttataaa
      601 tatgtcttca ataatagaca aaaagattca tgttatatat ttaaggtcat ataaatatta
      661 attttgatac aaaagggcac aagtgttttg agaaacagaa acgataatat agctgtgtga
      721 atttgttatt aataggaagt gataattatt gctttgtgaa accgaaatta tcaataaaaa
      781 tccgaaatac agagttatta agtatgatga tgagtattta atggtcgatg taataagaac
      841 ttggcttgta tacttttttc catttattaa ttggttcatt ccaaaaaggt gcgccaaaat
      901 tagtagagaa gagtatgaaa aactgaacac tgttaagcca gttaaaaata aaaatttttg
      961 gcctgttgta ggaggaacaa tcctactagg tgctacttcc agaaagtaca tacacttact
     1021 taatattcaa ttagaaaaaa gatcggttat atttatatgc ttcgttgtat ttctatgtat
     1081 tttaatcttt tttgtgcttc tgaatcgaaa attaaaattg aaagttttcg ataataaaaa
     1141 agaagaacaa aaaataattt tagtaccaac attaaaaaat gctgttctta tattatatgg
     1201 ctatttgttg attggaggca tgtcaatatt agctttaagc atgttgctga cattagaaaa
     1261 ccagaattta ataactttta tagcttgggg tatggggtta atgttgtttt tcttgatgaa
     1321 tataacactc attgtcaata aaacagtcaa agttataaaa aggtgaataa ataactaatt
     1381 atattgtatt ggccgttctt caaataaaaa ataatttgaa taaggtggaa gtgataaaca
     1441 gtgctttgcg aatctagagt cattaatcaa aaccctaaat atagaattat taaatacaat
     1501 aatgaatatt tcatggttga tttagtaagt acttggattg cttatttttt gcccatgatt
     1561 aattggttta ttcccaaaaa gtacgcgaaa attagtagag aagaatttga aagtttaaat
     1621 attgtcaaac ccgctaaaaa taatactttc tggcctgttg caggatttgc agtgttatta
     1681 acaaccttaa caagaaaata tatctatttg cttaacatcc atttagaaaa agaaatagtt
     1741 atattaacat gctgtatgat acttctaggt gttttcgcat tgtttatata tataaataca
     1801 aaattgaagt tacatatttt tgataaaaat aaaagtaata acgaaaagat catattaata
     1861 cctacattta aaaatatttg tttatcctta tttgcttata tattatttgg tggattgtca
     1921 acaatggctc tgagtatgtt agtaacttca tcccctcaaa atataataga atttcttgct
     1981 ttaattggca tgactgcatg cttctttcta ctgaatatgt catcggttct agataaaaaa
     2041 attcatgtta ttttaaaaac aaataagtag taaaattgat taacttaggt agtatcggat
     2101 acttaaatgt tggttcataa aaagcaatga ttttaaatcg aggagctatc ttagaacagg
     2161 gaaataaaac agccaaagtt ataaaaagtg aattaataac taattatatt atgttagcca
     2221 cgcttcaaat aaaaaataat tagaataagg tgggattgat aatcaatgct atgcgaatct
     2281 aaaatcatca ataaaaaccc caaatataga attattaaat ataatgatga gtacttaatg
     2341 atcgatataa taagcacttg gattagttta ttttttcctt ttattaattg gttcatcccc
     2401 aaaagatacg tcaaaattag tagagaagag tttgaaaatt taaatattgt gaaacctgct
     2461 aaaaagaatg ttttttggcc agttgcaggt at
//
LOCUS       DAKTGS010000205         2488 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.212, whole genome shotgun sequence.
ACCESSION   DAKTGS010000205 DAKTGS010000000
VERSION     DAKTGS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.212"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(117..677)
                     /locus_tag="O0286_002748"
     CDS             complement(117..677)
                     /locus_tag="O0286_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCY0171059.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(697..>2488)
                     /gene="kdpB"
                     /locus_tag="O0286_002749"
     CDS             complement(697..>2488)
                     /gene="kdpB"
                     /locus_tag="O0286_002749"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCY0171060.1"
                     /translation="NFSEAFAEGRGKAQADSLRQAQSNLTARLIEENGAYRIVNATEL
                     KAGQNIRVENGETIPADGVVINGLATVDESAITGESAPVIKESGGDFDGVIGGTLVTS
                     DWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIALFTLLTTLTIIFLVVIVTLYPI
                     ASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGMDRVTQFNVLAKSGRAVEVCGD
                     VDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAAYMSSIYDDTPEGKSIVRLAKQ
                     MYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGAPNSMINLVKQQQGNIPLNIES
                     LCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVERFTELRKMGIETVMCTGDNAL
                     TAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIVAMTGDGTNDAPALAQANIGLA
                     MNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMTRGALTTFSLANDVAKYFAILP
                     ALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIVALIPIAMKGVKVKGYSIDRIF
                     INNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
BASE COUNT          751 a          524 c          363 g          850 t
ORIGIN      
        1 catgctaatt tattcttcct aaaggtacat ctaaaaattt aattaagcag aaagtgcttg
       61 aattgctaaa aagacaccat gtataatttt atcaacatga tgcctttcat ctataatcaa
      121 tctttcatct tatcaagagc gatatttagt tcaagcacat tcacataatc atttgttaac
      181 acaccacgct gcttacgatg ttgaatcaag tcggccactc ttgaagtaga tacatgacga
      241 gcatcagcaa tacgaggtgc ttgcttcaat gcattttcga ccgtaatatg cggatctaag
      301 cccgacccag aacttgttgc agcatctatt gttacatttg aattcccaaa tttaacatga
      361 tgtttcatgc gtgctattaa ttcggtgttt ccattcgatt cattacttcc acctgaagat
      421 acgccgtttt tatataattt ttcaggattc atattataat caactgcact cggtctcccg
      481 tgaaaatatc gtgtctctgt ccagtgctgt ccaatcaatt ttgatccaac tatacgattg
      541 tcatacgtaa ttaaactgcc atttgcttgt tgataaaaaa atatttgacc aattaacgtg
      601 atagctaacg ggaataaaaa tccacataat accatagtta ttatcgttaa acaaatacta
      661 tttcttatcg tattcatggt acaggctcct tcctctttac acaaaaaatt gtacaatcat
      721 atctattaat ttaatgccta aaaacgggac gattaatcca cctaatccat aaatcaacat
      781 attatttata aagattctat caatgctgta accctttact tttacacctt tcatggcaat
      841 tggaattaag gcaacaatga ttaatgcatt gaatatcaaa gctgatataa ttgcagattt
      901 aggtgacgat aaatgcataa tatttagtga agtcatttct ggaattgttg acatcattag
      961 tgcaggtaaa attgcaaagt attttgctac gtcattagcc aaactaaatg tcgttaatgc
     1021 acctctcgtc attaataatt gtttgcctat ttttacaacc tctattaact ttgtaggatt
     1081 cgaatctaaa tcaattagat tagctgcctc tttagcacta attgtccctg agttcatagc
     1141 taatcctata ttcgcttgtg ctagcgcagg tgcatcattt gtaccatctc ctgtcatcgc
     1201 aacaatatgg cctttcgctt gttcatcttt gatgacttta attttatctt cgggtttaca
     1261 ctctgcaaca aatctatcaa ccccggcttc ttttgcaatt gtagctgctg ttaaagcatt
     1321 atcacctgta cacataactg tttcaatccc catttttctc aattcagtaa atcgttctac
     1381 aagaccatct ttaatcacat cttttaaata aatcacgcca agcatgacat tgttttcaat
     1441 gactattaat ggtgtgccac ctttactcga tacatccata cagagagact caatattaag
     1501 aggaatattg ccttgttgtt gtttgacaag atttatcata ctattaggtg cacctttgaa
     1561 taccgatatt tcatttgtaa tgattccgct cattctagtt tcagctgtaa aaggcttata
     1621 tgtgccatca atgtctttag gcagctcatt tatatacatc tgcttcgcta atcgtacaat
     1681 actttttcct tctggcgtat catcgtagat tgatgacata taagcagcga ctatcaattt
     1741 ttcaagcatt tgttgattca ctggtaaaaa ttcactagcg attcgattgc cataagtgat
     1801 tgtgcctgtc ttgtctaaaa tcattacatc gacatctcca catacttcta cagcacgccc
     1861 acttttcgct aatacattga attgagtaac acgatccatg cctgcaatac caatcgccga
     1921 taacaaacca ccgattgtcg ttggtattaa acatactgtt aacgcaatga gcatcgcaat
     1981 aggtaaaatt aaatgcaggt aagatgctat tggatataac gttacaataa cgactaaaaa
     2041 tataattgtt aacgttgtta ataatgtaaa aagtgcaatt tcatttggtg ttttatttct
     2101 ttccgcccct tcaactaagg caatcatttt atctaaaaaa gatgtacccg cttcactctc
     2161 aacacgtatt tctaaccaat cagatgttac aagtgtaccg ccaatgactc catcaaaatc
     2221 gccacctgat tcttttatca caggtgcaga ctcaccagta attgcagatt catcaacggt
     2281 tgctaatcca tttattacaa cgccatcagc agggattgtt tctccatttt ctacccgaat
     2341 attttgtccg gcttttaact ctgtggcgtt cactatccga tacgcaccat tttcttctat
     2401 caatcgagca gttaaatttg attgtgcttg tcttaaacta tcagcttgcg cttttccacg
     2461 accttcagca aaggcttctg aaaaatta
//
LOCUS       DAKTGS010000206         2432 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.213, whole genome shotgun sequence.
ACCESSION   DAKTGS010000206 DAKTGS010000000
VERSION     DAKTGS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.213"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            64..717
                     /locus_tag="O0286_002750"
     CDS             64..717
                     /locus_tag="O0286_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0171061.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
     gene            751..1338
                     /locus_tag="O0286_002751"
     CDS             751..1338
                     /locus_tag="O0286_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0171062.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(1481..2374)
                     /locus_tag="O0286_002752"
     CDS             complement(1481..2374)
                     /locus_tag="O0286_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0171063.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          726 a          305 c          455 g          946 t
ORIGIN      
        1 tggcgaagaa gatttattta acttataaaa atattgaagt aagattgggg agattatgaa
       61 tttatggaat tgaaagtcga tgattttgta aagaatataa aaagaccata cttgactgta
      121 ttgggagtat ttgtagttgc agtttcatta ttttttgacg cgattatgtt tttctacgct
      181 aaactatatg ataaattgcc tatgtattta ctggtgttta tggcctttac agctgtaatt
      241 ttgattatga tgtacataca agagaaaaat gaaaattaca aagttgaaaa aagatatgtg
      301 gttagatatc tcacacttaa cgttattgtg ggatatactt tgccattgct ttttgtatct
      361 atttacgttt ttggtgtagt cggttttgga tttgatgttt tcaattattg tctaggtatt
      421 atcttgatgt tatttatttc ttggttaggt ttatttttat tttataaaaa cgaatttgat
      481 agtgaaaatc ctaatcctgc agttaatgcc atagcaatta ttataaaatt atttgctttt
      541 ggaggaatat tttatataag tctaatagtt cctgtaacac agcaagaaga gatttttata
      601 gggttaagta tattcatcaa tataattgtt gatgctcttc ttgttaggtc atattttaat
      661 tacgcgttat ataagagtat taagaaagat atcgaaaatg aaggtaagac aatgtaaagg
      721 aaagacataa aaaataatag gagtctaacc atgataattt ttattttaat aacaatattt
      781 gctatttatt atatagctat gattgctagt ttgtttaaaa gtgaaggttt ttcaataata
      841 ggtttaatat tagatgttgt tataatgggg acattgattt tttactattt tataggcgct
      901 cgctttgttg atcatgattt aagcaacttt ttaatgttta tggatactgg atcatatatt
      961 tttatgtact ttgcaattaa gtgtttatgg gtgaaaccta aggtagtaaa ttatttgatt
     1021 gcaaaagaat tgggtgaatc taaagaggtc attgaagagc aggaattaga tttacagaca
     1081 tcaaagataa gaggtatcta ctttttcatt atttcagtag tgatgttaat tatcactaaa
     1141 ttaagaatgc aacctgagtt acaggcagat gctatatcga tgaatcctgt atttattttt
     1201 gttggtgtta taatcatttt aatttggcta gtactggata tatatcgcaa gaaaaaatac
     1261 ggtatattct tattcaagac gatagtgcca ctagttgtta cgacttggat tattatagct
     1321 acaattatac tttcgtaaac gattttgatt aatggggaat gtagagaaca tttctttgta
     1381 attttataat cgaataacat acaaaataaa gcgaccaatt ttgcagtgtt acgaattgta
     1441 aactgcgaac tggtcgcttt ttattgatgt catgattaat ttaatggatg taattatatg
     1501 atgaaacttc tgaagcagag atggttcttg atgaaacgat atattcacca atccagttca
     1561 tttctgaaat tagaatactt ccatcaatat taactttttc aacgtaggct acatgaccaa
     1621 atggaccatt tactgtttgt aaaattgatc ctcgtgttgg gtgtctatct actttgaagc
     1681 cattgcttga agcttggcct gcccagtttt tagcatctcc ccaaaatgta ctaatcgtgt
     1741 gtccatcttt ggcacgttta tcaaagacat accatgtaca ttgtccagca gtatataagt
     1801 tgttcttact tgtgataaga ggctgatcaa tgattttacc gttacctaat gctaaaggtt
     1861 taccgtcagc ggtctttgct ttgtcattaa attcggcgat ttgtaattcg tcatacaatt
     1921 cgtctaaatc gatgcctgta ataagccctt tgttatcttt tgaaaaagcg ttatttaatt
     1981 catcgtcatt gtcttcgaca ttcggtattg ctggtgtcaa aggattgctt ggtgacgttt
     2041 gaggcggtgt gtgtgaatca attgcgtcat taatgtgcgt atactgacca cttaatgaag
     2101 aatggtactg attgttgtta agatcacgtt gatttgcgtg ttgatgattg tcgtttgtac
     2161 gtgactggtt ttgatgattg ttgtttggcg tgttgttttt gtcatatgta taagtatacg
     2221 cgccggtgtc tttattcact ttgaactgtg cgtttgggtg tgctttcttt gcttcttcta
     2281 atgttttgct atcattcgta tatgcttgag ccgagttagg cgacatacta aataaagtaa
     2341 gagttgtcat cgtcagtaaa attgttttct tcataataac catttaatcc tttatgtatt
     2401 taatttaatt ttagtataca catttatatt ac
//
LOCUS       DAKTGS010000207         2354 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.214, whole genome shotgun sequence.
ACCESSION   DAKTGS010000207 DAKTGS010000000
VERSION     DAKTGS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.214"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(194..1393)
                     /locus_tag="O0286_002753"
     CDS             complement(194..1393)
                     /locus_tag="O0286_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0171064.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
                     EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRF
                     KDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFN
                     NEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRA
                     FVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKG
                     LLQKMFI"
     gene            complement(1386..>2354)
                     /locus_tag="O0286_002754"
     CDS             complement(1386..>2354)
                     /locus_tag="O0286_002754"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCY0171065.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          662 a          445 c          334 g          913 t
ORIGIN      
        1 tctcttgtca taataaaatc gcctaccctc ttagtttaac aatgtctatt ctcatagaat
       61 ttgtccaact aaatgtagac gattcactaa acttctaaaa aaataaaccc caaattataa
      121 tagtctcaat attaatatat tacaatttat tcataggatt tttatcattt caattaatag
      181 gttgttatca gggttaaata aacatttttt gtaatagtcc ttttttacgt tcttttaata
      241 actcaattct attcatttga ttattcattt tattgtctat agattttaac aatgcactta
      301 ctttatcttg ttcagtgaga caaggtattt taagatttat attgcataag ttcgcatgag
      361 ttaatttagc tggtgcatta cctgtgacaa atgctcgtag ttctttaaaa tttaaataat
      421 aattcataaa aaacaaatta tgatcattac ttttaactac atgcgcatga ttatttaccc
      481 agtattgccc attagcaata aagctactcg tctcaaactg cccccatttt gcaccatctt
      541 ctcctattag taatcgttct tcattattga ataaataatc ttttacgtaa tcaataattc
      601 cagttgcacc atagtaaggg tataacccct tttctcttaa tgaagaagta attggttttc
      661 ttctattatt ttcaaagata aagatgtctt ttatgaattt gttttcccac tctggatatt
      721 cttcaccatt ctcatctttg aatcgcaatt cttgtgtgaa gattttctgc atatagcctt
      781 ttttctgttg ttgaagcaat tcaagttttt gttcttctaa ttcaatttgt cggtcgagtt
      841 tgctgaagaa cttgcctatt ttttgctgtt cttcaaatat agttggggtg aagattttta
      901 aattagcaat ttctttgaag tttagacctt ctcgactacc tccactttgt gcaaggaaaa
      961 ttttcctttt accttttctt gataatagat actgtccaaa aaaattataa taatactctt
     1021 ttttcaatct aataatacat acatgttgat ttaaattagc atgcgtttca actatcgaat
     1081 taatggctgt tctacctatt gatgctcctg taatatttaa aagaacatca ccatagtacg
     1141 ttctactatt tttcatctca tcatctatat ctttactaat ataaactaag tcattaagat
     1201 ttaatttacc atttctaata ttttgactcc ttaaaaatgg tatgcctttg tttgtatagt
     1261 tttcacttcc acctttggga gtctttccac tacctatttt ggtagtaaga ttccctaact
     1321 tcttctcttc ccattcgcct tcaaatcctg ggaatctcaa ttctggcaca tttttctttt
     1381 gtgtattact catctttcaa caccccaagt tctttcaggt atgcattgat ttcttgctca
     1441 atttctgcga tttctttatc gatatttttc aaatcttgtt ggacttgatc taaatcaatc
     1501 ggtgcttctt cttcgaatgt atcgacatat ctcggtatat ttaggttgta atcgttatcg
     1561 gcaatctctt gtagtgtcgc gctgtagcta tatttatcaa ttgtttcctt acgcttatat
     1621 gtgtctataa tacgttcgac ttgggcatcg cttaaatgat tttgattttt tcctttttca
     1681 aaatcattgg atgcatcgat aaatagtacg ttgtcgtctt gttggcgaca ttttttaaat
     1741 actaaaatac atgttggaat acttgtccca tagaaaatat tcgctggcaa accaatcaca
     1801 gcttctaagt agttcttttc ttcaattaaa taacgacgaa tgacaccttc tgcagcacct
     1861 cggaataata caccatgtgg gagtacaacg gccatggtac cttcatcgtc taggtaatgt
     1921 accatgtgtt gaataaaggc aaagtctgct ttagacttag gcgcaagttt gccgtaacca
     1981 ctgaatcgtt cgtcattttc aaactttgaa tctgcagtcc atttcgcact atacggtggg
     2041 ttcgcaataa ccgcatcaaa tgtattgcct aaaaaggctg ggttttccaa tgtgtcatca
     2101 ttacggatat cgaagttctc ataacgcaca tcatgtaata acatattcat gcgtgctaag
     2161 ttgtatgtag tattgttacg ttcttgaccg aaataacgat acacttgtgt ttctttacca
     2221 acacgtaaca acagtgaacc tgaaccacat gttgggtcat acacgtgacg taatttatct
     2281 ttaccgtctg tgacaatctt cgccagtatc ttagatactt gttgtggtgt atagaactcg
     2341 cctgcttttt tacc
//
LOCUS       DAKTGS010000208         2318 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.215, whole genome shotgun sequence.
ACCESSION   DAKTGS010000208 DAKTGS010000000
VERSION     DAKTGS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.215"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..1162)
                     /locus_tag="O0286_002755"
     CDS             complement(<1..1162)
                     /locus_tag="O0286_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0171066.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGN"
     gene            complement(1155..1322)
                     /locus_tag="O0286_002756"
     CDS             complement(1155..1322)
                     /locus_tag="O0286_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171067.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(1297..2148)
                     /locus_tag="O0286_002757"
     CDS             complement(1297..2148)
                     /locus_tag="O0286_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0171068.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          839 a          383 c          297 g          799 t
ORIGIN      
        1 cattaccata gaaacgattg tatcgacgtt taaataattg gaataaatga gtaactaata
       61 cttttaaaat agcatatccc ggaataccta aaataacgcc tacaatacca agcaatttgc
      121 ctgcacacag taaaatgaaa atgattgtaa gtggatgaat cttaagtgtt ttacccatga
      181 tatttggtga aatgaagtgc ccttcaacaa attgtactaa agtccatact actgctaatt
      241 ttaagagcat ccacggcgat gttatagcag ctattacaat agctggagaa atcgctatag
      301 taggccctaa atatggtaca acacttgtaa ctgccgcaat actagctaat actaagctat
      361 atttcaaccc gataaccgaa taaccgataa acaacagtat accgatacag aatgaaacga
      421 taatttgtcc ttgaatgtat gaaccaactt gaacactcat tttttcaagt agatcatgaa
      481 aatcttttcg gaatttcggt ggcataatat tcgttgaaaa ttctttgaag tgatgtccat
      541 ctttaagcat aaagaatagt acaaatggtg ttgtgacaat cacaacgcca atattagcaa
      601 tcgtttctgc aaacgttgca attttagacc caaagccatc tgtaaattca cttaacatag
      661 atggtatttt cttaggtaaa gaatttaacc aatcattaat ttgactataa tacgaagaga
      721 aaaacgtatt atttgctatt ttatcaatag aattaattaa tttttctaga tattgcggtg
      781 aatttttaac taaactatct acttgcgaac caataatagg tatcaataaa ttaacaatta
      841 acgtaataac acctactaca gctagataaa taatagaaat acctgcaact cttggtattc
      901 tataacgttc catcatattt acgattgggt taaatagata gaatagaatt aaagagacga
      961 tgataggtgc tgctatcgtc ttaaaaacga tgataaaagg atcaaaaaca tatgatactt
     1021 tttcgaaaat aaaaataaca atacccaaca atactagcgc tattaatgag aagactaaat
     1081 cattcccacc aacaaacttc atataccgtg attcggtaaa ttttaaaaaa ttctttctta
     1141 tattcttttc attttcattc aattatagat tcaccaccac ttaaaatatt cataagatgt
     1201 tgtataaaaa cagtccactt atgtaaaatt actaacatca atgataccat acaaataccc
     1261 tttacagtaa cgaaacgccc gtgtaataca cttaatttag gattgattaa tcctttcttc
     1321 aaaatgatga atgtgtaatg ttaaatatat aatttcagac tcactgatat taacatcaaa
     1381 ttgtttttgt atcatagtta aaattttata tgctgtgtta tagcaaatcg gatagtgatt
     1441 tttaatcata gacacaaaat catcttgtgc atgtatatat tcttttcttc ttaatcggcg
     1501 aattaaaaat tgtacgtgcc ttataaaacg ttggtattgt aatgattcct tatcaacagt
     1561 tgtcacaagg tctgattcaa tgatatctat acctttttta ataacattat tgatcaaggt
     1621 catctcatgc atagataaat cttctgtatt agatgcaata tgtaatgcaa taaatcctat
     1681 ctcatcttca ggaaaatgta catctaatgc tgcatttaac tgatcaatca cctgtttagc
     1741 aatatgatat gcatcactat ataactgcat agtttccata acaaatggat tgctaataac
     1801 ttgattttgt tttaagcgtt tataagcaaa tataatatga tccgttaatg aaactacaag
     1861 ttgttttgaa tcaacattca tcgcagtatt agaaataaaa ttcaacgaat caataattac
     1921 ttgtaataca ttatcatcag cgatttcaac taaactttta taatgtgctt tttgttgctc
     1981 actttctaat ttataaattt tctctattgt aatagtttgg tcgtttaacg ccattccctc
     2041 ttttttgtta aaaccaatac ctttaccgat taaaataact tcttgatcat tattagtaca
     2101 tactacgaca ttgttgttca atgttttagt aacaatatat tctcccatta tcatcaccta
     2161 tttttctatt attaagatta tatatcggaa atgtctaact tgaaagtata ataatttaaa
     2221 tacttgtgtt ctattacacg cctatcctat atgatatatc ttaatttaat ttcgatgtct
     2281 ctcaaagtgg aataactata ataaaaaaat ctgactcc
//
LOCUS       DAKTGS010000209         2159 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.216, whole genome shotgun sequence.
ACCESSION   DAKTGS010000209 DAKTGS010000000
VERSION     DAKTGS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.216"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            290..1690
                     /locus_tag="O0286_002758"
     CDS             290..1690
                     /locus_tag="O0286_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0171069.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
BASE COUNT          724 a          305 c          386 g          744 t
ORIGIN      
        1 catttaaata cgatgtcgaa aatggcgata gcatatcact tacatgaagt tatgtgctat
       61 cgctattttt agttataatt ccaaaaagtt aatcgttcga tgatttaaga attattattg
      121 tttaattcaa atgtatgagg gtataaaatc attgaattta attcgataaa gcgaaatttt
      181 tgaacaaaca tacttttgta tttatataaa agtttaaatt cttataaatt tgacaaaact
      241 aattaactcc gtataattat gaaacataca agagggagtg tatgaattca tggattttaa
      301 taaagagaat attaacatgg tggatgcaaa gaaagctaaa aaaaccgttg ttgcaaccgg
      361 tatcggtaat gcaatggaat ggttcgattt tggtgtctat gcatatacaa ctgcgtacat
      421 tggagcgaac ttcttctctc cagtagagaa tgcagacatt cgacaaatgt tgactttcgc
      481 agcattagcc attgcgtttt tattaagacc aattggtggt gtcgtatttg gtattattgg
      541 tgacaaatat ggacgtaaag ttgtattaac atctacaatt attttaatgg cattttcaac
      601 attaaccatt ggattattgc caagctatga tcaaattgga ctttgggcac caatactatt
      661 attgcttgca agagtactac aagggttttc aacaggtgga gagtatgcgg gggcaatgac
      721 atatgttgcc gaatcatctc cagataagcg tcgtaactca ttaggtagtg gactagaaat
      781 tgggacatta tcaggttaca tagctgcttc aattatgatt gctgtattaa cattcttttt
      841 aacagatgaa caaatggcat catttggttg gagaatccca ttcttactcg gtttattcct
      901 aggattattc ggcttatatt tacgtcgtaa gctggaagaa tcaccagttt tcgaaaatga
      961 tgttgcaaca caaccagaaa gagataacat taacttttta caaatcatca gattttatta
     1021 caaagatata tttgtatgtt ttgtagctgt tgtattcttc aatgttacaa actatatggt
     1081 aactgcatat ttaccaacct atttagaaca agttattaaa ttagatgcaa cgacaacaag
     1141 tgtattaatt acttgtgtca tggcaataat gattccatta gcattaatgt ttggtaagtt
     1201 agcggataaa ataggtgaaa agaaagtatt tctaattggt actggtgggc taacattatt
     1261 cagtatcatc gcatttatgt tattacattc acaatcattt gttgtaatag taatcggtat
     1321 atttatatta ggatttttct tatcaactta cgaagcgaca atgccagggt cgttaccaac
     1381 gatgttttac agtcatataa gatatcgaac tttatcagta acatttaata tctctgtttc
     1441 gatatttggt ggtacgacgc cattagttgc aacatggtta gttacgaaaa ctggagatcc
     1501 attagcacct gcgtattatt taacagcaat cagtgttatt ggctttttag ttattacatt
     1561 cttacattta agtacagcag gaaaatctct aaaaggttcg tatccaaatg tagataacga
     1621 gcaagataga gcttattatg cagaacatcc aaaagaagca ttatggtggg ttaaagaacg
     1681 taagaattag agattttaat aaaaagtata aatcaatcgt atataagcac tttaaagcta
     1741 gtaggttctg ctaactttaa agtgcttttt aaattgagaa ctgtaattag ccgtaataaa
     1801 gtttttgtat atacataaac ccccactgca atgattatcg caatggggga aagaggggac
     1861 ttaaagcata tgtttagctt tgaatactta aaattctctt gctattgaaa tgttaggatg
     1921 taaatatgtc ttagagtatt ttgtccaacg caattaatat tgagactcta accttcaata
     1981 ttattataga gaacacaaac ttaaatagat tgggtgactt atttgtgtca gttattgcga
     2041 ttgcgataac ttcttttctc tatatacata tagtaacgtc ttatctaata aaaaacatgg
     2101 tactacagta tcaaatttat ctagggctta agtttgattt ttataatagg caggtttac
//
LOCUS       DAKTGS010000210         2123 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.218, whole genome shotgun sequence.
ACCESSION   DAKTGS010000210 DAKTGS010000000
VERSION     DAKTGS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.218"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            70..840
                     /gene="fabI"
                     /locus_tag="O0286_002759"
     CDS             70..840
                     /gene="fabI"
                     /locus_tag="O0286_002759"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCY0171070.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(1035..2120)
                     /locus_tag="O0286_002760"
     CDS             complement(1035..2120)
                     /locus_tag="O0286_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0171071.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
BASE COUNT          766 a          306 c          375 g          676 t
ORIGIN      
        1 aaaaagttag ttaaaactgg gattagatat tctatccgtt aaattaatta ttataaggag
       61 ttatcttaca tgttaaatct tgaaaacaaa acatatgtca tcatgggaat cgctaataag
      121 cgtagtattg cttttggtgt cgctaaagtt ttagatcaat taggtgctaa attagtattt
      181 acttaccgta aagaacgtag ccgtaaagag cttgaaaaat tattagaaca attaaatcaa
      241 ccagaagcgc acttatatca aattgatgtt caaagcgatg aagaggttat taatggtttt
      301 gagcaaattg gtaaagatgt tggcaatatt gatggtgtat atcattcaat cgcatttgct
      361 aatatggaag acttacgcgg acgcttttct gaaacttcac gtgaaggctt cttgttagct
      421 caagacatta gttcttactc attaacaatt gtggctcatg aagctaaaaa attaatgcca
      481 gaaggtggta gcattgttgc aacaacatat ttaggtggcg aattcgcagt tcaaaactat
      541 aatgtgatgg gtgttgctaa agcgagctta gaagcaaatg ttaaatattt agcattagac
      601 ttaggtccag ataatattcg cgttaatgca atttcagctg gtccaatccg tacattaagt
      661 gcaaaaggtg tgggtggttt caatacaatt cttaaagaaa tcgaagagcg tgcaccttta
      721 aaacgtaatg ttgatcaagt agaagtaggt aaaactgcgg cttacttatt aagtgattta
      781 tcaagtggcg ttacaggtga aaatattcat gtagatagcg gattccacgc aattaaataa
      841 tatcattcaa cagctttgtt cacgttatta tatatgtgag caaagctttt ttgcttttat
      901 aataatcgga ctgatggaaa attatttgat atttcatttg actgattttt tatgcacaaa
      961 aaaatcgcct caaaagctgt agtttcaact tttgatgcga tagtatctta tattcttaaa
     1021 agacatccac agaattagtc cttaacatta ctgtttgctt tatcaataat gcgttggcgg
     1081 tatttgaaaa tattactaac aaccgtttta agtacagcat ataatggcac tgcaatcaga
     1141 actaatgtaa agccacctaa atctcctgct gctaaaataa caacgataat tgttaaagga
     1201 tggatactta aagatttacc cattacattt ggtgtaatga tattaccttc aagttgatgt
     1261 gcaattaatg taatgataca aacccatata aatgtagtag gactatctat aataccgagt
     1321 attgctgcag gtgcaaatga taaccatgaa cctaagaaag gaattaagtt tgcgacacca
     1381 gcaaatagta ctaataaagg aatatatggt aagtcaataa ttgaataacc gatatataag
     1441 aatataccta aaataacact gacagttact tgaccttgaa tgtaagattt taatgtaaag
     1501 tttaaatcag ttaataaatc tacgaaaaat actttacgtt cacctttgaa aaatttagca
     1561 acagctggga taaatttttc atggtctttt aacatataaa ttaagaagaa tggaaccata
     1621 atcaataaga agatggttga aattaatgat gtaatgtact gtaatgaatt agataaaata
     1681 ttagtaacgc catcacccat tgatttaacc atatttgtaa ttctacttgt tacatcttca
     1741 ggtaatttat ccatttgagc tagtgcgaat ttaataattt gctctgcttc tttttgtagg
     1801 gcaggtgtct gactaattaa attgttgata tttgaaatga tgattggtgc aacaaacgca
     1861 acaattaagc cgataatagc aaacaaagct aacatgattg ttgttatact agcccatctt
     1921 ggaaaaccga ctttttcaag taagttttga aaaggtagac agatataaaa taaaaaacca
     1981 ctaattaaaa atggaagaaa tacagaaccg atgattgtag ctattggagt aaatacttcg
     2041 tgcacttcca taaatagttt gatgagaatg aacagcataa tcagagcgat gccagttcgg
     2101 aaccaaacct tgtttaacat aaa
//
LOCUS       DAKTGS010000211         2110 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.219, whole genome shotgun sequence.
ACCESSION   DAKTGS010000211 DAKTGS010000000
VERSION     DAKTGS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.219"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            93..449
                     /locus_tag="O0286_002761"
     CDS             93..449
                     /locus_tag="O0286_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCY0171072.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            607..987
                     /gene="gcvH"
                     /locus_tag="O0286_002762"
     CDS             607..987
                     /gene="gcvH"
                     /locus_tag="O0286_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCY0171073.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            1166..2044
                     /locus_tag="O0286_002763"
     CDS             1166..2044
                     /locus_tag="O0286_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCY0171074.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
BASE COUNT          803 a          250 c          404 g          653 t
ORIGIN      
        1 atcataataa aaaattttag cttcaaaatg aaagcctttt gctttggaaa atgatatatt
       61 tattttaaat acataaagga ggttgcagtc gtatgattaa attttaccaa tataagaatt
      121 gtacaacttg taaaaaggca gcaaagtttt tagatgaata tggcgtaagt tatgaaccaa
      181 ttgatatcgt tcaacataca cctacaataa atgaatttaa aacaataatt gcaaatacag
      241 gcgtagaaat taataaattg tttaatacac acggcgcgaa atatcgtgag cttgatttga
      301 aaaataaatt acaaacttta tcagatgatg aaaagttaga gttgttatca tctgatggta
      361 tgttagtaaa gcgtcctcta gcagtaatgg gcgataagat aacattagga tttaaagaag
      421 atcaatataa agagacttgg ttagcgtaag tgaaatgtaa gcgtttacta aatatctcga
      481 tatttagatt cattacatgt aaaatgaaat aagctataca attgttaatt tttataaata
      541 tagttgaata gcatctagcc ttatggcatc attaatgatg taaagattaa ttaggagggg
      601 attctcttgg cagtaccaaa tgaattgaaa tattcaaaag agcatgaatg ggttaaagtt
      661 gaaggtaatg tagcaataat tggaatcaca gaatacgcac aaagcgagtt aggtgatatt
      721 gttttcgttg aattaccaga aacagatgat gaaattaatg aaggggatac gtttggtagc
      781 gtagaatcag ttaaaactgt atcagaatta tatgcaccaa tctctggtaa agtagttgaa
      841 gtcaacgaag aactagaaga tagtcccgaa tttgtaaatg aatctccata cgaaaaagca
      901 tggatggtaa aagtagaaat tagtgatgaa agtcagattg aagctttatt aacagctgaa
      961 aaatattcag aaatgattgg tgaataatca ccgtgtaact ccttaatcta agattgagga
     1021 gtttattttt agtctgagaa aataaatgat atgaaagaaa aattattagg tactattatt
     1081 tggagtattg ctacatttta ttattcaaga atgatggaaa taatgaattt agctatttta
     1141 aaaataaaaa ttgggggaag ttaatatgct aaacattcaa gacgttagtc atctttctaa
     1201 aaaggagcaa aaagcatata accgtttcgt agaatctgta gaaaacggta atttaccagt
     1261 actaccatgt attgaaatgg atctaaaaga gatgcaagaa gaaacattaa accagagtaa
     1321 gattggtgga atgccatttt taaaatattt taaagatata ccattagatg aaaataatgt
     1381 accaatggta ttgctagcac agattaattt ggatgatctt ccagaacaac aagaattatt
     1441 tcctgtaaaa gaagggatat tgcagttttg gattagttca gaagatcaaa tgtatggaat
     1501 gtctgaaaat ttaaagggaa acaatataaa ctcaaggctt gtttatataa aagagccaat
     1561 tacagattta tcactcgaaa atattcaagc gcatttgaag tcattagatg ctgataatga
     1621 ggatatcccg ttcagtggag cattttctat agaatttaga ttgtcgaaac aaactattac
     1681 atgtactgat tataagtacg atgaggacgt gcttgcattg tggaataaag tcaatccatc
     1741 cttcgcgcta aaatcaatgt ttggtggtta tgatgaattg atggaacctg tgtgtaacac
     1801 atttactgct aaggaaccat ttaatcaact tggtggttat ccatattttg accaaataga
     1861 tccaagaacg aacgatcaag aactgaaaat gtatgataga gtcttactgc aaattgattc
     1921 tacaagagat ggtaattctt cgattatatg gggtgattta ggtattgcca atatcttagt
     1981 gaaatctact gaccttgagg ctatgaagtt tgatgattac atgtattcat gggattgcag
     2041 ctaagagaat cataaaaata tattacttta ataagtgtag tatctataga tcaatctagg
     2101 gagtgggaca
//
LOCUS       DAKTGS010000212         2085 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.220, whole genome shotgun sequence.
ACCESSION   DAKTGS010000212 DAKTGS010000000
VERSION     DAKTGS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.220"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            40..747
                     /gene="deoD"
                     /locus_tag="O0286_002764"
     CDS             40..747
                     /gene="deoD"
                     /locus_tag="O0286_002764"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0171075.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            754..>2085
                     /gene="tet(38)"
                     /locus_tag="O0286_002765"
     CDS             754..>2085
                     /gene="tet(38)"
                     /locus_tag="O0286_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0171076.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNR"
BASE COUNT          639 a          318 c          386 g          742 t
ORIGIN      
        1 atagacaaat cactatacaa atattgggag gaatatttta tgaaatcaac accacacatt
       61 aaaccaatga atgacgtcga aattgcagaa acggttctat tgccaggaga tccgttaaga
      121 gctaagttca ttgcagaaac ttatttggat gatgtggaac agttcaatac agtgcgaaac
      181 atgtttggtt ttaccggaac atataaaggt aaaaaagttt ctgtcatggg ttcaggtatg
      241 ggtatgccat ctattggcat ttactcttat gaattaattc atacatttgg ttgtaaaaaa
      301 ttaattcgcg ttggctcttg tggcgcgatg caagaaaaca ttgatttata tgatgtgatt
      361 attgcacaag gtgcctctac tgattcaaat tacgttcaac aatatcaatt accaggtcat
      421 tttgcgccaa ttgcttctta tcaattatta gaaaaagcag ttgaaacagc acgtgacaaa
      481 ggtgtacgtc atcatgtagg taatgtgtta tcaagtgata ttttctataa cgcggataca
      541 acagcgagtg aacgttggat gcgtatgggt attttaggtg tagaaatgga atcagctgca
      601 ttatacatga atgcaattta cgctggtgtc gaagcattag gtgtgttcac agtgagcgat
      661 catttaattc atgaaacgtc aacaacacct gaggaaaggg aacgtgcatt tacagatatg
      721 attgaaattg cactgtcatt ggtgtagatg attatgaatg ttgaatattc taaaataaag
      781 aaagcagtac ctattttatt attcttattt gtattcagtt tggttataga caactcattt
      841 aaattgattt ctgtagccat tgctgatgac ttaaacatat ctgtaacgac agtaagttgg
      901 caagcgacat tagccggttt agtaattggt attggcgctg tagtatacgc ttcattatct
      961 gatgccatta gtatacgcac actatttatt tatggcgtga tattaatcat tatcggatca
     1021 attattggtt acattttcca acatcaattc ccattacttt tagttggacg tattattcaa
     1081 actgccggtt tagctgctgc agagacatta tatgtgatat atgttgcaaa gtatctttct
     1141 aaagaggacc agaagactta ccttggctta agtacgagca gttattcctt gtcattagtt
     1201 atcggtacat tatcaggtgg atttatttct acgtatttac actggacaaa tatgttttta
     1261 attgcattaa tcgtagtatt tacgttgcca ttcctattta aattattacc aaaagaaaat
     1321 aatacgaata aagctcattt agattttgtt ggcttaattc tagtggcaac tattgctaca
     1381 acagtcatgc tgtttattac gaactttaat tggttatata tgattggtgc cttaattgcg
     1441 attatcgttt ttgcgctata tattaaaaat gcgcaacgtc cattagtaaa taaatcattt
     1501 ttccaaaata aacgttatgc ttcattttta tttatagtat ttgtaatgta tgctatccaa
     1561 ttgggttata tttttacgtt cccattcata atggagcaaa tttatcatct gcaactagac
     1621 acaacatcac tgttattagt accgggttat atagtagcag tcattgttgg tgcactaagt
     1681 ggtaaaatcg gcgaatatct gaattcaaaa caagcgatta tcacagcaat tattttaata
     1741 gcactgagct tgattttacc tgcatttgca gtaggtaatc acatttcaat cttcgtcatt
     1801 tctatgatat tctttgcagg tagctttgct ttaatgtatg cacctttact taacgaagcc
     1861 attaaaacaa tagatcttaa tatgacaggt gtggctattg gtttttataa tttaattatt
     1921 aatgtggcgg tatctgtagg tattgcgatt gctgcggctc taatcgattt taaagcatta
     1981 aatttcccag gcaatgatgc attaagttca catttcggta ttattttaat tattttaggt
     2041 ttaatgagta ttgtcggatt agttttattc gtcagcttaa atcgt
//
LOCUS       DAKTGS010000213         2051 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.221, whole genome shotgun sequence.
ACCESSION   DAKTGS010000213 DAKTGS010000000
VERSION     DAKTGS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.221"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            643..1209
                     /locus_tag="O0286_002766"
     CDS             643..1209
                     /locus_tag="O0286_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0171077.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
     gene            complement(1683..>2051)
                     /locus_tag="O0286_002767"
     CDS             complement(1683..>2051)
                     /locus_tag="O0286_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCY0171078.1"
                     /translation="SINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMA
                     IVVGVLMFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITL
                     LLAVLLFKGFNKWSGRTIKD"
BASE COUNT          761 a          315 c          269 g          706 t
ORIGIN      
        1 atgtattgtg tttaacaaca ttattagcga ttagttcccc tattatttat gacaatatgc
       61 agtcaatcaa tgaaacatgt atgtatgagt atatttggaa ctagtaatgt attgttaaat
      121 attgaagatg attgaattat ggaatttttt tcgagatatg ttatttcggg gatcagattt
      181 tgcgattttg ccttggatag agtagttgcg atttgtaaat acgtttttca ggttcgattc
      241 aatttcgttg ttaagataat atggattagt ttcactgaca tgttgatgca tgtctttttt
      301 gttctgataa agtataaaat gtttacttaa cattacttag tatgacacct gctaattcaa
      361 acattatttg agacattctt ttcaaattaa ttataaattt ttacctatag actagtttga
      421 tatttatcta catctcaaaa ttctcatcaa caatctttca catccaacat ttttacttta
      481 gtttttataa ttcaaaacaa caaaactatg ttaaaaaatt attctatttt ttagttaata
      541 gatagttaat acatttttga tatttagtta attgttcttt taaaaaatta ttattatatt
      601 ttcattgtaa acgtttacaa tataaaaaaa ggagcaataa aaatgaatac aaaattttta
      661 ggtaaaacat tagtagcaag tgctttagta ttaacaacat taggaacagg cttacattct
      721 tcatacttag gattagatac aaataaagtt gttaaaacgg ctaaagcaga agaaaaaatg
      781 acagatggtc aattatggaa aaaagttaaa gattcattac atgattctga tattatttta
      841 agtaacgagt acgaaacaat caacgttact tatttattaa gcaatggata ttctagcagc
      901 gtttctgctc ctggaaatga tgatggaggc catctaacac aatctataga ctttaaagga
      961 ttaaaacaaa ttgatttaac taaggaaaat gtctacgatg actttaacaa aaagctagat
     1021 gctaaaaata catggaattc tctaaccgag aagttaaaag gtcttggttt actacaaaat
     1081 ggccaaaaag tatctattta ttcttcagat agctcttctc cagtctcggg taaagtggga
     1141 gaaggagtaa ctagtggtgg cgaaaatact ctaactaaac gcttcatcaa caaaatcaca
     1201 attgattaaa ccttaaactc aaatttcctt aattcataaa acattaataa ctaacccttt
     1261 cttacaaacc acctagccca taatatatta aaacgtcatt tttatttcaa ttagcggaaa
     1321 ataaaaaaac cattccgcat taactttggc aagttggaat ggttaaaaaa atattttaga
     1381 agccagcgtt tggcttgctt agacgacatg ttcgcgcatg tcgtcttatt tttacattta
     1441 tataataaca cgcaattcaa taaaatgtct aatcaataaa cctcaattac attcaaattc
     1501 aaaaactttt cacctaatat tcatcgctta aataaacatt acgtttaatt aaacagacat
     1561 tgtcaatcgc gtctaatacc cctgctgtcc gaaaacaaaa ttattttgta ttcatattta
     1621 actgttctct ttcctccctt ttcaacgcaa ctaaatttct tttaccgtta gagccccaat
     1681 ctttaatctt taattgttct accgctccat ttattaaatc ctttaaagag taaaactgct
     1741 aatagcaacg tgataataat atagattgcc aatgttaatg taactggtat actcccttcg
     1801 ataaacatat aaacgtaacg tgtagcatat gtgattggta aatagaacca cgaatgatct
     1861 ccaagcactt ctaatccaaa ataaacgtta aaaataaaca ttaaaactcc gacaacaata
     1921 gccattacat ctttaatgaa aatactaaaa ataaaaagta gcagtaatat aattacattg
     1981 aaaaacaatg atacgcctat aaacataagt gttattttca tatcatgtga atgccacaat
     2041 aaattaattg a
//
LOCUS       DAKTGS010000214         2037 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.222, whole genome shotgun sequence.
ACCESSION   DAKTGS010000214 DAKTGS010000000
VERSION     DAKTGS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.222"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(145..789)
                     /locus_tag="O0286_002768"
     CDS             complement(145..789)
                     /locus_tag="O0286_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCY0171079.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            1150..1947
                     /locus_tag="O0286_002769"
     CDS             1150..1947
                     /locus_tag="O0286_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCY0171080.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          755 a          338 c          288 g          656 t
ORIGIN      
        1 caattaaggt aatatatttt tattgcttta tcagtaaaat ctcagtctaa gtttctaact
       61 tatgacaatc gctccagttt aaggttgata ttcattattg attattccaa aactttatac
      121 aaatttcttt attaatttaa ctgtttatct tctgaacata ttagcaaggt aaagtatatc
      181 cttaataata ttcaacaaat taataaatag attaaatccc atttctcttg gtgaatagtc
      241 acctcttttt aaacgattaa aatcatacaa agtataaagt agaaataaca acagactaac
      301 gacggtaata atagtgtaaa aaataggatt ttgaagaaaa ataccaatta gactcgcaat
      361 aattaatgtt attaatgtaa cgaataaata tttgcctata ctcgatgcat ctccaacgaa
      421 gaaataacca ataataccaa atgctataaa tgcaaaaatt gctaatgcga tatttttata
      481 gaaaatatct ggtcctaaat tttgtaaata cgtggtaaac gttgcgtatg acaataagcc
      541 aatcactaca gcgtaaatat gtgaaataat taaaccgaac cgtctcgctc tttcaaaaac
      601 aagtgttgct aaaataataa tcagtaatcc aaatgacaag ggttgtcgcc aacttaatgg
      661 taaaaactga cctagatagc atcctatgcc aaatataatc caataataca taaaataaag
      721 ccacacttta cttattgatt gagcatgctg gttttgatga taatatgaat gatttgtatt
      781 ttgcgacaaa ataattcctc cttctaccgt cgttttatgc gtcaaatttt acagtaaatt
      841 ttgcttcatc aaaagaaata acctttaaca agtatagtaa ttttacattt tacaatgtta
      901 caaaatataa ttttttataa ttagttaaaa tcactaaaac gcttttatac actatcaaat
      961 cagcatttat aaaaatatga accgatatcc taaaatgtta ataatattac aagataataa
     1021 caaaccacac aaagctactt atttttgata atatggaaat cgtaatataa aacgaaaact
     1081 taatttacta tataaattgt cttaataatt tttaaaagta gtaaaacata attttaagga
     1141 ggagtccctt tgaaaaaatt agcatttgca ataacagcaa catctggtgc agctgcattt
     1201 ttaacgcatc atgatgcaca agcttctaca caacatacag tacaatctgg tgaatcatta
     1261 tggagtattg ctcaaaaata caacacttca gtagagagta ttaaacaaaa taaccaatta
     1321 gataacaact tggtattccc tggtcaagtt atctcagtag gtggaagtga tgcacaaaat
     1381 acgtcaaaca cttctccaca agctggttca gcatcatctc atactgtaca agctggtgaa
     1441 tcattaaata tcattgctag cagatatggt gtttcagttg atcaattaat ggcagccaat
     1501 aacttacgtg gttatttaat tatgcctaac caaacattac aaattcctaa tggtggatca
     1561 ggtggtacaa caccaacagc tacaacaggt agcaatggca atgcatcatc ttttaatcac
     1621 caaaatttat acactgctgg tcaatgtaca tggtacgtat ttgaccgtcg tgctcaagct
     1681 ggtagtccaa ttagcacata ttggtcagac gctaagtatt gggctggtaa cgcagctaat
     1741 gatggttacc aagtaaacaa cacaccatca gttggttcaa ttatgcaaag cacacctggt
     1801 ccatatggtc atgttgctta tgttgaacgt gtcaatggtg atggtagtat cttgatttct
     1861 gaaatgaatt acacatatgg tccatacaat atgaactacc gtacaattcc agcttcagaa
     1921 gtttctagct atgcattcat ccattaatta aataaattgt actgatatat actagcaatt
     1981 cacatcatgt gagattgcta gttttttatt tttgaaaaaa attttcattt tggtaca
//
LOCUS       DAKTGS010000215         1992 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.223, whole genome shotgun sequence.
ACCESSION   DAKTGS010000215 DAKTGS010000000
VERSION     DAKTGS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.223"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            157..882
                     /gene="sasD"
                     /locus_tag="O0286_002770"
     CDS             157..882
                     /gene="sasD"
                     /locus_tag="O0286_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCY0171081.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(1074..1829)
                     /locus_tag="O0286_002771"
     CDS             complement(1074..1829)
                     /locus_tag="O0286_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0171082.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
BASE COUNT          700 a          319 c          339 g          634 t
ORIGIN      
        1 attgacatta aataaattga caaaataagt aattattgtg aatctaaagt gaaattttta
       61 taaaaaaatg taatgattca aaaattttgt tgcatttctt ttgtaatcgt atgataatgt
      121 aaatgtaatc aaattgtaat ataaggggac aagacaatga aaaaattagc aacagtaggt
      181 tctttaattg taacaagcac tttagtattc tcaagtatgc cttttcaaaa tgcgcatgcc
      241 gacacaactt caatgaatgt gtcgaataaa caaagccaaa atgtacaaaa tcatcgtcct
      301 tatggcggag tagtaccaca aggaatgacg caagcacaat atactgaatt agagaaagct
      361 ttaccccaat taagcgctgg cagtaatatg caagactata atatgaaatt gtatgatgcg
      421 acgcaaaata ttgctgataa atacaatgtg ataattacaa ctaatgtagg ggtatttaaa
      481 ccacatgctg ttagagatat gaatggccat gcgttacctt taacaaaaga tggcaatttt
      541 tatcaaacga atgtagatgc aaatggtgtt aatcatggtg gtagtgaaat ggtgcaaaat
      601 aaaacaggtc atatgagtca acaaggccat atgaatcaga acacacacat gaaccaacag
      661 ccacacatgc aacaaggtca tatgcaatca tcaaaccatc aaatgatgag tccaaaagca
      721 aatatgcatt catcaaatca tcaaatgaac caaagtaaca aaaaagtttt accagctgct
      781 ggtgaaagta tgacatcaag tattcttact gcaagtattg ccgcactact attagtatct
      841 gggttattct tagcatttag acgacgttca acaaataaat aaacataata cgattaataa
      901 tagaaaaatc gtgtgattat ctgagcgagc ctaggacata aatcaatgtc ctaggctcgc
      961 taatgttata ttggcagtag ttgactgaat gaaattgcgc ttgtaacaag cttttccatt
     1021 tcttaccaac tccctaaata gtcatcaaaa aatttcttat attttaataa tttttaataa
     1081 tccgattgtc ttatacgtgt cagtgttaat tcagatattt cctgtggaat ataccactta
     1141 ttaatcataa ttggataagg tgtttgtgcg tacagtgttt caataatcag ccaacaatgt
     1201 gtatcaccat caaacacgtg actatgattt ttgaagtggg gcgctttggt aatagacatt
     1261 tttaaatctg attgatatgc attgctataa atcgtttgct caacgaatgt cttcatgtcg
     1321 tcttcgtttt gtgtattcac tttaaatgtg tcaatgacat ttaacggtat aaaggtaaag
     1381 caaaatgcat cagcttgctt agaatgattg tccttttttt gataatagcg ttccattgca
     1441 atgacggcag aaggatggtt tgcaaacaaa tgatttgtat attcactttc taaatcaaca
     1501 cgataattaa ttgatgacat agatacgcga gctagcaata tttgatcaag tggatgctta
     1561 aattgatcca tacttgaagc gtgttgggca tttgtttgtg gaataacaaa gtgtcccttc
     1621 cctcttgtac tctctacgat gccatcttcg gctaacaatt ttatagcttg gcgcaaagtc
     1681 atacgactga catcaaagcg cgcacaaagt tccttttcag taggtaatgc atggccactc
     1741 ggatattttc ctatttgaat ttctttatat aacgtattat aaatcgttaa aaattttggt
     1801 tgtgtttgcg tcacgtagac aacctccata aagttactta atcactctca tcatacaata
     1861 atttttactc aaattggaaa aattataaaa attaaatata gataggcttt gaaaattagt
     1921 tttatacaag gttagtagct gtaactgtaa aatgttctta atattgtcaa aatgtaatgc
     1981 ttgaaagcgc tt
//
LOCUS       DAKTGS010000216         1964 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.224, whole genome shotgun sequence.
ACCESSION   DAKTGS010000216 DAKTGS010000000
VERSION     DAKTGS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.224"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..110
                     /locus_tag="O0286_002772"
     CDS             <1..110
                     /locus_tag="O0286_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0171083.1"
                     /translation="RWGLFGYCRFIPYLKKRLNNKIVLMKYKVPKRQRH"
     gene            complement(482..1705)
                     /locus_tag="O0286_002773"
     CDS             complement(482..1705)
                     /locus_tag="O0286_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCY0171084.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
BASE COUNT          638 a          322 c          289 g          715 t
ORIGIN      
        1 atcgatgggg cttatttgga tattgtagat ttatacctta tttaaaaaaa aggttaaaca
       61 ataaaatagt acttatgaaa tataaagttc caaagagaca gagacattaa gttcttagac
      121 aatgtaaaaa agctgatttc tataaattat ttgatagaaa tcagcttttt tcaaatgtat
      181 ttgataattt acagctcgtt gagctgctgt tttctaggga tttatgtccc attcctttta
      241 cgagaaggtt tttacgagtt ttatgtaact attagaagct atctttttat atgaccatta
      301 gtcaatatat gtatgacaaa tcagcacaat aatttcagtt ttacatcact taatcaatga
      361 aaatgtgctt gttgatattt gaatatttac attcaaacaa tattcatcgc tatttctatc
      421 aacttaaaat tcgcaataaa aaactgccag cacgcgaata gggcagctga cagcatcgtt
      481 cttatttttc ttttaaatat tgaatggaag cctctttaaa aatatcaata tatttcagat
      541 acatatcttt ttcaatatat tcatcgattt gatgtgccat taatggatta cctggtccaa
      601 aaatggctaa atcaacattg tccttattat ctcctaagaa actagaggca tctgttgcac
      661 ctacaagcgc tgaaacaaat atttcgtctt gttctacata actagaagct acatctttaa
      721 tcgtagtaat taatttgcta tttttatcgc ttgttacagg tcggtgattg cttggaatat
      781 cgagtgaaag cttattgcta tccacatcat taatgatatt ttggaaaaac gattctataa
      841 agtcgttatc atactcagga actggtctta cgttaaattc aagtgaagct tcatctggta
      901 cagagttaaa ttgtttgccg ccatttataa tcgaacatac agctgtaaga ccagatgcat
      961 aatttgcatc ctcttcagaa atttcttttc caatcaatga tttgaacata ggcgcaacat
     1021 ctaattcatg tttagtatct tgttgtttaa gctctgaata tttttcttta aatagattat
     1081 aaaattcaag cagtgtatca attgcattgt caccaataaa tggaactgag ctatggacag
     1141 ctttaccagt tgcagttact ttacatgaca tagacccctt atgtgcataa taaattccag
     1201 atccagttgg ttcagcaata attaagctat cgacatcgtc taaatagcct ttatcagcta
     1261 ataatttggc accttcttgt tctttctctt cgccagcagt agccagtaat ctaatcgttc
     1321 catgaggcaa ttcattttgt tcttttaatt cgattagaga tacgaccaaa gccattaaac
     1381 cgcctttcat atctgtagtg cctcggccat ataatttgcc atctttttct gtcagttgaa
     1441 aagggggata tgaccaatta tcttgatttc ctgcatcaac aacatccata tgaccactca
     1501 atgcgagtat aggtgagccg ttaccgattt ctgcaacgat attggcgcgg tgttcattaa
     1561 ctttcaaaat ttcagattta atatcgtact tgtcgaataa atctgttaaa taattacaaa
     1621 cgtctatttc attattattt tcagtttgta gttcaacaat atctgctagt aattgaattt
     1681 tttctttttc actaaaagtt gtcattaagc tcacaccttt caaagtagta tatatattta
     1741 tataaacata atttttattc ttaaacataa tttaaaatgc agaaagtagt gtgttatttg
     1801 atttcgttct atgaaattaa ctgtctagta tcaacgatct tattaacgct gatatcggat
     1861 atgcttaatt ttatagtaaa ttgtatgaga aagatgcagg atattattta gctaataatt
     1921 atatataaat ttcaaatcga tggttattaa ctactaaata aaaa
//
LOCUS       DAKTGS010000217         1964 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.225, whole genome shotgun sequence.
ACCESSION   DAKTGS010000217 DAKTGS010000000
VERSION     DAKTGS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.225"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            193..1683
                     /locus_tag="O0286_002774"
     CDS             193..1683
                     /locus_tag="O0286_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCY0171085.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
BASE COUNT          798 a          191 c          384 g          591 t
ORIGIN      
        1 cgaaaatcgc gtgaaataat cagaggaatc gtttgaaatc atcgtagtaa tcgcgcgaaa
       61 atcgcgtgaa ataatcagag gaatcgtttg aaatcatcgt agtaatcgcg cgaaaatcgc
      121 gtgaaatact atggtagacg gtttgagtaa attaatggag tattttaata tttatggttt
      181 ggaggaattg cgttgcatag agaaagtaaa tatatagagt ataagaaatc acgaaaagga
      241 ttatctaatg atatttggtc tacgtatagt gcttttgcaa atactgaagg tggtactata
      301 tatttaggaa ttgaagaaaa aaagatcgag gacaaaaaag tctttgtttc agttggtgtt
      361 gaagatccag agaaaatgat tgaagatttt tggaatgcac tatatggaag aagtaaagtt
      421 agtcaaaata ttttatcaaa taaagatgtt aaaattgtta atattgaaaa taaagcgtgc
      481 attgaaattc atgtaccaga agcgccttat tcgaagaaac cgatatatgt agataataaa
      541 aaagatttag tatataaaag agttgatgat gctgatagaa ttgcgactga agaagagtat
      601 aaattcatga ttgtaaattc tcaagacgat atagatacag aattattaga taactatgac
      661 atgtctgatt taaatcacga atctatcgaa aattatagga aacttctatt aaaaaatact
      721 aatgatgaga gatatgcgaa tatgagccaa ctggatttaa tgatagattt aggagcatat
      781 agaaaagata gaagttcgaa agacaaacag tataaaatga ctacagcatg tttattattc
      841 tttggtaagt ataatgcgat tagtgataga ttcccaggat ttcaattaga ttattttaag
      901 aaaacaaatt acctagatac tgattggaaa gatagaatat caagtggaga tttaggtaat
      961 gaagatttaa acgtgtatag tttttttgaa aaagtattga taaaattaac tgataacatt
     1021 gaggaatcat ttagcctaaa tgatggtttg actagacaaa attatgcaag agatttaaaa
     1081 gtagcaattc gcgaagcact ggttaataca ttaatgcatg cgtattatga tactaagcaa
     1141 agtattaaaa tagttaattg tgaagatttt atagagtttt ataatccggg taatatgaga
     1201 ataaataaag aagattttat tcatggaggg cattcaaagg acagaaatag tatattatcg
     1261 acgcttttca gaagagtagg atattcagaa aaagctggat ctggaggacc aaggatattc
     1321 gatgtagtta atagacataa gcttaaaacg cctgaaatag aattaacgga catggacact
     1381 aatgtagtac tttggaaaca agatttaatg aaggagtttg aaaaatatcc tgagttagac
     1441 aaaaaagtaa taaagtatat tattgactat ggatcaataa gtaagggtga agccttaaaa
     1501 atggaaaata tgacagaata tcagtttaga aatattttaa aaaaactaaa agatgataac
     1561 ttgataaaaa aagaaggtga aggtccggct actaaatatg tgttaataga atcaaaagaa
     1621 gctgatatat tgcgaactaa aaaagtaatt aaaagtttag agtctttctt taggaataaa
     1681 taaaaaaaca agagataggt gcgaagtgtt tggattatac aaatgcttcg catcttatat
     1741 ttattaataa atatcataga aaaccgtatc attaaccgat acgcagagat gcggtttttt
     1801 agacacttca taaagggatt ttgaacgtat cagaacatat gaggtttata ggaattgctg
     1861 ttatgttttt tgatcacatc aataaacaaa aaaggtatgt actatgtaaa atatttatta
     1921 aatgatataa agcgagggta tataaatgat ttttaaatag atat
//
LOCUS       DAKTGS010000218         1955 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.226, whole genome shotgun sequence.
ACCESSION   DAKTGS010000218 DAKTGS010000000
VERSION     DAKTGS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.226"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            63..482
                     /locus_tag="O0286_002775"
     CDS             63..482
                     /locus_tag="O0286_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0171086.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(564..1916)
                     /locus_tag="O0286_002776"
     CDS             complement(564..1916)
                     /locus_tag="O0286_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCY0171087.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
BASE COUNT          690 a          285 c          317 g          663 t
ORIGIN      
        1 tgcgtatatc tataggctcg tagtatactt attaaaatat taaacaaaga aaggatttta
       61 agatgaatag aaaacctgaa ttaatcatgg cttggattgc aaatagtatt agtattattt
      121 atttattagt tatggggcta tcctattttt ctttaaaaag tggtaatgca tcacaacgtg
      181 aagaattagc gaagcaatta tctcagaacg gtggcaaggt ttctttagat atgcttcaga
      241 caacaatggg tgcattagca attattttat taatttcaac actttatggt atatttgcga
      301 caatttgtat taaaggacgt agaaaattat cgattatact ttttgttatc gcgataattg
      361 taagtttgat ggctcttaat ttaattgcaa ttgtcttatg ggttatcgtg atgattatgt
      421 tgatttctaa aaaagaatca aaagaaacaa cacataagga cgatgagtat atttatcatt
      481 aatatgttca tagcaaaaga aaaaccatta aaatgttaag ttgttaatta ttagatacaa
      541 ccaacatttt aatggtttta tttttaactt tgtagttctt ggaatgtttg aacgattaaa
      601 tagacattta aaatacttag aataataata agtgtccatg aaatgatatt aacccatgtt
      661 ttattataga aaggtcccat caagtcttta ttactagtag ccaattgtaa tggtattaaa
      721 cagaatggca gagcaatact taaaaatact tgagagaata caagtagttg ctcaatttta
      781 gctgcattac ctttaaaaat gattaagcat acgataacag gaatgacagc aagagaacgt
      841 gtaattaaac gtcttaacca atttggtatg tgtaatctta aaaatccttc cattacaatt
      901 tgtcctgcta aagtaccagt aatcgttgaa ttttgacctg acgctaataa tgcaactgca
      961 aataatgtac tcatgattgc acccattgtt gcacctagta caggttcagt ttttaaggcg
     1021 tgatataaat catagaaacc acctaaatcg tcagcatttg agttgaaaaa tagtgatgct
     1081 cctaacacta ataataagca attgactaca aatgcgattg ataactgaat gttcgaatct
     1141 atcgtagcaa atttaatcgc ttgcgctttt tcttcattgt tatgtcttga gtatgttcta
     1201 gattgtacaa ttgatgaatg taagtacaaa ttatgaggca taattgtagc gccaataata
     1261 cctaatgcaa tatagagaat gccgttattt gtaatgattt cactatgtgg tataaatcca
     1321 tttaacacag cattcaactg tggtgatgaa atatagactt caaatataaa gatgaataac
     1381 actgtgaaaa ttaatgtacc aacaatagct tcaatttttc taaaaccata tttcattata
     1441 aaaagtagta aaaatacatc aagtacagtt attagtgcac cgacgattaa aggtatgtta
     1501 aataggagat taagagcaat agcactacca ataacctcag caatatctgt agcgataatt
     1561 gctagttctg caatgatcca aaagattata gcaataggtc ttgataaata atgtcgtgtc
     1621 atttgagcta agtccatacc tgttgctatt cctaatctca ctgtcatgct ttgaagtaac
     1681 attgctgata aacttgaaat aagaattacg aatagcaaag tatagccata ttgggcgcca
     1741 ccttgcattg atgttatcca gtttccggga tccatgtaac caacagcgac taataaccca
     1801 ggtccaagaa atgataaaaa tttctgttta tttgaactgc gatgatcgaa tttaattgta
     1861 ttgtttattt cgtctaaact taattgttca tttgttgaat gtcgtttatt attcattttg
     1921 actcacctct acttcttatg gacaatataa taacc
//
LOCUS       DAKTGS010000219         1908 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.227, whole genome shotgun sequence.
ACCESSION   DAKTGS010000219 DAKTGS010000000
VERSION     DAKTGS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.227"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(137..1156)
                     /locus_tag="O0286_002777"
     CDS             complement(137..1156)
                     /locus_tag="O0286_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0171088.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(1230..>1908)
                     /locus_tag="O0286_002778"
     CDS             complement(1230..>1908)
                     /locus_tag="O0286_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0171089.1"
                     /translation="SLPYFFGTVGILYNKEKYPNESFDSWKSLYNPKFKNQILLVDGA
                     REIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAV
                     VWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNK
                     QNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFL
                     NFKMSLK"
BASE COUNT          590 a          350 c          252 g          716 t
ORIGIN      
        1 atagtaatga gaaatcgtca tttaaaatta tgacacctaa acactttaat taaacgttat
       61 cgataattaa cctcatataa aaacccagat taataagcaa aagacctctg cttactaatc
      121 tgggtatcat caatatttat tcttgctctt ttttgtcctt aacttcactt gaattgcgtt
      181 cttcagaaat atcattgttg taaatttgac gtcgctcttc aaaattcatt cgacgttgac
      241 taactgcgct aggttgattt ttcctacgtt ctttcattcg ttggttataa gctttacgtt
      301 tttctttttt cttagcttta atttcagctt tttctcgctt ttgttgtgct ttcaattctt
      361 ttggatcaac ttcaggttct gcataataac catcatcttc aatacctagt tcttcagtac
      421 ttttttgttt gtatttagcc tgtctggata atactgatgg ttcttcctca gtgcgctcat
      481 catgattata ttcactttca actgcacgtt taggaaattg atcatattta tctctttcct
      541 gttcttgatc atatacagta tttgatttat ccaattcttt ttcatattta ggcacatcat
      601 aataatcatc tttcggttgg gggcgctcat acacaggttc acgataatca taaatcggtc
      661 cgttatactc ttcataatat tgtggcgcaa ccatttcaac tttatctttt cttataaata
      721 gcatcatcgc aataataaag aataatatcg gaatgattaa agtaacgata agtaaaacta
      781 aaggaatcgt tacaattgct gatattaaga ataagaaacc tgacaagatt cttatattca
      841 tagatatgag agctaaaaat gaaatcaaca aacaaacaat aaagtaaacg ataatagccc
      901 atataccatt ttgtaaccat attacaaatt gtgttgtatt atagccattt ccagctaata
      961 attgctggat aaatgcatta ttgtttaatg tattttcaag attagcaatc gatgtgttat
     1021 tactgaatga aacaagtgct ataaacatcg taatgacagt aagcactaat aacatcaccc
     1081 aacataacca acctagaact ttttcagtta atctacttac tggacgttta atttgagtaa
     1141 attgttctcc agtcattcgt tacaactcct tatagtactt atcccgttat tataactaaa
     1201 tatacagtaa ataactacta tttatgattt tattttaatg acattttgaa attcaaaaag
     1261 ttttcattgt attcacttaa aacttcaggt cctaaatctt tataaacttc aaggcgttct
     1321 tgctctttct tagtcggata aaaacgatgg tcgtctttaa tctctttagg caacaattgt
     1381 cgagcagcct tgtttggcgt tgcatagcct acgaattctg tattttgctt gttattttta
     1441 gcatctaata aaaaattcat aaatttatat gcaccctctt tattttgtgc cgtttttgga
     1501 attaccatat tgtcgaacca taaattcgat ccttctttag gaataacata attatattta
     1561 tccccttctt gcactagagg tgctgcaaca ccactccaaa caaccgctat gttaccttca
     1621 ttttgttgaa gcatcatggt aatttcatca cctacgacac ctcttacttg tggtgctagt
     1681 ttggttaaat ctcgctctgc ttcttttaaa tggtgcgaat tacggtcatt aagattatac
     1741 ccaagtttat tcaaactcat gcctataatc tctctagcac cgtcaactag taaaatttgg
     1801 tttttaaatt taggattata caatgacttc caactatcaa atgattcatt tggatacttt
     1861 tctttattat ataaaatacc tacagttcca aagaaataag gtaaagaa
//
LOCUS       DAKTGS010000220         1881 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.228, whole genome shotgun sequence.
ACCESSION   DAKTGS010000220 DAKTGS010000000
VERSION     DAKTGS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.228"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            71..940
                     /locus_tag="O0286_002779"
     CDS             71..940
                     /locus_tag="O0286_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0171090.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            961..1527
                     /locus_tag="O0286_002780"
     CDS             961..1527
                     /locus_tag="O0286_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0171091.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT          743 a          232 c          334 g          572 t
ORIGIN      
        1 aaagtatgtg cgctgagtat atattcttat ttaagaacaa gattttaaat ttacgaaacg
       61 aggtaacata atgataaaac taatagccac tgatatggat ggcacgctac ttaatgcagc
      121 acatgaaatt tctcaaccta atattgatgc gattaaatac gctcaagaac aagggataac
      181 ggttgttatc gcgacaggtc gagcatttta tgaagcacaa gcaccagttg ctgacacaga
      241 tttaacagta ccatatattt gtttgaatgg tgctgaagta cgtgatgaaa ctttcaatgt
      301 aatgagcact tcacacctta ataaatcgtt agtacacaaa attacaaatg ttttaaaaga
      361 tgcaggtatt tattatcaag tatacacgag tcgtgcgatt tatactgaag atccacaaag
      421 agatttagac atttacatag atattgctga gcgtgcaggt caacatgcaa acgttgagcg
      481 tattaaaaat ggtattcaaa gacgcataga taatggtacg ttgaaagttg ttgataatta
      541 tgatgctatt gaaaacatac ctggtgaatt aattatgaaa atattagcat ttgatggaaa
      601 tttagaaaaa attgacaaag ctagtaaaat tttagctgaa tctccgaatt tagctatatc
      661 atcatcttcg agaggaaata tagaaataac gcattcagat gcacaaaaag gtattgcgct
      721 agaaacaatt gccgaaagat tagggattga aatgaaagaa gtcatgtcaa taggtgacaa
      781 tttaaatgac ttatcaatgt tagagaaagt tggctatcca gttgcgatgg aaaatggtgc
      841 agaagaagtt aaaaaaatag cgaaatatgt cacagatacg aatgaaaata gtggtgttgg
      901 aaaagctatt atgaaattat tacgtgaaca acaagtttaa taaaaaaaga ggggtcaaat
      961 atgaaaggat taattattat tggcagtgca caagtgaatt cacatacaag tgcactagca
     1021 agatacttaa ctgagcattt taaaacacat gatattgaag cggaaatatt cgatttagca
     1081 gaaaaaccgt taaatcaatt agatttttca ggaacaacac cgtctattga tgaaatcaaa
     1141 caaaatatga aagatttaaa agagaaagca atggcggcgg actttttaat attaggaacg
     1201 ccaaactatc atggttcata ttctggaata ttgaaaaatg cattagatca tctaaatatg
     1261 gattatttta aaatgaaacc tgtaggctta ataggaaata gtggtggtat tgttagttca
     1321 gagccattgt cacatttaag agtaatcgtc agaagtttac taggcattgc tgtaccaact
     1381 caaatagcaa cacatgattc tgattttgct aaaaatgaag atggttcata ttacttaaat
     1441 gatagtgaat tccaattacg agcaagatta tttgtcgatc aaattgtatc ttttgtgaat
     1501 aatagtccat atgaacattt aaaataatat taaaaaatat gtaaatatac attaaaaata
     1561 ggtatgtagc ttaaggtatt tcattgagaa tgcttttaag tcacatgcct attttttgtt
     1621 ctaatgcacc aatagtaagc ctttgatatt actgttgtga gcaatgttat taattaaaat
     1681 aagatgtaat agcgaattga aaacaagctt agattaaaag tgagttttaa aaatatagat
     1741 aaataattta aagcaattaa agaaaaaaag tattaaaaat tgcaattctt aaactgtgat
     1801 tcatattaat atttctagca aataacattg taaaataaag aaaaataatt aaagtattgc
     1861 actttattga aatttatatt a
//
LOCUS       DAKTGS010000221         1874 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.229, whole genome shotgun sequence.
ACCESSION   DAKTGS010000221 DAKTGS010000000
VERSION     DAKTGS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.229"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            165..1574
                     /locus_tag="O0286_002781"
     CDS             165..1574
                     /locus_tag="O0286_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0171092.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            1578..>1874
                     /locus_tag="O0286_002782"
     CDS             1578..>1874
                     /locus_tag="O0286_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0171093.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKI"
BASE COUNT          702 a          231 c          373 g          568 t
ORIGIN      
        1 ataagaaaaa aggcgacatg ttatctcaat ttacctgcat ctagactcct ttacataaga
       61 aataatcatc gccgagtttt aaaattttac aactttgtag atgtaaaaag catttgattt
      121 taaatagttt gaaaatcata tatttttgaa aaaggagcat gccgatgagt attgacatgt
      181 atttagacag atctcgaaac caagcttcaa gtgtggggaa tttgagtcaa acaatgaatt
      241 caaattatga tgcgttggaa aaagcaatta ctcaatttat taatgatgat gcgcttaaag
      301 ggaaagcgta tacgtcagct aagcaatttt ttagtacggt gttaattcca ttatcaacaa
      361 gtatgaaaac attgagtgat ttaacgaagc aagcttgcga taattttgtg tcacgttata
      421 cgagtgaggt tgatagcata tctttaaaag aatcagagct tgaagaagat atcagatcat
      481 taagtcaaca aattacgcga tatgaaaatt tgaataacaa tttgaaaaag catgcttccg
      541 ataatcagca agccatttca tcgaaccaac aaataatacg aacattaggt caacaaaaac
      601 atgaattaga agagaagcta cgcaaattgc gtgagtttaa tcaaaaatca ccagaaatat
      661 ttaaagaagt tgaagaattt caaaaaattg tccaacaagg acttacccaa gcgcagaatt
      721 tttggaactt ttcaacaaat caatttaata ttccttcagg taaagaactt gattgggcca
      781 aagcaagtca tgaaaaatat ttgaaagttg ctatggggaa aattgaacat aaagcagaga
      841 aagaaacttt aaataaagca gactttgctg ttataaaggc atatgccaaa gaacatccag
      901 aagacgatat cccgaaaagt atattgaaat atataaatga caataaagac agtattaaaa
      961 gagatatagg attagatatt acgtcaacac ttttagagca aggcggtata aatgcaagta
     1021 aattcggtgt atttatcaat acagcaggtg gagtgaaagg cccagcaggt ccaaattcat
     1081 ttgtggaagt caaacgtaca tcaggtaatg tgtttataga aaatggtagt aaatttgcaa
     1141 aaggcggaaa atacctaggt aaaggtgttg ctggtgtagg atttggtata ggtatgtatg
     1201 atgaccttgc aaatgatgat aaaacatttg gagaggcgtt gtcgcataat ggtatgacgc
     1261 ttgcagctgg atctgcaggg acagcagttg gagctggatt agcgactttt gttttaggaa
     1321 gtaatccagt aggatgggtg attttagcag gtttggctat gagtacagta tttgcattag
     1381 gaacagattt aatttatcaa aataatattt ttggattaaa agataaagta gactgggtag
     1441 ggcataaaat cgataatagt atagatgttg ttaaaaaaac tacagaaaaa tccatggata
     1501 gtgttgggaa tgctgttagt gaagctaaaa atattattag caatcatata aatccaatga
     1561 aatgggcgtg gtgagttttg ctttttaata ttaaggttat taataaaaat ccgagatata
     1621 aagtcgttca atataatgat gagtatttgt taattgattt agtaagcact tggcttgtat
     1681 acttttttcc ttttattaat tggttcattc ccaaaaggta tgcaaaactt agtgagaagg
     1741 aacttgaaaa tttaaatgtt gataaacaaa ataaaaataa tattttctgg ccagttactg
     1801 gtagttcgtt tttatttgta gttattttac ggaaatatgt acatacgttt gaagttcaat
     1861 tagataataa gatt
//
LOCUS       DAKTGS010000222         1862 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.230, whole genome shotgun sequence.
ACCESSION   DAKTGS010000222 DAKTGS010000000
VERSION     DAKTGS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.230"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..369
                     /locus_tag="O0286_002783"
     CDS             <1..369
                     /locus_tag="O0286_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0171094.1"
                     /translation="KYSNSPTKLFDSGFQKIHMKISEINKIEVESVIPILKDKNVVFT
                     GNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVSKQRKAREYV
                     GNGAKIQFLNEEDLINLIKE"
     gene            375..716
                     /locus_tag="O0286_002784"
     CDS             375..716
                     /locus_tag="O0286_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171095.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            920..1102
                     /locus_tag="O0286_002785"
     CDS             920..1102
                     /locus_tag="O0286_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171096.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            1180..>1862
                     /locus_tag="O0286_002786"
     CDS             1180..>1862
                     /locus_tag="O0286_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0171097.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIEN
                     KLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSD
                     IMLLINRIN"
BASE COUNT          803 a          207 c          272 g          580 t
ORIGIN      
        1 aaatattcta actctccaac taaacttttc gattcaggat ttcaaaaaat tcatatgaaa
       61 atatctgaga ttaataaaat agaagtggaa agtgtaatcc ctattttaaa agataaaaat
      121 gttgttttta caggtaattt tgacactgaa aaacaagatt taatgatatt aactagaaag
      181 aaaggagctt atatcagaag tgacgtaact gcaaaaacag atattttagt cgaaggtgtt
      241 caagatgata aatataaaga tgtgaacgga ctagtttcaa aacaacgaaa agctcgagaa
      301 tatgttggaa atggtgcaaa aattcaattt ttaaatgaag aagacttaat aaatttaata
      361 aaggaataat aacgatgatc aaaaaaattt ttacaaaaaa gcatgtattc ttagttatag
      421 aagatgaaaa ccataatcac agtgatgctg tttttggaaa aagtatatta ctttcaattt
      481 acgtcggtgt gaataaaaag actaattcta aatcagggaa atttatatac cttgacagat
      541 ctaaaagaat cgttagacaa tctgatatca ccaaaataga atcagctaac gaaaatgatg
      601 tagattttta taatttactg aagaaagaaa aggaaattgt ttattccaaa aatatagtag
      661 ataaatacaa tttagcgaac tatataattt actacgaagt tagtactaaa gaataaacca
      721 atccattatt tcataatact aaccttaaat ttacagaggt tttaattatg aaacatgaaa
      781 aaagcaatct taactttaag tcttatattt attacctact acctcacttt taaatatatg
      841 tggattaaag aattgaagta ttaattatgc ttatttaaaa aagacgtcta tttcagcagt
      901 gtttgaaagg aagtttataa tgaaaataac taattgcaaa ataaaaagag aaactgtaat
      961 atacgaagtt ttaactagtg gtaatcaacc attcacttat gagttaccta aagatttatc
     1021 gtcacataat gcgcgtaaat acttggaatt tatttcacaa aaaatagatg gcgataagtt
     1081 aaccaaagaa gattcattat gattttacta atcaaaaaac gtctacaagt gtagacgttg
     1141 aatggtggtg agagtgtgag cgagaataaa ggagaaatga tgacgcataa tatagaaaaa
     1201 cgcattaata aattaaaaac ttctggaaat ccaaaattta aaaaattaga ttcagatatt
     1261 cactatttac tcaagagatt tgaaggtgaa aaaaaccata aaggttttta tccaaagttt
     1321 aaacaaggag aaatagtttt tgtagatttc ggtataaacg ttaataaaga attctctaat
     1381 tcacactttg caatagtgat gaataaaaat gattctaata cggaagatat agtaaatgtt
     1441 attcccttat cttctaaaga aaacaaaaag tatttaaaga tgaattttga tttgaaatgg
     1501 gagtattatt taagattgtt tttaaattta attagcgcgc aaaataattc agctatatta
     1561 aaagaagttt tcgataaaaa ataccaaaaa aacaacacag aattcatcac taaagattat
     1621 tttagtgaat ttatatctga tagtttagaa attgaaaata aattaaataa aattgacaga
     1681 aacattaata acatagtatc agcaattgat aaggtaaaaa aattaaaagg taatagttac
     1741 gcttgcataa attctttcca gccgattagt aagtttcgca taagaaaagt tttaccccaa
     1801 aaaattaaaa atccagtaat agattcttcg gatattatgt tactgataaa tagaattaat
     1861 aa
//
LOCUS       DAKTGS010000223         1771 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.231, whole genome shotgun sequence.
ACCESSION   DAKTGS010000223 DAKTGS010000000
VERSION     DAKTGS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.231"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            61..1771
                     /gene="sasG"
                     /locus_tag="O0286_002787"
                     /pseudo
     CDS             61..1771
                     /gene="sasG"
                     /locus_tag="O0286_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
BASE COUNT          731 a          246 c          364 g          430 t
ORIGIN      
        1 taaaattaca tgtaaattca ctgtaagcaa agtggaaaat atggaaaaag gagtatgcaa
       61 atgagagata agaaaggacc ggtaaataaa agagtagatt ttctatcaaa taaattgaat
      121 aaatattcaa taagaaaatt tacagttgga acagcatcta ttttaattgg ctcactaatg
      181 tatttgggaa ctcaacaaga ggcagaagca gctgaaaaca atattgagaa tccaactaca
      241 ttaaaagata atgtccaatc aaaagaagtg aagattgaag aagtaacaaa caaagacact
      301 gcaccacagg gtgtagaagc taaatctgaa gtaacttcaa acaaagacac aatcgaacat
      361 gaaccatcag taaaagctga agatatatca aaaaaggagg atacaccaaa agaagtagct
      421 gatgttgctg aagttcagcc gaaatcgtca gtcactcata acgcagagac acctaaggtt
      481 agaaaagctc gttctgttga tgaaggctct tttgatatta caagagattc taaaaatgta
      541 gttgaatcta ccccaattac aattcaaggt aaagaacatt ttgaaggtta cggaagtgtt
      601 gatatacaaa aaaaaccaac agatttaggg gtatcagagg taaccaggtt taatgttggt
      661 aatgaaagta atggtttgat aggagcttta caattaaaaa ataaaataga ttttagtaag
      721 gatttcaatt ttaaagttag agtggcaaat aaccatcaat caaataccac aggtgctgat
      781 ggttgggggt tcttatttag taaaggaaat gcagaagaat atttaactaa tggtggaatc
      841 cttggggata aaggtctggt aaattcaggc ggatttaaaa ttgatactgg atacatttat
      901 acaagttcca tggacaaaac tgaaaagcaa gctggacaag gttatagagg atacggagct
      961 tttgtgaaaa atgacagttc tggtaattca caaatggttg gagaaaatat tgataaatca
     1021 aaaactaatt ttttaaacta tgcggacaat tcaactaata catcagatgg aaagtttcat
     1081 gggcaacgtt taaatgatgt catcttaact tatgttgctt caactggtaa aatgagagca
     1141 gaatatgctg gtaaaacttg ggagacttca ataacagatt taggtttatc taaaaatcag
     1201 gcatataatt tcttaattac atctagtcaa agatggggcc ttaatcaagg gataaatgca
     1261 aatggctgga tgagaactga cttgaaaggt tcagagttta cttttacacc agaagcgcca
     1321 aaaacaataa cagaattaga aaaaaagttg aagagattcc attcaagaaa gaacgtaaat
     1381 ttaatccgga tttagcacca gggacagaaa aagtaacaag agaaggacaa aaaggtgaga
     1441 agacaataac gacaccaaca ctaaaaaatc cattaactgg agtaattatt agtaaaggtg
     1501 aaccaaaaga agagattaca aaagatccga ttaatgaatt aacagaatac ggacctgaaa
     1561 caatagcgcc aggtcatcga gacgaatttg atccgaagtt accaacagga gagaaagagg
     1621 aagttccagg taaaccagga attaagaatc cagaaacagg agacgtagtt agaccgccgg
     1681 tcgatagcgt aacaaaatat ggacctgtaa aaggagactc gattgtagaa aaagaagaga
     1741 ttccattcga gaaagaacgt aaatttaatc c
//
LOCUS       DAKTGS010000224         1743 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.232, whole genome shotgun sequence.
ACCESSION   DAKTGS010000224 DAKTGS010000000
VERSION     DAKTGS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.232"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            123..800
                     /gene="recU"
                     /locus_tag="O0286_002788"
     CDS             123..800
                     /gene="recU"
                     /locus_tag="O0286_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCY0171098.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
     gene            797..>1743
                     /locus_tag="O0286_002789"
     CDS             797..>1743
                     /locus_tag="O0286_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0171099.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKA"
BASE COUNT          647 a          258 c          300 g          538 t
ORIGIN      
        1 tccaacatta acggtaaatg tattataata ttattgtgtt aatgctgaaa tcacgttaaa
       61 ataagctttg gtgtattgtg tctttcgcat ttatactaat ttaagtttgg tgtttcacta
      121 taatgttgtg tataataaac aacgtttact accaaacttg tgtggtgaaa tttatgaatt
      181 atccaaatgg taaaccatat cgtaaaaata gtgctataga cggagggaaa aagaccgctg
      241 cctttagtaa tattgagtat ggtggacgtg gtatgtcact tgaaaaagat atcgaacatt
      301 caaatacgtt ttatcttaaa agcgacattg cagttattca caaaaagcct acgccagtac
      361 aaatagttaa tgtcaactat cctaagcgga gtaaagctgt gattaacgaa gcttattttc
      421 gtacaccttc aacaactgat tacaacggcg tttatcaagg ttattatatt gattttgaag
      481 caaaggaaac taaaaacaag acgtcctttc ctttaaataa tattcatgac catcaagtcg
      541 aacatatgaa aaatgcatat caacaaaaag gtattgtgtt tttaatgatt cgttttaaaa
      601 cgctagatga agtttatctt ttaccctatt caaaattcga agtattttgg aagagatata
      661 aagataatat taaaaagtct ataacagttg atgaaatacg aaaaaatggt taccatattc
      721 cttatcagta tcaaccaaga ttagactatc taaaagcagt tgataagttg atattagatg
      781 aaagtgagga ccgcgtatga cggaaaacaa aggatcttct cagcctaaga aaaacggtaa
      841 taatggtggg aaatccaact caaaaaagaa tagaaatgtg aagagaacga ttattaagat
      901 tattggcttc atgattattg catttttcgt tgttctttta ctaggtatct tattgtttgc
      961 ttattatgct tggaaagcac ctgcttttac cgaagctaaa ttacaagatc cgattcctgc
     1021 aaagatatat gacaagaacg gagaacttgt taaaacatta gataatggcc aaagacatga
     1081 gcatgtaaat ttaaaagacg tgccgaaatc aatgaaagac gcagtacttg caactgaaga
     1141 caatcgtttc tacgaacatg gcgcacttga ttataaacgt ttattcggtg caattggtaa
     1201 gaacttgact ggtggatttg gttctgaagg tgcctcaaca ttaacacaac aagttgttaa
     1261 agatgcattt ttatcacaac ataaatctat tggacgtaaa gctcaagaag catacttatc
     1321 atatcgttta gaacaagagt atagtaaaga tgatatcttc caagtatatc taaacaaaat
     1381 ttactattct gatggcgtaa caggtattaa agctgctgct aagtattact ttaataaaga
     1441 tttaaaagat ttaaacttag cggaagaagc ttatttagcc ggtttacctc aggttccaaa
     1501 caactataat atttatgatc atccaaaagc tgctgaagat cgtaaaaaca ctgttttata
     1561 cttaatgcat tatcataaac gcattacaga taaacagtgg gaagatgcta agaaaatcga
     1621 tttaaaagcg aacttagtaa atcgtactcc tgaagaacgt caaaacattg atacaaatca
     1681 agattctgag tataattcat acgttaactt tgtaaaatct gaattaatga ataataaagc
     1741 att
//
LOCUS       DAKTGS010000225         1681 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.233, whole genome shotgun sequence.
ACCESSION   DAKTGS010000225 DAKTGS010000000
VERSION     DAKTGS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.233"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..263)
                     /locus_tag="O0286_002790"
     CDS             complement(<1..263)
                     /locus_tag="O0286_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0171100.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYS"
     gene            complement(387..1016)
                     /locus_tag="O0286_002791"
     CDS             complement(387..1016)
                     /locus_tag="O0286_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0171101.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            complement(1469..>1681)
                     /locus_tag="O0286_002792"
     CDS             complement(1469..>1681)
                     /locus_tag="O0286_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0171102.1"
                     /translation="IPTFKQGLLIVFAYFFLGSASIFTLTILLTTESQNIIIFLTWVI
                     ITMFFFLVNMASIGNKNVHVILRINE"
BASE COUNT          605 a          289 c          185 g          602 t
ORIGIN      
        1 gaataccttc ttaacataac accaaataag accgtacttc ctgccactgg ccaaaaagaa
       61 ttgattttag ctgtcttcac aatgtttaaa gtttcaaaat ctttttcgct gattttgacg
      121 tacttttttg gaatcagcca attaatcatt gggaaaaaga gtgctaacca tgtacttact
      181 aaatcaatca tcaaatattc atctttgtat ttgataattc tatatttggg attcttatta
      241 atattttcag tttcacaaag caatgtctcc acttcttttc aattggtttt atattgatac
      301 actttcatat acattagaag tcatttaaat tctactagcg tcttaaaagg attgtgtcga
      361 caacatttag aatattttaa atgtaatcaa ttatttttca ttataacgcg aactttttca
      421 ttacctatcg aagtcatatt taacaaaaag aaaagcattg tcatcataat ccaataaata
      481 aatattatta tattttgagg ttcgattgtc ataagggcaa ttaatgaaaa tattgaaaaa
      541 cttccagcga aaatatatcc gaaaactaaa aaacagccaa gtttaaacgt tggtattaat
      601 ataactctct tatttttttc aatatttgta tcaaacactt ttaacttaag ctttctattt
      661 aagttaaaat aaaaaactat aacacaaatt aaagcgagag cacacaatat cattacaact
      721 agcttatcta attgaacatc aaaaacatgt atgtatttcc ttaaagtaac tccaaacaaa
      781 accgaacttc ctataactga ccagaaaaca ttatttttat ttggtttaac aacatttaaa
      841 ttttcaaatt cttcttcgct aattatcgcg tacttttttg gaatgaacca attaatgaaa
      901 ggaaaaaagt acacgaacca agtgcttact aaatcgacta ataagtattc accgttatgt
      961 tgaatgactt tgtatttcgg atttttataa atgactctga catcgcaaag caacatttac
     1021 cactcccgtt tcattagttt taaatgatcc ccaatacttt ttgtaccttc accaactatc
     1081 gttccaacac tatctacagc tttttctgtg gtatttttaa acagcgtcta tttaatgtct
     1141 tatccaatta gttttatctc ttaatccaaa tttattagta tgaaaagtcc aatttttaag
     1201 aattgtctaa acagattccg caacaaaacc agctaaaacc gtccatacta ttggattaaa
     1261 aacgccaatg ttataagagc tatcatacaa aataacacgc tctatatttc atttttaaat
     1321 taaaacagtt ttttccttta tcaatcacaa tgggcatttt cacttggtat attcaaacat
     1381 tactatctat tttcctgatt taagatatga ttatattttt ttgaaccacc gtttaaatat
     1441 gcgatactac caatttcgat catttttact actcgtttat tcttaaaata acatgaacat
     1501 ttttattacc tatcgaagcc atattcacta aaaagaaaaa catcgtgata ataacccaag
     1561 ttaaaaatat tattatattt tgtgactctg tcgtcaaaag aatggttaaa gtaaatattg
     1621 aagcgcttcc taaaaaaaag tacgcaaaaa ctattaacaa tccttgtttg aaagtcggta
     1681 t
//
LOCUS       DAKTGS010000226         1675 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.234, whole genome shotgun sequence.
ACCESSION   DAKTGS010000226 DAKTGS010000000
VERSION     DAKTGS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.234"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            227..382
                     /locus_tag="O0286_002793"
     CDS             227..382
                     /locus_tag="O0286_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171103.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            390..911
                     /locus_tag="O0286_002794"
                     /pseudo
     CDS             390..911
                     /locus_tag="O0286_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1050..1580
                     /locus_tag="O0286_002795"
     CDS             1050..1580
                     /locus_tag="O0286_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0171104.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
BASE COUNT          631 a          187 c          303 g          554 t
ORIGIN      
        1 tttttaaaaa aacttgtaat ataccgaaag atttaaatgt aaaattttga tttgttaaga
       61 aattacgttt gtaaaaataa aaaaatcaac ttatttgtat gagataaata tgtattgaag
      121 aagacgtgtt attaatttgg aaaatactgg tcaaagatgg gagctcttaa aagcgttatt
      181 gtatttttta gtcaatacaa atagattgcc gtaataataa tcgtacttga tggttaaaaa
      241 attacttaag gctataaagc aaaacttttt atatgagcag tcgaatataa cgtttaaaat
      301 gattgttttt ggatataaac gattaagtaa aatgcttttt cagtttgaaa ttaatcatat
      361 aaatttctta tgggagggtt gatatcttaa tgattaacat tatttcagct ataggatcta
      421 ttggaacatt tattatggct ttattttatt ttgtatcagt ttcagttcaa ctttatcaaa
      481 tgaaaattag ctttctgcca gctttaggtt ttaaccaaat tttattagaa agggaggagg
      541 atcaacttaa tataatgaat tcggcaacag aagagcatca tcataaagat tatattaaac
      601 tatataattt aggtggcggt gctgctaaaa aaattgcaat agaggtttta ttggggaagg
      661 ataaagtcat tcagaaaaaa tacgtgcata ttttacctag taaagaaggg tacatgttac
      721 caattaataa aaatgtgtac gaagaattag aaagaacgat tgagaacaat ggtcatgaag
      781 ctgatttgaa tgtacgtatg acttattatc ataatgtaag tcgcaaacaa taggaagtta
      841 tattaaaagg tcaaatcgac cgttttaata cttataataa taaagaaatt tatgatttgc
      901 agtttatcta aaaattgatt taagagggta gttgtttatt gcgaaaaata tcattcaatt
      961 ttaatgaaat aatggcgtca ttactataaa atattacttt atgttgtaat gcatttttct
     1021 ataagataga actaaaagga ggggcaaaga tgcaaattag acaaatacat caacatgact
     1081 ttgctcaagt ggaccagtta attagaacgg catttgaaaa tagtgaacat ggttatggta
     1141 atgaatcaga gctagtagac caaattcgtc taagtgatac gtatgacaat accttagaat
     1201 tagtagctgt tcttcaaaat gaagttgtag ggcacggttt actaagtgaa gtttatcttg
     1261 ataacgaggc acaacgggaa attggattag tgttagcacc tgtatctgtt gatattcatc
     1321 atcaaaataa aggtattggg aagcgattga ttcaagcatt agaacgagaa gcaatattaa
     1381 aaggatataa ttttatcagt gtattaggat ggccgacgta ttatgccaat ctaggatatc
     1441 aacgcgcaag tatgtacgac atttatccac catatgatgg tataccagac gaagcgtttt
     1501 taattaaaga attaaaagtg aacagtttag cgggaaaaac aggtaccata aattacacat
     1561 ctgcttttga aaaaatatga tttcaagcta ggattacatt aggtagagtt catattaata
     1621 ataaaaaatg tttgcaatca aatcgtacgt tgtcgtttgt aattcttaaa atagc
//
LOCUS       DAKTGS010000227         1581 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.235, whole genome shotgun sequence.
ACCESSION   DAKTGS010000227 DAKTGS010000000
VERSION     DAKTGS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.235"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            86..586
                     /locus_tag="O0286_002796"
                     /pseudo
     CDS             86..586
                     /locus_tag="O0286_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(646..1143)
                     /locus_tag="O0286_002797"
     CDS             complement(646..1143)
                     /locus_tag="O0286_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0171105.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDH
                     CASPY"
     gene            complement(1145..1231)
                     /locus_tag="O0286_002798"
                     /pseudo
     CDS             complement(1145..1231)
                     /locus_tag="O0286_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1246..1572
                     /locus_tag="O0286_002799"
                     /pseudo
     CDS             1246..1572
                     /locus_tag="O0286_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          534 a          247 c          241 g          559 t
ORIGIN      
        1 cctattattt aaaacgtcgt gaagaaaaag gtaaataaat aagccttatg tgctaaactc
       61 aatatgtatt tcctaccaaa gaaaacataa ggagtttgga catcttgaag gagatactat
      121 tgttggtcgt cgtcatggta gtgcaatcat tactttggta gaaagagtat ccaagatgat
      181 tatcacctta cgacccttag gtcgtaaggc aactgatata gaaaacgctc tacactcaat
      241 gttttcatct ttaccttctc atatatttaa atcaattact tttgattgtg gtaaggagtt
      301 ctcaaactgg aaaacattat gtaactgtca cgatatatct atattttttg ctgatccagg
      361 cacgccatca cagcgaggtt taaatgaaca ttctaacgga atacttagac gcagtggatt
      421 agataaagaa ctagatttca atttagtaac agacgatcat atcatcagtg ttgctcaaaa
      481 aatcaatcat cgtcctagaa aatctttagg ttatagaaca ccattggaag tatttatgag
      541 tttcatcgaa gatgataaac ttgtccagct taatttgaca atctaaagat ttataaaaaa
      601 gccgttggtc tcttcgaaac tacggctttt ctctgtgcta taaaatcagt atggcgaagc
      661 acagtgatca ttgaattctg actttatata atcaagagta tctttggata cactgatgat
      721 atctataaca gcattgctaa tgtagaatat attttcttga ttatagaata caataaatgg
      781 ctccactaaa ccttttttaa tatataaaga ataatcaatt aagtctttgt tttttatatt
      841 tttaatagtt gcaataacat acttctcatc ggtagaatgg atttcttcgt accagttagt
      901 aatattaaaa aaacgaccaa ttcttgtttt ttctctaaat ttaacaggat ataagtattc
      961 agacaaaaat gtacatttcg tctttgattg gttattaaat ataacattaa taatattaga
     1021 aatttgcttt ttattgagaa catcaatatc taagcgtatc ctatttttct caggtgcttg
     1081 aaaataggga atttcatgtt ctaaatcaaa atgtatttgt tgtgtttcaa ggttaataat
     1141 cattttaatc tccaattctt tttaggttag catcgtatgt accacttctt gttttcttgc
     1201 tacctaaatt cttaatactg cttgcacctt ttcattttat cttctcatat atttaaatca
     1261 attacttttg attgtggtaa ggagttctca aactggaaaa caatatgtaa ctgtcacgat
     1321 atatctatat tttttgctga tccaggcacg ccatcacagc gaggtttaaa tgaacattct
     1381 aacggaataa ttagacgcag tggattagat aaagaactag atttcaattt agtaacggat
     1441 gatcatatca tcagtgttgc tcaaaaaatc aatcatcatc ctagaaaatc tttaggttat
     1501 agaacaccat tggaagtatt tatgagtttc atcgaagatg ataaacttgt ccagcttaat
     1561 ttgacaatct aaagatttat a
//
LOCUS       DAKTGS010000228         1574 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.236, whole genome shotgun sequence.
ACCESSION   DAKTGS010000228 DAKTGS010000000
VERSION     DAKTGS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.236"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            63..617
                     /locus_tag="O0286_002800"
     CDS             63..617
                     /locus_tag="O0286_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0171106.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
     gene            complement(852..>1574)
                     /locus_tag="O0286_002801"
     CDS             complement(852..>1574)
                     /locus_tag="O0286_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0171107.1"
                     /translation="LLEYLLVFETHIKSRISYYFSEKYREPHSYLYFKNYSSDTSKTD
                     SIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLWILVNYLTLGNVSKMYSNLDDDLRL
                     EVAKDYKRKLERDYKTRVQITPSDVDSILQQAHMFRNVCAHEERLYDYKIDRAKSRAN
                     IFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLNKHDYIKLMKNMDKLISNYSHSFYT
                     ITIDDLYTKMNFPDQTKILDML"
BASE COUNT          593 a          222 c          215 g          544 t
ORIGIN      
        1 ataaataata attgtaaaat tactttgtaa tcgattgcaa ataaattata ggagaaaata
       61 aaatgaataa aaaactatta acaaaaacat tgatagcaag tgctttagtt ttaacaacag
      121 taggttcagg ttttcattct tcttcaaatt ataatggtat taataacgtt gcaaaagctg
      181 agcaaacgac agataacgaa ttgtggaaaa atgtaagaga cgctttaaaa gaagcgaata
      241 ttatcgataa aacagataat gaaaatgtca aagtgactta taaattagca aatggcggtg
      301 aaaacagtat tgaaggtacc gccaatttaa atgaacttag caattctaat aacaaaccga
      361 tcaatagcga tagtgtaaaa agcattgatt tgtcaaggaa aaatcccaat ggagatagat
      421 ttgacgcaaa tgatgcatgg aaaaaattaa ctgataaatt aaaagaaaag cagattgtga
      481 aagacggtga tacagtaact attcatagta aagacgaaaa agaccctaaa atctcagcta
      541 aagtcggtga agtctataat gataacaaac gattgatgtt gtttaaaaag gatatagata
      601 aaatcactat aaactagtta tagataaaga caggttactt ttttaatgta acttgtcttt
      661 ttttaagagg cagtatctca aatattaatt tataatttct aaatctatat ctatacttta
      721 tctatttcta aatctatatc tatactttat ctatttctat atacacactc atccctcact
      781 gcaacacagg gcgtttctca gcgtcttaaa ataaaaaaac gccactcgta agtgacatta
      841 aaaaatatct tttataacat atccagtatt tttgtttgat ctggaaaatt catttttgta
      901 tataggtcat ctatagtaat tgtatagaaa gaatgtgaat aattacttat tagtttatcc
      961 atatttttca tcaattttat gtaatcatgt ttattcaaaa atagacatag tgaaatcaac
     1021 aaatcgaata cataactacc attcattgta ggaacgtatt ctttatcgta tattttgtta
     1081 taattggcga atatattagc tctactttta gccctgtcta ttttataatc atacaatctt
     1141 tcttcatgcg cacacacgtt acgaaacata tgtgcttgtt gtagtatact gtctacatct
     1201 gatggagtta tttgaacacg tgttttataa tctctttcca atttcctttt atagtcttta
     1261 gcaacttcca atcgaagatc atcatccaaa ttggaataca tttttgaaac attacctaaa
     1321 gttaaataat tcaccaatat ccatagtggc actccattat gagtattaat ataatgtttt
     1381 aatggtttat tttttctatt actcataacc gagctaaatg tagcaaccat tttcacgatg
     1441 ctatctgtct tacttgtgtc agatgaataa tttttaaagt ataaatatga atgtggttct
     1501 ctatattttt cgctaaaata atatgaaatt cttgatttaa tatgagtttc aaatactaac
     1561 aaatactcta ataa
//
LOCUS       DAKTGS010000229         1574 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.237, whole genome shotgun sequence.
ACCESSION   DAKTGS010000229 DAKTGS010000000
VERSION     DAKTGS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.237"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            117..905
                     /locus_tag="O0286_002802"
     CDS             117..905
                     /locus_tag="O0286_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0171108.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(1156..1530)
                     /locus_tag="O0286_002803"
     CDS             complement(1156..1530)
                     /locus_tag="O0286_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0171109.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          551 a          206 c          254 g          563 t
ORIGIN      
        1 ttttattgtt ggaataactt ttggtaattt ataaataatt taaaaaaatt gtttataaaa
       61 tggaagcgta tatagaatga aggttgggta tatagtttat tgagggaggt gtcacaatga
      121 ataaagtcac aattaatcct caaatccaat taacttatca aattgaaggt aaaggggatc
      181 ctataatatt acttcatgga ttggatggta atttagctgg atttgaagat ttgcaacatc
      241 aactagcatc atcatataaa gtacttactt acgatttaag aggtcatggc aagtcttcta
      301 aaagtgaatc atacgattta aacgatcacg ttgaggattt aaaaattcta atggagaagt
      361 taaatattca tgaggcacat attctaggac atgatttagg tggggtagtt gctaagttat
      421 ttacagataa atatgcttat cgtgtaaaat cattaactac cattgcatcg aagaaagatg
      481 acttaataca cagctttact caattgttaa tacaatatca agatgatata gcgggtttta
      541 ataagtctga agcgtatatt cttttatttt ctaaattgtt tagaaatcaa gagaagacga
      601 tgaaatggta tcaaaaacaa agaatatata gcattaagtc tgaggatgat agtgcggtgg
      661 caattcgttc attaattttg cataaagatg aacctatgta tttaaaaaaa cgtacatgtg
      721 tacctacttt gttaattaat ggggaacatg atcctttgat taaagataag aatcatttta
      781 aattggaagc gcatttttta aatgttacga aaaaaatctt cgaacattca ggacatgcac
      841 cgcatattga agaaccagaa gcatttatga attattattt aaaattttta aaaagcgtat
      901 cataatatgt gatatataaa cctagggcat aaagtcctta ggcaatgtga aaaagctgat
      961 tactattcat tatttgatag aaatcagctt tttttgaaat gtatttgata tatactgctc
     1021 gttatgcggc tatcttcctt atattaagtg ccattagtgc aaaacctctt aacaattagg
     1081 taaaaagagc ataaaaaaag gaagtttaat agaatgtatc atctatcaaa cttcaccaaa
     1141 ttgcgctaaa caaaattata gttcaatttc gttgtttgct tcagtgattc gtttatttac
     1201 tcgactcaat aatgattcga tttttttacg ttgttgtgca ttaacaagaa ttaatacagt
     1261 tctttcatca tgctcattac gttttttatc gaagtaatct tcttgagata aaattttaac
     1321 tgctttaaca acttgtggtt gtttgtagtt taaatgatta ataatatctt taagatagta
     1381 ttctttctct ttgttttcgc tgatgtatgt caatacagcg aattcttcaa agctaattga
     1441 aaattccttt ttaattaaac tttttaattt gtcagcataa gtgaccattg ataacaactc
     1501 aaagcaatca ttgatttttg taattgccat gtttaaaacc tccctatttg atgcatcttg
     1561 ctcgatacat ttgc
//
LOCUS       DAKTGS010000230         1571 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.238, whole genome shotgun sequence.
ACCESSION   DAKTGS010000230 DAKTGS010000000
VERSION     DAKTGS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.238"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            210..1553
                     /gene="brnQ"
                     /locus_tag="O0286_002804"
     CDS             210..1553
                     /gene="brnQ"
                     /locus_tag="O0286_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0171110.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT          497 a          231 c          255 g          588 t
ORIGIN      
        1 gagtttatat gaaattgtga ataaaagtca tcagttacta tttaaaaagt ggatttgaag
       61 aattttattt tctgacataa tttgatttaa acaacacatg tgttttgttt ctgctatgta
      121 caacatattt catatagact ctttattatt ttttgtgcta aaatatttac ttgtaaatta
      181 tttcgtgttt taaaaaaagg agtaactcta tgaataaaaa tacatgggtc attgggttta
      241 cgctcttcgc tatgtttttt ggcgcaggca atctaatttt tcctcctaat ctcggattgg
      301 atagtggtca atttttctgg cccgccattt tagcgtttgt tctaactggg attggtttac
      361 cattattagg tgtgattgta ggtgcacttg ataaagaagg atatattggc gcattaaata
      421 aaatttcacc taaattttca atattgttct taatcatcat ttatttgact ataggaccac
      481 tttttgcaat acctagaact gcatctacat cttttgaaat gacaattaca ccaattatac
      541 atagcaatag tagtatcgct ttatttatat ttacgattat ctacttcata gtcgttttgt
      601 atatttgttt aaatccatct aagttaatcg atcgtattgg ttcattatta acaccattat
      661 tattgattac tattttagcg atgattatta aaggatactt agactttagc ggtaatagtg
      721 ctggaaaggg caatgaagca ctatatcatt ctaatttttc aagttttgct gaaggcttta
      781 cacaaggcta tttaacaatg gatgccattg cagcaattgc tttttcaatg attgttgtta
      841 atgcagtaaa actaacaggc attactaaaa caaatcaaat attcaaacaa actttgactg
      901 ctggtttaat tgcagccgta gctttaattt tcatatatat ttcattaggt tatattggta
      961 atcatatgcc agtaagtgac atgacgttag atcaattgaa atccaaagat cgaaacattg
     1021 ggacatattt attaacgaca atggcttcaa caggatttgg ttcattcgga aaatatttat
     1081 tgggcatcat tgtggcgctg gcatgtctaa ctacagcatg cgggcttatt gttgcagttt
     1141 ctgaatattt ccatagaatc gtacctaaag tatcatacaa agcatttgta ttagttttca
     1201 ttttaatgag ttttattatt gctaaccaag gtttaaatgc tgttatctca atgtcaattc
     1261 cggtattaag cattgtatac ccagtagcaa taactgttgt attattaatt ttaattgcca
     1321 aattcatacc gacaaaacgc atttcacaac aaattccagt tattatcgta tttatattgt
     1381 cgattttcag tgttattagt aagttaggtt ggctgaaaat taactttata gaatcattgc
     1441 ctctaagagc gtattcttta gagtggttcc cagtagcaat tattgcaacg atattaggct
     1501 atctagtcgg catatttgta aaacaagatc caattaaata tcaacaggaa taacgaataa
     1561 tataaaagag g
//
LOCUS       DAKTGS010000231         1562 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.239, whole genome shotgun sequence.
ACCESSION   DAKTGS010000231 DAKTGS010000000
VERSION     DAKTGS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.239"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            416..520
                     /locus_tag="O0286_002805"
     CDS             416..520
                     /locus_tag="O0286_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171111.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            649..900
                     /locus_tag="O0286_002806"
     CDS             649..900
                     /locus_tag="O0286_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171112.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            976..1224
                     /locus_tag="O0286_002807"
                     /pseudo
     CDS             976..1224
                     /locus_tag="O0286_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          622 a          177 c          270 g          493 t
ORIGIN      
        1 aaatagtaga ttccaatttg tgaaaaagga gtatgtcgtt tcatttagaa tttattgcac
       61 gaaattagca tcgaataagg gaaaataagt gttaagtttt aaatgataaa aaagattgga
      121 atggatcgtc ttgaaatgct cccttcaaag ttttcatttt ttcaatgtcg acttcgaagg
      181 gggcattttc attaaattgt tatagctttt tatatttgta taatgaacat ataagtttaa
      241 gaaggtgcga gtgaaggaaa taaaaaagct caaatgtacc aaattgttaa tcttaataaa
      301 tctctacttt ataaagattg aatggacatt cgagcgttaa tcagtcagga gggactttcc
      361 ctcctacaat ttaataataa tacttgcttc accactatac aaggagtgag ttgttatgtt
      421 caaagtgaat tattcgattt taagttatta tccagaatat aatatcgcag taagttggca
      481 acgtttaaga gaaggaaaaa caataaaaaa caagatttaa tactgctgcg tcatgaggcg
      541 cttgaacatt atttgatgaa taagtataat ttcaactatg attatgcaca taaaattgta
      601 tcaaaaaaat acgattattc aatttttata aaaaagaagg tggattaaat gcttacatta
      661 ataaaattga aagaagatga acaggttata atatatgaat atatacctga agatgatata
      721 agtaacggta aaggttcagt aacttttaat aaaaaagatg cagaggttat agatttctca
      781 ttatctgaaa tagaaaatga agaatatttt atgttatatc gtaataagtc tttttctgta
      841 gtaagagact ttatcgagaa gcaagaattt cccgaaaatt ataaaatagc atggtattaa
      901 agcatccttt ctacacagat aaagagaaag cgatgctatt tttatatgct tttttcatta
      961 ttcgttgaaa aagaagagtc acaaaagtta acatatttaa aagaacttgg tgaagatggc
     1021 gaatataaat atgttgccaa aatagatagt aaaacatcta aattatgtca ttcactcaac
     1081 ggaaaaatat ttaaagttaa agatatgata ccaggtgtga atgcgccacc tatgcatcct
     1141 tggtgtagaa gtaccacagt gccacatgtc ggcaattggc gagacaagtt ctttaaagag
     1201 cgtgaaggta aatatcaagt agaaggttgt tttattgaat caggtgcact taataataaa
     1261 agtgatgaat acggtatcaa aaggaacaga catgctcaaa tttattataa ttcagttaga
     1321 aatcgagata aacagataga gatttctaaa attgctaaaa atactaatat aaataaaaat
     1381 ttaattcaaa gagtttatga acatatattt gaaaataagt atttattaga aagtggattt
     1441 aaacaatttg atcctgactt ttatatggct caaagttggc aacgtttaag agaagggaaa
     1501 aatattaaaa aaatggatat aataatgtag aaacatgaag ctcttgaaca ctatctaatg
     1561 aa
//
LOCUS       DAKTGS010000232         1562 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.240, whole genome shotgun sequence.
ACCESSION   DAKTGS010000232 DAKTGS010000000
VERSION     DAKTGS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.240"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(25..306)
                     /locus_tag="O0286_002808"
     CDS             complement(25..306)
                     /locus_tag="O0286_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0171113.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            595..1290
                     /locus_tag="O0286_002809"
     CDS             595..1290
                     /locus_tag="O0286_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0171114.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT          555 a          225 c          281 g          501 t
ORIGIN      
        1 ctgctatata taaacgaaat gatattagaa tccttcattt tcaacttgtt taaaattacg
       61 tttaatgccg ttataagaat atacaacatt atataatttt acttgggcgt catggacact
      121 tgagaagggc attcttacat cgtccatatt tttaacttcg tgtcgaaatg ctttttcagc
      181 tttttgtctt tctgcaagag acatacttag ccattcttct ttgctaaata aatcttcgaa
      241 tcgaaaatca aatgcggttc ttttactttc tgctcttaat gctaaaacac gctcatgata
      301 tgtcacgttt tattcctccc tctaatcatt caatcgaatg ataaatcaca gtattaactt
      361 tattactaat ataatgtttt tacaataaag aagtgacgat ttttcaacat ttttaaaaat
      421 aaaaaatata agcaacgatt aattgtatgt ttcgaatggc ttgtaatgta atttatctat
      481 gaaaatgaag cgagttatta tgaccgttat atgaattgtg atacgctttt taaaagtaat
      541 atgtatttat gatttaccct gaaaaaataa aaagaatcaa aggagtcgat cattatgaca
      601 gtattaacag ataaagtagc agtagttaca ggtgcaggta gtggtattgg agaagcaatt
      661 gcaacattac tacatgaaga aggggcaaaa gttgtcttag caggtagaaa taaagaaaaa
      721 ttacaaaacg tagcgaatca attgtcacaa gatagtgtga aggtagtgcc aacagatgta
      781 acgaataaag aagaagtcga tgaattgata aaaattgcac aacaaacatt cggtggtttg
      841 gatattgtta tcaatagtgc ggggcaaatg ttgtcgtcta agattactga ttatcaagta
      901 gatgagtggg atagtatgat tgatgtgaat atcaaaggca ctttatatac ggcacaggct
      961 gcattaccaa ctatgttaga acaatcaagt ggccatctta ttaacattgc atctatttct
     1021 ggctttgaag taacgaaaag tagtacgatt tatagtgcga cgaaagcagc agttcacact
     1081 attactcaag gattagaaaa agagttggca aagacaggcg ttaaagtaac aagcatttct
     1141 ccaggaatgg tagatacagc cataactgcc gcatacaatc caacagatcg taaaaaactt
     1201 gaaccacaag atattgcaga agcagtatta tatgcattaa cacaaccaaa gcacgttaac
     1261 gtgaatgaaa ttacagtgcg tcctgtataa aaaggtgttt gaggtctggg gtataggcat
     1321 ttaatgtaat agctatcgat tttgttaatg ttagtaaggc aataattaca tgagggcata
     1381 ataggcaatt attccttgta caattctcag taattgctga ttttaacaat caagttaatg
     1441 aaggcgacga tgttagtgtt atggtttata atctgtaatg ttcataatta tagattataa
     1501 aaattttttc attttccacc acattttagg agttacttta atgaacaaaa aacatgtttt
     1561 ta
//
LOCUS       DAKTGS010000233         1545 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.241, whole genome shotgun sequence.
ACCESSION   DAKTGS010000233 DAKTGS010000000
VERSION     DAKTGS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.241"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            135..746
                     /locus_tag="O0286_002810"
     CDS             135..746
                     /locus_tag="O0286_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0171115.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
     gene            882..1100
                     /locus_tag="O0286_002811"
     CDS             882..1100
                     /locus_tag="O0286_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171116.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            1232..>1545
                     /locus_tag="O0286_002812"
     CDS             1232..>1545
                     /locus_tag="O0286_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0171117.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSVYELYKQFQNLRNLFKEEGHEPWTSCEF
                     DFT"
BASE COUNT          564 a          151 c          281 g          549 t
ORIGIN      
        1 attctgtgaa gtatacttat atataagata atcaacttca acgtatatca ccatattatt
       61 ttatactcaa tgatgtgaat ggatttaagt ggattgatat taaagtaaaa gttatggaat
      121 attgaggtgt gaatatggag ttcttattat taattgtcgt agccggactg tattatatta
      181 tatatttaac tgctgtgatg tattctgaaa aaatagtagt attgcctata atcatctatg
      241 ccattgtgtt tgtaataatt ggtatcactt atatctttat aggcgacagc tatgatcaat
      301 taacaaattt caatgtgatt ttgtatatgg ggagtttgtt ttatgcatgg atggctatta
      361 gaaatctttg gaacagacca ctattactaa aatataagaa cattacagat agttcaagtg
      421 gaatagttaa taaatctgaa tacaattcag ttgaaagctt acgtataaat attgaaatag
      481 ctaagtataa agggattatt tctttgatag tagctatagt actaacggta ttaatgacat
      541 taaagtcaac acctcaaatc actgcagaaa cacgtgactt aagtatctca tttttcatac
      601 tcagcttatt tatcattatt atatttgcag tttgggattt gtttattaga gttagaaaag
      661 gagcgtttgc ttttgttgta ataaggccaa tattatttag ttgttggtta tttattttga
      721 atatgatttt atcaagatta ttataattgt attacatata ggtgtgggta aatttttatt
      781 tatgaaatga taaaaaagta tatactatta atatcttttt tactataatt ataaatagtt
      841 aaataaattg aaagacaaaa taatgagtag gagttaatga gatggaaaac caaaaacaag
      901 gcaatggctt aaaaattgca acatgggtat ttattgtatt aacagtagtt acaccgctat
      961 ttggtattgg aagtattgtt tgtagtatta attataaaaa atacgatgct gaaaaaggtt
     1021 cgaagttatt gcaaattgca attatcgtaa caataattgc ttttgtttta aatttattag
     1081 catatttagg tttaagataa gtaaaacgaa tttgaagaag cattaagcga catttgggtg
     1141 ttgtttaatg ctttttattt aacggaaata agtgtagaga ataaattaat agtttaatca
     1201 agagatattt cgaacacata ggggcgataa catgactttc gaagaaaaat taagtgaaat
     1261 gtatagcgag attgcgaata agattagcag catgataccg gtagagtggg aaaaggtata
     1321 tgcaatggca tatatagatg acggaggagg agaagtgttc tactattaca cagaacctgg
     1381 aagtaatgaa ttatactact atactagtgt attaaataaa tatgatatat cggaatcgga
     1441 atttatggat tcagtgtatg agttgtataa acaatttcaa aatttaagga atttatttaa
     1501 agaagaagga catgaaccat ggacatcatg cgaatttgac tttac
//
LOCUS       DAKTGS010000234         1526 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.242, whole genome shotgun sequence.
ACCESSION   DAKTGS010000234 DAKTGS010000000
VERSION     DAKTGS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.242"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            352..753
                     /locus_tag="O0286_002813"
     CDS             352..753
                     /locus_tag="O0286_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCY0171118.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            713..805
                     /locus_tag="O0286_002814"
     CDS             713..805
                     /locus_tag="O0286_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171119.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1122..1451)
                     /gene="ecb"
                     /locus_tag="O0286_002815"
     CDS             complement(1122..1451)
                     /gene="ecb"
                     /locus_tag="O0286_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCY0171120.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          554 a          225 c          202 g          545 t
ORIGIN      
        1 aaaaatatat atcaaaacag ctatatgtat aacaatttgg agtgtttgtc tatccaactt
       61 agcaaacaaa atgtagagaa atgtaaatat cgttttagca ataaaaatta ttcaatcata
      121 gttgagtgac gtttactact attattatac tatcaacaaa ttcaataata cctcaaataa
      181 aaataatacc atcaaattca tcattattct ttttcgattg cattaaacct tattaatttg
      241 cacttaattt taattaatga agtgttaact ggtaaatgta tgaaaccgtt tgtaaaatag
      301 cagtgtactc gcattagtaa gtacaaattt attttacagg gagaacttat tatgaaaaaa
      361 aatatcacaa aaactattat cgcatctaca gtaatcgctg caggtctttt aactcaaact
      421 aatgatgcta aagctttctt tagttatgaa tggaaaggtt tagaaatcgc aaaaaattta
      481 gcagatcaag ctaaaaagga cgatgaacgt attgataagt taatgaaaga gtctgataaa
      541 aatctaactc cttacaaagc tgaaactgtt aatgatctgt accttattgt taaaaaatta
      601 agccaaggtg atgtgaagaa agcagttgtc agaattaaag atggtggtcc tagagattac
      661 tatacttttg acttaactcg tcctttagaa gagaacagaa aaaatattaa agttgttaaa
      721 aacggtgaaa tcgactcgat tacttggtat taaaaaacat actgaattaa atagttgtac
      781 gccaaacgtt agaaaacaat gctaacgtat tggcgtgctt ttttatttta agtaacttcc
      841 aatttattta gcatctttac gactgtttaa taaagcacgt atgattaaca cggttgccac
      901 tatatccgtt acaattttta tgattgttaa tacattcgat ttccttttca caatagccac
      961 ctcgcttgtt caaaacataa acaactattg cattcacttt taagtaatta tttatattat
     1021 ttatcccaat aagctcacca ttcaaataaa cacaatatta taaaataatt atacctttgg
     1081 atatagcaaa aagccacact ctatagcatg gcttctatca attattttaa agtattatat
     1141 tttaaaacta gatcgatttg tctttgtaat tttttctttt cataactgtg ttggaaatga
     1201 attaaattaa cagctctttg tgctttacgg tgtgttgcaa cagttcttgt acgtttgaaa
     1261 aagtttacag ctttttgtgc atccacaact tttttattta cttgtttttt aaagtttgtt
     1321 tgatactgat catatttttt agcagcttca acgtttttag tttgtgcatg agattcaccg
     1381 gcaaacgttg ataccgttaa actaatagca gctatgctta aaattgattt cccaataaaa
     1441 ttctttttca taattaataa cacctatcct caaatattta ttttgtatat atttataata
     1501 atagacagat acaaaaaact acatta
//
LOCUS       DAKTGS010000235         1501 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.243, whole genome shotgun sequence.
ACCESSION   DAKTGS010000235 DAKTGS010000000
VERSION     DAKTGS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.243"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            171..374
                     /locus_tag="O0286_002816"
     CDS             171..374
                     /locus_tag="O0286_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0171121.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(371..607)
                     /locus_tag="O0286_002817"
     CDS             complement(371..607)
                     /locus_tag="O0286_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171122.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            832..1413
                     /locus_tag="O0286_002818"
     CDS             832..1413
                     /locus_tag="O0286_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0171123.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
BASE COUNT          547 a          222 c          234 g          498 t
ORIGIN      
        1 aatatttaca tactaaaaac tcttaaagtt gtataattcg ttaatatttt tagaatcaga
       61 tatcaaaaaa atgtgcaaaa tcacttttgc acatttacaa atactagttt tctgataaga
      121 ttaataataa cactttaaat agcgcttaat aaatgaaggg ggcaagtctt atgacgtttt
      181 acaatttcat catgggtttt caaaatgata acacaccatt tggtatattg gccgaacacg
      241 ttagtgaaga taaagcattc cctcgattag aagaaagaca ccaagtaatt agagcatatg
      301 tgatgtctaa ttacacagat catcaattaa ttgaaactac aaatagagct attagcttat
      361 atatggcaaa ttaatttgag tagtaccaat tatgatgtat tagtgcatcc caaatatctt
      421 ttgttttaaa gtttatttca tcatttctta tcgaaaatgg tgtaataatg tctttatcta
      481 accaagtgtt gataagttca tttggtacac catctaacaa catttcactt ttactaatta
      541 taaaacattc ccagtcaagt gaaacatttt gtggattcac ataattacat tgattatgat
      601 tatccataaa cactcactcc tttaaaattc tgtactcttc attgcgtttt accccgtcac
      661 attatctttt aaactaaaat catcattact tatgaaaaaa atgtacatca aaagcaaagg
      721 ttttcgctac cgaaaaagtt taaataatgg tttaatatat ttggtactca ttttaataaa
      781 aagagaatac attttgagct atcaatactt tttattgaag aggtgttatt gttggctaaa
      841 acgttatata taatgcgcca cggacaaact ttgtttaatt ttaagggact aattcaggga
      901 tttggagatt cgccgctaac agaacttgga attgctcaag ctcaaaaggc acgtagttat
      961 tatgaaacta aggggataaa cttcgattta tatgcatcat caacgcaaga acgcgcaagt
     1021 gacacacttg aaaatgttgc acctaaccaa tcgtatcaac gttttaaggg actgaaggaa
     1081 tggcattttg gaatatttga aggtgagtca gtctatctat ttgataatct atacaagcct
     1141 gaggacttat tcggagatcg aattgttcct ttcaaaggag aggcaaggca acaagttgaa
     1201 gaacgcattg tgaaaacttt acatgacatt atgtctcaaa caaagaataa tgcattagtc
     1261 gtgagtcatg gaacaataat gggagtattt ttaagatatt gccttaaact agatgaagca
     1321 ttaaagcata atatcggtaa ttgtaatatc ctgaaatttg aatatgacaa tggaacattt
     1381 aaatttgttg agttaattga tccaaattta taataaataa agcgttatac atagacacaa
     1441 gtatgtcttt tgtataacgc ttttaaaata caactacata acgctttaga ataataattg
     1501 a
//
LOCUS       DAKTGS010000236         1494 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.244, whole genome shotgun sequence.
ACCESSION   DAKTGS010000236 DAKTGS010000000
VERSION     DAKTGS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.244"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            276..1235
                     /gene="hyl"
                     /locus_tag="O0286_002819"
     CDS             276..1235
                     /gene="hyl"
                     /locus_tag="O0286_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCY0171124.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          566 a          219 c          230 g          479 t
ORIGIN      
        1 aaaattagct atgtcttttc cttgtttcat aaagaattta ttcaactttg actaaccctc
       61 gaaattgaaa tgcttccttt caaattttaa ataaaagtta aaaacatatt cttaaaataa
      121 tcactcatca tcactcagta atttatcagt tgctacatta aatatactaa tataaacgat
      181 tataaatatt tgatatgtct caactgcaat attctaaatt gacatattga ttattgtttc
      241 ctcaaatcgt tttaaaaaat agaaggatga tgaaaatgaa aacacgtata gtcagctcag
      301 taacaacaac actattgcta ggttccatat taatgaatcc tgtcgctaat gccgcagatt
      361 ctgatattaa tattaaaacc ggtactacag atattggaag caatactaca gtaaaaacag
      421 gtgatttagt cacttatgat aaagaaaatg gcatgcacaa aaaagtattt tatagtttta
      481 tcgatgataa aaatcataat aaaaaactgc tagttattag aacgaaaggt accattgctg
      541 gtcaatatag agtttatagc gaagaaggtg ctaacaaaag tggtttagcc tggccttcag
      601 cctttaaggt acagttgcaa ctacctgata atgaagtagc tcaaatatct gattactatc
      661 caagaaattc gattgataca aaagagtata tgagtacttt aacttatgga ttcaacggta
      721 atgttactgg tgatgataca ggaaaaattg gcggccttat tggtgcaaat gtttcgattg
      781 gtcatacact gaaatatgtt caacctgatt tcaaaacaat tttagagagc ccaactgata
      841 aaaaagtagg ctggaaagtg atatttaaca atatggtgaa tcaaaattgg ggaccatatg
      901 atagagattc ttggaacccg gtatatggca atcaactttt catgaaaact agaaatggct
      961 ctatgaaagc agcagataac ttccttgatc ctaacaaagc aagttctcta ttatcttcag
     1021 ggttttcacc agacttcgct acagttatta ctatggatag aaaagcatcc aaacaacaaa
     1081 caaatataga tgtaatatac gaacgagttc gtgatgacta ccaattgcac tggacttcaa
     1141 caaattggaa aggtaccaat actaaagata aatggataga tcgttcttca gaaagatata
     1201 aaatcgattg ggaaaaagaa gaaatgacaa attaatgtaa attatttgtt catgtacaaa
     1261 taaatataat ttataacttt agccgataac ttcagaaatg atgtttttcg gctaattttt
     1321 atactgaatt tagtttaatt aacttatgat atttttaatt taaactatct acttgatttg
     1381 ctttcctgac ttaaaaatta gactaaacat gacactatat gaggtaattt ttaaaataag
     1441 aaatttgaat tccatgttgt caaacccttt atataaaagt tttgaaattg ttca
//
LOCUS       DAKTGS010000237         1479 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.245, whole genome shotgun sequence.
ACCESSION   DAKTGS010000237 DAKTGS010000000
VERSION     DAKTGS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.245"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..>1479
                     /locus_tag="O0286_002820"
     CDS             <1..>1479
                     /locus_tag="O0286_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0171125.1"
                     /translation="TNNFKYTNISYLKELKGYIENDILDKMKSKYAKNIYLNFKKIFS
                     NLEQSVNNYLELEEEFEEKKKYLSEIYELIDEINIDPYIFLTEHNECNIYKISENEKL
                     ELQTYMSKIEKVLLKYQTYLKETCKECLFYPYLLVQGEAGIGKSHLLAHLSKKLRDEN
                     HIIYLFLGQFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDALNE
                     GEGKRLWGNYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEGFS
                     KEENYNPIVSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQTISVAEL
                     FTNVLKTVNINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEESYTVVNNI
                     AKEYVQKSNRFLEALIDENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEKYRNVDK
                     RDLVTKLQSDEKVTRYFQKEDDLSYNRGLINELFIKLANEFN"
BASE COUNT          615 a          145 c          217 g          502 t
ORIGIN      
        1 ttaccaataa ttttaaatat actaatattt catatctaaa agaattaaaa ggttatatcg
       61 aaaatgatat tttagataag atgaaatcaa aatacgcaaa gaatatttat ttgaatttca
      121 aaaaaatatt ctctaattta gaacaatcag tcaataatta tttggaactg gaagaagagt
      181 ttgaagaaaa gaaaaaatac ttaagtgaaa tttatgaatt gatagacgaa attaatattg
      241 acccttatat ttttctgaca gaacataatg aatgtaatat atacaaaata agtgagaatg
      301 aaaaattaga acttcaaact tatatgagta aaatcgaaaa agtattatta aaatatcaaa
      361 cgtatttaaa agaaacatgt aaagaatgct tgttttatcc ttatttgcta gttcaaggtg
      421 aagctggcat aggaaaatca caccttttag cgcatctatc taaaaaactt agagatgaaa
      481 atcatattat ttatctgttt ttgggacaat tttttactaa aaatgaagat ccatggcatc
      541 aaatacttaa tgatttagaa gttacaaatt ctgttgataa ttttttaagg tcaataagta
      601 ataaggccaa agaaacaaaa aaaagagctt ttattattat tgatgcgctt aatgaaggtg
      661 aaggtaaaag gttatgggga aattattttc aaagctttat caaccatata aagaagtatt
      721 ctaatatagc tttaatattt tctattagaa ccccttttga agatgttata ttacccaaaa
      781 acgcaataca agataacaat attgtagtat ttcagcatga aggttttagt aaggaagaaa
      841 actataatcc aattgtatct ttttgtgatt tttatggatt agagctacct aagttaccta
      901 tattaaatcc agaattcaac aatccattat ttttaaaact aatgtgtgag tactgtgtaa
      961 ataaatttaa ggaatttgac caaactataa gtgttgcaga gctatttaca aatgttctta
     1021 agacagtaaa tattaattta tctaaggaag ataaatttga ttttgataaa aatattaatg
     1081 ttgtacaaaa agtaataaaa ggattagttg aattaatgaa cgactcagaa tttaatcaac
     1141 taaattatga ggaatcctat actgttgtta ataatatagc taaagagtat gttcaaaagt
     1201 caaatagatt tttagaagcg ctaatagatg aaaatatatt aattaaaaat actggctata
     1261 aaggtgaaat gattatttat ttttcgtatg agcgtatggg agattacttt ttatcagaat
     1321 accttttaga gaaatataga aatgttgata aaagagattt agtaactaaa ttacagtcag
     1381 atgaaaaagt gacaaggtat tttcaaaagg aagacgattt atcttataat agaggtctta
     1441 ttaatgaact ttttattaaa ttagcaaatg aatttaata
//
LOCUS       DAKTGS010000238         1453 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.247, whole genome shotgun sequence.
ACCESSION   DAKTGS010000238 DAKTGS010000000
VERSION     DAKTGS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.247"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(323..754)
                     /locus_tag="O0286_002821"
     CDS             complement(323..754)
                     /locus_tag="O0286_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0171126.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDTILEKITSGDIN
                     NGEFDLVIMSNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
     gene            1256..>1453
                     /locus_tag="O0286_002822"
     CDS             1256..>1453
                     /locus_tag="O0286_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCY0171127.1"
                     /translation="MKKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISR
                     DLQELEIYKIPVENKKYIYKKI"
BASE COUNT          511 a          211 c          148 g          583 t
ORIGIN      
        1 gctacagtga taacatcctt gttaaattgt ttatatctga aatagttcat acagaagact
       61 cctttttgtt aaaattatac tatagattca actttgcaac agaacctata ttttgctata
      121 attattaaat taattgcctt tgtgttagta ttccctggat tactaacact ttggttagct
      181 gttctaagtg atacaggtgc agcaatatta gttatattaa attcattacg tttattaaaa
      241 aaataaaaaa gcctaaatac tctctcgatt tgtgagaaag tatttaggct ttttgaatta
      301 ataaatttct tacttcttat gtttatttaa caattagtac tgggacagaa cttcttttag
      361 caatcttatg tgtaacatct cctaaaacat gtttaatatt caaatcgact ctatgattac
      421 tcataattac taaatcaaat tcaccattat ttatatctcc agaagtaatt ttttctaaga
      481 ttgtatcttt aatatgccca aaatctacat ctattttata ttgaatatct cttttttcta
      541 attcacgtaa aaatggagtg agttttttct ctttttctct aataatatct tctttatgct
      601 tattataata cttgacacta attgcaagat cattttctgt tacaacgtga taaattacaa
      661 caactgaatc ttcatcggtg actttttcta aatagtcagg gatagcacta aaatcatttt
      721 cgaaatcata gggtaataat atatttttga acatctacac cagcctccta ttacttatta
      781 tttagatgtc tattacttat atatacccta aatatgtatg cttattctca tattcaaatt
      841 attcaaatat attccttatc atgtattaga ttctattttt tatattattt ttgtaattct
      901 ttgtataaac ctatttattt ataaagcgct ctaatacata cctaaatatc ttcaacttat
      961 aaactacttt ttatttcaat gcctttttcc aaacttcttt tatgagatat tgtggttatt
     1021 acctgacatt tacaatttac acatttcgct attattacgc aaattatttt gattttataa
     1081 ttatgatgac tataatctat tctaattaat ctctcctttt aggcataaaa taatctactt
     1141 ataattaaag catccactat ttcaaaatta tttgtaaatt gagatttata agtgattttt
     1201 atacagttat ttaaaatcta tttcataatc aaaaataata aagggggtaa taaaaatgaa
     1261 aaaagaaaaa cgtttaaatc ttatcttaac tgttatacaa cagaatcagt ttaataaaaa
     1321 acaacaaata gtggattaca tggcaagaca ttttggagtt tactatagct tgacaactat
     1381 ttctcgtgac ttacaagaat tagaaattta caaaatccct gttgaaaata aaaagtatat
     1441 ttacaagaaa ata
//
LOCUS       DAKTGS010000239         1415 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.248, whole genome shotgun sequence.
ACCESSION   DAKTGS010000239 DAKTGS010000000
VERSION     DAKTGS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.248"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(245..>1415)
                     /locus_tag="O0286_002823"
     CDS             complement(245..>1415)
                     /locus_tag="O0286_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0171128.1"
                     /translation="VQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFK
                     DVVNRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTK
                     SHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGG
                     SASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYML
                     AEMLKGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTP
                     QYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGS
                     IPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
BASE COUNT          422 a          264 c          254 g          475 t
ORIGIN      
        1 acaaccactt attcaaattc aataatttta catagctaaa gatgtgaata tgaaaaacta
       61 tttagatgtt tcaaaatgta tgtgaatgac tgattttacg gtttctacta atatagcaat
      121 agcgtatgaa tacttataaa aaaagcagga agttttacct tcccaccata aaagatgaag
      181 aacctaaaaa aatcctcggc tacaccataa atatggtata actgaggatt ttatgttgag
      241 tggattagtt gaatatacct gttaatccac cgctgtttct agattgttga gatctagtat
      301 tgttatttga ttgtgcagta ccactgctgt ttgctgatgt gtcactacca ccgtgtgtac
      361 tacgatttgt agtgttgtta tcttgactac cagaaacatt gattgatggg atactaccac
      421 ttactgagct aggacgttta aagtcttcgc catctctaga tgaaattttt gacatcacat
      481 tttcatataa gaactgtgga tattcttgtt ggctatgtcc cacaaatgag ttttcaccat
      541 attgtttaac tttactgaag cccatccaca ctgacatagt gtattgaggt gtaaagccgt
      601 taatccacac gtctttcgct gcattatcag gtaaattata ttgtgaataa gtttcagcac
      661 cgtaagtacc agtacctgtc ttagcaccca tatttactcc agatacacca tggccatatg
      721 cagaaccata tggtttaaat gtacctttta gcatctcagc taacatgtat gcagtgtaat
      781 cactcatcgc tttatggcta gtatgatcgt attcgattgt ttcaccatca cgagtaacta
      841 ctttttgaat tgaatgcgcg ttgttataag taccaccgtt agcgattgca gcaaatgctg
      901 atgctaattg tgttggtgag aattctgaag cagaaccacc aagtacttca gatggaccaa
      961 tatcgccttc gtagtttaag ccaagtttgg cagcgaattt cttaggtgca tcattaccag
     1021 cattttgctt aactgattgc caagctttta aagctgggat attgaaactt tgtcgtaaag
     1081 catcataaat agatacagta ccgtgactct tcgtatcata gtttctaaat gtagaaccat
     1141 caacttgata tgaagattca tcttgaatcg catggtttgt tgcccatttc atattttcaa
     1201 tggcaggtcc atacgctaag aaaggtttta aagatgaacc agtagggtga ggatctgttg
     1261 cttggtttct gttaacgacg tctttgaaat cacgtccacc agatatagca actaaaccac
     1321 cagttttact atcaagaatt gttgcaccaa cttgttggtc tttattcttg tagaagctac
     1381 cattatcaac atcattttgt aatgtttttt gaaca
//
LOCUS       DAKTGS010000240         1407 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.249, whole genome shotgun sequence.
ACCESSION   DAKTGS010000240 DAKTGS010000000
VERSION     DAKTGS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.249"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(31..249)
                     /locus_tag="O0286_002824"
     CDS             complement(31..249)
                     /locus_tag="O0286_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171129.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(311..595)
                     /locus_tag="O0286_002825"
     CDS             complement(311..595)
                     /locus_tag="O0286_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171130.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            complement(683..1252)
                     /locus_tag="O0286_002826"
     CDS             complement(683..1252)
                     /locus_tag="O0286_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171131.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
BASE COUNT          471 a          205 c          206 g          525 t
ORIGIN      
        1 cattaaaata attaaataaa aatattgcgt ttaatttaca gcgtcaaata tacttatttc
       61 taatgctttg gggtctactg aaacaagtaa agaatgatcg atgttactaa tattgccatt
      121 ctccaaattt atttctgtga gtatttggaa gctaccatta ggcaacggtt taacaataga
      181 caattgcttt tccgcttgtt gtattaaaaa aggttttgta gattgattat taatatgcca
      241 ttcactcatg tatgtttttc actcctgctt taaaataggg ttagaaagtt tatagttgag
      301 acattcatgt tcaaccaaaa ttttgttcga attcaataaa tgtcttgttt aaaatagaaa
      361 tattgtaaat gttatcgtcc aaaacttcac cagttaagta tttgttttga attaaaattt
      421 ggcagttagt taagaagtct tgataatcac gatcgcaaaa atagttttca cgtgcatctt
      481 tagcatcgcc aaaaaagtta gcgactgttt ctgtttctcc tttattcgaa cgttcaatat
      541 ataatttgta aaatttagct attgtatact tttgttcttt agttagttca ttcaaaatat
      601 tgggcctcct gaaatatcat ttgtaatcta tacccaattt attgcaaaac acaaactaat
      661 ttaactattt gatgaaactg tgttaataag ctttaacaag ccttagtttg tatggatcta
      721 taaaattatc tttaattgca tagggtgaaa taatatgtag tccataactt ttaactgatt
      781 tttcacttac accaaattta taagcttggt agataatttt agtacaatac gtaaattttt
      841 tgctgttcaa atttaatgta actagataac gatgatttgt attctcatag tttttcttaa
      901 cccattcagc cgctttttta cctgcaccag gatagctgca acgataaact ttcatccaat
      961 catttttgcc acttgcataa ttatatttaa aagattcgaa ggattgtgta gttggtttgt
     1021 cgccaggccc ctcaatttgt aaaatcgttt tatcatcaat cgcgatacta caatgaccaa
     1081 aaaatcccca catgacaggg ccttttgtaa caataatatc accaggttgt aattggaatt
     1141 tgtcatcttg aatttctgaa tacttattat ctgcaattgt ttttggtgag tttattgggg
     1201 atacgacaac gaataatata agtaaaatta tcgttcgttt aatatagttc acttaaaagc
     1261 tccttgttga agaaatatat gtaaatagtc ttaaattaga attgtaatct ttaataagct
     1321 tgtaagacta aaacatatct taaatattaa agtatgagag tgtgaaatgt ctattaagaa
     1381 taaaaaacag tctgaaacat cattgag
//
LOCUS       DAKTGS010000241         1390 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.250, whole genome shotgun sequence.
ACCESSION   DAKTGS010000241 DAKTGS010000000
VERSION     DAKTGS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.250"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            40..540
                     /locus_tag="O0286_002827"
     CDS             40..540
                     /locus_tag="O0286_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0171132.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     DFTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYEMEEVKQIEQYIKE
                     QEEAEQ"
     gene            551..1046
                     /locus_tag="O0286_002828"
                     /pseudo
     CDS             551..1046
                     /locus_tag="O0286_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            1057..>1390
                     /locus_tag="O0286_002829"
     CDS             1057..>1390
                     /locus_tag="O0286_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0171133.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLK"
BASE COUNT          575 a          125 c          286 g          404 t
ORIGIN      
        1 ttaaagagca agaagaagct gaacaataga ggcgataaca tgaatttcga agaaaaatta
       61 agtgaaatgt acaataaaat tgcaaatgag atcaatgaaa tgataccagt agagtgggaa
      121 aaggtatatg taattgcata tgtggacgat ggaggtgggc aagttatatt ttattatacc
      181 aagcccagaa atgatgagct gtattattat tcgagtatcg ttgaagatta taatgtttta
      241 gaagaaatct ttgatgattt atggatggaa ctttatagat catttaaaaa attaagaaat
      301 atatttaaag aagaaagtct tgaaccatgg acatcatgtg aatttgactt tacaaaagaa
      361 gataaattaa aagtttcatt tgattatatt gattggaaga atacagaatt tgatcaatta
      421 ggccaagaaa actactataa ctatagaaaa ttcggaatta taccagaaat ggaatatgaa
      481 atggaagagg ttaaacaaat cgagcaatat attaaagagc aagaagaagc tgaacaatag
      541 aggcgataac atgattttcg aagaaaaact aaatgaaatg tacaacgaga ttgcgaataa
      601 aattagtagc atgataccag tagaatggga aaaggtatat acaatggctt atatagatga
      661 tggaggaggt gaagtattct ttaattatac taaacagcga tgaattgaat tattacaccg
      721 atatacctaa ggagtataac atttctgtgc aagtatttga tgatttatgg atggatttat
      781 atgatttgtt tgaggaatta agaaatttat ttaaagaaga aggactagaa ccatggacat
      841 catgcgaatt tgattttaca agagaaggta aattaaaagt ttcatttgat tatattgatt
      901 ggataaattc agaatttggt caagtaggtc gacaaaatta ctataagtat agaaaatttg
      961 gaattttacc agaaacggaa tatgaaatta ataaagttaa agaaatcgag caatatatta
     1021 aagagcaaga tgaagctgaa ctataggggc gataatatga ctttcgaaga aaaattaagt
     1081 gaaatgtata gcgagattgc gaataagatt attagcatga taccggtaga gtgggaaaaa
     1141 gtatatgcaa tggcatatat cgatgaagaa tgtggagaag tgttttacaa ttatactgaa
     1201 ccaagcagtg atgaattatt ttactataca agtgtgataa agaagtataa tttattgaaa
     1261 tcaagcttta tggattcagt atatgagttg catgatcaat ttgaggaact aagagaagta
     1321 tttatagaag aaggtcttga accatggaca tcatgcgaat ttgattttac aaaagaagat
     1381 aaattaaaag
//
LOCUS       DAKTGS010000242         1390 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.251, whole genome shotgun sequence.
ACCESSION   DAKTGS010000242 DAKTGS010000000
VERSION     DAKTGS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.251"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            331..789
                     /locus_tag="O0286_002830"
     CDS             331..789
                     /locus_tag="O0286_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0171134.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            782..>1390
                     /locus_tag="O0286_002831"
     CDS             782..>1390
                     /locus_tag="O0286_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0171135.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTD"
BASE COUNT          529 a          148 c          244 g          469 t
ORIGIN      
        1 tttaataata taaaacataa atatgaatac gagtattttt gcagcatgca aatggcattg
       61 ctacatttat taaaatgcgc aatttgttat gagtaacttt tttggatagt aggcacatta
      121 aagtgtcgaa aacaatttgt gtggaagaca gggtaaattc tgcttattta aaatgatagt
      181 ttgtattata ggtggatttg tgaaatgcgg aataaatggt gcataactaa gtaaatatat
      241 gttaattaca acaatatttg acaaaaatgg gagtgacaac aataatatag ttaaccggtt
      301 aactaatgaa gaggaggaat aatagtggat atgaagagag aagttatttt aaaattacaa
      361 caatttatta tagaaagaga aaatgccgat aaaagaagac agtataaaaa ggaaaatgag
      421 gaactagatt tatctctaac acaatttcat atcatagagt tgattgataa taatgataaa
      481 gtgaataata aatttttgtc tgaaatgtta aatgtatcaa aaccagcaat tactaaatcg
      541 ataaaaaaac ttttagcgaa agacttagta gttgaatcgc acaatgaatt taacaaaaga
      601 gaagttaatt attcattaac acaaaaagga aaaaaattat cttatataca tgatgaatta
      661 catgaaaaat cggtaaaaaa atatgaagag gtacttaaag tctttgatga tgatgaaatg
      721 gcagttatta tagagttttt aaaccgtagt atagaagaat taaaaaaaga agatggttta
      781 aatgattaat agtaatatca gattatatat tctaggattt ttagcatttt tcgcatcatt
      841 gattcaaaac atatacacgc ctattattcc gagactatat gacgattttc aagtaccact
      901 attttggata aatgcgacag ttggcggttt tatatttatc gttgcagtta tgcaaattgt
      961 acttggtaga agtatagatt ctcgtgattc gaaaaaagta cttttaacgg gattgggaat
     1021 agtaatcata tctagcttta tttgtgcagt gacacataac tttatattat ttgctatatc
     1081 aaggttattt caagcaattg gttgcggaat tattcctctc gtgactttaa ctttattagc
     1141 caaactaagt acagataatg gtagagctca agctatggcg aactatcaaa tatttttatc
     1201 atgtgcacca gctttggcac caatcttagg gagtacttta ggtgcaagat gggattatat
     1261 tggtattttt agttttttgc tcgttatatc tatcgtatta tttttgatta tcttttttat
     1321 tgatattcca aatgttgaaa aagggattgt aaaattaact gaaaaaattg aagaaaaata
     1381 tttgaccgat
//
LOCUS       DAKTGS010000243         1376 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.252, whole genome shotgun sequence.
ACCESSION   DAKTGS010000243 DAKTGS010000000
VERSION     DAKTGS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.252"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..683)
                     /locus_tag="O0286_002832"
     CDS             complement(<1..683)
                     /locus_tag="O0286_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0171136.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKL"
     gene            complement(685..>1376)
                     /locus_tag="O0286_002833"
     CDS             complement(685..>1376)
                     /locus_tag="O0286_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0171137.1"
                     /translation="VLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTTLM
                     KVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQDID
                     KIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRDVR
                     ELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLENV
                     LLEIIEREDQA"
BASE COUNT          473 a          219 c          183 g          501 t
ORIGIN      
        1 tctaattttg tcgacttact aaagcctttt tcaactgttt gaagcataat attcatcata
       61 ttaataggat tccactttag ccagtctatt ttttctaaaa gtgccgtttg aataactgct
      121 aaaatagaac ttgcaaaata aaaaacaata cctacagcaa tggctactcc cgttgaattt
      181 gttgcagatg ataatagcaa cgttaagctt aaaactaacc aaacgccaac aaatgtgcct
      241 aaactaacta ataataattg attcaataaa gataattgat taccgctact ttcaaatata
      301 tttaaatcat taaagaataa agacccaata acaattgaag caataattgt aataacaaaa
      361 taaattaaag aaataataaa taatgtgatg attttgctaa caatcatagt tgttcttgaa
      421 tattcacgat agagtaaatt tttaatcgta ccgtaatgaa attccattga aatgattgta
      481 cttgcttgaa taattaataa aaatgcaaac catgaaaaac cattataagc agatgtgaaa
      541 gattcctgtg gcgtaaaaat ttcattgtat tttgtagcaa tataaccttg agcaaccatt
      601 agtagaataa agacaatagg tgcgataaaa gttgatttct ttttaaataa tttgaaacat
      661 tcttgtttaa ttaaagttcc cattttatgc ttggtcctcc ctttctatga tttctagtaa
      721 tacattttct aaatcattat ctttaaattg tttaagttct tcttccgatg tttctgtaac
      781 aattttacct ttgttaataa taaggataga gtttgtgatt ttaactaatt cacttaaaat
      841 atgactcgaa attaagaaag taacaccttc ttgcgctttt tggacaatca attcacgtac
      901 atctcgcaca gcttttggat ctaagccatt cattggttca tctaagataa tgaattgagg
      961 tttatttaaa aatgctatag caattcctaa tttttgtttc ataccaagag aatacgtttt
     1021 agctttttta tgaatgtatt catccatatg aagttgtgag acaattttat cgatatcttg
     1081 agtgtttttt gattcattca ataacttcaa gttttcatat ccagacataa aaggatatat
     1141 tcctggattt tcaatcaatg cgccgatatt gcttttgctg tccttgctgt taataacatt
     1201 aaaattcccg ctttggaaac tagagtaacc taatataact ttcattaaag ttgttttacc
     1261 tgcaccattc gctccaacga gaccaactat ttgtccgcgt ttcagcttaa atgatacatc
     1321 ttcgagaatc gttttgttgc ctatcttctt tgttaaatgt tctattgtta aaacat
//
LOCUS       DAKTGS010000244         1355 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.253, whole genome shotgun sequence.
ACCESSION   DAKTGS010000244 DAKTGS010000000
VERSION     DAKTGS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.253"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..1318
                     /locus_tag="O0286_002834"
     rRNA            <1..1318
                     /locus_tag="O0286_002834"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DAKTGS010000245         1332 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.254, whole genome shotgun sequence.
ACCESSION   DAKTGS010000245 DAKTGS010000000
VERSION     DAKTGS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.254"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            30..1286
                     /locus_tag="O0286_002835"
     CDS             30..1286
                     /locus_tag="O0286_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCY0171138.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
BASE COUNT          564 a          150 c          224 g          394 t
ORIGIN      
        1 atatatttta taataggagg gaatttcaaa tgatagacaa ctttatgcag gtccttaaat
       61 taattaaaga gaaacgtacc aataatgtag ttaaaaaatc tgattgggat aaaggtgatc
      121 tatataaaac tttagtccat gataagttac ccaagcagtt aaaagtgcat ataaaagaag
      181 ataaatattc agttgtaggg aaggttgcta ctgggaacta tagtaaagtt ccttggattt
      241 caatatatga tgagaatata acaaaagaaa caaaggatgg atattatttg gtatatcttt
      301 ttcatccgga aggagaaggc atatacttat ctttgaatca aggatggtca aagataagtg
      361 atatgtttcc gcgggataaa aatgctgcaa aacaaagagc attaacttta tcttccgaac
      421 tcaataaata tattacatca aatgaattta atactggaag attttattac gcagaaaata
      481 aagattcatc ttatgattta aaaaatgatt atccatcagg atattctcat ggatcaataa
      541 gattcaaata ttatgatttg aatgaaggat tcacagaaga agatatgcta gaggatttaa
      601 agaaattttt agaactattt aatgaattag cttcaaaagt tacaaaaaca tcctatgata
      661 gcttggtcaa tagcatagac gaaatacagg aagacagcga aattgaagaa attagaacag
      721 cacaaaaaga taagacactc aaggaagtgg aagcacctaa aggaataatt ccaaaatata
      781 aaaaaggtgt atcaaagact actaaaaatg attcagaaat tgaaaaatca aataaagaga
      841 ataaattaac cggtaaagtt ggagaaaaat tagcgctaaa ttactttaat gagctaattg
      901 ataataaaat agacgaagat aagaaagaac agtttaggaa tattttaaat gataatccag
      961 gctctcaaca cggtcatggc tatgatttag tagcttttga tccaacaaat acagataaag
     1021 ctgtagaaaa atttattgaa attaaaacat ctacatcttc tagtattgag gaaccatttt
     1081 ttatgtcgct aaatgaaatg tttgctatga aagaatataa gcagaaatat ttaatattaa
     1141 gaatatttaa tgtttccggt aaagaaccac aattttattt tatagatcca tatgcaaatt
     1201 attctgaatt taaagatgta gatgatctca ttgacaaagt atttaatgta gaagctattc
     1261 agtataaagt ttttggcgaa aaatgattac ttgaacaaga gctaaaataa aattgtgatc
     1321 taataaaaat ag
//
LOCUS       DAKTGS010000246         1303 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.255, whole genome shotgun sequence.
ACCESSION   DAKTGS010000246 DAKTGS010000000
VERSION     DAKTGS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.255"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            23..>1303
                     /gene="trmFO"
                     /locus_tag="O0286_002836"
     CDS             23..>1303
                     /gene="trmFO"
                     /locus_tag="O0286_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCY0171139.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDY"
BASE COUNT          463 a          198 c          273 g          369 t
ORIGIN      
        1 tgacaataaa aggagtatag aaatgactca aactgtaaat gtaataggtg ctggtcttgc
       61 cggttcagaa gcggcatatc aattagctga aagaggaatt aaagttaatc taatagagat
      121 gagacctgtt aaacaaacac cagcgcacca tactgataaa tttgcggaac ttgtatgttc
      181 caattcatta cgcggaaatg ctttaactaa tggtgtgggt gttttaaaag aagaaatgag
      241 aagattgaat tctataatta ttgaagcggc tgataaggca cgagttccag ctggtggtgc
      301 attagcagtt gatagacacg atttttcagg ttatattact gaaacactta aaaatcatga
      361 aaatatcaca gttattaatg aagaaattaa tgccattcca gatggataca caattatcgc
      421 aacaggacca cttactacag aaacccttgc gcaagaaata gtggacatta ctggtaaaga
      481 tcaactttat ttctatgatg cggctgctcc aattattgaa aaagaatcta ttgatatgga
      541 taaagtttac ttaaagtccc gttatgataa aggtgaagct gcatatttaa actgtcctat
      601 gactgaggat gaatttaatc gcttttatga tgcagtatta gaagctgaag ttgcgcctgt
      661 aaattcattt gaaaaagaaa aatatttcga gggttgtatg ccttttgaag taatggcaga
      721 acgcggacgc aagacattac tatttggacc aatgaaacca gtaggattag aagatccaaa
      781 gactgggaaa cgtccttatg cggtggttca attaagacaa gatgacgctg ctggtacact
      841 ctacaatatt gttggcttcc aaacgcattt aaaatgggga gctcaaaaag aagtcattaa
      901 attaattcca ggcttagaaa atgttgatat tgttagatat ggtgtgatgc atagaaatac
      961 cttcattaat tcaccggacg tattaaacga gaaatatgaa ttgatttcac aaccaaacat
     1021 acagtttgcg ggacaaatga ctggtgttga aggttatgta gaaagcgcag ctagcggctt
     1081 agttgcaggt atcaatcttg cgcataaaat attaggcaag ggtgaggtag tatttccgag
     1141 agaaacaatg attggaagta tggcttacta tatttctcat gctaaaaaca ataagaattt
     1201 ccaacctatg aatgctaact tcgggttatt accatcttta gaaactagaa ttaaagataa
     1261 aaaagaacgc tatgaagcac aagctaatag agctttggat tac
//
LOCUS       DAKTGS010000247         1285 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.256, whole genome shotgun sequence.
ACCESSION   DAKTGS010000247 DAKTGS010000000
VERSION     DAKTGS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.256"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            47..226
                     /locus_tag="O0286_002837"
     CDS             47..226
                     /locus_tag="O0286_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171140.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            537..797
                     /locus_tag="O0286_002838"
     CDS             537..797
                     /locus_tag="O0286_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171141.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(850..1200)
                     /gene="scn"
                     /locus_tag="O0286_002839"
     CDS             complement(850..1200)
                     /gene="scn"
                     /locus_tag="O0286_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCY0171142.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          405 a          163 c          169 g          548 t
ORIGIN      
        1 tcaaaaattc attgtaaact tttagacaaa aggaagtaat aaaaacgtga aatttaaaac
       61 aactaaagaa tgtaaaagta ataatatctt taaaagaagt caagaaatta ataatagaga
      121 aagtgaaaag ggttgtttat ggggcattag catgttgatt ctacttttct tattgattct
      181 gtttggaata actgcttgtt catcaagcat tcactttatt aattagattt ttttacttgg
      241 aggtatcatg tgaagaacca tacaaatata attaatatct tattagttat agtcaactca
      301 ttaactcatt ttctaactct aaacacctca ttttttaata attcagcatc ggatttctgt
      361 tttatcatag gggctatatt tttcttaatc ggaatttttg ttgcaatata cggtatgaag
      421 cgagcaacat attggttaaa cttattgatt ttatttacca atatttttta ttttctacac
      481 ttctgtgtgt tacttttgtt aaaatatata ggatttaaat tatttattta tgaagggtgt
      541 gtattgttat ttatctaatt tatagtctaa ttgtctattt catctgcatt ataaattttt
      601 tcataatggc tgcaccattc ttaatgacta atgctgatta tgtatggacg cctatgacta
      661 cagttacgtt atttattttg agtttgatta ttttcttgat atttataaaa acaaaagatg
      721 tcgttcattt aacaattttc atattaaact tacttttttc aatactttat tgtttgccta
      781 tactgtttta tttatgaaca cttttatatt atctagaaaa ttaaaaccac ccgtaaaagg
      841 gtggtttttt taatatttac tttttagtgc ttcgtcaatt tcgttataaa tcttttgaag
      901 ttgatatttt gcttctgaca ttttcttaaa gtcttttgac ttaagagcat acattgcttt
      961 ttgacctgaa atttttatag ttcgcttata ataagtgttt aaacttccag tagctaattc
     1021 attaacattt agttcatcta ataacgattt taattcatta gctaactttt cgttttgata
     1081 ttcattcgat gttggcaagc ttgtgctagc ttgtgcctca tttttatcta gattagttac
     1141 tagtggtgat gctaaaacga ttgctaaagt tcccgcaagt atagattttc taattttcat
     1201 tttttattct ccttagtaat tagtactaat ttattttaat gactgactaa aaaaaagtac
     1261 attaattatt cattaactat ataga
//
LOCUS       DAKTGS010000248         1284 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.257, whole genome shotgun sequence.
ACCESSION   DAKTGS010000248 DAKTGS010000000
VERSION     DAKTGS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.257"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            10..>1284
                     /gene="eno"
                     /locus_tag="O0286_002840"
     CDS             10..>1284
                     /gene="eno"
                     /locus_tag="O0286_002840"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCY0171143.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDG"
BASE COUNT          440 a          213 c          265 g          366 t
ORIGIN      
        1 attataaaca tgccaattat tacagatgtt tacgctcgcg aagtcttaga ctctcgtggt
       61 aacccaactg ttgaagtaga agtattaact gaaagtggcg catttggtcg tgcattagta
      121 ccatcaggtg cttcaactgg tgaacacgaa gctgttgaat tacgtgatgg agacaaatca
      181 cgttatttag gtaaaggtgt tactaaagca gttgaaaacg ttaatgaaat catcgcacca
      241 gaaattattg aaggtgaatt ttcagtatta gatcaagtat ctattgataa aatgatgatc
      301 gcattagacg gtactccaaa caaaggtaaa ttaggtgcaa atgctatttt aggtgtatct
      361 atcgcagtag cacgtgcagc agctgactta ttaggtcaac cactttacaa atatttaggt
      421 ggatttaatg gtaagcagtt accagtacca atgatgaaca tcgttaatgg tggttctcac
      481 tcagatgctc caattgcatt ccaagaattc atgattttac ctgtaggtgc tacaacgttc
      541 aaagaatcat tacgttgggg tactgaaatt ttccacaact taaaatcaat tttaagcaaa
      601 cgtggtttag aaactgcagt aggtgacgaa ggtggtttcg ctcctaaatt tgaaggtact
      661 gaagatgctg ttgaaacaat tatccaagca atcgaagcag ctggttacaa accaggtgaa
      721 gaagtattct taggatttga ctgtgcatca tcagaattct atgaaaatgg tgtatatgac
      781 tacagtaagt tcgaaggcga acacggtgca aaacgtacag ctgcagaaca agttgactac
      841 ttagaacaat tagtagacaa atatcctatc attacaattg aagacggtat ggacgaaaac
      901 gactgggatg gttggaaaca acttacagaa cgtatcggtg accgtgtaca attagtaggt
      961 gacgatttat tcgtaacaaa cactgaaatt ttagcaaaag gtattgaaaa cggaattggt
     1021 aactcaatct taattaaagt taaccaaatc ggtacattaa ctgaaacatt tgatgcaatc
     1081 gaaatggctc aaaaagctgg ttacacagca gtagtttctc accgttcagg tgaaacagaa
     1141 gatacaacaa ttgctgatat tgctgttgct acaaacgctg gtcaaattaa aactggttca
     1201 ttatcacgta ctgaccgtat tgctaaatac aatcaattat tacgtatcga agatgaatta
     1261 tttgaaactg ctaaatatga cggt
//
LOCUS       DAKTGS010000249         1274 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.258, whole genome shotgun sequence.
ACCESSION   DAKTGS010000249 DAKTGS010000000
VERSION     DAKTGS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.258"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..182)
                     /locus_tag="O0286_002841"
     CDS             complement(<1..182)
                     /locus_tag="O0286_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171144.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYK"
     gene            complement(191..619)
                     /locus_tag="O0286_002842"
                     /pseudo
     CDS             complement(191..619)
                     /locus_tag="O0286_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(814..1038)
                     /locus_tag="O0286_002843"
     CDS             complement(814..1038)
                     /locus_tag="O0286_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCY0171145.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(1055..1258)
                     /locus_tag="O0286_002844"
     CDS             complement(1055..1258)
                     /locus_tag="O0286_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCY0171146.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
BASE COUNT          464 a          188 c          177 g          445 t
ORIGIN      
        1 tccttataag taaatcgata atcattcttt tttgccattt cacaaacttc taatatacga
       61 aaatatgcaa aattatacat tttacaaatt tttcttttag aaacaccatg aaatcttaaa
      121 attacaaatt taagataatt taattgattt gaaccatcaa aaaaaggtgt aaactcagtc
      181 ataattatca attcctttca taagaattaa ctgttaacta aagtttacac caccaattaa
      241 aatcatgaat tgtagcgcca aataatccaa atgtatcaat aatgacaccc ataataattt
      301 gacccgcaac tgttgcaatt acagttaatg ttgcacctaa ttttggcaat aacaataaat
      361 tgccagttaa aaagctaacc ccaagcaaac caccgactac ccatgtgtag ttaaatgatt
      421 gattattgta aaagtgaata gtaaatactt ctggattgat aatgatattt aaaataatta
      481 aacaaattgt tccaactgaa aatgaaatga atgaagtata gaaaggcgat ttagtataca
      541 gtgatagtct tgaattgaca gatgtttgga taggaataag cattccaact aggacaccaa
      601 tgatatagaa aagaagcata tgaataaagt ctcactttcc aaagttaata ataaaatcat
      661 atatttacat tatcattata gagaaaatat acaactcatt tacaataatg ttgaaaaaaa
      721 caggacatta ttacaacaaa atcaccctag acaatcatga tgaaagtcta gggtgttagt
      781 atttatacta aattaattgc gctttcttta tatctatttc ttatcttcaa ataaaacagt
      841 tgttgtttta tcaataattt gagcagaatg tactttggtg atagtaccac tatttgactt
      901 tataatattt aaatctacta atgcattcat actatcacgt gcaacttgct cggtcactac
      961 gttgtttaat tgtggtaatt gcagttttac aggcttgtct agtgaagatt taaaacttaa
     1021 ttcaagtgtt ttagtcatga atatgtcctc ctgattaaat tgataaagat ttgatgagtt
     1081 cgatattgct atatgtttct ccagtaagtc gctcaataag tttgctgaat gttttaattt
     1141 ggtcgtttga tgcatcaggg ttaatgttag cgaatcgacg cttaaattct gtttgtttgc
     1201 cgttagcgtc tactttagta aatgataata caatagtgat gtggtttatt ttactcatat
     1261 tttaaaacct cctt
//
LOCUS       DAKTGS010000250         1122 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.259, whole genome shotgun sequence.
ACCESSION   DAKTGS010000250 DAKTGS010000000
VERSION     DAKTGS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.259"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            10..85
                     /locus_tag="O0286_002845"
     tRNA            10..85
                     /locus_tag="O0286_002845"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Val,seq:tac)
     gene            102..177
                     /locus_tag="O0286_002846"
     tRNA            102..177
                     /locus_tag="O0286_002846"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:135..137,aa:Thr,seq:tgt)
     gene            183..258
                     /locus_tag="O0286_002847"
     tRNA            183..258
                     /locus_tag="O0286_002847"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:216..218,aa:Lys,seq:ttt)
     gene            262..343
                     /locus_tag="O0286_002848"
     tRNA            262..343
                     /locus_tag="O0286_002848"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Leu,seq:tag)
     gene            347..421
                     /locus_tag="O0286_002849"
     tRNA            347..421
                     /locus_tag="O0286_002849"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:379..381,aa:Gly,seq:gcc)
     gene            432..520
                     /locus_tag="O0286_002850"
     tRNA            432..520
                     /locus_tag="O0286_002850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:466..468,aa:Leu,seq:taa)
     gene            539..612
                     /locus_tag="O0286_002851"
     tRNA            539..612
                     /locus_tag="O0286_002851"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Arg,seq:acg)
     gene            622..695
                     /locus_tag="O0286_002852"
     tRNA            622..695
                     /locus_tag="O0286_002852"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:656..658,aa:Pro,seq:tgg)
     gene            713..788
                     /locus_tag="O0286_002853"
     tRNA            713..788
                     /locus_tag="O0286_002853"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:746..748,aa:Ala,seq:tgc)
     gene            810..883
                     /locus_tag="O0286_002854"
     tRNA            810..883
                     /locus_tag="O0286_002854"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:844..846,aa:Met,seq:cat)
     gene            912..985
                     /locus_tag="O0286_002855"
     tRNA            912..985
                     /locus_tag="O0286_002855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:946..948,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="O0286_002856"
     tRNA            995..1084
                     /locus_tag="O0286_002856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
BASE COUNT          249 a          266 c          308 g          299 t
ORIGIN      
        1 ttttaaatcg gagaattagc tcagctggga gagcatctgc cttacaagca gagggtcggc
       61 ggttcgaacc cgtcattctc caccatttat tcttacatat tgccggccta gctcaattgg
      121 tagagcaact gacttgtaat cagtaggttg ggggttcaag tcctctggcc ggcaccatgg
      181 aagagccatt agctcagttg gtagagcatc tgacttttaa tcagagggtc agaggttcga
      241 atcctctatg gctcaccatt tgcgggtgtg gcggaattgg cagacgcact agacttagga
      301 tctagcgcct tacggcgtgg gggttcgact cccttcaccc gcatatgcag aagtagttca
      361 gcggtagaat acaaccttgc caaggttggg gtcgcgggtt cgaatcccgt cttctgctcc
      421 atttttatag tgccggggtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggtg
      481 agtgatcacc gtaccggttc gattccggtc ctcggcacca ttttcaataa aaacatatgc
      541 gcccgtagct caattggata gagcgtttga ctacggatca agaggttatg ggttcgactc
      601 ctatcgggcg cgttaattat acgggaagta gctcagcttg gtagagcact tggtttggga
      661 ccaaggggtc gcaggttcga atcctgtctt cccgatatac tgtaattatt atgggggctt
      721 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagt
      781 ctccaccata ttatttacaa actatataag gcggtgtagc tcagctggct agagcgtacg
      841 gttcataccc gtgaggtcgg gggttcgatc ccctccaccg ccactattta ttagttgtaa
      901 aattatattt aggaccttta gctcagttgg ttagagctaa cggctcataa ccgttcggtc
      961 gcaggttcga gtcctgcaag gtccatataa ttttggagga atacccaagt ccggctgaag
     1021 ggatcggtct tgaaaaccga caggggctta acggctcgcg ggggttcgaa tccctcttcc
     1081 tccgttttac taatggtctc gtagtgtagc ggttaacacg cc
//
LOCUS       DAKTGS010000251         1106 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.260, whole genome shotgun sequence.
ACCESSION   DAKTGS010000251 DAKTGS010000000
VERSION     DAKTGS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.260"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            5..940
                     /locus_tag="O0286_002857"
     CDS             5..940
                     /locus_tag="O0286_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCY0171147.1"
                     /translation="MVICPLIFNYTLTLNLTCYILVVISIFLSLIYSIMTYTFPWSMF
                     VTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFISTIQSPIVKGL
                     SMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTEFVTPMLAARLD
                     RALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDILMESHSINTYT
                     NFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGSHTPYHFFLHVL
                     IKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT          352 a          187 c          154 g          413 t
ORIGIN      
        1 acttatcgtt atttgtccgt taattttcaa ctatactctg acgttaaatt taacatgcta
       61 catcttagtt gtcatatcga tatttttgtc actcatctat agcatcatga cctatacttt
      121 cccttggtct atgtttgtaa catttataat aatcagtatt atcatgctat tcaaacatag
      181 atatttattt tctcatactt ctagtcgctt atttattaaa aaattattat ctttttcata
      241 taaatttgta ctctacacat cagcacttct atttataagt acgattcagt cacctattgt
      301 gaaaggtttg tctatgtggt tagctatatt atgtctttcc ctgttactaa cctttatttg
      361 ttatctcgtt tggacagtat catatagaca tagatcatac aacaaagaag ttgatatcat
      421 aattgtgtta ggtgctggta tttttaccga atttgtcaca ccaatgcttg cagcgcgttt
      481 agatcgtgca cttgatatct atcaacaaca agcatcagca acaaagatca ttgtatctgg
      541 cggacaagga ccagacgaac caatttctga agctttagct atgcaacgtt atttgattgc
      601 acatggtgtt gataaaacag atatactaat ggaatctcac tctataaata catacacaaa
      661 ctttttatat tctaaacaaa tcatcaacaa tttgtatcat gaagcagtta aaattgtttg
      721 tgtaacaagt cagtttcatg tattgcgcgc cttacgacta gcccaaaaat taggtttatc
      781 tatcgatggt gtcggaagtc atacaccata tcactttttc ttacatgtgc tcatcaaaga
      841 ttatctaggc gtaatgtatc agtatagatt gttattaacg ttatattgtt caagtagttg
      901 gtttatatgt ctttacgccg cttttattta taatagttaa tccacaaaca aactattatt
      961 ataggttgtt tctaatattg cgtcaaacta tctttaaata ttcgttaatc cttgattaga
     1021 caattgttat attgaaaata aataaactaa agagtctggg acattaatcc ctaaaaaaaa
     1081 cagcagtaag ataattttca attaga
//
LOCUS       DAKTGS010000252         1060 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.261, whole genome shotgun sequence.
ACCESSION   DAKTGS010000252 DAKTGS010000000
VERSION     DAKTGS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.261"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..>1060
                     /locus_tag="O0286_002858"
     CDS             <1..>1060
                     /locus_tag="O0286_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0171148.1"
                     /translation="FKTFPTKIIGLLKLFENNNDPYIVERLYASVLGATLRIDICEIH
                     IEIANYIYEEIFDKEMVYPHILMRDYARQTIEYISLSKDISNINLEKIRPPYKSNWYK
                     KEYSNLNIDDYIKSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVR
                     NWVKGFKSDQDLSNIALMRIFEMGYDAKLHGEFDMWVNRYDNFNNSIERISKKYQWIA
                     YYEILAKLVDKFPDVQYSGLWDDYIRDIDPTLLLLEIDKESKILVPSPLPSHQSNEWV
                     KNTKVFDETKLFLEIDIDNHRYICLSSKFNFEKREKEIPFEDRDSCYFLAMGYFYNKE
                     DSNEIIKGYENNYDRGINI"
BASE COUNT          426 a          106 c          171 g          357 t
ORIGIN      
        1 ttcaaaacat ttccaactaa aattattggt ttattaaaat tatttgaaaa taacaatgat
       61 ccatatatag tcgaaagatt atatgcttct gttttagggg cgacacttcg aatcgacatt
      121 tgtgaaatac atatagagat tgctaattat atttatgaag aaatatttga taaagaaatg
      181 gtatatcctc atattttaat gagagattat gctaggcaaa caattgaata tattagttta
      241 tcaaaagata tatctaatat aaatttagaa aaaattaggc caccttataa aagtaattgg
      301 tataaaaaag aatattcgaa tttaaatata gacgattaca taaaatcttt aaaaaacaaa
      361 ttagatagcc atttacattt ttctatcgat aaaattaaaa actcaatgac aacggaatat
      421 ggaagaggaa ctggggcata tggcgatttt ggcagatatg tatttggtta tgctgtacga
      481 aactgggtaa agggttttaa aagtgatcaa gacttaagta atatagcatt aatgaggatt
      541 tttgaaatgg gttatgatgc aaaattacat ggtgaatttg atatgtgggt taatcgttat
      601 gataacttta ataattcaat tgaaagaatt agcaaaaaat atcaatggat tgcttactat
      661 gaaattttag ctaaacttgt agataaattt cctgatgttc aatattctgg actatgggat
      721 gattatatta gagatattga tcctacttta ctattacttg aaattgataa agaatcaaaa
      781 atactagtac cctcaccact tccttcgcat caatcgaatg aatgggtaaa aaataccaaa
      841 gtttttgatg aaacaaaatt atttttagaa atagatatag acaatcatag atatatatgt
      901 ttaagctcaa agtttaattt tgaaaagaga gagaaagaaa taccatttga agatagagac
      961 tcatgttatt tcttagcgat gggatatttt tataataaag aagatagtaa tgaaattatc
     1021 aaaggttatg aaaataatta tgatagaggt attaatattc
//
LOCUS       DAKTGS010000253         1059 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.262, whole genome shotgun sequence.
ACCESSION   DAKTGS010000253 DAKTGS010000000
VERSION     DAKTGS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.262"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            287..397
                     /locus_tag="O0286_002859"
     CDS             287..397
                     /locus_tag="O0286_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171149.1"
                     /translation="MKMTIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
     gene            825..1010
                     /locus_tag="O0286_002860"
     CDS             825..1010
                     /locus_tag="O0286_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171150.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
BASE COUNT          456 a           95 c          164 g          344 t
ORIGIN      
        1 tttttggtac atttatttga aaatgttttt attacaaagt tacatggcta aatgtttctt
       61 aacaaaatta atttttcgag tgtaaagaat cgtgtggtaa acataattta tatagattat
      121 aaggaggtga aaatggtcga ggaagttaaa acgataaaat agtttgaatg ttttgatttc
      181 aattctggta ggactattga atttgcaatc taataagaat gtgaggtttg atttaatatg
      241 aagtcatatt ctttgaataa aatattactt tctcaccatt agtaaaatga agatgactat
      301 taaagtgcga aaaacaaata aaaaagagaa aatagaattt ttaataggga cgtttataat
      361 tattttagta atattagggt tcaaaattat gaaataaatg tatagtattt gagaagtcta
      421 tatgactccg gaaaaattaa attctaaaga tatctcaata tctcaaattt aatagactac
      481 tggagttagt agtttgaata ttgaaagaca ctaatctaat aaaaatacat aaaaggttaa
      541 actttagaag tggctgtaaa cttcaatgaa tactaaacat tgaagttttg taaaaggcgt
      601 aaggtataca agacaaatcg tttatctttg acaaagtaat tgatgatgaa aagtcgttaa
      661 tatgtagaaa caaaaattga aaatataaaa tttaactatt atgaagtgat gatagccatt
      721 aatgaataga aaaataataa aatcatttat agataaaaaa tacgttaagt tcacagaatt
      781 tcgaaaaaaa gcagaaaaca ttgtacagat atggagatga aaaaatgtca gaatacaaga
      841 aaaaaataat tgaattaatt gaaagtaatt taacaggata tgaaatttct aaaaaaactg
      901 gagtttctca atacgtactt tcacaattaa gacagggcaa acgcgaagta gataatctaa
      961 ccctgaatac aacagaaaaa ttatatgaat atgccaataa agttttgtaa tttaactaat
     1021 gtataaatta atcaagctat gtttatttga tttaactat
//
LOCUS       DAKTGS010000254         1030 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.263, whole genome shotgun sequence.
ACCESSION   DAKTGS010000254 DAKTGS010000000
VERSION     DAKTGS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.263"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            29..277
                     /locus_tag="O0286_002861"
     CDS             29..277
                     /locus_tag="O0286_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171151.1"
                     /translation="MLILKIKEINDKSVTYKYFPNNDENIKPGIIQMDIDSLEVINAE
                     KSSLEKNTRDNYFIHAIDRIYINTSKGLFPESELVAWG"
     gene            312..434
                     /locus_tag="O0286_002862"
     CDS             312..434
                     /locus_tag="O0286_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171152.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            410..508
                     /locus_tag="O0286_002863"
                     /pseudo
     CDS             410..508
                     /locus_tag="O0286_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
     gene            817..954
                     /locus_tag="O0286_002864"
     CDS             817..954
                     /locus_tag="O0286_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171153.1"
                     /translation="MILIMLDSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKD
                     K"
BASE COUNT          422 a          107 c          162 g          339 t
ORIGIN      
        1 tattttaatt aattagaggt ggaaatgatt gctaatatta aaaataaaag aaattaatga
       61 taaaagtgtt acatataaat attttcctaa taatgatgaa aatattaagc ctggtattat
      121 tcaaatggat atagatagtc ttgaagttat caatgctgaa aaatctagtt tagaaaaaaa
      181 tacaagagat aattatttta ttcatgccat agacagaata tacataaata ctagtaaagg
      241 gctatttcct gaatctgaac tcgtagcatg gggataaaga aagtttactt taaaaatttt
      301 aaccttcaaa tgtgaaggtt attttttatt gcccaaaacg tgctgatgac attttaaaag
      361 caagtatgga atatcagtcg acagactata aacggaggta tatctcatgg aaaacaatga
      421 tagcaatgaa gtagcttatt tgttaaaaga tggtaaattt acaaaagtat atgtcaatca
      481 agatagtgta tcttttgtgc cagggtaaca agcaaccgaa ttattattta acagtaaacc
      541 gaattcaatt gttatgttac ataaccatcc tggacagtca ggattttcag aatatgattt
      601 atttacattt tttaagcatc catcaataaa atcaatgaca atcgttacta ataagggaca
      661 agtaaaattt attaccaagt caaataggtt tcatggtaaa atagtgagta aattttgtgc
      721 taaatatttt acgcatatta atatcattaa tgatagccat attgaaaaat tattgaaaaa
      781 actttatagt ataaatatga ttaaatataa agtgagatga ttttaattat gttagattca
      841 caattagatg gaaatgtaac tgcaaaagaa ttgagtgaat cttttaaaga attagttgaa
      901 gaatttgaaa gtatagaaaa agcgaaaaag aattcaaatt caaaagataa atagcatcct
      961 ttttagacag ataaagagaa agtggtgcta tttttatacg cttttttaac tatttactga
     1021 aaatgcagaa
//
LOCUS       DAKTGS010000255         1027 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.264, whole genome shotgun sequence.
ACCESSION   DAKTGS010000255 DAKTGS010000000
VERSION     DAKTGS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.264"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            115..867
                     /gene="sarS"
                     /locus_tag="O0286_002865"
     CDS             115..867
                     /gene="sarS"
                     /locus_tag="O0286_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCY0171154.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT          414 a          150 c          123 g          340 t
ORIGIN      
        1 aaaacacaaa ttttaataaa aatatcatat tatttttaat tgtaaattat ggattatttt
       61 tcttattata ttgtgttata ttaaaataaa gtgcatatac aaggagataa aacaatgaaa
      121 tataataacc atgacaaaat tagagatttt ataatcattg aagcatatat gtttcgtttt
      181 aagaaaaaag tcaagcctga agtcgatatg actataaaag aatttatatt actgacttat
      241 ttatttcatc aacaagaaaa cacacttcca tttaagaaga ttgtttcaga tttatgttat
      301 aaacaatcgg atttagtaca gcatataaaa gtacttgtga aacattcata tattagtaaa
      361 gttcgaagta aaattgatga gcgtaatact tacatttcaa tatctgaaga acaacgagag
      421 aaaattgcag aacgtgttac attgtttgat caaatcatta aacaatttaa ccttgcagat
      481 caaagtgaat cacagatgat accaaaagat agtaaagaat ttctaaactt gatgatgtat
      541 acaatgtatt tcaagaatat tatcaaaaaa catctaacat taagttttgt agaattcaca
      601 attctagcta ttatcacttc tcaaaataaa aacatcgttc ttcttaaaga tttaattgaa
      661 acaatccacc ataaataccc tcaaactgtt agagctctca ataatttaaa aaagcaaggc
      721 tatctaataa aagaacgctc aactgaagat gaaagaaaaa ttttaattca tatggatgac
      781 gcgcagcaag accatgctga acaattatta gctcaagtga atcaattatt agcagataaa
      841 gatcatttac atcttgtttt tgaataatat ctctattacg caagtgtgct gtattctaaa
      901 gtgcacttgt gttttctatt ttttaataaa acctcagcac ataatgaaca actttctatt
      961 ttctatatca cttaaaacca tttccgaaat taaacctcag cacattcaaa gccccacttt
     1021 attctta
//
LOCUS       DAKTGS010000256         1022 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.265, whole genome shotgun sequence.
ACCESSION   DAKTGS010000256 DAKTGS010000000
VERSION     DAKTGS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.265"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..>1022)
                     /locus_tag="O0286_002866"
     CDS             complement(<1..>1022)
                     /locus_tag="O0286_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0171155.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRM
                     WQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWN
                     LKYKQL"
BASE COUNT          298 a          188 c          120 g          416 t
ORIGIN      
        1 tttaattgtt tatattttaa gttccatgta tctgatgtta atccttgtga attaatttta
       61 aatttatgaa tcattctatg tgttttaaac ttatatccaa taaataatga gctagtattt
      121 tgtgttggat aaagcacagt atatgcaggg ctaacaatcc cattataatt tgatttacca
      181 ctagcccctt gccacattct catagaatta tatgcaatat catttttcct aactacttta
      241 taattacttt tatctttact tgaattatct tttctatcca attcactaaa ttttataatg
      301 ccactattta tagttactga aagcatttgc cctttgtcag aacgttcgtt tctctctttt
      361 aaatattttt ctattttgct attttcccaa tctggataat cttcaccatt ctcatctttg
      421 aatcgcagtt cctgtgagaa aattttctgc atatagcctt ttttctgttg ttgaagtaat
      481 tcaagctttt gttcttctaa ttcaatttgt cggtcgagtt tgctgaagaa cttgcctatt
      541 ttttgctgtt cttctaaact tggatattta attagaatag taaaaaaatc attcacagaa
      601 acatttaata atccgtgatt tcttgcaccc tcaactgcaa ttccagaaac ttctctatac
      661 cagtgtgtcg aatcaaaata tgcttccatg aagtctttag acatttcact tttaatagaa
      721 aaacaaatat acaaagagga caatacacca ctatcatatc tagttaatct tttaatagcc
      781 cctaatgggt atccattaga ataacttttg ttatacgcga attctccatt ctttattagt
      841 gtataatttt ctagattttt cgacgaaact gatttactaa aatattctgt ttgatcaatt
      901 aaacctaact gtccggatat tgttaaaggc tttttcgatt ctaagttttt atttttccta
      961 attactctat ctgtaagatc ccctaactgc ttctcttccc attcgccttc aaaccctggg
     1021 aa
//
LOCUS       DAKTGS010000257         1009 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.266, whole genome shotgun sequence.
ACCESSION   DAKTGS010000257 DAKTGS010000000
VERSION     DAKTGS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.266"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..>1009)
                     /locus_tag="O0286_002867"
     rRNA            complement(<1..>1009)
                     /locus_tag="O0286_002867"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          230 a          298 c          196 g          285 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaaagc tatttcggag
      601 agaaccagct atctccaggt tcgattggaa tttctccgct accctcagtt catccgctca
      661 cttttcaacg taagtcggtt cggtcctcca ttcagtgtta cctgaacttc aacctgacca
      721 agggtagatc acctggtttc gggtctacga ccaaatacta aacgccctat tcagactcgc
      781 tttcgctacg gctccacatt tactgcttaa ccttgcatca aatcgtaact cgccggttca
      841 ttctacaaaa ggcacgccat cacccattaa cgggctctga ctacttgtaa gcacacggtt
      901 tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac ggtactggtt
      961 cactatcggt cactagagag tatttagcct taggagatgg tcctcccag
//
LOCUS       DAKTGS010000258          976 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.267, whole genome shotgun sequence.
ACCESSION   DAKTGS010000258 DAKTGS010000000
VERSION     DAKTGS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.267"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..130)
                     /gene="secG"
                     /locus_tag="O0286_002868"
     CDS             complement(<1..130)
                     /gene="secG"
                     /locus_tag="O0286_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCY0171156.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFG"
     gene            complement(197..655)
                     /locus_tag="O0286_002869"
     CDS             complement(197..655)
                     /locus_tag="O0286_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171157.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
BASE COUNT          355 a          167 c          124 g          330 t
ORIGIN      
        1 taccgaataa ctgctcagca ccaccactga tggcacctga aagtccactg cttttacctt
       61 cttggagtag tacaacagtt attaatgcaa tacaatcaat gattaataat acgattaaaa
      121 atgtatgcat aaattgtcct ccgttcctta tatacgacaa gtagtatatc atatttttat
      181 tgttaaaatc aattttctag cgattaaatg tacttttacg tttgatagaa acaacaagca
      241 tataaatcgc taatgcaacc gaacctataa taacaattaa aattggcaat gttaagtacg
      301 caccgtgtgc agcttttatt gcttgaattg tgattgataa attaatcaac gtaaacaaac
      361 tatttgatac ataaactgca aacataaacc accacacata agtatgtcga ttccaaaaag
      421 tgatgaagcc tgctagataa gccaaaatat acataatacc agtgaatcta aagctgttta
      481 aaatgtaatc aatcgcttta tctgaagtgt tttgattcgt taaattaagt aatgctttgg
      541 cagtcgcatt atccaacgaa acaaagtaat gaataatcaa taatattgag aatacgattg
      601 atgcagttgc aataaagcgt cctgttttaa tttctttatt cgttctatca gccatttgtt
      661 tactcctcat atatttaaat acgttctata aaacctttat ttaattttac cttttaataa
      721 aagtgaatac cctgtcatac aatattcatt tcaacataaa tttattatac cagtttaaga
      781 atacaagtta aatatgtgtt attagttacc tcatgattat tagtctgtta tgattaatac
      841 ttcaaaataa aaaacctacc acagtctcaa ctctgtggca ggctataaat ttaagcgtga
      901 acttatttaa gcgtgaactc aaaatcttta tttaatttat accgtcataa taatcctcaa
      961 ctaaaattat gtcagc
//
LOCUS       DAKTGS010000259          963 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.268, whole genome shotgun sequence.
ACCESSION   DAKTGS010000259 DAKTGS010000000
VERSION     DAKTGS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.268"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(104..>963)
                     /locus_tag="O0286_002870"
     CDS             complement(104..>963)
                     /locus_tag="O0286_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0171158.1"
                     /translation="QNPPKRVFQSLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAG
                     SKIIGQTDAKKQMSIDLYKHTSLENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITR
                     IQFSMMQNDESFNYIFGDEHAKVSLSKDLVDRETNVVLIDEFDKVLPIYYNVFYQMFD
                     EGIIEDANYKVDVSNCIFILTSNFNSIEEAIVNIGEPVFSRIDSCIKFLYLTQEEKQK
                     VIENKLNEILSSLNEKEKRIVTAYNLLEKYKETEIDIDNIRYLKKIITNDIYTIIFED
                     ELFNTDIID"
BASE COUNT          321 a          137 c           95 g          410 t
ORIGIN      
        1 aattttaaaa ttattaaact cttaacttat actacttaca accttacata gatagtactt
       61 tattaataga taacttatac taaaaaaata taaagcatat aaattaatct ataatatccg
      121 tattaaataa ttcatcttca aaaattattg tataaatatc atttgttatt attttcttta
      181 aatacctaat gttatcgata tctatttctg tctctttata tttctctaac aagttatatg
      241 cggtaactat tcttttttct ttttcattta gagatgatag tatttcattt aatttatttt
      301 ctattacttt ctgcttttct tcctgtgtta aatataaaaa tttaatacag gaatctattc
      361 tagaaaatac cggttctcca atattaacta ttgcttcttc aatactatta aaattagatg
      421 ttagaatgaa aatacaattt gaaacgtcta ctttataatt ggcatcttca ataatacctt
      481 catcaaacat ttgatagaaa acattgtaat agattggtaa taccttatca aattcatcaa
      541 ttaaaacaac attagtttca cgatctacta aatcttttga caatgatact ttggcatgtt
      601 catctccaaa gatataatta aaagattcat cattctgcat catagaaaat tgtattctag
      661 tgatattacc tttatatttt tttgctattt ctttggctaa ttctgttttt ccaacacctg
      721 atggaccata gtaaagcatt acggtcggtt tattattttc taaggaagta tgtttatata
      781 aatctatgct catttgtttt ttagcgtctg tttgacctat tattttagaa ccagccatct
      841 gctctttata aaaatttatt acatcatctt tttttaatgt tgtatattca tagtttattt
      901 ctattatttc agaatcgtga aaactattta gtgattgaaa tactcttttt ggaggattct
      961 gaa
//
LOCUS       DAKTGS010000260          949 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.269, whole genome shotgun sequence.
ACCESSION   DAKTGS010000260 DAKTGS010000000
VERSION     DAKTGS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.269"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(63..151)
                     /locus_tag="O0286_002871"
     tRNA            complement(63..151)
                     /locus_tag="O0286_002871"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Ser,seq:gct)
     gene            complement(154..228)
                     /locus_tag="O0286_002872"
     tRNA            complement(154..228)
                     /locus_tag="O0286_002872"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Asn,seq:gtt)
     gene            complement(370..939)
                     /locus_tag="O0286_002873"
     CDS             complement(370..939)
                     /locus_tag="O0286_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0171159.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT          317 a          157 c          156 g          319 t
ORIGIN      
        1 ctcattcatt atatataagt aaacaaaaaa gctaaaatgg tatataccat tttagcaagt
       61 aacggaaacg gagggattcg aaccctcgcg ccgctttcgc gacctacacc cttagcaggg
      121 gcgcctcttc agccaacttg agtacgtttc caatggctcc acaggtagga ctcgaaccta
      181 cgaccgatcg gttaacagcc gatagctcta ccactgagct actgtggatt aatttgtatg
      241 tgtttgaata tcaagcacaa atattattat aacaagatta agttaatttg caatagatac
      301 cttaaaaaaa tatgcagaac atttacacta tatttataaa acattccgca tattttaaaa
      361 ataaacaatt tatttatctt caaataaaga atagcgtgtc aatgcttcaa acacattcaa
      421 tgtggcttta ttataatcta ttggttggtc gggatttttt gatatcatgc gagattgttt
      481 atctaccatg taatagtaaa aaatggaatt gttatattga tgccataaac tgtggtatga
      541 aacatgaaga atgattgatt cattattaat aaatgtcact ttacatttac gattttttaa
      601 ttctttaata ctttcgatat aatgcatatt taaccaaata ttttcatttt gtctgtcaga
      661 atgtgttggg aaaaaataag ttggaaataa tggtgttagt aaaataggtg gtttactaga
      721 aatgccagta atgcggtttg tctctgcttt cttgccaaga taagtattac cataaaattt
      781 gcaagagcgt tcaatgattt tctggacttt aaaaggattt tgaatacgcg ttttgtcaaa
      841 ccgaattatt tcactaccgt ttctttgatc gtcatcatca aatgctggtc gaataaccat
      901 gtctcctttg cgtatcacat aaatattttg agaatacata agcaaaacc
//
LOCUS       DAKTGS010000261          893 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.270, whole genome shotgun sequence.
ACCESSION   DAKTGS010000261 DAKTGS010000000
VERSION     DAKTGS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.270"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            234..734
                     /locus_tag="O0286_002874"
     CDS             234..734
                     /locus_tag="O0286_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0171160.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            746..>893
                     /locus_tag="O0286_002875"
     CDS             746..>893
                     /locus_tag="O0286_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCY0171161.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
BASE COUNT          341 a           89 c          183 g          280 t
ORIGIN      
        1 gagttttagg gctattattt acaaataata taatactgca aagactcgaa attatttaga
       61 attccagtta tcgtaacaat agttgcagtt attttagttc cattagtgta tttaggttta
      121 aaatgagtaa tattaatttg aagaagcatt aagcgacgta gaggtgttgt ttagtgcttt
      181 ttatttaacg gaaatgaaac caatatctat tttgaaaata taggggcgat aacatgactt
      241 tcgaagaaaa actaagtgaa atatacaaca agattgcgaa tgagattagt gggatgatac
      301 cagtagaatg ggaccaagta tttacgatag cttatgtaaa tgatagaggt ggagagatcg
      361 tttttaatta tactaaacca ggtagcgatg aattgaatta ttacacatat atccctagag
      421 agtataatgt ctctgaaaaa gtattttatg atttgtggac ggatttatat agattgttta
      481 agaagttaag aaacgcattt aaagaagaag atttagaacc atggacatca tgtgaatttg
      541 actttacaag agatggtaaa ttgaatgttg tatttgatta tgttgattgg atgaattcag
      601 aatttggtcc aatagcaaaa gaaaattatt atatgtacaa aaagtttggt gttttaccag
      661 aaacggaata tgaaattaat aaagttaaag aaatcgatca atatgttaaa gagcaagatg
      721 aagctgaact atagggggag ataacatgac tttcgaagag aaactaagtc aaatgtacaa
      781 taaaattgca agtgagatca gtggaatgat accagtagaa tgggagcaag tatttacaat
      841 agcttatgta actgatcaag ctggggaagt catttttaat tatactaaac cag
//
LOCUS       DAKTGS010000262          891 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.271, whole genome shotgun sequence.
ACCESSION   DAKTGS010000262 DAKTGS010000000
VERSION     DAKTGS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.271"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..79
                     /locus_tag="O0286_002876"
     CDS             <1..79
                     /locus_tag="O0286_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0171162.1"
                     /translation="GVLPETEYEMEEVKEIEQYIKEQEE"
     gene            289..>891
                     /locus_tag="O0286_002877"
     CDS             289..>891
                     /locus_tag="O0286_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0171163.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSAFIYIERIIEYILVPNVVESKFVIMVSIIIIAC"
BASE COUNT          331 a          105 c          141 g          314 t
ORIGIN      
        1 tggtgtttta ccagaaacgg aatatgaaat ggaggaggtt aaagaaatcg agcaatatat
       61 taaagagcaa gaagaataga caatcttaat gtaagattaa acaataaagc tttgtttagt
      121 ctttttagcg tttaagtaaa aaacaataga gaccataaag ttgtcatcaa ataataatat
      181 aaagataatt ttaggctttt atacttttaa tcgaaaagaa tctaagtact tataacttta
      241 aatacttaat atgctattac aaaatcaatg tataggagat aggtaaatat gactactaag
      301 gaaaatatag acattcttcg aaagccaggt gcacaagcct taagtttagc atcattattt
      361 atgatacttt tttcatgtct aactttcttt tttggtttag attatgaaag gtttccaaat
      421 tatttaaaga taacgacaat tatagaatta ataattatca taattagttt acttcaatgg
      481 attagattta tagatttcga aaaggaaagc gcacagaaat ataagaaaat atatgcccga
      541 tttttagtta ttataaatgt gctaactact atcaccgcag tatttgcaac atgtaacctt
      601 tattattttg ttgctgtaca aaatcattat gacctattca attattggtt gatgggtacg
      661 atttcaatca taattagtta tttgttatta gtaattggcg gaatgttcac gttattaaaa
      721 ttacctaaag taacaaaacg ttggggtggt aaaactaaaa cacatttcgg tttattatta
      781 accgcgttga gcgcatttat atatattgaa agaattatcg aatatatatt ggtgcccaat
      841 gtcgtagaat ccaagtttgt cataatggta agtatcatta ttattgcttg t
//
LOCUS       DAKTGS010000263          853 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.272, whole genome shotgun sequence.
ACCESSION   DAKTGS010000263 DAKTGS010000000
VERSION     DAKTGS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.272"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            96..695
                     /locus_tag="O0286_002878"
     CDS             96..695
                     /locus_tag="O0286_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0171164.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT          329 a          124 c          151 g          249 t
ORIGIN      
        1 atataattgg atataacaaa taaataataa ttattgcaaa acacacccga aattaattat
       61 tataaaagta tattcataaa aggaggaata tacttatggc atttaaatta ccaaatttac
      121 catatgcata tgatgcattg gaaccatata tagatcaaag aacaatggag tttcatcacg
      181 acaaacatca caatacgtac gtgacgaaat taaacgcaac agttgaagga acagagttag
      241 agcatcaatc actagcggat atgattgcta acttagacaa ggtaccggaa gcgatgagga
      301 tgtcagtccg taataatggc ggtggtcatt ttaaccattc attattctgg gaaatactat
      361 cacctaattc tgaagaaaaa ggtggcgtaa tagatgacat caaagcgcag tggggcactt
      421 tagatgaatt taaaaatgaa tttgcaaata aagcaacaac attatttgga tcaggttgga
      481 cttggttagt tgttaatgat ggcaaattag aaattgtgac aacgccaaac caagataatc
      541 cattaacaga aggcaaaaca ccaatcttac tatttgatgt ttgggagcat gcctactatc
      601 tgaaatatca aaataaacgt ccagactata tgactgcatt ttggaacatt gttaactgga
      661 aaaaagttga tgaattatac caagcagcaa aataatataa cttaatataa ttgaggtgga
      721 gcatctacaa ggtgttccac ttttttgtac ttaatatctt tacttatttg tttttgtgta
      781 atgggatagc acgtaaaagt ggaaaaataa taagataaat ttcaactcga aataccttat
      841 tattgttgac tat
//
LOCUS       DAKTGS010000264          832 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.273, whole genome shotgun sequence.
ACCESSION   DAKTGS010000264 DAKTGS010000000
VERSION     DAKTGS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.273"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            13..>832
                     /locus_tag="O0286_002879"
     CDS             13..>832
                     /locus_tag="O0286_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0171165.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISEN"
BASE COUNT          352 a           92 c          136 g          252 t
ORIGIN      
        1 gggtgaagta atatgattcg aattcaagat aatacaataa gggatggtat gcaacaaagt
       61 aatgttcgaa aaagtctaat tataaaaaaa gaagtattga aacaaattaa caagttaaat
      121 ataaattctg ttgaagtagg catgtgtaca actatcgagg atgaatttaa tattcatcaa
      181 ttcagagaca ttttaagtcc tgaaaaagaa ttagtagtat tgactaggct taatgaaaaa
      241 gaaataaaaa aaatagtcaa attgaaaatt cataatttag tggtaaaaat actattgcca
      301 atatctgact tgcatataaa agaaaagctt aatttttcaa ataaatatta tattcagaaa
      361 atcaaagact gcttggatat attaaagaaa gataaaaaag gagtagatat ttgttttgaa
      421 gatggaacaa ggacttctag agaaaaattg aaagaataca tggaaattat ttcaaaatat
      481 caagttagaa cagttacatt tgcggacact gtaggatgtt cgacaccatt agaatacgga
      541 gatattttta attactttgt aaaaaaatat tctaacataa ttttttctgc tcattgtcat
      601 aacgatctag ggttggctac tgcaaataca ttagctgcaa ttttaaatgg tgcaaagcaa
      661 atagaaacta catttttgag aattggtgag agagcgggta atgctcctat tgaggaaata
      721 attactattt tgacaaaaaa acaaatagaa agtacggaat tcactttacc cgacgtatat
      781 aaaactagta ttaatatttc taaaattctc gattttcaaa tatcagaaaa ca
//
LOCUS       DAKTGS010000265          819 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.274, whole genome shotgun sequence.
ACCESSION   DAKTGS010000265 DAKTGS010000000
VERSION     DAKTGS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.274"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            491..>819
                     /locus_tag="O0286_002880"
     CDS             491..>819
                     /locus_tag="O0286_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCY0171166.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKQNFGEDISNVDIFVVREN
                     SDRSGNF"
BASE COUNT          341 a           76 c          144 g          258 t
ORIGIN      
        1 ataggtgatt tctttaaaaa aatggatata ttgataagta aacagaaaat gaaaattgaa
       61 atattagaaa aagagaaaca atccttttta caaaaaatgt tcttataact ttgataaata
      121 catagattgc ataagaataa aatttgtata atttaacata aaagttgtaa aagtaaagtg
      181 aattaaaaac gaacattaaa tttaggcact gtgaaagcgc agtgtctttt ttgtgtcgaa
      241 attgtgtaca gaataagtag ttaaataaag attaagttga gataaagtgt tattcgtaaa
      301 taaaagagag tagatcgata ggaattgaat gatattagtt aactatttat taaattactt
      361 aataatgatt aatttttagt taaagtaagt ttaatgtgaa gcacgaccat tgctcattat
      421 aatgaatgag gattgttcgt attgcgtaat agaataaatc aaatagacta aaaattggga
      481 gcatagaatt atgaaattaa aaaatattgc taaagcaagt ttagcactag ggattttaac
      541 aacagggatg attacaacta ctgctcagcc agtaaaagca agtacattag aggttagatc
      601 acaagctact caagacttga gtgaatatta taatagaccg ttctttgagt atacaaatca
      661 gtcaggatat aaagaggaag gaaaagtgac gtttactcct aattatcaac ttatagatgt
      721 aactttaact gggaatgaaa agcaaaattt tggtgaagat atttctaatg tagatatatt
      781 tgttgtaaga gaaaattctg atagatctgg taacttcaa
//
LOCUS       DAKTGS010000266          814 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.275, whole genome shotgun sequence.
ACCESSION   DAKTGS010000266 DAKTGS010000000
VERSION     DAKTGS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.275"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..605)
                     /locus_tag="O0286_002881"
     CDS             complement(<1..605)
                     /locus_tag="O0286_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171167.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFW"
     gene            complement(598..>814)
                     /locus_tag="O0286_002882"
     CDS             complement(598..>814)
                     /locus_tag="O0286_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCY0171168.1"
                     /translation="GTADDILITENEAIKHMGENVRYMLRSQNKTNYKTSSTIIKVTD
                     NKIELLREGDIKFEEIKERLGTGIIYE"
BASE COUNT          280 a          130 c           79 g          325 t
ORIGIN      
        1 atccaaaact tctcttctgg tagaaatttc tttatctttc cctttgtttc tcctattaaa
       61 tttaattccc tattttcaac taatgtgcat cgaataatta taattgttag aattgaaaat
      121 ataccagtca ttattatagg ccatttaata tttattccat aaagataact acctataatc
      181 cctgaaatta ataatgatag gaacatatag ctatttgact tattttgtac gtatttctca
      241 tttgttatat ttcttttaat tattgcggta tcaacacccg ccatcattga gtaacttaac
      301 cctaataata tttgtgccac tactaaaatt aaatattgat tttgatataa taacaacaat
      361 aaaccgatga ttttgaatat ttcactaaca actaatttat tagaatcttt taagttacaa
      421 attttaaaac acttctcttt gtatagagag aataaaaatg ctgcaatgcc ataagacgcc
      481 attaatattt ctaattgtat tatggaataa ccttgaatca aaaagtaaat taataaaaac
      541 ggtaaataaa agtatgctcg tgaaaaaatt cggtataaat aaatcccaag tattaactta
      601 ttcataaata atacctgtac ctagtctttc ttttatttct tcaaacttta tatctccttc
      661 tcttaataat tcaattttat tatctgtcac tttaataatc gtactagatg ttttatagtt
      721 agttttattt tgacttctta gcatatatct tacattttcg cccatgtgct taattgcttc
      781 attttcagtt attaaaatat catcggcagt tcca
//
LOCUS       DAKTGS010000267          808 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.276, whole genome shotgun sequence.
ACCESSION   DAKTGS010000267 DAKTGS010000000
VERSION     DAKTGS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.276"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..>808)
                     /locus_tag="O0286_002883"
     rRNA            complement(<1..>808)
                     /locus_tag="O0286_002883"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          170 a          242 c          173 g          223 t
ORIGIN      
        1 aaggatgtca agatttggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggtccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac
      181 tcatcgttta cggcgtggac taccagggta tctaatcctg tttgatcccc acgctttcgc
      241 acatcagcgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc tccatatctc
      301 tgcgcatttc accgctacac atggaattcc actttcctct tctgcactca agttttccag
      361 tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa aaaaccgcct
      421 acgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct
      481 gctggcacgt agttagccgt ggctttctga ttaggtaccg tcaagatgtg cacagttact
      541 tacacatatg ttcttcccta ataacagagt tttacgatcc gaagaccttc atcactcacg
      601 cggcgttgct ccgtcaggct ttcgcccatt gcggaagatt ccctactgct gcctcccgta
      661 ggagtctgga ccgtgtctca gttccagtgt ggccgatcac cctctcaggt cggctatgca
      721 tcgttgcctt ggtaagccgt taccttacca actagctaat gcagcgcgga tccatctata
      781 agtgacagca agaccgtctt tcactttt
//
LOCUS       DAKTGS010000268          789 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.277, whole genome shotgun sequence.
ACCESSION   DAKTGS010000268 DAKTGS010000000
VERSION     DAKTGS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.277"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
BASE COUNT          273 a          130 c          103 g          283 t
ORIGIN      
        1 cctaaaaagt agaaacacca gcatcgcaac aataaaataa acaggaataa aagctttctt
       61 ccaaaatata gaatctttca attaagtcac tccttatttt gataacactc ttaaatcatg
      121 gttcttgagt tatcatctgg ttatcatccg atagctcttt cccttgaata tctattatta
      181 gaacactatc gtacggtctc cgcaagctaa atgacaattt aataatgtac acttttgatt
      241 gtgtaagcat gtacaaatta tacgtgtaaa tcaataattc atagttgaat taattctata
      301 cctgagacat taattcattt atactctatt ccatattccc cacacaaacg caaaaaaaca
      361 cctcgacata acatcgaggt gttttgtata tatatcgtcc gacaattaac gttttgagaa
      421 ttgaggtgaa cgacgagctg ctttaagaat ttgttttttt acgttcagaa aattttagaa
      481 tttcgcatga ttatatagac tgtcgtacac cttgatatta aaggatttct tcaattttga
      541 atttcattga ttttcatcaa attgaagcaa agggtattaa cggggtatta attttttaaa
      601 aaaatctgct ggtcggtgga ttcaataatt gatcttattt actgtatttg acttctatac
      661 ttttgaaaaa attttctata tcaactttat ccatatttaa aatatcttta ttgaacacac
      721 aatgcaaata tggttttgca gaaaaaaaag tattgccttc acttatcagt tgataattaa
      781 catccttaa
//
LOCUS       DAKTGS010000269          787 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.278, whole genome shotgun sequence.
ACCESSION   DAKTGS010000269 DAKTGS010000000
VERSION     DAKTGS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.278"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(29..499)
                     /locus_tag="O0286_002884"
     CDS             complement(29..499)
                     /locus_tag="O0286_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCY0171169.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            566..>787
                     /locus_tag="O0286_002885"
     CDS             566..>787
                     /locus_tag="O0286_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0171170.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQL"
BASE COUNT          247 a          142 c          119 g          279 t
ORIGIN      
        1 acaaatttta attagtatgt actatcgtct aattggtgga tttcttattg gctcttaagt
       61 ttttaaaaaa tgttgttaat aatgtgctac atgcttcttt aagtacacct ttatcaacaa
      121 ttgcacgatg attaaaatta gattgttgca ataaattcat taaactgcca ctacaaccac
      181 ctttaggatc atctgcgcca tagacgactc ttggaatgcg actcattaca attgttcctg
      241 cgcacatgac acatggttct aaggttacat ataatgtgca accttctaaa cgccaactac
      301 ctaacacttt ggctgcacgt tcaattgcaa tatgttcagc atgcgccgtt ggttgttgta
      361 gtgtttctct taaattatgt gctctagcga taacttcatc atctttagtg atgatagcac
      421 ctataggtac ttcgcctagt tgagctgctt ttttagcttc ttcaatcgct aatgtcataa
      481 aatatatatc atttgtcatt tatgtccaga tacctcactt atggtacaat actcaatgtc
      541 tattttaaca tttggagatg tgaatatgaa caaacctttt atagcaattg aaggtcctat
      601 tggcgtaggt aaatcttcac ttgcacacaa attaagtcaa actttagatt tttatgaaga
      661 aaaagaaatc atcacagaaa atccattttt atcagacttt tatgaagata tctctaaatg
      721 gagttttcaa actgaaatgt tctttttatg caatagatat aagcaatttc aagatgtaac
      781 acaacta
//
LOCUS       DAKTGS010000270          784 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.279, whole genome shotgun sequence.
ACCESSION   DAKTGS010000270 DAKTGS010000000
VERSION     DAKTGS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.279"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            270..656
                     /locus_tag="O0286_002886"
     CDS             270..656
                     /locus_tag="O0286_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCY0171171.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            649..>784
                     /locus_tag="O0286_002887"
     CDS             649..>784
                     /locus_tag="O0286_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCY0171172.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     L"
BASE COUNT          264 a           86 c          149 g          285 t
ORIGIN      
        1 ctgtgttggg gccccgctat aattgaaaag tttgttatag gtgtattttc ttttggttaa
       61 ctattgttaa tataacattg tagattttag gatgttgatt tttgacttag cctttgtctg
      121 cttatgttaa atgttccagc tagtcaaaca ttgagtttgt aattattata ttaagctagt
      181 ttaaattttt tgtgatttat gttttattca taccaatttt gtgaaatgat acagtgacag
      241 atgtttattg attttaaagg gtgatgatga tggctattgt aaataaagtg ataattgttg
      301 aaggaaaatc tgataaaaaa agggtgcaac aggttattgc agaaccagtc aatattattt
      361 gtactcatgg aacaatgagt atagataagc ttgatgatat gatagaatca ctgtatgata
      421 aacaagtttt tgtattagcc gattctgatg acgaaggaga tcgaattaga aattggttta
      481 aacgttattt gagtgaaagt gaacatatat ttattgataa aacttactgt caagttgcga
      541 attgccccaa acaatatttg gcgcatgtac tttcaaaaca tggctttact tgtaagaaag
      601 aaacacctct tttaccgaat ataaataatg aaaggttagt tttagtaaat gaataattca
      661 ttagacatca aagatgtaac tacattttat gaggaagaca aacatttaat ctttggttat
      721 acaccaacgt gtggtacttg taaggtttca gaaagaatgt tagacattgc taatgaaata
      781 ttgc
//
LOCUS       DAKTGS010000271          776 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.280, whole genome shotgun sequence.
ACCESSION   DAKTGS010000271 DAKTGS010000000
VERSION     DAKTGS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.280"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..173
                     /locus_tag="O0286_002888"
     CDS             <1..173
                     /locus_tag="O0286_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0171173.1"
                     /translation="RYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEKKEENIYF
                     SDSLDYKKSGDV"
     gene            225..>776
                     /locus_tag="O0286_002889"
     CDS             225..>776
                     /locus_tag="O0286_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0171174.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSA"
BASE COUNT          335 a           75 c          150 g          216 t
ORIGIN      
        1 aaagatatga tataccgacg agtaaagctc caaagttatt gttaaaaggt tcagggaatt
       61 taaaaggctc atcagttgga tataaagata ttgaatttac gtttgtagag aaaaaagaag
      121 aaaatatata ctttagtgat agcttagatt ataaaaaaag cggagatgta taatcatggc
      181 tcaatcagaa tatgaaatca atcccggaaa aagagagtga tgaaatgata aaacgtgtaa
      241 ataaattagt gcttggtatt agtcttctgt ttttagtcat tagtatcact gctggttgtg
      301 gcatgggtaa agaagcggaa ataaagaaaa gttttgaaaa aacattgagt atgtatccga
      361 ttaaaaatct agaggattta tacgataaag aaggatatcg tgatgatcaa tttgataaaa
      421 atgataaggg aacatggatt gtaaattctc aaatggcaat tcaaaataaa ggagaagctc
      481 taaaaataaa aggcatgctt ttgaagatag atagaaatac aagaagtgca aaaggatttt
      541 actatactaa tgaaataaag acggagaaat acgaagtagc tcaggataat caaaaaaaat
      601 atccagttaa aatgattaat aataaattca tttctactga ggaagttaaa gaagaaaaca
      661 taaaaaaaga aatcgaaaac tttaagtttt ttgcgcaata tagcaatttt aaagatttaa
      721 tgaattataa agatggagat atatcatata atccagaggt gccgagttat tcagct
//
LOCUS       DAKTGS010000272          771 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.281, whole genome shotgun sequence.
ACCESSION   DAKTGS010000272 DAKTGS010000000
VERSION     DAKTGS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.281"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..598)
                     /gene="sdpA"
                     /locus_tag="O0286_002890"
     CDS             complement(<1..598)
                     /gene="sdpA"
                     /locus_tag="O0286_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCY0171175.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFL"
BASE COUNT          309 a          111 c          102 g          249 t
ORIGIN      
        1 ttaagaagtg catgaatatt acaacttcta atcgttttgt ttttatataa attaggccaa
       61 aaatcaaacc agaatataag taaggtaaat aaccaatcca agtgtcagat tcgtgtaatg
      121 atgcaaaaac taaactagac acaattaacc ctaaaaacaa gtgttttctg aagataaccc
      181 ttattatcat accgcgaaaa acaatttctt ccacaatagc ggggatgatg gtaacagtga
      241 aaatagaaat atagaatggt gtatttcgta ttgcttgctc taattgttgt tcatttgccg
      301 gtacgtcatg aaaatataaa taaagattat ctaacccata aattaatagt gcaccgccta
      361 taatataaat aacttgagac aatgttaatt ttttaaaaga gaataaattg attttgaata
      421 gcctcaatag aattaaacct actaatacaa ttagcatgct ataaagtaaa tgtacttcac
      481 tgacaactat gccgataaat aaaatgatag taataaaaaa tgctgccatt aaagttaaaa
      541 aataatttcc tcgtgtaacc gataaatcat cgtaaaaaaa gtctttgaat ttttgcatgc
      601 taaatccttt cttttcattt tttaaaatta ttttgaatgc aatatgatta aggctagatt
      661 cataatatat gaaacatatt tacgattaaa atttatcatt gtacctacct aaaaagcatt
      721 agtttgaaaa attagcagac tgtattttag cgtcaaatca ccataaatga a
//
LOCUS       DAKTGS010000273          749 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.282, whole genome shotgun sequence.
ACCESSION   DAKTGS010000273 DAKTGS010000000
VERSION     DAKTGS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.282"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(17..658)
                     /locus_tag="O0286_002891"
     CDS             complement(17..658)
                     /locus_tag="O0286_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0171176.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMNMHSIIDKKVHIFLKSNK"
BASE COUNT          290 a          110 c           79 g          270 t
ORIGIN      
        1 ctaactaaat ttgtaactat ttattagact ttaagaatat atgtactttt ttatctataa
       61 ttgaatgcat attcataaac ataaatagca tagtcatgaa aagccaagaa acatataaga
      121 ttaaattttg cacattttca aataccaaca agtaaaatgc cccataagac atgaaaccag
      181 taaataggta ataaaaaatt gtgaaacaaa tatttttaaa ggagggtaat aatcttaatt
      241 taagttcatt atttttactt tcattataaa tatttaatgt taatttctta tttagataac
      301 aataaaaaaa tataatctct aaaaacccaa tgaaacagct gctccaaaca attagttctt
      361 ctaattgaaa atcaaataaa taagtatttc ttcttacagt accaccaagt aacacaatac
      421 ccgcgaatat ggtccatcct atcgatttat ttttaactgg tttgacaata tttaattttt
      481 cataatcatt cttagtgatt ttgacatatg ttttcggtat gagccagtta ataaatggaa
      541 agaagaagac aatccaatta cttgccaaat caatcattaa atattcacta tcgtatttga
      601 ttattcgata tttagggttt ttattaataa ctttagattc gcaaagcaat gtctccacat
      661 ccctttaatt ttatgtgtaa tacatttttc gatacttcaa aagacattca aatactatca
      721 agttactgtc atcaaaggtt ttattaact
//
LOCUS       DAKTGS010000274          740 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.283, whole genome shotgun sequence.
ACCESSION   DAKTGS010000274 DAKTGS010000000
VERSION     DAKTGS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.283"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..251
                     /locus_tag="O0286_002892"
     CDS             <1..251
                     /locus_tag="O0286_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0171177.1"
                     /translation="IFHNELPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDE
                     LDKTNYPKTELAVQEETGRTFELKLNHDEVKYICFKPS"
     gene            322..>740
                     /locus_tag="O0286_002893"
     CDS             322..>740
                     /locus_tag="O0286_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCY0171178.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKID"
BASE COUNT          277 a          113 c          121 g          229 t
ORIGIN      
        1 tcattttcca taatgaatta cctcaagact ctttgatgat tattaaaaca ttgaatcatg
       61 aacatggttc aattcaacat ttgcttccaa taagcgatca acttgtttat atagaaaaag
      121 aaattttaga tgaattagac aaaacgaatt accctaaaac ggagcttgca gttcaagaag
      181 aaactggtcg tacatttgaa ctcaagttaa atcacgacga ggttaaatat atttgcttta
      241 aaccaagcta aatactaaca gtcctcttgt gtttagtttc ttacgttaaa ggctatttat
      301 atcataagga gatgatttgt aatgagtaac tcacaagcaa ttcaagcaat tgaaaacgtg
      361 ttagcaacgt caaaagttgg tgtattatca actgcatata ataataaacc taatagtaga
      421 tatatggtct tttataatga tggtcttact ttatatacta aaacgaatat ccattctgct
      481 aaggtcaaag aaattaaaga taatccagca gcatatgttt tgttaggcta taacgacaca
      541 actaatcgta gtttcgttga gatggaagcg acaatcgaaa tcgttacaga acaagaagtg
      601 attgattggc tatgggaaac acaagacaaa agctttttca gttcaaaaga agatccagag
      661 ttatgtgttt taagagtagt tccgcaatcc attaagctaa tgaatgataa atcattagat
      721 acacctatca aaatcgattt
//
LOCUS       DAKTGS010000275          732 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.284, whole genome shotgun sequence.
ACCESSION   DAKTGS010000275 DAKTGS010000000
VERSION     DAKTGS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.284"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..145)
                     /locus_tag="O0286_002894"
     CDS             complement(<1..145)
                     /locus_tag="O0286_002894"
                     /inference="COORDINATES: protein motif:HMM:NF033859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMEK domain-containing protein"
                     /protein_id="HCY0171179.1"
                     /translation="MKRSTNQEKFLDTLIRLNTKIEELGKINILNNHIYSEYFFRDLL
                     NIVY"
BASE COUNT          229 a           93 c           91 g          319 t
ORIGIN      
        1 catatacaat atttaacagg tctctaaaaa aatattcgga atatatatga ttatttaaaa
       61 tatttatctt tcctagttct tcaatcttag tattaagtct aattagtgta tcaagaaatt
      121 tttcttgatt cgttgatcgt ttcaagtttt aagtctcctt ttaaaatgaa tatttcactt
      181 cttataacta atatttaaac aaaatgatca cataataata tttaatttac aatatgccac
      241 tacagtgtca acattttata aacactattc atttgaacat ggtctaaaac gaataggaca
      301 ttgtaaatga ttaaatagtc tgtctctagt tgatgtatat ggtcatttgt tatgcagata
      361 ataattaatc taattaattt ttacgtttta gttacttggt tgtgattgag gctttttgta
      421 tctagttgta atattctcct tttttagaat cataataggt acttcttgaa atttttagaa
      481 ctttgcacat tgctgatact gaatattgat acgcattttg ttgaaagact tcattttttg
      541 tctcaaaatt agcactgctt gctttaaatc atcattttac attatcaatc gatgtttttc
      601 tttgcgtaat tttattaact ctttttcatc acttgttaaa atgtcttgat gattgaatga
      661 ctcagtattt tggttttgtt ttatctaatt tgagaaagtt aatgatgtta gatcatattc
      721 tcgtataatt tc
//
LOCUS       DAKTGS010000276          718 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.285, whole genome shotgun sequence.
ACCESSION   DAKTGS010000276 DAKTGS010000000
VERSION     DAKTGS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.285"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..289)
                     /locus_tag="O0286_002895"
     CDS             complement(<1..289)
                     /locus_tag="O0286_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0171180.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGH"
BASE COUNT          256 a          128 c           70 g          264 t
ORIGIN      
        1 aatgtccgaa tacaaatctc ggtaaatatg caggttcatc aaaattaaaa tcagtttgtt
       61 gtttatacca cttccaaaat tcagtctcat catctaaatt taaactcatc tttttcgaag
      121 gcatattaat tagcagctca gaactatcat tttgaaatgg tacgccctgt cccatattta
      181 ctttatcatc gtataaatct atatctaatt gattaaactt cgggtgctta actaactctc
      241 tcaatacact tacaccagca gttcccatgc ctattattgc tacacgcata tatcatccat
      301 ccttttgtaa ttcaaaatat tatatttaaa ctattaccta taattatagc aatttggtat
      361 ttccaataaa atttattaag gtaagctatt atttcacttt cacaaccatt aaaccattta
      421 aaacttttca tttaatatca accttagtgt ttgtgttaaa atatttttaa cgaggtgaat
      481 taaattgaaa tttatagcaa agatattaat ctcattagta attctattta tgatttttag
      541 tcgtaaaaac aaagcttctt aatcaatacc cttgattaca aaataaattc aactaaggtg
      601 attggccata cactcaagat atggctaatc acctttttca atgcagaaca aagagtatat
      661 tttgcaaaat caatatctat aatttatctt taataccctt ttaatactaa aaccaaat
//
LOCUS       DAKTGS010000277          707 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.286, whole genome shotgun sequence.
ACCESSION   DAKTGS010000277 DAKTGS010000000
VERSION     DAKTGS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.286"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..355
                     /locus_tag="O0286_002896"
     CDS             <1..355
                     /locus_tag="O0286_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171181.1"
                     /translation="LELKYPEKEFEYGRLLVGQHKRDDLDVYYFGDTFLMCTIISFKT
                     FEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLMLTDFQKENYNKYI
                     QGQKQRVIFENGHFV"
BASE COUNT          254 a          101 c          119 g          233 t
ORIGIN      
        1 tttagaattg aaatatccag aaaaagagtt cgaatatggt cgtttgttag ttggacaaca
       61 taaacgtgat gatttagatg tttattactt tggtgatacg tttttaatgt gcacgattat
      121 ttcattcaag acatttgaaa ttaaagaaac agtagaatta tcatatgatg ctgttaatcg
      181 tattgtgtta aaagatggat ggttatttag aaaaatgaga atagaaacaa tgcaaaaagt
      241 gttaaaatac ggtacatcta aattaatgtt aactgatttt caaaaagaga attataataa
      301 atatattcaa ggtcagaaac aacgcgtgat atttgaaaat ggccattttg tctaattgat
      361 agtgaatata attagagtaa gaggctggga cataaatccc taaaaaacag cagtaagata
      421 attttcaatt agaaaatatc ttactgctgt tctctattta tacaatactt cgtattgaat
      481 ggcttcgctt tcctagggtg ccgtctcagc ctcggtcttc gactggcact gctccctcag
      541 gagtctcgcc attaatacta cgtattaaca tgtaatttta ctttgaaata ctttaaaaaa
      601 ataagacact ttgcccaact tacactacca ataaaaactt ctgttagaat tcctcaaaat
      661 gatatttcgc gacatgttaa tgaaattgtt gaaacgatac ctgatag
//
LOCUS       DAKTGS010000278          689 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.287, whole genome shotgun sequence.
ACCESSION   DAKTGS010000278 DAKTGS010000000
VERSION     DAKTGS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.287"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(282..515)
                     /locus_tag="O0286_002897"
     CDS             complement(282..515)
                     /locus_tag="O0286_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171182.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          241 a          103 c           67 g          278 t
ORIGIN      
        1 tcatatatat taatgatgaa gaagtccata caaaatccgc atcatttgtt gttaataatg
       61 ggactataat taatccgaaa ttaatcatgc atgctatata tactataatg ttatacacaa
      121 tgttaatttt tgttcaccac cttatacttc tattttaaaa acttctttat aatgatatat
      181 tgtttaatgt tgaaataatt agattatcta attttcattt gctttacatg taaaaggcta
      241 tatatagtat gctctttatg attctaaatg ctttttaata tttaatgctc atcaacattt
      301 ggattttgaa tattcaattc aaaaacttta ttagctacgt caattgtaaa atcagaacca
      361 tagttgacat gagctacttt taattttcca tctaaataat agattgcgat tgcaacatcg
      421 taaaattcgt caatgacaaa taaactcttt tcgtttgtta caacctcatg ctctcctgag
      481 tatacaacgt taatttccca atcattaaaa accatttgtt aacctccttg aacatttaaa
      541 ttgattcaac ttaagtttaa cttattcata caacttcgta caatatctag atgaacatta
      601 attgtatttc tagaaatctt tttcaattat atgtactaat tatactttta aatttcttat
      661 ttcagtatag ttttaaaacg attttaaaa
//
LOCUS       DAKTGS010000279          644 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.288, whole genome shotgun sequence.
ACCESSION   DAKTGS010000279 DAKTGS010000000
VERSION     DAKTGS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.288"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            422..504
                     /locus_tag="O0286_002898"
     tRNA            422..504
                     /locus_tag="O0286_002898"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:455..457,aa:Leu,seq:gag)
     gene            527..600
                     /locus_tag="O0286_002899"
     tRNA            527..600
                     /locus_tag="O0286_002899"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:559..561,aa:Gly,seq:tcc)
BASE COUNT          241 a           90 c          104 g          209 t
ORIGIN      
        1 taggatgaaa ttaaaatgat tagattaaaa atcttggcgt attttagtaa taattaatat
       61 ataaacttta cccaattaaa gctttaaagt agcacaaaag ataattatac atttttcatg
      121 acattttgat tgaacttttc tattgactgt acagcttata gttagtatga ttaaacacgt
      181 tccttaaaaa agaacaaatt aaataaactt tgatttgaaa gacagtcaat tctaaattta
      241 atttataaaa tatgttgaca ataagttcta aatgttttat tataaatagc gttgtgttaa
      301 caactgataa cacaaattaa tttaatgtaa aaaaacgatt aaaagttatt gacaaatatt
      361 tttaaacata atataataaa taacgttctg aaaaacgaag atgttttaaa tgcctatatc
      421 cgcggtcgtg gcggaatggc agacgcgcta ggttgagggc ctagtgggag aaatcccgtg
      481 gaggttcaag tcctctcggc cgcatcaaaa ttcttaattt aaataagcgg gtgtagttta
      541 atggcaaaac ctcagccttc caagctgatg ttgtgggttc gattcccatc acccgctcca
      601 tagataattt taatgaacat tgaaaactga atgacaatat gtca
//
LOCUS       DAKTGS010000280          637 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.289, whole genome shotgun sequence.
ACCESSION   DAKTGS010000280 DAKTGS010000000
VERSION     DAKTGS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.289"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            35..>637
                     /locus_tag="O0286_002900"
     CDS             35..>637
                     /locus_tag="O0286_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0171183.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIVISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITVVFALC
                     NLYYFAAVQNHYDLFNYWLMGSISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSSFIYIEKIIEYILVPNVVESKFIIIVSMLVIAG"
BASE COUNT          219 a           68 c          101 g          249 t
ORIGIN      
        1 atgctattta aaaaataatg tataggagat agatatgact actaaagaaa atattgatac
       61 tcttcgaaag ccaggcgcac aagccttaag tttaatatca ttgtttttga tacttttttc
      121 atgtttgact ttcttttttg gtttagatta tgaaaggttt ccaaattatt taaagataac
      181 gacaattata gaattaataa ttattgtaat tagtttactt caatggatta gatttataga
      241 tttcgaaaaa gaaagtgcac agaaatataa aaaaatatat gctcgatttt tagttgttat
      301 aaatgtgtta actactatca ctgtagtatt tgcactgtgt aatctttatt attttgcagc
      361 tgtacaaaac cattatgatt tatttaatta ttggttgatg ggttcgattt caatcataat
      421 tagctattta ttattagtaa ttggcggaat gttcacgtta ttaaaattac ctaaagtaac
      481 aaaacgttgg ggtggtaaaa ctaaaacaca tttcggttta ttattaactg cgttgagctc
      541 atttatatat attgaaaaaa ttatcgaata tatattggtt cctaatgtcg tagaatctaa
      601 gtttataatt attgtgagca tgttggttat cgctgga
//
LOCUS       DAKTGS010000281          607 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.290, whole genome shotgun sequence.
ACCESSION   DAKTGS010000281 DAKTGS010000000
VERSION     DAKTGS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.290"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            66..>607
                     /locus_tag="O0286_002901"
     CDS             66..>607
                     /locus_tag="O0286_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HCY0171184.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALK"
BASE COUNT          267 a           75 c           94 g          171 t
ORIGIN      
        1 tttaattaaa atatatagcg cactatgtta taatgtttgt atattattgt aaggagtgtt
       61 tcatcatgaa taaacaacaa agtaaagtac gctattcaat tagaaaagtt agtattggaa
      121 ttttgtcaat ttcaataggt atgtttttgg cattgggtat gtcgaacaaa gcatatgcag
      181 atgaaattga taaatctaaa gattttacaa gagggtatga gcaaaatgta ttcgcgaaat
      241 cagagttaaa tgctaataaa aatacgacaa aagacaaaat aaaaaatgaa ggtgctgtta
      301 aaacatcgga cacaagttta aagttagaca acaaatcagc aatttcaaac ggaaatgaaa
      361 ttaatcaaga tataaagatt tcaaatactc cgaaaaactc aagccaaggt aacaatctag
      421 ttattaataa caatgaactt actaaagaaa ttaaaattgc aaacttggaa gctcaaaatt
      481 ctaatcagaa gaaaacgaat aaagttacta ataattactt tggttactac agttttagag
      541 aagctccaaa aacacaaatc tatactgtaa aaaaaggaga cacacttagt gctatagcat
      601 taaaata
//
LOCUS       DAKTGS010000282          542 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.292, whole genome shotgun sequence.
ACCESSION   DAKTGS010000282 DAKTGS010000000
VERSION     DAKTGS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.292"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(31..103)
                     /locus_tag="O0286_002902"
     tRNA            complement(31..103)
                     /locus_tag="O0286_002902"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68..70),aa:Lys,seq:ttt)
     gene            complement(107..181)
                     /locus_tag="O0286_002903"
     tRNA            complement(107..181)
                     /locus_tag="O0286_002903"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:Gln,seq:ttg)
     gene            complement(191..274)
                     /locus_tag="O0286_002904"
     tRNA            complement(191..274)
                     /locus_tag="O0286_002904"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Tyr,seq:gta)
     gene            complement(304..379)
                     /locus_tag="O0286_002905"
     tRNA            complement(304..379)
                     /locus_tag="O0286_002905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(344..346),aa:Val,seq:tac)
     gene            complement(387..458)
                     /locus_tag="O0286_002906"
     tRNA            complement(387..458)
                     /locus_tag="O0286_002906"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(423..425),aa:Glu,seq:ttc)
     gene            complement(460..534)
                     /locus_tag="O0286_002907"
     tRNA            complement(460..534)
                     /locus_tag="O0286_002907"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(500..502),aa:Asn,seq:gtt)
BASE COUNT          145 a          140 c          133 g          124 t
ORIGIN      
        1 gcacttccca aaaatggaaa gtgcaagtag tgagccatag aggattcgaa cctctgaccc
       61 tctgattaaa agtcagatgc tctaccaact gagctaatgg ctctaatggc tgggctagct
      121 ggattcgaac caacgagtga cggagtcaaa gtccgttgcc ttaccgcttg gctatagccc
      181 aatatataga tggtggaggg gggcagattc gaactgccga acccgaagga gcggatttac
      241 agtccgccgc gtttagccac ttcgctaccc ctccagctta ttcatataat ttaataatca
      301 aaatggtgga gaatgacggg ttcgaaccgc cgaccctctg cttgtaaggc agatgctctc
      361 ccagctgagc taattctcca aaataatgac tcctacggga ctcgaacccg tgttaccgcc
      421 gtgaaagggc ggtgtcttaa ccgcttgacc aaggagccat ggctccacag gtaggactcg
      481 aacctacgac cgatcggtta acagccgata gctctaccac tgagctactg tggattaata
      541 tt
//
LOCUS       DAKTGS010000283          529 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.293, whole genome shotgun sequence.
ACCESSION   DAKTGS010000283 DAKTGS010000000
VERSION     DAKTGS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.293"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            41..247
                     /locus_tag="O0286_002908"
     CDS             41..247
                     /locus_tag="O0286_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171185.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          223 a           57 c           74 g          175 t
ORIGIN      
        1 gattattata attataaata gaaagagagt gtgattaata atgaatgaga ttgaaactat
       61 tataagtgaa atagaaaagt tattaactaa caatacacca tatagtattt caaaaaactc
      121 aggtgtacca cgtcaaacag ttactgattt aaaggtaggt aaaactaaaa taaaagaagc
      181 taaatttaaa acgataatca agttatatga atatcaaaga acattagaaa ataaaacaga
      241 atgttaacaa aaatattttg ttaggttagg tggacaagaa attaaatttc taatgatttc
      301 atggttgtct gtccacgttt tgtccacctc aataagataa tctcttagtt ttagaaattg
      361 tacatggtta aataatcaaa aatgcatatt atgaaattat cattgccatt aaatcttgaa
      421 tgatctttaa gcgatgatat aaaaggctga acacagtaaa taagttaact ttaatgcagt
      481 attctatact atacatattt tgtaataatg tttatgcaca taatttttc
//
LOCUS       DAKTGS010000284          524 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.295, whole genome shotgun sequence.
ACCESSION   DAKTGS010000284 DAKTGS010000000
VERSION     DAKTGS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.295"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(142..456)
                     /locus_tag="O0286_002909"
     CDS             complement(142..456)
                     /locus_tag="O0286_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171186.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(431..>524)
                     /locus_tag="O0286_002910"
     CDS             complement(431..>524)
                     /locus_tag="O0286_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171187.1"
                     /translation="FILDILHIVYNIDNNNFSGVKFNDWTNKFI"
BASE COUNT          180 a           80 c           68 g          196 t
ORIGIN      
        1 atattttttg aataaatcaa gtattaacta gtcacggaag gtagataaat aaatttgatc
       61 attctgaata ataacgaatg cattaaaaaa tgcagtgaca atctaacatt aattgttaaa
      121 atcactgcaa gcatcttgat tttatttagc tgtttgtgtc cagtcatgct tacctctgaa
      181 acgttttaca tgagcatata tttggtcagc agtataatct aaatttgtat aaactgtaat
      241 actgaacgct gattggtttt cagcaaatga ttcttcggaa aataattcct ttaagtgcat
      301 aaataaatca ttcatttgtt tatggtttaa accgggatat tcccttaaag ttctctttaa
      361 taattgtccg ttcttttctt ctaatgcttg aactacaata acaaatcttt catcttgctt
      421 tagtacatca tcaaataaat ttgtttgtcc aatcattgaa tttcacccct gaaaagttat
      481 tgttatctat attatacacg atatgcaaaa tatctaaaat aaag
//
LOCUS       DAKTGS010000285          506 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.296, whole genome shotgun sequence.
ACCESSION   DAKTGS010000285 DAKTGS010000000
VERSION     DAKTGS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.296"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..>506
                     /locus_tag="O0286_002911"
     CDS             <1..>506
                     /locus_tag="O0286_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0171188.1"
                     /translation="LVISITAGCGIGKEAEVKKSFEKTLSMYPIKNLEDLYDKEGYRD
                     DQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDEGK
                     PHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYGDFKNLKNYKDGDISYN
                     PEVPSYSA"
BASE COUNT          210 a           59 c           99 g          138 t
ORIGIN      
        1 ttttagtcat tagtatcact gctggttgtg gcataggtaa agaagcggaa gttaagaaaa
       61 gctttgaaaa aacattgagt atgtacccta ttaaaaatct agaggattta tacgataagg
      121 aaggctatcg tgatgatcag tttgataaaa atgataaagg tacatggatt ataaattctg
      181 aaatggttat tcaacctaat aatgaagata tggtagctaa aggcatggtt ctatatatga
      241 atagaaatac caaaacaaca aatggttact actatgtcga tgtgactaag gacgaggatg
      301 aaggaaaacc gcacgacaat gaaaaaagat atccggttaa aatggtcgat aataaaatca
      361 ttccaacaaa agaaattaaa gatgaaaaaa taaaaaaaga aatcgaaaac tttaagttct
      421 ttgttcaata tggcgacttt aaaaatttga aaaattataa agacggagat atttcatata
      481 atccagaggt gccgagttat tcggct
//
LOCUS       DAKTGS010000286          500 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.298, whole genome shotgun sequence.
ACCESSION   DAKTGS010000286 DAKTGS010000000
VERSION     DAKTGS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.298"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..>500
                     /locus_tag="O0286_002912"
     CDS             <1..>500
                     /locus_tag="O0286_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCY0171189.1"
                     /translation="HDINNLIDYDFGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNY
                     RRNSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKE
                     INAIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKLSNSSTTENISIDISRFLELSN
                     MILLLD"
BASE COUNT          207 a           60 c           73 g          160 t
ORIGIN      
        1 catgatataa ataatttgat tgattatgac ttcggaattg attttgcaga aagagcaatc
       61 aaaaatgaag acatagttaa taaaaatgtt aatttttttc aacaaaacag gcttaaagag
      121 attgttaatt atagaaggaa tagtgtagat tacgttagac cttcagaatc ttatatatca
      181 gtccaaggac atccacagaa tcctcaaatt tttggaaaaa caatgacttg tggtacaagt
      241 atttcattgc gtgtaccgaa tagaaagcag caattcatag ataaaattag tgtgataatc
      301 aaagaaataa acgctattat taatcttcct caaaaaatta gtgaatttcc tagaatagta
      361 actttaaaag acttgaataa aatagaagta ttagatactt tattgctaaa aaaactatcg
      421 aattcttcaa ctacagaaaa tatatctata gatatatcaa gatttttaga actaagtaat
      481 atgatactct tgttagatga
//
LOCUS       DAKTGS010000287          499 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.299, whole genome shotgun sequence.
ACCESSION   DAKTGS010000287 DAKTGS010000000
VERSION     DAKTGS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.299"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            78..>499
                     /locus_tag="O0286_002913"
     CDS             78..>499
                     /locus_tag="O0286_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0171190.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINR
                     IRVNPNTDALIESLFIQFHSQCLKQNLIDGTKVEANAN"
BASE COUNT          180 a           80 c           70 g          169 t
ORIGIN      
        1 aataatttga attttctgtg ttgggtccct tcgtataatt taataaatac cactaaacta
       61 aattaatgaa gtgccttatg tataaaaatt ataacttgat ccaacttaca ctaccaatag
      121 aaacttctgt tagaattcct caaattgata tttcgcgata tgttaatgaa attgttgaaa
      181 cgatacctga tagcgaattc gataaattca gacatcatcg tggcgcaaca tcctatcatc
      241 caaaaatgat gttaaaaatc accttatatg catatactca atctgtattt tctggtcgta
      301 gaatagagaa attacttcat gacagtattc gaatgatgtg gttagctcaa aatcaaacac
      361 cttcttataa aactattaat cgtattagag tgaatcctaa tactgatgcg ttaattgaat
      421 ctttatttat ccagtttcat agtcaatgtt taaagcaaaa tcttattgat ggtacaaaag
      481 tagaagctaa tgccaatag
//
LOCUS       DAKTGS010000288          498 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.300, whole genome shotgun sequence.
ACCESSION   DAKTGS010000288 DAKTGS010000000
VERSION     DAKTGS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.300"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..>498
                     /locus_tag="O0286_002914"
     CDS             <1..>498
                     /locus_tag="O0286_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0171191.1"
                     /translation="FIKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYL
                     SGRTIYINNNHSINNTRFSIARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQF
                     SVDLLMPKKQIEALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLY
                     NLGIH"
BASE COUNT          192 a           60 c           79 g          167 t
ORIGIN      
        1 catttataaa ggacaaaatg gatcaacttt ttattacttc tgaacacttt gctattgaca
       61 tagacaagct attatttcaa cttaatttaa atgttagata tattaaaatg gagggtaata
      121 cttcgggtta tttaagtgga agaacaatct atattaataa taatcactcg attaataata
      181 caagattttc aatagctcgt gaattgggcc gttatttatt taaaaataca gacttaatga
      241 gagatagtaa cgataatagt ctaaagaatt tacatgaaat gatttatgaa agcgacgtaa
      301 atcagttttc agtcgactta cttatgccta aaaagcaaat agaggcatta gtctataatt
      361 tttatgaagt caataatatc aatttaagtt ctggtttatc tgaaaaagaa cgaaacaaac
      421 tattgaacct tatatcaact aagttggaag tttcgaaagt agctgctgga ttaaggctgt
      481 ataatttagg aatacata
//
LOCUS       DAKTGS010000289          498 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.301, whole genome shotgun sequence.
ACCESSION   DAKTGS010000289 DAKTGS010000000
VERSION     DAKTGS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.301"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
BASE COUNT          191 a           57 c           63 g          187 t
ORIGIN      
        1 gttatttttt aataatttac accaaattaa tagcaaaaat tatgttattc gtgctaatat
       61 ttcatagttg gttattcaat taattaaaaa taagtcaaaa tgcacaactt tttataattc
      121 attgagtcga gtttgaaaaa taaaagtgct ttaatgcatg atcaattatc gtactttcta
      181 ttatttgtta cccgttatca atcggaataa cgtatagaca ctttaacgtg ctatagattg
      241 gttttaatca ctaaattaat gtgtttttct tatcattaaa actgcactga gaattactaa
      301 attaaaaaaa ttataaaaat ttttcatttt tagtgataaa attctgaaaa atgggtataa
      361 atagtagaag aagttaactt ggaagagtta agctataaca aagaatctct ttagacacac
      421 attgaatatc gaaacattta attgcgctaa atcgtttcat taaataaatt accttgtatt
      481 gtcgattaaa ttaaggta
//
LOCUS       DAKTGS010000290          479 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.302, whole genome shotgun sequence.
ACCESSION   DAKTGS010000290 DAKTGS010000000
VERSION     DAKTGS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.302"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(180..479)
                     /locus_tag="O0286_002915"
                     /pseudo
     CDS             complement(180..479)
                     /locus_tag="O0286_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          151 a           81 c           51 g          196 t
ORIGIN      
        1 ccttaaattt aacaaattaa atcaaaccga atgaggctaa attggttatt tttcaagata
       61 tgatttgtat aaatcaaaac atttcttaca aatcaaatgt gtataaaatg tgatatacat
      121 tttcaacctt aaaaaagttc taaaaaaaga tgagcatcta ctgtctcatc tttcagttct
      181 tttttacggg tctgttttct aatttgagca caatcttcga tttctttatc taaatgacta
      241 ccaattaaat ctatttcttc tattgttaaa tcgctatctc catcttcttt tatctctggt
      301 attatttttt cttcaactaa gtcacgatat agtgtttttg aattctcgtt caatttcgat
      361 tcgtgatttt gaatactttc ttccgcacca atgtatatct attggcacta gcttctactt
      421 ttgtaccatc aataaaaatt gaattatcat caataagatt ttgctttaaa cattgacta
//
LOCUS       DAKTGS010000291          460 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.303, whole genome shotgun sequence.
ACCESSION   DAKTGS010000291 DAKTGS010000000
VERSION     DAKTGS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.303"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            313..405
                     /locus_tag="O0286_002916"
     CDS             313..405
                     /locus_tag="O0286_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171192.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
BASE COUNT          180 a           73 c           44 g          163 t
ORIGIN      
        1 taaatcacgt gcatttacgc tcactagctt tcatctcctc atttttgctt ttcaaattta
       61 ttaagtatga ataaaaaata catccgaaca aacaaaatct ctatattcta attcgattta
      121 catagattat acactaaaaa aatgattctc aatgcaaatg aatgtgtctc tcatagtcta
      181 aaaactcgta tgtttcgaat tttatgattc attgataata gtaccagtta aaagaactaa
      241 gattgtaatt atcccccctt aaaattcaag aatatatttt cttaatctat cagcaagagg
      301 ttatatttca aagtgtttat ttttaaaaca acatctaaaa gccattttca taataatgtt
      361 aaatcattag aatgtataaa gattccaatt aataaaaata gataaaatgc aataaagttc
      421 caaatactta ttattattca tcacaaatta cgtgatgccc
//
LOCUS       DAKTGS010000292          457 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.304, whole genome shotgun sequence.
ACCESSION   DAKTGS010000292 DAKTGS010000000
VERSION     DAKTGS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.304"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..79
                     /locus_tag="O0286_002917"
     CDS             <1..79
                     /locus_tag="O0286_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0171193.1"
                     /translation="YIMMTANDDVEAPSDYEKIRAEVSW"
     gene            147..347
                     /locus_tag="O0286_002918"
     CDS             147..347
                     /locus_tag="O0286_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0171194.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
BASE COUNT          159 a           44 c           92 g          162 t
ORIGIN      
        1 gtatattatg atgacggcta atgatgatgt agaggcgccg agtgattacg aaaaaatcag
       61 agctgaagtt tcatggtaat agctattatc atttttgaat taattatatt aatgtgttta
      121 gcaatagcac tggaggtgtt gtaaatatgt ggattgtcat ttcaatcgtt ttagctatat
      181 ttttattgat cttgttaagt agcatttctc ataagatgaa aaccatagaa gcattggagt
      241 atatgaattc ttatcttttc aagcagttag taaaaaataa tggtgttgaa ggtttagaag
      301 attatgaaaa tgaagttgaa cgaattagaa aaagattcaa aagctaaaga gggggctaaa
      361 gccctctcca ggattaaaag aagcggatat tttttatttc gtttttgtaa attaaaacta
      421 atttatgtga aattgtcttt gaagtcttga taattaa
//
LOCUS       DAKTGS010000293          399 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.306, whole genome shotgun sequence.
ACCESSION   DAKTGS010000293 DAKTGS010000000
VERSION     DAKTGS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.306"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..>399
                     /locus_tag="O0286_002919"
     CDS             <1..>399
                     /locus_tag="O0286_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171195.1"
                     /translation="KYSDNLIKVFSEDVWNIMGYKKYNEYFTNRDDTTLKITNLYIGQ
                     SFGTEGNRNVIDRIGKGHEKLQEIMTYQPENRKTVLIFYKIQPDNIYITNQYGKEAVS
                     RLKRILEQSIPNKEYIDLSEISLITFFKPEY"
BASE COUNT          171 a           40 c           63 g          125 t
ORIGIN      
        1 aaatatagtg ataatttgat aaaagtattc tctgaagacg tttggaacat aatggggtat
       61 aaaaaatata atgaatattt tacaaacaga gatgatacta cattaaaaat tactaattta
      121 tatattgggc aatcttttgg tacagagggt aatagaaatg tcattgatag aatagggaaa
      181 ggtcatgaaa aattacaaga aattatgact tatcagccag aaaatagaaa gactgtctta
      241 atattttaca agatacagcc tgacaatatt tatattacaa accaatatgg taaagaggct
      301 gtgtctcgat taaaaagaat tttagagcaa agtataccta ataaagaata tattgattta
      361 tcagagatat cattaattac attttttaaa ccagaatat
//
LOCUS       DAKTGS010000294          386 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.307, whole genome shotgun sequence.
ACCESSION   DAKTGS010000294 DAKTGS010000000
VERSION     DAKTGS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.307"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
BASE COUNT          119 a           64 c           61 g          142 t
ORIGIN      
        1 taacgattat caatgtcgcg tggatttgtg acacatttct tttaaaaatt cacaaggtta
       61 tggggcagaa atgataaaga gccactaatg atttattatg tagtggttct gggagtggga
      121 cagaaatgat attttcacaa aatttatttc gtcgtcccac cccaacttgc attgtctcta
      181 gaaattggga atccaatttc tctttgttgg gtccctgaat atagccttgt agagtctagt
      241 acattgattt gtatcccaat gtccctataa ttgattattc gctttatcta atgatcctat
      301 gactcaacta ttaaatcatt tttcgaaata cttaattcta atataattaa attcatttat
      361 tgtaatattg caaaaataca ttgcac
//
LOCUS       DAKTGS010000295          361 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.308, whole genome shotgun sequence.
ACCESSION   DAKTGS010000295 DAKTGS010000000
VERSION     DAKTGS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.308"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(45..>361)
                     /locus_tag="O0286_002920"
     CDS             complement(45..>361)
                     /locus_tag="O0286_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0171196.1"
                     /translation="IPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTSCEFD
                     FTREGELKVSFDYIDWINSEFGQIGRQNYYKYRKFGILPETEYEINKVKEIEQYIKEL
                     EE"
BASE COUNT          121 a           65 c           29 g          146 t
ORIGIN      
        1 aagactaaac aaagctttat tgtttagtct tacattaaga tagtttattc ttctagctct
       61 ttaatatatt gctcgatttc tttaacttta ttaatttcat attccgtttc tggtaaaatt
      121 ccaaattttc tatacttata gtaattttgt cgacctattt gaccaaattc tgaatttatc
      181 caatcaatat aatcaaatga aacttttaat tcaccttctc ttgtaaaatc aaattcgcat
      241 gatgtccatg gttctaaatc ttcttcttta aataaatctc ttaactcctc aaataaatca
      301 tataaatcca tccataaatc atcaaatact tgcacagaaa tgttatactc cttaggtata
      361 t
//
LOCUS       DAKTGS010000296          344 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.309, whole genome shotgun sequence.
ACCESSION   DAKTGS010000296 DAKTGS010000000
VERSION     DAKTGS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.309"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..>344
                     /locus_tag="O0286_002921"
     CDS             <1..>344
                     /locus_tag="O0286_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171197.1"
                     /translation="FDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPVTFANA
                     YLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPKVDLST
                     TVVILTIIGAIL"
BASE COUNT          113 a           40 c           60 g          131 t
ORIGIN      
        1 catttgataa aaaagttaaa aagcaattat ggtttttgaa caagaaggag aaacaagcat
       61 tagatcaacg attgtcttct atatctgatg atgacagcgt taacttaaat aagcctgtta
      121 cctttgctaa tgcttactta agacaaaatg tatttcgaaa taaagaaaca aaaagttata
      181 gtatgtttgt tacattagta gtgatgatgt ttgcttatgt tgctttatta ggtttgtttt
      241 tatttgggct aattacaagt ctatctggcg tacaattttt cgttagtcct aaggtagatt
      301 tatctacaac tgttgttatt ttaacaatta ttggagcgat ttta
//
LOCUS       DAKTGS010000297          324 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.310, whole genome shotgun sequence.
ACCESSION   DAKTGS010000297 DAKTGS010000000
VERSION     DAKTGS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.310"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..>324)
                     /gene="deoC"
                     /locus_tag="O0286_002922"
     CDS             complement(<1..>324)
                     /gene="deoC"
                     /locus_tag="O0286_002922"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0171198.1"
                     /translation="YIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVKYAAER
                     LADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRFDDVQQ
                     DIEAVV"
BASE COUNT           82 a           71 c           57 g          114 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaattg catcttctgt ttcaaatgct
      121 ttcgttgcag ttgtcgacgc acctaatggg aatcctatta ccgtacaaac gagcacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgtgttg gattcacaca tacagattta
      241 aaattgtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatctat atat
//
LOCUS       DAKTGS010000298          322 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.311, whole genome shotgun sequence.
ACCESSION   DAKTGS010000298 DAKTGS010000000
VERSION     DAKTGS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.311"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..267
                     /locus_tag="O0286_002923"
     CDS             <1..267
                     /locus_tag="O0286_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0171199.1"
                     /translation="KKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFKSIV
                     NTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            251..>322
                     /locus_tag="O0286_002924"
     CDS             251..>322
                     /locus_tag="O0286_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCY0171200.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPV"
BASE COUNT          132 a           34 c           48 g          108 t
ORIGIN      
        1 aaaaaaaata taaatatgta tcaatattta gttcctagtg atttaggatt gaaaaattca
       61 caagttgttc agtttccaat aagtaatatt tctagtaaga aaatcttgca caataaattt
      121 aaatcaatag ttaacactga agaaattgat gaaaatattt ctttctataa acttgtgaac
      181 aagttttcac ctgaagtagc acctgaagat acagtggatt tacttcagat aataaaaagg
      241 aggagtaaag atggaaattt taaatgattc tgtaaaaagt tttgaaaaac tttatttaca
      301 attacaagat ggttttccag tg
//
LOCUS       DAKTGS010000299          319 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.312, whole genome shotgun sequence.
ACCESSION   DAKTGS010000299 DAKTGS010000000
VERSION     DAKTGS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.312"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(<1..184)
                     /locus_tag="O0286_002925"
     CDS             complement(<1..184)
                     /locus_tag="O0286_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCY0171201.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSL"
BASE COUNT          100 a           44 c           43 g          132 t
ORIGIN      
        1 ttaatgattg tattaaaatt aaacattcat tcatatttcc ttgtactgtc gtttctaacg
       61 gacctactct aaaatgcaaa ccagattcgt caattatttt aatcgcctcg tctacgtaag
      121 gtataacatt gtcattgttt ggtgttttag gaattatttg tatactcatt aatgtgtctt
      181 tcatttttaa aaactccttt attttttgta atagtaaaaa actgagtagc ataaatggat
      241 ctgttataaa tacatttata cgactcagtt ttaattatta aataaattgt tcagttaatg
      301 cattgaaggt gtcttcagt
//
LOCUS       DAKTGS010000300          306 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.314, whole genome shotgun sequence.
ACCESSION   DAKTGS010000300 DAKTGS010000000
VERSION     DAKTGS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.314"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(1..236)
                     /locus_tag="O0286_002926"
                     /pseudo
     CDS             complement(1..236)
                     /locus_tag="O0286_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           93 a           49 c           41 g          123 t
ORIGIN      
        1 gtttctggtt cagaaagctt ttgattaatt tgggctttaa agtattccca attataattc
       61 ttcataattt tcttattgga tttcgaattt ggcttcatgc attgatgtct caaagaacat
      121 gatgaacagt catcacattc atatagcttg aagtcacgtt taaaactata tctatcatta
      181 cggtatgcat atcttttaaa acctattctt ttgttattag gacatataaa ttcatcgaat
      241 tcgctatcag atatcgtttc aacaatttca ttaacatatc gcgtattatc attttgagga
      301 attcta
//
LOCUS       DAKTGS010000301          288 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.315, whole genome shotgun sequence.
ACCESSION   DAKTGS010000301 DAKTGS010000000
VERSION     DAKTGS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.315"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
BASE COUNT          129 a           47 c           39 g           73 t
ORIGIN      
        1 accttattgt atttatagaa aaacaaaaaa aggtaagcac cgaaatgctt acctacttcc
       61 cataaacaat ataacacata tacattgatt tggaaagcgc aaaaataaat ttaatatact
      121 agcccgaagg ggagcaatac ataaaaaatg aaaggcgctc cttgaaaacg cccaaggtaa
      181 tattaacata aaatggctac tattgcatta tctaatttta tttaattaaa acaaatatat
      241 atagcacaaa aaaactagcc cgaaggctag cttatgaata gatgaaaa
//
LOCUS       DAKTGS010000302          278 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.316, whole genome shotgun sequence.
ACCESSION   DAKTGS010000302 DAKTGS010000000
VERSION     DAKTGS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.316"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(24..>278)
                     /locus_tag="O0286_002927"
     rRNA            complement(24..>278)
                     /locus_tag="O0286_002927"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           76 c           63 g           70 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaact
//
LOCUS       DAKTGS010000303          272 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.denovo.318, whole genome shotgun sequence.
ACCESSION   DAKTGS010000303 DAKTGS010000000
VERSION     DAKTGS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.denovo.318"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            complement(1..272)
                     /locus_tag="O0286_002928"
                     /pseudo
     CDS             complement(1..272)
                     /locus_tag="O0286_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
BASE COUNT          100 a           47 c           31 g           94 t
ORIGIN      
        1 cagtgattgc tagttgtttt tcaaactgaa cattaaagaa gttaccgtac tttctcaata
       61 taattccaaa tagaactgaa cttcctgcaa ccggccaaaa aacattattt ttaacaggct
      121 cgactatatt taacctttaa aattcatttt cgctaatttt tgcgtatttt ttgggcaaaa
      181 accaattaat catagggaaa aaataagtaa tccaagtact tactaaatcg accatgaaat
      241 attcattatt atatctaata actcgatatt ta
//
LOCUS       DAKTGS010000304         6869 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.142, whole genome shotgun sequence.
ACCESSION   DAKTGS010000304 DAKTGS010000000
VERSION     DAKTGS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.142"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..83
                     /locus_tag="O0286_002929"
     CDS             <1..83
                     /locus_tag="O0286_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0171202.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(255..1226)
                     /locus_tag="O0286_002930"
     CDS             complement(255..1226)
                     /locus_tag="O0286_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0171203.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            1350..1769
                     /locus_tag="O0286_002931"
     CDS             1350..1769
                     /locus_tag="O0286_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0171204.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O0286_002932"
     CDS             complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O0286_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0171205.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            3247..5004
                     /gene="blaR1"
                     /locus_tag="O0286_002933"
     CDS             3247..5004
                     /gene="blaR1"
                     /locus_tag="O0286_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCY0171206.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4994..5374
                     /gene="blaI"
                     /locus_tag="O0286_002934"
     CDS             4994..5374
                     /gene="blaI"
                     /locus_tag="O0286_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0171207.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            5638..6216
                     /locus_tag="O0286_002935"
     CDS             5638..6216
                     /locus_tag="O0286_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0171208.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(6439..6624)
                     /locus_tag="O0286_002936"
     CDS             complement(6439..6624)
                     /locus_tag="O0286_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171209.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
BASE COUNT         2574 a          950 c          945 g         2400 t
ORIGIN      
        1 ttttcgttca catcatagca ccagtcatca gtggctgtgc tgttgcgtat tatacttatt
       61 ggcttagtaa acgcaacaaa taattaatag gcgactttat aagccagcat cgaaaaaatc
      121 cccacactac tgcaatagtg tggggattgg tgcattaatt gttaatcatt ttcgttatat
      181 aaattataac actggttagt agagaaatgc aaaatgattg aattaatagt aaatgtatta
      241 atgaatactc acacttatac ttttaaaata gttcttccta aatgttgtcc ttttaaaagc
      301 tgatctatcg ttttatagaa ttcttcaaat ccaatttcat tgtatagaag tgaatttcct
      361 atattccatt catttgctaa cttactccat atatcttcac ggatattatg atcaacatat
      421 acagaatcaa tgccaattaa tttaatacct ctaagaataa acggtaatat attagcgtta
      481 agtgaatttc ctgctacatt tccacataca gtagcaacac cttgataatt tagacgtttt
      541 aaaccgtata atgttacatt actccccact gtatctaaga aataatcaaa aatacctttt
      601 ttaagcggtt tattatcatc ttcaattatt attacttgtt ttgcacctaa tttttttgcg
      661 atttctattt gcttttcttt tcttacgata gcagtgatat tttcatatcc aattttatgt
      721 aaaatttgta tagcaacact gcccacacca cctgtagccc cagacactaa aatttcaggt
      781 tgtgatttga tattcattcc atatttttct agttcaaaaa ttgataatgc tgccgtaaaa
      841 ccggctgtgc catataccat agactcttta atagacattt cttggggaat aggaacaatc
      901 cactctccag gcacttgtgc atattctgaa aatccacctg tatgtgtcac gccaacatca
      961 taaccagtaa ctaatacatt atcgccttct ttaaacttat cattcgaact tgattcaaca
     1021 atacctgcaa aatctatacc aggaatcata ggataatctc taattacacc gcctttatat
     1081 tgtaaagcta gcatatcctt ataatttatt gaagagtata ccacttttac taatacatca
     1141 cccttcgata aatgagattc atcaactttt tctactttat aactaacatt tgatgcactt
     1201 tttctcacta ctaccgcatt gaacatttat tagccttctt tcacaattgt tttgtagaag
     1261 tatgtttttg ttgcaaaact attttcctac aaaacttatc atgatatgat aagcgagtca
     1321 atactaatga tttaagagag gttttagttg tggaattaga taattgtata aattatttgt
     1381 tgagtacttc tcagaataga gtttttaaac attttaaaag tttattaagt ccctataata
     1441 ttactccagc agaatatggt gttttgaatt gcttgattaa tagtaatttg aatacaccta
     1501 aagcgattgg taatacttta aatttagaac cttcaacaat atctggaata ttagataaga
     1561 tgtccaaaaa ggatctaata acacgtcaaa ctgataatga aaatagaaga actgtgctaa
     1621 taaacagtac aaataaagca aaagacttat ggccaacatt agaaaagaaa gcctatcaac
     1681 ttaataatca ttacttcgct aatttaacta atgaagaaac tgtagtgttt aaaaagatat
     1741 tactaaagat aaataaaact acgttttaat ttttacgtta taaaaaagtt gaagtgcgaa
     1801 ttataatcga gtaaatataa atgatagctt agcttaatta tatattaaag ccaactatac
     1861 ttttattccc gaaaaaccta ctatccaaat ttctaagata ctttagcctc tataataaac
     1921 atattgtaat ataatttatg tattatttag gtttacccta atcgaacaat aaaaaatact
     1981 tgtaaaatgc tattgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga
     2041 aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt
     2101 aaactattca tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga
     2161 gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt
     2221 atacatgtca acgataatac aaaatataat acaaactata agatgttatc agtatttatt
     2281 atgcatttag aatattaaaa ttccttcatt acactcttgg cggtttcact tatcaactta
     2341 tcatttggct tatcactttt attgtcttta ttcgtaaaaa tgactaaaac aataggttca
     2401 gattggccct taggataaac aaaagcaaca tcatttctag aagcatatgt tattgcttga
     2461 ccacttttat cagcaacctt atagtctttt ggaacaccat ctttaattaa agtgtctccg
     2521 tttttattat taaacattaa atcaagtaag aaatttttat tttttttgct taattttcca
     2581 tttgcgataa gtttatttaa agtcttgccg aaagcagcag gcgttgaagt atcttttttg
     2641 ctctttggtg aatagtaatt taattctatc tcatatctaa ctggatttgt tactttatct
     2701 cctagttctt ttagacgttg tttaactttt ttgattccac cgatttcttt tataatttta
     2761 ttgtttgctg tattatcact atatgtcatt gaagcctcaa taagttcttt taaagtgata
     2821 tcttttccta catatttttc taaaatagga gaataagcaa ctatatcatc tttgttaata
     2881 tgtacttttt tatttaactt attataaggt acttgttcta acaaaatagc actatttatc
     2941 gcttttgaag tcgaagcata ggcaaatctc ttatctgaat taaattttac ttccttacca
     3001 cttttagtat ttaaagcata aacaccaata tgagcattat attttttttc taaatcattt
     3061 aactctttgg catgtgaact gtttgaatta catgcactta aaactaaagc aattacaatt
     3121 aaaaatatta actttttcaa aataaaccct ccgatattac agttgtaatt tttataaatc
     3181 aataatatta caattgtaat attgatgtca attgtttatt ataaatattt gaaagaaggt
     3241 gtcaaaatgg ctaaattact aataatgagc gtagtaagct tttgttttat atttctattg
     3301 ttagtatttt ttaggtacat attaaaacgc tattttaatt atagtttaaa ttataaagtt
     3361 tggtatctaa ctatgcttgc tggattaatt ccttttatcc caattaaatt ctcttttata
     3421 aaatttaata atgtgaataa tcaagcgccc acagttgaaa gtaagtcaca cgatttgaac
     3481 cataacataa ataccaccaa acctattcaa gagttcacaa cagatatcca taagtttaat
     3541 tgggattcaa ttgataatat ctgcacagtt atttggatag ttttagttat tattttaagt
     3601 tttaaatttt tgaaagcctt attatatctt aaatatttaa agaaacagtc actttatcta
     3661 aacgaaaatg aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaaat
     3721 attgtgattc gaaaagcaga ggctattcaa tctccaataa ctttttggta tgggaaatat
     3781 attattttga ttcctagttc atattttaaa agtgtaattg ataaaagact aaaatatata
     3841 atactgcatg aatatgctca tgctaaaaat agagatactt tacatttaat tatctttaat
     3901 atcttctcta ttattatgag ttacaatcca ttagtacata ttgtaaaaag aaagataatt
     3961 catgacaatg aagtagaagc cgatagattt gtccttaata atattaacaa aaatgaattt
     4021 aaaacttatg cggaatctat tatggactcc gtattaaaaa ccccattttc caacaaaaat
     4081 atattaagcc attcatttaa tggtaaaaaa tcattactca aaagtagatt aattaatata
     4141 aaagaagccg atctgaaaaa gcagtcaaaa ttaattctaa tttttatttg tatttttact
     4201 ttttttatca tgataattca aagccagttt ttaatggggc aatccttaac tgattataat
     4261 tataaaaagc ccttacaaag cgattaccaa attttagatg aaagtaaaaa ttttggttca
     4321 aatagtggat ctttcgttat gtacagcatg aagaaagaca aatactatat ttataatgaa
     4381 aaagaaagta gaaaaagata ttctcctgat tcaacttaca aaatttattt agcactgttt
     4441 ggacttgacc gtcatattat aagtgataaa aattcacgta tgtcatggaa tcataaacat
     4501 tatcttttcg agtcttggaa taaggaacaa gatttaaata cagcaatgca aaattcagtt
     4561 aattggtact tcgaacgtat tagtaatcaa ataccaaaga actatactgc ggctcaactc
     4621 aagcaattaa attatggtaa tgaaaatttg ggaagttata aaagctattg gatggaagat
     4681 agtttgaaaa tatctaatct tgaacaagta atagttttta aaaatatgat ggaacaaaat
     4741 aaccatttta gtaaaaaagc aaagaatcaa ttatcttctt cattattgat taagaaaaat
     4801 gaaaagtatg aactgtatgg gaaaacaggt acaggtatag taaacgggaa gtataataat
     4861 gggtggtttg taggttacgt aattacaaat catgataagt attattttgc tacacattta
     4921 tcagatggaa acccgtctgg gaaaaatgct gaattaataa gtgaaaaaat attaaaagga
     4981 atgggtgttt taaatgacca ataagcaagt tgaaatatct atggctgaat gggatgttat
     5041 gaatataata tgggataaaa aatcagtatc agctaatgaa attgtagttg aaattcaaaa
     5101 atataaagaa gttagtgata aaacgattag aacattaatc acaagactat ataaaaaaga
     5161 gattataaaa cgatacaaat cagaaaatat ttatttttac tcatcaaata ttaaagaaga
     5221 cgatattaaa atgaaaactg ctaaaacctt tcttaataaa ctgtatggag gggatatgaa
     5281 aagtttagtg ctgaattttg cgaaaaatga agaattaaat aacaaagaaa ttgaagaatt
     5341 gcgagacatt ttaaatgata ttagtaaaaa gtaattagag gtatgtttca aacataatac
     5401 atatgtaaac catacttgac tcttattaaa ttagttttgt taatgacatt taaaatatat
     5461 tattaaagat gagagtgtcc agaaaataac cattttctgt acactcttgt tttttgtcta
     5521 aataagtaag tattacatag aattatgtgt tcaaaaaccc aacaaaaaat ctatgttcac
     5581 aaaagacctc gtaatcaagt tatggtacac taaaggtgta tataggaggt ctgtattttg
     5641 aaaataggtt atgcaagagt ttcaactgga ttacaaaatt tgaatttaca ggaggatcga
     5701 ttaaatacat atggttgtga aaagatattt aatgaccata tgagtggttc aaaaagtaag
     5761 cgacctggtt tagataaagc aattgaattt gcgaggtcag gagatacgat tgttgtttgg
     5821 agattagata gactaggacg gaacatggag gatttaatca cgttagttaa tgaacttaac
     5881 gaacgaggtg tgagttttca tagtttagaa gaaaatataa cgatggataa atcaagttct
     5941 acaggacaat tattatttca tttattcgca gcatttgcag agtttgaacg taatttaatt
     6001 ttagagcgat catcagccgg tcgaattgca gcacgtgcta gaggccgata tggaggtaga
     6061 cctgaaaaat taaataaaca agatttaaac ttacttaaga cactttatga taatggtacg
     6121 ccaattaaaa caattgcaga gcagtggcaa gtttcacgta caactattta tcgttatctc
     6181 aataaattag aggcaaaaga gaataaaaac aagtagagca actaatctag cttactataa
     6241 tgaatctact aaataatttt atatatcatt aacatacaaa gactttgatt caatgtcact
     6301 cgatgtaaaa gagacgttct gacagcgtct aaaatctcat aatattttcc tgttatgaga
     6361 ttttaataat aacacaagaa aaaagcgtac aaattcgtac gcttttttaa tataatacct
     6421 gtttcactaa tgaacgtttt aagtatcttc atcattaact gaaactacag taataatact
     6481 cgtcaacaat attagtatag ttaaaataga aagacttagg actggcataa ccgaactaaa
     6541 attatatttg aaatataaaa gtgctaatga agagagaaat gtaaaaataa taaataccaa
     6601 agaccatttt tctaatttat tcattggctt cacctctttg aacttttata taatcatagg
     6661 agatattttt actacgattt ataaaaaata agaagataat cattattaaa gaaacgatta
     6721 atataatgtt gaatcctata gtatcaccta attcgtttac acctttgcct aatatcggag
     6781 atacaaagct ttgtattaat aatgaaagag atgcccatat agtaaatgag gttctgttgc
     6841 aaagttgaat ttatagtata attttaaca
//
LOCUS       DAKTGS010000305         6633 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.148, whole genome shotgun sequence.
ACCESSION   DAKTGS010000305 DAKTGS010000000
VERSION     DAKTGS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.148"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            458..1924
                     /gene="msr(A)"
                     /locus_tag="O0286_002937"
     CDS             458..1924
                     /gene="msr(A)"
                     /locus_tag="O0286_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0171210.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2023..2922
                     /locus_tag="O0286_002938"
     CDS             2023..2922
                     /locus_tag="O0286_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0171211.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3408..4016
                     /locus_tag="O0286_002939"
     CDS             3408..4016
                     /locus_tag="O0286_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0171212.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4262..4600
                     /locus_tag="O0286_002940"
     CDS             4262..4600
                     /locus_tag="O0286_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171213.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4605..4790
                     /locus_tag="O0286_002941"
     CDS             4605..4790
                     /locus_tag="O0286_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0171214.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5017..5760)
                     /locus_tag="O0286_002942"
     CDS             complement(5017..5760)
                     /locus_tag="O0286_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0171215.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            6186..>6633
                     /locus_tag="O0286_002943"
     CDS             6186..>6633
                     /locus_tag="O0286_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0171216.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENR"
BASE COUNT         2523 a          946 c         1023 g         2141 t
ORIGIN      
        1 tgttaaaatt atattataaa ttcaactttg caacagaacc gtattttatg agtacactct
       61 aaattaagga gatgtataat caatctaaaa ttgtattttt atacttttgt tatataatct
      121 ttctacttag agatatattg taaaaaaacc aacaaaatgt tatattaaca ttactatttt
      181 atcgatgaag gaggattaaa atgacagctt ctatgagact caaataaacc tttttgaata
      241 caaaactaaa ttaattcaaa aaggcttgtc cgcaatacac actcatagga tgacttatgt
      301 cattttttat ccttcgatag gaataataaa cgctttcaaa tactaaaaca tcataataaa
      361 tttttaagtg tttgaaagtg taatgtgaat gagacgggag tagcctgtct catttttttg
      421 cgtacacgtc ctttaaatat acaaggagtg tataaatatg gaacaatata caattaaatt
      481 taaccaaatc aatcataaat tgacagattt acgatcactt aacatcggtc atctttacgc
      541 ttaccaattt gaaaaaatag cacttattgg gggtaatggt actggcaaaa ccacattact
      601 aaatatgatt gctcaaaaaa caaaaccaga atctggaaca gttgaaacgg ttggcgaaat
      661 tcaatatttt gaacagctta acatggatgt ggaaaatgat tttaacacgt tagacggtag
      721 tttaatgagt gaactccata tacctatgca tacaaccgac agtatgagtg gtggtgaaaa
      781 agcaaaatat aaattagcta atgtcatatc aaattatagt ccgatattac ttttagatga
      841 acctacaaat cacttggata aaattggtaa agattatctg aaaaatattt taaaatatta
      901 ctatggtact ttaattatag taagtcatga tagagcactt atagaccaaa ttgctgacac
      961 aatttgggat atacaagaag atggcacaat aagagtgttt aaaggtaatt acacacagta
     1021 tcaaaatcaa tatgaacaag aacagttaga acaacaacgt caatatgaac agtatataag
     1081 tgaaaaacaa agattgtccc aagccagtaa agctaaacga aatcaagcgc aacaaatggc
     1141 acaagcatca tcaaaacaaa aaaacaaaag tatagcacca gatcgtttaa gtgcatcaaa
     1201 acaaaaaggc acggttgaga aggctgctca aaaacaagct aagcatattg aaaaaagaat
     1261 ggaacatttg gaagaagttg aaaaaccaca aagttatcat gaattcaatt tcccacaaaa
     1321 taaaatttat gatatccata ataattatcc aatcattgca caaaatctaa cattggttaa
     1381 aggaagtcaa aaactgctaa cacaagtacg attccaaata ccatatggca aaaatatagc
     1441 gctcgtaggt gcaaatggtg taggtaagac aactttactt gaagctattt accaccaaat
     1501 agagggaatt gattgttctc ctaaagtgca aatggcatac tatcgtcaac ttgcttatga
     1561 agacatgcgt gacgtttcat tattgcaata tttaatggat gaaacggatt catcagaatc
     1621 attcagtaga gctattttaa ataacttggg tttaaatgaa gcacttgatc gttcttgtaa
     1681 tgttttgagt ggtggggaaa gaacgaaatt atcgttagca gtattatttt caacgaaagc
     1741 gaatatgtta attttggatg aaccaactaa ttttttagat attaaaacat tagaagcatt
     1801 agaaatgttt atgaataaat atcctggaat cattttgttt acatcacatg atacaaggtt
     1861 tgttaaacat gtatcagata aaaaatggga attaacagga caatctcttc atgatataac
     1921 ttaaaaacta tacactaatt tatattgaat attaggttca tttatatcaa tataaaaaat
     1981 acgttcacaa gaaaaatata atagaaataa ggatccaata aaatgactcg acataatgaa
     2041 attattaaat gtgcagaaaa atatcaatta cacatccaac ctcaaacaat ctcattgaat
     2101 gaatcgggac ttgattttca agttgcattt ggaaaagata aacatggagt agaatgggtt
     2161 ttgagactac caagaagacc tgacgtttat aaacgaacaa aacccgaaaa acaaacggta
     2221 gacttcttac agaagaatgt ttcatttgaa ataccgaagt ggaaagtaca tgcaaaagac
     2281 cttattgctt acccaaaact tacaggtaaa cccgcagcca caatagatcc agaaatacaa
     2341 aattatgtat gggaaattga acacaaacca ttaccagaaa actttattaa cacattagct
     2401 gaaacactcg tagatttaca caacatacca gaagaaaaca ttaacgttca gcatataaat
     2461 atcaaaacca tacaagaaat aaaaaatgac tttcaaagaa gaatgaataa agttaaagaa
     2521 acttatggcg tatcagatga attatggaac agatggaaac aatggttaga aaacgacgaa
     2581 ctatggcctc gacatgcgac catgatacat ggggacttac atccaggaca tataatggta
     2641 gataaccaag caaacgtcac aggtctcata gactggactg aagcaaccca ctccgaccca
     2701 tcaatggact ttataggaca ccatcgtgta ttcgacgacg aaggattaga gcaacttata
     2761 acagcatacg gtaaagctgg aggtgaaata tggccacgaa tgaaagagca tataatagaa
     2821 ctcaatgcag tattcccaat gtttatcgct gagtttgcta tggaatcagg agaatcggcg
     2881 tatgaaacga tggcattgaa agagttaggt atgaaagagt agaacttata ttaaaagagg
     2941 cccattttaa agacgaaggc aatcctgatg aaattttaaa actgcgttta attagtgatg
     3001 gtctttggtt ttctataatg tattaaatat ctggacatgg aacaaatgca acaaatggaa
     3061 agaatcgttt tcgaaatttc taactcctta aatcaggagg ataaataaac ggtcattttc
     3121 attgaaagca cttatataaa tttcatcaat tttaatacct accgtaaata catttcataa
     3181 gtattaaaca gatgattaaa atttttagtt aatttaaata tttatgatag agatgacctt
     3241 caaatgaacg gttctgttac aaagaaaaaa tctatattta tttttagtaa gttttaatta
     3301 tagatatgta cactttgagt acaccctaac catacaaaaa gaattgttga aattataaca
     3361 attaaaacgt atgattaggg tgtatattta ttatggagtg aagatgaatg attataggtt
     3421 atgcgagagt atcatcgata gatcaaaatt tagaaagaca attggataat ttaaagacgt
     3481 ttggtgtgga gaaaatcttt acagagaaac agtcgggaaa gtcagtggaa aatagacctg
     3541 tatttcaaga agcactcaat tttgtcagaa tgggagatcg attcgttgtg gaatctattg
     3601 atcgtttagg acgtaactat gatgaaatta ttgaaactgt gaattattta aaagaaaaag
     3661 acgttcagtt aatgattacc agcttaccta tgatgaatga agtaattggc aatccattac
     3721 tggataagtt tatgaaagat ttaatcattc aaatattagc aatggtttca gaacaagaga
     3781 gaaatgaaag taaacgtcga caagcgcaag ggattcaagt tgcgaaggaa aaaggcgtat
     3841 ataaagggcg ccctttactt tattcaccaa atgctaaaga cccacagaaa cgtgttattt
     3901 atcatcgcgt ggtggaaatg ttagaagaag gcaaagcgat tagtaagatt gcgaaagaag
     3961 ttaatatcac aagacaaaca gtttatagaa ttaaacatga taagggatta tcttgataaa
     4021 ttgattgtca atgcaaatga gcgtctctca cagtgtctca aattccataa catattattg
     4081 aattgagatt ttaataatta tacaagaaaa aagagtacga gttcgcagct ttctatatag
     4141 taattgtttt actaataagg tgttttaaaa tatattataa tgtaaaaagt atttgacatt
     4201 tgtatcaaaa atatttacac tcataaatgt caaatacttt ttacatttat gaggtgttat
     4261 aatgaattta attcctttta ttattatttt aattttttct tgtttatgga cggctataac
     4321 taacaaatat ttaccttcag aaacattaga taaaaaagga caacaagatc gatacgatga
     4381 aagacaacgt aaaatgttta ttgaaatact ggctaaaagt tttatttgga ttgtatattg
     4441 tatgttattt acactagtgc ttaaatttac aggtttgtca ccaagtattg aaggatcttg
     4501 gttttctcaa tatccagaaa ttttctttat aattgtagca ctatttctta tgctgtttaa
     4561 ttactatact acaaataaaa aatatacatc taaaggttaa attaatgaaa aatagaatta
     4621 gagaatatag aaaacaaaaa ggaatttctc aagaaacttt aagtaaagaa ttaaaagtgt
     4681 ctaggcaaac tattaatgca atagaaaaca ataaatatga tcctacattg acattagcat
     4741 ttaagttggc aagattgttt gatacaactg tggatgaatt attttcttaa ccattcattt
     4801 atcacaaaaa gagagtgcga attcgcactc tctttttgtg tagtagtact attaaatttt
     4861 gtctcaataa cgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt
     4921 tgaaatactt tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac
     4981 taaaatttta taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca
     5041 aaaccccttt ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt
     5101 tagattagct ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg
     5161 acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt
     5221 aatatttaca ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt
     5281 agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt
     5341 ttgaatcttt ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct
     5401 ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc
     5461 aatttgatta tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct
     5521 tttcgattca aaattaatat tatccgaccc ttttttttgt ttgtcttcag tagtactttc
     5581 aaaaccatat ttctttttat tttcatttac tctttgaatg attttttcta tatcggtatc
     5641 ttctttaata aacgaagtgt tttcctgttt tattgtctct atattcaatc gcttaatatt
     5701 attaaaaatc gtttgcttac taacccctaa ttcttcactc aactttttca cacttttcat
     5761 atcatcaacc ctttataaaa tgtttataaa cccttgataa aatattataa gcttataatg
     5821 cttaataata aagtgattaa taaataaaaa taaacatcca tcaacctttt tatcaagggt
     5881 ttatgaaagg tttatgatat ttatcaaggg tttataaacc cttgataaag ctatattttc
     5941 ccttttataa gtttatttaa agtgctataa taaacttaat ataacaaaaa ccccaactac
     6001 tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat ataattttcg
     6061 atacttgtat tatataatga ataatatctt agtgcaagca cctaaatcat taaataaatg
     6121 taaagctaaa taaaaacgtt ttattacctc ttaccaaact aaaatatgga aagaggtttt
     6181 ttgtcatgca aaatcaatat tttacagtgc aagaaaatta caaagaaaga ttttatcaaa
     6241 tccctaaagt attctttacc agtgaaaatt ataaaaatct aactaatgat atgaaaatag
     6301 cctatgctat cttacgtgat cgcttaaatt tatctattaa aaatagctgg gttgatgaag
     6361 atggtaacat ttactttgtt tattcaaatg aaaagctaat ggaaatctta aattgtaaaa
     6421 aagaaaagtt aacaaaaata aaaaaagggt tagaaaacga tggtctactc attcaaaaac
     6481 gacgtggttt aaataaacca aatattctat atttaatgaa acctattgtc acagaacgtg
     6541 atatttacaa aatagaaaaa gaagaaaacg atgttgaacc ttacggcgag aaggaagttc
     6601 gaaaatcgaa cgtccagaag ttcgaaaatc gaa
//
LOCUS       DAKTGS010000306         6197 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.153, whole genome shotgun sequence.
ACCESSION   DAKTGS010000306 DAKTGS010000000
VERSION     DAKTGS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.153"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            7..963
                     /locus_tag="O0286_002944"
     CDS             7..963
                     /locus_tag="O0286_002944"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0171217.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1163..1642
                     /locus_tag="O0286_002945"
     CDS             1163..1642
                     /locus_tag="O0286_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0171218.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1657..2496
                     /gene="fakB2"
                     /locus_tag="O0286_002946"
     CDS             1657..2496
                     /gene="fakB2"
                     /locus_tag="O0286_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0171219.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2582..3115
                     /gene="msrA"
                     /locus_tag="O0286_002947"
     CDS             2582..3115
                     /gene="msrA"
                     /locus_tag="O0286_002947"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0171220.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3108..3536
                     /gene="msrB"
                     /locus_tag="O0286_002948"
     CDS             3108..3536
                     /gene="msrB"
                     /locus_tag="O0286_002948"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCY0171221.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3548..4048
                     /locus_tag="O0286_002949"
     CDS             3548..4048
                     /locus_tag="O0286_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0171222.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4048..4269
                     /locus_tag="O0286_002950"
     CDS             4048..4269
                     /locus_tag="O0286_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCY0171223.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4461..5951
                     /locus_tag="O0286_002951"
     CDS             4461..5951
                     /locus_tag="O0286_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCY0171224.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
BASE COUNT         2326 a          874 c         1108 g         1889 t
ORIGIN      
        1 tcgcatatgt tgaattcatt tgatgcagca tatcacagtc tttgtgaaga agttttagaa
       61 ataggaaata cacgaaatga tcgcacaaat acaggtacga tttcgaaatt tggtcatcaa
      121 cttcgctttg acttatctaa aggatttcca ctattaacga caaagaaagt ttcttttaaa
      181 ttagtagcaa ccgaattatt atggttcatt aaaggagata caaacatcca atacttatta
      241 aaatataata ataatatatg gaacgaatgg gcttttgaaa attatatcaa atcagacgag
      301 tataaaggtc cagatatgac agatttcggg catcgtgcat tgagtgatcc tgaatttaac
      361 gaacaatata aagaacaaat gaaacaattt aagcaacgta ttcttgaaga tgatacattt
      421 gcgaagcaat tcggggattt aggaaatgtt tatggtaaac aatggcgaga ttgggttgat
      481 aaagatggta atcattttga tcaacttaaa acagtaattg aacaaattaa gcataatcca
      541 gattcaaggc gacacatcgt atctgcatgg aatccaacag aaattgatac aatggcactt
      601 ccgccttgtc ataccatgtt ccagttttat gtccaagatg gtaagttaag ttgccagtta
      661 taccaacgta gcgcagatat ctttttaggt gtgccattta atatcgcaag ctacgcttta
      721 ttgacacacc ttattgccaa agaatgtgga cttgaagtgg gtgaatttgt gcatacattt
      781 ggagatgcac atatttattc aaatcatatt gatgcgattc aaacacaatt agcacgtgaa
      841 agcttcaatc ctccaacatt aaaaattaac agtgacaagt ctattttcga cataaattat
      901 gaagatttgg aaattgttga ctatgaatca catccagcaa taaaagctcc aatagcagtg
      961 tagtcattgc atagttagct aaccatatag acatcaaaat gacatcatag tattttcaag
     1021 tgcaaaaaag tacttttttg tgttaaacgt tttcataaat tatgcaaaat cattatttct
     1081 atcacacttt atgataaaaa ttgtgttaaa ttaaagataa cttagtaata aaaaatgaaa
     1141 tgatagaaga aggaggataa ttatgacttt atccattcta gttgcacatg acttgcaacg
     1201 agtaattggt tttgaaaatc aattaccttg gcacctacca aatgatttga agcatgttaa
     1261 aaaattatca acaggtcata ctttagtaat gggtcgtaag acatttgaat cgattggtaa
     1321 accactaccg aatcgtcgaa atgttgtact tacttcagat acaagtttca acgtagaggg
     1381 cgttgatgta attcactcta ttgaagatat ttaccaacta ccgggccatg ttttcatatt
     1441 tggagggcaa acattatttg aagaaatgat tgataaagtg gacgacatgt atattactgt
     1501 tattgaaggt aaattccgtg gtgatacgtt ctttccacct tatacatttg aagactggga
     1561 agttgcctct tcagttgaag gtaaactaga tgagaaaaat acaattccac atacctttct
     1621 acatttaatt cgtaaaaaat aagggggaaa acgaccatga caaaacagat tatagtaaca
     1681 gactcaacat ccgatttatc taaagaatac ttagaagcaa acaacattca tgtaattcct
     1741 ttaagtttaa ctattgaagg agcttcatac gttgaccaag tagatattac atcagaagaa
     1801 tttattaatc atattgaaaa tgatgaagat gtaaagacaa gtcagccagc cataggtgaa
     1861 tttatatctg cttatgaaga actaggaaaa gatggctctg aaatcataag tattcatctt
     1921 tcttcaggat taagtggtac atataacact gcttaccaag caagtcaaat ggtagatgct
     1981 aatgtaactg ttattgattc aaaatctatt tcttttggtt tagggtatca aatacaacac
     2041 ctagtagagc ttgtaaaaga aggtgtctca acttctgaaa tagttaaaaa gttaaatcat
     2101 ttaagagaaa acattaaatt atttgtagtt atagggcaat tgaatcaatt aattaaaggt
     2161 ggcagaatta gtaaaacaaa aggtttgatt ggtaatctta tgaaaattaa accaattggt
     2221 acactagatg atggtcgctt agagcttgtg cacaatgcga gaactcaaaa ttctagtatc
     2281 caatacttga aaaaggaaat tgctgaattt ataggagatc atgaaatcaa atccattggt
     2341 gtcgcacatg ctaacgtcat tgaatatgtt gataaattga agaaagtttt taatgaagct
     2401 tttcatgtga ataattacga tataaatgta actacaccag ttatttctgc acatactggt
     2461 caaggtgcga ttggcctcgt agtccttaag aagtaaattt aatcttttca gtgttaatta
     2521 cttccatttc aatcctttat agactaaatt tataattaga tagatagagg aggtaattca
     2581 tatgacaaaa gaatatgcaa cattagcagg aggatgtttc tggtgcatgg ttaaaccatt
     2641 tacatcatat ccaggcatca agtcagtcgt atctggttat agtggcggtc atgttgacaa
     2701 cccaacttat gaacaggtat gtacgaatca aaccggccat gtcgaagcag tacaaattac
     2761 gtttgatcca gaggttactt cctttgaaaa tatattagac atatatttca aaacatttga
     2821 cccaactgat gatcaagggc aatttttcga tagaggcgaa agctatcaac cagtcatttt
     2881 ctatcatgat gaacatcaga aaaaggctgc tgagtttaaa aagcaacaat taaatgaaca
     2941 aggtattttc aagaaaccag tgattacacc tattaaacca tataaaaatt tctatccagc
     3001 tgaagactac catcaagatt attacaaaaa gaacccggta cattattacc aatatcaacg
     3061 tggttcaggt agaaaagcgt ttatagaatc acattggggg aatcaaaatg cttaaaaaag
     3121 ataaaagtga actaacagat atagaatata ttgttacaca agaaaacggc actgaaccac
     3181 catttatgaa tgaatattgg aatcattttg ctaaaggaat ttatgtagat aaaatttctg
     3241 gtaaaccttt atttacatct gaagaaaagt ttcattctga atgtggatgg cctagctttt
     3301 ccaaagcgct tgatgacgat gaaattatag aattagtcga caaatcattt ggtatgttga
     3361 gaactgaagt gcgttcagaa gaatcaaata gtcatttagg acatgtcttt aatgatggac
     3421 ctaaagaaag tggcggctta agatactgta tcaattccgc tgcaattcaa tttattccat
     3481 atgaaaagtt agaagaattg ggttatggcg atttaatatc acattttgat aagtaggagt
     3541 ggaaagtatg tttaaaaaat tattcggaaa aggcaaagaa gttcagaaag atatcgcaat
     3601 ttatgcacca ttaactggag aattcgtgaa aattgaagat attccagatc ctgtattcgc
     3661 acaaaaaatg atgggcgaag gttttggtat taatccaact gaaggagaag ttgtgtctcc
     3721 aattgcagga cgtgttgaca atgtctttcc aactaagcat gcgattgggt taaaagcaga
     3781 taacggatta gaattattag ttcatatcgg tttagacaca gttcaattag acggtgaagg
     3841 atttgaagtg ttagtgtcta gtggtgacga agttaacgtc ggagatccat tagtaagatt
     3901 caaccttgaa tatattaata ataacgctaa atctgtaatt tcaccaatta taattactaa
     3961 cactgatcaa gcagcttcaa ttaatattta tgatgaaaat gctgtgatta aaggtgaaac
     4021 aaaagtgatt gatgtgacaa tgaactaatg aaaaactatt cgttttatca atttgtcatg
     4081 acagttcgtg gtcgacacga cgataaaggt cgtctagcag aagagatatt tgacgatctt
     4141 gctttcccaa aacacgatga tgattttaac atactgtctg attatattga gacacatggt
     4201 gatttcacat tgccaatgtc tgtatttgat gatttatatg aagaatatac ggaatggcta
     4261 aaattttaat ataattttta ataataatag ttagaaccag ggtgatgcaa ttcgttatcc
     4321 tggttttaat ttaaaataaa ctaagttgtg actaaaaatt aatcaattat agtgaaatat
     4381 ggtgcgctat cttgcataaa ttgatatgat taactacaca gaatttaaaa gtacataata
     4441 cataataagg aagtgataca atggatgata agcaacacac atcttcatcc gatgatgaac
     4501 gcgctgaaat tgcaacaagc aatcaagacc aagaaactaa ttcatcgaaa cgcgttcact
     4561 taaaacgttg gcaattcata tcaatattaa ttggtacaat cctaataaca gctgtcatca
     4621 cagttgttgc atatattttt ataaatcaaa aaataagtgg tttaaacaaa actgatcaag
     4681 caaacttaaa taaaattgaa aatgtgtata aaatcttaaa tagtgattat tacaaaaaac
     4741 aggactctga caagttaagt aaagctgcaa ttgatggcat ggtcaaagaa ttaaaagatc
     4801 cttattctga atatttaaca aaagaacaaa cgaaatcctt taatgaaggt gtttcaggtg
     4861 attttgtagg tattggtgca gaaatgcaaa agaaaaatga tcaaattatg gttactagtc
     4921 ctatgaaggg atctccagca gaacgtgctg gcattcgtcc taaagatgtc attactaaag
     4981 taaatggaaa atcaattaaa ggtaaagcat tagatgaagt tgtcaaagat gttcgtggta
     5041 aagaaaacac tgaagtcact ttaactgttc aacgaggtag tgaagaaaaa gacgttaaga
     5101 ttaaacgtga aaaaattcat gttaaaagtg ttgagtataa gaaaaaaggt aaagttggag
     5161 ttattactat taataaattc cagaatgata catcaggtga attgaaagat gcagttctaa
     5221 aagctcacaa agatggtttg aaaaagattg ttttagattt aagaaataat ccaggtggac
     5281 tactagatga agctgttaaa atggcaaata tttttatcga taaaggaaaa actgttgtta
     5341 aactagaaaa aggtaaagat actgaagcaa ttcaaacttc taatgatgcg ttaaaagaag
     5401 cgaaagacat ggatatatcc atcttagtga atgaaggttc agctagcgct tctgaagtgt
     5461 ttactggtgc gctaaaagac tataataaag ctaaagttta tgggtcaaaa acattcggca
     5521 aaggtgtcgt acaaactaca agagagttta aggatggttc attgttaaaa tatactgaaa
     5581 tgaaatggtt aacgccagat ggtcattata ttcacggtaa aggcattaaa ccagacgtta
     5641 ctattgacac acctaaatat caatctttaa atgtcattcc taatacgaaa acatttaaag
     5701 ttggagacga tgataaaaat attaaaacta ttaaaattgg tttatcagct ttaggttata
     5761 aagttgataa tgaatcaacg caatttgata aagctttaga aaatcaagtt aaagctttcc
     5821 aacaagcgaa taaacttgag gtaactggtg aatttaataa agaaacgaat aataaattta
     5881 ctgagttatt agttgaaaaa gctaataaac atgatgatgt tctcgataag ttgattaata
     5941 ttttaaaata agcgatacac actactaaaa ttgtattatt attatgttaa tgacacgcct
     6001 cctaaatttg caaagatagc aatttaggag gcgtgtttat ttttattgac gtctaactct
     6061 aaaagatata aattagacat ttacaaatga tgtaaataac gcaatttcta tcatcgctga
     6121 taacaattca tggtttaata tgcaatgagc atatactttt taaatagtat tattcactag
     6181 ttttaacaat caattaa
//
LOCUS       DAKTGS010000307         5357 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.163, whole genome shotgun sequence.
ACCESSION   DAKTGS010000307 DAKTGS010000000
VERSION     DAKTGS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.163"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..254
                     /locus_tag="O0286_002952"
     CDS             <1..254
                     /locus_tag="O0286_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCY0171225.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
     gene            complement(245..505)
                     /locus_tag="O0286_002953"
     CDS             complement(245..505)
                     /locus_tag="O0286_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0171226.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(477..1463)
                     /locus_tag="O0286_002954"
     CDS             complement(477..1463)
                     /locus_tag="O0286_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0171227.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1560..3569
                     /gene="mecA"
                     /locus_tag="O0286_002955"
     CDS             1560..3569
                     /gene="mecA"
                     /locus_tag="O0286_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0171228.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(3615..4043)
                     /locus_tag="O0286_002956"
     CDS             complement(3615..4043)
                     /locus_tag="O0286_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0171229.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(4140..4883)
                     /locus_tag="O0286_002957"
     CDS             complement(4140..4883)
                     /locus_tag="O0286_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0171230.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1882 a          867 c          788 g         1820 t
ORIGIN      
        1 ctgtttttgg atttatgaag gctattttgg gtttcactcg gatgtctgtc cgaggactca
       61 ataaagtcaa aagagaactt ggttttgtat taatggcact taatataaga aaagtagtag
      121 ctcaacgagc tgaaaataat caaaaaattt ataaaaaaga caatttctat attatttcaa
      181 tagaaattgt cttttttcac ttatccaaga actttatgtc ccggactcat tatgatttat
      241 ttttttattg ctgacttgcc attaattctg cttgctctac aacttgttcc acagccattt
      301 tttgaagatc tggtggatag ccatatttct ttaataggcg tctaacttga actctcattt
      361 tagctttagc actgtctcgt ttagaccaat caacactcat gttatcctta actgttttag
      421 ttaactcatg tgcaatggct cttaactctt tatcgcccaa agcttcttta gctgtttcat
      481 gtgaagctcg atatactaca tacaatatac gcaacaatac gcttgtttcg attaggttta
      541 ggcattgaat cataaagtgc aatatactta acacgttctt taatatttga attaaaacct
      601 agtaaatatt gtgctgccac attatttatg tgctgagatt ttaaaataga gcattttaat
      661 atcgattcac cataacgtat atgttcatgg cgattcaaaa tttttaaaac gtttctatca
      721 catacttttt cacagtcatt gtccatcatt gttttactta tatatagtgc aggattaaac
      781 cagaatatca ttttaaaaac aacataaagc tggttgaata ttaagtcatg acttttcaca
      841 tgtgatagtt catgtagaat aatatattca atttctttgt cattcatggt ttcgactacg
      901 acagttggta gtacaatttg ggatttcact aaaccaaata ccatcggatt atcaatgttt
      961 gaactataac taattgttat atgctttttg tagaactgca tcttactttg acatacttta
     1021 agtcgttcat taagatatga cgattccaat gacgaacttt taataacatc aatttgtcgg
     1081 aatgccttaa tcatataaaa taagcacaac aaactaccaa atacccatat caaaagaatc
     1141 atatacgtta tatttgaggt ctcaaactga ttaacattaa ttgctaagtc tttcgtaaca
     1201 gatgattgtt gaccatctaa catatgacta accgaagaag tcgtgtcaga tacatttcga
     1261 ttcatcatat cttttgaaaa tgtaaaattc gatattttgt aaaatggtat taatggaatt
     1321 aacgtggaga cgagcactaa taaccaaatc ttatgtgaca taatattttg agtatatttt
     1381 atatagagca ttctcactaa aaaaattaca catatcgtga gcaatgaact gattatactt
     1441 aacattaaaa aagatgataa caccttctac acctccatat cacaaaaatt ataacattat
     1501 tttgacataa atactacatt tgtaatatac tacaaatgta gtcttatata aggagtatat
     1561 tgatgaaaaa gataaaaatt gttccactta ttttaatagt tgtagttgtc gggtttggta
     1621 tatattttta tgcttcaaaa gataaagaaa ttaataatac tattgatgca attgaagata
     1681 aaaatttcaa acaagtttat aaagatagca gttatatttc taaaagcgat aatggtgaag
     1741 tagaaatgac tgaacgtccg ataaaaatat ataatagttt aggcgttaaa gatataaaca
     1801 ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa acgagtagat gctcaatata
     1861 aaattaaaac aaactacggt aacattgatc gcaacgttca atttaatttt gttaaagaag
     1921 atggtatgtg gaagttagat tgggatcata gcgtcattat tccaggaatg cagaaagacc
     1981 aaagcataca tattgaaaat ttaaaatcag aacgtggtaa aattttagac cgaaacaatg
     2041 tggaattggc caatacagga acagcatatg agataggcat cgttccaaag aatgtatcta
     2101 aaaaagatta taaagcaatc gctaaagaac taagtatttc tgaagactat atcaaacaac
     2161 aaatggatca aaattgggta caagatgata ccttcgttcc acttaaaacc gttaaaaaaa
     2221 tggatgaata tttaagtgat ttcgcaaaaa aatttcatct tacaactaat gaaacagaaa
     2281 gtcgtaacta tcctctagga aaagcgactt cacatctatt aggttatgtt ggtcccatta
     2341 actctgaaga attaaaacaa aaagaatata aaggctataa agatgatgca gttattggta
     2401 aaaagggact cgaaaaactt tacgataaaa agctccaaca tgaagatggc tatcgtgtca
     2461 caatcgttga cgataatagc aatacaatcg cacatacatt aatagagaaa aagaaaaaag
     2521 atggcaaaga tattcaacta actattgatg ctaaagttca aaagagtatt tataacaaca
     2581 tgaaaaatga ttatggctca ggtactgcta tccaccctca aacaggtgaa ttattagcac
     2641 ttgtaagcac accttcatat gacgtctatc catttatgta tggcatgagt aacgaagaat
     2701 ataataaatt aaccgaagat aaaaaagaac ctctgctcaa caagttccag attacaactt
     2761 caccaggttc aactcaaaaa atattaacag caatgattgg gttaaataac aaaacattag
     2821 acgataaaac aagttataaa atcgatggta aaggttggca aaaagataaa tcttggggtg
     2881 gttacaacgt tacaagatat gaagtggtaa atggtaatat cgacttaaaa caagcaatag
     2941 aatcatcaga taacattttc tttgctagag tagcactcga attaggcagt aagaaatttg
     3001 aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc aagtgattat ccattttata
     3061 atgctcaaat ttcaaacaaa aatttagata atgaaatatt attagctgat tcaggttacg
     3121 gacaaggtga aatactgatt aacccagtac agatcctttc aatctatagc gcattagaaa
     3181 ataatggcaa tattaacgca cctcacttat taaaagacac gaaaaacaaa gtttggaaga
     3241 aaaatattat ttccaaagaa aatatcaatc tattaactga tggtatgcaa caagtcgtaa
     3301 ataaaacaca taaagaagat atttatagat cttatgcaaa cttaattggc aaatccggta
     3361 ctgcagaact caaaatgaaa caaggagaaa ctggcagaca aattgggtgg tttatatcat
     3421 atgataaaga taatccaaac atgatgatgg ctattaatgt taaagatgta caagataaag
     3481 gaatggctag ctacaatgcc aaaatctcag gtaaagtgta tgatgagcta tatgagaacg
     3541 gtaataaaaa atacgatata gatgaataac aaaacagtga agcaatccgt aacgatggtt
     3601 gcttcactgt tttattatga attattaata agtgctgtta cttctccctt aaatacaatt
     3661 tcttcatttt cattgtatgt tgaaagtgac actgtaacga gtccattttc tttttttatg
     3721 gatttcttat ttgtaatttc agcgataacg tacaatgtat tacctgggta tacaggttta
     3781 ataaatttaa cgttattcat ttgtgttcct gctacaactt cttctccgta tttaccttct
     3841 tctacccata atttaaatga tattgaaagt gtatgcatgc cagatgcaat gataccttta
     3901 aatctacttt gttctgcttt ttctttatct atatgcatat attgaggatc aaaagttgtt
     3961 gcaaattgga taatttcttc ttctgtaata tgaaggcttt ttgttttgaa tgtttctcct
     4021 actataaaat catcgtattt catatatgtc tctctttctt attcaaatta attttttagt
     4081 atgtaacatg ttaaaggtaa gtctaccgtc actgaaacgt aagactcacc tctaactttc
     4141 tattgagaca aatgcaccat tttatctgca ttgtctgtaa agataccatc aactccccaa
     4201 ttagcaagtt ggtttgcacg tgctggtttg tttacagtcc atacgttcaa ttcataaccc
     4261 gcttctttta ccatttttac ttttgcttta gtaagtttgg catcttcagt gtttactatt
     4321 ttagcattac agtaatctaa aagtgttctc cagtcttcac gaaacgaagt tgtatggaat
     4381 ataactgctc tgttatattg tggcatgatt tcttctgcaa gtttaacaag cacaacatta
     4441 aagcttgaaa tgagcacttc ttgattctga tttaagtttg ttaattgttc ttccacttgc
     4501 ttaaccatac ttttagaaag tgctagtcca ttcggtccag taataccttt taattctaca
     4561 tttaaattca tattatattc atttgctatt tttactacat catcgaaagt tggcaaatgt
     4621 tcatctttga atttttcacc aaaccaagat cctgcagaag catctttaat ttcatcataa
     4681 ttcaattcag ttatttcccc ggacatattt gtagtccgtt ctaaataatc atcatgaatg
     4741 ataatcagtt gttcatcttt tgtaattgca acatctaact ccaaccagtt tataccttct
     4801 acttctgaag cagctttaaa tgatgcaatt gtattttccg gagctttact aggtaatcct
     4861 ctatgtccat atacagttag catattacct ctccttgcat ttttattttt ttaattaacg
     4921 taactgtatt atcacattaa tcgcactttt atttccatta aaaagagatg aatatcataa
     4981 ataaagaagt cgatagattc gtattgatta tggagttaat ctacgtctca tctcattttt
     5041 aaaaaatcat ttatgtccca agctccattt tgtaatcaag tctagttttt ctgtacccct
     5101 tatctgcaat tttacttagg attgctttta acttacccct tatcagcaat tttactgaga
     5161 actgctttta acgcacctct tatctgcaat tttgcctaga actgctttta acgtacctct
     5221 tatctgcaat tttactgaga actgctttta acttacccct tatcagcaat tttgcatgga
     5281 attgctttta acgtacctct tatctgcaat tttacttaga actgctttta acaaacctct
     5341 tatctgcaat tttactt
//
LOCUS       DAKTGS010000308         4479 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.176, whole genome shotgun sequence.
ACCESSION   DAKTGS010000308 DAKTGS010000000
VERSION     DAKTGS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.176"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            1..1380
                     /gene="tet(K)"
                     /locus_tag="O0286_002958"
     CDS             1..1380
                     /gene="tet(K)"
                     /locus_tag="O0286_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(K)"
                     /protein_id="HCY0171231.1"
                     /translation="MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHF
                     NTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNH
                     FFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGG
                     IIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFT
                     TNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFI
                     SMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSIS
                     ISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFT
                     SFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIV
                     FKRSKKQFE"
     gene            1566..2807
                     /locus_tag="O0286_002959"
     CDS             1566..2807
                     /locus_tag="O0286_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCY0171232.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLN
                     YDLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQ
                     FFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALT
                     AFQDRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIK
                     QKNDKLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQK
                     QIKAAQDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKP
                     LKENIEIALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELY
                     SEQEQQQEQQKNQKRDRGMHL"
     gene            3334..4278
                     /locus_tag="O0286_002960"
     CDS             3334..4278
                     /locus_tag="O0286_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCY0171233.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLS
                     FDAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADS
                     WDRRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDK
                     AVKKTIFYGRNGKPETKYFGVRDSNRFIRIYNKKKERKDNADAEVMSEHLWRVEIELK
                     RDMVDYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLI
                     KEISPVDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
BASE COUNT         1665 a          541 c          796 g         1477 t
ORIGIN      
        1 ttgtttagtt tatataaaaa atttaaaggt ttgttttata gcgttttatt ttggctttgt
       61 attctttcat tttttagtgt attaaatgaa atggttttaa atgtttcttt acctgatatt
      121 gcaaatcatt ttaatactac tcctggaatt acaaactggg taaacactgc atatatgtta
      181 actttttcga taggaacagc agtatatgga aaattatctg attatataaa tataaaaaaa
      241 ttgttaatta ttggtattag tttgagctgt cttggttcat tgattgcttt tattggtcac
      301 aatcactttt ttattttgat ttttggtagg ttagtacaag gagtaggatc tgctgcattc
      361 ccttcactga ttatggtggt tgtagctaga aatattacaa gaaaaaaaca aggcaaagcc
      421 tttggtttta taggatcaat tgtagcttta ggtgaagggt taggtccttc aataggggga
      481 ataatagcac attatattca ttggtcttac ctacttatac ttcctatgat tacaatagta
      541 actatacctt ttcttattaa agtaatggta cctggtaaat caacaaaaaa tacattagat
      601 atcgtaggta ttgttttaat gtctataagt attatatgtt ttatgttatt tacgacaaat
      661 tataattgga cttttttaat actcttcaca atcttttttg tgatttttat taaacatatt
      721 tcaagagttt ctaacccttt tattaatcct aaactaggga aaaacattcc gtttatgctt
      781 ggtttgtttt ctggtgggct aatattttct atagtagctg gttttatatc aatggtgcct
      841 tatatgatga aaactattta tcatgtaaat gtagcgacaa taggtaatag tgttattttt
      901 cctggaacca tgagtgttat tgtttttggt tattttggtg gttttttagt ggatagaaaa
      961 ggatcattat ttgtttttat tttaggatca ttgtctatct ctataagttt tttaactatt
     1021 gcattttttg ttgagtttag tatgtggttg actactttta tgtttatatt tgttatgggc
     1081 ggattatctt ttactaaaac agttatatca aaaatagtat caagtagtct ttctgaagaa
     1141 gaagttgctt ctggaatgag tttgctaaat ttcacaagtt ttttatcaga gggaacaggt
     1201 atagcaattg taggaggttt attgtcacta caattgatta atcgtaaact agttctggaa
     1261 tttataaatt attcttctgg agtgtatagt aatattcttg tagccatggc tatccttatt
     1321 attttatgtt gtcttttgac gattattgta tttaaacgtt ctaaaaagca gtttgaatag
     1381 ttatattata ttttggttta gaactatgag tggctagcat tttgccactc attttttgcg
     1441 ttagcaaaaa caggtttaag cctcgcagag cacacgtatt aacgacttat taaaaataag
     1501 tctagtgtgt tagacttaaa ctattaaata cacatgaaac ctttgtgctt aggagtgatt
     1561 tttatatgtc ttattccatt gttagagttt caaaagttaa atctggaaca aatacaacgg
     1621 gcatacaaaa acatgttcaa agagaaaata ataattatga aaatgaagat atagaccata
     1681 gtaaaactta cttaaattat gatttggtaa atgctaataa acagaatttt aataacttga
     1741 ttgatgaaaa aatcgaacag aattatacag gcaaaagaaa aattagaaca gacgcgatta
     1801 aacacattga tggtttaatt acatcagaca atgatttctt tgataatcaa acgccagaag
     1861 atacaaagca gttttttgaa tatgctaaag agtttttaga acaagaatac ggtaaagata
     1921 atttattata tgcaacagtt cacatggacg aaaaaacacc acatatgcat tatggcgttg
     1981 ttccaataac tgatgatggt cgtttaagtg ctaaagaagt tgtaggtaat aaaaaagctt
     2041 taacagcgtt tcaagataga tttaatgagc atgttaaaca acgaggatat gatttagaac
     2101 gtgggcaatc aagacaagta acaaatgcta aacatgagca aataagtcag tataaacaaa
     2161 aaacagaata tcataagcaa gaatatgaac gtgagagcca aaaaacagac catataaagc
     2221 aaaagaacga taaattaatg caagagtacc aaaaatcgtt aaatacgctt aaaaagccta
     2281 taaatgttcc gtatgagcaa gaaactgaaa aagtaggtgg tttatttagc aaagaaatac
     2341 aagaaactgg aaatgttgta ataagccaaa aagatttcaa tgaatttcag aaacagataa
     2401 aagctgctca agatatttcg gaagattacg agtatataaa gtctggtaga gccttagatg
     2461 ataaagataa ggaaatacga gagaaagatg atttattaaa taaagcagtt gagcgtattg
     2521 aaaacgcaga cgataatttt aaccaacttt acgaaaatgc aaagccactt aaagagaata
     2581 tagaaatagc gttaaagctt ttaaaaatct tactaaaaga gttagaacga gttttaggaa
     2641 gaaatacctt tgcggaaaga gttaataagt taacagaaga tgaaccaaaa ctaaatggtt
     2701 tagcaggaaa cttagataaa aaaatgaatc cagaattata ttcagaacag gaacagcaac
     2761 aagaacaaca aaagaatcaa aaacgagata gaggtatgca cttatagaac atgcatttat
     2821 gccgagaaaa cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt
     2881 tggacttgaa ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt
     2941 aggttccgac caataaggaa attggaataa agcaataaaa ggagttgaag aaatgaaatt
     3001 cagagaagcc tttgagaatt ttataacaag taagtatgta cttggtgttt tagtagtttt
     3061 aactgtttac cagataatac aaatgcttaa ataaaaaaag acttgatctg attagaccaa
     3121 gtcttttgat agtgttatat taataacaaa ataaaaagga gtcgctcacg ccctgaccaa
     3181 agtttgtgaa cgacatcatt caaagaaaaa aacactgagt tatttttata atcttgtata
     3241 tttagatatt aaacgatatt taaatataca tcaagatata tatttgggtg agcgattcct
     3301 taaacgaaat tgagattaag gagtcgattt tttatgtata aaaacaatca tgcaaatcat
     3361 tcaaatcatt tggaaaatca cgatttagac aatttttcta aaaccggcta ctctaatagc
     3421 cggttggacg cacatactgt gtgcatatct gatccaaaat taagttttga tgcaatgacg
     3481 atcgttggaa atctcaaccg agacaacgct caagcccttt ctaaatttat gagtgtagag
     3541 ccccaaataa gactttggga tattcttcaa acaaagttta aagctaaagc acttcaagaa
     3601 aaagtttata ttgaatatga caaagtgaaa gcagatagtt gggatagacg taatatgcgt
     3661 attgaattta atccaaacaa acttacacga gatgaaatga tttggttaaa acaaaatata
     3721 ataagctaca tggaagatga cggttttaca agattagatt tagcctttga ttttgaagat
     3781 gatttgagtg actactatgc aatgtctgat aaagcagtta agaaaactat tttttatggt
     3841 cgtaatggta agccagaaac aaaatatttt ggcgtgagag atagtaatag atttattaga
     3901 atttataata aaaagaaaga acgtaaagat aatgcagatg ctgaagttat gtctgaacat
     3961 ttatggcgtg tagaaatcga acttaaaaga gatatggtgg attactggaa tgattgcttt
     4021 agtgatttac atatcttgca accagattgg aaaactatcc aacgcactgc ggatagagca
     4081 atagttttta tgttattgag tgatgaagaa gaatggggaa agcttcacag aaattctaga
     4141 acaaaatata agaatttgat aaaagaaatt tcgccagtcg atttaacgga cttaatgaaa
     4201 tcgactttaa aagcgaacga aaaacaattg caaaaacaaa tcgatttttg gcaacatgaa
     4261 tttaaatttt ggaaatagtg tacatattaa tattactgaa caaaaatgat atatttaaac
     4321 tattctaatt taggaggatt tttttatgaa gtgtctattt aaaaatttgg ggaatttata
     4381 tgaggtgaaa gaataattta cccctataaa ctttagtcac ctcaagtaaa gaggtaaaat
     4441 tgtttagttt atataaaaaa tttaaaggtt tgttttata
//
LOCUS       DAKTGS010000309         3299 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.194, whole genome shotgun sequence.
ACCESSION   DAKTGS010000309 DAKTGS010000000
VERSION     DAKTGS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.194"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            39..416
                     /locus_tag="O0286_002961"
                     /pseudo
     CDS             39..416
                     /locus_tag="O0286_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            413..1294
                     /locus_tag="O0286_002962"
                     /pseudo
     CDS             413..1294
                     /locus_tag="O0286_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1410..2339
                     /locus_tag="O0286_002963"
     CDS             1410..2339
                     /locus_tag="O0286_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0171234.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2332..3069
                     /locus_tag="O0286_002964"
     CDS             2332..3069
                     /locus_tag="O0286_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0171235.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3083..3286
                     /locus_tag="O0286_002965"
     CDS             3083..3286
                     /locus_tag="O0286_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0171236.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1201 a          433 c          587 g         1078 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca gccatttaat atgactgtta
       61 caattactaa gattcaagca ctattacgcc gcacgtatga tttcaccact caagttaata
      121 atttaacagt agatgattgc gagttactac ttgatgcagc aaagcttaaa tttaatgaag
      181 aaaccataga cctgacacac actgaattgc aaatcatgca tagcttattc cagaacaagg
      241 gcaaattcgt cagtcgtaat gcgttaatcg aaaaatgctg ggaatcagaa cacttcattg
      301 atgacaatac gcttgctgtc aacattacac gtttacgcaa aaagctacaa aaactaggac
      361 ttacagattt tatcgaaacg aagaaaaatg tcggatatcg tgtaggtgac gtatgaattt
      421 cttgaaatca atccaacgtg aaatttatat tgtcatcctt acttgtgctt tattctactt
      481 tatattattc atgtataatg tgccgatgca agcatatatg attggtctag gtattgtatt
      541 gtttgtttca atgatttact ggggtatcca ttacatgaac tttaaaaaag aataaacact
      601 acaggaacag aacgaagatt taagaatcga aaaccataaa cttcgtaatg actttattaa
      661 ctaccaaagt gagttagagt catatttttt aatgtgggtg catcaaatca agacaccgat
      721 tacagcatca aagttactgc ttgaagatcc tgatgaacac gtgatttcta acgtgcgtaa
      781 tgaagtctta caaattgata attacacgaa tcttgcactg aactatttga aactcatgaa
      841 ccaaaagaca tacatggtct ttatggaagt gagtgtagat gaattaattc gaccactgat
      901 taaacgttat gcgatacagt ttattcatca tcaaacaaag ttgcattatg agaaaagtga
      961 agcggttata ttaacagatg ccaaatgggc atcaattatg attgagcaaa tattaaacaa
     1021 tgcattaaaa tatgcacgtg gcaaagatgt gtggattacg tttgacgaaa atacaaagaa
     1081 attatcaatc cgagataacg gtatcggcat taatgcgagt gatttaccga aaatctttga
     1141 taaaggtttc tcgggctata acggtagaat aagtgagaaa tcaagcggta ttggtttata
     1201 tattgtcagc acaatctcaa agcgcttagg ccataaagta agtgctacat ctacattaaa
     1261 cgatggttca atattcacca ttcaatttaa ataactccaa tctttcattt ttgtaagatt
     1321 agcggctgtt tttgtaagaa tagataaagg cagccttttc taatcgcgcg taagataaag
     1381 gtaacaacaa actaagaaag gtgccagagg tgaaaaattt gaataatata attgaaataa
     1441 ataacttaac aaagcaatat ggaagtcaga tagcagttaa taatttgagt ataactgttg
     1501 aaagtggaca aatttatggt tttttaggaa gaaatggtgc aggcaaaaca actactataa
     1561 gaatgctttt aggcttaatt aaacctacga aaggtaatat taaaatattt gaaaaagagt
     1621 tcgaaagtaa taaaactgaa attttaaaaa atattggtgc tatggttgaa acgccaggtt
     1681 tttatagtaa tttgacagct aaagagaact taactataaa tgctaaaata ttaggtatac
     1741 aaaaagaaaa tgcaattgaa gaagtgttag aaatagtagg actagcttct gaaaaagtaa
     1801 aattgtttaa aaattattct ttaggtatga agcaacgttt agggattgcg agagctttat
     1861 tgcacaatcc cgaattattg atacttgatg agccgacgaa tggattagat cctattggca
     1921 taaaagaaat tagaaagttg attaaggatc tggctcaatc tagaaatata accattttaa
     1981 tttcaagtca tatattgtca gaaatagagc agttagtaga caaggtgggt attattcata
     2041 acggagtttt gttagaagaa attatgtatg aagatttaaa aaaaaagaac agacaattta
     2101 taagtatatc agtatcagat gaagaaaaag ttgcatgttt gttggaaact aacttcggaa
     2161 tttatgacta taaaattaaa gaaaatggcg aatttaaaat ttattcacat attgatagac
     2221 aacaagaaat taatagatta ttagtattaa acaatattga tgttttccag atgagagtga
     2281 tagaagataa tctagaaact tatttcgaag acttagtagg aggtggcaca attggataat
     2341 ctactccgta cagaatttat taagttaaaa agatcatata tgtttctaat aagtatttta
     2401 ggtacatgcg tagcaccttt tatggtggtt gtagcttctt ttattagtat gcggacgaaa
     2461 gtaccgactc ctaatattgt atttaaacag ttatttgaag aagtcagttt atatcatatg
     2521 ctaattattg gacctcctct atatggggtt ataatagcct atttgtttgg aagagaatat
     2581 atagaagata ctttaaaaaa cttattaatt atacctatat ctagagaaag ttttttagta
     2641 agtaaaataa tattattatt cttgtggatt caaacactat ctgttattgc atggttgcta
     2701 actttaatgc taggtatgct attgcatttt caagggttaa gccagagttt aataattgat
     2761 tcattcttta catttgttat tggcggagtg ttattatgga tattatctcc accaataatt
     2821 attttgtcga tatattttaa aaattatgtt cccacaattg ttttaaccat aataattact
     2881 ttgataaatt taatgactgc taattctgaa catagaggat tatttccttg ggctgcagtt
     2941 ggagacatat ctagaggaac attacctaat acttatccat ttctattatc tttaattatc
     3001 ataattgtta catctatatt cagtgtaatt atattattct catactttaa aaaaactgat
     3061 ataagttaaa aggaggagaa aaatgggttt tatagagcag attaaagcat ttattttagg
     3121 tgtagttgaa gggttgacag aatttgctcc agtatcatca acaggtcata tgattattgt
     3181 tgatgatatg tggttaaaca ctgctgacga tttaggaagt gcgtctgcaa ctacttttaa
     3241 ggtggtaata cagttaggag gttctgttgc aaagttgaat ttataatata attttaaca
//
LOCUS       DAKTGS010000310         3226 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.195, whole genome shotgun sequence.
ACCESSION   DAKTGS010000310 DAKTGS010000000
VERSION     DAKTGS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.195"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            122..757
                     /locus_tag="O0286_002966"
                     /pseudo
     CDS             122..757
                     /locus_tag="O0286_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            754..1284
                     /gene="sat4"
                     /locus_tag="O0286_002967"
     CDS             754..1284
                     /gene="sat4"
                     /locus_tag="O0286_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0171237.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1377..2171
                     /locus_tag="O0286_002968"
                     /note="aph(3')-IIIa"
     CDS             1377..2171
                     /locus_tag="O0286_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0171238.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2447..3018
                     /locus_tag="O0286_002969"
                     /pseudo
     CDS             2447..3018
                     /locus_tag="O0286_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1078 a          508 c          736 g          904 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca tcatttttat taaaatactc
       61 ttttgtaaaa tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata
      121 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
      181 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
      241 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
      301 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
      361 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
      421 gatatcatgg aaggtcggca tcgaaacagg ctttaaatga agtgtaggca agaactataa
      481 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
      541 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
      601 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
      661 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
      721 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
      781 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
      841 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
      901 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
      961 actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg
     1021 cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg
     1081 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     1141 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     1201 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaaagct
     1261 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     1321 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     1381 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     1441 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     1501 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     1561 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     1621 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     1681 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     1741 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     1801 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     1861 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     1921 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     1981 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     2041 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     2101 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     2161 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     2221 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     2281 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     2341 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     2401 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     2461 cagagtaacc tctattctta ttcagctgca atcaaaaaaa taattcctgc caaagaaatt
     2521 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     2581 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     2641 cttctgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     2701 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     2761 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     2821 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     2881 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     2941 ccggaaagca gaatgataga gcctatatca ctggggtttt atgaacagaa ctggtactta
     3001 atcggttttg caggctaaga aatgaatata ggaattttcg tgtggatcgg attaaaagca
     3061 tttgcataga agaagagctc tgtcaaagcc atgatggctc tttggaacaa atattaaaac
     3121 aaatgctggt tctgttgcaa agtaaaaaaa tatagctaac cactaattta tcatgtcagt
     3181 gttcgcttaa cttgctagca tgatgctaat ttcgtggcat ggcgaa
//
LOCUS       DAKTGS010000311         2137 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.217, whole genome shotgun sequence.
ACCESSION   DAKTGS010000311 DAKTGS010000000
VERSION     DAKTGS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.217"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            61..768
                     /gene="deoD"
                     /locus_tag="O0286_002970"
     CDS             61..768
                     /gene="deoD"
                     /locus_tag="O0286_002970"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0171239.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            775..2127
                     /gene="tet(38)"
                     /locus_tag="O0286_002971"
     CDS             775..2127
                     /gene="tet(38)"
                     /locus_tag="O0286_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0171240.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT          665 a          323 c          393 g          756 t
ORIGIN      
        1 attatataca tggttagaca aatagacaaa tcactataca aatattggga ggaatatttt
       61 atgaaatcaa caccacacat taaaccaatg aatgacgtcg aaattgcaga aacggttcta
      121 ttgccaggag atccgttaag agctaagttc attgcagaaa cttatttgga tgatgtggaa
      181 cagttcaata cagtgcgaaa catgtttggt tttaccggaa catataaagg taaaaaagtt
      241 tctgtcatgg gttcaggtat gggtatgcca tctattggca tttactctta tgaattaatt
      301 catacatttg gttgtaaaaa attaattcgc gttggctctt gtggcgcgat gcaagaaaac
      361 attgatttat atgatgtgat tattgcacaa ggtgcctcta ctgattcaaa ttacgttcaa
      421 caatatcaat taccaggtca ttttgcgcca attgcttctt atcaattatt agaaaaagca
      481 gttgaaacag cacgtgacaa aggtgtacgt catcatgtag gtaatgtgtt atcaagtgat
      541 attttctata acgcggatac aacagcgagt gaacgttgga tgcgtatggg tattttaggt
      601 gtagaaatgg aatcagctgc attatacatg aatgcaattt acgctggtgt cgaagcatta
      661 ggtgtgttca cagtgagcga tcatttaatt catgaaacgt caacaacacc tgaggaaagg
      721 gaacgtgcat ttacagatat gattgaaatt gcactgtcat tggtgtagat gattatgaat
      781 gttgaatatt ctaaaataaa gaaagcagta cctattttat tattcttatt tgtattcagt
      841 ttggttatag acaactcatt taaattgatt tctgtagcca ttgctgatga cttaaacata
      901 tctgtaacga cagtaagttg gcaagcgaca ttagccggtt tagtaattgg tattggcgct
      961 gtagtatacg cttcattatc tgatgccatt agtatacgca cactatttat ttatggcgtg
     1021 atattaatca ttatcggatc aattattggt tacattttcc aacatcaatt cccattactt
     1081 ttagttggac gtattattca aactgccggt ttagctgctg cagagacatt atatgtgata
     1141 tatgttgcaa agtatctttc taaagaggac cagaagactt accttggctt aagtacgagc
     1201 agttattcct tgtcattagt tatcggtaca ttatcaggtg gatttatttc tacgtattta
     1261 cactggacaa atatgttttt aattgcatta atcgtagtat ttacgttgcc attcctattt
     1321 aaattattac caaaagaaaa taatacgaat aaagctcatt tagattttgt tggcttaatt
     1381 ctagtggcaa ctattgctac aacagtcatg ctgtttatta cgaactttaa ttggttatat
     1441 atgattggtg ccttaattgc gattatcgtt tttgcgctat atattaaaaa tgcgcaacgt
     1501 ccattagtaa ataaatcatt tttccaaaat aaacgttatg cttcattttt atttatagta
     1561 tttgtaatgt atgctatcca attgggttat atttttacgt tcccattcat aatggagcaa
     1621 atttatcatc tgcaactaga cacaacatca ctgttattag taccgggtta tatagtagca
     1681 gtcattgttg gtgcactaag tggtaaaatc ggcgaatatc tgaattcaaa acaagcgatt
     1741 atcacagcaa ttattttaat agcactgagc ttgattttac ctgcatttgc agtaggtaat
     1801 cacatttcaa tcttcgtcat ttctatgata ttctttgcag gtagctttgc tttaatgtat
     1861 gcacctttac ttaacgaagc cattaaaaca atagatctta atatgacagg tgtggctatt
     1921 ggtttttata atttaattat taatgtggcg gtatctgtag gtattgcgat tgctgcggct
     1981 ctaatcgatt ttaaagcatt aaatttccca ggcaatgatg cattaagttc acatttcggt
     2041 attattttaa ttattttagg tttaatgagt attgtcggat tagttttatt cgtcagctta
     2101 aatcgttgga cacaatctga aaaataaata gatataa
//
LOCUS       DAKTGS010000312         1473 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 1132
            SAMN04868170-rid16188653.guided.246, whole genome shotgun sequence.
ACCESSION   DAKTGS010000312 DAKTGS010000000
VERSION     DAKTGS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868170
            Sequence Read Archive: SRR5415297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516869.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516869.1
            Genome Coverage       :: 97x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,909
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 34 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 13 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868170-rid16188653.guided.246"
                     /isolate="1132"
                     /isolation_source="Perineum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: CA"
                     /collection_date="2003"
                     /collected_by="RGoering"
     gene            <1..855
                     /locus_tag="O0286_002972"
     CDS             <1..855
                     /locus_tag="O0286_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0171241.1"
                     /translation="AIVKYNSFTKAAQFLHISQPSLTATIKKMEADLGYDLFTRSTKD
                     IKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTNQWIANLIRKH
                     HSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPLYEESYILLAP
                     KETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETDRFESAVGFVH
                     LGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQVHTFVQQCQDY
                     LYGLLEAL"
     gene            977..1396
                     /locus_tag="O0286_002973"
     CDS             977..1396
                     /locus_tag="O0286_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0171242.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT          573 a          231 c          216 g          453 t
ORIGIN      
        1 gctatcgtga aatataatag ttttactaaa gctgcacaat ttttacatat tagccagcca
       61 tctttaactg ctacgattaa aaaaatggaa gcagatttag gttatgactt atttacacgt
      121 tcaacaaaag acatcaagat taccgaaaaa ggaatacagt tttatcgtta tgcgagcgaa
      181 ttagttcaac aatatcgatc cacgatggaa aaaatgtatg atttaagcgt tacatcagaa
      241 ccaaggataa aaattgggac tcttgaatct acgaatcaat ggattgcgaa tttaattcga
      301 aagcaccatt ccgactaccc tgaacagcaa tatcgtttat atgaaataca tgataaacat
      361 caatctatag agcaattact gaattttaat attcatttag ctataacaaa tgaaaaaata
      421 acccacgaag atataagatc cattccttta tatgaggaat cttacatttt attagcaccc
      481 aaggaaacat ttaaaaatca aaattgggta gatgttgaaa atttgccact catattacca
      541 aacaaaaatt ctcaagtgcg caaacactta gatgactatt ttaatagaag aaatattcgt
      601 ccaaatgtcg ttgtagaaac agatcgattc gaatcagcag ttggatttgt tcatctcggc
      661 ttaggttacg ctatcattcc gagattttat taccaatcat ttcacacgtc taatttagaa
      721 tataaaaaaa ttcgtccaaa cttaggccga aaaatttata tcaattacca taaaaaacgc
      781 aaacactccg aacaagtaca tacattcgta caacaatgcc aagattattt atatggactt
      841 ttagaggctc tttaacttaa gttattagag cctcttatgc agttgcgcag atcatcgtat
      901 aaaaattaat gacgtcattt caaaaatcga tacaaaaata atttattata aaaattctaa
      961 gaaagaagtg aagcagatgt taaaatctat taatcatata tgcttttcag tcagaaattt
     1021 aaacgattca atacattttt atagagatat tttacttggg aaattgctat tgactggtaa
     1081 aaaaactgct tattttgagc ttgcaggcct atggattgct ttaaatgaag aaaaagatat
     1141 accacgtaat gaaattcact tttcatatac acatatagct ttcactatag atgacagcga
     1201 atttaaatat tggcatcaga ggttaaaaga taataacgtg aatattttag aaggaagagt
     1261 tagagatatt agagatagac aatcaattta ctttaccgac cctgatggtc ataagctaga
     1321 attacatact ggcacacttg agaacagatt aaattattat aaagaggcta aaccacatat
     1381 gacattttac aaataaggtg tcattataaa aaggcctctt gaactccgtt aaaattttaa
     1441 ttaattatta tataataaga gaacttttca aac
//
